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<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P0T5</accession>
    <accession>A0A6I8W2B0</accession>
    <name>A0A0R3P0T5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Innexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">shakB</name>
        <name evidence="12" type="synonym">inx</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Structural component of the gap junctions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the pannexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033237491.1">
        <property type="nucleotide sequence ID" value="XM_033381600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P0T5"/>
    <dbReference type="FunCoup" id="A0A0R3P0T5">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P0T5"/>
    <dbReference type="InParanoid" id="A0A0R3P0T5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247033">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005921">
        <property type="term" value="C:gap junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000990">
        <property type="entry name" value="Innexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11893">
        <property type="entry name" value="INNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11893:SF40">
        <property type="entry name" value="INNEXIN SHAKING-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00876">
        <property type="entry name" value="Innexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01262">
        <property type="entry name" value="INNEXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51013">
        <property type="entry name" value="PANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0303">Gap junction</keyword>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU010713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033237491.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="61100" checksum="94540D5556ED2A24" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAU2</accession>
    <name>A0A6I8UAU2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LON peptidase N-terminal domain and RING finger protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4811920</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352523.4">
        <property type="nucleotide sequence ID" value="XM_001352487.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAU2"/>
    <dbReference type="FunCoup" id="A0A6I8UAU2">
        <property type="interactions" value="476"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811920"/>
    <dbReference type="InParanoid" id="A0A6I8UAU2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAU2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16513">
        <property type="entry name" value="RING-HC_LONFs_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16514">
        <property type="entry name" value="RING-HC_LONFs_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF42">
        <property type="entry name" value="LON PEPTIDASE N-TERMINAL DOMAIN AND RING FINGER PROTEIN C14F5.10C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="248"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="769"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="Lon N-terminal" evidence="7">
        <location>
            <begin position="896"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="826"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1103"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="826"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="841"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1109"/>
            <end position="1118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001352523.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1170" mass="128173" checksum="8F7AF40E1FEF72ED" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAV9</accession>
    <name>A0A6I8UAV9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing G-protein coupled receptor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4812038</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352546.3">
        <property type="nucleotide sequence ID" value="XM_001352510.4"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A6I8UAV9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="529"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001352546.3"/>
        </source>
    </evidence>
    <sequence length="578" mass="65178" checksum="C53790A7B7863495" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UC91</accession>
    <name>A0A6I8UC91_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Reversion-inducing cysteine-rich protein with Kazal motifs</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Reck</name>
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    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_001353149.4"/>
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        <property type="interactions" value="501"/>
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    <dbReference type="InParanoid" id="A0A6I8UC91"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:extracellular matrix organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CC4_RECK"/>
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        <property type="entry name" value="EGF1_RECK"/>
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        <property type="entry name" value="FnI_RECK"/>
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        <property type="entry name" value="FZ_RECK"/>
    </dbReference>
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        <property type="entry name" value="Kazal_dom"/>
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        <property type="entry name" value="Kazal_dom_sf"/>
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        <property type="entry name" value="RECK-like_N"/>
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        <property type="entry name" value="REVERSION-INDUCING CYSTEINE-RICH PROTEIN WITH KAZAL MOTIFS"/>
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    <dbReference type="PANTHER" id="PTHR13487">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR"/>
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    </dbReference>
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        <property type="entry name" value="CC4_RECK"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF25027">
        <property type="entry name" value="EGF1_RECK"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="KAZAL_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026199879" evidence="2">
        <location>
            <begin position="24"/>
            <end position="1080"/>
        </location>
    </feature>
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        <location>
            <begin position="729"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353185.3"/>
        </source>
    </evidence>
    <sequence length="1080" mass="118497" checksum="3E4D4309D48104FF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCK4</accession>
    <name>A0A6I8UCK4_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Cuticle protein LPCP-23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4813315</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353247.3">
        <property type="nucleotide sequence ID" value="XM_001353211.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCK4"/>
    <dbReference type="KEGG" id="dpo:4813315"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="F:structural constituent of cuticle"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="LARVAL/PUPAL CUTICLE PROTEIN H1C-LIKE PROTEIN-RELATED"/>
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        <property type="entry name" value="MIP24391P"/>
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    <dbReference type="Pfam" id="PF11018">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="202"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353247.3"/>
        </source>
    </evidence>
    <sequence length="202" mass="21784" checksum="0529061A13347773" modified="2020-08-12" version="1">MAHQFLSFAASLLLILAPAWAGIIAQPTIGYYGDEHAVAHTQQNVVRSFDGTVSHYAKSVATPYSQVHKQDTRISNNVYQPAIAKTVTYAAPAPAAVYNHHAHPEPQLFTQAAPQAAVYHQAVQAAPVANVYHQEAPQASVYHQAAPQAAVYHQAAPQASVYHQAAPQASVYHQAQPAVIHYSPAESVSHMSFDGFGTHWGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFG0</accession>
    <name>A0A6I8UFG0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein cramped</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">crm</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
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        </source>
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        <property type="expression patterns" value="baseline"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cramped-like"/>
    </dbReference>
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        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21677">
        <property type="entry name" value="CRAMPED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21677:SF1">
        <property type="entry name" value="PROTEIN CRAMPED-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="129"/>
            <end position="190"/>
        </location>
    </feature>
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        <location>
            <begin position="90"/>
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        </location>
    </feature>
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        <location>
            <begin position="345"/>
            <end position="382"/>
        </location>
    </feature>
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        <location>
            <begin position="432"/>
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        </location>
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        <location>
            <begin position="805"/>
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    </feature>
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        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
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        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
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        <location>
            <begin position="438"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="814"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001354647.4"/>
        </source>
    </evidence>
    <sequence length="951" mass="104454" checksum="D4D4A6072A6D54D0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH59</accession>
    <name>A0A6I8UH59_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Gastrula zinc finger protein XlCGF8.2DB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4815867</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355460.2">
        <property type="nucleotide sequence ID" value="XM_001355424.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UH59"/>
    <dbReference type="FunCoup" id="A0A6I8UH59">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815867"/>
    <dbReference type="InParanoid" id="A0A6I8UH59"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001049">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001442">
        <property type="entry name" value="zinc finger protein 696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF111">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="269"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355460.2"/>
        </source>
    </evidence>
    <sequence length="331" mass="38970" checksum="89FF66CA9D40F1FD" modified="2020-08-12" version="1">MAMETLEDVSFDWDSVIEECEESKDSEQEWVNSIIIEEPEEELADMTVHQDEAEQAVDDPEDWMIEQEVEELEPGECRPEPSVTPCHLPWVCTRCPLRFTSHEKLIAHRRIHQRTQRDIRPPQSIFPCEECGKEFKRRRWLDDHLHTHTGDRPYQCSDCGARFAQNSNLSAHIRTKHLKEALHQCPNCPDRRFTRRRLLQQHLKTVHQKLRDLVCEQCGASFSHPTYLKKHMLDHSSIKAYCCHICGKQFGRLENRNSHLYVHSTRKPYICSVCGIGFSRKSHLVQHIEFKNHPNEVIQRQKPHFSDLLSQFTRKKDTEAKNGLEFQLNCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHJ3</accession>
    <name>A0A6I8UHJ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RCR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">hiw</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine.</text>
            <dbReference type="EC" id="2.3.2.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-Cys relay (RCR) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.33" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001355609.4">
        <property type="nucleotide sequence ID" value="XM_001355573.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UHJ3">
        <property type="interactions" value="2064"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816080"/>
    <dbReference type="InParanoid" id="A0A6I8UHJ3"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHJ3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099174">
        <property type="term" value="P:regulation of presynapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19799">
        <property type="entry name" value="Bbox2_MYCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16463">
        <property type="entry name" value="RING-H2_PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000078">
        <property type="entry name" value="E3 ubiquitin-protein ligase MYCBP2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000051">
        <property type="entry name" value="Highwire, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.820">
        <property type="entry name" value="PHR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012983">
        <property type="entry name" value="PHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038648">
        <property type="entry name" value="PHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45943">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MYCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45943:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MYCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08005">
        <property type="entry name" value="PHR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="RCC1" evidence="14">
        <location>
            <begin position="985"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="DOC" evidence="17">
        <location>
            <begin position="4478"/>
            <end position="4657"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="5321"/>
            <end position="5372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="924"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1049"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1231"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1695"/>
            <end position="1794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2299"/>
            <end position="2332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2570"/>
            <end position="2622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="3223"/>
            <end position="3339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="3362"/>
            <end position="3387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="3428"/>
            <end position="3516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="3614"/>
            <end position="3638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="3730"/>
            <end position="3769"/>
        </location>
    </feature>
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    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF56">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="135"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355865.2"/>
        </source>
    </evidence>
    <sequence length="418" mass="48356" checksum="B29C86B2061DFD5C" modified="2020-08-12" version="1">MCSIEYDAEVAAPPKHLDVAFFEEVLETALRTARGVQLLSIHIRMGSSTGENYCSQIYRTKVVYRLPGQADQHMMFIVKSIPRVESVEFIEDLQVYLKEKVTYHDVLPRLELLMKCKRRFGPKLFQCLKQPENTLVFEDLGHSGFVMASRESGLNEEHCRLVMERLAEFHATSMALAVLDPHIFEAYSDGMLSPNGLAKDDGMLMRFFADNGEELHTVVSSWPGYERIAEKIGKYMKNQRSNLVRAQALQAKEIRVLNHGDLWVNNLLFKYDNAKRPQDLIFIDFQLSVWGSPGIDLNYFFYTSLSLEVLRHNRPMLLRTYHTRLSETLLNLDLGIPVPSYEQILEEVHRRECYGFFASYGIFPTVSQDKSQTADNNLENFTDANFAKQKVRQMFESKRLRETLRYTLPHFERAGVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIM5</accession>
    <name>A0A6I8UIM5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-alpha-acetyltransferase 20</fullName>
            <ecNumber evidence="5">2.3.1.254</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Methionine N-acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">N-acetyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">N-terminal acetyltransferase B complex catalytic subunit NAA20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">N-terminal acetyltransferase B complex catalytic subunit NAT5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">NatB catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Naa20B</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N-terminal L-methionyl-L-asparaginyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-asparaginyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50484"/>
            <dbReference type="Rhea" id="RHEA-COMP:12694"/>
            <dbReference type="Rhea" id="RHEA-COMP:12695"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133356"/>
            <dbReference type="ChEBI" id="CHEBI:133358"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-terminal L-methionyl-L-aspartyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-aspartyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50480"/>
            <dbReference type="Rhea" id="RHEA-COMP:12692"/>
            <dbReference type="Rhea" id="RHEA-COMP:12693"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133045"/>
            <dbReference type="ChEBI" id="CHEBI:133063"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N-terminal L-methionyl-L-glutaminyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50492"/>
            <dbReference type="Rhea" id="RHEA-COMP:12698"/>
            <dbReference type="Rhea" id="RHEA-COMP:12699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133361"/>
            <dbReference type="ChEBI" id="CHEBI:133362"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-terminal L-methionyl-L-glutamyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutamyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50488"/>
            <dbReference type="Rhea" id="RHEA-COMP:12696"/>
            <dbReference type="Rhea" id="RHEA-COMP:12697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133359"/>
            <dbReference type="ChEBI" id="CHEBI:133360"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the N-terminal acetyltransferase B (NatB) complex which is composed of NAA20 and NAA25.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.254" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001356127.2">
        <property type="nucleotide sequence ID" value="XM_001356091.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIM5"/>
    <dbReference type="FunCoup" id="A0A6I8UIM5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816445"/>
    <dbReference type="InParanoid" id="A0A6I8UIM5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001356127.2"/>
        </source>
    </evidence>
    <sequence length="203" mass="23380" checksum="015241DD4315E15D" modified="2020-08-12" version="1">MTTLRGLVADDLFKFNSIVLEEFVEGYGILFYLSKMTENPALCQAAVAPDGRLIGVLVGTHAVNKNEKIQSENGPDLHPTCGHISMLAVASDYRRLGLGTCLMGHFMEIVERYSDWYVDLFVRQSNVSAIQLYKSLGFVEYRFLPMYYIDENGFELRMPLSRDVERMSLKGFSTNVYYFAYQVILNLIKQCLEYLKDRLSWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK43</accession>
    <name>A0A6I8UK43_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homogentisate 1,2-dioxygenase</fullName>
            <ecNumber evidence="5">1.13.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Homogentisate oxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Homogentisic acid oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Homogentisicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">hgo</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>homogentisate + O2 = 4-maleylacetoacetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15449"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
            <dbReference type="ChEBI" id="CHEBI:17105"/>
            <dbReference type="EC" id="1.13.11.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the homogentisate dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.5" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001356795.3">
        <property type="nucleotide sequence ID" value="XM_001356759.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK43"/>
    <dbReference type="FunCoup" id="A0A6I8UK43">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816909"/>
    <dbReference type="InParanoid" id="A0A6I8UK43"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00339"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK43">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004411">
        <property type="term" value="F:homogentisate 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07000">
        <property type="entry name" value="cupin_HGO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000026">
        <property type="entry name" value="Homogentisate 1,2-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046451">
        <property type="entry name" value="HgmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046452">
        <property type="entry name" value="HgmA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005708">
        <property type="entry name" value="Homogentis_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01015">
        <property type="entry name" value="hmgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056:SF0">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04209">
        <property type="entry name" value="HgmA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20510">
        <property type="entry name" value="HgmA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11 17" id="KW-0408">Iron</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase N-terminal" evidence="19">
        <location>
            <begin position="5"/>
            <end position="279"/>
        </location>
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        <location>
            <begin position="280"/>
            <end position="434"/>
        </location>
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        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="335"/>
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        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="341"/>
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        <ligand>
            <name>Fe cation</name>
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        <location>
            <position position="350"/>
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        <ligand>
            <name>homogentisate</name>
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        <location>
            <position position="371"/>
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        <ligand>
            <name>Fe cation</name>
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        <location>
            <position position="371"/>
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        <ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00000076"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004704"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007757"/>
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            <dbReference type="Pfam" id="PF20510"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001356795.3"/>
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    <sequence length="441" mass="49482" checksum="7B4F064CBA7005BD" modified="2020-08-12" version="1">MSQYKYLSGFGSHFSSEDERYPNSLPLGQNSPQVCPYKLYAEQLSGSAFTAPRTENVRTWLYRKLPSAVHQPFQPFKGATYFKQNWDEQPPNPNQLRWKPFDLPPQQGRKITFVEGLHTVCGAGDPRARHGLAIHIYSCNESMDNSAFYNSDGDFLIVPQQGILDITTEMGRMKVAPNEICVIPQGIRFAVSVNGPSRGYILEVYDGHFVLPDLGPIGANGLANPRDFETPVAWFNDQPIEDFQVISKFQGSLFVAQQNHSVFDVVAWHGNYVPYKYDLSKFMVINSVSFDHCDPSIFTVLTCPSLRAGTAIADFVIFPPRWSVQEHTFRPPYYHRNCMSEFMGLILGKYEAKEDGFAAGGATLHSIMTPHGPDVKCFDGASNAKLVPERIAEGTQAFMFESSLSLAVTKWGEDTCQKLDAAYYECWQALKDNFTLGKAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKL4</accession>
    <name>A0A6I8UKL4_DROPS</name>
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            <fullName evidence="4">Uncharacterized protein</fullName>
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        <name evidence="4" type="primary">LOC4817601</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Endopterygota</taxon>
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            <taxon>Sophophora</taxon>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Mediator_comp_sub12"/>
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        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026212899" evidence="2">
        <location>
            <begin position="35"/>
            <end position="454"/>
        </location>
    </feature>
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        <location>
            <begin position="58"/>
            <end position="133"/>
        </location>
    </feature>
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            <begin position="410"/>
            <end position="454"/>
        </location>
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            <begin position="58"/>
            <end position="70"/>
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            <begin position="410"/>
            <end position="420"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357003.3"/>
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    <sequence length="454" mass="50255" checksum="B54FA3C7FFE904C9" modified="2020-08-12" version="1">MSLSISLRKLEGNSQMLMMLLLVLSPLILGQVMAVPASVLAQNTELSTNANNKLQLQQLEQQQQQQEQQQPAAAKRSEDTSAKAMVPTANKKSSPEIVPSSFSNSPPARSSNKILSNQQQQQQQLEKGPSGIYALPSNDEILAAVAAAAQNSQQQVQEEPTALEEAVASSTMRKRGINYDYNPYMAPNDYGSDVPNGVWADEYDTAVPVSYGERDLQDLDDYVPDRRVGGSIGRNKAYDNLQNLLNAEAYLESIPLSVPLTYANRNYNLDDRNKRGGLFYNMGSTGGSMQGSTGTGSGLSNDNINLSKYRRFNDMRLKRDTTKLNPADMLALVALVEAGERARKESDVESAGPVPMIASDDMDYVPAGTWLDAPVQQQQPGMMDYYMPLENQNQAMPQYEYVPRQHKYSGVNSRFGSSKQRYMVSKKKRSISQSQFMNEPVAERGSSYNGEKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULM8</accession>
    <name>A0A6I8ULM8_DROPS</name>
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        <submittedName>
            <fullName evidence="10">T-box protein H15</fullName>
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        <name evidence="10" type="primary">mid</name>
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    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="10">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357470.3">
        <property type="nucleotide sequence ID" value="XM_001357434.4"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="term" value="P:cell fate specification"/>
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        <property type="term" value="P:heart development"/>
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        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="209"/>
            <end position="400"/>
        </location>
    </feature>
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    </feature>
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            <begin position="531"/>
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        </location>
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        </location>
    </feature>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357470.3"/>
        </source>
    </evidence>
    <sequence length="600" mass="65162" checksum="CF3B8E0B5978E26E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULU1</accession>
    <name>A0A6I8ULU1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MD-2-related lipid-recognition protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4800310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357597.2">
        <property type="nucleotide sequence ID" value="XM_001357560.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULU1"/>
    <dbReference type="SMR" id="A0A6I8ULU1"/>
    <dbReference type="FunCoup" id="A0A6I8ULU1">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800310"/>
    <dbReference type="InParanoid" id="A0A6I8ULU1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070966">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00916">
        <property type="entry name" value="Npc2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.770:FF:000001">
        <property type="entry name" value="NPC intracellular cholesterol transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033916">
        <property type="entry name" value="ML_Npc2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF55">
        <property type="entry name" value="GEO08227P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026092745" evidence="6">
        <location>
            <begin position="25"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="7">
        <location>
            <begin position="29"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001357597.2"/>
        </source>
    </evidence>
    <sequence length="160" mass="17412" checksum="7B2D9823B7F3BF57" modified="2020-08-12" version="1">MLRLGSLLLLLALAIVSVSVSVHGESINFRNCADSVDVCTVDQVRVSPCPQAASNAACHIRRRRPAVMSFDFTPQFDADSLGATLGWVKDENTELPLLSLERDACKATGCPVRSGVQATYTTEVPIEAKFPLSAYTIRWALKDPASGKRCCFLIDIKVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULX8</accession>
    <name>A0A6I8ULX8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4800309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357598.2">
        <property type="nucleotide sequence ID" value="XM_001357561.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULX8"/>
    <dbReference type="SMR" id="A0A6I8ULX8"/>
    <dbReference type="FunCoup" id="A0A6I8ULX8">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800309"/>
    <dbReference type="InParanoid" id="A0A6I8ULX8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001357598.2"/>
        </source>
    </evidence>
    <sequence length="289" mass="33461" checksum="AD47BCCFC6AFA680" modified="2020-08-12" version="1">MSFPKTFDKLLLVEYGAAFMYYMEKHKLLEVISRLLAEVSEQPVEDLRHWMGANVRRIGIDIYTKHLDAVHRGVPGDFYQLPKTCLHRLVLHGRPGSGRRSLAHVLAKRWDLLIIDADVLAYHSINGHRQDENTGQLQDAIEEDCVFGRSEAIGRIVQERLLKEDALHRGWILINYPNNRCEARELFECATVPPNRLIFLQINERMARMRILTKSYAAGPQANYTYLDRQMQQFRKSEPLLNAYLSQRREVLYVDATPCFEEVKCHIISQLTKTPYVLGFKYGEANATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMC1</accession>
    <name>A0A6I8UMC1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4800578</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357829.4">
        <property type="nucleotide sequence ID" value="XM_001357792.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMC1"/>
    <dbReference type="FunCoup" id="A0A6I8UMC1">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800578"/>
    <dbReference type="InParanoid" id="A0A6I8UMC1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01099">
        <property type="entry name" value="PAN_AP_HGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052774">
        <property type="entry name" value="Celegans_DevNeuronal_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056953">
        <property type="entry name" value="CUT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057371">
        <property type="entry name" value="VERL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327:SF8">
        <property type="entry name" value="FI17836P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327">
        <property type="entry name" value="FI18240P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25057">
        <property type="entry name" value="CUT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25272">
        <property type="entry name" value="VERL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="799"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="203"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="316"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="5">
        <location>
            <begin position="404"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="821"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001357829.4"/>
        </source>
    </evidence>
    <sequence length="858" mass="97730" checksum="D87754049D94588D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMW1</accession>
    <name>A0A6I8UMW1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL32m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L32, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mRpL32</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the mitochondrial large ribosomal subunit (mt-LSU). The mitochondrial ribosome (mitoribosome) is a large ribonucleoprotein complex responsible for the synthesis of proteins inside mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357937.2">
        <property type="nucleotide sequence ID" value="XM_001357900.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMW1"/>
    <dbReference type="FunCoup" id="A0A6I8UMW1">
        <property type="interactions" value="437"/>
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    <dbReference type="KEGG" id="dpo:4800712"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051991">
        <property type="entry name" value="Mitoribosomal_protein_bL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21026:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL32M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001357937.2"/>
        </source>
    </evidence>
    <sequence length="198" mass="22847" checksum="3761C6A12603F8FA" modified="2020-08-12" version="1">MSKNLILSLTNFVRSLERLFLPYGGHPPALALAGFQHEHSTSSNKRAQEFSLKELIGDGMLWAVPKHRRSVEKRLNRKFGYPEYVWKPLKVKRNIRSCQTCGHDHELGVLCPFCYNKVLKETEQMQAKIQEKLGLDPVDKEVIVLYEGEKAEQTAEQLQGKRIVEMKKPRPMWFTKNLLQKSTQQQSEAKEVKPSDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNA1</accession>
    <name>A0A6I8UNA1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein CG4951 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4801102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358251.4">
        <property type="nucleotide sequence ID" value="XM_001358214.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNA1"/>
    <dbReference type="FunCoup" id="A0A6I8UNA1">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801102"/>
    <dbReference type="InParanoid" id="A0A6I8UNA1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UNA1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358251.4"/>
        </source>
    </evidence>
    <sequence length="538" mass="61113" checksum="00951EDE3793A96A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UND6</accession>
    <name>A0A6I8UND6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Tissue inhibitor of metalloproteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Timp</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358207.3">
        <property type="nucleotide sequence ID" value="XM_001358170.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UND6"/>
    <dbReference type="FunCoup" id="A0A6I8UND6">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801043"/>
    <dbReference type="InParanoid" id="A0A6I8UND6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03577">
        <property type="entry name" value="NTR_TIMP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030490">
        <property type="entry name" value="TIMP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF33">
        <property type="entry name" value="TISSUE INHIBITOR OF METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00288">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="4" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026202316" evidence="12">
        <location>
            <begin position="28"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="13">
        <location>
            <begin position="28"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="30"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="40"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="145"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="150"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358207.3"/>
        </source>
    </evidence>
    <sequence length="210" mass="23953" checksum="B8B0C111291F951C" modified="2020-08-12" version="1">MDFRKYLGLLALVLLAVLTLNGRPVDACSCMPSHPQTHFCKADYVVQLRVLRKSDTIDPGKTTYKVHIKRTYKATPEARRMLRDGRLATPNSDSMCGIRLDLGKVYIIAGRMPTLNVCSYYKEYTKMTITERHGFSGGYGKSCNCTVSPCFGQQCLRHREYSDGCKWSPFGKCERDYSACMPHVTHTVNGSISRCRWRRTQLYRKCLSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPD6</accession>
    <name>A0A6I8UPD6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="11">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">wat</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001358673.2">
        <property type="nucleotide sequence ID" value="XM_001358636.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPD6"/>
    <dbReference type="FunCoup" id="A0A6I8UPD6">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801606"/>
    <dbReference type="InParanoid" id="A0A6I8UPD6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000143">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF60">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="13">
        <location>
            <begin position="43"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="12">
        <location>
            <begin position="383"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358673.2"/>
        </source>
    </evidence>
    <sequence length="518" mass="59791" checksum="233CBEAB5AC07D58" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPJ0</accession>
    <name>A0A6I8UPJ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SMC1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358804.2">
        <property type="nucleotide sequence ID" value="XM_001358767.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPJ0"/>
    <dbReference type="FunCoup" id="A0A6I8UPJ0">
        <property type="interactions" value="1650"/>
    </dbReference>
    <dbReference type="GeneID" id="4801763"/>
    <dbReference type="KEGG" id="dpo:4801763"/>
    <dbReference type="InParanoid" id="A0A6I8UPJ0"/>
    <dbReference type="OMA" id="KHMDFQR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079325">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03275">
        <property type="entry name" value="ABC_SMC1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1060.20:FF:000001">
        <property type="entry name" value="Structural maintenance of chromosomes 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000564">
        <property type="entry name" value="Structural maintenance of chromosomes 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028468">
        <property type="entry name" value="Smc1_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF12">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="13">
        <location>
            <begin position="535"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="406"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="893"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="465"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="697"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1037"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358804.2"/>
        </source>
    </evidence>
    <sequence length="1238" mass="142855" checksum="41969B210A33DE36" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ33</accession>
    <name>A0A6I8UQ33_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein light chain roadblock</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359067.1">
        <property type="nucleotide sequence ID" value="XM_001359030.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ33"/>
    <dbReference type="FunCoup" id="A0A6I8UQ33">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="4802075"/>
    <dbReference type="KEGG" id="dpo:4802075"/>
    <dbReference type="InParanoid" id="A0A6I8UQ33"/>
    <dbReference type="OMA" id="PTNKLCY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076159">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016561">
        <property type="entry name" value="DYNLRB1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009998">
        <property type="entry name" value="DLC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR009998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359067.1"/>
        </source>
    </evidence>
    <sequence length="101" mass="11169" checksum="EBDF818E1B0BA23E" modified="2020-08-12" version="1">MNRILEDVIHKNAATVERITSENTAGYVVADNTANAVAETSFDNTGAQAIVKHLHGVLVTSCQSVIRDLDPTNKLCFLRVATRKFEYLVAPEEYFTITVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ73</accession>
    <name>A0A6I8UQ73_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Enhancer of split m3 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">E(spl)m3-HLH</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359175.2">
        <property type="nucleotide sequence ID" value="XM_001359138.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ73"/>
    <dbReference type="FunCoup" id="A0A6I8UQ73">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:5384074"/>
    <dbReference type="InParanoid" id="A0A6I8UQ73"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19741">
        <property type="entry name" value="bHLH-O_ESMB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000009">
        <property type="entry name" value="Transcription factor HES-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="11"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="8">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359175.2"/>
        </source>
    </evidence>
    <sequence length="223" mass="24504" checksum="A5AF98AF1B2A4BEE" modified="2020-08-12" version="1">MVMEMSKTYQYRKVMKPLLERKRRARINKCLDDLKDLMVECLQQEGEHVTRLEKADILELTVDHMRKLKQRGGLSLQGPVAGGSPPPATSTAHVESFRSGYVHAADQITQVLLQTQQTDEIGRKIMKFLSTRLIELQTQLLTQQQQQQQQSGPAVGFASGRLAFPLLGGYGPAAAAAAVSYSAYLSGKDELIDVTSVDGTAISETASVSSQESGASEPVWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQH6</accession>
    <name>A0A6I8UQH6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase 16 kDa proteolipid subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">V-type proton ATPase 21 kDa proteolipid subunit c''</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Vacuolar proton pump 16 kDa proteolipid subunit c</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">LOC4802391</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15 16">MV-25-SWS-2005</strain>
            <strain evidence="14 15">MV2-25</strain>
            <tissue evidence="15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359317.1">
        <property type="nucleotide sequence ID" value="XM_001359280.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037770.1">
        <property type="nucleotide sequence ID" value="XM_015182284.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQH6"/>
    <dbReference type="GeneID" id="4802391"/>
    <dbReference type="KEGG" id="dpo:4802391"/>
    <dbReference type="OMA" id="TSPYMWG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080021">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18177">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18178">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000002">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="54"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="143"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001359317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015037770.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="21969" checksum="C923FA9799DE137C" modified="2020-08-12" version="1">MAAQIRTVVTSTFLGLFTAAATILILYFVMTGKGERVSVGWFLTSSNPYMWACLGIGLAVSLSVVGAALGIHTTGTSIVGGGVKAPRIKTKNLISVIFCEAVAIYGLITAIVLSGQLEQFQMETALNNVAIMNNNWFSGYLIFGAGLAVGLVNLFCGIAVGIVGSGAALSDAANAALFVKILIVEIFGSAIGLFGLIVGIYMTSKSKMGDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQV2</accession>
    <name>A0A6I8UQV2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359367.2">
        <property type="nucleotide sequence ID" value="XM_001359330.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQV2"/>
    <dbReference type="SMR" id="A0A6I8UQV2"/>
    <dbReference type="FunCoup" id="A0A6I8UQV2">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="GeneID" id="4802449"/>
    <dbReference type="KEGG" id="dpo:4802449"/>
    <dbReference type="InParanoid" id="A0A6I8UQV2"/>
    <dbReference type="OMA" id="GDKMHIE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071214">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd01794">
        <property type="entry name" value="Ubl_UBTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.225.20">
        <property type="entry name" value="Ub domain-containing protein, DC-UbP/UBTD2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032752">
        <property type="entry name" value="DC-UbP/UBTD2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038169">
        <property type="entry name" value="DC-UbP/UBTD2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039869">
        <property type="entry name" value="UBTD1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13609">
        <property type="entry name" value="UBIQUITIN DOMAIN CONTAINING 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16455">
        <property type="entry name" value="UBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="161"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359367.2"/>
        </source>
    </evidence>
    <sequence length="252" mass="27853" checksum="F282B161125EDDB6" modified="2020-08-12" version="1">MGACVCRMNPDNETMSVSSASISRPPSAGIALGASRKNRPLCHETIRWRSDVPLTEGQLRSKRDEFWDTAPAFDGRKEIWDALRAATNAAEGLDFQMAQAILDGANVSVPNGYLTECYDELGTQYKVPIYCLSYPINIVKEENGRDSPAEYSEPVDGGTEIFLKLRISSSMTDVKLPVYSKDTVGQCKKKLQATEGVDACCQRWFYSGKLLGDKVPIDECSIHQGYVVQVIINTEHYNHDNSTSTVHMSLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URB3</accession>
    <name>A0A6I8URB3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (uracil-O(2)-)-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.211</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4802697</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>uridine(44) in tRNA(Ser) + S-adenosyl-L-methionine = 2'-O-methyluridine(44) in tRNA(Ser) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10339"/>
            <dbReference type="Rhea" id="RHEA-COMP:10340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.211"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRM44 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.211" evidence="9"/>
    <dbReference type="RefSeq" id="XP_001359564.3">
        <property type="nucleotide sequence ID" value="XM_001359527.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URB3"/>
    <dbReference type="FunCoup" id="A0A6I8URB3">
        <property type="interactions" value="1561"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802697"/>
    <dbReference type="InParanoid" id="A0A6I8URB3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141101">
        <property type="term" value="F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011671">
        <property type="entry name" value="tRNA_uracil_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210:SF0">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07757">
        <property type="entry name" value="AdoMet_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359564.3"/>
        </source>
    </evidence>
    <sequence length="525" mass="60733" checksum="C32C3AECDC03AD89" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URX8</accession>
    <name>A0A6I8URX8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribosomal protein S6 kinase delta-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4803187</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359970.2">
        <property type="nucleotide sequence ID" value="XM_001359933.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URX8"/>
    <dbReference type="FunCoup" id="A0A6I8URX8">
        <property type="interactions" value="1378"/>
    </dbReference>
    <dbReference type="GeneID" id="4803187"/>
    <dbReference type="KEGG" id="dpo:4803187"/>
    <dbReference type="InParanoid" id="A0A6I8URX8"/>
    <dbReference type="OMA" id="TFRSDWW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080138">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02677">
        <property type="entry name" value="MIT_SNX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06881">
        <property type="entry name" value="PX_SNX15_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051866">
        <property type="entry name" value="Intracell_Sig-Traffick_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508:SF8">
        <property type="entry name" value="LD24550P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="333"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="189"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359970.2"/>
        </source>
    </evidence>
    <sequence length="684" mass="76855" checksum="2A6FB7BF967C0B8B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US16</accession>
    <name>A0A6I8US16_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Endoplasmic reticulum resident protein 44</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4802950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359763.3">
        <property type="nucleotide sequence ID" value="XM_001359726.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US16"/>
    <dbReference type="FunCoup" id="A0A6I8US16">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802950"/>
    <dbReference type="InParanoid" id="A0A6I8US16"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02996">
        <property type="entry name" value="PDI_a_ERp44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03072">
        <property type="entry name" value="PDI_b'_ERp44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03070">
        <property type="entry name" value="PDI_b_ERp44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052643">
        <property type="entry name" value="ERP44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041870">
        <property type="entry name" value="ERp44_PDI_b_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041862">
        <property type="entry name" value="ERp44_PDI_b_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46295">
        <property type="entry name" value="ENDOPLASMIC RETICULUM RESIDENT PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46295:SF1">
        <property type="entry name" value="ENDOPLASMIC RETICULUM RESIDENT PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026110914" evidence="1">
        <location>
            <begin position="27"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="16"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359763.3"/>
        </source>
    </evidence>
    <sequence length="411" mass="47164" checksum="525188EEEE1E3F65" modified="2020-08-12" version="1">MAFKNLSGCWTILLILGCHHLAPIAGNSVTVGQHNIDQILESTELVLLNFYTDWCPFSIRLAPIFEEAARQINNTFWESGRVTIGKVNCDKEENVALKFEIIKYPTLKILRNGMVVNSEYRGQRSVEAFYQFVAQELADPIKEFRTLEELHDINDDTGLLIGYFMEKNSTEYDNFRKVANFLKKECQFLAGFGEVTEGLRPDGQNLIIFRADSTLSSHREYYSEYSGNLSSFDDLMAWTDDKCIQLVREITFNNAEEITEEGLPFVMLFYDKNNLQHVQEFKTVVETKLSNSSLVNYLTADGQLFQYPLMHLGKSVADLPVIAIDSFRHMYVFPRYEDIHKPGVLQAFIDSLFNGKLHHQLHYGPEPSTAVWENDTSKADTVPAVVPESQFKQLLPSPHRYTLLNRTRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USD7</accession>
    <name>A0A6I8USD7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD-dependent oxidoreductase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4803429</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). Involved in mid-late stages of complex I assembly.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360144.1">
        <property type="nucleotide sequence ID" value="XM_001360107.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USD7"/>
    <dbReference type="FunCoup" id="A0A6I8USD7">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="GeneID" id="4803429"/>
    <dbReference type="KEGG" id="dpo:4803429"/>
    <dbReference type="InParanoid" id="A0A6I8USD7"/>
    <dbReference type="OMA" id="PDHNALI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077099">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000026">
        <property type="entry name" value="FAD-dependent oxidoreductase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF287">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="4">
        <location>
            <begin position="34"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00039785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360144.1"/>
        </source>
    </evidence>
    <sequence length="441" mass="47937" checksum="35EEE1078C4F8E04" modified="2020-08-12" version="1">MLPLGRVLRAVGSLQQFRCLCSKAYGDALPSSCDVLIIGGGGMGASSAFWLTSKARQRGKKLNVLVVERDHGYSKASTVLSVGGVRQQFSLAENIQMGLYGHDFIMNSQKHLGDVDLCFQPHGYLIMATSQGAEILTRNSKLQNELGARNKLLGPEALRKQFPWLCTDKIELGCYGIDKEGWFDPWALLMGFKKQARSFGAKFANGEVVGFEWNAAGALSGAVVDAGDGTQHTVKFDTCVLAAGAHSGQVASLANIGSTQAKEASLRVPLPVEPRKRYVYVFSTQGDNGPGLATPLTVDPDGTYFRRDGLGGNFLCGRSPCDDEEPDCDTLDVDHGYFDTDIWPTLANRVPCFEAVKVQSSWAGYYEHNTFDANGIIGRHPHYSNLFIAAGFSGHGIQQTPAVGRAISELILEGRLTTLDLSRMSFDRIVKQQPMFEVNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USM1</accession>
    <name>A0A6I8USM1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA mismatch repair protein Mlh1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Mlh1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360282.2">
        <property type="nucleotide sequence ID" value="XM_001360245.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USM1"/>
    <dbReference type="FunCoup" id="A0A6I8USM1">
        <property type="interactions" value="1289"/>
    </dbReference>
    <dbReference type="GeneID" id="4803575"/>
    <dbReference type="KEGG" id="dpo:4803575"/>
    <dbReference type="InParanoid" id="A0A6I8USM1"/>
    <dbReference type="OMA" id="ANYHVKK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071082">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03483">
        <property type="entry name" value="MutL_Trans_MLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000014">
        <property type="entry name" value="DNA mismatch repair protein Mlh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000109">
        <property type="entry name" value="Related to MLH1-DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032189">
        <property type="entry name" value="Mlh1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF12">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16413">
        <property type="entry name" value="Mlh1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="7">
        <location>
            <begin position="218"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360282.2"/>
        </source>
    </evidence>
    <sequence length="675" mass="76749" checksum="B5F1EC6D9AE3F6D1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT24</accession>
    <name>A0A6I8UT24_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Khc</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Oligomer composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360474.3">
        <property type="nucleotide sequence ID" value="XM_001360437.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT24"/>
    <dbReference type="FunCoup" id="A0A6I8UT24">
        <property type="interactions" value="818"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803819"/>
    <dbReference type="InParanoid" id="A0A6I8UT24"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098957">
        <property type="term" value="P:anterograde axonal transport of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007292">
        <property type="term" value="P:female gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048489">
        <property type="term" value="P:synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23649">
        <property type="entry name" value="Khc_CBD_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01369">
        <property type="entry name" value="KISc_KHC_KIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000067">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059182">
        <property type="entry name" value="Khc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="12"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="434"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="618"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="854"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001360474.3"/>
        </source>
    </evidence>
    <sequence length="972" mass="110186" checksum="DDC96E4EF2D8BB94" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT39</accession>
    <name>A0A6I8UT39_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General odorant-binding protein 68</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Obp58b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360495.2">
        <property type="nucleotide sequence ID" value="XM_001360458.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT39"/>
    <dbReference type="FunCoup" id="A0A6I8UT39">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803842"/>
    <dbReference type="InParanoid" id="A0A6I8UT39"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054577">
        <property type="entry name" value="OBP47-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052295">
        <property type="entry name" value="Odorant-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066:SF15">
        <property type="entry name" value="GH25962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066">
        <property type="entry name" value="ODORANT-BINDING PROTEIN 59A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22651">
        <property type="entry name" value="OBP47_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026271328" evidence="4">
        <location>
            <begin position="22"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="OBP47-like" evidence="5">
        <location>
            <begin position="69"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360495.2"/>
        </source>
    </evidence>
    <sequence length="204" mass="23322" checksum="334B778C96F50154" modified="2020-08-12" version="1">MVRATFVICLAVAQLLGRLSAIRVHCRHVERIHEDHIHYCCKHPDGHDEVTEMCAKQTSFRLPSTEEEAMEDVTVDQAMVGTCWGKCVFDQYKLLDSNDTLDMIAVHTHYKKYHKTDPEYETEMLNAFQKCHSNTEDAASQFLSLPIVRAFGTGKFCKPASSVILSCVIYNFFHNCPQSRWSNSTECEETRAFAKKCKDVLTTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTF2</accession>
    <name>A0A6I8UTF2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PX domain-containing protein kinase-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Modulator of Na,K-ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4804023</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360653.2">
        <property type="nucleotide sequence ID" value="XM_001360616.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTF2"/>
    <dbReference type="FunCoup" id="A0A6I8UTF2">
        <property type="interactions" value="1237"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804023"/>
    <dbReference type="InParanoid" id="A0A6I8UTF2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043271">
        <property type="term" value="P:negative regulation of monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006622">
        <property type="term" value="P:protein targeting to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06871">
        <property type="entry name" value="PX_MONaKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000010">
        <property type="entry name" value="PX domain-containing protein kinase-like protein isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000830">
        <property type="entry name" value="PX domain-containing serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037903">
        <property type="entry name" value="MONaKA_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF40">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="14"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="185"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="13">
        <location>
            <begin position="637"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="489"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="545"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="500"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="598"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="622"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001360653.2"/>
        </source>
    </evidence>
    <sequence length="660" mass="73078" checksum="005EC2DEF999F3A6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTR2</accession>
    <name>A0A6I8UTR2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360744.1">
        <property type="nucleotide sequence ID" value="XM_001360707.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTR2"/>
    <dbReference type="GeneID" id="4804138"/>
    <dbReference type="KEGG" id="dpo:4804138"/>
    <dbReference type="InParanoid" id="A0A6I8UTR2"/>
    <dbReference type="OMA" id="CHAKPIY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071886">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026317926" evidence="1">
        <location>
            <begin position="27"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360744.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15698" checksum="C08E0B2E842AF019" modified="2020-08-12" version="1">MDLKQLYTWACLLLSIIACKLSQCSAKPTYYDLDSYENYDADRYDTDRYDTGNSYALSYGKPLTENRLKKLNPGYYYVDDGYIAPVSTGTRPYTRRQDANQQVGDLPSNVRFTPLVRVRETRTKRKKLFVPNFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU08</accession>
    <name>A0A6I8UU08_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">28S ribosomal protein S17, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mRpS17</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360909.1">
        <property type="nucleotide sequence ID" value="XM_001360872.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU08"/>
    <dbReference type="FunCoup" id="A0A6I8UU08">
        <property type="interactions" value="1247"/>
    </dbReference>
    <dbReference type="GeneID" id="4804334"/>
    <dbReference type="KEGG" id="dpo:4804334"/>
    <dbReference type="InParanoid" id="A0A6I8UU08"/>
    <dbReference type="OMA" id="TVHAKWI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078119">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000268">
        <property type="entry name" value="Mitochondrial ribosomal protein S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039193">
        <property type="entry name" value="Ribosomal_uS17m_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088">
        <property type="entry name" value="28S RIBOSOMAL PROTEIN S17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360909.1"/>
        </source>
    </evidence>
    <sequence length="155" mass="17991" checksum="8AE71F09E730DFB6" modified="2020-08-12" version="1">MSRRGLLLMGQCMPCIKQNASKIRIRRMELDKNLNMYFKKDDFYFAHDPQKVCKTGDVVLIRELPERMTRLITHAVEKVVYPLGDITDPITGKKVVVGKYREDIEMANELFGKSKKSFDYDKAPPRGRLEGTKDFTHGETYIKYHEDGKDQPFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUL3</accession>
    <name>A0A6I8UUL3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361159.2">
        <property type="nucleotide sequence ID" value="XM_001361122.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUL3"/>
    <dbReference type="GeneID" id="4804627"/>
    <dbReference type="KEGG" id="dpo:4804627"/>
    <dbReference type="InParanoid" id="A0A6I8UUL3"/>
    <dbReference type="OMA" id="RPQACLG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074148">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361159.2"/>
        </source>
    </evidence>
    <sequence length="169" mass="19511" checksum="3A398FE56B77059C" modified="2020-08-12" version="1">MKYYPDGEAWFYVDRPSCMTSYQKYPKWHSWKLQQRSINRVMHYWRDTDGVKMAEVKRADLYFTNWPKSRIRPQACLGMLYATGFRFIRLSKAPPAGFKCAPLPINLAAARIVKVNLAKKRKADKKAAKKAKAEAKKAKQEAKLANKGKKGKPGELKPKVIKTYENVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUU2</accession>
    <name>A0A6I8UUU2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein orthopedia</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">otp</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360970.3">
        <property type="nucleotide sequence ID" value="XM_001360933.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUU2"/>
    <dbReference type="GeneID" id="4804400"/>
    <dbReference type="KEGG" id="dpo:4804400"/>
    <dbReference type="InParanoid" id="A0A6I8UUU2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070082">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000400">
        <property type="entry name" value="Orthopedia, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051895">
        <property type="entry name" value="OTP_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46770">
        <property type="entry name" value="HOMEOBOX PROTEIN ORTHOPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46770:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN ORTHOPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="104"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="10">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="106"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="278"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001360970.3"/>
        </source>
    </evidence>
    <sequence length="415" mass="43878" checksum="C6E65845F0AEA115" modified="2020-08-12" version="1">MLNNLGANVLGPKDCDLPKAALTALHAGVNSFGQLPGAPNLADLGGSGGGGVGLPVGGGVSRLHISGGLCDNANNSGNNSSGGGGGGGSGNTNMQQQDQLLDKNKQKRHRTRFTPAQLNELERCFSKTHYPDIFMREEIAMRIGLTESRVQVWFQNRRAKWKKRKKTTNVFRTPGALLPSHGLPPFGANITNIAMGDGLCGTGMFGGDRWSVGVNPMTAGFGQLNQSSPLSSSLNSGLNSGINMGSALGAGSYQHYGLNALGDSMMYQHSVGGVACGPSGSPSATTPPNMNSCSSVTPPPISTQPNASQNELSGESMPLHQQQQQQTHQQQQQQTHQHSQMAPPTPSQQQQQEGLHCHQSMQSPTDGATDEDVWRGHSIAALRRRASELNATAIPSYLHHAAAAHNNYEHHNSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUX0</accession>
    <name>A0A6I8UUX0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ras-specific guanine nucleotide-releasing factor RalGPS1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RalGPS</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361277.2">
        <property type="nucleotide sequence ID" value="XM_001361240.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUX0"/>
    <dbReference type="FunCoup" id="A0A6I8UUX0">
        <property type="interactions" value="691"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804766"/>
    <dbReference type="InParanoid" id="A0A6I8UUX0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13310">
        <property type="entry name" value="PH_RalGPS1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000397">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF368">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="168"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="576"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361277.2"/>
        </source>
    </evidence>
    <sequence length="703" mass="79156" checksum="EB2E02A4B459C84B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVH8</accession>
    <name>A0A6I8UVH8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">General odorant-binding protein 56h</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Obp56h</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361442.1">
        <property type="nucleotide sequence ID" value="XM_001361405.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVH8"/>
    <dbReference type="SMR" id="A0A6I8UVH8"/>
    <dbReference type="FunCoup" id="A0A6I8UVH8">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="4804966"/>
    <dbReference type="KEGG" id="dpo:4804966"/>
    <dbReference type="InParanoid" id="A0A6I8UVH8"/>
    <dbReference type="OMA" id="VPQMKDK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072636">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857:SF43">
        <property type="entry name" value="GEO07291P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857">
        <property type="entry name" value="ODORANT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00708">
        <property type="entry name" value="PhBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026089775" evidence="6">
        <location>
            <begin position="20"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361442.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="14946" checksum="DC1D2C453B854141" modified="2020-08-12" version="1">MKSTLFCAALAMFLAVVQCDPDFRQLMQECMQTTQVTEADLKDFMASGMQGTAKENLKCYTKCLMEKQGHLVNGQFNAQAMLDSLKNVPQIKDKMDEISSGVNACKDMKGTNDCDTAFKITMCLKEHKLIPGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW91</accession>
    <name>A0A6I8UW91_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl-CoA hydratase domain-containing protein 3, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4805387</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play a role in fatty acid biosynthesis and insulin sensitivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361796.4">
        <property type="nucleotide sequence ID" value="XM_001361759.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW91"/>
    <dbReference type="FunCoup" id="A0A6I8UW91">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805387"/>
    <dbReference type="InParanoid" id="A0A6I8UW91"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052377">
        <property type="entry name" value="Mitochondrial_ECH-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43602">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43602:SF1">
        <property type="entry name" value="ENOYL-COA HYDRATASE DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361796.4"/>
        </source>
    </evidence>
    <sequence length="285" mass="31160" checksum="4BF8E20754347BF5" modified="2020-08-12" version="1">MFRKLQIKTVRYATGAYGQVFRLVSSQSQAPELVLVKEANGVREITLNYPRTLNSLSMDMMCALQNALVKDKDDVKLRCVVLSANGKIWSAGHNLKELHGNPDIQRSVFQKLTDIIRDIQLLPVPVIGKVNGYAAAAGCQLAVSCDIVVCSKTSKFSTPGAGVGVFCSTPGVAIARVMSRPKSAYMLMTGLPVTGEEAFISGMVSRVVQAEDLDREIDEITCAINAKSRAVISLGKEFYYKQLAMPLNEAYPAAGQKMEENLQLSDCQEGIASFIEKRAPHWTHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWA7</accession>
    <name>A0A6I8UWA7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Cht4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001361912.3">
        <property type="nucleotide sequence ID" value="XM_001361875.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWA7"/>
    <dbReference type="FunCoup" id="A0A6I8UWA7">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805523"/>
    <dbReference type="InParanoid" id="A0A6I8UWA7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
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        <property type="entry name" value="Chitinase"/>
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        <property type="entry name" value="Glycosidases"/>
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        <property type="entry name" value="Chitinase_II/V-like_cat"/>
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        <property type="entry name" value="Chitinase_insertion_sf"/>
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        <property type="entry name" value="GH"/>
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        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
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        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
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        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CHITINASE 4-RELATED"/>
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        <property type="entry name" value="CBM_14"/>
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        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
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        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GH18_1"/>
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        <property type="entry name" value="GH18_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="4" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="22"/>
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            <begin position="376"/>
            <end position="409"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="13">
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            <end position="387"/>
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            <end position="409"/>
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        <source>
            <dbReference type="RefSeq" id="XP_001361912.3"/>
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    <sequence length="465" mass="50568" checksum="1A504883F49AD010" modified="2020-08-12" version="1">MKLYSFWAFAVLCLCLGQVATSEKLVNCYWGTWANYRPGDGKFTPSDIDPFLCTHISYTFFGIGDGGEFKSLDTWLDMDDGLGFISQTIALKQRNPKLKILAVVGGWNEGSIKYSEMAADPGKRATFISTSLAFIQQYGFDGLDLDWEYPGQRGGNEEADRVNFVTLLREIKETYDRYGLELGIAVGASEKSAKISYDIPAISQHLTFINVMTYDFHMALDGYLGLNAPLPEVTESIDYWLAQGAPAEKLILGIGFYGHSYQMADASQNWPGAACIGPGSAGVYTRENGFMGYHEICLNNWQTVFDQENGAPYAFQGDQWIGYDNPESIEMKMKLVESRNLGGAMMWSIETDDFRGLCGESYPLLKTMNRALGKEVSGGGGGGGGGNVTPAPTPGPTQGGGGGGGGSGSPAGDCSSDGYFLHSSDCSRYYQCVGGLRYDFECTPGLHFDVSINTCNWPEEANCPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWE5</accession>
    <name>A0A6I8UWE5_DROPS</name>
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        <submittedName>
            <fullName evidence="14">Zinc finger protein 665</fullName>
        </submittedName>
    </protein>
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        <name evidence="14" type="primary">LOC4805573</name>
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        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
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        <property type="entry name" value="Znf_AD"/>
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        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
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    </dbReference>
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        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
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    <dbReference type="Pfam" id="PF00096">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="12">
        <location>
            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
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        <location>
            <begin position="262"/>
            <end position="290"/>
        </location>
    </feature>
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        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
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        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
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        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="186"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001361956.3"/>
        </source>
    </evidence>
    <sequence length="419" mass="47824" checksum="0CB2ECB56221B1BE" modified="2020-08-12" version="1">MFSLLKQPLICRCCLLEQPPLYRHIHDDHLLAELKALAPTLRLDAGDGLTDVICDLCLRRLHDARNFQRECEHSELVLRTRHEQWQHTVAAGDALALDDVLECLEREVGSLEDTMMPVLQGKPVAYVAPLMETVDFDTLEFQESLSSPYEAPILWDADAGPLNTPLHQPETDSGTLLQLETETAEHLEAPQAVPEPEMPLAKPKPPKAMPKRKRPQSSPAAVRSPDSPRSLHPCTECEKKFTRNFQLKLHMIAVHGMGELLYQCEECHKSFASRHSLRYHVKSVHSTERPFSCDHCERKFVLRTQLSSHLRTHTGESKPRIFACPKCTKTWPTKSDLRTHMRSHDPHMERPFKCDRCEKSFFTRGHLSSHILVHTGEKPYGCDYCDKCYQSVGNLNNHMVRQHADIIEAQLEMEAAIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXI8</accession>
    <name>A0A6I8UXI8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein argonaute-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6903043</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132767.2">
        <property type="nucleotide sequence ID" value="XM_002132731.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXI8"/>
    <dbReference type="KEGG" id="dpo:6903043"/>
    <dbReference type="InParanoid" id="A0A6I8UXI8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="242"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="517"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002132767.2"/>
        </source>
    </evidence>
    <sequence length="845" mass="95368" checksum="BBA33357FD705C24" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXK7</accession>
    <name>A0A6I8UXK7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ttm3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the TIM23 complex at least composed of Tim23, Tim17 (Tim17a1, Tim17a2 or Tim17b1) and a Tim50.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Mitochondrion inner membrane</location>
            <topology evidence="2 14">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TIM50 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132271.2">
        <property type="nucleotide sequence ID" value="XM_002132235.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXK7"/>
    <dbReference type="FunCoup" id="A0A6I8UXK7">
        <property type="interactions" value="1242"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903392"/>
    <dbReference type="InParanoid" id="A0A6I8UXK7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXK7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000019">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10 14" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="16">
        <location>
            <begin position="143"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002132271.2"/>
        </source>
    </evidence>
    <sequence length="349" mass="40951" checksum="3B18781F0D4197F2" modified="2020-08-12" version="1">MAGYGGLCLRGLRLWMRTSHIYRGCSGKTPVPKTPVEEKPHRKLQSTSSSNASEDPYTFRRIRRISVYSLVVGSLVGAVLWAVYELGKPEMDQHGVPIEDQFSGLPWVQEFLERMWHSLQYYQKMLEEPLPVKLLPDTLPPPYIQPPYTLVLEINDILVHLDWTYQTGWRFKKRPGVDFFLRKCSKYFEIVVYTSEQGMIAFPLMDALDPYGYISYRLVRGDTELVEGQHVKKLQNLNRNLSRVVVVDWDRNATPQHPENTFVMTRWLGNDDDVQLFDLTAFLELLASKNINDVREILQYYNKFEDPIAQFRENQRRLLEREDKTTVKTVQTQRHWTTRLLGRYRGTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY08</accession>
    <name>A0A6I8UY08_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SNRNP25 ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132976.3">
        <property type="nucleotide sequence ID" value="XM_002132940.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY08"/>
    <dbReference type="KEGG" id="dpo:6903277"/>
    <dbReference type="InParanoid" id="A0A6I8UY08"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UY08">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132976.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="44835" checksum="245CF1786E5A78A8" modified="2020-08-12" version="1">MDRGEYRKDRSTDRREHSLQRRDRSSSHGRERSSHRKDRTSHKRKHSSRKKERSPHTASDRRESRDRSSHERKRSSHRKEGAATSRRERSPHRKVYLHRKEHSSPHRRERSPHRAKRFPPEEHLRTPETNVLTYKKLQQMPVNLRKKAIREARSRNEEILSNRKGKKKKTKSWITARRQCNAAECSEHNNLVDSTEQELRQILQGFDVLSDIPCDCTATELLGEIAIAEGNATTIFVERDGLSTLTIVLHQPEPTIAQLKKAIAVISRAEHKRRQRERHEESLRRRGLADGGPEDPQRQQQVAGGAMPSSTGELVYSALRNGHDHRAFVSWHFLWRCFGLFNVETNQPIDDRSNGRATLTELGIENAAALKFVHRVKYFGCQRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYM2</accession>
    <name>A0A6I8UYM2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA/RNA non-specific endonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6902048</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133582.2">
        <property type="nucleotide sequence ID" value="XM_002133546.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYM2"/>
    <dbReference type="KEGG" id="dpo:6902048"/>
    <dbReference type="InParanoid" id="A0A6I8UYM2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.570.10:FF:000007">
        <property type="entry name" value="Alkaline nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF19">
        <property type="entry name" value="NUCLEASE EXOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026328791" description="DNA/RNA non-specific endonuclease domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="8">
        <location>
            <begin position="183"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="7">
        <location>
            <begin position="189"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002133582.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="49826" checksum="E66482D030E6A413" modified="2020-08-12" version="1">MNLSARLLFIAVIAASVSLREIRAREAITPSPEVGQIIKDLDGLHIDGTGTAWRQGASCKVDVQRDLPPPRSFQPLYLRPNTSLYWLPNANGQLEIPSGHPIELYCRGSFSEITEYRESIQPKCLQGTTFELNGSKLEFRKFTCSQSMTYTVEKLESSCGSSPSPADQSHLFRVGYDIGRGRFVETMNLCHDPQTVRTHYAVHQLTPASQHFQQKVKRPKFTTAGHFEGYDMNNIYSFKNQQSLLGGLVNKKENQFLSRGHLVAKADLIYASQQRSSFNYMNTAPQWQLFNAGSWQNLEEATRRFVANAGIEVEVYTGTYGQMPFPNASAIVHLATDTNKRQVLEVPRLFYRVLIDKVNRSRGIALVGVNSPNAILSEIKDPNVICDPIDPIEEKVPWLGWVAKANNKSRQLMQGGYLYVCSVSDLAGAVKELPNHLGDVSELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ02</accession>
    <name>A0A6I8UZ02_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type Cu(+) transporter</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ATP7</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002133748.2">
        <property type="nucleotide sequence ID" value="XM_002133712.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ02"/>
    <dbReference type="FunCoup" id="A0A6I8UZ02">
        <property type="interactions" value="1396"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902221"/>
    <dbReference type="InParanoid" id="A0A6I8UZ02"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ02">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060003">
        <property type="term" value="P:copper ion export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000150">
        <property type="entry name" value="ATP7, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000049">
        <property type="entry name" value="copper-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000031">
        <property type="entry name" value="Probable copper-transporting ATPase HMA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-0187">Copper transport</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="459"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="651"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="696"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1103"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1130"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="30"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="100"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="219"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="295"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="170"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1230"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_002133748.2"/>
        </source>
    </evidence>
    <sequence length="1271" mass="137560" checksum="E9174E6876367683" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZN5</accession>
    <name>A0A6I8UZN5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Probable cytochrome P450 28a5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Cyp28a5</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134369.2">
        <property type="nucleotide sequence ID" value="XM_002134333.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZN5"/>
    <dbReference type="FunCoup" id="A0A6I8UZN5">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901699"/>
    <dbReference type="InParanoid" id="A0A6I8UZN5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZN5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF84">
        <property type="entry name" value="CYTOCHROME P450 28A5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002134369.2"/>
        </source>
    </evidence>
    <sequence length="505" mass="57812" checksum="FC4F46B1600D1FDA" modified="2020-08-12" version="1">MILITLALGLATVGLLYLLMTWNFDHWRKRRVPGPKPKIPTGNYPHMYTMKRHAIYDLNDIYVKYKNKYDAVGIFGGRVPQLLVISPELARRVFVSDFKHFHDNEVSNLIDEKSDFIFANNPFSLTGDKWKQRRADVTPGLTMSRIKTVYPVTNKVCQKLSEWVDKQIRLGAPEGINAKDMSLCFTSEMVTDCVLGLSAESFSDNPTPIMGHIKDLFSQSWSFLIYFVIISTFPSLSHLIKLRFVPPKVERFFIELMENAVKARRAQLVAGKQFERTDFLDYILQMAEKRSLDTRQLLAHTMTFLLDGFETTATVLAHMLLLLGREPEAQKRLREEILAHVKDGIVSFEKLNELPFLDACVQETIRIFPPAFMSNKLCTEAIELPNKDGPNFTVEVGTTIVIPHYCFMLDEDYFPDSQSFQPDRFMEPDAAKKFRERGTFMGFGDGPRVCIGMRFALAQIKAAVVELITKYNVKVNPKTRKDNLYDPMSFITSLQGGIWLDLEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1C9</accession>
    <name>A0A6I8V1C9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome activator PA28 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135449.2">
        <property type="nucleotide sequence ID" value="XM_002135413.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1C9"/>
    <dbReference type="KEGG" id="dpo:6900091"/>
    <dbReference type="InParanoid" id="A0A6I8V1C9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008537">
        <property type="term" value="C:proteasome activator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.180">
        <property type="entry name" value="Proteasome activator pa28, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003186">
        <property type="entry name" value="PA28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036997">
        <property type="entry name" value="PA28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036252">
        <property type="entry name" value="Proteasome_activ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02252">
        <property type="entry name" value="PA28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47216">
        <property type="entry name" value="Proteasome activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome activator PA28 C-terminal" evidence="1">
        <location>
            <begin position="79"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135449.2"/>
        </source>
    </evidence>
    <sequence length="209" mass="24089" checksum="6959D034100617AA" modified="2020-08-12" version="1">MAANGAEQGLLLKVMLVDSLFESLDLVITQNFPSTIFRLNELLAAPMFNNPMFADEPPGCRLPPELKWAQTCRLQPVVALTAETTRVINCLFGYCSIVEELVEGMKREVEHRGYADVRTHNEILSLLRAIQSATPLYYELMNIYNRTRQRLTDLMAESPGHKKHYLCRIYHTDKTEYQALCNIVWRVRNDFSAIQNMVLEHWDDLKPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1H6</accession>
    <name>A0A6I8V1H6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6904092</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002136427.3">
        <property type="nucleotide sequence ID" value="XM_002136391.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1H6"/>
    <dbReference type="FunCoup" id="A0A6I8V1H6">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6904092"/>
    <dbReference type="InParanoid" id="A0A6I8V1H6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000057">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026276175" description="Lipase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002136427.3"/>
        </source>
    </evidence>
    <sequence length="392" mass="44086" checksum="6400F349F77EF039" modified="2020-08-12" version="1">MAQILLRWIVLVVNILGGVALKDTGDYIRMHNYPVEKHTAVTPDGYILGLFRIPNSPRRPSTSGPKPAVLFVHGMTCSSDYWVIIGPEQGLPFLLADEGYDVWLINSRGNIYSRKHLTISPNNKDFWQFDWHEIGIYDTTTSIDFILSMTGQTAVHYVGHSQGATSFLAMLSMRPEYNIKVKTSHLLGPVAFSGKMPSKLFKVINNFYLQLGDMELKYNTPFWSRIFSSLCTVLLLRHILCRNVAFLISGGSSRHLNMTLLPAMAATASAGISTRQIKHYVQLIDSGRFALFDFGKRDNLATYGTTDPPDYPLNEVNPLSPIDFYYSENDGMAAVEDVLLTIHSLPNARGHRHQFSDWGHIDYVFGNNLKFYVNNDIVNIANAFESHLNEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2C9</accession>
    <name>A0A6I8V2C9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein beat-VII</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">beat-VII</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137329.3">
        <property type="nucleotide sequence ID" value="XM_002137293.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2C9"/>
    <dbReference type="FunCoup" id="A0A6I8V2C9">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="GeneID" id="6897149"/>
    <dbReference type="KEGG" id="dpo:6897149"/>
    <dbReference type="InParanoid" id="A0A6I8V2C9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0262661">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002321">
        <property type="entry name" value="GG12177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261">
        <property type="entry name" value="BEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261:SF3">
        <property type="entry name" value="BEATEN PATH VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026186779" evidence="2">
        <location>
            <begin position="22"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="18"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002137329.3"/>
        </source>
    </evidence>
    <sequence length="521" mass="58391" checksum="2DED311F1E3B4060" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2Q4</accession>
    <name>A0A6I8V2Q4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cell death abnormality protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6897184</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137364.2">
        <property type="nucleotide sequence ID" value="XM_002137328.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2Q4"/>
    <dbReference type="KEGG" id="dpo:6897184"/>
    <dbReference type="InParanoid" id="A0A6I8V2Q4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006149">
        <property type="entry name" value="EB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39069">
        <property type="entry name" value="ECDYSONE-INDUCIBLE GENE E1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39069:SF8">
        <property type="entry name" value="FI17111P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01683">
        <property type="entry name" value="EB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026006138" evidence="3">
        <location>
            <begin position="24"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="687"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="568"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002137364.2"/>
        </source>
    </evidence>
    <sequence length="712" mass="78481" checksum="9C0E475A1EC595E7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3N6</accession>
    <name>A0A6I8V3N6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">PKD</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28">MV-25-SWS-2005</strain>
            <tissue evidence="28">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="19">Activated by DAG and phorbol esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="19"/>
    <dbReference type="RefSeq" id="XP_002137390.3">
        <property type="nucleotide sequence ID" value="XM_002137354.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3N6"/>
    <dbReference type="FunCoup" id="A0A6I8V3N6">
        <property type="interactions" value="548"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897218"/>
    <dbReference type="InParanoid" id="A0A6I8V3N6"/>
    <dbReference type="OMA" id="PNNCSHD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248554">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V3N6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20795">
        <property type="entry name" value="C1_PKD_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20796">
        <property type="entry name" value="C1_PKD_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14082">
        <property type="entry name" value="STKc_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000151">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000137">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000021">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000068">
        <property type="entry name" value="Serine/threonine-protein kinase D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015727">
        <property type="entry name" value="Protein_Kinase_C_mu-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25525">
        <property type="entry name" value="Ubiquitin_PRKD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000552">
        <property type="entry name" value="PKC_mu_nu_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19 28" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="104"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="223"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="24">
        <location>
            <begin position="415"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="555"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="159"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="304"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="359"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="678"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="561"/>
            <end position="569"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 22">
        <location>
            <position position="584"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_002137390.3"/>
        </source>
    </evidence>
    <sequence length="848" mass="94729" checksum="A7ABEBB4CAEBBD8E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4E4</accession>
    <name>A0A6I8V4E4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Aquaporin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Eglp1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137867.2">
        <property type="nucleotide sequence ID" value="XM_002137831.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4E4"/>
    <dbReference type="FunCoup" id="A0A6I8V4E4">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897762"/>
    <dbReference type="InParanoid" id="A0A6I8V4E4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF270">
        <property type="entry name" value="ENTOMOGLYCEROPORIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002137867.2"/>
        </source>
    </evidence>
    <sequence length="245" mass="26427" checksum="0974ACFDFBC77C41" modified="2020-08-12" version="1">MILDRTTLSRALGEFAATALLMIVGCMGVAMTFPEETGFLVGSLQYGLTVGIVVHVFGCVSGAHTNPSVSISCCILGHIAFDMMLVYVACQLAGGLAGYYFLMAMLPKDVIDQSYPAVCLQEPMADLSDIQILAVEFTLTSVLVLGWCAQWDVRNGSCLDSAPIRMGLLVTACGFAGNQLTGASMNPAKTLVPLLFHDHQSTFYPQLGGQTLAAVLVPFIWRYALTSNAKPMKTTSRRPKRTRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5Q1</accession>
    <name>A0A6I8V5Q1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC13036432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736408.3">
        <property type="nucleotide sequence ID" value="XM_003736360.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5Q1"/>
    <dbReference type="KEGG" id="dpo:13036432"/>
    <dbReference type="InParanoid" id="A0A6I8V5Q1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_003736408.3"/>
        </source>
    </evidence>
    <sequence length="202" mass="22801" checksum="AE5FFF4B88650D1C" modified="2020-08-12" version="1">MWFNMTALDMNMFAMADDVYNLMEIFDEASSFEGRIIKRIENALVICDILVSVLVATIVGRYLFHSCFKKLHRSVKRTSYLPKFIAQIKQFNQEMGVQGSSVKSLDSIQPISQMKPIIHKMSVRGSKAKIWKTDVDHCEQKPKNGQDQTVQAIITGRSSIEGIDDGDEVTMKAPERENFSVKLMGTQEWAANYLATGGRTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VCX5</accession>
    <name>A0A6I8VCX5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="7">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Vha55</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Essential for the proper assembly and activity of V-ATPase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits VhaAC45 and ATP6AP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015038571.1">
        <property type="nucleotide sequence ID" value="XM_015183085.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VCX5"/>
    <dbReference type="SMR" id="A0A6I8VCX5"/>
    <dbReference type="FunCoup" id="A0A6I8VCX5">
        <property type="interactions" value="1816"/>
    </dbReference>
    <dbReference type="GeneID" id="4800524"/>
    <dbReference type="KEGG" id="dpo:4800524"/>
    <dbReference type="InParanoid" id="A0A6I8VCX5"/>
    <dbReference type="OMA" id="EDHPQVM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074512">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="9">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="8">
        <location>
            <begin position="152"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="10">
        <location>
            <begin position="385"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015038571.1"/>
        </source>
    </evidence>
    <sequence length="490" mass="54544" checksum="188F64C89BB9E41A" modified="2020-08-12" version="1">MNAQQAHKEHVLAVSRDFISQPRLTYKTVSGVNGPLVILDEVKFPKFAEIVQLRLADGTIRSGQVLEVSGSKAVVQVFEGTSGIDAKNTLCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPPILAEDFLDIQGQPINPWSRIYPEEMIQTGISAIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKLPGKSVLDDHTDNFAIVFAAMGVNMETARFFKQDFEENGSMENVCLFLNLANDPTIERIITPRLALTAAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPVNVLPSLSRLMKSAIGEGMTRKDHSDVSNQLYACYAIGKDVQAMKAVVGEEALTPDDLLYLEFLTKFEKNFISQGNYENRTVFESLDIGWQLLRIFPKEMLKRIPASILAEFYPRDSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VD00</accession>
    <name>A0A6I8VD00_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein SERAC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4801080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015038658.2">
        <property type="nucleotide sequence ID" value="XM_015183172.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VD00"/>
    <dbReference type="FunCoup" id="A0A6I8VD00">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801080"/>
    <dbReference type="InParanoid" id="A0A6I8VD00"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="321"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="444"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015038658.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="78705" checksum="58BE48272D974B02" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VF35</accession>
    <name>A0A6I8VF35_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FXNA-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC6898437</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015039781.2">
        <property type="nucleotide sequence ID" value="XM_015184295.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VF35"/>
    <dbReference type="InParanoid" id="A0A6I8VF35"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000008">
        <property type="entry name" value="Endoplasmic reticulum metallopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053973">
        <property type="entry name" value="ERMP1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF22">
        <property type="entry name" value="ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22248">
        <property type="entry name" value="ERMP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="480"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="518"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="545"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="614"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="642"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="17">
        <location>
            <begin position="173"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum metallopeptidase 1-like C-terminal" evidence="18">
        <location>
            <begin position="672"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015039781.2"/>
        </source>
    </evidence>
    <sequence length="900" mass="100766" checksum="C3535CED075E883A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFV6</accession>
    <name>A0A6I8VFV6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-tocopherol transfer protein isoform X3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4805121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040451.2">
        <property type="nucleotide sequence ID" value="XM_015184965.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFV6"/>
    <dbReference type="FunCoup" id="A0A6I8VFV6">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VFV6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071962">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
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        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF216">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
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        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="165"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015040451.2"/>
        </source>
    </evidence>
    <sequence length="362" mass="40724" checksum="040411457EA49CB9" modified="2020-08-12" version="1">MPYTIYHNQPEPEPKSAFPCRGGGSRAPTVKVSGSAFKFCDEVGGDKERVAMSDQNSIRSLSPELASKAQKELGEVPDRIEEDIDTLRTWISKQPHLKARQDAQFLIAFLRGCKYSLEKTKLKLDNFYAMRGVVPELYKNRFVGEKQMGILETGCLLRLPQPLGADGPRIHISRYGLYDSKKYSIAEVVQVSTMIGEIQIREDDNAMISGFVEIIDMKGVGAGHIFQFDAVLVKKLAVLGDKALPYRPKGFHFVNAPASAETFMSIAKSLMSEKIRKRFHIHTKLESLYKYVPKECLPAEYGGSNGTIDDVVTTWREKLLSYQNFFEEEVSYGTNEKLRRGDAVNVESLFGIEGSFRKLDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIA1</accession>
    <name>A0A6I8VIA1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901622</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041899.2">
        <property type="nucleotide sequence ID" value="XM_015186413.2"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Blast:Monocarboxylate transporter 13"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
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    <dbReference type="PANTHER" id="PTHR11360:SF8">
        <property type="entry name" value="BCDNA.LD28120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="249"/>
            <end position="266"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="454"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="519"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="542"/>
            <end position="567"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="579"/>
            <end position="601"/>
        </location>
    </feature>
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        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015041899.2"/>
        </source>
    </evidence>
    <sequence length="643" mass="71585" checksum="E7B392B6DF16A41D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJW6</accession>
    <name>A0A6I8VJW6_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="30" type="primary">btl</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="30">MV-25-SWS-2005</strain>
            <tissue evidence="30">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
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        <property type="nucleotide sequence ID" value="XM_015187419.2"/>
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        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532882"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VJW6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:receptor complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005007">
        <property type="term" value="F:fibroblast growth factor receptor activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001667">
        <property type="term" value="P:ameboidal-type cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008347">
        <property type="term" value="P:glial cell migration"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045887">
        <property type="term" value="P:positive regulation of synaptic assembly at neuromuscular junction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090130">
        <property type="term" value="P:tissue migration"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048729">
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000651">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000983">
        <property type="entry name" value="Fibroblast growth factor receptor homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
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        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
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    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
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        <property type="entry name" value="Tyr_kinase_AS"/>
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    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF550">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR HOMOLOG 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
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    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
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            <begin position="1038"/>
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            <begin position="385"/>
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        <location>
            <position position="768"/>
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        <location>
            <position position="888"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <location>
            <position position="889"/>
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        <ligand>
            <name>Mg(2+)</name>
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        <location>
            <position position="902"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    <sequence length="1072" mass="119735" checksum="11F5DF21A06DC4F8" modified="2020-08-12" version="1"> </sequence>
</entry>
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            <taxon>Diptera</taxon>
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                <consortium name="RefSeq"/>
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            <strain evidence="8 9 10">MV-25-SWS-2005</strain>
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        </subcellularLocation>
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        <text evidence="4">Belongs to the synaptopodin family.</text>
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</entry>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="RefSeq"/>
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            <begin position="620"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_002137799.2"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033232291.1"/>
        </source>
    </evidence>
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</entry>
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        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
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        <name evidence="4 5" type="primary">LOC6896990</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033376580.1"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5044634535" evidence="2">
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            <end position="1599"/>
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    </feature>
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        </location>
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        <location>
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            <end position="1463"/>
        </location>
    </feature>
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            <end position="1599"/>
        </location>
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            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
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            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
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            <end position="270"/>
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    </feature>
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            <end position="315"/>
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    </feature>
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        <location>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033232470.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033232471.1"/>
        </source>
    </evidence>
    <sequence length="1599" mass="175064" checksum="09FDDA68211D2097" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPE7</accession>
    <name>A0A6I8VPE7_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
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        </recommendedName>
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        <name evidence="18" type="primary">Ptp99A</name>
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    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
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        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein-tyrosine phosphatase family. Receptor class subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_033377020.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="4"/>
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    <dbReference type="CDD" id="cd14550">
        <property type="entry name" value="R5-PTP-2"/>
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    </dbReference>
    <dbReference type="CDD" id="cd14549">
        <property type="entry name" value="R5-PTPc-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase gamma"/>
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    </dbReference>
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        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="receptor-type tyrosine-protein phosphatase zeta"/>
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        <property type="entry name" value="Tyrosine-protein phosphatase 99A"/>
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        <property type="entry name" value="Tyr_Pase_AS"/>
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        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
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        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
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        <property type="entry name" value="fn3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="558"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="138"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="240"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="340"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="437"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="643"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
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            <begin position="825"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="931"/>
            <end position="1183"/>
        </location>
    </feature>
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        <location>
            <begin position="1257"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1257"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1287"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1315"/>
            <end position="1329"/>
        </location>
    </feature>
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        <location>
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            <end position="1345"/>
        </location>
    </feature>
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        <location>
            <begin position="1347"/>
            <end position="1380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061343"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033232911.1"/>
        </source>
    </evidence>
    <sequence length="1489" mass="168035" checksum="BE33772C95BDCB35" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPN8</accession>
    <name>A0A6I8VPN8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-C2HC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183388</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233040.1">
        <property type="nucleotide sequence ID" value="XM_033377149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPN8"/>
    <dbReference type="KEGG" id="dpo:117183388"/>
    <dbReference type="InParanoid" id="A0A6I8VPN8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="32081" checksum="87C977F0FB5F41A9" modified="2020-08-12" version="1">MAARSGIPFPKYLTVSRLNSRIGLQRVMVEHICNALENICCGPVDFCQKLSNGTVLVKTWAANLSAAKLPQVCFLPNNIDVEVKEHRSLNQGRAVMHSYDLAKHSEAEILQQLQEHNVISVRKITRWRQQPSKKHETGLIILHFDTYPPPSIQLNGKIIRLHPYVPTPMRCRNCLLFGHPTRDCQRPSACLNCGYAKHLNIGEKCSRNQACVNCRTRKVPHSRHHTLDRTCPTFIWEDQIKAICIISRVSRRRAISIYNSQLPRDMSHFSEAARLSYLKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPR9</accession>
    <name>A0A6I8VPR9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC4803183</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359966.3">
        <property type="nucleotide sequence ID" value="XM_001359929.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233075.1">
        <property type="nucleotide sequence ID" value="XM_033377184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPR9"/>
    <dbReference type="KEGG" id="dpo:4803183"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF30">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="2">
        <location>
            <begin position="154"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359966.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033233075.1"/>
        </source>
    </evidence>
    <sequence length="448" mass="50747" checksum="887D3C1EE0D0896A" modified="2020-08-12" version="1">MGITTETATTATGMGAANGGAADVESKQILSLEVFQELFKHVEPDVEIDAFELAQGSDRGDNYTAALYRINLSGKRQSLDGSQHKWTQSVICKVMPESVAQREAYKSDKLFRNEVEFYTTIMPELLKFQASKTGRDSPVFNAIPKCYTARHDLLIMEDLRERGFQMSDRHKGLSIEETQSVLLQVAQLHGLSLAYKFEHPLDFAKLCSLISEGIFCTANTSWYRNYYERLTKNAVKMVSEVLPADSKYVLAMRNFAESSSFFGQMVKIASSESPLSAICHGDCWVNNFLYHYDPEDPQLVLEVALIDFQLIRYSSIALDIANLLYCCTTKEMRDAELKSLLKTYTEELFRWLKILCTELPEHCNTLEKLQKLFAEELKAYGRFALGLAMDIIPISTCSSEDAPDMYLNRNDEPEEEDEGAPTLNFPPNDLCRQKMVEIVKDMVDGDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VR64</accession>
    <name>A0A6I8VR64_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aspartyl/asparaginyl beta-hydroxylase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Asph</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartyl/asparaginyl beta-hydroxylase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233532.1">
        <property type="nucleotide sequence ID" value="XM_033377641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VR64"/>
    <dbReference type="FunCoup" id="A0A6I8VR64">
        <property type="interactions" value="432"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805634"/>
    <dbReference type="InParanoid" id="A0A6I8VR64"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081054">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VR64">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062101">
        <property type="term" value="F:peptidyl-aspartic acid 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007803">
        <property type="entry name" value="Asp/Arg/Pro-Hydrxlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039038">
        <property type="entry name" value="ASPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366:SF29">
        <property type="entry name" value="ASPARTYL BETA-HYDROXYLASE, ISOFORM L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366">
        <property type="entry name" value="ASPARTYL/ASPARAGINYL BETA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05118">
        <property type="entry name" value="Asp_Arg_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartyl/asparaginy/proline hydroxylase" evidence="4">
        <location>
            <begin position="863"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="121"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="395"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="568"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="480"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="518"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="593"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033233532.1"/>
        </source>
    </evidence>
    <sequence length="1029" mass="118187" checksum="F3A8AF7A1629E7FC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSA1</accession>
    <name>A0A6I8VSA1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233554.1">
        <property type="nucleotide sequence ID" value="XM_033377663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VSA1"/>
    <dbReference type="KEGG" id="dpo:117183539"/>
    <dbReference type="InParanoid" id="A0A6I8VSA1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026322270" description="MD-2-related lipid-recognition domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="20230" checksum="7ADEF66D5D515771" modified="2020-08-12" version="1">MNYKIISFGMVLFAIFLTGSEAPFVKMTNAKCPSYNKSWVEVHYCRLKAYSRNKTSLNINATFLQPANNIFLRLKLMKRANGYKPFLWDFTFDACEFMRKRNQPVAKIVWNLIKDVSTVNHTCPYVGLQAVSDFHRVEIPVPMPTGEYLLLITWIFDGKPQFSTDVYFTFVEDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSQ9</accession>
    <name>A0A6I8VSQ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein Cpr50Ca</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Cpr50Ca</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234116.1">
        <property type="nucleotide sequence ID" value="XM_033378225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VSQ9"/>
    <dbReference type="FunCoup" id="A0A6I8VSQ9">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805162"/>
    <dbReference type="InParanoid" id="A0A6I8VSQ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF86">
        <property type="entry name" value="CCP84AC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026011676" evidence="4">
        <location>
            <begin position="27"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="128"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="277"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="720"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="727"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033234116.1"/>
        </source>
    </evidence>
    <sequence length="849" mass="95537" checksum="BF50F0707F58DA3B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVJ8</accession>
    <name>A0A6I8VVJ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="35">Protocadherin-like wing polarity protein stan isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">stan</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="35">MV-25-SWS-2005</strain>
            <tissue evidence="35">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033234609.1">
        <property type="nucleotide sequence ID" value="XM_033378718.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VVJ8">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VVJ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001737">
        <property type="term" value="P:establishment of imaginal disc-derived wing hair orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016318">
        <property type="term" value="P:ommatidial rotation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050769">
        <property type="term" value="P:positive regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048056">
        <property type="term" value="P:R3/R4 cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120035">
        <property type="term" value="P:regulation of plasma membrane bounded cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035159">
        <property type="term" value="P:regulation of tube length, open tracheal system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15441">
        <property type="entry name" value="7tmB2_CELSR_Adhesion_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000202">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000173">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000021">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000011">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000059">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000010">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000029">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000044">
        <property type="entry name" value="Cadherin, EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000045">
        <property type="entry name" value="Slit guidance ligand 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Starry night"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000239">
        <property type="entry name" value="Starry night, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000256">
        <property type="entry name" value="Starry night, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000031">
        <property type="entry name" value="Starry night, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
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    <dbReference type="InterPro" id="IPR056286">
        <property type="entry name" value="Cadherin_CELSR1-3_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF51">
        <property type="entry name" value="PROTOCADHERIN-LIKE WING POLARITY PROTEIN STAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23592">
        <property type="entry name" value="Cadherin_CELSR2_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
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    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="16 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 22" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20 23" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="15 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 26" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0807">Transducer</keyword>
    <keyword evidence="9 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 25" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026135294" evidence="26">
        <location>
            <begin position="32"/>
            <end position="3636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="2826"/>
            <end position="2846"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="2897"/>
            <end position="2917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="2929"/>
            <end position="2949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="2969"/>
            <end position="2989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="3010"/>
            <end position="3032"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="3038"/>
            <end position="3061"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="33">
        <location>
            <begin position="365"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="33">
        <location>
            <begin position="470"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="33">
        <location>
            <begin position="587"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="33">
        <location>
            <begin position="695"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="33">
        <location>
            <begin position="800"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="33">
        <location>
            <begin position="903"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="33">
        <location>
            <begin position="1013"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="33">
        <location>
            <begin position="1119"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="28">
        <location>
            <begin position="1487"/>
            <end position="1523"/>
        </location>
    </feature>
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        <location>
            <begin position="1561"/>
            <end position="1759"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="28">
        <location>
            <begin position="1762"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="27">
        <location>
            <begin position="1802"/>
            <end position="1969"/>
        </location>
    </feature>
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        <location>
            <begin position="1971"/>
            <end position="2006"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="29">
        <location>
            <begin position="2101"/>
            <end position="2148"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="31">
        <location>
            <begin position="2133"/>
            <end position="2207"/>
        </location>
    </feature>
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        <location>
            <begin position="2657"/>
            <end position="2812"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="32">
        <location>
            <begin position="2821"/>
            <end position="3062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="2554"/>
            <end position="2588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="2617"/>
            <end position="2638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="2654"/>
            <end position="2693"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="3119"/>
            <end position="3235"/>
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    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="3351"/>
            <end position="3391"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="3466"/>
            <end position="3528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="3557"/>
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        </location>
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        <location>
            <begin position="2571"/>
            <end position="2586"/>
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        <location>
            <begin position="2624"/>
            <end position="2634"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="24">
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            <end position="3137"/>
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            <begin position="3187"/>
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            <begin position="3486"/>
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        <location>
            <begin position="1513"/>
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        <location>
            <begin position="1788"/>
            <end position="1797"/>
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        <location>
            <begin position="1975"/>
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        <location>
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            <begin position="2101"/>
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        <location>
            <begin position="2103"/>
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    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="2122"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023040"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
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        <source>
            <dbReference type="PROSITE" id="PS50027"/>
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        <source>
            <dbReference type="PROSITE" id="PS50221"/>
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        <source>
            <dbReference type="PROSITE" id="PS50227"/>
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    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_033234609.1"/>
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    <sequence length="3636" mass="404458" checksum="C123FEFA39F734D1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VW51</accession>
    <name>A0A6I8VW51_DROPS</name>
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        <submittedName>
            <fullName evidence="8 9">Organic cation transporter protein</fullName>
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        <name evidence="8 9" type="primary">LOC4804165</name>
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    <organism evidence="7 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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            <end position="540"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033234804.1"/>
        </source>
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        <subcellularLocation>
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</entry>
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        <source>
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        <subcellularLocation>
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        <property type="project" value="UniProtKB-ARBA"/>
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            <end position="265"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033235793.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="49839" checksum="B482E05CD6A6A53F" modified="2020-08-12" version="1">MSEGKPWEGIYSVNIPSEPCPCPICFGMFTNAFTFTGKYSWSNTQKNSTRLLIGAYWFFTIIITSCYTGSIIAFVTLPAFPDTVDSVLDLLGLFFRVGTLDNGGWETWFQNSTHIPTSRLYRKMEFVGSLEEGIGNVTQSFFWNYAFLGSKAQLEYLVQSNFSDENISRRSALHLSEECFALFQIGFLFPRESVYKRKIDSMILLAQQSGLISKISNEVSWVMQRSTSGRLLQASSSSSLREIIQEERQLTTADTEGMFLLMALGYFLGGTALVSEIVGGITNKCRQIIKRSRKSASSSWSSQKSSALGLGGERTSAQKIEHDERKAARRDAADDVQKMSFGMRELNLTRTTFRELYGNYSRADQQQSCGHKPSDLVQSHEHTAQNESLDCLDELIAHLEKAEAQSDPSSAQQFFPDEYFGANTEHASVECFGPNTEQSSDTTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0P0</accession>
    <name>A0A6I8W0P0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein OZF-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC26533994</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236319.1">
        <property type="nucleotide sequence ID" value="XM_033380428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0P0"/>
    <dbReference type="InParanoid" id="A0A6I8W0P0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000450">
        <property type="entry name" value="PR domain zinc finger protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000086">
        <property type="entry name" value="transcription factor E4F1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000340">
        <property type="entry name" value="zinc finger protein 473 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000093">
        <property type="entry name" value="zinc finger protein 668 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF23">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="14">
        <location>
            <begin position="33"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="454"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033236319.1"/>
        </source>
    </evidence>
    <sequence length="478" mass="56059" checksum="BDAD226FE02BFFF6" modified="2020-08-12" version="1">MFCGQNKQFVPKTPQSKQFAIITQSFTCRTMEEICRVCMANSGAFTNIFDERPTLDTCIADMIAQCTGYVVRRGDLLPENICPPCLEDAVSAFTLKKTLEHSHKLYFPVMEEYREEDICDNLEDEDWEPSDIESEQSIQFEPHAEVQSDKDLDNLFKCSLCPKSYTRQSNLNRHKKIHNVERPYKCADCPKSFHQKSQLQSHTRTHSGDQPYQCSYCSKFFANKYNLERHTHKHTDDRPFKLSQCSMSFKQIAHRQIQNRTQLGDRLYQCSHLQDHTRFPSSKKHYLCSHCTKSFSSQGYLKIHTRTHTGERPYICSHCSKSFSQLYCFKRHLRWHRNERPFKCSDCSKSFTEKHNLKAHSRTHTVERPLQCCHCPESFQEKESLKLHIQKCAIERPYQCTHCSKSFKQKAHHSAHIRTHTGERPHKCSHCSKSFTQLSHLQIHIRTHTGERPYQCSHCSKTFVQGSTFNRHIRSQHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W188</accession>
    <name>A0A6I8W188_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27 28 29 30" type="primary">Eph</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28 29" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27 28 29">MV-25-SWS-2005</strain>
            <tissue evidence="27 28 29">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033237097.1">
        <property type="nucleotide sequence ID" value="XM_033381206.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237098.1">
        <property type="nucleotide sequence ID" value="XM_033381207.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237099.1">
        <property type="nucleotide sequence ID" value="XM_033381208.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237100.1">
        <property type="nucleotide sequence ID" value="XM_033381209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W188"/>
    <dbReference type="KEGG" id="dpo:6899822"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243947">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10319">
        <property type="entry name" value="EphR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05033">
        <property type="entry name" value="PTKc_EphR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000080">
        <property type="entry name" value="Eph receptor tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000089">
        <property type="entry name" value="Eph receptor tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001638">
        <property type="entry name" value="Eph receptor tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001644">
        <property type="entry name" value="Eph receptor tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000005">
        <property type="entry name" value="Eph receptor tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000019">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000143">
        <property type="entry name" value="Ephrin type-B receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF14">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 18 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 27 28" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="640"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="25">
        <location>
            <begin position="86"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="392"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="534"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="748"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="23">
        <location>
            <begin position="1046"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="875"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="754"/>
            <end position="762"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18 20">
        <location>
            <position position="782"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="135"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_033237097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_033237098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_033237099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_033237100.1"/>
        </source>
    </evidence>
    <sequence length="1103" mass="124375" checksum="828763E2A87A88A8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1Y0</accession>
    <name>A0A6I8W1Y0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237366.1">
        <property type="nucleotide sequence ID" value="XM_033381475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W1Y0"/>
    <dbReference type="KEGG" id="dpo:117184389"/>
    <dbReference type="InParanoid" id="A0A6I8W1Y0"/>
    <dbReference type="OMA" id="MFLIFSY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033237366.1"/>
        </source>
    </evidence>
    <sequence length="65" mass="7441" checksum="DDEC1DB364247A16" modified="2020-08-12" version="1">MQILYNYSPGNNVVDILNPEYNDFDGPPPSKKMIIGSCSMFVIFSYLKLLYILVAIYLALHHAHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5D9</accession>
    <name>A0A6I8W5D9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcriptional repressor scratch 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Scratch homolog 1 zinc finger protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">scrt</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238566.1">
        <property type="nucleotide sequence ID" value="XM_033382675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5D9"/>
    <dbReference type="FunCoup" id="A0A6I8W5D9">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899936"/>
    <dbReference type="InParanoid" id="A0A6I8W5D9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W5D9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055059">
        <property type="term" value="P:asymmetric neuroblast division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060562">
        <property type="term" value="P:epithelial tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000177">
        <property type="term" value="P:regulation of neural precursor cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000043">
        <property type="entry name" value="Scratch family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000169">
        <property type="entry name" value="transcriptional repressor scratch 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001484">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000207">
        <property type="entry name" value="zinc finger protein SNAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF54">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF26.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="505"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="562"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="590"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="618"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="15"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="101"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="205"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="318"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="391"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="101"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="402"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033238566.1"/>
        </source>
    </evidence>
    <sequence length="701" mass="75535" checksum="F4366A423A89CDA2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6S1</accession>
    <name>A0A6I8W6S1_DROPS</name>
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        <recommendedName>
            <fullName evidence="8">TBC1 domain family member 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6900324</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a GTPase-activating protein for RAB35. Together with RAB35 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with RAB1A and RAB10; in a GTP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033238339.1">
        <property type="nucleotide sequence ID" value="XM_033382448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6S1"/>
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        <property type="interactions" value="1341"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W6S1"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W6S1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TBC1 domain family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000019">
        <property type="entry name" value="TBC1 domain family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF27">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="9">
        <location>
            <begin position="34"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238339.1"/>
        </source>
    </evidence>
    <sequence length="404" mass="46075" checksum="6B1DA82F29123EF3" modified="2020-08-12" version="1">MSIFKARVKEFDDALAQDVVDLKELRRLTFNGVPDVQSFRALSWKLLLGYLGPRRSSWTTTLAQKRALYKQFIEELVLPPGHSCNGAGEGDGDGDVAVDSRGVGLQDHPLSEGPESAWNTFFNDNEFLLQIDKDVRRLCPDISFFQQPTEYPCDIVVHSKGEHGRRLHERVVPTVLSSANVERKGLGMTKQQINLITKRSVETYAAMEDGQEAHWEVVQRILFIYAKLNPGQGYVQGMNEIVGPIYYVMASDPDLSYRAHAEADCFFCFTALMSEIRDFFIKTLDDAEGGIKFMMGLLSNMLKTKDIDIYEHLKSQELHPQYYSFRWLTLLLSQEFPLPDVLRIWDSVFSDEQRFNFLIKICCSMILIQREAILENDFASNVKLLQNYPPIDINVVITHAVSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7H8</accession>
    <name>A0A6I8W7H8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Myb-like protein AA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812999</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239242.1">
        <property type="nucleotide sequence ID" value="XM_033383351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W7H8"/>
    <dbReference type="FunCoup" id="A0A6I8W7H8">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812999"/>
    <dbReference type="InParanoid" id="A0A6I8W7H8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF69">
        <property type="entry name" value="SI:CH73-59F11.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="2">
        <location>
            <begin position="12"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239242.1"/>
        </source>
    </evidence>
    <sequence length="420" mass="46533" checksum="A0B4FE140B0609FD" modified="2020-08-12" version="1">MAFSRHNIEKRRLIELVRLNPILWDCRLPHYKRSDKKKAIKWNELGRMFNVTGERVQRTFTSLREIFRRELNHEKMLGTTRFKSKWEYYDAMAFLKEVIRERKSRERVKQGPTDPATRSISTNNNNNNNNSNSNNNNSVAMDEYQYFAPSDPNNPNNQPQPQPQALALPQPKTNVTSVSNPATILPSLSQLPAALLPQAQHLQALQLQPDVTLTSLSAIPKQPPSATTSAVPAAPASPAQVLSSSRSCSSSPSIYIKDEPGSPENADGLGSGTKLATIRPQTKQQLKARLQVPAPKSASIYATSPPRLIINANNELIDADAGDLDEDLDDYDEDDTETGRYSPVVDVDMMGPDGRFSPGGSSYTRVGAVGGGRSQSRHLPSPRELLYMKFGDFLAARLNTLNETVANELMNKILLLIAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAK6</accession>
    <name>A0A6I8WAK6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Protein boule isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">bol</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033240434.1">
        <property type="nucleotide sequence ID" value="XM_033384543.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240435.1">
        <property type="nucleotide sequence ID" value="XM_033384544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WAK6"/>
    <dbReference type="KEGG" id="dpo:4814025"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070935">
        <property type="term" value="P:3'-UTR-mediated mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12412">
        <property type="entry name" value="RRM_DAZL_BOULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000167">
        <property type="entry name" value="protein boule-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034988">
        <property type="entry name" value="DAZ_BOULE_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043628">
        <property type="entry name" value="DAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176">
        <property type="entry name" value="BOULE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11176:SF57">
        <property type="entry name" value="PROTEIN BOULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51890">
        <property type="entry name" value="DAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="DAZ" evidence="11">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="194"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033240434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033240435.1"/>
        </source>
    </evidence>
    <sequence length="273" mass="30362" checksum="101674629DCDCAEC" modified="2020-08-12" version="1">MHKIAAQPPTSAPAGGLDAPLAAPKYGTLIPNRIFVGGISGDTTEADLTRVFSAYGTVKSTKIIVDRAGVSKGYGFVTFETEQEAQRLQSDGECVVLRDRKLNIAPAIKKQVFLFEQPNPLQSIVAANGAVYYTTTPPAPISNIPMDQFAAAVYPPVTDFTAAGVPAIYPPSAMQYQPFYQYYSVPMQNVPTIWPQNYQENHPPPMMAPQQQQQQQQQQQQQQQQQQQIQQQQQQQQQIQQQQQQPMLLPQQPSTSNWMELDDLNVNNCGSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBG3</accession>
    <name>A0A6I8WBG3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239884.1">
        <property type="nucleotide sequence ID" value="XM_033383993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WBG3"/>
    <dbReference type="KEGG" id="dpo:6900802"/>
    <dbReference type="InParanoid" id="A0A6I8WBG3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WBG3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033239884.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="43892" checksum="34521D208E299429" modified="2020-08-12" version="1">MAATVAQRNILDLPYEVLDLIYKKLNSLSDRISLAKADEYLGEAFAFHSRDHFRQVNTYTYFQGDWWPFLLSLCGTHVREFRFSTGHNWSERLVKLVERHCPHLEKVTLCVNNISCKGIRSLVTNASATIKEIDLYVNDSRISEILHEYPDFPQLRKARFCKLSYVDVHSIQKYNTVEDLALQSGSLGHDIPIKLFEITANLKKLRFLKVFHYNLVESGDGDGDGGGDGDALLGMPIPCPNVEYLQMDYCQISMELPLCPKIKSLKISYCISSIEDVLWRFIVKQGGTLEDIEINCRPSLCDSGGFLEILRSCKRLRLIRIQAQKVKFTREVVATMMDILKDNGVTPDRPLRLILYCNAKVFWIAQWLRSNSNRNLISVQEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WD29</accession>
    <name>A0A6I8WD29_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15 16">Protein nervous wreck isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15 16 17" type="primary">nwk</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15 16">MV-25-SWS-2005</strain>
            <tissue evidence="14 15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033241315.1">
        <property type="nucleotide sequence ID" value="XM_033385424.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241316.1">
        <property type="nucleotide sequence ID" value="XM_033385425.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241317.1">
        <property type="nucleotide sequence ID" value="XM_033385426.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241318.1">
        <property type="nucleotide sequence ID" value="XM_033385427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WD29"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WD29">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051495">
        <property type="term" value="P:positive regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902905">
        <property type="term" value="P:positive regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11761">
        <property type="entry name" value="SH3_FCHSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000107">
        <property type="entry name" value="FCH and double SH3 domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000039">
        <property type="entry name" value="FCH and double SH3 domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000155">
        <property type="entry name" value="FCH and double SH3 domains protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035460">
        <property type="entry name" value="FCHSD_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="12">
        <location>
            <begin position="11"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="549"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="666"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="730"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="777"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="873"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1000"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="741"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="828"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="886"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="916"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="948"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1006"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033241315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033241316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033241317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033241318.1"/>
        </source>
    </evidence>
    <sequence length="1102" mass="120230" checksum="F6227FF5AEED207A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEF9</accession>
    <name>A0A6I8WEF9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="6">M1Pi</shortName>
            <shortName evidence="6">MTR-1-P isomerase</shortName>
            <ecNumber evidence="6">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4800729</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="6"/>
    <dbReference type="RefSeq" id="XP_033241805.1">
        <property type="nucleotide sequence ID" value="XM_033385914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEF9"/>
    <dbReference type="FunCoup" id="A0A6I8WEF9">
        <property type="interactions" value="1645"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WEF9"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00874"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WEF9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000010">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000003">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033241805.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="38960" checksum="F476ED57487A2C0B" modified="2020-08-12" version="1">MSLQSIKYTRGSLEILDQLLLPVQSKYLPVRGVEDGWKVINKMQVRGAPAIAIVGCLSLAVEIHPEEFDSKKSLRQELEGKLNYLVSARPTAVNMKMAADELLSLANDLTKDDNVDVAAMKQRFLNATEAMLKKDIADNRAIGAHGAKAILQLVAAAAGAPMSGPVRVLTHCNTGSLATAGYGTALGVVRQLSELGKLEHVYCTETRPYNQGARLTAYELVHEKFPATLVLDSMVAALLRAKNVAAVVVGADRVAANGDTANKIGTYQIAVVAKHHGVPFFVAAPLTSIDLHIPSGDHIIIEERPDREMTHVGEHRIAAPGINCWNPAFDVTPASLITGIITERGVFQPAQLKETITKLLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEJ0</accession>
    <name>A0A6I8WEJ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117185660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241813.1">
        <property type="nucleotide sequence ID" value="XM_033385922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEJ0"/>
    <dbReference type="KEGG" id="dpo:117185660"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079509">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WEJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20913">
        <property type="entry name" value="DCAF15-CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038914">
        <property type="entry name" value="DCAF15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047319">
        <property type="entry name" value="DCAF15_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28541">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28541:SF1">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033241813.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="35881" checksum="F5586D4A3E058D37" modified="2020-08-12" version="1">MLSYKPVGPLGALSPLQVSMAKRFEIGGSMSPNAGLSFLSPRRDEPRIVEMPVQGGVMPEKPVCTKKLRRRYVEEDDATSVITSEEDDCISPGYHTSLPMEVHGSCYSEMQMISHASYQRLRCSSVVITQHSFDLETFTYYVISLLCQKHHKIYNVFYDWAYEVISVCPLSQTVSCLLMAQFSARDQPYAPLTNCLHCTGRIDCMFHNRQYECRILFTWNMESGQWQVLDYGELREMHELFNPLKRLCKSRLTFAQMAKKMAKEMTESLNKLPDYASNLRVLNSDIKKSKSYICDLDNMVEFHLKRTPQGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WET4</accession>
    <name>A0A6I8WET4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Or94a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Odorant receptor which mediates acceptance or avoidance behavior, depending on its substrates. The odorant receptor repertoire encodes a large collection of odor stimuli that vary widely in identity, intensity, and duration. May form a complex with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241792.1">
        <property type="nucleotide sequence ID" value="XM_033385901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WET4"/>
    <dbReference type="FunCoup" id="A0A6I8WET4">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800918"/>
    <dbReference type="InParanoid" id="A0A6I8WET4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF37">
        <property type="entry name" value="ODORANT RECEPTOR 46A, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 13 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033241792.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="44282" checksum="49E3860D62248223" modified="2020-08-12" version="1">MDSHKERIESMHLILQVMRLFGLWPWSLSEGDHWTVWGFVRRNYRFLLHLPITFTFIGLMWLEAFVSSNLEQAGQVLYMSITEMALVVKILSIWHHRSAAWQLMHEFQQAPELRLRSQEEQSFWQREQRYFKWFFYIYILISLGVVYSGCTGVLFLKEYELPFAYYVPFEWRNERGYWFAYGYDMAGMTLTCISNITLDTLGCYFLFHISLLHRLLGMRLRALKSTADETYFGQELRQIFEMHQSVRRLTHTCQAIVSPYILSQIILSALIICFSGYRLQHVGIRANPGQFIAMLQFVSVMILQIYLPCYYGNEITVYAHQLTNEVYHTNWLHCRPPLRKLLNGYMEHLKKPVAIRAGNFFAVGLPIFVKVLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8G9Q9</accession>
    <name>A0ABD8G9Q9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-fold modifier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Ufm1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138444.1">
        <property type="nucleotide sequence ID" value="XM_002138408.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8G9Q9"/>
    <dbReference type="SMR" id="A0ABD8G9Q9"/>
    <dbReference type="GeneID" id="6898397"/>
    <dbReference type="KEGG" id="dpo:6898397"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd01766">
        <property type="entry name" value="Ubl_UFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000044">
        <property type="entry name" value="Ubiquitin-fold modifier 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005375">
        <property type="entry name" value="UFM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15825">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15825:SF0">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03671">
        <property type="entry name" value="Ufm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038027">
        <property type="entry name" value="Ubiquitin-like_Ufm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002138444.1"/>
        </source>
    </evidence>
    <sequence length="84" mass="9110" checksum="7229E71C55776956" modified="2025-06-18" version="1">MSKVTFKITLTSDPKLPFKVLSVPEGTPFTAVLKFASEEFKVPAETSAIITDDGIGISPQQTAGNVFLKHGSELRLIPRDRVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>B5DNS8</accession>
    <accession>A0A6I8V103</accession>
    <name>B5DNS8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vitellogenin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Yp2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134553.2">
        <property type="nucleotide sequence ID" value="XM_002134517.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DNS8"/>
    <dbReference type="FunCoup" id="B5DNS8">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DNS8"/>
    <dbReference type="GeneID" id="6901074"/>
    <dbReference type="KEGG" id="dpo:6901074"/>
    <dbReference type="eggNOG" id="ENOG502SRF1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027171_6_0_1"/>
    <dbReference type="InParanoid" id="B5DNS8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243709">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DNS8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF149">
        <property type="entry name" value="FI01450P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Lipase" evidence="7">
        <location>
            <begin position="124"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002134553.2"/>
        </source>
    </evidence>
    <sequence length="463" mass="51534" checksum="F4B9D13AA1849D86" modified="2016-01-20" version="2">MQYSLAHHPVRVSTDHRSPITMNPLRTLCLMACLVAASMANSNTGSSSRHRSNSLSNIDQPSNWVNPREIEELPSLKQVNLRQLQEMSLEEGATLLDKLYHLSQFNNVFKPEYTPTPSQIKGFIVGERGQKVEFNLNNLVQTVKRQPNFGDDEVTIFIQGLPQSSSQVRKATSKLVRAYQQRYNLQPYPSQQSTSEEQQKSSSEEQQQQGRKQNQEQDDTKTGDLIVINLGDAIEDFEQYATLNVERLGQQIGNCLVELTNAVNVPQEIIHLIGQGPAAHVAGVAGRQFTRQTGHKLRRITGLDPSKIYGKPEEKLSGLARGDADFVDAIHTSAYGMGTPERLANVDFYPNGPSTGVPGADNVVEASMRATRYFAESVRPGNERNFPAVAACSYKEYKQNNGYGKRTYMGIATDYDVQGDYMLQVNSKSPFGRSTPAQTQTGYHAVHEAWRPSAQGQRQGLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D04</accession>
    <accession>A0A6I8UC26</accession>
    <name>Q29D04_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytosol aminopeptidase</fullName>
            <ecNumber evidence="7">3.4.11.1</ecNumber>
            <ecNumber evidence="8">3.4.11.5</ecNumber>
            <ecNumber evidence="21">3.4.13.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Cysteinylglycine-S-conjugate dipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Leucine aminopeptidase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Leucyl aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Proline aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Prolyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">LOC4812730</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="33">MV-25-SWS-2005</strain>
            <tissue evidence="33">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="27">Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides. The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme. For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates. Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-cysteinylglycine + H2O = L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:28783"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:28784"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Release of N-terminal proline from a peptide.</text>
            <dbReference type="EC" id="3.4.11.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>S-benzyl-L-cysteinylglycine + H2O = S-benzyl-L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:62568"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:145802"/>
            <dbReference type="ChEBI" id="CHEBI:145803"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:62569"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>an S-substituted L-cysteinylglycine + H2O = an S-substituted L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:60444"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.13.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:60445"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1" evidence="7"/>
    <dbReference type="EC" id="3.4.11.5" evidence="8"/>
    <dbReference type="EC" id="3.4.13.23" evidence="21"/>
    <dbReference type="RefSeq" id="XP_001353109.3">
        <property type="nucleotide sequence ID" value="XM_001353073.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D04"/>
    <dbReference type="SMR" id="Q29D04"/>
    <dbReference type="FunCoup" id="Q29D04">
        <property type="interactions" value="870"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29D04"/>
    <dbReference type="GeneID" id="4812730"/>
    <dbReference type="KEGG" id="dpo:4812730"/>
    <dbReference type="eggNOG" id="KOG2597">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013734_1_0_1"/>
    <dbReference type="InParanoid" id="Q29D04"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079543">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29D04">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.220.10:FF:000005">
        <property type="entry name" value="cytosol aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000031">
        <property type="entry name" value="cytosol aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF25">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M17 leucyl aminopeptidase N-terminal" evidence="31">
        <location>
            <begin position="68"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="30">
        <location>
            <begin position="240"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00014190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00029605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00030997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00031564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00033172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00045966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF00883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF02789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_001353109.3"/>
        </source>
    </evidence>
    <sequence length="566" mass="62549" checksum="9F019DB8C0053263" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E69</accession>
    <accession>A0A6I8UB49</accession>
    <name>Q29E69_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Regulator of chromosome condensation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Rcc1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352694.1">
        <property type="nucleotide sequence ID" value="XM_001352658.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E69"/>
    <dbReference type="FunCoup" id="Q29E69">
        <property type="interactions" value="2426"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E69"/>
    <dbReference type="GeneID" id="4812548"/>
    <dbReference type="KEGG" id="dpo:4812548"/>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005210_6_1_1"/>
    <dbReference type="InParanoid" id="Q29E69"/>
    <dbReference type="OMA" id="IFVWGTG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070399">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29E69">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00625">
        <property type="entry name" value="RCC1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="44"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="427"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="453"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352694.1"/>
        </source>
    </evidence>
    <sequence length="530" mass="56400" checksum="BCE874E435CDB86A" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EF0</accession>
    <accession>A0A6I8UAZ3</accession>
    <name>Q29EF0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDP-D-glucose phosphorylase 1</fullName>
            <ecNumber evidence="5">2.7.7.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4812246</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specific and highly efficient GDP-D-glucose phosphorylase regulating the levels of GDP-D-glucose in cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GDP-alpha-D-glucose + phosphate = alpha-D-glucose 1-phosphate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30387"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:62230"/>
            <dbReference type="EC" id="2.7.7.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GDPGP1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.78" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001352613.3">
        <property type="nucleotide sequence ID" value="XM_001352577.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EF0"/>
    <dbReference type="SMR" id="Q29EF0"/>
    <dbReference type="FunCoup" id="Q29EF0">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EF0"/>
    <dbReference type="GeneID" id="4812246"/>
    <dbReference type="KEGG" id="dpo:4812246"/>
    <dbReference type="eggNOG" id="KOG2720">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041964_2_0_1"/>
    <dbReference type="InParanoid" id="Q29EF0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077527">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EF0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080048">
        <property type="term" value="F:GDP-D-glucose phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026506">
        <property type="entry name" value="GDPGP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058865">
        <property type="entry name" value="GDPGP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058866">
        <property type="entry name" value="GDPGP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20884">
        <property type="entry name" value="GDP-D-GLUCOSE PHOSPHORYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20884:SF8">
        <property type="entry name" value="GDP-D-GLUCOSE PHOSPHORYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26216">
        <property type="entry name" value="GDPGP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26217">
        <property type="entry name" value="GDPGP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GDPGP1-like N-terminal" evidence="14">
        <location>
            <begin position="91"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="GDPGP1-like C-terminal" evidence="13">
        <location>
            <begin position="281"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF26216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF26217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001352613.3"/>
        </source>
    </evidence>
    <sequence length="412" mass="47618" checksum="130CF1E8E801239C" modified="2016-01-20" version="3">MYARTFRKLTQYYCDAWKLLSARFKQATATELVQRRNSWKSLVTLPVFVVARSVAWKVAMASEEKPKKKRTQSKEKYVTTLEAKAPHYLNSLKVRWEQLHEIPGLFAYQLEKTRQSRVLPGKYQFYTELNPDRTLKRRIPQTIENLNPTFKPKQFNFNKVDALEVMMTIDDVDNTDVQMIINRSPLTRFHTVVCPDVKNNLVQRITAHSLKFCISFMRGLDDSDIRMGYNSPGALASVNHLHFHLLYLPRDLYINNVELQELAGGYVYRLSQSMPTEAICVVFEANDDEAEVQEKVNNLQKLANWMCGQNIPHNLFITQDRRPGKRGSVQVFVFARSHYCVNKDLADFNVGFCELAGYIPVGNSEKLDNLTEPLVIKRIREVTGTAPQALYTRMKQIVDGEDQSIWDQPLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EX9</accession>
    <accession>A0A6I8UAJ7</accession>
    <name>Q29EX9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[acyl-carrier-protein] S-malonyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">[Acyl-carrier-protein] malonyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">beg</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type II malonyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001352434.2">
        <property type="nucleotide sequence ID" value="XM_001352398.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EX9"/>
    <dbReference type="SMR" id="Q29EX9"/>
    <dbReference type="FunCoup" id="Q29EX9">
        <property type="interactions" value="1572"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EX9"/>
    <dbReference type="GeneID" id="4811948"/>
    <dbReference type="KEGG" id="dpo:4811948"/>
    <dbReference type="eggNOG" id="KOG2926">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030558_2_1_1"/>
    <dbReference type="InParanoid" id="Q29EX9"/>
    <dbReference type="OMA" id="AANYNCP"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080619">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EX9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.250:FF:000005">
        <property type="entry name" value="Malonyl-CoA-acyl carrier protein transacylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.250">
        <property type="entry name" value="Malonyl-CoA ACP transacylase, ACP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052760">
        <property type="entry name" value="Mitochondrial_malonyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47170">
        <property type="entry name" value="MALONYL-COA ACP TRANSACYLASE, ACP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47170:SF2">
        <property type="entry name" value="MALONYL-COA:ACP TRANSACYLASE (MAT) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="13">
        <location>
            <begin position="67"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001352434.2"/>
        </source>
    </evidence>
    <sequence length="375" mass="41503" checksum="5A80AFE9972A07D1" modified="2008-10-14" version="2">MLAVRKTRVFGNLSWSRWRSDAAKATTDSASTLGDANKRQQLLNELAEPMDQKRRPAIDPKETSVMLFPGQGTQYVGMAKDLLRFPGARHIFELANQVLNYDLLKICLEGPREKLNRTEHAQLAVMVSSLAALEQLREERPHAIENCVAAAGFSLGEITALVYAGAMPFDKAVKLVQVRATAMQAACDRAAGAMAMILYGPDTNLGEACAKAQQWCLDRGVESPYCGIANYMYPHCKVVAGNVEALEFLEKNAKALKIRRMKRLAVSGAFHTPLMQTAVEPFTKALKSLSLQDPIIRVYSNVDGKPYRNARNILVQLPKQIVRPVKWEQTLHEMYERKQGADFPRTFECGPGKGLLQVLEKVNAKAAQSCLNVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IP3</accession>
    <accession>A0A6I8VH40</accession>
    <name>Q29IP3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-conjugating enzyme E2 H</fullName>
            <ecNumber evidence="8">2.3.2.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">(E3-independent) E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquitin carrier protein H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin-protein ligase H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">UbcE2H</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21 22">MV-25-SWS-2005</strain>
            <tissue evidence="21 22">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with MAEA and WDR26, components of the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.24" evidence="8"/>
    <dbReference type="RefSeq" id="XP_001355551.1">
        <property type="nucleotide sequence ID" value="XM_001355515.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041222.1">
        <property type="nucleotide sequence ID" value="XM_015185736.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IP3"/>
    <dbReference type="STRING" id="46245.Q29IP3"/>
    <dbReference type="GeneID" id="4815711"/>
    <dbReference type="KEGG" id="dpo:4815711"/>
    <dbReference type="eggNOG" id="KOG0416">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_7_5_1"/>
    <dbReference type="OMA" id="KFYVRFK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075347">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000078">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="3 17" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="19">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="152"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="16">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001355551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_015041222.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20875" checksum="C4BAB8AEE3F13982" modified="2006-04-04" version="1">MSSPSAGKRRMDNDVIKLIESKHEVTILGGLNEFHVKFFGPSETPYEGGVWKVRVYLPDNYPFKSPSIGFVNKIYHPNIDESSGTVCLDVINQAWTALYDLSNIFESFLPQLLTYPNPVDPLNRDAAALYLHEPEEYHRKVADYVQRYATEDALRAAQQERESSDSESSMSDYSEDEARDMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MQ7</accession>
    <accession>A0A6I8UJM6</accession>
    <name>Q29MQ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain dehydrogenase/reductase family 16C member 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356572.1">
        <property type="nucleotide sequence ID" value="XM_001356536.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MQ7"/>
    <dbReference type="SMR" id="Q29MQ7"/>
    <dbReference type="FunCoup" id="Q29MQ7">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MQ7"/>
    <dbReference type="GeneID" id="4817365"/>
    <dbReference type="KEGG" id="dpo:4817365"/>
    <dbReference type="eggNOG" id="KOG1201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_5_1"/>
    <dbReference type="InParanoid" id="Q29MQ7"/>
    <dbReference type="OMA" id="DQIQMSL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073891">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MQ7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000498">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF729">
        <property type="entry name" value="MIP05442P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356572.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="36846" checksum="C6188DC6EC6D618D" modified="2006-04-04" version="1">MTSTPGLLHMDRPLRAFYQFFLDLIIFTIKSVFYILESLYYTLLPKRFRKLKNVSGQVVLITGGGGGVGRLIALNFARLEARIVIWDINQEAIKTTVDLLAKSGYNNCKGYVVDISDREQVYQRAGQVIEEVGPVDILINNAGIVCCKPFWELHDRVIQNTYNINIISHYWTVKAFLPHMMRHNRGHIVTVGSVTGMLGTYGCSDYAATKYACIGFHESLLTDLKAHGYDQIQMSLICPYYINTGMFSGVRPRMQPMLEPQYVADRIENAVRCNEVWCVLPNSIRLLTPLKCLLPAKMCWELMSRVIRGPESMMMFQGRGRVAAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NC4</accession>
    <name>CBPA1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Zinc carboxypeptidase A 1</fullName>
            <ecNumber>3.4.17.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA14587</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL33419" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.4.17.-"/>
    <dbReference type="EMBL" id="CH379060">
        <property type="protein sequence ID" value="EAL33419.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NC4"/>
    <dbReference type="SMR" id="Q29NC4"/>
    <dbReference type="FunCoup" id="Q29NC4">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NC4"/>
    <dbReference type="MEROPS" id="M14.A08"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0281819">
        <property type="protein sequence ID" value="FBpp0280257"/>
        <property type="gene ID" value="FBgn0074615"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817137"/>
    <dbReference type="eggNOG" id="KOG2650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019326_2_1_1"/>
    <dbReference type="InParanoid" id="Q29NC4"/>
    <dbReference type="OMA" id="MYKVNSE"/>
    <dbReference type="PhylomeDB" id="Q29NC4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074615">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.340:FF:000002">
        <property type="entry name" value="Carboxypeptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF156">
        <property type="entry name" value="RH39904P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0121">Carboxypeptidase</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000233308" description="Zinc carboxypeptidase A 1" evidence="3">
        <location>
            <begin position="23"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="4">
        <location>
            <begin position="124"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P00730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EAL33419.1"/>
        </source>
    </evidence>
    <sequence length="425" mass="46982" checksum="36E8DFE8A704FAB6" modified="2006-04-04" version="1" precursor="true">MSLTKSLLLALLAVVALAGVSAERARYDNYRLYKANAENAEQLAVLKQLESSSDSILFLDGVHIVGADVKMVVAPHKVPDLLEILGKAEIKYLLQSSDVQKTMDEIDEKVAMKGRAGEAYNWAQYYELDDTYAWLQTLAIENPGVVTLIEGGKTYQGRSILGVKITKSGKEKPGIFLEAGIHAREWIAPAAATYIINQLLTSEVEAVKQLAENYTWYVLPHANPDGYVYTHTTDRMWRKTRTPYQGCFGADPNRNWAFHWNEVGASSNPCSDTYAGPSAFSEIETLSLSNYLASLKGKIQLYISLHAYSQYLLYPYGHTGDLPDNVADFRKVYDVSIAAVNKRYGTTYTGGNIYDAIYPAAGASVDWAYGTQDVRMAFCYELRPSSNSFITGFKLPAAQIVPASEELLDSIVAMAAEVNNLGYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M167</accession>
    <accession>A0A6I8UCG7</accession>
    <name>Q2M167_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353206.2">
        <property type="nucleotide sequence ID" value="XM_001353170.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M167"/>
    <dbReference type="GeneID" id="4813112"/>
    <dbReference type="KEGG" id="dpo:4813112"/>
    <dbReference type="HOGENOM" id="CLU_119221_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M167"/>
    <dbReference type="OMA" id="DVRYKFI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072358">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353206.2"/>
        </source>
    </evidence>
    <sequence length="149" mass="16567" checksum="F85EE15CE3A0A6F7" modified="2008-10-14" version="2">MFKSKALRCEETGLDAGLQECQKLKDDLGDCLNIVERLSRKNSDPVEGRLSVDCLGSVSTLETSSCPDLSSSSLLLQDSEGNQMNTPAEARSGQKLRSSYVDIRTKCIKLKRELEGTVSLAKHISGLAHRQRNRDYSIPEEKPPTTWRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M168</accession>
    <accession>A0A6I8UCA4</accession>
    <name>Q2M168_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioredoxin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353205.2">
        <property type="nucleotide sequence ID" value="XM_001353169.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M168"/>
    <dbReference type="SMR" id="Q2M168"/>
    <dbReference type="FunCoup" id="Q2M168">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M168"/>
    <dbReference type="GeneID" id="4813900"/>
    <dbReference type="KEGG" id="dpo:4813900"/>
    <dbReference type="eggNOG" id="KOG0907">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_14_0_1"/>
    <dbReference type="InParanoid" id="Q2M168"/>
    <dbReference type="OMA" id="TLQKFCG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072357">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353205.2"/>
        </source>
    </evidence>
    <sequence length="141" mass="15448" checksum="8FA71E6DA71EAFC4" modified="2008-10-14" version="2">MANANKKVIVVESKTNFENIIGEAGNKHVLVEFFATWCGPCALIGPRLEQMANEYAGRMIILKIDVDDHEELAVEYEITSMPTFLIIKNKVTLTQFVGSNAEKVESNVVKFVGKPEGVKTASPEAAGTSRAVTETTKMDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M187</accession>
    <accession>A0A6I8UCF0</accession>
    <name>Q2M187_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Prosbeta2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Non-catalytic component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353186.1">
        <property type="nucleotide sequence ID" value="XM_001353150.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M187"/>
    <dbReference type="FunCoup" id="Q2M187">
        <property type="interactions" value="1698"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M187"/>
    <dbReference type="MEROPS" id="T01.A02"/>
    <dbReference type="GeneID" id="4812987"/>
    <dbReference type="KEGG" id="dpo:4812987"/>
    <dbReference type="eggNOG" id="KOG0173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_3_0_1"/>
    <dbReference type="InParanoid" id="Q2M187"/>
    <dbReference type="OMA" id="KQHLFRH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077395">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000005">
        <property type="entry name" value="Proteasome subunit beta type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024689">
        <property type="entry name" value="Proteasome_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12465">
        <property type="entry name" value="Pr_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 11 14" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <feature type="domain" description="Proteasome beta subunit C-terminal" evidence="12">
        <location>
            <begin position="231"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001353186.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="29731" checksum="206D519C26B31827" modified="2006-02-21" version="1">MDLDLARNLPHAGFNFDNCKRNATLLSGGFKPPTTTKTGTTIVGIIYKDGVVLGADTRATEGPIVSDKNCAKIHYLAKNIYCCGAGTAADTERTTDIISSQLELHRLSTERDVRVVAANTMLKQMLFRYQGHISAALVLGGVDKTGPHIYCIHPHGSSDKLPYATMGSGSLAAMSVFESRWKPDMSENEAKLLVRDAIAAGIFNDLGSGSNVDLCVIRKDSVEYLRNYELANKKGERQLDYRFDIGATRVLHSTISDMNITERIEVVPMETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBA8</accession>
    <name>A0A6I8UBA8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glycerol kinase 5</fullName>
            <ecNumber evidence="4">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP:glycerol 3-phosphotransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC4812186</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Skin-specific kinase that plays a key role in glycerol metabolism, catalyzing its phosphorylation to produce sn-glycerol 3-phosphate. Involved in skin-specific regulation of sterol regulatory element-binding protein (SREBP) processing and lipid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001352729.4">
        <property type="nucleotide sequence ID" value="XM_001352693.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBA8"/>
    <dbReference type="FunCoup" id="A0A6I8UBA8">
        <property type="interactions" value="412"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812186"/>
    <dbReference type="InParanoid" id="A0A6I8UBA8"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBA8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07793">
        <property type="entry name" value="ASKHA_NBD_FGGY_GK5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000102">
        <property type="entry name" value="Putative glycerol kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000104">
        <property type="entry name" value="putative glycerol kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037444">
        <property type="entry name" value="GK5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF68">
        <property type="entry name" value="GLYCEROL KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="8 14 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="15">
        <location>
            <begin position="29"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="16">
        <location>
            <begin position="313"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001352729.4"/>
        </source>
    </evidence>
    <sequence length="553" mass="61826" checksum="4A00AAA3B8BB6341" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBF9</accession>
    <name>A0A6I8UBF9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4811978</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352871.2">
        <property type="nucleotide sequence ID" value="XM_001352835.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBF9"/>
    <dbReference type="FunCoup" id="A0A6I8UBF9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811978"/>
    <dbReference type="InParanoid" id="A0A6I8UBF9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBF9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026011299" evidence="2">
        <location>
            <begin position="24"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352871.2"/>
        </source>
    </evidence>
    <sequence length="165" mass="18482" checksum="774D379E1A63A575" modified="2020-08-12" version="1">MFSHTVRVFKMMYIVAGILISNAEICDTVDRIQIAPRVDLKPEFEQRALTFGGNSGSRSGSRSRSHSRSNSGSSSGEESGERKRGRRVSVTGLAGGVVRGVTSQVSKRVGHRFGWLYDVRLILQQWRSLALSFNLWTIPNFIVECVTSYMSKKLLINSDCDMIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBU5</accession>
    <name>A0A6I8UBU5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription termination factor 3, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mTerf3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mTERF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352993.2">
        <property type="nucleotide sequence ID" value="XM_001352957.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBU5"/>
    <dbReference type="FunCoup" id="A0A6I8UBU5">
        <property type="interactions" value="1411"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812563"/>
    <dbReference type="InParanoid" id="A0A6I8UBU5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBU5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061668">
        <property type="term" value="P:mitochondrial ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.70.10:FF:000002">
        <property type="entry name" value="transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF112">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001352993.2"/>
        </source>
    </evidence>
    <sequence length="353" mass="40943" checksum="7C2B47380483806D" modified="2020-08-12" version="1">MFGSAIRNIIKSSQNAAKFEAICAQCRHIRANRSIQSEVDTIKSVDVATKPNDKELEPAPEHSSAVALDFGSREAHVPSFNLAAYVNSSSTLQQLVSLGVDLHSIERRKGLGEFVLRLDFEKNIKPCLSFLADQGIAPDDFGKMVTKNPLLFKEDLDDLQTRVEYLKSKRFSDEARQRIFTQNPFWLMFSTKRVDRRLGYFQKEFRLSGHDLRLMATKEPNLITYNMEHLRKSVFTLREEMGFSARELQSLIVRKPRLMMIRPDDLVERFSYIHKDMGLPHSQIVQCAELLASREFRLRERHEFLKLLGRAQYDPQKDLYISPRTIVLGNNFYFVRNVAKSDLETFDLFLKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UC15</accession>
    <name>A0A6I8UC15_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Cyp40</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353077.2">
        <property type="nucleotide sequence ID" value="XM_001353041.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UC15"/>
    <dbReference type="FunCoup" id="A0A6I8UC15">
        <property type="interactions" value="2233"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812694"/>
    <dbReference type="InParanoid" id="A0A6I8UC15"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000044">
        <property type="entry name" value="Cyclophilin 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF497">
        <property type="entry name" value="CYCLOPHILIN 40, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="321"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="327"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353077.2"/>
        </source>
    </evidence>
    <sequence length="384" mass="43085" checksum="EE306C32E962999A" modified="2020-08-12" version="1">MDGRKLLQPVRATNPIVFMDIDIGLEDAGRMVIELRKDVVPKTAENFRALCTGECGIGQLGQPLHYKGNRFHRIKRVFAAQSGDVVKNDGTSGESIYGPVFEDENFQLTHNEEGVVSMANYGSPNTNNSQFFVTAVGCENLNGINVVVGRVLRGLGIVAEMELNCNNEGDPTTEIVIRNCGELDPGEDWGIECSDETPDKLPPYPMDWTGKLDKCTSDEAVDLLTGVRQSGNHFYKLGRYHEARAKYRKANRYYHYLSNQLGWQPLRNLKKTSSDDASMRKLEAFFTVNSINSAAVDLKLGNYTSAKYDCSEAIRLDPRCSKAFYRRGQANRALRNYEEAINDLKSAHALLPENKQILNELNATKQLLADYNRQQRKALKNLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCP3</accession>
    <name>A0A6I8UCP3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KxDL motif-containing protein CG10681</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4813195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KXD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353400.2">
        <property type="nucleotide sequence ID" value="XM_001353364.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCP3"/>
    <dbReference type="FunCoup" id="A0A6I8UCP3">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813195"/>
    <dbReference type="InParanoid" id="A0A6I8UCP3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039843">
        <property type="entry name" value="KXD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019371">
        <property type="entry name" value="KxDL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13511">
        <property type="entry name" value="KXDL MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13511:SF0">
        <property type="entry name" value="KXDL MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10241">
        <property type="entry name" value="KxDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KxDL" evidence="2">
        <location>
            <begin position="37"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353400.2"/>
        </source>
    </evidence>
    <sequence length="216" mass="24050" checksum="3036E647449E5C30" modified="2020-08-12" version="1">MSHIYIQDPNQSEDATPDLESYTGFGNSAAEAFIQNLAGMVNQGDVETMIRAQKQMLQRFEKTNEMLLNCNALSQSRLKNANDDFKRHVKCLNEMKKDLDHIFRKIRVIKQKLQQQYPAIYADVQPQRSSLAEEAEDDTDSCAKNSVETVAPTVKASTSTSATGPKKSEATIEYVQMEEAVDNGTVEIENELIKRVCSVETANPNDSSDCTSEDTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDD6</accession>
    <name>A0A6I8UDD6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spermine oxidase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353722.3">
        <property type="nucleotide sequence ID" value="XM_001353686.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDD6"/>
    <dbReference type="FunCoup" id="A0A6I8UDD6">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812871"/>
    <dbReference type="InParanoid" id="A0A6I8UDD6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDD6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF398">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353722.3"/>
        </source>
    </evidence>
    <sequence length="471" mass="53071" checksum="3C540B6FE81032A3" modified="2020-08-12" version="1">MRQSRETARILVVGAGASGIASATRLLEKGFNNVQILEAESRIGGRIHTIPFSENVVDLGAQWCHGEKGNAVHELVKDLYLLERTGDLYSTVRCVRSNKEEVPQELAITLRGIASSSVPDGSNPYKGSVGDYLTQRYWKEIDTQLPVDRVLANEMLESFKRSESSFEGSDNLLEVSGRGHLEFAESEGDQLLNWRDQGFERFLRLLMSASDQPDDLGVLKGRVHFEKKVTEINCDCPCNLNVQCSDGETFNADHVICTVSLGVLQEQHETLFVPALPAAKVNAIKSLKLGTVDKFYMEFAAPPFPTDCAGFYCLWIEQDLQELRSSELFWLESISGCHRVTYQPRLLEAWIAGEHARHMETLKEEKVLEGLSWLFRKFLSFDVPQPNRFVRTQWHSNPNFRGSYSFRTTLADELNTGPWDLQTPVMSDNGHPILLFAGEASSKTHYSTVHGAVEAGWREAERLNDFYQDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFS2</accession>
    <name>A0A6I8UFS2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein mei-218</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mei-218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354736.3">
        <property type="nucleotide sequence ID" value="XM_001354700.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFS2"/>
    <dbReference type="KEGG" id="dpo:4814599"/>
    <dbReference type="InParanoid" id="A0A6I8UFS2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFS2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001354736.3"/>
        </source>
    </evidence>
    <sequence length="1002" mass="110929" checksum="510BC7FCB89AEB04" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGF9</accession>
    <name>A0A6I8UGF9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor SPT6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transcription elongation factor spt6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Spt6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Histone H3-H4 chaperone that plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SPT6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355099.4">
        <property type="nucleotide sequence ID" value="XM_001355063.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UGF9">
        <property type="interactions" value="2854"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814900"/>
    <dbReference type="InParanoid" id="A0A6I8UGF9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00164">
        <property type="entry name" value="S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2740:FF:000001">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.650:FF:000002">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.850:FF:000003">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.850:FF:000004">
        <property type="entry name" value="Transcription elongation factor SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3500.10:FF:000006">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.140:FF:000004">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000030">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000089">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2740">
        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.850">
        <property type="entry name" value="Spt6, helix-hairpin-helix domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028083">
        <property type="entry name" value="Spt6_acidic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042066">
        <property type="entry name" value="Spt6_death-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028088">
        <property type="entry name" value="Spt6_HTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055179">
        <property type="entry name" value="Tex-like_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14641">
        <property type="entry name" value="HTH_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14632">
        <property type="entry name" value="SPT6_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22706">
        <property type="entry name" value="Tex_central_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036947">
        <property type="entry name" value="Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158832">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
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        </location>
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            <begin position="1"/>
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            <begin position="1"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
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    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00070625"/>
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        <source>
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    <evidence type="ECO:0000256" key="11">
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355099.4"/>
        </source>
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    <sequence length="1857" mass="210458" checksum="BE24516F2CD95C71" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGI4</accession>
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        <submittedName>
            <fullName evidence="9">DNA repair protein XRCC1</fullName>
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        <name evidence="9" type="primary">XRCC1</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="9">MV-25-SWS-2005</strain>
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        <subcellularLocation>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_001355133.3"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHU4</accession>
    <name>A0A6I8UHU4_DROPS</name>
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            <fullName evidence="19">tRNA-dihydrouridine(47) synthase [NAD(P)(+)]</fullName>
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            <fullName evidence="19">tRNA-dihydrouridine synthase 3</fullName>
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        <name evidence="24" type="primary">Dus3</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <text evidence="13">Catalyzes the synthesis of dihydrouridine, a modified base, in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Mainly modifies the uridine in position 47 (U47) in the D-loop of most cytoplasmic tRNAs. Also able to mediate the formation of dihydrouridine in some mRNAs, thereby regulating their translation.</text>
    </comment>
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        <reaction evidence="14">
            <text>5,6-dihydrouridine(47) in tRNA + NAD(+) = uridine(47) in tRNA + NADH + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:74443"/>
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            <dbReference type="Rhea" id="RHEA:53366"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>5,6-dihydrouridine(47) in tRNA + NADP(+) = uridine(47) in tRNA + NADPH + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
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            <dbReference type="ChEBI" id="CHEBI:57783"/>
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            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:53362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the dus family. Dus3 subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001355711.4"/>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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</entry>
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</entry>
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        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
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    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NMDARECEPTOR"/>
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        <location>
            <begin position="1"/>
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        </location>
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    <feature type="chain" id="PRO_5026361880" evidence="20">
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            <begin position="20"/>
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        </location>
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            <begin position="553"/>
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    </feature>
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        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="817"/>
            <end position="838"/>
        </location>
    </feature>
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        <location>
            <begin position="424"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="22">
        <location>
            <begin position="434"/>
            <end position="497"/>
        </location>
    </feature>
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        <location>
            <begin position="873"/>
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        <location>
            <begin position="875"/>
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            <begin position="887"/>
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        </location>
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    <feature type="binding site" evidence="15">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="679"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="680"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="730"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="16">
        <location>
            <position position="658"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="82"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="742"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_001356082.2"/>
        </source>
    </evidence>
    <sequence length="898" mass="103100" checksum="F56EBA046041EEA4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIY5</accession>
    <name>A0A6I8UIY5_DROPS</name>
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        <recommendedName>
            <fullName evidence="1">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Mitochondrial DNA polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PolG1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
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        <property type="nucleotide sequence ID" value="XM_001356205.4"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005760">
        <property type="term" value="C:gamma DNA polymerase complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DNA polymerase family A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA polymerase subunit gamma-1, mitochondrial"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.420.390:FF:000004">
        <property type="entry name" value="DNA polymerase subunit gamma-1, mitochondrial"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR002297">
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    </dbReference>
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        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041336">
        <property type="entry name" value="DNApol_Exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267:SF0">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18136">
        <property type="entry name" value="DNApol_Exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00867">
        <property type="entry name" value="DNAPOLG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0239">DNA-directed DNA polymerase</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="7">
        <location>
            <begin position="794"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356241.4"/>
        </source>
    </evidence>
    <sequence length="1153" mass="130470" checksum="BD55A3AF3B86CD3F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULD1</accession>
    <name>A0A6I8ULD1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">mRNA cap guanine-N(7) methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">mRNA (guanine-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">mRNA cap methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Rnmt</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:67008"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.1.1.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.56" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001357357.3">
        <property type="nucleotide sequence ID" value="XM_001357321.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULD1"/>
    <dbReference type="FunCoup" id="A0A6I8ULD1">
        <property type="interactions" value="2240"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817840"/>
    <dbReference type="InParanoid" id="A0A6I8ULD1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULD1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004482">
        <property type="term" value="F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000394">
        <property type="entry name" value="mRNA cap guanine-N7 methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004971">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016899">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039753">
        <property type="entry name" value="RG7MT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189:SF2">
        <property type="entry name" value="MRNA CAP GUANINE-N7 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189">
        <property type="entry name" value="MRNA GUANINE-7- METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03291">
        <property type="entry name" value="mRNA_G-N7_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028762">
        <property type="entry name" value="ABD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51562">
        <property type="entry name" value="RNA_CAP0_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10 16" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10 12" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MRNA cap 0 methyltransferase" evidence="14">
        <location>
            <begin position="84"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>mRNA</name>
            <dbReference type="ChEBI" id="CHEBI:33699"/>
        </ligand>
        <ligandPart>
            <name>mRNA cap</name>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="12">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="12">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="12">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="12">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="12">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="12">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR028762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR028762-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR028762-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001357357.3"/>
        </source>
    </evidence>
    <sequence length="408" mass="46387" checksum="2B1DBCC48CD6913D" modified="2020-08-12" version="1">MSIYEQNVADEKFAKAHKNVSLSDDEESESNAESATTAAPINAGQDDAAGGDDEEAQEGAANTHVVASHYNELKEGGRRDRQKSKIFFMRNFNNWIKSMLINEYMALIKESKRVGDALRVLDMCCGKGGDLLKWEKAAITHIICTDIAEVSVEQCQRRYQDILERAEKSKYANKFTAEFFACDSTLVRLRERYKDVTLQLNLVSCQFAFHYCFESLGQADCMMRNAAECLEPGGYFIATMPDAYEIMRRKREAGPDAQSFGNDVYNIKFDCETDPLPLFGAKYQFHLEGVVDCPEFLVHFPTLVKLGRKYGLKLVRKTTFADYYKEALPQGRNLLQRMSGLESIQPQRCANDDQFAHVQEFQASQRGRPLGTLSKSEWEATTLYLVCAFKKYKNTWDANGKPVFEFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM70</accession>
    <name>A0A6I8UM70_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4800431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357709.3">
        <property type="nucleotide sequence ID" value="XM_001357672.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM70"/>
    <dbReference type="KEGG" id="dpo:4800431"/>
    <dbReference type="InParanoid" id="A0A6I8UM70"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026040422" evidence="2">
        <location>
            <begin position="28"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357709.3"/>
        </source>
    </evidence>
    <sequence length="491" mass="55877" checksum="84FC07768F7073EC" modified="2020-08-12" version="1">MSEYVSRDYLWLCTLLLICALIQVALSQPYQVPHRCSNRLESALEHMRRRSIQTKSSSDRTRSRSMWQPPAQSPYQLYHSFYGQHSDPEDDFYNGGGSYNSGRGYHAKLLHRNGRPYPFADSSAALELEELHQKQQQQQQQQHQQEHRQPHKLAISRVEVEDLKVRVPPAKSASRWVEIDKCKFSTENSTLDTRLIFPDLTLSGKVVMQPMGGRCHMILRLRHAGIEFRTVPLGLDPGSGSSPPSYEQSRRLSASSVRTDSHFAEPGFISVFAHGCQGPTGIRLRQNSKRRFGYGPGAAPQVELGEPHVIMGTTRPQPQRWGKYHKPDYDIRLDHTTQRDKSLELGSDSHGQDSAEFVDVNADNFYRRQFNKRRRRRRRFAENGPTENFVDQLSFNEDVLHFEDEQLQQMVEPDARAFADIFSASGGAGGFGGLNSNGDREELVGEAMSHELEKLFSMGVRGLLTTYMQRALQPAIKETLMENMGYTLSYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMK3</accession>
    <name>A0A6I8UMK3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Chymotrypsin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4800707</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357932.2">
        <property type="nucleotide sequence ID" value="XM_001357895.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMK3"/>
    <dbReference type="KEGG" id="dpo:4800707"/>
    <dbReference type="InParanoid" id="A0A6I8UMK3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000047">
        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026235134" evidence="11">
        <location>
            <begin position="24"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="30"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001357932.2"/>
        </source>
    </evidence>
    <sequence length="265" mass="28122" checksum="CE126AB5231D268B" modified="2020-08-12" version="1">MLSHKNLHLAALVFLAIVGVGHAAPPMGRVVNGTDSSIERYPFVISLRGPTGSHSCGGSIVSKQFVMTAAHCTAGRTADQLTVQYGQTQINSTGPNVVGVKKIIQHELYNPSKNYANDISLLMVQEPFVFDDINVAPVKLPVLNFSTPQTEEGGEGVLIGWGLNATGGSIQTTLQEVNLKVYSDAECTSRHNGSTDPRYHICGGVDEGGMGQCSGDSGGPLLYNGQQVGIVSWSVKPCTVAPYPGVYCKVGNYVDWIKTSQIILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UML5</accession>
    <name>A0A6I8UML5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Myogenic-determination protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nau</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357825.2">
        <property type="nucleotide sequence ID" value="XM_001357788.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UML5"/>
    <dbReference type="FunCoup" id="A0A6I8UML5">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800574"/>
    <dbReference type="InParanoid" id="A0A6I8UML5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045663">
        <property type="term" value="P:positive regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19699">
        <property type="entry name" value="bHLH_TS_dMYOD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000005">
        <property type="entry name" value="Myogenic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002546">
        <property type="entry name" value="MyoD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039704">
        <property type="entry name" value="Myogenic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11534">
        <property type="entry name" value="MYOGENIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11534:SF9">
        <property type="entry name" value="MYOGENIC-DETERMINATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01586">
        <property type="entry name" value="Basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00520">
        <property type="entry name" value="BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="158"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="292"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001357825.2"/>
        </source>
    </evidence>
    <sequence length="328" mass="35718" checksum="6D9259F56143CA2F" modified="2020-08-12" version="1">MTKYHSVGSEMAAGHSIKQEYNNSYPQQPQPGYSSYNPSLGHPNASHNPHQTLQNFFSRFNAVGDPNCGATASASASASASASSCSYGQSGHPADVDKQLAMTMTPSPIYTTDYDDENSSLDSEEHVLAPLVCASAQSSRPCLTWACKACKKKSVTVDRRKAATMRERRRLRKVNEAFEILKRRTSSNPNQRLPKVEILRNAIEYIESLEDLLQESSPSRDGDNLAPSLSGKNCQSDYLSSYAGAYLEDKLSFYNKHMEKYGQFTDFDGNANGSSLDCLNLIVQSINKSTTSTPVKTTATPVSTKTPPTTGAPPATSLHASFKRKCST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ12</accession>
    <name>A0A6I8UQ12_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein Abitram</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Actin-binding transcription modulator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4801873</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABITRAM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358898.2">
        <property type="nucleotide sequence ID" value="XM_001358861.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ12"/>
    <dbReference type="FunCoup" id="A0A6I8UQ12">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801873"/>
    <dbReference type="InParanoid" id="A0A6I8UQ12"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051489">
        <property type="term" value="P:regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039169">
        <property type="entry name" value="Abitram"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13651">
        <property type="entry name" value="PROTEIN ABITRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13651:SF0">
        <property type="entry name" value="PROTEIN ABITRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="188"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358898.2"/>
        </source>
    </evidence>
    <sequence length="219" mass="24561" checksum="3EABF20B78BB8D0F" modified="2020-08-12" version="1">MAATADPLEPFYFEHEEQQRICGKPVSPITDGFDANYPSVVDRFFTRFYYIRANAAYQVLYHSNRICLICLAPTHPALAQGIASLSFDVGNVDRSQNVVKGKAKKGGMILQADSTLALLTTETGQIYKIPSCIRGKLVEVNAALVAEPKLLEHLPEGAGYFAILLPKIENCDLIRESLLTQEQYEEHLKTNQEQNTEHLKTSTEQKEENLKTNEEMLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQA8</accession>
    <name>A0A6I8UQA8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Larval cuticle protein A3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Ccp84Ad</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359220.2">
        <property type="nucleotide sequence ID" value="XM_001359183.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQA8"/>
    <dbReference type="GeneID" id="4802265"/>
    <dbReference type="KEGG" id="dpo:4802265"/>
    <dbReference type="InParanoid" id="A0A6I8UQA8"/>
    <dbReference type="OMA" id="TYTAHAS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF94">
        <property type="entry name" value="CCP84AA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026250412" evidence="4">
        <location>
            <begin position="18"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359220.2"/>
        </source>
    </evidence>
    <sequence length="213" mass="22437" checksum="1DEDD790D6117439" modified="2020-08-12" version="1">MAFKFIALFALIAAANAGLLPAAQVYHAAPAVATYAAPAVVKTIAQPVLAKAAEEYDPHPQYKYAYDVQDSLSGDSKSQVEERDGDVVRGEYSLIDSDGYKRTVQYTADPINGFNAVVNRVPLEHVKTVVKTVAAPAVAHYAAPAVVKTVAPAYHTYAAPAVVKTVAPAYHTYAAPAVVKTVAPVAHYAAPTHYAAPAATYTSYAAPAVAYHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQQ3</accession>
    <name>A0A6I8UQQ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carbonic anhydrase</fullName>
            <ecNumber evidence="8">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CAHbeta</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="8"/>
    <dbReference type="RefSeq" id="XP_001359368.1">
        <property type="nucleotide sequence ID" value="XM_001359331.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQQ3"/>
    <dbReference type="FunCoup" id="A0A6I8UQQ3">
        <property type="interactions" value="353"/>
    </dbReference>
    <dbReference type="GeneID" id="4802450"/>
    <dbReference type="KEGG" id="dpo:4802450"/>
    <dbReference type="InParanoid" id="A0A6I8UQQ3"/>
    <dbReference type="OMA" id="MPNVAAW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071355">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1050.10:FF:000007">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF76">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359368.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="29883" checksum="BD062DF32DB76937" modified="2020-08-12" version="1">MERILRGVMRYRNTTREQMVKEFQKVRDNPEPKAVFFTCMDSRMIPTRYTDTHVGDMFVVRNAGNLIPHAQHFQDEYFSCEPAALELGCVVNDIRHIIVCGHSDCKAMNLLYQLRDPEFASKLNRRLSPLRSWLCTHANTSLEKFQEWHDAGMKDPIFFSSETPLRRFVAYIDSEKKFAIEDKLSQINTLQQMSNIASYGFLKARLESHDLHIHALWFDIYTGDIYYFSRGAKQFIAVDEDSVDRLSAEVRRFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQR0</accession>
    <name>A0A6I8UQR0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heterogeneous nuclear ribonucleoprotein M isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rump</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359437.2">
        <property type="nucleotide sequence ID" value="XM_001359400.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQR0"/>
    <dbReference type="FunCoup" id="A0A6I8UQR0">
        <property type="interactions" value="2187"/>
    </dbReference>
    <dbReference type="GeneID" id="4802536"/>
    <dbReference type="KEGG" id="dpo:4802536"/>
    <dbReference type="InParanoid" id="A0A6I8UQR0"/>
    <dbReference type="OMA" id="GPMNADH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081723">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12385">
        <property type="entry name" value="RRM1_hnRNPM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12386">
        <property type="entry name" value="RRM2_hnRNPM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000492">
        <property type="entry name" value="Blast:Myelin expression factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000531">
        <property type="entry name" value="Myelin expression factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF3">
        <property type="entry name" value="FI21236P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="65"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="243"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="583"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="142"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359437.2"/>
        </source>
    </evidence>
    <sequence length="652" mass="67693" checksum="B448A11B52C3AE3F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URW7</accession>
    <name>A0A6I8URW7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="12">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CCT7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359823.2">
        <property type="nucleotide sequence ID" value="XM_001359786.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URW7"/>
    <dbReference type="FunCoup" id="A0A6I8URW7">
        <property type="interactions" value="2531"/>
    </dbReference>
    <dbReference type="GeneID" id="4803021"/>
    <dbReference type="KEGG" id="dpo:4803021"/>
    <dbReference type="InParanoid" id="A0A6I8URW7"/>
    <dbReference type="OMA" id="HRKGNTW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081000">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000049">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
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        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
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        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
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        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
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        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
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        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
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    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
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    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TCP1_1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TCP1_3"/>
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    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
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        </source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001359823.2"/>
        </source>
    </evidence>
    <sequence length="541" mass="59024" checksum="187DCCCA8424BD1C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US28</accession>
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        <recommendedName>
            <fullName evidence="6">carboxylesterase</fullName>
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        </recommendedName>
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        <name evidence="9" type="primary">alpha-Est4</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
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        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
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        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
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        <property type="entry name" value="COesterase"/>
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
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    </dbReference>
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            <begin position="9"/>
            <end position="522"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359774.3"/>
        </source>
    </evidence>
    <sequence length="541" mass="61307" checksum="F9CCBAE13789C085" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USD5</accession>
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        <submittedName>
            <fullName evidence="6">Protein AF-9</fullName>
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        <name evidence="6" type="primary">ear</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359905.4">
        <property type="nucleotide sequence ID" value="XM_001359868.4"/>
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        <property type="interactions" value="443"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:chromatin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AF-9_AHD"/>
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    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
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    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052790">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47827:SF3">
        <property type="entry name" value="AF-9 ANC1 HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47827">
        <property type="entry name" value="AHD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
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        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="152"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="397"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="433"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="464"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="495"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="528"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="574"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="613"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="640"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="653"/>
            <end position="668"/>
        </location>
    </feature>
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        <location>
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            <end position="686"/>
        </location>
    </feature>
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        <location>
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            <end position="696"/>
        </location>
    </feature>
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        <location>
            <begin position="721"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
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            <end position="812"/>
        </location>
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        <location>
            <begin position="813"/>
            <end position="825"/>
        </location>
    </feature>
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        <location>
            <begin position="845"/>
            <end position="873"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359905.4"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USM2</accession>
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        <submittedName>
            <fullName evidence="13">Mitochondrial thiamine pyrophosphate carrier isoform X1</fullName>
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    <gene>
        <name evidence="13" type="primary">Tpc2</name>
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    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360257.3">
        <property type="nucleotide sequence ID" value="XM_001360220.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USM2"/>
    <dbReference type="FunCoup" id="A0A6I8USM2">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803549"/>
    <dbReference type="InParanoid" id="A0A6I8USM2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8USM2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000202">
        <property type="entry name" value="AT11877p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF10">
        <property type="entry name" value="SLC (SOLUTE CARRIER) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="7"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="110"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="212"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001360257.3"/>
        </source>
    </evidence>
    <sequence length="336" mass="37541" checksum="1DAA9333AC608DC7" modified="2020-08-12" version="1">MPDNTPLVQLLQALGGGVAGGLTRTLVQPFDVIKIRFQMQVEPVGKHGYESKYQGLLHAFRSIYKEEGLRGIWRGHNSGQVLSITYAVVQFWSYEQLRVKAHKMPFFDDRPLLLYFVCGGLAGCLGTVAAQPFDVIRTQVVAADPTSKRSRMSSLRGVHFVHSTEGLRGLSRGLVFTLAQIFPLVGANFLIYKYLNALVLFIVKKSNPDHNIPGPCLFVNGALAGVSSKLLVYPADLMKKRMQLHGFHQDRQTFGRNPICPTAKQCFMTTLKGEGISGFYKGVSPTLLKSGLSSAFYFTFYDYINRYVIHPMQDREEQRLKEEKAKSAAPKKAGWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USY1</accession>
    <name>A0A6I8USY1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4803748</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001360413.3">
        <property type="nucleotide sequence ID" value="XM_001360376.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USY1"/>
    <dbReference type="FunCoup" id="A0A6I8USY1">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803748"/>
    <dbReference type="InParanoid" id="A0A6I8USY1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075038">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF116">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001360413.3"/>
        </source>
    </evidence>
    <sequence length="284" mass="33186" checksum="6B5C5CBA8915B3EE" modified="2020-08-12" version="1">MTLGIFDIPQGDPNPILLAGSPWPMIIILAAYLLFVLKLGRIYMRNRKPYDLRRVLMVYNLGQVIYNGLYFGVVFYYLFIEGICNLRCMESFPPGHRYRQLERVVHTAYLVNKVVDLMDTVFFVLRKSYKQVTFLHIYHHVFMAFGGYAVTRIYGTGGHFNSVGLLNTVVHTVMYFYYFLSSQYPGVKANIWWKKYITLTQLVQFVLLFSYSFYVRFLSANCPIPHSLLYLNMFQGIVFMYLFGKFYIQAYVRPPPVQPESQSQSLSQSQSQSQPQSMQKQKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT61</accession>
    <name>A0A6I8UT61_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein Obp50c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Obp50c</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360534.3">
        <property type="nucleotide sequence ID" value="XM_001360497.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT61"/>
    <dbReference type="FunCoup" id="A0A6I8UT61">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="GeneID" id="4803887"/>
    <dbReference type="KEGG" id="dpo:4803887"/>
    <dbReference type="InParanoid" id="A0A6I8UT61"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052295">
        <property type="entry name" value="Odorant-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066:SF15">
        <property type="entry name" value="GH25962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066">
        <property type="entry name" value="ODORANT-BINDING PROTEIN 59A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026191229" evidence="4">
        <location>
            <begin position="23"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360534.3"/>
        </source>
    </evidence>
    <sequence length="196" mass="21587" checksum="6950FD237A3319A3" modified="2020-08-12" version="1">MDKDRALIICSLSAILYSTIVGSAPAPADVDCSRRQDFNAVRGCCTIPTFHFKAFQKQCGRYMPESGPRVSPCLYDCIFNATGVLTGSEVDAENARSMLKRLLGNNQDFVDVYLDGMLQCPGAVDAMLRSKRPRPMPGVEQCSPVPLYYGICTTKHMFNYCPSSSWSDTELCETARLQNLHCPTMRASRGGGPRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT63</accession>
    <name>A0A6I8UT63_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/potassium-transporting ATPase subunit beta-1-interacting protein</fullName>
            <shortName evidence="7">Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">NKAIN</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11 12">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
            <tissue evidence="10 11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NKAIN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360254.3">
        <property type="nucleotide sequence ID" value="XM_001360217.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039157.2">
        <property type="nucleotide sequence ID" value="XM_015183671.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039161.2">
        <property type="nucleotide sequence ID" value="XM_015183675.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT63"/>
    <dbReference type="KEGG" id="dpo:4803545"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UT63">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008516">
        <property type="entry name" value="Na/K-Atpase_Interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084:SF6">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084">
        <property type="entry name" value="T-CELL LYMPHOMA BREAKPOINT-ASSOCIATED TARGET 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05640">
        <property type="entry name" value="NKAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="250"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="450"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="623"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="634"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360254.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015039157.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015039161.2"/>
        </source>
    </evidence>
    <sequence length="656" mass="73389" checksum="83E63CAD5E9904ED" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU66</accession>
    <name>A0A6I8UU66_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MIP18 family protein galla-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">galla-1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360985.3">
        <property type="nucleotide sequence ID" value="XM_001360948.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU66"/>
    <dbReference type="FunCoup" id="A0A6I8UU66">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804417"/>
    <dbReference type="InParanoid" id="A0A6I8UU66"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000004">
        <property type="entry name" value="cytosolic iron-sulfur assembly component 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF2">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="4">
        <location>
            <begin position="83"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="9"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360985.3"/>
        </source>
    </evidence>
    <sequence length="206" mass="22664" checksum="845DA36C048A7F37" modified="2020-08-12" version="1">MLSYIKRKLSESDGGSSVVPVTSSCGGGVSSNSSSSNSSSGRTSADDLVRKTSQMSMDNEAIAYGEDALLQELGYKNATDLQETIYDLLRGIRDPEKPCTLEDLNVIYEDGIFVLPPTRSNVSVVRIEFNPTVPHCSLATLIGLCIRVKVERGLPHNIKLDIYIKKGAHQTEDEINKQINDKERIAAAMENTNLRQLVENCIKDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUB1</accession>
    <name>A0A6I8UUB1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carboxylesterase 5A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4804318</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360896.2">
        <property type="nucleotide sequence ID" value="XM_001360859.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUB1"/>
    <dbReference type="ESTHER" id="drops-q28zz1">
        <property type="family name" value="OtherNon-catalytic_C"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804318"/>
    <dbReference type="InParanoid" id="A0A6I8UUB1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="3">
        <location>
            <begin position="32"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360896.2"/>
        </source>
    </evidence>
    <sequence length="604" mass="68560" checksum="D8E2530C55FB13F4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUF6</accession>
    <name>A0A6I8UUF6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Gr59f</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361097.5">
        <property type="nucleotide sequence ID" value="XM_001361060.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUF6"/>
    <dbReference type="FunCoup" id="A0A6I8UUF6">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804554"/>
    <dbReference type="InParanoid" id="A0A6I8UUF6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF123">
        <property type="entry name" value="GUSTATORY RECEPTOR FOR SUGAR TASTE 43A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361097.5"/>
        </source>
    </evidence>
    <sequence length="408" mass="47205" checksum="12E8B646BBF235C6" modified="2020-08-12" version="1">MEAFLMSLAVDMGKPKKVPPKHPMTAERLLKGHPSFHQQTRRLYKALHWLLLISVLANTAPIAVLPGRQGIVYRHLHLCWMAVSYGWFCLASYWEFVLITLNKVSIDCYLNAMESAIYVVHSASILILTFQWRHRAPAVIDRIVKSDLERGYSINCRQSKRFLRVQLSLVLVLACSAFAIDICSQRFVVYKAILSIHSFVMPNIISSLSFIQYYVLLQGIAWRQAAVTESLQSELQHLPCPRRWEVQRLRLQHVELTRFTKLVNTAYQYSIVLLIVGCFFNFNLNLFLVYKGIDVPELADWVRWIYMVLWLAMHMGKVYGILYFNHKVQDEQRKCLALLNGVQCVGPDLLDTLNHYVLQLQTNVRQHVVCGVIVLDLKYLSALLVASANFFIFLLQYDVTYEALYKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUM9</accession>
    <name>A0A6I8UUM9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cuticle protein 16.8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Cpr57A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361173.2">
        <property type="nucleotide sequence ID" value="XM_001361136.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUM9"/>
    <dbReference type="FunCoup" id="A0A6I8UUM9">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="GeneID" id="4804644"/>
    <dbReference type="KEGG" id="dpo:4804644"/>
    <dbReference type="InParanoid" id="A0A6I8UUM9"/>
    <dbReference type="OMA" id="VIHATQK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074805">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF173">
        <property type="entry name" value="CUTICULAR PROTEIN 47EF, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026247040" evidence="4">
        <location>
            <begin position="23"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001361173.2"/>
        </source>
    </evidence>
    <sequence length="191" mass="20660" checksum="E1106AA9602B5516" modified="2020-08-12" version="1">MAMKQLMIILVVCALSAPRVASDVSHLVNHETHSPANPYVFSYQAGRAPGHVDRQHTEVSDGSGVIRGAFSYVDPKNQVRTVQYVADENGFHPQLSHKLEDSAAVKAAKRRHFDAYNRIAQEHASHTPGQASQAAAPHASAAVAHATQKHLSDFERIAAEHAAIGAQQEAQRLALAAQSEHGEVEDGQYHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUR0</accession>
    <name>A0A6I8UUR0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Facilitated trehalose transporter Tret1-2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4804683</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Trehalose transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361204.1">
        <property type="nucleotide sequence ID" value="XM_001361167.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUR0"/>
    <dbReference type="FunCoup" id="A0A6I8UUR0">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="GeneID" id="4804683"/>
    <dbReference type="KEGG" id="dpo:4804683"/>
    <dbReference type="InParanoid" id="A0A6I8UUR0"/>
    <dbReference type="OMA" id="APMYCTE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079624">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000055">
        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF1">
        <property type="entry name" value="GH07001P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="12"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361204.1"/>
        </source>
    </evidence>
    <sequence length="464" mass="50436" checksum="7255019B8827449B" modified="2020-08-12" version="1">MSKGSVLPQYIAGLSASFGAMCMGASIGWSSPVEKMITEETDYGFEISSGQFGWISALLTLGATIICIPVGFMIDWIGRRPTMLALIPPYMVGWVLMLFAKNVTMLYFGRFILGMCGGAFCVTAPMYCTEISTTALRGTIGSFFQLLIVSGVFYGYLVGAFVPLTTINILCSILPLIFAAVHIFMPESPVYLAMKGRNEDTAKALQWLRGKDADISEELKEILDEAQKQNDQPKVNVLAALRRPVTRKGLGISVLLQIFQQWTGINAILFYSTSIFEDVGSGLSGSNSTILIGVTQTTTTLVAVAIIDKAGRRILLLISGVFMAITTCLMGVYFQMSESDPDSVVGLGWLPIVSICIFIVFFSIGFGPVPWLVMAELFSEDIKSFGGSIAGTSNWLSAFMVTLLFPILKDSIGPGPTFWIFTAIAVLAFFYALFFVPETKGKTILEIQDMLAGGKVQKPEKNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWV1</accession>
    <name>A0A6I8UWV1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Geminin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">geminin</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the geminin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361854.2">
        <property type="nucleotide sequence ID" value="XM_001361817.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWV1"/>
    <dbReference type="FunCoup" id="A0A6I8UWV1">
        <property type="interactions" value="975"/>
    </dbReference>
    <dbReference type="GeneID" id="4805457"/>
    <dbReference type="KEGG" id="dpo:4805457"/>
    <dbReference type="InParanoid" id="A0A6I8UWV1"/>
    <dbReference type="OMA" id="CEPGENY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076527">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008156">
        <property type="term" value="P:negative regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1180:FF:000004">
        <property type="entry name" value="geminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1180">
        <property type="entry name" value="Geminin coiled-coil domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022786">
        <property type="entry name" value="Geminin/Multicilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13372">
        <property type="entry name" value="GEMININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13372:SF5">
        <property type="entry name" value="GEMININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07412">
        <property type="entry name" value="Geminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111469">
        <property type="entry name" value="Geminin coiled-coil domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="130"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361854.2"/>
        </source>
    </evidence>
    <sequence length="189" mass="21201" checksum="A0D8F64ABFFD60D1" modified="2020-08-12" version="1">MSTGAARVYIQVESEAEQQEHLKQQRKTLKPLHGNVNDKENLSGRASIVDQLSRLKAGVPITPSTKYGKRKCVDTAAAATSTKDADTQTEAQEEPQNDADKPITADDLTSECEPGENYYKLLAEQRRVALEDSLTENRHLHERIEGLEEEMDTMRQELDEAKNLVEVLKEICDEDTSEVEEDETEAAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX71</accession>
    <name>A0A6I8UX71_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">S-phase kinase-associated protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6903597</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132464.2">
        <property type="nucleotide sequence ID" value="XM_002132428.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX71"/>
    <dbReference type="KEGG" id="dpo:6903597"/>
    <dbReference type="InParanoid" id="A0A6I8UX71"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UX71">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000124">
        <property type="entry name" value="Protein CBG09126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="5">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="4">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002132464.2"/>
        </source>
    </evidence>
    <sequence length="142" mass="15786" checksum="025778EE1732B25E" modified="2020-08-12" version="1">MQGIALESSDGEVFVVDVKVVNFSKVLRTMLQSSWLVDDGKPVVLSNITGAILRMVLDWIQYHQDDPQGTEAAEKSSELQEWDANFVNVDQDTLFKLIMAAYFLKIKGLVDVTCKAVANSIKGKTTAELREMFNLNEKNASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXL4</accession>
    <name>A0A6I8UXL4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6903018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132740.3">
        <property type="nucleotide sequence ID" value="XM_002132704.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXL4"/>
    <dbReference type="KEGG" id="dpo:6903018"/>
    <dbReference type="InParanoid" id="A0A6I8UXL4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXL4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002132740.3"/>
        </source>
    </evidence>
    <sequence length="211" mass="24324" checksum="266C2EB16F927141" modified="2020-08-12" version="1">MSKFNQNLPIEINEKLPENIDKNLPKTIEETLPETLNEPKSIFGLTQIESLHEITTKSTTDKELKNLDPQIEKLVIQSLEELQSITQSDATNQDELRMSFIIPKVMSQLQSTNILAERLQAMRTELESCKLHMHDMSKDIGDVFMDLHTIFKTLESKDKKNILSKKDRDRLAKCAHKAETLQELSKHFLINDPRELSAMFKAPKVEPKIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXU3</accession>
    <name>A0A6I8UXU3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Apolipoprotein D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NLaz</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002132893.3">
        <property type="nucleotide sequence ID" value="XM_002132857.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXU3"/>
    <dbReference type="FunCoup" id="A0A6I8UXU3">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903194"/>
    <dbReference type="InParanoid" id="A0A6I8UXU3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXU3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031409">
        <property type="term" value="F:pigment binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19437">
        <property type="entry name" value="lipocalin_apoD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000003">
        <property type="entry name" value="Apolipoprotein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003057">
        <property type="entry name" value="Invtbrt_color"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612:SF34">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08212">
        <property type="entry name" value="Lipocalin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01273">
        <property type="entry name" value="INVTBRTCOLOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026223085" description="Apolipoprotein D" evidence="9">
        <location>
            <begin position="28"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="10">
        <location>
            <begin position="44"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002132893.3"/>
        </source>
    </evidence>
    <sequence length="228" mass="25068" checksum="E4A6AB7912857FE1" modified="2020-08-12" version="1">MIHHRSGWNFLLLVGLLAGNGVWLVHSQVPFPGQCPEVKIMDTFDVDSYLGIWYEHSKYPFAFEIGKKCIYADYEIIDNATLSVVNGGINRITGNPSNISGTAKVIGPARLAVAFFPGQQTSKPNYLVLGTDYESYSVVYSCTSVTSLANIKLVWILTREREPSAETIASAKKILEENKINQSFLIDTIQSKCPKLAANSTDFGVEDLTEDPVDEFVGTVLPNAIDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ66</accession>
    <name>A0A6I8UZ66_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tyrosine-protein phosphatase corkscrew</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">csw</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002133867.3">
        <property type="nucleotide sequence ID" value="XM_002133831.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ66"/>
    <dbReference type="FunCoup" id="A0A6I8UZ66">
        <property type="interactions" value="1582"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901166"/>
    <dbReference type="InParanoid" id="A0A6I8UZ66"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14544">
        <property type="entry name" value="PTPc-N11_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09931">
        <property type="entry name" value="SH2_C-SH2_SHP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000121">
        <property type="entry name" value="Corkscrew, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000012">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000119">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052123">
        <property type="entry name" value="Non-rcpt_Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46257">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE CORKSCREW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46257:SF3">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE CORKSCREW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="239"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="355"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="678"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="73"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="847"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="932"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="879"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="890"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="976"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="992"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002133867.3"/>
        </source>
    </evidence>
    <sequence length="1002" mass="109165" checksum="0796304D73AFCBDE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZA6</accession>
    <name>A0A6I8UZA6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Achaete-scute complex protein T4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sc</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134089.3">
        <property type="nucleotide sequence ID" value="XM_002134053.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZA6"/>
    <dbReference type="FunCoup" id="A0A6I8UZA6">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901403"/>
    <dbReference type="InParanoid" id="A0A6I8UZA6"/>
    <dbReference type="OMA" id="QRQQFPH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061382">
        <property type="term" value="P:Malpighian tubule tip cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19744">
        <property type="entry name" value="bHLH_TS_dAS-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000060">
        <property type="entry name" value="Scute protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF153">
        <property type="entry name" value="ACHAETE-SCUTE FAMILY BHLH TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="107"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="71"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002134089.3"/>
        </source>
    </evidence>
    <sequence length="371" mass="39590" checksum="0B1A191D8EE130CD" modified="2020-08-12" version="1">MSSVVYSSSKMQSQIPVPVPVPDMFPTTISGAAKVFRYQHIMPAPNPGQEIISSGGSSMPIKTRKYTPRGMALERSSASSSSSSSSSSSSSSSVLGAGAPYNVDQSQSVQRRNARERNRVKQVNNSFARLRQHIPQSIIADLTKGGGRGPHKKISKVDTLRIAVEYIRRLQDLVDDLNGGAPASSSAASAVVSQLQLCLDEETCSNSSSSSGSSSHGSHHLYYSSSLAAAAAAAAATTTTTPLQQQQQLQRQQFPHQQPLAPISLNSNLVGVGVGAAAGGATISIPGQTTACHSPTSSFNSSMSFDSGTYEAAPQQLSAPHMELDMELEPEAHDAQLQLKFEPYDHFQLDEEDCTPDDEEILDYISMWQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZK2</accession>
    <name>A0A6I8UZK2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribonuclease P protein subunit p21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Rpp21</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133638.2">
        <property type="nucleotide sequence ID" value="XM_002133602.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZK2"/>
    <dbReference type="FunCoup" id="A0A6I8UZK2">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902107"/>
    <dbReference type="InParanoid" id="A0A6I8UZK2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244106">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZK2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002133638.2"/>
        </source>
    </evidence>
    <sequence length="185" mass="20899" checksum="6FDE14656C5AD5B6" modified="2020-08-12" version="1">MAKNNKKKVFALRLVCSRMNFLYQAAHLMAEGSHNTLGAYYGKLCRNVGTKAIMHMAPALKRTLCKRCSLPLIPGVNTSLTVPQKKDQTAPATDPKACKKRHRRQRRKKGEVGKAKVQPKASTVDEHTPLQLECPLCQGRRRFVVDSQRECWIEQPQALVEVLDIEADEERQQTQAQPQPQSQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0E5</accession>
    <name>A0A6I8V0E5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6900406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134798.2">
        <property type="nucleotide sequence ID" value="XM_002134762.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0E5"/>
    <dbReference type="KEGG" id="dpo:6900406"/>
    <dbReference type="InParanoid" id="A0A6I8V0E5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0E5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026112494" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="186"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002134798.2"/>
        </source>
    </evidence>
    <sequence length="258" mass="26964" checksum="351275B23FB944A7" modified="2020-08-12" version="1">MRNTLAFSAIAALLLVSVAAQECNECQSSNDALCLSTTEYKNCMNNVAIGDTETCPTGTVCSNTAEVCVKSESVDGVNILDVCGGSSGGSSEGNGENCGVCIGSGKFACVSKTQYARCVDQKVSTTPYACGTDETCITEALGTYGTLCVPNCALTFIGLSASTSTCSNTDYTPPTVPSVPTQTELEAACALSVKTNTFFDIDNTADTTCSSYIYCEKLTESTWLAILYSCKSPTPYYSVDANKCTATKPTRCTTQAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1D5</accession>
    <name>A0A6I8V1D5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135563.3">
        <property type="nucleotide sequence ID" value="XM_002135527.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1D5"/>
    <dbReference type="KEGG" id="dpo:6900234"/>
    <dbReference type="InParanoid" id="A0A6I8V1D5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V1D5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002135563.3"/>
        </source>
    </evidence>
    <sequence length="198" mass="23200" checksum="C9F42E9DAC55E48C" modified="2020-08-12" version="1">MPKASSVYGQLERKLICMRLRTSQWEFQCQCLCLCLRMGLLLLLLSPIGRACASGAWKCLYKEFPVDTEKIKGGWWIYGTNPGGETRCYLDGLNLYWTRITMTDYDNYAVELHCSLPWFKHLMAIYTRTQEPTQETLNAVAGYLRTVRLSLHNFTLVAKPENCVNQSHGPWQRWYLARYLHLEYHPHYLKPLIDDENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1P7</accession>
    <name>A0A6I8V1P7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphoribosyl pyrophosphate synthase-associated protein 2 isoform X3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">LOC6896706</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <strain evidence="5 6">MV2-25</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002136920.1">
        <property type="nucleotide sequence ID" value="XM_002136884.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236082.1">
        <property type="nucleotide sequence ID" value="XM_033380191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1P7"/>
    <dbReference type="GeneID" id="6896706"/>
    <dbReference type="KEGG" id="dpo:6896706"/>
    <dbReference type="OMA" id="HYAYARS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248301">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V1P7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000031">
        <property type="entry name" value="Probable PRS4-ribose-phosphate pyrophosphokinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF53">
        <property type="entry name" value="GH23275P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="4">
        <location>
            <begin position="29"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002136920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033236082.1"/>
        </source>
    </evidence>
    <sequence length="407" mass="44958" checksum="81FE0A109669BA5E" modified="2020-08-12" version="1">MLLQRNKLGSVRPPKRCRSDMDNTSTSDIVIINGNSHPDLANMVADRMGLKNGGCSVFHKSNRETIVEISDSVRGKDIYIIQTGTKDANNNIMELLIMAYACKTSSARSIVGVIPYLPYSKQCKMRKRGCIVSKLLAKMMCTSGLTHIITMDLHQKEIQGFFDIPVDNLRASPFLLQYIQESIPEYRNSVIVARNPGVAKKANSYAERLRLGLAVIHGEQKEAESDEVDGRYSPPPTSAYSNLLGNSVEMCFPAAPRNSTPQHAPISSSSRQRTTSVSVGVPEHPVKVKPPLTIVGDVNGRIAIMVDDLIDDVQSFVAAAEMLKENGACKIYVLATHGLLSSDAPRLLDESCIDEIVVTNTIPHEIQKLQCHKIKTIDISILIAEAIRRIHNKESMSYLFRNVTLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1Q8</accession>
    <name>A0A6I8V1Q8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protein O-glucosyltransferase 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC6899754</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Protein O-glucosyltransferase. Catalyzes the reaction that attaches glucose through an O-glycosidic linkage to a conserved serine residue found in the consensus sequence C-X-S-X-[PA]-C in epidermal growth factor-like repeats. Regulates Notch signaling by glucosylating Notch in the ER, glucosylation is required for the correct folding and cleavage of Notch.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[EGF-like domain protein] + UDP-alpha-D-glucose = 3-O-(beta-D-glucosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58116"/>
            <dbReference type="Rhea" id="RHEA-COMP:14610"/>
            <dbReference type="Rhea" id="RHEA-COMP:16010"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140576"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-(beta-D-xylosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62016"/>
            <dbReference type="Rhea" id="RHEA-COMP:16010"/>
            <dbReference type="Rhea" id="RHEA-COMP:16011"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132085"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KDELC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135782.3">
        <property type="nucleotide sequence ID" value="XM_002135746.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1Q8"/>
    <dbReference type="KEGG" id="dpo:6899754"/>
    <dbReference type="InParanoid" id="A0A6I8V1Q8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V1Q8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046527">
        <property type="term" value="F:glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF122">
        <property type="entry name" value="GLYCOSYL TRANSFERASE CAP10 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026134945" evidence="12">
        <location>
            <begin position="23"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="11">
        <location>
            <begin position="24"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="13">
        <location>
            <begin position="230"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002135782.3"/>
        </source>
    </evidence>
    <sequence length="503" mass="59225" checksum="DE3B589AC6D3FCDE" modified="2020-08-12" version="1">MNYQMFSLQYCVISTFLLLSNCQNQILDVAKTQVWGPGLKPDDIVLPARYFFIHAIDNNGKKITSMTHINFQVSIDGYSKDGKCRSNINYIDREDGSVIVRYTIADFCEQVEISVKYNSSHVAQSPYYIQNKVYSERCYCPQNLDIWLLNNRCSDFLKEKQISWDLHLFKRVNFSAIRENILKRYNAPESISLCHYVVKQQQIWRKCFGKYTGFKMFMDATLSALARVVWLPEMEFYLNLGDWPLSRKGGQQRTSGPYPIFSWCGSDDSYDITLPTYDITESTLENMGRVMLDMLSVQQTDIPWDNKEEIGFFRGRDSRRERLKLIDLARKFPDLINASITNFFFFRNEEQKYGPRVPHISFMEFFRYKYQLNIDGAVAAYRLPYLLAGGSLVFKQESPYYEHFYSKLLPYKHYVPIKRDLSDIIEKIRWAKDNDIRAKEIALTARKFVEENLLPQHIYCYHMTLFKEWSNRLISPVKVLPDMEKLSLSYSCSCEKGQNRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2E6</accession>
    <name>A0A6I8V2E6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">LOW QUALITY PROTEIN: synapsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Syn</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synapsin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137199.4">
        <property type="nucleotide sequence ID" value="XM_002137163.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V2E6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897014"/>
    <dbReference type="InParanoid" id="A0A6I8V2E6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007269">
        <property type="term" value="P:neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000029">
        <property type="entry name" value="synapsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000008">
        <property type="entry name" value="Synapsin III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000059">
        <property type="entry name" value="Synapsin-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001359">
        <property type="entry name" value="Synapsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020898">
        <property type="entry name" value="Synapsin_ATP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020897">
        <property type="entry name" value="Synapsin_pre-ATP-grasp_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10841">
        <property type="entry name" value="SYNAPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10841:SF17">
        <property type="entry name" value="SYNAPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02078">
        <property type="entry name" value="Synapsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02750">
        <property type="entry name" value="Synapsin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01368">
        <property type="entry name" value="SYNAPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Synapsin pre-ATP-grasp" evidence="6">
        <location>
            <begin position="85"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Synapsin ATP-binding" evidence="7">
        <location>
            <begin position="188"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="395"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="542"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="841"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="963"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="405"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="456"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="490"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="573"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="613"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="651"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="665"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="704"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="848"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="964"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002137199.4"/>
        </source>
    </evidence>
    <sequence length="993" mass="103549" checksum="616A39FB19964D61" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2Y1</accession>
    <name>A0A6I8V2Y1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Guanine nucleotide-binding protein-like 3 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ns1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play a role in regulating cellular proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137602.2">
        <property type="nucleotide sequence ID" value="XM_002137566.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2Y1"/>
    <dbReference type="FunCoup" id="A0A6I8V2Y1">
        <property type="interactions" value="1849"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897454"/>
    <dbReference type="InParanoid" id="A0A6I8V2Y1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000002">
        <property type="entry name" value="Guanine nucleotide-binding protein-like 3 (nucleolar)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000571">
        <property type="entry name" value="Guanine nucleotide-binding protein-like NSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="10">
        <location>
            <begin position="141"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="60"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002137602.2"/>
        </source>
    </evidence>
    <sequence length="583" mass="65996" checksum="AF804D47304BD27E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2Z4</accession>
    <name>A0A6I8V2Z4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein light intermediate chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6897544</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the dynein light intermediate chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137690.3">
        <property type="nucleotide sequence ID" value="XM_002137654.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2Z4"/>
    <dbReference type="KEGG" id="dpo:6897544"/>
    <dbReference type="InParanoid" id="A0A6I8V2Z4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008467">
        <property type="entry name" value="Dynein1_light_intermed_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022780">
        <property type="entry name" value="Dynein_light_int_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688:SF0">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05783">
        <property type="entry name" value="DLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="425"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="367"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002137690.3"/>
        </source>
    </evidence>
    <sequence length="451" mass="50417" checksum="122C0B53063196B2" modified="2020-08-12" version="1">MAAEKPEEWPAKMTKAQDKENLWLDILHEQKGAARRLVANKSVIVFGDNGTGKTSLLAKLNGRKSPEYGVSLEYDYIDVRDDINGDKAQLVVWTINGDIGHTNLLPVVFNEDNFGDVLVIITVSITEPWAWPKQLKHWVNVLERHIESLPLAAEKKVQARERLATIWQSYGYTGSCLKQAIAADDLSPLTEDALTTNLGVDLVVVVTKTDCMGGTFLMEHDYCDEHIDFMQHWIRTFCLRHGAALFYTSVKKATNCDHLRKYLLHRIYGLPFQTPPMVVNEDAVLIPAGWDTLRKIDILFESIHGIKANSDYTDIIKAPSTGNVIFNRNSEFVETVDEQAFLAHQMEILKLLDQSKAKEGGDGIHCTRQSSTGQSSSMKTEHYESPGRIQVLSKFFAELREKKSESPSGDGPVSVSSRNAIEKLGISLQPGAELGPLSRIAEKKEDQVKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3D3</accession>
    <name>A0A6I8V3D3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-conjugating enzyme E2-18 kDa</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ubc84D</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="RefSeq" id="XP_002137965.2">
        <property type="nucleotide sequence ID" value="XM_002137929.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3D3"/>
    <dbReference type="KEGG" id="dpo:6897863"/>
    <dbReference type="InParanoid" id="A0A6I8V3D3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23801">
        <property type="entry name" value="UBCc_UBE2L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000011">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002137965.2"/>
        </source>
    </evidence>
    <sequence length="154" mass="17766" checksum="154E7D38F10AF789" modified="2020-08-12" version="1">MSATRRLTRELADLLGSVEKKTWCNIQASDESIMVWKILLVPERPPYNKGAFRIEVTFPDVYPFQPPKVMFKTKIYHPNIDETGLVCLPIISSDNWKPTTSTEKVLEALVGILHEPEPEHSLRADVAEEFVTNRKKFMKNAEEFTKKNAEKRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V586</accession>
    <name>A0A6I8V586_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sugar phosphate exchanger 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Solute carrier family 37 member 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MFS16</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glucose 6-phosphate(in) + phosphate(out) = D-glucose 6-phosphate(out) + phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:71535"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138302.3">
        <property type="nucleotide sequence ID" value="XM_002138266.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V586"/>
    <dbReference type="FunCoup" id="A0A6I8V586">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898236"/>
    <dbReference type="InParanoid" id="A0A6I8V586"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061513">
        <property type="term" value="F:glucose 6-phosphate:phosphate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17344">
        <property type="entry name" value="MFS_SLC37A1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000028">
        <property type="entry name" value="Sugar phosphate exchanger 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044740">
        <property type="entry name" value="SLC37A1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF12">
        <property type="entry name" value="SUGAR PHOSPHATE EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="13">
        <location>
            <begin position="53"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002138302.3"/>
        </source>
    </evidence>
    <sequence length="533" mass="58381" checksum="96A39C6708DBD6CD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V589</accession>
    <name>A0A6I8V589_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein stops isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">stops</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736200.3">
        <property type="nucleotide sequence ID" value="XM_003736152.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V589"/>
    <dbReference type="FunCoup" id="A0A6I8V589">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800341"/>
    <dbReference type="InParanoid" id="A0A6I8V589"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V589">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03587">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039147">
        <property type="entry name" value="ASB17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20966">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20966:SF2">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SOCS box" evidence="4">
        <location>
            <begin position="363"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="407"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="488"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_003736200.3"/>
        </source>
    </evidence>
    <sequence length="520" mass="59245" checksum="3EC64843AFE0C914" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5W8</accession>
    <name>A0A6I8V5W8_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Calmodulin-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6898742</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138729.1">
        <property type="nucleotide sequence ID" value="XM_002138693.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5W8"/>
    <dbReference type="FunCoup" id="A0A6I8V5W8">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="GeneID" id="6898742"/>
    <dbReference type="KEGG" id="dpo:6898742"/>
    <dbReference type="InParanoid" id="A0A6I8V5W8"/>
    <dbReference type="OMA" id="NKGCITC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245638">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000527">
        <property type="entry name" value="Calmodulin-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="9"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="82"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="118"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002138729.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="17030" checksum="D04C456D33C39F52" modified="2020-08-12" version="1">MAATNLTFDQIEEIREAFQVFDRENKGCITCKELGTVMRSLGQNPTEAELYDMIDEIDLDGDGTIDFSEFLYMMSQRMEDLGSDESLLLGFKIFDRDGNGYISTLELKTTMMMLGEKVTDEEVREIMAEIDQDHDGRISYAEFLSAMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V672</accession>
    <name>A0A6I8V672_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Steroidogenic acute regulatory protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Start1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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</entry>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">MV2-25</strain>
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                <consortium name="RefSeq"/>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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            <begin position="102"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_004444505.2"/>
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    <sequence length="137" mass="15626" checksum="218455792E22A65F" modified="2020-08-12" version="1">MIRLKWCCYCIALRKACIIIACLDFVINLTIATIGAKYQFIDRIEQAVAICHCIGCVFLIGGALLKSSVLLIFFLITSLTNYVILIAYVIYICFDWLPKGEFMVPIIITYTFVGIYFWLVVFSHYKNVTNDTVTIDV</sequence>
</entry>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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            <strain evidence="8">MV-25-SWS-2005</strain>
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        <text evidence="1">Belongs to the 5'-nucleotidase family.</text>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="5'-Nucleotdase_C"/>
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        <property type="entry name" value="Calcineurin-like_PHP"/>
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        <property type="entry name" value="5'-NUCLEOTIDASE"/>
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    </dbReference>
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        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
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        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_004444450.3"/>
        </source>
    </evidence>
    <sequence length="816" mass="88655" checksum="BCB69516B86796E2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9Q2</accession>
    <name>A0A6I8V9Q2_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
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    <dbReference type="RefSeq" id="XP_015036153.2">
        <property type="nucleotide sequence ID" value="XM_015180667.2"/>
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        <property type="component" value="Chromosome 4"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015036153.2"/>
        </source>
    </evidence>
    <sequence length="187" mass="22220" checksum="4AD05C548FCFF301" modified="2020-08-12" version="1">MGYPGSDYHMFWEFSLTTWNGLTTALDGNLQVIFLYMLAVGFMELIYNYKQIYLLLTMEDISEVSWPQTNWFTLSDRMLHHCWIASSLAMVALWNYLLYAFLWVKPELLYRWQVMYGTTIIADVLCLARARWAIRFGVGIAYLLPLWHIFCVNSLRTYTEQQAINNDSTYFYTRLFHIPSFLLQLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9S2</accession>
    <name>A0A6I8V9S2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Acetylcholinesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12 13" type="primary">Gli</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
            <tissue evidence="9 10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356170.3">
        <property type="nucleotide sequence ID" value="XM_001356134.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036759.2">
        <property type="nucleotide sequence ID" value="XM_015181273.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036760.2">
        <property type="nucleotide sequence ID" value="XM_015181274.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036761.2">
        <property type="nucleotide sequence ID" value="XM_015181275.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236860.1">
        <property type="nucleotide sequence ID" value="XM_033380969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9S2"/>
    <dbReference type="KEGG" id="dpo:4816652"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V9S2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000156">
        <property type="entry name" value="Neuroligin-4, Y-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634489" evidence="6">
        <location>
            <begin position="32"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="727"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="175"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="795"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="795"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="818"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="885"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="908"/>
            <end position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356170.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015036759.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015036760.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015036761.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033236860.1"/>
        </source>
    </evidence>
    <sequence length="949" mass="107966" checksum="2C3DD56FDBCE3BA5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAJ3</accession>
    <name>A0A6I8VAJ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">uncharacterized protein LOC4801387</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4801387</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <strain evidence="2 3">MV2-25</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358488.1">
        <property type="nucleotide sequence ID" value="XM_001358451.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037215.1">
        <property type="nucleotide sequence ID" value="XM_015181729.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAJ3"/>
    <dbReference type="GeneID" id="4801387"/>
    <dbReference type="KEGG" id="dpo:4801387"/>
    <dbReference type="OMA" id="RMPYICS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071197">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39948">
        <property type="entry name" value="GEO11419P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39948:SF1">
        <property type="entry name" value="GEO11419P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015037215.1"/>
        </source>
    </evidence>
    <sequence length="70" mass="8143" checksum="1962CAB784E7253E" modified="2020-08-12" version="1">MVNPLWFVFWLLVFWFVSFFVAFFCAFFYIWVYAFASCIPALSGIADMLLQGVQFPFFCGKAMLEGKPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAY9</accession>
    <name>A0A6I8VAY9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Orp8</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037425.2">
        <property type="nucleotide sequence ID" value="XM_015181939.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAY9"/>
    <dbReference type="FunCoup" id="A0A6I8VAY9">
        <property type="interactions" value="1219"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VAY9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VAY9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13286">
        <property type="entry name" value="PH_OPR5_ORP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000002">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000030">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000004">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1109"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="235"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="482"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="999"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1049"/>
            <end position="1083"/>
        </location>
    </feature>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015037425.2"/>
        </source>
    </evidence>
    <sequence length="1127" mass="123217" checksum="73C7949579ED4E49" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFE3</accession>
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        <name evidence="10" type="primary">retn</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
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                <consortium name="RefSeq"/>
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            <strain evidence="10">MV-25-SWS-2005</strain>
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</entry>
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    <accession>A0A6I8VGG8</accession>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <lineage>
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            <taxon>Muscomorpha</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="23126" checksum="EAF6EF1C3BB293E3" modified="2020-08-12" version="1">MAADEKEDISNTFRCFVSTAIGAMKLLPRLLAVQLLSILLIGKCVGYVRLTNLKCESLDPAFMEFPTCRLNVLGRGIIGGNIHLKLLKVPITKMNLRFNVYRRLSGYHPFLFNISTELCRFMKNPNPLQVFYYFHSGFLPYSNVNHTCPYTDDVFIRNCTLNDEMFKRVPLPKGNYMLTLAMDNGARKRVGIISIYIEIDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VH90</accession>
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            <taxon>Diptera</taxon>
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            <strain evidence="2 3">MV-25-SWS-2005</strain>
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        </source>
    </evidence>
    <sequence length="191" mass="22432" checksum="9320A11840AFDE51" modified="2020-08-12" version="1">MSLMRVGSRVTALGQKIGEKIVLPERFKDTFVEKWVKYWKGLVRDYSEVALGVVKESYTKPKKALAYGTGILCLYQMAQHNPDEGVFMTQLRRDNSRMIMVPPELQNHVSAKHLLMLERAINQKKLRFLSLGICTLVWVDLFSEDDCTYPAICEFTNVGFLNFHERVIDVGFWNEFWRLKWKMHNYDVNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIH5</accession>
    <name>A0A6I8VIH5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042058.2">
        <property type="nucleotide sequence ID" value="XM_015186572.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VIH5"/>
    <dbReference type="KEGG" id="dpo:26533018"/>
    <dbReference type="InParanoid" id="A0A6I8VIH5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015042058.2"/>
        </source>
    </evidence>
    <sequence length="204" mass="22934" checksum="78BC0EAB1F59BC64" modified="2020-08-12" version="1">MFECTRCRTRKGTLSSEPEFELVGPKIGPDGRHIKGVSDICHVCKCLTFFVKLGAEDRSRRVSVFEFHGIAPPPLPPRSLSQRKQRLEESGGEKHRPCQRNLYTPRTRSKSMACGTVSRLANCFKMSQQVSCGLFSTETTGPKSSDIVSTKKTSEGSSLSSPNIDKGKDEKEKRQLMKKLEKIRRRFEGLEVLESPKKSEEPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKL7</accession>
    <name>A0A6I8VKL7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">HBS1-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HBS1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015042916.2">
        <property type="nucleotide sequence ID" value="XM_015187430.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VKL7"/>
    <dbReference type="FunCoup" id="A0A6I8VKL7">
        <property type="interactions" value="1688"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900553"/>
    <dbReference type="InParanoid" id="A0A6I8VKL7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VKL7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16267">
        <property type="entry name" value="HBS1-like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04093">
        <property type="entry name" value="HBS1_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000035">
        <property type="entry name" value="HBS1-like translational GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015033">
        <property type="entry name" value="HBS1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037189">
        <property type="entry name" value="HBS1-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08938">
        <property type="entry name" value="HBS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109732">
        <property type="entry name" value="HBS1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="224"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="38"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015042916.2"/>
        </source>
    </evidence>
    <sequence length="649" mass="71964" checksum="AC842BB1ED9E9662" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLE7</accession>
    <name>A0A6I8VLE7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">Major facilitator superfamily domain-containing protein 8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12 13 14 15 16" type="primary">Cln7</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11 12">MV-25-SWS-2005</strain>
            <tissue evidence="10 11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002135441.2">
        <property type="nucleotide sequence ID" value="XM_002135405.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043766.2">
        <property type="nucleotide sequence ID" value="XM_015188280.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043767.2">
        <property type="nucleotide sequence ID" value="XM_015188281.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043768.2">
        <property type="nucleotide sequence ID" value="XM_015188282.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043769.2">
        <property type="nucleotide sequence ID" value="XM_015188283.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043771.2">
        <property type="nucleotide sequence ID" value="XM_015188285.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043772.2">
        <property type="nucleotide sequence ID" value="XM_015188286.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLE7"/>
    <dbReference type="KEGG" id="dpo:6900083"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VLE7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17326">
        <property type="entry name" value="MFS_MFSD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051068">
        <property type="entry name" value="MFS_Domain-Containing_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YAJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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    <sequence length="528" mass="58963" checksum="FA341B26C9C329D4" modified="2020-08-12" version="1"> </sequence>
</entry>
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</entry>
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        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1597"/>
            <end position="1608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1854"/>
            <end position="1948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1950"/>
            <end position="1960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033232346.1"/>
        </source>
    </evidence>
    <sequence length="2050" mass="215519" checksum="6BD0C0B9DBE4850B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VN37</accession>
    <name>A0A6I8VN37_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphatidylserine decarboxylase proenzyme, mitochondrial</fullName>
            <ecNumber evidence="13">4.1.1.65</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="13">Phosphatidylserine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="13">Phosphatidylserine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="16" type="primary">Pisd</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. May be involved in lipid droplet biogenesis at the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:20828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="4.1.1.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="13">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Phosphatidylserine decarboxylase alpha chain</molecule>
        <subcellularLocation>
            <location evidence="13">Mitochondrion inner membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Intermembrane side</orientation>
        </subcellularLocation>
        <text evidence="13">Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Phosphatidylserine decarboxylase beta chain</molecule>
        <subcellularLocation>
            <location evidence="13">Mitochondrion inner membrane</location>
            <topology evidence="13">Single-pass membrane protein</topology>
            <orientation evidence="13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="13">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type I sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="13"/>
    <dbReference type="RefSeq" id="XP_033232480.1">
        <property type="nucleotide sequence ID" value="XM_033376589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VN37"/>
    <dbReference type="FunCoup" id="A0A6I8VN37">
        <property type="interactions" value="1688"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801761"/>
    <dbReference type="InParanoid" id="A0A6I8VN37"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016540">
        <property type="term" value="P:protein autoprocessing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03208">
        <property type="entry name" value="PS_decarb_PSD_B_type1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033661">
        <property type="entry name" value="PSD_type1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF6">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11 13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5026405028" description="Phosphatidylserine decarboxylase beta chain" evidence="13">
        <location>
            <begin position="1"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026405027" description="Phosphatidylserine decarboxylase alpha chain" evidence="13">
        <location>
            <begin position="414"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial matrix" evidence="13">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial intermembrane" evidence="13">
        <location>
            <begin position="126"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="13">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="13">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="13">
        <location>
            <position position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity" evidence="13">
        <location>
            <position position="414"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="13">
        <location>
            <begin position="413"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="13">
        <location>
            <position position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033232480.1"/>
        </source>
    </evidence>
    <sequence length="449" mass="50911" checksum="C7112D00A90C5FE3" modified="2020-08-12" version="1">MVSYFVPRGRVLLKAGNLRKVVQPHQPQQLQLQAAKPTQQQNGSWPVVRHLRHFSSNSSSGNAPLHRLKPQTQQQPQPRKQQLNHNQNLAQVRRNVLSRWTGFLLRWAPMGICVFGAIEWQFHKKRCERDGSPRTASEFQSRVYCSLPLRIISRCWGWLAACYMPTTLRPYVYGWYSSAFNVNLSEAQYPEYEHYNSLAEFFTRPLKEGARTIDANAPLVSPADGKVLHFGSASDSLIEQVKGVNYSIEDFLGPLQTLEEAKTGVSYAQALKRQRDGTTELYQCVIYLAPGDYHRFHSPTAWQPTVRRHFSGELLSVSPKVAGWLPGLFCLNERVLYMGQWKHGFFSYTAVGATNVGSVEIYMDAELKTNRWTGLNVGKHPPSTYEYDEVALDAEQKPKELGKGDLIGQFNMGSTIVLLFEAPKNFKFDIVAGQKIHVGESLGHIVERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNQ4</accession>
    <name>A0A6I8VNQ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232700.1">
        <property type="nucleotide sequence ID" value="XM_033376809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNQ4"/>
    <dbReference type="KEGG" id="dpo:117183202"/>
    <dbReference type="InParanoid" id="A0A6I8VNQ4"/>
    <dbReference type="OMA" id="CRIQTAA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VNQ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033232700.1"/>
        </source>
    </evidence>
    <sequence length="68" mass="7888" checksum="A3C74CE90E3D7E9C" modified="2020-08-12" version="1">MRYLHDLNVQISQNRRSVSVSKQLDRDLCNQHFDTFETFWGRPGHGARGDHINKLKLDNLLYGASETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VP30</accession>
    <name>A0A6I8VP30_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">LOW QUALITY PROTEIN: uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC117183337</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_033376924.1"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta-prop_FAM234A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045232">
        <property type="entry name" value="FAM234"/>
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    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR21419">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419:SF30">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrin alpha N-terminal domain"/>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAM234A/B beta-propeller" evidence="6">
        <location>
            <begin position="142"/>
            <end position="474"/>
        </location>
    </feature>
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        <location>
            <begin position="602"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="608"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033232815.1"/>
        </source>
    </evidence>
    <sequence length="773" mass="85155" checksum="BE978C08FA2C463D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VP98</accession>
    <name>A0A6I8VP98_DROPS</name>
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        <submittedName>
            <fullName evidence="11">Homeobox protein HMX3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">lbe</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor LBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="LADYBIRD EARLY-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="HTHREPRESSR"/>
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    </dbReference>
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        <location>
            <begin position="380"/>
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        </location>
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        </location>
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            <begin position="1"/>
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        </location>
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        </location>
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        </location>
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        </location>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033232599.1"/>
        </source>
    </evidence>
    <sequence length="511" mass="54350" checksum="6A84A4F1F4D6EF2D" modified="2020-08-12" version="1">MLCPPTMRPASPAESEISVGGAPSPLPTQLQHQHQPHPHAHQLHHPRASTIDLLQQQQFLMQHHAAAAAAAAAGLTRGAVGSLPEDYFQPLKRLRMSSSSSEPREHTPSPPALAPAPTAADSQPQTTTKAAIEGVKSFSIADILGHSQKQRSDSVSPPPAATLLAPPANRATAGLLQPRTEPLEAHHAAAAMLLPGGQIVRPWDHLLGPMPVRPFIPSALLHYEQRLALDYHRQLQEHFNAQAQLLRHMGMDIIPSESGSDRSSSAASDCCSPEISRDSAAAAAAAAARRSHSPQSSAQVTAERERHHSAGSASASSPSQSSGGPSASSGGNAAAVAAAAAKAKANGTPLDALFQMTTKDFDESQDKSHLDIFSNRPQPKKKRKSRTAFTNHQIFELEKRFLYQKYLSPADRDEIAASLGLSNAQVITWFQNRRAKQKRDIEELKKDFDSVKVFSAHKSFLENVNDLSILKKKPMHEADMVGLAAAAAAAGMVTVQVPGSAASSLGGAPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTS6</accession>
    <name>A0A6I8VTS6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AIMP1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033234481.1">
        <property type="nucleotide sequence ID" value="XM_033378590.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8VTS6">
        <property type="interactions" value="1971"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VTS6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000047">
        <property type="entry name" value="tyrosine--tRNA ligase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF33">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="9">
        <location>
            <begin position="159"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033234481.1"/>
        </source>
    </evidence>
    <sequence length="320" mass="34426" checksum="AC12A4950AFF9C2A" modified="2020-08-12" version="1">MLRITSIVSRRYGIGIYFENHFSKKMAALQQIVQNNERASALIESLKSEMAGIQSQLVLRKKQELIKENAALEKEIEAALAQLVQLELRNGKQQILVPGSREFCTDAAAEAGSKPAAAAPSNGPASEAKAPSAPQKEKKVKEKKPAAAKPSPVAEAPVDVGRLDLRVGKIIEVGRHPDADSLYLEKIDCGEAAPRTVVSGLVKFVPLEEMQNRMVVVMCNLKPAKMRGVTSEAMVMCASTPDKVEVLSPPVGAVPGDLVHCEGYTRQPDAQLNPKKKIFETCAPDLMTNADLVACYKGSALHVPGKGNVVSQSLKNVNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VUS1</accession>
    <name>A0A6I8VUS1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Kinesin-like protein KIF13A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Khc-73</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033234796.1">
        <property type="nucleotide sequence ID" value="XM_033378905.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VUS1">
        <property type="interactions" value="441"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804863"/>
    <dbReference type="InParanoid" id="A0A6I8VUS1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VUS1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22706">
        <property type="entry name" value="FHA_KIF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000002">
        <property type="entry name" value="Kinesin family member 13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000010">
        <property type="entry name" value="Kinesin family member 13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000014">
        <property type="entry name" value="Uncharacterized protein, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="5"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="14">
        <location>
            <begin position="1865"/>
            <end position="1907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="771"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="843"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1425"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1506"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1623"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1770"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1922"/>
            <end position="1956"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="589"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1506"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1516"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1539"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1647"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1692"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1703"/>
            <end position="1713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1714"/>
            <end position="1724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1731"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1780"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1929"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="100"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033234796.1"/>
        </source>
    </evidence>
    <sequence length="1956" mass="218934" checksum="35A920D701097A1C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWW2</accession>
    <name>A0A6I8VWW2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Accessory gland protein Acp32CD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6 7" type="primary">Acp32CD</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
            <tissue evidence="4 5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132747.3">
        <property type="nucleotide sequence ID" value="XM_002132711.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235575.1">
        <property type="nucleotide sequence ID" value="XM_033379684.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235576.1">
        <property type="nucleotide sequence ID" value="XM_033379685.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235577.1">
        <property type="nucleotide sequence ID" value="XM_033379686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWW2"/>
    <dbReference type="KEGG" id="dpo:4817438"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243566">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634578" evidence="2">
        <location>
            <begin position="25"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002132747.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033235577.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="29956" checksum="A8AAF7396052F93B" modified="2020-08-12" version="1">MWRIRLLTLVLLTLVQMEATRVLGQKYYMNFAFNNNNPDAALSGEGNGGGGGGADGRTSPNEQYNDHGIGDEGDHDADNLSYHNADGGDDNGDHNEGPQGDHGGDHDADYVGGDHDGGDGGDGGDGGDGGDGGDDGGGGDGLQDDYVDNGDANDHGGDGGMDNGDNGEGIPDPIPGVDDDDDSDSKDAHNRRQKLAEIEAEGGAAANGAHSHHSSYEISIDDSFGGRYVRSIYESSESHGHSGSRGGSGSGGKADSSDSQGGGGGGGAAAGRARKAPKAPKAKERTYGIDDYEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWX7</accession>
    <name>A0A6I8VWX7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235587.1">
        <property type="nucleotide sequence ID" value="XM_033379696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWX7"/>
    <dbReference type="KEGG" id="dpo:6903282"/>
    <dbReference type="InParanoid" id="A0A6I8VWX7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235587.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="24918" checksum="4B51218E7336ECE9" modified="2020-08-12" version="1">MNFGVVRMLDGVRRRVLGHEFNECETLVLEDTHILNVPKRWPSRRSSRCERRSCYWSRASTSPSPSLRRSSRQHSPSIADTPQKSPAPRWVLYERPAWTAKYFGVQETFKSTTTAIKTQSMPPRIVTKAEATNEHVRQLGTTFLTQKRREMLKRMSPISLDTYTSSSISPTAIPSPKRAHSPGNRAKATISSFEDIEVTPQELNAAGKDFKRLCVEILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXY7</accession>
    <name>A0A6I8VXY7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acanthoscurrin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117183933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235429.1">
        <property type="nucleotide sequence ID" value="XM_033379538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXY7"/>
    <dbReference type="KEGG" id="dpo:117183933"/>
    <dbReference type="InParanoid" id="A0A6I8VXY7"/>
    <dbReference type="OMA" id="NANAQSF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026355136" evidence="2">
        <location>
            <begin position="17"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235429.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="14628" checksum="AE5CD978D3483144" modified="2020-08-12" version="1">MKLFIALSVLVAVASALPQFGGGNANANANANAQAQGFGGGGGFGGRPGGFGGGPGFGGGGFGGRPGGGFGGGPGFGGGPGFGGGPGFGGGPGFGGGGFGGGPGFGGGGFGGRPGFGGGGGGFGGRPGGGFGGGGGGGSSSASASSSASAAGGGRRGGGGSASASSSASAAGGGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VY62</accession>
    <name>A0A6I8VY62_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Cth</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_033235469.1">
        <property type="nucleotide sequence ID" value="XM_033379578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VY62"/>
    <dbReference type="FunCoup" id="A0A6I8VY62">
        <property type="interactions" value="728"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899855"/>
    <dbReference type="InParanoid" id="A0A6I8VY62"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00202"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248970">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VY62">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000181">
        <property type="entry name" value="cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033235469.1"/>
        </source>
    </evidence>
    <sequence length="393" mass="43080" checksum="0E343DE351338844" modified="2020-08-12" version="1">MSFKIQPNGFDTKAIHAGQSPEQWKSACVIPPISLSTTFKQDAPGEHRGYEYSRSGNPTRNVLETCLAALDNAKYGLTFSSGLGATTAVLTMLKSGDHIIVGDDVYGGTNRLVRHVASRLGISATFVDPTNLESVKAAIQPVTKLIWIESPTNPLIKVADIKEIATICHDIRDDLILAVDNTFLTSYFQRPLELGADLVCYSLTKYMNGHTDVVMGGITMNCDNLYDSLKFLQNAVGIVPSPFDCYQVNRSLKTLSLRMKQHQHNAIKIAKFLEAHEFVDKVLHPALPSHPQHQVALKQTYGYSGVFSFYIKGELKHSTAFLKALKLFTLAESLGGYESLAELPSIMTHASVPIEDRKSLGISDALIRLSVGLEDSDDLINDLSLALEIASKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZJ0</accession>
    <name>A0A6I8VZJ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC117184035</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033235929.1">
        <property type="nucleotide sequence ID" value="XM_033380038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZJ0"/>
    <dbReference type="FunCoup" id="A0A6I8VZJ0">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184035"/>
    <dbReference type="InParanoid" id="A0A6I8VZJ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VZJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1112">
        <property type="entry name" value="COL_CUTICLE_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026287288" description="SCP domain-containing protein" evidence="4">
        <location>
            <begin position="30"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="5">
        <location>
            <begin position="71"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="266"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="328"/>
            <end position="337"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="346"/>
            <end position="356"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="358"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="376"/>
            <end position="418"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="427"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033235929.1"/>
        </source>
    </evidence>
    <sequence length="532" mass="57560" checksum="6C362F816F918CAC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0D0</accession>
    <name>A0A6I8W0D0_DROPS</name>
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        <submittedName>
            <fullName evidence="4 5 6">Embryonic polarity protein dorsal isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">Dif</name>
    </gene>
    <organism evidence="3 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
            <tissue evidence="4 5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035983.2">
        <property type="nucleotide sequence ID" value="XM_015180497.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035984.2">
        <property type="nucleotide sequence ID" value="XM_015180498.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236214.1">
        <property type="nucleotide sequence ID" value="XM_033380323.1"/>
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    <dbReference type="KEGG" id="dpo:4817470"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W0D0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005654">
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0033554">
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    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:peripheral nervous system neuron development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002225">
        <property type="term" value="P:positive regulation of antimicrobial peptide production"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_E-set"/>
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    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
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    <dbReference type="InterPro" id="IPR033926">
        <property type="entry name" value="IPT_NFkappaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
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    <dbReference type="InterPro" id="IPR008967">
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        <property type="entry name" value="RHD_CS"/>
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        <property type="entry name" value="RHD_dimer"/>
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        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF25">
        <property type="entry name" value="DORSAL-RELATED IMMUNITY FACTOR DIF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00057">
        <property type="entry name" value="NFKBTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="608"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="952"/>
            <end position="980"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="749"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="821"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="963"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015035983.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015035984.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033236214.1"/>
        </source>
    </evidence>
    <sequence length="998" mass="109112" checksum="EB9584E534B1A117" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3Z2</accession>
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein Ir67a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ir67a</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_033381523.1"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
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        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF41">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="566"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_033237414.1"/>
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    <sequence length="604" mass="69431" checksum="4A931901DAF23EA3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6B5</accession>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein inaF-D</fullName>
        </submittedName>
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        <name evidence="4" type="primary">inaF-D</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238219.1">
        <property type="nucleotide sequence ID" value="XM_033382328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6B5"/>
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    </dbReference>
    <dbReference type="KEGG" id="dpo:117184488"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="entry name" value="InaF-motif"/>
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        <property type="entry name" value="INAF MOTIF CONTAINING 2"/>
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        <property type="entry name" value="ZGC:153157"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="59"/>
        </location>
    </feature>
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            <begin position="165"/>
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    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="RefSeq" id="XP_033238219.1"/>
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    <sequence length="371" mass="40969" checksum="503A432232CC3942" modified="2020-08-12" version="1">MNSKVNSLEEKVKLPPTETISRCYQPQQSNRKVIRILTVAVYVLCVSLAAIMLSLYYIFIWDPTIRPFVTKANHCDKIVIPEKIAIRLLNSSEVTAEQFYKHILEQYRIHNHLQQQRQQFLQKQHLQLQQLEASNRFQEIFATATIIQAHPHPNPREPLKKPLVVPSANNSSSGSISDLGAFRMEPPAPQPSNMEEMGKGATTGHHFPMLLDTSPPADSSGIGHLKRKTHRGHYKHHRARAGGLKKLPSSSGLGSSSISSSATASTTSAGEQLAATAAATVPHGYMDTPLNPAAGTIVQQPQLQLYTSMPIPLILSPSEEKRPSHHAHNHAHGERRGGAQARKRTTTASVSGYDSQTYLNPFLTGELIFEK</sequence>
</entry>
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    <accession>A0A6I8WAG5</accession>
    <name>A0A6I8WAG5_DROPS</name>
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        <recommendedName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Cht6</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="18">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
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        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
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    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
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        <property type="nucleotide sequence ID" value="XM_033384497.1"/>
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        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WAG5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:endochitinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:chitin catabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="CDD" id="cd02872">
        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acidic mammalian chitinase"/>
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    </dbReference>
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        <property type="entry name" value="Acidic mammalian chitinase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00636">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH18_1"/>
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    </dbReference>
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        <property type="entry name" value="GH18_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="4" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5026186336" description="chitinase" evidence="14">
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        </location>
    </feature>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF237">
        <property type="entry name" value="PICKPOCKET 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033240968.1"/>
        </source>
    </evidence>
    <sequence length="192" mass="22140" checksum="1B0BCF40481E6459" modified="2020-08-12" version="1">MRMMIPSDSETHLMVRAFSTRFTANLAGLPIEDRRCFLPDEHRLWFYSAYSLSNCLSECRSKKIFQSCGCVPPHAPGESTWPICGLQQVECVRKYDEISEQITAVERDCNCLPPCTFHRYEFESDVSLIKGSNSTNEVRLNVYYDLAFTEELLLEVYETWLSFIGKCPRAYSINETTNKTSAPRIKVSFIYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDM2</accession>
    <name>A0A6I8WDM2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66</fullName>
            <ecNumber evidence="4">1.14.11.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Histone lysine demethylase NO66</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC26533390</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:42032"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ROX family. NO66 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_033241488.1">
        <property type="nucleotide sequence ID" value="XM_033385597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDM2"/>
    <dbReference type="FunCoup" id="A0A6I8WDM2">
        <property type="interactions" value="1952"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WDM2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WDM2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140680">
        <property type="term" value="F:histone H3K36me/H3K36me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032453">
        <property type="term" value="F:histone H3K4 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045471">
        <property type="term" value="P:response to ethanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000013">
        <property type="entry name" value="Ribosomal oxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1500:FF:000001">
        <property type="entry name" value="ribosomal oxygenase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.40:FF:000001">
        <property type="entry name" value="ribosomal oxygenase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1500">
        <property type="entry name" value="JmjC domain-containing ribosomal oxygenase (ROX), dimer domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039994">
        <property type="entry name" value="NO66-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049043">
        <property type="entry name" value="WHD_RIOX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13096">
        <property type="entry name" value="MINA53 MYC INDUCED NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13096:SF8">
        <property type="entry name" value="RIBOSOMAL OXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08007">
        <property type="entry name" value="JmjC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21233">
        <property type="entry name" value="WHD_RIOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="19">
        <location>
            <begin position="697"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="15"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="449"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="467"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_033241488.1"/>
        </source>
    </evidence>
    <sequence length="1037" mass="114471" checksum="99E766DF30963D0F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDV2</accession>
    <name>A0A6I8WDV2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein Lilipod isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lili</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LIMR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241595.1">
        <property type="nucleotide sequence ID" value="XM_033385704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDV2"/>
    <dbReference type="FunCoup" id="A0A6I8WDV2">
        <property type="interactions" value="936"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800244"/>
    <dbReference type="InParanoid" id="A0A6I8WDV2"/>
    <dbReference type="OMA" id="QQRRTWW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079141">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008075">
        <property type="entry name" value="LIMR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12625">
        <property type="entry name" value="LIPOCALIN-1 INTERACTING MEMBRANE RECEPTOR LIMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12625:SF0">
        <property type="entry name" value="PROTEIN LILIPOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01692">
        <property type="entry name" value="LIPOCALINIMR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033241595.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="59948" checksum="503F785D6F186FCD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEK8</accession>
    <name>A0A6I8WEK8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Transcription factor Ouib-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC13036406</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241860.1">
        <property type="nucleotide sequence ID" value="XM_033385969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEK8"/>
    <dbReference type="FunCoup" id="A0A6I8WEK8">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:13036406"/>
    <dbReference type="InParanoid" id="A0A6I8WEK8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000325">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001156">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF57">
        <property type="entry name" value="ZINC FINGER PROTEIN 154-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="15">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="272"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="133"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="184"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033241860.1"/>
        </source>
    </evidence>
    <sequence length="403" mass="46582" checksum="46CCFB363FCB6B3C" modified="2020-08-12" version="1">MALPCRTCGEHAVNPRKLFDENGTDILNNLLKLTGIWVGMTPCKTFKLTYLTNRTGFPSHICASCLQHSNDAMAFRELCIRTNKLWYISADSKPTDQPLNLDPLMEYDEAAIPEENNEYAKVQIETVWKERNNSSPLSHLTSSQHHREPYTRRKKAKEQSEKIDNNGEFQDELLDFDPLLNEDAGVVPKEENPDEVKDKGSSLSSRKSRLDSSSVPKKGLLRGSRKSNIDPLAKKHRYRSIRGFYCDQCGKCFKDKSNLNVHLKRHTGVKQFECEECGRKEFTMHLLSLHMRIKHNGELPYSCKHCGQGFDNCTKRLRHERTTHNECPDTRAYVCHVCRKAFKRKLSLKRHELVHTGEQPFHCETCHVSFNRKSSLRTHNRSMLHIKKVEQESQINEIMDEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>B5DMH2</accession>
    <accession>A0A6I8V087</accession>
    <name>B5DMH2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein cona</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cona</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134065.2">
        <property type="nucleotide sequence ID" value="XM_002134029.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DMH2"/>
    <dbReference type="FunCoup" id="B5DMH2">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DMH2"/>
    <dbReference type="GeneID" id="6901377"/>
    <dbReference type="KEGG" id="dpo:6901377"/>
    <dbReference type="HOGENOM" id="CLU_1327606_0_0_1"/>
    <dbReference type="InParanoid" id="B5DMH2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248220">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DMH2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002134065.2"/>
        </source>
    </evidence>
    <sequence length="221" mass="24981" checksum="BEFE2E3E858C7F4D" modified="2016-01-20" version="2">MHSDNDNACALNGNQQDSKEVACDPVSTIQAYLAEMLRQTRAENTSLENELNELQENQKLLTDQKAEAICSMSLPIAGENSIELLPNLDELTAKETTLSSQLSDLVKIKQQLSLAYADHCQQAIHVTEERDGNVTYDEFTSMSYEDLKKLIQEDSIKLCEMKRKSEELTEILRSKRKEQDANHKALLEQISVLELIALEAFELANEVKVDLSYCNEGRQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPX1</accession>
    <accession>A0A6I8V0S3</accession>
    <name>B5DPX1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyrimidodiazepine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GstO3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Omega family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135068.1">
        <property type="nucleotide sequence ID" value="XM_002135032.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPX1"/>
    <dbReference type="SMR" id="B5DPX1"/>
    <dbReference type="FunCoup" id="B5DPX1">
        <property type="interactions" value="397"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DPX1"/>
    <dbReference type="GeneID" id="6900705"/>
    <dbReference type="KEGG" id="dpo:6900705"/>
    <dbReference type="eggNOG" id="KOG0406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_9_2_1"/>
    <dbReference type="InParanoid" id="B5DPX1"/>
    <dbReference type="OMA" id="LDDTYPG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245243">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DPX1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045174">
        <property type="term" value="F:glutathione dehydrogenase (ascorbate) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03184">
        <property type="entry name" value="GST_C_Omega"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000009">
        <property type="entry name" value="Glutathione S-transferase omega-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000123">
        <property type="entry name" value="Glutathione transferase o1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005442">
        <property type="entry name" value="GST_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01625">
        <property type="entry name" value="GSTRNSFRASEO"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002135068.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="27722" checksum="86D8C9F6E0724D2B" modified="2008-10-14" version="1">MSSGKHLGKGSSKPVLPDDGKLRLFSMRFCPYAERAHLVLNAKKVPYHTVYINLTEKPEWLVEVSPLLKVPALQLPGEKGEPSLIESLIIAEYLDEKYPQNPLLPKDPLKRAEDKILLERFSAVTNAFMKILFQSTGLDDYWLALEIFEKELVKRGTPFFSGDRPGFVDYMIWPWLERLAVIGFILEDSYNFDAKRFPRIAKLIDLLKADDVVKSFYATPEQHIEFWRGRKSGNTNYDLLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q297K8</accession>
    <name>JAGN_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein jagunal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">jagn</name>
        <name type="ORF">GA10683</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for endoplasmic reticulum organization and proper vesicular traffic during vitellogenesis. Required for oocyte and bristle growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the jagunal family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL28197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q297K8"/>
    <dbReference type="SMR" id="Q297K8"/>
    <dbReference type="FunCoup" id="Q297K8">
        <property type="interactions" value="1044"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q297K8"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0285739">
        <property type="protein sequence ID" value="FBpp0284177"/>
        <property type="gene ID" value="FBgn0070739"/>
    </dbReference>
    <dbReference type="GeneID" id="4802061"/>
    <dbReference type="KEGG" id="dpo:4802061"/>
    <dbReference type="CTD" id="40714"/>
    <dbReference type="eggNOG" id="KOG4054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121621_0_0_1"/>
    <dbReference type="InParanoid" id="Q297K8"/>
    <dbReference type="OMA" id="PYGVLWY"/>
    <dbReference type="PhylomeDB" id="Q297K8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070739">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q297K8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022416">
        <property type="term" value="P:chaeta development"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001555">
        <property type="term" value="P:oocyte growth"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009787">
        <property type="entry name" value="Jagunal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955:SF1">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07086">
        <property type="entry name" value="Jagunal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0221">Differentiation</keyword>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0896">Oogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000313616" description="Protein jagunal">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q7K1V5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="197" mass="23057" checksum="2F78004E663D0E59" modified="2006-04-04" version="1">MATRGGPMVAGTDGNDFEFRQRVAGTYQISLLNKSRLKYCIFFHALLFFVMLAKLTSDILDRLDIFVLEIEELEVPSPLWWEYVWAGSLLTSFLGLSAARGNKVREMQKYMIAILVFAILPLLYCFAYYFSDVWEFATMDKSVELDETDIFIWRGYPYGVFWYAFCFVGFQVHGFTLYFAYNLVKVWKARTATRKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D23</accession>
    <accession>A0A6I8UBZ5</accession>
    <name>Q29D23_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Acbp5</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353090.1">
        <property type="nucleotide sequence ID" value="XM_001353054.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D23"/>
    <dbReference type="SMR" id="Q29D23"/>
    <dbReference type="STRING" id="46245.Q29D23"/>
    <dbReference type="GeneID" id="4812711"/>
    <dbReference type="KEGG" id="dpo:4812711"/>
    <dbReference type="eggNOG" id="KOG0817">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118853_4_1_1"/>
    <dbReference type="InParanoid" id="Q29D23"/>
    <dbReference type="OMA" id="KYAPIYT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079139">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29D23">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF137">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 3, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353090.1"/>
        </source>
    </evidence>
    <sequence length="81" mass="9098" checksum="A6D33CCA6D28AF17" modified="2006-04-04" version="1">MDFNAILEKTKAFSKKPPTEVYLEFYGLYKQIHEGDIAIEKPADAEGAAKYDAWLSRKGLSVDAAKAAYTALYEKYNPIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EH3</accession>
    <accession>A0A6I8UAW4</accession>
    <name>Q29EH3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Survival motor neuron protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Smn</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352590.1">
        <property type="nucleotide sequence ID" value="XM_001352554.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EH3"/>
    <dbReference type="SMR" id="Q29EH3"/>
    <dbReference type="FunCoup" id="Q29EH3">
        <property type="interactions" value="819"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EH3"/>
    <dbReference type="GeneID" id="4812177"/>
    <dbReference type="KEGG" id="dpo:4812177"/>
    <dbReference type="eggNOG" id="KOG4327">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077852_0_0_1"/>
    <dbReference type="InParanoid" id="Q29EH3"/>
    <dbReference type="OMA" id="MSMLTAW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074138">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EH3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22852">
        <property type="entry name" value="SMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20398">
        <property type="entry name" value="Tudor_SMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040424">
        <property type="entry name" value="Smn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047313">
        <property type="entry name" value="SMN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049481">
        <property type="entry name" value="SMN_G2-BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047298">
        <property type="entry name" value="Tudor_SMN_eumet"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39267:SF1">
        <property type="entry name" value="SURVIVAL MOTOR NEURON PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39267">
        <property type="entry name" value="SURVIVAL MOTOR NEURON-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20636">
        <property type="entry name" value="SMN_G2-BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20635">
        <property type="entry name" value="SMN_YG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352590.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="24906" checksum="9B7E955ADEEC5538" modified="2006-04-04" version="1">MCDETNSAAWDDSLLIKVYDESVNLAREALARRLADSTNTREEENKMDEVNTATNATSPEPVLFKVGDFARATFTDGVDYEGTVVSINEEAATCVIRYLGYENEQEILVAELLPSWGKDARRQQSLMAYQFEVETAHQEKARGKSASKKSSVKTKPAASAGLSGGPCMPTMPLIPPIIPPYGNPGEEQDLMSMLTAWYMSGYYTGYFQGKKAIPRQVEKKKTPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FD9</accession>
    <accession>A0A6I8UES4</accession>
    <name>Q29FD9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mRpL36</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354263.1">
        <property type="nucleotide sequence ID" value="XM_001354227.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FD9"/>
    <dbReference type="SMR" id="Q29FD9"/>
    <dbReference type="FunCoup" id="Q29FD9">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FD9"/>
    <dbReference type="GeneID" id="4814169"/>
    <dbReference type="KEGG" id="dpo:4814169"/>
    <dbReference type="eggNOG" id="KOG4122">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154068_1_0_1"/>
    <dbReference type="InParanoid" id="Q29FD9"/>
    <dbReference type="OMA" id="QNTTRLW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075096">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FD9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052143">
        <property type="entry name" value="Mitoribosomal_bL36m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_bL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_bL36_sp"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46909">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L36, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46909:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL36M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="Ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354263.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="14439" checksum="0C86D34F652D1F28" modified="2006-04-04" version="1">MSFAGKILQHSARLWNGLSSKRGFHVLMRPTAIAAGVTNNSNQTQLVANTNVSAGLLTPVSTLLQQVAGFKVKGRLKRRCKDCYIVVRQERGYVICPTHPRHKQMSMKKRDYKSWILTHATQSKERGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FP7</accession>
    <accession>A0A6I8UF15</accession>
    <name>Q29FP7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Monocyte to macrophage differentiation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4814241</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354479.2">
        <property type="nucleotide sequence ID" value="XM_001354443.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FP7"/>
    <dbReference type="FunCoup" id="Q29FP7">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FP7"/>
    <dbReference type="GeneID" id="4814241"/>
    <dbReference type="KEGG" id="dpo:4814241"/>
    <dbReference type="eggNOG" id="KOG4243">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051078_0_1_1"/>
    <dbReference type="InParanoid" id="Q29FP7"/>
    <dbReference type="OMA" id="LIPMAHA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078308">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FP7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140911">
        <property type="term" value="F:pore-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005744">
        <property type="entry name" value="Hy-lIII"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01065">
        <property type="entry name" value="hlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF3">
        <property type="entry name" value="LD03007P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354479.2"/>
        </source>
    </evidence>
    <sequence length="301" mass="34305" checksum="49DD357AE0AC14BA" modified="2008-10-14" version="2">MSTIGIGNNGNGNGSVSLMQDIQNKYSFLENLFSKFWKSIIKSNSSLKLQLRNIKWKNAKAKPGCAYQPTEIEQMANVITHGMWILPALFATIKLFERSTNASQYLVSWVYGGALCMLFTISTFFHCSCYCAEHKPPRNYKAWPAFGWRTYQGLKNVLHRCDRAMIYVFIAGSYFPWLTLENTDHSAILFSMEWVIWLMAGVGIAYQQLFHERYKCLETFFYLVMGLGPALVVVLTGHHFHGMIQLKYGGAFYILGIVFFKSDGFIPMAHAIWHLFVVLAAGCHYYAILVNLYPSDISISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GA7</accession>
    <accession>A0A6I8UGS4</accession>
    <name>Q29GA7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rho GTPase-activating protein gacK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Dok</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355146.2">
        <property type="nucleotide sequence ID" value="XM_001355110.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GA7"/>
    <dbReference type="SMR" id="Q29GA7"/>
    <dbReference type="FunCoup" id="Q29GA7">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GA7"/>
    <dbReference type="GeneID" id="4814814"/>
    <dbReference type="KEGG" id="dpo:4814814"/>
    <dbReference type="eggNOG" id="KOG4047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035187_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GA7"/>
    <dbReference type="OMA" id="MCESKED"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075251">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PH_domain"/>
    </dbReference>
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        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF62">
        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="602"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355146.2"/>
        </source>
    </evidence>
    <sequence length="656" mass="70571" checksum="D4F9493517BCFE97" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GM9</accession>
    <accession>A0A6I8UGA9</accession>
    <name>Q29GM9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable insulin-like peptide 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Ilp7</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355024.2">
        <property type="nucleotide sequence ID" value="XM_001354988.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GM9"/>
    <dbReference type="FunCoup" id="Q29GM9">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="GeneID" id="4814981"/>
    <dbReference type="KEGG" id="dpo:4814981"/>
    <dbReference type="eggNOG" id="ENOG502S96B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138138_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GM9"/>
    <dbReference type="OMA" id="KTGCTWE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072245">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04365">
        <property type="entry name" value="IlGF_relaxin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.100.10">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036438">
        <property type="entry name" value="Insulin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56994">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043590304" evidence="4">
        <location>
            <begin position="34"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001355024.2"/>
        </source>
    </evidence>
    <sequence length="164" mass="19202" checksum="92A740F30808213C" modified="2008-10-14" version="2">MGQPSTTGVGPRSWTLCGAVLLFILQLFQTSDALHHTEEGLELLFRERSQSDWENVWHQETHSRCRDKLIRQLYWACEKDIYRLTRRNRKRTGNDEAWIKQSSSSSSKPGTPPSTWLHVNYANMFLRTRRSNAPSITNECCTKAGCTWEEYAEYCPSNKRRNHY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-12-04" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HF1</accession>
    <name>NEUFC_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Neuferricin homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Cytochrome b5 domain-containing protein 2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GA11364</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Heme-binding protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residues at position 98 and 125.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EAL31807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HF1"/>
    <dbReference type="SMR" id="Q29HF1"/>
    <dbReference type="FunCoup" id="Q29HF1">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HF1"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0284386">
        <property type="protein sequence ID" value="FBpp0282824"/>
        <property type="gene ID" value="FBgn0071418"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814739"/>
    <dbReference type="eggNOG" id="KOG1108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065455_0_1_1"/>
    <dbReference type="InParanoid" id="Q29HF1"/>
    <dbReference type="OMA" id="PPCNIEW"/>
    <dbReference type="PhylomeDB" id="Q29HF1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071418">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF4">
        <property type="entry name" value="NEUFERRICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000312329" description="Neuferricin homolog">
        <location>
            <begin position="24"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding">
        <location>
            <begin position="61"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="286" mass="32439" checksum="1EB99BF8CB6F3919" modified="2006-04-04" version="1" precursor="true">MFGFVKYLFKLQFLFILAAVLAGLYRTEIKQFANRHAANYVDKADVETVSLQFKAANENTATVLTSAELSKYNGEDGQPIYLALLGSVFDVTRGIKHYGTGCSYNFFVGRDASVAFISGEFEEYDPQTADDVLTLKPNDLLGLANWRDFYEKEYIYKGKLIGRFYDEQGEPTTYYHKYLALLEQAQIAKAEVDELRSKYPGCNIEWSEAKGTRVWCTNTSGDGKERAWTGFPRKLYSRGNKNFNCACVPESELDQIDAEGQAAHGDVMFKTYDNCSSRAKECFYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29P14</accession>
    <accession>A0A6I8ULG2</accession>
    <name>Q29P14_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine-protein kinase meng-po</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">meng</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357410.2">
        <property type="nucleotide sequence ID" value="XM_001357374.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29P14"/>
    <dbReference type="SMR" id="Q29P14"/>
    <dbReference type="FunCoup" id="Q29P14">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29P14"/>
    <dbReference type="GeneID" id="4817987"/>
    <dbReference type="KEGG" id="dpo:4817987"/>
    <dbReference type="eggNOG" id="KOG1345">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_10_3_1"/>
    <dbReference type="InParanoid" id="Q29P14"/>
    <dbReference type="OMA" id="HETACKL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070907">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29P14">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13987">
        <property type="entry name" value="STKc_SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000500">
        <property type="entry name" value="serine/threonine-protein kinase SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MENG-PO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357410.2"/>
        </source>
    </evidence>
    <sequence length="456" mass="52432" checksum="6390A4B733410AC9" modified="2008-10-14" version="2">MGTIEKRSFSFRLRRSFGDGGSTNSRNSNNNSSTCTNHNNQKRCSTPLTPTSTSTGRLEVPGAASVSRRSSIYKKPDKNDGGQIHLIPDVELPLMTFADQYNIEKTLAEGCFAKILLCRHRPTNTLVALKAVHAELTTIKEFQKEFHYNYELSHHHHILSAYAVAFQTMDYYVFAMEHAPYGDLASNIGPNGLHENACKLISEQLSSALGFMHSKNLVHRDLKIENILVFTPDFTRVKLCDFGATTKKGLLVHKVKHTWTSCVPPEQLELIKNERFQCLPISDSWQFGILLYNILTGNPPWQSADWVKDQSYANFMKYEQRKTTKVPDSFRRFSPRLMRCFRKYLSHDPEDRCKITEVTKYMKDRWVECRISTSKSATLISPTHHDQDSCIYLNQREGRLSGDENKLRFKRMMSSYGLDIPIDPVMVRRRVWDWLSTCDASFDPEVESLHGLDMMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZB0</accession>
    <accession>A0A6I8UDN8</accession>
    <name>Q2LZB0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Bifunctional coenzyme A synthase</fullName>
            <ecNumber evidence="20">2.7.1.24</ecNumber>
            <ecNumber evidence="4">2.7.7.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">Ppat-Dpck</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">MV-25-SWS-2005</strain>
            <tissue evidence="24">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:18246"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="18">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the central section; belongs to the eukaryotic CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="20"/>
    <dbReference type="EC" id="2.7.7.3" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001353863.2">
        <property type="nucleotide sequence ID" value="XM_001353827.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZB0"/>
    <dbReference type="SMR" id="Q2LZB0"/>
    <dbReference type="FunCoup" id="Q2LZB0">
        <property type="interactions" value="1599"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZB0"/>
    <dbReference type="GeneID" id="4813423"/>
    <dbReference type="KEGG" id="dpo:4813423"/>
    <dbReference type="eggNOG" id="KOG3220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027827_2_1_1"/>
    <dbReference type="InParanoid" id="Q2LZB0"/>
    <dbReference type="OMA" id="TQCLQSY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070463">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02164">
        <property type="entry name" value="PPAT_CoAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000899">
        <property type="entry name" value="Bifunctional coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000089">
        <property type="entry name" value="Bifunctional coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001985">
        <property type="entry name" value="PRK00777.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="22">
        <location>
            <begin position="146"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00066359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00067394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_001353863.2"/>
        </source>
    </evidence>
    <sequence length="517" mass="57611" checksum="BAE191FC5DEF9EB3" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZS8</accession>
    <accession>A0A6I8VKU6</accession>
    <name>Q2LZS8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphate carrier protein, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Mpcp2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Transport of phosphate groups from the cytosol to the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043037.1">
        <property type="nucleotide sequence ID" value="XM_015187551.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZS8"/>
    <dbReference type="FunCoup" id="Q2LZS8">
        <property type="interactions" value="2033"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZS8"/>
    <dbReference type="GeneID" id="4813787"/>
    <dbReference type="KEGG" id="dpo:4813787"/>
    <dbReference type="eggNOG" id="KOG0767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039456_0_1_1"/>
    <dbReference type="InParanoid" id="Q2LZS8"/>
    <dbReference type="OMA" id="TFREAVP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078579">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZS8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000005">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="61"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="158"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="259"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015043037.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="39227" checksum="D87F2E54FB954432" modified="2008-10-14" version="2">MFSTFYETAKNSPFRSPLGQVQCEGAPAVTSSHELDLEPVHARQISAAAATVDSCEFGSNKYFALCGIGGILSCGTTHTFVVPLDLVKCRLQVDQAKYKNLFHGFKVTVAEEGARGLAKGWFPTLLGYSAQGLCKFGLYELFKVKYANLLGEENSYLYRTSLYLAASASAEFFADIALAPFEAAKVKIQTVPGFANTFREAVPKMLKDEGINAFYKGLVPLWMRQIPYTMMKFACFERTVELLYKYVVPKPRVECSKGEQLVVTFAAGYIAGVFCAIVSHPADVVVSKLNQSKGASAVSVAKSLGFSGMWNGLTPRIIMIGTLTALQWFIYDGVKVALGIPRPPPPEMPASLKAKLEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0C2</accession>
    <accession>A0A6I8UCX3</accession>
    <name>Q2M0C2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL20m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">39S ribosomal protein L20, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mRpL20</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353501.1">
        <property type="nucleotide sequence ID" value="XM_001353465.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0C2"/>
    <dbReference type="SMR" id="Q2M0C2"/>
    <dbReference type="FunCoup" id="Q2M0C2">
        <property type="interactions" value="506"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0C2"/>
    <dbReference type="GeneID" id="4813788"/>
    <dbReference type="KEGG" id="dpo:4813788"/>
    <dbReference type="eggNOG" id="KOG4707">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123265_1_1_1"/>
    <dbReference type="InParanoid" id="Q2M0C2"/>
    <dbReference type="OMA" id="GRRKNVW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070927">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.20:FF:000001">
        <property type="entry name" value="50S ribosomal protein L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.20">
        <property type="entry name" value="Ribosomal protein L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_bL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035566">
        <property type="entry name" value="Ribosomal_protein_bL20_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00062">
        <property type="entry name" value="RIBOSOMALL20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74731">
        <property type="entry name" value="Ribosomal protein L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00076245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001353501.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="17487" checksum="E4FF6A110F436FC5" modified="2006-02-21" version="1">MVFLNIPLLVRARGPDEFWRKRRIFKLAAHYRSRTRNVYSFAIRSVHRALAYATKGRKLKKLDMAQLWTTRVEAGCQQYGVGLETFKEGLTRTDILLNKKMLADLAIWEPRSFEALVKISRERAAVEGFPDIKQRSAFNQVYGLRNLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P2R4</accession>
    <accession>A0A6I8VGX4</accession>
    <name>A0A0R3P2R4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC26532137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041094.1">
        <property type="nucleotide sequence ID" value="XM_015185608.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P2R4"/>
    <dbReference type="GeneID" id="26532137"/>
    <dbReference type="KEGG" id="dpo:26532137"/>
    <dbReference type="InParanoid" id="A0A0R3P2R4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271124">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P2R4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015041094.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18707" checksum="6EB5BA7E5EEF6A3A" modified="2016-01-20" version="1">MVGFHILSIINYQHNMRLFSVLCIVCLIIMTHFVTPEARPIPRSTRATGQEQPQLPLDVGTEDEPKLDFENTMDARGRRMHRFRPQVPPHTPRRKLSEEDGGVSSEEPKLRNEDLRGAIQINCHQPNMIRFKLIEELYCEALSLAEKNPGGTVHYRIHRMPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P725</accession>
    <accession>A0A6I8VMH1</accession>
    <name>A0A0R3P725_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Polycystin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6900263</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polycystin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015044032.1">
        <property type="nucleotide sequence ID" value="XM_015188546.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P725"/>
    <dbReference type="FunCoup" id="A0A0R3P725">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="GeneID" id="6900263"/>
    <dbReference type="KEGG" id="dpo:6900263"/>
    <dbReference type="InParanoid" id="A0A0R3P725"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249995">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003915">
        <property type="entry name" value="PKD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051223">
        <property type="entry name" value="Polycystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046791">
        <property type="entry name" value="Polycystin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF183">
        <property type="entry name" value="AT14535P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20519">
        <property type="entry name" value="Polycystin_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01433">
        <property type="entry name" value="POLYCYSTIN2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="522"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin" evidence="10">
        <location>
            <begin position="128"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="9">
        <location>
            <begin position="332"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="676"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="685"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015044032.1"/>
        </source>
    </evidence>
    <sequence length="705" mass="82005" checksum="78C5CF25214B5394" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P8F0</accession>
    <accession>A0A6I8VL70</accession>
    <name>A0A0R3P8F0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">N-alpha-acetyltransferase 60</fullName>
            <ecNumber evidence="7">2.3.1.259</ecNumber>
            <ecNumber evidence="1">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Naa60</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-methionyl-[transmembrane protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-[transmembrane protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50604"/>
            <dbReference type="Rhea" id="RHEA-COMP:12745"/>
            <dbReference type="Rhea" id="RHEA-COMP:12746"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="ChEBI" id="CHEBI:133414"/>
            <dbReference type="EC" id="2.3.1.259"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the acetyltransferase family. NAA60 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.259" evidence="7"/>
    <dbReference type="EC" id="2.3.1.48" evidence="1"/>
    <dbReference type="RefSeq" id="XP_015043274.1">
        <property type="nucleotide sequence ID" value="XM_015187788.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P8F0"/>
    <dbReference type="SMR" id="A0A0R3P8F0"/>
    <dbReference type="FunCoup" id="A0A0R3P8F0">
        <property type="interactions" value="2122"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P8F0"/>
    <dbReference type="GeneID" id="6900739"/>
    <dbReference type="InParanoid" id="A0A0R3P8F0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244836">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000092">
        <property type="entry name" value="N-alpha-acetyltransferase 60 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045141">
        <property type="entry name" value="NAA60-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14744">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14744:SF15">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015043274.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="32052" checksum="ED9375C60077D6B2" modified="2016-01-20" version="1">MAQFTLYNKHNAPPSNDITRVDCDHVPLCSANDVQLRFLVPDDLTEVRQLCQEWFPIDYPLSWYEDITSSTRFFALAAVYNLAIIGLIVAEIKPYRNVNKEVIANMSDSDELYTRLSGFPMQDKGILPDSMGRTADVGYILSLGVHRSHRRNGIGSLLLDALMNHLTTVERHSVKAIFLHTLTTNQPAIFFYEKRRFTLHSFLPYYYNIRGKGKDGFTYVNYINGGHPPWTLLDHIKHYATKVRHTSTLCVWMASRVQQVVRWFYHKLLMRFNFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAR0</accession>
    <name>A0A6I8UAR0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Ribonuclease Oy</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">RNaseX25</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a guanylyl-uridine-RNA = a 3'-end 2',3'-cyclophospho-GMP-RNA + a 5'-end dephospho-uridine-RNA</text>
            <dbReference type="Rhea" id="RHEA:81323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17356"/>
            <dbReference type="Rhea" id="RHEA-COMP:19658"/>
            <dbReference type="Rhea" id="RHEA-COMP:19659"/>
            <dbReference type="ChEBI" id="CHEBI:173224"/>
            <dbReference type="ChEBI" id="CHEBI:231849"/>
            <dbReference type="ChEBI" id="CHEBI:231850"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an adenylyl-uridine-RNA = a 3'-end 2',3'-cyclophospho-AMP-RNA + a 5'-end dephospho-uridine-RNA</text>
            <dbReference type="Rhea" id="RHEA:81383"/>
            <dbReference type="Rhea" id="RHEA-COMP:17356"/>
            <dbReference type="Rhea" id="RHEA-COMP:19675"/>
            <dbReference type="Rhea" id="RHEA-COMP:19676"/>
            <dbReference type="ChEBI" id="CHEBI:173224"/>
            <dbReference type="ChEBI" id="CHEBI:231879"/>
            <dbReference type="ChEBI" id="CHEBI:231881"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:81384"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the RNase T2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352514.3">
        <property type="nucleotide sequence ID" value="XM_001352478.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAR0"/>
    <dbReference type="FunCoup" id="A0A6I8UAR0">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812142"/>
    <dbReference type="InParanoid" id="A0A6I8UAR0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAR0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000001">
        <property type="entry name" value="Ribonuclease T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026220527" evidence="19">
        <location>
            <begin position="24"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="26"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR633697-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001352514.3"/>
        </source>
    </evidence>
    <sequence length="342" mass="39081" checksum="13089CA712D0F1CD" modified="2020-08-12" version="1">MQSQQIALVIVFLGCLLVAPLSAHDDISDSDESTSGVVERQPQRQTLPDNGSDSDDGDGDDDDDSLYPFDVSGLLDDPTRAMERHQRVHDHNWDVLIFTQQWPVTTCYHWREDNPSQECSLPQKKEFWTIHGIWPTKLGSLGPSFCNKSAEFDVDKLDGISNRLETFWPDLKGATSQEWLWKHEWQKHGTCAMLIEELDDELKYFAQGLSWREQYIMSRILDASDIHPDSNNTVTAINNAIVKALGKNPSIHCLFDTKHEISYLSEIRICFNKSLELIDCDGVKLGDAVSIKYPGGNVTTNCHISSLVHYPSLVPPLQRKREWKFPLVNVYKLLQFLMWFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBU8</accession>
    <name>A0A6I8UBU8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CLIP domain-containing serine protease</fullName>
            <ecNumber evidence="12">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC4812644</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The clip domain consists of 35-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 13">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_001353033.3">
        <property type="nucleotide sequence ID" value="XM_001352997.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBU8"/>
    <dbReference type="FunCoup" id="A0A6I8UBU8">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812644"/>
    <dbReference type="InParanoid" id="A0A6I8UBU8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBU8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035008">
        <property type="term" value="P:positive regulation of melanization defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160032">
        <property type="term" value="P:Toll receptor ligand protein activation cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000015">
        <property type="entry name" value="Atrial natriuretic peptide-converting enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022700">
        <property type="entry name" value="CLIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038565">
        <property type="entry name" value="CLIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF8">
        <property type="entry name" value="ACROSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12032">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00680">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51888">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12 18" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026375302" description="CLIP domain-containing serine protease" evidence="13">
        <location>
            <begin position="27"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Clip" evidence="16">
        <location>
            <begin position="166"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="15">
        <location>
            <begin position="261"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001353033.3"/>
        </source>
    </evidence>
    <sequence length="511" mass="56498" checksum="08025A9B3723F1B4" modified="2020-08-12" version="1">MKSVFGASAVITLLYFLLLGEPSAFAQFNNQRRQVRQNCITPENYYGSCVALRYCPQVVNVFQSTSRERAERYVIALQRSCGTRNFNGDPVLCCTSPQNNPQTTERPSNPFFPKEQPFVGPQPPPDVPNRNPFLTTQRPPTTTTTTTTTRAPVTSAAPLVELRGPVCRGPDTKPGNCVDIKSCPLLLNQLIAKQKDSTFVSFLQASQRICGNVGSTVCCPNGQTATNEPVVTPTVSTEDVPRRLPNVEEGCGYTLNTFKKIVGGEVSRKGAWPWIALLGYDDPSSSPFKCGGTLITARHVLTAAHCIRQDLIFVRLGEHDLSTDTETRHVDINVIRYVSHPEYNRQNGRSDIAILYLERNVQFTDKITPICLPHTPQLRGKSYVGYMPFVAGWGKTQEGGESATVLNELQIPIFDNEQCRESYAKQKRYFSADQFDSAVVCAGVLTGGKDTCQGDSGGPLMIPEPYQNSVRFYLIGVVSYGIGCARPEVPGVYSSTQYFMDWIIERVQETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGK1</accession>
    <name>A0A6I8UGK1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Helix-loop-helix protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HLH4C</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355175.3">
        <property type="nucleotide sequence ID" value="XM_001355139.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGK1"/>
    <dbReference type="KEGG" id="dpo:4814559"/>
    <dbReference type="InParanoid" id="A0A6I8UGK1"/>
    <dbReference type="OMA" id="DMTHMAE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19701">
        <property type="entry name" value="bHLH_TS_HEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000027">
        <property type="entry name" value="Nescient helix-loop-helix 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040238">
        <property type="entry name" value="TAL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864:SF10">
        <property type="entry name" value="HELIX-LOOP-HELIX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864">
        <property type="entry name" value="T-CELL ACUTE LYMPHOCYTIC LEUKEMIA/STEM CELL LEUKEMIA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="109"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355175.3"/>
        </source>
    </evidence>
    <sequence length="168" mass="19140" checksum="B6CB2F342A0952E0" modified="2020-08-12" version="1">MVYDMTHMAEGPPQSISLSRYYMQEDDEMLAPANNGGLLNEDYAASTTLDIEKRFQARMACETAAQPAPPPPPTPAPRRRTTPIAHLDPTELVGLSREERRRRRRATLKYRTAHATRERIRVEAFNVSFAELRKLLPTLPPDKKLSKIEILKLAICYIAYLNHVLETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGM3</accession>
    <name>A0A6I8UGM3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vanin-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Btnd</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. BTD/VNN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355090.3">
        <property type="nucleotide sequence ID" value="XM_001355054.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGM3"/>
    <dbReference type="FunCoup" id="A0A6I8UGM3">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814650"/>
    <dbReference type="InParanoid" id="A0A6I8UGM3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07567">
        <property type="entry name" value="biotinidase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012101">
        <property type="entry name" value="Biotinidase-like_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040154">
        <property type="entry name" value="Biotinidase/VNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043957">
        <property type="entry name" value="Vanin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609:SF14">
        <property type="entry name" value="BIOTINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609">
        <property type="entry name" value="BIOTINIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19018">
        <property type="entry name" value="Vanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026297277" evidence="6">
        <location>
            <begin position="39"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="7">
        <location>
            <begin position="47"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="548"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355090.3"/>
        </source>
    </evidence>
    <sequence length="592" mass="65591" checksum="6D926BF126360399" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGW5</accession>
    <name>A0A6I8UGW5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein Ir7d</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ir7d</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355210.4">
        <property type="nucleotide sequence ID" value="XM_001355174.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGW5"/>
    <dbReference type="FunCoup" id="A0A6I8UGW5">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815133"/>
    <dbReference type="InParanoid" id="A0A6I8UGW5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGW5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056198">
        <property type="entry name" value="LBD_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF30">
        <property type="entry name" value="IONOTROPIC RECEPTOR 40A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24061">
        <property type="entry name" value="LBD_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026197984" evidence="9">
        <location>
            <begin position="21"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ionotropic receptor ligand binding" evidence="10">
        <location>
            <begin position="54"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355210.4"/>
        </source>
    </evidence>
    <sequence length="603" mass="68504" checksum="8B7CA995B4BF043A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH74</accession>
    <name>A0A6I8UH74_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Microtubule-associated protein futsch isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7 8" type="primary">futsch</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355443.3">
        <property type="nucleotide sequence ID" value="XM_001355407.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240742.1">
        <property type="nucleotide sequence ID" value="XM_033384851.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240743.1">
        <property type="nucleotide sequence ID" value="XM_033384852.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240744.1">
        <property type="nucleotide sequence ID" value="XM_033384853.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815526"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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            <begin position="5091"/>
            <end position="5101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="5126"/>
            <end position="5137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="5155"/>
            <end position="5167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="5184"/>
            <end position="5195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="5236"/>
            <end position="5246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="5320"/>
            <end position="5340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="5341"/>
            <end position="5353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="5381"/>
            <end position="5390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="5399"/>
            <end position="5440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="5442"/>
            <end position="5454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="5484"/>
            <end position="5493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="5502"/>
            <end position="5517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="5607"/>
            <end position="5616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="5633"/>
            <end position="5644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="5674"/>
            <end position="5689"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="5748"/>
            <end position="5773"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="5855"/>
            <end position="5865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="5866"/>
            <end position="5875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="5880"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="5904"/>
            <end position="5914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="5950"/>
            <end position="5962"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001355443.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033240742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033240743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033240744.1"/>
        </source>
    </evidence>
    <sequence length="6085" mass="652178" checksum="655B977CC3F7C9E6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHL0</accession>
    <name>A0A6I8UHL0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4815989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355641.4">
        <property type="nucleotide sequence ID" value="XM_001355605.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHL0"/>
    <dbReference type="KEGG" id="dpo:4815989"/>
    <dbReference type="InParanoid" id="A0A6I8UHL0"/>
    <dbReference type="OMA" id="QICRANR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073788">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="45"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355641.4"/>
        </source>
    </evidence>
    <sequence length="155" mass="17663" checksum="6DE5220A3B555A23" modified="2020-08-12" version="1">MSGRDDKADGQMKTNPSELQLHLKELWGPNICGSQLTSVKCWLLHQVGREDTLTSLALKYDTSIGKICRANRMQWQDVLLTRRQVWVPVRLQKNEDIVAELTKRMGSRILNTSVSFANPCTHPPHFHRQSSANPDAFAKDRDPLLITSEHMSIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UI68</accession>
    <name>A0A6I8UI68_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Argininosuccinate lyase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Argl</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355800.3">
        <property type="nucleotide sequence ID" value="XM_001355764.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UI68"/>
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        <property type="interactions" value="730"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816188"/>
    <dbReference type="InParanoid" id="A0A6I8UI68"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="argininosuccinate lyase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="3">
        <location>
            <begin position="370"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001355800.3"/>
        </source>
    </evidence>
    <sequence length="468" mass="52571" checksum="B5EB87178636254E" modified="2020-08-12" version="1">MATSKNSYQLWGGRFSEKPSEALQALNISLPYDSRLYADDLDASKAYAEALQRAGYLNTAEADKLVKSLELIRYDWIERTIKFLPTDEDVHTVNERLLVEITGELGQRLHTGRSRNDQVVTDMKLWLRRGIRETLGRLSRVIESAVKQAEVHLGVLMPGYTHMQRAQPVQFSHWLLSHAFALREDCLRLVELRDRANVLPLGSGALAGNPLGIDRLWLAERLGFSGVTANSMHAVGDRDFVVDFIYCCSLVSLHLSRFAEDLIIYSTKEFDFIRLHDSLSSGSSLMPQKRNPDSLELIRGISGVISSNLTGIMMTIKGTPSTYNKDLQFDKEYCFQSFDKLKQVLDVTQGVIQTMQVQRDTMEAALSPDMLATDWAYYLVKKGIPFRQAHHFIGRVVSLAEQRGVGITEVPLGELQKICPAFGSDIASVADYANNVQQYDVIGGTATASIEEQLRLLNNFVKDMRKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJJ8</accession>
    <name>A0A6I8UJJ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UDP-glucuronosyltransferase 1-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Ugt37C2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356533.3">
        <property type="nucleotide sequence ID" value="XM_001356497.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJJ8"/>
    <dbReference type="FunCoup" id="A0A6I8UJJ8">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817450"/>
    <dbReference type="InParanoid" id="A0A6I8UJJ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJJ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
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            <begin position="1"/>
            <end position="20"/>
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            <begin position="21"/>
            <end position="528"/>
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        <location>
            <begin position="485"/>
            <end position="504"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001356533.3"/>
        </source>
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    <sequence length="528" mass="59299" checksum="C5F81835E94C2A4A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKN7</accession>
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            <fullName evidence="3">Protein phosphatase 1F</fullName>
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        <name evidence="3" type="primary">LOC4817233</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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    </dbReference>
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        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
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        <property type="entry name" value="PPM-type phosphatase domain"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="167"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356903.2"/>
        </source>
    </evidence>
    <sequence length="436" mass="49055" checksum="8AEF9F09D093F912" modified="2020-08-12" version="1">MSADSIKEPAAPAVECAHMLEFKEFLAATAAKASATTEDLTRNCVTRTCNETYKVSVEERHAELVAAIWQQLNSRGCPAVFRLKLLHQATQLLEKEICYAKECEVSVEGPTQYDLLKLQKFVSNQMDKLLIKLTDNSEMERLKDYADEGVICQSPEHCMCGLAQTALHTSAAVKNKPRKMEDRHVCLDRFGAIYELRNRDCRFFGVFDGHSGSLSASYVRSQLPQLLADQLRQADQQTETQSSDFYRNAFEAAFLQADERFIQKRITSGTTCVCALINRDQLYIAWVGDSKALLVGKRTQLQLVKPHKPESPDERKRIEAAGGTVLHAQGQWRVNGILNVARSIGDYCLETVIAEPDFVDVHLNEAHDFLVLGTDGLWDHVPETFIIDTVYECLADPTTKLDDIPKLLIEAAKERDSQDNITAVIVLLKPRHQIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKT2</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein dbr</fullName>
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        <name evidence="5" type="primary">dbr</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">MV-25-SWS-2005</strain>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Znf_AD"/>
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        <property type="entry name" value="BCDNA.LD26519-RELATED"/>
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    </dbReference>
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    </dbReference>
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            <begin position="7"/>
            <end position="85"/>
        </location>
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            <begin position="258"/>
            <end position="305"/>
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    </feature>
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        <location>
            <begin position="735"/>
            <end position="805"/>
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            <begin position="835"/>
            <end position="1030"/>
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            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
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        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
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        <location>
            <begin position="751"/>
            <end position="761"/>
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        <location>
            <begin position="787"/>
            <end position="798"/>
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        <location>
            <begin position="883"/>
            <end position="893"/>
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        <location>
            <begin position="972"/>
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        <location>
            <begin position="984"/>
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        <location>
            <begin position="1011"/>
            <end position="1030"/>
        </location>
    </feature>
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        <location>
            <position position="9"/>
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        <ligand>
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        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357097.2"/>
        </source>
    </evidence>
    <sequence length="1030" mass="112871" checksum="72D46C05D695B31E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMC2</accession>
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        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S3</fullName>
        </recommendedName>
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        <name evidence="11" type="primary">RpS3</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Sophophora</taxon>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">MV2-25</strain>
        </source>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
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        <text evidence="1 7">Belongs to the universal ribosomal protein uS3 family.</text>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK04191.1"/>
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    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="9">
        <location>
            <begin position="23"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="201"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357699.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="27472" checksum="3AA04A4F05162CEA" modified="2020-08-12" version="1">MNASLPISKKRKFVSDGIFKAELNEFLTRELAEDGYSGVEVRVTPSRTEIIIMATKTQQVLGEKGRRIRELTAMVQKRFNFETGRIELYAEKVATRGLCAIAQAESLRYKLTGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPCNDYVETATRHVLLRQGVLGIKVKIMLPYDPKNKIGPKKPLPDNVSVVEPKEEKIYETPETEYKIPPPSKPLDDLSEAKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMM8</accession>
    <name>A0A6I8UMM8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Translin-associated protein X</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Trax</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the translin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357923.2">
        <property type="nucleotide sequence ID" value="XM_001357886.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMM8"/>
    <dbReference type="FunCoup" id="A0A6I8UMM8">
        <property type="interactions" value="2311"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800697"/>
    <dbReference type="InParanoid" id="A0A6I8UMM8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.190:FF:000007">
        <property type="entry name" value="FI16517p1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.200:FF:000001">
        <property type="entry name" value="Translin-associated factor X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.190">
        <property type="entry name" value="Translin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.200">
        <property type="entry name" value="Translin, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016069">
        <property type="entry name" value="Translin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016068">
        <property type="entry name" value="Translin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357923.2"/>
        </source>
    </evidence>
    <sequence length="299" mass="34452" checksum="B8A7D653BD957FA8" modified="2020-08-12" version="1">MSKHAGGNHRGNAHKKRLPQVAVDEDNPIVQAFRNYSIELTTKHDRHERIVKLSRDITIESKRIIFTLHSIDSRKQNKEKILEEAQKRLQKLIEVNFRAIALELRDQDVYQFRNAYSAGLQEFIEAYTYMEYLSRDDNDETPKSVSDWQALQSVMQYVEDPSKAKDEGASVEEMDVDEEKGGEEGQAKKFQFFIDPTEYVLGLSDLTGELMRRCINSLGSGDTDTCMETCSTLQMFYTGYISLNLQRARELWRKITTMRQSVLKAENVCYNVKVRGGEAAIWGSNFDQKPAEDVDEGFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMY6</accession>
    <name>A0A6I8UMY6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Endothelin-converting enzyme homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Nepl14</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358114.3">
        <property type="nucleotide sequence ID" value="XM_001358077.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMY6"/>
    <dbReference type="FunCoup" id="A0A6I8UMY6">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800926"/>
    <dbReference type="InParanoid" id="A0A6I8UMY6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF240">
        <property type="entry name" value="GH14155P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026058345" evidence="9">
        <location>
            <begin position="20"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="11">
        <location>
            <begin position="61"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="10">
        <location>
            <begin position="485"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001358114.3"/>
        </source>
    </evidence>
    <sequence length="693" mass="79095" checksum="C1D38F17C91D9035" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNC0</accession>
    <name>A0A6I8UNC0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lateral signaling target protein 2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4801147</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negative regulator of epidermal growth factor receptor (EGFR) signaling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lst-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358285.4">
        <property type="nucleotide sequence ID" value="XM_001358248.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNC0"/>
    <dbReference type="FunCoup" id="A0A6I8UNC0">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801147"/>
    <dbReference type="InParanoid" id="A0A6I8UNC0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061952">
        <property type="term" value="P:midbody abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15731">
        <property type="entry name" value="FYVE_LST2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000073">
        <property type="entry name" value="myotubularin-related protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043269">
        <property type="entry name" value="FYVE_LST2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051118">
        <property type="entry name" value="LST-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46465">
        <property type="entry name" value="LATERAL SIGNALING TARGET PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46465:SF2">
        <property type="entry name" value="LATERAL SIGNALING TARGET PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="9">
        <location>
            <begin position="916"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="495"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="775"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="360"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="426"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="542"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="560"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="573"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="628"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="778"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="821"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358285.4"/>
        </source>
    </evidence>
    <sequence length="1008" mass="112089" checksum="64EE266D8D59C87E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP70</accession>
    <name>A0A6I8UP70_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MED6</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358588.3">
        <property type="nucleotide sequence ID" value="XM_001358551.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP70"/>
    <dbReference type="FunCoup" id="A0A6I8UP70">
        <property type="interactions" value="2753"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801509"/>
    <dbReference type="InParanoid" id="A0A6I8UP70"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.580:FF:000005">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016820">
        <property type="entry name" value="Mediator_Med6_met/pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023869">
        <property type="entry name" value="Mediator_MED6_meta/pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR023869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358588.3"/>
        </source>
    </evidence>
    <sequence length="258" mass="29133" checksum="7DB43E245462E3B1" modified="2020-08-12" version="1">MANRQMAQANANAPENHLRLSWHDTQMMATLSPQTVMDYFCRKSNPFYDHLCNNETIRMQRLGPEHLHNMIGLEYILLHVAEPILYVIRKQHRHNPSEATPIADYYIIGGTVYKAPDLANVINARILNTVVNLQSAFEEASSYARYHPNKGYTWDFSSNKVLSDKSKSDKKDANAVKDENSGTLFQKQRVDMLLAELLRKFPPPIPPMLQNLQQQPMAAGDEMTPGGINATDMNNATGPLDIKTEGVDMKPPPEKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPJ6</accession>
    <name>A0A6I8UPJ6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL1m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mRpL1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358838.2">
        <property type="nucleotide sequence ID" value="XM_001358801.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPJ6"/>
    <dbReference type="FunCoup" id="A0A6I8UPJ6">
        <property type="interactions" value="1047"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801804"/>
    <dbReference type="InParanoid" id="A0A6I8UPJ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000008">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005879">
        <property type="entry name" value="Ribosomal_uL1_mit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01170">
        <property type="entry name" value="rplA_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001358838.2"/>
        </source>
    </evidence>
    <sequence length="354" mass="40228" checksum="EA78DD95A031BB64" modified="2020-08-12" version="1">MQSILSSMRALAFRQPTGDAIRRLHLTAISEAARKGTREKARKKKVKVEVKKVGFIPHNQRDKKVNVKRADKHVDDSWKQVSKDDVYVGRYYRWPVYSVAEAIQCHRETHHPSMYNVPNAPLNVEIELNMQGEKATRFVDNFQRMAMIPHKFEHGEERKIIVFTKGNDEILEAREAGASLVGGVELIKDITGGELLLSDYQFIIAHPNILPELVALRGLMKRKFPNPKSETLGANLAEMIVKFSSGISYSATKDEYQQNFGLISASVGPLDMDAQKLEENLKHLLQDINTMRPKREGKFITRVLLKSPPSSEQLKIDPFVYVPEMWDKSSAKVKREEVKKQEDPAEPQEAVAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPW9</accession>
    <name>A0A6I8UPW9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 17">Vesicle-fusing ATPase</fullName>
            <ecNumber evidence="4 17">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC4801877</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 17">Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="4 17"/>
    <dbReference type="RefSeq" id="XP_001358901.4">
        <property type="nucleotide sequence ID" value="XM_001358864.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPW9"/>
    <dbReference type="FunCoup" id="A0A6I8UPW9">
        <property type="interactions" value="2147"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801877"/>
    <dbReference type="InParanoid" id="A0A6I8UPW9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19504">
        <property type="entry name" value="RecA-like_NSF-SEC18_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000032">
        <property type="entry name" value="vesicle-fusing ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000010">
        <property type="entry name" value="vesicle-fusing ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000026">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000031">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000166">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000187">
        <property type="entry name" value="Vesicular-fusion ATPase SEC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054419">
        <property type="entry name" value="NSF_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039812">
        <property type="entry name" value="Vesicle-fus_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078:SF3">
        <property type="entry name" value="VESICLE-FUSING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078">
        <property type="entry name" value="VESICULAR-FUSION PROTEIN NSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21964">
        <property type="entry name" value="NSF_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 17" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="19">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="252"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="535"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU367045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001358901.4"/>
        </source>
    </evidence>
    <sequence length="746" mass="82966" checksum="C5E577AAF75E8F7A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ28</accession>
    <name>A0A6I8UQ28_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">G-patch domain and KOW motifs-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4802068</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359061.1">
        <property type="nucleotide sequence ID" value="XM_001359024.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ28"/>
    <dbReference type="FunCoup" id="A0A6I8UQ28">
        <property type="interactions" value="1733"/>
    </dbReference>
    <dbReference type="GeneID" id="4802068"/>
    <dbReference type="KEGG" id="dpo:4802068"/>
    <dbReference type="InParanoid" id="A0A6I8UQ28"/>
    <dbReference type="OMA" id="CINTAKY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070302">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13152">
        <property type="entry name" value="KOW_GPKOW_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041993">
        <property type="entry name" value="GPKOW_KOW1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045166">
        <property type="entry name" value="Spp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026822">
        <property type="entry name" value="Spp2/MOS2_G-patch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818">
        <property type="entry name" value="G PATCH AND KOW-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818:SF2">
        <property type="entry name" value="G-PATCH DOMAIN AND KOW MOTIFS-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12656">
        <property type="entry name" value="G-patch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spp2/MOS2 G-patch" evidence="4">
        <location>
            <begin position="149"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="302"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="302"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="398"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="433"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="493"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359061.1"/>
        </source>
    </evidence>
    <sequence length="519" mass="58763" checksum="96CD32BD0268A379" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQA1</accession>
    <name>A0A6I8UQA1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Enhancer of split mbeta protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">E(spl)mbeta-HLH</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359178.1">
        <property type="nucleotide sequence ID" value="XM_001359141.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQA1"/>
    <dbReference type="SMR" id="A0A6I8UQA1"/>
    <dbReference type="FunCoup" id="A0A6I8UQA1">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="GeneID" id="4802210"/>
    <dbReference type="KEGG" id="dpo:4802210"/>
    <dbReference type="InParanoid" id="A0A6I8UQA1"/>
    <dbReference type="OMA" id="NSTGPMW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073111">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19741">
        <property type="entry name" value="bHLH-O_ESMB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000009">
        <property type="entry name" value="Transcription factor HES-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="9">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359178.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="21874" checksum="1C3CEAE1249A32F7" modified="2020-08-12" version="1">MVLEMEMSKTYQYRKVMKPMLERKRRARINKCLDELKDIMVECLTQEGEHITRLEKADILELTVEHMKKLRAQKQLRLSSSVSPSASSADSKLSIAESFRAGYVHAANEVSKTLAAVPGVTVDLGTQLMSHLGHRLNYLQVVVPTLPIGMGVPLQTPVSGVDDAALVTPPPSECGSLESGTCSPAPSEASSTSGPMWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT21</accession>
    <name>A0A6I8UT21_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4803815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360472.4">
        <property type="nucleotide sequence ID" value="XM_001360435.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT21"/>
    <dbReference type="FunCoup" id="A0A6I8UT21">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803815"/>
    <dbReference type="InParanoid" id="A0A6I8UT21"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001360472.4"/>
        </source>
    </evidence>
    <sequence length="106" mass="12336" checksum="B22624858F190BFB" modified="2020-08-12" version="1">MDKIEDFPNETELRVHNAMAEAPAITGPTDNDGNIDEPIIYDYMRYPNKKKRNKLAAKTFNTNRTVHFRKFVELVTYTTNGKINIQEVALRTEEEQSKSTMKRDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTH0</accession>
    <name>A0A6I8UTH0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">POSH</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SH3RF family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001360631.2">
        <property type="nucleotide sequence ID" value="XM_001360594.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTH0"/>
    <dbReference type="FunCoup" id="A0A6I8UTH0">
        <property type="interactions" value="662"/>
    </dbReference>
    <dbReference type="GeneID" id="4804000"/>
    <dbReference type="KEGG" id="dpo:4804000"/>
    <dbReference type="InParanoid" id="A0A6I8UTH0"/>
    <dbReference type="OMA" id="HKQRRFL"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078519">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16750">
        <property type="entry name" value="RING-HC_SH3RF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11787">
        <property type="entry name" value="SH3_SH3RF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11785">
        <property type="entry name" value="SH3_SH3RF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000077">
        <property type="entry name" value="E3 ubiquitin-protein ligase SH3RF1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035816">
        <property type="entry name" value="SH3RF1/SH3RF3_SH3_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028502">
        <property type="entry name" value="SH3RF3_RING-HC_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF51">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="12"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="143"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="203"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="434"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="798"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="81"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="280"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="572"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="638"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="720"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="644"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001360631.2"/>
        </source>
    </evidence>
    <sequence length="858" mass="94214" checksum="0BF677FA1F81313C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU89</accession>
    <name>A0A6I8UU89_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbonic anhydrase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CAH14</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360973.2">
        <property type="nucleotide sequence ID" value="XM_001360936.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU89"/>
    <dbReference type="FunCoup" id="A0A6I8UU89">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="GeneID" id="4804403"/>
    <dbReference type="KEGG" id="dpo:4804403"/>
    <dbReference type="InParanoid" id="A0A6I8UU89"/>
    <dbReference type="OMA" id="YAMEMQV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073622">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF233">
        <property type="entry name" value="CARBONIC ANHYDRASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="2">
        <location>
            <begin position="55"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360973.2"/>
        </source>
    </evidence>
    <sequence length="295" mass="33446" checksum="A2DE637A48C2FDD9" modified="2020-08-12" version="1">MLSPIIDFWLQFMQGFNLVMEYVQHSAILKILVSCIMSLAFLNIVRAGGLMIRKVAMAIAPKKQQRKLAPQPTPINIVRSMARKMELGSLLDWKHYDDMPLAMLLENNGSTVILRICCEPYLVPQLSGGDLPGEYHFVEACFKWGPLRAEHSIDSMKFSLEMQVLHRCNQDHVPFEYVTVSYLFVIMGNGNGPLTTITENLRCIARPDSQMELPAFDLASLMKPFDSNFYSYEGTYDNGAQVLPTKWLICTYIFAISSQQVSQFSTLCGRDGTSIHCNARKEQPLGNRSVNFHIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUB7</accession>
    <name>A0A6I8UUB7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LisH domain-containing protein C1711.05</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4804326</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360903.4">
        <property type="nucleotide sequence ID" value="XM_001360866.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUB7"/>
    <dbReference type="FunCoup" id="A0A6I8UUB7">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804326"/>
    <dbReference type="InParanoid" id="A0A6I8UUB7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032061">
        <property type="entry name" value="DUF4802"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16060">
        <property type="entry name" value="DUF4802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4802" evidence="2">
        <location>
            <begin position="401"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360903.4"/>
        </source>
    </evidence>
    <sequence length="470" mass="51384" checksum="E556532D92FA435A" modified="2020-08-12" version="1">MAPFKLKFRMGSSRSTSQEHDPDPQVNEALLGNQQQQQQQHQQQQELLQQHVVEASSSSSLDLADCNGLGPMSSERKLLLPQNKALNLNSNSMRYLNQSRTPCINESLTDTDADILPTTPPPSYEHVLEEDTRLSQHQRQVSLDSAATPNQNSRRSSANSSRHSAAQLSAALEEQLEGGDSSSSTSSSLCNDPDCQQNQQNLNQNYSHCGGSNISNSNNNNNNEGEPIAEGVFELEQQSDTEFDCPEDDLNTISVNEFLLETEMAASSRCTEKLYAGEEDGDDADGQCTDDQCAQCSEERGAQGEEGALDGDEASTSSQAAAFYDPLLNRGSYNYNEGGNSNSRPVSSNSQQCQEACCVGSASGSVASRSRSGRRQQQLQQQHQQPNCHTNLLTPEMMSNKTSKEIYKDLAKQWGITCKMSESCRCMDCQSNYFDCDYDDNEHHKTDGGLGAGTPMFISEVMHGSGCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUM0</accession>
    <name>A0A6I8UUM0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CG18067 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361167.3">
        <property type="nucleotide sequence ID" value="XM_001361130.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUM0"/>
    <dbReference type="FunCoup" id="A0A6I8UUM0">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804637"/>
    <dbReference type="InParanoid" id="A0A6I8UUM0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026330697" description="CG18067 protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361167.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="27253" checksum="16D474EFD9E3276B" modified="2020-08-12" version="1">MGLGRTLIILGLIGSVVACLASAMPQGPGSDDQKILRSQGSTFLSNGAGQIIRGADGKTILIGSDGRRIIADTSISEEDASQDDDSFLVQNNGGSSISSNDNNIFINGGQSNIITSNGKTLQYGTGNINYSGYNGHSINVVNGVITLREGGKQYTFEAQPPNVEKREAIVINGQPATVQYSHGDVIVELADQTVIAIVGNTSFIGDRTSFENRDKLQAEAKTYAENIQRQVQEGLKKSLQQMNEDLQRSLGNIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUR1</accession>
    <name>A0A6I8UUR1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Larval cuticle protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Lcp9</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361218.2">
        <property type="nucleotide sequence ID" value="XM_001361181.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUR1"/>
    <dbReference type="FunCoup" id="A0A6I8UUR1">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804699"/>
    <dbReference type="InParanoid" id="A0A6I8UUR1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF228">
        <property type="entry name" value="CUTICULAR PROTEIN 11A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026134010" evidence="3">
        <location>
            <begin position="17"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361218.2"/>
        </source>
    </evidence>
    <sequence length="92" mass="10267" checksum="2562AEE9647D9019" modified="2020-08-12" version="1">MKFVIVLACLLALAYANEEADVLKSDSEVNVEDFKYAYELSNHIKAVQSGVLKEHDNWVVSGEYEYVAPNGQLVKVVYTADETGYHPKVVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV66</accession>
    <name>A0A6I8UV66_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GstE2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361307.2">
        <property type="nucleotide sequence ID" value="XM_001361270.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV66"/>
    <dbReference type="FunCoup" id="A0A6I8UV66">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="GeneID" id="4804806"/>
    <dbReference type="KEGG" id="dpo:4804806"/>
    <dbReference type="InParanoid" id="A0A6I8UV66"/>
    <dbReference type="OMA" id="QEANNEG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074568">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF4">
        <property type="entry name" value="FI01423P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="89"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361307.2"/>
        </source>
    </evidence>
    <sequence length="219" mass="24395" checksum="43F77A92FB0643C3" modified="2020-08-12" version="1">MSDIVLYGMDISPPVRACLLTLRALGLAFEYKEVNLFAGEHRSPEFLQKNPQHTVPLLDDGGALIWDSHAIVCYLVDKYAKTDELYPKDLVKRAQVNQRLYFDASILFMALRNISVPYFLHNVSLVPKEKVNNVSEAYGHLETFLGESPYVTGDTLTVADLCCAATAASLAAVLDLDTVKYPKIAAWLERISKLPHYTEDNLRGANKYIGMLKGSLTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVC5</accession>
    <name>A0A6I8UVC5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GalT1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001361380.2">
        <property type="nucleotide sequence ID" value="XM_001361343.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVC5"/>
    <dbReference type="FunCoup" id="A0A6I8UVC5">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804892"/>
    <dbReference type="InParanoid" id="A0A6I8UVC5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000054">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF314">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361380.2"/>
        </source>
    </evidence>
    <sequence length="462" mass="52769" checksum="DE89094FF9153C37" modified="2020-08-12" version="1">MNNAGTGDEEQCALLADSSVSSGSTASVSDEETAARQPRIKLRGTMKLQRRTRLPLPRILRRFGCYTLMALFIFLLLLVYLPLMYMDGHKRRVGLPDWTLETSRSIGDYIDPVQETALIVPREFCRNKTFLIIAVCTSLGNFNQRQTIRETWGNTSEFNYGAFVKLHGHLKGNYLPPMTARLKHYAEYLSGEGDSLSASVRIVFIVGRSKYESLVGNETLSRVHSEAEQYNDIIQESFVDSYNNLTLKSVMALKHISRSCANSTAYFLKCDDDTFVNVPNLLHFLFGGSVPLYNDTLDYHDRYTFLVKSPQNRLNDTSGVMYGHQFCNVVPVNDVSSKWYMPSYMYPPESYPKYLSGAGYLLSIDVVQRLYEASLNTTLVYLEDVYITGLCAQRAHITRHHHPLFSFAHSKHLCAFKGSITQHQVKDDTMVEAWNYVSDYKIRCPPPGRYFNHMRLHKRPNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW47</accession>
    <name>A0A6I8UW47_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc transporter ZIP11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Solute carrier family 39 member 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Zrt- and Irt-like protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Zip48C</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361823.1">
        <property type="nucleotide sequence ID" value="XM_001361786.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW47"/>
    <dbReference type="FunCoup" id="A0A6I8UW47">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="GeneID" id="4805421"/>
    <dbReference type="KEGG" id="dpo:4805421"/>
    <dbReference type="InParanoid" id="A0A6I8UW47"/>
    <dbReference type="OMA" id="MIFVVIE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072152">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF211">
        <property type="entry name" value="ZINC TRANSPORTER ZIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="273"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001361823.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="36088" checksum="2F6EEF6A8299542E" modified="2020-08-12" version="1">MIPGYGPVTQALLGTLLTWGLTAAGAALVIFVRGTQRRSLDAALGFAAGVMIAASFWSLLKPAIEMAESSHLYGVYSFVPVAGGFLLGSFFVYGCDKLMSYLGLNSTKMMIQMTQSSKDKADIAIEDLKRNGNGERLASKSLESFSDCLSVQHSGESRRRKKGSINEMEQCTYTTTEEEKRAQEALSQWKRIMLLVVAITVHNIPEGLAVGVSFGAIGTSNSSTFESARNLAIGIGIQNFPEGLAVSLPLHAAGFSVGRALWYGQLSGMVEPIFGVLGAVAVTFANLILPYALSFAAGAMIYIVSDDILPEAHASGNGTIATWGTVSGFLIMMCLEVALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWJ9</accession>
    <name>A0A6I8UWJ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4805665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001362026.1">
        <property type="nucleotide sequence ID" value="XM_001361989.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWJ9"/>
    <dbReference type="FunCoup" id="A0A6I8UWJ9">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="GeneID" id="4805665"/>
    <dbReference type="KEGG" id="dpo:4805665"/>
    <dbReference type="InParanoid" id="A0A6I8UWJ9"/>
    <dbReference type="OMA" id="RIRCSMM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081012">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019080">
        <property type="entry name" value="YqaJ_viral_recombinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39953">
        <property type="entry name" value="RE54151P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39953:SF1">
        <property type="entry name" value="RE54151P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09588">
        <property type="entry name" value="YqaJ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YqaJ viral recombinase" evidence="1">
        <location>
            <begin position="109"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="YqaJ viral recombinase" evidence="1">
        <location>
            <begin position="328"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001362026.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="52087" checksum="8915377752EA3E2E" modified="2020-08-12" version="1">MKGMIIVPMDIDPGFRKATIHNLPTLNSGMVYNFCLAASSEKSQNISTDKNDLLADYVQLRQKGSICELKAMVFSASEFADTDTHANVAIKVLENPRAIAEGQCSRCPDSEVCPHIVAFTFWLLHKSTERQPSAVVEFWGHELELLVQTEPTRAIQICDMIPKDEVRLQSDASSQKMNDSDGQAFLSTVLEEIAICGRGSALYRQCVETSDEFETLFVHHVLLRANEYNIHDVQGFAQHMEQQAQIGLIESLNDVSRNQYKSRLWVETQYMRIRCSMINLLATRKTAEDDEQIFNMMFCKGRDTNTDARMQLKQHKRFILKQTEKLENKEYVECGLMLHESYPFLCATPDGITDDHIVEIKAPKSEEDFQRYLEAGESIAPKYMAQIQIQMFVANVSKALYCVLSPIFETNGALHYVWVEADPEFVASLITMADDFWRDIVFPRLLNTYPQITYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXW3</accession>
    <name>A0A6I8UXW3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pseudouridine-5'-phosphatase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132888.2">
        <property type="nucleotide sequence ID" value="XM_002132852.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXW3"/>
    <dbReference type="KEGG" id="dpo:6903189"/>
    <dbReference type="InParanoid" id="A0A6I8UXW3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246755">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXW3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132888.2"/>
        </source>
    </evidence>
    <sequence length="387" mass="43346" checksum="C20EB7BA4E98A997" modified="2020-08-12" version="1">MCNRCCQPVGCAPSCCEPNICYCIFDLESSVFDTRNVYRRALIELAASYDRQVPEVLLLQVGVMATGEMAELIVRKCRIPVSWEHFLLQVNERASELIGNPPLMEGVERLVRHLKKCCIGMALISSSCEALYCQKIRGREAFFQHFETLVCADDPQLKQPKPEPDVYLIAMSRLGEADASSTLVFDGTIKGVQAASDARLKVVMVPEKGLPQCWSERAAVRLETLAQFRPEWFGIAPLSREAPPPRPKSKPRARSRLSQAQQESSEERETEGEGSEEGKEGEPGKKDEGPEKREEQPQKKQPEPERKEAEPERKEAEPERKVEAKPEPRDDNDNGPGADDAGGPNKAGGGGRNWDRVRNSVRRSLKPLRRKSSLRTETTEEKPPAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXZ1</accession>
    <name>A0A6I8UXZ1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein F58A4.6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6902447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132992.2">
        <property type="nucleotide sequence ID" value="XM_002132956.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXZ1"/>
    <dbReference type="FunCoup" id="A0A6I8UXZ1">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902447"/>
    <dbReference type="InParanoid" id="A0A6I8UXZ1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032072">
        <property type="entry name" value="DUF4807"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36693">
        <property type="entry name" value="GH02722P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36693:SF1">
        <property type="entry name" value="GH02722P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16065">
        <property type="entry name" value="DUF4807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002132992.2"/>
        </source>
    </evidence>
    <sequence length="218" mass="25629" checksum="1C935887159C023D" modified="2020-08-12" version="1">MSGPLTVCVCDYWKRRHYYNCPPLDQEEAAPAGELRRLRLAMNAINGHYCLREFVQQRALALRLQSHHGVELIWLELEPPARETIDYKWAEILAHTIWQHIAVEHLMSWLSTLGGGFSALGEQFERCAKTAGRISLQQLKIGLRLGDPCLQARCKLYYGISLIQRGHLRAAKHLIRDQFKFALVHREKDRRLVRMCQGIWMRLVYEYQQRRQRIKQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZM9</accession>
    <name>A0A6I8UZM9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6901551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134226.2">
        <property type="nucleotide sequence ID" value="XM_002134190.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZM9"/>
    <dbReference type="KEGG" id="dpo:6901551"/>
    <dbReference type="InParanoid" id="A0A6I8UZM9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134226.2"/>
        </source>
    </evidence>
    <sequence length="148" mass="16806" checksum="59639D6AB1C6CFD6" modified="2020-08-12" version="1">MYFRKRLPKKHGPRLCIGKSSLDGLAFKLDIFKQRHGIGIKAVLARKKSLKPFRRHRLVPTVRATGERVQRESAVEEESTLEELGKEPNRKPGFFELLLIVGLLILSIFSLSQVAMSGYTFISKKYLILKVLGLSHLANVSSNNIYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V016</accession>
    <name>A0A6I8V016_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial carrier homolog 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6901822</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134490.2">
        <property type="nucleotide sequence ID" value="XM_002134454.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V016"/>
    <dbReference type="KEGG" id="dpo:6901822"/>
    <dbReference type="InParanoid" id="A0A6I8V016"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V016">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780:SF18">
        <property type="entry name" value="LD43650P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780">
        <property type="entry name" value="MITOCHONDRIAL CARRIER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="209"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002134490.2"/>
        </source>
    </evidence>
    <sequence length="389" mass="42968" checksum="30AE0B89F920F7C6" modified="2020-08-12" version="1">MSGNSPGFGIEGDDYFVLPDSEDDLALAVIPLRNNGAGDGNYVEDGLHLRDPEQGPHRLVAGRSDINEIRDMVALNGQDHIESEEGPLQNNNRNNEQDGLNDPLEFFCTGVLWTTLMHPFHHAGVLMQLGFDPFPGEIVRVGLLGSRSLLPDTQHLIGYIHQSDGFFALFRGLKASIAGNFLSYLMESAMVAMGLGTRKLTHKTGTSVKDVSRNLLVRSIRLTLNLVVTQPLTVVSTRQIAQFIGCENVYKTLAQTICKIFTEEGVAGFYAGFVPRLLRNIGVMAVTTVATALFFHYWPQHSNIQVLNEVIVNVSSSYALHPLSVVGTCMDVQGSPLAAAMPSQMPHYEHWTKCFYDIYQGNGFYHRGGVLFWRTVPVKGLNDFMGRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0J4</accession>
    <name>A0A6I8V0J4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA methyltransferase 5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6900514</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in mitochondrial tRNA methylation. Specifically methylates the N1 position of guanosine-37 in various tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="11">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="11"/>
    <dbReference type="RefSeq" id="XP_002134903.3">
        <property type="nucleotide sequence ID" value="XM_002134867.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0J4"/>
    <dbReference type="FunCoup" id="A0A6I8V0J4">
        <property type="interactions" value="1166"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900514"/>
    <dbReference type="InParanoid" id="A0A6I8V0J4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000102">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="12">
        <location>
            <begin position="153"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="280"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="308"/>
            <end position="309"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002134903.3"/>
        </source>
    </evidence>
    <sequence length="465" mass="53687" checksum="95A3551E82534405" modified="2020-08-12" version="1">MIFVPRLEVPKITIISTRLRHYFRNMNCSELLPPASVRGMRELQRAEFQKRVQVPQLRVPEQQVQRVIPLVKKFLLKMEHMHPVRAVDKSREILLHPMPIKAWESLPTEDLQKQQVTQENFSFVELELNYENWSANEILKSVLPEEEEGMTSYSRIGHIVHLNLRDHLLPYKQLIGEVLRDKLPNCRTVVNKASSIDNTYRNFQLELICGEAEYQVETKENGIPFEFDFSKVYWNPRLSTEHERIVKLLQPGDVLYDVFAGVGPFSVPAAKKRCHVLANDLNPVSFHWLQHNAKRNKCLSNIKMSNKDGREFILKELRADLLQRLRLTDTSSYATHITMNLPAMAVEFLDAFRGLYTDNELADISDAVVFPTVHVYSFAKGENTKALVRAQVEQNLASTLDEKQLQGISFVRNVAPNKDMYRVSFKLTRHLLTTPKEAEANNVRKRCAEDEKVDPEVAATKVKCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1F6</accession>
    <name>A0A6I8V1F6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein Obp73a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Obp73a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135498.3">
        <property type="nucleotide sequence ID" value="XM_002135462.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1F6"/>
    <dbReference type="FunCoup" id="A0A6I8V1F6">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900159"/>
    <dbReference type="InParanoid" id="A0A6I8V1F6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052295">
        <property type="entry name" value="Odorant-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066">
        <property type="entry name" value="ODORANT-BINDING PROTEIN 59A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066:SF18">
        <property type="entry name" value="ODORANT-BINDING PROTEIN 73A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002135498.3"/>
        </source>
    </evidence>
    <sequence length="277" mass="31327" checksum="65A60B87F048A5E1" modified="2020-08-12" version="1">MKITQLLYILCVVVITSIGVIDAVGYIIRFETKKSRCLNPPRTARKVESVITHCQDEVKTKLVNGGCRNQGILSHPNGLIASTEAYEILKEQVSQDQPPIDPNDDTIDYMWPSVPSGSSGVELDHPPTGPLNASHYEYILFDEPEPHRRMARLMKNIRRLDGARSDIYHPTLVPLEDKRIAGCLLHCVYAKNHAIDHMGWPTLDGLVQFYSEGVHEHGFFMATLRSVNLCLRAMTTKYRVNRQALPQKGESCDLAFDVFDCISDQITGYCLDQYSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1P2</accession>
    <name>A0A6I8V1P2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6896696</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_002136910.3">
        <property type="nucleotide sequence ID" value="XM_002136874.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1P2"/>
    <dbReference type="FunCoup" id="A0A6I8V1P2">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896696"/>
    <dbReference type="InParanoid" id="A0A6I8V1P2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="436"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002136910.3"/>
        </source>
    </evidence>
    <sequence length="567" mass="65274" checksum="C5FB958C6F9D3C70" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V231</accession>
    <name>A0A6I8V231_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Trypsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aqrs</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_002137001.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V231"/>
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        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896834"/>
    <dbReference type="InParanoid" id="A0A6I8V231"/>
    <dbReference type="OMA" id="YYNRAHR"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248361">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF94">
        <property type="entry name" value="AT20289P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026235687" evidence="8">
        <location>
            <begin position="22"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="53"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002137037.3"/>
        </source>
    </evidence>
    <sequence length="330" mass="37832" checksum="A8BE12668CC836F1" modified="2020-08-12" version="1">MWCKSIYLLLLLGLQCLQINATRLKQYKENYHRPVYYNPSHSKANHVDYNREALDARDKLDKEAVERTVEFDASVDKTYYMHIYYKGTVFCPGALISRRMIITSARCFMPPTTEPVREYKARYMYVLTGVDIGHATTTTPHAVNAFYMPGHKENGTSNDIALISLAKKLPRDTYQYIKLYRGIPKAGDRVKMAFLDPSEYEITLYDTKVVDMDRCKTTYEMMGLNEFIMEPEYYCVSNLKHNLNTACSTRPGDPLIIENQLAAINIFGENCDEVEDTDTMDIYFPIKHTIKFIQLATDALRAFTGTGPFNESVQTTTLSAIALAMQKEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3B4</accession>
    <name>A0A6I8V3B4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Importin-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6896971</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137156.2">
        <property type="nucleotide sequence ID" value="XM_002137120.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3B4"/>
    <dbReference type="FunCoup" id="A0A6I8V3B4">
        <property type="interactions" value="1653"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896971"/>
    <dbReference type="InParanoid" id="A0A6I8V3B4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002137156.2"/>
        </source>
    </evidence>
    <sequence length="1066" mass="118674" checksum="87BDDFA82D4F28F4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3H5</accession>
    <name>A0A6I8V3H5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Salivary glue protein Sgs-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137284.3">
        <property type="nucleotide sequence ID" value="XM_002137248.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3H5"/>
    <dbReference type="KEGG" id="dpo:6897100"/>
    <dbReference type="InParanoid" id="A0A6I8V3H5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026346025" description="Salivary glue protein Sgs-5" evidence="1">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137284.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12666" checksum="E3EFEFFD3D5BF04D" modified="2020-08-12" version="1">MKSFLSVCVLLALALSQVKAKCSGDCPDTEEVVWALSPGGGGCSVFRNKCYFDKANCTRKPPLTITTKEECQKKCADICPAIYQPTFGIYKGQTRNFGNECEKIVYTCRTGETFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4T7</accession>
    <name>A0A6I8V4T7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138894.3">
        <property type="nucleotide sequence ID" value="XM_002138858.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4T7"/>
    <dbReference type="KEGG" id="dpo:6898929"/>
    <dbReference type="InParanoid" id="A0A6I8V4T7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138894.3"/>
        </source>
    </evidence>
    <sequence length="145" mass="16560" checksum="A47E5915E8D24BF1" modified="2020-08-12" version="1">MCCPRMIACILYLSAAFNMLTAFVLLVLCVLAGRSRPGGKLNAILFLIWMLIHIPLILGVLQRKRALMWAYIIAWPLFFSCVTILLLLIYCSVIVWYDFLRFCWPLWFFVVWFVTAIPSWILAISYLSTGHDISTEASSTNSAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V516</accession>
    <name>A0A6I8V516_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Patched domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ptr</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138974.2">
        <property type="nucleotide sequence ID" value="XM_002138938.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V516"/>
    <dbReference type="FunCoup" id="A0A6I8V516">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="6899047"/>
    <dbReference type="KEGG" id="dpo:6899047"/>
    <dbReference type="InParanoid" id="A0A6I8V516"/>
    <dbReference type="OMA" id="MWHITFF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245498">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000013">
        <property type="entry name" value="PaTched Related family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000028">
        <property type="entry name" value="PaTched Related family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051697">
        <property type="entry name" value="Patched_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003392">
        <property type="entry name" value="PTHD_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10796">
        <property type="entry name" value="PATCHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10796:SF92">
        <property type="entry name" value="PATCHED-RELATED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02460">
        <property type="entry name" value="Patched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="328"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="705"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="732"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="758"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="801"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="833"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="10">
        <location>
            <begin position="270"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="922"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1013"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1045"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1071"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="936"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="959"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1156"/>
            <end position="1179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002138974.2"/>
        </source>
    </evidence>
    <sequence length="1187" mass="135016" checksum="EFA168AEE3C03984" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V846</accession>
    <name>A0A6I8V846_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">CD109 antigen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">TEP1-F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">Tep2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">MV-25-SWS-2005</strain>
            <tissue evidence="25">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modulates negatively TGFB1 signaling in keratinocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer; disulfide-linked. Interacts with TGFB1 and TGFBR1. Forms a heteromeric complex with TGFBR1, TGFBR2 and TGFBR3 in a ligand-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035836.2">
        <property type="nucleotide sequence ID" value="XM_015180350.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V846">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V846"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V846">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1940:FF:000003">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2950:FF:000001">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.690:FF:000003">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.20.50.160:FF:000002">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0882">Thioester bond</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026003561" description="CD109 antigen" evidence="20">
        <location>
            <begin position="20"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="21">
        <location>
            <begin position="439"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="22">
        <location>
            <begin position="693"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="23">
        <location>
            <begin position="1312"/>
            <end position="1402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_015035836.2"/>
        </source>
    </evidence>
    <sequence length="1424" mass="157822" checksum="99F6912B02D7CA2E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8Q2</accession>
    <name>A0A6I8V8Q2_DROPS</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4818038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035538.2">
        <property type="nucleotide sequence ID" value="XM_015180052.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035539.2">
        <property type="nucleotide sequence ID" value="XM_015180053.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
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        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="55"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="524"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015035538.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015035539.2"/>
        </source>
    </evidence>
    <sequence length="603" mass="67205" checksum="C99F5EAE2501C6E9" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8V9R9</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC6902667</name>
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    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_015181272.2"/>
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        <property type="component" value="Chromosome 4"/>
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    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015036758.2"/>
        </source>
    </evidence>
    <sequence length="101" mass="11175" checksum="8FA70890EDDA325F" modified="2020-08-12" version="1">MWSPQSYASTNWCRSGLLISFVDPHFQSLYESQSGDSPVRHLQPGSAHSVALCGWPGQHAQLLCQVDTSRAASFVVAPRDLRQQCAQPQPRRRIGEPSAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAA6</accession>
    <name>A0A6I8VAA6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037073.2">
        <property type="nucleotide sequence ID" value="XM_015181587.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAA6"/>
    <dbReference type="KEGG" id="dpo:26532147"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015037073.2"/>
        </source>
    </evidence>
    <sequence length="99" mass="10358" checksum="60F65610E229A3BA" modified="2020-08-12" version="1">MKKLGMQTKDAGSGSSRNMKFDIIKGHLCVKCNTAAAAAAVAVGRGRTTKKSTAHLNVIEIEAHLTSNAGATDPRPHSHSLPTLSLSQPNTHTSRGGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAU7</accession>
    <name>A0A6I8VAU7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Kinesin-like protein KIF19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Kif19A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037333.2">
        <property type="nucleotide sequence ID" value="XM_015181847.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAU7"/>
    <dbReference type="FunCoup" id="A0A6I8VAU7">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801634"/>
    <dbReference type="InParanoid" id="A0A6I8VAU7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01370">
        <property type="entry name" value="KISc_KIP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000205">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="11">
        <location>
            <begin position="70"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="700"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="568"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="717"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="148"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015037333.2"/>
        </source>
    </evidence>
    <sequence length="784" mass="86933" checksum="EBE12D3DA0260BF3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VC12</accession>
    <name>A0A6I8VC12_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Two pore potassium channel protein sup-9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Task6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037539.1">
        <property type="nucleotide sequence ID" value="XM_015182053.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VC12"/>
    <dbReference type="FunCoup" id="A0A6I8VC12">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="GeneID" id="4802009"/>
    <dbReference type="InParanoid" id="A0A6I8VC12"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081917">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VC12">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000090">
        <property type="entry name" value="two pore potassium channel protein sup-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF326">
        <property type="entry name" value="ACID-SENSITIVE TWO PORE DOMAIN K+ CHANNEL DTASK-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038061">
        <property type="entry name" value="K_channel_subfamily_K_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="14">
        <location>
            <begin position="68"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="14">
        <location>
            <begin position="169"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015037539.1"/>
        </source>
    </evidence>
    <sequence length="418" mass="46819" checksum="EE406A51B6697FEF" modified="2020-08-12" version="1">MKKQNVRTISLIVCTFTYLLVGAAVFDALESETEKRRWEALQDAEDMIIRKYNISPEDFKVMETVVLKSESHKAGQQWKFTGAFYYATTVLTTIGYGHSTPSTVGGKLFTMCYAIVGIPLGLVMFQSIGERVNRLSSYVIQAVRTSLRCKRTVASEVDLICVVTTLSSLTIAGGAAAFSKFEGWSYFDSVYYCFITLTTIGFGDMVALQRDNALNRKPEYVMFALIFILFGLAIVAASLNLLVLRFVTMNTEDERRDEAQAMQFMSKSSLWTNEALQVAVKLEGDVITSNGSILSGYEGHDAQSLNGSSASSMCSCHCMCLNRNRHKKSNHLGRNNDTENRYRLQRSPTHIRHLLPEVVPMQDLNYDYDTQSLHSMPDRGIMDSNYMGVDMVDTASSGSPLRPQQLLKRNVSLLSFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VD68</accession>
    <name>A0A6I8VD68_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acid phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Acph-1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_015038824.2">
        <property type="nucleotide sequence ID" value="XM_015183338.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VD68"/>
    <dbReference type="FunCoup" id="A0A6I8VD68">
        <property type="interactions" value="680"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VD68"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VD68">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF211">
        <property type="entry name" value="PROSTATIC ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00778">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="425"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015038824.2"/>
        </source>
    </evidence>
    <sequence length="465" mass="53038" checksum="5570448347935E7E" modified="2020-08-12" version="1">MYILSCSWSRTLLTSLALKMWNQIQPVCCAKALVVIGVICVLSFGIGNAVHIPAYGSSEGATRPPDQLATLPGQLKFAHVIFRHGDRTPVDPYPTDPWNNIKFWPTGWGQLTNRGKEQHYELGKWLRNRYQSLLSSRYTNEDIFVQSTDVDRALMSAQSALSGLYEPQGNDIWNPRIDWQPIPVHTIPEKDDSILAMKASCPAYDYELATQEASSEFQALNERYRELFFYLSQNSGRLVKSFIEAQYLNNTLFIEKLYNMTLPVWTEKVYGRADLTYVSNFAFSIGTYTRSLARLKAGPLLKDIFERFDKKFNSQLTPDRSVWIYSAHDTTIANILNSLKLFELHSPPYAACIMLEMRVDDSNTPLVSVFYKNTTAEPLPLDIPGCGLSCPLKTLVNLYQDVLPKNWESECKRSTMMMTYEEANLGAATAILIFIITVLLCASYGLMIYYRRRNYRLYTSYSPMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDL8</accession>
    <name>A0A6I8VDL8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">Tsp42Ef</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015039090.1">
        <property type="nucleotide sequence ID" value="XM_015183604.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039091.1">
        <property type="nucleotide sequence ID" value="XM_015183605.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDL8"/>
    <dbReference type="SMR" id="A0A6I8VDL8"/>
    <dbReference type="GeneID" id="4803458"/>
    <dbReference type="KEGG" id="dpo:4803458"/>
    <dbReference type="OMA" id="CRLCNSI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071899">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF551">
        <property type="entry name" value="RE08073P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="134"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015039090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015039091.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24787" checksum="986EF60DC902A5CA" modified="2020-08-12" version="1">MSSTSALKVFVYALDIVCALLALVLISFGIYVVVSYDLNEIGGLSAYAFIYLGAVALVILLWGYLSAWKESVCCTVTFVVFLWLIIATQFAVVFLLIHNESSVASNLANPIEATWEEELNSPGAMSIYQNWFQCCGRGSPQDYIVNERLPPETCFRNHDKSKAENLIPNGCRKEFENYWLQLLQIFNILGCVLIGIELLISVISIRLCNSIRNDQRRSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFI6</accession>
    <name>A0A6I8VFI6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26533374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040062.2">
        <property type="nucleotide sequence ID" value="XM_015184576.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFI6"/>
    <dbReference type="KEGG" id="dpo:26533374"/>
    <dbReference type="InParanoid" id="A0A6I8VFI6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015040062.2"/>
        </source>
    </evidence>
    <sequence length="102" mass="11897" checksum="CD101235A07A2395" modified="2020-08-12" version="1">MDKRREALTFRVLQRSRDFPALLPVCMVLDRSYIYDRVHRVLYLSALTCSIGRDSLWTQTALPRSFLLPRLQVPLRRQGMTLACPRTPICAPPFITSVFRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIM6</accession>
    <name>A0A6I8VIM6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Regulatory-associated protein of mTOR-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4814522</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041714.2">
        <property type="nucleotide sequence ID" value="XM_015186228.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VIM6">
        <property type="interactions" value="1484"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VIM6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VIM6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000276">
        <property type="entry name" value="Regulatory-associated protein of mTOR isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Raptor N-terminal CASPase-like" evidence="5">
        <location>
            <begin position="190"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="25"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1120"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1177"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1213"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1305"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1411"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1129"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1184"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1229"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1305"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1318"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1428"/>
            <end position="1445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015041714.2"/>
        </source>
    </evidence>
    <sequence length="1683" mass="181896" checksum="5195B46D0221136D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNI3</accession>
    <name>A0A6I8VNI3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LOW QUALITY PROTEIN: uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232616.1">
        <property type="nucleotide sequence ID" value="XM_033376725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNI3"/>
    <dbReference type="KEGG" id="dpo:4802151"/>
    <dbReference type="InParanoid" id="A0A6I8VNI3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006631">
        <property type="entry name" value="DM4_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398:SF23">
        <property type="entry name" value="AGAP002978-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398">
        <property type="entry name" value="AGAP007094-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07841">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00718">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026078569" evidence="1">
        <location>
            <begin position="36"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232616.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="42235" checksum="17EE89430B6FD8C8" modified="2020-08-12" version="1">MTHLSAWTSAATMLMLQLAVPLLLLMVQQFSTCSAVSHKVDNFDGLAEASMTGSRYREHNENSFDMHYDNSSPDSSPSQLLANNGTRILRRGKRYLQFSKGSRMSWRTNGKNTLLTINTLYAYGYGFRTNYPFPSIEEQKKDNAVFFRLFKRDLFSKLETALDGHGFDGRACMLKSFCTAVLDVDKSEQKSGMLFKLLKLVFSRAKRYLDIIETTRIFVGTQIFNLGNRSLLIPKRFSHFSIKFRVNAKNNVIASPIVWAHGYGFRANTPVLVKRENRPFRRDTYELLHELIDRSGFDGRACVLKAYCTALAGDHGQGFLFKLLKYVFTLDEHEKRHMPHLREENCEQIMHSHCPLSFDSISPYTDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNR4</accession>
    <name>A0A6I8VNR4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18 19">Dynein heavy chain 5, axonemal isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18 19" type="primary">Dhc1</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18 19">MV-25-SWS-2005</strain>
            <tissue evidence="18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232709.1">
        <property type="nucleotide sequence ID" value="XM_033376818.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232710.1">
        <property type="nucleotide sequence ID" value="XM_033376819.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VNR4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001221">
        <property type="entry name" value="Axonemal dynein heavy chain 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000004">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000002">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000003">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000044">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001547">
        <property type="entry name" value="Dynein heavy chain, cytosolic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000003">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000003">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000320">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000017">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056759">
        <property type="entry name" value="DYH2-5-8_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF4">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25007">
        <property type="entry name" value="DYH2-5-8_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2051"/>
            <end position="2198"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2333"/>
            <end position="2462"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2710"/>
            <end position="2883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="106"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="954"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3336"/>
            <end position="3363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3556"/>
            <end position="3604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="977"/>
            <end position="990"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033232709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033232710.1"/>
        </source>
    </evidence>
    <sequence length="4751" mass="544475" checksum="10B2774B2B3CEE99" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPZ7</accession>
    <name>A0A6I8VPZ7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6896910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232943.1">
        <property type="nucleotide sequence ID" value="XM_033377052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPZ7"/>
    <dbReference type="KEGG" id="dpo:6896910"/>
    <dbReference type="InParanoid" id="A0A6I8VPZ7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026271331" description="MD-2-related lipid-recognition domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21075" checksum="46C806F26908FC53" modified="2020-08-12" version="1">MRVPSIPIRVSTFVALILILHGSDGLFKYKNIKCKCYEKSYCEFKKCELKVLGRGIVGLNMHVQAKQLPITSVKAILTTFRQFSGYRPFLYNVTVDICGFLKNPKRFPFFSMVHDAIRNFSNFNHSCPYNDDIIIDRMVLNDEMISRMPVPNGFYKLLVAVATEGIWRGEVEAYVEVNLGYDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTI6</accession>
    <name>A0A6I8VTI6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-hexosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fdl</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="7"/>
    <dbReference type="RefSeq" id="XP_033234228.1">
        <property type="nucleotide sequence ID" value="XM_033378337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTI6"/>
    <dbReference type="FunCoup" id="A0A6I8VTI6">
        <property type="interactions" value="1492"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805465"/>
    <dbReference type="InParanoid" id="A0A6I8VTI6"/>
    <dbReference type="OMA" id="ENERCIR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081336">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016231">
        <property type="term" value="F:beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06562">
        <property type="entry name" value="GH20_HexA_HexB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000063">
        <property type="entry name" value="Beta-hexosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029019">
        <property type="entry name" value="HEX_eukaryotic_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="BETA-HEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600:SF3">
        <property type="entry name" value="BETA-HEXOSAMINIDASE FDL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14845">
        <property type="entry name" value="Glycohydro_20b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001093">
        <property type="entry name" value="B-hxosamndse_ab_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase eukaryotic type N-terminal" evidence="11">
        <location>
            <begin position="98"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="10">
        <location>
            <begin position="271"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001093-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033234228.1"/>
        </source>
    </evidence>
    <sequence length="655" mass="74490" checksum="446E38B161673B50" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVS2</accession>
    <name>A0A6I8VVS2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26532503</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235190.1">
        <property type="nucleotide sequence ID" value="XM_033379299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VVS2"/>
    <dbReference type="KEGG" id="dpo:26532503"/>
    <dbReference type="InParanoid" id="A0A6I8VVS2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033235190.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="17191" checksum="5398B5ED87D031D3" modified="2020-08-12" version="1">MLMATAIGPSISPIWQELDGEIGVAAAGEAALSEAWQRQPVVWMLLATAIGPSNFAHLAGIGWRNWCGNCWRGSSIGGVAGTYVLTAACGLDVVGDGDRAFKFRPFGRNWMAKLVWQLLERQLYRRRGSGCSGPVRWIVRSVVNKSLNVVQLASDVWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYU9</accession>
    <name>A0A6I8VYU9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycoprotein endo-alpha-1,2-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4818078</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 99 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236275.1">
        <property type="nucleotide sequence ID" value="XM_033380384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYU9"/>
    <dbReference type="FunCoup" id="A0A6I8VYU9">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4818078"/>
    <dbReference type="InParanoid" id="A0A6I8VYU9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VYU9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026071">
        <property type="entry name" value="Glyco_Hydrolase_99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572">
        <property type="entry name" value="ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572:SF4">
        <property type="entry name" value="RE57134P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16317">
        <property type="entry name" value="Glyco_hydro_99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033236275.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="55042" checksum="0393E47F0B39D3F1" modified="2020-08-12" version="1">MLLKKYVKTRRKVKIVLLLAIVGLLVITIIVLGSTGRNAAIELLLDERFIARAYRPGDKHQQQQQPLQEQKQPPVAAVGLLQPQRENGVIVPEKYDEHKIKARIIQNRLDQIKQQQQHDHEAEQSRTTLEVATTAVHIFYTAPVPWYKSKKPPPPPAEHPNDMPLTTTPKAVRILNTAFYPALGLYKPSVSLLSQHFENIRSCGIGVLILSFTGKPAETTLLHQIIDLAPQHNLTVTFELSVAGNQSVEYVRQQLQEIAGFASLPGFYRVWSQSRGAAMPVLYVSNAYKLSDSLGRLLCRTPSQVDPDGLRRVLDGFFIGHIRLKSHADVMRRLCFDGFYSKLPSNGAVFASTWKNWSYLKSYAQSYKMMFVPTVGPGFAERNKFPRHGDIQRHRSNGRYYGVAWRTAILNHVGFINIASYNNWPDGSQIEEVMPRAGFLDYNPGSRTKYLDLTAHWVGNFLKTRQEAAAAAAPASPQNCYELLNGTIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZK5</accession>
    <name>A0A6I8VZK5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein dpr3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dpr3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235944.1">
        <property type="nucleotide sequence ID" value="XM_033380053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZK5"/>
    <dbReference type="FunCoup" id="A0A6I8VZK5">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817542"/>
    <dbReference type="InParanoid" id="A0A6I8VZK5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000129">
        <property type="entry name" value="CLUMA_CG018772, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF4">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 2, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="280"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="386"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235944.1"/>
        </source>
    </evidence>
    <sequence length="562" mass="61206" checksum="8E6E41DE232EF502" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0H5</accession>
    <name>A0A6I8W0H5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">BK channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Maxi K channel</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">slo</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. Slo sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236836.1">
        <property type="nucleotide sequence ID" value="XM_033380945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0H5"/>
    <dbReference type="FunCoup" id="A0A6I8W0H5">
        <property type="interactions" value="411"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W0H5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W0H5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000035">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001832">
        <property type="entry name" value="Calcium-activated potassium channel slowpoke-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="19">
        <location>
            <begin position="356"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="19">
        <location>
            <begin position="876"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="808"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1216"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="808"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_033236836.1"/>
        </source>
    </evidence>
    <sequence length="1246" mass="138463" checksum="3CD594298425ADC7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1Y8</accession>
    <name>A0A6I8W1Y8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237365.1">
        <property type="nucleotide sequence ID" value="XM_033381474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W1Y8"/>
    <dbReference type="FunCoup" id="A0A6I8W1Y8">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W1Y8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W1Y8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033237365.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="26850" checksum="9954ED14FDBAD1DF" modified="2020-08-12" version="1">MMKSNSCIVALPQRNWHTLLLKNSETGELEYFYTDSSRVTQPAYDGETGEPVHGQMKVRYRKHHNFHVPKHCLRGTLCDEMDNLMTNKYQGDLDITVQHGARKEPKKTTPGYEREDYCHMDGVSSNIILGYERQHYLLFLVPTLFCYNFFIGALMALIEIFLHMMSHHKNGLTMQKTLYFRSPLHVLSSQFCAICRTESDSKYNRIFDILNKQMRTAHRSATLKNMAKAMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W234</accession>
    <name>A0A6I8W234_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26533006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237387.1">
        <property type="nucleotide sequence ID" value="XM_033381496.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:26533006"/>
    <dbReference type="InParanoid" id="A0A6I8W234"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W234">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033237387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="35556" checksum="37C2BF5770523EEB" modified="2020-08-12" version="1">METQRTITDLPFEVLDLIFKQLDYPRQEWQLVHAHEILAEEFAYESRDDCRKLSLDYWWTPDTYALIWKTCGSSVRELDYHKGSDELAKHCPHLESIDFCVDSRYWQHVKSLILLLKGNNTIRSTVIDVDDKNIPFTILHGLTEFPQLRKLVFVHYIGDAGSLFNAMACMNWSHLSTEIPVCPKLKSFTLTSCECHIKNLVCQTILKQSNSLEYLDVYCRLSAFDGDGFFAVLRACKKLRKFRDPLGDVTIYRAYVFAMVDILRENGFTPEKPFKLWLFDRPQMRWIKRLVRQNHLWPSYDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4D0</accession>
    <name>A0A6I8W4D0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyridoxal-dependent decarboxylase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4814690</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238182.1">
        <property type="nucleotide sequence ID" value="XM_033382291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4D0"/>
    <dbReference type="FunCoup" id="A0A6I8W4D0">
        <property type="interactions" value="2140"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814690"/>
    <dbReference type="InParanoid" id="A0A6I8W4D0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000156">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055103">
        <property type="entry name" value="PDXDC1-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055102">
        <property type="entry name" value="PDXDC1-like_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF1">
        <property type="entry name" value="PYRIDOXAL-DEPENDENT DECARBOXYLASE DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22930">
        <property type="entry name" value="PDXDC1-like_cen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22937">
        <property type="entry name" value="PDXDC1-like_cen2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDXDC1/PDXD2 second" evidence="8">
        <location>
            <begin position="457"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="PDXDC1-like third" evidence="9">
        <location>
            <begin position="572"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="61"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238182.1"/>
        </source>
    </evidence>
    <sequence length="861" mass="92734" checksum="2E84B2D632C43ED7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W534</accession>
    <name>A0A6I8W534_DROPS</name>
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        <recommendedName>
            <fullName evidence="5">[heparan sulfate]-glucosamine N-sulfotransferase</fullName>
            <ecNumber evidence="5">2.8.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">sfl</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">MV-25-SWS-2005</strain>
            <tissue evidence="25">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; heparin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sulfotransferase 1 family. NDST subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.8" evidence="5"/>
    <dbReference type="RefSeq" id="XP_033238480.1">
        <property type="nucleotide sequence ID" value="XM_033382589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W534"/>
    <dbReference type="FunCoup" id="A0A6I8W534">
        <property type="interactions" value="1237"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814130"/>
    <dbReference type="InParanoid" id="A0A6I8W534"/>
    <dbReference type="UniPathway" id="UPA00756"/>
    <dbReference type="UniPathway" id="UPA00862"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019213">
        <property type="term" value="F:deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015016">
        <property type="term" value="F:heparan sulfate N-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030210">
        <property type="term" value="P:heparin proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000176">
        <property type="entry name" value="bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021930">
        <property type="entry name" value="Heparan_SO4_deacetylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056793">
        <property type="entry name" value="HSNSD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF56">
        <property type="entry name" value="BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12062">
        <property type="entry name" value="HSNSD-CE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25119">
        <property type="entry name" value="HSNSD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Heparan sulfate-N-deacetylase N-terminal" evidence="23">
        <location>
            <begin position="236"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Heparan sulphate-N-deacetylase deacetylase" evidence="22">
        <location>
            <begin position="461"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="21">
        <location>
            <begin position="755"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="99"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="16">
        <location>
            <position position="764"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="880"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="985"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="1001"/>
            <end position="1005"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="986"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF12062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF25119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_033238480.1"/>
        </source>
    </evidence>
    <sequence length="1051" mass="118071" checksum="734CAE1F9695B9B0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5P6</accession>
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        <submittedName>
            <fullName evidence="3">RNA polymerase II degradation factor 1 isoform X1</fullName>
        </submittedName>
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        <name evidence="3" type="primary">LOC6900836</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238607.1">
        <property type="nucleotide sequence ID" value="XM_033382716.1"/>
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        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="736"/>
        </location>
    </feature>
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        <location>
            <begin position="8"/>
            <end position="31"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="177"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="699"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238607.1"/>
        </source>
    </evidence>
    <sequence length="773" mass="86409" checksum="D6FF840CF34CD9CA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7W2</accession>
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        <submittedName>
            <fullName evidence="10">Transcription factor Ouib-like isoform X1</fullName>
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    </protein>
    <gene>
        <name evidence="10" type="primary">LOC26532315</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239453.1">
        <property type="nucleotide sequence ID" value="XM_033383562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W7W2"/>
    <dbReference type="KEGG" id="dpo:26532315"/>
    <dbReference type="InParanoid" id="A0A6I8W7W2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="8">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="145"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033239453.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="35128" checksum="F8AD3F15C88F07CC" modified="2020-08-12" version="1">MNNQCRACGSAMYNTKGRNLFQKKNAKLLLNFNLVTNNLALKKDPRVPSCICASCAESLNRAVIFRARALETQENFLQQRAGLPENDLPTDDETPGETSNSEVKAQDADSLMSGLENELGGKTGFDGTDAYETAQVADSLITSLQKDSGHEKKKSSITENIIKAPKQLADNSTFRSSPSTTPTSRSPLSTTPTSSSTRPSRLTSKNAAQSDKRHPLPKKILKRRSCTMNKLKCKFCEQTFVSPFELSCHMRSHTAETSCKCSYCDRAFSTKGNTIRHERTHTRQAAFVCPTCDMSFTLATVLKRHISLYHTQMSMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9I1</accession>
    <name>A0A6I8W9I1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117184904</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239937.1">
        <property type="nucleotide sequence ID" value="XM_033384046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9I1"/>
    <dbReference type="KEGG" id="dpo:117184904"/>
    <dbReference type="InParanoid" id="A0A6I8W9I1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239937.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="43883" checksum="3198815F4777FB1B" modified="2020-08-12" version="1">MSDLVGTEEFSAAQLREWLESLNLPKGGSKAAMAARLNEIPVELRGQGPPAAETCENEREDEAAADQDSTKSERKEKNEKAPQAPGHNNNHGEYRAEVEMLKLQIELLKLQSEREGRREGRGENTTPAANNDAGVMLLNAAKDMLPTYHGNISGNNDDVTTWIAQFKAVAKVNKLKDEKLLMLLMSKLKDKALVWLHSSPEHMSLPIDQLLNVMEDTFHPKESKLLLRRKFESRSWARGEEFSMYFNAKVSLASRIVIDDEEFIDGVIEGIPDVGLRRQAHMQCFGAPYQLLKAFEKIMLPKKYGSTEGANTGASPTPIRCYNCNSLGHVAGECRKPKRERGACYGCGSMSHRDTWCHCVTVTKRSTRLPHRLREPNKFYEVIVSRAPFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9I9</accession>
    <name>A0A6I8W9I9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6 7 8" type="primary">LOC4814644</name>
    </gene>
    <organism evidence="3 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
            <tissue evidence="4 5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354749.4">
        <property type="nucleotide sequence ID" value="XM_001354713.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041643.2">
        <property type="nucleotide sequence ID" value="XM_015186157.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041644.2">
        <property type="nucleotide sequence ID" value="XM_015186158.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240052.1">
        <property type="nucleotide sequence ID" value="XM_033384161.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240053.1">
        <property type="nucleotide sequence ID" value="XM_033384162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9I9"/>
    <dbReference type="KEGG" id="dpo:4814644"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055121">
        <property type="entry name" value="HTH_69"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705">
        <property type="entry name" value="AGAP000321-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47705:SF1">
        <property type="entry name" value="PNP_UDP_1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22979">
        <property type="entry name" value="HTH_69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn-helix" evidence="2">
        <location>
            <begin position="296"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354749.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015041643.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015041644.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033240052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033240053.1"/>
        </source>
    </evidence>
    <sequence length="708" mass="78569" checksum="896B79F3BA853638" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9T1</accession>
    <name>A0A6I8W9T1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox protein unc-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">OdsH</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the paired homeobox family. Unc-4 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240150.1">
        <property type="nucleotide sequence ID" value="XM_033384259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9T1"/>
    <dbReference type="FunCoup" id="A0A6I8W9T1">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901808"/>
    <dbReference type="InParanoid" id="A0A6I8W9T1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000057">
        <property type="entry name" value="Short stature homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46799">
        <property type="entry name" value="HOMEOBOX PROTEIN UNC-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46799:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN UNC-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="197"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="199"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="133"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="254"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="368"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033240150.1"/>
        </source>
    </evidence>
    <sequence length="432" mass="48510" checksum="49B5B4EBB9DA78C5" modified="2020-08-12" version="1">MDHLIKQLQLAAAARAQAHIHSSMAPHSHSHPHPHSHPHPHAASALQLYAAAVNMRVPGWPPFLGLPPPMTDTDATTTAGSVSTSSSSVYMLRQMALMQRANAAAAAAALQQQQQQHQQPHDQEFAIKLEHQEERTSQRKDQGACSRQRMHVNDESPHHQPSSGQEEDETASEESSNARHDPDPDPDPSPDADGDASKRRRCRTNFSSWQLEELERAFLSSHYPDIFMREALAMRLDLKEGRIAVWFQNRRAKWRKKEHTKKGPGRPAHNAQPQSCSGAPIPLSELRARERAQRCKRMGKSIERQARKLRLKGIEVNMERLRADYLAAHKDDWQELELGLGLGLEHGYDDDEAEDVEEGHDDELPIDVVSVGNSQDNSYSSSRTYQRSIGSEPDEHRSRVMIKLEPTPKVPLPSQHKGLYNSPFSIESLLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAA8</accession>
    <name>A0A6I8WAA8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine-rich repeat extensin-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239484.1">
        <property type="nucleotide sequence ID" value="XM_033383593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WAA8"/>
    <dbReference type="KEGG" id="dpo:4812459"/>
    <dbReference type="InParanoid" id="A0A6I8WAA8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026112962" evidence="2">
        <location>
            <begin position="21"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239484.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="38161" checksum="33BC33250D67C882" modified="2020-08-12" version="1">MKPMLILLALAGLAAVCVRADVSHLFGGGGHGHHHEHDHHDHHDHHDHHDHHDYHYDAHAHSSHASHQDGHHDLHIDLHYQPEPHHHHHHVEQEPKFNIPSGYSYGGPPPAPKSSYLPPAEKPRQKYLPPKASLPPPPPPPAPKTSYLPPSKPEVKYLPPAPAPAPAPKYLPPAPAPAPKYLPPAPAPAPKYLPPAPAPAPAPKYLPPKVAPSLPPPPPPPAVAPKPSYLPPAKPEKSYLPPAPPASKYLPAEEPAARYLPPKVAPSLPPPPPPPAVAPKASYLPPAPRPESSYLPPSAEHHHHQHQHQPEIDFHIPIPSYALPLQSENHVHVPEQGYQYKPPLRRFRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAE8</accession>
    <name>A0A6I8WAE8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4814952</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KCMF1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="RefSeq" id="XP_033240375.1">
        <property type="nucleotide sequence ID" value="XM_033384484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WAE8"/>
    <dbReference type="FunCoup" id="A0A6I8WAE8">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814952"/>
    <dbReference type="InParanoid" id="A0A6I8WAE8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02338">
        <property type="entry name" value="ZZ_PCMF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="9">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033240375.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="22283" checksum="9F83C6C486B000FE" modified="2020-08-12" version="1">MDRQHGATCDGCDLWGNGITGCRYKCLKCADFDLCKSCYDAKVVSGRHKSEHPMQCLLDEDAKKLYFAGEPIPYLNADSFTCPICGTMGHSYHELVKHVVAQHKDDRSDAICPLCVALPMAPPDRLLKISQHFRRQHRESSNIRDAVAEEESRASDMADRFSRLSLAEAARSGLPIRHLLVRVTRPANRLPPDTSSEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBF7</accession>
    <name>A0A6I8WBF7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Octopamine receptor Oamb isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Oamb</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240741.1">
        <property type="nucleotide sequence ID" value="XM_033384850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WBF7"/>
    <dbReference type="FunCoup" id="A0A6I8WBF7">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:13036427"/>
    <dbReference type="InParanoid" id="A0A6I8WBF7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WBF7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15063">
        <property type="entry name" value="7tmA_Octopamine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000369">
        <property type="entry name" value="octopamine receptor Oamb isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF174">
        <property type="entry name" value="TYRAMINE_OCTOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="541"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="37"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="347"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="465"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="609"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="617"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033240741.1"/>
        </source>
    </evidence>
    <sequence length="633" mass="70732" checksum="EC9B3DC3AEC41213" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDM7</accession>
    <name>A0A6I8WDM7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine protease inhibitor 42Dd-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC117185204</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241493.1">
        <property type="nucleotide sequence ID" value="XM_033385602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDM7"/>
    <dbReference type="KEGG" id="dpo:117185204"/>
    <dbReference type="InParanoid" id="A0A6I8WDM7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19954">
        <property type="entry name" value="serpin42Dd-like_insects"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF372">
        <property type="entry name" value="ACCESSORY GLAND PROTEIN ACP76A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="56"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033241493.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="46710" checksum="5F51A7AD1E07A24F" modified="2020-08-12" version="1">MMGRTHQRSSGHSSNYSSVLCRSPGSMTALSLLLLLGSLLTIVQTDTSTGDTKFALELLRALARDKSSPNIVFSPSSIRTALALAYLGAEGVTGEELKQTLSLEGSDKNDVAQRFAHLLAQEENQSEDDAQFSYANRIYVSERYRLIQAYQELAGKYFNASAENVNFADNFKVSQQINSWVATKTHDQIKDLISADSLSSETAAVLINAIYFKGKWENPFSETMTATHDFTTRFGEKVKTSFMFQWQFFRHAELPSLKATALEMRYRGTDIVLLIILPLEEQGLYALEEKISVLDLNEISSQMREEHVRLQMPKFKLEFEVSLKPVLQQLGIKTMFGPNADFSSLAKGRDIMISEVKHKAFMDVNENGTTAAASTYLLAMPHSGRKGQTIPFIVDHPFLFAIKDEQSIFFLGHVTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>B5DI54</accession>
    <accession>A0A6I8UXD2</accession>
    <name>B5DI54_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Actin-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6902854</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132590.1">
        <property type="nucleotide sequence ID" value="XM_002132554.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DI54"/>
    <dbReference type="SMR" id="B5DI54"/>
    <dbReference type="STRING" id="46245.B5DI54"/>
    <dbReference type="GeneID" id="6902854"/>
    <dbReference type="KEGG" id="dpo:6902854"/>
    <dbReference type="eggNOG" id="KOG0677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_0_0_1"/>
    <dbReference type="InParanoid" id="B5DI54"/>
    <dbReference type="OMA" id="MAFNVIQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247289">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DI54">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10220">
        <property type="entry name" value="ASKHA_NBD_Arp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000005">
        <property type="entry name" value="Actin-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002132590.1"/>
        </source>
    </evidence>
    <sequence length="406" mass="45705" checksum="6E767707D3CCEA44" modified="2008-10-14" version="1">MDTKGRHIIVCDNGTGSVKCGLAGSNLPAHIFPSIVGRPILRAMNTCDANGVQMDDVMVGDEALKLRSLLAVSHPMENGIIRNWEDMCHVWDYTFGPKKMNIEPANSKILLAEPPMIPTRYREKMMEVMFEHYGFDATYLASQAVLTLYGQGLTTGAVIDAGDGVTNICAVYEEAALPHLTKRLNVCGRDITRRLVKLLMQRGYALNNSADFETVRLMKEKLCYVGYDIEQEKRLAEDTTALVESYTLPDGRVIKIGGERFEAPEAFFQPHLIDVEAAGLSEMAFNVIQAADIDIRPQLFRHIVLSGGSTMFPGFPSRLENDLKKLFLERVLQRDAENMPKFKIRIKDPPTRKNNVFNGGSVLANVTKDRDDFWMSKEEYEEQGLKVLDKLKQKSKKKEKYETDEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-24" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKS8</accession>
    <name>SWS_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neuropathy target esterase sws</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Swiss cheese</fullName>
            <ecNumber>3.1.1.5</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sws</name>
        <name type="ORF">GA22927</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. Plays a role in the signaling mechanism between neurons and glia that regulates glia wrapping during development of the adult brain. Essential for membrane lipid homeostasis and cell survival in both neurons and glia of the adult brain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with Pka-C3; interaction inhibits the catalytic function of Pka-C3 and the esterase activity of sws.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="2">Sws tethers Pka-C3 to the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5"/>
    <dbReference type="EMBL" id="CH379063">
        <property type="protein sequence ID" value="EDY72128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKS8"/>
    <dbReference type="SMR" id="B5DKS8"/>
    <dbReference type="FunCoup" id="B5DKS8">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DKS8"/>
    <dbReference type="eggNOG" id="KOG2968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_1_0_1"/>
    <dbReference type="InParanoid" id="B5DKS8"/>
    <dbReference type="OMA" id="GQQEDRH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034349">
        <property type="term" value="P:glial cell apoptotic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006643">
        <property type="term" value="P:membrane lipid metabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd07225">
        <property type="entry name" value="Pat_PNPLA6_PNPLA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000010">
        <property type="entry name" value="neuropathy target esterase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000122">
        <property type="entry name" value="Neuropathy target esterase sws"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000135">
        <property type="entry name" value="Neuropathy target esterase sws"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000022">
        <property type="entry name" value="Neuropathy target esterase sws"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000033">
        <property type="entry name" value="Neuropathy target esterase sws"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001423">
        <property type="entry name" value="LysoPLipase_patatin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01237">
        <property type="entry name" value="UPF0028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0442">Lipid degradation</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0524">Neurogenesis</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000389225" description="Neuropathy target esterase sws">
        <location>
            <begin position="1"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="3">
        <location>
            <begin position="57"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="944"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="362"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="422"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1367"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="4">
        <location>
            <begin position="948"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="975"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="1097"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="435"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1370"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1389"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1452"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1484"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="977"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="1097"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="176"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>a nucleoside 3',5'-cyclic phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="474"/>
            <end position="601"/>
        </location>
        <ligand>
            <name>a nucleoside 3',5'-cyclic phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="590"/>
            <end position="717"/>
        </location>
        <ligand>
            <name>a nucleoside 3',5'-cyclic phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="2">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9U969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EDY72128.1"/>
        </source>
    </evidence>
    <sequence length="1494" mass="168444" checksum="6F53A60DFB6E7999" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>B5DN54</accession>
    <accession>A0A6I8UZN4</accession>
    <name>B5DN54_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP-1 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AP-1gamma</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134234.1">
        <property type="nucleotide sequence ID" value="XM_002134198.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DN54"/>
    <dbReference type="FunCoup" id="B5DN54">
        <property type="interactions" value="1717"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DN54"/>
    <dbReference type="GeneID" id="6901560"/>
    <dbReference type="KEGG" id="dpo:6901560"/>
    <dbReference type="eggNOG" id="KOG1062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003824_0_0_1"/>
    <dbReference type="InParanoid" id="B5DN54"/>
    <dbReference type="OMA" id="XYAPSKR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247500">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000030">
        <property type="entry name" value="AP-1 complex subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017107">
        <property type="entry name" value="AP1_complex_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037094">
        <property type="entry name" value="AP1_complex_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002134234.1"/>
        </source>
    </evidence>
    <sequence length="965" mass="105645" checksum="A89796C0DE728387" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>B5DNA7</accession>
    <accession>A0A6I8UZK0</accession>
    <name>B5DNA7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Barrier-to-autointegration factor-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Barrier-to-autointegration factor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6901617</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134287.1">
        <property type="nucleotide sequence ID" value="XM_002134251.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DNA7"/>
    <dbReference type="SMR" id="B5DNA7"/>
    <dbReference type="STRING" id="46245.B5DNA7"/>
    <dbReference type="GeneID" id="6901617"/>
    <dbReference type="KEGG" id="dpo:6901617"/>
    <dbReference type="eggNOG" id="KOG4233">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167806_0_0_1"/>
    <dbReference type="InParanoid" id="B5DNA7"/>
    <dbReference type="OMA" id="YMVLGQF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247239">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.40:FF:000002">
        <property type="entry name" value="Barrier to autointegration factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.40">
        <property type="entry name" value="Barrier-to-autointegration factor, BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051387">
        <property type="entry name" value="BAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004122">
        <property type="entry name" value="BAF_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036617">
        <property type="entry name" value="BAF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47507">
        <property type="entry name" value="BARRIER TO AUTOINTEGRATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47507:SF6">
        <property type="entry name" value="BARRIER-TO-AUTOINTEGRATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02961">
        <property type="entry name" value="SAM_BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01023">
        <property type="entry name" value="BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47798">
        <property type="entry name" value="Barrier-to-autointegration factor, BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00079764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002134287.1"/>
        </source>
    </evidence>
    <sequence length="90" mass="10087" checksum="6744706E921275F0" modified="2008-10-14" version="1">MSGTSKKYSNFVAEPMGNKLVTELAGIGETLGGRLAEAGFDKAYTVLGQYLVLKKDEELFKYWMKDVCHASSKQASDCYNCLNDWCEEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>B5DP70</accession>
    <accession>A0A6I8V0A4</accession>
    <name>B5DP70_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese domain-containing protein CG4456</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134695.2">
        <property type="nucleotide sequence ID" value="XM_002134659.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DP70"/>
    <dbReference type="FunCoup" id="B5DP70">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DP70"/>
    <dbReference type="GeneID" id="6900999"/>
    <dbReference type="KEGG" id="dpo:6900999"/>
    <dbReference type="eggNOG" id="KOG1530">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_0_2_1"/>
    <dbReference type="InParanoid" id="B5DP70"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245688">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DP70">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd01519">
        <property type="entry name" value="RHOD_HSP67B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002134695.2"/>
        </source>
    </evidence>
    <sequence length="139" mass="15905" checksum="84E00E0BDE004ED9" modified="2016-01-20" version="2">MYLSVRAGKSVPYRLAYQLNAIFRPLCNMATYEQVKDVPNHSEIYLIDVRQKEELQQTGIIPASLNIPLPELDKAFCMDSKEFKSRYGRDKPGKDSKIIFSCRSGKRVLEAEKIAKAQGFTNVVIYKGSWLEWAQKEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>B5DQN8</accession>
    <accession>A0A6I8V0G9</accession>
    <name>B5DQN8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Inositol oxygenase</fullName>
            <ecNumber evidence="4 14">1.13.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 14">Myo-inositol oxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Miox</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>myo-inositol + O2 = D-glucuronate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="EC" id="1.13.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13 14">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the myo-inositol oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.99.1" evidence="4 14"/>
    <dbReference type="RefSeq" id="XP_002134834.1">
        <property type="nucleotide sequence ID" value="XM_002134798.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DQN8"/>
    <dbReference type="SMR" id="B5DQN8"/>
    <dbReference type="FunCoup" id="B5DQN8">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DQN8"/>
    <dbReference type="GeneID" id="6900443"/>
    <dbReference type="KEGG" id="dpo:6900443"/>
    <dbReference type="eggNOG" id="KOG1573">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050259_1_0_1"/>
    <dbReference type="InParanoid" id="B5DQN8"/>
    <dbReference type="OMA" id="RYNTKYG"/>
    <dbReference type="UniPathway" id="UPA00111">
        <property type="reaction ID" value="UER00527"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245104">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DQN8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050113">
        <property type="term" value="F:inositol oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007828">
        <property type="entry name" value="Inositol_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588:SF0">
        <property type="entry name" value="INOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588">
        <property type="entry name" value="MYOINOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05153">
        <property type="entry name" value="MIOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="7 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="227"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002134834.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="34274" checksum="E21B80B1D659A35C" modified="2008-10-14" version="1">MRILAENQIHLLDPSELMRPEPTFSDKNPSKFRDYSVDETDPLKERVRQTYRQMHLNQTVDFVKGRRNHWLKFNTIQMTVREALEKLNDLVDESDPDLDLPNIIHAFQAAERARAEFPQHDWLHLTALIHDLGKIMAFYGEPQWAVVGDTFAVGCRWGDSIVYRDESFVGNPDGANPAYNTEYGIYKPNCGVDNLLMSWGHDEYMYSVLKHNRTKLPDVACNIIRFHSFYPWHNGGDYKHLEAPKDEETKKWVLIFNRYDLYTKSEVVPDIEALWPYYQTLIDKYLPGVLEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-05-01" modified="2025-06-18" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>P25727</accession>
    <accession>B5DQP1</accession>
    <accession>O16174</accession>
    <accession>O16175</accession>
    <accession>O16176</accession>
    <accession>O16177</accession>
    <accession>O16178</accession>
    <accession>O16179</accession>
    <accession>O18663</accession>
    <name>EST5A_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Esterase-5A</fullName>
            <shortName>Est-5A</shortName>
            <ecNumber>3.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Carboxylic-ester hydrolase 5A</fullName>
            <shortName>Carboxylesterase-5A</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">Est-5A</name>
        <name type="synonym">Est5A</name>
        <name type="ORF">GA23705</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1990" name="Mol. Biol. Evol." volume="7" first="525" last="546">
            <title>Cloning of the esterase-5 locus from Drosophila pseudoobscura and comparison with its homologue in D. melanogaster.</title>
            <authorList>
                <person name="Brady J.P."/>
                <person name="Richmond R.C."/>
                <person name="Oakeshott J.G."/>
            </authorList>
            <dbReference type="PubMed" id="2178209"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>VARIANTS ILE-15; LEU-26 DEL; GLY-139; THR-226; VAL-228 AND VAL-269</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Genetics" volume="148" first="305" last="315">
            <title>The role of gene conversion in determining sequence variation and divergence in the Est-5 gene family in Drosophila pseudoobscura.</title>
            <authorList>
                <person name="King L.M."/>
            </authorList>
            <dbReference type="PubMed" id="9475741"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>VARIANTS ILE-15; LEU-26 DEL; GLY-139; THR-226; VAL-228; LEU-260; ASN-266; LEU-269; VAL-269; SER-281; THR-307; MET-327; LYS-330; THR-355; PHE-442; ASP-451; HIS-456; SER-487 AND PHE-546</scope>
        <source>
            <strain>GB115E</strain>
            <strain>GB139E</strain>
            <strain>GB336E</strain>
            <strain>GB4E</strain>
            <strain>GB8E</strain>
            <strain>JR198E</strain>
            <strain>JR341E</strain>
            <strain>JR50E</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a carboxylic ester + H2O = an alcohol + a carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:33308"/>
            <dbReference type="EC" id="3.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="sequence caution" evidence="6">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EDY73464" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.1.1.1"/>
    <dbReference type="EMBL" id="M55908">
        <property type="protein sequence ID" value="AAA28516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016135">
        <property type="protein sequence ID" value="AAB70225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016136">
        <property type="protein sequence ID" value="AAB70226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016137">
        <property type="protein sequence ID" value="AAB70227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016138">
        <property type="protein sequence ID" value="AAB70228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016139">
        <property type="protein sequence ID" value="AAB70229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016140">
        <property type="protein sequence ID" value="AAB70230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016141">
        <property type="protein sequence ID" value="AAB70231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016142">
        <property type="protein sequence ID" value="AAB70232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CH379069">
        <property type="protein sequence ID" value="EDY73464.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P25727"/>
    <dbReference type="SMR" id="P25727"/>
    <dbReference type="FunCoup" id="P25727">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="46245.P25727"/>
    <dbReference type="ESTHER" id="drops-est5a">
        <property type="family name" value="Carb_B_Arthropoda"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.947"/>
    <dbReference type="GlyCosmos" id="P25727">
        <property type="glycosylation" value="3 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1516">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="P25727"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00312">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000378">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0719">Serine esterase</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000008554" description="Esterase-5A">
        <location>
            <begin position="22"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="479"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="518"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB4E, GB8E, GB139E and GB336E." evidence="4 5">
        <original>V</original>
        <variation>I</variation>
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB4E, GB8E, GB139E and GB336E." evidence="4 5">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB4E, GB8E, GB115E, GB139E, GB336E, JR198E and JR341E." evidence="4 5">
        <original>S</original>
        <variation>G</variation>
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB4E, GB8E, GB139E, JR50E and JR198E." evidence="4 5">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB4E, GB8E, GB115E, GB139E, GB336E, JR50E and JR198E." evidence="4 5">
        <original>L</original>
        <variation>V</variation>
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB336E." evidence="5">
        <original>V</original>
        <variation>L</variation>
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR341E." evidence="5">
        <original>S</original>
        <variation>N</variation>
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB336E." evidence="5">
        <original>G</original>
        <variation>L</variation>
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB4E, GB8E, GB115E, GB139E, JR198E and JR341E." evidence="4 5">
        <original>G</original>
        <variation>V</variation>
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR341E." evidence="5">
        <original>I</original>
        <variation>S</variation>
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB115E." evidence="5">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR198E." evidence="5">
        <original>K</original>
        <variation>M</variation>
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR198E." evidence="5">
        <original>Q</original>
        <variation>K</variation>
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB139E." evidence="5">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR341E." evidence="5">
        <original>Y</original>
        <variation>F</variation>
        <location>
            <position position="442"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR341E." evidence="5">
        <original>E</original>
        <variation>D</variation>
        <location>
            <position position="451"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR198E." evidence="5">
        <original>Q</original>
        <variation>H</variation>
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB336E." evidence="5">
        <original>R</original>
        <variation>S</variation>
        <location>
            <position position="487"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB139E and JR50E." evidence="5">
        <original>C</original>
        <variation>F</variation>
        <location>
            <position position="546"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA28516." evidence="6" ref="1">
        <original>A</original>
        <variation>L</variation>
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA28516." evidence="6" ref="1">
        <original>GTVH</original>
        <variation>D</variation>
        <location>
            <begin position="467"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="2178209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="9475741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="548" mass="61376" checksum="AA16F36ED13D0B52" modified="2010-10-05" version="3" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q296F7</accession>
    <name>NMDA1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate [NMDA] receptor subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Nmdar1</name>
        <name type="ORF">GA15505</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. Plays a role in associative learning and in long-term memory consolidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heteromeric NMDA channel with Nmdar2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL28500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q296F7"/>
    <dbReference type="SMR" id="Q296F7"/>
    <dbReference type="FunCoup" id="Q296F7">
        <property type="interactions" value="395"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q296F7"/>
    <dbReference type="GlyCosmos" id="Q296F7">
        <property type="glycosylation" value="8 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0285978">
        <property type="protein sequence ID" value="FBpp0284416"/>
        <property type="gene ID" value="FBgn0075522"/>
    </dbReference>
    <dbReference type="GeneID" id="4802433"/>
    <dbReference type="KEGG" id="dpo:4802433"/>
    <dbReference type="CTD" id="40665"/>
    <dbReference type="eggNOG" id="KOG4440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007257_2_0_1"/>
    <dbReference type="InParanoid" id="Q296F7"/>
    <dbReference type="OMA" id="FANNTPD"/>
    <dbReference type="PhylomeDB" id="Q296F7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075522">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017146">
        <property type="term" value="C:NMDA selective glutamate receptor complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004970">
        <property type="term" value="F:glutamate-gated receptor activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035235">
        <property type="term" value="P:ionotropic glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007616">
        <property type="term" value="P:long-term memory"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008355">
        <property type="term" value="P:olfactory learning"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd06379">
        <property type="entry name" value="PBP1_iGluR_NMDA_NR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13719">
        <property type="entry name" value="PBP2_iGluR_NMDA_Nr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000177">
        <property type="entry name" value="Glutamate [NMDA] receptor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000266">
        <property type="entry name" value="Glutamate [NMDA] receptor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000010">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000025">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018882">
        <property type="entry name" value="CaM-bd_C0_NMDA_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049872">
        <property type="entry name" value="NMDA1-like_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049873">
        <property type="entry name" value="NMDA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10562">
        <property type="entry name" value="CaM_bdg_C0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0106">Calcium</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword id="KW-0675">Receptor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0770">Synapse</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000363999" description="Glutamate [NMDA] receptor subunit 1" evidence="5">
        <location>
            <begin position="40"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="5">
        <location>
            <begin position="40"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="5">
        <location>
            <begin position="607"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="664"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="5">
        <location>
            <begin position="685"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="844"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="5">
        <location>
            <begin position="865"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="980"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="994"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="542"/>
            <end position="544"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="549"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="715"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="759"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="5">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="5">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="5">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="5">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="5">
        <location>
            <position position="466"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="5">
        <location>
            <position position="493"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="5">
        <location>
            <position position="513"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="5">
        <location>
            <position position="705"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="3">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P35439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q05586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="4">
        <source>
            <dbReference type="UniProtKB" id="Q24418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5"/>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="EMBL" id="EAL28500.1"/>
        </source>
    </evidence>
    <sequence length="1004" mass="112855" checksum="C07DDC5E120A0497" modified="2006-04-04" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DK4</accession>
    <accession>A0A6I8UBM6</accession>
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        <submittedName>
            <fullName evidence="8">COP9 signalosome complex subunit 1b</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="primary">CSN1b</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN1 family.</text>
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        <property type="nucleotide sequence ID" value="XM_001352873.4"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CSN1_C"/>
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    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
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    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
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        <property type="entry name" value="Rpn7_N"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
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    </dbReference>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008793"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352909.1"/>
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    <sequence length="520" mass="57974" checksum="7416DA8355D839F0" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q29F32</accession>
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        <submittedName>
            <fullName evidence="10">Fork head domain-containing protein crocodile</fullName>
        </submittedName>
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        <name evidence="10" type="primary">croc</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
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        </source>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_001354152.4"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="Expressed in male reproductive system"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
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    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF388">
        <property type="entry name" value="FORK HEAD DOMAIN-CONTAINING PROTEIN L1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
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    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="DNA-binding region" description="Fork-head" evidence="7">
        <location>
            <begin position="70"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="336"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="379"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001354188.2"/>
        </source>
    </evidence>
    <sequence length="523" mass="56322" checksum="988EA4476130EB75" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FL2</accession>
    <accession>A0A6I8UEY3</accession>
    <name>Q29FL2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA-directed RNA polymerase III subunit rpc9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Polr3I</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues.</text>
    </comment>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. With POLR3H/RPC8 forms a mobile stalk that protrudes from Pol III core and functions primarily in transcription initiation. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the RNA polymerase III complex consisting of 17 subunits: a ten-subunit horseshoe-shaped catalytic core composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and functioning primarily in transcription initiation; and additional subunits homologous to general transcription factors of the RNA polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer required for transcription initiation and POLR3D/RPC4-POLR3E/RPC5 heterodimer involved in both transcription initiation and termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354335.2">
        <property type="nucleotide sequence ID" value="XM_001354299.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FL2"/>
    <dbReference type="FunCoup" id="Q29FL2">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FL2"/>
    <dbReference type="GeneID" id="4814215"/>
    <dbReference type="KEGG" id="dpo:4814215"/>
    <dbReference type="eggNOG" id="KOG4168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092529_5_0_1"/>
    <dbReference type="InParanoid" id="Q29FL2"/>
    <dbReference type="OMA" id="WGMHNLA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078496">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FL2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.40:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 17" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="15">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="121"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001354335.2"/>
        </source>
    </evidence>
    <sequence length="166" mass="19075" checksum="5F93A7F067A735F0" modified="2008-10-14" version="2">METVNATYSYLTNMEVMQVLQKIKSTKKKFGMRNLATVTYEALQYLEESPCKTQTRESITNYVKDLASYRLKSHEVLQMVNDPPTSPLHTQLLIDDNKAPLTDEENEKIIQLTYKHFYGVETTPQLPQQSQTQTQSSSKEKKTEQKQPASAPADGQTEQQQEQQKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-12-04" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q29H55</accession>
    <name>MB21L_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Protein mab-21-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA18415</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mab-21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">It is uncertain whether Met-1 or Met-7 is the initiator.</text>
    </comment>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EAL31902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29H55"/>
    <dbReference type="SMR" id="Q29H55"/>
    <dbReference type="FunCoup" id="Q29H55">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29H55"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0273760">
        <property type="protein sequence ID" value="FBpp0272198"/>
        <property type="gene ID" value="FBgn0078417"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:5614322"/>
    <dbReference type="eggNOG" id="KOG3963">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045315_0_0_1"/>
    <dbReference type="InParanoid" id="Q29H55"/>
    <dbReference type="OMA" id="WDESCIA"/>
    <dbReference type="PhylomeDB" id="Q29H55"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078417">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29H55">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000002">
        <property type="entry name" value="protein mab-21-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.90:FF:000001">
        <property type="entry name" value="protein mab-21-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF70">
        <property type="entry name" value="PROTEIN MAB-21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000312800" description="Protein mab-21-like">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="368" mass="42451" checksum="18268C1041FAB25B" modified="2006-04-04" version="1">MLVPPDMLVAQTRMIYQMNRYCAERVQARMFKTSTAIREICKIVQDILKEVELQEPRFISSLVECNGRFEGVEVISPNEFEIVLYLNQMGVFNFVDDGTLPGCAVLKLSDGRKRSMSLWVEFITASGYLSSRKIRSRFQTLVAQACDKSMYRDMVKIIGDTTEVKLRIRERYVVQITPAFKCSGIWPRSAAHWPMPHIPWPHPNIVAEVKTEGFDLLSKDSAAMQNKNNNAASMEGDAWVLSFYEAENRLLQGGCRRRCLSMLKTLRDRHLELPGSPISAYHLKNLLLYECEKHPRDYEWDESCIADRINGIFLQLISCLQYRRCPHYFLPSLDMFKGKSPSALEQAAKQVWRLTREMLTNANAFEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HI8</accession>
    <accession>A0A6I8UFJ5</accession>
    <name>Q29HI8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Splicing factor ESS-2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Es2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESS2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354715.2">
        <property type="nucleotide sequence ID" value="XM_001354679.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HI8"/>
    <dbReference type="FunCoup" id="Q29HI8">
        <property type="interactions" value="1393"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HI8"/>
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    <dbReference type="eggNOG" id="KOG2627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024820_0_1_1"/>
    <dbReference type="InParanoid" id="Q29HI8"/>
    <dbReference type="OMA" id="AQNDYLD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073249">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HI8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019148">
        <property type="entry name" value="Nuclear_protein_DGCR14_ESS-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940">
        <property type="entry name" value="ES-2 PROTEIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940:SF0">
        <property type="entry name" value="SPLICING FACTOR ESS-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09751">
        <property type="entry name" value="Es2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="102"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="276"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001354715.2"/>
        </source>
    </evidence>
    <sequence length="491" mass="55150" checksum="50E2EC4736E7CBDA" modified="2008-10-14" version="2">MSETPRTPGTPGSLAMEMVKAQNTALADFKKPTDLVRRKNKPKILTEEQYIEEMSKIIQRDFFPDLEKLRAQNDYLEAEARRDFLQMAEIRARYSLGRIPRMGCRNGRRNNRNNNAMSPATFETPVSQGSRSNTPLQRADTPCSTTSSSARSSTEGDSLPSKMSLDAFLQSYTSEDNQSFQEIIETAEEKLRQKYALLYNHEQLSADQLKRALMLPSIEKQFEEPDPLRKIETWKYTNMNSVMYVPDGVELTEEERVQAAERRMSIKHEATRLPDSLNNKHQEVENKKATDPAVGENDPTATPRIRGFELLRSPSPHPGVAFSPIMTWGEIDGTPFRLDGGDTPLRPTQGPSFRINENSRRENIAIALAERVSEKMRNQKQVAFDTARRNIGSPLIRTNMERLASMSPAAQHLATAKLGLRCTPSLSHTPTPGAARKRKITPGLVKSTTPLGKPRRITTPSRNTIIDTGSTITDDLLKIPIKPRSAAADFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q29K08</accession>
    <accession>A0A6I8UI33</accession>
    <name>Q29K08_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nuclear transcription factor Y subunit B-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Nf-YB</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355745.2">
        <property type="nucleotide sequence ID" value="XM_001355709.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29K08"/>
    <dbReference type="SMR" id="Q29K08"/>
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        <property type="interactions" value="1896"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29K08"/>
    <dbReference type="GeneID" id="4816206"/>
    <dbReference type="KEGG" id="dpo:4816206"/>
    <dbReference type="eggNOG" id="KOG0869">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066247_12_0_1"/>
    <dbReference type="InParanoid" id="Q29K08"/>
    <dbReference type="OMA" id="MQTDDAN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070381">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29K08">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000099">
        <property type="entry name" value="nuclear transcription factor Y subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="6">
        <location>
            <begin position="42"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001355745.2"/>
        </source>
    </evidence>
    <sequence length="156" mass="17633" checksum="7F8436FA84EAF757" modified="2008-10-14" version="2">MSNSEDSQQYITDMMVKEEDEASGDESDKQDNGIMLREQDRFLPICNIIKIMKVPVPQNGKIAKDARECIQECVSEFISFISSEAIERSVAENRKTVNGDDLIAAFGNLGFDNYVEPLQIYLNKYRESNKADRNLFMDTNFAQNEDGSTTNEGAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KP9</accession>
    <accession>A0A6I8UIU0</accession>
    <name>Q29KP9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4816563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356066.1">
        <property type="nucleotide sequence ID" value="XM_001356030.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KP9"/>
    <dbReference type="FunCoup" id="Q29KP9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KP9"/>
    <dbReference type="GeneID" id="4816563"/>
    <dbReference type="KEGG" id="dpo:4816563"/>
    <dbReference type="eggNOG" id="ENOG502S5Q4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153430_0_0_1"/>
    <dbReference type="InParanoid" id="Q29KP9"/>
    <dbReference type="OMA" id="QNTHLIP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074365">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KP9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356066.1"/>
        </source>
    </evidence>
    <sequence length="98" mass="10673" checksum="0B900EC64D5E01CE" modified="2006-04-04" version="1">MKVFALCSMLALLLAGAQALPQGREGAAYTNEAIRQAQQTLLIPKDAQIQNVQEGIELGAYEQIPGNQRINLFEILGDQVPSEVINNLQAQVDQIGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q29L99</accession>
    <accession>A0A6I8UL17</accession>
    <name>Q29L99_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polyphosphoinositide phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FIG4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357080.1">
        <property type="nucleotide sequence ID" value="XM_001357044.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29L99"/>
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        <property type="interactions" value="1341"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29L99"/>
    <dbReference type="GeneID" id="4817700"/>
    <dbReference type="KEGG" id="dpo:4817700"/>
    <dbReference type="eggNOG" id="KOG1888">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_0_3_1"/>
    <dbReference type="InParanoid" id="Q29L99"/>
    <dbReference type="OMA" id="KRKCCAH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074721">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29L99">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="675"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="688"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="707"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001357080.1"/>
        </source>
    </evidence>
    <sequence length="858" mass="98452" checksum="B2043CDDAEF8DBE6" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MU0</accession>
    <accession>A0A6I8UJI7</accession>
    <name>Q29MU0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">U6 snRNA-associated Sm-like protein LSm7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LSm7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome. Interacts with TACC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356539.1">
        <property type="nucleotide sequence ID" value="XM_001356503.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MU0"/>
    <dbReference type="FunCoup" id="Q29MU0">
        <property type="interactions" value="1696"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MU0"/>
    <dbReference type="GeneID" id="4817392"/>
    <dbReference type="KEGG" id="dpo:4817392"/>
    <dbReference type="eggNOG" id="KOG1781">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_3_1_1"/>
    <dbReference type="InParanoid" id="Q29MU0"/>
    <dbReference type="OMA" id="PFVQQEE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072212">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MU0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01729">
        <property type="entry name" value="LSm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000025">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017132">
        <property type="entry name" value="Lsm7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037188">
        <property type="entry name" value="U6_snRNA_Lsm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="19"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001356539.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="12092" checksum="4538CF7E098221DD" modified="2006-04-04" version="1">MADKKVGGNNDGKEKRRKESILDLSKYLEKQIRVKFAGGREASGILKGYDALLNLVLDNTVEYLRDSDEPYKLTEDSTRSLGLVVCRGTALVLICPADGVESIANPFTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NJ0</accession>
    <accession>A0A6I8UKY0</accession>
    <name>Q29NJ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Opsin Rh5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Rh5</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Membrane</location>
            <topology evidence="2 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357162.1">
        <property type="nucleotide sequence ID" value="XM_001357126.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NJ0"/>
    <dbReference type="SMR" id="Q29NJ0"/>
    <dbReference type="FunCoup" id="Q29NJ0">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NJ0"/>
    <dbReference type="GeneID" id="4817766"/>
    <dbReference type="KEGG" id="dpo:4817766"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009579_3_0_1"/>
    <dbReference type="InParanoid" id="Q29NJ0"/>
    <dbReference type="OMA" id="MLACKSV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078781">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29NJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008020">
        <property type="term" value="F:G protein-coupled photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15079">
        <property type="entry name" value="7tmA_photoreceptors_insect"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000044">
        <property type="entry name" value="Opsin, ultraviolet-sensitive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00577">
        <property type="entry name" value="OPSINRH3RH4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0157">Chromophore</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 16" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="12 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4 16" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="14 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="71"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="359"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001357162.1"/>
        </source>
    </evidence>
    <sequence length="384" mass="42716" checksum="779FF6F94FE67384" modified="2006-04-04" version="1">MHLNGPSGPQAFVNDSSGDVGGTLYPIGHSYPAEYQHMVHAHWRGFREAPIYYHAGIYIAFIVLMFSNLFGNGLVIWIFSTSKSLRTPSNLLILNLAVFDLFMCSNMPHYLLNAAVGYIVGGDLGCDIYALNGGISGMGASITNAFIAFDRYKTISNPIDGRLSYGQIVILIIFTWLWATPFSVLPLFQIWGRYQPEGFLTSCTFDYLSSTDENRLFVRTIFVWSYVIPMTMILTSYYKLFTHVRTHEKMLANQAKKMNVKSLSANASNDNMSVELRIAKAALIIYMLFILAWTPYSVVALIGCFGEQQLITPFVSMLPCLAAKSVSCLDPWVYATSHPKFRLELERRLPWLGIREKHAASGSSGGQESVASVSGDTLALSVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PH2</accession>
    <accession>A0A6I8ULA5</accession>
    <name>Q29PH2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">uncharacterized protein LOC4817912</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817912</name>
        <name evidence="1" type="synonym">Dpse\GA28678</name>
        <name evidence="1" type="ORF">Dpse_GA28678</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
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                <person name="Couronne O."/>
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                <person name="Worley K.C."/>
                <person name="Havlak P."/>
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                <person name="Egan A."/>
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                <person name="Dubchak I."/>
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            </authorList>
            <dbReference type="PubMed" id="15632085"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="1" key="2">
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                <consortium name="Drosophila 12 Genomes Consortium"/>
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                <person name="Begun D."/>
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                <person name="Bosak S.A."/>
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                <person name="Chapple C."/>
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                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
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                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
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                <person name="Lin C.F."/>
                <person name="Lin M.F."/>
                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
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                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
                <person name="O'Grady P."/>
                <person name="Pachter L."/>
                <person name="Papaceit M."/>
                <person name="Parisi M.J."/>
                <person name="Parisi M."/>
                <person name="Parts L."/>
                <person name="Pedersen J.S."/>
                <person name="Pesole G."/>
                <person name="Phillippy A.M."/>
                <person name="Ponting C.P."/>
                <person name="Pop M."/>
                <person name="Porcelli D."/>
                <person name="Powell J.R."/>
                <person name="Prohaska S."/>
                <person name="Pruitt K."/>
                <person name="Puig M."/>
                <person name="Quesneville H."/>
                <person name="Ram K.R."/>
                <person name="Rand D."/>
                <person name="Rasmussen M.D."/>
                <person name="Reed L.K."/>
                <person name="Reenan R."/>
                <person name="Reily A."/>
                <person name="Remington K.A."/>
                <person name="Rieger T.T."/>
                <person name="Ritchie M.G."/>
                <person name="Robin C."/>
                <person name="Rogers Y.H."/>
                <person name="Rohde C."/>
                <person name="Rozas J."/>
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                <person name="Ruiz A."/>
                <person name="Russo S."/>
                <person name="Salzberg S.L."/>
                <person name="Sanchez-Gracia A."/>
                <person name="Saranga D.J."/>
                <person name="Sato H."/>
                <person name="Schaeffer S.W."/>
                <person name="Schatz M.C."/>
                <person name="Schlenke T."/>
                <person name="Schwartz R."/>
                <person name="Segarra C."/>
                <person name="Singh R.S."/>
                <person name="Sirot L."/>
                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
                <person name="Smith T.F."/>
                <person name="Spieth J."/>
                <person name="Stage D.E."/>
                <person name="Stark A."/>
                <person name="Stephan W."/>
                <person name="Strausberg R.L."/>
                <person name="Strempel S."/>
                <person name="Sturgill D."/>
                <person name="Sutton G."/>
                <person name="Sutton G.G."/>
                <person name="Tao W."/>
                <person name="Teichmann S."/>
                <person name="Tobari Y.N."/>
                <person name="Tomimura Y."/>
                <person name="Tsolas J.M."/>
                <person name="Valente V.L."/>
                <person name="Venter E."/>
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                <person name="Villasante A."/>
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                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
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                <person name="Dupes A."/>
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                <person name="Hurhula B."/>
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                <person name="Kamat A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CH674172">
        <property type="protein sequence ID" value="EDY71603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001357252.1">
        <property type="nucleotide sequence ID" value="XM_001357216.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PH2"/>
    <dbReference type="STRING" id="46245.Q29PH2"/>
    <dbReference type="GeneID" id="4817912"/>
    <dbReference type="KEGG" id="dpo:4817912"/>
    <dbReference type="eggNOG" id="ENOG502S9UF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1887873_0_0_1"/>
    <dbReference type="OMA" id="RRDCDHI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072100">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EDY71603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357252.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="15636" checksum="A042BD7A6CF226F1" modified="2006-04-04" version="1">MIIPEVDMLAVSKNSRVQRAASAGYVLPGLKLVDYGGLDRVDRYYNDCQDYRDYYRDPYNKIHKPERFRHYQGKCGIRLDKSLNDLMLPPVWRVERKPIVYPIEYGIQMDMDKSYRTLLTGTHSATTHTAFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LYZ3</accession>
    <accession>A0A6I8UE16</accession>
    <name>Q2LYZ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell death protein rpr</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpr</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353980.1">
        <property type="nucleotide sequence ID" value="XM_001353944.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LYZ3"/>
    <dbReference type="FunCoup" id="Q2LYZ3">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LYZ3"/>
    <dbReference type="GeneID" id="4813234"/>
    <dbReference type="KEGG" id="dpo:4813234"/>
    <dbReference type="eggNOG" id="ENOG502TCC9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2851998_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LYZ3"/>
    <dbReference type="OMA" id="RESQWRF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078112">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353980.1"/>
        </source>
    </evidence>
    <sequence length="72" mass="8217" checksum="10171A294FCD9216" modified="2006-02-21" version="1">MAVAFYIPDQATLLRKAAQQEQELLRLRDSQWRFLATVVLETLRQFMDSAASQCLPRSGGGRKCGKYRKPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZI3</accession>
    <accession>A0A6I8UDJ2</accession>
    <name>Q2LZI3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Cytochrome b-c1 complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Complex III subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">UQCR-Q</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12 13">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with UQCC6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the UQCRQ/QCR8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353790.1">
        <property type="nucleotide sequence ID" value="XM_001353754.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZI3"/>
    <dbReference type="FunCoup" id="Q2LZI3">
        <property type="interactions" value="538"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZI3"/>
    <dbReference type="GeneID" id="4813793"/>
    <dbReference type="KEGG" id="dpo:4813793"/>
    <dbReference type="eggNOG" id="KOG4116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156007_2_0_1"/>
    <dbReference type="InParanoid" id="Q2LZI3"/>
    <dbReference type="OMA" id="GVPNMFR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080448">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZI3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.210:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.210">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004205">
        <property type="entry name" value="Cyt_bc1_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036642">
        <property type="entry name" value="Cyt_bc1_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02939">
        <property type="entry name" value="UcrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81508">
        <property type="entry name" value="Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001353790.1"/>
        </source>
    </evidence>
    <sequence length="89" mass="10063" checksum="497FC8AEC47D062A" modified="2006-02-21" version="1">MRLSSILNGAHFGNLAKVHGIITYKLSPFEQRAFAGAISNGVPNMVRRFRSNVFIVAPPFIIGYLIYDLTERKHTALLRKNPADYENDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M071</accession>
    <name>Q2M071_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hsp90 co-chaperone Cdc37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Cdc37</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC37 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353552.1">
        <property type="nucleotide sequence ID" value="XM_001353516.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M071"/>
    <dbReference type="SMR" id="Q2M071"/>
    <dbReference type="FunCoup" id="Q2M071">
        <property type="interactions" value="2176"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M071"/>
    <dbReference type="GeneID" id="4813053"/>
    <dbReference type="KEGG" id="dpo:4813053"/>
    <dbReference type="eggNOG" id="KOG2260">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046495_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M071"/>
    <dbReference type="OMA" id="AEQCIII"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071396">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M071">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.610:FF:000001">
        <property type="entry name" value="Hsp90 co-chaperone Cdc37-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013873">
        <property type="entry name" value="Cdc37_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF4">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08564">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01069">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="10">
        <location>
            <begin position="114"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 C-terminal" evidence="9">
        <location>
            <begin position="282"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="347"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="40"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="245"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="347"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="363"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001353552.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="45164" checksum="D31A8B9D15933003" modified="2006-02-21" version="1">MVDYSKWKNIEISDDEDDTHPNIDTPSLFRWRHQARVERMEEMDKEKEAMKKKRQSIQARLLGVKERISKKDGDEAALKKELEKIENEGKELDRVENDMLKKEKKTPWNVDTISKPGFEKTVINKKAGRKPDENLSEEQREQRMKQFVKENEKLCKQYGMLRKYDDSKRFLQEHLQLVCDETANYLVIWSINLEMEEKHDLMAHVAHQCICMQYILELAKQLDVDPRACVSSFFSKIQSCLPEYRQQFESEIKGFKERIQKRAQEKIQEALAQAEEEERQERMGPGGLDPADVFESLPDELKACFESRDIELLQKTIAAMPVDVAKHHMKRCVDSGLWVPNAADMQQADEAEAAATADEGDASVEKTDDAKSESAKEEPIYTGVSTEDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0N8</accession>
    <accession>A0A6I8UCQ8</accession>
    <name>Q2M0N8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4813659</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353385.2">
        <property type="nucleotide sequence ID" value="XM_001353349.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0N8"/>
    <dbReference type="STRING" id="46245.Q2M0N8"/>
    <dbReference type="GeneID" id="4813659"/>
    <dbReference type="KEGG" id="dpo:4813659"/>
    <dbReference type="eggNOG" id="ENOG502TBDY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1333159_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M0N8"/>
    <dbReference type="OMA" id="VKFDLTC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073033">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0N8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353385.2"/>
        </source>
    </evidence>
    <sequence length="208" mass="23659" checksum="BAE43BAD2F07FE51" modified="2008-10-14" version="2">MHSTGQKSLLRNMASSPYGKEVLIGNWAERRYEIDEKSNAAVPGVRAPEGCEKYQTVSQRTYTNAPFTGIDTVQNYSDQRQEAFQNLRKNSTSSLSLVDHISLTRNFTTTTTLTYEVLPQRFLKKKQLDSGNCGPPQRQPELDMLQSFGNLTRTYNYKKLFKCEKLLAHLAGKAQTTYRSSYNRALKSEPIMEFGPDYDGVVKFDLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M1C8</accession>
    <accession>A0A6I8UC70</accession>
    <name>Q2M1C8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">T-cell immunomodulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4813388</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353145.3">
        <property type="nucleotide sequence ID" value="XM_001353109.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M1C8"/>
    <dbReference type="FunCoup" id="Q2M1C8">
        <property type="interactions" value="606"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M1C8"/>
    <dbReference type="GeneID" id="4813388"/>
    <dbReference type="KEGG" id="dpo:4813388"/>
    <dbReference type="eggNOG" id="KOG4550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020272_2_0_1"/>
    <dbReference type="InParanoid" id="Q2M1C8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080549">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M1C8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057089">
        <property type="entry name" value="C2_TIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024881">
        <property type="entry name" value="Tip"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412:SF0">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23122">
        <property type="entry name" value="C2_ITFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13517">
        <property type="entry name" value="FG-GAP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="T-cell immunomodulatory protein TIP C2" evidence="8">
        <location>
            <begin position="461"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353145.3"/>
        </source>
    </evidence>
    <sequence length="607" mass="68392" checksum="55E0EDDDDF88E0BA" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P7V6</accession>
    <accession>A0A6I8VJZ7</accession>
    <name>A0A0R3P7V6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine-rich cell wall structural protein 1.0 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6900536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042884.1">
        <property type="nucleotide sequence ID" value="XM_015187398.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P7V6"/>
    <dbReference type="STRING" id="46245.A0A0R3P7V6"/>
    <dbReference type="GeneID" id="6900536"/>
    <dbReference type="InParanoid" id="A0A0R3P7V6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244934">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39956">
        <property type="entry name" value="GH09530P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39956:SF1">
        <property type="entry name" value="GH09530P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043657707" evidence="2">
        <location>
            <begin position="23"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015042884.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="32718" checksum="5A87F83C081AC0D8" modified="2016-01-20" version="1">MKGIVSCCVLVTILVIAADVHAYRKFGRDCRDIACAPGERCIISRVPCNAPDELEENQCGQYPVCEEVEQKQMSAGVALERRKREADQEGVGYGMGGSGMGKGNVNGGGNNGGNGIGARGQNGNGMGGNQMGGGNGMNGMGGPNRMGGPNGIGGGNNGGGNGMNGLGGPNRMGGPNGMGGSNNGGGNGMNGMGGRGGYGNGMGNNGMGNNGMGPGGMGGPNRMGGQIGMGGGYNGGNNGGGNGMNGMGGRNGNGMGNNGMGNNGMGSGGMGGPNRMGGPIGMGGGNNQNGNGMGPGNWRGNYNGNTGGNDGQGYRRNGGTSNQSYTYTSTLATSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P864</accession>
    <accession>A0A6I8VJU1</accession>
    <name>A0A0R3P864_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcium channel flower</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">fwe</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="20">Homomultimer. Associates with the dally/ magu complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the calcium channel flower family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015042830.1">
        <property type="nucleotide sequence ID" value="XM_015187344.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P864"/>
    <dbReference type="SMR" id="A0A0R3P864"/>
    <dbReference type="FunCoup" id="A0A0R3P864">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="GeneID" id="4813142"/>
    <dbReference type="InParanoid" id="A0A0R3P864"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079391">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019365">
        <property type="entry name" value="TVP18/Ca-channel_flower"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314:SF2">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10233">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01077">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_015042830.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="20757" checksum="0B47F3F19DE34371" modified="2016-01-20" version="1">MSFAEKITGLLARPNQQDPVGGPEQPWYLKYGSRLLGIVAAFFAILFGLWNVLSIITLSVSCLVAGIIQMIAGFIVMVLEAPCCFVCIEQVNGIADKVDAKPMYFRAGLYCALAVPPIFMCFGLASLFGSGLIFATGAVYAMMALGKKASAEDMRAAAQQTGYGGNGTAASTTNDRAGIVNNAQPFSFTGAVGTDSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCC1</accession>
    <name>A0A6I8UCC1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Venom protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4813339</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353208.3">
        <property type="nucleotide sequence ID" value="XM_001353172.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCC1"/>
    <dbReference type="SMR" id="A0A6I8UCC1"/>
    <dbReference type="FunCoup" id="A0A6I8UCC1">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813339"/>
    <dbReference type="InParanoid" id="A0A6I8UCC1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000006">
        <property type="entry name" value="Serine proteinase stubble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7 11" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026142313" evidence="8">
        <location>
            <begin position="24"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="127"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353208.3"/>
        </source>
    </evidence>
    <sequence length="373" mass="40964" checksum="E3B81E2217D138CF" modified="2020-08-12" version="1">MMLHLRHWPLMLAIASCIGPASTTATSTSTTTSSLSSIPGKYQALGAAAQHQPKKLKIGAGAGAGAAVNASSLGAAAGGGGRKPVFRQNPIKNWLGSFNRNNSPAAQNQTSPTCSCRCGERNDESRIVGGTTAGVSEYPWMARLSYFNRFYCGGTLINDRYVLTAAHCVKGFMWFMIKVTFGEHDRCNDKERPETRFVLRAFSQKFSFSNFDNDIALLRLNDRVPITSFIRPICLPRVEQRNELFVGTRAIATGWGTLKEDGKPSCLLQEVEVPVLDNEECVAQTNYTQKMITKNMMCSGYPGVGGRDSCQGDSGGPLVRLRPDDKRFEQIGIVSWGNGCARPNYPGVYTRVTKYLDWIVENSRDGCFCDEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UD58</accession>
    <name>A0A6I8UD58_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cilia- and flagella-associated protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4813302</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353593.3">
        <property type="nucleotide sequence ID" value="XM_001353557.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UD58">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813302"/>
    <dbReference type="InParanoid" id="A0A6I8UD58"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14885:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14885">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 43-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25828">
        <property type="entry name" value="CC_Cfap43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="845"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="851"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353593.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1595" mass="185643" checksum="55CB4552BBFA8FE8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDN5</accession>
    <name>A0A6I8UDN5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Collagen alpha-2(I) chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4813329</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353741.3">
        <property type="nucleotide sequence ID" value="XM_001353705.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDN5"/>
    <dbReference type="FunCoup" id="A0A6I8UDN5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813329"/>
    <dbReference type="InParanoid" id="A0A6I8UDN5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026138341" evidence="2">
        <location>
            <begin position="44"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353741.3"/>
        </source>
    </evidence>
    <sequence length="340" mass="34888" checksum="5E58776D80D9E437" modified="2020-08-12" version="1">MNLIKARFQVIAQISLFRNDSMRSSVLLAILLLGICICSGVQASLVKCSCDPGPAGERGPPGPPGPPGAGFGSGGGGGGGIGGGHGGGFWPYPPTPPFTPNLPIIPGPRGLPGPPGPPGYCFPCPAVPPPIRAYPPPLGVPVPPFLSTQVGGGGGNAASALTSAVGNIIVIPSGAGTGLTGRAQFYHISPDGQVLRIDPPKDVPNELFDSLGASATNPAPQPPQPLQPTSASLSGITPPPTQATPGEQQQTTVTPEVLDETVFAVSGNRGKDFLRGNSEASDWRRILLLGERPTEAPIAQHHPSQLGNSMESFQRALVELKEKYATLMQPRSGNRYAVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFK6</accession>
    <name>A0A6I8UFK6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4815168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354727.3">
        <property type="nucleotide sequence ID" value="XM_001354691.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFK6"/>
    <dbReference type="KEGG" id="dpo:4815168"/>
    <dbReference type="InParanoid" id="A0A6I8UFK6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026065101" evidence="2">
        <location>
            <begin position="22"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354727.3"/>
        </source>
    </evidence>
    <sequence length="335" mass="35776" checksum="79E744B733B120F7" modified="2020-08-12" version="1">MEGILSLRFLILMAVVCVVVAQTATTKEPMRTTTGKPMKDIKEATDKRDKRQLGSNVGAAASTGGNFPIFYDGVNVNPPLQPLQPLQPLQPLQPLPPLQQLQPLPQLQPITVVQPGASSGNLQSQPQTGWLPNFGQNFGQNLPIIGTLVGGLPNLINSLGLGNLNGGFGTLGGINLGNLGNLGLGGLTPVVATPVPVPVPITGVSASNQQCPLTQKLSCRCEPLIQLPGLKPSSQLRGPQSQSQSQTQLVQILRQNVLRNDDGSQELRVILSSGHVVYQRTAKDLGQSGYFAMRIQTGRYFNIYYTVNDKEYTVRADVKDAPPSSDFDEGLSGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGR1</accession>
    <name>A0A6I8UGR1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Keratin-associated protein 19-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4816032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355227.2">
        <property type="nucleotide sequence ID" value="XM_001355191.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGR1"/>
    <dbReference type="KEGG" id="dpo:4816032"/>
    <dbReference type="InParanoid" id="A0A6I8UGR1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074687">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026039879" evidence="1">
        <location>
            <begin position="20"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355227.2"/>
        </source>
    </evidence>
    <sequence length="115" mass="12453" checksum="D3B96123AC21AB24" modified="2020-08-12" version="1">MKFLCVFVAFAICLSVSWAAISEPAAALPEDPEQDTAAIAEKKTEKRGIYSFGFDGYGHGYGGYGGYGGYGHGYGGYAHNSYGYGSPYGSFGYAPYYGGHHGFYPHHGHAHYSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UI94</accession>
    <name>A0A6I8UI94_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816399</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355909.3">
        <property type="nucleotide sequence ID" value="XM_001355873.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UI94"/>
    <dbReference type="KEGG" id="dpo:4816399"/>
    <dbReference type="InParanoid" id="A0A6I8UI94"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026361061" description="Secreted protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355909.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11153" checksum="88E46417AB343BEA" modified="2020-08-12" version="1">MQSILGLLLLCGAIYSVIAASGIHQYIGGGGADGGSDEKLQWSDSAHNADTDEILRRVAAFDGATNFDDSHWGGDAPSRGGGSDRASHSDSNSDADGPHRGFQFIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJ57</accession>
    <name>A0A6I8UJ57_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA polymerase I-specific transcription initiation factor RRN3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Tif-IA</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRN3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356293.3">
        <property type="nucleotide sequence ID" value="XM_001356257.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJ57"/>
    <dbReference type="FunCoup" id="A0A6I8UJ57">
        <property type="interactions" value="1817"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816431"/>
    <dbReference type="InParanoid" id="A0A6I8UJ57"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJ57">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007991">
        <property type="entry name" value="RNA_pol_I_trans_ini_fac_RRN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790:SF0">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IA RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05327">
        <property type="entry name" value="RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356293.3"/>
        </source>
    </evidence>
    <sequence length="609" mass="69444" checksum="D903083F2ED4243F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJB9</accession>
    <name>A0A6I8UJB9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">Polr1B</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">MV-25-SWS-2005</strain>
            <tissue evidence="23">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="RefSeq" id="XP_001356442.3">
        <property type="nucleotide sequence ID" value="XM_001356406.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJB9"/>
    <dbReference type="FunCoup" id="A0A6I8UJB9">
        <property type="interactions" value="1126"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816875"/>
    <dbReference type="InParanoid" id="A0A6I8UJB9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000016">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009674">
        <property type="entry name" value="Rpa2_dom_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06883">
        <property type="entry name" value="RNA_pol_Rpa2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 23" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="32"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="182"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="453"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase I subunit RPA2" evidence="21">
        <location>
            <begin position="561"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="666"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1021"/>
            <end position="1122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF06883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_001356442.3"/>
        </source>
    </evidence>
    <sequence length="1126" mass="127881" checksum="A1457D3B39C232FE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJQ7</accession>
    <name>A0A6I8UJQ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tripartite motif-containing protein 45</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4817149</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356614.3">
        <property type="nucleotide sequence ID" value="XM_001356578.4"/>
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    <dbReference type="KEGG" id="dpo:4817149"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF291">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-B_box"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="487"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="555"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="766"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356614.3"/>
        </source>
    </evidence>
    <sequence length="943" mass="104234" checksum="CB413A6067BD7BC6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM13</accession>
    <name>A0A6I8UM13_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">28S ribosomal protein S18c, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mRpS18C</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
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    <dbReference type="RefSeq" id="XP_001357678.4">
        <property type="nucleotide sequence ID" value="XM_001357641.5"/>
    </dbReference>
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        <property type="interactions" value="893"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800393"/>
    <dbReference type="InParanoid" id="A0A6I8UM13"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.10:FF:000018">
        <property type="entry name" value="28S ribosomal protein S18C, mitochondrial"/>
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    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF40">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS18M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357678.4"/>
        </source>
    </evidence>
    <sequence length="146" mass="16752" checksum="FE9FD14E43008497" modified="2020-08-12" version="1">MLKITKFIAQNATVVNLAGSRLARPASRFYAQTGSAEGKTTTRDSDEPIDITNPFEKDPQQCILCKNNITPHYKNVRLLSQFQSSYTGRIYGRHITGLCKQKQEDVERAILRAQQCLLMPGYHKDLDFLQDPKLFDPERPVRPHKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM81</accession>
    <name>A0A6I8UM81_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extensin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4800503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357769.4">
        <property type="nucleotide sequence ID" value="XM_001357732.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM81"/>
    <dbReference type="FunCoup" id="A0A6I8UM81">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800503"/>
    <dbReference type="InParanoid" id="A0A6I8UM81"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357769.4"/>
        </source>
    </evidence>
    <sequence length="319" mass="35287" checksum="8232EAEC6E3F0D09" modified="2020-08-12" version="1">MRYLLGFSGASLPDTFSECTACIQNPRKRPTTNMQMEKRTLLILLLGLVLCLESSQAARRLRKKPKVYNALITTDDNLTSSRAYPVIQPTIHEPGYAPFGPYNPYGFYSPPLVRFGQPIVPGLTPNERLPYPLPTGAQYPAGFSQYEPQQPVETPVEQQPLPQAAPPSLEPKEMTKEQMPLPLNEQGLPPVLIRLPSQYTGQPTHLPPYPYSQYPVIYDQAGYVRQNYLPPYDYYPTQGYPIKEPTAASAANAPAAPPPPSPPQEEPQALPLDLSLEPVKPSFEDIRNGSESKNSEVPDVPPPPIPSGPRRPRPAEGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMK9</accession>
    <name>A0A6I8UMK9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxysteroid dehydrogenase-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Hsdl2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357896.1">
        <property type="nucleotide sequence ID" value="XM_001357859.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMK9"/>
    <dbReference type="FunCoup" id="A0A6I8UMK9">
        <property type="interactions" value="1108"/>
    </dbReference>
    <dbReference type="GeneID" id="4800664"/>
    <dbReference type="KEGG" id="dpo:4800664"/>
    <dbReference type="InParanoid" id="A0A6I8UMK9"/>
    <dbReference type="OMA" id="STNFFDM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078990">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09762">
        <property type="entry name" value="HSDL2_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000301">
        <property type="entry name" value="Hydroxysteroid dehydrogenase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051935">
        <property type="entry name" value="HSDL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006133">
        <property type="entry name" value="PRK08278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808:SF3">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="9">
        <location>
            <begin position="316"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357896.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="44297" checksum="68697A3147B8D527" modified="2020-08-12" version="1">MINSGKLAGRTLFITGASRGIGKEIALKAARDGANIVVAAKTAEPHPKLPGTIYSAAEEIEKAGGRAHPCIVDVRDEVQVRTAIQEAVAKFGGIDIVVNNASAISLTNTPNTDMKRYDLMHNINTRGTFLVSKECLPYLKKSNHAHILNISPPLSMLPKWFGPHVAYTMAKYGMSMCVLGMAEEFKNDGIAVNALWPRTAILTAAIEMLTGSESAQWSRKPEIMADAAYAILSREPKQFTGQFFVDDEVLEAAGIKDLKPYACVPENADNLMVDFFVEAKGATTETLAATAGASTPTAGSDSGKIPQLFQKIESLLSTEIVSKTQAVFQFNISGAEQGTWYLDLKNGSGSCGSGTPPATPDATLTMNSTNFFDMFSGKLKAAPAYMTGKLKISGDFQKALKLEKLMKALKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMR1</accession>
    <name>A0A6I8UMR1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Attractin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">dsd</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357888.3">
        <property type="nucleotide sequence ID" value="XM_001357851.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UMR1">
        <property type="interactions" value="1300"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800655"/>
    <dbReference type="InParanoid" id="A0A6I8UMR1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000079">
        <property type="entry name" value="Attractin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000141">
        <property type="entry name" value="Attractin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000046">
        <property type="entry name" value="Attractin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000147">
        <property type="entry name" value="Distracted, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056732">
        <property type="entry name" value="GBD_ATRN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051568">
        <property type="entry name" value="LZTR1/Attractin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376:SF2">
        <property type="entry name" value="DISTRACTED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46376">
        <property type="entry name" value="LEUCINE-ZIPPER-LIKE TRANSCRIPTIONAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24972">
        <property type="entry name" value="GBD_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="11 13" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1227"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="16">
        <location>
            <begin position="140"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="303"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="18">
        <location>
            <begin position="1009"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1354"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="14"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1359"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1027"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1039"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001357888.3"/>
        </source>
    </evidence>
    <sequence length="1382" mass="153488" checksum="9F6959E31AB77138" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNG8</accession>
    <name>A0A6I8UNG8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">bb8</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 11">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358343.4">
        <property type="nucleotide sequence ID" value="XM_001358306.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNG8"/>
    <dbReference type="FunCoup" id="A0A6I8UNG8">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801220"/>
    <dbReference type="InParanoid" id="A0A6I8UNG8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF7">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="248"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="10">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001358343.4"/>
        </source>
    </evidence>
    <sequence length="539" mass="59856" checksum="CC39DD33B4A536D6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNT9</accession>
    <name>A0A6I8UNT9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenosine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Adgf-D</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenosine + H2O + H(+) = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. ADGF subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001358417.3">
        <property type="nucleotide sequence ID" value="XM_001358380.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNT9"/>
    <dbReference type="FunCoup" id="A0A6I8UNT9">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801298"/>
    <dbReference type="InParanoid" id="A0A6I8UNT9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01321">
        <property type="entry name" value="ADGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000017">
        <property type="entry name" value="Adenosine deaminase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013659">
        <property type="entry name" value="A_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006331">
        <property type="entry name" value="ADGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01431">
        <property type="entry name" value="adm_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF39">
        <property type="entry name" value="ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08451">
        <property type="entry name" value="A_deaminase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026101381" description="Adenosine deaminase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Adenosine/AMP deaminase N-terminal" evidence="13">
        <location>
            <begin position="5"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Adenosine deaminase" evidence="12">
        <location>
            <begin position="190"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358417.3"/>
        </source>
    </evidence>
    <sequence length="499" mass="57794" checksum="2AC44E2BB097BCD6" modified="2020-08-12" version="1">MLLGLLLCLVPPLLGGLHAADLPYETLREQIMEAERLASLGGNIWLTSDEEKANSILMNAKRAEIAEGIKTPEKYPPAMHFFQGRQYVRQSEVFRFIQKMPKGAFLHGHNTGMVTSKWVIGNLTTTNNLYTCRNVDGLLIFTYDQSGCHSEVQNVCTERINAEDRGKYERQLEKHINMHRQKPEALLSQRKKIWERFENIFTTVDRLYKYRPSYCAFHKRLLEELCEDNIIYAEVRASLSPLYDDNNRTLSTLEVANELERIVEEFKAKHHDFVGLKVIYAKRNRATEEEMSRRITTFKQLHHAKPNFVIGFDLIGQEDTGDPLNKYVNELSDLPSTANYFFHAGETNWHGRTDWNMMDAILLDSKRIGHAFALPKHPQLWSTIKKRDIAIEVNPISNQVLGFVWDLRNHPASFLIAQNFPIVISSDDPGMWGAKGLSYDFYYAFMAMAPAEADLRFLKQLALNSIKYAVLTSDERRKINRIFQRKWREFIANVLNPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPA3</accession>
    <name>A0A6I8UPA3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">26S proteasome regulatory subunit 6A-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Rpt5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358632.1">
        <property type="nucleotide sequence ID" value="XM_001358595.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPA3"/>
    <dbReference type="SMR" id="A0A6I8UPA3"/>
    <dbReference type="FunCoup" id="A0A6I8UPA3">
        <property type="interactions" value="1939"/>
    </dbReference>
    <dbReference type="GeneID" id="4801560"/>
    <dbReference type="KEGG" id="dpo:4801560"/>
    <dbReference type="InParanoid" id="A0A6I8UPA3"/>
    <dbReference type="OMA" id="NKISHEH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070338">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
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        <property type="entry name" value="ATPase_AAA_core"/>
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        <property type="entry name" value="ATPase_AAA_CS"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
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        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="AAA"/>
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    </dbReference>
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    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="208"/>
            <end position="347"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00006914"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022942"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="RuleBase" id="RU003651"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358632.1"/>
        </source>
    </evidence>
    <sequence length="428" mass="47785" checksum="4BE2648B2B9527B6" modified="2020-08-12" version="1">MAQTLEDKSIWEDGEESLGEEVMRMSTDEIVSRTRLMDNEIKIMKSEVMRITHEIQAQNEKIKDNTEKIKVNKTLPYLVSNVIELLDVDPQEEEDDGSVTVLDNQRKGKCAVIKTSTRQAYFLPVIGLVDAEKLKPGDLVGVNKDSYLILETLPAEYDARVKAMEVDERPTEQYSDIGGLDKQIQELIEAVVLPMTHKEKFKNLGIHPPKGVLLYGPPGTGKTLLARACAAQTKSTFLKLAGPQLVQMFIGDGAKLVRDAFALAKEKAPAIIFIDELDAIGTKRFDSEKAGDREVQRTMLELLNQLDGFSSTADIKVIAATNRVDILDPALLRSGRLDRKIEFPHPNEEARARIMQIHSRKMNVSTDVNFEELSRSTDDFNGAQCKAVCVEAGMIALRRSASSVTHEDFMDAIMEVQAKKKANLNYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ03</accession>
    <name>A0A6I8UQ03_DROPS</name>
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        <submittedName>
            <fullName evidence="9">Histone PARylation factor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Hpf1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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    </comment>
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        <text evidence="3">Belongs to the HPF1 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001359021.5"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:poly-ADP-D-ribose binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019406">
        <property type="entry name" value="APLF_PBZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019361">
        <property type="entry name" value="HPF1"/>
    </dbReference>
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        <property type="entry name" value="HISTONE PARYLATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13386:SF1">
        <property type="entry name" value="HISTONE PARYLATION FACTOR 1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF10228">
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        <property type="entry name" value="zf-CCHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PBZ-type" evidence="7">
        <location>
            <begin position="3"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="43"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010803"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359058.2"/>
        </source>
    </evidence>
    <sequence length="442" mass="50358" checksum="BC8BA2A8E3E93FDE" modified="2020-08-12" version="1">MPKEDCKYGEKCYQRNAAHLAKYNHPDKTDDGQDKAEVVKLAPKRKEASCGEEEAVAPNTSNSSGESQNMEKSHGEAKGTFESETAELHREAMSNISGKNYMEILEKRIRLSVQQEYDTLCESNEFIRHKFLVEMPPDFYEFWKFINTLTQDSSKSNRAAVEHLETVFQLQLVGPFEFLSGAFHRATMGEPGDYLRHWRFYYDPPEFQTVFVRRGTGVHYGYWRDVPQDKENLLIARNDMTNGCEFQFVSGNIFDAFLYYLEHDFVGTPFTTGKVTATKKAVQKYLAENSLELAQLDRLQRERNKRVVAKAFHRGGIVVPFDRKTQLGYRPLSVSDAELKKMLALFERKDLGDGLAKQAVLEKLQPVANAAIIAVDECDFGSALELGIDLFSSGHKELHMLASSLIVPAYSMLQRPQFIAIAKAHMESRSRDLNLSMFDVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ57</accession>
    <name>A0A6I8UQ57_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DDB1- and CUL4-associated factor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4801938</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358955.5">
        <property type="nucleotide sequence ID" value="XM_001358918.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ57"/>
    <dbReference type="FunCoup" id="A0A6I8UQ57">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801938"/>
    <dbReference type="InParanoid" id="A0A6I8UQ57"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045717">
        <property type="term" value="P:negative regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045151">
        <property type="entry name" value="DCAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF43">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="329"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="423"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="590"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="637"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="674"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="696"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="725"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358955.5"/>
        </source>
    </evidence>
    <sequence length="738" mass="81992" checksum="E0D356586793CA98" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQW8</accession>
    <name>A0A6I8UQW8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359287.3">
        <property type="nucleotide sequence ID" value="XM_001359250.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQW8"/>
    <dbReference type="KEGG" id="dpo:4802358"/>
    <dbReference type="InParanoid" id="A0A6I8UQW8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF6">
        <property type="entry name" value="CHK DOMAIN OV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="138"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359287.3"/>
        </source>
    </evidence>
    <sequence length="422" mass="49323" checksum="4FBC8E4A8B9678DC" modified="2020-08-12" version="1">MEKIENPNESLVIPKWLNEDKFKVLLSKDEPNYNKILQFTPVAAIPPGSNFTSILARVYLDLELKDGSLRRQSYVVKTTLELDKGGRLVEEFRYFQKEQQMYSTYLPAFEQLYREAGHPIQLVPKCLEIGEMDGYLYFIFEDLSSQEFVTVDRSKGLDMEHMNMSLRKLAELHAASVVYEDRHGPYSIDFEHGFAHKDNTEHSRRGFDAKVTEYKAAMLTWGIEERCLENFPTPQQYIEMCLESLKTDANDFNVLTHGDFWSSNILFKYNSYNNLDKALLLDFQHCKWGSPAQDLLFIITISAGRELRLREYDNFVRIYWEHLIDCLAILSHKKLLPQLRDLNAALYKKNNTFYAFFPVMSHLSTVLLPTDKDSNLHTLMSQDDAGRSLRTRLYTNPAFVEVIKEVYPFYCNRGLFNFVDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US42</accession>
    <name>A0A6I8US42_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein peg</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">peg</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360069.2">
        <property type="nucleotide sequence ID" value="XM_001360032.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US42"/>
    <dbReference type="FunCoup" id="A0A6I8US42">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="GeneID" id="4803308"/>
    <dbReference type="KEGG" id="dpo:4803308"/>
    <dbReference type="InParanoid" id="A0A6I8US42"/>
    <dbReference type="OMA" id="CDTQEYR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074462">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026358878" evidence="1">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="2">
        <location>
            <begin position="29"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360069.2"/>
        </source>
    </evidence>
    <sequence length="80" mass="8814" checksum="4773059441F440E2" modified="2020-08-12" version="1">MKLFAVFLALALYALTVVQGLSLPDPNVKCAMDCDTQEYAAICAADDKGTTRTYRNLCVMKTENCLQNASFQKISDKECP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US43</accession>
    <name>A0A6I8US43_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4803132</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001359925.3">
        <property type="nucleotide sequence ID" value="XM_001359888.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US43"/>
    <dbReference type="FunCoup" id="A0A6I8US43">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803132"/>
    <dbReference type="InParanoid" id="A0A6I8US43"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026370989" description="UDP-glucuronosyltransferase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359925.3"/>
        </source>
    </evidence>
    <sequence length="524" mass="59624" checksum="5B49C6F2DC36F0F1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US98</accession>
    <name>A0A6I8US98_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">General transcription factor IIF subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Transcription initiation factor IIF subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TfIIFbeta</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359998.2">
        <property type="nucleotide sequence ID" value="XM_001359961.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US98"/>
    <dbReference type="SMR" id="A0A6I8US98"/>
    <dbReference type="FunCoup" id="A0A6I8US98">
        <property type="interactions" value="1879"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803222"/>
    <dbReference type="InParanoid" id="A0A6I8US98"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015849">
        <property type="entry name" value="TFIIF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="11">
        <location>
            <begin position="23"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="10">
        <location>
            <begin position="197"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359998.2"/>
        </source>
    </evidence>
    <sequence length="282" mass="32402" checksum="ACBBA245BBBD99D3" modified="2020-08-12" version="1">MSKDDKDKVQIIDKDLDLSNAGRGVWLVKVPKYIAQKWEKAPSNMDVGKLRISKTPGQKAQVSLSLTPAVLALDPDEKIPTEHVLDVSQVTKQTLGVFSHMAPEAAANIGKENSTAPGAAPDNEKLYMEGRIVQKLECRPIADTCYMKLKLESIRKASEPQRRVQPIDKIVQNFKPVKDHAHNIEYRERKKAEGKKARDDKNSVMDMLFHAFEKHQYYNIKDLVKITNQPISYLKEILKDVCDYNMKNPHKNMWELKKEYRHYKTEEKKDEEEHKSGSSDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USM4</accession>
    <name>A0A6I8USM4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isochorismatase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4803401</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360132.1">
        <property type="nucleotide sequence ID" value="XM_001360095.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USM4"/>
    <dbReference type="FunCoup" id="A0A6I8USM4">
        <property type="interactions" value="1098"/>
    </dbReference>
    <dbReference type="GeneID" id="4803401"/>
    <dbReference type="KEGG" id="dpo:4803401"/>
    <dbReference type="InParanoid" id="A0A6I8USM4"/>
    <dbReference type="OMA" id="QAGCVIT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077608">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd01012">
        <property type="entry name" value="YcaC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.850:FF:000001">
        <property type="entry name" value="Isochorismatase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050993">
        <property type="entry name" value="Isochorismatase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119:SF17">
        <property type="entry name" value="ISOCHORISMATASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="13"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360132.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="23215" checksum="959AB3B61B744C54" modified="2020-08-12" version="1">MALRRCHLNPKKTLFLLCDIQEKFRLAMPLFENMIKNVDKLTKAGKVLDVPLIVTEHYPEKLGKTVAQLDVSHAKLVSSKTLFSMVTPEVKAVIKDIFSGKPEDVVLYGLESHICVEQTAIDLLEQNINVYIVADCCSSRLNQDRDLALDRLQQAGCVITTSESVIFDLVRDKNNAQFDTIRKLVSQTSVDMELRRTGAGLPVGSAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USP4</accession>
    <name>A0A6I8USP4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger protein 277</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4803620</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZNF277 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360309.2">
        <property type="nucleotide sequence ID" value="XM_001360272.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USP4"/>
    <dbReference type="FunCoup" id="A0A6I8USP4">
        <property type="interactions" value="1709"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803620"/>
    <dbReference type="InParanoid" id="A0A6I8USP4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040048">
        <property type="entry name" value="ZNF277"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13267">
        <property type="entry name" value="ZINC FINGER PROTEIN 277"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13267:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN 277"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360309.2"/>
        </source>
    </evidence>
    <sequence length="447" mass="52496" checksum="B627F2F472129CB1" modified="2020-08-12" version="1">MEHAVDTVPEDVAPKLENKFSSVLKPTANTVVKCLKCEKVYIFPADKDDCLAHLYLEHRLVIADVEDIALLEDYLQYWQKEFHSHEFEQYCTTMFLDQLPDGTYARNEKYYLLCDILPQDYELRQRLREERLKTALERHHFELTDRNFSKECLFCRTVIKGLRADYLDHLFDKHFLLVGKPEKLVYVDELLDQLEANLNQLICLYCEKVFKDRTTLKEHMRKKGHKRINPNRREYDKYFLINYNRAPAPAPRKQQHQKRRQPSGSVSADVENMDFDKHFARPDSEGEHDSDWSDWAGDGEPHSLKCLYCPHFGDHFAGLKQHMIDAHSLDFDVATSALNFYQRVKVVNYIRRQLCMRRCVSCDIQFDDLDLLTEHMAQESHCGIGERSSWDKPEFFFPYMENDGLLCVLDDSTGDEPDTDVVRIISEDSLAQVNKDAESLSLENFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTF5</accession>
    <name>A0A6I8UTF5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein row</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">row</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360640.3">
        <property type="nucleotide sequence ID" value="XM_001360603.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTF5"/>
    <dbReference type="FunCoup" id="A0A6I8UTF5">
        <property type="interactions" value="536"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804009"/>
    <dbReference type="InParanoid" id="A0A6I8UTF5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057618">
        <property type="entry name" value="Znf_POGZ/Z280C-D-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF60">
        <property type="entry name" value="RELATIVE OF WOC, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25429">
        <property type="entry name" value="zf-POGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="906"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="159"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="595"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1087"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="642"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360640.3"/>
        </source>
    </evidence>
    <sequence length="1271" mass="141878" checksum="6EB59CA651E67577" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTH6</accession>
    <name>A0A6I8UTH6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Ntmt</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.244" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001360524.4">
        <property type="nucleotide sequence ID" value="XM_001360487.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTH6"/>
    <dbReference type="FunCoup" id="A0A6I8UTH6">
        <property type="interactions" value="1846"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803875"/>
    <dbReference type="InParanoid" id="A0A6I8UTH6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000025">
        <property type="entry name" value="N-terminal Xaa-Pro-Lys N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="163"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001360524.4"/>
        </source>
    </evidence>
    <sequence length="272" mass="30214" checksum="92E6AD5EF56A3A09" modified="2020-08-12" version="1">MPLTEGPAPEKCVNTLKDEMDSSEDKECTQEHRNGPEAKVADAVAISSAASNAQEPKFYGKAQKYWSGVPATVNGMLGGMGYISATDIQGSNNFLREIRVPGNKLALDCGAGIGRITRHLLIPRFHCVDLVEQDPAFANKAREYCTTENTPVGSLGEIYNVGLQKFTPTKKYDLIWSQWVLGHLTELDLIDFFRRIRQGLAPGAFFVLKENVTKSKEIIVDDEDSSVTRPLEHYDISLKAAGFRIVRKVRQQNFPKDLYPVYMIACKPLSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTX6</accession>
    <name>A0A6I8UTX6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein MTO1 homolog, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4804239</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360830.1">
        <property type="nucleotide sequence ID" value="XM_001360793.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTX6"/>
    <dbReference type="FunCoup" id="A0A6I8UTX6">
        <property type="interactions" value="1643"/>
    </dbReference>
    <dbReference type="GeneID" id="4804239"/>
    <dbReference type="KEGG" id="dpo:4804239"/>
    <dbReference type="InParanoid" id="A0A6I8UTX6"/>
    <dbReference type="OMA" id="CNPAMGG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078304">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000082">
        <property type="entry name" value="protein MTO1 homolog, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.570:FF:000001">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000002">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="entry name" value="GidA associated domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
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        <property type="entry name" value="GIDA_C_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047001">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
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        <property type="entry name" value="MnmG_C_subdom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00136">
        <property type="entry name" value="mnmG_gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="GLUCOSE INHIBITED DIVISION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GIDA_C_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="SAM_GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PNDRDTASEI"/>
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        <property type="entry name" value="GIDA_assoc_3"/>
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        <property type="entry name" value="GIDA_2"/>
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    <proteinExistence type="inferred from homology"/>
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    <feature type="domain" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain" evidence="5">
        <location>
            <begin position="577"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360830.1"/>
        </source>
    </evidence>
    <sequence length="659" mass="73574" checksum="3D41C1364CDB9139" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV51</accession>
    <name>A0A6I8UV51_DROPS</name>
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        <submittedName>
            <fullName evidence="9">LDLR chaperone boca</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">boca</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
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        <text evidence="2">Belongs to the MESD family.</text>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:protein folding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000031">
        <property type="entry name" value="LDLR chaperone MESD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019330">
        <property type="entry name" value="MESD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17600:SF2">
        <property type="entry name" value="LRP CHAPERONE MESD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17600">
        <property type="entry name" value="MESODERM DEVELOPMENT CANDIDATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10185">
        <property type="entry name" value="Mesd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026259973" evidence="7">
        <location>
            <begin position="19"/>
            <end position="179"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361392.3"/>
        </source>
    </evidence>
    <sequence length="179" mass="20741" checksum="35756D678FE341ED" modified="2020-08-12" version="1">MRASFILLCLALAPVVLAKKFTEEEKPAWAKKDIRDYSEADLERLLDQWDEDEEPLEPDELPEHLRPQPKIDLSNLDSKNPEDLLKVSKKGRTLMTFVSVTGNPTREEGETITKLWQTSLWNNHIQAERYMVDDNRAIFLFKDGGQAWEAKDFLVEQDRCKGVSIENKEYPGIHVKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV84</accession>
    <name>A0A6I8UV84_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA (guanine-9-)-methyltransferase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rswl</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361324.3">
        <property type="nucleotide sequence ID" value="XM_001361287.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV84"/>
    <dbReference type="FunCoup" id="A0A6I8UV84">
        <property type="interactions" value="1432"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804822"/>
    <dbReference type="InParanoid" id="A0A6I8UV84"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097745">
        <property type="term" value="P:mitochondrial tRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18102">
        <property type="entry name" value="Trm10_MRRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.30:FF:000003">
        <property type="entry name" value="tRNA methyltransferase 10C, mitochondrial RNase P subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025812">
        <property type="entry name" value="Trm10_C_MTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF5">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="12">
        <location>
            <begin position="181"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001361324.3"/>
        </source>
    </evidence>
    <sequence length="447" mass="52237" checksum="33434E0E5286D129" modified="2020-08-12" version="1">MLKTLRPWRLASHMLKGCAIPARRVSDSFKTFDGDNAFAGSEVNSKKFSNPFAQASPVISDGPAETEEDRGKRRKVLQLEADIAHQEGRRVPALDFFQDHHWDHVLSLPTKSARIKYYAFLWQINMKKEAERRKKAQRAEDTERRIADMRKEREENTHIIYGLAHTSMFLRIYDTTINHWQNNRLTRAMQFAPKMVLDCSYDKHMNNREASYAAKQLMLCFAENRLNDEPFDLHYCNAQLEGKCMQNLKRYIPTMLNPEFPMNVHKQCFTELFDKQNLVYLTPHCREDLVTYNPDDIYIVGAMVDTMNNEPLSLAKAKRLGLRMARLPLDRYLQWGSGSGKSLTLNQMINIMLDLKKTGNWDSALKHVPRRKVVDMDTQRRADDSRKALGTRARKLNVRIDHLLDFEEERRHTTAFATPQTLRQRKDGMEFQLDTWAAGKKKKKREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVA4</accession>
    <name>A0A6I8UVA4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Trypsin 3A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4804645</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361174.2">
        <property type="nucleotide sequence ID" value="XM_001361137.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVA4"/>
    <dbReference type="FunCoup" id="A0A6I8UVA4">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="GeneID" id="4804645"/>
    <dbReference type="KEGG" id="dpo:4804645"/>
    <dbReference type="InParanoid" id="A0A6I8UVA4"/>
    <dbReference type="OMA" id="FCAGTPQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077249">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000034">
        <property type="entry name" value="Eupolytin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026284014" evidence="6">
        <location>
            <begin position="19"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="30"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361174.2"/>
        </source>
    </evidence>
    <sequence length="265" mass="29229" checksum="B9CE4F74290AB252" modified="2020-08-12" version="1">MISLRLGISLWFIWTAGALNITAGQQDGRIVGGWETNIIHFPHQISLQLGTRHACGGAILSPTHILTAAHCVLEYSKPQYYVIRAGSSEWATGGSYIRIQRIVPHPKFHQPTHMNNDIALIQLQQPLVYSGNIQPIVLAARQDRIEPSAQLFVSGWGSQSISQMQPEKRLRYTVVQQRDQSQCIRNYFGAGTVTSSMFCAGTPQGGRDSCQGDSGGPLITSINGRMRLYGIVSWGLGCANAMFPGVYTRVSEYTDWIAQTMTEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXK0</accession>
    <name>A0A6I8UXK0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger FYVE domain-containing protein 26 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Sptz</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132794.2">
        <property type="nucleotide sequence ID" value="XM_002132758.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UXK0">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903076"/>
    <dbReference type="InParanoid" id="A0A6I8UXK0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXK0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000295">
        <property type="entry name" value="Zinc finger, FYVE domain-containing 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057946">
        <property type="entry name" value="TPR_ZFYVE26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028730">
        <property type="entry name" value="ZFYVE26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46591">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46591:SF1">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25569">
        <property type="entry name" value="TPR_ZFYVE26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="1436"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="391"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="522"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="398"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="522"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="542"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002132794.2"/>
        </source>
    </evidence>
    <sequence length="2256" mass="255891" checksum="340595F4CF22A15C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY12</accession>
    <name>A0A6I8UY12_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">eIF-4F 25 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6902494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133039.2">
        <property type="nucleotide sequence ID" value="XM_002133003.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY12"/>
    <dbReference type="FunCoup" id="A0A6I8UY12">
        <property type="interactions" value="1258"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902494"/>
    <dbReference type="InParanoid" id="A0A6I8UY12"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UY12">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002133039.2"/>
        </source>
    </evidence>
    <sequence length="219" mass="25221" checksum="9CC25F2325F0BB39" modified="2020-08-12" version="1">MVYTGYDGSALYPAMGGHMTISGDASPNEVRNLLDYGVLIKHPLEHTWTVWHWENDRSKNWSDMLNEVTSFNTVEDFFTVYYFIKPPSQLSVFNDYMVFKQGVRPMWEDDINKDGGRWIIMLDKNTKAMNDKLWQDLLLCSIGECFQHSNEINGVVINVRNKANKLSLWTRNSANAHAVLTLGQQLKGLLKLTNVEIQYQAHKDAMVQHGPNFNAMYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYT8</accession>
    <name>A0A6I8UYT8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Titin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133631.2">
        <property type="nucleotide sequence ID" value="XM_002133595.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYT8"/>
    <dbReference type="KEGG" id="dpo:6902100"/>
    <dbReference type="InParanoid" id="A0A6I8UYT8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UYT8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133631.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="693" mass="77741" checksum="E31C4C5FCC60B011" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0D5</accession>
    <name>A0A6I8V0D5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Polycystic kidney disease 2-like 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">brv3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polycystin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134773.2">
        <property type="nucleotide sequence ID" value="XM_002134737.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0D5"/>
    <dbReference type="FunCoup" id="A0A6I8V0D5">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900380"/>
    <dbReference type="InParanoid" id="A0A6I8V0D5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0D5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003915">
        <property type="entry name" value="PKD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051223">
        <property type="entry name" value="Polycystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046791">
        <property type="entry name" value="Polycystin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF183">
        <property type="entry name" value="AT14535P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20519">
        <property type="entry name" value="Polycystin_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01433">
        <property type="entry name" value="POLYCYSTIN2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="535"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="567"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin" evidence="9">
        <location>
            <begin position="169"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="8">
        <location>
            <begin position="379"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002134773.2"/>
        </source>
    </evidence>
    <sequence length="724" mass="86533" checksum="D55C7C6393AE74C4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0U6</accession>
    <name>A0A6I8V0U6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135106.3">
        <property type="nucleotide sequence ID" value="XM_002135070.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0U6"/>
    <dbReference type="KEGG" id="dpo:6900747"/>
    <dbReference type="InParanoid" id="A0A6I8V0U6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026312471" evidence="1">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135106.3"/>
        </source>
    </evidence>
    <sequence length="107" mass="12020" checksum="145A4D9EFA11499A" modified="2020-08-12" version="1">MRFPWLVLASCLSVADPGRAGAQRDEHYDVVGVSCGQDEVVSCMLVRCTEGGFCPAEQYCYDPKCVCRRGYRRVRGACLPKHTDHHLDSDALNSALLQAHFQENFHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1Y2</accession>
    <name>A0A6I8V1Y2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc carboxypeptidase A 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">LOC6896745</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <strain evidence="18">MV2-25</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <strain evidence="18">MV2-25</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the digestion of the blood meal.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002136952.1">
        <property type="nucleotide sequence ID" value="XM_002136916.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038847.1">
        <property type="nucleotide sequence ID" value="XM_015183361.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1Y2"/>
    <dbReference type="GeneID" id="6896745"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Zn peptidases"/>
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        <property type="entry name" value="FI16720P1"/>
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        <property type="entry name" value="CRBOXYPTASEA"/>
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        <property type="entry name" value="Protease propeptides/inhibitors"/>
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        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
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    </dbReference>
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        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19 20" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634470" description="Zinc carboxypeptidase A 1" evidence="16">
        <location>
            <begin position="20"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="17">
        <location>
            <begin position="132"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="15">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="ARBA" id="ARBA00023049"/>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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            <dbReference type="ARBA" id="ARBA00057299"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_002136952.1"/>
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    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015038847.1"/>
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    <sequence length="434" mass="49233" checksum="BBA923DC187D4346" modified="2020-08-12" version="1">MLWITRLLVLLLWLVLTFGQLGVPAQPQSQAQARYDHTRIYQVELASADHVQLFQALELASDSCSFMGHARVPGQKLTIMVTSHKVADFDDLLQSYNVSHRILNHNFQALIDANYREVAPEGTPPDQFDWKRYFQLDSIYAWLEFLAQQRPEVVTLVDLGASTQGLPIRGVRIAFGGENLTSVFVESGIHAREWIAPATATYFIEQLLNSKDAAVQTLARSQNWVIFPTVNPDGYRYTFKGDRMWRKNRSLFGICRGVDLNRNFPFHWNATGASGDPCRYDYSGPSAGSEVETQRIVQFLENRIQSERIRTFISLHSYSQLIMFPYGHTPERVENYQDLKEIGQLAAQQIKKQSGRIYRSGSIYETIYPSSGGSKDWVHGHLKVPITFTYELRGPPDSEDLFILSAGEIESTASEAFAALQTIVEEAGKRGYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V229</accession>
    <name>A0A6I8V229_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Cell cycle checkpoint protein RAD17 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Rad17</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad17/RAD24 family.</text>
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        <property type="nucleotide sequence ID" value="XM_002136913.4"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="term" value="P:DNA damage checkpoint signaling"/>
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    </dbReference>
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        <property type="term" value="P:DNA repair"/>
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        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002136949.4"/>
        </source>
    </evidence>
    <sequence length="531" mass="58831" checksum="B275EC59B86E2451" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2N7</accession>
    <name>A0A6I8V2N7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Asparagine synthetase domain-containing protein CG17486</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6899750</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135786.2">
        <property type="nucleotide sequence ID" value="XM_002135750.3"/>
    </dbReference>
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        <property type="interactions" value="1415"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899750"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V2N7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="5">
        <location>
            <begin position="57"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Asparagine synthetase" evidence="4">
        <location>
            <begin position="293"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002135786.2"/>
        </source>
    </evidence>
    <sequence length="573" mass="65313" checksum="B8DC5A9E13803839" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2V4</accession>
    <name>A0A6I8V2V4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisome assembly protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6903707</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002136767.2">
        <property type="nucleotide sequence ID" value="XM_002136731.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2V4"/>
    <dbReference type="FunCoup" id="A0A6I8V2V4">
        <property type="interactions" value="1537"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903707"/>
    <dbReference type="InParanoid" id="A0A6I8V2V4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16451">
        <property type="entry name" value="mRING_PEX12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000634">
        <property type="entry name" value="Peroxisome assembly protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038074">
        <property type="entry name" value="Peroxisome_assembly_p12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="15">
        <location>
            <begin position="24"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002136767.2"/>
        </source>
    </evidence>
    <sequence length="295" mass="34221" checksum="1EFA62111E4ED8D9" modified="2020-08-12" version="1">MAEAANVRQNLQNVPSIFEISAAETLDNLIYPALSKIFDYIGLRLDFKRWGNLRVREELSPLLTWIHLYLRNRASSFGESFYGLQRTATGTGELLTRPQQFTSATLLTFLPYIERKLRSRSAQHEDTYAWESYLINLFHAYNASKAVHTFAYLIKQASSHSPLFRAMRLTLRYPSEPPQEVKWTYIFLKMLEVLAFFLQFIQWWYSKDQRRKVGGTLQNPEAMQKRDLPEEVKQTLPQTGKCPICLLPLQTPTACSVSGYVFCWKCIISHMKEHGTCPVTSYPISLDDLVRIYET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V366</accession>
    <name>A0A6I8V366_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137639.3">
        <property type="nucleotide sequence ID" value="XM_002137603.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V366"/>
    <dbReference type="KEGG" id="dpo:6897490"/>
    <dbReference type="InParanoid" id="A0A6I8V366"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF67">
        <property type="entry name" value="COREPRESSOR OF PANGOLIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="1">
        <location>
            <begin position="17"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137639.3"/>
        </source>
    </evidence>
    <sequence length="252" mass="29540" checksum="120CCCB3BE84CE2A" modified="2020-08-12" version="1">MNSYVKIHMWSDENTLFLIKLVKNSRYVWDSTHVYYKQKTKRQNFWLEAAKKMNRETGLKMEVGEVKKKWQNIRAYYYAERNKLMKATRSGTQDGEVRSVWKYMDEMSFMRDCRIPPGYNPDTSVSQTSTIYNDGEEESYDEEKFPIEEADCSVQEQLEFCAPWSPDASKKRKRSDSESNDEILNKLSDSHKNLTDVLAMPTTPNPHADFGAFVGAELKKLDEDLGKRLEIKILQLITDYKIKQLGRDDEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V370</accession>
    <name>A0A6I8V370_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Poly(U)-specific endoribonuclease homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EndoU</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ENDOU family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137707.1">
        <property type="nucleotide sequence ID" value="XM_002137671.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V370"/>
    <dbReference type="SMR" id="A0A6I8V370"/>
    <dbReference type="GeneID" id="6897562"/>
    <dbReference type="KEGG" id="dpo:6897562"/>
    <dbReference type="InParanoid" id="A0A6I8V370"/>
    <dbReference type="OMA" id="ERNDGGW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248737">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21159">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039787">
        <property type="entry name" value="ENDOU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018998">
        <property type="entry name" value="EndoU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439:SF42">
        <property type="entry name" value="ENDORIBONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439">
        <property type="entry name" value="PLACENTAL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09412">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="EndoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51959">
        <property type="entry name" value="ENDOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
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    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="EndoU" evidence="13">
        <location>
            <begin position="55"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002137707.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="37133" checksum="D50B683EA19488C9" modified="2020-08-12" version="1">MDAVQQNTNTNNNSNIDSDKCQKLKRFVIVGLLITIGILSWHFYEYFQSKPLPKAPDDVLALSKSLYAEETVVSPYLYKVNLQGKTTSGARDDKAPKNLFELHQDLLARDANSTTALLLRLFDNYELDVAAAEHTTAEHEAEQHDFLRAVMNTRVMKLTMRFLVHKDIVTSDYDDQLRLLQELWFTPYSRGRGIVGSSSFEHVFMAELRDQKVLGLHNWIYFADQEQRGNIDYKGWLSHLEMGKPNQISLSLRNTFYGIQKPVTGFFVGTSPELEMSLYTACFLATPEKDPCHIQLGHARVSIVSHSWNWNGKRLIATAYPDNLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3C2</accession>
    <name>A0A6I8V3C2_DROPS</name>
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        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC6896992</name>
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    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="entry name" value="DUF1091"/>
    </dbReference>
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        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026304070" evidence="1">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137178.3"/>
        </source>
    </evidence>
    <sequence length="182" mass="21346" checksum="E169D974D30F85E5" modified="2020-08-12" version="1">MRCQLMICAVLGLFLTFPIKINDAVVFKFTNIVGESYNQSWFVYHNYRLKAVSRNKVLLNINGTILHPVNDASIHGRIFKKANGYKPWLFDIYIDACQYLRKRNQPVANIVYGLFKQFSNINHSCPYVGPQIIKDFYLRPELLRIPMPTGDYLLALQWYFDKKLQFDTNVSFTFVEDLMKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V402</accession>
    <name>A0A6I8V402_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138346.2"/>
        </source>
    </evidence>
    <sequence length="343" mass="39010" checksum="E03BF7277835AA0E" modified="2020-08-12" version="1">MYQIYAVFIGLGCLISVLPFIVHGIPLSLTLGEPLNASSNQSSVPPNMTVPSKSEAVQAQVQLQDLEAAADNSGYRGQEPESGKMGLFPVAYQSKLRTADKAPATTKVSATKRPMVRGNLTKGALNFWAMPKAKKPKAKGEGEAPAKASPPAPAPAISPRAGEGKIRTKSFEKTISIPRMESDHMGNWVFNPWGVTFGVLWQDTRMHYKIKNSMSEIIMDTPYHGHYQMYYNKPQTQVCRGLSTIYNKPKVYKWLQRRILRNVTPNFHVFSSYRPIWLDFRRELFKMGRSEACHVSAMDDWLLYNNCAILRNMRMEYLIPKYPLHQYGYYHPQMRVELPYKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V497</accession>
    <name>A0A6I8V497_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6898452</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138495.2">
        <property type="nucleotide sequence ID" value="XM_002138459.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V497">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898452"/>
    <dbReference type="InParanoid" id="A0A6I8V497"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016540">
        <property type="term" value="P:protein autoprocessing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10144">
        <property type="entry name" value="Peptidase_S74_CIMCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051577">
        <property type="entry name" value="MRF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026932">
        <property type="entry name" value="MYRF_ICA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030392">
        <property type="entry name" value="S74_ICA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13029">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13029:SF18">
        <property type="entry name" value="MYELIN REGULATORY FACTOR HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13887">
        <property type="entry name" value="MYRF_ICA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13884">
        <property type="entry name" value="Peptidase_S74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51688">
        <property type="entry name" value="ICA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="NDT80" evidence="8">
        <location>
            <begin position="321"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S74" evidence="9">
        <location>
            <begin position="633"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="6">
        <location>
            <begin position="321"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="42"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="911"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="960"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1101"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="927"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="980"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1101"/>
            <end position="1110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002138495.2"/>
        </source>
    </evidence>
    <sequence length="1466" mass="159928" checksum="A6814F2D8E7E5746" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V504</accession>
    <name>A0A6I8V504_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CLIP domain-containing serine protease</fullName>
            <ecNumber evidence="11">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC6899117</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The clip domain consists of 35-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_002139034.3">
        <property type="nucleotide sequence ID" value="XM_002138998.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V504"/>
    <dbReference type="KEGG" id="dpo:6899117"/>
    <dbReference type="InParanoid" id="A0A6I8V504"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000078">
        <property type="entry name" value="Serine protease H137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022700">
        <property type="entry name" value="CLIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038565">
        <property type="entry name" value="CLIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12032">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00680">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026374975" description="CLIP domain-containing serine protease" evidence="12">
        <location>
            <begin position="24"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="112"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002139034.3"/>
        </source>
    </evidence>
    <sequence length="366" mass="40526" checksum="A18771B1669B61F5" modified="2020-08-12" version="1">MIGCVSGSLTFCLLAALIGSGLGAEDYPDYCSTFEGWPGWCVHYLECPSINALVQKNLSGHELSKMETDMWNQARCSDRNTTYVCCQDEINASGLEMLTNETECGKFTLFKVTNGQRVKMGSRPWMALLKYNVATMARHQFLCGATLISKRFVLTAAHCSINELPIAVRLGEHDITTDEDCIMVGSKKRCQLPHQDIGIEKIIMHEGYPKNITQNDIALIKLDRDVVYSAHINSICLPLYPSLQKNVLGQTLYLVTGWGRTESGRPSNVPMEAFVKHLSMEDCIKAYRGYAFAENQVCTSAGIQDSCSGDSGGPLQYPFRFYGKQRFVQAGIVSVGSRQCGIETYPSVATNVVVFMPWITKQMAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V681</accession>
    <name>A0A6I8V681_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6898985</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138945.3">
        <property type="nucleotide sequence ID" value="XM_002138909.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V681">
        <property type="interactions" value="450"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898985"/>
    <dbReference type="InParanoid" id="A0A6I8V681"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002683">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="773"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="827"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="885"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="914"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="943"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="972"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="1000"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="369"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="472"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="536"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="689"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="736"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1017"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1227"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1388"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1496"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1547"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="472"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="536"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="691"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="705"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="739"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="754"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1063"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1117"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1157"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1187"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1227"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1259"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1293"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1307"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1336"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1352"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1396"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1443"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1496"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1557"/>
            <end position="1566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002138945.3"/>
        </source>
    </evidence>
    <sequence length="1633" mass="181675" checksum="27DB64E7C378768E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6A6</accession>
    <name>A0A6I8V6A6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="6">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">eIF6</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1. Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <text evidence="6">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736952.1">
        <property type="nucleotide sequence ID" value="XM_003736904.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6A6"/>
    <dbReference type="FunCoup" id="A0A6I8V6A6">
        <property type="interactions" value="1501"/>
    </dbReference>
    <dbReference type="GeneID" id="4803398"/>
    <dbReference type="KEGG" id="dpo:4803398"/>
    <dbReference type="CTD" id="3692"/>
    <dbReference type="InParanoid" id="A0A6I8V6A6"/>
    <dbReference type="OMA" id="WCAFCGM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074606">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
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    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_003736952.1"/>
        </source>
    </evidence>
    <sequence length="245" mass="26459" checksum="5A8D5CA497E85341" modified="2020-08-12" version="1">MALRVQFENNDDIGVFTKLTNTYCLVAIGGSETFYSAFEAELAETIPVVHANVGGCRIIGRLTVGNRNGLLVPNSTTDEELQHLRNSLPDAVKIQRVEERLSALGNVVACNDYVALVHPDLDKETEEIIGDVLNVEVFRQTIADNTLVGSYTVLSNQGGMVHPKTSIQDQDELSSLLQVPLVAGTVNRGSEVLAAGMVVNDWLSFVGMNTTATEISVIESVFKLNQAQPAAVTTKLRAALIEDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7H5</accession>
    <name>A0A6I8V7H5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein Turandot X-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC26532717</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Turandot family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035447.2">
        <property type="nucleotide sequence ID" value="XM_015179961.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V7H5"/>
    <dbReference type="KEGG" id="dpo:26532717"/>
    <dbReference type="InParanoid" id="A0A6I8V7H5"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V7H5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010825">
        <property type="entry name" value="Turandot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07240">
        <property type="entry name" value="Turandot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026124281" evidence="8">
        <location>
            <begin position="22"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015035447.2"/>
        </source>
    </evidence>
    <sequence length="158" mass="17826" checksum="AE8057F59FDBBFC5" modified="2020-08-12" version="1">MGHLIRWSCLLIVLVCSGTRGDDDQYGTAGRHLLAIYSNPDVNRATKERNLREMAQFYDAYQDQIGLTSEERLRAEELRKRYTEAIQTGVLAEGETSREGTIGPIVAKYIVEGVVDLAKVVESHRSSSAKGIEINRIITIILFIFCLFSSGMFRSKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAW3</accession>
    <name>A0A6I8VAW3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26534406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037393.2">
        <property type="nucleotide sequence ID" value="XM_015181907.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAW3"/>
    <dbReference type="KEGG" id="dpo:26534406"/>
    <dbReference type="InParanoid" id="A0A6I8VAW3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031934">
        <property type="entry name" value="DUF4769"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15992">
        <property type="entry name" value="DUF4769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015037393.2"/>
        </source>
    </evidence>
    <sequence length="297" mass="31819" checksum="50F8C31C223070C7" modified="2020-08-12" version="1">MVKNDAANTTNLSGTMDELEIKITYKNKVATPEEPLGFFLGIQMKQKTPSKASSSASAAQQAGNATVIRQIQTSAAQQAGNATVIRQIQTSAAQQAGNATAIRQIQTSAAQQAGNATAIRQIQASAAQQAGNATAIRQIQARTPSFSHSAGSGVRKPSPASTAAVILRAVGATALLQRVGRPEPLTPNLRRTAINAIADHFVIKKIPLSISNCRNLESELLSLFPGEQESDYTVSGRSRMYVRYNNRKRLEKHHANIRRKLIWTPATTEPLTPKTSVASGSQTLPVAFDEVPDAREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBC8</accession>
    <name>A0A6I8VBC8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA-binding protein FUS isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037646.2">
        <property type="nucleotide sequence ID" value="XM_015182160.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBC8"/>
    <dbReference type="FunCoup" id="A0A6I8VBC8">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VBC8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079100">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VBC8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015037646.2"/>
        </source>
    </evidence>
    <sequence length="479" mass="48999" checksum="A17C6DB7C1AB4175" modified="2020-08-12" version="1">MEGAQVSSPSVGGPRGGGFRGRGGGAPMRGGFDRGRGRGGRGHFMGGMRGGMGGGPPGMQGMMPGPPRGMRGPRGGMGYQGRGGFQPRGGAPMGMRGGRPPMYQQPPKQHTGADNNKTVSPVPTTAAPGEAQVGEAAGEQETTDSQAAAPASVSNNSHSSSAPGSNGGGGGGGGPPPYLGRGRGRGGLFSGRGRGGGYNPHMNGSGGGGGMYGGPHSHHSNSMGGGPGGDHGQMPRRGAPRGRGGYNQGMSRPPMHHHQAHNPNTQIAPVPALKRGAPGGPGPKRGRYEGGPYGQRPMTPKYHNSQQHMGGGGGGMASQSSYGSAPSHHSSAQSHHGGYQTHDQTQQVDPYAQSSYSTSTSQQGYNGYGQSSSSYAAHTSQTSAPASSSYATHAAEYDQSQYQSYNSTGYAAQQDTRYPSYSQDYSQQYGSTAVDYNATGQTDYSQHGQQSAAGYDERAYAGYDSQAYSQNYSNAAAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDN9</accession>
    <name>A0A6I8VDN9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Presequence protease, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4803331</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M16 family. PreP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015038986.2">
        <property type="nucleotide sequence ID" value="XM_015183500.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDN9"/>
    <dbReference type="FunCoup" id="A0A6I8VDN9">
        <property type="interactions" value="1825"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803331"/>
    <dbReference type="InParanoid" id="A0A6I8VDN9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000013">
        <property type="entry name" value="Mitochondrial presequence protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000009">
        <property type="entry name" value="Presequence protease, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000011">
        <property type="entry name" value="Presequence protease, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013578">
        <property type="entry name" value="Peptidase_M16C_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055130">
        <property type="entry name" value="PreP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016">
        <property type="entry name" value="PRESEQUENCE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43016:SF13">
        <property type="entry name" value="PRESEQUENCE PROTEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08367">
        <property type="entry name" value="M16C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22516">
        <property type="entry name" value="PreP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01264">
        <property type="entry name" value="M16C_associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16C associated" evidence="12">
        <location>
            <begin position="530"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015038986.2"/>
        </source>
    </evidence>
    <sequence length="1034" mass="118264" checksum="B69DB383634FE12E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGC3</accession>
    <name>A0A6I8VGC3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial proton/calcium exchanger protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Leucine zipper-EF-hand-containing transmembrane protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25 26 27 28 29 30" type="primary">Letm1</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 27 29">MV-25-SWS-2005</strain>
            <strain evidence="24">MV2-25</strain>
            <tissue evidence="25 27 29">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="26 28 30" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26 28 30">MV-25-SWS-2005</strain>
            <strain evidence="24">MV2-25</strain>
            <tissue evidence="26 28 30">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LETM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361972.2">
        <property type="nucleotide sequence ID" value="XM_001361935.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040732.1">
        <property type="nucleotide sequence ID" value="XM_015185246.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234360.1">
        <property type="nucleotide sequence ID" value="XM_033378469.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234361.1">
        <property type="nucleotide sequence ID" value="XM_033378470.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234363.1">
        <property type="nucleotide sequence ID" value="XM_033378472.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234364.1">
        <property type="nucleotide sequence ID" value="XM_033378473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGC3"/>
    <dbReference type="GeneID" id="4805592"/>
    <dbReference type="KEGG" id="dpo:4805592"/>
    <dbReference type="OMA" id="HGFRHLH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078285">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
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    <sequence length="1034" mass="115556" checksum="E2AE2FFBE4909284" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIL9</accession>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <reaction evidence="1 13">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
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        <text evidence="2 13">Belongs to the eukaryotic diacylglycerol kinase family.</text>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015041870.2"/>
        </source>
    </evidence>
    <sequence length="1501" mass="164311" checksum="E0E1AA05887C76B5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKF4</accession>
    <name>A0A6I8VKF4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26534321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042803.2">
        <property type="nucleotide sequence ID" value="XM_015187317.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VKF4"/>
    <dbReference type="KEGG" id="dpo:26534321"/>
    <dbReference type="InParanoid" id="A0A6I8VKF4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015042803.2"/>
        </source>
    </evidence>
    <sequence length="184" mass="20510" checksum="64B6FB899C5305DB" modified="2020-08-12" version="1">MGYRTPRLVWAAHFSPTRDDDSGSRISYQSVGLECGETIPQPQTGTLPADRAQCDRSEKANGHTPIGASIRGFGLKFSVSFAALEVFPFKISWRILFFRAPSGVWVLPDGYCIYDRVHRASRCYLHTLRRTTSIRRDPLWPQADMPRSPPLSSRLPPSDARNTSGVTVDTVMTTIAYLEGDASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VL15</accession>
    <name>A0A6I8VL15_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Unc-112-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">Fit2</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the kindlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352436.2">
        <property type="nucleotide sequence ID" value="XM_001352400.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043563.2">
        <property type="nucleotide sequence ID" value="XM_015188077.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VL15"/>
    <dbReference type="KEGG" id="dpo:4812007"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VL15">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13205">
        <property type="entry name" value="FERM_C_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17095">
        <property type="entry name" value="FERM_F0_kindlins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17096">
        <property type="entry name" value="FERM_F1_kindlins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01237">
        <property type="entry name" value="PH_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000037">
        <property type="entry name" value="Fermitin family homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000502">
        <property type="entry name" value="Unc-112-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040790">
        <property type="entry name" value="Kindlin_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037837">
        <property type="entry name" value="PH_Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF13">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18124">
        <property type="entry name" value="Kindlin_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="403"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015043563.2"/>
        </source>
    </evidence>
    <sequence length="711" mass="81154" checksum="EBB7B2B2CE6C491D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLZ0</accession>
    <name>A0A6I8VLZ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cuticle protein 16.5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015044031.2">
        <property type="nucleotide sequence ID" value="XM_015188545.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLZ0"/>
    <dbReference type="KEGG" id="dpo:26533203"/>
    <dbReference type="InParanoid" id="A0A6I8VLZ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054721">
        <property type="entry name" value="GEO12453p1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685:SF2">
        <property type="entry name" value="825-OAK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685">
        <property type="entry name" value="825-OAK-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22861">
        <property type="entry name" value="GEO12453p1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026124284" description="Cuticle protein 16.5" evidence="1">
        <location>
            <begin position="18"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015044031.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="10171" checksum="A955675A9003300E" modified="2020-08-12" version="1">MFKFAAVIFAVLACASAKPGFLSAPLAYTAPAAIVAAPAPVVTATSSQVFARNYNGIAAAPVIAPVAAPLAAPVLAKYAAAPLANPLAYRSVPLAYSAPLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNT4</accession>
    <name>A0A6I8VNT4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Probable cytochrome P450 12a4, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6896994</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232725.1">
        <property type="nucleotide sequence ID" value="XM_033376834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNT4"/>
    <dbReference type="KEGG" id="dpo:6896994"/>
    <dbReference type="InParanoid" id="A0A6I8VNT4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11054">
        <property type="entry name" value="CYP24A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF120">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033232725.1"/>
        </source>
    </evidence>
    <sequence length="526" mass="60032" checksum="F89665DC18DA0FB8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VP72</accession>
    <name>A0A6I8VP72_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein argonaute-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6904109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232827.1">
        <property type="nucleotide sequence ID" value="XM_033376936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VP72"/>
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        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6904109"/>
    <dbReference type="InParanoid" id="A0A6I8VP72"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="381"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="655"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232827.1"/>
        </source>
    </evidence>
    <sequence length="983" mass="112705" checksum="B2B229CE0722EC89" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPE1</accession>
    <name>A0A6I8VPE1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein sarah</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sra</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RCAN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232644.1">
        <property type="nucleotide sequence ID" value="XM_033376753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPE1"/>
    <dbReference type="SMR" id="A0A6I8VPE1"/>
    <dbReference type="FunCoup" id="A0A6I8VPE1">
        <property type="interactions" value="1024"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802873"/>
    <dbReference type="InParanoid" id="A0A6I8VPE1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008597">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007617">
        <property type="term" value="P:mating behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12434">
        <property type="entry name" value="RRM_RCAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000092">
        <property type="entry name" value="Calcipressin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006931">
        <property type="entry name" value="Calcipressin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300">
        <property type="entry name" value="CALCIPRESSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300:SF14">
        <property type="entry name" value="PROTEIN SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04847">
        <property type="entry name" value="Calcipressin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033232644.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="31935" checksum="D59EA2FF2356A5D7" modified="2020-08-12" version="1">MSDAAKSNNNAAAASSPEPAPATTTTDEVLPTKDAASSPTTPRGGNNHNNNHSKSHNKLKSTQNSSGGGSIDKLSPDQDIYINAADGLPSQHPSLPKEGIIDSDTEPEVDADSFDDLPTSIIVTNIHSEVFANPELKHGMEELFRTFSESATFQWLRSFRRLRVNYDNAIAAANARIKLHQYEFNKKTVITCYFAQPVTPVNNKNLQPPAPVKQFLISPPASPPAGWEPREEAEPLVNHDLLAALASLTPGESHELHPQSEDQPAIIVHTAMVPEGAGGPGIQPKAPIVQTKCPERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VR77</accession>
    <name>A0A6I8VR77_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233576.1">
        <property type="nucleotide sequence ID" value="XM_033377685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VR77"/>
    <dbReference type="KEGG" id="dpo:117183543"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070388">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VR77">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026213761" evidence="1">
        <location>
            <begin position="29"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233576.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="24443" checksum="9C3E15069E18515E" modified="2020-08-12" version="1">MHFTKTRSCRSTMPLMLLLIVLTAGAWCASSSKPLEKRSLALDEASIPWAITSGPRALNGGAWNLGWPTWSGGHTSLGIGPTPNEVAHAISVAKQASANVLIAQQHLQAAKENVLNQQRIATERQTQAVILKQKSQAAAAIQRSEAIQQRLAASKAAVAHAAAHAAAAEIQKTEHEAAKLGHFTRNAHPGVGNPLSTIGSGNNNFSPSNVEAIGFGHWLSGSTAGKSVNNWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VS37</accession>
    <name>A0A6I8VS37_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ran-binding proteins 9/10 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RanBPM</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RANBP9/10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233867.1">
        <property type="nucleotide sequence ID" value="XM_033377976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VS37"/>
    <dbReference type="FunCoup" id="A0A6I8VS37">
        <property type="interactions" value="1551"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805342"/>
    <dbReference type="InParanoid" id="A0A6I8VS37"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VS37">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd12909">
        <property type="entry name" value="SPRY_RanBP9_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000011">
        <property type="entry name" value="RAN binding protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035782">
        <property type="entry name" value="SPRY_RanBP9/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="29"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="285"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="440"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033233867.1"/>
        </source>
    </evidence>
    <sequence length="626" mass="69534" checksum="CA14CF8FADCC82E8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VS39</accession>
    <name>A0A6I8VS39_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4805623</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233718.1">
        <property type="nucleotide sequence ID" value="XM_033377827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VS39"/>
    <dbReference type="FunCoup" id="A0A6I8VS39">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805623"/>
    <dbReference type="InParanoid" id="A0A6I8VS39"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238:SF58">
        <property type="entry name" value="FI22604P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="67"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033233718.1"/>
        </source>
    </evidence>
    <sequence length="580" mass="65753" checksum="FA6211CB49AAEEE8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VUQ4</accession>
    <name>A0A6I8VUQ4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helicase domino-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183693</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234369.1">
        <property type="nucleotide sequence ID" value="XM_033378478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VUQ4"/>
    <dbReference type="KEGG" id="dpo:117183693"/>
    <dbReference type="InParanoid" id="A0A6I8VUQ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033234369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="31115" checksum="15D1EAB34A049F89" modified="2020-08-12" version="1">MSFNINFNCGPPPVGQNVYKIGELEGEKNIITKESILECINSLPKCENLTLQQKPEVGCQQTANADDSFDCSNLTAAQLEQLALLTAAANPGQSSVSLMVMIPVESLLDIVQVLSGIAAQIVSMSSTVLKGNSSKGTTSEVPIWVDAGVGVPPSVGKIDMEMYPSPQQEEHYYPQHMAAQEDKYQQSPADKPSVVVQTQSKSTHNPSFRILEHVIIPSQQPGSIGSLCKQEQQQKATTAGGSAQCYWRVVAVGLRFSSFLFCRCCVDAVGLRFSSFRSFHCRCFVAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXG4</accession>
    <name>A0A6I8VXG4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC4816859</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033235785.1">
        <property type="nucleotide sequence ID" value="XM_033379894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXG4"/>
    <dbReference type="FunCoup" id="A0A6I8VXG4">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816859"/>
    <dbReference type="InParanoid" id="A0A6I8VXG4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VXG4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000072">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000130">
        <property type="entry name" value="Protein kinase, cGMP-dependent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000827">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF144">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0140">cGMP</keyword>
    <keyword evidence="9" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="7 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="14">
        <location>
            <begin position="434"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="14">
        <location>
            <begin position="552"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="695"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="953"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="65"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="262"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="342"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="663"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="345"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="663"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033235785.1"/>
        </source>
    </evidence>
    <sequence length="1004" mass="112766" checksum="EAEB47C7CF29CA71" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXJ4</accession>
    <name>A0A6I8VXJ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Proc isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Proc</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235289.1">
        <property type="nucleotide sequence ID" value="XM_033379398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXJ4"/>
    <dbReference type="FunCoup" id="A0A6I8VXJ4">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VXJ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080102">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VXJ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235289.1"/>
        </source>
    </evidence>
    <sequence length="190" mass="21341" checksum="A4E6E57808B4863D" modified="2020-08-12" version="1">MNETSFKRATPRRPQPQERPPLHQQKTPTMRAARSSQTGTERESERCLIMGMGLNLRQGHRWLVWLLLLLLAIPPQMVDGRYLPTRSHGDDLDKLRELMLQILELSNEDPQQQQQQQQQQTMPQQHPLLRLHNEANSGSSSTAGGSSNGNNPRVSNGNSNAAWLQKLSAMGALDELGGDAPRMGPNYGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXQ5</accession>
    <name>A0A6I8VXQ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Schwannomin-interacting protein 1 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">Schip1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235758.1">
        <property type="nucleotide sequence ID" value="XM_033379867.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235759.1">
        <property type="nucleotide sequence ID" value="XM_033379868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXQ5"/>
    <dbReference type="KEGG" id="dpo:26532178"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035332">
        <property type="term" value="P:positive regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039045">
        <property type="entry name" value="SCHIP_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015649">
        <property type="entry name" value="SCHIP_1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13103:SF2">
        <property type="entry name" value="IQCJ-SCHIP1 READTHROUGH TRANSCRIPT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13103">
        <property type="entry name" value="SCHWANNOMIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10148">
        <property type="entry name" value="SCHIP-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Schwannomin interacting protein 1 C-terminal" evidence="2">
        <location>
            <begin position="338"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235759.1"/>
        </source>
    </evidence>
    <sequence length="550" mass="63561" checksum="D84C2E3E50060BA0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYG2</accession>
    <name>A0A6I8VYG2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236028.1">
        <property type="nucleotide sequence ID" value="XM_033380137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYG2"/>
    <dbReference type="KEGG" id="dpo:6902492"/>
    <dbReference type="InParanoid" id="A0A6I8VYG2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236028.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="24141" checksum="3C30B191A8AF7FFF" modified="2020-08-12" version="1">MDTQLKAEILPDSPEQKALVKEMKRVELQLQNLDRKTLKNENENVVQHQPASPRWIRITPTFKCFTQPWVSAKRRGAVVTACRGSIHDPIRAISRRAFRTPLFFILDHLFFIPKYVSQPFSGSLRPSATYSFCMSTFSASPLTIAVTEKQKKALVKEMKRVELQLQNLDRKTLKNENENVAQHQPASPRWSRITPTVTTSLTLGSPPSAAALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W111</accession>
    <name>A0A6I8W111_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">alien</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033237036.1">
        <property type="nucleotide sequence ID" value="XM_033381145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W111"/>
    <dbReference type="FunCoup" id="A0A6I8W111">
        <property type="interactions" value="2552"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W111"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W111">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000001">
        <property type="entry name" value="Putative cop9 signalosome complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058796">
        <property type="entry name" value="COPS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25983">
        <property type="entry name" value="COPS2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="258"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033237036.1"/>
        </source>
    </evidence>
    <sequence length="447" mass="51893" checksum="5B8214F12885AA3F" modified="2020-08-12" version="1">MSDNDDDFMCDDDEDYGLEYSEDSNSEPDVDLENQYYNSKALKEEEPKAALASFQKVLDLENGEKGEWGFKALKQMIKINFRLLPQCNYDEMMVRYKQLLTYIKSAVTRNHSEKSINSILDYISTSKNMALLQNFYETTLDALRDAKNDRLWFKTNTKLGKLYFDRSDFTKLQKILKQLHQSCQTDDGEDDLKKGTQLLEIYALEIQMYTVQKNNKKLKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGEFEKAHTDFFEAFKNYDESGSPRRTTCLKYLVLANMLMKSGINPFDSQEAKPYKNDPEILAMTNLVNSYQNNDINEFETILRQHRSNIMADQFIREHIEDLLRNIRTQVLIKLIRPYKNIFIPFIASALNIEPAEVESLLVSCILDDTIKGRIDQVNQVLQLDKVNSSAARYNALEKWSNQIHSLQFAVVQKMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W2H5</accession>
    <name>A0A6I8W2H5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117184416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237532.1">
        <property type="nucleotide sequence ID" value="XM_033381641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W2H5"/>
    <dbReference type="KEGG" id="dpo:117184416"/>
    <dbReference type="InParanoid" id="A0A6I8W2H5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033237532.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="12706" checksum="60E1823A7612B2DF" modified="2020-08-12" version="1">MLKLFLYEDRWVKVKHNPIINIIAPPEKRLVDVRMRSQDHILVWKCLSGMTNNFPMKQCHISFCCSIFHVDHVKCIINNGNVPDSFHPARHSPAAAIPLPDVRSYITRCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W582</accession>
    <name>A0A6I8W582_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mucin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC26533159</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238493.1">
        <property type="nucleotide sequence ID" value="XM_033382602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W582"/>
    <dbReference type="KEGG" id="dpo:26533159"/>
    <dbReference type="InParanoid" id="A0A6I8W582"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272146">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026214820" evidence="2">
        <location>
            <begin position="25"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="911"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="838"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="868"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033238493.1"/>
        </source>
    </evidence>
    <sequence length="972" mass="100393" checksum="AC4FF10F697F5DC3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6L9</accession>
    <name>A0A6I8W6L9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial thiamine pyrophosphate carrier</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 25 member 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Tpc1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mitochondrial transporter mediating uptake of thiamine diphosphate into mitochondria. It is not clear if the antiporter activity is affected by the membrane potential or by the proton electrochemical gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>thiamine phosphate(out) + thiamine diphosphate(in) = thiamine phosphate(in) + thiamine diphosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:73383"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239001.1">
        <property type="nucleotide sequence ID" value="XM_033383110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6L9"/>
    <dbReference type="FunCoup" id="A0A6I8W6L9">
        <property type="interactions" value="436"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897925"/>
    <dbReference type="InParanoid" id="A0A6I8W6L9"/>
    <dbReference type="OMA" id="MYVCYGA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247556">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090422">
        <property type="term" value="F:thiamine pyrophosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000011">
        <property type="entry name" value="Mitochondrial thiamine pyrophosphate carrier 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="23"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="131"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="230"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033239001.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="36329" checksum="F4382C4096921004" modified="2020-08-12" version="1">MAVTTGTTTTSPSPSPRRRHSTREQLHQIVAGGLSAAITRSTCQPLDVLKIRFQLQVEPLGKSQSTGTRQASKYISITQAVKTIYREEGVLAFWKGHNPAQVLSIMYGICQFWTYEQLSLQANQTHYLKDHQHLSNFLCGAAAGGAAVIISTPLDVIRTRLIAQDTSKGYRNATRAVSAIVHQEGPRGMYRGLSSALLQIAPLMGTNFMAYRLFSDWACAFFEVGDRSKLPTWTLLALGASSGMLSKTIVYPFDLIKKRLQIQGFESNRQTFGQTLQCHGVWDCLRLTVRQEGVRGLYKGVAPTLLKSSLTTALYFSIYDKLKQVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7A0</accession>
    <name>A0A6I8W7A0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neurocalcin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Nca</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239236.1">
        <property type="nucleotide sequence ID" value="XM_033383345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W7A0"/>
    <dbReference type="SMR" id="A0A6I8W7A0"/>
    <dbReference type="FunCoup" id="A0A6I8W7A0">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184694"/>
    <dbReference type="InParanoid" id="A0A6I8W7A0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W7A0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF178">
        <property type="entry name" value="NEUROCALCIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033239236.1"/>
        </source>
    </evidence>
    <sequence length="190" mass="21894" checksum="F1158FEB8CF13F64" modified="2020-08-12" version="1">MGKQNSKLKPEVLEDLKQNTEFTDAEIQEWYKGFLKDCPSGHLSVEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFLCALSVTSRGKLEQKLKWAFSMYDLDGNGYISRQEMLEIVTAIYKMVGSVMKMPEDESTPEKRTDKIFRQMDRNKDGKLSLEEFIEGAKSDPSIVRLLQCDPQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7W4</accession>
    <name>A0A6I8W7W4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 10">B-cell CLL/lymphoma 6 member B protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10 11" type="primary">LOC4812417</name>
    </gene>
    <organism evidence="7 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9 10">MV-25-SWS-2005</strain>
            <tissue evidence="8 9 10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352898.3">
        <property type="nucleotide sequence ID" value="XM_001352862.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043962.2">
        <property type="nucleotide sequence ID" value="XM_015188476.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239371.1">
        <property type="nucleotide sequence ID" value="XM_033383480.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239372.1">
        <property type="nucleotide sequence ID" value="XM_033383481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W7W4"/>
    <dbReference type="KEGG" id="dpo:4812417"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076712">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W7W4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002232">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000143">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF110">
        <property type="entry name" value="AGAP003189-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="71"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="510"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="532"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="625"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="553"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="625"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352898.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015043962.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033239371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033239372.1"/>
        </source>
    </evidence>
    <sequence length="655" mass="70961" checksum="E0586895FC4287F0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAR7</accession>
    <name>A0A6I8WAR7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase aspartic putative domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239644.1">
        <property type="nucleotide sequence ID" value="XM_033383753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WAR7"/>
    <dbReference type="KEGG" id="dpo:117184812"/>
    <dbReference type="InParanoid" id="A0A6I8WAR7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="34512" checksum="024FE9252F3D485B" modified="2020-08-12" version="1">MAPRIRASRTTESRRDRGINSYRCRVCRGIHPLRTCHHFLRLSPEKRLRAVLINKYCGNCLAHQHSGQDCRSGGLCRVCGKNHHTLLHIPSSRRPVRSASGASSPSTAPHVKRRPRRPARSASAASSPSTSAHADRRPRLPVSRPVPGPPAPSVDSLLQHRSLIILPTALVVLDTGSKNFETAALIDPCTPVSCIDDSLASAFKLPTTCVGDEKVCTAVIRAKMGDFQLETMLKVEPRVRIRTPIRQLSDSVRARFDDLRLADEQFHRPATISLILGADVYPKVIQPGFLAREDGLPVAQSTVFGWIVSGACTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDX1</accession>
    <name>A0A6I8WDX1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Triple functional domain protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trio</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033241427.1">
        <property type="nucleotide sequence ID" value="XM_033385536.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8WDX1">
        <property type="interactions" value="845"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WDX1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271079">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WDX1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13240">
        <property type="entry name" value="PH1_Kalirin_Trio_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13241">
        <property type="entry name" value="PH2_Kalirin_Trio_p63RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide exchange factor DBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000023">
        <property type="entry name" value="Kalirin RhoGEF kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000341">
        <property type="entry name" value="Trio, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000390">
        <property type="entry name" value="Trio, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000040">
        <property type="entry name" value="Trio, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047054">
        <property type="entry name" value="Kalirin_TRIO_PH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058918">
        <property type="entry name" value="KALRN/TRIO-like_spectrin"/>
    </dbReference>
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            <begin position="1724"/>
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            <begin position="248"/>
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    <sequence length="2296" mass="260205" checksum="C0C2D6BF161870B6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="3">Uncharacterized protein</fullName>
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        <name evidence="3" type="primary">LOC6897350</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="22"/>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033241622.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="13229" checksum="3208BC17ADD09DB2" modified="2020-08-12" version="1">MSSASLIVFCFLALLLGQTLGQDPAINYSLQWAKDTGINIRMIIEEKLPNTASAQAEGREVADTFDLGLTHCNAELLASRNVAQHKVCVNALAAGAYASLDRVAGQQWAIYGASSGASRIGLFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-05-03" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9C6XP39</accession>
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            <fullName evidence="5 18">NADH-ubiquinone oxidoreductase chain 2</fullName>
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        <name evidence="22" type="ORF">HCS66_mgp03</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="22"/>
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        <citation type="submission" date="2019-12" db="EMBL/GenBank/DDBJ databases">
            <title>Evolutionary conservation of topologically associating domains and their influences on genome synteny and structural variations in Drosophila.</title>
            <authorList>
                <person name="Liao Y."/>
                <person name="Chakraborty M."/>
                <person name="Zhang X."/>
                <person name="Emerson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">MV2-25</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
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        <citation type="submission" date="2020-03" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="NCBI Genome Project"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">MV2-25</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV2-25</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Mitochondrion inner membrane</location>
            <topology evidence="2 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the complex I subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="4 18"/>
    <dbReference type="RefSeq" id="YP_009742606.1">
        <property type="nucleotide sequence ID" value="NC_046603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9C6XP39"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Mitochondrion MT"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050175">
        <property type="entry name" value="Complex_I_Subunit_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010933">
        <property type="entry name" value="NADH_DH_su2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003917">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46552">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46552:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06444">
        <property type="entry name" value="NADH_dehy_S2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01436">
        <property type="entry name" value="NADHDHGNASE2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 18 22" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 18" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 18" id="KW-0520">NAD</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="14 18" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="19">
        <location>
            <begin position="24"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="NADH dehydrogenase subunit 2 C-terminal" evidence="20">
        <location>
            <begin position="286"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF06444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="YP_009742606.1"/>
        </source>
    </evidence>
    <sequence length="341" mass="39637" checksum="1E9B789C4C597EEB" modified="2023-05-03" version="1">MFNNSSKILFLTIMIIGTMITVTSNSWLGAWMGLEINLLSFIPLLSDNNNLTSTEASLKYFLTQALASTVLLFSSIILMLKNNMNIEINESFTSMIIMSALLLKMGAAPFHFWFPNMMEGLTWMNALMLMTWQKIAPLMLISYLNIKNLLLISVILSVIVGAIGGLNQTSLRKLMAFSSINHLGWMLMSLMISESVWLIYFMFYSFLSFILTFMFNIFKLFHLNQLFSWFMNSKVLKFTLFMNFLSLGGLPPFLGFLPKWLVIQELTFCNQYFTLTIMMMSTLITLFFYLRICYSAFMLNYYENNWTINTQFNNMNTNLYLICTFFSIFGLFLISLFFFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKG7</accession>
    <accession>A0A6I8UYJ7</accession>
    <name>B5DKG7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cytochrome c oxidase assembly factor 6 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6901850</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002133388.1">
        <property type="nucleotide sequence ID" value="XM_002133352.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKG7"/>
    <dbReference type="FunCoup" id="B5DKG7">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DKG7"/>
    <dbReference type="GeneID" id="6901850"/>
    <dbReference type="KEGG" id="dpo:6901850"/>
    <dbReference type="eggNOG" id="KOG3057">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142408_4_0_1"/>
    <dbReference type="InParanoid" id="B5DKG7"/>
    <dbReference type="OMA" id="ERAKCWS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244244">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DKG7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042775">
        <property type="term" value="P:mitochondrial ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008535">
        <property type="term" value="P:respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042289">
        <property type="entry name" value="COA6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46690">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46690:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133388.1"/>
        </source>
    </evidence>
    <sequence length="92" mass="10650" checksum="3F5D5E8BE50056EF" modified="2008-10-14" version="1">MGFPDKAERAKCWTTRDEYWKCLDASAPKHSSTSGEKVPNACKDLRKAFEQSCPGQWVKHFDRKRTYEQFKEKMATGYDPLEERATGEKTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>B5DL12</accession>
    <accession>A0A6I8UYR8</accession>
    <name>B5DL12_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L30-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6902050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133584.2">
        <property type="nucleotide sequence ID" value="XM_002133548.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DL12"/>
    <dbReference type="STRING" id="46245.B5DL12"/>
    <dbReference type="GeneID" id="6902050"/>
    <dbReference type="KEGG" id="dpo:6902050"/>
    <dbReference type="eggNOG" id="KOG2988">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2173611_0_0_1"/>
    <dbReference type="InParanoid" id="B5DL12"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244139">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="3">
        <location>
            <begin position="65"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133584.2"/>
        </source>
    </evidence>
    <sequence length="158" mass="17799" checksum="1F3487AB958168AE" modified="2016-01-20" version="2">MWQASAERKIMEYLRKRPETSTNMEKEISSVGNKKKFFCNKSIQENIRMTVKVNCDEVFCSHSCMMKAIREQKAKVVIVATKTPPSQKAEIEHFAKVAKCQIIYYGGRFIECANSCGKVFLFCTVAISTQKDAAIIRSLSEKLVAGIALFLDGKDVTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>B5DMA5</accession>
    <accession>A0A6I8UZD1</accession>
    <name>B5DMA5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-tubulin N-acetyltransferase</fullName>
            <shortName evidence="3">Alpha-TAT</shortName>
            <shortName evidence="3">TAT</shortName>
            <ecNumber evidence="3">2.3.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Acetyltransferase mec-17 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">LOC6901295</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-lysyl-[alpha-tubulin] + acetyl-CoA = N(6)-acetyl-L-lysyl-[alpha-tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15277"/>
            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.108"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase ATAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.108" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002133996.1">
        <property type="nucleotide sequence ID" value="XM_002133960.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041546.1">
        <property type="nucleotide sequence ID" value="XM_015186060.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DMA5"/>
    <dbReference type="SMR" id="B5DMA5"/>
    <dbReference type="GeneID" id="6901295"/>
    <dbReference type="KEGG" id="dpo:6901295"/>
    <dbReference type="eggNOG" id="KOG4601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025013_2_1_1"/>
    <dbReference type="OMA" id="FFIGRHP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249905">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DMA5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019799">
        <property type="term" value="F:tubulin N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03130">
        <property type="entry name" value="mec17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038746">
        <property type="entry name" value="Atat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007965">
        <property type="entry name" value="GNAT_ATAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327:SF0">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05301">
        <property type="entry name" value="Acetyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51730">
        <property type="entry name" value="GNAT_ATAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial for catalytic activity" evidence="3">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002133996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015041546.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="32104" checksum="9965204C682C5545" modified="2008-10-14" version="1">MTDFCFDIKPLFPEAIIKVSSDLLPQNFSGKRFMCADVTLKMAEVIDRMGQLSATAQDLINPVTTAQRLEKSDNQTVYLMADGKEGANGVVVGLLKVGTKDLYLYDETGQVHKVQRAPAVLDFYVHESRQRCGLGKELFEAMLAEEKWTPSKLSVDRPSQKLILFMARHYGLVRTIPQANHFVLYEGYFHGVSPSMPLLQATKSVPAILAAGSERQRTSAMTFHVGEANYRKRRLQDPQQQSCSTTDLLSSRCNSDCSMAEIIQGSRARGTNASHAADGQISRTNVRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>B5DP06</accession>
    <accession>A0A6I8V082</accession>
    <name>B5DP06_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="12 13">3.2.1.106</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">GCS1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="12 13"/>
    <dbReference type="RefSeq" id="XP_002134631.1">
        <property type="nucleotide sequence ID" value="XM_002134595.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DP06"/>
    <dbReference type="SMR" id="B5DP06"/>
    <dbReference type="FunCoup" id="B5DP06">
        <property type="interactions" value="1411"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DP06"/>
    <dbReference type="GeneID" id="6901002"/>
    <dbReference type="KEGG" id="dpo:6901002"/>
    <dbReference type="eggNOG" id="KOG2161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007380_1_0_1"/>
    <dbReference type="InParanoid" id="B5DP06"/>
    <dbReference type="OMA" id="FNWYNTT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249286">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000009">
        <property type="entry name" value="mannosyl-oligosaccharide glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.110">
        <property type="entry name" value="Glycosyl hydrolase family 63, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031631">
        <property type="entry name" value="Glyco_hydro_63N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038518">
        <property type="entry name" value="Glyco_hydro_63N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF11">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16923">
        <property type="entry name" value="Glyco_hydro_63N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 N-terminal" evidence="16">
        <location>
            <begin position="101"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="15">
        <location>
            <begin position="351"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002134631.1"/>
        </source>
    </evidence>
    <sequence length="843" mass="95969" checksum="3F02F2F1E7D1F166" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>B5DR88</accession>
    <accession>A0A6I8V260</accession>
    <name>B5DR88_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lysozyme</fullName>
            <ecNumber evidence="3">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6899953</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 22 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002135321.1">
        <property type="nucleotide sequence ID" value="XM_002135285.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DR88"/>
    <dbReference type="SMR" id="B5DR88"/>
    <dbReference type="FunCoup" id="B5DR88">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="GeneID" id="6899953"/>
    <dbReference type="KEGG" id="dpo:6899953"/>
    <dbReference type="eggNOG" id="ENOG502S1S1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111620_2_1_1"/>
    <dbReference type="InParanoid" id="B5DR88"/>
    <dbReference type="OMA" id="TIQCELA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249765">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16899">
        <property type="entry name" value="LYZ_C_invert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000001">
        <property type="entry name" value="Lysozyme C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001916">
        <property type="entry name" value="Glyco_hydro_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019799">
        <property type="entry name" value="Glyco_hydro_22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000974">
        <property type="entry name" value="Glyco_hydro_22_lys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407:SF36">
        <property type="entry name" value="GEO02684P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00062">
        <property type="entry name" value="Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00137">
        <property type="entry name" value="LYSOZYME"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00135">
        <property type="entry name" value="LYZLACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00263">
        <property type="entry name" value="LYZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00128">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51348">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="5" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycosyl hydrolases family 22 (GH22)" evidence="11">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002135321.1"/>
        </source>
    </evidence>
    <sequence length="140" mass="15762" checksum="2D4C33BC679A0C62" modified="2008-10-14" version="1">MKAFIVLVALVLAAPAFGRTMDRCSLAREMSDLGVPRDQLDKWTCIAEHESSYRTGVVGPENYNGSNDYGIFQINDYYWCAPPSGRFSYNECGLSCNALLTDNIEHSVRCAQKVLSQQGWSAWSTWHYCSGWLPSIDECF</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>B5DRH8</accession>
    <accession>A0A6I8V1G8</accession>
    <name>B5DRH8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YTH domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Ythdc1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002135520.1">
        <property type="nucleotide sequence ID" value="XM_002135484.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DRH8"/>
    <dbReference type="SMR" id="B5DRH8"/>
    <dbReference type="FunCoup" id="B5DRH8">
        <property type="interactions" value="681"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DRH8"/>
    <dbReference type="GeneID" id="6900181"/>
    <dbReference type="KEGG" id="dpo:6900181"/>
    <dbReference type="eggNOG" id="KOG1902">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388961_0_0_1"/>
    <dbReference type="InParanoid" id="B5DRH8"/>
    <dbReference type="OMA" id="TLPMREM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249640">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000002">
        <property type="entry name" value="YTH domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF3">
        <property type="entry name" value="YTH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="7">
        <location>
            <begin position="260"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="430"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="593"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="660"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="441"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="599"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="660"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="697"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002135520.1"/>
        </source>
    </evidence>
    <sequence length="735" mass="77756" checksum="57BB95A0D95AEDC4" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1989-10-01" modified="2025-06-18" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>P12348</accession>
    <accession>Q29I05</accession>
    <name>PER_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Period circadian protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">per</name>
        <name type="ORF">GA15408</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1988" name="EMBO J." volume="7" first="3929" last="3937">
            <title>Interspecific comparison of the period gene of Drosophila reveals large blocks of non-conserved coding DNA.</title>
            <authorList>
                <person name="Colot H.V."/>
                <person name="Hall J.C."/>
                <person name="Rosbash M."/>
            </authorList>
            <dbReference type="PubMed" id="3208754"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>Ayala</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for biological clock functions. Determines the period length of circadian and ultradian rhythms; an increase in PER dosage leads to shortened circadian rhythms and a decrease leads to lengthened circadian rhythms. Essential for the circadian rhythmicity of locomotor activity, eclosion behavior, and for the rhythmic component of the male courtship song that originates in the thoracic nervous system. The biological cycle depends on the rhythmic formation and nuclear localization of the TIM-PER complex. Light induces the degradation of TIM, which promotes elimination of PER. Nuclear activity of the heterodimer coordinatively regulates PER and TIM transcription through a negative feedback loop. Behaves as a negative element in circadian transcriptional loop. Does not appear to bind DNA, suggesting indirect transcriptional inhibition.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterodimer with timeless (TIM); the complex then translocates into the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
        <text evidence="1">Nuclear at specific periods of the day. First accumulates in the perinuclear region about one hour before translocation into the nucleus. Interaction with Tim is required for nuclear localization (By similarity).</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The run of Gly-Thr is implicated in the maintenance of circadian period at different temperatures. Deletion of the repeat leads to a shortening of the courtship song cycle period, and thus could be important for determining features of species-specific mating behavior.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated with a circadian rhythmicity, probably by the double-time protein (dbt). Phosphorylation could be implicated in the stability of per monomer and in the formation of heterodimer per-tim.</text>
    </comment>
    <dbReference type="EMBL" id="X13878">
        <property type="protein sequence ID" value="CAA32082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EAL31601.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S01827">
        <property type="entry name" value="S01827"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P12348"/>
    <dbReference type="SMR" id="P12348"/>
    <dbReference type="FunCoup" id="P12348">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="46245.P12348"/>
    <dbReference type="eggNOG" id="KOG3753">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002704_0_0_1"/>
    <dbReference type="InParanoid" id="P12348"/>
    <dbReference type="OMA" id="SYPSCTQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001222">
        <property type="term" value="F:transcription corepressor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.770:FF:000001">
        <property type="entry name" value="Period circadian protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000066">
        <property type="entry name" value="Period circadian protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000072">
        <property type="entry name" value="Period circadian protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.770">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050760">
        <property type="entry name" value="Period_circadian_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269:SF16">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0090">Biological rhythms</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000162602" description="Period circadian protein">
        <location>
            <begin position="1"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="domain" description="PAS 1" evidence="3">
        <location>
            <begin position="189"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PAS 2" evidence="3">
        <location>
            <begin position="339"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="657"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="659"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="repeat" description="3">
        <location>
            <begin position="661"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="repeat" description="4; approximate">
        <location>
            <begin position="663"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="repeat" description="5">
        <location>
            <begin position="665"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="repeat" description="6">
        <location>
            <begin position="667"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="repeat" description="7">
        <location>
            <begin position="674"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="8">
        <location>
            <begin position="676"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="596"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="8 X 2 AA approximate tandem repeats of G-T">
        <location>
            <begin position="657"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="746"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="928"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1014"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1143"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1227"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nuclear localization signal" evidence="2">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="746"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="781"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="805"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="831"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="864"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="885"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="903"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1014"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1025"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1143"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1257"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <original>P</original>
        <variation>S</variation>
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <original>D</original>
        <variation>E</variation>
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <original>EL</original>
        <variation>DV</variation>
        <location>
            <begin position="537"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <location>
            <begin position="726"/>
            <end position="730"/>
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    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <original>G</original>
        <variation>D</variation>
        <location>
            <position position="763"/>
        </location>
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    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <location>
            <begin position="777"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <original>PDS</original>
        <variation>ADA</variation>
        <location>
            <begin position="873"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <original>T</original>
        <variation>A</variation>
        <location>
            <position position="1061"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <original>S</original>
        <variation>N</variation>
        <location>
            <position position="1104"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <location>
            <begin position="1117"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <original>A</original>
        <variation>T</variation>
        <location>
            <position position="1165"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA32082." evidence="5" ref="1">
        <original>S</original>
        <variation>P</variation>
        <location>
            <position position="1179"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="1271" mass="135420" checksum="4B2B0D88A9FE7495" modified="2008-12-16" version="3"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29BX8</accession>
    <name>CISD2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>CDGSH iron-sulfur domain-containing protein 2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Cisd2</name>
        <name type="ORF">GA13095</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CISD protein family. CISD2 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="EAL26868.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001357734.2">
        <property type="nucleotide sequence ID" value="XM_001357697.3"/>
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        <property type="interactions" value="1435"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29BX8"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0284743">
        <property type="protein sequence ID" value="FBpp0283181"/>
        <property type="gene ID" value="FBgn0073133"/>
    </dbReference>
    <dbReference type="GeneID" id="4800463"/>
    <dbReference type="KEGG" id="dpo:4800463"/>
    <dbReference type="CTD" id="493856"/>
    <dbReference type="eggNOG" id="KOG3461">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132293_1_0_1"/>
    <dbReference type="InParanoid" id="Q29BX8"/>
    <dbReference type="OMA" id="QIRKHEP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073133">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="entry name" value="CDGSH iron-sulfur domain, mitoNEET-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045131">
        <property type="entry name" value="CISD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018967">
        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019610">
        <property type="entry name" value="FeS-contain_mitoNEET_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13680">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13680:SF5">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10660">
        <property type="entry name" value="MitoNEET_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09360">
        <property type="entry name" value="zf-CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000316011" description="CDGSH iron-sulfur domain-containing protein 2 homolog">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="3">
        <location>
            <begin position="59"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9VAM6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="132" mass="14407" checksum="0AE1A356B5E79A73" modified="2009-01-20" version="2">MEPISHVVKSSLPNYLSSLPIPDSFGGWFKLSFKDWLALIPPTVVVAGLGYTTYLAFCPAARCAGKDSGRCNSSIRKNEAKVVTMVDVEDIAGQAAFCRCWKTKNWPYCDGSHGEHNKQTGDNVGPVVVKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D42</accession>
    <accession>A0A6I8UBZ2</accession>
    <name>Q29D42_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">UPF0046 protein C25E10.12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">LOC4812688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
            <tissue evidence="4 5 6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0046 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353071.2">
        <property type="nucleotide sequence ID" value="XM_001353035.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044129.1">
        <property type="nucleotide sequence ID" value="XM_015188643.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237449.1">
        <property type="nucleotide sequence ID" value="XM_033381558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D42"/>
    <dbReference type="SMR" id="Q29D42"/>
    <dbReference type="STRING" id="46245.Q29D42"/>
    <dbReference type="GeneID" id="4812688"/>
    <dbReference type="KEGG" id="dpo:4812688"/>
    <dbReference type="eggNOG" id="KOG3947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041441_1_0_1"/>
    <dbReference type="OMA" id="IKTRICM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074131">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07379">
        <property type="entry name" value="MPP_239FB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024201">
        <property type="entry name" value="Calcineurin-like_Pesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051693">
        <property type="entry name" value="UPF0046_metallophosphoest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905:SF0">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035808">
        <property type="entry name" value="Pdiesterase_Brain_239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="46"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353071.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015044129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033237449.1"/>
        </source>
    </evidence>
    <sequence length="303" mass="33552" checksum="F30E4EB783DEE57B" modified="2008-10-14" version="2">MEIPTVPVHPLSSDPTAAWKDISKTQRVIKVTMKPPTNAVAANKARVVCMSDTHSLTPYIKFDVPDGDIFIHAGDFTKCGQLEEVEEFNTWIGALPHRHKIVIAGNHELSFDRTFTHPFQNNAKGHSSTKHTGMSILDDLPTLGNAKESMESAVQTQNIREVLTNCTYLEDQLLELWGVRIYGSPWQPEFCRWAFNVPRGAACLEKWNQVPAGVDILVTHTPPVGHGDLCCSGVRAGCVELLSTVQQRVKPKYHVFGHVHEGYGITSDGRIIYVNASTCDINYLPNNPPIVFDVTLPPGMSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DP9</accession>
    <accession>A0A6I8UBM0</accession>
    <name>Q29DP9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import receptor subunit TOM5 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4812267</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352864.1">
        <property type="nucleotide sequence ID" value="XM_001352828.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DP9"/>
    <dbReference type="GeneID" id="4812267"/>
    <dbReference type="KEGG" id="dpo:4812267"/>
    <dbReference type="HOGENOM" id="CLU_3144383_0_0_1"/>
    <dbReference type="InParanoid" id="Q29DP9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076815">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DP9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019603">
        <property type="entry name" value="Tom5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10642">
        <property type="entry name" value="Tom5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001352864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="49" mass="5517" checksum="61B0C07DB5849208" modified="2006-04-04" version="1">MFRLQQAQPDPAEEQKRVASEVRFSFIIFGTLCAAIRLAPIVLKQLNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DV6</accession>
    <accession>A0A6I8UBE3</accession>
    <name>Q29DV6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhythmically expressed gene 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Reg-2</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352807.1">
        <property type="nucleotide sequence ID" value="XM_001352771.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DV6"/>
    <dbReference type="SMR" id="Q29DV6"/>
    <dbReference type="FunCoup" id="Q29DV6">
        <property type="interactions" value="534"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DV6"/>
    <dbReference type="GeneID" id="4812407"/>
    <dbReference type="KEGG" id="dpo:4812407"/>
    <dbReference type="eggNOG" id="KOG3085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_0_1"/>
    <dbReference type="InParanoid" id="Q29DV6"/>
    <dbReference type="OMA" id="WWRQLIA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076619">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16415">
        <property type="entry name" value="HAD_dREG-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.720">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011949">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044924">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02252">
        <property type="entry name" value="DREG-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001352807.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="30035" checksum="A1EA382EC719B3B5" modified="2006-04-04" version="1">MRSLNRFRLITFDVTNTLLQFRTSPGKQYGEIGALFGARCDNNELAKNFKANWYKMNRDYPNFGRDTKPPLEWQQWWRQLIAGTFSESGAAIPTEKLTNFSNHLLELYKTSICWQPCNGSVELLQHLRKELKPNKCKLGVIANFDPRLPALLQNTKLDQYLDFALTSYEAQAEKPDPLIFQRAMEESGLKHLKPEECLHVGDGPTTDYLAAKELGWHAALVHEKSYSYLMKKYGEDIDRDHVFPSLYDFHKKISDGAVVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FW8</accession>
    <accession>A0A6I8UEX7</accession>
    <name>Q29FW8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chitinase-like protein Idgf4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Idgf4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family. IDGF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354408.3">
        <property type="nucleotide sequence ID" value="XM_001354372.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FW8"/>
    <dbReference type="SMR" id="Q29FW8"/>
    <dbReference type="FunCoup" id="Q29FW8">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="GeneID" id="4814222"/>
    <dbReference type="KEGG" id="dpo:4814222"/>
    <dbReference type="eggNOG" id="KOG2806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002833_3_2_1"/>
    <dbReference type="InParanoid" id="Q29FW8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074698">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FW8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02873">
        <property type="entry name" value="GH18_IDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000007">
        <property type="entry name" value="chitinase-like protein Idgf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000071">
        <property type="entry name" value="Imaginal disc growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015520">
        <property type="entry name" value="IDGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF235">
        <property type="entry name" value="CHITINASE-LIKE PROTEIN IDGF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354408.3"/>
        </source>
    </evidence>
    <sequence length="443" mass="48714" checksum="9E220405843E2487" modified="2016-01-20" version="3">MKLYALLSLLVGSLAIGQISAAAGSHHLLCYYDGSSFVREGLSKLTLNDLDPALQYCTHLIYGYAGINPSSNKLVSTNEKLDLDLGSSLFRQVTSLKRKYPALKVLLSVGGDKDIVDPENNKYLTLLESSNARIPFINSAHSLVKTYGFDGLDLAWQFPKNKPKKVHGSIGKFWKGFKKIFSGDHIVDEKSEEHKEEFTALVRELKNAFRPDGYLLGLSVLPNVNSSLFFDVPAVINNLDYVNLHAYDFQTPERNKEVADFPAPIYEANERNPELNVNYQVKYWVNNRAPASKINVAIAAYGRAWKLTKDSGLTGLPPVAETDGVAPAGLQTQVDGLLSWPEVCAKLPNPANQHLKGADGPLRKVGDPTKRFGSYAYRSADDAGENGVWVGYEDPDTAGIKAEYVKREGLGGVALVDLSYDDFRGGCTGHDKFPILRTIKSKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-12-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HB2</accession>
    <name>MMSA_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], mitochondrial</fullName>
            <shortName evidence="3">MMSDH</shortName>
            <ecNumber evidence="3">1.2.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Malonate-semialdehyde dehydrogenase [acylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GA14712</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Probable malonate and methylmalonate semialdehyde dehydrogenase involved in the catabolism of valine, thymine, and compounds catabolized by way of beta-alanine, including uracil and cytidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-methyl-3-oxopropanoate + NAD(+) + CoA + H2O = propanoyl-CoA + hydrogencarbonate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20804"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:20805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-oxopropanoate + NAD(+) + CoA + H2O = hydrogencarbonate + acetyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76615"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:76616"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="sequence caution" evidence="6">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL31846" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="3"/>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EAL31846.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HB2"/>
    <dbReference type="SMR" id="Q29HB2"/>
    <dbReference type="FunCoup" id="Q29HB2">
        <property type="interactions" value="1297"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HB2"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0284054">
        <property type="protein sequence ID" value="FBpp0282492"/>
        <property type="gene ID" value="FBgn0074739"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815188"/>
    <dbReference type="eggNOG" id="KOG2449">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_10_1"/>
    <dbReference type="InParanoid" id="Q29HB2"/>
    <dbReference type="OMA" id="GGAKNHI"/>
    <dbReference type="PhylomeDB" id="Q29HB2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074739">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019859">
        <property type="term" value="P:thymine metabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006573">
        <property type="term" value="P:valine metabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="4">
        <location>
            <begin position="1"/>
            <end status="unknown"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000312824" description="Probable methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], mitochondrial">
        <location>
            <begin status="unknown"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2 5">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P42412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q02253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="EAL31846.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="56064" checksum="E8336961D816B35D" modified="2006-04-04" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q29II9</accession>
    <accession>A0A6I8VH71</accession>
    <name>Q29II9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">ND-B16.6</name>
        <name evidence="16" type="synonym">LOC4815861</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17">MV-25-SWS-2005</strain>
            <strain evidence="16">MV2-25</strain>
            <tissue evidence="16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Complex I functions in the transfer of electrons from NADH to the respiratory chain. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Complex I is composed of 45 different subunits. Interacts with CARD15, but not with CARD4. Interacts with STAT3, but not with STAT1, STAT2 and STAT5A. Interacts with OLFM4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Single-pass membrane protein</topology>
            <orientation evidence="1 14">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the complex I NDUFA13 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355605.1">
        <property type="nucleotide sequence ID" value="XM_001355569.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041291.1">
        <property type="nucleotide sequence ID" value="XM_015185805.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29II9"/>
    <dbReference type="SMR" id="Q29II9"/>
    <dbReference type="STRING" id="46245.Q29II9"/>
    <dbReference type="GeneID" id="4815861"/>
    <dbReference type="KEGG" id="dpo:4815861"/>
    <dbReference type="eggNOG" id="KOG3300">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119720_0_1_1"/>
    <dbReference type="OMA" id="YGIREQH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077468">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29II9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009346">
        <property type="entry name" value="GRIM-19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12966:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12966">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE 1 ALPHA SUBCOMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06212">
        <property type="entry name" value="GRIM-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001355605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015041291.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17748" checksum="430614502BCBAFCE" modified="2006-04-04" version="1">MATAVPHVPPKQDLPPAGGYKKIPFARVPPKSYFTGFTMIGTYVAVTTVGLGIYYLTAKKVKRDEIEMRSAQNVIFPILIAERDREFLRQLRRNRDEEAELMKNVPGWEVGTWYGEPVFKTLPEDTLVTPIFKEFYAHADWKSYAKRAHIKLWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IP9</accession>
    <accession>A0A6I8UHD1</accession>
    <name>Q29IP9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rabphilin-3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Rph</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355545.2">
        <property type="nucleotide sequence ID" value="XM_001355509.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IP9"/>
    <dbReference type="SMR" id="Q29IP9"/>
    <dbReference type="FunCoup" id="Q29IP9">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IP9"/>
    <dbReference type="GeneID" id="4815985"/>
    <dbReference type="KEGG" id="dpo:4815985"/>
    <dbReference type="eggNOG" id="KOG1013">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011461_1_0_1"/>
    <dbReference type="InParanoid" id="Q29IP9"/>
    <dbReference type="OMA" id="IRYHTSE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071119">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IP9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017158">
        <property type="term" value="P:regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061669">
        <property type="term" value="P:spontaneous neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04035">
        <property type="entry name" value="C2A_Rabphilin_Doc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08384">
        <property type="entry name" value="C2B_Rabphilin_Doc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000236">
        <property type="entry name" value="Rabphilin, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000244">
        <property type="entry name" value="Rabphilin, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000182">
        <property type="entry name" value="rabphilin-3A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043566">
        <property type="entry name" value="Rabphilin/DOC2/Noc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047022">
        <property type="entry name" value="Rabphilin_Doc2_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729:SF6">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="162"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="315"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355545.2"/>
        </source>
    </evidence>
    <sequence length="642" mass="72192" checksum="447CC094A29D4AD3" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IZ5</accession>
    <accession>A0A6I8UH78</accession>
    <name>Q29IZ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aurora kinase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">png</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355449.2">
        <property type="nucleotide sequence ID" value="XM_001355413.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IZ5"/>
    <dbReference type="SMR" id="Q29IZ5"/>
    <dbReference type="FunCoup" id="Q29IZ5">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IZ5"/>
    <dbReference type="GeneID" id="4815832"/>
    <dbReference type="KEGG" id="dpo:4815832"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="Q29IZ5"/>
    <dbReference type="OMA" id="RIPYDYK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071050">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IZ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="8"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001355449.2"/>
        </source>
    </evidence>
    <sequence length="287" mass="32956" checksum="77D17550A4438861" modified="2008-10-14" version="2">MNLGDPMLRAVRVLGQGTFGRVFLCLQHEGSRQQRQVCVKRIIVRNPKTELSLIKEEIYIISQLRHPNIVEFIRSFNHSGTVNIVMEYAPNGTLRDVIQHTANLPHERLLRYFGDMVIGLEYLHIRNVIHRDIKPENMLLDAGDRVKIADFGISNVHAPSKQHLAGVGTPLYMAPEVMSGNGKVDYKSDIWSLGLVLYELCVGRSPFVALIDPGATPQQVHTVVVTLTRPKLDCQLIRRLHNSTWSRICELMVVYEQERRICVPDIFRLDPCITSFLYKQYFNYNYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q29M25</accession>
    <accession>A0A6I8UK86</accession>
    <name>Q29M25_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Osi21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Osi21</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356804.1">
        <property type="nucleotide sequence ID" value="XM_001356768.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29M25"/>
    <dbReference type="FunCoup" id="Q29M25">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29M25"/>
    <dbReference type="GeneID" id="4817029"/>
    <dbReference type="KEGG" id="dpo:4817029"/>
    <dbReference type="eggNOG" id="ENOG502SZN6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085501_0_0_1"/>
    <dbReference type="InParanoid" id="Q29M25"/>
    <dbReference type="OMA" id="DWGSAWG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012464">
        <property type="entry name" value="DUF1676"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879">
        <property type="entry name" value="FI03362P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879:SF15">
        <property type="entry name" value="OSIRIS 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07898">
        <property type="entry name" value="DUF1676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356804.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="30407" checksum="AEFA0B05338F75BE" modified="2006-04-04" version="1">MKRFEWLMILACIASSTAAATQRRRRHSIVEAAPTTASNSDWSLGLGLGPEMSLVRRVYDDCQDKNDFIGCLKQKALHALSRALDQESIKIMDGLVLEKQNQTDTESILGSLTDARQFGSLSPIDRALLAKADKLLRTHTLKLDMDMGMGKESGMAVGRGHDKKKKHKDGGHIKYVIAALLTAMGIAGPLGLKALAAIAGKALVISKVALTIAGIIALKKLFSHDHSEETSFQVHAGEHNRRNTYVIRPVSKTNAGMGVTAAGGSSSVDPYRYYYESQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NJ8</accession>
    <accession>A0A6I8UL06</accession>
    <name>Q29NJ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PI-PLC X domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PLCXD</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357154.2">
        <property type="nucleotide sequence ID" value="XM_001357118.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NJ8"/>
    <dbReference type="SMR" id="Q29NJ8"/>
    <dbReference type="FunCoup" id="Q29NJ8">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="GeneID" id="4817720"/>
    <dbReference type="KEGG" id="dpo:4817720"/>
    <dbReference type="eggNOG" id="KOG4306">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039880_0_0_1"/>
    <dbReference type="InParanoid" id="Q29NJ8"/>
    <dbReference type="OMA" id="WFTTGVP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073409">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29NJ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08622">
        <property type="entry name" value="PI-PLCXDc_CG14945_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF149">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C X DOMAIN CONTAINING, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="213"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357154.2"/>
        </source>
    </evidence>
    <sequence length="485" mass="55214" checksum="0A3E487591D53D26" modified="2008-10-14" version="2">MFLQTIIFILALTGPATAGLPEVQTIKAARTDDDGNRLKLWLTINARKRFLEISWKNAPARMGDNVLLTVQDPHSFELRTLPTGTPLASFASDEGSGSGDGAADYVPVQGFTRKPLTSLAKSTKPPSSRQYWVANEGTAEIVAAIQPSLSTQWFTTGVPFDYALSRNVTAQTFCYGYWASYINAEGRILAKTCVRAYPRWMNELRSVVGELRLRDLFIPGTHDSGSYRPNFDPLIRESLVTKYALTQDDDIMGQLMHGVRYLDIRVGYYRASQEKFFIYHGITKQRPLQEVINQVKDFVHETNEIVIFGLKEFPVGFGKSLGVHRLLVSYLREQFQDLIAHPSLTWRASLLDIWSRQQNVILAYDKHAIVEEFPQLLFGAVEQRWGNVQTWPRLETYLRNINDFDVSRFSSRLVADMAELTPETWDVILDKHGGLRKMADSINWRISQLYRDELGENSNIVAVDFIRGTTIVETAIDWNRRKIAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NZ9</accession>
    <accession>A0A6I8V7K6</accession>
    <name>Q29NZ9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exportin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">emb</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035518.1">
        <property type="nucleotide sequence ID" value="XM_015180032.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NZ9"/>
    <dbReference type="SMR" id="Q29NZ9"/>
    <dbReference type="FunCoup" id="Q29NZ9">
        <property type="interactions" value="3533"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NZ9"/>
    <dbReference type="GeneID" id="4818039"/>
    <dbReference type="KEGG" id="dpo:4818039"/>
    <dbReference type="eggNOG" id="KOG2020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011906_0_0_1"/>
    <dbReference type="InParanoid" id="Q29NZ9"/>
    <dbReference type="OMA" id="WAFKHNN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072292">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29NZ9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001255">
        <property type="entry name" value="Exportin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="42"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015035518.1"/>
        </source>
    </evidence>
    <sequence length="1062" mass="122696" checksum="4DD71DFD7DCD2E69" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PM1</accession>
    <accession>A0A6I8ULB4</accession>
    <name>Q29PM1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">sphingolipid 4-desaturase</fullName>
            <ecNumber evidence="3">1.14.19.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ifc</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the fatty acid desaturase type 1 family. DEGS subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.17" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001357203.1">
        <property type="nucleotide sequence ID" value="XM_001357167.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PM1"/>
    <dbReference type="FunCoup" id="Q29PM1">
        <property type="interactions" value="966"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29PM1"/>
    <dbReference type="GeneID" id="4818051"/>
    <dbReference type="KEGG" id="dpo:4818051"/>
    <dbReference type="eggNOG" id="KOG2987">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032156_0_0_1"/>
    <dbReference type="InParanoid" id="Q29PM1"/>
    <dbReference type="OMA" id="KEFYETM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081510">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29PM1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03508">
        <property type="entry name" value="Delta4-sphingolipid-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011388">
        <property type="entry name" value="DES1/DES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF8">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017228">
        <property type="entry name" value="Sphnglp_dlt4_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="10">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001357203.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="37196" checksum="2426A249C0827920" modified="2006-04-04" version="1">MGQKVSRTDFEWVYTEEPHASRRKIILEKYPQIKKLFGHDPNFKWIAGAMVLTQILALFVVKDLSWSWLIVAAYCFGGIINHSLMLAVHEISHNLAFGHSRPMHNRILGFICNLPIGLPMSISFKKYHLEHHRYQGDEAIDTDIPTLLEARLFDTTFGKFLWVCLQPFFYIFRPLVINPKPPTRLEIINTIVQLTFNALIVYFLGWKPLAYLLIGSILAMGLHPVAGHFISEHYMFAKGFETYSYYGPLNWITFNVGYHNEHHDFPAVPGSRLPEVKRIAPEFYETMPQHTSWSRVLYDFIMDPAVGPYARVKRRQRGLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0E0</accession>
    <accession>A0A6I8UCY3</accession>
    <name>Q2M0E0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Grx1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer; active form. Homodimer; inactive form. The homodimer is probably linked by 1 2Fe-2S cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353483.3">
        <property type="nucleotide sequence ID" value="XM_001353447.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0E0"/>
    <dbReference type="SMR" id="Q2M0E0"/>
    <dbReference type="FunCoup" id="Q2M0E0">
        <property type="interactions" value="769"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0E0"/>
    <dbReference type="GeneID" id="4813783"/>
    <dbReference type="KEGG" id="dpo:4813783"/>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_7_2_1"/>
    <dbReference type="InParanoid" id="Q2M0E0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079902">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0E0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000026">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF5">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="7">
        <location>
            <begin position="74"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353483.3"/>
        </source>
    </evidence>
    <sequence length="156" mass="17247" checksum="13A71D795232BF82" modified="2016-01-20" version="3">MLSIIVRCLPIAHQRLSNQRSSPQSLSTQPVKFPFPLLDFDHMGSIGSSLRSQTIDMSSKSAKFVENTIASHKVVIFSKTFCPYCKMAKEPFQKLNVQATVIELDGNPDGDEIQTVLGTITGARTVPRVFIDGKFVGGGTDIKRMYDTGDLQKYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NRQ1</accession>
    <accession>A0A6I8V7U5</accession>
    <name>A0A0R3NRQ1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18 19 20" type="primary">Sam-S</name>
    </gene>
    <organism evidence="16 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17 18 19">MV-25-SWS-2005</strain>
            <tissue evidence="17 18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="RefSeq" id="XP_015035612.1">
        <property type="nucleotide sequence ID" value="XM_015180126.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035614.1">
        <property type="nucleotide sequence ID" value="XM_015180128.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035615.1">
        <property type="nucleotide sequence ID" value="XM_015180129.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035617.1">
        <property type="nucleotide sequence ID" value="XM_015180131.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NRQ1"/>
    <dbReference type="SMR" id="A0A0R3NRQ1"/>
    <dbReference type="STRING" id="46245.A0A0R3NRQ1"/>
    <dbReference type="GeneID" id="4817790"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075438">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NRQ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="29"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="140"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="15">
        <location>
            <begin position="263"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015035612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015035614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015035615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015035617.1"/>
        </source>
    </evidence>
    <sequence length="407" mass="44755" checksum="764FDDF1FFA02713" modified="2016-01-20" version="1">MPQKTNGHSANGCNGSNGNSYDMEDGQTFLFTSESVGEGHPDKMCDQISDAILDAHLKQDPNAKVACETVAKTGMILLCGEITSKAVVDYQKVVRETVQHIGYDDSSKGFDFKTCNVLLALDQQSPEIAAGVHVNRPEEEIGAGDQGIMFGYATDETDECMPLTVVLAHKLNEKIAELRRSDVFWWARPDSKTQVTCEYLFNQGSAVPKRVHTIVVSMQHSEKISLETLRSEVMEKVVKVVIPAKYFDANTIVHINPCGLFVIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFTKVDRSAAYAARWVAKSLVKAGLCKRCLVQVSYAIGLAEPLSITVFDYGTSHKSQKELLEIIKRNFDLRPGKIVKDLNLRQPIYQRTSTYGHFGRDGFSWEEAKHLEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NSH0</accession>
    <accession>A0A6I8V8K7</accession>
    <name>A0A0R3NSH0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC26532517</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036084.1">
        <property type="nucleotide sequence ID" value="XM_015180598.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NSH0"/>
    <dbReference type="GeneID" id="26532517"/>
    <dbReference type="KEGG" id="dpo:26532517"/>
    <dbReference type="InParanoid" id="A0A0R3NSH0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271880">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NSH0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="THAP-type" evidence="5">
        <location>
            <begin position="28"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015036084.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="27464" checksum="02D791E8E9FEDCD6" modified="2016-01-20" version="1">MENNPFEMEWVRPIVKCLVKGCSSHERTYFVHKGNRQHWFANLRISGAPTRAKVCVKHFLDECFVNGKLRIGSLPTENLGHEPLHRPVFSRWQKRYRRRIKPELKTESDITVKPPLSESLNIENSLLSAESLARLEQAADAEFEKYIVEDEMSDGGEQTTHSESHDNDRFIIEDQLSDSMEEASHSQSESSLILFLQQRNNELQAEAECLRVENTQLRMENISLKKSFEIHKCFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NYH7</accession>
    <accession>A0A6I8W9N9</accession>
    <name>A0A0R3NYH7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Flotillin-2 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Flo2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the band 7/mec-2 family. Flotillin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240103.1">
        <property type="nucleotide sequence ID" value="XM_033384212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NYH7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244429">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NYH7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016600">
        <property type="term" value="C:flotillin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045661">
        <property type="term" value="P:regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03399">
        <property type="entry name" value="SPFH_flotillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000003">
        <property type="entry name" value="Flotillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027705">
        <property type="entry name" value="Flotillin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806:SF46">
        <property type="entry name" value="FLOTILLIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806">
        <property type="entry name" value="FLOTILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="5">
        <location>
            <begin position="97"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033240103.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="47878" checksum="CB8E10F1071E034D" modified="2016-01-20" version="1">MGNIHTTGPNEALIVSGGCCGSTKKRTIVGGWAWAWWLVTDVQRLSLNVMTLNPMCENVETAQGVPLTVTGVAQCKIMKMMNVYYFHHQADELLGTASEQFLGKSVKEIKQTILQTLEGHLRAILGTLTVEEVYKDRDQFAALVREVAAPDVGRMGIEILSFTIKDVYDDVQYLASLGKAQTAVVKRDADAGVAEANRDAGIREAECEKSAMDVKYSTDTKIEDNTRMYKLQKANFDQEINTAKAESQLAYELQAAKIRQRIRNEEIQIEVVERRKQIEIESQEVQRKDKELTGTVKLPAEAEAFRLQTLAQAKQCQTIEGARAEAERIRKIGAAEAHAIELVGKAEAERMRMKAHVYKQYGDAAIMNIVLESLPKIAAEVAAPLAKTEEIVLIGGNDNVTNDVTRLVAQLPPSINALTGVDLSKVLAKIPGAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P935</accession>
    <accession>A0A6I8WA88</accession>
    <name>A0A0R3P935_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6899999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240207.1">
        <property type="nucleotide sequence ID" value="XM_033384316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P935"/>
    <dbReference type="KEGG" id="dpo:6899999"/>
    <dbReference type="InParanoid" id="A0A0R3P935"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033240207.1"/>
        </source>
    </evidence>
    <sequence length="178" mass="20589" checksum="9C50A1743B7E0F3E" modified="2016-01-20" version="1">MDGLLCVWILLGALTTTHAHVTFTNLKCEMADRTFGDVMCRIKAVNRTHKYMELRAQLFKLPVENITVNLKLMRYNHGYKVFFIDVNVDACKFLANPRNPIVRSFYNIFKKNSNANHTCPYNHDVIVDKLFTGNLEADFAKYVPMVNGDYAFFTEWSTNKIHRAFLKVYFSLSGSKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBP8</accession>
    <name>A0A6I8UBP8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Est-Q</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001352976.2">
        <property type="nucleotide sequence ID" value="XM_001352940.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBP8"/>
    <dbReference type="FunCoup" id="A0A6I8UBP8">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812620"/>
    <dbReference type="InParanoid" id="A0A6I8UBP8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026370983" description="Carboxylic ester hydrolase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="42"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001352976.2"/>
        </source>
    </evidence>
    <sequence length="559" mass="61503" checksum="96C412113B5AF44B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBX6</accession>
    <name>A0A6I8UBX6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">VhaM9.7-a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353056.3">
        <property type="nucleotide sequence ID" value="XM_001353020.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBX6"/>
    <dbReference type="FunCoup" id="A0A6I8UBX6">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812672"/>
    <dbReference type="InParanoid" id="A0A6I8UBX6"/>
    <dbReference type="OMA" id="VIIMRHE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071802">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBX6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033181">
        <property type="term" value="C:plasma membrane proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017385">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038097">
        <property type="entry name" value="V-ATP_synth_e1/e2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026139018" description="V-type proton ATPase subunit" evidence="10">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001353056.3"/>
        </source>
    </evidence>
    <sequence length="85" mass="9480" checksum="EDA43036E341746E" modified="2020-08-12" version="1">MGAAFFPVLFFTALWGAVGIGMPIMTPKGPHQNLIRCILMLTAACCWLFWLCCYMAQMNPLIGPKLKRDVVAMIGRSWDMKIVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDK5</accession>
    <name>A0A6I8UDK5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pseudouridylate synthase 7 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Pus7</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
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    <dbReference type="RefSeq" id="XP_001353815.3">
        <property type="nucleotide sequence ID" value="XM_001353779.4"/>
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        <property type="interactions" value="1935"/>
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    <dbReference type="KEGG" id="dpo:4813576"/>
    <dbReference type="InParanoid" id="A0A6I8UDK5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="baseline"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
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        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
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    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
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        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF31">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
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    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="6">
        <location>
            <begin position="339"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="480"/>
            <end position="506"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="673"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="197"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="700"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="712"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001353815.3"/>
        </source>
    </evidence>
    <sequence length="721" mass="81845" checksum="E84F48D62A155AD7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFQ2</accession>
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        <recommendedName>
            <fullName evidence="3">NudC domain-containing protein 1</fullName>
        </recommendedName>
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        <name evidence="8" type="primary">LOC4815269</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
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    </reference>
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        <subcellularLocation>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="PANTHER" id="PTHR21664:SF1">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001354754.2"/>
        </source>
    </evidence>
    <sequence length="581" mass="65187" checksum="C664C84F0BB14460" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG87</accession>
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        <submittedName>
            <fullName evidence="6">Monocarboxylate transporter 7</fullName>
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        <name evidence="6" type="primary">LOC4814704</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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                <consortium name="RefSeq"/>
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    <dbReference type="PANTHER" id="PTHR11360:SF309">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 7-LIKE PROTEIN"/>
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    </dbReference>
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    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
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        <property type="entry name" value="MFS"/>
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            <end position="42"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="54"/>
            <end position="73"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="401"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="423"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="489"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="16"/>
            <end position="521"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="311"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001355008.3"/>
        </source>
    </evidence>
    <sequence length="542" mass="59997" checksum="346848B2ADE78C24" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHE1</accession>
    <name>A0A6I8UHE1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuole membrane protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Tango5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355546.3">
        <property type="nucleotide sequence ID" value="XM_001355510.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHE1"/>
    <dbReference type="FunCoup" id="A0A6I8UHE1">
        <property type="interactions" value="1164"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815718"/>
    <dbReference type="InParanoid" id="A0A6I8UHE1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHE1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="495"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355546.3"/>
        </source>
    </evidence>
    <sequence length="541" mass="59538" checksum="4EFE4E692E55F4F9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIB6</accession>
    <name>A0A6I8UIB6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">39S ribosomal protein L28, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mRpL28</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355947.3">
        <property type="nucleotide sequence ID" value="XM_001355911.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIB6"/>
    <dbReference type="FunCoup" id="A0A6I8UIB6">
        <property type="interactions" value="604"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816670"/>
    <dbReference type="InParanoid" id="A0A6I8UIB6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIB6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribosomal_bL28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L28, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL28M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355947.3"/>
        </source>
    </evidence>
    <sequence length="301" mass="34686" checksum="DE5696E60E92A035" modified="2020-08-12" version="1">MAHATPQGFKLLNGWKRPGRFDKGLGAQLPEAYKKFWREWKLTKPAAVHYIPKESEWERDEVTNVIKPVQNIPLPLIDTPESHQGIWGGEAVIKGFQKRQQTKRRVPHFWVPQLRRSVVHSQVLNEYISVIVTERALEQIHECHGFDHYLLKNLACDLRSALALKLKRQLLLSLQAGCPALADDPQHQQEVLKEYSRYLEPYTPEEIDWYGHTYLEAIRKLQGQLRDAEKVQPHKVEFRSKLIEQLREAGIREAGKLEKPDILAASSEHKDADIEALTNLESSPSSSNWLSKINPFGKKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK84</accession>
    <name>A0A6I8UK84_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein-glucosylgalactosylhydroxylysine glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4817014</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 65 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356841.3">
        <property type="nucleotide sequence ID" value="XM_001356805.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK84"/>
    <dbReference type="FunCoup" id="A0A6I8UK84">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817014"/>
    <dbReference type="InParanoid" id="A0A6I8UK84"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK84">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000032">
        <property type="entry name" value="Vacuolar acid trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005195">
        <property type="entry name" value="Glyco_hydro_65_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051:SF8">
        <property type="entry name" value="PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03632">
        <property type="entry name" value="Glyco_hydro_65m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026073467" evidence="4">
        <location>
            <begin position="24"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 central catalytic" evidence="5">
        <location>
            <begin position="337"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001356841.3"/>
        </source>
    </evidence>
    <sequence length="744" mass="84666" checksum="8F46D5F2F4FF4330" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKE2</accession>
    <name>A0A6I8UKE2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4817514</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356932.3">
        <property type="nucleotide sequence ID" value="XM_001356896.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKE2"/>
    <dbReference type="FunCoup" id="A0A6I8UKE2">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817514"/>
    <dbReference type="InParanoid" id="A0A6I8UKE2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356932.3"/>
        </source>
    </evidence>
    <sequence length="389" mass="45637" checksum="69C85F52AE58DD39" modified="2020-08-12" version="1">MPQQPHRGYRHRVAHFTLKSTLYASWALGLFPFTYDSRTRQLTRSRWLLSYGLVLNLGLIGVVLLPGTEDHRDVRIDMFERNPIIQQVENMVEIISFLTAVAMHLGIFWKSREMVTILNELFLLEKRHFSNLILAHCHQFDKYVIQKCILVVLEVGSSLLIYFGVPDSNLVVTRAFCIYLVQVGVLLGVTHFHLAVIYIYRFVWTINGQLLELANQQRRGQKVDPARIKLLFWLYSRLLEVNSRLAAIYDIPVTLFMVTLMSANIMIAHVLIIIWINQFSLLDILLLFPQALLINFYDLWLSIAFCELVESTGRQTSDILKLYNDGEDMDEELQRSLSDFALFCSHRRLRFRHCGLFYVNYEMGFRMIITNILYLVFLVQFDYMNLKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULQ6</accession>
    <name>A0A6I8ULQ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4800361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357553.2">
        <property type="nucleotide sequence ID" value="XM_001357516.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULQ6"/>
    <dbReference type="FunCoup" id="A0A6I8ULQ6">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="GeneID" id="4800361"/>
    <dbReference type="KEGG" id="dpo:4800361"/>
    <dbReference type="InParanoid" id="A0A6I8ULQ6"/>
    <dbReference type="OMA" id="HKSHAWV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081053">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31535">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31535:SF3">
        <property type="entry name" value="REGULATORY PROTEIN ZESTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026294981" evidence="2">
        <location>
            <begin position="23"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357553.2"/>
        </source>
    </evidence>
    <sequence length="538" mass="57890" checksum="ED440CAC50E293BC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULV5</accession>
    <name>A0A6I8ULV5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prolyl 4-hydroxylase subunit alpha-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4800288</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_001357607.3">
        <property type="nucleotide sequence ID" value="XM_001357570.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULV5"/>
    <dbReference type="FunCoup" id="A0A6I8ULV5">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800288"/>
    <dbReference type="InParanoid" id="A0A6I8ULV5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026081836" evidence="7">
        <location>
            <begin position="21"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Prolyl 4-hydroxylase alpha subunit" evidence="8">
        <location>
            <begin position="313"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357607.3"/>
        </source>
    </evidence>
    <sequence length="480" mass="55547" checksum="E9516E2E4F6E9CBB" modified="2020-08-12" version="1">MAALRYTLLLLCLACPFLEAVLDEKRFQGMLTLERELIQNLRDYALELTGKISLINGALREYNEEIEEANEDPEVYLSNPLNAFRLIRHMHQDWVSWQVYMEEQVGPDQVAKLERILPQLPQRQDFQAAAQTVQSLAEFYAYEPSFLVAQKEFYSNLYLSPLDCYHMGLEIYKKENYHEAIKWFTVAAENYTASPYSDLYSVLGTSRSEAYRMQARTYDKLNREEVFLAYQNAVILDEYNVHLLQESGVQELRSLREPEPMKPIEAPVTTPSPLQRQCQESFDAHFAVCAYNSILSPFLWLAPLRYEEVLSSPYIVIYRDAFYETEVAHIRDALKRCPLEAMIHMDGGIEGCSIPDEYSPVMKRLEQRVQDMTGYFKPADNFHVLEYAQQEPFRLFQLFKDSSKFSDLDFNEIEGTAIFFLNDVEVGGAITIPHYPMSVHPRQGDVLITFHEGDFEHTVCPNVVGSGLVLLKFLYVDPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMG5</accession>
    <name>A0A6I8UMG5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">betaTub85D</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357842.1">
        <property type="nucleotide sequence ID" value="XM_001357805.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMG5"/>
    <dbReference type="SMR" id="A0A6I8UMG5"/>
    <dbReference type="FunCoup" id="A0A6I8UMG5">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="GeneID" id="4800596"/>
    <dbReference type="KEGG" id="dpo:4800596"/>
    <dbReference type="InParanoid" id="A0A6I8UMG5"/>
    <dbReference type="OMA" id="WVPRSVN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081713">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000006">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="429"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001357842.1"/>
        </source>
    </evidence>
    <sequence length="446" mass="49870" checksum="E11495E67158E358" modified="2020-08-12" version="1">MREIVHIQAGQCGNQIGGKFWEVISDEHCIDATGTYYGDSDLQLERINVYYNEATGAKYVPRAILVDLEPGTMDSVRSGAFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSFFVEWIPNNCKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATADEEGEFDEDEEGGGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMX8</accession>
    <name>A0A6I8UMX8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrator complex subunit 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">IntS12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Integrator subunit 12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357964.2">
        <property type="nucleotide sequence ID" value="XM_001357927.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMX8"/>
    <dbReference type="FunCoup" id="A0A6I8UMX8">
        <property type="interactions" value="511"/>
    </dbReference>
    <dbReference type="GeneID" id="4800747"/>
    <dbReference type="KEGG" id="dpo:4800747"/>
    <dbReference type="InParanoid" id="A0A6I8UMX8"/>
    <dbReference type="OMA" id="KMISKNM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078922">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160232">
        <property type="term" value="C:INTAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160240">
        <property type="term" value="P:RNA polymerase II transcription initiation surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15501">
        <property type="entry name" value="PHD_Int12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000101">
        <property type="entry name" value="Integrator complex subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039054">
        <property type="entry name" value="Int12_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051776">
        <property type="entry name" value="Integrator_subunit_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13415:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13415">
        <property type="entry name" value="NUCLEAR FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="127"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="216"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001357964.2"/>
        </source>
    </evidence>
    <sequence length="340" mass="35511" checksum="F6F9FD6040B9EA26" modified="2020-08-12" version="1">MAANIAAAVAVPQEVDPVLKKAIKLLHSSNSTSASELRLLLDEALRARYGPEKSITQNMTQRMMDDEMSFPGRAPTPPQPINTDEIINLTNSPDKDPSDSVDTIIDSDECLSAAGIVNTGDTGDFGDLNCCVCGEMMFTATNRLVECSKCGAMYHQECHKPPITKEEAADDQEQTWQCDTCCNKPTSSRSVATAAAAATLPPTIFIADDPLPLAASKTSKTASGASSRSSTSSNSSSPFYRPEPSSSTNASSSSSSKQGHKSSSSSSSKSHKEERASKSVAPSLTAISGVEKHSAGPSSSTSRRSSSNTKSSSSKSSSSKHHESSSSSGGGGGSKRRSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNX9</accession>
    <name>A0A6I8UNX9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc finger protein 800</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4801485</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358568.4">
        <property type="nucleotide sequence ID" value="XM_001358531.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNX9"/>
    <dbReference type="FunCoup" id="A0A6I8UNX9">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801485"/>
    <dbReference type="InParanoid" id="A0A6I8UNX9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039149">
        <property type="entry name" value="ZNF800"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21020">
        <property type="entry name" value="ZINC FINGER PROTEIN 800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21020:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="277"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="524"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="513"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="634"/>
            <end position="701"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="847"/>
            <end position="943"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="679"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="872"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="894"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="915"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358568.4"/>
        </source>
    </evidence>
    <sequence length="967" mass="107309" checksum="E24FB4CBCD532918" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP51</accession>
    <name>A0A6I8UP51_DROPS</name>
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        <submittedName>
            <fullName evidence="19">Protein kinase C and casein kinase II substrate protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="19" type="primary">Synd</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. May form heterooligomers with other PACSINs. Interacts (via SH3 domain) with DNM1, SYNJ1 and WASL. Interacts with TRPV4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358625.3">
        <property type="nucleotide sequence ID" value="XM_001358588.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP51"/>
    <dbReference type="FunCoup" id="A0A6I8UP51">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="GeneID" id="4801553"/>
    <dbReference type="KEGG" id="dpo:4801553"/>
    <dbReference type="InParanoid" id="A0A6I8UP51"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077289">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07655">
        <property type="entry name" value="F-BAR_PACSIN"/>
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    <dbReference type="CDD" id="cd11843">
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    <dbReference type="FunFam" id="2.30.30.40:FF:000014">
        <property type="entry name" value="Kinase C and casein kinase substrate in neurons protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000009">
        <property type="entry name" value="Protein kinase C and casein kinase substrate in neurons 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
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        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF11">
        <property type="entry name" value="SYNDAPIN, ISOFORM C"/>
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        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026162739" evidence="15">
        <location>
            <begin position="36"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="F-BAR" evidence="17">
        <location>
            <begin position="41"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="476"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="372"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="376"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001358625.3"/>
        </source>
    </evidence>
    <sequence length="536" mass="60431" checksum="A42A56659532A22A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPG3</accession>
    <name>A0A6I8UPG3_DROPS</name>
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        <submittedName>
            <fullName evidence="16">Syntaxin-1A</fullName>
        </submittedName>
    </protein>
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        <name evidence="16" type="primary">Syx1A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <text evidence="1 10">Belongs to the syntaxin family.</text>
    </comment>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="Bgee" id="FBgn0076052">
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    </dbReference>
    <dbReference type="GO" id="GO:0012505">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15880">
        <property type="entry name" value="SNARE_syntaxin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000042">
        <property type="entry name" value="Syntaxin 11b, tandem duplicate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000005">
        <property type="entry name" value="Syntaxin 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF424">
        <property type="entry name" value="SYNTAXIN-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="195"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="40"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001358807.3"/>
        </source>
    </evidence>
    <sequence length="296" mass="34174" checksum="273B08DD672B2564" modified="2020-08-12" version="1">MTKDRLAALHAAQSDDEESEEVAVNVDGHDSYMDDFFSQVEEIRGMIDKVQDNVEEVKKKHSAILSAPQTDEKTKQELEDLMADIKKNANRVRGKLKGIEQNIEQEEQQNKSTADLRIRKTQHSTLSRKFVEVMTEYNRTQTDYRERCKGRIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGIIMETQQAKQTLADIEARHQDIMKLETSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKALKYQSKARRKKIMILICLTVLGILAASYVSSYFIIQASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQL6</accession>
    <name>A0A6I8UQL6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Pickpocket protein 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">rpk</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359376.4">
        <property type="nucleotide sequence ID" value="XM_001359339.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQL6"/>
    <dbReference type="FunCoup" id="A0A6I8UQL6">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802459"/>
    <dbReference type="InParanoid" id="A0A6I8UQL6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000008">
        <property type="entry name" value="pickpocket protein 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001359376.4"/>
        </source>
    </evidence>
    <sequence length="579" mass="66193" checksum="CCD762865CD28870" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQR1</accession>
    <name>A0A6I8UQR1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein FAM114A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4802542</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM114 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359441.2">
        <property type="nucleotide sequence ID" value="XM_001359404.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQR1"/>
    <dbReference type="FunCoup" id="A0A6I8UQR1">
        <property type="interactions" value="520"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802542"/>
    <dbReference type="InParanoid" id="A0A6I8UQR1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007998">
        <property type="entry name" value="DUF719"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12842">
        <property type="entry name" value="FI01459P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12842:SF6">
        <property type="entry name" value="FI01459P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05334">
        <property type="entry name" value="DUF719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359441.2"/>
        </source>
    </evidence>
    <sequence length="470" mass="51977" checksum="38BDBD98DBD5A65F" modified="2020-08-12" version="1">MAKKLEKHEEASNDWNQSDEDDNWDDWGDDDDKFVHVEKEQQQESSDDRNNAKGKEEKPLEQLTSQAETTAPAPELSSSSSWGLWGGKLSSVLNTATEGLGNITTQVNQGLSQIIGVPDPEELARLNAAEKAKEETTVVSPKETPSATPVDPNQENAFGLGFVTNLGSKVLNTGLDTLEGIGKKTMTILQDNDPKLMNKRKLLGLEANKPNLSALLLEAKKDADQMEESLMQLQLEKKKAQLRFEILFENYCGLVHYEALEILSKESRLKLDTLLDAVSGNALADLQETINEVKELLELEDLECDSEGDYKPEELGERLSATIADAELGIQFEEVANHWKQSLTWLKSEEATTSDLEQAYAKSIHALSEACALEMCKLHKIAELLLVKQHHSTANEVDGIVNLCKQCNGHLQGLSHRYAALLSSKGASSPDTAEESKERVNTFFAEMFAAVQYVEQAYKLFTPILQMGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQX5</accession>
    <name>A0A6I8UQX5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Beta-catenin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4802622</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359507.1">
        <property type="nucleotide sequence ID" value="XM_001359470.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQX5"/>
    <dbReference type="FunCoup" id="A0A6I8UQX5">
        <property type="interactions" value="2785"/>
    </dbReference>
    <dbReference type="GeneID" id="4802622"/>
    <dbReference type="KEGG" id="dpo:4802622"/>
    <dbReference type="InParanoid" id="A0A6I8UQX5"/>
    <dbReference type="OMA" id="TDWREQE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071353">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000376">
        <property type="entry name" value="beta-catenin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039678">
        <property type="entry name" value="CTNNBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013180">
        <property type="entry name" value="CTNNBL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978:SF0">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1 NUCLEAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08216">
        <property type="entry name" value="CTNNBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01156">
        <property type="entry name" value="DUF1716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta-catenin-like protein 1 N-terminal" evidence="7">
        <location>
            <begin position="80"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359507.1"/>
        </source>
    </evidence>
    <sequence length="581" mass="66152" checksum="1C292697744C5AE8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URF2</accession>
    <name>A0A6I8URF2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">alphaTub84B</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359737.1">
        <property type="nucleotide sequence ID" value="XM_001359700.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URF2"/>
    <dbReference type="SMR" id="A0A6I8URF2"/>
    <dbReference type="FunCoup" id="A0A6I8URF2">
        <property type="interactions" value="774"/>
    </dbReference>
    <dbReference type="GeneID" id="4802911"/>
    <dbReference type="KEGG" id="dpo:4802911"/>
    <dbReference type="InParanoid" id="A0A6I8URF2"/>
    <dbReference type="OMA" id="YMASCIL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075153">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="248"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001359737.1"/>
        </source>
    </evidence>
    <sequence length="450" mass="49908" checksum="E4521F429F5045D2" modified="2020-08-12" version="1">MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTVGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDSGDGEGEGAEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URI0</accession>
    <name>A0A6I8URI0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">alpha-Est7</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001359771.3">
        <property type="nucleotide sequence ID" value="XM_001359734.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URI0"/>
    <dbReference type="FunCoup" id="A0A6I8URI0">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="ESTHER" id="drops-q294p0">
        <property type="family name" value="Carb_B_Arthropoda"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802957"/>
    <dbReference type="InParanoid" id="A0A6I8URI0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000092">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="34"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359771.3"/>
        </source>
    </evidence>
    <sequence length="572" mass="65223" checksum="53638ACD1985D402" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URI4</accession>
    <name>A0A6I8URI4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Growth hormone-inducible transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Mics1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359772.1">
        <property type="nucleotide sequence ID" value="XM_001359735.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URI4"/>
    <dbReference type="FunCoup" id="A0A6I8URI4">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="GeneID" id="4802958"/>
    <dbReference type="KEGG" id="dpo:4802958"/>
    <dbReference type="InParanoid" id="A0A6I8URI4"/>
    <dbReference type="OMA" id="TLMWSER"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071920">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10431">
        <property type="entry name" value="GHITM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035871">
        <property type="entry name" value="GHITM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF112">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359772.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="39280" checksum="E57C4D9B7A535198" modified="2020-08-12" version="1">MLLARLNHVLPGRSLCLLGQVTSQRLWQSTKAMPLLKKNPLTWPSMGQGKSLIPKKDLQNMLRRDYSRESNRNRSHLESRTRGPSLKDRMMGPPSENAYSMGKGAAAGAALMGLVGLCYYGLGLSKQTSVYDQSLLWPQYVRDRIHATYAYFGASCGITAAAAAAFFQSETAMSLMTRSGIVAGLITLGLVMGTGVMAQSIEYKEGFGAKQLAWLAHCTVLGAVLAPMCFLGGPILTRAMLYTGGIVGALSTVAACAPSDKFLYMGGPLAIGLGVVFASSLAGMWLPPTTALGAGLASMSVYGGLILFSGFLLYDTQRIVRVAELHPQHGIPKFDPINMALAIYMDALNIFIRIAMILASDNQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USE8</accession>
    <name>A0A6I8USE8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UDP-glucuronosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Ugt304A1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359924.3">
        <property type="nucleotide sequence ID" value="XM_001359887.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USE8"/>
    <dbReference type="FunCoup" id="A0A6I8USE8">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803131"/>
    <dbReference type="InParanoid" id="A0A6I8USE8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026347960" evidence="5">
        <location>
            <begin position="22"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359924.3"/>
        </source>
    </evidence>
    <sequence length="522" mass="60020" checksum="129DC824B69A5348" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USG2</accession>
    <name>A0A6I8USG2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="16">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Pgant3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Golgi apparatus membrane</location>
            <topology evidence="2 16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="16"/>
    <dbReference type="RefSeq" id="XP_001360187.1">
        <property type="nucleotide sequence ID" value="XM_001360150.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USG2"/>
    <dbReference type="FunCoup" id="A0A6I8USG2">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="GeneID" id="4803469"/>
    <dbReference type="KEGG" id="dpo:4803469"/>
    <dbReference type="InParanoid" id="A0A6I8USG2"/>
    <dbReference type="OMA" id="LWTYDMD"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078194">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF118">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 16" id="KW-0430">Lectin</keyword>
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    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
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    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="17">
        <location>
            <begin position="513"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001360187.1"/>
        </source>
    </evidence>
    <sequence length="667" mass="77003" checksum="A31BD8813B605E3A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USK3</accession>
    <name>A0A6I8USK3_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Wnt inhibitory factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4803557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360264.1">
        <property type="nucleotide sequence ID" value="XM_001360227.4"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026060579" evidence="1">
        <location>
            <begin position="20"/>
            <end position="206"/>
        </location>
    </feature>
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        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="88"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="133"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360264.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="23310" checksum="8CA021AAD7FD1FFA" modified="2020-08-12" version="1">MLGRTVFLQLALQHVLVQGYYYYDYGYWEINEPPPLCSDYEMLIVNTRQCVRRCNIVCLGGVCFEDGDCPCADRYLGANPDGLVCAAECLPGCREAGGYCAAPDLCVCRKDRHYYFDALSRRCRHRGARLLDPCHGRCTHGRCSYDGSCICAQGYELRTTLLHGQQCTPICQHDCGPHAYCFSPNMCACRHKHHHYAHNGICTKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USU3</accession>
    <name>A0A6I8USU3_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Immune-induced peptides</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BaraC</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360368.3">
        <property type="nucleotide sequence ID" value="XM_001360331.4"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026245919" evidence="2">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360368.3"/>
        </source>
    </evidence>
    <sequence length="112" mass="11846" checksum="CE7EE96D829ECE3C" modified="2020-08-12" version="1">MRYFAVICIVVASMLFAAVSAQTSSDGNGPHQFGSPENGIYIRGQNEGPYNVPGVTGTFQNSPSSGRHAYTDENGNTYTHFHSQSGGSAAKGLTLAIPSVLVFISVVLMRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU72</accession>
    <name>A0A6I8UU72_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyltransferase C9orf114 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4804080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360698.2">
        <property type="nucleotide sequence ID" value="XM_001360661.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU72"/>
    <dbReference type="FunCoup" id="A0A6I8UU72">
        <property type="interactions" value="909"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804080"/>
    <dbReference type="InParanoid" id="A0A6I8UU72"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="476"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360698.2"/>
        </source>
    </evidence>
    <sequence length="501" mass="55282" checksum="3E10115FF15FA2DA" modified="2020-08-12" version="1">MKAMLAAPAAVRNGKPTEPHKSWKKVNEERKALKRERKQEKLLKQMNQTKESEANADAAASAAQARMNKPNPSTLSIAVPGSILENAQSAELRAYVAGQIARAACIFRVNEVIVFDDVGVATARETKRSYEQDAEGNGTGTVRSSSLQLARILQYLECPQYLRKYFFPLHKDLKYSGLLNPLDTPHHLRQQSKFRYREGVISDKKAKDGQSYANVGLLNDVLVDKAIEPGVRVTVKMDPASDTSRKQRGTLVSPDEPRRETGVYWGYQVRIAHSLSEIFTKSPYESSYDVTLGTSDRGTNVHEVPNRSIQYKHLLIVFGGLQGLEEALANDDKLTVDDPEMLFDHYVNVLPRQGSRTIRTEEALLIALASLQEKLQPELADVETDLSDLLPRSEDTGIPMGRDALISKKQKKRKLVEAPHETVADEPAPKEAAPSPKKIFPAPKASLPTPARLSANPFGDPSATKTSLEDDFEVVPTTTAGPASNRKTNASDSGDDLSRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU99</accession>
    <name>A0A6I8UU99_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PAS domain-containing serine/threonine-protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Pask</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360871.4">
        <property type="nucleotide sequence ID" value="XM_001360834.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU99"/>
    <dbReference type="FunCoup" id="A0A6I8UU99">
        <property type="interactions" value="1858"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804291"/>
    <dbReference type="InParanoid" id="A0A6I8UU99"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14004">
        <property type="entry name" value="STKc_PASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000351">
        <property type="entry name" value="PAS domain-containing serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="51"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="554"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="587"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360871.4"/>
        </source>
    </evidence>
    <sequence length="965" mass="108815" checksum="0384726E0637357B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW26</accession>
    <name>A0A6I8UW26_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4805290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361712.4">
        <property type="nucleotide sequence ID" value="XM_001361675.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW26"/>
    <dbReference type="GeneID" id="4805290"/>
    <dbReference type="KEGG" id="dpo:4805290"/>
    <dbReference type="InParanoid" id="A0A6I8UW26"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073918">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361712.4"/>
        </source>
    </evidence>
    <sequence length="207" mass="22640" checksum="D7828CB5AB070C0D" modified="2020-08-12" version="1">MEYHRPADAQTMLLIMIVIIGCSTTAHGQSYLIALENVLNESPQPQHQPPYNLTTANSTTDVDPFANSTRIIVYKNTVVEPSNELSQTAMDVITIVWYVATFLALAAFFMLMACSDRRCRDMRRHHSGAGAQSEGVRAPPTPSPSYSEFAPPSYDTVIKMQHAAKTSVFVIPFNKASDPGGNPAPTSQPPPVTCNIYTVHELQKSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWJ8</accession>
    <name>A0A6I8UWJ8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein NDNF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12 13 14" type="primary">nord</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="11 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 14">MV-25-SWS-2005</strain>
            <tissue evidence="11 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361996.2">
        <property type="nucleotide sequence ID" value="XM_001361959.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040741.1">
        <property type="nucleotide sequence ID" value="XM_015185255.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040742.1">
        <property type="nucleotide sequence ID" value="XM_015185256.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233719.1">
        <property type="nucleotide sequence ID" value="XM_033377828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWJ8"/>
    <dbReference type="GeneID" id="4805624"/>
    <dbReference type="KEGG" id="dpo:4805624"/>
    <dbReference type="OMA" id="PCAGSEI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075848">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019326">
        <property type="entry name" value="NDNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045805">
        <property type="entry name" value="NDNF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055271">
        <property type="entry name" value="NDNF_Fn(III)_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14619">
        <property type="entry name" value="NEURON-DERIVED NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14619:SF3">
        <property type="entry name" value="PROTEIN NDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10179">
        <property type="entry name" value="NDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19433">
        <property type="entry name" value="NDNF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634458" description="Protein NDNF" evidence="7">
        <location>
            <begin position="19"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Neuron-derived neurotrophic factor first Fn(III)" evidence="8">
        <location>
            <begin position="198"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Protein NDNF C-terminal" evidence="9">
        <location>
            <begin position="410"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF19433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001361996.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015040741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015040742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033233719.1"/>
        </source>
    </evidence>
    <sequence length="577" mass="65586" checksum="30866C4DC51B6C01" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWX4</accession>
    <name>A0A6I8UWX4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6903423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132302.2">
        <property type="nucleotide sequence ID" value="XM_002132266.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWX4"/>
    <dbReference type="KEGG" id="dpo:6903423"/>
    <dbReference type="InParanoid" id="A0A6I8UWX4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026229657" evidence="2">
        <location>
            <begin position="17"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002132302.2"/>
        </source>
    </evidence>
    <sequence length="194" mass="23451" checksum="3274B8694FF4D819" modified="2020-08-12" version="1">MWIFPILLLLVQHSTGSLLPRESPHEDTTISEERQSQCRKEFEEQNQQLTSLKAQLNVYRDKARELEMLLNYSNTMGERLWESYKAQTDFKEQVEKILMKEIQVQNSTIQLEEDQRQSFEDRINELEWQLKNRDLKIKEIETQMKHKEDRTRGYLKTFMDIIEKELEGKSKEVLIRRLSRMLIFMQIKDPHLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX95</accession>
    <name>A0A6I8UX95_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC6902785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132522.3">
        <property type="nucleotide sequence ID" value="XM_002132486.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015180263.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX95"/>
    <dbReference type="KEGG" id="dpo:6902785"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UX95">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132522.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015035749.2"/>
        </source>
    </evidence>
    <sequence length="120" mass="14008" checksum="4063EEB85EE4499E" modified="2020-08-12" version="1">MLNRQPDYNISDLGYPWPCDTHMKMRRKLPKACGMPPASPNRLLPMRETLNDYFQRLIRQEEDQDDLQARQKHHKNQWNEQQRRRNQKCRDKGETATATGTATATTATTTTIATCDLNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXB0</accession>
    <name>A0A6I8UXB0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein regulatory complex protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132589.3">
        <property type="nucleotide sequence ID" value="XM_002132553.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXB0"/>
    <dbReference type="KEGG" id="dpo:6902853"/>
    <dbReference type="InParanoid" id="A0A6I8UXB0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXB0">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="363"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132589.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="54892" checksum="4F78A32CAE7CA7FE" modified="2020-08-12" version="1">MKVNTIGEQDTQMQDERTLDLDDEEQAAERCAGGVEYPLDGGVEMESVYNIKFPLPEQTDPELCRDEFLKNVLEQYKTKVEKKTRSQRSRELTFIGLDGPEKTLEALAIKEMIRRSRMSSRGTTVVSIIQRRTTRLYEITLECQKASVDFMLPRLRKQLGLFSNPWPGEINNMPNKLFHWSVDHFMQRLHINQLYLDVIQRERTKSDLDCVKFRTDLHEIISLLQEVRDDFIEGRDICEYSMKLIHAAKYTTNPKWVHNFNMARQLQEELKYNSFTNRGSKNYRRSTDNRQSGMKIDFEHQQAVGPIDLRYRINWMHSCSDQVLMRLQAREELLQTELTEIRTHMKQDSMVHNNSEFVYAYLIDSFKNKIKDWEEKLETDMEKAELMCNQTSNKLQKVKDDLVFYRDQQEMFKRRIIEVRAQIEQEERDRIEEAEMRATALSLKLLNKKNKRRGKDKKKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXF5</accession>
    <name>A0A6I8UXF5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial import receptor subunit TOM20 homolog B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6902917</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132647.2">
        <property type="nucleotide sequence ID" value="XM_002132611.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXF5"/>
    <dbReference type="KEGG" id="dpo:6902917"/>
    <dbReference type="InParanoid" id="A0A6I8UXF5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXF5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002132647.2"/>
        </source>
    </evidence>
    <sequence length="201" mass="23370" checksum="23B5CDF4971A49A7" modified="2020-08-12" version="1">MLSVNLFLLITSGVSFVGYCLFFDRKRLADPEYKRKLHERRLQKATREDQALEEEEEEEPEFGDDEQAVVSQQQYQHRLQLETFIWMSAVRCLEHPTILKRMFESEVQLGDRLLQDGRMDEALAHLANAILLCKQPVVLLQSLQKTMPKELIHPLIIRVSELLEESHHQENDREDQEAAFSSSDSDEWISDGNVPQPHMVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZD0</accession>
    <name>A0A6I8UZD0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein C7orf26 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6901289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133990.2">
        <property type="nucleotide sequence ID" value="XM_002133954.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZD0"/>
    <dbReference type="KEGG" id="dpo:6901289"/>
    <dbReference type="InParanoid" id="A0A6I8UZD0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZD0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027844">
        <property type="entry name" value="INTS15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14540">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14540:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14964">
        <property type="entry name" value="INTS15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002133990.2"/>
        </source>
    </evidence>
    <sequence length="367" mass="40142" checksum="09591C5983F1DA4F" modified="2020-08-12" version="1">MSSSVAARGKASRLNPSLKPENFPKCASEALILLETLLVGRNTQDLVIKIVSENIFLERINDGGGRATQMAFMQEYQLTIALIEFFSKPGPRRDDIFHALFGSKLSPRRSSQLVKLVSTAVSASVVPVLTAAGAWLQQVGYKSTLSQEVTKQIAADFTLHSLPARQKLEQLSMIVPHFAASFMVAVADLYLNEKRSAGLTPPPEALLDVFTEWMTENPTVICQAPPPGLGLPAGATPGPFVNPLTSLLRWTVLAPLVSQGATYSKLHLSLLTAIQQVAAVNKSTVLPNRELMQIIKSLQSYCARLRARKVKPEHDDAYQRSMARFAQAVQIALSSKCIANRYPLLAAVKLLPPFKLMSIVISSQKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZL2</accession>
    <name>A0A6I8UZL2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6901528</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134202.2">
        <property type="nucleotide sequence ID" value="XM_002134166.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZL2"/>
    <dbReference type="KEGG" id="dpo:6901528"/>
    <dbReference type="InParanoid" id="A0A6I8UZL2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026247387" evidence="3">
        <location>
            <begin position="19"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002134202.2"/>
        </source>
    </evidence>
    <sequence length="220" mass="25424" checksum="4C7244EC510E78A3" modified="2020-08-12" version="1">MWWIFAIFLLFLLQHSRASIVRVQPVVSEDSVKVSVQLLTQCSSYCLLIAEPVLQYAEKCQENSRAYQEKNDQLNRLQAHLNVYMDKAREMEKLLNYSNTIREVLGESHTNQTNFSSDVENIISALEIGNQRRIIKDLEKQLENRNVQIREAEYAIEKIKAECLKESITSQDVRTKQQDKTINNMKLEVEQQVTQSEASIEDIKEQSKTRSLINGSANEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0M7</accession>
    <name>A0A6I8V0M7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Gr64d</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in the sugar gustatory response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr5a subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134986.2">
        <property type="nucleotide sequence ID" value="XM_002134950.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0M7"/>
    <dbReference type="KEGG" id="dpo:4813522"/>
    <dbReference type="InParanoid" id="A0A6I8V0M7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0M7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033041">
        <property type="term" value="F:sweet taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009318">
        <property type="entry name" value="Gustatory_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21421">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21421:SF35">
        <property type="entry name" value="GUSTATORY RECEPTOR FOR SUGAR TASTE 64B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06151">
        <property type="entry name" value="Trehalose_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038981">
        <property type="entry name" value="GRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002134986.2"/>
        </source>
    </evidence>
    <sequence length="421" mass="49086" checksum="B5181E34F24FA769" modified="2020-08-12" version="1">MEWSAQSVPNKNTLHHAIGYVLVVAQFFGVLPLSGVEPSVPVASVRFRWFSPLNLLPVAALCFVLLDFVLSAKLVIQNGLKLYTIGSLSFSVICIFCFGAFLLLAPRWPHIIRQTFECERIFLQSCYNSSIGRRFSQRLRCWAIALLVTALCEHLSYVVSAVWSNWKQIRECHLDIDFWQNYFLRERQELFSILPYSTWFALYVEWCTLSMTFVWNFVDIFLILVCRSMQMRFQQLHWRIRQHIGRRMSDEFWQEVRYDLLDLNDLLQLYDKELSGLVLVACANNMYFICVQIYHSFQVKGAVLDEVYFWFCLLYVVSRIVNMVLAASSIPQEAKQINFTLDEVPTSCWSKELERLNEIFHNEAFALSGKGYFVLTRRLLFTMAATLMVYELVLINQMEGEEVQRSICNRGAGSSMSIFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0P3</accession>
    <name>A0A6I8V0P3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial dicarboxylate carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Dic4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135172.2">
        <property type="nucleotide sequence ID" value="XM_002135136.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0P3"/>
    <dbReference type="FunCoup" id="A0A6I8V0P3">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900813"/>
    <dbReference type="InParanoid" id="A0A6I8V0P3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0P3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="13"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="105"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="201"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002135172.2"/>
        </source>
    </evidence>
    <sequence length="295" mass="32732" checksum="FE4CC3CA7B380D62" modified="2020-08-12" version="1">MHKIVYKKNKEKPELRARCWSAGVASTIVACVVAPVDLVKTHMQTQKNKKGMLQTAGKVMRLRGPLGFYNGFSAAAVRQMTSTNIRFGIYEVGRHADVVPNSNLAKLGLSGFAGAAASICGIPMDLINVRMQNDMKLPNGERRNYRNIVDAFVRISREEGWRALYTGGTSAVLKAAVNTCGQIGLYDIVKTKLSTRFGMKDDIVLHFDSSLISSIFSTVISHPFDVLKTLMMNARPGQFPSISHALKHMMRFGKLGPFRGLAPTMLRKGPATVCLLVFYEQLRLRFGYVDTKKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V148</accession>
    <name>A0A6I8V148_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135366.3">
        <property type="nucleotide sequence ID" value="XM_002135330.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V148"/>
    <dbReference type="KEGG" id="dpo:6900000"/>
    <dbReference type="InParanoid" id="A0A6I8V148"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249863">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V148">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026203049" description="MD-2-related lipid-recognition domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135366.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="21115" checksum="BB0CD70E22492C75" modified="2020-08-12" version="1">MWRLPVFVLWIALQSTSFSHVTFTNLKCSSLDDKFMEFATCQIKAVNRSHKYLDIYTKMHQLPINTAWVKVKFMKFDNGYKPFFIDLSYDACKFMRNKKKHPIAFMFFRSFVNNTNLNHTCPYDHDIFVDKLYTGELETEFSRLIPIPSGDYAIFTEWSSENTTRCTVRLYMKIFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1M1</accession>
    <name>A0A6I8V1M1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cuticle protein 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6900303</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135625.2">
        <property type="nucleotide sequence ID" value="XM_002135589.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1M1"/>
    <dbReference type="FunCoup" id="A0A6I8V1M1">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900303"/>
    <dbReference type="InParanoid" id="A0A6I8V1M1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF29">
        <property type="entry name" value="CUTICULAR PROTEIN 64AC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026259832" description="Cuticle protein 21" evidence="4">
        <location>
            <begin position="19"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002135625.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="38581" checksum="1522A3D894E157A5" modified="2020-08-12" version="1">MFAQTLLVLGLALSVAVAIPIDPYGSSISYAAPAVSYAAPAVSYAAPAISYAAPKLLAAPAISYAAPAISYAAPKLLAAPVAVAKVAVAEPYDPNPQYSFSYGVTDHKTGDSKQQEETLVNGVVHGSYSLAEPDGTIRKVTYTADKVNGFNAVVEKKGVAIAKPVLAVAAVPAVTKIGYASGPGLSLGGHYTWPLRQWPIRWVQFLATVATRRMSSKRATGGPLKPRSMGTQPQSRQRCQASTTPIQSSNPAASIAMALFKITIICCALFAAIECGLLPVGAPINTEVDPHPQYAFAYNVQDAITGDSKSQQEVRDGDVVKGSYSVVDADGSLRTVFYTADPINGFNAVVQRGPVPRPVLPAPLAAPLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1T0</accession>
    <name>A0A6I8V1T0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6899232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002136291.2">
        <property type="nucleotide sequence ID" value="XM_002136255.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1T0"/>
    <dbReference type="KEGG" id="dpo:6899232"/>
    <dbReference type="InParanoid" id="A0A6I8V1T0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002136291.2"/>
        </source>
    </evidence>
    <sequence length="315" mass="34193" checksum="80E0BEF4A55136C8" modified="2020-08-12" version="1">MDPQRATTKKVVREASSETEGSPQAETSCPVVQLEDAAAKNEVPPAAVDIPVVQLKDATVGSTETDVSPDVKKEPEASCPLDEMPSTSQQGTKEGQKPESGRSQGRLNHLIDMLDNFTIRLDVIEEKVRREDEECLDPEPVTLTVDSGTAVIIQIPEICELGTQTVVTRPPRRLAIPTAPPRNQPDTRAPCERTHHTIQIRSNNNRGNAAAAAAEQGPPSLGARIVRALGDFGAAFCLCLQINKDCIFCLGFFVAFVVSASFLTAFFYRTLNLTSPAARVPGDAFGGATGSYDVATLRFNGGYYYIYNRQRQQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1T7</accession>
    <name>A0A6I8V1T7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6896780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002136986.3">
        <property type="nucleotide sequence ID" value="XM_002136950.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1T7"/>
    <dbReference type="FunCoup" id="A0A6I8V1T7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896780"/>
    <dbReference type="InParanoid" id="A0A6I8V1T7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026333738" evidence="1">
        <location>
            <begin position="20"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002136986.3"/>
        </source>
    </evidence>
    <sequence length="87" mass="9753" checksum="B572042435002238" modified="2020-08-12" version="1">MKLTLLLILCVCLFGFVFGNTLVTKPPFLRSRYSLRWRKTTPAATEATTGHPMDLATSTEHPKMRTSTANADYDYYGNGEADNVVHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2G6</accession>
    <name>A0A6I8V2G6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6897052</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137236.2">
        <property type="nucleotide sequence ID" value="XM_002137200.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2G6"/>
    <dbReference type="KEGG" id="dpo:6897052"/>
    <dbReference type="InParanoid" id="A0A6I8V2G6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000007">
        <property type="entry name" value="Mitochondrial tricarboxylate transport protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF4">
        <property type="entry name" value="TRICARBOXYLATE TRANSPORT PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="17"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="117"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="212"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137236.2"/>
        </source>
    </evidence>
    <sequence length="303" mass="33023" checksum="3C5C63D6494F10C9" modified="2020-08-12" version="1">MQSPVPVPVLVAASSGEKGLKGIVAGGITGGLEILITYPTEFVKTQLQLDEKGEMKKFNGIADCVKKTVKQNGFSGLYRGLSVLLLGSIPKSAARFGAFEFFSNKMRDESGELPMTRRFFCGMLAGMTEAVVAVTPMETIKVRFINDQRSAKPKFKGLFHGVGQIVKSDGIGGIYKGLPATVMKQGSNQAIRFFVLFSLKDLYTGRDKTKTVPKPLVGVFGAIAGAASVFGNNPLDVVKTRMQSLDSARYKNTLDCAIQVLRQEGPMAFYKGTVPRLGRVCLDVAITFMIYDTIMDVFNLFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3E5</accession>
    <name>A0A6I8V3E5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transmembrane emp24 domain-containing protein eca-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6897811</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137916.4">
        <property type="nucleotide sequence ID" value="XM_002137880.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3E5"/>
    <dbReference type="KEGG" id="dpo:6897811"/>
    <dbReference type="InParanoid" id="A0A6I8V3E5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026196839" evidence="8">
        <location>
            <begin position="21"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="30"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002137916.4"/>
        </source>
    </evidence>
    <sequence length="358" mass="41275" checksum="9FC718BA454AF510" modified="2020-08-12" version="1">MAHRSACLILVLCTLHSACGLYFHMSETERKCFIEEVPDETDVKVYYKMELYDPRSNGFMPSSPGIGMHVEVRDSNNKIILSRVYRSQGWLFFTAHAPGEHVICMYSNSTAWFSGAQLRVHLDIQVGEHAINYANVAQKEKLTTDQLRIRQLLDQVEQITREQNYQRYREDRFRHTSESTNSRVLWWSLAQTVVLVCISTLQAFNLRNERLDLYRAFCKVLSGDEFTMFTSLDAFKALMEIIGTSPEAVTSSAISQWVDQVLDLDSSLTKPDEIMLVNLTQRLHDKVNEFAGKFLNNEKVSKENRDVIKPSYFLENSNSDLNIFLEQTKPDRSAHLSNDKFLLICECTECRPQSTIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3G4</accession>
    <name>A0A6I8V3G4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Yemanuclein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">yem</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137267.2">
        <property type="nucleotide sequence ID" value="XM_002137231.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3G4"/>
    <dbReference type="FunCoup" id="A0A6I8V3G4">
        <property type="interactions" value="1368"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897083"/>
    <dbReference type="InParanoid" id="A0A6I8V3G4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014840">
        <property type="entry name" value="HRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026947">
        <property type="entry name" value="UBN_middle_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21669">
        <property type="entry name" value="CAPZ-INTERACTING PROTEIN AND RELATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21669:SF28">
        <property type="entry name" value="YEMANUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08729">
        <property type="entry name" value="HUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14075">
        <property type="entry name" value="UBN_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hpc2-related" evidence="3">
        <location>
            <begin position="122"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Ubinuclein middle" evidence="4">
        <location>
            <begin position="442"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="643"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="891"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="966"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1080"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="231"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="295"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="658"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="696"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="730"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="742"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="966"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="984"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1086"/>
            <end position="1109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002137267.2"/>
        </source>
    </evidence>
    <sequence length="1109" mass="119191" checksum="F3E4FC91F4D7C5D0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3I5</accession>
    <name>A0A6I8V3I5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">28S ribosomal protein S21, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mRpS21</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137310.1">
        <property type="nucleotide sequence ID" value="XM_002137274.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3I5"/>
    <dbReference type="FunCoup" id="A0A6I8V3I5">
        <property type="interactions" value="642"/>
    </dbReference>
    <dbReference type="GeneID" id="6897129"/>
    <dbReference type="KEGG" id="dpo:6897129"/>
    <dbReference type="InParanoid" id="A0A6I8V3I5"/>
    <dbReference type="OMA" id="MRHAQFL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248502">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_bS21"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00030">
        <property type="entry name" value="S21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21109">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21109:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002137310.1"/>
        </source>
    </evidence>
    <sequence length="87" mass="10665" checksum="E2F276A7EB1C7206" modified="2020-08-12" version="1">MRHVQFLARTVLVQNNNVEEACRMLNRVLGKEEILDQFRRTRFYEKPYQVRRRVNFEKCKAIYNEDMNRKIQFVLRKNRVEPFPGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3U5</accession>
    <name>A0A6I8V3U5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serine protease inhibitor 42Dd</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Spn42Dd</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138171.2">
        <property type="nucleotide sequence ID" value="XM_002138135.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3U5"/>
    <dbReference type="FunCoup" id="A0A6I8V3U5">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898086"/>
    <dbReference type="InParanoid" id="A0A6I8V3U5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19954">
        <property type="entry name" value="serpin42Dd-like_insects"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF372">
        <property type="entry name" value="ACCESSORY GLAND PROTEIN ACP76A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026077423" evidence="4">
        <location>
            <begin position="19"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="5">
        <location>
            <begin position="20"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002138171.2"/>
        </source>
    </evidence>
    <sequence length="375" mass="42270" checksum="7856712EAA6EEBF0" modified="2020-08-12" version="1">MIFYMCLTLLAAFVACQASEEIYQILAKDYYASKNLIISPFAIDTILSLVYMGAAGATATELQNYLKLSSEDKQLVTSKYNEVLDGLFQPRADGPTLKLANRIYVNDRFRLKEEYNQVVRDAFHADAQMFSVAGGVDPTESINKWVQEQTNGKILNLLEPDSLKPDVMAVLLNAIYFKGEWQSKFDASNTQEGNFWVTGQETVPVQMMSQTGYFKARYFTDLQAQVIELPYLNSNLSMFLFLPKERDGLRAMEQKISGFSRHMHCMEAVVKLPKFKIEYGEDLKGTLQKLGIRELFTDDSDLSGLLADKANGKISKFAHKAVLDVNEEGAEASAATYGMITNRSSFTMVLSFDHPFAFVIRDQRTIYFQGHVVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3Y7</accession>
    <name>A0A6I8V3Y7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cell death-inducing p53-target protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC6898258</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <strain evidence="10 11">MV2-25</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138324.1">
        <property type="nucleotide sequence ID" value="XM_002138288.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039376.1">
        <property type="nucleotide sequence ID" value="XM_015183890.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3Y7"/>
    <dbReference type="GeneID" id="6898258"/>
    <dbReference type="KEGG" id="dpo:6898258"/>
    <dbReference type="OMA" id="QYCPNCK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246081">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF14">
        <property type="entry name" value="FI16615P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="9">
        <location>
            <begin position="55"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002138324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015039376.1"/>
        </source>
    </evidence>
    <sequence length="136" mass="15012" checksum="EAD16C2AFB5C62A4" modified="2020-08-12" version="1">MADYSKLGSPPAYMDEASAPPHTEMNQYAPQYDPQEIYPPLQPPAQVPPPQVAPPQVVHHTTTVVVNSNQRGMVCPQCNARIRVRTEFHPTGKTYCLAALLCLFLCWPCVCVPCCCNCCYKTSQFCPNCNACLGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4L4</accession>
    <name>A0A6I8V4L4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">trypsin</fullName>
            <ecNumber evidence="11">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6898785</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="11"/>
    <dbReference type="RefSeq" id="XP_002138762.1">
        <property type="nucleotide sequence ID" value="XM_002138726.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4L4"/>
    <dbReference type="GeneID" id="6898785"/>
    <dbReference type="KEGG" id="dpo:6898785"/>
    <dbReference type="InParanoid" id="A0A6I8V4L4"/>
    <dbReference type="OMA" id="NPNTMIN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246362">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026259974" description="trypsin" evidence="13">
        <location>
            <begin position="17"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="31"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002138762.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="25681" checksum="33A67FD23A3A18C9" modified="2020-08-12" version="1">MLKFVILLSAVACALGGTIPEGLLPQLDGRIVGGSATTISSFPWQISLQRSGSHSCGGSVYSANIIVTAAHCLQSVSASTLQVRAGSTYWSSGGTAVKVASFKNHEGYNANTMVNDIAVIRLSSSLGFSSTIKAIGLASSAPANGAAASVSGWGTQSSGSSSIPTTLQYVNVNIVGQSQCASSSYSYGSQIKSSMICAASSGKDACQGDSGGPLVSGGVLVGVVSWGYGCALSNYPGVYADVAALRSWVVNAANSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4R9</accession>
    <name>A0A6I8V4R9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138791.1">
        <property type="nucleotide sequence ID" value="XM_002138755.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4R9"/>
    <dbReference type="GeneID" id="6898817"/>
    <dbReference type="KEGG" id="dpo:6898817"/>
    <dbReference type="InParanoid" id="A0A6I8V4R9"/>
    <dbReference type="OMA" id="MQWLNLF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245596">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026109525" evidence="2">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138791.1"/>
        </source>
    </evidence>
    <sequence length="52" mass="5817" checksum="FEA45BB481CC87C0" modified="2020-08-12" version="1">MKWFSLFLVFGVLALIGSLGTLTAAEPVPAPEPKGRPHTTRRPRNDNDNDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7Z8</accession>
    <name>A0A6I8V7Z8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Electron transfer flavoprotein subunit alpha</fullName>
            <shortName evidence="7">Alpha-ETF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">wal</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7 8">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_004444503.1">
        <property type="nucleotide sequence ID" value="XM_004444446.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V7Z8"/>
    <dbReference type="SMR" id="A0A6I8V7Z8"/>
    <dbReference type="FunCoup" id="A0A6I8V7Z8">
        <property type="interactions" value="1276"/>
    </dbReference>
    <dbReference type="GeneID" id="4805402"/>
    <dbReference type="KEGG" id="dpo:4805402"/>
    <dbReference type="InParanoid" id="A0A6I8V7Z8"/>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081450">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045251">
        <property type="term" value="C:electron transfer flavoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000041">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 7 8" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="9">
        <location>
            <begin position="21"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="248"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="262"/>
            <end position="266"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="279"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_004444503.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="34270" checksum="F03D871392F3C658" modified="2020-08-12" version="1">MFASNTRNLMRSSFLQRCKSTLVVAEHNNEALNPITLNTISAAKKIGGDVTVLVAGTKCGPASEALAKVEGVTKVLVAENAAFKGFTPESLTPLVVAAQAQFKFTHILAGASAFGKNVLPRVAAKLDVSPISEVIDIKSEDTFVRTIYAGNAILTLKSKDPIKVLTVRSTNFQPAATSGGSGAVEQAAAGDYASSLSEFVSQELTKSDRPELAGAKVIISGGRGLKSGDNFKLLYDLADKFGAAVGASRAAVDAGFVPNDLQIGQTGKIVAPDLYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADIGIVADLFKAVPELTGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBB1</accession>
    <name>A0A6I8VBB1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Homeotic protein Sex combs reduced</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">Scr</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359213.4">
        <property type="nucleotide sequence ID" value="XM_001359176.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037706.2">
        <property type="nucleotide sequence ID" value="XM_015182220.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBB1"/>
    <dbReference type="KEGG" id="dpo:4802255"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000288">
        <property type="entry name" value="Sex combs reduced"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050609">
        <property type="entry name" value="Antp_homeobox_Deformed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771">
        <property type="entry name" value="HOMEOTIC PROTEIN DEFORMED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771:SF6">
        <property type="entry name" value="HOMEOTIC PROTEIN SEX COMBS REDUCED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="333"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="335"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="155"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="272"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001359213.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015037706.2"/>
        </source>
    </evidence>
    <sequence length="428" mass="45383" checksum="A789042BA1987EFC" modified="2020-08-12" version="1">MDPDCFAMSSYQFVNSLASCYPQQMNPQQSHPGANASAGSSNGAAVPGGGGTNGGPGNSGAATPGANDYFPAAAAYTPNLYPNTPQAHYANQAAAYGGQGNPDMVDYTQLQPQRLLLQQQQQQQQQQQQQQHAHAAAAVAAQQQQQLAQQQLPQQQQQQQQQTNISCKYANDPTTPGGSGGGGGGGGSNNNNNSSNNNNNQSLASPQDLSTRDISPKLSPSSVVESVARSLNKGVLGGSLAAAAAAVSLNNNHSGAGVGPVGGGGSGVGVGGVPGVVNVPMHSPGGGDSDSESDSGNEAGSSQNSGNGKKNPPQIYPWMKRVHLGQSTVNANGETKRQRTSYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLTERQIKIWFQNRRMKWKKEHKMASMNIVPYHMGPYGHPYHQFDIHPSQFAHLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBY2</accession>
    <name>A0A6I8VBY2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein takeout</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015038083.2">
        <property type="nucleotide sequence ID" value="XM_015182597.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBY2"/>
    <dbReference type="KEGG" id="dpo:26532184"/>
    <dbReference type="InParanoid" id="A0A6I8VBY2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038602">
        <property type="entry name" value="Mite_allergen_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020234">
        <property type="entry name" value="Mite_allergen_group-7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16984">
        <property type="entry name" value="Grp7_allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026244410" description="Protein takeout" evidence="1">
        <location>
            <begin position="23"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015038083.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="31072" checksum="05DD8D763B5BA1AC" modified="2020-08-12" version="1">MYVVRLIMCITFMAALVDVVTPRDEAKQEKSFRSHAFSKNASNEDDFEKDDIGWTNINEEVDVVTPMVLLHLQKLGIEHLQLPDMKESISIKPLFITYEAGLHLTNGIVYNLYGIARHGDAFMTYEGKSFLSRFYLKINKLQFEYNFFLKLMAIEGYGKVMGSLDDTVVYAELAVNVINSKLHLHDFRIMEFSNIHVQLDQARLIRQLTGIILSPITNFFKDRITTSIADGLKDQMQSVMDDFNNEDPLQLREFTKKLLSGITGNTPEDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDP8</accession>
    <name>A0A6I8VDP8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039136.1">
        <property type="nucleotide sequence ID" value="XM_015183650.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDP8"/>
    <dbReference type="GeneID" id="6898115"/>
    <dbReference type="InParanoid" id="A0A6I8VDP8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245927">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VDP8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.620">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015039136.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="22844" checksum="2D3C7EFF9829DE3E" modified="2020-08-12" version="1">MLCMPPATTDCPNVPLPVSGKTTRPEWNGVHPACRLDNCAVTKRQPAYQFSGQSGEGSDKALVCDGTTNCDNGHDEVDSRLSVSHQQVEWKATISAQILNQQYPTPPSHSFALAALLYDGWELNRWCERFVRYFYCRAALGPASDVLAHRAKESSSALMAPSKKINKVGPKAPCELVQQPSRIKVFVICIAGPRASSKPSAGVDLVEHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VE33</accession>
    <name>A0A6I8VE33_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039274.1">
        <property type="nucleotide sequence ID" value="XM_015183788.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VE33"/>
    <dbReference type="FunCoup" id="A0A6I8VE33">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="GeneID" id="26533621"/>
    <dbReference type="KEGG" id="dpo:26533621"/>
    <dbReference type="InParanoid" id="A0A6I8VE33"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272608">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031833">
        <property type="entry name" value="DUF4748"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15932">
        <property type="entry name" value="DUF4748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015039274.1"/>
        </source>
    </evidence>
    <sequence length="63" mass="7146" checksum="376A081E11C10C21" modified="2020-08-12" version="1">MTLGNAGRIAVCWGILTAGGVYAFVLSKQSVDSRRYESMRVRERMRKANHGDYDSSSSERRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VE89</accession>
    <name>A0A6I8VE89_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015038943.1">
        <property type="nucleotide sequence ID" value="XM_015183457.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VE89"/>
    <dbReference type="GeneID" id="26532139"/>
    <dbReference type="KEGG" id="dpo:26532139"/>
    <dbReference type="InParanoid" id="A0A6I8VE89"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271126">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015038943.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="37538" checksum="9CC273457DAE38C1" modified="2020-08-12" version="1">MVVGSGLHRSKPKGKASSCGAINRLPLRTSGISAIDSTALNARRISRNPRSAAGALALGGRIAGSLGAATMGINTGRVSTLGIGGAGTGAQTPKLLSANGQEFQRSPPLRRGTVSAVHTPLTSVAKVPSAKTCSSASAPNIVSVHSALKKSSLSRKVGGGKVIDASAKRRVKFSANVHTFPLTPTKKSSRIGELQLKKRVKKLKRNRTRKEGGSGILSEGRVGFGLSSIGIAGSRSGHDVKQPGNIVNVQAAPRIPRKRLAAASGSRAHPISVKKRIATPPSAALYNTRYPSGSVILSAAKERTSPAKGQANSKLRTATPSRLGHTPVAGVHRRKTTPETSPKSAHRRSKSHGQVRGGRATGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VEK0</accession>
    <name>A0A6I8VEK0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cathepsin L1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CtsK2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015039445.2">
        <property type="nucleotide sequence ID" value="XM_015183959.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VEK0"/>
    <dbReference type="FunCoup" id="A0A6I8VEK0">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803937"/>
    <dbReference type="InParanoid" id="A0A6I8VEK0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VEK0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000109">
        <property type="entry name" value="Cysteine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="9">
        <location>
            <begin position="96"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="8">
        <location>
            <begin position="188"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015039445.2"/>
        </source>
    </evidence>
    <sequence length="404" mass="43106" checksum="6C97D1DA932F9192" modified="2020-08-12" version="1">MIAPYQIGGYKSPSFGSPAIPLVLVDRSRKITMKFLLVLPLFVALASAQFGGFGGGLGGNRLGGALSNAANRVQQGIANIARQLPSPPKITNVQNFGDFLAQSGKNYLSAADKALHEGVFAARKNLVDAGNDAFAKGASSYQLAVNAFSDLTKSEFLSQLTGLRKSSQGASKATANRKLASVPAGASIPESFDWRQKGGVTSVKFQGTCGSCWAFATTGAIEGHIFRKTGTLPNLSEQNLVDCGTLEFGLSGCDGGFQEYAMAFINEEQKGVSKADGYPYIDNKDTCKYSKNLSGAQITGFATIPPKDETLMKKVIATLGPLACSLNGLETLLQYKSGIYSDEKCNEGEPNHSVLVVGYGSEKGQDYWIVKNSWDKVWGEEGYFRLPRGNNFCGIALECTYPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHH5</accession>
    <name>A0A6I8VHH5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6901213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041482.2">
        <property type="nucleotide sequence ID" value="XM_015185996.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VHH5"/>
    <dbReference type="KEGG" id="dpo:6901213"/>
    <dbReference type="InParanoid" id="A0A6I8VHH5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015041482.2"/>
        </source>
    </evidence>
    <sequence length="147" mass="17042" checksum="0D1016B179B0A483" modified="2020-08-12" version="1">MARVLPEKNNAAHTMNGHHRKRKWQARRGEDLTPDDRIELIAELMAKLDGVNFVADFFRIRRLKLLITDCLPDHKEQLLRILIGYVNRPPSPATASYARIVVLLSNDFYLLMVDCIRALMEVYQMALIDGKWQNARGLECFFSELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VII9</accession>
    <name>A0A6I8VII9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Zinc finger protein 311 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC6901387</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041657.2">
        <property type="nucleotide sequence ID" value="XM_015186171.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VII9"/>
    <dbReference type="FunCoup" id="A0A6I8VII9">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VII9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VII9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001792">
        <property type="entry name" value="Zinc finger and BTB domain-containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000663">
        <property type="entry name" value="Zinc finger protein 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF15">
        <property type="entry name" value="IP01243P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="298"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015041657.2"/>
        </source>
    </evidence>
    <sequence length="352" mass="40074" checksum="9AD90B2914A849C2" modified="2020-08-12" version="1">MFKVQINNIYRGRAANIMGQPTIIPSTQLVSIAAAEQEMTALAVLARQREDEQANYSVCLEDAQLFGQLDELRGDEDTEDMELMMLSPGADFDLIDLVNGALDPTGNHHHSNTRLQSKPKRTAKQGGPQGAPHACDVCGRRFSEAYNLRIHKMTHTDEKPHVCDGCGKGFRQLNKLRIHYVTHTPDRPHKCDICGKGFRFANYLVVHRRLHTGEKPYSCSVEECDLTFHSIHARRIHTKLQHAAPVQSDAEQEQPETTTATSSSTSSLCYTCTICGRVLTDQCYLNTHMKRHYNQRDFPCPQPECGKRFFSSSELKHHQIAHTQYRPYACTECPARFLRKSNYKQHLKIHER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJT8</accession>
    <name>A0A6I8VJT8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">trypsin</fullName>
            <ecNumber evidence="11">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC26533666</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="11"/>
    <dbReference type="RefSeq" id="XP_015042864.2">
        <property type="nucleotide sequence ID" value="XM_015187378.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VJT8"/>
    <dbReference type="SMR" id="A0A6I8VJT8"/>
    <dbReference type="FunCoup" id="A0A6I8VJT8">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26533666"/>
    <dbReference type="InParanoid" id="A0A6I8VJT8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000034">
        <property type="entry name" value="Eupolytin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026144434" description="trypsin" evidence="13">
        <location>
            <begin position="16"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="23"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015042864.2"/>
        </source>
    </evidence>
    <sequence length="254" mass="27084" checksum="96F1A9A6553E4059" modified="2020-08-12" version="1">MEWLWLFCIIQTAMAVARSGPRIVGGKETSIAQVPYLVYLRQGGFFLCGGSLLSPTVVLSAAHCVYGAQPEDYTVHAGASRLDQASPVVRGVASFHLSPSYSSTNFDMDVALLQLRESVPLSAGSVATIAACRMPPDSNAYARISGWGVTRENNREPAVQVRTAMVRMLPGAECRLSYAGVAKISDSMICASVRGLRDSCSGDSGGPLVYRGQVCGIVSWGFGCARLAYPGVYTSVASVRVRSFIERTLQEIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKV9</accession>
    <name>A0A6I8VKV9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4813692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015043041.2">
        <property type="nucleotide sequence ID" value="XM_015187555.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VKV9"/>
    <dbReference type="KEGG" id="dpo:4813692"/>
    <dbReference type="InParanoid" id="A0A6I8VKV9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VKV9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019991">
        <property type="term" value="P:septate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056677">
        <property type="entry name" value="DUF7775"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038976">
        <property type="entry name" value="Ssk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36692:SF1">
        <property type="entry name" value="GH08457P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36692">
        <property type="entry name" value="PROTEIN SNAKESKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24985">
        <property type="entry name" value="DUF7775"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7775" evidence="2">
        <location>
            <begin position="36"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015043041.2"/>
        </source>
    </evidence>
    <sequence length="175" mass="19630" checksum="7ECBA657A67A8C0C" modified="2020-08-12" version="1">MSYLRSIEISRVEMSPADDNSFATTSGNKKATYTLICIKVVELCLLICCLGLIDEPATNSHLRVFITPRVIALCYVTFGALTIYTAIYLIMALVGDLTPWRTATLWSLVAFCLYVAVTALLFRDWSTTKDRNYWHPNMHRLDLVMASASIALVTSLVYLLDILLTIRFGVHGDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VN73</accession>
    <name>A0A6I8VN73_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">28S ribosomal protein S7, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mRpS7</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015044386.2">
        <property type="nucleotide sequence ID" value="XM_015188900.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VN73"/>
    <dbReference type="FunCoup" id="A0A6I8VN73">
        <property type="interactions" value="640"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26533670"/>
    <dbReference type="InParanoid" id="A0A6I8VN73"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VN73">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14870">
        <property type="entry name" value="uS7_Mitochondria_Mammalian"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="4">
        <location>
            <begin position="58"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015044386.2"/>
        </source>
    </evidence>
    <sequence length="217" mass="25114" checksum="7A0D76530F794FA8" modified="2020-08-12" version="1">MAVFYRIAIQSSIYGCRSMSMFPKHFGDPLRKKDMEDLNKSELTKFYHIPIKAALNNHSDTVFSDEIKLKMMNYITKNGNKALARGLLSRTFEIIKRAQTERLHLSPTDKQNVNTNAEKLLIEAVENSRPLLQLTSIKRGGVNYQVPVPITKKRSYFLAMKWLLDAAFEKDKKVCLPERLAWEILDAAHGQGRVIKKKDDVHKQCESNRAYAHYRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VT06</accession>
    <name>A0A6I8VT06_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4744 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117183658</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234192.1">
        <property type="nucleotide sequence ID" value="XM_033378301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VT06"/>
    <dbReference type="KEGG" id="dpo:117183658"/>
    <dbReference type="InParanoid" id="A0A6I8VT06"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031806">
        <property type="entry name" value="DUF4744"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15918">
        <property type="entry name" value="DUF4744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4744" evidence="2">
        <location>
            <begin position="67"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234192.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="13207" checksum="C3D2E077B584713E" modified="2020-08-12" version="1">MMPLLCRWVDNGVPSSAADLGKSVSNLPIHTLLNQTLLQLRDIVISDFPPPNEVLSKNYRGVFETDRVPSQADRHVSQQDRSSPLARSEPNKPSSSPHLAIDNKDFDYGNQWRGYAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VUQ7</accession>
    <name>A0A6I8VUQ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cardioactive peptide</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCAP</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234807.1">
        <property type="nucleotide sequence ID" value="XM_033378916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VUQ7"/>
    <dbReference type="FunCoup" id="A0A6I8VUQ7">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800944"/>
    <dbReference type="InParanoid" id="A0A6I8VUQ7"/>
    <dbReference type="OMA" id="DKPYNNE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024276">
        <property type="entry name" value="CCAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11105">
        <property type="entry name" value="CCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026314357" evidence="1">
        <location>
            <begin position="24"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234807.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="18134" checksum="9A048AC9B14DC15B" modified="2020-08-12" version="1">MKSSLKMCLGLVVLLICMLSGNASLERENNEVNNLANHKLSGVIQWKYEKRPFCNAFTGCGRKRTSYPSYPPFSLIKRNELEEKPYNNEYLSEGLSDLIDINAEPAVENVQKQIMSQAKIFEAIKEASKEIFRQKSNRQKMLENGQRLQMQEERENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VUW3</accession>
    <name>A0A6I8VUW3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234872.1">
        <property type="nucleotide sequence ID" value="XM_033378981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VUW3"/>
    <dbReference type="KEGG" id="dpo:117183767"/>
    <dbReference type="InParanoid" id="A0A6I8VUW3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026060351" evidence="1">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234872.1"/>
        </source>
    </evidence>
    <sequence length="67" mass="7720" checksum="2CA5C77479B41E58" modified="2020-08-12" version="1">MHRFQFVGFLLLQLLLQGYMYSHRHPGFTQRLQINPLCLELQQRTTPASGAAEIIFSPLEEDTTEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXS3</accession>
    <name>A0A6I8VXS3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC6903093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235852.1">
        <property type="nucleotide sequence ID" value="XM_033379961.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235853.1">
        <property type="nucleotide sequence ID" value="XM_033379962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXS3"/>
    <dbReference type="KEGG" id="dpo:6903093"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VXS3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235853.1"/>
        </source>
    </evidence>
    <sequence length="524" mass="57219" checksum="9DBEC823CE5FF4E2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZQ3</accession>
    <name>A0A6I8VZQ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein pim isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pim</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236576.1">
        <property type="nucleotide sequence ID" value="XM_033380685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZQ3"/>
    <dbReference type="FunCoup" id="A0A6I8VZQ3">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VZQ3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VZQ3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033236576.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="26557" checksum="19B2A0FC53F90BA2" modified="2020-08-12" version="1">MEQIFLAKENPVSSISIKKNALSINNSSIKQNARKLVLGKLENVMHQNQTPFKSDSISFPKSVAKLPLQNFHRNYDNERGEAILKVTPNYLGFYSLKNEICSQSAYENLFDYMDFSSTKCLNKCWRPLLFVANDHFAKGGRHVHSYLTIAEIWLDNQSGRTTFTQTILNDLGDLVETLETECCISIYNVFCDNFDRDNCSKQDIDNTGTEQCEYKLDSHMALPNLDDIDIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3T1</accession>
    <name>A0A6I8W3T1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237354.1">
        <property type="nucleotide sequence ID" value="XM_033381463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W3T1"/>
    <dbReference type="KEGG" id="dpo:26533111"/>
    <dbReference type="InParanoid" id="A0A6I8W3T1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272098">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033237354.1"/>
        </source>
    </evidence>
    <sequence length="125" mass="14304" checksum="FF1FDF42C8FE0CF1" modified="2020-08-12" version="1">MCAKQCDDRAVSIVLGAWNLISGPCWFLLSVYFISQRKKPEDGTIVSYEKFWYVFCMIVFVAAIIHTLSGVLLLFGVWKDYGGVFLVGMVISNFLPIVLVLTIIIPIIQVFCVFRALNYWRSNWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4V1</accession>
    <accession>Q29G34</accession>
    <name>A0A6I8W4V1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Transcription factor HES-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">Hesr</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033238376.1">
        <property type="nucleotide sequence ID" value="XM_033382485.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238377.1">
        <property type="nucleotide sequence ID" value="XM_033382486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4V1"/>
    <dbReference type="SMR" id="A0A6I8W4V1"/>
    <dbReference type="KEGG" id="dpo:4814220"/>
    <dbReference type="OMA" id="ERYWSGF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="89"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033238376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033238377.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="24295" checksum="AA5B7BB35C495477" modified="2020-08-12" version="1">MQPRLLSEWLSEIGADLSEKRCSASATASATAATATWALTVSLCKNGVQFGAKSTATLAKSTYIPNEMKRNQIFRGGYVEQGLSRSSEYRKVMKPLLERKRRARINNCLDELKIIIADLAHMETTGLNKLEKADILELAVHLLQEQRACSSSQLSGPVAHQQRLEAESYWGGFRQCAVQVSSFLQHHDKALSDQFNEFLQQILPAKPAPEPPLWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5M1</accession>
    <name>A0A6I8W5M1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238582.1">
        <property type="nucleotide sequence ID" value="XM_033382691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5M1"/>
    <dbReference type="FunCoup" id="A0A6I8W5M1">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184537"/>
    <dbReference type="InParanoid" id="A0A6I8W5M1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21391">
        <property type="entry name" value="AT04489P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21391:SF0">
        <property type="entry name" value="AT04489P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="587" mass="68774" checksum="E3C1A04ECD7524D0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6W4</accession>
    <name>A0A6I8W6W4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">GTP-binding protein Rhes</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">LOC4813473</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239047.1">
        <property type="nucleotide sequence ID" value="XM_033383156.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239048.1">
        <property type="nucleotide sequence ID" value="XM_033383157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6W4"/>
    <dbReference type="KEGG" id="dpo:4813473"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081219">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W6W4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04143">
        <property type="entry name" value="Rhes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000475">
        <property type="entry name" value="GTP-binding protein Rhes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF3">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="28"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033239047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033239048.1"/>
        </source>
    </evidence>
    <sequence length="449" mass="48569" checksum="18EB78C4CFC51D0B" modified="2020-08-12" version="1">MYRCKNWFCARSNNNNYVDVALSEPGTLPAPSSATSTGTTTANSFGIGNARNNNNSSSGSGSGSGRIKSNQQTNATAAPNRATPTADEASSPQAGAGAGAGAAAGAGPGAAHVVTFLDDTASGSNGTVTSSRIVTTAELHQAHMLEHHSNLDAIEQADDFIYGPGAGLSLCDDSLPSAKNCYRLVMLGSSRAGKSSIVARFLGNRFEEAYTPTIEEFHRKLYRIRNEVFQLDILDTSGYHPFPAMRRLSFLTGDLFILVFSMDSRESFEEVVRLRENILETKWAALNPGSGFKKKSLPKIPMILAGNKCDRDFKTVQLDEVMGYIAGQDNCCTFVECSARQNYRIDDLFYALFMVSNLPLEMTPNHHRRLVSVFGAPSPLPPHGSAVGGTKKNALSIKRRFSDACGVVTPNARRPSIRTDLNLMRSKTMALNEGETVRTTSRWNRCALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6Z6</accession>
    <name>A0A6I8W6Z6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117184644</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239077.1">
        <property type="nucleotide sequence ID" value="XM_033383186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6Z6"/>
    <dbReference type="KEGG" id="dpo:117184644"/>
    <dbReference type="InParanoid" id="A0A6I8W6Z6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="3">
        <location>
            <begin position="334"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="266"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239077.1"/>
        </source>
    </evidence>
    <sequence length="800" mass="89733" checksum="C858BD05D2C47936" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7J3</accession>
    <name>A0A6I8W7J3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Protein mab-21-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC5614322</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mab-21 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354847.1">
        <property type="nucleotide sequence ID" value="XM_001354811.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239345.1">
        <property type="nucleotide sequence ID" value="XM_033383454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W7J3"/>
    <dbReference type="SMR" id="A0A6I8W7J3"/>
    <dbReference type="GeneID" id="5614322"/>
    <dbReference type="KEGG" id="dpo:5614322"/>
    <dbReference type="OMA" id="WDESCIA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078417">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W7J3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000002">
        <property type="entry name" value="protein mab-21-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.90:FF:000001">
        <property type="entry name" value="protein mab-21-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF70">
        <property type="entry name" value="PROTEIN MAB-21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mab-21-like nucleotidyltransferase" evidence="2">
        <location>
            <begin position="69"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="3">
        <location>
            <begin position="255"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033239345.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="42451" checksum="18268C1041FAB25B" modified="2020-08-12" version="1">MLVPPDMLVAQTRMIYQMNRYCAERVQARMFKTSTAIREICKIVQDILKEVELQEPRFISSLVECNGRFEGVEVISPNEFEIVLYLNQMGVFNFVDDGTLPGCAVLKLSDGRKRSMSLWVEFITASGYLSSRKIRSRFQTLVAQACDKSMYRDMVKIIGDTTEVKLRIRERYVVQITPAFKCSGIWPRSAAHWPMPHIPWPHPNIVAEVKTEGFDLLSKDSAAMQNKNNNAASMEGDAWVLSFYEAENRLLQGGCRRRCLSMLKTLRDRHLELPGSPISAYHLKNLLLYECEKHPRDYEWDESCIADRINGIFLQLISCLQYRRCPHYFLPSLDMFKGKSPSALEQAAKQVWRLTREMLTNANAFEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8N1</accession>
    <name>A0A6I8W8N1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18 19">Fibrillin-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18 19" type="primary">LOC6899990</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17 18 19">MV-25-SWS-2005</strain>
            <tissue evidence="17 18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135356.3">
        <property type="nucleotide sequence ID" value="XM_002135320.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238979.1">
        <property type="nucleotide sequence ID" value="XM_033383088.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238980.1">
        <property type="nucleotide sequence ID" value="XM_033383089.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899990"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W8N1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000847">
        <property type="entry name" value="Faulty attraction, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000897">
        <property type="entry name" value="Faulty attraction, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000037">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000008">
        <property type="entry name" value="Signal peptide, CUB domain, EGF-like 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050751">
        <property type="entry name" value="ECM_structural_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034:SF200">
        <property type="entry name" value="EGF-LIKE AND EMI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634628" evidence="12">
        <location>
            <begin position="20"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="22"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="373"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="603"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="638"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="718"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="762"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="830"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="1032"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="1073"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="1222"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="14">
        <location>
            <begin position="1373"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="14">
        <location>
            <begin position="1567"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="201"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1489"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1651"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1243"/>
            <end position="1270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002135356.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033238979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033238980.1"/>
        </source>
    </evidence>
    <sequence length="1749" mass="190308" checksum="4D18A2A8E3C6CD18" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8N9</accession>
    <name>A0A6I8W8N9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MOB kinase activator-like 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mob as tumor suppressor protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Mps one binder kinase activator-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Mob2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the normal morphogenesis of a variety of polarized outgrowths including epidermal hairs, bristles, arista laterals, and dendrites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with and activates trc, also interacts with wts.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MOB1/phocein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239743.1">
        <property type="nucleotide sequence ID" value="XM_033383852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W8N9"/>
    <dbReference type="FunCoup" id="A0A6I8W8N9">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W8N9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000003">
        <property type="entry name" value="MOB kinase activator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0691">RNA editing</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="244"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="511"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="685"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="761"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="244"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="512"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="555"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="699"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="770"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033239743.1"/>
        </source>
    </evidence>
    <sequence length="785" mass="82853" checksum="BB474F55B99A6F57" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9A7</accession>
    <name>A0A6I8W9A7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Prominin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC117184687</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239194.1">
        <property type="nucleotide sequence ID" value="XM_033383303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9A7"/>
    <dbReference type="KEGG" id="dpo:117184687"/>
    <dbReference type="InParanoid" id="A0A6I8W9A7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF1">
        <property type="entry name" value="PROMININ-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033239194.1"/>
        </source>
    </evidence>
    <sequence length="75" mass="8246" checksum="B79C1641215782EB" modified="2020-08-12" version="1">MTNVGRCQPLAYIYYQGVHSVCARLVDPINAFWLATIICALLLLPTLFVCHRLMCLYLRIYPDSVDDAAGGVVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCD1</accession>
    <name>A0A6I8WCD1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Coiled-coil domain-containing protein CG32809 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6902076</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240219.1">
        <property type="nucleotide sequence ID" value="XM_033384328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCD1"/>
    <dbReference type="FunCoup" id="A0A6I8WCD1">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WCD1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WCD1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF10">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN CG32809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3-like C-terminal" evidence="4">
        <location>
            <begin position="173"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Actin interacting protein 3-like C-terminal" evidence="4">
        <location>
            <begin position="586"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="530"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="791"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="851"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="969"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="673"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="854"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="879"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="982"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1036"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033240219.1"/>
        </source>
    </evidence>
    <sequence length="1254" mass="137269" checksum="C1A9DBCE9767E559" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDT4</accession>
    <name>A0A6I8WDT4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117185079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240724.1">
        <property type="nucleotide sequence ID" value="XM_033384833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDT4"/>
    <dbReference type="KEGG" id="dpo:117185079"/>
    <dbReference type="InParanoid" id="A0A6I8WDT4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22954:SF3">
        <property type="entry name" value="PROTEIN CBG08539"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22954">
        <property type="entry name" value="RETROVIRAL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033240724.1"/>
        </source>
    </evidence>
    <sequence length="347" mass="39313" checksum="F7228AF6286E4F53" modified="2020-08-12" version="1">MTSEQNRVSFLKLKSTEFCEKMKRLATAWQNESSDCDYYMLVVKQEQVEKLIGKFEMFHGELEELDRSEIHSGLCDIFQSLASSLKAANMREMAKSSGRMPFHSTSDGGNTTVEFAGSQFLPHRRQVPSLPPIQLPTFSGGYANWTEFYSMLAIIIDSDPDLTNIEKLQHLRSCLRDSALETVRALEISDGNYAVALDLLENRFNNRRLVFQAHINEILALQAVEPGSVVMLRELSDKFNSHMRALQGLGTTEQIAGCIVVQELLRKLDPPSQEKWEERFIDLAFANLIPTWESMASFLEQRCRSLETMDFSMASYASSSQVGRSRKHNNSRSTLVTMNQSVARCAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>B5DV17</accession>
    <accession>A0A6I8W5G3</accession>
    <name>B5DV17_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 10">ERI1 exoribonuclease 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Dpse\GA23276</name>
        <name evidence="6" type="synonym">Dpse\GA23279</name>
        <name evidence="9" type="synonym">LOC117184524</name>
        <name evidence="10" type="synonym">LOC117184525</name>
        <name evidence="11" type="synonym">LOC117184528</name>
        <name evidence="8" type="synonym">LOC6903743</name>
        <name evidence="5" type="ORF">Dpse_GA23276</name>
        <name evidence="6" type="ORF">Dpse_GA23279</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
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                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
                <person name="Castrezana S."/>
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                <person name="Chang J.L."/>
                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
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                <person name="Costello J.C."/>
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                <person name="Freyhult E."/>
                <person name="Fulton L."/>
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                <person name="Gotea V."/>
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                <person name="Griffiths-Jones S."/>
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                <person name="Haerty W."/>
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                <person name="Holmes I."/>
                <person name="Hoskins R.A."/>
                <person name="Hubisz M.J."/>
                <person name="Hultmark D."/>
                <person name="Huntley M.A."/>
                <person name="Jaffe D.B."/>
                <person name="Jagadeeshan S."/>
                <person name="Jeck W.R."/>
                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
                <person name="Li R."/>
                <person name="Lin C.F."/>
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                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
                <person name="Machado C.A."/>
                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
                <person name="O'Grady P."/>
                <person name="Pachter L."/>
                <person name="Papaceit M."/>
                <person name="Parisi M.J."/>
                <person name="Parisi M."/>
                <person name="Parts L."/>
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                <person name="Pesole G."/>
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                <person name="Ponting C.P."/>
                <person name="Pop M."/>
                <person name="Porcelli D."/>
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                <person name="Prohaska S."/>
                <person name="Pruitt K."/>
                <person name="Puig M."/>
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                <person name="Ram K.R."/>
                <person name="Rand D."/>
                <person name="Rasmussen M.D."/>
                <person name="Reed L.K."/>
                <person name="Reenan R."/>
                <person name="Reily A."/>
                <person name="Remington K.A."/>
                <person name="Rieger T.T."/>
                <person name="Ritchie M.G."/>
                <person name="Robin C."/>
                <person name="Rogers Y.H."/>
                <person name="Rohde C."/>
                <person name="Rozas J."/>
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                <person name="Ruiz A."/>
                <person name="Russo S."/>
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                <person name="Sanchez-Gracia A."/>
                <person name="Saranga D.J."/>
                <person name="Sato H."/>
                <person name="Schaeffer S.W."/>
                <person name="Schatz M.C."/>
                <person name="Schlenke T."/>
                <person name="Schwartz R."/>
                <person name="Segarra C."/>
                <person name="Singh R.S."/>
                <person name="Sirot L."/>
                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
                <person name="Smith T.F."/>
                <person name="Spieth J."/>
                <person name="Stage D.E."/>
                <person name="Stark A."/>
                <person name="Stephan W."/>
                <person name="Strausberg R.L."/>
                <person name="Strempel S."/>
                <person name="Sturgill D."/>
                <person name="Sutton G."/>
                <person name="Sutton G.G."/>
                <person name="Tao W."/>
                <person name="Teichmann S."/>
                <person name="Tobari Y.N."/>
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                <person name="Tsolas J.M."/>
                <person name="Valente V.L."/>
                <person name="Venter E."/>
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                <person name="Vicario S."/>
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                <person name="Villasante A."/>
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                <person name="Wilson R.K."/>
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                <person name="Wolfner M.F."/>
                <person name="Wong A."/>
                <person name="Wong G.K."/>
                <person name="Wu C.I."/>
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                <person name="Zdobnov E."/>
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                <person name="Abdouelleil A."/>
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                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
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                <person name="D'Aco K."/>
                <person name="Daza R."/>
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                <person name="DeMaso C."/>
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                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
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                <person name="Dupes A."/>
                <person name="Elong R."/>
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                <person name="Hollinger A."/>
                <person name="Honan T."/>
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                <person name="Hurhula B."/>
                <person name="Husby M.E."/>
                <person name="Kamat A."/>
                <person name="Kanga B."/>
                <person name="Kashin S."/>
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                <person name="Liu X."/>
                <person name="Liu J."/>
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                <person name="Lokyitsang T."/>
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                <person name="Lubonja R."/>
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                <person name="Montmayeur A."/>
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                <person name="Nicol R."/>
                <person name="Norbu C."/>
                <person name="Norbu N."/>
                <person name="Novod N."/>
                <person name="O'Neill B."/>
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                <person name="Oyono O.L."/>
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                <person name="Rege F."/>
                <person name="Reyes R."/>
                <person name="Rise C."/>
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                <person name="Settipalli S."/>
                <person name="Shea T."/>
                <person name="Sherpa N."/>
                <person name="Shi L."/>
                <person name="Shih D."/>
                <person name="Sparrow T."/>
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                <person name="Tesfaye S."/>
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                <person name="Brockman W."/>
                <person name="Butler J."/>
                <person name="Chin C."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Kleber M."/>
                <person name="Mauceli E."/>
                <person name="MacCallum I."/>
            </authorList>
            <dbReference type="PubMed" id="17994087"/>
            <dbReference type="DOI" id="10.1038/nature06341"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
            <authorList>
                <person name="English A.C."/>
                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9 10">MV-25-SWS-2005</strain>
            <tissue evidence="8 9 10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CH675205">
        <property type="protein sequence ID" value="EDY71749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CH675205">
        <property type="protein sequence ID" value="EDY71752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002136732.1">
        <property type="nucleotide sequence ID" value="XM_002136696.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238526.1">
        <property type="nucleotide sequence ID" value="XM_033382635.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238527.1">
        <property type="nucleotide sequence ID" value="XM_033382636.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238531.1">
        <property type="nucleotide sequence ID" value="XM_033382640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DV17"/>
    <dbReference type="SMR" id="B5DV17"/>
    <dbReference type="GeneID" id="6903743"/>
    <dbReference type="KEGG" id="dpo:117184524"/>
    <dbReference type="KEGG" id="dpo:117184525"/>
    <dbReference type="KEGG" id="dpo:117184528"/>
    <dbReference type="KEGG" id="dpo:6903743"/>
    <dbReference type="eggNOG" id="KOG0542">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037266_4_0_1"/>
    <dbReference type="OMA" id="SYRRNQI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244678">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06133">
        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="4">
        <location>
            <begin position="6"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EDY71749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EDY71752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002136732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033238526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033238527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238531.1"/>
        </source>
    </evidence>
    <sequence length="223" mass="25717" checksum="9C0ECD45950B117D" modified="2008-10-14" version="1">MHPFTYAISVDFEATCWVNQPAQQFRLSEIIEFPAILVNLKTGMVEAEFHKYVMPVEKPQLSEYCTSLTGIQQKTVEAGVPLQTALNSFIEWLKKELSARNLVLPKMSMTNPQGNCFFVTWTNWDFGICLAKECARKNIRKPTCFNQWIDAKAIYQKWYKYRPFSFNNALEHVRLTFQGRAHSGIDDAKNLGSLICKMFRDGAPFSITMDLTPHKELNENRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>B5DVA8</accession>
    <accession>A0A6I8VZ32</accession>
    <name>B5DVA8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-ribosomal protein eS31 fusion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RpS27A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235779.1">
        <property type="nucleotide sequence ID" value="XM_033379888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DVA8"/>
    <dbReference type="SMR" id="B5DVA8"/>
    <dbReference type="FunCoup" id="B5DVA8">
        <property type="interactions" value="1842"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DVA8"/>
    <dbReference type="KEGG" id="dpo:6903648"/>
    <dbReference type="eggNOG" id="KOG0004">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_2_0_1"/>
    <dbReference type="InParanoid" id="B5DVA8"/>
    <dbReference type="OMA" id="GVFMAFH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245617">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033235779.1"/>
        </source>
    </evidence>
    <sequence length="156" mass="17940" checksum="4FB9AC25B8E86EF5" modified="2008-10-14" version="1">MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKNYSTPKKIKHKRKKVKLAVLKYYKVDENGKIHRLRRECPGENCGAGVFMAAHEDRHYCGKCNLTFVFSKPEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-09-05" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I5AMQ1</accession>
    <accession>A0A6I8V540</accession>
    <name>I5AMQ1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Atg101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736163.1">
        <property type="nucleotide sequence ID" value="XM_003736115.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I5AMQ1"/>
    <dbReference type="SMR" id="I5AMQ1"/>
    <dbReference type="FunCoup" id="I5AMQ1">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="46245.I5AMQ1"/>
    <dbReference type="GeneID" id="4814260"/>
    <dbReference type="KEGG" id="dpo:4814260"/>
    <dbReference type="eggNOG" id="KOG4493">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110397_0_0_1"/>
    <dbReference type="InParanoid" id="I5AMQ1"/>
    <dbReference type="OMA" id="TMNCRSE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080058">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I5AMQ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_003736163.1"/>
        </source>
    </evidence>
    <sequence length="218" mass="25098" checksum="B8D5E0583952C15F" modified="2012-09-05" version="1">MNARSQVFDLTMEGLQVDEAVTTIFQTVLFHRCLGKFMYTGDAQYSIGTVGYTDVDCNFIDFTYVCCTSDSLTHKVKRAINLFSEKLRSNESCGSGQISLEFFQKKKNRWPFSQESIPWEVWTVHLDLIKHENEDERQLCRENVSDLLTEKVIYITELMNRHDYVPKTPSQSELDLIFDTSFPDVQPYLFKFDYSTSGSAAPSMGNAMKKIIKETLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EH7</accession>
    <accession>A0A6I8UAX1</accession>
    <name>Q29EH7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4812569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352586.3">
        <property type="nucleotide sequence ID" value="XM_001352550.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EH7"/>
    <dbReference type="GeneID" id="4812569"/>
    <dbReference type="KEGG" id="dpo:4812569"/>
    <dbReference type="eggNOG" id="ENOG502T8GF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097306_0_0_1"/>
    <dbReference type="InParanoid" id="Q29EH7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072036">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004019">
        <property type="entry name" value="YLP_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02757">
        <property type="entry name" value="YLP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001352586.3"/>
        </source>
    </evidence>
    <sequence length="259" mass="29195" checksum="203CDB60AF856B90" modified="2016-01-20" version="3">MYVMSHGTSICPLEPEAPVKRFQQQTAIRAEQPNAQQIEQVRTRYKTEKYTIKMQGVRFGIVCALLLAVGVSCRSVVRRDVSHLPLEYLPPVVLPPVPSRDYLPPVEEHAATLNHVLQPPRDYLPPVNNEYLPPVVEEELQEAPVVTTEEEIAVETTAAPTTEAVTEAQTEPEPEPEAEIVTEPVEVKALEHIEESAVLLDDGYHYRTPQVVPNLLPVKEYLPPLDDNAVVEEIPEHEEDSSALLDDGYHYRSVKRLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EY9</accession>
    <accession>A0A6I8UAI2</accession>
    <name>Q29EY9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812622</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352424.2">
        <property type="nucleotide sequence ID" value="XM_001352388.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EY9"/>
    <dbReference type="STRING" id="46245.Q29EY9"/>
    <dbReference type="GeneID" id="4812622"/>
    <dbReference type="KEGG" id="dpo:4812622"/>
    <dbReference type="eggNOG" id="ENOG502SGR0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159526_0_0_1"/>
    <dbReference type="InParanoid" id="Q29EY9"/>
    <dbReference type="OMA" id="MAKNTHI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073441">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EY9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043511961" description="Secreted protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352424.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="12838" checksum="8BD3A35CE10F9858" modified="2008-10-14" version="2">MAKNTHIAAAVALLLLALCTFIQAAPYQELEPRKGHVPVYIRHGNEPLSEIHPGLAEAFKEGQSKALVAESLVTEIPEAETSRPASGDAPSTTLAPSNEKSSTESDIASFKTIKATEPAME</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EZ5</accession>
    <accession>A0A6I8UAJ5</accession>
    <name>Q29EZ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vacuolar fusion protein CCZ1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Ccz1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCZ1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352418.1">
        <property type="nucleotide sequence ID" value="XM_001352382.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EZ5"/>
    <dbReference type="SMR" id="Q29EZ5"/>
    <dbReference type="FunCoup" id="Q29EZ5">
        <property type="interactions" value="2690"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EZ5"/>
    <dbReference type="GeneID" id="4811977"/>
    <dbReference type="KEGG" id="dpo:4811977"/>
    <dbReference type="eggNOG" id="KOG2622">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037828_2_0_1"/>
    <dbReference type="InParanoid" id="Q29EZ5"/>
    <dbReference type="OMA" id="DCQALHT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073438">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EZ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043988">
        <property type="entry name" value="CCZ1/INTU_longin_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19032">
        <property type="entry name" value="Intu_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="2">
        <location>
            <begin position="13"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU second Longin" evidence="3">
        <location>
            <begin position="212"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HPS4 third Longin" evidence="4">
        <location>
            <begin position="375"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352418.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="54574" checksum="2CA90FB2A09D18B6" modified="2006-04-04" version="1">MTKLLQRLEVTLRSFYVFNSSFGEKEGEEHKKVLYYHPNDIELNTKIKDVGLSEAIIRFTGTFTTEDDCKALHTQKTTQLFHQPELGFWLVLVLNVPKEIRLKEGVEVADYRGGEISDRIYRAVLRQCYQMYRFQHGALASLGAGLDNADQRREMLSEKLLQFFDTYLISLKDLAHCDIIDMLHSIQYLPLDRALFLRAHNFGMLCATFPVIKESIMLYNEQIVCGGKMGASDLYSLHAYIVQNVLPMDSNASSANTPVIKRSISECQAKGFVRCHPSDASEGQDEALPLTVYLSVENQVTPHYLLIYRALQITLCLFLDAKQPAPKQELYDELHAYIAPQLTCLARDIASEVSKEALAHPGQDSSSGGGPASNDTTPKYLFINEQSLQHHTNIHRNGIPRNVMSVIADLANPGERSNVAAAAATEELQVKTTNDYWIVKRCCNWRQYYVILCNSKATLLDVTQEAKRIFEQELTDDVFFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GP2</accession>
    <accession>A0A6I8UGA2</accession>
    <name>Q29GP2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Receptor-binding cancer antigen expressed on SiSo cells</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4815305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355011.2">
        <property type="nucleotide sequence ID" value="XM_001354975.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GP2"/>
    <dbReference type="SMR" id="Q29GP2"/>
    <dbReference type="FunCoup" id="Q29GP2">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GP2"/>
    <dbReference type="GeneID" id="4815305"/>
    <dbReference type="KEGG" id="dpo:4815305"/>
    <dbReference type="eggNOG" id="ENOG502SFPA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096308_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GP2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244516">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017025">
        <property type="entry name" value="Cancer-assoc_antigen_RCAS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15208:SF2">
        <property type="entry name" value="RECEPTOR-BINDING CANCER ANTIGEN EXPRESSED ON SISO CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15208">
        <property type="entry name" value="RECEPTOR-BINDING CANCER ANTIGEN EXPRESSED ON SISO CELLS CANCER ASSOCIATED SURFACE ANTIGEN RCAS1 ESTROGEN RECEPTOR-BINDING FRAGMENT- ASSOCIATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF034247">
        <property type="entry name" value="RCAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043994115" evidence="2">
        <location>
            <begin position="22"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355011.2"/>
        </source>
    </evidence>
    <sequence length="218" mass="24340" checksum="249380121ED9E06A" modified="2016-01-20" version="2">MLQQIRMLLLSIVTLCRRALCCFSRRRKLSHSGGGITSNGDQLQAVNVIVERGDFAAAAGSAGGGGGSRSIGRERDWNSWDDSPRTVEEHIEQYRQRMAQPPTPPKEEPEPDFFSELTPTIKPQVKYFLDDPTATAASNQQTDFSRLQAQDVVPISDTADLEDWVDDNPGGWEELDTSGTKQIIREKRREARQQRQPLTKAVQPTIGAQRLSDIHRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GP6</accession>
    <accession>A0A6I8UGF8</accession>
    <name>Q29GP6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein out</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">out</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355006.1">
        <property type="nucleotide sequence ID" value="XM_001354970.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GP6"/>
    <dbReference type="GeneID" id="4814951"/>
    <dbReference type="KEGG" id="dpo:4814951"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_2_1"/>
    <dbReference type="InParanoid" id="Q29GP6"/>
    <dbReference type="OMA" id="IGMSIMM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080787">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29GP6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000383">
        <property type="entry name" value="Blast:Monocarboxylate transporter 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF8">
        <property type="entry name" value="BCDNA.LD28120-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="548"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="583"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="457"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001355006.1"/>
        </source>
    </evidence>
    <sequence length="648" mass="72415" checksum="ABCF6A7323786FB8" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IL1</accession>
    <accession>A0A6I8UHG7</accession>
    <name>Q29IL1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4815848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355583.2">
        <property type="nucleotide sequence ID" value="XM_001355547.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IL1"/>
    <dbReference type="STRING" id="46245.Q29IL1"/>
    <dbReference type="GeneID" id="4815848"/>
    <dbReference type="KEGG" id="dpo:4815848"/>
    <dbReference type="eggNOG" id="ENOG502RZD1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010718_0_2_1"/>
    <dbReference type="InParanoid" id="Q29IL1"/>
    <dbReference type="OMA" id="CFYTTRE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072275">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF56">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="141"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355583.2"/>
        </source>
    </evidence>
    <sequence length="425" mass="49100" checksum="54D5D3FD8C0502CD" modified="2008-10-14" version="2">MKLESEKEKDKERRTGDPPKYLNEEFFKAALEDGLRDMRVDIKKIIFAESSGGGGENYCSKIYRARALYRSSKRQLDEELAMIVKSIAITPATQFLEELAVYLREKIFYFDVLGKLEVLIGDGSKFGAKCLYTTREPIQTIVFDDLTQYGYKLASRQSGLNEEHCVVILKKLGKFHASSMLLAQKEPGVGEHFTSGMLDENYIRTNERFINFMALQLRTLANLVAEWPGYEQLADKLHRHCDNIKENLVTTGRSLPGEITVLNHGDLWVNNFMYKYDDEQPTKPIDAIFVDFQNSFFGSPGCDINFFLNSSVQLDVLIHRREFLIQTYYGALRESLERMHFEFVPSYADIQQEIRARELYGFFSSYAFLPMVTMKKEDSYDISIEALSDPDFAKTKVQLMFSSNPRTTDTLRYALRRFDELGIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IM4</accession>
    <accession>A0A6I8VH49</accession>
    <name>Q29IM4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Synaptic vesicle glycoprotein 2B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC4815696</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355570.1">
        <property type="nucleotide sequence ID" value="XM_001355534.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041251.1">
        <property type="nucleotide sequence ID" value="XM_015185765.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IM4"/>
    <dbReference type="STRING" id="46245.Q29IM4"/>
    <dbReference type="GeneID" id="4815696"/>
    <dbReference type="KEGG" id="dpo:4815696"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_15_1"/>
    <dbReference type="OMA" id="RFQIEFH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076404">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IM4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000232">
        <property type="entry name" value="Organic cation/carnitine transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF34">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="488"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="601"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="148"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001355570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015041251.1"/>
        </source>
    </evidence>
    <sequence length="629" mass="68590" checksum="B4C98931C06BC9C9" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q29K41</accession>
    <accession>A0A6I8UJA6</accession>
    <name>Q29K41_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleoporin NUP145 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fon</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356274.1">
        <property type="nucleotide sequence ID" value="XM_001356238.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29K41"/>
    <dbReference type="FunCoup" id="Q29K41">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29K41"/>
    <dbReference type="GeneID" id="4816616"/>
    <dbReference type="KEGG" id="dpo:4816616"/>
    <dbReference type="eggNOG" id="KOG0845">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502758_0_0_1"/>
    <dbReference type="InParanoid" id="Q29K41"/>
    <dbReference type="OMA" id="PTVQYVQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074007">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044270172" evidence="2">
        <location>
            <begin position="17"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356274.1"/>
        </source>
    </evidence>
    <sequence length="566" mass="57057" checksum="1BB4793838F16D7B" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LN8</accession>
    <accession>A0A6I8UKG7</accession>
    <name>Q29LN8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Der-1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356941.1">
        <property type="nucleotide sequence ID" value="XM_001356905.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LN8"/>
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        <property type="interactions" value="1150"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LN8"/>
    <dbReference type="GeneID" id="4817526"/>
    <dbReference type="KEGG" id="dpo:4817526"/>
    <dbReference type="eggNOG" id="KOG0858">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051898_3_1_1"/>
    <dbReference type="InParanoid" id="Q29LN8"/>
    <dbReference type="OMA" id="LWRCVTS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070687">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LN8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356941.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="28059" checksum="5A663164EEDB647F" modified="2006-04-04" version="1">MDIGQWYRSLPRFTRYWLTTTVLLSLLCRFELLDIWSLSLNRSMVFGKLQLWRCVTSLFVFPITPNTAFHFLINCYFIVQYSSRLEKDQYGRSPSDYLYLLIVTSVLANIGGMLFNVQFLMDMMVMAITYVWCQLNKDVTVSFWFGSRFKAMYLPWVLAAFEFIFHFSLASLIGIFNGHVYFFLKFQYSQELGGNALLETPQFLKRLVPDTSGGFGGFGVPPESRAPPPPESPWGRGMALGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MR2</accession>
    <accession>A0A6I8UJQ0</accession>
    <name>Q29MR2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Radial spoke head protein 9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Rsph9</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Kinocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the flagellar radial spoke RSP9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356568.1">
        <property type="nucleotide sequence ID" value="XM_001356532.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MR2"/>
    <dbReference type="FunCoup" id="Q29MR2">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MR2"/>
    <dbReference type="GeneID" id="4817113"/>
    <dbReference type="KEGG" id="dpo:4817113"/>
    <dbReference type="eggNOG" id="ENOG502QR99">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068343_1_0_1"/>
    <dbReference type="InParanoid" id="Q29MR2"/>
    <dbReference type="OMA" id="TFYHVPN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076504">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MR2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060091">
        <property type="term" value="C:kinocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055316">
        <property type="entry name" value="RSP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356568.1"/>
        </source>
    </evidence>
    <sequence length="281" mass="32704" checksum="FBC5DCC2AC395058" modified="2006-04-04" version="1">MNLEYFSEGMDCLMYCGLKLSPEQRILIENSLIALQNDNRFSGMYLWGRITATKQDYYIAFGYTNDCLKDRKYFYSLDQFQWQLLPFVQSPKIFQATTLARESFVGDPSLMTYVKLDPTFEIVGNHVVAISRPEVVKLKEEERLAAIVFIITEECAVCPRGALYKMTDGRVIPNQMFRGLDNLQVDNQSFYQLYRLPRNDLKVNLSKRSDYNYPIDFLDTIDCVIPLGQAFALNLQRNERLVVIKSCLWLGMTFFHKITSHKHGFLYLGDGKKNSDLLFMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NM5</accession>
    <accession>A0A6I8UKU5</accession>
    <name>Q29NM5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein cornichon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cni</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357127.2">
        <property type="nucleotide sequence ID" value="XM_001357091.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NM5"/>
    <dbReference type="FunCoup" id="Q29NM5">
        <property type="interactions" value="1402"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NM5"/>
    <dbReference type="GeneID" id="4817758"/>
    <dbReference type="KEGG" id="dpo:4817758"/>
    <dbReference type="eggNOG" id="KOG2729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112942_1_0_1"/>
    <dbReference type="InParanoid" id="Q29NM5"/>
    <dbReference type="OMA" id="FAVFHVI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079179">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033466">
        <property type="entry name" value="Cornichon_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01340">
        <property type="entry name" value="CORNICHON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001357127.2"/>
        </source>
    </evidence>
    <sequence length="144" mass="16790" checksum="DE199A3F676D5D53" modified="2008-10-14" version="2">MVFNFTAFTYIVALIGDAFLIFFAIFHVIAFDELKTDYKNPIDQCNSLNPLVLPEYLLHIFLNLLFLVCGEWFSLAINIPLIAYHIWRYKTRPVMSGPGLYDPTTVLKTDTLSRNMREGWIKLAVYLISFFYYIYGMVYSLIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NS7</accession>
    <accession>A0A6I8ULP5</accession>
    <name>Q29NS7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phospholipase D3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Pld3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357497.2">
        <property type="nucleotide sequence ID" value="XM_001357461.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NS7"/>
    <dbReference type="FunCoup" id="Q29NS7">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NS7"/>
    <dbReference type="GeneID" id="4818185"/>
    <dbReference type="KEGG" id="dpo:4818185"/>
    <dbReference type="eggNOG" id="KOG3603">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027021_3_1_1"/>
    <dbReference type="InParanoid" id="Q29NS7"/>
    <dbReference type="OMA" id="WTHFIPN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081628">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09106">
        <property type="entry name" value="PLDc_vPLD3_4_5_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09107">
        <property type="entry name" value="PLDc_vPLD3_4_5_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050874">
        <property type="entry name" value="Diverse_PLD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032803">
        <property type="entry name" value="PLDc_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185:SF17">
        <property type="entry name" value="GM01519P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185">
        <property type="entry name" value="PHOSPHOLIPASE D - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13918">
        <property type="entry name" value="PLDc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="421"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357497.2"/>
        </source>
    </evidence>
    <sequence length="491" mass="55459" checksum="418C1A5E75AA3CB4" modified="2008-10-14" version="2">MPQQYKPLHNEEDVENAAATAAVTTGDNRQQQQEAANPPAVQLLMVSLFMLLFFGSSFFQPRPRLQHLKGSGNKYDSTKYGCNIQLVESIPIGLNYSEGSPRFLSTFEAWQQLLDSAKESLDIASFYWTMRAQDTPGVNDSSTQPGDEIFRRFLANGNGGGKTPRLNIRIAQSEPSSVSPNEDTKLLASNGAAEVVTLSFPKYFGGGVLHTKLWIVDEQHFYLGSANMDWRALTQVKEMGILAQNCPQLTRDVALIFKAYWCLGKSTDAKIPYWGPDYFTKYNLQSPMILNVNQNTTMEGFLSSSPPPLSAQSRTGDLEAILSTIDSAITYVNIAVMDYYPLIIYGQHPKYWPYIDDALRRAAVERGVSVKLLISWWKHSNPSENKYLKSLQDLSSKKDKIDIQIRRFIVPTDADQEKIPYGRVNHNKYMVTDRVAYIGTSNWSGDYFTDTAGIGLVLRETHETEHTQTLRSDLRNVFERDWNSPYAHELE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NZ8</accession>
    <name>FUZZY_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Protein fuzzy homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fuz</name>
        <name type="synonym">fuzzy</name>
        <name type="synonym">fy</name>
        <name type="ORF">GA12254</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the organization of cell polarity via a planar cell polarity (PCP) cascade. Involved in the development of the hairs on the wings. May specify the correct orientation of the hair by limiting the site of prehair initiation to the distal vertex of the wing cells. May control wing cell hair number by maintaining the integrity of the cytoskeletal components that direct hair development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fuzzy family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379058">
        <property type="protein sequence ID" value="EAL34495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NZ8"/>
    <dbReference type="SMR" id="Q29NZ8"/>
    <dbReference type="FunCoup" id="Q29NZ8">
        <property type="interactions" value="569"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0281002">
        <property type="protein sequence ID" value="FBpp0279440"/>
        <property type="gene ID" value="FBgn0072300"/>
    </dbReference>
    <dbReference type="GeneID" id="4817938"/>
    <dbReference type="KEGG" id="dpo:4817938"/>
    <dbReference type="CTD" id="34166"/>
    <dbReference type="eggNOG" id="ENOG502QVMY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041212_1_0_1"/>
    <dbReference type="InParanoid" id="Q29NZ8"/>
    <dbReference type="OMA" id="LDQYSCS"/>
    <dbReference type="PhylomeDB" id="Q29NZ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072300">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026069">
        <property type="entry name" value="Fuzzy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13559">
        <property type="entry name" value="INTRACELLULAR TRAFFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13559:SF1">
        <property type="entry name" value="PROTEIN FUZZY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0206">Cytoskeleton</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000312925" description="Protein fuzzy homolog">
        <location>
            <begin position="1"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="416" mass="46215" checksum="6E6C18630A26F38C" modified="2006-04-04" version="1">MSIYLLCLTTNGGLPVFTRKKGECDNLPFSTVASLNGFHMFFKSLGIQLEATTTSKQPLQAQGSGLADPEEWTYIWRDRNAVTLIVCSSGVGSEQLLQSLADLVFGAIGMFITRASEMAHATLLERLKKDAKKYVPIVDAILEAACAGTQLLGYTDCVLTSDNGQLLQCLNEFSGHCGSLFCCLVVGHRIAVATEGWWDLDTIDRELLLFLLNSSSTLQHDVPVYLPVKSPNIAYRFVSIPVAPNSALCVLCGAENASFRELHMHAMNAYRNEGNVLAAAERCVPRSLPEHLDIDGNVLAIILINRQSRKSLFTRNLHQSASAKRIIVGDLQRLDMLKKFFDQTMETIQQFEANRETTTVLMDQYSCSDYHKCYAYADEQSNVIFLLFVAAVPTHAMRFLAQRVHASILQEKSVCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ71</accession>
    <accession>A0A6I8UDP5</accession>
    <name>Q2LZ71_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA-directed RNA polymerase III subunit RPC8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA-directed RNA polymerase III subunit H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">DNA-directed RNA polymerase III subunit rpc8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Polr3H</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. With CRCP/RPC9 forms a mobile stalk that protrudes from Pol III core and functions primarily in transcription initiation. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the RNA polymerase III complex consisting of 17 subunits: a ten-subunit horseshoe-shaped catalytic core composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and functioning primarily in transcription initiation; and additional subunits homologous to general transcription factors of the RNA polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer required for transcription initiation and POLR3D/RPC4-POLR3E/RPC5 heterodimer involved in both transcription initiation and termination. Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353902.1">
        <property type="nucleotide sequence ID" value="XM_001353866.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ71"/>
    <dbReference type="FunCoup" id="Q2LZ71">
        <property type="interactions" value="1360"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZ71"/>
    <dbReference type="GeneID" id="4813619"/>
    <dbReference type="KEGG" id="dpo:4813619"/>
    <dbReference type="eggNOG" id="KOG3297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073901_1_1_1"/>
    <dbReference type="InParanoid" id="Q2LZ71"/>
    <dbReference type="OMA" id="LGPTLWW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080270">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZ71">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04330">
        <property type="entry name" value="RNAP_III_Rpc25_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000130">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3 18" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="15">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III subunit Rpc25" evidence="16">
        <location>
            <begin position="84"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="211"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001353902.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="26519" checksum="2FE63694611A2986" modified="2006-02-21" version="1">MFVLAELKDTVRIAPDQFSVKLVDAIRDEIDRKLANKVLLNVGLCIALKDIVSLQDSIILPGDGASHTAVVFRYIVFRPKVGTVVTGKIRSCSREGVHVTLGFFDDILIPHAALQHPSRFDEAEQAWVWEYPLEDGAKHDLFMDVGEPIKFRVSREIFEETSPVGPPKSESQSTQGPSTSTAAATAAQEIKTPYKIIGAINESGLGVLSWWDQQGKEDDQENEDNTEYDNDEDGEGACEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M038</accession>
    <accession>A0A6I8UD08</accession>
    <name>Q2M038_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RpS4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353585.1">
        <property type="nucleotide sequence ID" value="XM_001353549.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M038"/>
    <dbReference type="SMR" id="Q2M038"/>
    <dbReference type="FunCoup" id="Q2M038">
        <property type="interactions" value="1107"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M038"/>
    <dbReference type="GeneID" id="4813070"/>
    <dbReference type="KEGG" id="dpo:4813070"/>
    <dbReference type="eggNOG" id="KOG0378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060400_1_0_1"/>
    <dbReference type="InParanoid" id="Q2M038"/>
    <dbReference type="OMA" id="GHIQLNL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070939">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000051">
        <property type="entry name" value="40S ribosomal protein S4, X isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="8">
        <location>
            <begin position="43"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353585.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29122" checksum="6E373AAE8E2714B6" modified="2006-02-21" version="1">MARGPKKHLKRLAAPKAWMLDKLGGAFAPRPSTGPHKLRESLPLLIFLRNRLKYALNGAEVTKIVMQRLVKVDGKVRTDPTYPAGFMDVITLEKTGEFFRLVYDVKGRFTIHRISAEEAKYKLCKVKKNQLGAKGVPFLVTHDGRTIRYPDPLIHANDTVQVDIATGKITDYIKFDSGNLCMITGGRNLGRVGTVVNRERHPGSFDIVHIKDSQGHVFATRLTNVFIIGKGSKPYISLPKGKGVKLSISEERDKRLAAKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NXL5</accession>
    <accession>A0A6I8VHC9</accession>
    <name>A0A0R3NXL5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Cdc7</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_015040893.1">
        <property type="nucleotide sequence ID" value="XM_015185407.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NXL5"/>
    <dbReference type="GeneID" id="4815978"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077125">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015040893.1"/>
        </source>
    </evidence>
    <sequence length="510" mass="57265" checksum="E1BA5AB07CDADA34" modified="2016-01-20" version="1">MDNVVGIHCCLRCDASAAFVMPYMPHDRFHDFYTKMDVSEIRLYMRNLLVALRHVHKFNVIHRDVKPSNFLYNRRRREFLLVDFGLAQHVSPPVPAPGTAVGQQEQRSRHTGGAGGPHSKRFRDRETEALQTTVAAATAVAAADAGLGGAVKRMRLNEEGGWSKMPLKPVNEIAHAQNELKEVVPQVPETQLSTQNIHQQQQHMQPDQTTSTGTGTASTCSKYNTNRSATGSTNSAKCFCFANPAVCLNCLMKKEVHASRAGTPGYRPPEVLLKYADQTTAVDVWSVGVIFLSLMSSVYPFFKAPNDYIALAEIVTIFGDQEIRKTALALERMVTLSQRSRPLNLRKLCLRFRNRAIFSDAKMLKKYEAPDGQCEVCKNCDQYFFNCLCEESDYVTEPLEPYEVFPASAYDLLERLLEVNPHKRITAAEALQHSFFNCQGQGQCTQSGGDEQVANTPRKGRTASTRQMTRRRFANRAVSMGIQPLQQLEIEQQLLYPAKLEELQTVLNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NYB1</accession>
    <accession>A0A6I8VHD0</accession>
    <name>A0A0R3NYB1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Double-stranded RNA-specific editase Adar isoform X6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">Adar</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041122.1">
        <property type="nucleotide sequence ID" value="XM_015185636.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041132.1">
        <property type="nucleotide sequence ID" value="XM_015185646.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NYB1"/>
    <dbReference type="SMR" id="A0A0R3NYB1"/>
    <dbReference type="GeneID" id="4815776"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071761">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NYB1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003726">
        <property type="term" value="F:double-stranded RNA adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006382">
        <property type="term" value="P:adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19865">
        <property type="entry name" value="DSRM_STRBP_RED-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000044">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000049">
        <property type="entry name" value="Uncharacterized protein, isoform I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910:SF62">
        <property type="entry name" value="AT07585P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="55"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="222"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="A to I editase" evidence="4">
        <location>
            <begin position="355"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015041122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015041132.1"/>
        </source>
    </evidence>
    <sequence length="683" mass="76098" checksum="EB8DE7EC2A7902CE" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P8C0</accession>
    <accession>A0A6I8VK17</accession>
    <name>A0A0R3P8C0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein cycle isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cyc</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042973.1">
        <property type="nucleotide sequence ID" value="XM_015187487.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P8C0"/>
    <dbReference type="SMR" id="A0A0R3P8C0"/>
    <dbReference type="FunCoup" id="A0A0R3P8C0">
        <property type="interactions" value="726"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P8C0"/>
    <dbReference type="GeneID" id="4813542"/>
    <dbReference type="eggNOG" id="KOG3561">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3P8C0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081272">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P8C0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
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        <property type="term" value="F:protein dimerization activity"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
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        <property type="entry name" value="PAS domain"/>
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        <property type="entry name" value="bHLH_dom"/>
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        <property type="entry name" value="Circadian_TF"/>
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        <property type="entry name" value="HLH_DNA-bd_sf"/>
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        <property type="entry name" value="Nuc_translocat"/>
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        <property type="entry name" value="PAS"/>
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        <property type="entry name" value="NCTRNSLOCATR"/>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
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        <location>
            <begin position="242"/>
            <end position="261"/>
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            <begin position="12"/>
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            <begin position="31"/>
            <end position="41"/>
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            <begin position="42"/>
            <end position="63"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015042973.1"/>
        </source>
    </evidence>
    <sequence length="434" mass="49413" checksum="8A0F64BD46ADECC7" modified="2016-01-20" version="1">MLSLKSPEETQTDALASTVSMMSSAVNSSENQEDLEEDFDDEKPANRTSDENRKQNHSEIEKRRRDKMNKCINELSSMIPMCYAMQRKLDKLTVLRMAVQHLRGIRGSGSLHPYVGADFRPSFMSDQELKMIILQASEGFLFVVGCDRGRILYVSESVSSVLDCTQADLLGQSWFDVLHPKDIGKVKEQLSSLEQCPRERLIDAKTMLPVKTDVPQSLCRLCPGARRSFFCRMKLRAANNQIKEESDTSSSSRSSTKRKSKLSIDHKYRVIQCTGYLKSWTPIKDEEQEGDSDDQTTNLSCLVAIGRIPPNVLNSCVPTAVENHPNIQHVLFISRHSAEGKFLFIDQRATLVIGFLPQEILGTSFYEYFHNEDIAALVESHKIVMQVPGKLTTQVYRFRCKDSSYIQLQSEWRAFKNPWTNEIDYIIAKNSVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAN9</accession>
    <name>A0A6I8UAN9_DROPS</name>
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        <recommendedName>
            <fullName evidence="6">5'-nucleotidase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Nt5a</name>
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    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
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        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
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        <property type="nucleotide sequence ID" value="XM_001352419.4"/>
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        <property type="component" value="Chromosome X"/>
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    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="LD24878p"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
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    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
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    <dbReference type="NCBIfam" id="TIGR02244">
        <property type="entry name" value="HAD-IG-Ncltidse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF38">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="5_nucleotid"/>
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        <property type="entry name" value="HAD-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352455.4"/>
        </source>
    </evidence>
    <sequence length="551" mass="62275" checksum="E5F2E540D86F876C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAU0</accession>
    <name>A0A6I8UAU0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phenoloxidase-activating factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Prophenoloxidase-activating factor II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4811979</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352563.3">
        <property type="nucleotide sequence ID" value="XM_001352527.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAU0"/>
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        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811979"/>
    <dbReference type="InParanoid" id="A0A6I8UAU0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF142">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026238352" description="Phenoloxidase-activating factor 2" evidence="7">
        <location>
            <begin position="20"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="956"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="142"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="540"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="774"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="862"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="797"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="883"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00076468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001352563.3"/>
        </source>
    </evidence>
    <sequence length="1203" mass="129888" checksum="1263EFE9E7E0544A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB78</accession>
    <name>A0A6I8UB78_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein Asciz</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Asciz</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352743.4">
        <property type="nucleotide sequence ID" value="XM_001352707.4"/>
    </dbReference>
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        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812441"/>
    <dbReference type="InParanoid" id="A0A6I8UB78"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055303">
        <property type="entry name" value="ATMIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46664">
        <property type="entry name" value="ATM INTERACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46664:SF1">
        <property type="entry name" value="ATM INTERACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352743.4"/>
        </source>
    </evidence>
    <sequence length="476" mass="54852" checksum="4D7BDCBFBE2FC1C6" modified="2020-08-12" version="1">MECSKDINNIKYTPNISELLPEREYRCEKCGDQVFSNHSHYQLHLRQRHKVVISKQNFSVFHCPVERCRYHASAMARVEGRSFSSLRLVRQHFQKIHLAKNHKCPHCSEKFLLQRDLDRHKCLVHICNVCGLTYSSAAGLRTHMRRKNHLLDKNDGKVAIPALRTWQKLQEITVTTNENLPTIPAMPLTPESSTEAAEPEQTYAWYLPPIDVQCVLQLPQDSQREKLDMETQTEFEDPNEILVPPLRNIETQTPDDNWDQGTIGEMTMVTATVVQETQTPEDNWYHGTLAEMTTELNSELPVQAHTAATSVGHIETQTPDDNWDQETMTMVVQETQTPDDNWDPETMIEMTQVQEQTAATSVGHIETQTPAENWDQVQQHTASTSVDDGFPSYPDNEPMFGAQTSANMYTQTCDDLFEELGLSHIQTQTHWPDDGLYNTQHTQTCDEMLDELLENFTSTYTQTRWLDWQDNEDNDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBL0</accession>
    <name>A0A6I8UBL0_DROPS</name>
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        <submittedName>
            <fullName evidence="8">SET domain-containing protein SmydA-8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SmydA-2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352921.3">
        <property type="nucleotide sequence ID" value="XM_001352885.4"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053010">
        <property type="entry name" value="SET_SmydA-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455:SF1">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 4, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="5">
        <location>
            <begin position="45"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352921.3"/>
        </source>
    </evidence>
    <sequence length="532" mass="60517" checksum="7FF6FF38EE3B2CEF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UC36</accession>
    <name>A0A6I8UC36_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NF-X1-type zinc finger protein NFXL1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4812669</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFX1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353053.3">
        <property type="nucleotide sequence ID" value="XM_001353017.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UC36"/>
    <dbReference type="FunCoup" id="A0A6I8UC36">
        <property type="interactions" value="2590"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812669"/>
    <dbReference type="InParanoid" id="A0A6I8UC36"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UC36">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd16697">
        <property type="entry name" value="RING-CH-C4HC3_NFXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF1">
        <property type="entry name" value="NF-X1-TYPE ZINC FINGER PROTEIN NFXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="823"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="252"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="524"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="599"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="638"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="8">
        <location>
            <begin position="706"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="764"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353053.3"/>
        </source>
    </evidence>
    <sequence length="844" mass="94241" checksum="834363191A097F4C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCE6</accession>
    <name>A0A6I8UCE6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 70 homolog, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4813575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353256.2">
        <property type="nucleotide sequence ID" value="XM_001353220.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCE6"/>
    <dbReference type="FunCoup" id="A0A6I8UCE6">
        <property type="interactions" value="1102"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813575"/>
    <dbReference type="InParanoid" id="A0A6I8UCE6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCE6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009724">
        <property type="entry name" value="TMEM70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045325">
        <property type="entry name" value="TMEM70/TMEM186/TMEM223"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13281">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 70, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13281:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 70, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06979">
        <property type="entry name" value="TMEM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353256.2"/>
        </source>
    </evidence>
    <sequence length="238" mass="26531" checksum="FDC17AAB9AE66199" modified="2020-08-12" version="1">MFSLRAALPRGKNFAVAFGNAARHIPLAPSCRFGGSAWTSVPLQQEKQYKQQNLQCRRLADQAAGKGAETELQRVYYGTLAPRMKLVKFFSLSTSLAGIAAQPILLEQGMKIGGTGMAVFLCTIGGFFTFVTPLLLHFITKKYVTELHYNPATEEYTATTISLLLQKIKTKFRPGDVTVPEVPGMFTSFLVNKRPLFIDPALFEDPEHYVRIMGYDKPIDFKLDQSPGSEKQTKSQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF77</accession>
    <name>A0A6I8UF77_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleosome assembly protein 1-like 1-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4814409</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354490.4">
        <property type="nucleotide sequence ID" value="XM_001354454.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UF77"/>
    <dbReference type="FunCoup" id="A0A6I8UF77">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814409"/>
    <dbReference type="InParanoid" id="A0A6I8UF77"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UF77">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="315"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354490.4"/>
        </source>
    </evidence>
    <sequence length="350" mass="40312" checksum="A11E13C3B6AF2F86" modified="2020-08-12" version="1">MYAIERDPELEQQQDKGSESLEEGASSATMRHDEKSSESLDRCCSFDSMLCPADMSARSRKAYLQHMMDELPRPVHNRILAMKHSQMEQIKISEQFYREVYELEKRFYLQCAELFDSRRDIVDGSLEPEPMEPSWSEQHDDLVAELQASKEFHQLTELMPKMPANAVGVPRFWLSIFRNVSLLSDMVQHHDEPLLECLMDVRISYEPESYTLKFLFRPNSYLDDCSLMLTKKYLLRHQADQEYPFMFEGPEIVSCEGCHIHWRDDSNLTMQTDLSLADEKTKMLLGNDFEVGFLLRTQIVPKAVLFYTGDLVDSTNEGLSDSDSSETSDTDADTDTTAPAPTHTPEQLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHA8</accession>
    <name>A0A6I8UHA8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">27 kDa hemolymph protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4816077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355470.4">
        <property type="nucleotide sequence ID" value="XM_001355434.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHA8"/>
    <dbReference type="KEGG" id="dpo:4816077"/>
    <dbReference type="InParanoid" id="A0A6I8UHA8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHA8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009832">
        <property type="entry name" value="DUF1397"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20997">
        <property type="entry name" value="EG:BACR42I17.2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20997:SF2">
        <property type="entry name" value="EG:BACR42I17.2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07165">
        <property type="entry name" value="DUF1397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026162317" evidence="1">
        <location>
            <begin position="25"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355470.4"/>
        </source>
    </evidence>
    <sequence length="319" mass="34727" checksum="51934B0B6AEFABC8" modified="2020-08-12" version="1">MNQYALMAVCLMAALGAILLEVQANPSPAVATPQLSPSQLGGLSAQFLPPEYRNTNISVEDLKRMYREKCKKVTGAENATFYQEIEKAAAKMSACISGVANLTALQAEMEEAKPLGELDTVFHKYCLRAPEAEACVKEFNEKMQVCLTSEEKRQQETIARIGASLLGFACSRGGDQIALFVAEQGPECLEANKDAISNCLNRSFHQYIPKDGQVADLMSRPELLFSPTHCVDLQSFEACVLHHLEQCSEITPANVVQSIFRFVKNETDCQAYMEARANERPVLLAARSNSTGGGASDLSTSTTLLGSLVLGLTSLLIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIM6</accession>
    <name>A0A6I8UIM6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Outer dense fiber protein 3-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4816408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356068.4">
        <property type="nucleotide sequence ID" value="XM_001356032.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIM6"/>
    <dbReference type="KEGG" id="dpo:4816408"/>
    <dbReference type="InParanoid" id="A0A6I8UIM6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIM6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356068.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9129" checksum="20E090DDFD045421" modified="2020-08-12" version="1">MSSNFGPGPAAYMLPSQFDKGAGRGPQYSFGRRTGKKTIDVVGPGPAAYKVDNVTRHGRSDGLQFSILRRRPIKPAKIRYDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJN5</accession>
    <name>A0A6I8UJN5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">wol</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001356601.3">
        <property type="nucleotide sequence ID" value="XM_001356565.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJN5"/>
    <dbReference type="FunCoup" id="A0A6I8UJN5">
        <property type="interactions" value="1959"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817412"/>
    <dbReference type="InParanoid" id="A0A6I8UJN5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJN5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000068">
        <property type="entry name" value="ALG5, dolichyl-phosphate beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="15">
        <location>
            <begin position="69"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001356601.3"/>
        </source>
    </evidence>
    <sequence length="326" mass="36654" checksum="4495A69313D9A8D8" modified="2020-08-12" version="1">MCPCLLQLLFYLLATAVATGFVIVAVVLYKTKPYPNIVRHSDEKSFLDPQSIQTVAFPSIEDAPTLELSVIVPAYNEEKRLPSMLDECLAFLEEKSKGKSSFNYEVIVVSDGSADATVSVALRYAKKHGADKVRVMELVENRGKGGAVRMGVLSARGRQLLFADADGATKFADYDKLEESLSSLASDWRHDGIAIGSRAHLEDDAIASRSFFRTILMHGFHTLVWLFAVRSVRDTQCGFKLFTRSTARKLFTSLHVQRWAFDVELLYLAERLKLPMSEVAVRWTEIDGSKLTPFWSWLQMGSDLGMIWLRYTVGAWRIAAIEKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL79</accession>
    <name>A0A6I8UL79_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase assembly factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357223.4">
        <property type="nucleotide sequence ID" value="XM_001357187.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL79"/>
    <dbReference type="FunCoup" id="A0A6I8UL79">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817922"/>
    <dbReference type="InParanoid" id="A0A6I8UL79"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014807">
        <property type="entry name" value="Coa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47148">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47148:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08695">
        <property type="entry name" value="Coa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357223.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="26871" checksum="E449538FDB0A4751" modified="2020-08-12" version="1">MLLALLAKFNTMPTLKMRLTLPSRRSLANLCVFGAMASLSGLAYLHWKLEDRVRQADYYQLAIKTLRQHGGAVRLLGEPIKETGFSLTNNRNTCDENRAQLEVAVHGTKDKGTVFFWATNNREKGWLIDRLELETKQYPNTRFLLKKPTGRALQDVGNEEDDEEHEHAHIVVPEPKPLGPQMPQHPEQPSQQEPHAFDQPGYQEGPVQGGPNQPKTLHQHQGQEPAPYIQTADGGRMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULB0</accession>
    <name>A0A6I8ULB0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein 436</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">prg</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357265.4">
        <property type="nucleotide sequence ID" value="XM_001357229.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULB0"/>
    <dbReference type="FunCoup" id="A0A6I8ULB0">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817998"/>
    <dbReference type="InParanoid" id="A0A6I8ULB0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001228">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF53">
        <property type="entry name" value="CHORION TRANSCRIPTION FACTOR CF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="15">
        <location>
            <begin position="19"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="250"/>
            <end position="278"/>
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        <location>
            <begin position="279"/>
            <end position="306"/>
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        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
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        <location>
            <begin position="335"/>
            <end position="362"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="435"/>
            <end position="457"/>
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        <location>
            <begin position="462"/>
            <end position="485"/>
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        <location>
            <begin position="489"/>
            <end position="516"/>
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    </feature>
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        <location>
            <begin position="140"/>
            <end position="183"/>
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        <location>
            <begin position="142"/>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="21"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="24"/>
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        <ligand>
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        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="77"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="PROSITE" id="PS51915"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001357265.4"/>
        </source>
    </evidence>
    <sequence length="575" mass="65864" checksum="CA3F02C95067FE64" modified="2020-08-12" version="1"> </sequence>
</entry>
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        <submittedName>
            <fullName evidence="4">Protein mushroom body miniature</fullName>
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        <name evidence="4" type="primary">mbm</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
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        <property type="term" value="F:zinc ion binding"/>
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            <dbReference type="RefSeq" id="XP_001357461.4"/>
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</entry>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Neoptera</taxon>
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        <citation type="submission" date="2022-04" db="UniProtKB">
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                <consortium name="RefSeq"/>
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            <strain evidence="16 17">MV-25-SWS-2005</strain>
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        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
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        <text evidence="4">Belongs to the MIEAP family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <location>
            <begin position="32"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="41"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00008233"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032687"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16026"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001357692.3"/>
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    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033241841.1"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMC3</accession>
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        <recommendedName>
            <fullName evidence="7">GATOR2 complex protein WDR24</fullName>
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        <name evidence="11" type="primary">Wdr24</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR24 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357776.4">
        <property type="nucleotide sequence ID" value="XM_001357739.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMC3"/>
    <dbReference type="SMR" id="A0A6I8UMC3"/>
    <dbReference type="FunCoup" id="A0A6I8UMC3">
        <property type="interactions" value="1530"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800511"/>
    <dbReference type="InParanoid" id="A0A6I8UMC3"/>
    <dbReference type="OMA" id="EPMWLIS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080473">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16693">
        <property type="entry name" value="mRING-H2-C3H3C2_WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="101"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="461"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="465"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357776.4"/>
        </source>
    </evidence>
    <sequence length="772" mass="86862" checksum="9B801612C9614955" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN51</accession>
    <name>A0A6I8UN51_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CDK5RAP1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4801020</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Potential regulator of CDK5 activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358190.3">
        <property type="nucleotide sequence ID" value="XM_001358153.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN51"/>
    <dbReference type="FunCoup" id="A0A6I8UN51">
        <property type="interactions" value="1824"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801020"/>
    <dbReference type="InParanoid" id="A0A6I8UN51"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035597">
        <property type="term" value="F:tRNA-2-methylthio-N(6)-dimethylallyladenosine(37) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01574">
        <property type="entry name" value="miaB-methiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020:SF2">
        <property type="entry name" value="MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00413">
        <property type="entry name" value="CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="11">
        <location>
            <begin position="98"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="243"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="10">
        <location>
            <begin position="497"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001358190.3"/>
        </source>
    </evidence>
    <sequence length="589" mass="65795" checksum="0669A727CE71EEDF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN64</accession>
    <name>A0A6I8UN64_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 23 member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4801033</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358201.4">
        <property type="nucleotide sequence ID" value="XM_001358164.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN64"/>
    <dbReference type="FunCoup" id="A0A6I8UN64">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801033"/>
    <dbReference type="InParanoid" id="A0A6I8UN64"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001358201.4"/>
        </source>
    </evidence>
    <sequence length="587" mass="63747" checksum="E8A0771B821CFCB0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN95</accession>
    <name>A0A6I8UN95_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Polr1H</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Can mediate Pol I proofreading of the nascent RNA transcript. Anchors into the Pol I active site to monitor transcription fidelity and cleave mis-incorporated 5'-ribonucleotides.</text>
    </comment>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358146.1">
        <property type="nucleotide sequence ID" value="XM_001358109.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN95"/>
    <dbReference type="SMR" id="A0A6I8UN95"/>
    <dbReference type="FunCoup" id="A0A6I8UN95">
        <property type="interactions" value="1472"/>
    </dbReference>
    <dbReference type="GeneID" id="4800964"/>
    <dbReference type="KEGG" id="dpo:4800964"/>
    <dbReference type="InParanoid" id="A0A6I8UN95"/>
    <dbReference type="OMA" id="EMQYHTL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072316">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10507">
        <property type="entry name" value="Zn-ribbon_RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000053">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034004">
        <property type="entry name" value="Zn_ribbon_RPA12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF14">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="78"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="11">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358146.1"/>
        </source>
    </evidence>
    <sequence length="121" mass="13632" checksum="9E978E47E352DE4C" modified="2020-08-12" version="1">METFNNCPGFCPSCGSILPPLQVKGNVICHNCHQEFLPDVYGGEKTEYSIQFNTYDPSKVFNRTKRESESSEADGPVVERKCPKCGHDKMSYATLQLRSADEGQTVFFTCLKCKYKESENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNW1</accession>
    <name>A0A6I8UNW1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-Q</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cyclin-related protein FAM58A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">koko</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin-like FAM58 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358541.5">
        <property type="nucleotide sequence ID" value="XM_001358504.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNW1"/>
    <dbReference type="FunCoup" id="A0A6I8UNW1">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801447"/>
    <dbReference type="InParanoid" id="A0A6I8UNW1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0262671">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20534">
        <property type="entry name" value="CYCLIN_CCNM_CCNQ_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20535">
        <property type="entry name" value="CYCLIN_CCNM_CCNQ_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000122">
        <property type="entry name" value="Cyclin-related protein FAM58A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000042">
        <property type="entry name" value="FAM58A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048055">
        <property type="entry name" value="Cyclin-Q_first_cyclin_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048053">
        <property type="entry name" value="Cyclin-Q_second_cyclin_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026113198" description="Cyclin-Q" evidence="5">
        <location>
            <begin position="18"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin N-terminal" evidence="6">
        <location>
            <begin position="72"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358541.5"/>
        </source>
    </evidence>
    <sequence length="303" mass="34196" checksum="BFDDC8B5A2AA93D3" modified="2020-08-12" version="1">MHFVALIIGSLSPTVGANELPRLHFGITVRATVLQSSICTANKMKNVLDVMSLQQHVESSKAQAQLKPLDYRKTNMPGIVPLYIFECATKLKMKPLTAACAAIVYHRFFKEVKPTDYDEFLIAASSLYLAGKIKDDDSVKIRDVINVAYCTLNRGNAPLDLNDEYWSMRDAIVQAELLITRTLGFDLNIDLAHKYLLYYMKTLQDWVGAEVWNSVPIAKAAASYLQDFHHSANILKYKPTHVAIGCLSLAMQTYGIQVPLTDEAEEGSMWYKPLVKDFTREQQWEIIENVIEVYKNEALLKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP57</accession>
    <name>A0A6I8UP57_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Beta-1,4-N-acetylgalactosaminyltransferase bre-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">beta4GalNAcTB</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358670.1">
        <property type="nucleotide sequence ID" value="XM_001358633.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP57"/>
    <dbReference type="FunCoup" id="A0A6I8UP57">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="GeneID" id="4801602"/>
    <dbReference type="KEGG" id="dpo:4801602"/>
    <dbReference type="InParanoid" id="A0A6I8UP57"/>
    <dbReference type="OMA" id="FASHYDY"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073086">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033842">
        <property type="term" value="F:N-acetyl-beta-glucosaminyl-derivative 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006688">
        <property type="term" value="P:glycosphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF48">
        <property type="entry name" value="BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="83"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="198"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358670.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="37709" checksum="C61D6B8CF082AA17" modified="2020-08-12" version="1">MFVRIPYRLHIYDLLKTIGIVTLIVYLALPFRFASHYDYIDGAHIEGALLPQVTLNVSLQQTFECSYTDIIANNRFVYSSAYYHETVHHAEEIWPGGEFRPEQCQARYSTAIIVPYRQREEQLRAFLTYMHNYLRQQLIHYRIFLVEQYDQKPFNRAMLLNIGAKVAAEYGFPCLILHDVDLMPLNSGQMYACVETPRHMCPALDHWRFHLPYQGLFGGVVAITTLQFKQINGMSNVYHGWGGEDDDLYVRIMDEGIGICRFAPEYSEYTMLKHKPENPNEHRRALLQAAKLRRFMDGLSSLVYKEVERRMHSLFTHILVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP64</accession>
    <name>A0A6I8UP64_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Small ribosomal subunit protein uS14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">40S ribosomal protein S29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RpS29</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the 40S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358583.2">
        <property type="nucleotide sequence ID" value="XM_001358546.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP64"/>
    <dbReference type="SMR" id="A0A6I8UP64"/>
    <dbReference type="FunCoup" id="A0A6I8UP64">
        <property type="interactions" value="1063"/>
    </dbReference>
    <dbReference type="GeneID" id="4801504"/>
    <dbReference type="KEGG" id="dpo:4801504"/>
    <dbReference type="InParanoid" id="A0A6I8UP64"/>
    <dbReference type="OMA" id="HCFREIA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081106">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004424">
        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF2">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 13" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001358583.2"/>
        </source>
    </evidence>
    <sequence length="56" mass="6591" checksum="41D90F9793C08F8E" modified="2020-08-12" version="1">MGFATLWYSHPRKYGQGSRCCRACSNRHGLIRKYGLNICRQCFREYANDIGFKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP66</accession>
    <name>A0A6I8UP66_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calcyphosin-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4801423</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358519.4">
        <property type="nucleotide sequence ID" value="XM_001358482.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP66"/>
    <dbReference type="FunCoup" id="A0A6I8UP66">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="4801423"/>
    <dbReference type="KEGG" id="dpo:4801423"/>
    <dbReference type="InParanoid" id="A0A6I8UP66"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076212">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="85"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358519.4"/>
        </source>
    </evidence>
    <sequence length="219" mass="24711" checksum="62D8017FDFED64E0" modified="2020-08-12" version="1">MESNAKMQRENGFTKEANLQNLAKRELANGLDKDPIYKLRLQCFSRGATGILGLSRTFRVMDDDGSKTLSAEEFKKGISDVGLDVSESEISDMFTRFDADGSGTINMTEFLDKLRPPMNEGRLSIIEKAFDKMDANDDGQITETDLMNLYSVKDHPKYLSGEMTKSEILREFLKNFEAGAPNPDGIVTKEEFINYYSTISASIDNDAYFDLVMRRAYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPR0</accession>
    <name>A0A6I8UPR0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein PDF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Pdf</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arthropod PDH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358766.1">
        <property type="nucleotide sequence ID" value="XM_001358729.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPR0"/>
    <dbReference type="FunCoup" id="A0A6I8UPR0">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="GeneID" id="4801721"/>
    <dbReference type="KEGG" id="dpo:4801721"/>
    <dbReference type="InParanoid" id="A0A6I8UPR0"/>
    <dbReference type="OMA" id="MPDEERY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079634">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009396">
        <property type="entry name" value="Pigment_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06324">
        <property type="entry name" value="Pigment_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0027">Amidation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026078560" evidence="5">
        <location>
            <begin position="22"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001358766.1"/>
        </source>
    </evidence>
    <sequence length="97" mass="11057" checksum="6963F9728A7A8FBC" modified="2020-08-12" version="1">MAQYALTLAMLIAALCSRCELSRTMSLPDEERYVRKEYNRDLMDWFNTVGQLAPNQGVPLCRYPYIADNSLSIPTRKRNSEIINSLLSLPKNMNEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQC6</accession>
    <name>A0A6I8UQC6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HEAT repeat-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4802298</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear import and ribosome assembly adapter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359242.6">
        <property type="nucleotide sequence ID" value="XM_001359205.6"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQC6"/>
    <dbReference type="FunCoup" id="A0A6I8UQC6">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802298"/>
    <dbReference type="InParanoid" id="A0A6I8UQC6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052616">
        <property type="entry name" value="SYO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057990">
        <property type="entry name" value="TPR_SYO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25567">
        <property type="entry name" value="TPR_SYO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SYO1-like TPR repeats" evidence="3">
        <location>
            <begin position="381"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="302"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359242.6"/>
        </source>
    </evidence>
    <sequence length="627" mass="69884" checksum="F1BA4A3CA1FD96A3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQR2</accession>
    <name>A0A6I8UQR2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD repeat-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4802380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat WDR3/UTP12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359309.2">
        <property type="nucleotide sequence ID" value="XM_001359272.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQR2"/>
    <dbReference type="FunCoup" id="A0A6I8UQR2">
        <property type="interactions" value="2553"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802380"/>
    <dbReference type="InParanoid" id="A0A6I8UQR2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001663">
        <property type="entry name" value="GG22837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001830">
        <property type="entry name" value="GM15299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000755">
        <property type="entry name" value="WD repeat-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25172">
        <property type="entry name" value="Beta-prop_WDR3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="148"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="480"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="577"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="619"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="661"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="5">
        <location>
            <begin position="783"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359309.2"/>
        </source>
    </evidence>
    <sequence length="922" mass="103218" checksum="E50F2F667C4CFA80" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQX3</accession>
    <name>A0A6I8UQX3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Iron-sulfur cluster assembly scaffold protein IscU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">IscU</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359512.1">
        <property type="nucleotide sequence ID" value="XM_001359475.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQX3"/>
    <dbReference type="SMR" id="A0A6I8UQX3"/>
    <dbReference type="FunCoup" id="A0A6I8UQX3">
        <property type="interactions" value="1577"/>
    </dbReference>
    <dbReference type="GeneID" id="4802628"/>
    <dbReference type="KEGG" id="dpo:4802628"/>
    <dbReference type="InParanoid" id="A0A6I8UQX3"/>
    <dbReference type="OMA" id="SMVTEMV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082049">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000001">
        <property type="entry name" value="Iron-sulfur cluster assembly scaffold protein IscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISCU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="3">
        <location>
            <begin position="26"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359512.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="16606" checksum="77F9D1BA78391484" modified="2020-08-12" version="1">MSLVRNSSKLLVSQLKRVQSVPVALYHENVVEHYENPRNVGSLDKKDTTVGTGLVGAPACGDVMKLQIKVDDNGKIIDAKFKTFGCGSAIASSSLATEWVKGKSIDEAGQLKNTDIAKELRLPPVKLHCSMLAEDAIKAALADYKVKQQKKAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URB7</accession>
    <name>A0A6I8URB7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U6 snRNA phosphodiesterase</fullName>
            <ecNumber evidence="6">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4802702</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3'-end uridylyl-uridine-RNA = a 3'-end 2',3'-cyclophospho-uridine-RNA + uridine</text>
            <dbReference type="Rhea" id="RHEA:46052"/>
            <dbReference type="Rhea" id="RHEA-COMP:17384"/>
            <dbReference type="Rhea" id="RHEA-COMP:17385"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:85643"/>
            <dbReference type="ChEBI" id="CHEBI:85644"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:46053"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the 2H phosphoesterase superfamily. USB1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001359569.2">
        <property type="nucleotide sequence ID" value="XM_001359532.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URB7"/>
    <dbReference type="FunCoup" id="A0A6I8URB7">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802702"/>
    <dbReference type="InParanoid" id="A0A6I8URB7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990838">
        <property type="term" value="F:poly(U)-specific exoribonuclease activity, producing 3' uridine cyclic phosphate ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034477">
        <property type="term" value="P:U6 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03040">
        <property type="entry name" value="USB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359569.2"/>
        </source>
    </evidence>
    <sequence length="253" mass="28396" checksum="FCAAEEA0FA5AB16E" modified="2020-08-12" version="1">MALVNYEGSGSSSSEDDPPNLIFNTLKRSAALPTAVTLLGPKKAKPVEEDEVVDDPALHGGRIRSFKHERGNWATYVYVPALACAEQLEEFQEEAIACLAPDVQMQANEPLHLSLSRTVVLQYHQIDEFSRSLQVALNSSTGFASTLQGLKIYTNEERTRTFLAVQLDGAFTEKVLSILQPIDKVMHDYRLPKFNAPPSFHVSLLWCVGDHEELLNKKLKELRVLLEDQDTLQLAVNEIHCKCGKKDFTYKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URK2</accession>
    <name>A0A6I8URK2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Arrestin domain-containing protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4802975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359785.3">
        <property type="nucleotide sequence ID" value="XM_001359748.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URK2"/>
    <dbReference type="FunCoup" id="A0A6I8URK2">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802975"/>
    <dbReference type="InParanoid" id="A0A6I8URK2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF167">
        <property type="entry name" value="ARRESTIN C-TERMINAL-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="3">
        <location>
            <begin position="182"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359785.3"/>
        </source>
    </evidence>
    <sequence length="414" mass="46622" checksum="E3D54C9BF6602D2A" modified="2020-08-12" version="1">MNYRCDMQLDSPSGVYRAGETVNGHIYLTLTERALIKAISLEANGFAATGWLKPQKAGKPKKSQQQNLQVEKQPLAFENRVDYFAKIDYFVGSEASQPQIIEAGTYSYGFHVKLPKNCPSSHEGAHGHIRYTLQLLIHSSADRPIDVIHTRQLQVYSESELCKESRIGEVQCLEQTPRMKFWQKPLQLNLQIPRQGYSPGAGISVHVKLHNPQQLLLTEVVYRLNMVSTYVGLQTNKPKRRDIKVERQTLLSSGHELVSQPRSELEHFQHLHMLQVPQTPATLASAACACLQINYEVEVLVRTQQEKRSIMAQIPITIGNVTPPCPNSIRSMDPMNASAPEQTPTPGAATKLAPDFSVSMTSLASNFREAEFMTATNLNKNNKHALSGEQLDFKPRYLYYELDQNQTQEEIVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URT0</accession>
    <name>A0A6I8URT0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER-bound oxygenase mpaB/mpaB'/Rubber oxygenase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4803087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359886.4">
        <property type="nucleotide sequence ID" value="XM_001359849.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URT0"/>
    <dbReference type="KEGG" id="dpo:4803087"/>
    <dbReference type="InParanoid" id="A0A6I8URT0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018713">
        <property type="entry name" value="MPAB/Lcp_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37159">
        <property type="entry name" value="GH11867P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37159:SF1">
        <property type="entry name" value="GH11867P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09995">
        <property type="entry name" value="MPAB_Lcp_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="ER-bound oxygenase mpaB/mpaB'/Rubber oxygenase catalytic" evidence="3">
        <location>
            <begin position="79"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359886.4"/>
        </source>
    </evidence>
    <sequence length="386" mass="44621" checksum="AE26098B07EC953D" modified="2020-08-12" version="1">MTNGVSTDSGEHESSASGKMHSAQAPNNGHNDAEAEQYLEHLLKDGSQEGDSGSELELPPWFDEQLFKRAQGYFSTYRFVMNAGMLAGLISVLAVPSILRVLACTRQSSTAFTAYRRYVRTIFHTQSWYNNSVADRKSKFWTSIAAVRQAHARSSHACARRGAGQITQKDLALTQFGFIGFITLGAHRIQLYDNDFLEATTHMWRVLGYLLGIKDEYNICGRNWAESKTRLDIVMRKVYEPALEHTTEEFYRMTEALIQGLWHVNTMLSVGANIFFTKRLACVKGYEYYSFDHRVGEKRDPQQRLYYYDLKLWDRLIVSYGLFIVTFLHKYAIVRWYLNFRVWLVDLLTYYLPYIAIWKFGVKSAYVRIFRKGGEAQEFSLGFKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URW3</accession>
    <name>A0A6I8URW3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Plancitoxin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DNaseII</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359949.3">
        <property type="nucleotide sequence ID" value="XM_001359912.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URW3"/>
    <dbReference type="FunCoup" id="A0A6I8URW3">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803163"/>
    <dbReference type="InParanoid" id="A0A6I8URW3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09120">
        <property type="entry name" value="PLDc_DNaseII_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09121">
        <property type="entry name" value="PLDc_DNaseII_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026079516" evidence="3">
        <location>
            <begin position="20"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359949.3"/>
        </source>
    </evidence>
    <sequence length="370" mass="41324" checksum="D26328FC66E14FC4" modified="2020-08-12" version="1">MRSLCVLLLLIGISSQCLPAKADGSKKVSCKDEQGNDVDWWHLYKLPKHYQQNDLGKDVSGLRYLYVTSNSYDNWQLSGKRVGDADSLPAQTINALNVDPTHTLLAAYNDQWPNGTVLSTGGHAKGLVGSDGETGVWIVHSVPKYPTLPEYTYPTTGEHYAQSMLCITVKAVDLEKVGQVLVYNEPHFYYERNPLLQRSDLLFPSLERAMHGQWRTEAPFQKDLELRSLDGKKFRLFGKSGRANVELYADVVAPALDVSLFVEAWRDGAGNLPNRCGSNDKVFNVESVANLELSVDFKTTQDHSKWAVSRPSGILLYHWRVGGGDWICVGDINRQETQLKRGGGTLCHKSSRVSNLYRNLVANYDKCAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USQ6</accession>
    <name>A0A6I8USQ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tubulin--tyrosine ligase-like protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TTLL12</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360322.2">
        <property type="nucleotide sequence ID" value="XM_001360285.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USQ6"/>
    <dbReference type="FunCoup" id="A0A6I8USQ6">
        <property type="interactions" value="1825"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803634"/>
    <dbReference type="InParanoid" id="A0A6I8USQ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057954">
        <property type="entry name" value="SET_TTL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027749">
        <property type="entry name" value="TTLL12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25556">
        <property type="entry name" value="SET_TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin--tyrosine ligase-like protein 12 SET-like" evidence="1">
        <location>
            <begin position="75"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360322.2"/>
        </source>
    </evidence>
    <sequence length="627" mass="73479" checksum="018350F3A06B2E5F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTS0</accession>
    <name>A0A6I8UTS0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein regulatory complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Coiled-coil domain-containing protein 65</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC4803840</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Plays a critical role in the assembly of N-DRC and also stabilizes the assembly of multiple inner dynein arms and radial spokes. Coassembles with DRC1 to form a central scaffold needed for assembly of the N-DRC and its attachment to the outer doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytoskeleton</location>
            <location evidence="8">Flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DRC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360493.4">
        <property type="nucleotide sequence ID" value="XM_001360456.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTS0"/>
    <dbReference type="FunCoup" id="A0A6I8UTS0">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803840"/>
    <dbReference type="InParanoid" id="A0A6I8UTS0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003352">
        <property type="term" value="P:regulation of cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039505">
        <property type="entry name" value="DRC1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039750">
        <property type="entry name" value="DRC1/DRC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625:SF0">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625">
        <property type="entry name" value="NYD-SP28 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14772">
        <property type="entry name" value="NYD-SP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein regulatory complex protein 1/2 N-terminal" evidence="15">
        <location>
            <begin position="27"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="464"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="410"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001360493.4"/>
        </source>
    </evidence>
    <sequence length="536" mass="62431" checksum="F1F8881C77B1BF4C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU27</accession>
    <name>A0A6I8UU27_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex I-B14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase B14 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ND-B14</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Required for proper complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Mammalian complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360938.2">
        <property type="nucleotide sequence ID" value="XM_001360901.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU27"/>
    <dbReference type="FunCoup" id="A0A6I8UU27">
        <property type="interactions" value="1343"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804364"/>
    <dbReference type="InParanoid" id="A0A6I8UU27"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20266">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045299">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016488">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B14 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="14">
        <location>
            <begin position="66"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001360938.2"/>
        </source>
    </evidence>
    <sequence length="154" mass="18382" checksum="AAB07D6D4E65A66D" modified="2020-08-12" version="1">MHRKTSVNIGRNYITNYFGKNNFAQKTETAMAGREAIKRAVQQVRPILSVDREEARKRALNLYKAWYRQIPYIVMDYDIPMTVEQCRSKLREQFVKDRHVTDIRVIDMLVIKGQMELKETVEIWKQKGHIMRYWKESQEPKPTDFLSKFVQGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUS9</accession>
    <name>A0A6I8UUS9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Epidermal growth factor receptor substrate 15-like 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Eps-15</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
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                <consortium name="RefSeq"/>
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        <source>
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</entry>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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            <strain evidence="15">MV-25-SWS-2005</strain>
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            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        <text evidence="2">Protein modification; protein ubiquitination.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000079">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000014">
        <property type="entry name" value="E3 ubiquitin-protein ligase SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000125">
        <property type="entry name" value="E3 ubiquitin-protein ligase Smurf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
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    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF395">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="12">
        <location>
            <begin position="165"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="12">
        <location>
            <begin position="527"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="12">
        <location>
            <begin position="575"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="13">
        <location>
            <begin position="758"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="141"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="186"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="617"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="355"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="496"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="624"/>
            <end position="659"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="660"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="1064"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079482"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001361200.4"/>
        </source>
    </evidence>
    <sequence length="1096" mass="118534" checksum="0D433424CE8558BF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXD6</accession>
    <name>A0A6I8UXD6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acylphosphatase-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6902860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the acylphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132596.2">
        <property type="nucleotide sequence ID" value="XM_002132560.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXD6"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002132596.2"/>
        </source>
    </evidence>
    <sequence length="108" mass="12013" checksum="5EA7F843A14996FA" modified="2020-08-12" version="1">MSKAKEDASQIIMTCDFEIKGQIPKEAFEMFAAAQAQILGLRGYIVQVSEECFKGQLQGEGRVIEAFKQLILAAAEYVAAVKEFIIQNLKAIDEYTYPAFEVKAKGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYU3</accession>
    <name>A0A6I8UYU3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">RING-box protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC6902120</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133651.2">
        <property type="nucleotide sequence ID" value="XM_002133615.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYU3"/>
    <dbReference type="KEGG" id="dpo:6902120"/>
    <dbReference type="InParanoid" id="A0A6I8UYU3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UYU3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16485">
        <property type="entry name" value="mRING-H2-C3H2C2D_RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000273">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="84"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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</entry>
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            <dbReference type="RefSeq" id="XP_002134038.2"/>
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    <sequence length="302" mass="32638" checksum="EE48D30B2CE974ED" modified="2020-08-12" version="1">MQACKLFLSLLLAMHCTQALLAACVCSEKGSDYCQSCQGPTIVRPKPRYYEPSDVNLSPIGDNCWCSKQLIEPAQLPKTCGKTTPCKPTCGCQQSDDSYSSGSSSSSSYGNIAYAAEKAEDNSPAADPISVSLAAQAGKAINVPEAKLAFGFAQKPVDGVQKVQFSNVPEENLFKLKSEVITLKKPNYVAKQEEEEEYIEEEEQQQSDDEPAPQLTYKQLGYITEQFKNPKFKKITSSRSSYGSSSYGSSSYGSSSYASKSASSDGYGKVAGKVSVDCGFKPGRITSYRNANRQEPDAGSCY</sequence>
</entry>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0E6</accession>
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    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134805.3">
        <property type="nucleotide sequence ID" value="XM_002134769.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0E6"/>
    <dbReference type="KEGG" id="dpo:6900413"/>
    <dbReference type="InParanoid" id="A0A6I8V0E6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF106">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="90"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="150"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="222"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002134805.3"/>
        </source>
    </evidence>
    <sequence length="350" mass="39211" checksum="4ED59265C0B3EC90" modified="2020-08-12" version="1">MDRSITQTVPKGLLYLLMCLVTAGGFTMEERCQYWAGPGFIGDSNDCQAWGYCKDNKLESSNRCKIGLLYDFRDGSCKLPSEVNCQTTLTDACAITRPGEYAADPTDCARYIKCGQTDSDDEVERICGDYQLFSNQLQTCVADTQGCPQDYPCAHMQDSTLLADPNSCRSYLRCKNGLSTKFKCSSRRYFNRQTGQCQASLPDECESDDEIDTPAIPPATNADVCLRLYDQAKTRGVQLLTDSMTCHGYYRCSSPYALGEWASCPPGTHFQWWRQRCASPEETSCPYDRCANLRSRFVAILDTGCRDYTLCQDQQSVGSDTCPPIHPYFDETLGQCGKHFPNFRVCYIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0X3</accession>
    <name>A0A6I8V0X3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Serine protease 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6900727</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135090.2">
        <property type="nucleotide sequence ID" value="XM_002135054.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0X3"/>
    <dbReference type="KEGG" id="dpo:6900727"/>
    <dbReference type="InParanoid" id="A0A6I8V0X3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0X3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026126995" evidence="9">
        <location>
            <begin position="19"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="24"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002135090.2"/>
        </source>
    </evidence>
    <sequence length="258" mass="28490" checksum="785133D01CDDE574" modified="2020-08-12" version="1">MLIVLWLLLVRDFIVGEANVLPRIVNGKLADQRQFPYAVQVEAGNNRTVLCGGSIIANDWVLTAGHCTENATWVTLYYGSVWTKHYPLMTLEAKTHVFQHPQFRSVSGDIINDIALIRTEWVEFSDRIQSIALPTEPKYGYNNYVGQWATVAGWGQLHDHGSGSVRLFWATLKVLDVSVCRRRYGNKLVGLLCAQSGDAQSTCYGDSGGPLVLNGENKLIGVFAFLSYLGCEVGDPVGFSDVSRYLGWIRSVSGIGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2H9</accession>
    <name>A0A6I8V2H9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclin-dependent kinase inhibitor 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135584.2">
        <property type="nucleotide sequence ID" value="XM_002135548.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2H9"/>
    <dbReference type="KEGG" id="dpo:6900255"/>
    <dbReference type="InParanoid" id="A0A6I8V2H9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685:SF2">
        <property type="entry name" value="825-OAK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685">
        <property type="entry name" value="825-OAK-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026101382" evidence="1">
        <location>
            <begin position="18"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135584.2"/>
        </source>
    </evidence>
    <sequence length="151" mass="15047" checksum="B5E17D09A9131AAE" modified="2020-08-12" version="1">MFKYAVVICALIACVAAKPGLLHAPLAAPLFAAPAPVVTAASSQVVARTFNGIATAPVIAQVPVAPAPVPVVRTVAAPFAAPLAAAPLPAPIVRTVTGPVPAPFAAQYTASFGAAYAAPFAHPYGTPVIAKYSAPLAYAPAPLNYAAPALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2U4</accession>
    <name>A0A6I8V2U4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137438.2">
        <property type="nucleotide sequence ID" value="XM_002137402.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2U4"/>
    <dbReference type="KEGG" id="dpo:6897278"/>
    <dbReference type="InParanoid" id="A0A6I8V2U4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026188306" evidence="2">
        <location>
            <begin position="23"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137438.2"/>
        </source>
    </evidence>
    <sequence length="324" mass="36005" checksum="9A76ACFC046DE193" modified="2020-08-12" version="1">MKWSSSLLWLAALCASYGGICGRDPHGDQWQDMSELGRQVEEIIRSEMSSGATSEEVLNNLHTSSNEVIQRAGFVAINLANKVQIAITELIASFVESVGNALGELQSFLQKERNTYRSQALQDATGMLTQLNAVILDLEASLERINGQQANNDQWELQTAEEWKLWAAVLVSNVRQSTEGAGTDISKAKNITQGLNTRYGLRLHACQEGFIWAHTRFNLELQETLEIAQNSTDVLIQATVNCQVVGDECRNAVRKALNGIIEVPVQLDRFRDKAEHLLVVIGESNQCLSETLEEYAGQRLKVESQLQDIIEQYGNTVSPETVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3R3</accession>
    <name>A0A6I8V3R3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6897869</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c oxidase IV family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137971.2">
        <property type="nucleotide sequence ID" value="XM_002137935.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3R3"/>
    <dbReference type="FunCoup" id="A0A6I8V3R3">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897869"/>
    <dbReference type="InParanoid" id="A0A6I8V3R3"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00922">
        <property type="entry name" value="Cyt_c_Oxidase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.442.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.442.10">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013288">
        <property type="entry name" value="Cyt_c_oxidase_su4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004203">
        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF10">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01873">
        <property type="entry name" value="CYTCOXIDASE4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002137971.2"/>
        </source>
    </evidence>
    <sequence length="187" mass="21793" checksum="98BDBDA4D0E6CAA5" modified="2020-08-12" version="1">MSVMRALGLRLHNQFQQVLPQRGAVMAMMRRGTHHDGTRLMSGDREVVGYGVNGNPIYIDCVEFPFPAIRYREVTAELCAVREKELGDWKALSIQDKKLLYRHSFCQTYAEFQHFTPDWKLVIGVGFWSAAIGLLISLSYYFKLYDPVPETYAEDRRQAQLRRIIQLQMNPITGLSSKWCYHTNKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3X9</accession>
    <name>A0A6I8V3X9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein Ir100a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ir100a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137546.2">
        <property type="nucleotide sequence ID" value="XM_002137510.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3X9"/>
    <dbReference type="FunCoup" id="A0A6I8V3X9">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897394"/>
    <dbReference type="InParanoid" id="A0A6I8V3X9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000289">
        <property type="entry name" value="Ionotropic receptor 10a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF38">
        <property type="entry name" value="IONOTROPIC RECEPTOR 100A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026143323" evidence="9">
        <location>
            <begin position="21"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="571"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002137546.2"/>
        </source>
    </evidence>
    <sequence length="599" mass="68123" checksum="7882FE25AA16C9D5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3Z0</accession>
    <name>A0A6I8V3Z0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spondin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">F-spondin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6898178</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138249.2">
        <property type="nucleotide sequence ID" value="XM_002138213.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3Z0"/>
    <dbReference type="FunCoup" id="A0A6I8V3Z0">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898178"/>
    <dbReference type="InParanoid" id="A0A6I8V3Z0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08544">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000135">
        <property type="entry name" value="F-spondin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4060:FF:000005">
        <property type="entry name" value="GD10853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2130:FF:000002">
        <property type="entry name" value="Putative Spondin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000160">
        <property type="entry name" value="spondin-1"/>
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        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
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        <property type="entry name" value="Reeler_dom"/>
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        <property type="entry name" value="Reeler_sf"/>
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        <property type="entry name" value="Spondin/Thrombospondin_T1"/>
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        <property type="entry name" value="Spondin_N"/>
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        <property type="entry name" value="TSP1_rpt"/>
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        <property type="entry name" value="TSP1_rpt_sf"/>
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    <dbReference type="NCBIfam" id="NF038123">
        <property type="entry name" value="NF038123_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPONDIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02014">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06468">
        <property type="entry name" value="Spond_N"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF19028">
        <property type="entry name" value="TSP1_spondin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51019">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51020">
        <property type="entry name" value="SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026019713" description="Spondin-1" evidence="12">
        <location>
            <begin position="23"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="Reelin" evidence="14">
        <location>
            <begin position="8"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin" evidence="15">
        <location>
            <begin position="176"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="13">
        <location>
            <begin position="653"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002138249.2"/>
        </source>
    </evidence>
    <sequence length="864" mass="97930" checksum="ED86C8520B89F518" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V409</accession>
    <name>A0A6I8V409_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Calreticulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Calr</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 17">Belongs to the calreticulin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_002137581.4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="dpo:6897468"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Calret/calnex"/>
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        <property type="entry name" value="Calret/calnex_CS"/>
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        <property type="entry name" value="Calreticulin"/>
    </dbReference>
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        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF2">
        <property type="entry name" value="CALRETICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002356">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
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    </dbReference>
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        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00805">
        <property type="entry name" value="CALRETICULIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 14 17" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14 17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026370276" description="Calreticulin" evidence="17">
        <location>
            <begin position="20"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="207"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="350"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="207"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="391"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="105"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR002356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_002137617.4"/>
        </source>
    </evidence>
    <sequence length="405" mass="46751" checksum="150C44DD4272D8CE" modified="2020-08-12" version="1">MCYRITILALLAIVGLINAHIYLEEKFDNGNWDDTWIYSKHPGKEFGKFAHSSGSFFNDAEADKGIQTSQDARFYAVSRKFDAFSNEDKPLVVQFSVKHEQNIDCGGGYVKLFDCSLDQTDMHGESPYEIMFGPDICGPGTKKVHVIFSYKGKNHLINKDVRCKDDVYTHFYTLIVKPDNTYEVLIDNEKVESGNLEDDWDFLAPKKIKDPNAKKPEDWDDKPTIADPDDKKPEDWDKSEHIPDPDATKPEDWDDEMDGEWEPPMVDNPEYKGEWQPKQLDNPNYKGAWEHPEIDNPEYIPDNKLYLRKEVCKLGFDLWQVKSGTIFDNVLITDDIELAFNAAANVKNTQAGEKKMKEIQDEVQRKKDEEEAKKASDKDDEEEDDDEDEKDESKPDKDQSEHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4M5</accession>
    <name>A0A6I8V4M5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 216</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6897886</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137988.1">
        <property type="nucleotide sequence ID" value="XM_002137952.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4M5"/>
    <dbReference type="FunCoup" id="A0A6I8V4M5">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="GeneID" id="6897886"/>
    <dbReference type="KEGG" id="dpo:6897886"/>
    <dbReference type="InParanoid" id="A0A6I8V4M5"/>
    <dbReference type="OMA" id="ITFARAN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248884">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019184">
        <property type="entry name" value="Uncharacterised_TM-17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531:SF0">
        <property type="entry name" value="GEO07735P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531">
        <property type="entry name" value="GEO07735P1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09799">
        <property type="entry name" value="Transmemb_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002137988.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="16997" checksum="8F9281757C2C2C9E" modified="2020-08-12" version="1">MPPKRPPPLVPKQPNPSMNYEVLIYLNSFYFGMFACCEMAMGLLKAINLSYTGHTLAMDSAVMVSFILLESIRLIMGRKSSLADRGWSAILSVFMTAPCFLGVSYLLLLQTYRLRLEYCLCTLQIALYFTEVWYAIVFVFSLCRPVTYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V695</accession>
    <name>A0A6I8V695_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4803253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736932.1">
        <property type="nucleotide sequence ID" value="XM_003736884.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V695"/>
    <dbReference type="GeneID" id="4803253"/>
    <dbReference type="KEGG" id="dpo:4803253"/>
    <dbReference type="InParanoid" id="A0A6I8V695"/>
    <dbReference type="OMA" id="LSMPRWQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076135">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_003736932.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="29278" checksum="3EB4923245F07586" modified="2020-08-12" version="1">MWQPRRRRCSKEWCLRNAQPKPSIPPFKPEPTRWAKPGPMNLDDWVNFYKWCQVNAAPIERPPIKKEPPPPPTPERVPRMAEAPKAPIVGWTRCESGGNRDEQDAFSRLSSPRWRRGKYQPPPPEVFAYRPRLAYQPRPMPEPGRPANKIKVPCCFQHDDVESEFWATVRFPVSKNARSTMPSRKDCELSRPRAVPPKPHCPLPPQNAYLMPKRKKMTARQWRAHQQRLKILAQPSERILAELPCFCDRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7U9</accession>
    <name>A0A6I8V7U9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dedicator of cytokinesis protein 11 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Ziz</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035536.2">
        <property type="nucleotide sequence ID" value="XM_015180050.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V7U9">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V7U9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V7U9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08697">
        <property type="entry name" value="C2_Dock-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11694">
        <property type="entry name" value="DHR2_DOCK_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13267">
        <property type="entry name" value="PH_DOCK-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037809">
        <property type="entry name" value="C2_Dock-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF26">
        <property type="entry name" value="ZIZIMIN, ISOFORM K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="295"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="5">
        <location>
            <begin position="800"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="6">
        <location>
            <begin position="1819"/>
            <end position="2250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1563"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2290"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1578"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="2291"/>
            <end position="2305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
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<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VA25</accession>
    <name>A0A6I8VA25_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Alpha-mannosidase</fullName>
            <ecNumber evidence="10">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LManVI</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_015036952.2">
        <property type="nucleotide sequence ID" value="XM_015181466.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VA25"/>
    <dbReference type="FunCoup" id="A0A6I8VA25">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117183164"/>
    <dbReference type="InParanoid" id="A0A6I8VA25"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10810">
        <property type="entry name" value="GH38N_AMII_LAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000018">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1360:FF:000004">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026377359" description="Alpha-mannosidase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="11">
        <location>
            <begin position="359"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015036952.2"/>
        </source>
    </evidence>
    <sequence length="1010" mass="115191" checksum="F4F91409A06C2743" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBG3</accession>
    <name>A0A6I8VBG3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Synapse-associated protein of 47 kDa isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">Sap47</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037746.2">
        <property type="nucleotide sequence ID" value="XM_015182260.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037753.2">
        <property type="nucleotide sequence ID" value="XM_015182267.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBG3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VBG3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3970.10:FF:000001">
        <property type="entry name" value="synapse-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="3">
        <location>
            <begin position="299"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="488"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="520"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015037746.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015037753.2"/>
        </source>
    </evidence>
    <sequence length="560" mass="58348" checksum="D95F7136248E452E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBV8</accession>
    <name>A0A6I8VBV8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein Gfrl isoform X8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Gfrl</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GDNFR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037436.2">
        <property type="nucleotide sequence ID" value="XM_015181950.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VBV8">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:13036379"/>
    <dbReference type="InParanoid" id="A0A6I8VBV8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057681">
        <property type="entry name" value="DUF7921"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059035">
        <property type="entry name" value="Fn1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF12">
        <property type="entry name" value="GLIAL CELL LINE-DERIVED NEUROTROPHIC FAMILY RECEPTOR-LIKE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25537">
        <property type="entry name" value="DUF7921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25868">
        <property type="entry name" value="Fn1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="95"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="194"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="667"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="776"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="282"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="881"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="311"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="336"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="881"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="896"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="928"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="979"/>
            <end position="993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015037436.2"/>
        </source>
    </evidence>
    <sequence length="1330" mass="145544" checksum="C55E6A9B5D206ADF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VC63</accession>
    <name>A0A6I8VC63_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Transcription factor Ouib isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">nom</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015038238.2">
        <property type="nucleotide sequence ID" value="XM_015182752.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VC63"/>
    <dbReference type="InParanoid" id="A0A6I8VC63"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000770">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="15">
        <location>
            <begin position="4"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="209"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015038238.2"/>
        </source>
    </evidence>
    <sequence length="345" mass="39648" checksum="B67984FF70CC922F" modified="2020-08-12" version="1">MLINLCRVCGKSNICPKALPLFEPSNRKLLRYIYDLTGIRVSFSLLLCHSNSPHMVCFCCQMDIKTANVFRRQCIKVQQKWSAMEKKTEDQTPKIRRSVRCSRRRPVLHLPVEPLQIVDVVMKTEDEFIQAPSVNNEVDQLAWTSAEQVAIHTEGSPDETDHDSSSSSDKDYSAQPKLQLHKCNLCGIVKNNKASLVRHKYTHTGERPHPCKECPKAFLSGNELRAHMLTQHTEEPPFPCRYCERKYFSSIGRKKHERVHTNERPFVCEQCGKAFTRGCIMKQHMLSHTGQRMFNCDICNRSFTLKKHLVTHYISNTHKLNAAAQKVVLPRGENCASNKQQNTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDK2</accession>
    <name>A0A6I8VDK2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rap guanine nucleotide exchange factor 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Epac</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAPGEF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015039065.1">
        <property type="nucleotide sequence ID" value="XM_015183579.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDK2"/>
    <dbReference type="FunCoup" id="A0A6I8VDK2">
        <property type="interactions" value="328"/>
    </dbReference>
    <dbReference type="GeneID" id="6898079"/>
    <dbReference type="InParanoid" id="A0A6I8VDK2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245946">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VDK2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04437">
        <property type="entry name" value="DEP_Epac"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000002">
        <property type="entry name" value="Rap guanine nucleotide exchange factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000194">
        <property type="entry name" value="Rap guanine nucleotide exchange factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000468">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000228">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.8.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
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    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="DEP_dom"/>
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    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
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        <property type="entry name" value="Ras-like_GEF"/>
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    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
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    <dbReference type="InterPro" id="IPR019804">
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    <dbReference type="InterPro" id="IPR023578">
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    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
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        <property type="entry name" value="RmlC-like_jellyroll"/>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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        <property type="entry name" value="EXCHANGE PROTEIN DIRECTLY ACTIVATED BY CAMP, ISOFORM E"/>
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        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
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        <property type="entry name" value="cNMP_binding"/>
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        <property type="entry name" value="RasGEFN"/>
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        <property type="entry name" value="cAMP-binding domain-like"/>
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        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="51"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="7">
        <location>
            <begin position="209"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="6">
        <location>
            <begin position="352"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="9">
        <location>
            <begin position="486"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="8">
        <location>
            <begin position="660"/>
            <end position="742"/>
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    </feature>
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        <location>
            <begin position="767"/>
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        <location>
            <begin position="161"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010829"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50009"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015039065.1"/>
        </source>
    </evidence>
    <sequence length="1006" mass="114070" checksum="364FCB838672EE56" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFG4</accession>
    <name>A0A6I8VFG4_DROPS</name>
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        <submittedName>
            <fullName evidence="14">Probable ascorbate-specific transmembrane electron transporter 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nemy</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015040189.1">
        <property type="nucleotide sequence ID" value="XM_015184703.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043205">
        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
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        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF24">
        <property type="entry name" value="NO EXTENDED MEMORY, ISOFORM A"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
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    </dbReference>
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        <property type="entry name" value="B561"/>
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    </dbReference>
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        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="12">
        <location>
            <begin position="72"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015040189.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="34182" checksum="D7237DF3AF845041" modified="2020-08-12" version="1">MGKKFYKLWSLFKKRSPSNMKTIDKPFIDELNRMLFNQTRTGRLPKNERYEEDPDNAWNCCSWCEYLLIVILSTILLVGVLVLTLFWVMFYRDGFAWSESPKQQFNLHPILMIAGFVTLSGFSILIYRLCRCVKHIYVKLIHMFFHAIAIPCIALGFLSVFDSHEALQKVNFYSLHSWLGFVTMGMFVLQFVIGFFSFLVMLCCENKTYSCRSAMVPIHASLGLANFWLAIATSVTGLIEKERETVAETNVSAENKLIEHYITSAIGITLIFIGIIVTFAVRRSNAPASAKVYVTERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJP7</accession>
    <name>A0A6I8VJP7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lysozyme</fullName>
            <ecNumber evidence="2">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4813610</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="2"/>
    <dbReference type="RefSeq" id="XP_015042752.2">
        <property type="nucleotide sequence ID" value="XM_015187266.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VJP7"/>
    <dbReference type="FunCoup" id="A0A6I8VJP7">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VJP7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16890">
        <property type="entry name" value="lyz_i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008597">
        <property type="entry name" value="Invert_lysozyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11195">
        <property type="entry name" value="DESTABILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11195:SF22">
        <property type="entry name" value="LYSOZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05497">
        <property type="entry name" value="Destabilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51909">
        <property type="entry name" value="LYSOZYME_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="4" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="137"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="153"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="189"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608597-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015042752.2"/>
        </source>
    </evidence>
    <sequence length="257" mass="27711" checksum="4939BADC748AA73A" modified="2020-08-12" version="1">MDRTSSSSVAELGEGDGAAGGAKATGTAHQEYAEALSLSSQPRKRRWNIRSCSWSTLDLLTVAAIVTALLLILGAIYMHLKQRPHLGRLHITRKEKEKELVAVDTESLPVSLDVAAGVAAQSITTFRPSVLEPSGDCLPCIAVAATDNHPAMCGGRPCGIYRISWLYWMDAQGTSDPDSALTQADYEECVPVASCAEGLVRSYVKRFGYDCDGDGLIGCRDHVMLHLNGPTGCRMGKPLAALAERRMNKCFTQKNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VM20</accession>
    <name>A0A6I8VM20_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaminyl-peptide cyclotransferase</fullName>
            <ecNumber evidence="4">2.3.2.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">isoQC</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as a glutaminyl-peptide cyclotransferase. Responsible for the biosynthesis of pyroglutamyl peptides. Might be more efficient in the conversion of tri and tetrapeptides in vitro. Might have a relative preference for substrates containing hydrophobic amino acids in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl-[peptide] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23652"/>
            <dbReference type="Rhea" id="RHEA-COMP:11736"/>
            <dbReference type="Rhea" id="RHEA-COMP:11846"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="ChEBI" id="CHEBI:87215"/>
            <dbReference type="EC" id="2.3.2.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaminyl-peptide cyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.5" evidence="4"/>
    <dbReference type="RefSeq" id="XP_015044066.2">
        <property type="nucleotide sequence ID" value="XM_015188580.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VM20"/>
    <dbReference type="FunCoup" id="A0A6I8VM20">
        <property type="interactions" value="586"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VM20"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VM20">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03880">
        <property type="entry name" value="M28_QC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000029">
        <property type="entry name" value="Glutaminyl-peptide cyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037457">
        <property type="entry name" value="M28_QC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF6">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M28" evidence="13">
        <location>
            <begin position="128"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015044066.2"/>
        </source>
    </evidence>
    <sequence length="363" mass="41646" checksum="7547E44538997668" modified="2020-08-12" version="1">MRGLLNLNRGRHLVRQYSWMEAVKTMLAKPRKKIYNLGACFELADISQTSYNPSKLSESKFLEYSNLSNKLHLREAISHILIPRVVGSGNHSLVRQYIVQSLKDLDWHVELDSFHDTAPIMGQLHFHNIIATLNPDAERYLVLACHYDSKYMPKAEFLGATDSAVPCAMLLNLAQVLQEQLKPFKKSKLSLMLLFFDGEEAFEEWGPKDSIYGARHLAKRWQHEGKLDSIDMLVLLDLLGAPDPAFYSFFANTESWYMRLQSVETRLAQIELLERYASSGVSQRDPTRYFQSQAMRSSFIEDDHIPFLKRNVPVLHLIPVPFPTVWHTPDDNESVIDYATTDNLALIIRLFALEYLLGGDQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VML4</accession>
    <name>A0A6I8VML4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CLIP domain-containing serine protease</fullName>
            <ecNumber evidence="11">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">ea</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The clip domain consists of 35-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_033232300.1">
        <property type="nucleotide sequence ID" value="XM_033376409.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232301.1">
        <property type="nucleotide sequence ID" value="XM_033376410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VML4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000084">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.30:FF:000005">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022700">
        <property type="entry name" value="CLIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038565">
        <property type="entry name" value="CLIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12032">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00680">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51888">
        <property type="entry name" value="CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 11 16" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Clip" evidence="14">
        <location>
            <begin position="60"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="153"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033232300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033232301.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="46360" checksum="2AFA7EFE1E54B378" modified="2020-08-12" version="1">MLIVVRYSEAVCQTELLFIFGIRYICIFAQFRLNYTQCLIICPPEFYFPNEATQVPNYGRCITPNRERALCIHLEDCKYLYGVLTTSPLRDTDRLYLSRSQCGYTNGKVLICCLDRYRDTSPTTMQQSVQPNITNSNLLPLPGQCGNLLSNRIYGGVRTKIDEFPWMALIEYTKTAGKKGHHCGGSLISTRYVVTASHCVNGKALPADWRLTGVRLGEWDTATDPDCELDVRGMKDCAPAHLDVPVERTIPHPSYIPSSKNQVNDIALLRLGNQVEYTDFVRPICLPLDANLRSATFDGITMDVAGWGKTEEMSSSNLKLKAAVEGTRMDDCQAVYNRQDIMLEDTQMCAGGKEGIDSCRGDSGGPLIGLDTNKVNTYYFLAGVVSFGPTPCGLAGWPGVYTLVGKYVDWIQNTIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPH8</accession>
    <name>A0A6I8VPH8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232942.1">
        <property type="nucleotide sequence ID" value="XM_033377051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPH8"/>
    <dbReference type="KEGG" id="dpo:117183356"/>
    <dbReference type="InParanoid" id="A0A6I8VPH8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026131275" description="MD-2-related lipid-recognition domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21175" checksum="D14D11D5F3C8DB9D" modified="2020-08-12" version="1">MRVPIIPIRVLTFVAMILILHGSDGLFKYKNIKCKCYEKSYCEFKKCELKVLGRGIVGLNMHVQAKQLPITSVKAILTMFRQFSGYRPFLYNVTVDICGFLKNPKRFPFFSMVHDAIRNFSNFNHSCPYNDDIIIDRMVLNDEMISRMPVPNGFYKLLVAVATEGIWRGEVEAYVEVNLGYDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVP8</accession>
    <name>A0A6I8VVP8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Kazrin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7 8 9" type="primary">Liprin-gamma</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6 8">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="5 6 8">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="7 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 9">MV-25-SWS-2005</strain>
            <tissue evidence="7 9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361653.4">
        <property type="nucleotide sequence ID" value="XM_001361616.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040488.2">
        <property type="nucleotide sequence ID" value="XM_015185002.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234659.1">
        <property type="nucleotide sequence ID" value="XM_033378768.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234660.1">
        <property type="nucleotide sequence ID" value="XM_033378769.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234661.1">
        <property type="nucleotide sequence ID" value="XM_033378770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VVP8"/>
    <dbReference type="KEGG" id="dpo:4805212"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VVP8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd09564">
        <property type="entry name" value="SAM_kazrin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09567">
        <property type="entry name" value="SAM_kazrin_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09570">
        <property type="entry name" value="SAM_kazrin_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037614">
        <property type="entry name" value="Kazrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059089">
        <property type="entry name" value="Kazrin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037613">
        <property type="entry name" value="Kazrin_SAM_rpt_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037615">
        <property type="entry name" value="Kazrin_SAM_rpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037616">
        <property type="entry name" value="Kazrin_SAM_rpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776:SF1">
        <property type="entry name" value="KAZRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776">
        <property type="entry name" value="KAZRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25986">
        <property type="entry name" value="Kazrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="746"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="829"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="105"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="541"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1034"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="374"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1050"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1098"/>
            <end position="1109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361653.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015040488.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033234659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033234660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033234661.1"/>
        </source>
    </evidence>
    <sequence length="1186" mass="125882" checksum="0D9A0DBEAEEA39E5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VW61</accession>
    <name>A0A6I8VW61_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Liprin-alpha-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Liprin-alpha</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the liprin family. Liprin-alpha subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235193.1">
        <property type="nucleotide sequence ID" value="XM_033379302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VW61"/>
    <dbReference type="FunCoup" id="A0A6I8VW61">
        <property type="interactions" value="572"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VW61"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VW61">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:synapse organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat1"/>
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        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat2"/>
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        <property type="entry name" value="SAM_liprin-alpha1_2_3_4_repeat3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PTPRF interacting protein alpha 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PTPRF interacting protein alpha 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="LIP-1_CC2"/>
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        <property type="entry name" value="LIP-1_SAM_1"/>
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        <property type="entry name" value="LIP-1_SAM_3"/>
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        <property type="entry name" value="Liprin"/>
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        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
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        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LIPRIN-ALPHA, ISOFORM E"/>
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        <property type="entry name" value="LIP-1"/>
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        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="SAM_DOMAIN"/>
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        <location>
            <begin position="945"/>
            <end position="1011"/>
        </location>
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    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="1038"/>
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    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="1118"/>
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        <location>
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        <location>
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        <location>
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        <location>
            <begin position="35"/>
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        <location>
            <begin position="261"/>
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        <location>
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        <location>
            <begin position="639"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="204"/>
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        </location>
    </feature>
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        <location>
            <begin position="549"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="694"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="732"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007026"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033235193.1"/>
        </source>
    </evidence>
    <sequence length="1201" mass="134810" checksum="B5EA77A923ACF829" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXL6</accession>
    <name>A0A6I8VXL6_DROPS</name>
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        <recommendedName>
            <fullName evidence="3">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">LOC117184012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033379906.1"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
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    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033235797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="26873" checksum="9D9F2D5AAE0885BC" modified="2020-08-12" version="1">MSYKRKSRTYIGEARHVQRATGEFEVKLNNFNFEEMGAENTQITDISNTAPNNRSNNYDILNHLKKIIENQQDINKKIETIIGKVDRQGAELISLRNEVASIKARVCGEPNIVKLTVPRSPLASLEGFESFEDSLNNLIKDLIAINEKTFDKFIRSSWRQIISDDVAKFFSWRGTDSKKCVRGLRTTLALRHAFKQKFALDDQDFDHTTQKHFQYAQERKAGLEKSDAKKLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYJ9</accession>
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        <submittedName>
            <fullName evidence="9">Synaptic vesicle glycoprotein 2B isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="10">Synaptic vesicle glycoprotein 2B isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">LOC4803226</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001359965.4"/>
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    <dbReference type="RefSeq" id="XP_033235604.1">
        <property type="nucleotide sequence ID" value="XM_033379713.1"/>
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    <dbReference type="KEGG" id="dpo:4803226"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF36">
        <property type="entry name" value="EG:BACR7A4.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="89"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="31"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360002.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033235604.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="58411" checksum="0ED11860DBF5C2CA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZV6</accession>
    <name>A0A6I8VZV6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRIO and F-actin-binding protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236533.1">
        <property type="nucleotide sequence ID" value="XM_033380642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZV6"/>
    <dbReference type="KEGG" id="dpo:117184163"/>
    <dbReference type="InParanoid" id="A0A6I8VZV6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    <sequence length="1040" mass="115638" checksum="1DB99A1353AB4160" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1U5</accession>
    <name>A0A6I8W1U5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation-transporting ATPase</fullName>
            <ecNumber evidence="13">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 19" type="primary">anne</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18 19">MV-25-SWS-2005</strain>
            <tissue evidence="18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_033237262.1">
        <property type="nucleotide sequence ID" value="XM_033381371.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237263.1">
        <property type="nucleotide sequence ID" value="XM_033381372.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W1U5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07542">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000034">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000067">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000057">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000068">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047821">
        <property type="entry name" value="P5B-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="549"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="586"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1176"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1207"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1246"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1320"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1359"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="165"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="15">
        <location>
            <begin position="418"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="935"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1462"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="935"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033237262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033237263.1"/>
        </source>
    </evidence>
    <sequence length="1481" mass="167299" checksum="F42C9E5E971A8B52" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4D5</accession>
    <name>A0A6I8W4D5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heparan sulfate 2-O-sulfotransferase pipe-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6900249</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033237559.1">
        <property type="nucleotide sequence ID" value="XM_033381668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4D5"/>
    <dbReference type="KEGG" id="dpo:6900249"/>
    <dbReference type="InParanoid" id="A0A6I8W4D5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W4D5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF20">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE PIPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033237559.1"/>
        </source>
    </evidence>
    <sequence length="131" mass="15837" checksum="9E3BAE16DBB45E1B" modified="2020-08-12" version="1">MGFYCKEVELIERSSFSPFNSPTAVQMAKEHVERDYAVVGSWEDTNITLTVLERYIPRFFRGAKLMYEMNNNKIVNRNKNKRKPFIEPEVKAMIRRNFTNEYEFYYFCKQRLYKQYLALNLNELERHGLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4G7</accession>
    <name>A0A6I8W4G7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13 14 15 16" type="primary">Pp1-87B</name>
        <name evidence="12" type="synonym">LOC4800831</name>
    </gene>
    <organism evidence="11 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13 14">MV-25-SWS-2005</strain>
            <strain evidence="11 12">MV2-25</strain>
            <tissue evidence="12 13 14">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="RefSeq" id="XP_001358035.1">
        <property type="nucleotide sequence ID" value="XM_001357998.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038668.1">
        <property type="nucleotide sequence ID" value="XM_015183182.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038669.1">
        <property type="nucleotide sequence ID" value="XM_015183183.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238205.1">
        <property type="nucleotide sequence ID" value="XM_033382314.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238213.1">
        <property type="nucleotide sequence ID" value="XM_033382322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4G7"/>
    <dbReference type="SMR" id="A0A6I8W4G7"/>
    <dbReference type="GeneID" id="4800831"/>
    <dbReference type="KEGG" id="dpo:4800831"/>
    <dbReference type="OMA" id="RNIARPM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079030">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="10">
        <location>
            <begin position="119"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015038668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015038669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033238205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033238213.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="34553" checksum="6FCEC4FDDC843E1A" modified="2020-08-12" version="1">MADVMNIDSIISRLLEVRGARPGKNVQLSEGEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYSENFFLLRGNHECASINRIYGFYDECKRRYSIKLWKTFTDCFNCLPVAAIVDEKIFCCHGGLSPDLTSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDTMGWGENDRGVSFTFGAEVVAKFLQKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDDTLMCSFQILKPADKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W509</accession>
    <name>A0A6I8W509_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Mucin-5AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6 7 8 9" type="primary">LOC6902155</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4 5">MV-25-SWS-2005</strain>
            <tissue evidence="3 4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238415.1">
        <property type="nucleotide sequence ID" value="XM_033382524.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238416.1">
        <property type="nucleotide sequence ID" value="XM_033382525.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238417.1">
        <property type="nucleotide sequence ID" value="XM_033382526.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238418.1">
        <property type="nucleotide sequence ID" value="XM_033382527.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238419.1">
        <property type="nucleotide sequence ID" value="XM_033382528.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238420.1">
        <property type="nucleotide sequence ID" value="XM_033382529.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238422.1">
        <property type="nucleotide sequence ID" value="XM_033382531.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902155"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W509">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1025"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1152"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1218"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="700"/>
            <end position="711"/>
        </location>
    </feature>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="805"/>
            <end position="817"/>
        </location>
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        <location>
            <begin position="912"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1039"/>
        </location>
    </feature>
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        <location>
            <begin position="1040"/>
            <end position="1056"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1057"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1073"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1249"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1315"/>
            <end position="1325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033238416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033238417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033238418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033238419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033238420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033238422.1"/>
        </source>
    </evidence>
    <sequence length="1325" mass="138266" checksum="D6515699A4451C05" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W774</accession>
    <accession>Q29F26</accession>
    <name>A0A6I8W774_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Phosphatidate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">LOC4812480</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352388.3">
        <property type="nucleotide sequence ID" value="XM_001352352.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238504.1">
        <property type="nucleotide sequence ID" value="XM_033382613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W774"/>
    <dbReference type="GeneID" id="4812480"/>
    <dbReference type="KEGG" id="dpo:4812480"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071055">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF197">
        <property type="entry name" value="FI04477P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="145"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="339"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001352388.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238504.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="39786" checksum="8E6F603C14BA60F1" modified="2020-08-12" version="1">MRNANEDQSVSVSVSATASASASASASPVPEGCQSQRQAEDRQLTRRLLMELLVMVVLVVPICIYEFAVDPVRRGFFCDDESISYPFKDNTITPVMLGLISGLLPLVVLLLVEYVRHMRAGELATTVHLLGWRMSAWYVELFRQTTYFTFGLLLTFDATEVGKYTIGRLRPHFLAVCQPQLTDRTLCSDPANLHRYVENYECAAEGYTVADVRQSRLSFPSGHSSIVFYSMVYVALYLQRKLTWRSSKLSRHFLQFVVIMLAWYTALSRVMDYWHHWSDVLSGSLIGVAGALITARYIARFFDSPYESVPGILSGGLRRQNTSATLQEEVCPTTPPPYSANNSYNEDQYCSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W864</accession>
    <name>A0A6I8W864_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thioredoxin domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4815619</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239563.1">
        <property type="nucleotide sequence ID" value="XM_033383672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W864"/>
    <dbReference type="KEGG" id="dpo:4815619"/>
    <dbReference type="InParanoid" id="A0A6I8W864"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031827">
        <property type="entry name" value="DUF4746"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051766">
        <property type="entry name" value="TXND_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46135">
        <property type="entry name" value="NME/NM23 FAMILY MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46135:SF3">
        <property type="entry name" value="NME_NM23 FAMILY MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15928">
        <property type="entry name" value="DUF4746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="15"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4746" evidence="3">
        <location>
            <begin position="233"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239563.1"/>
        </source>
    </evidence>
    <sequence length="587" mass="65708" checksum="1E41743960346759" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WA98</accession>
    <name>A0A6I8WA98_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Usp10</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family. USP10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="RefSeq" id="XP_033240325.1">
        <property type="nucleotide sequence ID" value="XM_033384434.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8WA98">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812931"/>
    <dbReference type="InParanoid" id="A0A6I8WA98"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WA98">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030330">
        <property type="term" value="P:DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000092">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF687">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="1266"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="181"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
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            <begin position="1137"/>
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            <dbReference type="ARBA" id="ARBA00000707"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005427"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033240325.1"/>
        </source>
    </evidence>
    <sequence length="1672" mass="182222" checksum="1831E1F6E7C9213C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAM4</accession>
    <accession>Q29G96</accession>
    <name>A0A6I8WAM4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Ras-related protein Rab-39B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">Rab39</name>
        <name evidence="14" type="synonym">LOC4814813</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15">MV-25-SWS-2005</strain>
            <strain evidence="14">MV2-25</strain>
            <tissue evidence="14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
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        <property type="nucleotide sequence ID" value="XM_033384431.1"/>
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    <dbReference type="KEGG" id="dpo:4814813"/>
    <dbReference type="OMA" id="LGEQCPC"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071493">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04111">
        <property type="entry name" value="Rab39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAB39B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
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    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="RAB"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033240322.1"/>
        </source>
    </evidence>
    <sequence length="218" mass="24590" checksum="2DC6F6046E3E5E3A" modified="2020-08-12" version="1">MVEPIFEYQFRLILIGDSTVGKSSLLKFFTDGKFAELSDPTVGVDFFARLIEMKDGTQIKLQLWDTAGQERFRSITKSYYRNSVGVLLVYDISNHASFEHIPLWMMEAQRHIEPHRPVFALVGCKLDLINAGGHREVSTEEAQTFAKQHGLHFVETSARTGANVEEAFRMVTQEVYARIRSGEYKAEDGWDGIKAGFATPNSLDFNLVVAEPEKSSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCL2</accession>
    <name>A0A6I8WCL2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4780 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">LOC26534114</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
            <tissue evidence="4 5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240298.1">
        <property type="nucleotide sequence ID" value="XM_033384407.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240299.1">
        <property type="nucleotide sequence ID" value="XM_033384408.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033384409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCL2"/>
    <dbReference type="KEGG" id="dpo:26534114"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WCL2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031961">
        <property type="entry name" value="DUF4780"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16012">
        <property type="entry name" value="DUF4780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4780" evidence="2">
        <location>
            <begin position="116"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033240298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033240299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033240300.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="40408" checksum="B312155392B835DD" modified="2020-08-12" version="1">MKAKAQYKAALNIKSRLQGKADISQEEKVKLAWAEQRVEEGRLHYAQMPQMRASNGEFPNMVEEMMATKRQRSTESAIKDTPASKRQRGTKSAAPPPKAAKKPTPQLKAKVSDVTKRHLIVALIDRSDENGRMTAAQWKLVHARLVNALFARMEEDPADPMPTFDGAGWLNGVKILKCKDVPTLRWLTRTVCQLDAMWEGAKLEVVDRELVPSKPKAKVLFPIKIQGDRALKLLQCQNADVPTADWRILYICSPLPSEGGQCVILQINKEAEDLLYPRFGKMAWGMGSVYLRLKKRHPKNAHSLLAGEVEKDLGLESIVVATQGLGLEDSEEEEEDGDLTLDVNPSCATEPATYDAECAAAQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDF7</accession>
    <name>A0A6I8WDF7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4813664</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033241423.1">
        <property type="nucleotide sequence ID" value="XM_033385532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDF7"/>
    <dbReference type="InParanoid" id="A0A6I8WDF7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF340">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="72"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033241423.1"/>
        </source>
    </evidence>
    <sequence length="493" mass="53800" checksum="4E55A87525AA9FDF" modified="2020-08-12" version="1">MPSSQQKQQHQQQKKVRGRRHRLAIRMDPELKRNLVPFHVRALGVAQRSMGSPLVYEAQVLRPDVENTMTDLETFLNLVKCAFGTGCLAMPRAFYNAGWLIGLLATVLIGFIVVYAMHVLLNDIQHLCRRHRMAVLSYRETMELALLDGPTWLHCMSRPLGFVQSGPHYIPSVFIPILQLLRGHSDVCLSFRCGLCLHCVYSQEPKILGGSVSLSHGSASVYGPADPPTDTHVPREESEVSVAVYCHLQYPDCRQFWHHFLVSCAGFAQLGGTAGHTALDAVPSLLWHCSVCHRISRCYFSSAAQHAASRELSGLLRGPQSGHGPRGPLLCSVWVLWLLAIREGHGQLHSAQPAAPRNPATVRDGNVCDGHVLQLRLAGLRHRGHHLAGIHAAQAGGECCLRAIRGVSGAPCPRDCLCAGGHWLSGLWAAPLLCGILLPGTIGAHLSGNRQHVCAVQPGLWLWQDPALAVPLLPRVGPLGGDLWYSDLGEGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEA0</accession>
    <name>A0A6I8WEA0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Lateral signaling target protein 2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4802219</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359186.2">
        <property type="nucleotide sequence ID" value="XM_001359149.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241552.1">
        <property type="nucleotide sequence ID" value="XM_033385661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEA0"/>
    <dbReference type="KEGG" id="dpo:4802219"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359186.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241552.1"/>
        </source>
    </evidence>
    <sequence length="624" mass="68649" checksum="D6F01C6E0DF2B850" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEL1</accession>
    <name>A0A6I8WEL1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transcription factor Ouib-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC117185673</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241861.1">
        <property type="nucleotide sequence ID" value="XM_033385970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEL1"/>
    <dbReference type="FunCoup" id="A0A6I8WEL1">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117185673"/>
    <dbReference type="InParanoid" id="A0A6I8WEL1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WEL1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000093">
        <property type="entry name" value="zinc finger protein 668 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="14">
        <location>
            <begin position="3"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="337"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033241861.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="44107" checksum="2D821D3D9799EB2E" modified="2020-08-12" version="1">MALLCRTCGEHAVNPRKLFDENGNDILKDILKLTGVWLTNRRGFPSHICASCLQQTNEAMGFRELCIRTNKFWYTTADSKTTDAVHTDPLMGNEGAAIPKENKEYVKVEIETLYEEIQPASPLSPHGSSNRQSEQYNIANDREVFDNVLDLDRLINEEDAAIVVEEENNEEMNDIPSPLSSPKSRLDSCSVTTKKVLRGSRKSNVDPLPKKKRYRSTRGFYCDQCGKWFKDKCNLNVHLKRHTGVKQFECEECGRKELTMHLLSLHIRVKHKGELPYSCKYCGQRFDNCIKRLNHERRKHKECPDTRPHVCPVCGKAFTRKAELKRHEIVHTGEQPFHCETCDVYFNQNSSLKTHNRSKLHIKKVGQDRTRIQIDVFTDEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WF34</accession>
    <name>A0A6I8WF34_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein X11L isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">X11L</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241509.1">
        <property type="nucleotide sequence ID" value="XM_033385618.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241510.1">
        <property type="nucleotide sequence ID" value="XM_033385619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WF34"/>
    <dbReference type="KEGG" id="dpo:4814789"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WF34">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06720">
        <property type="entry name" value="PDZ1_APBA1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06793">
        <property type="entry name" value="PDZ2_APBA1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01208">
        <property type="entry name" value="PTB_X11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000007">
        <property type="entry name" value="Amyloid beta A4 protein-binding family A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000017">
        <property type="entry name" value="Amyloid beta A4 protein-binding family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051230">
        <property type="entry name" value="APP-Binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345">
        <property type="entry name" value="SYNTENIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345:SF16">
        <property type="entry name" value="X11L, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PID" evidence="5">
        <location>
            <begin position="802"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="1024"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="1115"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="457"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="759"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="902"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="458"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="774"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="914"/>
            <end position="923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033241509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033241510.1"/>
        </source>
    </evidence>
    <sequence length="1205" mass="130439" checksum="102F0D6F00ECC8B4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WF71</accession>
    <name>A0A6I8WF71_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">oxaloacetate tautomerase</fullName>
            <ecNumber evidence="5">5.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Fumarylacetoacetate hydrolase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6897520</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>oxaloacetate = enol-oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:16021"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17479"/>
            <dbReference type="EC" id="5.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="4">
            <dbReference type="Rhea" id="RHEA:16023"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EC" id="5.3.2.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_033241694.1">
        <property type="nucleotide sequence ID" value="XM_033385803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WF71"/>
    <dbReference type="FunCoup" id="A0A6I8WF71">
        <property type="interactions" value="1647"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897520"/>
    <dbReference type="InParanoid" id="A0A6I8WF71"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WF71">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000017">
        <property type="entry name" value="Acylpyruvase FAHD1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="6">
        <location>
            <begin position="26"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00042340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033241694.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25197" checksum="9777B6E8F3206909" modified="2020-08-12" version="1">MVRLSSRFLMAVCNQDTANFIRDGKKIVGVALNYMDVVKARNVPVPKEPLIFLKPTSSYLREGRPIILPKVFSKVAYEVELGVVIGARCKNVSKENAMKYVSGYCLALDLTAQCNLAAARAAGHPWTLGKGFDTSTPVSRFIPFDDVDDPHTLPLWLKVNGQLKQQGCTADLIFKVPDIISYVSKYMTLEPNDLILTGTPNGSDAFKAGDEIECGMAEFARLKFQVCGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>B5DM17</accession>
    <accession>A0A6I8UZ72</accession>
    <name>B5DM17_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein 192</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6901205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133906.2">
        <property type="nucleotide sequence ID" value="XM_002133870.3"/>
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    <dbReference type="GeneID" id="6901205"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
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        <location>
            <begin position="1"/>
            <end position="24"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133906.2"/>
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    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="216" mass="25095" checksum="59D60049361B7926" modified="2016-01-20" version="2">MCCISTHKLKLLVVCLTLLVQIDGELKAANNATLLLYYSRTKFLGYLITRIILGIFLAIWAFTGFLSLFYDSVWGVATYSSGICFLTVLDLFTLYYRYVLRNQVYTDENERMKLERRYKWQGGQHNHETLDEGIGDAMLLVINIVQVAIQVSVNIAGWWLAVRLRRHHQNEEKYEADKVYLEYLLKTHPHTTPPSNAQSQHPQQQHQNSKRPSVMK</sequence>
</entry>
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    <accession>B5DM77</accession>
    <accession>A0A6I8UZ53</accession>
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        <name evidence="10" type="primary">LOC6901266</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <text evidence="2">Belongs to the TMEM120 family.</text>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 120 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
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    </dbReference>
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        <property type="entry name" value="TMEM120A-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="156"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="351"/>
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            <dbReference type="ARBA" id="ARBA00004141"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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            <dbReference type="RefSeq" id="XP_002133967.1"/>
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    <sequence length="386" mass="45574" checksum="21616CC2A6698CCD" modified="2008-10-14" version="1">MDIDSLKNEWEELNKEYTELENCNRRYIELLEQLHSRQQICFNEIKHQRYRMNQITASLRQFKGPVDVEVKEKVDELQKMTLKRKAQLHEIEQSLPAKSGRYLQIILGDVNVSILNRNDKVRYKDDYEKFKLILNVIGLIMAFFNLIFNYRALELAFIFLLVWYYCTLTIRESILKVNGSRIKGWWRAHHFISTVAAGVLLVWPQGEHWQIFRMQFMYFNVYISIVQYLQFGYQKGLLYRLKALGERHNMDITIEGFHSWMWRGLSFLLPFLFIGYWFQAYNAWTLYKLAFSPPDAPWHVSVMSGLFLLLFIGNMATTLWVVPEKIRARSKERYRLLSMGKSMKLRREVKNSASDMDLSSSANGSTPKTATGSLTEQQPPADKKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>B5DNI0</accession>
    <accession>A0A6I8UZU6</accession>
    <name>B5DNI0_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 3 subunit A</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC6901691</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134361.2">
        <property type="nucleotide sequence ID" value="XM_002134325.3"/>
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    <dbReference type="STRING" id="46245.B5DNI0"/>
    <dbReference type="GeneID" id="6901691"/>
    <dbReference type="KEGG" id="dpo:6901691"/>
    <dbReference type="eggNOG" id="KOG0907">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="InParanoid" id="B5DNI0"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244863">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DNI0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02948">
        <property type="entry name" value="TRX_NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031827">
        <property type="entry name" value="DUF4746"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051766">
        <property type="entry name" value="TXND_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46135">
        <property type="entry name" value="NME/NM23 FAMILY MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46135:SF3">
        <property type="entry name" value="NME_NM23 FAMILY MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15928">
        <property type="entry name" value="DUF4746"/>
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    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
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        <property type="entry name" value="THIOREDOXIN_1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="550"/>
            <end position="748"/>
        </location>
    </feature>
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        <location>
            <begin position="550"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="585"/>
        </location>
    </feature>
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        <location>
            <begin position="586"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="604"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="709"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134361.2"/>
        </source>
    </evidence>
    <sequence length="748" mass="81268" checksum="69E7D0899CAF1F24" modified="2016-01-20" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-03-02" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q28X71</accession>
    <name>UFC1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Ubiquitin-fold modifier-conjugating enzyme 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Ufm1-conjugating enzyme 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GA21037</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL26445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q28X71"/>
    <dbReference type="SMR" id="Q28X71"/>
    <dbReference type="FunCoup" id="Q28X71">
        <property type="interactions" value="1567"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q28X71"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0277451">
        <property type="protein sequence ID" value="FBpp0275889"/>
        <property type="gene ID" value="FBgn0081025"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805471"/>
    <dbReference type="eggNOG" id="KOG3357">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101170_0_0_1"/>
    <dbReference type="InParanoid" id="Q28X71"/>
    <dbReference type="OMA" id="LWQKNVP"/>
    <dbReference type="PhylomeDB" id="Q28X71"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081025">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061657">
        <property type="term" value="F:UFM1 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11686">
        <property type="entry name" value="UBCc_UFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000042">
        <property type="entry name" value="Ubiquitin-fold modifier-conjugating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014806">
        <property type="entry name" value="Ufc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12921">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER-CONJUGATING ENZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12921:SF0">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER-CONJUGATING ENZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08694">
        <property type="entry name" value="UFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008716">
        <property type="entry name" value="DUF1782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="chain" id="PRO_0000391973" description="Ubiquitin-fold modifier-conjugating enzyme 1">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="164" mass="19001" checksum="0FE6C14C524C1877" modified="2006-04-04" version="1">MVDDSTRKTLSNIPLLQIRAGPREKDVWVQRLKEEYHALIMYVKNNKQSGSDWFRLESNKEGTKWFGKCWYMHNLLKYEFDVEFDIPVTYPTTAPEIALPELDGKTAKMYRGGKICLTDHFKPLWARNVPKFGIAHAMALGLAPWLAVEVPDLIEKGIITYKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E50</accession>
    <accession>A0A6I8UB52</accession>
    <name>Q29E50_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tektin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Tektin-C</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme. Forms filamentous polymers in the walls of ciliary and flagellar microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
            <location evidence="10">Cytoskeleton</location>
            <location evidence="10">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the tektin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352713.1">
        <property type="nucleotide sequence ID" value="XM_001352677.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E50"/>
    <dbReference type="SMR" id="Q29E50"/>
    <dbReference type="FunCoup" id="Q29E50">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E50"/>
    <dbReference type="GeneID" id="4811967"/>
    <dbReference type="KEGG" id="dpo:4811967"/>
    <dbReference type="eggNOG" id="KOG2685">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033588_2_2_1"/>
    <dbReference type="InParanoid" id="Q29E50"/>
    <dbReference type="OMA" id="LAMVMDE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070442">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29E50">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF25">
        <property type="entry name" value="TEKTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 10" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001352713.1"/>
        </source>
    </evidence>
    <sequence length="421" mass="49110" checksum="DE802671B265A88C" modified="2006-04-04" version="1">MLPNLKARRITGMQLAGKHLGPVAKCPPRYSEEDWDYNNKIKFRITCDQEKLAERIVEESRRVVDETKDTTKNWQREVEHHMRERTSEIRFLVDELNRQKKTAMLEDEALNTYRNRVLNAIEFLKEKSLAICKQCLILREGRIGVDLCDDEVDRSLRRELKVIKGCQGLADAALKEAEEQIRKLRAAIYLLDQDLAAKDKSLAIDEKNLKLKEFQHDLGKGQDLSKSHCQFSLTEWQAQTYENLEANAKALVSAGQLRAYIDLLLKQVCEDMQNQTDRTNEAFERRIAETKHVKQCLENKHKDTMDHIHQVQRNMTDLEKEMMDKQRAITLCQTRLSNRAHRPGLELTCDMVQDALYNELQALKASVCKLNQKLNENKASMRYLMHVQVMQEEEINIKANTCKIDEVDCMTLRQALKYQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29ER8</accession>
    <accession>A0A6I8UAP4</accession>
    <name>Q29ER8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Disco-interacting protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">DIP2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DIP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352495.1">
        <property type="nucleotide sequence ID" value="XM_001352459.4"/>
    </dbReference>
    <dbReference type="SMR" id="Q29ER8"/>
    <dbReference type="FunCoup" id="Q29ER8">
        <property type="interactions" value="743"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29ER8"/>
    <dbReference type="GeneID" id="4812044"/>
    <dbReference type="KEGG" id="dpo:4812044"/>
    <dbReference type="eggNOG" id="KOG3628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001345_0_0_1"/>
    <dbReference type="InParanoid" id="Q29ER8"/>
    <dbReference type="OMA" id="CERPQVA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080033">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29ER8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd05905">
        <property type="entry name" value="Dip2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000001">
        <property type="entry name" value="DIP2 disco-interacting protein 2 homolog C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037337">
        <property type="entry name" value="Dip2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010506">
        <property type="entry name" value="DMAP1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754:SF32">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23024">
        <property type="entry name" value="AMP-dom_DIP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06464">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01137">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51912">
        <property type="entry name" value="DMAP1_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352495.1"/>
        </source>
    </evidence>
    <sequence length="1793" mass="195704" checksum="87E38BEDB9D34935" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HM9</accession>
    <accession>A0A6I8UFM4</accession>
    <name>Q29HM9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SMC3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354674.2">
        <property type="nucleotide sequence ID" value="XM_001354638.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HM9"/>
    <dbReference type="SMR" id="Q29HM9"/>
    <dbReference type="FunCoup" id="Q29HM9">
        <property type="interactions" value="2205"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HM9"/>
    <dbReference type="GeneID" id="4814811"/>
    <dbReference type="KEGG" id="dpo:4814811"/>
    <dbReference type="eggNOG" id="KOG0964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_5_0_1"/>
    <dbReference type="InParanoid" id="Q29HM9"/>
    <dbReference type="OMA" id="GQKTVCA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082030">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03272">
        <property type="entry name" value="ABC_SMC3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1060.20:FF:000002">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1620:FF:000002">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000370">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000424">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041741">
        <property type="entry name" value="SMC3_ABC_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0469">Meiosis</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001354674.2"/>
        </source>
    </evidence>
    <sequence length="1200" mass="140131" checksum="090E7E992FA0931F" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NS4</accession>
    <accession>A0A6I8ULM5</accession>
    <name>Q29NS4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transcription factor E2F2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">E2f2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357500.1">
        <property type="nucleotide sequence ID" value="XM_001357464.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NS4"/>
    <dbReference type="SMR" id="Q29NS4"/>
    <dbReference type="FunCoup" id="Q29NS4">
        <property type="interactions" value="1608"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NS4"/>
    <dbReference type="GeneID" id="4818188"/>
    <dbReference type="KEGG" id="dpo:4818188"/>
    <dbReference type="eggNOG" id="KOG2577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_750673_0_0_1"/>
    <dbReference type="InParanoid" id="Q29NS4"/>
    <dbReference type="OMA" id="FQNYMAG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070569">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000458">
        <property type="entry name" value="E2F-like (Mammalian transcription factor)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015633">
        <property type="entry name" value="E2F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081:SF18">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="9">
        <location>
            <begin position="77"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="246"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357500.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="42305" checksum="E9ED364A01D52FF2" modified="2006-04-04" version="1">MYKRKSSSIVLRDSAGPGSALKMKNNNAKGSVSNVDTDEAMTIKAYENETVQMDMSQDHQEQQPLKSPSQSHPVQQRSVGSLVLLTQKFVQLMKSNGGSIDLKEATKILDVQKRRIYDITNVLEGIGLIDKGRHCSLVRWRGGGFNNAKDQEEYDVAREGTNNLKKEEEDLDMQLEYAQRNLRYVMQDPTNLSYAYLTRDDLLQIYADDSVFTIPNYDEEVEIQRSDNELRVSLDNGSTIDIRLVTNQGKSTTNPNDADGFFDYRRLDTPSPSTSSRSSEDGTGPTSTGNVITDEHTYSCNPDLKEEMKLLENELTAKIIFQNYMTGHSLRRFYPDDPNLENPPLVQLNPPHDDFNFVLKSDEGICELFDVQCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29P30</accession>
    <accession>A0A6I8ULE3</accession>
    <name>Q29P30_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DnaJ homolog subfamily C member 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4817832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357394.1">
        <property type="nucleotide sequence ID" value="XM_001357358.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29P30"/>
    <dbReference type="FunCoup" id="Q29P30">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29P30"/>
    <dbReference type="GeneID" id="4817832"/>
    <dbReference type="KEGG" id="dpo:4817832"/>
    <dbReference type="eggNOG" id="KOG0568">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040968_0_0_1"/>
    <dbReference type="InParanoid" id="Q29P30"/>
    <dbReference type="OMA" id="EWISLGK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072563">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29P30">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000182">
        <property type="entry name" value="dnaJ homolog subfamily C member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052573">
        <property type="entry name" value="DnaJ_C_subfamily_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39158:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39158">
        <property type="entry name" value="OS08G0560600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DJC28_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001357394.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="41993" checksum="4F8200BCB5C97C35" modified="2006-04-04" version="1">MLKAGKLFGSLIGSAGGRIALREVHLKRREVYLQCFRILGVCEGADQNTVRQAYLDLVKRVHPDSGCDEASAERFQQVDEAFKLLQEKFAKGRRNIQEDEEQAMEFDIKHTAPQHRQYLSNEGIGVGTPFQRQKQYQQVRAMKAQERVLEHRIDKAAAGDSDLISKEGNHFQKHAIKTKYGIDRVVEDLIQEAMSKGDFNNLNGSGKPLSSAQSQNPYLDFTTHKLNKIMLDNGFTPEWITLGKEIRDARMQLLKTVRQERVYYGDWPLQNAEEQAAWLRFTQDHEEEVQELNKMIDKYNLIVPILKNQFFRYRLDRVAAMVFKEPDLKRNQKRPAVQYKAKDEQSENTFKSNIFDLITRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LYK0</accession>
    <accession>A0A6I8UEB9</accession>
    <name>Q2LYK0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 104 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4813964</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TMEM104 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354123.1">
        <property type="nucleotide sequence ID" value="XM_001354087.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LYK0"/>
    <dbReference type="FunCoup" id="Q2LYK0">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LYK0"/>
    <dbReference type="GeneID" id="4813964"/>
    <dbReference type="KEGG" id="dpo:4813964"/>
    <dbReference type="eggNOG" id="KOG3832">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025541_1_0_1"/>
    <dbReference type="InParanoid" id="Q2LYK0"/>
    <dbReference type="OMA" id="GHREGHP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078769">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LYK0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 104-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="137"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354123.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="57158" checksum="206917AB3B49F30A" modified="2006-02-21" version="1">MPRLISRPEPAPTYSNLVGFIFIFNLIVGTGALTLPGVFAKAGWVLSLIVIILLATISYMTVTFIIEAMACANAIKNWQTLQALRHHSRSSAESSENDDASADDQGSDLRDLERVPLNIQNAENHYYQLSHKFELGEMATIFFNEFGRIMFFLCLIVYLYGDLSIYSAAVSRSLRDVICDQTNATDSPNSRLMFWPGDFRNNTAHACWKEHTISRLSVYRVILIGFTLIFGPFAAFSIQKTKYLQILTAIFRWMAFTLMICISLKLLISRGPRGHPVSFNVYGIPSLFGACVYSFMCHHSLPSLLAPLKHKLMVSKILSFDYIIICAFYIVLAMTGIFAFDRIEDLYTLNFLPYDVDYTDFASGLLIGIDYFLALFPIFTLSASFPIIAVTLKNNLQTLFLDMSRYDTYSPFVRILFPLCAIIPPFCVTYFTESLASLVAFTGSYAGTGIQYIIPVFLVYFARRTCSELLGSGIVNSFESPFKSSAWLIFVIIWSILCVCLVTVNMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M060</accession>
    <accession>A0A6I8UDB0</accession>
    <name>Q2M060_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 134</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4813721</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM134/TMEM230 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353563.1">
        <property type="nucleotide sequence ID" value="XM_001353527.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M060"/>
    <dbReference type="FunCoup" id="Q2M060">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M060"/>
    <dbReference type="GeneID" id="4813721"/>
    <dbReference type="KEGG" id="dpo:4813721"/>
    <dbReference type="eggNOG" id="KOG4753">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1505047_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M060"/>
    <dbReference type="OMA" id="GFICFIP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071401">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039714">
        <property type="entry name" value="TMEM134"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008590">
        <property type="entry name" value="TMEM_230/134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13558">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13558:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05915">
        <property type="entry name" value="TMEM_230_134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353563.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="19257" checksum="09A9B1B25162A530" modified="2006-02-21" version="1">MYNNGARGFSIHDAFEEETDEAIRVYGSTVISTPMRGGKQGRSTEDVSIRIQDPKGYNKYAEDTTSRDSDSLIQEYGNLPTEETNTYCWRHPKVRENWRTVLAAFTLLIVGTGLFVMGTFAIADPQNTSQGVVFFVAGLICFIPGAYHVVYIWLAAKGYRGFDFYHLPLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0D4</accession>
    <accession>A0A6I8UCT4</accession>
    <name>Q2M0D4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like-conjugating enzyme ATG3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Autophagy-related protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Atg3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353489.1">
        <property type="nucleotide sequence ID" value="XM_001353453.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0D4"/>
    <dbReference type="FunCoup" id="Q2M0D4">
        <property type="interactions" value="2842"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0D4"/>
    <dbReference type="GeneID" id="4813707"/>
    <dbReference type="KEGG" id="dpo:4813707"/>
    <dbReference type="eggNOG" id="KOG2981">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027518_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M0D4"/>
    <dbReference type="OMA" id="HCPTWSW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079921">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0D4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.50:FF:000001">
        <property type="entry name" value="Autophagy-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001353489.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="36875" checksum="291909349F8AAA69" modified="2006-02-21" version="1">MQSVLNSVKGTALNVAEYLTPVLKESKFRETGVLTPEEFVAAGDHLVHHCPTWQWAAGDDTKTKPYLPKDKQFLITRNVPCYRRCKQMEYVGEETLVEEESGDGGWVETHQLNDDGTTQLEEKICELTMEETKDEMHTPDSDKSAPGDAVDGEDEDDDEAIDMDAFEESGMLDLVDPAVATTTRKLEAEPKSSPAAAATSEASGDSVLHTRTYDLHITYDKYYQTPRLWVVGYDEQRKPLTVEQMYEDVSQDHAKKTVTMESHPHLPGPNMASVHPCRHADIMKKIIQTVEEGGGQLGVHLYLIIFLKFVQTVIPTIEYDFTQNFNMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NUF6</accession>
    <accession>A0A6I8V9L0</accession>
    <name>A0A0R3NUF6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26534258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036673.1">
        <property type="nucleotide sequence ID" value="XM_015181187.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NUF6"/>
    <dbReference type="GeneID" id="26534258"/>
    <dbReference type="KEGG" id="dpo:26534258"/>
    <dbReference type="InParanoid" id="A0A0R3NUF6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0273245">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015036673.1"/>
        </source>
    </evidence>
    <sequence length="70" mass="8062" checksum="C790C8B2CD74F916" modified="2016-01-20" version="1">MSDKKASSRATPPGMQFSTPVGAPETRRKMLLFKRLLRRELQREAQTPQQIRTARQRRDQQEAHGVFPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NY21</accession>
    <accession>A0A6I8WB93</accession>
    <name>A0A0R3NY21_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117185049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240547.1">
        <property type="nucleotide sequence ID" value="XM_033384656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NY21"/>
    <dbReference type="KEGG" id="dpo:117185049"/>
    <dbReference type="KEGG" id="dpo:26532270"/>
    <dbReference type="InParanoid" id="A0A0R3NY21"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271257">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NY21">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240547.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16831" checksum="1B2BB2D3D2468E04" modified="2016-01-20" version="1">MEMAESMKEAGLELGWGEIRTKLENMTKKYRIEKNKIGPSGGAPSGWQHYDKLNSFLGCCRINNMEQSTLDNYNPNKYFSDIIEMDLDGSCCSSTLSGPPPKKKKKTDISLLAEIAQERLEQQKDHHVLLCNHSRNNCYIFIIHYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P196</accession>
    <accession>A0A6I8W8I3</accession>
    <name>A0A0R3P196_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4815373</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues, with a preference for phosphotyrosine as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="RefSeq" id="XP_033239685.1">
        <property type="nucleotide sequence ID" value="XM_033383794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P196"/>
    <dbReference type="SMR" id="A0A0R3P196"/>
    <dbReference type="FunCoup" id="A0A0R3P196">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P196"/>
    <dbReference type="KEGG" id="dpo:4815373"/>
    <dbReference type="InParanoid" id="A0A0R3P196"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080302">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P196">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14515">
        <property type="entry name" value="DUSP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF5">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
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    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="70"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="150"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="6">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR620405-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033239685.1"/>
        </source>
    </evidence>
    <sequence length="235" mass="26879" checksum="A4E461F0908EE607" modified="2016-01-20" version="1">MSWRYALSTDRYSSSIGDRYSPHYYQSPSRLETSEQTTGRQLQRVLHYSMAPTRALPGLRRAECAIHDVDCDEVYPGIYIGDAAAAKNKTYLRMMGITHVLNAAEGCRYGQVDTGHSYYRDMPSIRRNHKDCVFRYMGFPMIDAPTTDISRYFYVASKFIDSAISSGGKILVHCLVGMSRSATCVLAYLMICRKMSAVDAIRTVRMRRDIRPNDGFLQQLADLDMELKRKNLYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAM9</accession>
    <name>A0A6I8UAM9_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Homeotic protein empty spiracles</fullName>
        </submittedName>
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        <name evidence="10" type="primary">exex</name>
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    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ephydroidea</taxon>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="10">MV-25-SWS-2005</strain>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="HD"/>
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            <end position="543"/>
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            <dbReference type="RefSeq" id="XP_001352515.3"/>
        </source>
    </evidence>
    <sequence length="575" mass="61494" checksum="9EDDE44DEC2908DD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF38</accession>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <end position="47"/>
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            <end position="110"/>
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    <sequence length="137" mass="14481" checksum="2F31782E8445F78B" modified="2020-08-12" version="1">MELPRLTSGKSSRSKGHQKNTDAEQKQTEKDAAQKTTTEEAASTSTAVPSITPARIPTSEDTIALASAVVATLPGTPLVPSQTSNERRDSNVVLPVAPVPPAQTPQPTPAPESTDGDDTEEAASERRGIIHRLFGWN</sequence>
</entry>
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        <property type="entry name" value="Zig-8"/>
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        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 14, ISOFORM A-RELATED"/>
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        <property type="entry name" value="IGc2"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 6" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634432" evidence="2">
        <location>
            <begin position="29"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="84"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="184"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354529.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015041434.2"/>
        </source>
    </evidence>
    <sequence length="334" mass="37699" checksum="CE8EF3DF0F63348E" modified="2020-08-12" version="1">MCISCVMRSRLFWIFAIIYSSLHHIGSGSTSTTLKRPRAGDPFDTFPKNFWQEFSSPFTDTPEDEELEVTETTTHEPFPFFADPYTTVNISTQLSSNVYLHCRVNDLQGKTVSWMRRRGDDLTLITFGQHTYSGDSRYSLEFEEPNDWKLLIQFANERDEGPYECQVSSHPPLVLLVYLTIIVPHVEILDERGSATPEKYYKAGSTIELQCVISKIPHPSSYITWRHGPRLLNYDTSRGGISVKTDMLPGRALSRLYIANANRQDTGNYTCMLGNEITETVVVHVLNGEEPAAMQHANGTRHGARPSNMVVFFLLYVYACVCGMAVAVGHPIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFL5</accession>
    <name>A0A6I8UFL5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein Gcna</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Gcna</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354739.2">
        <property type="nucleotide sequence ID" value="XM_001354703.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFL5"/>
    <dbReference type="FunCoup" id="A0A6I8UFL5">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814815"/>
    <dbReference type="InParanoid" id="A0A6I8UFL5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFL5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006640">
        <property type="entry name" value="SprT-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035240">
        <property type="entry name" value="SprT_Zn_ribbon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23099:SF0">
        <property type="entry name" value="GERM CELL NUCLEAR ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23099">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10263">
        <property type="entry name" value="SprT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17283">
        <property type="entry name" value="Zn_ribbon_SprT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00731">
        <property type="entry name" value="SprT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SprT-like" evidence="2">
        <location>
            <begin position="829"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354739.2"/>
        </source>
    </evidence>
    <sequence length="1053" mass="117643" checksum="BB90F9A3F2C22DA9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFW0</accession>
    <name>A0A6I8UFW0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amyloid protein-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Pat1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354835.4">
        <property type="nucleotide sequence ID" value="XM_001354799.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFW0"/>
    <dbReference type="FunCoup" id="A0A6I8UFW0">
        <property type="interactions" value="1344"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814586"/>
    <dbReference type="InParanoid" id="A0A6I8UFW0"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042476">
        <property type="entry name" value="APPBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46575">
        <property type="entry name" value="AMYLOID PROTEIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46575:SF1">
        <property type="entry name" value="AMYLOID PROTEIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="547"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354835.4"/>
        </source>
    </evidence>
    <sequence length="713" mass="81349" checksum="0320853FE7BCFA84" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFW1</accession>
    <name>A0A6I8UFW1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">mRNA-capping enzyme</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">mRNA 5'-triphosphate monophosphatase</fullName>
                <ecNumber evidence="10">3.6.1.74</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">mRNA 5'-phosphatase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">mRNA guanylyltransferase</fullName>
                <ecNumber evidence="10">2.7.7.50</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">GTP--RNA guanylyltransferase</fullName>
                <shortName evidence="10">GTase</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="17" type="primary">mRNA-cap</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the C-terminal section; belongs to the eukaryotic GTase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.74" evidence="10"/>
    <dbReference type="EC" id="2.7.7.50" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001354825.3">
        <property type="nucleotide sequence ID" value="XM_001354789.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFW1"/>
    <dbReference type="FunCoup" id="A0A6I8UFW1">
        <property type="interactions" value="2179"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814475"/>
    <dbReference type="InParanoid" id="A0A6I8UFW1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFW1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17664">
        <property type="entry name" value="Mce1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000291">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000040">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000040">
        <property type="entry name" value="mRNA-capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017074">
        <property type="entry name" value="mRNA_cap_enz_bifunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036958">
        <property type="entry name" value="mRNA_capping_HCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="2 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 10 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="114"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="192"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="623"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="635"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="11">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" description="N6-GMP-lysine intermediate" evidence="12">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="389"/>
            <end position="391"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="506"/>
            <end position="508"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="578"/>
            <end position="583"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036958-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036958-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001354825.3"/>
        </source>
    </evidence>
    <sequence length="655" mass="74903" checksum="B98FE55006F10BAE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGK5</accession>
    <name>A0A6I8UGK5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">sn-1-specific diacylglycerol lipase ABHD11</fullName>
            <ecNumber evidence="3">3.1.1.116</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Alpha/beta hydrolase domain-containing protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4814584</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1,2-didecanoylglycerol + H2O = decanoylglycerol + decanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48596"/>
            <dbReference type="ChEBI" id="CHEBI:11152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27689"/>
            <dbReference type="ChEBI" id="CHEBI:90605"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol + H2O = 2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77107"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:77129"/>
            <dbReference type="ChEBI" id="CHEBI:186738"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + H2O = 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
            <dbReference type="ChEBI" id="CHEBI:75728"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77103"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
            <dbReference type="ChEBI" id="CHEBI:75468"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.116"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,3-diacyl-sn-glycerol + H2O = a 1-acyl-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38503"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:64683"/>
            <dbReference type="ChEBI" id="CHEBI:77272"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.116" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001355143.3">
        <property type="nucleotide sequence ID" value="XM_001355107.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGK5"/>
    <dbReference type="FunCoup" id="A0A6I8UGK5">
        <property type="interactions" value="1474"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814584"/>
    <dbReference type="InParanoid" id="A0A6I8UGK5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGK5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="12">
        <location>
            <begin position="61"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355143.3"/>
        </source>
    </evidence>
    <sequence length="319" mass="36359" checksum="3E1D46C777F3478A" modified="2020-08-12" version="1">MLLAKTLRFLQICRFNAKCPPTTAWATAPLCGATQRLYASSPIDMSFELFEGQTSDSSQAPLITMHGLFGSKQNWRGISKALAQRTNRKIYTVDARNHGESPHTTTHNSPSMSNDVRRFLEMRSYTKACLMGHSMGGRSMMLFAHMYPEMTERLIVVDISPIRVPRSTGEMQRIFDAMINLSLSPDLPLSEGRKLAQQQLLKATDSSETVQFIMLNLRKDPQTGAFSWACNAKLLNEFLPRFHDYRSHFASLPPYRGPTTFICGTQSPYMKREDWPQILEMFPNAEIHWLNAHHLVHFEQPQQFISIVTEFLNRPLSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGX7</accession>
    <name>A0A6I8UGX7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4815931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355307.3">
        <property type="nucleotide sequence ID" value="XM_001355271.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGX7"/>
    <dbReference type="KEGG" id="dpo:4815931"/>
    <dbReference type="InParanoid" id="A0A6I8UGX7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355307.3"/>
        </source>
    </evidence>
    <sequence length="118" mass="12683" checksum="94D6F77FBDE36179" modified="2020-08-12" version="1">MVINLNPATQIDAFGLTVASGSGNGEGSGDGNGNHNWNNSGNNNASHITGISNNMMGIFNHALIGGAMYLHVIYVGEMIDKWVTLEEAMNFCHDAVIAYVQLQLELFQNLALDSFEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJX8</accession>
    <name>A0A6I8UJX8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">UDP-glucuronosyltransferase 1-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Ugt36F1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356664.4">
        <property type="nucleotide sequence ID" value="XM_001356628.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJX8"/>
    <dbReference type="FunCoup" id="A0A6I8UJX8">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816869"/>
    <dbReference type="InParanoid" id="A0A6I8UJX8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000144">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF114">
        <property type="entry name" value="IP04436P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026286819" evidence="6">
        <location>
            <begin position="34"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356664.4"/>
        </source>
    </evidence>
    <sequence length="539" mass="61748" checksum="73A3261C2DAEB914" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK67</accession>
    <name>A0A6I8UK67_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vascular endothelial growth factor receptor 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4817169</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356773.4">
        <property type="nucleotide sequence ID" value="XM_001356737.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK67"/>
    <dbReference type="FunCoup" id="A0A6I8UK67">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817169"/>
    <dbReference type="InParanoid" id="A0A6I8UK67"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000677">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF594">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026248414" evidence="6">
        <location>
            <begin position="33"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="435"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="588"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356773.4"/>
        </source>
    </evidence>
    <sequence length="813" mass="90390" checksum="7ECEB8A887B78863" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKQ2</accession>
    <name>A0A6I8UKQ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Selenoprotein BthD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357056.2">
        <property type="nucleotide sequence ID" value="XM_001357020.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKQ2"/>
    <dbReference type="FunCoup" id="A0A6I8UKQ2">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817668"/>
    <dbReference type="InParanoid" id="A0A6I8UKQ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKQ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357056.2"/>
        </source>
    </evidence>
    <sequence length="138" mass="15820" checksum="E3089EA4EF0921F3" modified="2020-08-12" version="1">MSDKTPLDPNQPVLYIDHCRYRENFRRDALQLHVSLAEALRALHPRVKLQLRINEHGPPEEEGAFEVAIAATPAESPSDRQHIWTGLRRVPFAAKVPHVDDIITPVCNALQLVRDDDHTDGESHRRKMANLRRSRSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKR8</accession>
    <name>A0A6I8UKR8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cathepsin E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4817634</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357029.2">
        <property type="nucleotide sequence ID" value="XM_001356993.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKR8"/>
    <dbReference type="FunCoup" id="A0A6I8UKR8">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817634"/>
    <dbReference type="InParanoid" id="A0A6I8UKR8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000008">
        <property type="entry name" value="Cathepsin D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000002">
        <property type="entry name" value="Vacuolar aspartic proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026332972" evidence="9">
        <location>
            <begin position="26"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="96"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="125"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="293"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001357029.2"/>
        </source>
    </evidence>
    <sequence length="415" mass="45124" checksum="B38380D5EDDC3C96" modified="2020-08-12" version="1">MQPLKKVGLGITFLLLALLVASAEAKSSRRKIHRVGLQRDTDQRKILRNLGHQNKMIRSKPRLATAQAETTMFTASSSSSSSIPTETLTNSYNTEYYGQVTIGSQQFQVLFDTASANLWVPSVKCTQKVCLQHNRYNSSRSKTYAANGSAFQIEYATREEAVILEGFLSTDRLKIAGLTVKNQTFAEITSMPESVFNRSNFDGIFGLGFRSISIGDVNPPLLNLFEQGLIEAPLFSLILNRNASEPSNGGQLLLGGSDPTLYSGCLTYVPLSQVGYWQITVGSISLDTDSDLCSNCEAIIDAGTSLIVVPSATLAAINQRFGITAADKRDGVYTISCDKVSSLPALTFNIGRRDFTLPASSYILNYDGTCVSGFTSLSDGGNDLTGLWVLGDVFLGPLYVEFDMEYKRIAIAPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL37</accession>
    <name>A0A6I8UL37_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SET domain-containing protein SmydA-8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SmydA-3</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357232.2">
        <property type="nucleotide sequence ID" value="XM_001357196.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL37"/>
    <dbReference type="KEGG" id="dpo:4818112"/>
    <dbReference type="InParanoid" id="A0A6I8UL37"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UL37">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053010">
        <property type="entry name" value="SET_SmydA-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455:SF7">
        <property type="entry name" value="RE12806P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 4, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001357232.2"/>
        </source>
    </evidence>
    <sequence length="508" mass="56964" checksum="6AECAE1F8730B70F" modified="2020-08-12" version="1">MTSPTSRPVNRTSVQWSAVCGRYLVAQESTPARSLLIEELPFAIGPKCNGPVVCLGCCRPAPATDVEELCVLCGWPLCEECSENEDSPHLQLECREFQISGARFYRLPSGSSYCPQLDCILPLRVLLAKEANPKRWEVEVAPMEHHESERRLNEDVWHADLVNIAQYLRGPCKLASRFSEELIMQVVGVLEVNAFEARTARGYPLRCLYPYTGILAHSCVPNTARSIYPSEGFKIRLRAMVDLDDGQPLHHSYTYTLDGTAQRQTHLREGKFFTCNCERCLDPTELQTHFSSLKCGQCAEGFLAPKQPTEVDTSWNCCSCGASSSNVDVETTIKSIQSEVSGVQALEMGPHRLEEAERLLRKYKSLLHPLHFIATGLRQLLIEMYGRVQRYEMVQLSDDLLERKAELCRQVLSVLNKFEPGLSRSRAMNLYELHVPLVLLAKSGFIASKLNGGELRARLVDAIDLLKECVEILHYEDKCSQEGVLCEVAKQALRQLTLSVEGLGSELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM29</accession>
    <name>A0A6I8UM29_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Triosephosphate isomerase</fullName>
            <ecNumber evidence="6 11">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Tpi</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="6 11"/>
    <dbReference type="RefSeq" id="XP_001357695.4">
        <property type="nucleotide sequence ID" value="XM_001357658.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM29"/>
    <dbReference type="FunCoup" id="A0A6I8UM29">
        <property type="interactions" value="1168"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800416"/>
    <dbReference type="InParanoid" id="A0A6I8UM29"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0023294">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF2">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 11 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001357695.4"/>
        </source>
    </evidence>
    <sequence length="307" mass="33202" checksum="6C438789375E9BD1" modified="2020-08-12" version="1">MDNNPSADISVDLRLSVSSKAHKPTAVFPKLSKRPSEGQKKVSKLLSEILYANFSTNSKMSARKFCVGGNWKMNGDQKSIAEICKTLSGAALDPNTEVVIGCPAIYLMYARNLLPCTVGVAGQNAYKVAKGAFTGEISPSMLKDIGADWVILGHSERRAIFNESDELIAEKAEHALAEGLKVIACIGETLDEREAGKTNEVVARQMCAYAKKVRDWTNVVVAYEPVWAIGTGKTATPEQAQEVHAFLRQWLTDNISKEVSAALRIQYGGSVTAANAKELAKKPDIDGFLVGGASLKPEFVDIINARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM85</accession>
    <name>A0A6I8UM85_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoserine aminotransferase</fullName>
            <ecNumber evidence="12">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4800458</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="12"/>
    <dbReference type="RefSeq" id="XP_001357730.4">
        <property type="nucleotide sequence ID" value="XM_001357693.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM85"/>
    <dbReference type="FunCoup" id="A0A6I8UM85">
        <property type="interactions" value="969"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800458"/>
    <dbReference type="InParanoid" id="A0A6I8UM85"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00244">
        <property type="reaction ID" value="UER00311"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00611">
        <property type="entry name" value="PSAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000010">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000006">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003764">
        <property type="entry name" value="PRK05355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01364">
        <property type="entry name" value="serC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247:SF1">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12 15" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="13">
        <location>
            <begin position="3"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001357730.4"/>
        </source>
    </evidence>
    <sequence length="364" mass="39694" checksum="979BCCD5D7AD614A" modified="2020-08-12" version="1">MVINFAAGPAKLPEEVLKEVQKNLLDCNGSGISVMEMSHRSSSYGKIQETAINDLRELLNIPSNYRVLLMQGGGTGQFAAVALNLIGVTGTADYVITGSWSEKAAKEAAQYGKVNAVLPKEAKYISVPRQNSWKLDPKASYVYYCDNETVEGVEFDFVPEVPAGVPLVADMSSNFLSRPIDVAKFGVIFAGAQKNIGPAGTTVIIVREDLIGKHLKSTPSILNFQLMDKNSSLMNTPPTFVIYVMGLVFKWIKRNGGIAGMSKLANAKSQLIYETIDKSNGFYYCPVDSNVRSHMNVPFRIGSAAGNDALEKEFLAKAEADGMIQLKGHRSVGGIRASLYNAVTLEETQQLANLMVEFYKNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN76</accession>
    <name>A0A6I8UN76_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein fem-1 homolog A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">m-cup</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358214.3">
        <property type="nucleotide sequence ID" value="XM_001358177.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN76"/>
    <dbReference type="FunCoup" id="A0A6I8UN76">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801051"/>
    <dbReference type="InParanoid" id="A0A6I8UN76"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000466">
        <property type="entry name" value="Mann-cup, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000559">
        <property type="entry name" value="Mann-cup, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF82">
        <property type="entry name" value="FI19351P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="135"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="569"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358214.3"/>
        </source>
    </evidence>
    <sequence length="660" mass="75193" checksum="7034D3EDE3315B9B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNB8</accession>
    <name>A0A6I8UNB8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aminopeptidase</fullName>
            <ecNumber evidence="14">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC4800955</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="14"/>
    <dbReference type="RefSeq" id="XP_001358137.2">
        <property type="nucleotide sequence ID" value="XM_001358100.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNB8"/>
    <dbReference type="FunCoup" id="A0A6I8UNB8">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800955"/>
    <dbReference type="InParanoid" id="A0A6I8UNB8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000016">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000013">
        <property type="entry name" value="Aminopeptidase N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14 20" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="8 12 14" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026270847" description="Aminopeptidase" evidence="15">
        <location>
            <begin position="27"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="18">
        <location>
            <begin position="43"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="16">
        <location>
            <begin position="271"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="17">
        <location>
            <begin position="576"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="13">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001358137.2"/>
        </source>
    </evidence>
    <sequence length="980" mass="113450" checksum="509A4D6069D0ACA1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNZ9</accession>
    <name>A0A6I8UNZ9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Synaptosomal-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Snap24</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synapse</location>
            <location evidence="6">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358590.1">
        <property type="nucleotide sequence ID" value="XM_001358553.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNZ9"/>
    <dbReference type="FunCoup" id="A0A6I8UNZ9">
        <property type="interactions" value="917"/>
    </dbReference>
    <dbReference type="GeneID" id="4801511"/>
    <dbReference type="KEGG" id="dpo:4801511"/>
    <dbReference type="InParanoid" id="A0A6I8UNZ9"/>
    <dbReference type="OMA" id="EKCCGVC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081801">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15885">
        <property type="entry name" value="SNARE_SNAP25C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15889">
        <property type="entry name" value="SNARE_SNAP25N_23N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000007">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000018">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000928">
        <property type="entry name" value="SNAP-25_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF14">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00835">
        <property type="entry name" value="SNAP-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="146"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358590.1"/>
        </source>
    </evidence>
    <sequence length="211" mass="23370" checksum="3250A6BDBF107E05" modified="2020-08-12" version="1">MAAVENEPRTELQELQLKSATVADESLESTRRMLSLMDESKEAGIRTLVALDDQGEQLDRIEEGMDRINADMREAEKNLSGMEKCCGICVIPWKKVNIKDDGDSAWKGNDDGKVVASQPQRVIDERERGGMGAPAQSGYVARITNDAREDEMDENLGQVNSMLGNLRNMALDMGSELENQNKQVDRINAKGDANNVRMDGVNKRANNLLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP43</accession>
    <name>A0A6I8UP43_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CUE domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4801583</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CUEDC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358653.2">
        <property type="nucleotide sequence ID" value="XM_001358616.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP43"/>
    <dbReference type="FunCoup" id="A0A6I8UP43">
        <property type="interactions" value="810"/>
    </dbReference>
    <dbReference type="GeneID" id="4801583"/>
    <dbReference type="KEGG" id="dpo:4801583"/>
    <dbReference type="InParanoid" id="A0A6I8UP43"/>
    <dbReference type="OMA" id="ICEWIYK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081916">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14367">
        <property type="entry name" value="CUE_CUED2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039805">
        <property type="entry name" value="CUE_CUED2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12493">
        <property type="entry name" value="CUE DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12493:SF0">
        <property type="entry name" value="CUE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CUE" evidence="8">
        <location>
            <begin position="167"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001358653.2"/>
        </source>
    </evidence>
    <sequence length="303" mass="34400" checksum="8366A85A15061E55" modified="2020-08-12" version="1">MTNLEKQHEMVKHSLVEFISGHIPGADFRVVDEIVLSYIISILEEASQDPCFDVEGFVEMMGAYFEEFPTIDQGVICDWIYKLANELTEMEKNQGNHDAVDLSLNLLSLSNIIPESRLRARNSSISEKDELSSNNSSSGGSHGKRSQHLSETSDGGSTDSSSSNCDYFFDEAEALQEMFPDAAYVEIKHCIAISKGDIDSATQILLHRQETNQSLTDKSHSMIIKKNVVVDDNELKNRIIARYSYVDKNATQREYKPVVPKMEPRKLVRYRDNKIVSLKGERYTEIKRDEDAELKKPKKQIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPL7</accession>
    <name>A0A6I8UPL7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inositol polyphosphate 1-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Ipp</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358702.3">
        <property type="nucleotide sequence ID" value="XM_001358665.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPL7"/>
    <dbReference type="FunCoup" id="A0A6I8UPL7">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801649"/>
    <dbReference type="InParanoid" id="A0A6I8UPL7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004441">
        <property type="term" value="F:inositol-1,4-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01640">
        <property type="entry name" value="IPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.460.10:FF:000001">
        <property type="entry name" value="Inositol polyphosphate 1-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.460.10">
        <property type="entry name" value="Inositol Polyphosphate 1-phosphatase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044897">
        <property type="entry name" value="INPP1_dom_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF3">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE 1-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358702.3"/>
        </source>
    </evidence>
    <sequence length="372" mass="40431" checksum="2A65D78E30E9F9E8" modified="2020-08-12" version="1">MGSEDKANLLRVLINCAEKAANIARTCRANEQLLSLLVQEKSEGEANERFEHDFKTLADVLIQETIKHEVGAVFPEMKAAILGEESPNFTNKLGESVTVAVGACEADTTACLEAVLSGHESAASALADEVHRVVNFESGTLGDIPELPEELDYANLGIWIDPIDATAEYISGDTMFTNFPGITSTGLDCVTVLIGVYERDTGIPVIGVVAQPFGNKLDEHVYSSSMFWGICLPSLQAHNCNFEARDEHRQLAIFSSSEQSDILQRFLDLGYEFAFSAGAGHKALKVITREVEVYLLSKGSTFKWDTCAPQAILRALGGDVFDYGASVAELKTVPLKYLMQDSGSDWKRNATGIIAVRNVDAMEVLLAKLAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQF1</accession>
    <name>A0A6I8UQF1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359285.1">
        <property type="nucleotide sequence ID" value="XM_001359248.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQF1"/>
    <dbReference type="GeneID" id="4802357"/>
    <dbReference type="KEGG" id="dpo:4802357"/>
    <dbReference type="InParanoid" id="A0A6I8UQF1"/>
    <dbReference type="OMA" id="NYAPCFL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071317">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF13">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="158"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359285.1"/>
        </source>
    </evidence>
    <sequence length="439" mass="50195" checksum="47CE6D958E3D0A75" modified="2020-08-12" version="1">MSVSDLIGALDAIPKLSGSAAPSTNSNSVIMDAPDWLTESYIQDALRDFYGDQRIKITCLQVHPALGKGENYGGVLTRVKAQFTRRDGTCQIGHYIIKSSFESDEFSRNAMEPYDIFNREMSIYEQILPKLKALLHEIEDGEEIFAETMAVDYDRSVLIFEDLSARKFVMPDRLTGLDLKLARLVLRKLAKMHATSAVLNEREDHALESYDRGMFNRHTDNYAPCFLGMLQAASRRVAQWPGYELYAEKLEALLPVYMELGKRVFDVSPGQINVLAHGDLWTNNVLVKYDKETGEAEDVILIDFQYVAWGSPAIDLFYFLNSSLEFDLHLNNQEELIHYYFNIFSETLRKLRFKAAIPSMHQFHQQLQEKAFYGMHTTISILPIQRNVESADADFNALMQSNQRAINFKNACYGNPATQRIMRQLLPKFHQEGLLDADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQX8</accession>
    <name>A0A6I8UQX8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 200</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4802368</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359297.4">
        <property type="nucleotide sequence ID" value="XM_001359260.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQX8"/>
    <dbReference type="FunCoup" id="A0A6I8UQX8">
        <property type="interactions" value="489"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802368"/>
    <dbReference type="InParanoid" id="A0A6I8UQX8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002534">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000325">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="14">
        <location>
            <begin position="11"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="399"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="228"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001359297.4"/>
        </source>
    </evidence>
    <sequence length="439" mass="50752" checksum="BCC6D7D1E3990251" modified="2020-08-12" version="1">MDENAASAVKLKCRTCLGEFEEVTMSALFEADEADVDAEDAGNEDLSEKFQFCCGIKIRNSHRLPSKVCSKCREFVYMWFRFRTMVLNSQVYLETCFSDGEQAEDFKQVSNSEFMKHMYQNLQVNCGLENQYQDDSPQEELDDITAQYQEIFDGVNYVTDNEELVANEPVTDADDQPKEQAEEIMIVNVQPFDQDLVADETITPGDDGQIVEDSPIMNEDYCDVDDFDDSNIDFLSPTPSPEPKPSTAQNKRRPGRPRKPDNELKCKRKNTSPGEPETEFKRLPTKFICSLCGNVYHKKSIFTSHMMAHADYKPHQCEICDKSFRQMGELRAHIRRHTGERPYKCMYCERHFYDRSERVRHERVHTNTRPYECKECGKAFTHPAILKNHSLVHSGEKNYNCAVCSKSFTLMHQLKAHQQTIIHRNMEEQAMANSTDHTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URF6</accession>
    <name>A0A6I8URF6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Short-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="4">1.3.8.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Butyryl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Arc42</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:24005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43456"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57389"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:86160"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:43457"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001359731.2">
        <property type="nucleotide sequence ID" value="XM_001359694.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URF6"/>
    <dbReference type="SMR" id="A0A6I8URF6"/>
    <dbReference type="FunCoup" id="A0A6I8URF6">
        <property type="interactions" value="994"/>
    </dbReference>
    <dbReference type="GeneID" id="4802905"/>
    <dbReference type="KEGG" id="dpo:4802905"/>
    <dbReference type="InParanoid" id="A0A6I8URF6"/>
    <dbReference type="OMA" id="LYREAPM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0064447">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016937">
        <property type="term" value="F:short-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046359">
        <property type="term" value="P:butyrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01158">
        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
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        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
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        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
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        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0274">FAD</keyword>
    <keyword evidence="5 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="17">
        <location>
            <begin position="29"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="16">
        <location>
            <begin position="144"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="15">
        <location>
            <begin position="253"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001359731.2"/>
        </source>
    </evidence>
    <sequence length="405" mass="43387" checksum="90230CCC64DD3B63" modified="2020-08-12" version="1">MQSVLSRSLNIARRSVPMRQIASIAALPDTHQMLQKTCRDFANAELSPNAAKFDREHLYPAQQIKQMGELGVMSVAIPEELGGTGLDYLAYAIAMEEISRGCASAGVIMSVNNSLYLGPLLGFGNAAQKEAFITPYTTGERVGCFALSEPGNGSDAGAASTVATEKGDHYVLNGTKAWITNGFEAGAAVVFATTNKQLKHKGISAFIVPKGLKGFSLGKKEDKLGIRGSSTCQLIFEDCEIPKDSMLGEPGFGFKIAMQTLDAGRIGIAGQALGIGQAALELAVDYAQKRQAFGKPISKLQSIQQKIADMSLAMESARLLTWRAAWLKDNKQAYTKEAAMAKLAASEAATLCSHQCIQILGGMGYVADMPAERHYRDARITEIYEGTSEIQRLVIAGSILKEYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URV8</accession>
    <name>A0A6I8URV8_DROPS</name>
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        <submittedName>
            <fullName evidence="10">GRIP and coiled-coil domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GCC88</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359940.2">
        <property type="nucleotide sequence ID" value="XM_001359903.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URV8"/>
    <dbReference type="FunCoup" id="A0A6I8URV8">
        <property type="interactions" value="1167"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A6I8URV8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GG17233"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.60">
        <property type="entry name" value="GRIP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051952">
        <property type="entry name" value="Golgi-autophagy_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23157">
        <property type="entry name" value="GRIP AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23157:SF25">
        <property type="entry name" value="GRIP AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01465">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00755">
        <property type="entry name" value="Grip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="8">
        <location>
            <begin position="693"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="101"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="594"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="170"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="469"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359940.2"/>
        </source>
    </evidence>
    <sequence length="747" mass="84814" checksum="F8E7DEE3434D7F96" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USP6</accession>
    <name>A0A6I8USP6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Luciferin 4-monooxygenase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4803281</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360047.2">
        <property type="nucleotide sequence ID" value="XM_001360010.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USP6"/>
    <dbReference type="KEGG" id="dpo:4803281"/>
    <dbReference type="InParanoid" id="A0A6I8USP6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000025">
        <property type="entry name" value="luciferin 4-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="38"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="446"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360047.2"/>
        </source>
    </evidence>
    <sequence length="539" mass="61017" checksum="B5FB184519C0C10C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USQ0</accession>
    <name>A0A6I8USQ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methionine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MetRS</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001360316.2">
        <property type="nucleotide sequence ID" value="XM_001360279.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USQ0"/>
    <dbReference type="FunCoup" id="A0A6I8USQ0">
        <property type="interactions" value="1730"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803628"/>
    <dbReference type="InParanoid" id="A0A6I8USQ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00939">
        <property type="entry name" value="MetRS_RNA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000010">
        <property type="entry name" value="methionine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000014">
        <property type="entry name" value="Methionyl-tRNA synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041598">
        <property type="entry name" value="MARS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18485">
        <property type="entry name" value="GST_N_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00458">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51185">
        <property type="entry name" value="WHEP_TRS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="GST C-terminal" evidence="16">
        <location>
            <begin position="66"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="WHEP-TRS" evidence="17">
        <location>
            <begin position="824"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="WHEP-TRS" evidence="17">
        <location>
            <begin position="889"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="WHEP-TRS" evidence="17">
        <location>
            <begin position="961"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="890"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001360316.2"/>
        </source>
    </evidence>
    <sequence length="1021" mass="112267" checksum="A7AE63729D7FA6F1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USV0</accession>
    <name>A0A6I8USV0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein Aster-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4803702</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360377.4">
        <property type="nucleotide sequence ID" value="XM_001360340.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USV0"/>
    <dbReference type="FunCoup" id="A0A6I8USV0">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803702"/>
    <dbReference type="InParanoid" id="A0A6I8USV0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VASt" evidence="4">
        <location>
            <begin position="122"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360377.4"/>
        </source>
    </evidence>
    <sequence length="303" mass="33792" checksum="9A58CCB7FAB1FE53" modified="2020-08-12" version="1">MEALRFRFGKWGGAKRSKSTSKPLTRNTTAASSRSISRATNASLTSLARRRRTTRHMKAKSVAFDQNNYVVAGGKAKKRMSNARPSNADQGITTKRGMTVVEVIMPIRDSLEGKYNCNALHEGRKVLNEKMRVHVDTLFNMLFSATSPFFQKFHEMRNSTDLKLGEWRKNAAGLNERVITVTVALKGNVGPKVAKVTETQTLRDCTRPGQLYSIDATSVNADIPYADSFMVAMHLCLRGTPEGHTKILVLAQVKIVKPVWAVVQSFIEKHTYAGVEEFFQSLYSALLRDQRRTTTLPASGSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USZ2</accession>
    <name>A0A6I8USZ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4803765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360428.2">
        <property type="nucleotide sequence ID" value="XM_001360391.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USZ2"/>
    <dbReference type="FunCoup" id="A0A6I8USZ2">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803765"/>
    <dbReference type="InParanoid" id="A0A6I8USZ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360428.2"/>
        </source>
    </evidence>
    <sequence length="341" mass="39305" checksum="B5EA04862A43FB69" modified="2020-08-12" version="1">MDCNNNNKAPHLSIPVMQGAGSHLTTTESAVQVALHNLVVMDESVEGTVLGVLLSAEKKYRLTFDTFKKEMELQAIAASTLWELIQRYKIAMEVDADCKCTQLYAVEPKEETIQKYYIHYQVIVSSNKSLWRQIESLRSYVLAFRRECKNLNLEEQTPFIQGDAFHKPIKFFIELVDELFNYFFSLHLKLDCAARQLDPMDLESLEEYQKLLKPNEDFEEYFLYNLSYCQCLRMPPKCPETRVYTNIENAAMKKEAMEQAKRARCDQRVNRMTLRASQMRESAENPPSVTQDTELAPNPDAPPESGNATSDPLSAKFERQLISERHTRQLVNQVHNALSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTA2</accession>
    <name>A0A6I8UTA2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Ugt49B1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001360427.4">
        <property type="nucleotide sequence ID" value="XM_001360390.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTA2"/>
    <dbReference type="FunCoup" id="A0A6I8UTA2">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803762"/>
    <dbReference type="InParanoid" id="A0A6I8UTA2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000174">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF60">
        <property type="entry name" value="UDP-GLUCURONOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026379910" description="UDP-glucuronosyltransferase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="487"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360427.4"/>
        </source>
    </evidence>
    <sequence length="527" mass="59710" checksum="9676CFD6A21AABD6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUW2</accession>
    <name>A0A6I8UUW2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Serine proteinase stubble isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4804771</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361281.3">
        <property type="nucleotide sequence ID" value="XM_001361244.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUW2"/>
    <dbReference type="KEGG" id="dpo:4804771"/>
    <dbReference type="InParanoid" id="A0A6I8UUW2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UUW2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000006">
        <property type="entry name" value="Serine proteinase stubble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF65">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="394"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="208"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="237"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001361281.3"/>
        </source>
    </evidence>
    <sequence length="639" mass="69827" checksum="D9E0B7D57C65A6D9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV08</accession>
    <name>A0A6I8UV08_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil-helix-coiled-coil-helix domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4804705</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361223.1">
        <property type="nucleotide sequence ID" value="XM_001361186.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV08"/>
    <dbReference type="FunCoup" id="A0A6I8UV08">
        <property type="interactions" value="1242"/>
    </dbReference>
    <dbReference type="GeneID" id="4804705"/>
    <dbReference type="KEGG" id="dpo:4804705"/>
    <dbReference type="InParanoid" id="A0A6I8UV08"/>
    <dbReference type="OMA" id="ATCLTEM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071902">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005761">
        <property type="term" value="C:mitochondrial ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033620">
        <property type="entry name" value="Ribosomal_mS37_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31278">
        <property type="entry name" value="CHCHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31278:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHCH" evidence="2">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361223.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="13831" checksum="9D02813B54A7624E" modified="2020-08-12" version="1">MRIPGALYAARGRAPQSEKDVPFQEILPLRLKNTVSGKADSGSDVACLQEMGMLFACMKDNEFVEKYCHKEIQQFQNCFKCYMDRKFEAKKTVNQGVVQPGNNLNYKQLNKYMRRFPNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW02</accession>
    <name>A0A6I8UW02_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras-related protein Rab-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Rab3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361745.1">
        <property type="nucleotide sequence ID" value="XM_001361708.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW02"/>
    <dbReference type="SMR" id="A0A6I8UW02"/>
    <dbReference type="FunCoup" id="A0A6I8UW02">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="GeneID" id="4805325"/>
    <dbReference type="KEGG" id="dpo:4805325"/>
    <dbReference type="InParanoid" id="A0A6I8UW02"/>
    <dbReference type="OMA" id="PSSQCNC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080445">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01865">
        <property type="entry name" value="Rab3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000206">
        <property type="entry name" value="Ras-related protein Rab-3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037872">
        <property type="entry name" value="Rab3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361745.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24876" checksum="B324A77DBA2367A1" modified="2020-08-12" version="1">MASGGDPKWQKDAADQNFDYMFKLLIIGNSSVGKTSFLFRYADDSFTSAFVSTVGIDFKVKTVFRHDKRVKLQIWDTAGQERYRTITTAYYRGAMGFILMYDVTNEDSFNSVQDWVTQIKTYSWDNAQVILVGNKCDMEDQRVISFERGRQLADQLGVEFFETSAKENVNVKAVFERLVDIICDKMSESLDADPTLVGGAQKGQRLTDQPQGTPNANCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW61</accession>
    <name>A0A6I8UW61_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4805445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361846.3">
        <property type="nucleotide sequence ID" value="XM_001361809.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW61"/>
    <dbReference type="KEGG" id="dpo:4805445"/>
    <dbReference type="InParanoid" id="A0A6I8UW61"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026149296" description="C-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="157"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361846.3"/>
        </source>
    </evidence>
    <sequence length="321" mass="35914" checksum="1EC5E3F82915901C" modified="2020-08-12" version="1">MIITLIFLLLQLLASLSMGSLNCPTDEAIPPQFHLVRECQRASADDALSLENTSSFEDCANLARELHGLALNYAPGGPTRHRNMFIQSNHGSHGRILRSRLTVFEQPAEFFNCHVLQCPQNSTFGGMVNDSRFDYYSLHGRPTTLRNYSCVPEVGLFLVYTNPSSYLNASLQCSNSSEFSGSLAHIASESRTRALSQWLMDFNRKSRPVLEAKPMPIIFLAYVGLAFNRTTSLNAHDFRNAQHESLICFLYRAWDVGHPALGQQKANESCVALTPQGTWHALSCERQLPFICEIFTASPRSWKHEAELDIGPNSVAKCRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW82</accession>
    <name>A0A6I8UW82_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Testis-expressed protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4805447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361847.2">
        <property type="nucleotide sequence ID" value="XM_001361810.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW82"/>
    <dbReference type="KEGG" id="dpo:4805447"/>
    <dbReference type="InParanoid" id="A0A6I8UW82"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313:SF0">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23313">
        <property type="entry name" value="TSEC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361847.2"/>
        </source>
    </evidence>
    <sequence length="397" mass="44937" checksum="94F9F4E9D8FABB48" modified="2020-08-12" version="1">MAELLSREKELFKMNQELNLLTLTPAGMADVIYPAQGPSGLTAGLTRTRNGTFHRQKGPSTLLKKKCAQTKLAPKAMGAGSVSPSARNSPPPPAPASTLPSSSKTSDWKKGKSTGTPSPTTARFDSKFSTFTRGQSSKTATIVKYRNPNFSDSTSLDMILLDNVSVQSDTMDMEVPKRRESTTVVNGQAKKQLTQDNFIKFLKAKVAILEEDHAQHASELDRQKEKLDQALEGMRKSESQRDQSLHSNKHLSEQVQRAEQQCEEANRRLKERQQEFTSQQRELEQLKRENKVLKQGNMNLENRLTRTQEEAESARQALNKIREEQRDELDSHRNELKTKESRIKALKRQRGDLLNAYKKQLYMIDNLKRQTSCMEQSVAIGCGEKEFNKVLEWNAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX94</accession>
    <name>A0A6I8UX94_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Accessory gland protein Acp53Ea-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4805681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001362040.2">
        <property type="nucleotide sequence ID" value="XM_001362003.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX94"/>
    <dbReference type="FunCoup" id="A0A6I8UX94">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="GeneID" id="4805681"/>
    <dbReference type="KEGG" id="dpo:4805681"/>
    <dbReference type="InParanoid" id="A0A6I8UX94"/>
    <dbReference type="OMA" id="WLACNRI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243575">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009392">
        <property type="entry name" value="ACP53EA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06313">
        <property type="entry name" value="ACP53EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026276174" evidence="1">
        <location>
            <begin position="20"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001362040.2"/>
        </source>
    </evidence>
    <sequence length="120" mass="13697" checksum="9A903FC7BF7B240E" modified="2020-08-12" version="1">MNLLKAALLLSALVVLSEAGEESGSIDWEKWLECTHIGARASAQILRRTIPAMRVLYQCIDFEPLRDPEFSQLRLLKNIYKFLKLSVYDKQSCLLDPLKGVVNTLEPYVERIDSMHCLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXM7</accession>
    <name>A0A6I8UXM7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fucosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6903002</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus</location>
            <location evidence="1 12">Golgi stack membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_002132724.2">
        <property type="nucleotide sequence ID" value="XM_002132688.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXM7"/>
    <dbReference type="KEGG" id="dpo:6903002"/>
    <dbReference type="InParanoid" id="A0A6I8UXM7"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008417">
        <property type="term" value="F:fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000004">
        <property type="entry name" value="Glycoprotein 3-alpha-L-fucosyltransferase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48438">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48438:SF1">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="15">
        <location>
            <begin position="154"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="14">
        <location>
            <begin position="284"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002132724.2"/>
        </source>
    </evidence>
    <sequence length="481" mass="57057" checksum="79CAE4D656340423" modified="2020-08-12" version="1">MAPRNLSLKLCTCFTLICALLLIYRLNLEVYEVWQTLSTRTYVEIPQHLERKVQEYVDHQEQEKPKEEEKIEKGEKLEKEEKIYDEPSSGLPWFFKKGKYYPPPSRAHSNHSLQKQRDAPKLLPHQDPHKDRIVNQLMYVPHDYAKIKASGRLKTILLYNGRRTWNVKKGRDLFLKDKCPVDTCEVTDDMGLAHTADMILYKNLYVPTKKLSSAREIRAKNTKQIKMLYFLESPVYTQNTKVPTGINWTATYRRDSTIVTPYSKWVYFDAKVQQKKQDRNYAANRTRKVAWFVSNCNTENGRFKYALELQKYIEVDIYGFCGKLKCDRFDPEKCFKLLENNYKFYLAFENSNCRDYITEKYHQNALNRGVLPIVMGARPGDYKDLAPLNSYIHVDDFASPKELADYLKVLDDDDDLYNSFFQWKGTGEFINTHFWCRVCAMLHNEEQLREPHFYSDYNHWWRGPGVCSPFSWRSKTLFEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ88</accession>
    <name>A0A6I8UZ88_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6901231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133932.2">
        <property type="nucleotide sequence ID" value="XM_002133896.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ88"/>
    <dbReference type="FunCoup" id="A0A6I8UZ88">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901231"/>
    <dbReference type="InParanoid" id="A0A6I8UZ88"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ88">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026311997" evidence="1">
        <location>
            <begin position="23"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133932.2"/>
        </source>
    </evidence>
    <sequence length="208" mass="22268" checksum="65FA221ABBA2A6CA" modified="2020-08-12" version="1">MKAITLCLLVLVSASCLLTTHANAVGSSENFDYDLEIEQLLADLEGDLDYMGVAEQGFIRSCRGILRKALKGIRGTNCILKETLAVLTACTTYVDAIDSCGVAVPKDVANILKTVKSMITICNSIIHLHSQLCATEDTTTTGTGKTATKCFLKLFRATMSLVRKINTTLKLASKLPSDTSSCFVGATNTVKDACNAFLPNIDVCIDSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V054</accession>
    <name>A0A6I8V054_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein stg1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">stg1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134559.2">
        <property type="nucleotide sequence ID" value="XM_002134523.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V054"/>
    <dbReference type="KEGG" id="dpo:6901083"/>
    <dbReference type="InParanoid" id="A0A6I8V054"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016247">
        <property type="term" value="F:channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099590">
        <property type="term" value="P:neurotransmitter receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098943">
        <property type="term" value="P:neurotransmitter receptor transport, postsynaptic endosome to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051968">
        <property type="term" value="P:positive regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098970">
        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019226">
        <property type="term" value="P:transmission of nerve impulse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000057">
        <property type="entry name" value="GH12419p1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051072">
        <property type="entry name" value="CACNG_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107:SF0">
        <property type="entry name" value="STARGAZIN (MAMMALIAN CALCIUM CHANNEL) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="465"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="621"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="708"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="902"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="468"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="717"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="927"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002134559.2"/>
        </source>
    </evidence>
    <sequence length="999" mass="110538" checksum="1F8CB249E95669D5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0L4</accession>
    <name>A0A6I8V0L4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cathepsin L-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6900590</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134963.2">
        <property type="nucleotide sequence ID" value="XM_002134927.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0L4"/>
    <dbReference type="KEGG" id="dpo:6900590"/>
    <dbReference type="InParanoid" id="A0A6I8V0L4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cathepsin L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
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        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026264535" evidence="3">
        <location>
            <begin position="22"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="5">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="4">
        <location>
            <begin position="114"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002134963.2"/>
        </source>
    </evidence>
    <sequence length="323" mass="35998" checksum="A265668E6F711A20" modified="2020-08-12" version="1">MQSQVLIFLFCLLTTVRGAAAWSEWNAFKERHNKSYQSADEHRLRFLIFMDNKLRIAEHNKRWARGQESHQLGINQFADLSSREFRERLLHSEQVSQGFGDVYLMPSEVEIEPLPETVDWRTRNAVTAVKSQGDFPTCWSFAATGVVEGRHAIKYGHLQSLSEQNLIDCCLGSEGGYNTWRAVQCIRTLGGIDTEASYPYQGPAGPCSYRPSARAARVFGAVPLPQGNERTMAQALLSGPLAVSINAATIQFYRSGVFRDSSCSRSVNHAVLAVGYGTDPSYGDYWLIKNSWGTGWGESGYIRMARNSDNQCGVASSVFYPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0N6</accession>
    <name>A0A6I8V0N6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6900638</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 11">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135011.2">
        <property type="nucleotide sequence ID" value="XM_002134975.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0N6"/>
    <dbReference type="KEGG" id="dpo:6900638"/>
    <dbReference type="InParanoid" id="A0A6I8V0N6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF7">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="242"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="10">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
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        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002135011.2"/>
        </source>
    </evidence>
    <sequence length="529" mass="59524" checksum="6F8298C24FEDF1FB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1Y8</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein C7orf50</fullName>
        </submittedName>
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        <name evidence="5" type="primary">LOC6900866</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135223.3">
        <property type="nucleotide sequence ID" value="XM_002135187.3"/>
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    <dbReference type="FunCoup" id="A0A6I8V1Y8">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900866"/>
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        <property type="component" value="Chromosome X"/>
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            <begin position="173"/>
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        </location>
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            <begin position="1"/>
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        </location>
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        <location>
            <begin position="229"/>
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            <begin position="1"/>
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        <location>
            <begin position="11"/>
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    </feature>
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        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="154"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002135223.3"/>
        </source>
    </evidence>
    <sequence length="272" mass="31214" checksum="42FA71CCAE26733C" modified="2020-08-12" version="1">MARSEVETKPKPKGKKRKHEKAAAASNNSSDDEVATKSLIVEPEMEEQAQRAGKRRQKENDALAKQKRIKQTPVAVAPEEVEDNPEISPRKVAKRKQREEAAALKAKRSKDDGDDDEENEESLPTDAQLQEASKPENTKAIVTVRQKKKQKHQQRLEAQKDQNSNKEAKRNEEYLLQWKNSRAEWKFVKLRQISIQQTAFDEEKLAAEIWDIALEYLASSQGAARAKISKLADEVIEKLDKEGEKLEDEAERQKLIESTRYQRARDLLQSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V201</accession>
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        <recommendedName>
            <fullName evidence="1">G patch domain-containing protein 4</fullName>
        </recommendedName>
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        <name evidence="5" type="primary">LOC6896662</name>
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    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">MV2-25</strain>
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                <consortium name="RefSeq"/>
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            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
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    <dbReference type="OMA" id="ILGKYGW"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248273">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF9">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 4"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01585">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
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            <begin position="187"/>
            <end position="273"/>
        </location>
    </feature>
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        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
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        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00040365"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002136877.1"/>
        </source>
    </evidence>
    <sequence length="273" mass="30831" checksum="A75993082730FF7B" modified="2020-08-12" version="1">MDFAKKILNKYGWKEGEGLGKNNTGIAAPLKASLKFDNAGLGVDRAQEFNDHWWERCFNEAANNVDVQVQQNGQVSTNRKQGEEAVEISTKGFSARKLRKAKEQQAVAGAATYDNFLQTALLTQNGGEVDNTERIKVEDIAVTKVSVLTDEELFKACGGRTAHKGARHGLKLSGKLARIEQQEREMLEKLQSRQKSVLDEEPTKKKKKRDKSEQTQELQEEAEEQPEDVGAEPVEEPVKYKKKKRKTEESIETEAEPAVKSKKKHKHRQEDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2D4</accession>
    <name>A0A6I8V2D4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Disintegrin and metalloproteinase domain-containing protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Meltrin</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137337.3">
        <property type="nucleotide sequence ID" value="XM_002137301.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V2D4">
        <property type="interactions" value="259"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897157"/>
    <dbReference type="InParanoid" id="A0A6I8V2D4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF159">
        <property type="entry name" value="ADAM METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 16" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026230783" evidence="11">
        <location>
            <begin position="35"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="14">
        <location>
            <begin position="285"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="13">
        <location>
            <begin position="487"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="725"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="218"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="820"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="913"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1027"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1222"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1299"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="224"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="933"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1027"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1070"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1125"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1236"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1257"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1299"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1328"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1375"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1408"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="396"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="438"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="729"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="747"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002137337.3"/>
        </source>
    </evidence>
    <sequence length="1420" mass="152178" checksum="7A0AE9052980652D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2H4</accession>
    <name>A0A6I8V2H4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein KIBRA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein kibra</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">kibra</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. Kibra acts synergistically along with Ex and Mer to regulate the Hippo signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Forms a complex with Mer and Ex. Interacts (via domain WW 1) with Ex (via RXPPXY motif). Interacts with Mer, Sav, Hpo and Wts.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WWC family. KIBRA subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137203.2">
        <property type="nucleotide sequence ID" value="XM_002137167.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V2H4">
        <property type="interactions" value="315"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897018"/>
    <dbReference type="InParanoid" id="A0A6I8V2H4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046621">
        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035330">
        <property type="term" value="P:regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000228">
        <property type="entry name" value="Blast:Protein kibra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051105">
        <property type="entry name" value="WWC/KIBRA_Hippo_Reg"/>
    </dbReference>
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        <property type="entry name" value="WWC1_hairpin"/>
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        <property type="entry name" value="BOMB/KIRA PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN KIBRA"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="WW"/>
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        <property type="entry name" value="WWC1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WW"/>
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    </dbReference>
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        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
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        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="94"/>
            <end position="127"/>
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        <location>
            <begin position="697"/>
            <end position="817"/>
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        <location>
            <begin position="1"/>
            <end position="50"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="520"/>
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            <begin position="847"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="895"/>
            <end position="934"/>
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        <location>
            <begin position="1168"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1217"/>
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            <begin position="273"/>
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            <begin position="334"/>
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        <location>
            <begin position="393"/>
            <end position="420"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
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            <begin position="529"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
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            <begin position="847"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="866"/>
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        <location>
            <begin position="906"/>
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        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1179"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1234"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010585"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013712"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00024960"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025969"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067981"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_002137203.2"/>
        </source>
    </evidence>
    <sequence length="1284" mass="143756" checksum="AF7BE8203B6DC8DA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V377</accession>
    <name>A0A6I8V377_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6897737</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits VhaAC45 and ATP6AP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Vacuole membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_002137807.3"/>
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    <dbReference type="GeneID" id="6897737"/>
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        <property type="component" value="Chromosome 2"/>
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    <dbReference type="Bgee" id="FBgn0247680">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
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        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
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    </dbReference>
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        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
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        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
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        <property type="entry name" value="ATPase_proteolipid_csu"/>
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    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
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        <property type="entry name" value="F/V-ATP_Csub_sf"/>
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        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
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    </dbReference>
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        <property type="entry name" value="VACATPASE"/>
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        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="11">
        <location>
            <begin position="20"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="11">
        <location>
            <begin position="96"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137843.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="16114" checksum="FA52E17E63E9AECB" modified="2020-08-12" version="1">MYDAGLQETSPPYAIFLGGVGAAIAIIFPALGAAYGTAVSGMGIAQMAVSKPEMLMKSLIPVVMAGIIAIYGLVVSVLIAGSINDSYTVHDGFIHLGAGLSVGLPGLAAGIAIGIAGDAGVRGTGQQPRLFVGMILILIFAEVLGLYGLIVAIYLYTKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3I7</accession>
    <name>A0A6I8V3I7_DROPS</name>
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        <recommendedName>
            <fullName evidence="5">Protein CIP2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137998.2">
        <property type="nucleotide sequence ID" value="XM_002137962.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3I7"/>
    <dbReference type="FunCoup" id="A0A6I8V3I7">
        <property type="interactions" value="494"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897896"/>
    <dbReference type="InParanoid" id="A0A6I8V3I7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042510">
        <property type="entry name" value="CIP2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23161">
        <property type="entry name" value="PROTEIN CIP2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23161:SF2">
        <property type="entry name" value="PROTEIN CIP2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="612"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="833"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137998.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="877" mass="99763" checksum="53FB9C1791A2EC24" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3N7</accession>
    <name>A0A6I8V3N7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peroxiredoxin-5</fullName>
            <ecNumber evidence="9">1.11.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Prx5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="9"/>
    <dbReference type="RefSeq" id="XP_002138062.1">
        <property type="nucleotide sequence ID" value="XM_002138026.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3N7"/>
    <dbReference type="FunCoup" id="A0A6I8V3N7">
        <property type="interactions" value="1184"/>
    </dbReference>
    <dbReference type="GeneID" id="6897963"/>
    <dbReference type="KEGG" id="dpo:6897963"/>
    <dbReference type="InParanoid" id="A0A6I8V3N7"/>
    <dbReference type="OMA" id="SAWGKQH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247537">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000020">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF16">
        <property type="entry name" value="PEROXIREDOXIN-5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="34"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="8">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002138062.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="19777" checksum="7560CBDB603A0317" modified="2020-08-12" version="1">MRVLSCKILGRVVNSTLSTVNPVRSFSRNLPAMVKVGDALPAVDLFEDSPANKINTGDLVNGKKVIIFGVPGAFTPGCSKTHLPGYVSSADELKSKQGVDEIVCVSVNDPFVMSAWGKEHGAGGKVRLLADPAGGFTKALDVSIDLPPLGGVRSKRYSLVVENGKVTELNVEPDGTGLSCSLANNIGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3Z6</accession>
    <name>A0A6I8V3Z6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">transketolase</fullName>
            <ecNumber evidence="8">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6897425</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="8"/>
    <dbReference type="RefSeq" id="XP_002137577.3">
        <property type="nucleotide sequence ID" value="XM_002137541.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3Z6"/>
    <dbReference type="FunCoup" id="A0A6I8V3Z6">
        <property type="interactions" value="618"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897425"/>
    <dbReference type="InParanoid" id="A0A6I8V3Z6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000028">
        <property type="entry name" value="Transketolase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000033">
        <property type="entry name" value="Transketolase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051424">
        <property type="entry name" value="Transketolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004559">
        <property type="entry name" value="PRK05899.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195:SF1">
        <property type="entry name" value="FI06132P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195">
        <property type="entry name" value="TRANSKETOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="14">
        <location>
            <begin position="381"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002137577.3"/>
        </source>
    </evidence>
    <sequence length="688" mass="75050" checksum="5FD3FBEDDCE01DEF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4F0</accession>
    <name>A0A6I8V4F0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138592.2">
        <property type="nucleotide sequence ID" value="XM_002138556.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4F0"/>
    <dbReference type="KEGG" id="dpo:6898572"/>
    <dbReference type="InParanoid" id="A0A6I8V4F0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026322269" evidence="2">
        <location>
            <begin position="25"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138592.2"/>
        </source>
    </evidence>
    <sequence length="344" mass="37803" checksum="285E8D8C4B96C60C" modified="2020-08-12" version="1">MSKFNVWLIFWCAVMMMQPWMNQSHALAADAVNATSVQSTIGLLMGEATTAKTTASPQAVVKSSTRQEAAKEIVAGAPTVIPKTDPVTGPAGPKIEKARLGSSVRLITGLLSGLHRTKRRLSLLNDTLMPRSGPHKGDHASSAPVDTTTSAPPTMMDDSEQQELDYQPQMVAKPMAEDFPLNEPENDPHGGHTMTSSKYRCMHMSSPVLPPIFFMLQQLLQPSPMMETATEMHHSPITSPLFELLNDIEDMTLQNIPNAIERLLANRFVFASGTQLDNKDKNSTQQDDDVSENDELKKEAAEVAPTHDKDEIVLVCPLHHEHHANHNGEIIDDNVILVDQCHVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V563</accession>
    <name>A0A6I8V563_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box/LRR-repeat protein fbxl-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FipoQ</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736251.1">
        <property type="nucleotide sequence ID" value="XM_003736203.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V563"/>
    <dbReference type="FunCoup" id="A0A6I8V563">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="4800480"/>
    <dbReference type="InParanoid" id="A0A6I8V563"/>
    <dbReference type="OMA" id="AKIHIDQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075208">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V563">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000556">
        <property type="entry name" value="CLUMA_CG018969, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000432">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000498">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20933:SF4">
        <property type="entry name" value="F-BOX INVOLVED IN POLYQ PATHOGENESIS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20933">
        <property type="entry name" value="F-BOX ONLY PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="31"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_003736251.1"/>
        </source>
    </evidence>
    <sequence length="515" mass="58913" checksum="2D491C9B8A8C30A0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8M7</accession>
    <name>A0A6I8V8M7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Potassium voltage-gated channel protein Shaw isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Shaw</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Shaw sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015036102.2">
        <property type="nucleotide sequence ID" value="XM_015180616.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V8M7"/>
    <dbReference type="FunCoup" id="A0A6I8V8M7">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V8M7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V8M7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18416">
        <property type="entry name" value="BTB_Shaw-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000020">
        <property type="entry name" value="Potassium voltage-gated channel protein Shaw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000047">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003974">
        <property type="entry name" value="K_chnl_volt-dep_Kv3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF252">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01498">
        <property type="entry name" value="SHAWCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="15">
        <location>
            <begin position="24"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="456"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="464"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015036102.2"/>
        </source>
    </evidence>
    <sequence length="627" mass="69079" checksum="69100CF11E662257" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8S1</accession>
    <name>A0A6I8V8S1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KappaPI-actitoxin-Avd3a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902977</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036111.2">
        <property type="nucleotide sequence ID" value="XM_015180625.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V8S1"/>
    <dbReference type="KEGG" id="dpo:6902977"/>
    <dbReference type="InParanoid" id="A0A6I8V8S1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V8S1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026200933" evidence="1">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="2">
        <location>
            <begin position="25"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036111.2"/>
        </source>
    </evidence>
    <sequence length="82" mass="9363" checksum="D62C799966C78C80" modified="2020-08-12" version="1">MKLIFVVFIIVALSGLSRAHKDPICGQKPAQHGYGDLFCAAAFPRFSYYPNLKECQKWLYSCGITNENHFKTKEECEAKCKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8W9</accession>
    <name>A0A6I8V8W9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cryptochrome-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">phr6-4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015036266.2">
        <property type="nucleotide sequence ID" value="XM_015180780.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V8W9"/>
    <dbReference type="FunCoup" id="A0A6I8V8W9">
        <property type="interactions" value="1037"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817210"/>
    <dbReference type="InParanoid" id="A0A6I8V8W9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.579.10:FF:000004">
        <property type="entry name" value="Cryptochrome-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF9">
        <property type="entry name" value="CRYPTOCHROME CIRCADIAN CLOCK 5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="8">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="500"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="500"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="258"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="299"/>
            <end position="306"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="397"/>
            <end position="399"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015036266.2"/>
        </source>
    </evidence>
    <sequence length="533" mass="61586" checksum="734D3AC501029EA8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9G3</accession>
    <name>A0A6I8V9G3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4816333</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015036606.2">
        <property type="nucleotide sequence ID" value="XM_015181120.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9G3"/>
    <dbReference type="FunCoup" id="A0A6I8V9G3">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V9G3"/>
    <dbReference type="UniPathway" id="UPA00163"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V9G3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045583">
        <property type="entry name" value="KPBA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF8">
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    </feature>
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        </location>
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        </location>
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        </location>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015037068.2"/>
        </source>
    </evidence>
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            <authorList>
                <consortium name="RefSeq"/>
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            <strain evidence="10 12">MV-25-SWS-2005</strain>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_033232933.1"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <source>
            <strain evidence="2">MV2-25</strain>
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            <authorList>
                <consortium name="RefSeq"/>
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        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
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    <dbReference type="KEGG" id="dpo:26532556"/>
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            <end position="144"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015037873.2"/>
        </source>
    </evidence>
    <sequence length="160" mass="18868" checksum="C76C55F03D526F2B" modified="2020-08-12" version="1">MTEKAIQYLIWQKSQVRRLENWASSSAMSWQRHLVVYVICRIAPSICRIRSWSYCCHCCCTMNAHSTAVSIEEDVGQGESRTPTEDVRSKLRQLQGHHLWLRHQLMLWQRHMLTERLSEQRDRHPRGSHKRNLGIPENINKPNVKVPTHQRSVTAMIDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VD91</accession>
    <name>A0A6I8VD91_DROPS</name>
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        <recommendedName>
            <fullName evidence="7">Erythrocyte membrane protein band 4.1-like 4A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein NBL4</fullName>
        </alternativeName>
    </protein>
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        <name evidence="11" type="primary">LOC6896886</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015038856.2">
        <property type="nucleotide sequence ID" value="XM_015183370.2"/>
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        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VD91"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13186">
        <property type="entry name" value="FERM_C_NBL4_NBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17107">
        <property type="entry name" value="FERM_F1_EPB41L4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000116">
        <property type="entry name" value="band 4.1-like protein 4A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR030696">
        <property type="entry name" value="Band4.1-like4A_FERM_F1"/>
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        <property type="entry name" value="Band_41_domain"/>
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        <property type="entry name" value="FA"/>
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    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
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    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
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        <property type="entry name" value="FERM_central"/>
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    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
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        <property type="entry name" value="FERM_N"/>
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    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF4">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 4A"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF08736">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
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    <dbReference type="Pfam" id="PF00373">
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    <dbReference type="Pfam" id="PF09379">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
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    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="9">
        <location>
            <begin position="11"/>
            <end position="292"/>
        </location>
    </feature>
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        <location>
            <begin position="314"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="405"/>
            <end position="704"/>
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    <feature type="region of interest" description="Disordered" evidence="8">
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        <location>
            <begin position="794"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="864"/>
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            <begin position="1483"/>
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            <begin position="316"/>
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            <begin position="408"/>
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    </feature>
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    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="775"/>
        </location>
    </feature>
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            <begin position="801"/>
            <end position="810"/>
        </location>
    </feature>
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            <end position="826"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
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        <location>
            <begin position="1485"/>
            <end position="1547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075138"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015038856.2"/>
        </source>
    </evidence>
    <sequence length="1602" mass="172197" checksum="D5DB5CDDD2B8CEDC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDB9</accession>
    <name>A0A6I8VDB9_DROPS</name>
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        <submittedName>
            <fullName evidence="9 10 11">Organic cation transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12" type="primary">LOC6897987</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9 10 11" key="2">
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="9 10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="nucleotide sequence ID" value="XM_015182912.2"/>
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        <property type="nucleotide sequence ID" value="XM_033376845.1"/>
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    <dbReference type="RefSeq" id="XP_033232737.1">
        <property type="nucleotide sequence ID" value="XM_033376846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDB9"/>
    <dbReference type="GeneID" id="6897987"/>
    <dbReference type="KEGG" id="dpo:6897987"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="Bgee" id="FBgn0247521">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="23"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="553"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="571"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002138086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015038398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033232736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033232737.1"/>
        </source>
    </evidence>
    <sequence length="584" mass="65613" checksum="03E6DA3CB7E672A1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDN7</accession>
    <name>A0A6I8VDN7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">CDGSH iron-sulfur domain-containing protein 3, mitochondrial isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">LOC4803406</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_015039064.1">
        <property type="nucleotide sequence ID" value="XM_015183578.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234941.1">
        <property type="nucleotide sequence ID" value="XM_033379050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDN7"/>
    <dbReference type="GeneID" id="4803406"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077452">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VDN7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.90">
        <property type="entry name" value="CDGSH iron-sulfur domain, mitoNEET-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052950">
        <property type="entry name" value="CISD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018967">
        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46491">
        <property type="entry name" value="CDGSH IRON SULFUR DOMAIN PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46491:SF3">
        <property type="entry name" value="CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09360">
        <property type="entry name" value="zf-CDGSH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron-binding zinc finger CDGSH type" evidence="6">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Iron-binding zinc finger CDGSH type" evidence="6">
        <location>
            <begin position="97"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015039064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033234941.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15712" checksum="B8A0FE9AEA96699E" modified="2020-08-12" version="1">MSIILKRSLRTPGLLKQVCYSSSSQSGDATAKTIPKNLVEDKQTAVLQKENGVIFDKRPFKIHLDKDKTYSWCLCGKSKSQPICDGTHKNEFLKIKLRPIRFKVEKSGDYWLCNCKQTSHRPFCDGTHKQPEIQAAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VF75</accession>
    <name>A0A6I8VF75_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4804483</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039976.1">
        <property type="nucleotide sequence ID" value="XM_015184490.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VF75"/>
    <dbReference type="GeneID" id="4804483"/>
    <dbReference type="InParanoid" id="A0A6I8VF75"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082038">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015039976.1"/>
        </source>
    </evidence>
    <sequence length="218" mass="23591" checksum="FA850958FC402022" modified="2020-08-12" version="1">MASTPARSVYITNEIIITAMDRFVWFVLLVVMSHLVTEFTRVSGYARGQRHELVLLNDTMLQPMPVQNMLLYPSHEYGQYQQRPSMNSLQQNAALLFSSLAAGAGAGAGTGASAEPSSNAAMLAVGDSQPAHNSEPSYPLFSLRPLIDSIFEIPISTLRAVNNMVGRLTGSYRQATAEVSQKSVAEGTALPLGHPSSNPTADSKFSVSQRRAMREEMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLV7</accession>
    <name>A0A6I8VLV7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Heparan sulfate 2-O-sulfotransferase pipe isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pip</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015044019.2">
        <property type="nucleotide sequence ID" value="XM_015188533.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLV7"/>
    <dbReference type="FunCoup" id="A0A6I8VLV7">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VLV7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001863">
        <property type="entry name" value="Heparan sulfate 2-o-sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF20">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE PIPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015044019.2"/>
        </source>
    </evidence>
    <sequence length="522" mass="61055" checksum="00857ADE466878B9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNE3</accession>
    <name>A0A6I8VNE3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Prolyl 4-hydroxylase subunit alpha-2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6896699</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232581.1">
        <property type="nucleotide sequence ID" value="XM_033376690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNE3"/>
    <dbReference type="KEGG" id="dpo:6896699"/>
    <dbReference type="InParanoid" id="A0A6I8VNE3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VNE3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Prolyl 4-hydroxylase N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232581.1"/>
        </source>
    </evidence>
    <sequence length="269" mass="30093" checksum="CCA87D6FF182CC8E" modified="2020-08-12" version="1">MEQPVGGAQMRSFDAWRDHLPTQLDLQDACNGIARIQDIYGLQEKHIMRGQLAGIKYNISMSTGDIFAMGQHLSRTTNAKEVVPWLEEVKRRLRNQTPGPLAVDKAEVLLLLAETYIKSKHYAEALPILDRALKHNIADAVLLRRREEVQQLIAKEPSTVLDNAEPKDNSKVAILKWGCRGFSREQSPKALHCCYNFTTTPFLRLAPPQNGATGGASLCRGLPRRPLRLGDSRDPRNGRAKDGQDIDRGATESDFVADPHSHGAWHRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNJ2</accession>
    <name>A0A6I8VNJ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sentrin-specific protease-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC117183309</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232645.1">
        <property type="nucleotide sequence ID" value="XM_033376754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNJ2"/>
    <dbReference type="KEGG" id="dpo:117183309"/>
    <dbReference type="InParanoid" id="A0A6I8VNJ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033232645.1"/>
        </source>
    </evidence>
    <sequence length="129" mass="14980" checksum="7C596FF642B246FF" modified="2020-08-12" version="1">MTTGMPRARKVLNTNRSRFRTPSAEMSRFPRGSPLKRWTSKIDVFSKDIILVPVHCNLNHWCMAIIHLKNKTIFYYDSLGDPSDIALDVLQNYIIAESLDKRKVQYDMSGFRIENVIECQRLVLHLALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNP1</accession>
    <name>A0A6I8VNP1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117183242</name>
        <name evidence="6" type="synonym">LOC117184871</name>
        <name evidence="7" type="synonym">LOC117184877</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232369.1">
        <property type="nucleotide sequence ID" value="XM_033376478.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239856.1">
        <property type="nucleotide sequence ID" value="XM_033383965.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239862.1">
        <property type="nucleotide sequence ID" value="XM_033383971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNP1"/>
    <dbReference type="KEGG" id="dpo:117183242"/>
    <dbReference type="KEGG" id="dpo:117184871"/>
    <dbReference type="KEGG" id="dpo:117184877"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="318"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033239856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033239862.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="41156" checksum="4E3F29F7EBD4CEED" modified="2020-08-12" version="1">MSDLVGTEEFSAAQLREWLESLNLPKGGSKAAMAARLNEIPVELRGQGPPAAETCENEREDEAAADQDSTKSERKEKNEKAPQAPGHNNNQGEYRAEVEMLKLQIELLKLQSEREKEGRGENTTPAANNDAGVMLLNAAKDMLPTYHGNISGNNDDVTTWIAQFKAVAKVNKLKDEKLLMLLMSKLKDKALVWLHSSPEHMSLPIDQLLNVMEDTFHPKESKLLLRRKFESRSWARGEEFSMYFNAKVSLASRIVIDDEEFIDGVIEGIPDVGLRRQAHMQCFGAPYQLLKAFEKIMLPKKYGSTEGANTGASPTPIRCYNCNSLGHVAGECRKPKRERGACYGCGSMSHQVSHCDEKKYKIASSTQGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPI1</accession>
    <name>A0A6I8VPI1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232778.1">
        <property type="nucleotide sequence ID" value="XM_033376887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPI1"/>
    <dbReference type="KEGG" id="dpo:117183225"/>
    <dbReference type="InParanoid" id="A0A6I8VPI1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033232778.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="14511" checksum="0E74F0C2ACBACC65" modified="2020-08-12" version="1">MDMKNIGNWHPNLRPNDFGGYRPGLQPLIYGEDMNRLVNYRNMKDFCEKLLQDQLFQAVVLAPVAELDSPPQNNFDLEDHKDRDYFVMLYDPACYHWPMQLKMLRKLSLLLANETISVINNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VRP3</accession>
    <name>A0A6I8VRP3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Longitudinals lacking protein, isoforms H/M/V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">rib</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361142.4">
        <property type="nucleotide sequence ID" value="XM_001361105.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233735.1">
        <property type="nucleotide sequence ID" value="XM_033377844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VRP3"/>
    <dbReference type="KEGG" id="dpo:4804609"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080192">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000019">
        <property type="entry name" value="Ligand-dependent corepressor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000148">
        <property type="entry name" value="Ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF105">
        <property type="entry name" value="RIBBON, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50960">
        <property type="entry name" value="HTH_PSQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="41"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="HTH psq-type" evidence="7">
        <location>
            <begin position="395"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="137"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="311"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="479"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="560"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="607"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="338"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="489"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="613"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="697"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361142.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033233735.1"/>
        </source>
    </evidence>
    <sequence length="712" mass="76549" checksum="2595950965127FB3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTR4</accession>
    <name>A0A6I8VTR4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Larval cuticle protein LCP-30 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Cpr49Ac</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234039.1">
        <property type="nucleotide sequence ID" value="XM_033378148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTR4"/>
    <dbReference type="FunCoup" id="A0A6I8VTR4">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532645"/>
    <dbReference type="InParanoid" id="A0A6I8VTR4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF200">
        <property type="entry name" value="CUTICULAR PROTEIN 49AB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026207218" evidence="3">
        <location>
            <begin position="24"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033234039.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="39450" checksum="80D37C4A462D15CF" modified="2020-08-12" version="1">MFTQSMLRFSAVVVVALIAVCHTSPVPDEDLNGKYTVSGQNSNAGKYRSVDDGRYHPSSQNEGRSGTQDSLGRYNHMPQPYTHVTDHRELGAYHHVPYPYDGGYGPYAGLNLPYVHDAKPYNHDLYTSTTTTTTKKPTTTTKRTTTTTTTTTTTPRNILYNYDDEGRHKILHQEEIRKKDKYDHAFLTENGIYGEEEAKIHHTGGTHAKGYYEYTGDDGMLYRVNYASNDGGFMPEGDHIPTPPPIPEAIARALKYVEEQHKANGGAQFDHRGFRINHMTKDIKAQIKSIHLERMPKELSDQIRMLEHEVELAEEEEREEERAEQERQKRKRLSDVHPTVQPVVPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYI3</accession>
    <name>A0A6I8VYI3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6902571</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235589.1">
        <property type="nucleotide sequence ID" value="XM_033379698.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902571"/>
    <dbReference type="InParanoid" id="A0A6I8VYI3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VYI3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000057">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026301829" evidence="8">
        <location>
            <begin position="23"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="9">
        <location>
            <begin position="123"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="9">
        <location>
            <begin position="739"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="9">
        <location>
            <begin position="1272"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="9">
        <location>
            <begin position="1888"/>
            <end position="1939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="484"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1099"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1176"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1632"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1715"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1798"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="493"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="654"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1109"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1185"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1642"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1720"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1803"/>
            <end position="1819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033235589.1"/>
        </source>
    </evidence>
    <sequence length="2245" mass="255531" checksum="B6565D0D9217BA0F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZY9</accession>
    <name>A0A6I8VZY9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SUN domain-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4817665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236647.1">
        <property type="nucleotide sequence ID" value="XM_033380756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZY9"/>
    <dbReference type="FunCoup" id="A0A6I8VZY9">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VZY9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VZY9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="206"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="376"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033236647.1"/>
        </source>
    </evidence>
    <sequence length="457" mass="53834" checksum="6DCFD2794D3566DA" modified="2020-08-12" version="1">MAKMGKWMTPEEEARQKFIMRERDRERKRIKRMNPEYRQMERERDRFRKQTPRPSLMTPEEEARHKFIMRERDRERKRIKRMNPEYRRMERERDRFRKKARRSNLTPEEELRLKMIQRERDRERKRIKRMNPEYRRLEQERDRDRKKARRANEAFRQLEKLRDKIRKDRKKGLLVTDPSQLPPEFAAILPPVPVVKPEVGLPPTSQGQQQQQQQQLQQQQQQQPPPSHLQEQQHHQLPHQRGRNMSHATSAASTLTQLTPPAHATPTHLQQLNKLQQLYPPRSFAHPALPITSVTLMPQLCHPILHQNLSATLYAGPNGIKQEYGQDVSASAMAAAQAAALASLRGQQHTQDDQDMALNLEPEIILQTAPDVNPAQPQQHHFTAHQQQQQQQQHHLQQQQQQQQHCNMFQHMAPQAHMQHMRSAALPPPPPSVALPPPPPPATHQQPPPQQLQHPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1M7</accession>
    <name>A0A6I8W1M7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC26532738</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polycystin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033237250.1">
        <property type="nucleotide sequence ID" value="XM_033381359.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532738"/>
    <dbReference type="InParanoid" id="A0A6I8W1M7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271725">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W1M7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002859">
        <property type="entry name" value="PKD/REJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014010">
        <property type="entry name" value="REJ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730:SF1">
        <property type="entry name" value="PLAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730">
        <property type="entry name" value="POLYCYSTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02010">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51111">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026261975" evidence="9">
        <location>
            <begin position="23"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1110"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1325"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1365"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="domain" description="REJ" evidence="11">
        <location>
            <begin position="160"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="10">
        <location>
            <begin position="1158"/>
            <end position="1275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033237250.1"/>
        </source>
    </evidence>
    <sequence length="1390" mass="161415" checksum="5C0DA223F65CE291" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W2X3</accession>
    <name>A0A6I8W2X3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3 4">Alsin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25" type="primary">LOC117183177</name>
    </gene>
    <organism evidence="1 24">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3 4">MV-25-SWS-2005</strain>
            <tissue evidence="2 3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237660.1">
        <property type="nucleotide sequence ID" value="XM_033381769.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237661.1">
        <property type="nucleotide sequence ID" value="XM_033381770.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237662.1">
        <property type="nucleotide sequence ID" value="XM_033381771.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237663.1">
        <property type="nucleotide sequence ID" value="XM_033381772.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237664.1">
        <property type="nucleotide sequence ID" value="XM_033381773.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237665.1">
        <property type="nucleotide sequence ID" value="XM_033381774.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237666.1">
        <property type="nucleotide sequence ID" value="XM_033381775.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033381776.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033381777.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033381778.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237670.1">
        <property type="nucleotide sequence ID" value="XM_033381779.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033381780.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237672.1">
        <property type="nucleotide sequence ID" value="XM_033381781.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033381783.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237675.1">
        <property type="nucleotide sequence ID" value="XM_033381784.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237676.1">
        <property type="nucleotide sequence ID" value="XM_033381785.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237677.1">
        <property type="nucleotide sequence ID" value="XM_033381786.1"/>
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    <dbReference type="RefSeq" id="XP_033237678.1">
        <property type="nucleotide sequence ID" value="XM_033381787.1"/>
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        <property type="nucleotide sequence ID" value="XM_033381789.1"/>
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        <property type="nucleotide sequence ID" value="XM_033381792.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8W2X3"/>
    <dbReference type="KEGG" id="dpo:117183177"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W2X3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Alsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46089">
        <property type="entry name" value="ALSIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46089:SF2">
        <property type="entry name" value="ALSIN HOMOLOG"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF25384">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033237660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033237661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033237662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033237663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033237664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033237665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033237666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033237667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033237668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033237669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033237670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033237671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033237672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033237673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033237674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033237675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033237676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033237677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033237678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_033237679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_033237680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_033237681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_033237682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_033237683.1"/>
        </source>
    </evidence>
    <sequence length="235" mass="25908" checksum="4917B59AC888D9B2" modified="2020-08-12" version="1">MCSAANDKAMKVAVTPGDDESFIIYHDGTPPLTRPPALRLCRIGDADRGGVLLLTTDHSLYSAQLQTNRSSLDFTLLRTDVVDVDYCSGSLELFVVLANGSVQRQFITGLGLDRDVHHPHAWQTLSFDPLEIIDEGVRIRRVCCSAQGVVFVSANGETYVMGSCGEVFKAELQPRHMRLYEEGKELLDLAAGNEHFVMLVAHYNLADDVLQLPLTIARRSPIPAGQNRTPCKIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4H3</accession>
    <name>A0A6I8W4H3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">REST corepressor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CoRest</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CoREST family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_033382364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4H3"/>
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        <property type="interactions" value="567"/>
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    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:transcription regulator complex"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="REST corepressor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049048">
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        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
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    </dbReference>
    <dbReference type="InterPro" id="IPR051066">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16089:SF28">
        <property type="entry name" value="REST COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16089">
        <property type="entry name" value="REST COREPRESSOR COREST PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20878">
        <property type="entry name" value="REST_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51156">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ELM2" evidence="10">
        <location>
            <begin position="88"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="11">
        <location>
            <begin position="174"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="11">
        <location>
            <begin position="509"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="240"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="400"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="570"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="682"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="570"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="625"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="686"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="738"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033238255.1"/>
        </source>
    </evidence>
    <sequence length="787" mass="84218" checksum="604394F9598C0422" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4V3</accession>
    <name>A0A6I8W4V3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">LOC4802059</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037576.2">
        <property type="nucleotide sequence ID" value="XM_015182090.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238388.1">
        <property type="nucleotide sequence ID" value="XM_033382497.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238395.1">
        <property type="nucleotide sequence ID" value="XM_033382504.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802059"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032584">
        <property type="term" value="C:growth cone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="7">
        <location>
            <begin position="435"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="812"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015037576.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033238388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238395.1"/>
        </source>
    </evidence>
    <sequence length="1366" mass="154122" checksum="7431B4A01D4F233A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W657</accession>
    <name>A0A6I8W657_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26534232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238833.1">
        <property type="nucleotide sequence ID" value="XM_033382942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W657"/>
    <dbReference type="KEGG" id="dpo:26534232"/>
    <dbReference type="InParanoid" id="A0A6I8W657"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238833.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="15406" checksum="B9FC4BE2ED78AEBE" modified="2020-08-12" version="1">MAERGQYPQVQRRPNATEALWEGAKQEAVDRELIPSIPKAKVLFPIALQGERALKLLQRQNPGVLESEEKPLMEASCYMAHDRPAPQDELGSLVTEAGPKNHQLIISTDSNAHQNVWGSPDIKDNGQADISQEEESKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W869</accession>
    <name>A0A6I8W869_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239568.1">
        <property type="nucleotide sequence ID" value="XM_033383677.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:26533215"/>
    <dbReference type="InParanoid" id="A0A6I8W869"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W869">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239568.1"/>
        </source>
    </evidence>
    <sequence length="107" mass="12844" checksum="4C669E5982EF093E" modified="2020-08-12" version="1">MRTVVRRGTDSDYIFEIHGRIRKQDRKRRKHFVLQRDKPHTKHIFATTSTLQQKQRASHYEVYKEEEDDDDDDDDDRPPDLTSFLSQPGVLAERYWELVVFIQNGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8E8</accession>
    <name>A0A6I8W8E8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TBC1 domain family member 9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Tbc1d8-9</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239651.1">
        <property type="nucleotide sequence ID" value="XM_033383760.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8W8E8">
        <property type="interactions" value="1305"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900879"/>
    <dbReference type="InParanoid" id="A0A6I8W8E8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003008">
        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13354">
        <property type="entry name" value="PH-GRAM2_TCB1D9_TCB1D9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000013">
        <property type="entry name" value="Putative TBC1 domain family member 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000002">
        <property type="entry name" value="TBC1 domain family member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000049">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000383">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
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</entry>
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    </dbReference>
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        </location>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WE50</accession>
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            <taxon>Diptera</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">MV2-25</strain>
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                <consortium name="RefSeq"/>
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            <strain evidence="6 7">MV-25-SWS-2005</strain>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="19">MV-25-SWS-2005</strain>
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    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="614"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033241179.1"/>
        </source>
    </evidence>
    <sequence length="1523" mass="164570" checksum="7BAFFBC235615951" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPL4</accession>
    <accession>A0A6I8V1M5</accession>
    <name>B5DPL4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BCL2/adenovirus E1B 19 kDa protein-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6900612</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134984.1">
        <property type="nucleotide sequence ID" value="XM_002134948.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPL4"/>
    <dbReference type="FunCoup" id="B5DPL4">
        <property type="interactions" value="776"/>
    </dbReference>
    <dbReference type="GeneID" id="6900612"/>
    <dbReference type="KEGG" id="dpo:6900612"/>
    <dbReference type="eggNOG" id="KOG3308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031907_0_0_1"/>
    <dbReference type="InParanoid" id="B5DPL4"/>
    <dbReference type="OMA" id="FWRKLIY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245189">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DPL4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000001">
        <property type="entry name" value="BCL2/adenovirus E1B protein-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022181">
        <property type="entry name" value="Bcl2-/adenovirus-E1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF22">
        <property type="entry name" value="MANGANESE-DEPENDENT INORGANIC PYROPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12496">
        <property type="entry name" value="BNIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002134984.1"/>
        </source>
    </evidence>
    <sequence length="465" mass="52316" checksum="ADE9C6E7E321C8B5" modified="2008-10-14" version="1">MSFHYAESESSQSESKMDISETPTDSTGISPLADECTQDTTPQVKTILEEEKDTKTSATLDNRIMRPELLSIETMPQRQIIERTLANSHPLMSPTNTDSDSEPFQTKRNNFPDVVPATEAAVQAKNTLNQQAPRNKPQDAKVSLLRTNSPDILSSESDADVSQYLAAVESNFQSVSLLPNSNGKKTKRGSTLPGGEDISSLDSISNHSFDDDEDIEHNLASLSPSSSLIDGLDGEDDDDECEGPELLPDNDDDLDLDFGLATTPTPHTPAAATLPQYSAAEERRDSRNWQKITLPDGRTREIDMRVIEPYKRVLSHGGYLKSGGQNAIVIFCACHLPDRSRADYSYVMDNLFLYVVKTLEQLVTDDYVLIYLHGGSNRRNVPPFPWLKRCYQLLDRRLRKSLKHMYLVHPTFWIKSLVWMARPFVSTKFWRKLIYVKSLEELGLHVAVEKAAIPEKVKQYDAKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>B5DU40</accession>
    <name>B5DU40_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein, isoform A</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein, isoform B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Dpse\GA23215</name>
        <name evidence="5" type="ORF">Dpse_GA23215</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
                <person name="Castrezana S."/>
                <person name="Celniker S.E."/>
                <person name="Chang J.L."/>
                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
                <person name="Clifton S.W."/>
                <person name="Comeron J.M."/>
                <person name="Costello J.C."/>
                <person name="Coyne J.A."/>
                <person name="Daub J."/>
                <person name="David R.G."/>
                <person name="Delcher A.L."/>
                <person name="Delehaunty K."/>
                <person name="Do C.B."/>
                <person name="Ebling H."/>
                <person name="Edwards K."/>
                <person name="Eickbush T."/>
                <person name="Evans J.D."/>
                <person name="Filipski A."/>
                <person name="Findeiss S."/>
                <person name="Freyhult E."/>
                <person name="Fulton L."/>
                <person name="Fulton R."/>
                <person name="Garcia A.C."/>
                <person name="Gardiner A."/>
                <person name="Garfield D.A."/>
                <person name="Garvin B.E."/>
                <person name="Gibson G."/>
                <person name="Gilbert D."/>
                <person name="Gnerre S."/>
                <person name="Godfrey J."/>
                <person name="Good R."/>
                <person name="Gotea V."/>
                <person name="Gravely B."/>
                <person name="Greenberg A.J."/>
                <person name="Griffiths-Jones S."/>
                <person name="Gross S."/>
                <person name="Guigo R."/>
                <person name="Gustafson E.A."/>
                <person name="Haerty W."/>
                <person name="Hahn M.W."/>
                <person name="Halligan D.L."/>
                <person name="Halpern A.L."/>
                <person name="Halter G.M."/>
                <person name="Han M.V."/>
                <person name="Heger A."/>
                <person name="Hillier L."/>
                <person name="Hinrichs A.S."/>
                <person name="Holmes I."/>
                <person name="Hoskins R.A."/>
                <person name="Hubisz M.J."/>
                <person name="Hultmark D."/>
                <person name="Huntley M.A."/>
                <person name="Jaffe D.B."/>
                <person name="Jagadeeshan S."/>
                <person name="Jeck W.R."/>
                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
                <person name="Li R."/>
                <person name="Lin C.F."/>
                <person name="Lin M.F."/>
                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
                <person name="Machado C.A."/>
                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
                <person name="O'Grady P."/>
                <person name="Pachter L."/>
                <person name="Papaceit M."/>
                <person name="Parisi M.J."/>
                <person name="Parisi M."/>
                <person name="Parts L."/>
                <person name="Pedersen J.S."/>
                <person name="Pesole G."/>
                <person name="Phillippy A.M."/>
                <person name="Ponting C.P."/>
                <person name="Pop M."/>
                <person name="Porcelli D."/>
                <person name="Powell J.R."/>
                <person name="Prohaska S."/>
                <person name="Pruitt K."/>
                <person name="Puig M."/>
                <person name="Quesneville H."/>
                <person name="Ram K.R."/>
                <person name="Rand D."/>
                <person name="Rasmussen M.D."/>
                <person name="Reed L.K."/>
                <person name="Reenan R."/>
                <person name="Reily A."/>
                <person name="Remington K.A."/>
                <person name="Rieger T.T."/>
                <person name="Ritchie M.G."/>
                <person name="Robin C."/>
                <person name="Rogers Y.H."/>
                <person name="Rohde C."/>
                <person name="Rozas J."/>
                <person name="Rubenfield M.J."/>
                <person name="Ruiz A."/>
                <person name="Russo S."/>
                <person name="Salzberg S.L."/>
                <person name="Sanchez-Gracia A."/>
                <person name="Saranga D.J."/>
                <person name="Sato H."/>
                <person name="Schaeffer S.W."/>
                <person name="Schatz M.C."/>
                <person name="Schlenke T."/>
                <person name="Schwartz R."/>
                <person name="Segarra C."/>
                <person name="Singh R.S."/>
                <person name="Sirot L."/>
                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
                <person name="Smith T.F."/>
                <person name="Spieth J."/>
                <person name="Stage D.E."/>
                <person name="Stark A."/>
                <person name="Stephan W."/>
                <person name="Strausberg R.L."/>
                <person name="Strempel S."/>
                <person name="Sturgill D."/>
                <person name="Sutton G."/>
                <person name="Sutton G.G."/>
                <person name="Tao W."/>
                <person name="Teichmann S."/>
                <person name="Tobari Y.N."/>
                <person name="Tomimura Y."/>
                <person name="Tsolas J.M."/>
                <person name="Valente V.L."/>
                <person name="Venter E."/>
                <person name="Venter J.C."/>
                <person name="Vicario S."/>
                <person name="Vieira F.G."/>
                <person name="Vilella A.J."/>
                <person name="Villasante A."/>
                <person name="Walenz B."/>
                <person name="Wang J."/>
                <person name="Wasserman M."/>
                <person name="Watts T."/>
                <person name="Wilson D."/>
                <person name="Wilson R.K."/>
                <person name="Wing R.A."/>
                <person name="Wolfner M.F."/>
                <person name="Wong A."/>
                <person name="Wong G.K."/>
                <person name="Wu C.I."/>
                <person name="Wu G."/>
                <person name="Yamamoto D."/>
                <person name="Yang H.P."/>
                <person name="Yang S.P."/>
                <person name="Yorke J.A."/>
                <person name="Yoshida K."/>
                <person name="Zdobnov E."/>
                <person name="Zhang P."/>
                <person name="Zhang Y."/>
                <person name="Zimin A.V."/>
                <person name="Baldwin J."/>
                <person name="Abdouelleil A."/>
                <person name="Abdulkadir J."/>
                <person name="Abebe A."/>
                <person name="Abera B."/>
                <person name="Abreu J."/>
                <person name="Acer S.C."/>
                <person name="Aftuck L."/>
                <person name="Alexander A."/>
                <person name="An P."/>
                <person name="Anderson E."/>
                <person name="Anderson S."/>
                <person name="Arachi H."/>
                <person name="Azer M."/>
                <person name="Bachantsang P."/>
                <person name="Barry A."/>
                <person name="Bayul T."/>
                <person name="Berlin A."/>
                <person name="Bessette D."/>
                <person name="Bloom T."/>
                <person name="Blye J."/>
                <person name="Boguslavskiy L."/>
                <person name="Bonnet C."/>
                <person name="Boukhgalter B."/>
                <person name="Bourzgui I."/>
                <person name="Brown A."/>
                <person name="Cahill P."/>
                <person name="Channer S."/>
                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
                <person name="Costello M."/>
                <person name="D'Aco K."/>
                <person name="Daza R."/>
                <person name="De Haan G."/>
                <person name="DeGray S."/>
                <person name="DeMaso C."/>
                <person name="Dhargay N."/>
                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
                <person name="Dorjee K."/>
                <person name="Dupes A."/>
                <person name="Elong R."/>
                <person name="Falk J."/>
                <person name="Farina A."/>
                <person name="Faro S."/>
                <person name="Ferguson D."/>
                <person name="Fisher S."/>
                <person name="Foley C.D."/>
                <person name="Franke A."/>
                <person name="Friedrich D."/>
                <person name="Gadbois L."/>
                <person name="Gearin G."/>
                <person name="Gearin C.R."/>
                <person name="Giannoukos G."/>
                <person name="Goode T."/>
                <person name="Graham J."/>
                <person name="Grandbois E."/>
                <person name="Grewal S."/>
                <person name="Gyaltsen K."/>
                <person name="Hafez N."/>
                <person name="Hagos B."/>
                <person name="Hall J."/>
                <person name="Henson C."/>
                <person name="Hollinger A."/>
                <person name="Honan T."/>
                <person name="Huard M.D."/>
                <person name="Hughes L."/>
                <person name="Hurhula B."/>
                <person name="Husby M.E."/>
                <person name="Kamat A."/>
                <person name="Kanga B."/>
                <person name="Kashin S."/>
                <person name="Khazanovich D."/>
                <person name="Kisner P."/>
                <person name="Lance K."/>
                <person name="Lara M."/>
                <person name="Lee W."/>
                <person name="Lennon N."/>
                <person name="Letendre F."/>
                <person name="LeVine R."/>
                <person name="Lipovsky A."/>
                <person name="Liu X."/>
                <person name="Liu J."/>
                <person name="Liu S."/>
                <person name="Lokyitsang T."/>
                <person name="Lokyitsang Y."/>
                <person name="Lubonja R."/>
                <person name="Lui A."/>
                <person name="MacDonald P."/>
                <person name="Magnisalis V."/>
                <person name="Maru K."/>
                <person name="Matthews C."/>
                <person name="McCusker W."/>
                <person name="McDonough S."/>
                <person name="Mehta T."/>
                <person name="Meldrim J."/>
                <person name="Meneus L."/>
                <person name="Mihai O."/>
                <person name="Mihalev A."/>
                <person name="Mihova T."/>
                <person name="Mittelman R."/>
                <person name="Mlenga V."/>
                <person name="Montmayeur A."/>
                <person name="Mulrain L."/>
                <person name="Navidi A."/>
                <person name="Naylor J."/>
                <person name="Negash T."/>
                <person name="Nguyen T."/>
                <person name="Nguyen N."/>
                <person name="Nicol R."/>
                <person name="Norbu C."/>
                <person name="Norbu N."/>
                <person name="Novod N."/>
                <person name="O'Neill B."/>
                <person name="Osman S."/>
                <person name="Markiewicz E."/>
                <person name="Oyono O.L."/>
                <person name="Patti C."/>
                <person name="Phunkhang P."/>
                <person name="Pierre F."/>
                <person name="Priest M."/>
                <person name="Raghuraman S."/>
                <person name="Rege F."/>
                <person name="Reyes R."/>
                <person name="Rise C."/>
                <person name="Rogov P."/>
                <person name="Ross K."/>
                <person name="Ryan E."/>
                <person name="Settipalli S."/>
                <person name="Shea T."/>
                <person name="Sherpa N."/>
                <person name="Shi L."/>
                <person name="Shih D."/>
                <person name="Sparrow T."/>
                <person name="Spaulding J."/>
                <person name="Stalker J."/>
                <person name="Stange-Thomann N."/>
                <person name="Stavropoulos S."/>
                <person name="Stone C."/>
                <person name="Strader C."/>
                <person name="Tesfaye S."/>
                <person name="Thomson T."/>
                <person name="Thoulutsang Y."/>
                <person name="Thoulutsang D."/>
                <person name="Topham K."/>
                <person name="Topping I."/>
                <person name="Tsamla T."/>
                <person name="Vassiliev H."/>
                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
                <person name="Weiand M."/>
                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
                <person name="Yu Q."/>
                <person name="Zembek L."/>
                <person name="Zhong D."/>
                <person name="Zimmer A."/>
                <person name="Zwirko Z."/>
                <person name="Jaffe D.B."/>
                <person name="Alvarez P."/>
                <person name="Brockman W."/>
                <person name="Butler J."/>
                <person name="Chin C."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Kleber M."/>
                <person name="Mauceli E."/>
                <person name="MacCallum I."/>
            </authorList>
            <dbReference type="PubMed" id="17994087"/>
            <dbReference type="DOI" id="10.1038/nature06341"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
            <authorList>
                <person name="English A.C."/>
                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CH672604">
        <property type="protein sequence ID" value="EDY71598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CH672604">
        <property type="protein sequence ID" value="KRT05614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DU40"/>
    <dbReference type="STRING" id="46245.B5DU40"/>
    <dbReference type="KEGG" id="dpo:6904114"/>
    <dbReference type="eggNOG" id="ENOG502QVSH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_550088_0_0_1"/>
    <dbReference type="OMA" id="KIRATHL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043596">
        <property type="entry name" value="CFAP53/TCHP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183:SF2">
        <property type="entry name" value="TRICHOPLEIN KERATIN FILAMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183">
        <property type="entry name" value="TRICHOPLEIN KERATIN FILAMENT-BINDING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="359"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EDY71598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KRT05614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <sequence length="501" mass="58875" checksum="E6ADCE5DD040496F" modified="2008-10-14" version="1">MPGRLNQQHAAYAQRRELENNRVQATQAVNRYYDHWGKVTTRFENWTNKEYYENAEKKLQTRKSQKQKDIDLTDRKGRLRQLLDTENEAYDIEMGRKRRPRECTADTQMLGRVNQSLKEQEQLKRKLEMEAKLYGRWRHGVDDEKFLYQSKSDNEVLAKLNWLDKQIEQQKERDEQEALNAERKLELQQEISRTELAQKERQLIQEQQIKEIRELQESHMVELRQHQSQAEKLKEEEQHFRLFIGELEKEKQLLEESTALVLQRPDTGQAFNLNKIKVFIRNRSEASRRQISLCINLLERMAKYVVRTEDLQSLLHKYKEQLESEQMAYTQIEAMYESEAKSSLQRCEENWREQHLQRYQELSKLIDTEKDCLDRLLQENVSQQQALLELRSTHLSGIEQATKKLEQITSGEQGSASSDPISVSSSTDLCAGALAEEDDASVNVPKVSNSFTNLNLDVWQDMPPVRGGINSPRLDKTRSLNVVTAQTALPPKFARKRVAWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I7LZD3</accession>
    <accession>A0A6I8UK01</accession>
    <name>I7LZD3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cleavage and polyadenylation specificity factor subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Clp</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356742.2">
        <property type="nucleotide sequence ID" value="XM_001356706.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7LZD3"/>
    <dbReference type="SMR" id="I7LZD3"/>
    <dbReference type="FunCoup" id="I7LZD3">
        <property type="interactions" value="1219"/>
    </dbReference>
    <dbReference type="STRING" id="46245.I7LZD3"/>
    <dbReference type="GeneID" id="4817272"/>
    <dbReference type="KEGG" id="dpo:4817272"/>
    <dbReference type="eggNOG" id="KOG1040">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024513_0_1_1"/>
    <dbReference type="InParanoid" id="I7LZD3"/>
    <dbReference type="OMA" id="FHQNNFA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="Bgee" id="FBgn0077593">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000005">
        <property type="entry name" value="cleavage and polyadenylation specificity factor subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000019">
        <property type="entry name" value="cleavage and polyadenylation specificity factor subunit 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
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    </dbReference>
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        <property type="entry name" value="CPSF4/Yth1"/>
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    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102:SF24">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18345">
        <property type="entry name" value="zf_CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001356742.2"/>
        </source>
    </evidence>
    <sequence length="282" mass="32121" checksum="B8CD0E4F02DD3EA7" modified="2012-10-03" version="1">MDILLANVNSLQFKAERDLIEQVGAIPLPFYGMDKSIAAVCNFITRSGQECDKGSACPFRHIRGDRTIVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSRFNACHNKECPFLHIDPQSKVKDCPWYKRGFCRHGPHCRHQHLRRVLCMNYLAGFCPEGPNCKHMHPRFELPPLADLSKDQLHKKLPTCHYCGELGHKANSCKQYAGSLENRNNINAMDHSGGDGNDDGPLSHPPPPFLKIPTPLEEITCYKCGHKGHYANKCPKGHLAFLSSARTHRSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q29AA9</accession>
    <name>HH_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein hedgehog</fullName>
            <ecNumber evidence="3">3.1.-.-</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">Protein hedgehog N-product</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">hh-1</name>
        <name type="ORF">GA18321</name>
    </gene>
    <gene>
        <name type="primary">hh-2</name>
        <name type="ORF">GA29124</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <molecule>Protein hedgehog</molecule>
        <text evidence="1 3">The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product) (By similarity). In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity). Once cleaved, the C-product has no signaling activity and diffuses from the cell.</text>
    </comment>
    <comment type="function">
        <molecule>Protein hedgehog N-product</molecule>
        <text evidence="1">The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development. Establishes the anterior-posterior axis of the embryonic segments and patterns the larval imaginal disks. Binds to the patched (ptc) receptor, which functions in association with smoothened (smo), to activate the transcription of target genes wingless (wg), decapentaplegic (dpp) and ptc. In the absence of hh, ptc represses the constitutive signaling activity of smo through fused (fu). Essential component of a signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required to activate Cad99C-dependent endosome formation, norpA-dependent Ca2+ mobilization and p38 MAPK, which are essential steps in the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection. During photoreceptor differentiation, it up-regulates transcription of Ubr3, which in turn promotes the hh-signaling pathway by mediating the ubiquitination and degradation of cos.</text>
    </comment>
    <comment type="catalytic activity">
        <molecule>Protein hedgehog</molecule>
        <reaction evidence="3">
            <text>glycyl-L-cysteinyl-[protein] + cholesterol + H(+) = [protein]-C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:59504"/>
            <dbReference type="Rhea" id="RHEA-COMP:12707"/>
            <dbReference type="Rhea" id="RHEA-COMP:15369"/>
            <dbReference type="Rhea" id="RHEA-COMP:15374"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:65250"/>
            <dbReference type="ChEBI" id="CHEBI:143135"/>
            <dbReference type="ChEBI" id="CHEBI:143140"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:59505"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with shf.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Nuclear up to embryonic stage 10 and then at stage 11 shifts to the cytoplasm. Also secreted in either cleaved or uncleaved form to mediate signaling to other cells.</text>
    </comment>
    <comment type="subcellular location">
        <molecule>Protein hedgehog N-product</molecule>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <text evidence="1">The N-terminal peptide remains associated with the cell surface. Heparan sulfate proteoglycans of the extracellular matrix play an essential role in diffusion. Lipophorin is required for diffusion, probably by acting as vehicle for its movement, explaining how it can spread over long distances despite its lipidation.</text>
    </comment>
    <comment type="PTM">
        <molecule>Protein hedgehog</molecule>
        <text evidence="1 2 3">The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product) (By similarity). In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (By similarity). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.</text>
    </comment>
    <comment type="PTM">
        <molecule>Protein hedgehog N-product</molecule>
        <text evidence="3">Cholesterylation is required for N-product targeting to lipid rafts and multimerization.</text>
    </comment>
    <comment type="PTM">
        <molecule>Protein hedgehog N-product</molecule>
        <text evidence="1">N-palmitoylation by Rasp of the hedgehog N-product, within the secretory pathway, is required for the embryonic and larval patterning activities of the hedgehog signal.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the hedgehog family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CH676084">
        <property type="protein sequence ID" value="EDY71847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL27441.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29AA9"/>
    <dbReference type="SMR" id="Q29AA9"/>
    <dbReference type="FunCoup" id="Q29AA9">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29AA9"/>
    <dbReference type="MEROPS" id="C46.001"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0283937">
        <property type="protein sequence ID" value="FBpp0282375"/>
        <property type="gene ID" value="FBgn0078326"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0289638">
        <property type="protein sequence ID" value="FBpp0288076"/>
        <property type="gene ID" value="FBgn0250482"/>
    </dbReference>
    <dbReference type="GeneID" id="4801169"/>
    <dbReference type="KEGG" id="dpo:4801169"/>
    <dbReference type="CTD" id="42737"/>
    <dbReference type="eggNOG" id="KOG3638">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034686_0_0_1"/>
    <dbReference type="InParanoid" id="Q29AA9"/>
    <dbReference type="OMA" id="HWVSSLL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078326">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140853">
        <property type="term" value="F:cholesterol-protein transferase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016015">
        <property type="term" value="F:morphogen activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005113">
        <property type="term" value="F:patched binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016540">
        <property type="term" value="P:protein autoprocessing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007367">
        <property type="term" value="P:segment polarity determination"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097264">
        <property type="term" value="P:self proteolysis"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.16.10:FF:000001">
        <property type="entry name" value="Indian hedgehog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.10:FF:000001">
        <property type="entry name" value="Indian hedgehog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001657">
        <property type="entry name" value="Hedgehog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001767">
        <property type="entry name" value="Hedgehog_Hint"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050387">
        <property type="entry name" value="Hedgehog_Signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000320">
        <property type="entry name" value="Hedgehog_signalling_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Zn_M74/Hedgehog-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11889">
        <property type="entry name" value="HEDGEHOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11889:SF31">
        <property type="entry name" value="PROTEIN HEDGEHOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01085">
        <property type="entry name" value="HH_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01079">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009400">
        <property type="entry name" value="Peptidase_C46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00632">
        <property type="entry name" value="SONICHHOG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="Hedgehog/DD-peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0106">Calcium</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0504">Morphogen</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-0564">Palmitate</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0709">Segmentation polarity protein</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end status="unknown"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000383070" evidence="4">
        <location>
            <begin status="unknown"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000383071" description="Protein hedgehog" evidence="1">
        <location>
            <begin position="93"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000383072" description="Protein hedgehog N-product" evidence="1">
        <location>
            <begin position="93"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="1">
        <location>
            <begin position="265"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="site" description="Involved in cholesterol transfer" evidence="1">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="site" description="Involved in auto-cleavage" evidence="1">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="site" description="Essential for auto-cleavage" evidence="1">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="N-palmitoyl cysteine" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="Cholesterol glycine ester" evidence="1">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q02936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q15465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q62226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="EAL27441.2"/>
        </source>
    </evidence>
    <sequence length="481" mass="53485" checksum="1F21C76E2B87BE1C" modified="2008-10-14" version="2" precursor="true">MDNHNEVPMSMSVPWASAASVTCLSLDAKCHRPCPSSISASASASACASDSAAIATTKLRHIAYTQRCSSRLTMLMTVLLLLLPLSFTPAHSCGPGRGLGRRRERNLYPLVLKQTIPNLSEYQTGASGPLEGEIKRDSPKFKDLVPNYNRDILFRDEEGTGADRLMTKRCKEKLNVLAYSVMNEWPGVRLLVTESWDEDHQHGQESLHYEGRAVTIATSDREPSRYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSSISSHVHGCFTPESTALLESGITKPLSEISIGDRVLSMGSNGQPVYSEVILFMDRNLEQMQNFVELHTDGGAVLTVTPAHLISVWHPERQQLDYVFADRVEELNYVLVRDPQTGELRPQRVVRVGSVRSKGVVAPLTREGTIVVNSVAASCYAVIDSQSLAHWGLAPMRILAMLQSWMPAKDQLRSSQTEGVVSRAEQQNGIHWYANALYKVKDYVLPKSWRHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q29F47</accession>
    <accession>A0A6I8UED6</accession>
    <name>Q29F47_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354173.3">
        <property type="nucleotide sequence ID" value="XM_001354137.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29F47"/>
    <dbReference type="STRING" id="46245.Q29F47"/>
    <dbReference type="GeneID" id="4813997"/>
    <dbReference type="KEGG" id="dpo:4813997"/>
    <dbReference type="eggNOG" id="ENOG502SGND">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010718_6_1_1"/>
    <dbReference type="InParanoid" id="Q29F47"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079025">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF47">
        <property type="entry name" value="GH22833P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="202"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354173.3"/>
        </source>
    </evidence>
    <sequence length="500" mass="56422" checksum="1F57B5611DF5C8FD" modified="2016-01-20" version="3">MSCLSRSQENAAAAETEFGYLFRQQLESHRKQPLPINQSSEMDTMAVAVPPAAGPSNGGGMAMAVTMGMGMDISVINQYEENVEHLRQLFSQNHLVSFRIDHIACSAGSSSGDNYMSVVKRVTISQDQEQPITSEAVTVIVKRQIPSLSRRQLYRCEEAFSNEINAYRHLVPLLRRYSQQPLFPLCHIAESHDQSETGEPIIVLQDLKALGYRMKDRLDGLELEHCLLVMKKLAQMHAASLAAQQLESALFAAQVEHMSEIVYCEDAAEFYSTILDTSVQQALDSLSSSNADGSLTTPISLIEGLRPKLFEQLQRNINAAAESPFSVVCHGDLWVNNIMFRSEPDEVIFFDLQAMRRTSPVFDILHFIYTSTRRRLRDDHTDTLLAAYAEAVAKELNQQLKHTPAAKQLDQLSDTFSLQRLTTEYVRQVHYGLAIGMWILPAVTFDPNNLPNLDVMSEQNLTGKEIKCTQTLTSEYHLRIRELVMEFYERGYLQTQRPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GA4</accession>
    <accession>A0A6I8UGL0</accession>
    <name>Q29GA4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein CheA7a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CheA7a</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355149.2">
        <property type="nucleotide sequence ID" value="XM_001355113.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GA4"/>
    <dbReference type="FunCoup" id="Q29GA4">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GA4"/>
    <dbReference type="GeneID" id="4815259"/>
    <dbReference type="KEGG" id="dpo:4815259"/>
    <dbReference type="eggNOG" id="ENOG502T0SD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1541348_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GA4"/>
    <dbReference type="OMA" id="KPFCQFI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073485">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21112">
        <property type="entry name" value="CHEMOSENSORY PROTEIN A 29A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21112:SF13">
        <property type="entry name" value="CHEMOSENSORY PROTEIN A 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355149.2"/>
        </source>
    </evidence>
    <sequence length="180" mass="20671" checksum="3CF068534F972F5D" modified="2008-10-14" version="2">MKRFCSLWPFALLLAIHVCRAEKPYNVELNSFDQNKDYETDGKWVDWGTLRMKKVSRNKFALAGDFEFKLNMGDEQGIMLRVFVYDSNAGQKGPLVMNVKKPFCQFINEDEDTYPYLQKSSNMPEQGDCPFPKGQYKIDNYELETEFLPDDAPKGDYLLELTLLDKDLGVAGLIAIVTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29H23</accession>
    <accession>A0A6I8UFX7</accession>
    <name>Q29H23_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Tim8</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354880.2">
        <property type="nucleotide sequence ID" value="XM_001354844.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29H23"/>
    <dbReference type="FunCoup" id="Q29H23">
        <property type="interactions" value="1620"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29H23"/>
    <dbReference type="GeneID" id="4814627"/>
    <dbReference type="KEGG" id="dpo:4814627"/>
    <dbReference type="eggNOG" id="KOG3489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141397_1_2_1"/>
    <dbReference type="InParanoid" id="Q29H23"/>
    <dbReference type="OMA" id="NEICWDK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074464">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="2">
        <location>
            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354880.2"/>
        </source>
    </evidence>
    <sequence length="86" mass="9974" checksum="03AE508EFC52507B" modified="2008-10-14" version="2">MSDFETDLTSNDKELQEFLMMEKQKAQVNAQIHEFNEICWEKCIGKPSAKLDSATETCLSNCVDRFIDTSLLITQRFAQILQKHDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JT8</accession>
    <accession>A0A6I8UHX8</accession>
    <name>Q29JT8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Trs23</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Core component of the TRAPP complexes which has a function of guanine nucleotide exchange factor activity for Rab1 GTPase. Plays a role in vesicular transport from endoplasmic reticulum to Golgi and autophagy. May play a role in dendrite postsynaptic membrane trafficking.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12. Interacts with SDC2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
            <location evidence="9">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355814.1">
        <property type="nucleotide sequence ID" value="XM_001355778.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JT8"/>
    <dbReference type="SMR" id="Q29JT8"/>
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        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JT8"/>
    <dbReference type="GeneID" id="4816104"/>
    <dbReference type="KEGG" id="dpo:4816104"/>
    <dbReference type="eggNOG" id="KOG3369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053380_1_0_1"/>
    <dbReference type="InParanoid" id="Q29JT8"/>
    <dbReference type="OMA" id="GQRDGIN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081665">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JT8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.40:FF:000001">
        <property type="entry name" value="Trafficking protein particle complex 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000009">
        <property type="entry name" value="Trafficking protein particle complex 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001355814.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="24630" checksum="90E5120669091C5B" modified="2006-04-04" version="1">MIIYGVYIVSKSGGLIFNLDNHVPRIEHEKTFTYPLDLVLDYDSKKVSVAFNRKDGINVGHVLVAVNGIPVNGITLDDGRDVKTTLEAAENYPINLKFSRPKTTTNEKIFLASMFYPLFAIASQLSPEPKSSGIELLEADTFTLHCFQTLTGIKFIVISETGLNGIDLLLRKVYELYSDYVLKNPFYSLEMPIRCELFDNKLQELLAQVEKTGISNIDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-06-15" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KG4</accession>
    <name>AFFL_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AF4/FMR2 family member lilli</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Protein lilliputian</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lilli</name>
        <name type="ORF">GA21343</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has a role in transcriptional regulation. Acts in parallel with the Ras/MAPK and the PI3K/PKB pathways in the control of cell identity and cellular growth. Essential for regulation of the cytoskeleton and cell growth but not for cell proliferation or growth rate. Required specifically for the microtubule-based basal transport of lipid droplets. Plays a partially redundant function downstream of Raf in cell fate specification in the developing eye. Pair-rule protein that regulates embryonic cellularization, gastrulation and segmentation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379061">
        <property type="protein sequence ID" value="EAL33211.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KG4"/>
    <dbReference type="SMR" id="Q29KG4"/>
    <dbReference type="FunCoup" id="Q29KG4">
        <property type="interactions" value="266"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KG4"/>
    <dbReference type="eggNOG" id="ENOG502QR32">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_241798_0_0_1"/>
    <dbReference type="InParanoid" id="Q29KG4"/>
    <dbReference type="OMA" id="NMEATWT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032783">
        <property type="term" value="C:super elongation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007366">
        <property type="term" value="P:periodic partitioning by pair rule gene"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032368">
        <property type="term" value="P:regulation of lipid transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007797">
        <property type="entry name" value="AF4/FMR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043640">
        <property type="entry name" value="AF4/FMR2_CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000637">
        <property type="entry name" value="HMGI/Y_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528">
        <property type="entry name" value="AF4/FMR2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528:SF17">
        <property type="entry name" value="AF4_FMR2 FAMILY MEMBER LILLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18876">
        <property type="entry name" value="AFF4_CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00354">
        <property type="entry name" value="HMGI_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-0562">Pair-rule protein</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000394677" description="AF4/FMR2 family member lilli">
        <location>
            <begin position="1"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="A.T hook" evidence="2">
        <location>
            <begin position="900"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="134"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="402"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="761"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="822"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1291"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1483"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1656"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="154"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="227"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="448"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="463"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="483"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
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            <begin position="511"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="521"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="582"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="615"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="626"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="647"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="768"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="844"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1483"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1656"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="863"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="864"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="920"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="922"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="1442"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9VQI9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="EAL33211.2"/>
        </source>
    </evidence>
    <sequence length="1770" mass="188886" checksum="59A03A81699B7F68" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29L21</accession>
    <accession>A0A6I8UIC4</accession>
    <name>Q29L21_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I and III subunit RPAC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Polr1C</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355944.1">
        <property type="nucleotide sequence ID" value="XM_001355908.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29L21"/>
    <dbReference type="FunCoup" id="Q29L21">
        <property type="interactions" value="1002"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29L21"/>
    <dbReference type="GeneID" id="4816522"/>
    <dbReference type="KEGG" id="dpo:4816522"/>
    <dbReference type="eggNOG" id="KOG1521">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_0_1_1"/>
    <dbReference type="InParanoid" id="Q29L21"/>
    <dbReference type="OMA" id="KKKCRAF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077675">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29L21">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07032">
        <property type="entry name" value="RNAP_I_II_AC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000003">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033901">
        <property type="entry name" value="RNAPI/III_AC40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF13">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="7">
        <location>
            <begin position="52"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355944.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="38263" checksum="399115E3FA5C95AD" modified="2006-04-04" version="1">MSSPIRKEPLLYMEEHRVKEDPQDYGLADDVFNMEVFKKKLQVAIVRNDEDGLEFDMIGVYPAIANAFRRLMLSEVPSMAIEKVYIYNNTSIIQDEVLAHRMGLLPLKADPRLFAYRTEESGEAGTEQDTLEYELKVKCTRRKDASKDQSNFDDIYKNHKVYSGHLKWLPKGKQAQIYSESMVNCIHEDILIAQLRPGHELDLRLVAVKGLGKDHAKFSPVATAYYRLLPQIKLNREVYGKDAYLLQSCFSPGVIGIDENECAYVKDARYDSCSRNVYRYPQLEDSVTLGRVRDHYIFTVESVGSLKPDVIFLEAVKVLKKKCRAFIDEIEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LF3</accession>
    <accession>A0A6I8UKN2</accession>
    <name>Q29LF3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">pseudouridine 5'-phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pseudouridine-5'-monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4817630</name>
        <name evidence="11" type="synonym">LOC6903193</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>psi-UMP + H2O = pseudouridine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10944"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17802"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58380"/>
            <dbReference type="EC" id="3.1.3.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.96" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001357026.1">
        <property type="nucleotide sequence ID" value="XM_001356990.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002132892.1">
        <property type="nucleotide sequence ID" value="XM_002132856.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LF3"/>
    <dbReference type="SMR" id="Q29LF3"/>
    <dbReference type="STRING" id="46245.Q29LF3"/>
    <dbReference type="GeneID" id="4817630"/>
    <dbReference type="GeneID" id="6903193"/>
    <dbReference type="KEGG" id="dpo:4817630"/>
    <dbReference type="KEGG" id="dpo:6903193"/>
    <dbReference type="eggNOG" id="KOG2914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_0_1"/>
    <dbReference type="OMA" id="THCIFDN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078973">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990738">
        <property type="term" value="F:pseudouridine 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000055">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002132892.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="26669" checksum="06C7963D4351BD50" modified="2006-04-04" version="1">MASRACYKPVTHIIFDSDGTVLDTENMYSDVINEIVAPFGKSYTYEMKLRYMGMPALQSARQLVEELQLPLTPVEYLKIFDSVVFGRINNVQLLPGVKDLMLHLHDHRIPMAIATSSVQAAFHTKSQPHRDLFPALHHVVCGDDPELRPGRGKPHPDIFLLAASRFHPAPDPGQCLVFEDSPNGLQAGIAAGMQVVMIPDPRVPSEQRTGATQVLDSMADFEPQLFGLPHYDTCSKFEFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MR3</accession>
    <accession>A0A6I8UJM0</accession>
    <name>Q29MR3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase UBR7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4817112</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356567.1">
        <property type="nucleotide sequence ID" value="XM_001356531.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MR3"/>
    <dbReference type="FunCoup" id="Q29MR3">
        <property type="interactions" value="2531"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MR3"/>
    <dbReference type="GeneID" id="4817112"/>
    <dbReference type="KEGG" id="dpo:4817112"/>
    <dbReference type="eggNOG" id="KOG2752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025221_0_0_1"/>
    <dbReference type="InParanoid" id="Q29MR3"/>
    <dbReference type="OMA" id="GAMVYNH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073559">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MR3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15542">
        <property type="entry name" value="PHD_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19677">
        <property type="entry name" value="UBR-box_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040204">
        <property type="entry name" value="UBR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047506">
        <property type="entry name" value="UBR7-like_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="228"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001356567.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="45013" checksum="9027F352C8BB0683" modified="2006-04-04" version="1">MSDTNATTTDAEGEEVNTLEQSTITMVDVLEQEKEMEDEYAAVLGASDEKACTYAKGPIQRQALYSCLTCCPEARTDLAKCAGVCLACSYRCHENHELVELYTKRNFRCDCPTLRLGAVKRCCLNPQLEGPQPSNDGNLYNQNFQGLYCQCKRPYPDPERTTEEVMLQCAVCEDWYHLPHMKAPGASEKMLDSCSEMICDTCMDKNQFLRDYTALALQAVEAEESKADLKADKVQVDAEADSKPEQTEEPASKRVKLSTGDCRRPNPTKKHQGAAFWTNDWRKSLCKCTECLALFKEFAVEFLLDADDSAKAYEERGMKRAEENSSYEQGMRALSSIDRTQQIDAITEYNRMTDKLKDYLKGFALRDKVVTPEDINVFFTRMRDENKPSVGQPHFCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MS4</accession>
    <accession>A0A6I8UJV7</accession>
    <name>Q29MS4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine/arginine-rich splicing factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SC35</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356555.2">
        <property type="nucleotide sequence ID" value="XM_001356519.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MS4"/>
    <dbReference type="FunCoup" id="Q29MS4">
        <property type="interactions" value="2370"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MS4"/>
    <dbReference type="GeneID" id="4816924"/>
    <dbReference type="KEGG" id="dpo:4816924"/>
    <dbReference type="eggNOG" id="KOG4207">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_10_3_1"/>
    <dbReference type="InParanoid" id="Q29MS4"/>
    <dbReference type="OMA" id="PDRANMS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078884">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12311">
        <property type="entry name" value="RRM_SRSF2_SRSF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001092">
        <property type="entry name" value="serine/arginine-rich splicing factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="100"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356555.2"/>
        </source>
    </evidence>
    <sequence length="207" mass="22285" checksum="CB1DA057CAEE1B07" modified="2008-10-14" version="2">MSNGGSAGGLGGTRPPPRIDGMVSLKVDNLTYRTTPEDLRRVFERCGEVGDIYIPRDRYTRESRGFAFVRFYDKRDAEDALEAMDGRMLDGRELRVQMARYGRPSSPTRSNGRRGGGSGGGGGGGGAGGGGGGGGGRRRSRSRSPMRRRSRSPRRRSYSPRSRSQSAASHSPERRTKFSRSPVRGESRNGIGASALAVGASRSRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NJ6</accession>
    <accession>A0A6I8UL76</accession>
    <name>Q29NJ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357156.1">
        <property type="nucleotide sequence ID" value="XM_001357120.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NJ6"/>
    <dbReference type="FunCoup" id="Q29NJ6">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NJ6"/>
    <dbReference type="GeneID" id="4817798"/>
    <dbReference type="KEGG" id="dpo:4817798"/>
    <dbReference type="eggNOG" id="KOG4319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180450_1_1_1"/>
    <dbReference type="InParanoid" id="Q29NJ6"/>
    <dbReference type="OMA" id="EAFFDEY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079848">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29NJ6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018792">
        <property type="entry name" value="NUPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149">
        <property type="entry name" value="NUCLEAR PROTEIN 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149:SF4">
        <property type="entry name" value="RH17958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10195">
        <property type="entry name" value="Phospho_p8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357156.1"/>
        </source>
    </evidence>
    <sequence length="68" mass="7938" checksum="8E9DAB234F62C5F2" modified="2006-04-04" version="1">MSESHFDEYEHYNFDYDKHIFSGHGGKQRSKKEASVHTNHFDPSGHSRKILTKLMNTNTNNIKKACKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29P35</accession>
    <accession>A0A6I8ULG5</accession>
    <name>Q29P35_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Ras-related protein Rab-30</fullName>
            <ecNumber evidence="8">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC4818089</name>
        <name evidence="26 27" type="synonym">Rab30</name>
    </gene>
    <organism evidence="26">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26 27">MV-25-SWS-2005</strain>
            <strain evidence="26">MV2-25</strain>
            <tissue evidence="26 27">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="24">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB30 is required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins. Facilitates lipid homeostasis during fasting by regulating hepatic protein and lipid trafficking in a PPAR-alpha-dependent manner. Promotes autophagosome biogenesis during bacterial infection such as group A Streptococcus infection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
            <location evidence="6">Autophagosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="8"/>
    <dbReference type="RefSeq" id="XP_001357389.2">
        <property type="nucleotide sequence ID" value="XM_001357353.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035469.1">
        <property type="nucleotide sequence ID" value="XM_015179983.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29P35"/>
    <dbReference type="SMR" id="Q29P35"/>
    <dbReference type="STRING" id="46245.Q29P35"/>
    <dbReference type="GeneID" id="4818089"/>
    <dbReference type="KEGG" id="dpo:4818089"/>
    <dbReference type="eggNOG" id="KOG0095">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="OMA" id="VYDVSCQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081528">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29P35">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04114">
        <property type="entry name" value="Rab30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000440">
        <property type="entry name" value="Ras-related protein Rab-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041820">
        <property type="entry name" value="Rab30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0636">Prenylation</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00067826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00093384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_001357389.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_015035469.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="24703" checksum="860218E7C08A1CAC" modified="2008-10-14" version="2">MEDYKFLFKIVLVGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEVEGEKIKLQIWDTAGQERFRSITQSYYRSAHALILVYDISCQPTFDCLPDWLREIQEYANSKVLKILVGNKTDRDDREIPTQIGEEFAKQHDMYFLETSAKEAENVERLFYEIAAELIGQARSKDGSSAAAAAAAQRQSDGSSIGLASLGAKAAQSNCCGGLANAGGGGSGGGANTSQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M009</accession>
    <accession>A0A6I8UD71</accession>
    <name>Q2M009_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4813572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353614.2">
        <property type="nucleotide sequence ID" value="XM_001353578.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M009"/>
    <dbReference type="FunCoup" id="Q2M009">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="GeneID" id="4813572"/>
    <dbReference type="KEGG" id="dpo:4813572"/>
    <dbReference type="HOGENOM" id="CLU_150248_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M009"/>
    <dbReference type="OMA" id="RTKTRNN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080280">
        <property type="expression patterns" value="Expressed in male reproductive system"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003475">
        <property type="entry name" value="Insect_Unk"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02448">
        <property type="entry name" value="L71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353614.2"/>
        </source>
    </evidence>
    <sequence length="100" mass="11527" checksum="E28F82BC2E8E0F69" modified="2008-10-14" version="2">MNINIVLALFVVLCLTLISGQRDDCEALRRACETCSSSLNNPSDRNLPTLNAECRRKTRNTWIWRNVNRCELTRLNCIGSNRRMNCADIAELARMQRVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB40</accession>
    <name>A0A6I8UB40_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RINT1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Rint1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RINT1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352684.3">
        <property type="nucleotide sequence ID" value="XM_001352648.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UB40"/>
    <dbReference type="FunCoup" id="A0A6I8UB40">
        <property type="interactions" value="2799"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812604"/>
    <dbReference type="InParanoid" id="A0A6I8UB40"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060628">
        <property type="term" value="P:regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.670:FF:000003">
        <property type="entry name" value="RAD50-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520:SF0">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1 RINT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352684.3"/>
        </source>
    </evidence>
    <sequence length="733" mass="85246" checksum="E742705ADF97DCA6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBL3</accession>
    <name>A0A6I8UBL3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4812544</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATA6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352927.3">
        <property type="nucleotide sequence ID" value="XM_001352891.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBL3"/>
    <dbReference type="KEGG" id="dpo:4812544"/>
    <dbReference type="InParanoid" id="A0A6I8UBL3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBL3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120212">
        <property type="term" value="C:sperm head-tail coupling apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032027">
        <property type="term" value="F:myosin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042769">
        <property type="entry name" value="SPATA6_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032732">
        <property type="entry name" value="SPATA6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435:SF6">
        <property type="entry name" value="IP09370P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 6 SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14909">
        <property type="entry name" value="SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 6 N-terminal" evidence="4">
        <location>
            <begin position="10"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352927.3"/>
        </source>
    </evidence>
    <sequence length="277" mass="31346" checksum="B7EF3A429E64BEA7" modified="2020-08-12" version="1">MSHKRFYVRLELQLHALTCPGVWLCSHGYLEATIKTLGYYFRTGAMEPRFPMLCHDQFKMEGYFKSVGSLEEMHELLRAEQLQITIWQNGRRLAYFLGSLSDVMQPTVPRLSCAHSSNVQLLMKATPAFPGILAPKVELSAQMTTQDRQKGCSCSSLNSRRRPKEDWRPPVNRHVESRCTSRVEHPRKQQTVCHAKKSNCDHGACSYSGSASPEQQQQQNRRRLSTCSSSTQISSVSQSSSLTHGSHSFCCSSSDPADDHESSCDICQAYRRIFSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFT1</accession>
    <name>A0A6I8UFT1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AH receptor-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4814582</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354809.3">
        <property type="nucleotide sequence ID" value="XM_001354773.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFT1"/>
    <dbReference type="FunCoup" id="A0A6I8UFT1">
        <property type="interactions" value="441"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814582"/>
    <dbReference type="InParanoid" id="A0A6I8UFT1"/>
    <dbReference type="OMA" id="SHCCGMM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074972">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFT1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000052">
        <property type="entry name" value="Aryl-hydrocarbon-interacting protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039663">
        <property type="entry name" value="AIP/AIPL1/TTC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056277">
        <property type="entry name" value="PPIase_AIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242:SF0">
        <property type="entry name" value="TPR_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23322">
        <property type="entry name" value="PPIase_AIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="AIP/AIPL N-terminal FKBP-type PPIase" evidence="5">
        <location>
            <begin position="7"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001354809.3"/>
        </source>
    </evidence>
    <sequence length="320" mass="37255" checksum="7B426D6AC40B0B3A" modified="2020-08-12" version="1">MEHKKNVENKPIRKEIINPGNAYIELTPGTRVKFHFQTRTAHDARIVDDSRKMDKPMELVLGKKFKLEVWEVIVQQMSLNEVAKFTVHKSLCPQYPFISKTLRDIGKKPEERRRCCGMTLQNEGIGYPDLDALLQHPVDLEFIIELISIELPEQYEKERWQMSDDEKMRATNTLRERGNGFYKSNRYTDAETCYREAVGIVEQLMLKEKPHDGEWKELAAIKTPLLLNYAQCRLIAGDFYAVIEHCNEVLNLDPRNVKALFRRAKAHAGAWNPMQARRDFIDALALDSSLKSTVAKELKSIEEQQQARNVQDRIHMQKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGN9</accession>
    <name>A0A6I8UGN9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dystrotelin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dah</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355193.4">
        <property type="nucleotide sequence ID" value="XM_001355157.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGN9"/>
    <dbReference type="FunCoup" id="A0A6I8UGN9">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815230"/>
    <dbReference type="InParanoid" id="A0A6I8UGN9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGN9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046716">
        <property type="term" value="P:muscle cell cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02345">
        <property type="entry name" value="ZZ_dah"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF21">
        <property type="entry name" value="DISCONTINUOUS ACTIN HEXAGON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="7">
        <location>
            <begin position="283"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="362"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="377"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355193.4"/>
        </source>
    </evidence>
    <sequence length="661" mass="73692" checksum="79DF05EB2F5E12B7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHS0</accession>
    <name>A0A6I8UHS0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">trypsin</fullName>
            <ecNumber evidence="10">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4815758</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001355601.3">
        <property type="nucleotide sequence ID" value="XM_001355565.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHS0"/>
    <dbReference type="FunCoup" id="A0A6I8UHS0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815758"/>
    <dbReference type="InParanoid" id="A0A6I8UHS0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHS0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 14" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026171441" description="trypsin" evidence="11">
        <location>
            <begin position="21"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="14"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355601.3"/>
        </source>
    </evidence>
    <sequence length="249" mass="25663" checksum="C3157DD5B2D0230F" modified="2020-08-12" version="1">MHSVQTLLVLLLAVAMGVQASRLPADVGSHPYAISLQRNGAHIAGGALISQRWALTAAHCVTVGGGQESYPARTFLVRAGSIQRLVGGQLVALSRIVVHSDYSSGGAGANDLALLELASPLTLNANTQPIGLASERPAVGSQITFAGWGSSQPDGALSHGLQVATRQSISADACQESLFLSQQDLLCLVPEADDVESSGLCTGDAGSPAVYNNQLVGIAAFFVSSCGTEQPDGYVDVTQHLDWIAENSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJE6</accession>
    <name>A0A6I8UJE6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="21 22">Tyrosine-protein phosphatase cdc-14 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">Cdc14</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21 22">MV-25-SWS-2005</strain>
            <tissue evidence="21 22">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
            <location evidence="14">Kinocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356478.4">
        <property type="nucleotide sequence ID" value="XM_001356442.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235815.1">
        <property type="nucleotide sequence ID" value="XM_033379924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJE6"/>
    <dbReference type="KEGG" id="dpo:4817482"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060091">
        <property type="term" value="C:kinocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050877">
        <property type="term" value="P:nervous system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14499">
        <property type="entry name" value="CDC14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17657">
        <property type="entry name" value="CDC14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000032">
        <property type="entry name" value="dual specificity protein phosphatase CDC14A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000006">
        <property type="entry name" value="Dual specificity protein phosphatase CDC14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044506">
        <property type="entry name" value="CDC14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029260">
        <property type="entry name" value="DSPn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14671">
        <property type="entry name" value="DSPn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="184"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="267"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="653"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="709"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="854"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="979"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="653"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="713"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="873"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="979"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1024"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1067"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001356478.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_033235815.1"/>
        </source>
    </evidence>
    <sequence length="1091" mass="119309" checksum="4E2D1C6EA6CCF480" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJJ5</accession>
    <name>A0A6I8UJJ5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="2">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA pseudouridine 55 synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Pus10</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001356481.3">
        <property type="nucleotide sequence ID" value="XM_001356445.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJJ5"/>
    <dbReference type="FunCoup" id="A0A6I8UJJ5">
        <property type="interactions" value="2016"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817266"/>
    <dbReference type="InParanoid" id="A0A6I8UJJ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJJ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2510:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3190:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048742">
        <property type="entry name" value="Pus10_N_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21237">
        <property type="entry name" value="Pus10_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pus10 N-terminal eukaryotes" evidence="8">
        <location>
            <begin position="72"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Pus10-like C-terminal" evidence="9">
        <location>
            <begin position="252"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00079393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00083669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001356481.3"/>
        </source>
    </evidence>
    <sequence length="498" mass="56509" checksum="151E4CDACBB59D3B" modified="2020-08-12" version="1">MKNQELVDYLMSCGVCKVCQLRYLRARGSEYNDIHKSLQRLGVKVTEDQVEDEDNVISTDEQPLKKPRLSHCSTCLGIFSEDFQRELIEGIIASEFGKYDSEGIVLAITMPMSLQLRQLSMWFALQRKFGSTVHSNSPPDVPIKEAVKLILHPIICKKLDKKYDANGLVISIDLRHKVEEEEVDKLVELNRAAFPGKALRRVEISRGLLEKQYQPSRVKAETFEKYLPIPPTAVDESLSLKSVDLQGPMVCVAGRYRKLSRELSHTPWLLQGVRQMEDSIEEIIIRSVGPHFTETVPKITFMSSGREDVDVRCLGKGRPFVLEIPNSRRSTLTREQALAMEQAVDTSGKVSILNLQVVPREQAMNIKTGEEQKRKYYRALCALKEPATLEMLQKLQISESFDIQQKTPIRVLHRRPLHTRPRTIYSVKARLQRDNPLVLVIDIVTQAGTYIKELVHGEFGRTTPSFSSIIGKPIDIQALDVVGIDLDWPPEVDNSVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK87</accession>
    <name>A0A6I8UK87_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Geranylgeranyl transferase type-1 subunit beta</fullName>
            <ecNumber evidence="4">2.5.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Geranylgeranyl transferase type I subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Type I protein geranyl-geranyltransferase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">betaggt-I</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterodimer of FNTA and PGGT1B. PGGT1B mediates interaction with substrate peptides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.59" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001356723.4">
        <property type="nucleotide sequence ID" value="XM_001356687.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK87"/>
    <dbReference type="FunCoup" id="A0A6I8UK87">
        <property type="interactions" value="262"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817123"/>
    <dbReference type="InParanoid" id="A0A6I8UK87"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK87">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02895">
        <property type="entry name" value="GGTase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000005">
        <property type="entry name" value="Geranylgeranyl transferase type-1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041960">
        <property type="entry name" value="GGTase_I_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF4">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-1 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="16">
        <location>
            <begin position="14"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001356723.4"/>
        </source>
    </evidence>
    <sequence length="399" mass="44448" checksum="DD2BCDFE67959305" modified="2020-08-12" version="1">MEDNSTEPEPVLLSKHAKNLLRFLNMLPARMASHDNTRSTIVFFAVCGLDVLNSLNLVPPKMRQDIIDWIYGGLVVPRDNEKRCGGFMGCRAMVPLTENSEVLECMRKYQWGHLAMTYTSIAVLVTLGDDLSRLDRKSIVDGVAAVQRPEGSFSACIDGSEDDMRFVYCAATICHMLDYWGDVDKEAMFQFIEKSLRYDYGFSQELEGEAHGGTTFCALAALQLSGQLDRLHAATLERIKRWLIFRQVDGFQGRPNKPVDTCYSFWIGASLCILNGFELTDYAKNREYILSTQDTLVGGFAKWPQATPDPFHTYLGLCGLAFTGEPGLSAVMPSLNMSMAAYVHLQHLHEVWRSKSNEGKEDHDLTLSSAVQQQLKLVEDGEAKATTATTSTSPLISAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK99</accession>
    <name>A0A6I8UK99_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polycomb protein esc</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein extra sex combs</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">escl</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat ESC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356837.4">
        <property type="nucleotide sequence ID" value="XM_001356801.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK99"/>
    <dbReference type="FunCoup" id="A0A6I8UK99">
        <property type="interactions" value="1764"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817087"/>
    <dbReference type="InParanoid" id="A0A6I8UK99"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK99">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000056">
        <property type="entry name" value="Polycomb protein eed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051243">
        <property type="entry name" value="PcG_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10253">
        <property type="entry name" value="POLYCOMB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="272"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="318"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="69"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001356837.4"/>
        </source>
    </evidence>
    <sequence length="527" mass="59670" checksum="40B2C3834F513D0D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULQ4</accession>
    <name>A0A6I8ULQ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Luciferin sulfotransferase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4800360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357554.3">
        <property type="nucleotide sequence ID" value="XM_001357517.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULQ4"/>
    <dbReference type="FunCoup" id="A0A6I8ULQ4">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800360"/>
    <dbReference type="InParanoid" id="A0A6I8ULQ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULQ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="55"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357554.3"/>
        </source>
    </evidence>
    <sequence length="316" mass="37064" checksum="D0707AEF23EDB7CE" modified="2020-08-12" version="1">MQLVYRELDADIVRRTNAVFPAQNCFVEVLPDQLIIPRKYVELGESIRSLPVYEDDVWMISYPRTGSTWAQEMVWLLGHELDYVAAEQDIRMRSPLLELSALFSTDHHEMVAKAFGNTVELVRNLPRPRYARSHLSWQLLPEQFDTVKPRIVYTARNPKDLCVSYYHYCKLLHGISGDFEQFVELFLGGHTPMGSYWKHVLPFWKRSNDENVLFIKYEDMITDLPAVVRRCGRFLNATHLLNETGMKRICEHLTFDKMRQNKAVNLEKVLPASSTKFIRNGKIGDWRNHMSEEMSERFDAWSEQHVRGSGLRFDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMJ3</accession>
    <name>A0A6I8UMJ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Septin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Septin2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357795.1">
        <property type="nucleotide sequence ID" value="XM_001357758.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMJ3"/>
    <dbReference type="SMR" id="A0A6I8UMJ3"/>
    <dbReference type="FunCoup" id="A0A6I8UMJ3">
        <property type="interactions" value="464"/>
    </dbReference>
    <dbReference type="GeneID" id="4800531"/>
    <dbReference type="KEGG" id="dpo:4800531"/>
    <dbReference type="InParanoid" id="A0A6I8UMJ3"/>
    <dbReference type="OMA" id="NNGIHIY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078015">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000036">
        <property type="entry name" value="septin-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="40"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="344"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001357795.1"/>
        </source>
    </evidence>
    <sequence length="419" mass="48521" checksum="BB69068578634A57" modified="2020-08-12" version="1">MTAEVDFVNKKEMHLRTLKQSGHVGFDSLPDQLVNKSVQNGFVFNVMCIGETGLGKSTLMDTLFNTSFESTPSPHTLPSVKLKAHTYELQESNVRLKLTICDTVGYGDQINKDDSFKAVVDYIDAQFENYLQEELKIKRSLVTCHDSRIHICLYFICPTGHGLKSLDLVCMKKLDSKVNIIPVIAKADTISKVELQRFKAKIIQELNTNGVHIYQFPTDDETVAETNTSMNSHIPFAVVGSTEFIKVGNKLIRARQYPWGTVQVENETHCDFVKLREMLIRTNMEDMREKTHTRHYELYRQKRLEQMGFSDVDSENKPISFQQTFEAKRSNHLAELQSKEEEVRQMFVQRVKEKEAELKESEKDLHAKFEKLKRDHAEEKRKLEESRKALEEDYLDFQRRKQQLASAHHTLTLGKSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNX1</accession>
    <name>A0A6I8UNX1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein Osi22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Osi22</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358400.2">
        <property type="nucleotide sequence ID" value="XM_001358363.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNX1"/>
    <dbReference type="FunCoup" id="A0A6I8UNX1">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801280"/>
    <dbReference type="InParanoid" id="A0A6I8UNX1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026047023" evidence="3">
        <location>
            <begin position="21"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358400.2"/>
        </source>
    </evidence>
    <sequence length="342" mass="37731" checksum="68ABB4F6AE848008" modified="2020-08-12" version="1">MSSLVRFLLLSVLCTALTRGHDGGQETTSEAASATEAARGLASSYEPEDKQSLRKNSHIFMGIYKNYKSTYLGNKTTSEYKKRLRDRVGASQIANNEAAEGPEPQSDQEQLEAADVLAQELRQEANAEALLEAQTGTPIYDDNETSAGKKRRKRKRKDRNKRREESGAAETGTATGTEQPDMQSEDETIQRYFVGPGLNVSLDMSNDIVHVKLDGENLKEIMAARWLTTDNSEEGRGKKYDMITKVLPLFILPFLIQSAIVPFLVTKLKLLLVKSILVGKLAIFLLIISAIKNGNKMVQSYEVPSYWAGEPSRRSELAAAASSAAAAYNGYRVEGKPTTWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ96</accession>
    <name>A0A6I8UQ96_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Enhancer of split m7 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">E(spl)m7-HLH</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359173.2">
        <property type="nucleotide sequence ID" value="XM_001359136.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ96"/>
    <dbReference type="FunCoup" id="A0A6I8UQ96">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="GeneID" id="4802206"/>
    <dbReference type="KEGG" id="dpo:4802206"/>
    <dbReference type="InParanoid" id="A0A6I8UQ96"/>
    <dbReference type="OMA" id="NNEYHRV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19741">
        <property type="entry name" value="bHLH-O_ESMB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="13"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="7">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359173.2"/>
        </source>
    </evidence>
    <sequence length="206" mass="22476" checksum="8DA0335E35B5683B" modified="2020-08-12" version="1">MATKYEVSKTYQYRKVMKPLLERRRRARINMCLDELKDLMVQCTVQSGDGKFDRADILEVTVDHLRKLKQARIEATAAAAKATTNTTPEQSFRDGFIRAADEVSRALASLPNVDVVFGTHLMTHLGLRLNQLETPTAAPKPMNAPLSIICGSGGSSSSSSNCSSNSREACSPVSSGYCSDSDCSVSSTQAAQNLLQISTGQVWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQG8</accession>
    <name>A0A6I8UQG8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transmembrane protease serine 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4802383</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359312.1">
        <property type="nucleotide sequence ID" value="XM_001359275.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQG8"/>
    <dbReference type="FunCoup" id="A0A6I8UQG8">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="GeneID" id="4802383"/>
    <dbReference type="KEGG" id="dpo:4802383"/>
    <dbReference type="InParanoid" id="A0A6I8UQG8"/>
    <dbReference type="OMA" id="VINEHWI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080128">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF18">
        <property type="entry name" value="OVOCHYMASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026030286" evidence="2">
        <location>
            <begin position="28"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="82"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359312.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="36826" checksum="720A2607BADAA2CD" modified="2020-08-12" version="1">MLLSYRCIYSVLLTTIASIMVVLSSSASQSSLLPTVRKCGGANSAMGSSAHTMAMNLAAYGLLENRISTLEAPRQTHWSKKFLAKREATPHTAPYVVSIQMMTPDQGLVHYCAGTVINEHWILTAAHCLNSPQTVENSVIVAGSHDIHDHRGEAANIQMRHIDYYVRHELYLGGVNPYDIALIYIKRPLVFDEYVQPAMLPEQDAHPEGYGTLYGWGNVSMTAVPNYPHKLQEANMPILDMELCEQILARSGLQLHETNLCTGPLTGGVSICTADSGGPLIQQCCEDSFEQGNIVIGIVSWGKMPCGQKNAPSVFVRVSAFTDWIHQIISSATHIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQU2</accession>
    <name>A0A6I8UQU2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="31">ATP-binding cassette sub-family B member 6</fullName>
            <ecNumber evidence="30">7.6.2.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">ABC-type heme transporter ABCB6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="46 47" type="primary">Hmt-1</name>
    </gene>
    <organism evidence="45 46">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="45" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="45">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="46 47" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="46 47">MV-25-SWS-2005</strain>
            <strain evidence="45">MV2-25</strain>
            <tissue evidence="46 47">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>coproporphyrin I(in) + ATP + H2O = coproporphyrin I(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66768"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167478"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:66769"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>coproporphyrin III(in) + ATP + H2O = coproporphyrin III(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:131725"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:66665"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>coproporphyrinogen III(in) + ATP + H2O = coproporphyrinogen III(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:66681"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>heme b(in) + ATP + H2O = heme b(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19261"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:19262"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>pheophorbide a(in) + ATP + H2O = pheophorbide a(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61360"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58687"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:61361"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>protoporphyrin IX(in) + ATP + H2O = protoporphyrin IX(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61336"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:61337"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>uroporphyrin I(in) + ATP + H2O = uroporphyrin I(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66772"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167480"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:66773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>uroporphyrin III(in) + ATP + H2O = uroporphyrin III(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66776"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167479"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:66777"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
            <location evidence="2">Multivesicular body membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Melanosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion outer membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="29">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.5" evidence="30"/>
    <dbReference type="RefSeq" id="XP_001359262.3">
        <property type="nucleotide sequence ID" value="XM_001359225.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238276.1">
        <property type="nucleotide sequence ID" value="XM_033382385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQU2"/>
    <dbReference type="KEGG" id="dpo:4802322"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015439">
        <property type="term" value="F:ABC-type heme transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18581">
        <property type="entry name" value="ABC_6TM_ABCB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03253">
        <property type="entry name" value="ABCC_ATM1_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000022">
        <property type="entry name" value="ATP-binding cassette sub-family B member 6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032410">
        <property type="entry name" value="ABCB6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF654">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16185">
        <property type="entry name" value="MTABC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20 46 47" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="26" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0967">Endosome</keyword>
    <keyword evidence="23" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="27" id="KW-0458">Lysosome</keyword>
    <keyword evidence="25 42" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0964">Secreted</keyword>
    <keyword evidence="21" id="KW-1278">Translocase</keyword>
    <keyword evidence="15 42" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="22 42" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="44">
        <location>
            <begin position="251"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="43">
        <location>
            <begin position="575"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="41">
        <location>
            <begin position="820"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="41">
        <location>
            <begin position="820"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="41">
        <location>
            <begin position="843"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="41">
        <location>
            <begin position="862"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00024320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00024385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00024439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00031413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00047649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00047753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00047789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00048309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00048455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00048510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00048636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00049398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="43">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="44">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="46">
        <source>
            <dbReference type="RefSeq" id="XP_001359262.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="47">
        <source>
            <dbReference type="RefSeq" id="XP_033238276.1"/>
        </source>
    </evidence>
    <sequence length="871" mass="98684" checksum="44A1515E3F9A70AA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR55</accession>
    <name>A0A6I8UR55_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359486.3">
        <property type="nucleotide sequence ID" value="XM_001359449.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR55"/>
    <dbReference type="FunCoup" id="A0A6I8UR55">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802595"/>
    <dbReference type="InParanoid" id="A0A6I8UR55"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="12"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359486.3"/>
        </source>
    </evidence>
    <sequence length="192" mass="21514" checksum="9A93831D9C9E6AD3" modified="2020-08-12" version="1">MANAVVNTTSPMAGRRLQRDVTRLLASDHRATVDDNMSSLAVLFEGPLGTAYEGGMWTVNISMPKDYPLNPPKVRFITKILHPNIEFNTGLVCMNVFSQAWSPSYDLLNIIETFLPQLLRNPNPNDPLNHHAAQIMKHSEAMFRQNVIACVNMYARPTPMCRLSEAGKQLLEQRSSDLSLSELLSDDDVDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URV5</accession>
    <name>A0A6I8URV5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NACHT and WD repeat domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4802886</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359714.3">
        <property type="nucleotide sequence ID" value="XM_001359677.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8URV5">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802886"/>
    <dbReference type="InParanoid" id="A0A6I8URV5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.370:FF:000003">
        <property type="entry name" value="Leucine-rich repeat and WD repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001000">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001701">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056534">
        <property type="entry name" value="Beta-prop_NWD2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025139">
        <property type="entry name" value="DUF4062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052752">
        <property type="entry name" value="NACHT-WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057588">
        <property type="entry name" value="NWD1/2-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871">
        <property type="entry name" value="BETA TRANSDUCIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871:SF14">
        <property type="entry name" value="DUF4062 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23586">
        <property type="entry name" value="Beta-prop_NWD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13271">
        <property type="entry name" value="DUF4062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25469">
        <property type="entry name" value="WHD_NWD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF4062" evidence="4">
        <location>
            <begin position="26"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="NWD1/2-like winged helix-turn-helix" evidence="6">
        <location>
            <begin position="611"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1274"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="domain" description="NWD2 C-terminal beta-propeller" evidence="5">
        <location>
            <begin position="1295"/>
            <end position="1650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359714.3"/>
        </source>
    </evidence>
    <sequence length="1726" mass="195552" checksum="BC4E1B7B6E253CDF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USF3</accession>
    <name>A0A6I8USF3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dephospho-CoA kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Dpck</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoaE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360054.3">
        <property type="nucleotide sequence ID" value="XM_001360017.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USF3"/>
    <dbReference type="SMR" id="A0A6I8USF3"/>
    <dbReference type="FunCoup" id="A0A6I8USF3">
        <property type="interactions" value="863"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803289"/>
    <dbReference type="InParanoid" id="A0A6I8USF3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000485">
        <property type="entry name" value="Dephospho-CoA kinase CAB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360054.3"/>
        </source>
    </evidence>
    <sequence length="233" mass="26708" checksum="E667760BFEC92B2A" modified="2020-08-12" version="1">MFIVAVTGGIATGKSTVSKVFERHGIPVIDADKIAREIVEPGQAAWEQIRATFGEDVLLPSKEINRAVLARMIFEDKELRGKLNQITHPKIHRKIFWRAFKLFVTGHSWIVLDLPLLFETGILMDFIYKIVCVSCDSDKQLQRLIARNELSEAEASHRIESQMPLEKKCEQSHFVIDNNGSIEETEESAIRVVNMMKESKQHWRNRVYLLGLVMIVGFTIYMLLKIFGRVPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USR3</accession>
    <name>A0A6I8USR3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Circadian clock-controlled protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4803301</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360064.2">
        <property type="nucleotide sequence ID" value="XM_001360027.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USR3"/>
    <dbReference type="FunCoup" id="A0A6I8USR3">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="GeneID" id="4803301"/>
    <dbReference type="KEGG" id="dpo:4803301"/>
    <dbReference type="InParanoid" id="A0A6I8USR3"/>
    <dbReference type="OMA" id="WIQWLEN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080081">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000001">
        <property type="entry name" value="Takeout-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF32">
        <property type="entry name" value="CIRCADIAN CLOCK-CONTROLLED PROTEIN DAYWAKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026066642" evidence="4">
        <location>
            <begin position="21"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360064.2"/>
        </source>
    </evidence>
    <sequence length="256" mass="30558" checksum="7E8B3335FABA692D" modified="2020-08-12" version="1">MLYLPFIFWITLELFVGLQCQTFPDKVNKCHYGDGKCLAESANALIRNYPKGIPEINLKPFDVVTVKDWVLVNDSRVGGAWYYFQLFNQVNLGFENTTITEIKGFDRDPTKSKIEIHGKIPRLVYKGKYHAKGRMLWFVDINSKGDSSSDFLNFRFDLTLKVRTEYRNNKRYMKIYEVVPNLRLDRWIMWMDDFFPDNFDMTIAINNLFNRNWVEFWNELEPRILLLFETVFLSLFEDLFYNIPYDDLFLADSEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT54</accession>
    <name>A0A6I8UT54_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor YJU2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4803871</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA. Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the spliceosome. Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CWC16 family. YJU2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360520.3">
        <property type="nucleotide sequence ID" value="XM_001360483.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT54"/>
    <dbReference type="SMR" id="A0A6I8UT54"/>
    <dbReference type="FunCoup" id="A0A6I8UT54">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803871"/>
    <dbReference type="InParanoid" id="A0A6I8UT54"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03226">
        <property type="entry name" value="YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043701">
        <property type="entry name" value="Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF1">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="108"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001360520.3"/>
        </source>
    </evidence>
    <sequence length="319" mass="36405" checksum="7BAF339C5739C627" modified="2020-08-12" version="1">MSERKVLNKYYPPDFDPSKIPRMKLAKNRQYTVRLMAPFNMRCVTCGEYIYKGKKFNARKEDVEHETYLGIRIYRFYIKCTRCLQEISFKTDPQNTDYEIEAGATRNFMALKLAEEQARRELQEEREEEANNPMKMLENRTEQSRNEIEMLESLEELRDLNRRQQTVDYTTMLQQYNTGETERERLEREEREDEEFIKSVNFKSKPEGGTRIVAEEIIEEVKEEAVDLLPAPPPAKLAKPICPLPAAASTSKVLAAQPLVKRKTPLVLVKPKAAATTKPTATSTTATDIPKAAEVPKTPAPAAGLSLLAAYSDSSEDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTE7</accession>
    <name>A0A6I8UTE7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">SAM50-like protein CG7639</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4804017</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360647.2">
        <property type="nucleotide sequence ID" value="XM_001360610.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTE7"/>
    <dbReference type="FunCoup" id="A0A6I8UTE7">
        <property type="interactions" value="1765"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804017"/>
    <dbReference type="InParanoid" id="A0A6I8UTE7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033108">
        <property type="term" value="P:mitochondrial respiratory chain complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.50:FF:000002">
        <property type="entry name" value="sorting and assembly machinery component 50 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="POTRA" evidence="8">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360647.2"/>
        </source>
    </evidence>
    <sequence length="445" mass="49193" checksum="B4358A61DC602447" modified="2020-08-12" version="1">MPQSKPRGGAASSKDSKYDLGKISARVDRVHVSGLLRTHNDYVMRAADGLFKADNFQDVMLEAMSTKAYLHELGIFKDVSVHIDVSRGADASPQGYEVTFKGNEYSRMMGSAGTEVGQNEGSLRTELTVPNILGRGESISLQGSYSNTRANDLQLKFWKPFFHTRFRENRPEFSFSIFRQTDRFDISSFQSTNVGYLIDFSAHSSLGVDLTHSLQYENSIRNVGLLNKSVPFGIREHCGPKLASLVRYSVVFDNRDANVFATRGILLKSTNEYCGLGGNVAYTSSTAHGEVNVPLFSGFVAQFCGRVGIVKETKQTTLLPISSLFYCGGPMTLRGFKYGGAGPVVDGTPIGAQTYWCTGAHLWAPLPFAGVFKSLASNFRTHFFYNIGNTNTFSLENMRSAFGMGLAFKFADRARIEINYCIPVRRQATDKVLNGFQFGIGYEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUH9</accession>
    <name>A0A6I8UUH9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial inner membrane protease subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">IMP2-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4804426</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of 2 subunits, IMMPL1 and IMMPL2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S26 family. IMP2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360992.2">
        <property type="nucleotide sequence ID" value="XM_001360955.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUH9"/>
    <dbReference type="FunCoup" id="A0A6I8UUH9">
        <property type="interactions" value="632"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804426"/>
    <dbReference type="InParanoid" id="A0A6I8UUH9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000005">
        <property type="entry name" value="Mitochondrial inner membrane protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 16" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase S26" evidence="14">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S26" evidence="14">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001360992.2"/>
        </source>
    </evidence>
    <sequence length="169" mass="18756" checksum="CAB91979D6A4CD60" modified="2020-08-12" version="1">MAFRFFAKSLLYGLPLGITFFDCVGYVARVDGTSMQPALNPVADVRDYVFLLRWGNHNSDVERGDIISLVSPKDPGQKIIKRVVGLQGDVVSTIGYRDPIVSVPQGHCWVEGDHTGHSLDSNTFGPVALGLMTAKAVAIVWPPERWRLLENELPRRRAPIQVAKTSHYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUK1</accession>
    <name>A0A6I8UUK1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360865.3">
        <property type="nucleotide sequence ID" value="XM_001360828.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUK1"/>
    <dbReference type="FunCoup" id="A0A6I8UUK1">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804284"/>
    <dbReference type="InParanoid" id="A0A6I8UUK1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505">
        <property type="entry name" value="MADF DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505:SF12">
        <property type="entry name" value="MADF DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="1">
        <location>
            <begin position="34"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360865.3"/>
        </source>
    </evidence>
    <sequence length="290" mass="32765" checksum="B8FE8E38B3B5BAF0" modified="2020-08-12" version="1">MSAPFDDVGCYSQAKRQGRVRRKPTIWSREKISKLIQLYRKSDCLWNHYSDLYKNKDCRTKATESICSSLGITKNDYGKKVHNLRNQFNSELKKLERRQEETGGDSSPMADKTTCRWEHFQSLMFLRSIIEPRPGYQQNGPAQCKKLSAKLKGVSNDGEEVETQSPPSVSKGQFESMDQLVTVVDESKAHQSICETIETCVPEYASTILSAPTTCTSSIPTRQVDASGTVSVPAHVSIARDQWDSFGELIANEFRNLNSDISRKRLKRKIMQVMLEVGEEDDRENAGPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVR8</accession>
    <name>A0A6I8UVR8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein Corin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Corin</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361394.4">
        <property type="nucleotide sequence ID" value="XM_001361357.5"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UVR8">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804909"/>
    <dbReference type="InParanoid" id="A0A6I8UVR8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07066">
        <property type="entry name" value="CRD_FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000019">
        <property type="entry name" value="Corin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000151">
        <property type="entry name" value="Corin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000226">
        <property type="entry name" value="Corin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="FZ" evidence="8">
        <location>
            <begin position="742"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="948"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="1072"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="244"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="352"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="435"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="565"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="372"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="577"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="824"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="883"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="895"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="913"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="920"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="932"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361394.4"/>
        </source>
    </evidence>
    <sequence length="1365" mass="148744" checksum="22FBF81340FFDC52" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWF4</accession>
    <name>A0A6I8UWF4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Luciferin 4-monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4805603</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361980.2">
        <property type="nucleotide sequence ID" value="XM_001361943.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWF4"/>
    <dbReference type="GeneID" id="4805603"/>
    <dbReference type="KEGG" id="dpo:4805603"/>
    <dbReference type="InParanoid" id="A0A6I8UWF4"/>
    <dbReference type="OMA" id="PMTHIFG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078267">
        <property type="expression patterns" value="Expressed in male reproductive system"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000025">
        <property type="entry name" value="luciferin 4-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="54"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="442"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361980.2"/>
        </source>
    </evidence>
    <sequence length="534" mass="58917" checksum="28D5400C9C2FBA37" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWP6</accession>
    <name>A0A6I8UWP6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-conjugating enzyme E2-18 kDa</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ubc10</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001361786.1">
        <property type="nucleotide sequence ID" value="XM_001361749.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWP6"/>
    <dbReference type="FunCoup" id="A0A6I8UWP6">
        <property type="interactions" value="2008"/>
    </dbReference>
    <dbReference type="GeneID" id="4805376"/>
    <dbReference type="KEGG" id="dpo:4805376"/>
    <dbReference type="InParanoid" id="A0A6I8UWP6"/>
    <dbReference type="OMA" id="ADLHTWH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079126">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23801">
        <property type="entry name" value="UBCc_UBE2L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000011">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361786.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="18010" checksum="A87B85BE5B047E92" modified="2020-08-12" version="1">MTAPRRLRKELCDLQDNTLKSFRDIKADDENLLRWTGLIVPDNPPYNKGAFRIEINFPAEYPFKPPKINFKTRIYHPNIDEKGQVCLPIISTENWKPATRTEQVVQALIALINDPEPEHPLRAELAEEFLKDRKKFVKNAEDYTKKHSEKRPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXU6</accession>
    <name>A0A6I8UXU6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proteasome activator complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6903270</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132969.2">
        <property type="nucleotide sequence ID" value="XM_002132933.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXU6"/>
    <dbReference type="KEGG" id="dpo:6903270"/>
    <dbReference type="InParanoid" id="A0A6I8UXU6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXU6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008537">
        <property type="term" value="C:proteasome activator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.180">
        <property type="entry name" value="Proteasome activator pa28, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.120">
        <property type="entry name" value="Proteasome activator pa28, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003186">
        <property type="entry name" value="PA28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036997">
        <property type="entry name" value="PA28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036996">
        <property type="entry name" value="PA28_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009077">
        <property type="entry name" value="Proteasome_activ_PA28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003185">
        <property type="entry name" value="Proteasome_activ_PA28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036252">
        <property type="entry name" value="Proteasome_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660:SF2">
        <property type="entry name" value="LD45860P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660">
        <property type="entry name" value="PROTEASOME REGULATOR PA28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02252">
        <property type="entry name" value="PA28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02251">
        <property type="entry name" value="PA28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47216">
        <property type="entry name" value="Proteasome activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome activator PA28 N-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator PA28 C-terminal" evidence="5">
        <location>
            <begin position="77"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002132969.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22797" checksum="00C5930DD2D0063E" modified="2020-08-12" version="1">MSSMSENASPYPKVFVTKGGAQLLAQGFPQKIVELNELLDSPMFKEVEREQGTPVEVEGQGDGGSHDQPEKKCSCPVPCTKAVWVIVQAAKPIVQAMLANATIIKKWVAYMVEKNVHSTSLAASILDDVQKAIGKIHSEANDSFKQISKFHAVRARRPSDVARYPGVEECRQDIFEILCEVRKVYSMLIEFADKDWNKLKDSDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXX5</accession>
    <name>A0A6I8UXX5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MICOS complex subunit MIC13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6902493</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MICOS complex subunit Mic13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133038.2">
        <property type="nucleotide sequence ID" value="XM_002133002.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXX5"/>
    <dbReference type="KEGG" id="dpo:6902493"/>
    <dbReference type="InParanoid" id="A0A6I8UXX5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXX5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026769">
        <property type="entry name" value="Mic13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31816">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31816:SF3">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15884">
        <property type="entry name" value="QIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026106300" description="MICOS complex subunit MIC13" evidence="10">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002133038.2"/>
        </source>
    </evidence>
    <sequence length="133" mass="15579" checksum="09629B8051245196" modified="2020-08-12" version="1">MVLVVLVKLSVVAGVAFATKQMGFWESPEHSVVLLENAKEHLRPYAADLKDRICCWKCQEKCKEEFEPKPWRDSWIESWNDTIKKVFHFLNQMPIYYYRFSEDVQQAVTDLAKEKDQKQTTPPKVDKTPAKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYK0</accession>
    <name>A0A6I8UYK0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133358.2">
        <property type="nucleotide sequence ID" value="XM_002133322.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYK0"/>
    <dbReference type="KEGG" id="dpo:6902424"/>
    <dbReference type="InParanoid" id="A0A6I8UYK0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UYK0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133358.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="10070" checksum="35DB8948D37FBA4F" modified="2020-08-12" version="1">MVMLNPIEIVCFYVVQPILDYLTYLMLEVHYVAYLAGLAVIGIVLGLAFSIISVIWYKSTLKDLPGQPTVAVEHVHSPEGPPKKKDQKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZW0</accession>
    <name>A0A6I8UZW0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein valois-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6901050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134529.2">
        <property type="nucleotide sequence ID" value="XM_002134493.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZW0"/>
    <dbReference type="KEGG" id="dpo:6901050"/>
    <dbReference type="InParanoid" id="A0A6I8UZW0"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007309">
        <property type="term" value="P:oocyte axis specification"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="Methylosome_Comp_WDR77"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="METHYLOSOME PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46853:SF1">
        <property type="entry name" value="METHYLOSOME PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002134529.2"/>
        </source>
    </evidence>
    <sequence length="379" mass="41872" checksum="9FBE4949961DD6A0" modified="2020-08-12" version="1">MFPQKLSEVYRTPGDHQPPADKNLAGNLEYPNLNAADVRAQPANISPQLHECWDSIAISEEKEYVLATNRRHGCDWRGMFFGYSESNVGHMTVENADFKLQSDHVVNIVRYADNNVLLVAPGDTQLQAWSTRSAVYNSKDPFFLFELDKAVAHNSPISHMSVFKADPQKCVSACASGHLKMWSIIEAGLKSFYHARVSHSDKFSGLATPAITASQFVTCDRSGCSRLWDARAGSPSSTTLFNDADRALSFTCAAWAAPSELQGENHVYLGDFDGNVHIADIRVPCKLQETIQCFENGIVSQLEINGAHLAVMSNMPASVKIARVNEDGKPEFIYTKENIHKRQTDAIWTDSRTLLTVGHGRKMAIHQLELANSAVDCGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V018</accession>
    <name>A0A6I8V018_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Homeobox protein B-H2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">B-H2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
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    <dbReference type="RefSeq" id="XP_002133933.3">
        <property type="nucleotide sequence ID" value="XM_002133897.3"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000399">
        <property type="entry name" value="Homeobox protein B-H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
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    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050848">
        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF5">
        <property type="entry name" value="VENT HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
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    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="375"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
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            <begin position="377"/>
            <end position="436"/>
        </location>
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            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="89"/>
            <end position="138"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <end position="182"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
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    <feature type="region of interest" description="Disordered" evidence="7">
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        <location>
            <begin position="549"/>
            <end position="660"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
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        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
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        <location>
            <begin position="202"/>
            <end position="219"/>
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        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
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        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="589"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="599"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="631"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="648"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002133933.3"/>
        </source>
    </evidence>
    <sequence length="660" mass="71848" checksum="7CE87883852B9072" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V072</accession>
    <name>A0A6I8V072_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Mucin-5AC</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC6901151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134627.3">
        <property type="nucleotide sequence ID" value="XM_002134591.3"/>
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        <property type="interactions" value="1"/>
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        <property type="component" value="Chromosome X"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
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        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
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        <location>
            <begin position="161"/>
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            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
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        <location>
            <begin position="174"/>
            <end position="212"/>
        </location>
    </feature>
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            <begin position="369"/>
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        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002134627.3"/>
        </source>
    </evidence>
    <sequence length="383" mass="41100" checksum="63617965F3F6FDB1" modified="2020-08-12" version="1">MYKTIRHGENSLQYTILPQNDDFRIEGKLSAASSSSSSSATATSKRSSAAGQRRRRSFFAYLGLIFVCTVIIGAVLIPFLVSAECLPNPTEWFLKTKAALTHSRPPTGAGVGAGSGKTLPGTPPTGSPMLQLGQSVGKTVKIVNRNGIEQFVLKVNKTVPASSTGTEERQQPLTPGSSTTTTTSTTTTTTTMAPQTSSSTSTTTAPTTTTREQPPPPQLLVPVSTSGYAAVVPPQGQQRHLPATMITTRIIQVPLLKSAAKKPIMPPVLAKTQGSAEQHQQAAKMEKGQNKSNSDWIKTHWAYIDPSTYFQWTGYKDEDSVLLPALLGFALIGMILIITVCLVARNKRTIVSSVRKRNRNDIEEQGAEDNATLLTTTNLSDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1D2</accession>
    <name>A0A6I8V1D2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar ribonucleoprotein protein MPP10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6899795</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the MPP10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135739.2">
        <property type="nucleotide sequence ID" value="XM_002135703.3"/>
    </dbReference>
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        <property type="interactions" value="1613"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899795"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012173">
        <property type="entry name" value="Mpp10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04006">
        <property type="entry name" value="Mpp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017300">
        <property type="entry name" value="snoRNP_Mpp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 7" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="243"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="475"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="558"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="621"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="654"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="567"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="621"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002135739.2"/>
        </source>
    </evidence>
    <sequence length="679" mass="77428" checksum="5E19D4FFE0DD5DD3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1E7</accession>
    <name>A0A6I8V1E7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="9">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4813130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_002134835.2">
        <property type="nucleotide sequence ID" value="XM_002134799.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1E7"/>
    <dbReference type="FunCoup" id="A0A6I8V1E7">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813130"/>
    <dbReference type="InParanoid" id="A0A6I8V1E7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00312">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000378">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026371818" description="Carboxylic ester hydrolase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="10">
        <location>
            <begin position="27"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002134835.2"/>
        </source>
    </evidence>
    <sequence length="545" mass="60949" checksum="9E4847E498E3BF58" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1Y3</accession>
    <name>A0A6I8V1Y3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Grl62c</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135214.3">
        <property type="nucleotide sequence ID" value="XM_002135178.3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900856"/>
    <dbReference type="InParanoid" id="A0A6I8V1Y3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002135214.3"/>
        </source>
    </evidence>
    <sequence length="417" mass="48491" checksum="24F54CB9CEB2BA08" modified="2020-08-12" version="1">MPSPQSQSQPQSKPKPLHKLLCVCHIVSYFNGQKWVCHLLCLFNLRYDEAQQSFAFGQQVLVGLAYTWCVWSGLGFVVLLPYAPCWTIDRYTVGAYGLPTAYCLARRRYLLRILNEMVRVHRRLQRAMGPLFCVDVKRAFCWALLVALELVATLYWEVASQEPXQIFFQLGFVLCWYLQILLHANSYVWLQAIYVVMNQAFEAPLSPSQRWIMMRRLLRIQPRLRRIQRNVADCFSVHVLSAMVLVSFKFFVSFATSPWDLEAPPEGAVGLRFEVLHLRYLANRCLLICSLFLVMNDFQNERNRFLTSLWAMPPETPDCRLHRAHQRTLAVVDLMILSGNRPSDLRPTSAILWEIRIESKVKCAATMKSFVKYLLMLLPVVSFVPLVAVSNGFDLRWERYVLQNDTLAKWQELTKYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V285</accession>
    <name>A0A6I8V285_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Probable cytochrome P450 313a4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6896948</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137133.2">
        <property type="nucleotide sequence ID" value="XM_002137097.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V285"/>
    <dbReference type="FunCoup" id="A0A6I8V285">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896948"/>
    <dbReference type="InParanoid" id="A0A6I8V285"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V285">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11057">
        <property type="entry name" value="CYP313-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF189">
        <property type="entry name" value="CYTOCHROME P450 4C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002137133.2"/>
        </source>
    </evidence>
    <sequence length="491" mass="56726" checksum="98A5651A7E34006B" modified="2020-08-12" version="1">MLTWTLWCGLLFVLWIYFLWSRRRFYLLTLKIPGPIGYPLLGMAHKLMRREDILNEFGIYMAKHGPTIFSWLGPIPFMIVSEPQVVQDILTSPHCVNKGIIYKAVDDGAGVGLFSLKDPRWSVHRKLLNPAFGHKVLLSFLPIFNKETAQLLSQLDALPGGGEKDLIPLLQSLTLGIATQTTMGCDVKSEENFMSNSLMERYQCCLETMTDMCFSPWLNSRWARQLSGKEANYYRAKTEIRAFIRKLIQKRLSEDTEDSIKSKDKNIFLNLATDLLRRGVFDWKNVEDESNIIVFGAFETTANTVYYVLMLLAMFPQYQERAFEEIKSLFPNSGDFEVSYEDTQQMVYLDLVLNESMRVIPPVPVVSRQTSQDLTLTNGIVVPKGVQIAINMFHMHRSKKIWGPEAESFNPDNFLPHNTRDKHPYAFIPFTKGIRNCIGWRYALISAKVTLAKLLRNYRFKTSFKFEDLYYVEDITMKLKAVPKLEIQKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2N0</accession>
    <name>A0A6I8V2N0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6897174</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137354.2">
        <property type="nucleotide sequence ID" value="XM_002137318.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2N0"/>
    <dbReference type="FunCoup" id="A0A6I8V2N0">
        <property type="interactions" value="956"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897174"/>
    <dbReference type="InParanoid" id="A0A6I8V2N0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000009">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="11">
        <location>
            <begin position="23"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="9">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137354.2"/>
        </source>
    </evidence>
    <sequence length="440" mass="46783" checksum="63BB37848DE47CB6" modified="2020-08-12" version="1">MRLLRSSKFFVPQARNSSTQAGRRRVVITGSGAVTPLGNNAQDSWRRVLAGESAISKLGPEFKGLPCQVAAQISRENLQLETHLSKTDIKLMSPATQLAVLAAEEALASGNLKPKELSEEQRERFGVCVGMGMFDLAEVYSTWNQLQRGYNRVSPFFVPRLLPSMACGHISMRHGLRGPNHSVSTACATGAHALGDAMRFIRSGDADVMLAGSGEACIDPLSIAGFCRLRALSTAFNDNPAVASRPFDKARDGFVMGEGAAVLLLEELEHARARGAPILAELLGYGLSGDAHHITSPSEDGSGATLAMQRALKDAGVSPEEISYVNAHATSTPTGDRIESHAVRRVFGEHTPQVQVSSTKGAHGHLLGSSGNLEALFVVHACAENILPPSINIEQLDVGVNVVTKACEWSQGQGRRVALKNSFGFGGTNASLCIASYSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2V8</accession>
    <name>A0A6I8V2V8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Solute carrier family 41 member 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6897296</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137455.1">
        <property type="nucleotide sequence ID" value="XM_002137419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2V8"/>
    <dbReference type="GeneID" id="6897296"/>
    <dbReference type="KEGG" id="dpo:6897296"/>
    <dbReference type="InParanoid" id="A0A6I8V2V8"/>
    <dbReference type="OMA" id="MCVAARF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248595">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000001">
        <property type="entry name" value="Solute carrier family 41 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF26">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 41 MEMBER 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="11">
        <location>
            <begin position="76"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="11">
        <location>
            <begin position="290"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137455.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="50733" checksum="6B672151E7871CB8" modified="2020-08-12" version="1">MAPKREKQTVEQPQEQPEVQEDSDDAIYREPWYSTLIQIVVPFFLAGMGTITAGIMLGNVQHWTVYKQMTELFVLVPVLCGLKGNLDMCVAARFCTHSNLGHLKNKGDTWKIVIGNMALVQVQAIVCALLLSTFTMAITSGVAHKLKFENFSTLTAAALITSSTACLILDSGLMVIILFSQRYSFNPDYMATPIVASIGDVVTISLLSFSSAYMYDLAKSYMWINVSIMACYLAVLLPLWLIVALRNSYTRPLLMMSWVPVLGALCISEFGGFVLCSSVEQFMGFAVFSPIINGIGGNLVCVQASNMGTILHRRSMLGQLPTDTRICEWPHRVYFYGTVYSRVSRILIIVSIPGNVILVYIADYLYKGHISVGPVFLGSFALTSLLQLLILLWCAHFLVHLLWKYRIDPDSSAIPYLTALGDGLGTGLLAVMFRLLRYFDKEYTANEPVFRIKLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2W1</accession>
    <name>A0A6I8V2W1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137549.2">
        <property type="nucleotide sequence ID" value="XM_002137513.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2W1"/>
    <dbReference type="KEGG" id="dpo:6897397"/>
    <dbReference type="InParanoid" id="A0A6I8V2W1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026149577" evidence="1">
        <location>
            <begin position="19"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137549.2"/>
        </source>
    </evidence>
    <sequence length="155" mass="17506" checksum="0565342B44A1A2E7" modified="2020-08-12" version="1">MRLIQALATLLLLASTLSQFADLPASIRVQLSPRLLYRRKCGIQTDDIDPVGSVRVLLEKMELDHLNFEVVQAIEIPTYGLLFEMKSYDQAIEIATTWKRILRHNAEYGVVDTCPRGDSACVGPDFELQQTERDFFVGPSRTSTNFVGPTFRRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2Z9</accession>
    <name>A0A6I8V2Z9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Antennal-specific protein OS-C isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Os-C</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137575.1">
        <property type="nucleotide sequence ID" value="XM_002137539.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2Z9"/>
    <dbReference type="GeneID" id="6897423"/>
    <dbReference type="KEGG" id="dpo:6897423"/>
    <dbReference type="InParanoid" id="A0A6I8V2Z9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247838">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V2Z9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026224162" evidence="2">
        <location>
            <begin position="28"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137575.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="15089" checksum="54D73B8F8738FB2D" modified="2020-08-12" version="1">MGFHGRRQMLLASFLLVLLQVGLQTEARPQEVITVAADENETEVVIKKEGGDGDGDGDDDDSSSEETVEDSEETARRRREVGSDDALLPRAGIPGQQIVPILLEAVLPSPDAVGDRIARSLQFLNNVKSSLDGFSEERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3P2</accession>
    <name>A0A6I8V3P2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial</fullName>
            <ecNumber evidence="5">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">E2K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC6897970</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5"/>
    <dbReference type="RefSeq" id="XP_002138069.2">
        <property type="nucleotide sequence ID" value="XM_002138033.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3P2"/>
    <dbReference type="FunCoup" id="A0A6I8V3P2">
        <property type="interactions" value="2018"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897970"/>
    <dbReference type="InParanoid" id="A0A6I8V3P2"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004309">
        <property type="entry name" value="PRK05704.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="17">
        <location>
            <begin position="80"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="167"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="186"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_002138069.2"/>
        </source>
    </evidence>
    <sequence length="479" mass="51316" checksum="D60488AD8AA881C5" modified="2020-08-12" version="1">MTGIISIITRRLPNSVQTIGLRAVRSSELKRCLRQYSRLVAVSVARQQQLLNQDANLRCQEVPRAITWQGFHTTSHLWQEQSVKVPPFADSIAEGDIKFTCKVGDSFAQDEAVMEIETDKTTVPVPAPFAGQITDILVKDGDTVKPGQELFKMKPGAAPAKAAGAAAPAPAPVAPKPAPAAAAPKPAAPAPAAARPPPPPPAAARPPPPPPAARPPPAAPRAAAPPPAALAQVKLPPADGTRQILGTRSEQRVKMNRMRQKIAARLKDAQNTTAMLTTFNEIDMSYAMNFRKQNLDAFVKKYGIKLGFMSIFSKASAYALQDQPVVNAVIDGTDMVYRDYVDISVAVATPRGLMVPVIRNVESMNYADIEITLAGLADKAKRDAITVEDMDGGTFTISNGGVFGSLMGTPIINPPQSAILGMHGIFERPIAVKGEVKIRPMMYVALTYDHRIIDGREAVLFLRKIKAAVENPAIIVAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V407</accession>
    <name>A0A6I8V407_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dynein light chain roadblock-type 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">robls54B</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138358.3">
        <property type="nucleotide sequence ID" value="XM_002138322.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V407"/>
    <dbReference type="FunCoup" id="A0A6I8V407">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898296"/>
    <dbReference type="InParanoid" id="A0A6I8V407"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="2">
        <location>
            <begin position="16"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138358.3"/>
        </source>
    </evidence>
    <sequence length="112" mass="12842" checksum="842A819D8FB45DA9" modified="2020-08-12" version="1">MLLDAPERPPRSLRYVEDAFDQISKRRNIRDIVILNDLGHPVKSTMGHDDSVQFAGLFQAIRGRLERGMKIIDPTDELLMLRVRTKTNEVLLTPDSKITVIVVQNAQDDFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V579</accession>
    <name>A0A6I8V579_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chitinase-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Cht12</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138949.1">
        <property type="nucleotide sequence ID" value="XM_002138913.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V579"/>
    <dbReference type="FunCoup" id="A0A6I8V579">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="GeneID" id="6898989"/>
    <dbReference type="KEGG" id="dpo:6898989"/>
    <dbReference type="InParanoid" id="A0A6I8V579"/>
    <dbReference type="OMA" id="LYHECRD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245508">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026340256" evidence="5">
        <location>
            <begin position="21"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="7">
        <location>
            <begin position="29"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="390"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002138949.1"/>
        </source>
    </evidence>
    <sequence length="469" mass="54476" checksum="8E527C91CDC96511" modified="2020-08-12" version="1">MWSLNCAFVLMIFMIYGCAAKDFKIFCQYDVGSYYYKNSSKFFSWNIDLRLCTHLVYGTGVGVDGETGDVTITDKYLLVENDMLNVAHRLKRDNVKKVMFTIGGWLEESSDFSKMAADARKRDRFFTSLVEFMYEWRFDGVQIDWRYPTQRGGKPEDRKNFILLLEELKIICQEHQFILMVAVLGRTDKSTLEWYDIPKLVQYVHYINLMHHDNRDPYQMHLSYNAPLSGVNSVIESVKLWKQLGKAPAKLILNIPLFVRSYIMENDKTTVGSPCKGPGLKTPLTREPGFMTYGEWCMYASSWTQKFDEQAQVPYAYKGNHWISYENARSIWAKMHLLKSNNLGGAMAWTIDSDDYHGKCGESHSLGRVILTALGDVSILAPEEPTTESMGLCPKNGFFRQLWDCQLYYECRNGERIDYECLEGYYFDESRIECMPKAMVKCDQNFVTWRPGQPLYNFENMPVNLKIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V602</accession>
    <name>A0A6I8V602_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC13036360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736776.2">
        <property type="nucleotide sequence ID" value="XM_003736728.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V602"/>
    <dbReference type="KEGG" id="dpo:13036360"/>
    <dbReference type="InParanoid" id="A0A6I8V602"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0263804">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_003736776.2"/>
        </source>
    </evidence>
    <sequence length="86" mass="9816" checksum="E2B2ADC9F6BFEE1C" modified="2020-08-12" version="1">MVYQWRNNLNSSMSMPLPLSESFGCTCTCICLSLIWLHLSLINELLDYLQLAVPLHLVRQRELEDAEQNIAGEYTLGAISIRMIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8S6</accession>
    <name>A0A6I8V8S6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein synr</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">synr</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036118.2">
        <property type="nucleotide sequence ID" value="XM_015180632.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V8S6"/>
    <dbReference type="KEGG" id="dpo:4817159"/>
    <dbReference type="InParanoid" id="A0A6I8V8S6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21974">
        <property type="entry name" value="RE15880P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21974:SF2">
        <property type="entry name" value="RE15880P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="166"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015036118.2"/>
        </source>
    </evidence>
    <sequence length="382" mass="43466" checksum="C481B60827F5A10F" modified="2020-08-12" version="1">MMGCGSSMETQSNPVVTEVSTEFRQQQNGAAVTNIIDEYVRLDEQISKMEGSCPGPRMATAEAWIEHLQGKHDAMLGLDGMEVAQRRQEEQQDTEMEALTLSSYPAQKRDGNQIVANTPTKDLAQLAYNLGVIGDARKASMHEDFFANLSESALYLLSIRYYGELLEHTKERLKTLAESYAELNELYVRQDGIIAYISGGTYMTRLEETIDEQLETARDTRDKLGSTLEQWRICGLLLRAAANSSTQALKQWRQLSRIIDPKQKLQWALDCRSLLHASTISLEAAQLALPHVELKYMSHRQVLAVKHCNTYLITDIANKARYEHTSRVFSTYEANMSKTCTWLYETFNNTLRIDFDKSERSVSRLSKTLRDHRAEVFSTARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9M7</accession>
    <name>A0A6I8V9M7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036670.2">
        <property type="nucleotide sequence ID" value="XM_015181184.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9M7"/>
    <dbReference type="KEGG" id="dpo:26532454"/>
    <dbReference type="InParanoid" id="A0A6I8V9M7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015036670.2"/>
        </source>
    </evidence>
    <sequence length="171" mass="19234" checksum="38433D9CEE43C9F9" modified="2020-08-12" version="1">MEADLAFLIDALSTPNTPLTRPYSAEQLQEIRDSFVSLQRLLRHNPLPVEEEPELSWRMPRSKAGGSLAGTREALEESPESSGRERKETVSEIIRGSDTFSIWSLDTDTDTYETRQSLSSDLIVTRRQTCTSTISYSTPVELVWSNSGYISQSTDSDTTITPDGHRYSLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAA4</accession>
    <name>A0A6I8VAA4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pupal cuticle protein 36a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TwdlT</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037041.1">
        <property type="nucleotide sequence ID" value="XM_015181555.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAA4"/>
    <dbReference type="FunCoup" id="A0A6I8VAA4">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="GeneID" id="4801127"/>
    <dbReference type="KEGG" id="dpo:4801127"/>
    <dbReference type="InParanoid" id="A0A6I8VAA4"/>
    <dbReference type="OMA" id="TRDCAAM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VAA4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF16">
        <property type="entry name" value="LP07342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026284533" evidence="2">
        <location>
            <begin position="16"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="3">
        <location>
            <begin position="130"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015037041.1"/>
        </source>
    </evidence>
    <sequence length="293" mass="27423" checksum="4989658AC7AB4505" modified="2020-08-12" version="1">MKAFILMSCLALAAARPEAGYNYNRPGGGGGSGGGFGGGSSGGFGGGSSGGFGGGSSGGFGGGSSGGFGGGSGGGGGGGFGGGFGGGSGGGGGGGGGFGGGFGGSSGGGFGGGGSIGGFGGGGGGGGGTTLVQKHIYVHVPPPEQDEVRQRPNLPVGQSQKHYKIIFIKAPSPPSYQAPVIPLQPQNEEKTLVYVLVKKPEDQQDIVIPTPAPTQPSKPEVYFIKYKTQKESGGISGGISGGISGGGSGGFTQSNSGSGYTTGGGDGGFTGGDSGSISAPSSNYGPPGKSGPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBC7</accession>
    <name>A0A6I8VBC7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037733.2">
        <property type="nucleotide sequence ID" value="XM_015182247.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBC7"/>
    <dbReference type="KEGG" id="dpo:4802315"/>
    <dbReference type="InParanoid" id="A0A6I8VBC7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015037733.2"/>
        </source>
    </evidence>
    <sequence length="90" mass="10715" checksum="6850AC64B8706534" modified="2020-08-12" version="1">MVEKERTGTTRLTKKTKKKKLRMKKMATRITTKQKKKTMTKMTRTKMRRQSMKNFVVTISTGYDSTPVRLAPDWACMRNYNHSLVHVYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VCF6</accession>
    <name>A0A6I8VCF6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Phosphatidylinositol transfer protein beta isoform isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5" type="primary">vib</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="3 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 5">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="3 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015038359.1">
        <property type="nucleotide sequence ID" value="XM_015182873.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038360.1">
        <property type="nucleotide sequence ID" value="XM_015182874.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038362.1">
        <property type="nucleotide sequence ID" value="XM_015182876.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VCF6"/>
    <dbReference type="GeneID" id="4803242"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078775">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VCF6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008525">
        <property type="term" value="F:phosphatidylcholine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08888">
        <property type="entry name" value="SRPBCC_PITPNA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF11">
        <property type="entry name" value="VIBRATOR, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="1">
        <location>
            <begin position="131"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015038359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015038360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015038362.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="34389" checksum="9679C64256C6F4D8" modified="2020-08-12" version="1">MQIKEFRVTLPLTVEEYQVAQLFSVAEASKENTGGGEGIEVLKNEPFDNFPLLGGKYTSGQYTYKIYHLQSKVPAYIRLLAPKGSLEIHEEAWNAYPYCRTIITNPKFMKDAFKIIIDTLHVGDAGDSENNPGYMDKNFLMHIESNHFDNDLGDLPNVHELTPDKLKAREVVHIDIANDPILPADFKPEEDPTTYQSKKTGRGPLLGADWKKRVDPVMTCYKLVTCEFKWFGLQTRVENFIQKSERRLFTNFHRQVFCSTDRWYGLTMEDIRAIEEKVKEELDKARQVGEVRGMRADAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VCS8</accession>
    <name>A0A6I8VCS8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phenoloxidase-activating factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prophenoloxidase-activating factor II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">LOC6897793</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137898.2">
        <property type="nucleotide sequence ID" value="XM_002137862.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038081.2">
        <property type="nucleotide sequence ID" value="XM_015182595.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VCS8"/>
    <dbReference type="KEGG" id="dpo:6897793"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000038">
        <property type="entry name" value="Serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041515">
        <property type="entry name" value="PPAF-2-like_Clip"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18322">
        <property type="entry name" value="CLIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634505" description="Phenoloxidase-activating factor 2" evidence="7">
        <location>
            <begin position="24"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="163"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002137898.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015038081.2"/>
        </source>
    </evidence>
    <sequence length="429" mass="46263" checksum="E769AF466F35DB8B" modified="2020-08-12" version="1">MNRYRNLCHLVILGAFLTLSAKGAPQQDLNAEKNIQEIFNTNIGTPTNNSPPLTQPSSGIGLVVTPEPIDPSSPLTNFESISGKTATCNCVPYYKCDPSTNSVTEDESFDGFGVIDIRFNDDDPVCPSSVDICCDGNRTRSETLNPTPLDKRPNQVRGCGIRNVGGLDFTLSGVTQNEAGFGEFPWTVALLHAGNFSYFCAGSLIHPQVVLTAVHCVETHSPGSFMVRAGEWDSQTTKERLPYQDRAVQRIITHPQFKSRNIANDFALVILAQSVTLDDHINVVCLPRQGETAAPSTICTSTGWGKDVFGSPGKYSVIMKSVPLPVVDFNTCQTRLRNTRLGPKFALDKSFMCAGGQRGVDTCQGDGGAPLACPIGLASENRYQQSGIVAWGIGCNDEVPAAYANVALARDWIDQQMLANGFGTAVYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDQ3</accession>
    <name>A0A6I8VDQ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">G protein pathway suppressor 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4803450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039082.1">
        <property type="nucleotide sequence ID" value="XM_015183596.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDQ3"/>
    <dbReference type="FunCoup" id="A0A6I8VDQ3">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="GeneID" id="4803450"/>
    <dbReference type="InParanoid" id="A0A6I8VDQ3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074297">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026094">
        <property type="entry name" value="GPS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22654">
        <property type="entry name" value="G PROTEIN PATHWAY SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22654:SF2">
        <property type="entry name" value="G PROTEIN PATHWAY SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15991">
        <property type="entry name" value="G_path_suppress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015039082.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="46893" checksum="2D03EBF506FF6D31" modified="2020-08-12" version="1">MPPASAVNSNNAAAQAAKAERAEKLRGALRKFIVADRQRRQGEYDAYCEQMRVRREEEERERENQQALEETRDEITKLDEQLADLHSQKHQLLVQLKKVLNEDETRKKQAKENELFAMQQAAATVATSVFLPTLRLQHHQQLAKSLLSFQPPPGNQSGKRGRSPSPPNHAYYKSTASFAQQKHDDYRRAADYARLSWNKTTAQYPSTGTLFYQTTAAPPTTQQQADARLPAIYNYALPLRQAYHVELPSATVSKASDTQSPKAQPMQVLHINLDQPPISQSDLVVQATSVANSLSIQTSKPQVTMEKLPERYHIEVKHDGAPQNHVPPPPHLLSEGVIFKPMMSELSMHPNVLQISTSQNSKTTGSITQGFPPGRGSSGHEGQLNRQQLSLLPGQTATPPVSSSGPVPPAAGQQLQYARRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGE4</accession>
    <name>A0A6I8VGE4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chromogranin-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC26532626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015044023.2">
        <property type="nucleotide sequence ID" value="XM_015188537.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VMN5"/>
    <dbReference type="KEGG" id="dpo:26533784"/>
    <dbReference type="InParanoid" id="A0A6I8VMN5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VMN5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF20">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE PIPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015044023.2"/>
        </source>
    </evidence>
    <sequence length="123" mass="14984" checksum="8CAB020759680AD7" modified="2020-08-12" version="1">MLNLDLDPFRPFNSPLAVQIAKRRVETEFAVVGTWEETNITLAVLEHYIPRYFARATMIYKIYQDSIINRNRNNRKPHVDADVRAMVRRNFTHEYDFYYFCKQRLYMQYIALKRTELERYSHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNC1</accession>
    <name>A0A6I8VNC1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Aminopeptidase</fullName>
            <ecNumber evidence="19">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25 26" type="primary">LOC4800958</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="25 26" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25 26">MV-25-SWS-2005</strain>
            <tissue evidence="25 26">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.</text>
            <dbReference type="EC" id="3.4.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17 19">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="19"/>
    <dbReference type="RefSeq" id="XP_001358140.3">
        <property type="nucleotide sequence ID" value="XM_001358103.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232522.1">
        <property type="nucleotide sequence ID" value="XM_033376631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNC1"/>
    <dbReference type="KEGG" id="dpo:4800958"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016285">
        <property type="term" value="F:alanyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000019">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000013">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000008">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF301">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19 25 26" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 19" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10 17 19" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634537" description="Aminopeptidase" evidence="20">
        <location>
            <begin position="20"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="919"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="23">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="21">
        <location>
            <begin position="276"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="22">
        <location>
            <begin position="582"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="18">
        <location>
            <position position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_001358140.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_033232522.1"/>
        </source>
    </evidence>
    <sequence length="939" mass="106215" checksum="704BCAF8A34B67FF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNX2</accession>
    <name>A0A6I8VNX2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein Malvolio isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Mvl</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232727.1">
        <property type="nucleotide sequence ID" value="XM_033376836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNX2"/>
    <dbReference type="FunCoup" id="A0A6I8VNX2">
        <property type="interactions" value="975"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803299"/>
    <dbReference type="InParanoid" id="A0A6I8VNX2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VNX2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF33">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033232727.1"/>
        </source>
    </evidence>
    <sequence length="614" mass="67868" checksum="508253B9BD21E888" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VRB0</accession>
    <name>A0A6I8VRB0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">TRPL translocation defect protein 14 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">Ttd14</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233599.1">
        <property type="nucleotide sequence ID" value="XM_033377708.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233600.1">
        <property type="nucleotide sequence ID" value="XM_033377709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VRB0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VRB0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.320.10:FF:000003">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038727">
        <property type="entry name" value="NadR/Ttd14_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053227">
        <property type="entry name" value="TRPL-trafficking_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34932">
        <property type="entry name" value="TRPL TRANSLOCATION DEFECT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34932:SF1">
        <property type="entry name" value="TRPL TRANSLOCATION DEFECT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13521">
        <property type="entry name" value="AAA_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NadR/Ttd14 AAA" evidence="2">
        <location>
            <begin position="202"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033233599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033233600.1"/>
        </source>
    </evidence>
    <sequence length="546" mass="61922" checksum="DB85F02503C45A6B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSI1</accession>
    <name>A0A6I8VSI1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234017.1">
        <property type="nucleotide sequence ID" value="XM_033378126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VSI1"/>
    <dbReference type="InParanoid" id="A0A6I8VSI1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026318181" evidence="1">
        <location>
            <begin position="25"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234017.1"/>
        </source>
    </evidence>
    <sequence length="211" mass="24094" checksum="1405C13518A1686C" modified="2020-08-12" version="1">MFRKYWLIWALALWVSCAPYAGQAKKQIVFNTMKCSLSGKVFANIECRLETRKALSLILTTDELKTADNVVGVFELRLSIYGQTKTITMKKIRLDLCQLRAQRDKKSLMRLFYKGLRTANHNLPDKCPFLRVSSSGPSWSIQDSSANIIFQNTTYFMRKVEIDASELPPYLPEYNITFGGRIYANNVLSSELFITGGFCEAEQDCTGQREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVJ3</accession>
    <name>A0A6I8VVJ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Prosalpha3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033234604.1">
        <property type="nucleotide sequence ID" value="XM_033378713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VVJ3"/>
    <dbReference type="FunCoup" id="A0A6I8VVJ3">
        <property type="interactions" value="2094"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805509"/>
    <dbReference type="InParanoid" id="A0A6I8VVJ3"/>
    <dbReference type="OMA" id="YVLNDNM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081689">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03752">
        <property type="entry name" value="proteasome_alpha_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000022">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033234604.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="29223" checksum="7A7F23C1F0C38A22" modified="2020-08-12" version="1">MARRYDSRTTIFSPEGRLYQVEYAMEAISHAGTCLGILAEDGILLAAECRSTNKLLDSAIPSEKIYRLNENMVCSVAGITSDANVLTAELRLIAQRYQFSYGEVIPCEQLVSHLCDIKQAYTQYGGKRPFGVSLLYMGWDNKYGYQLYQSDPSGNYGGWKATCIGNNFGAAISMLKQELADKPSIKLEEAKDLAVKVLSMTLDTTKLTPEKVEMATLQRVKNTTVYSVLDKPDVEKLIEKYNKLQAETEAAKKEKQAKQPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VY16</accession>
    <name>A0A6I8VY16_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Angiotensin-converting enzyme</fullName>
            <ecNumber evidence="13">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6904117</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 13">Belongs to the peptidase M2 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_033235957.1">
        <property type="nucleotide sequence ID" value="XM_033380066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VY16"/>
    <dbReference type="FunCoup" id="A0A6I8VY16">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6904117"/>
    <dbReference type="InParanoid" id="A0A6I8VY16"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008241">
        <property type="term" value="F:peptidyl-dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06461">
        <property type="entry name" value="M2_ACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001548">
        <property type="entry name" value="Peptidase_M2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514:SF44">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01401">
        <property type="entry name" value="Peptidase_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00791">
        <property type="entry name" value="PEPDIPTASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52011">
        <property type="entry name" value="PEPTIDASE_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3 10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6 13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 14" id="KW-0732">Signal</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026143008" description="Angiotensin-converting enzyme" evidence="14">
        <location>
            <begin position="18"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor 1" evidence="5">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor 2" evidence="7">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor 1" evidence="5">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor 2" evidence="7">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="506"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="6">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="133"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10 12">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10 12">
        <location>
            <begin position="522"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-10"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-11"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033235957.1"/>
        </source>
    </evidence>
    <sequence length="615" mass="70906" checksum="F88BEAE0C28FF8D8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0H0</accession>
    <name>A0A6I8W0H0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="7">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4816407</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Mutarotase that catalyzes the interconversion of beta-D-galactose and alpha-D-galactose during galactose metabolism. Beta-D-galactose is metabolized in the liver into glucose 1-phosphate, the primary metabolic fuel, by the action of four enzymes that constitute the Leloir pathway: GALM, GALK1 (galactokinase), GALT (galactose-1-phosphate uridylyltransferase) and GALE (UDP-galactose-4'-epimerase). Involved in the maintenance of the equilibrium between the beta- and alpha-anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1. Also active on D-glucose although shows a preference for galactose over glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>alpha-D-galactose = beta-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:28675"/>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="2">
            <dbReference type="Rhea" id="RHEA:28677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="7"/>
    <dbReference type="RefSeq" id="XP_033236845.1">
        <property type="nucleotide sequence ID" value="XM_033380954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0H0"/>
    <dbReference type="FunCoup" id="A0A6I8W0H0">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W0H0"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W0H0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000016">
        <property type="entry name" value="Glucose-6-phosphate 1-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033236845.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="36329" checksum="C1607A77D6D7A358" modified="2020-08-12" version="1">MATTAAAMAATSVVVLDRGNNTTCTINLHGATVVSWRVNNQEQLFVSKLASFDGKKAIRGGIPFVFRKFESISLDLAAQFGAWSFGPQHGFARITRWHLERAPDRLHNGDVEAIFSLMDNEFTRSIWNYQFRITYRLILREKELHFHIGVYNPSKELSFSFNMLLHTYLKVPDVRRCQITGLHGCHFIDKTRENALYQEGREVVTVNEWTDRIYQHTPQEHVITNVVSGRKMRLHKYNFPDTVIWNPWIDRSREMSDFGDDEFPNMLCVEAGNVTSPVILLPGTAFEASQILQQFKCPYDIINAPKKSKKKSKSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5P2</accession>
    <name>A0A6I8W5P2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Pickpocket protein 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ppk5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238602.1">
        <property type="nucleotide sequence ID" value="XM_033382711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5P2"/>
    <dbReference type="FunCoup" id="A0A6I8W5P2">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900891"/>
    <dbReference type="InParanoid" id="A0A6I8W5P2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF243">
        <property type="entry name" value="PICKPOCKET 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="471"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033238602.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="58367" checksum="11F6E04CD70F7A27" modified="2020-08-12" version="1">MSFLSRVKEKLWRNGQNRKKIFLRNTSLELLKYVIGDRHLWWIKLYFIIIFASLIYVAVNLAVRVFVKWEDTPVIIGISSTMTPISKIPFPAITVCNMNQAKKSKVEDLIPGSLGYSMLQKTCYKENTYGNFTKSNHRYKNETFPNFILNVSERCEDLIVSCMFHKKKLPCTDIFREIFVDEGLCCVFNLLHPYYLYKFRPTYIRDYTATNRFADIAVDWNPISGYPPKLPSSYYPRPGVGVGIAMGLQIILNGHVNDYFCSSTNGQGFKVLLYNPIDQPRMKESGLPAMIGHQTTFRIIARSFEALPSIRNIHRTKRQCIFSDEEELYFYRYYTRRNCESECDALFFLRLCDCIPYYLPLVFSNATVCDAAQFDCWNNAELQIFDKKRSECKDLCLTSCHDLTFFPDAFTTPFSQKDLKAQSDYLKNLSNEYIRKNLAVVNFYFTENYFRSNVRTSYTGPTEYMSLTGGIMSLMIGFSVIFIAEILYVVVLFFILLFFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8V5</accession>
    <name>A0A6I8W8V5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein split ends-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239712.1">
        <property type="nucleotide sequence ID" value="XM_033383821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W8V5"/>
    <dbReference type="KEGG" id="dpo:117184832"/>
    <dbReference type="InParanoid" id="A0A6I8W8V5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239712.1"/>
        </source>
    </evidence>
    <sequence length="190" mass="22047" checksum="14E7EA5D156857E0" modified="2020-08-12" version="1">MPLPSLGWRILRHDRAPAATHTERKRSVKQCGRCPVVDQEKGRGRERGHTVPEDRTTGDPKARYAISKYVSVPTQDAGLVLVLVLGQDADFRFMAGRQTKCLRFGQSLQGDADDDDDDDEFCGLLLKEQQEEGLRRKEQQEEGLRRKEQQEEGLRRKEQQEEGLRRKEQQEEGLRRKEQQWGEYLKEETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9B4</accession>
    <name>A0A6I8W9B4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cuticle collagen 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117184909</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239966.1">
        <property type="nucleotide sequence ID" value="XM_033384075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9B4"/>
    <dbReference type="KEGG" id="dpo:117184909"/>
    <dbReference type="InParanoid" id="A0A6I8W9B4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026007585" evidence="2">
        <location>
            <begin position="19"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239966.1"/>
        </source>
    </evidence>
    <sequence length="95" mass="8638" checksum="AB212D0EFD9B9215" modified="2020-08-12" version="1">MRILIVVALLALVAVATAQRSGGPPGGRPGGPPPRGPGGQPSGPGGPGGPGGPGGPGGPPSGPGGRPNGNSTEPPPSEAPSSEAPSSEAPSADSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAX7</accession>
    <name>A0A6I8WAX7_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein Spc105R isoform X1</fullName>
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    </protein>
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        <name evidence="3" type="primary">Spc105R</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240565.1">
        <property type="nucleotide sequence ID" value="XM_033384674.1"/>
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        <property type="interactions" value="38"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1538"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1664"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1762"/>
            <end position="1800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1847"/>
            <end position="1880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1768"/>
            <end position="1785"/>
        </location>
    </feature>
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        <location>
            <begin position="1847"/>
            <end position="1856"/>
        </location>
    </feature>
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        <location>
            <begin position="1871"/>
            <end position="1880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240565.1"/>
        </source>
    </evidence>
    <sequence length="2231" mass="253914" checksum="59B87165B5047E4E" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8WAY0</accession>
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            <fullName evidence="3 4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
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        <name evidence="3 4" type="primary">LOC6901302</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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                <consortium name="RefSeq"/>
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            <strain evidence="3 4">MV-25-SWS-2005</strain>
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            <begin position="35"/>
            <end position="132"/>
        </location>
    </feature>
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        <location>
            <begin position="39"/>
            <end position="102"/>
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    </feature>
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            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134003.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033240427.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="14935" checksum="5285C473C0CC8F3D" modified="2020-08-12" version="1">MNWKKIPVDDTVESFDSITTIQFFGENKKSTKILVKPKPRSEVSDKSDKLETAKKLPRSEVSDKSDKLETAKKLPRSEMTDNLETAKKLPRSEMTDNLETAKELPGSEMSDNLETAKESLHDAVDLQKPIQK</sequence>
</entry>
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    <accession>A0A6I8WBP2</accession>
    <name>A0A6I8WBP2_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
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        <name evidence="3" type="primary">LOC4815428</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Drosophila</taxon>
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        </lineage>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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            <begin position="77"/>
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            <begin position="103"/>
            <end position="123"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
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            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240821.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="26364" checksum="BA9706ED1B2257F8" modified="2020-08-12" version="1">MVNALLLVGSADPVRTSLPLLWGGAESLQPLLSGCLANGLEAVGWRGKGVDACAACTLIIHPLAALSTLLQPERTGSRLRFRRRQSQRHSHSRNERAQCDLRDLLGAVSDDGPDPGRELRSRIPRGVPGTLACGVSGVPVESRTCPICRSDDTVYLQLYLDFEEPPPSQNQSQGQSGSADSGIESVESGTEYSGIMREYENLLYETGVYREEIEYLNERIETLMLRNSQLKAKLEMSSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCC8</accession>
    <name>A0A6I8WCC8_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Dentin sialophosphoprotein</fullName>
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        <name evidence="10" type="primary">Muc18B</name>
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    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
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        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026090903" evidence="7">
        <location>
            <begin position="26"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="209"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="210"/>
        </location>
    </feature>
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        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
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            <begin position="142"/>
            <end position="153"/>
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    </feature>
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            <begin position="164"/>
            <end position="177"/>
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    </feature>
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        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033240214.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="27663" checksum="4428A0A97C57E97C" modified="2020-08-12" version="1">MFKLPMLLGTTVVVALLLVSGGAQAEASLCRQSGYFALVDNTPEFYACRASPAGGFSLQFMRCSAGSVFSSKAGHCTEVADLSLLARDDGNIENPTIPPSNVDDSSSKKPAEVTTPTPVPTAAPFTDIPENTTDAPISTDDVSTDDPSTDEPSSDTTEKPADVTTDSPISTEDPSSESTEKPAEGTTGSDSTDSSDSTDDGSTTAATPILDCPTIGFFPMDENCGEFIICFQNGNHLQAASFKCPEGMQFNPELSFCVADFDCTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDE9</accession>
    <name>A0A6I8WDE9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Patj homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117185198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241451.1">
        <property type="nucleotide sequence ID" value="XM_033385560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDE9"/>
    <dbReference type="KEGG" id="dpo:117185198"/>
    <dbReference type="InParanoid" id="A0A6I8WDE9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WDE9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033241451.1"/>
        </source>
    </evidence>
    <sequence length="105" mass="11327" checksum="28A0C752CC0A519F" modified="2020-08-12" version="1">MPVPVSYGWMGRRPRPGQVALLSMCRTLSELNAQLGQHPSMLPNDFDIDVAGNLVLSLNGGEVMYDFEDQQSISATRSPTSHSAPGSPNKSAGVEQRPQSQNSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEC2</accession>
    <name>A0A6I8WEC2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras modification protein ERF4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4801720</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with ERF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERF4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241738.1">
        <property type="nucleotide sequence ID" value="XM_033385847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEC2"/>
    <dbReference type="FunCoup" id="A0A6I8WEC2">
        <property type="interactions" value="1206"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801720"/>
    <dbReference type="InParanoid" id="A0A6I8WEC2"/>
    <dbReference type="OMA" id="FIYICTE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078888">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002178">
        <property type="term" value="C:palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7_ERF4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="7">
        <location>
            <begin position="28"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033241738.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="16623" checksum="D863F32CB970BCD9" modified="2020-08-12" version="1">MSQGGGTPAGSNAAALQAAGGGVVFSKVFIQRDYSVGTSVKFHTRLPTELEGIIERHVFESTINRLNEFYAEAEEGSCGTYCEGCIGCITAYLIYMCSETHYEKTLRKISKFVASQNERIYHQKGLQLIDPTFRGLRVIEITIFDRPGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>B3XWN8</accession>
    <accession>A0A6I8UNX6</accession>
    <name>B3XWN8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">H4</name>
        <name evidence="18" type="synonym">Dpse\GA31250</name>
        <name evidence="17" type="synonym">Dpse\GA31256</name>
        <name evidence="19" type="synonym">Dpse\GA31260</name>
        <name evidence="20" type="synonym">Dpse\GA31290</name>
        <name evidence="22" type="synonym">His4r</name>
        <name evidence="26" type="synonym">LOC117183563</name>
        <name evidence="27" type="synonym">LOC117183603</name>
        <name evidence="28" type="synonym">LOC117183610</name>
        <name evidence="29" type="synonym">LOC117185604</name>
        <name evidence="30" type="synonym">LOC117185605</name>
        <name evidence="31" type="synonym">LOC117185616</name>
        <name evidence="32" type="synonym">LOC117185617</name>
        <name evidence="33" type="synonym">LOC117185618</name>
        <name evidence="34" type="synonym">LOC117185619</name>
        <name evidence="36" type="synonym">LOC117185640</name>
        <name evidence="37" type="synonym">LOC117185641</name>
        <name evidence="38" type="synonym">LOC117185642</name>
        <name evidence="35" type="synonym">LOC26532670</name>
        <name evidence="23" type="synonym">LOC26532676</name>
        <name evidence="24" type="synonym">LOC26532680</name>
        <name evidence="25" type="synonym">LOC26532710</name>
        <name evidence="22" type="synonym">LOC4801366</name>
        <name evidence="18" type="ORF">Dpse_GA31250</name>
        <name evidence="17" type="ORF">Dpse_GA31256</name>
        <name evidence="19" type="ORF">Dpse_GA31260</name>
        <name evidence="20" type="ORF">Dpse_GA31290</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2006-02" db="EMBL/GenBank/DDBJ databases">
            <title>Population size effect for selection efficiency for the GC content of the histone genes in Drosophila lutescens, D.takahashii and D.pseudoobscura.</title>
            <authorList>
                <person name="Nakashima Y."/>
                <person name="Higashiyama A."/>
                <person name="Ushimaru A."/>
                <person name="Nagoda N."/>
                <person name="Matsuo Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
                <person name="Castrezana S."/>
                <person name="Celniker S.E."/>
                <person name="Chang J.L."/>
                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
                <person name="Clifton S.W."/>
                <person name="Comeron J.M."/>
                <person name="Costello J.C."/>
                <person name="Coyne J.A."/>
                <person name="Daub J."/>
                <person name="David R.G."/>
                <person name="Delcher A.L."/>
                <person name="Delehaunty K."/>
                <person name="Do C.B."/>
                <person name="Ebling H."/>
                <person name="Edwards K."/>
                <person name="Eickbush T."/>
                <person name="Evans J.D."/>
                <person name="Filipski A."/>
                <person name="Findeiss S."/>
                <person name="Freyhult E."/>
                <person name="Fulton L."/>
                <person name="Fulton R."/>
                <person name="Garcia A.C."/>
                <person name="Gardiner A."/>
                <person name="Garfield D.A."/>
                <person name="Garvin B.E."/>
                <person name="Gibson G."/>
                <person name="Gilbert D."/>
                <person name="Gnerre S."/>
                <person name="Godfrey J."/>
                <person name="Good R."/>
                <person name="Gotea V."/>
                <person name="Gravely B."/>
                <person name="Greenberg A.J."/>
                <person name="Griffiths-Jones S."/>
                <person name="Gross S."/>
                <person name="Guigo R."/>
                <person name="Gustafson E.A."/>
                <person name="Haerty W."/>
                <person name="Hahn M.W."/>
                <person name="Halligan D.L."/>
                <person name="Halpern A.L."/>
                <person name="Halter G.M."/>
                <person name="Han M.V."/>
                <person name="Heger A."/>
                <person name="Hillier L."/>
                <person name="Hinrichs A.S."/>
                <person name="Holmes I."/>
                <person name="Hoskins R.A."/>
                <person name="Hubisz M.J."/>
                <person name="Hultmark D."/>
                <person name="Huntley M.A."/>
                <person name="Jaffe D.B."/>
                <person name="Jagadeeshan S."/>
                <person name="Jeck W.R."/>
                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
                <person name="Li R."/>
                <person name="Lin C.F."/>
                <person name="Lin M.F."/>
                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
                <person name="Machado C.A."/>
                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
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                <person name="Wu C.I."/>
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                <person name="Arachi H."/>
                <person name="Azer M."/>
                <person name="Bachantsang P."/>
                <person name="Barry A."/>
                <person name="Bayul T."/>
                <person name="Berlin A."/>
                <person name="Bessette D."/>
                <person name="Bloom T."/>
                <person name="Blye J."/>
                <person name="Boguslavskiy L."/>
                <person name="Bonnet C."/>
                <person name="Boukhgalter B."/>
                <person name="Bourzgui I."/>
                <person name="Brown A."/>
                <person name="Cahill P."/>
                <person name="Channer S."/>
                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
                <person name="Costello M."/>
                <person name="D'Aco K."/>
                <person name="Daza R."/>
                <person name="De Haan G."/>
                <person name="DeGray S."/>
                <person name="DeMaso C."/>
                <person name="Dhargay N."/>
                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
                <person name="Dorjee K."/>
                <person name="Dupes A."/>
                <person name="Elong R."/>
                <person name="Falk J."/>
                <person name="Farina A."/>
                <person name="Faro S."/>
                <person name="Ferguson D."/>
                <person name="Fisher S."/>
                <person name="Foley C.D."/>
                <person name="Franke A."/>
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                <person name="Gadbois L."/>
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                <person name="Giannoukos G."/>
                <person name="Goode T."/>
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                <person name="Grewal S."/>
                <person name="Gyaltsen K."/>
                <person name="Hafez N."/>
                <person name="Hagos B."/>
                <person name="Hall J."/>
                <person name="Henson C."/>
                <person name="Hollinger A."/>
                <person name="Honan T."/>
                <person name="Huard M.D."/>
                <person name="Hughes L."/>
                <person name="Hurhula B."/>
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                <person name="Kamat A."/>
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                <person name="Kisner P."/>
                <person name="Lance K."/>
                <person name="Lara M."/>
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                <person name="Lipovsky A."/>
                <person name="Liu X."/>
                <person name="Liu J."/>
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                <person name="Lokyitsang T."/>
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                <person name="Lubonja R."/>
                <person name="Lui A."/>
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                <person name="Magnisalis V."/>
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                <person name="Mehta T."/>
                <person name="Meldrim J."/>
                <person name="Meneus L."/>
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                <person name="Mihalev A."/>
                <person name="Mihova T."/>
                <person name="Mittelman R."/>
                <person name="Mlenga V."/>
                <person name="Montmayeur A."/>
                <person name="Mulrain L."/>
                <person name="Navidi A."/>
                <person name="Naylor J."/>
                <person name="Negash T."/>
                <person name="Nguyen T."/>
                <person name="Nguyen N."/>
                <person name="Nicol R."/>
                <person name="Norbu C."/>
                <person name="Norbu N."/>
                <person name="Novod N."/>
                <person name="O'Neill B."/>
                <person name="Osman S."/>
                <person name="Markiewicz E."/>
                <person name="Oyono O.L."/>
                <person name="Patti C."/>
                <person name="Phunkhang P."/>
                <person name="Pierre F."/>
                <person name="Priest M."/>
                <person name="Raghuraman S."/>
                <person name="Rege F."/>
                <person name="Reyes R."/>
                <person name="Rise C."/>
                <person name="Rogov P."/>
                <person name="Ross K."/>
                <person name="Ryan E."/>
                <person name="Settipalli S."/>
                <person name="Shea T."/>
                <person name="Sherpa N."/>
                <person name="Shi L."/>
                <person name="Shih D."/>
                <person name="Sparrow T."/>
                <person name="Spaulding J."/>
                <person name="Stalker J."/>
                <person name="Stange-Thomann N."/>
                <person name="Stavropoulos S."/>
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                <person name="Tesfaye S."/>
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                <person name="Thoulutsang Y."/>
                <person name="Thoulutsang D."/>
                <person name="Topham K."/>
                <person name="Topping I."/>
                <person name="Tsamla T."/>
                <person name="Vassiliev H."/>
                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
                <person name="Weiand M."/>
                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
                <person name="Yu Q."/>
                <person name="Zembek L."/>
                <person name="Zhong D."/>
                <person name="Zimmer A."/>
                <person name="Zwirko Z."/>
                <person name="Jaffe D.B."/>
                <person name="Alvarez P."/>
                <person name="Brockman W."/>
                <person name="Butler J."/>
                <person name="Chin C."/>
                <person name="Gnerre S."/>
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                <person name="MacCallum I."/>
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            <dbReference type="PubMed" id="17994087"/>
            <dbReference type="DOI" id="10.1038/nature06341"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
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    </reference>
    <reference evidence="17" key="4">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
            <authorList>
                <person name="English A.C."/>
                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="5">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="21" key="6">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24 25">MV-25-SWS-2005</strain>
            <strain evidence="21 22">MV2-25</strain>
            <tissue evidence="22 23 24">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="22 27 28" key="7">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22 27 28">MV-25-SWS-2005</strain>
            <strain evidence="21">MV2-25</strain>
            <tissue evidence="22 27 28">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H4 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BAG68517.1"/>
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        <property type="protein sequence ID" value="KRT05593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="CH673954">
        <property type="protein sequence ID" value="KRT05597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="CH673954">
        <property type="protein sequence ID" value="KRT05600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="KRT05604.1"/>
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        <property type="nucleotide sequence ID" value="XM_015179643.2"/>
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        <property type="nucleotide sequence ID" value="XM_015179650.2"/>
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        <property type="nucleotide sequence ID" value="XM_015179654.2"/>
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        <property type="nucleotide sequence ID" value="XM_033377806.1"/>
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        <property type="nucleotide sequence ID" value="XM_033378099.1"/>
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        <property type="nucleotide sequence ID" value="XM_033378135.1"/>
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        <property type="nucleotide sequence ID" value="XM_033385741.1"/>
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    <dbReference type="RefSeq" id="XP_033241633.1">
        <property type="nucleotide sequence ID" value="XM_033385742.1"/>
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    <dbReference type="RefSeq" id="XP_033241646.1">
        <property type="nucleotide sequence ID" value="XM_033385755.1"/>
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    <dbReference type="RefSeq" id="XP_033241647.1">
        <property type="nucleotide sequence ID" value="XM_033385756.1"/>
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    <dbReference type="RefSeq" id="XP_033241648.1">
        <property type="nucleotide sequence ID" value="XM_033385757.1"/>
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    <dbReference type="RefSeq" id="XP_033241650.1">
        <property type="nucleotide sequence ID" value="XM_033385759.1"/>
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    <dbReference type="RefSeq" id="XP_033241726.1">
        <property type="nucleotide sequence ID" value="XM_033385835.1"/>
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    <dbReference type="RefSeq" id="XP_033241727.1">
        <property type="nucleotide sequence ID" value="XM_033385836.1"/>
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    <dbReference type="RefSeq" id="XP_033241728.1">
        <property type="nucleotide sequence ID" value="XM_033385837.1"/>
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        <property type="nucleotide sequence ID" value="XM_033385838.1"/>
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    <dbReference type="eggNOG" id="KOG3467">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="OMA" id="QKEHING"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
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        <property type="expression patterns" value="baseline"/>
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    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Histone-fold"/>
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        <property type="entry name" value="Histone_H4"/>
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        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CENP-T_C"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Histone-fold"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="15">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAG68517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KRT05593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KRT05597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KRT05600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KRT05604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001358473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_015035129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_015035136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_015035140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_033233697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_033233990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_033234026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_033241632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_033241633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_033241646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_033241647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_033241648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_033241650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_033241726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="RefSeq" id="XP_033241727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="RefSeq" id="XP_033241728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="RefSeq" id="XP_033241729.1"/>
        </source>
    </evidence>
    <sequence length="103" mass="11381" checksum="DF3EB26393BD208C" modified="2008-10-14" version="1">MTGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>B5DIX0</accession>
    <accession>A0A6I8UXU8</accession>
    <name>B5DIX0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Splicing factor 3B subunit 5</fullName>
            <shortName evidence="2">SF3b5</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Sf3b5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SF3B5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132861.1">
        <property type="nucleotide sequence ID" value="XM_002132825.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DIX0"/>
    <dbReference type="FunCoup" id="B5DIX0">
        <property type="interactions" value="621"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DIX0"/>
    <dbReference type="GeneID" id="6903160"/>
    <dbReference type="KEGG" id="dpo:6903160"/>
    <dbReference type="eggNOG" id="KOG3485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138804_3_1_1"/>
    <dbReference type="InParanoid" id="B5DIX0"/>
    <dbReference type="OMA" id="YDRFNIH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246958">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DIX0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009846">
        <property type="entry name" value="SF3b5/RDS3-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017089">
        <property type="entry name" value="Splicing_factor_3B_subunit_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978:SF0">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037010">
        <property type="entry name" value="Splicing_factor_3B_subunit_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002132861.1"/>
        </source>
    </evidence>
    <sequence length="85" mass="9957" checksum="91133BC196462BF2" modified="2008-10-14" version="1">MGERYNIHSQLEHLQSKYIGTGHADTTKFEWLTNQHRDSLASYMGHYDILNYFAIAENESKARVRFNLMERMLQPCGPPPEKLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>B5DK39</accession>
    <accession>A0A6I8UZ28</accession>
    <name>B5DK39_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Large ribosomal subunit protein uL18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">60S ribosomal protein L5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RpL5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133263.1">
        <property type="nucleotide sequence ID" value="XM_002133227.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DK39"/>
    <dbReference type="FunCoup" id="B5DK39">
        <property type="interactions" value="1695"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DK39"/>
    <dbReference type="GeneID" id="6902749"/>
    <dbReference type="KEGG" id="dpo:6902749"/>
    <dbReference type="eggNOG" id="KOG0875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056222_1_0_1"/>
    <dbReference type="InParanoid" id="B5DK39"/>
    <dbReference type="OMA" id="CQIASAH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0265237">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DK39">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000002">
        <property type="entry name" value="60S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025607">
        <property type="entry name" value="Ribosomal_uL18_C_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14204">
        <property type="entry name" value="Ribosomal_L18_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 13" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL18 C-terminal eukaryotes" evidence="11">
        <location>
            <begin position="236"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002133263.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="34021" checksum="CAA2E62A1C9518E7" modified="2008-10-14" version="1">MGFVKVVKNKQYFKRYQVKFRRRREGKTDYYARKRLTFQDKNKYNTPKYRLIVRLSNKDITVQIAYARIEGDRVVCAAYSHELPKYGIKVGLTNYAAAYCTGLLVARRVLNKLGLDSLYAGCTEVTGEEFNVEPVDDGPGAFRCFLDVGLARTTTGARVFGAMKGAVDGGLNIPHSVKRFPGYSAETKSFNADVHRAHIFGQHVADYMRTLEEEDEESFKRQFSRYIKLGIRADDLEDIYKKAHQAIRNDPTHKVAAKKTSGVTKKRWNAKKLTNEQRKTKIAAHKAAYVAKLQSETEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>B5DL50</accession>
    <accession>A0A6I8UZJ0</accession>
    <name>B5DL50_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133622.2">
        <property type="nucleotide sequence ID" value="XM_002133586.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DL50"/>
    <dbReference type="STRING" id="46245.B5DL50"/>
    <dbReference type="GeneID" id="6902091"/>
    <dbReference type="KEGG" id="dpo:6902091"/>
    <dbReference type="HOGENOM" id="CLU_922192_0_0_1"/>
    <dbReference type="InParanoid" id="B5DL50"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244115">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133622.2"/>
        </source>
    </evidence>
    <sequence length="339" mass="38601" checksum="06C5E62C8187B9FD" modified="2016-01-20" version="2">MYADSCVRNTYSFLKTITKISSIMYKINIFLIFWCAVMMQPLMGQGLRDKSSDISVPSADAEVSATTVASIMGSLMEKTPAGNYAAAETTPAMATGSPPAAVKSTPRPKAAKEYKKRKVARTHKPLFDESRFDSSMEQFDFHDAPKPRSPHRHKRQKRHRHAPTEKPFLEPYPLMQTEPPMPPRYPMRAFSSSDADTATSSRASPFSFYEKLRSPDLNFEVKVLEMLSPTMLIESVELKPYEHSQPTMVPQNMPGFDFLNATEYERYGDHSIYSPKKTHKRHSSPEKPPLFDILQPVLKPLLHSLRKMEMKTAMEMHNSGKEQPLSRRSFEDPTLESTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>B5DLP6</accession>
    <accession>A0A6I8VHA9</accession>
    <name>B5DLP6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6 7" type="primary">LOC6902294</name>
    </gene>
    <organism evidence="2 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4 5">MV-25-SWS-2005</strain>
            <tissue evidence="3 4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133821.1">
        <property type="nucleotide sequence ID" value="XM_002133785.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041363.1">
        <property type="nucleotide sequence ID" value="XM_015185877.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041364.1">
        <property type="nucleotide sequence ID" value="XM_015185878.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041365.1">
        <property type="nucleotide sequence ID" value="XM_015185879.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041366.1">
        <property type="nucleotide sequence ID" value="XM_015185880.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DLP6"/>
    <dbReference type="GeneID" id="6902294"/>
    <dbReference type="KEGG" id="dpo:6902294"/>
    <dbReference type="HOGENOM" id="CLU_1564534_0_0_1"/>
    <dbReference type="OMA" id="REEANQW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244496">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DLP6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015041363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015041364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015041365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015041366.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="21915" checksum="44FDA3BC985C4FAC" modified="2008-10-14" version="1">MAPKNRISRVPAAVAAGRPSDGKLSVGTQTGIQICVAPSRVPTRPKIITGKDQLSMIRRGVKRASQPKNFLIVPKERFITARALVAGTIPPPLANEKACQATPVGKELKIPKPKAVASFRPRQIPPIPPIPPIPPMEETLKESAHNKDVKRLKYLMEIVNADKPPNWSLASDMWREIDFLEDRIDAYQKQEPANRAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>B5DP00</accession>
    <accession>A0A6I8V130</accession>
    <name>B5DP00_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED22</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134625.1">
        <property type="nucleotide sequence ID" value="XM_002134589.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DP00"/>
    <dbReference type="SMR" id="B5DP00"/>
    <dbReference type="FunCoup" id="B5DP00">
        <property type="interactions" value="1695"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DP00"/>
    <dbReference type="GeneID" id="6901149"/>
    <dbReference type="KEGG" id="dpo:6901149"/>
    <dbReference type="eggNOG" id="KOG3304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117242_2_0_1"/>
    <dbReference type="InParanoid" id="B5DP00"/>
    <dbReference type="OMA" id="KQAECDQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243809">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DP00">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434:SF6">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002134625.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="16633" checksum="27B22AACA89ABEC2" modified="2008-10-14" version="1">MASGSRIPTLPQSKEALLKSYNARLKEDVRSMLENFEETLKLARRESHSQISRTTQCEQDALEMQIRASNMVRAGESLMKLVADLKQYLILNDFHSVNEAITNNSQLFRATQIECDKKLMKLRDEMAMDLYDLEEEYYTSIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPQ6</accession>
    <accession>A0A6I8V0L7</accession>
    <name>B5DPQ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134968.1">
        <property type="nucleotide sequence ID" value="XM_002134932.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPQ6"/>
    <dbReference type="GeneID" id="6900595"/>
    <dbReference type="KEGG" id="dpo:6900595"/>
    <dbReference type="HOGENOM" id="CLU_165695_0_0_1"/>
    <dbReference type="InParanoid" id="B5DPQ6"/>
    <dbReference type="OMA" id="NIYQQCT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245177">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DPQ6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002134968.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="14927" checksum="C5B6F42EC07D18F5" modified="2008-10-14" version="1">MPVEDIFPYLPQDVVDYLLDQKNQERLLNEGKKKLKEAMLKIEDQLKLLDGRQDDQICTISWGNIYQKSTVNNVRHALVGRLSKSMDAYLKSNRKLLRLHRQMYKDTAIVFHKWGNTAGVPRLGDEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>B5DQW1</accession>
    <accession>A0A6I8V0Z9</accession>
    <name>B5DQW1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6899911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135279.1">
        <property type="nucleotide sequence ID" value="XM_002135243.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DQW1"/>
    <dbReference type="GeneID" id="6899911"/>
    <dbReference type="KEGG" id="dpo:6899911"/>
    <dbReference type="eggNOG" id="ENOG502TBWI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115563_0_0_1"/>
    <dbReference type="InParanoid" id="B5DQW1"/>
    <dbReference type="OMA" id="WFTSMLN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002135279.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="17800" checksum="EE9C79660C74B350" modified="2008-10-14" version="1">MTFKTGGSRFDPKIFSNFTVQIVNGKMAIDMVIVSPLSQGLKTHLSFDFRLDKGRPYQSIYQHDINYCAMIRGAQESIYRRWFTSMLNFGNLATSCPIGRGYYYLHGWTLDARYIPSFFYVGDYRISVTLFYGRFKKNDENPLVEFYVEAVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>B5DRX3</accession>
    <accession>A0A6I8V1B1</accession>
    <name>B5DRX3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Ero1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Ero1L</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135623.1">
        <property type="nucleotide sequence ID" value="XM_002135587.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DRX3"/>
    <dbReference type="FunCoup" id="B5DRX3">
        <property type="interactions" value="1575"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DRX3"/>
    <dbReference type="GeneID" id="6900301"/>
    <dbReference type="KEGG" id="dpo:6900301"/>
    <dbReference type="eggNOG" id="KOG2608">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023061_2_2_1"/>
    <dbReference type="InParanoid" id="B5DRX3"/>
    <dbReference type="OMA" id="CYKDRLH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250015">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017205">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="105"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="149"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="407"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR017205-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_002135623.1"/>
        </source>
    </evidence>
    <sequence length="485" mass="55847" checksum="177DCD994BE69CB3" modified="2008-10-14" version="1">MSARGTRQVQRNLWASLLALLVILYWADPTGGYFAALDETETNKNCFCELEGSINDCSCDVDTVDHFNNMKIFPRLQSLLVKNFFRFYKVNLRQECPFWPDDSRCAIRFCQVENCEEQAIPQGIKDKGEHKEKAAAFKYSREAQSANGCSDAEDFDSALGFLDTSISDQAHREFELWAKHDEAEEDFCIVDDHEEGSQYVDLLLNPERYTGYRGESAHRIWKSIYLENCFGGNNETANKFSNYVPHLDLRNVCLEQRAFYRIISGLHSSINIHLCSKYLLSESKDFLDPQGIWGPNAQEFKRRFSPETTGGEGPHWLRNLYFIYLVELRALAKAAPYLRREDYYTGIAEEDDEVKLAINDLLSVIESFQSHFDENALFSSGIASIKFKSDYKEKFRNISRIMSCVGCDKCKLWGKLQTQGLGTALKILYSEKLNLATESGLWDRPHIEADPIFRLSRPEIVALFNAFGRLSNSIYEMENFRRVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>B5DV60</accession>
    <accession>A0A6I8V1S9</accession>
    <name>B5DV60_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BTB/POZ domain-containing protein 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6903697</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002136775.1">
        <property type="nucleotide sequence ID" value="XM_002136739.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DV60"/>
    <dbReference type="SMR" id="B5DV60"/>
    <dbReference type="STRING" id="46245.B5DV60"/>
    <dbReference type="GeneID" id="6903697"/>
    <dbReference type="KEGG" id="dpo:6903697"/>
    <dbReference type="eggNOG" id="KOG4350">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004253_0_0_1"/>
    <dbReference type="InParanoid" id="B5DV60"/>
    <dbReference type="OMA" id="INICGEY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243953">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048512">
        <property type="term" value="P:circadian behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000005">
        <property type="entry name" value="BTB/POZ domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052407">
        <property type="entry name" value="BTB_POZ_domain_cont_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002136775.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="42217" checksum="13C92DF7690730B4" modified="2008-10-14" version="1">MNHATSSSSVDIILGDLVLEDMDRFCMNELFSDVSFLVGDQSLPAHSVILAARSEYFCAMLYGGMSVLNERQIRLESVPLEAFKVILRYLYSGKLHISTLDASCEVLGLANMYCLLEVESALVKHLLENMTVSNVWMILDMGHTHNLSQLANGCLKYIDNNGDQMLEHDSFQMLSKQSLEEVLRRDSFKVREVKIFKAVCKWNRHNPNEDIKSVMSLVRLSLMSADELIQVVRPSKIAEPEKIADAIEKALLPKNQNLPYRCTNYPDKDVKFLNTSTEGIFIDLSWWWLINCMCVLPSKPFDTWNCAMEVSCDMTHWDRAGTGTFQLAIHCEYITFTTRPVRYIRMVDTESGRTDLINYVLIQVKLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>B5DV79</accession>
    <accession>A0A6I8V1Y5</accession>
    <name>B5DV79_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Mitochondrial import receptor subunit TOM20 homolog B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Dpse\GA29327</name>
        <name evidence="15" type="synonym">Tom20</name>
        <name evidence="13" type="ORF">Dpse_GA29327</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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            </authorList>
            <dbReference type="PubMed" id="17994087"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
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                <person name="English A.C."/>
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            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH675797">
        <property type="protein sequence ID" value="EDY71820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002136794.1">
        <property type="nucleotide sequence ID" value="XM_002136758.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DV79"/>
    <dbReference type="STRING" id="46245.B5DV79"/>
    <dbReference type="GeneID" id="6903678"/>
    <dbReference type="KEGG" id="dpo:6903678"/>
    <dbReference type="eggNOG" id="KOG4056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100000_1_1_1"/>
    <dbReference type="OMA" id="PPPIFQI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250685">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DV79">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.10:FF:000001">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM20 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022422">
        <property type="entry name" value="MAS20_rcpt_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01989">
        <property type="entry name" value="EUOM20RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EDY71820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002136794.1"/>
        </source>
    </evidence>
    <sequence length="166" mass="18330" checksum="ABA52CD74CF91B4B" modified="2008-10-14" version="1">MLEMNKTAIGIAAGVAGTLFIGYCIYFDKKRRNDPEYKKKVRERRRRSKKTGNTKAGIPNLNDHEAIEKYFLQEIQLGETLIARGDFETGVEHLANAIVVCGQPARLLQVLQSSLPAQVFAMLIVKMQEFGNRAAESNDIQVNVGGQQVDSKMLDSSSGAGIDDLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q292E8</accession>
    <name>CIAO1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cytosolic iron-sulfur protein assembly protein Ciao1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">Ciao1</name>
        <name type="ORF">GA11817</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL24914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q292E8"/>
    <dbReference type="SMR" id="Q292E8"/>
    <dbReference type="FunCoup" id="Q292E8">
        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q292E8"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0278560">
        <property type="protein sequence ID" value="FBpp0276998"/>
        <property type="gene ID" value="FBgn0071866"/>
    </dbReference>
    <dbReference type="GeneID" id="4803656"/>
    <dbReference type="KEGG" id="dpo:4803656"/>
    <dbReference type="CTD" id="9391"/>
    <dbReference type="eggNOG" id="KOG0645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_8_1"/>
    <dbReference type="InParanoid" id="Q292E8"/>
    <dbReference type="OMA" id="IREIRWS"/>
    <dbReference type="PhylomeDB" id="Q292E8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071866">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000136">
        <property type="entry name" value="Probable cytosolic iron-sulfur protein assembly protein CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0853">WD repeat</keyword>
    <feature type="chain" id="PRO_0000382489" description="Probable cytosolic iron-sulfur protein assembly protein Ciao1">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 1">
        <location>
            <begin position="12"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 2">
        <location>
            <begin position="57"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 3">
        <location>
            <begin position="101"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 4">
        <location>
            <begin position="146"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 5">
        <location>
            <begin position="192"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 6">
        <location>
            <begin position="250"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 7">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <sequence length="335" mass="37097" checksum="363ECC3B9A8A71F7" modified="2006-04-04" version="1">MGRLILEHTLQGHKGRIWGVAWHPKGNVFASCGEDKAIRVWSLSGNTWSTKTILSDGHKRTIREIRWSPCGQYLASASFDATTAIWSKSSGEFECNATLEGHENEVKSVSWSRSGGLLATCSRDKSVWIWEVAGDDEFECAAVLNAHTQDVKRVVWHPTKDILASASYDNTIKMFAESQLDSDWDCTATLSSHTSTVWSIDFDAEGDRLVSCSDDKTLKIWRAYHPGNDAGIATPDKQSVWKCVCTLSGQHSRAIYDVSWCKLTGLIATGCGDDGIRIFKETSDSKRDEPTFEQLTAEETAHEQDVNAVEWNPAVAGQLISCSDDGTIKIWKVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DI3</accession>
    <accession>A0A6I8W6R0</accession>
    <name>Q29DI3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LOW QUALITY PROTEIN: uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239055.1">
        <property type="nucleotide sequence ID" value="XM_033383164.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812424"/>
    <dbReference type="eggNOG" id="ENOG502S011">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041482_0_0_1"/>
    <dbReference type="InParanoid" id="Q29DI3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072797">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DI3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239055.1"/>
        </source>
    </evidence>
    <sequence length="552" mass="57285" checksum="6F058C2CF897C83C" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29F94</accession>
    <accession>A0A6I8UEK5</accession>
    <name>Q29F94_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein 27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4814182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354306.1">
        <property type="nucleotide sequence ID" value="XM_001354270.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29F94"/>
    <dbReference type="SMR" id="Q29F94"/>
    <dbReference type="STRING" id="46245.Q29F94"/>
    <dbReference type="GeneID" id="4814182"/>
    <dbReference type="KEGG" id="dpo:4814182"/>
    <dbReference type="eggNOG" id="KOG3591">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095001_1_0_1"/>
    <dbReference type="InParanoid" id="Q29F94"/>
    <dbReference type="OMA" id="IIVQGNH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078207">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29F94">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06526">
        <property type="entry name" value="metazoan_ACD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF13">
        <property type="entry name" value="HEAT SHOCK PROTEIN 22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354306.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="23808" checksum="F13B2F43F4DF6C9B" modified="2006-04-04" version="1">MTLVPTTYRDLSRELDRPRPLYQAPYDFQMYPYLWDDPRVWWPAHHSSRSDLLRPMDELVTRRVRNQLIQSNPYDWAYPMRWDNYYSGERVHVDEKGFRVDIDVRQFHPHEIVVKTNDDYVIVQGNHNRRSEGSNGLVERHFVRKYLLPRGYNANEVISDISTDGILTIKAPPPPPSKYYSPGERMIRVHETGKLALPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GH6</accession>
    <accession>A0A6I8UGF0</accession>
    <name>Q29GH6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein twisted gastrulation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tsg</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the twisted gastrulation protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355077.2">
        <property type="nucleotide sequence ID" value="XM_001355041.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GH6"/>
    <dbReference type="FunCoup" id="Q29GH6">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GH6"/>
    <dbReference type="GeneID" id="4814519"/>
    <dbReference type="KEGG" id="dpo:4814519"/>
    <dbReference type="eggNOG" id="ENOG502QRE9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082511_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GH6"/>
    <dbReference type="OMA" id="CCRECAF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073474">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030510">
        <property type="term" value="P:regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006761">
        <property type="entry name" value="Tsg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057726">
        <property type="entry name" value="Tsg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057635">
        <property type="entry name" value="Tsg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12312">
        <property type="entry name" value="TWISTED GASTRULATION PROTEIN HOMOLOG 1-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12312:SF16">
        <property type="entry name" value="TWISTED GASTRULATION PROTEIN HOMOLOG 1-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04668">
        <property type="entry name" value="Tsg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23782">
        <property type="entry name" value="Tsg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Tsg N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Tsg C-terminal" evidence="7">
        <location>
            <begin position="86"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355077.2"/>
        </source>
    </evidence>
    <sequence length="248" mass="27290" checksum="6BEF39B79E5A3111" modified="2008-10-14" version="2">MLLLLPLIYSGLLLSLSLVAANNDDGCNEVVCGSVVSKCLLTQSCQCKLNDCHCCKDCLNCLGELYTECCGCLDMCPKHTDALPSLTPRSEIGDVDGVPELFDTLTAEDDDQWSTMRFPMRAGIKHRLEGTNNGLGDRPKPGATINCTVIYVNSCIRSNKCKQQCESMGANSYRWFHDGCCECVGANCLNYGINESRCTACPEDQDLLTSDAIHAETEQDLERIFGVEDDAFADDMWDDYGDDDDQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GS0</accession>
    <accession>A0A6I8UG81</accession>
    <name>Q29GS0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 11</fullName>
            <shortName evidence="5">U3 snoRNA-associated protein 11</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4815075</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the UTP11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354983.1">
        <property type="nucleotide sequence ID" value="XM_001354947.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GS0"/>
    <dbReference type="SMR" id="Q29GS0"/>
    <dbReference type="FunCoup" id="Q29GS0">
        <property type="interactions" value="1063"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GS0"/>
    <dbReference type="GeneID" id="4815075"/>
    <dbReference type="KEGG" id="dpo:4815075"/>
    <dbReference type="eggNOG" id="KOG3237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061887_2_1_1"/>
    <dbReference type="InParanoid" id="Q29GS0"/>
    <dbReference type="OMA" id="DLKYVVM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074737">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015952">
        <property type="entry name" value="U3snoRNP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR015952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001354983.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="27202" checksum="57F0DEAD921B8348" modified="2006-04-04" version="1">MSSWKKANKSNQKVHRERHQPDARQHLGLLEKKKDYKKRALDAQRKEKTLKLLYKRAQNKNPDEFYHHMINSKLSDDVHHEKQAKDEHTPEQLSLMQTQDLKYVVMKRTIERRKIDRLKVELMDIDAGKANKHYTFDEQGRKQQATLGERLKQTEATKEPLAPNSTKQQRINELKKRVQRERELSIVQQKLLLKKQLKQPRLIKPQKLKPATADRAPVYEFRYERKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HY6</accession>
    <accession>A0A6I8UFA6</accession>
    <name>Q29HY6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cytokine receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dome</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354567.3">
        <property type="nucleotide sequence ID" value="XM_001354531.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="Q29HY6">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HY6"/>
    <dbReference type="GeneID" id="4814949"/>
    <dbReference type="KEGG" id="dpo:4814949"/>
    <dbReference type="eggNOG" id="ENOG502QTMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_260365_0_0_1"/>
    <dbReference type="InParanoid" id="Q29HY6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072885">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HY6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF3">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043332526" evidence="2">
        <location>
            <begin position="28"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="233"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="543"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="643"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="742"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1051"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1052"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1072"/>
            <end position="1102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354567.3"/>
        </source>
    </evidence>
    <sequence length="1318" mass="147661" checksum="5F0097C7BDEB915D" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IF7</accession>
    <accession>A0A6I8UHP2</accession>
    <name>Q29IF7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="7">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Pdha</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001355637.1">
        <property type="nucleotide sequence ID" value="XM_001355601.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IF7"/>
    <dbReference type="SMR" id="Q29IF7"/>
    <dbReference type="FunCoup" id="Q29IF7">
        <property type="interactions" value="695"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IF7"/>
    <dbReference type="GeneID" id="4815688"/>
    <dbReference type="KEGG" id="dpo:4815688"/>
    <dbReference type="eggNOG" id="KOG0225">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_5_2_1"/>
    <dbReference type="InParanoid" id="Q29IF7"/>
    <dbReference type="OMA" id="LGYEMPC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080024">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IF7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 7 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="8">
        <location>
            <begin position="71"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355637.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="43837" checksum="69688727683C6879" modified="2006-04-04" version="1">MLRTLSRVSELPLIVKQLQKNAAQAGVAKTNNYATEATVQVNRPFKLHRLDEGPATEVKLTKDEALKYYTQMQTIRRIETAAGNLYKEKIIRGFCHLYSGQEACAVGMKAAMRDVDNIISAYRVHGWTYLMGVSPIGVLAELTGAQSGCARGKGGSMHMYAPNFFGGNGIVGAQVPLGTGVGLACKYKGNGGMCLALYGDGAANQGQVFEAYNMAYLWKLPVIFVCENNNYGMGTSSERASCNTDYYTRGDALPGIWVDGMDVLAVRSATEFAINYVNTVGPLVMETNTYRYSGHSMSDPGTSYRTREEIQEVRQKRDPITSFKELCIELGLITTDEVKAIDLKVRKEIDEATAFAKSDAELAVSHLWTDVSSNNLETKLRGTNSFNLDHIQERKGVNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IF8</accession>
    <accession>A0A6I8UHN0</accession>
    <name>Q29IF8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein will die slowly</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">wds</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR5/wds family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355636.1">
        <property type="nucleotide sequence ID" value="XM_001355600.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IF8"/>
    <dbReference type="FunCoup" id="Q29IF8">
        <property type="interactions" value="2009"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IF8"/>
    <dbReference type="GeneID" id="4815574"/>
    <dbReference type="KEGG" id="dpo:4815574"/>
    <dbReference type="eggNOG" id="KOG0266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_1_1"/>
    <dbReference type="InParanoid" id="Q29IF8"/>
    <dbReference type="OMA" id="CKGHDTA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074538">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IF8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000029">
        <property type="entry name" value="WD repeat-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="5">
        <location>
            <begin position="62"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001355636.1"/>
        </source>
    </evidence>
    <sequence length="356" mass="38801" checksum="35E3A660ACDB04BD" modified="2006-04-04" version="1">MVPIGPVHPNHPGVVHPPQQPMPTAPTGPNSLQPNANVSGSSNSTSNKSSMSVKPNYTLKFTLAGHTKAVSAVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSRLLVSGSDDKTLKIWELSTGKSLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQSKEVVQKLQGHTDTVLCTACHPTENIIASAALENDKTIKLWKSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29J04</accession>
    <accession>A0A6I8UHF1</accession>
    <name>Q29J04_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Enoyl-CoA delta isomerase 2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4816051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355440.3">
        <property type="nucleotide sequence ID" value="XM_001355404.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29J04"/>
    <dbReference type="SMR" id="Q29J04"/>
    <dbReference type="STRING" id="46245.Q29J04"/>
    <dbReference type="GeneID" id="4816051"/>
    <dbReference type="KEGG" id="dpo:4816051"/>
    <dbReference type="eggNOG" id="KOG0016">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071912_0_0_1"/>
    <dbReference type="InParanoid" id="Q29J04"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073282">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29J04">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF1">
        <property type="entry name" value="ENOYL-COA DELTA ISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001355440.3"/>
        </source>
    </evidence>
    <sequence length="268" mass="30149" checksum="69D5F82868694CDB" modified="2016-01-20" version="3">MSMYECWAVSLQFGIAMSTAAEDNLFCRQFRELTIRRRGGLLVIQFQRWQRRTIYELMRALDAANADGDISIVVLSGEFAVGREGDCQPLALEQGPGREKRDEVEDRFIQQRAADFVMRSLAKKLLSCRKLLVAFVQGPCQGLGVTICCLCDLVYATEAAYFLLSLSHLHPCVEAGPSWSLPHIEVLLRLGERADARSALGSGFVASLAADAQHFWTRMDQHSRLPTASLLATKRHLLRPWREQLLAQLRKESTPLAAQRRRLPPAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q29J22</accession>
    <accession>A0A6I8UH28</accession>
    <name>Q29J22_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein a6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">a6</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355422.3">
        <property type="nucleotide sequence ID" value="XM_001355386.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29J22"/>
    <dbReference type="FunCoup" id="Q29J22">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29J22"/>
    <dbReference type="GeneID" id="4816037"/>
    <dbReference type="KEGG" id="dpo:4816037"/>
    <dbReference type="eggNOG" id="ENOG502TCW8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_657691_0_0_1"/>
    <dbReference type="InParanoid" id="Q29J22"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077686">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355422.3"/>
        </source>
    </evidence>
    <sequence length="469" mass="50566" checksum="A0BA4E6EE728724D" modified="2016-01-20" version="3">MTQRHSEPFYISPRLFDNRRLKRRRCKWMERLREHQQTCKAEMRAQQAFASKTERGRRRCPATLQRHHLLAAATLPADVTIDLLSDDDDDTNDVNAIVEPRPKPVPAGASSTCNSLFMHQPNLLLMPATTLSIHQHDGGGGGSRGYTQRRVPKHGMSQGNAVTTTCVLGRETSTSTHSQSLTESILITSDDENNDNAIAKYVRRQHSMRSPPPLAPLTLSETVEEVTVSLVPRNTTTANCQARLRHSHPPHSGNYKACNIPSAPPHTASTNGMGRAASSNSGHGNGNDGYLEVDVGSGITATLPDETTVHTVIANRIYELSLSKLREGLASSGVPEYTHDLLPEQLQKLSPALRAKVAPLVAPSPPTPISLKLSSDLSISLISDDDDCESSSINNTAGGLGSDLVYPVVAAEAHAAAKLLKQQQPQLSVVQHLQYAGGGFAPPVALALPVMAHTATTSTVPLPRRRKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KP3</accession>
    <accession>A0A6I8UIM9</accession>
    <name>Q29KP3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Voltage-dependent anion-selective channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Porin2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356073.2">
        <property type="nucleotide sequence ID" value="XM_001356037.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KP3"/>
    <dbReference type="SMR" id="Q29KP3"/>
    <dbReference type="FunCoup" id="Q29KP3">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KP3"/>
    <dbReference type="GeneID" id="4816581"/>
    <dbReference type="KEGG" id="dpo:4816581"/>
    <dbReference type="eggNOG" id="KOG3126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044399_2_0_1"/>
    <dbReference type="InParanoid" id="Q29KP3"/>
    <dbReference type="OMA" id="QLDCKTM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074369">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KP3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000012">
        <property type="entry name" value="Voltage-dependent anion-selective channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF70">
        <property type="entry name" value="GH26960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00185">
        <property type="entry name" value="EUKARYTPORIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0626">Porin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356073.2"/>
        </source>
    </evidence>
    <sequence length="297" mass="31949" checksum="EE5CB97712750E9E" modified="2008-10-14" version="2">MAPPSYPDLGKLARDLFRRGYHPGVWQLDCKSMSSPGIEFFTTGFASQDHSKVAGSLQSKYKIEDSGLTLTERWNTDSWFFGEIVQKDKLAQGLMLGLEGRFQPSSDSKDGKFKLGYVEDHFNFLTDMGISSSPIMNCSLVLGHKEFLGGLGCTFDLGDTSLSSWKVALGWSNDKATLHGELKDGDSWLASLFYKLNDQIDAAVELTKTGGGGGGPEPAEGEAEQAGGSDVTVGVGMIYRLEGDALIRAKINSKAELGLGYEQKVREGITASISAVLDGRNISDGNHKFGVGLALEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LT6</accession>
    <accession>A0A6I8UKC7</accession>
    <name>Q29LT6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Homocysteine S-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4817337</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356893.1">
        <property type="nucleotide sequence ID" value="XM_001356857.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LT6"/>
    <dbReference type="SMR" id="Q29LT6"/>
    <dbReference type="FunCoup" id="Q29LT6">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LT6"/>
    <dbReference type="GeneID" id="4817337"/>
    <dbReference type="KEGG" id="dpo:4817337"/>
    <dbReference type="eggNOG" id="KOG1579">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004914_3_2_1"/>
    <dbReference type="InParanoid" id="Q29LT6"/>
    <dbReference type="OMA" id="TECYEAQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070500">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008898">
        <property type="term" value="F:S-adenosylmethionine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033528">
        <property type="term" value="P:S-methylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.330:FF:000002">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051486">
        <property type="entry name" value="Hcy_S-methyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007020">
        <property type="entry name" value="PRK09485.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46015:SF1">
        <property type="entry name" value="HOMOCYSTEINE S-METHYLTRANSFERASE-LIKE ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46015">
        <property type="entry name" value="ZGC:172121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356893.1"/>
        </source>
    </evidence>
    <sequence length="331" mass="36719" checksum="72BFDE1E984E8CA6" modified="2006-04-04" version="1">MGLTRVLVKDGGFGTQMTVHVGNSVDGDPLWSARFNATNPAAIINTHLDFLQNGADIILTNTYQASVEGYMEYLELDEDQSIELIRNTVRLAHIAKEKYLTECYQAQLAVPEGYPLIIASIGPFGAHLHDGSEYTGSYADYVPAKTITDWHRIRIEACLEAGVDALAIETIPCQMEAEALVEMLCDDYPDVKFWVAFQCKDESTLAHGETFADAANAIWDMLAERNAQDKCLAVGVNCVHPKFVTSLFKSLNGDRSVEDQIPLVVYPNSGEVYDVVKGWEGREHCVPLANYVPEWSQLGAKIIGGCCRTYARDIRHIGEAIRNWNKLKKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NA0</accession>
    <accession>A0A6I8UJ88</accession>
    <name>Q29NA0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat extensin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4816939</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356379.3">
        <property type="nucleotide sequence ID" value="XM_001356343.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NA0"/>
    <dbReference type="FunCoup" id="Q29NA0">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NA0"/>
    <dbReference type="GeneID" id="4816939"/>
    <dbReference type="KEGG" id="dpo:4816939"/>
    <dbReference type="eggNOG" id="ENOG502T9F2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_612919_0_0_1"/>
    <dbReference type="InParanoid" id="Q29NA0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072104">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29NA0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031806">
        <property type="entry name" value="DUF4744"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15918">
        <property type="entry name" value="DUF4744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043534079" evidence="2">
        <location>
            <begin position="22"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4744" evidence="3">
        <location>
            <begin position="333"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356379.3"/>
        </source>
    </evidence>
    <sequence length="403" mass="43646" checksum="55A8258061FFD9ED" modified="2016-01-20" version="3">MYGRCSLLFVVLLCASGLAHAHFPEYCAECEQEVWEQVPCSHVPTTARPTTARPDSGSTARPPTTTTTVASTTQVSYPTTTGTSAEEITTQPTAYKKCYCECKLGCKEFCRKVATSGPKQQLTQISSTGDYAPGGQIEYTHVHQRKYFPKYGGGAGYAGLVGPVDGPKAYKPYPLVYSEHAAAASSPAANNINAPNYTLEELAQLLSTAYGGKKEYPAPPPPRPQPRAQLQPAPPSVYYSPVYKQQSAGPSIPLVSRVEPTPKRYPSIKVSAAAVASSSGVAVAKVKTPYEEKIAVATPPPSNIYDRTTSYPIVEEHTAYALPQTESYPIAPSQTESYPSITEAYSRQTESYPSQTESYPSRSEEHPLQEPEPNKPSSSFDLAIDNYLKDFGYGNQGRGYADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NK7</accession>
    <accession>A0A6I8VY29</accession>
    <name>Q29NK7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA (cytosine(38)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.204</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">DNA (cytosine-5)-methyltransferase-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Mt2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.204" evidence="4"/>
    <dbReference type="RefSeq" id="XP_033235972.1">
        <property type="nucleotide sequence ID" value="XM_033380081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NK7"/>
    <dbReference type="FunCoup" id="Q29NK7">
        <property type="interactions" value="984"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NK7"/>
    <dbReference type="KEGG" id="dpo:4817726"/>
    <dbReference type="eggNOG" id="KOG0919">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049101_0_0_1"/>
    <dbReference type="InParanoid" id="Q29NK7"/>
    <dbReference type="OMA" id="HYAFKYA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070556">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29NK7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050750">
        <property type="entry name" value="C5-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031303">
        <property type="entry name" value="C5_meth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46098">
        <property type="entry name" value="TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46098:SF1">
        <property type="entry name" value="TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00095">
        <property type="entry name" value="C5_MTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033235972.1"/>
        </source>
    </evidence>
    <sequence length="347" mass="39747" checksum="83303CA4BFA5F5B9" modified="2008-10-14" version="2">MGFRVLELFSGIGGMHYAFKYAQLDGNIVAAMDVNTVANAVYNFALNCHVKTRNIQSLSEKEVSKLGATMLLMSPPCQPHTRQGLQRDTEDKRSDALTHLCTLIPQCETLQYILMENVKGFECSQARNQFVEALEKAGFYWREFILTPTQFNVPNTRYRYYCIARKTKDFAFAGGKIWEEMPESPSTEQSVSQISAILEDNVSCEYLVPDDVLTKRVLVMDIIHPTQNRSMCFTKGYTHYTEGTGSAFTPLSKEESHRIFELVKEIDNNNQDTSSSSEDVRQRRLDLLRQIKLRYFTPREVARLMSFPEEFAFPPETTNRQKYRLLGNSINVKVVGELIKLLIATKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LYK7</accession>
    <accession>A0A6I8VKW2</accession>
    <name>Q2LYK7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">Mkrn1</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001354116.1">
        <property type="nucleotide sequence ID" value="XM_001354080.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043490.1">
        <property type="nucleotide sequence ID" value="XM_015188004.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LYK7"/>
    <dbReference type="STRING" id="46245.Q2LYK7"/>
    <dbReference type="GeneID" id="4813957"/>
    <dbReference type="KEGG" id="dpo:4813957"/>
    <dbReference type="eggNOG" id="KOG1039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040815_1_0_1"/>
    <dbReference type="OMA" id="CRYFANN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080159">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000117">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase makorin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF10">
        <property type="entry name" value="IP09428P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="197"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="9">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="9">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001354116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015043490.1"/>
        </source>
    </evidence>
    <sequence length="367" mass="41934" checksum="BFEE8DBE9714A4DA" modified="2006-02-21" version="1">MSAVANSDPAIPNMALGRSQTICRFYLRGICRFGELCRFSHDLSRGRPECAEQTHITTDNAESIKPSTSQRNWANAPIFVPSQKRVLEQNEDEEAASGSVSLQNGISWAEVVGGPTVDVSCCEPASLDEVCPYECPCPYGEYCPYRIHMELCEMCDQYCLHPTDQAQRKNHNRECLQQHEQAMELSFAIARSKDKACGICFDTIMEKAGREKRFGILPNCNHIFCLECIRKWRQAKQFEHKITRACPECRVCSDFVCPSAFWVETKEEKDKLLNDYRAALGAKDCKYFQKGEGKCPFGNKCFYKHALPNGDIVDVGLPKRTRKLQGQHEIIDLLDIYLWDYVDRRDYHWLEIFSSDISESSDVSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M036</accession>
    <accession>A0A6I8UD53</accession>
    <name>Q2M036_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353587.2">
        <property type="nucleotide sequence ID" value="XM_001353551.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M036"/>
    <dbReference type="FunCoup" id="Q2M036">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M036"/>
    <dbReference type="GeneID" id="4813563"/>
    <dbReference type="KEGG" id="dpo:4813563"/>
    <dbReference type="eggNOG" id="ENOG502SC6M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181007_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M036"/>
    <dbReference type="OMA" id="KHHSTQA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070941">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M036">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019034">
        <property type="entry name" value="UPF0390"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09495">
        <property type="entry name" value="DUF2462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353587.2"/>
        </source>
    </evidence>
    <sequence length="105" mass="11221" checksum="FB9180C4C5886119" modified="2008-10-14" version="2">MPQGKFKKAKMPAAMAKKKQKQAAFTRRSNAPIQAKKGKFNETQKIKAVISKSVNKSVESELRSRAHEGYIQLSKAQEAVAKHHASQAAAAAATSTSASTSKPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0J1</accession>
    <accession>A0A6I8UCP0</accession>
    <name>Q2M0J1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E3 ubiquitin-protein ligase NRDP1</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RING finger protein 41</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase NRDP1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">elgi</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001353432.1">
        <property type="nucleotide sequence ID" value="XM_001353396.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0J1"/>
    <dbReference type="SMR" id="Q2M0J1"/>
    <dbReference type="FunCoup" id="Q2M0J1">
        <property type="interactions" value="1146"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0J1"/>
    <dbReference type="GeneID" id="4813211"/>
    <dbReference type="KEGG" id="dpo:4813211"/>
    <dbReference type="eggNOG" id="KOG0297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076732_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M0J1"/>
    <dbReference type="OMA" id="QYENYVC"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074313">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0J1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16634">
        <property type="entry name" value="mRING-HC-C3HC3D_Nrdp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000268">
        <property type="entry name" value="E3 ubiquitin-protein ligase NRDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000302">
        <property type="entry name" value="E3 ubiquitin-protein ligase NRDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015036">
        <property type="entry name" value="NRDP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037255">
        <property type="entry name" value="NRDP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF157">
        <property type="entry name" value="RECEPTOR-ASSOCIATED FACTOR, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08941">
        <property type="entry name" value="USP8_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160088">
        <property type="entry name" value="NRDP1 C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001353432.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="36102" checksum="2A9DF9EFCD86F985" modified="2006-02-21" version="1">MGYDVNRFQGEVDEELTCPICSGVLEDPLQAVMCEHAFCRGCINEWLTRQPTCPVDRNALTTANLRAVPRILRNLLSRLSITCDNAPYGCTAVLKLDVYNSHLEDCIHNPKRPFPCEKGCGFDIPKDELKDHNCVRELRTLIVKQTEKMGELKSELTDQQLTINELKRELQLFKDFMRAMRVSNPAMRAIADQMERDEVIRWSSALPRARVTRWGGMISTPDDALQLMIKRALSESGCPPHILDSLMEFCHERRWPRGLSSLETRQNNRRIYDNYICRRIPGKQAVLVLSCDNLHMTEDVMIDPGLVMIFAHGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M1B6</accession>
    <accession>A0A6I8UC79</accession>
    <name>Q2M1B6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4812831</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mediates sugar transport across membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353157.1">
        <property type="nucleotide sequence ID" value="XM_001353121.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M1B6"/>
    <dbReference type="FunCoup" id="Q2M1B6">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="GeneID" id="4812831"/>
    <dbReference type="KEGG" id="dpo:4812831"/>
    <dbReference type="eggNOG" id="KOG1623">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048643_3_3_1"/>
    <dbReference type="InParanoid" id="Q2M1B6"/>
    <dbReference type="OMA" id="CFVCNDI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080222">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M1B6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000004">
        <property type="entry name" value="Sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF5">
        <property type="entry name" value="SUGAR TRANSPORTER SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12 14" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001353157.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="24990" checksum="452EAB1638711963" modified="2006-02-21" version="1">MEALGDLLAPHSELIAKVAGTITTLQFLSGIALLNDIRKKGSSDVYPVGPFLGGVVLTVLSLKLANIMNDAAMINTNLIGLVINFVFLGGFYYYASSGSRGNIWKQIGYASIFLLACTAYANFEDPKKIEFRLGMLITGILVWLVGSPLLHLPKIIAKKSTEGMPFPIILSGNLVATSWMLYAISIKNTVMVLQNLLLLILGGIQLSMFAIYPNKPAAKKPAAKKDSKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P6Q9</accession>
    <accession>A0A6I8VMI8</accession>
    <name>A0A0R3P6Q9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">JNK-interacting protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Aplip1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043937.1">
        <property type="nucleotide sequence ID" value="XM_015188451.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P6Q9"/>
    <dbReference type="FunCoup" id="A0A0R3P6Q9">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P6Q9"/>
    <dbReference type="GeneID" id="4812463"/>
    <dbReference type="InParanoid" id="A0A0R3P6Q9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071376">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P6Q9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01212">
        <property type="entry name" value="PTB_JIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11801">
        <property type="entry name" value="SH3_JIP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000032">
        <property type="entry name" value="Putative C-Jun-amino-terminal kinase-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000306">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047178">
        <property type="entry name" value="JIP1_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437:SF4">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="221"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015043937.1"/>
        </source>
    </evidence>
    <sequence length="496" mass="54411" checksum="A539939E0D930496" modified="2016-01-20" version="1">MADSEFEEFHRPIFEPHTIATFGAGAAANKKNNSHAFYSLIPNDDMEDSTSSKSDGDGSDQEDGIGGMGMAEREREPKLRQVEDDELGDGLKVTLSSDGSLDTNDSFNSHRHHPLNHQDAIGGIMGMAPNALSSAPVTIGASTDLLTPNAAATRRRRKLPEIPKNQKSSILHLLGGTNYGSLADEFRTGGGGIIGGPLAAPRTGQQRSFLSLKCGYLMDEDSSPDSERLQSLGDVDSGHSTAHSPNDFKSMSPQITSPVSQSPFPPPFGGVPFGQLEMLEATHRGLHKFVPRHHDEIELEIGDAIYVQKEAEDLWCEGVNLRTGRQGIFPSAYAVDLDYNEFDPTVQLVKKERYLLGYLGSVETLAHKGTGVVCQAVRKIVGEYGASPTGQTCILEVSDQGLRMVDRSGPNNKKEKKPCIDYFYSLKNVSFCAFHPRDHRFIGFITKHPTVQRFACHVFKGSESTRPVAEAVGRAFQRFYQKFIETAYPIEDIYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAG1</accession>
    <name>A0A6I8UAG1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-disulfide reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">Trxr2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Thioredoxin system is a major player in glutathione metabolism, due to the demonstrated absence of a glutathione reductase. Functionally interacts with the Sod/Cat reactive oxidation species (ROS) defense system and thereby has a role in preadult development and life span. Lack of a glutathione reductase suggests antioxidant defense in Drosophila, and probably in related insects, differs fundamentally from that in other organisms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="15">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001352389.3">
        <property type="nucleotide sequence ID" value="XM_001352353.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAG1"/>
    <dbReference type="FunCoup" id="A0A6I8UAG1">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812191"/>
    <dbReference type="InParanoid" id="A0A6I8UAG1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000190">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000004">
        <property type="entry name" value="Thioredoxin reductase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006338">
        <property type="entry name" value="Thioredoxin/glutathione_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01438">
        <property type="entry name" value="TGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF7">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 15 17" id="KW-0274">FAD</keyword>
    <keyword evidence="4 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11 17" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="19">
        <location>
            <begin position="30"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="18">
        <location>
            <begin position="383"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="215"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="16">
        <location>
            <begin position="76"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001352389.3"/>
        </source>
    </evidence>
    <sequence length="512" mass="55879" checksum="CDBB6BFB2170E17B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAJ1</accession>
    <name>A0A6I8UAJ1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MIT domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4812510</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352426.3">
        <property type="nucleotide sequence ID" value="XM_001352390.4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="dpo:4812510"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MITD, C-terminal phospholipase D-like domain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR052817">
        <property type="entry name" value="MIT_domain_contain_protein1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR045331">
        <property type="entry name" value="MITD1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21222">
        <property type="entry name" value="MIT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21222:SF1">
        <property type="entry name" value="MIT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="4">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352426.3"/>
        </source>
    </evidence>
    <sequence length="241" mass="28239" checksum="42A6B7B5196C25F3" modified="2020-08-12" version="1">MSGALKAKEQLIRAVECDQIGRILEAQTLYQDGITQLMSFVETEPDENKRKAFVLRIKEYIDRADAIKARVNGKLMLGQVVDHISIEENDTGYDYDQIFGKYMDDKTIEILLEEPYMTQNYQYQNLIRFLELAITNCKNLKYFRLITKEYSDPKNPDQQKTNLGQIRADLERRYIVVSIKYEDTLHDRRIYLSNGYIIKIGRGLHFYKAANPMYSIGLVNYKYRKCLQTDVDVWRNNSAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBQ4</accession>
    <name>A0A6I8UBQ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Protein artichoke</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">atk</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352983.3">
        <property type="nucleotide sequence ID" value="XM_001352947.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044064.2">
        <property type="nucleotide sequence ID" value="XM_015188578.2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811878"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001186">
        <property type="entry name" value="GL12775"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634419" evidence="5">
        <location>
            <begin position="20"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="6">
        <location>
            <begin position="919"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1296"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1385"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1296"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1328"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1385"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1412"/>
            <end position="1421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352983.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015044064.2"/>
        </source>
    </evidence>
    <sequence length="1549" mass="173615" checksum="00F453BAF40BCB96" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCG9</accession>
    <name>A0A6I8UCG9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4813312</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353288.4">
        <property type="nucleotide sequence ID" value="XM_001353252.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCG9"/>
    <dbReference type="FunCoup" id="A0A6I8UCG9">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813312"/>
    <dbReference type="InParanoid" id="A0A6I8UCG9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCG9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF84">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="676"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="749"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="795"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="839"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="966"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1016"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1117"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="11">
        <location>
            <begin position="347"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="10">
        <location>
            <begin position="583"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001353288.4"/>
        </source>
    </evidence>
    <sequence length="1236" mass="141758" checksum="96FA5AC53BBFC1CA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCN9</accession>
    <name>A0A6I8UCN9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">carbonic anhydrase</fullName>
            <ecNumber evidence="2">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CAH2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001353354.2">
        <property type="nucleotide sequence ID" value="XM_001353318.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCN9"/>
    <dbReference type="FunCoup" id="A0A6I8UCN9">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813361"/>
    <dbReference type="InParanoid" id="A0A6I8UCN9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCN9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00326">
        <property type="entry name" value="alpha_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000003">
        <property type="entry name" value="Carbonic anhydrase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF124">
        <property type="entry name" value="CARBONIC ANHYDRASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026163707" description="carbonic anhydrase" evidence="6">
        <location>
            <begin position="25"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="7">
        <location>
            <begin position="25"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353354.2"/>
        </source>
    </evidence>
    <sequence length="335" mass="37911" checksum="B88F546AA6693AB8" modified="2020-08-12" version="1">MWRCNVSATVAFALILAYASSGLAQDFGYGGQHGPEHWSEDYNRCSGKQQSPINIDLVNVEDKTYPTLEFFNTMVTPKVMHISNNGHTVLVRMTYEEGMEPRVRGGPLSELSPFTGYQFEQFHFHWGENDTVGSEDLIDNHAYPAELHVVLRNLEYPNFESALGKDHGLAVMAFFFQVKKEGTESYEDFTNQLAKIGRKGMSVNFTNPMPLRDYLSWDLLNYYTYVGSLTTPPCSEDVTWIDFMEPIDITEKQLDAFRQLTANDEHLNNNFRPIQPLNDRKVYKSVLEPMPLLNQSAIPFVNVENAANRRSYSPEVLLSGFGLVAALIASAFSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UD27</accession>
    <name>A0A6I8UD27_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell cycle checkpoint control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Rad9</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the rad9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353482.3">
        <property type="nucleotide sequence ID" value="XM_001353446.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UD27"/>
    <dbReference type="FunCoup" id="A0A6I8UD27">
        <property type="interactions" value="1250"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813559"/>
    <dbReference type="InParanoid" id="A0A6I8UD27"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UD27">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026584">
        <property type="entry name" value="Rad9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007268">
        <property type="entry name" value="Rad9/Ddc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04139">
        <property type="entry name" value="Rad9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009303">
        <property type="entry name" value="Cell_cycle_RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="397"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353482.3"/>
        </source>
    </evidence>
    <sequence length="458" mass="51186" checksum="0AF92D5792578A95" modified="2020-08-12" version="1">MKYTLEGSNARVIAKAVQSLSKVGKEMFIEIEQQGLQMRAINATQSAVGSIFFKRSMFEVFDMPPSSDFYCKISMKGCLAVFRNMNEVEYCELNLIDNQTNLQVNLRCKLETTKEATISIIDDQNINTNVDTDQRPNIIRGDHKLFMDISNNFNSAEEELTMEANTGSLVAKNYIEGARVNDKFMRTQLKLKPSEFDQYQVTKETIITFCIKEFRAFLLFAECLNTSLSLEFDDAGSPFMLKIKKHGEIECLLIMSTLSPDDVSFSDDYCQRDLTVEDEDVPAASSKRKSSTPGPSVQQKRLQQDGLVDIDSSMGTPLFQFRPSEAPSVQQARLLAEVDTVILIEDEAEEEALMLAAADAALEASQSVLPTTVPESAVAPAPPNSTEVCFYNTDDVPPTQSPTFPADDHLDDDESIPQSPERVTRMRTIFSRCFQSTYVPREPSPNSQAYAPNSDTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE92</accession>
    <name>A0A6I8UE92_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein argonaute 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4813746</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RAM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354040.2">
        <property type="nucleotide sequence ID" value="XM_001354004.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE92"/>
    <dbReference type="KEGG" id="dpo:4813746"/>
    <dbReference type="InParanoid" id="A0A6I8UE92"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UE92">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106005">
        <property type="term" value="P:RNA 5'-cap (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028271">
        <property type="entry name" value="RAMAC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48168">
        <property type="entry name" value="RNA GUANINE-7 METHYLTRANSFERASE-ACTIVATING SUBUNIT-LIKE (PSEUDOGENE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48168:SF1">
        <property type="entry name" value="RNA GUANINE-N7 METHYLTRANSFERASE ACTIVATING SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15320">
        <property type="entry name" value="RAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001354040.2"/>
        </source>
    </evidence>
    <sequence length="198" mass="21722" checksum="9AFEA3BEB64F5107" modified="2020-08-12" version="1">MVDPNRKINRQPVKDLNFLEECEAEFGSRYTEDDDEFMAHCSKPVAGPPIVGNWMSGGGGGGGGGRGYSGNNRYNGNRRGGGDRGWNRREGGGGGGYHHNNHDRGGYRDNHNRNRNRYDVYQRRDRGHHHGGGSRDGDGGYERSVGGGDDHHHGGGAGGGDRKRSFDQRSSRNDSQGRDQPPIKVRRDYGNFVPASKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UES9</accession>
    <name>A0A6I8UES9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Arginine-glutamic acid dipeptide repeats protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">LOC4814077</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354268.3">
        <property type="nucleotide sequence ID" value="XM_001354232.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042464.2">
        <property type="nucleotide sequence ID" value="XM_015186978.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237853.1">
        <property type="nucleotide sequence ID" value="XM_033381962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UES9"/>
    <dbReference type="KEGG" id="dpo:4814077"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072499">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UES9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052976">
        <property type="entry name" value="Scoloptoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933:SF40">
        <property type="entry name" value="CUTICULAR PROTEIN ANALOGOUS TO PERITROPHINS 1-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933">
        <property type="entry name" value="FI18007P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634433" evidence="2">
        <location>
            <begin position="26"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="130"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="546"/>
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        <location>
            <begin position="558"/>
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        <location>
            <begin position="594"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_033237853.1"/>
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    <sequence length="934" mass="104450" checksum="C3EC674407E57E17" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF98</accession>
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        <name evidence="6" type="primary">LOC4815278</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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                <consortium name="RefSeq"/>
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            <strain evidence="6">MV-25-SWS-2005</strain>
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    <sequence length="494" mass="54997" checksum="A5B2DF2A8599C37A" modified="2020-08-12" version="1">MGDAVEALFVSACSEENTTCSVQDLRTGTDLMKYKGGGNAQHHSLAMIGLNYVLAANTIKPLLHVWPINKQEQMSSLRFVLPGKANAMALTPDGAYLVVGIQESIYIWHMSTGRMLNTLSKHYQAITCLRFTDNGERFVSAGKDGAVLVWDLTFSVAPLDNGSQEGSEPLYSFNDHGLAVTDLHTGIGGIRSYLYTVSLDRCCKVYDLNGGIMLLSIVFPIALHSVVVNRMETSIYVGSAEGKSFVFHNWRAPRMKEYHLEEDDAQAFIGHTVGKPITSLALTMNAQLLVSGGEDKQVCVWDVASRQLMKSIPQNGVITNLRIRLLSPAIFQPQHKQPQLFANSLKRMITPPDVNDYIELLVTNEYPKARAKPKLNFDNCNLGPEDQKILQKMQHVNLGADDVDDDDDIEFDEEALKSTRQSSKVKDEDVALAVEEDFHKAHSNDPAVQKLIAENRRLKKKNEEVLQTMAKYLVDHSESGKGPPQRNRKKARKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIX8</accession>
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            <begin position="1"/>
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            <dbReference type="RefSeq" id="XP_001356213.4"/>
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    <sequence length="563" mass="64012" checksum="D47E666D52F39E49" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJI0</accession>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="8">MV-25-SWS-2005</strain>
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        <property type="component" value="Chromosome 4"/>
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    </dbReference>
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        <property type="entry name" value="Tscrpt_reg_GCM"/>
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    <dbReference type="PANTHER" id="PTHR12414">
        <property type="entry name" value="GLIAL CELLS MISSING RELATED/GLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12414:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR GLIAL CELLS MISSING-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03615">
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    </dbReference>
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    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GCM" evidence="6">
        <location>
            <begin position="32"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356375.3"/>
        </source>
    </evidence>
    <sequence length="519" mass="56581" checksum="CDFDA7AD7FDAC9B5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKQ5</accession>
    <name>A0A6I8UKQ5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NOL1/NOP2/Sun domain family member 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">l(2)10685</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a cytidine in rRNA + S-adenosyl-L-methionine = a 5-methylcytidine in rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15836"/>
            <dbReference type="Rhea" id="RHEA-COMP:15837"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357005.2">
        <property type="nucleotide sequence ID" value="XM_001356969.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKQ5"/>
    <dbReference type="FunCoup" id="A0A6I8UKQ5">
        <property type="interactions" value="395"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817603"/>
    <dbReference type="InParanoid" id="A0A6I8UKQ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKQ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000055">
        <property type="entry name" value="5-methylcytosine rRNA methyltransferase NSUN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808:SF3">
        <property type="entry name" value="5-METHYLCYTOSINE RRNA METHYLTRANSFERASE NSUN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808">
        <property type="entry name" value="NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="12">
        <location>
            <begin position="208"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001357005.2"/>
        </source>
    </evidence>
    <sequence length="504" mass="57745" checksum="F0AE21C3DEDFEB3B" modified="2020-08-12" version="1">MLILRNICWLQRRWKSGGKKRWNVLQNKKNNCDRALESFDDFYGSVYGGRWKNMRAALLTRHKYIAMVNNFGDTEQTCSMLETDGAINMKSLINLAQERLQGTDPALKGQGQNRYEIDDKLDALLRKQQEREVASIYPSAGEGGAAVQMPQQLKYDNVEEKEESQTESPDFKQSLTKALEEDVKLDEHRLVDPQFGTGGLYEYMPAHSIKGMEDWVAESEHYKYYQTSADFPLRIEPETSFHFPEHLALYTYEMGNCSDFKAPKKCLTGVSSHFLMDGASTLPPLFLQVQPGEKVLDACASPGGKSLLLLQTLHLDQLVCNDIQESRLNRLRRLMQEFLFDYKQRSAARHLIFSQSDARNLDEYEQYDKILVDVPCTTDRHVVMEQDNNIFKPSRIKERLRIPELQAGILTNCLRLLRPGGSLVYSTCSLSPIQNDGVVHMALQKVFVEHGITATIKDLSRHTELFSDIFKFEHPKGLKYGQMVIPYLPANFGPMYFSKITRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL97</accession>
    <name>A0A6I8UL97_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein teashirt</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">tsh</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the teashirt C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357271.3">
        <property type="nucleotide sequence ID" value="XM_001357235.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL97"/>
    <dbReference type="FunCoup" id="A0A6I8UL97">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817939"/>
    <dbReference type="InParanoid" id="A0A6I8UL97"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027008">
        <property type="entry name" value="Teashirt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487:SF7">
        <property type="entry name" value="PROTEIN TEASHIRT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487">
        <property type="entry name" value="TEASHIRT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="552"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="20"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="265"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="390"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="520"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="706"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="765"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="295"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="713"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="795"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="816"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="849"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="860"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="895"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="911"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="925"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001357271.3"/>
        </source>
    </evidence>
    <sequence length="996" mass="105731" checksum="7580E482A32A1D1A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULB3</accession>
    <name>A0A6I8ULB3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TEP1-F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Tep4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds covalently through a thioester bond to the pathogen surface resulting in pathogen clearance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of a TEP1-N chain and an TEP1-C chain non-covalently linked. Forms a complex composed of TEP1-N and TEP1-C heterodimer, LRIM1 and APL1C; the interaction stabilizes TEP1-N and TEP1-C heterodimer, prevents its binding to tissues while circulating in the hemolymph and protects the thioester bond from hydrolysis. Mature TEP1 and to a lesser extent full-length TEP1 interact with SPCLIP1; the interaction is induced by microbial infection.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357298.2">
        <property type="nucleotide sequence ID" value="XM_001357262.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8ULB3">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817973"/>
    <dbReference type="InParanoid" id="A0A6I8ULB3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000155">
        <property type="entry name" value="complement C3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.690:FF:000003">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="12">
        <location>
            <begin position="495"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="13">
        <location>
            <begin position="749"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="14">
        <location>
            <begin position="1370"/>
            <end position="1460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001357298.2"/>
        </source>
    </evidence>
    <sequence length="1479" mass="167122" checksum="4A161A204028F392" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULD3</accession>
    <name>A0A6I8ULD3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">General transcription factor 3C polypeptide 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4817991</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357329.2">
        <property type="nucleotide sequence ID" value="XM_001357293.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8ULD3">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817991"/>
    <dbReference type="InParanoid" id="A0A6I8ULD3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056467">
        <property type="entry name" value="eWH_GTF3C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044210">
        <property type="entry name" value="Tfc3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007309">
        <property type="entry name" value="TFIIIC_Bblock-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056428">
        <property type="entry name" value="WH_GTF3C1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04182">
        <property type="entry name" value="B-block_TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24101">
        <property type="entry name" value="WHD_GTF3C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23704">
        <property type="entry name" value="WHD_GTF3C1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="General transcription factor 3C polypeptide 1 winged-helix" evidence="8">
        <location>
            <begin position="9"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="B-block binding subunit of TFIIIC" evidence="7">
        <location>
            <begin position="176"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="GTF3C1 extended winged-helix" evidence="9">
        <location>
            <begin position="502"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1730"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1730"/>
            <end position="1745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357329.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1922" mass="221930" checksum="B8D505FF9953A945" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULJ6</accession>
    <name>A0A6I8ULJ6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-N-acetylglucosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">Naglu</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides.</text>
            <dbReference type="EC" id="3.2.1.50"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 89 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.50" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001357424.3">
        <property type="nucleotide sequence ID" value="XM_001357388.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236940.1">
        <property type="nucleotide sequence ID" value="XM_033381049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULJ6"/>
    <dbReference type="KEGG" id="dpo:4818063"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004561">
        <property type="term" value="F:alpha-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000107">
        <property type="entry name" value="Alpha-N-acetylglucosaminidase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.670">
        <property type="entry name" value="N-acetyl-b-d-glucoasminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007781">
        <property type="entry name" value="NAGLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024732">
        <property type="entry name" value="NAGLU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024240">
        <property type="entry name" value="NAGLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024733">
        <property type="entry name" value="NAGLU_tim-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12872">
        <property type="entry name" value="ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12872:SF1">
        <property type="entry name" value="ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05089">
        <property type="entry name" value="NAGLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12972">
        <property type="entry name" value="NAGLU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12971">
        <property type="entry name" value="NAGLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase N-terminal" evidence="10">
        <location>
            <begin position="44"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase tim-barrel" evidence="9">
        <location>
            <begin position="140"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase C-terminal" evidence="11">
        <location>
            <begin position="480"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001357424.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033236940.1"/>
        </source>
    </evidence>
    <sequence length="767" mass="88152" checksum="A9385E6330528DBC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULN5</accession>
    <name>A0A6I8ULN5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">COP9 signalosome complex subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CSN1a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357372.3">
        <property type="nucleotide sequence ID" value="XM_001357336.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULN5"/>
    <dbReference type="FunCoup" id="A0A6I8ULN5">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817955"/>
    <dbReference type="InParanoid" id="A0A6I8ULN5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF2">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="26S proteasome regulatory subunit Rpn7 N-terminal" evidence="7">
        <location>
            <begin position="129"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001357372.3"/>
        </source>
    </evidence>
    <sequence length="399" mass="45040" checksum="2DF0BA4E5C984C0E" modified="2020-08-12" version="1">MIAGTLEEAVDGASTSTGSTTVAAEPSTLHLATYASRYTDIARLNRLKFIAKVCPKLQVPALELAINHVKTFTYNVQLYDELYRTLCEAIEKREAEIEPSDEVVASTSKASSSSEAGCIKKAGRRVPYDAFWVEDNTMESTLLQQELDAELQYKKANSGSAKVRRILEEIGDYHLKGYNLKLAVKYYARARQYCTSTDNVINMFRNLIRVSIYMANWWHVLTYIEDAKKYALGFENLAQKVPPQLNCAAGLANMGLKIYKTAAQCFLQTPFDQYDFDKIVAREDVSYYVGLCGLATLDHDQLALDVINSVGFRPFFQLSPQVLSLVTTFHAGDFEHCLLVLNQIEDHVRLDYYLAPHVDTLFGKIKARIQKRRAPPAVPAAKAPQRLRVINERRNIRMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN00</accession>
    <name>A0A6I8UN00_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Chymotrypsin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4800764</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357979.3">
        <property type="nucleotide sequence ID" value="XM_001357942.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN00"/>
    <dbReference type="FunCoup" id="A0A6I8UN00">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800764"/>
    <dbReference type="InParanoid" id="A0A6I8UN00"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000047">
        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026007786" evidence="12">
        <location>
            <begin position="20"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="32"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001357979.3"/>
        </source>
    </evidence>
    <sequence length="280" mass="30814" checksum="7DAB8FDE0DA8CB29" modified="2020-08-12" version="1">MLPFLYVLTIVAGSCCVSGQVLYPPQYTNTRIVGGEEASEGVAPYQISLQGAASGQHSCGGAIIDERWIITAAHCTRGKQATSFRVLAGTQDLQQNATKYYYPDRIVEHCNYEPRKYRNDIALLHLNESIVFDNATQPVELDHEPLVPGSRLLLTGWGTLSLGGEVPARLQSLEVKYVPFEQCRAAHDNSTRVDIGHVCTFNDKGRGACHGDSGGPLVHNGRLVALVNWGMPCARGYPDAHASIAYYHDFIRTHLSLSETESAEGTPEEMKVLRSRRHYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNA0</accession>
    <name>A0A6I8UNA0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor SPF27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BCAS2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPF27 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358258.3">
        <property type="nucleotide sequence ID" value="XM_001358221.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNA0"/>
    <dbReference type="FunCoup" id="A0A6I8UNA0">
        <property type="interactions" value="1692"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801110"/>
    <dbReference type="InParanoid" id="A0A6I8UNA0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008409">
        <property type="entry name" value="SPF27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296">
        <property type="entry name" value="BCAS2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SPF27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05700">
        <property type="entry name" value="BCAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="209"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358258.3"/>
        </source>
    </evidence>
    <sequence length="284" mass="31568" checksum="9CB6A97B9E9C4086" modified="2020-08-12" version="1">MAGEVIVDALPYIDHGYDDVGVRESALAMVEEECRRYRPTKNYLDHLPLPATSPFETPLMVNEFERIQNRLPMETLSMKRYELPPPPSGKLGEVSAWQEAIENSMAQLEHQCVRSLNLELLLDYGTEAWKSYLEVFTAMQAKAQLQLQQLKKDIQDVNWQRKQAQTLAGERLRSLDAHWVLLVSKNYEIETECVELEKLVQAAREHIKTLGPTNANDTAPTPEHTNGHAEEDISESNGNINSNSSASGDGDGDVENVEDVRGGDGDANGDGDGDDAAEDESSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNC2</accession>
    <name>A0A6I8UNC2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Probable 28S rRNA (Cytosine-C(5))-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Nsun5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358184.2">
        <property type="nucleotide sequence ID" value="XM_001358147.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNC2"/>
    <dbReference type="FunCoup" id="A0A6I8UNC2">
        <property type="interactions" value="1453"/>
    </dbReference>
    <dbReference type="GeneID" id="4801011"/>
    <dbReference type="KEGG" id="dpo:4801011"/>
    <dbReference type="InParanoid" id="A0A6I8UNC2"/>
    <dbReference type="OMA" id="SFKSRIY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078968">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049561">
        <property type="entry name" value="NSUN5_7_fdxn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048889">
        <property type="entry name" value="NSUN5_RCM1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF4">
        <property type="entry name" value="28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21148">
        <property type="entry name" value="NSUN5_fdxn-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21153">
        <property type="entry name" value="NSUN5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="6">
        <location>
            <begin position="119"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358184.2"/>
        </source>
    </evidence>
    <sequence length="433" mass="48927" checksum="01A4EADE513CBCD1" modified="2020-08-12" version="1">MSKFTHSVKVPTQYKATAKILKVALEKQKSIKSLIFEEKHARVRSLQAVLKHYSDNRGAVDNAIEETSLLKDNPNLDSALAKVLVTELIFGRGQLNGDSKPVQTVREYKERLEKAISGSNIQKKEPNPRYVRVNTNLLSIAEALEYLSSEEWRRKELPAEATYADFLTAIRAMEEDEFMTDLHVEGVLIFHPKWAHYWAGHDFVHSKKFILQNKATSLAAELLAPPVGSTVLDLCAAPGMKTLHMCNVMQNKGRIYAVEQSQERYRALCDITNEAGCEIVTPILGDALTMDATRCPDVEYILVDPSCSGNGMQSRISVCDEQKDDKRLYKLAGLQIKILSHAMSAFPNVKRIAYCTCSLWKEENEQVVQRCLQLNPSFKMLSCKKALRNKWQNVGDKEYAKIGKNCLYCLPDSDLTDGIFLALFEKKRDGEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPF2</accession>
    <name>A0A6I8UPF2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Mccc1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA. Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358754.5">
        <property type="nucleotide sequence ID" value="XM_001358717.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPF2"/>
    <dbReference type="FunCoup" id="A0A6I8UPF2">
        <property type="interactions" value="1777"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801707"/>
    <dbReference type="InParanoid" id="A0A6I8UPF2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000028">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF33">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0092">Biotin</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="13">
        <location>
            <begin position="29"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="148"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="11">
        <location>
            <begin position="619"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358754.5"/>
        </source>
    </evidence>
    <sequence length="702" mass="76382" checksum="C94953ED06B3A907" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPH7</accession>
    <name>A0A6I8UPH7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hamartin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Tsc1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358813.2">
        <property type="nucleotide sequence ID" value="XM_001358776.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPH7"/>
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        <property type="interactions" value="1868"/>
    </dbReference>
    <dbReference type="GeneID" id="4801772"/>
    <dbReference type="KEGG" id="dpo:4801772"/>
    <dbReference type="InParanoid" id="A0A6I8UPH7"/>
    <dbReference type="OMA" id="NRMASYS"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079388">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UPH7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007483">
        <property type="entry name" value="Hamartin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154">
        <property type="entry name" value="HAMARTIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154:SF2">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04388">
        <property type="entry name" value="Hamartin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="596"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1082"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="739"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="910"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1088"/>
            <end position="1107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358813.2"/>
        </source>
    </evidence>
    <sequence length="1107" mass="126127" checksum="D5DA1DD423B38673" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPR4</accession>
    <name>A0A6I8UPR4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mucin-5AC-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801727</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358771.2">
        <property type="nucleotide sequence ID" value="XM_001358734.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPR4"/>
    <dbReference type="GeneID" id="4801727"/>
    <dbReference type="KEGG" id="dpo:4801727"/>
    <dbReference type="InParanoid" id="A0A6I8UPR4"/>
    <dbReference type="OMA" id="ANNADCT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026209065" evidence="2">
        <location>
            <begin position="25"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358771.2"/>
        </source>
    </evidence>
    <sequence length="155" mass="16454" checksum="A7F8BDC9CDEF5C91" modified="2020-08-12" version="1">MKVSSNIFALAIVLFACLLRSSEAVTCTADPTVTGCIDCTTSPTDAECVAEAAAATTSTVAPVVSTTAATATTVATDTATTTTAASTTSTGGRRKVVRISNLRYTAVRRIRVNRSGTTSRTTSRNTNTNRNRNLNRRRVNVRNRRRNGNVRVIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPR7</accession>
    <name>A0A6I8UPR7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloendopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4801919</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001358937.3">
        <property type="nucleotide sequence ID" value="XM_001358900.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPR7"/>
    <dbReference type="GeneID" id="4801919"/>
    <dbReference type="KEGG" id="dpo:4801919"/>
    <dbReference type="InParanoid" id="A0A6I8UPR7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070275">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000042">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF859">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="111"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358937.3"/>
        </source>
    </evidence>
    <sequence length="333" mass="38122" checksum="492166DBE4C8A9A7" modified="2020-08-12" version="1">MEIVTAKKNQNPRRIIMCAQLSGVVVVVLVLVMASPPSSESAKLPTRQYPAGVDFFEHEFSSVAADEYDPYGPDIGNDDLGIDDPETMPRLFQGDIAIDPYTYITLRLGVNPMRHPKRLWPNGTIPFEISPRYVNEEREAIVQALKTFNSLTCIKFVPYDGEVEDYLLIEPPEEGPKGCWSYVGKRGGEQIVSLQRPDETSAHCFSSEGRIMHELMHAIGIYHEQSRADRDNFVKIHWENIVPRFRKNFKLISRKKGKYAFDYDYNSVMHYGEFYFSKKKGQKPTMTPLQPGVRIGQRKTISKIDCLKINELYGCLQGRRAKMYRGFCHLLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPW7</accession>
    <name>A0A6I8UPW7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein C19orf12 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Nazo</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C19orf12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358833.3">
        <property type="nucleotide sequence ID" value="XM_001358796.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPW7"/>
    <dbReference type="KEGG" id="dpo:4801799"/>
    <dbReference type="InParanoid" id="A0A6I8UPW7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033369">
        <property type="entry name" value="C19orf12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31493">
        <property type="entry name" value="NAZO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31493:SF1">
        <property type="entry name" value="PROTEIN C19ORF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20721">
        <property type="entry name" value="C19orf12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358833.3"/>
        </source>
    </evidence>
    <sequence length="140" mass="14856" checksum="CAA9903303382D75" modified="2020-08-12" version="1">MPIDTGEIIKALAILADRRNIQVTFKETAKGAALCAAGAVIGGLLMGSRGLAVGGAIGSLAAYRLTEGKFKSLREIIMDDLTESQRIELEQHVINAVANVGYEDVLSIGALIMSNRRVQDIAMNVLKSFATEQLGLSIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ92</accession>
    <name>A0A6I8UQ92_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Xylulose kinase</fullName>
            <ecNumber evidence="4">2.7.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4802087</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Phosphorylates D-xylulose to produce D-xylulose 5-phosphate, a molecule that may play an important role in the regulation of glucose metabolism and lipogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-xylulose + ATP = D-xylulose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17140"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.17" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001359074.2">
        <property type="nucleotide sequence ID" value="XM_001359037.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ92"/>
    <dbReference type="FunCoup" id="A0A6I8UQ92">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="GeneID" id="4802087"/>
    <dbReference type="KEGG" id="dpo:4802087"/>
    <dbReference type="InParanoid" id="A0A6I8UQ92"/>
    <dbReference type="OMA" id="NSCALGG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077518">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UQ92">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004856">
        <property type="term" value="F:D-xylulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005997">
        <property type="term" value="P:xylulose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07776">
        <property type="entry name" value="ASKHA_NBD_FGGY_SpXK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000118">
        <property type="entry name" value="Xylulose kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042024">
        <property type="entry name" value="D-XK_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF57">
        <property type="entry name" value="XYLULOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0859">Xylose metabolism</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="5">
        <location>
            <begin position="132"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="6">
        <location>
            <begin position="297"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359074.2"/>
        </source>
    </evidence>
    <sequence length="550" mass="60404" checksum="56AC6C5CD5A808B6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQM6</accession>
    <name>A0A6I8UQM6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359263.2">
        <property type="nucleotide sequence ID" value="XM_001359226.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQM6"/>
    <dbReference type="KEGG" id="dpo:4802323"/>
    <dbReference type="InParanoid" id="A0A6I8UQM6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359263.2"/>
        </source>
    </evidence>
    <sequence length="192" mass="21868" checksum="D8C52B149C8E0EB3" modified="2020-08-12" version="1">MSIEDRLICPHLLRGRSPNSIAYSMNPNWFHQEDLLRSVYSEQDLQSECQIMGLMMSVSRSRRSLHPSVLRRVGGMDNKTQPEPTDADVVFGICDKSPPELRCGWGKSRIEWLWQLERQKKLNQAWLEPCGSIDLARSGSRTSTDIRGKNKGKSRRHQPGCWSWMRHSCGLGLDSGSLDSLDVPYCCRETSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URW0</accession>
    <name>A0A6I8URW0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Ugt302E1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001359926.3">
        <property type="nucleotide sequence ID" value="XM_001359889.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URW0"/>
    <dbReference type="FunCoup" id="A0A6I8URW0">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803134"/>
    <dbReference type="InParanoid" id="A0A6I8URW0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000144">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026379664" description="UDP-glucuronosyltransferase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="476"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359926.3"/>
        </source>
    </evidence>
    <sequence length="512" mass="57999" checksum="11A6FEA84B218503" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USU0</accession>
    <name>A0A6I8USU0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Tim10</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Peripheral membrane protein</topology>
            <orientation evidence="1 14">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360366.1">
        <property type="nucleotide sequence ID" value="XM_001360329.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USU0"/>
    <dbReference type="SMR" id="A0A6I8USU0"/>
    <dbReference type="FunCoup" id="A0A6I8USU0">
        <property type="interactions" value="1623"/>
    </dbReference>
    <dbReference type="GeneID" id="4803689"/>
    <dbReference type="KEGG" id="dpo:4803689"/>
    <dbReference type="InParanoid" id="A0A6I8USU0"/>
    <dbReference type="OMA" id="ESCYSKC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082076">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="15">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001360366.1"/>
        </source>
    </evidence>
    <sequence length="92" mass="10536" checksum="A82525FFAC6F9119" modified="2020-08-12" version="1">MDMSQISPADQAKVQVMQEMEIEMMSDLYNRMTTACHKKCIPPRYSESELGKGEMVCIDRCVAKYLDIHEHIGKKLTAMSMQDAELMKNMTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTA0</accession>
    <name>A0A6I8UTA0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox protein engrailed-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">inv</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Engrailed homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360550.3">
        <property type="nucleotide sequence ID" value="XM_001360513.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTA0"/>
    <dbReference type="FunCoup" id="A0A6I8UTA0">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803906"/>
    <dbReference type="InParanoid" id="A0A6I8UTA0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000345">
        <property type="entry name" value="Homeobox protein engrailed-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050720">
        <property type="entry name" value="Engrailed_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000747">
        <property type="entry name" value="HD_engrailed"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019549">
        <property type="entry name" value="Homeobox-engrailed_C-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019737">
        <property type="entry name" value="Homeobox-engrailed_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24341">
        <property type="entry name" value="HOMEOBOX PROTEIN ENGRAILED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24341:SF6">
        <property type="entry name" value="HOMEOBOX PROTEIN INVECTED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10525">
        <property type="entry name" value="Engrail_1_C_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00026">
        <property type="entry name" value="ENGRAILED"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00033">
        <property type="entry name" value="ENGRAILED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="519"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="521"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="287"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="356"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="480"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="509"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU510713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001360550.3"/>
        </source>
    </evidence>
    <sequence length="626" mass="67151" checksum="E6C5EF2B6D82CE5A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTE6</accession>
    <name>A0A6I8UTE6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sentrin-specific protease 8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Den1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360346.3">
        <property type="nucleotide sequence ID" value="XM_001360309.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTE6"/>
    <dbReference type="FunCoup" id="A0A6I8UTE6">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="GeneID" id="4803663"/>
    <dbReference type="KEGG" id="dpo:4803663"/>
    <dbReference type="InParanoid" id="A0A6I8UTE6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081104">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UTE6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="61"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001360346.3"/>
        </source>
    </evidence>
    <sequence length="259" mass="29930" checksum="EED7D2511C57DAC3" modified="2020-08-12" version="1">MLPQDRKILRQHTRQNQSSNQTGASRRVGGKSGTPNRYNNHHSKMSANSKADPISLSFHDSCLRMSDVQLLHGPRWLNDQILSFYYEYLTHVKYKTNDDIVFIAPEVTQCMKYMDDQELEQLMDENKATRKPFIFFALNDNMTFEAGGTHWSLLVFSRPEKTFYHFDSYGNNNTSNSVELMNKVKETMDMRSAKFRPMRCLQQANGYDCGIHVICMTDHIADYLNRYEVIDGLPPLQVDTVKAKRTELLKLILSLGGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU40</accession>
    <name>A0A6I8UU40_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Programmed cell death protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Zfrp8</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298">
        <property type="entry name" value="PCDC2 PROGRAMMED CELL DEATH PROTEIN 2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12298:SF4">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDCD2_C"/>
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        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HIT/MYND zinc finger-like"/>
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        <property type="entry name" value="ZF_MYND_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="128"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360905.3"/>
        </source>
    </evidence>
    <sequence length="348" mass="39283" checksum="BED1B9A55565A8F1" modified="2020-08-12" version="1">MEIDLGFAEKRDNNGWLTNRYFPSKLGGLPAWLELEALPDPKQLQCQKCMAPKVFLAQLYAPHEDDFNFHRSIYIFVCRNADCQQDHSAENFTVLRSQLALKNKFFSEQAAEDDGEPLPPIPCLKKVCAACGCYAPHACSRCKTIYYCSSAHQRAHWSDHKANCGSSNAVPHKPLKAIEFEEFEIVIESNPTRPVEEDHKDDDARLAEFEALQASGNTGDLSNVSEAEMDKYFGNTAGSDDKTFRQFKKQIADEPEQIVRYKRGGEPLWITNTAATVADQLKSLPNCSQCGGIRQFEFQIMPQTLVLLKEEFLDWGVLAVYTCAKSCPIEGYVEEHMVKQDVVSQENH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUT5</accession>
    <name>A0A6I8UUT5_DROPS</name>
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        <submittedName>
            <fullName evidence="12">Mitochondrial sodium/calcium exchanger protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4804735</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361248.3">
        <property type="nucleotide sequence ID" value="XM_001361211.4"/>
    </dbReference>
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        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804735"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UUT5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:calcium:sodium antiporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
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    </dbReference>
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        <property type="entry name" value="NCX, central ion-binding region"/>
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    </dbReference>
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        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="515"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="72"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="381"/>
            <end position="532"/>
        </location>
    </feature>
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        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361248.3"/>
        </source>
    </evidence>
    <sequence length="546" mass="62549" checksum="C1671DF260770F13" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVS4</accession>
    <name>A0A6I8UVS4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">U3 small nucleolar RNA-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">U3-55K</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361669.3">
        <property type="nucleotide sequence ID" value="XM_001361632.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVS4"/>
    <dbReference type="FunCoup" id="A0A6I8UVS4">
        <property type="interactions" value="1678"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805232"/>
    <dbReference type="InParanoid" id="A0A6I8UVS4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000509">
        <property type="entry name" value="U3 small nucleolar RNA-interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039241">
        <property type="entry name" value="Rrp9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19865:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="255"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="297"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="338"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361669.3"/>
        </source>
    </evidence>
    <sequence length="487" mass="53832" checksum="4BD17A74DA67D67E" modified="2020-08-12" version="1">MSSSFFLKAGGKANKKRKNPAATKGKGVKTAAKSGKSGFQNARAEGPVPKKQRRPKEKDEEIESDDEEYNSDLGDGGHNTLAFSSDEGEEETAQDKRLRLAKQYLSEIEKQESERAEDRELGQSVEQRLQTDYLDSVGKLRRNIAGNLKTNDVTCVLKHKLHHMPICALALSPDGKFLYAGAKSQFVLKWSTQNGKVLDKCDVLPHREEPETGKKRRSHVIAICLSTDMKYMALAEGGPHIQIWCPQTMKHVKTLKGHRDSVAGLVFRKGTHDLYSAAKDRSVKIWSLDEMAYVESLFGHQTGVTSIDALSRERAITAGGSDCSLRIWKITEESQLIYNGHRDSIECVKLMNDEHFVSGGVDGAISLWSALKKKPICTTQLAHGVGENGVANWITAIAVVVNTDLVATGSCDGFVKLWQTNPNARKLQQIQSIPITGFINGLTFNADGTKLYVGVGQEHRLGRWWRHKEAKNNVVVVDIVLQNATGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX01</accession>
    <name>A0A6I8UX01_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4485 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6903452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132331.2">
        <property type="nucleotide sequence ID" value="XM_002132295.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX01"/>
    <dbReference type="KEGG" id="dpo:6903452"/>
    <dbReference type="InParanoid" id="A0A6I8UX01"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027831">
        <property type="entry name" value="DUF4485"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14846">
        <property type="entry name" value="DUF4485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4485" evidence="2">
        <location>
            <begin position="82"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002132331.2"/>
        </source>
    </evidence>
    <sequence length="477" mass="54915" checksum="DF49283290D7D868" modified="2020-08-12" version="1">MFSPKKFDDRWVSHNKETDERSRQSRPGSSRGSRNTMTERADGRNKSEPGTVYDASVNLNISDCLEMLESSPQPEEAFHPNSRECHCARAWLNALTSMQCNTVMEACLRNGYISALSVCLNQKILYGIFEQMPPEELNWMDILDTTTNASSIHVSFRSNEALRQQFVPFESMNLSSLPSLSVQEEMVRQQRFIEEPQVFRAEELQRFQQERQRYQEQPPQTETFYDPDTFPPPPHLRQQSGAYYKPSSVKFYSFGAAPRFQAANQEGADSCPSGAEGGGLATAVVGPTRSILRRKCFSAQVPRVYPSYLKTPLSFNARRSVDDEIWYTGPIQSYRMKFPSQEYVFKGSKQFKRSKQVLQNAPGTQTNHWKPEAIEEDSDREKTGQTVISRSQCDMSLLRECIRRELRGESPVGANDFLEAEIVRYKSINSRYRRDDPEYKEKMLAGDPKTQRTYLLLNMEKDLTSYYAVQRQRKVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY44</accession>
    <name>A0A6I8UY44_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Perlucin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133108.2">
        <property type="nucleotide sequence ID" value="XM_002133072.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY44"/>
    <dbReference type="KEGG" id="dpo:6902580"/>
    <dbReference type="InParanoid" id="A0A6I8UY44"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UY44">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026147236" evidence="1">
        <location>
            <begin position="21"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="33"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133108.2"/>
        </source>
    </evidence>
    <sequence length="180" mass="20321" checksum="EE2C0CDEF42B8AC2" modified="2020-08-12" version="1">MAHLLKIFLWLFGLFTAALSQVKIGCPPGFTLIEEKCYYVSLEKENWFNAHRRCFTLNSTLVVFDDEKDMKVVTASLKVLGLPFSNGWTDSIWTGITSLGTNGHFVLSHDGGPLAYAQWAPNQPNNATAHECVAYANNNGFGYHNTQCDFHQFPFVCQAKKIESESYLCLKKNQFVEIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYF7</accession>
    <name>A0A6I8UYF7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Alpha-mannosidase</fullName>
            <ecNumber evidence="10">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LManV</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_002133315.2">
        <property type="nucleotide sequence ID" value="XM_002133279.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYF7"/>
    <dbReference type="FunCoup" id="A0A6I8UYF7">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902369"/>
    <dbReference type="InParanoid" id="A0A6I8UYF7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10810">
        <property type="entry name" value="GH38N_AMII_LAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000018">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1360:FF:000004">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026375784" description="Alpha-mannosidase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="11">
        <location>
            <begin position="358"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="2002"/>
            <end position="2012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="2013"/>
            <end position="2022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002133666.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015041180.2"/>
        </source>
    </evidence>
    <sequence length="2133" mass="225683" checksum="900A5FDB1550A367" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYX6</accession>
    <name>A0A6I8UYX6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133670.2">
        <property type="nucleotide sequence ID" value="XM_002133634.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYX6"/>
    <dbReference type="KEGG" id="dpo:6902139"/>
    <dbReference type="InParanoid" id="A0A6I8UYX6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133670.2"/>
        </source>
    </evidence>
    <sequence length="120" mass="13785" checksum="7093117AA64A9E99" modified="2020-08-12" version="1">MDTKEKPQWETDSKPQPQVETQTELLSQQQSQQLTDGLVETTIPTRRLRRLGPPMPPDDMLRLSIADNLMQIVGNMTNDEYQRQCCEIFQVSELTHGVHGQILAALQHIDEQRQRPGGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYZ8</accession>
    <name>A0A6I8UYZ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2-22 kDa-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133731.2">
        <property type="nucleotide sequence ID" value="XM_002133695.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYZ8"/>
    <dbReference type="KEGG" id="dpo:6902204"/>
    <dbReference type="InParanoid" id="A0A6I8UYZ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="11"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133731.2"/>
        </source>
    </evidence>
    <sequence length="211" mass="24187" checksum="82B121D969FB60D4" modified="2020-08-12" version="1">MWSNTGAARVQREINTAMHMRSIGGETTERGFKLWLVDQSWSNLCVEIHGPPDSPYEGGVFVVKILVPDTYPVVPPQVEFRTRIWHPNISPESGAISLDILWKGWSPDMSIRTILMTVRNIIAIPDMGTPFDDDVARQYIHKHEMFVRTAKHWTNRYASGPNIIPEFDEKIQVLKDLGMYECLARSILSKKDWDVGKVSHNFLTIKSQHEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V183</accession>
    <name>A0A6I8V183_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibrous sheath CABYR-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135428.3">
        <property type="nucleotide sequence ID" value="XM_002135392.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V183"/>
    <dbReference type="KEGG" id="dpo:6900064"/>
    <dbReference type="InParanoid" id="A0A6I8V183"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033234">
        <property type="term" value="P:negative regulation of protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043375">
        <property type="entry name" value="FSCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004011">
        <property type="entry name" value="Gyr_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004019">
        <property type="entry name" value="YLP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36135">
        <property type="entry name" value="FIBROUS SHEATH CABYR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36135:SF1">
        <property type="entry name" value="FIBROUS SHEATH CABYR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02756">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02757">
        <property type="entry name" value="YLP"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00713">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026298087" description="Fibrous sheath CABYR-binding protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="1144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135428.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1144" mass="128741" checksum="0F62276C114129FE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2D9</accession>
    <name>A0A6I8V2D9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137346.3">
        <property type="nucleotide sequence ID" value="XM_002137310.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2D9"/>
    <dbReference type="KEGG" id="dpo:6897166"/>
    <dbReference type="InParanoid" id="A0A6I8V2D9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026231390" evidence="1">
        <location>
            <begin position="31"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137346.3"/>
        </source>
    </evidence>
    <sequence length="128" mass="15043" checksum="9588003295106760" modified="2020-08-12" version="1">MWQSVTMDRKLIFVVPLLLWQLVHIPGSTGGYSGFEIAQEITTVRQYLPGPIRLQRTEGRTRNTLSIDQENATFNGEMVDNYCCFWVYQKHPAIPENWEHRAEYPWDFVLNGIFVKNQRNYTDPDPVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2H1</accession>
    <name>A0A6I8V2H1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phospholipase A1 VesT1.02-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6897057</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137241.2">
        <property type="nucleotide sequence ID" value="XM_002137205.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2H1"/>
    <dbReference type="ESTHER" id="drops-q29ad9">
        <property type="family name" value="Insect_lipase"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897057"/>
    <dbReference type="InParanoid" id="A0A6I8V2H1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000076">
        <property type="entry name" value="phospholipase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002334">
        <property type="entry name" value="Allerg_PlipaseA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF150">
        <property type="entry name" value="FI01825P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00825">
        <property type="entry name" value="DOLALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026274240" evidence="5">
        <location>
            <begin position="17"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="56"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137241.2"/>
        </source>
    </evidence>
    <sequence length="339" mass="36760" checksum="CEB90E53E9381863" modified="2020-08-12" version="1">MKVSFVLAAILAAGSALPIEERINGENGWYVPQADGSFEWMELKDAEELLANAEVTDGRISTNDVSFYLYTKSNPTDGKEIKAKASSVDASHFNKDHGIRFVIHGWTGRYTDDMNTRITKSWLSKGDYNVIIVDWARARSVDYASSVIAVPGAGGKVGEMINYLHEHHGMSLDSLEVIGHSLGAHVSGYAGKTVGKGRIHSIVGLDPALPLFSYDKPDKRLSSDDAHYVESIQTNGGKLGFLKPIGKGAFYPNGGKKQPGCGVDVTGSCSHGRSVLYYAEAVTEDNFGTIRCHDYEAAVSKECGSTYSSVRMGAVHNAYMVEGDFYVPVNSKEPFGKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2Q5</accession>
    <name>A0A6I8V2Q5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase COP1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6897235</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137407.2">
        <property type="nucleotide sequence ID" value="XM_002137371.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2Q5"/>
    <dbReference type="KEGG" id="dpo:6897235"/>
    <dbReference type="InParanoid" id="A0A6I8V2Q5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047134">
        <property type="entry name" value="RNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041:SF78">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="156"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="76"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137407.2"/>
        </source>
    </evidence>
    <sequence length="209" mass="22718" checksum="6BB37CB4A528D426" modified="2020-08-12" version="1">MSSENLQVSSSTGTSTQSSQSTPNASVSSNLSDGENSGSRAAPPMVTAGGILNRIVSGIRSAYLEILARIPMVVPRNNRSDSTSNRAPEAAIGLRTPNSLSTDRIRAPSTSRRRRHASTNTATTGPTASDAEVSGPSRPKRQRTDSNESLEETYNCPICWESVSNRDPVATKCGHIFCRQCIRTAIRRFHKCPLCRMRLTLRQLKPITM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V313</accession>
    <name>A0A6I8V313_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">F-box/WD repeat-containing protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">slmb</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137670.3">
        <property type="nucleotide sequence ID" value="XM_002137634.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V313"/>
    <dbReference type="FunCoup" id="A0A6I8V313">
        <property type="interactions" value="1902"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897521"/>
    <dbReference type="InParanoid" id="A0A6I8V313"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248716">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V313">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000001">
        <property type="entry name" value="F-box/WD repeat-containing protein 11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000004">
        <property type="entry name" value="F-box/WD repeat-containing protein 11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021977">
        <property type="entry name" value="Beta-TrCP_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050995">
        <property type="entry name" value="WD-F-box_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604:SF4">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12125">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01028">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="98"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="206"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="246"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="286"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="329"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="369"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="409"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="458"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137670.3"/>
        </source>
    </evidence>
    <sequence length="510" mass="58855" checksum="7969A5425D685A93" modified="2020-08-12" version="1">MMKMDTDKIMDETNSNAQAFTTTMLYDPVRKKDSSPTYQTERELCFQYFTQWSESGQVDFVEQLLSRMCHYQHGQINAYLKPMLQRDFITLLPIKGLDHIAENILSYVDAESLKSAELVCKEWLRVISEGMLWKKLIERKVRTDSLWRGLAERRNWMQYLFKPRPGQTQRPHSFHRELFPKIMNDIDSIENNWRTGRHMLRRINCRSENSKGVYCLQYDDGKIVSGLRDNTIKIWDRTDLQCVKTLTGHTGSVLCLQYDDKVIISGSSDSTVRVWDVNTGDMVNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMTSPSEITLRRVLVGHRAAVNVVDFDEKYIVSASGDRTIKVWSTSSCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNSIRLWDIECGACLRVLEGHEELVRCIRFDTKRIVSGAYDGKIKVWDLVAALDPRAASNTLCLNTLVEHTGRVFRLQFDEFQIVSSSHDDTILIWDFLNFTPNENKTGRTPSPALMEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3P7</accession>
    <name>A0A6I8V3P7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase 1 regulatory subunit 36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6897977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138076.2">
        <property type="nucleotide sequence ID" value="XM_002138040.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3P7"/>
    <dbReference type="KEGG" id="dpo:6897977"/>
    <dbReference type="InParanoid" id="A0A6I8V3P7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026142">
        <property type="entry name" value="Pro_pase_1_reg_su_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21055">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21055:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14895">
        <property type="entry name" value="PPPI_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002138076.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="54574" checksum="D026CFD9352773DA" modified="2020-08-12" version="1">MLRLHVGKNAPKYYKGNWSFDSKKDALNFEPYHDLEDVEERYISTNSFNFLRTITQEEELVFRHVYGRDDTTYDTRAVELNDIRDLVLFLMPSEFLRPKFVEFMHHQAVYHLLHSLTIYFEYYLRMVEFVLIRRDEISGETAQIQNEQTNDVKRVFSVYLSQYRMLVARNYSVIIKGEGDLEKFYHMKKLVNISQSIWDKNFHEQLLACSTQIVWIAMHRRAYNVIEMEMNRLFCSEHFVTARAGYPIFTPAEKSLLYGRNSRIMNYRQQVSPLIQELQKLNNEDLPILWIGKRKYRGTDIRIMEMELEYIVPGPQLRLIDVAHGILGHPRKLYNTILELHWPSVRYSNFSKHHDPYFIVRQPLLKIPKMDEMKRRQMAETYESFYKIKEINDHPSRAIYLKWVKRDKLINDYRSGVLLTSTLLRAENELNSTSLRPNVDQIIADYKKSVAKNRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3S0</accession>
    <name>A0A6I8V3S0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Neuropeptide CCHamide-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CCHa1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137446.3">
        <property type="nucleotide sequence ID" value="XM_002137410.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3S0"/>
    <dbReference type="FunCoup" id="A0A6I8V3S0">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897286"/>
    <dbReference type="InParanoid" id="A0A6I8V3S0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037729">
        <property type="entry name" value="CCHa1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35980">
        <property type="entry name" value="NEUROPEPTIDE CCHAMIDE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35980:SF1">
        <property type="entry name" value="NEUROPEPTIDE CCHAMIDE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026301206" evidence="2">
        <location>
            <begin position="23"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137446.3"/>
        </source>
    </evidence>
    <sequence length="213" mass="23910" checksum="BF31841FEE85B9D9" modified="2020-08-12" version="1">MWYMKCSWTLLVFVALFALVSGSCLEYGHSCWGAHGKRSGGKAAVAAKAGGNPLAVETLAEQLYNNNQNNNQNIREDTDDDTNNTNNISSLNNMPPRAPALSAQSDDPEKRNRNHRNHEAKDLKWAQLMRQQHRYQLRQQQQQQKQSRLWAGGRCGGQYDAAAESWRKLQQALILAQAQAQAQAQVEAEAEGEAEAEAEQQQQTLSELYELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V465</accession>
    <name>A0A6I8V465_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 808-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6897580</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137725.4">
        <property type="nucleotide sequence ID" value="XM_002137689.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V465"/>
    <dbReference type="KEGG" id="dpo:6897580"/>
    <dbReference type="InParanoid" id="A0A6I8V465"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF114">
        <property type="entry name" value="KLUMPFUSS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="12">
        <location>
            <begin position="18"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="332"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="89"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="211"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002137725.4"/>
        </source>
    </evidence>
    <sequence length="406" mass="46730" checksum="67D9F6C67EB77D5B" modified="2020-08-12" version="1">MRANLFSLLINKNRKMSEQCRCCANAICGTTANIFQMENIEMLEVLQAVSGINFVNDPGLPSCICVYCLHDLDQTIAFRNRIRTQANLLQQQRRGKSPSKISKWHMPNVDEDKPSDDVHVSFDGKEDLSYGFPESHENSDSLALRLKEEPHDEENKQPIYADDRDSPMRSRLKELEENNFGDQTPDADYNAQVQVDDSLIASLKKELEEVDFTESDAEKTSQPRKLSNRGSNSNSPTSLLTESKQRSLAEKPIECAVLKKKKKRRTIEKSFKCKACSEQFLTPGIRNRHERVSCPVIMETLTDDKRFKCKHCSKHFSSSGNRKHHERTHLTFSCEICGTAFRYKYWLKLHLSKVHNQTGEKIFQCDPCEKTFSRNHQLERHLATIVHQQAVNGPNAIRKKRYTTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4Q8</accession>
    <name>A0A6I8V4Q8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Probable protein transport protein Sec61 subunit gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6898577</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138597.3">
        <property type="nucleotide sequence ID" value="XM_002138561.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4Q8"/>
    <dbReference type="KEGG" id="dpo:6898577"/>
    <dbReference type="InParanoid" id="A0A6I8V4Q8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002138597.3"/>
        </source>
    </evidence>
    <sequence length="84" mass="9651" checksum="DA34B9B6EBAEB1E4" modified="2020-08-12" version="1">MCKDFQQMSWGPSGRPKFDFMVLLTVPFITDGLRFFKRCHKPDRREFKRTAVAAGVGILLMGLVGYIVKLLQIPLILSSYQNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V506</accession>
    <name>A0A6I8V506_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Tsp42Ei</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138180.1">
        <property type="nucleotide sequence ID" value="XM_002138144.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V506"/>
    <dbReference type="SMR" id="A0A6I8V506"/>
    <dbReference type="FunCoup" id="A0A6I8V506">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="6898098"/>
    <dbReference type="KEGG" id="dpo:6898098"/>
    <dbReference type="InParanoid" id="A0A6I8V506"/>
    <dbReference type="OMA" id="GCKSQFV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246012">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF551">
        <property type="entry name" value="RE08073P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="139"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002138180.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="24789" checksum="B5BC9FD3E165B711" modified="2020-08-12" version="1">MGLASTTVKHLLLLLNFVFAVLGLVLIAFGIFFLISAAENAVSIGENVAGGLIIGLGAVILVIAVFGCLAAIHESPVRLLIYVGVVVVLILTQLLFLSMASHGTKDGLSGSINEGFDRLWEAERNESGALAYYENWLHCCGVNSSEDYWIIHHAIPSSCCLDNKCVDSTSVYKVGCKAEFVEYLDDKLLVFKIVCWLLIIGEAVGAVFGWLLYSSVKNQSRRNNAVWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V562</accession>
    <name>A0A6I8V562_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein 724-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC13036407</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_003736154.2">
        <property type="nucleotide sequence ID" value="XM_003736106.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V562"/>
    <dbReference type="KEGG" id="dpo:13036407"/>
    <dbReference type="InParanoid" id="A0A6I8V562"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V562">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000870">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_003736154.2"/>
        </source>
    </evidence>
    <sequence length="398" mass="46368" checksum="06A077070D4244C9" modified="2020-08-12" version="1">MSYSTCNTCGKQFAKPTFLRRHQVVHTKEKSYACETCSLCFSQQSSLQRHKRIKHTRAEASPVLGDADQVAHQALSALLDLQSGMESLVRPQNSPTQNSMQTTMEEHPQCRIVSVLDRLDPSGQLTTLTVQRIQRSPNKCFYVCEFCAKEFFKSYDLIRHRRSHTKERPYPCVTCLRRFSTRTKLNEHQKRMHRKSSNCQLNEKKQRKMERGQKLAELQQLLPCPQQSITSGKSYKCVYCSRIYKRKFNCRRHMLTHLSRFLNEKQVNPHCRKNSSPNRCLECRKPFKNTNDLSRHLLTHTNTKSHVCHLCHKAFNLKSTLSRHLVTHLKSSQRPSVNCQVCGKLYGSKSALQIHLRLHTGERPFKCEICQQTFRTSGHRIEHMRAERHRALPPSSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5P1</accession>
    <name>A0A6I8V5P1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphorylated adapter RNA export protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNA U small nuclear RNA export adapter protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Phax</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PHAX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138568.2">
        <property type="nucleotide sequence ID" value="XM_002138532.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5P1"/>
    <dbReference type="FunCoup" id="A0A6I8V5P1">
        <property type="interactions" value="1438"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898533"/>
    <dbReference type="InParanoid" id="A0A6I8V5P1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006408">
        <property type="term" value="P:snRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1440:FF:000001">
        <property type="entry name" value="phosphorylated adapter RNA export protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1440">
        <property type="entry name" value="PHAX RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039047">
        <property type="entry name" value="PHAX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019385">
        <property type="entry name" value="PHAX_RNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038092">
        <property type="entry name" value="PHAX_RNA-binding_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13135">
        <property type="entry name" value="CYTOSOLIC RESINIFERATOXIN BINDING PROTEIN RBP-26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13135:SF0">
        <property type="entry name" value="PHOSPHORYLATED ADAPTER RNA EXPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10258">
        <property type="entry name" value="PHAX_RNA-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphorylated adapter RNA export protein RNA-binding" evidence="13">
        <location>
            <begin position="254"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="173"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="383"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002138568.2"/>
        </source>
    </evidence>
    <sequence length="462" mass="52010" checksum="7A109CB1B398FC90" modified="2020-08-12" version="1">MMELQLNDVDLEEGELSESDDDGIYTPLQRPAPTPAPAPGTSAESVASPHTTCHQTQTAALDDDESQTNSDSSSGESSEEGCIKKLRTDDPDSGHKKRKKRIKRTVMRRVAPTDTEMLPNRTRFKKYNVWTAALQEDALSENMRGCDVTRSSRDRNVENYDFSLRYRLNGENRLKRRLSNSSEDEAASHPTHKRGRPSSRPTEEEFSQRGSVKSRLGHRTRRGASSTSGSSDNSCEPRHILDLNDIAGRDPSDLATEMASKLYEEKDELLIRVVAVLGMDLPLELYKETQRIEADGGMMIKNGRRRRTPGGVFLFLLKHHDNITPEQQKSIFDEDRQNMNKSRKQIETLMRNRKVEELKKCLSKQSTELASLCQRKEHYMQADELSAESQPGNVSNPPPSPVGHEQESPEYKTHEININHVDNAELPSTSKAAALASSVGLKELVSYEDDFLDVNCGEMDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6X4</accession>
    <name>A0A6I8V6X4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein prom isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">prom</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_004444553.3">
        <property type="nucleotide sequence ID" value="XM_004444496.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_004444554.3">
        <property type="nucleotide sequence ID" value="XM_004444497.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6X4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0263808">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V6X4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11238">
        <property type="entry name" value="PROMININ ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11238:SF9">
        <property type="entry name" value="PROMININ, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="508"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="827"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="862"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1066"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1165"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1191"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="870"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="906"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="943"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="958"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1009"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1028"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1075"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1196"/>
            <end position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_004444553.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_004444554.3"/>
        </source>
    </evidence>
    <sequence length="1240" mass="138295" checksum="C2B6D77812C082C1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8I0</accession>
    <name>A0A6I8V8I0_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 19" type="primary">Pvr</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18 19">MV-25-SWS-2005</strain>
            <tissue evidence="18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_015036032.2">
        <property type="nucleotide sequence ID" value="XM_015180546.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036034.2">
        <property type="nucleotide sequence ID" value="XM_015180548.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V8I0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000373">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000384">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF600">
        <property type="entry name" value="PDGF- AND VEGF-RECEPTOR RELATED, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 18 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634487" description="receptor protein-tyrosine kinase" evidence="14">
        <location>
            <begin position="25"/>
            <end position="1649"/>
        </location>
    </feature>
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        <location>
            <begin position="916"/>
            <end position="937"/>
        </location>
    </feature>
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        <location>
            <begin position="372"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="489"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="593"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="695"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="816"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="991"/>
            <end position="1391"/>
        </location>
    </feature>
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        <location>
            <begin position="284"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1145"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1507"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1581"/>
            <end position="1649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1145"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1518"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1616"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="1025"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015036032.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015036034.2"/>
        </source>
    </evidence>
    <sequence length="1649" mass="185089" checksum="445EFCF3A810CA09" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAC8</accession>
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        <submittedName>
            <fullName evidence="6">Peroxisomal membrane protein 11C isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="primary">Pex11c</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
    <dbReference type="Bgee" id="FBgn0072599">
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    </dbReference>
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        <property type="term" value="C:peroxisomal membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PEX11C_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20990">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20990:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015037096.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="26262" checksum="72BEE3F2771849B7" modified="2020-08-12" version="1">MLNTIAGKQLTRCQIKKIIRCERKVMKALCYSAKLVAGYHAKRNPDLAKRYATASSKISGARATLRLIDDIPMIQYALEYGLGENEPDRVMAVLGVTANIVDLLYYPIEKVCWLAEHKIVDVKNADRWDNVNSIFWVLSVYLNLMRTMRNFSLNQEKLSCNPKLSDIDATVLAKHRLELVSIARISLDFVHAVSTLPGGYLWGGKLTTFQVGAIGTLSAGLGIYQIFAKRRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBJ6</accession>
    <name>A0A6I8VBJ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4802334</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359272.3">
        <property type="nucleotide sequence ID" value="XM_001359235.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037756.2">
        <property type="nucleotide sequence ID" value="XM_015182270.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBJ6"/>
    <dbReference type="KEGG" id="dpo:4802334"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359272.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015037756.2"/>
        </source>
    </evidence>
    <sequence length="328" mass="37625" checksum="0228F073ACA396A4" modified="2020-08-12" version="1">METTAMVPRAPARMMTPEELVLQSLTKQIFKSRLFRGYYLGRYFVEQIGDYRDVIALQRSERELEKLLIRDAGQLQMFYQRCVPNMWVGISYGSLGNYKHIPHPAQLETCIWAELNDLALGEYWFSIKTLRFRDQEVLLKLKTVRAVEPEPMATPRRSVSINRTTASNTITAAGESSTTEIQVTDQPPPLGLADFLEVLRQWGEGIKQATYDFMANDVNKHNIMGTIRFLGLLLFSIGGGAIAALRFLGIFAVRFLFELSRLSHTLTPILLQILQFLNNIVGGVFILFTMIWKDIITRGQPPTPKQPLEASTRFKSITYDRSDRRRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDE5</accession>
    <name>A0A6I8VDE5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Regucalcin</fullName>
            <ecNumber evidence="7">3.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Gluconolactonase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">smp-30</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucono-1,5-lactone + H2O = D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="EC" id="3.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.17" evidence="7"/>
    <dbReference type="RefSeq" id="XP_015038960.2">
        <property type="nucleotide sequence ID" value="XM_015183474.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDE5"/>
    <dbReference type="FunCoup" id="A0A6I8VDE5">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532961"/>
    <dbReference type="InParanoid" id="A0A6I8VDE5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000027">
        <property type="entry name" value="Regucalcin homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF66">
        <property type="entry name" value="MIP34848P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="16">
        <location>
            <begin position="50"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00016808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015038960.2"/>
        </source>
    </evidence>
    <sequence length="338" mass="37492" checksum="C75C19E600DD3167" modified="2020-08-12" version="1">MSIKTFLINRPRFSSKALAERKVFNGSPTHNQISKMSYKVEALPDSYAGLGEGPHWDVDRQSLYYVDLEVGFIHRYDFRQNKVYKAQIEGETFASFILPIENRPQEFAVGCNRRCVVVQWDGVATVAKVLRVLFEVQPGLEDNRINDAKTDPRGRFVGGTMCCSGDIFTQWKGELYNWQAGGQVTKIRGEVGISNGLAWDVKAKKFYFIDTNTHEVVAYDYNVDTGAVANPKVVFDLRKIRPNTPLYPDGMTIDTEGNIYVATFNGGSVFKVNPSSGKILLEIKIPTTQITSVAFGGPQLDILFVTTANKFDQPVPAGTTYKVTGLSAKGLPGVPLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VG23</accession>
    <name>A0A6I8VG23_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phenoloxidase-activating factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Prophenoloxidase-activating factor II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4805060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015040409.2">
        <property type="nucleotide sequence ID" value="XM_015184923.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VG23"/>
    <dbReference type="FunCoup" id="A0A6I8VG23">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805060"/>
    <dbReference type="InParanoid" id="A0A6I8VG23"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000038">
        <property type="entry name" value="Serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041515">
        <property type="entry name" value="PPAF-2-like_Clip"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF129">
        <property type="entry name" value="LP15124P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18322">
        <property type="entry name" value="CLIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026213253" description="Phenoloxidase-activating factor 2" evidence="6">
        <location>
            <begin position="34"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="201"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00076468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015040409.2"/>
        </source>
    </evidence>
    <sequence length="465" mass="51382" checksum="32E5C875ABCF5692" modified="2020-08-12" version="1">MSGATADTATGATSRCRLLWMALAIAALVMVNAQDMPSPMCRDDELCTTVTHCTETDDSGRKGIGPRISRFCGTGRVCCERAQLESWNASLVDRVGRQQRTNRRPAGSSIRDKNGNVLEPEANESCGMNMECVPRKLCRDNIIIDDGRSIINPRIGVGQCSRSLHRCCEVNQKVDASDSPYVAKLNGFKYNNCGWSNPQGLIPDKDKYNYTEDVSIFGQFPWMVAIFTGRQQYLCGGTLIHPQLVITSSHNIVNETVDTLMARAGDWDLNSLDEPYEHQGRRIKQIILHPEFDPEALFNDIAILVLDEPVQLAPHIQPLCLPPPESPQVIDDLLSATCFATGWGAKDPNSDKLERTLKRIDLPIVDHDECQAMLRNTRLESRFRLRPSFLCAGGIEGKDTCKGDGGSPLFCTLPGQTDRYQLAGIVSWGIECAEADIPSVYANVPYLRAWIDEKIKGLGLQLAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VL35</accession>
    <name>A0A6I8VL35_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Breast cancer anti-estrogen resistance protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CRK-associated substrate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">p130cas</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">p130CAS</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CAS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043592.2">
        <property type="nucleotide sequence ID" value="XM_015188106.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VL35"/>
    <dbReference type="FunCoup" id="A0A6I8VL35">
        <property type="interactions" value="436"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VL35"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11564">
        <property type="entry name" value="FAT-like_CAS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11549">
        <property type="entry name" value="Serine_rich_CAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11844">
        <property type="entry name" value="SH3_CAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.830:FF:000001">
        <property type="entry name" value="BCAR1 scaffold protein, Cas family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000001">
        <property type="entry name" value="Breast cancer anti-estrogen resistance 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000009">
        <property type="entry name" value="Breast cancer anti-estrogen resistance 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.830">
        <property type="entry name" value="Serine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021901">
        <property type="entry name" value="CAS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037362">
        <property type="entry name" value="CAS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014928">
        <property type="entry name" value="Serine_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038319">
        <property type="entry name" value="Serine_rich_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654">
        <property type="entry name" value="CAS SCAFFOLDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654:SF18">
        <property type="entry name" value="IP17195P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12026">
        <property type="entry name" value="CAS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08824">
        <property type="entry name" value="Serine_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="11" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="127"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="240"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="326"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="425"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="655"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="724"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="730"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015043592.2"/>
        </source>
    </evidence>
    <sequence length="891" mass="95817" checksum="2FE2814F31688C0C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPQ2</accession>
    <name>A0A6I8VPQ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4803304</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232734.1">
        <property type="nucleotide sequence ID" value="XM_033376843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPQ2"/>
    <dbReference type="KEGG" id="dpo:4803304"/>
    <dbReference type="InParanoid" id="A0A6I8VPQ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031959">
        <property type="entry name" value="DUF4779"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16009">
        <property type="entry name" value="DUF4779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026206061" evidence="2">
        <location>
            <begin position="17"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033232734.1"/>
        </source>
    </evidence>
    <sequence length="221" mass="25625" checksum="73F1A4943AEE861B" modified="2020-08-12" version="1">MWQLALALGLLQQLNAPPASPTATAIVVERNMFAYYKVPAAQLLLDTEETSSSSSGSSSDSKKFKSDLYFVLQCPPKLDTGIPKPKDMQTCSVVDQYKGMWKKPSPPQPKKHLHMVPFRIVMRPLRPPAMRRTYRRRKRHQNSDQEDDEEESTPLQHLGQPHIRQKLLKLKADHRKFKRGSSSQGYQHMYHRDESYNDHIFYDDLQADGQYHKYGKQIQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQD3</accession>
    <name>A0A6I8VQD3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Chymotrypsin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC117183393</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033233088.1">
        <property type="nucleotide sequence ID" value="XM_033377197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQD3"/>
    <dbReference type="KEGG" id="dpo:117183393"/>
    <dbReference type="InParanoid" id="A0A6I8VQD3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VQD3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000036">
        <property type="entry name" value="Trypsin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026203400" evidence="7">
        <location>
            <begin position="21"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033233088.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="16424" checksum="578AF648B13E7C32" modified="2020-08-12" version="1">MLMNFTRCALGLTVVVVAVALPTAAFPQGTAELLFTGWGSQSVAGTLPVQLQRVQQQHITSQVCSTLLAAYEDVELGACHVCGFRQANIGACHGDSGGPLVYEGTLVGILNFFVPCAQGVPDLFMNVMYYRDWIRQTMSGNGKCSQVHKQQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VR14</accession>
    <name>A0A6I8VR14_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6897316</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_033233169.1">
        <property type="nucleotide sequence ID" value="XM_033377278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VR14"/>
    <dbReference type="InParanoid" id="A0A6I8VR14"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VR14">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026088345" description="procollagen-proline 4-dioxygenase" evidence="13">
        <location>
            <begin position="21"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="370"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033233169.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="60897" checksum="B6CC84D825B9ED64" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VS62</accession>
    <name>A0A6I8VS62_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC117183536</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233519.1">
        <property type="nucleotide sequence ID" value="XM_033377628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VS62"/>
    <dbReference type="KEGG" id="dpo:117183536"/>
    <dbReference type="InParanoid" id="A0A6I8VS62"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VS62">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
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    </dbReference>
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        <property type="entry name" value="SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED PROTEIN"/>
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        <property type="entry name" value="GPROTEINBRPT"/>
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        <property type="entry name" value="WD40 repeat-like"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="142"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="300"/>
            <end position="338"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033233519.1"/>
        </source>
    </evidence>
    <sequence length="338" mass="37514" checksum="0B342A2C9D3C8B95" modified="2020-08-12" version="1">MDLNFPFGTPLVGHMDSVLDLSFSKVCDSGFYLASVGRDGQGIVRRGDTGEIFTSLLGHQRAVRGVYMSENATIVATGSDDCTARIWSVSSGNELAKFRHTQKVSSLALDTGSERMLTCSQERESNICLYDVNQSREVLCVYQKKLRGVRNVIFCRDDRSFLASSYDRTIELWDILSGQSSHKISLPHHAKSVELCADGKTVTIGYGESVVFFDADTFSIHGHHKIPFKVIGASLHPQKKSFVCASLEGKILKFNYDTGEQEDRFRAHVQMGHNISGIKYSPDGEMYVSSSTDGQITFWSQTSGNQNSDEDSIASYSSDSRDDFLDEGDGYSEQEDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VUL9</accession>
    <name>A0A6I8VUL9_DROPS</name>
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        <submittedName>
            <fullName evidence="7">NACHT domain- and WD repeat-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4804821</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234767.1">
        <property type="nucleotide sequence ID" value="XM_033378876.1"/>
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        <property type="interactions" value="66"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="NWD1/2-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
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    <dbReference type="PANTHER" id="PTHR19871:SF28">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871">
        <property type="entry name" value="BETA TRANSDUCIN-RELATED PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NWD1/2-like winged helix-turn-helix" evidence="5">
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            <begin position="565"/>
            <end position="669"/>
        </location>
    </feature>
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        <location>
            <begin position="940"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="984"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1026"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1109"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1254"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1296"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1376"/>
            <end position="1417"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1418"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1461"/>
            <end position="1502"/>
        </location>
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        <location>
            <begin position="721"/>
            <end position="745"/>
        </location>
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        <location>
            <begin position="1194"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1654"/>
            <end position="1684"/>
        </location>
    </feature>
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        <location>
            <begin position="1727"/>
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        </location>
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        <location>
            <begin position="1888"/>
            <end position="1929"/>
        </location>
    </feature>
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        <location>
            <begin position="1196"/>
            <end position="1208"/>
        </location>
    </feature>
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        <location>
            <begin position="1731"/>
            <end position="1760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1907"/>
            <end position="1922"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033234767.1"/>
        </source>
    </evidence>
    <sequence length="1929" mass="216831" checksum="B19C5FB545490623" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VV98</accession>
    <name>A0A6I8VV98_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein toll-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6898466</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033234868.1">
        <property type="nucleotide sequence ID" value="XM_033378977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VV98"/>
    <dbReference type="FunCoup" id="A0A6I8VV98">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898466"/>
    <dbReference type="InParanoid" id="A0A6I8VV98"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000727">
        <property type="entry name" value="Toll-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF530">
        <property type="entry name" value="MSTPROX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026156311" evidence="9">
        <location>
            <begin position="18"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="10">
        <location>
            <begin position="839"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033234868.1"/>
        </source>
    </evidence>
    <sequence length="999" mass="116160" checksum="E49E5ED3ACF1B9F9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWE9</accession>
    <name>A0A6I8VWE9_DROPS</name>
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        <submittedName>
            <fullName evidence="14 15">Nuclear hormone receptor FTZ-F1 beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">Hr39</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15">MV-25-SWS-2005</strain>
            <tissue evidence="14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033235411.1">
        <property type="nucleotide sequence ID" value="XM_033379520.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235412.1">
        <property type="nucleotide sequence ID" value="XM_033379521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWE9"/>
    <dbReference type="KEGG" id="dpo:4816657"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07167">
        <property type="entry name" value="NR_DBD_Lrh-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06930">
        <property type="entry name" value="NR_LBD_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000037">
        <property type="entry name" value="Nuclear hormone receptor FTZ-F1 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016355">
        <property type="entry name" value="NR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086:SF25">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR FTZ-F1 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="8 14 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="11">
        <location>
            <begin position="407"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="12">
        <location>
            <begin position="636"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="121"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="214"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="360"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="519"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="612"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="295"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="612"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033235411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033235412.1"/>
        </source>
    </evidence>
    <sequence length="863" mass="93286" checksum="2DF8EBFA6BA02881" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W086</accession>
    <name>A0A6I8W086_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4817516</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_033380851.1"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033041">
        <property type="term" value="F:sweet taste receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF131">
        <property type="entry name" value="GUSTATORY AND ODORANT RECEPTOR 63A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033236742.1"/>
        </source>
    </evidence>
    <sequence length="386" mass="44272" checksum="59212E9326577D93" modified="2020-08-12" version="1">MLRRREGFAPKLCWLVLQVTLYGSWAFGLFPFTLDPQTRQLRRHRWLLVYGVAINLFLLCIVVLLSEYSEETAQSLEVFQRNHLLGVMNVIIGVLALIASCGIILISFWKSGQALCIFNELLGLEHRHFGCLDVEDSSKFNLYVIQKGMSVVGELMGLVVVNYGMPEYTMSYLYLALLCLTQFCVNLVVTQIYLAILYIYRSVWLINRQLLGVVSRLRVDPLSDSSRIPLLLSLYGRLLALHKQMETTFNGQITLILTSALAGNIVVIYFLIVYTVSLGQLSISLAIFPYSLIMNVWDYWLSISVCDLTERTGRHTSTILKLFSDLEHTDEDLERSINEFTWLCSHRRFRFGLYGLCSVNSETGFQMIVTSFMYLLCLVQFDFMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3E0</accession>
    <name>A0A6I8W3E0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6897795</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237823.1">
        <property type="nucleotide sequence ID" value="XM_033381932.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:6897795"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21113">
        <property type="entry name" value="AGAP001705-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21113:SF4">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026082280" description="Chitin-binding type-4 domain-containing protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-4" evidence="4">
        <location>
            <begin position="29"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033237823.1"/>
        </source>
    </evidence>
    <sequence length="365" mass="40916" checksum="7B367A6AD4668A8B" modified="2020-08-12" version="1">MPRLGQALPKLWALLLYAPLFFIRSCDGHGRLIEPPSRASAWRYGFQTPPDYNDHELYCGGFTRQWKRNGGKCGECGDAWDMPEPRPHENGGQWGQGVIVRSYLPASEMTIRVELTASHMGYFEFRLCDDPGAKQACLDKNILHILGGSPAQPSPADMDSRFYPRNGSRIYEIKARLPELTCNQCVLQWRYVAGNNWGMCNDGNGAVGCGPQEEFRSCSDIAITTDARPPMRPIRPLHPTPTRSTTKIPTTNEYDSEENAFNYSLLLALVITLGVVFCAIGIWYFYRRNFCIFKECSNSVAQKFRNNNKNNASLENAVHNRIFKPLESLGYGGKGVEVDNPPVPPPRTKRDGRMRELSANASSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3S1</accession>
    <name>A0A6I8W3S1_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Calponin homology domain-containing protein DDB_G0272472 isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC6897563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
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    </reference>
    <reference evidence="4" key="2">
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237958.1">
        <property type="nucleotide sequence ID" value="XM_033382067.1"/>
    </dbReference>
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        <property type="interactions" value="55"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:centrosome cycle"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035148">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031887">
        <property type="entry name" value="SDCCAG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34343">
        <property type="entry name" value="SEROLOGICALLY DEFINED COLON CANCER ANTIGEN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SEROLOGICALLY DEFINED COLON CANCER ANTIGEN 8"/>
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    </dbReference>
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        <property type="entry name" value="CCCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="43"/>
        </location>
    </feature>
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        </location>
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            <end position="324"/>
        </location>
    </feature>
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            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="924"/>
        </location>
    </feature>
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            <end position="1078"/>
        </location>
    </feature>
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            <end position="1120"/>
        </location>
    </feature>
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            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
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        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
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            <end position="728"/>
        </location>
    </feature>
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            <begin position="731"/>
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        </location>
    </feature>
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        </location>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1008"/>
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        </location>
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            <begin position="1042"/>
            <end position="1055"/>
        </location>
    </feature>
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        <location>
            <begin position="1059"/>
            <end position="1069"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033237958.1"/>
        </source>
    </evidence>
    <sequence length="1208" mass="135829" checksum="AC9845286CAE7F48" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3S9</accession>
    <name>A0A6I8W3S9_DROPS</name>
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        <recommendedName>
            <fullName evidence="19">Protein sevenless</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="26 27 28 29" type="primary">sev</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26 27 28">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033237999.1">
        <property type="nucleotide sequence ID" value="XM_033382108.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238000.1">
        <property type="nucleotide sequence ID" value="XM_033382109.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238001.1">
        <property type="nucleotide sequence ID" value="XM_033382110.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238002.1">
        <property type="nucleotide sequence ID" value="XM_033382111.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W3S9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Protein sevenless"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="TolB, C-terminal domain"/>
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    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="17" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="2101"/>
            <end position="2122"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="427"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="804"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="1282"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="24">
        <location>
            <begin position="1783"/>
            <end position="1879"/>
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    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="2185"/>
            <end position="2457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="32"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="51"/>
            <end position="71"/>
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    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="509"/>
            <end position="536"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="2488"/>
            <end position="2534"/>
        </location>
    </feature>
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        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="526"/>
            <end position="535"/>
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    </feature>
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        <location>
            <begin position="2512"/>
            <end position="2526"/>
        </location>
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        <location>
            <position position="2218"/>
        </location>
        <ligand>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069330"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_033237999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_033238000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_033238001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_033238002.1"/>
        </source>
    </evidence>
    <sequence length="2534" mass="285149" checksum="37481A20B7959DCF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W464</accession>
    <name>A0A6I8W464_DROPS</name>
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        <submittedName>
            <fullName evidence="10 11 12">Death-associated inhibitor of apoptosis 1 isoform X1</fullName>
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        <name evidence="10 11 12" type="primary">Diap1</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11 12">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <text evidence="1">Belongs to the IAP family.</text>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of protein ubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Baculoviral IAP repeat containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
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    </dbReference>
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        <property type="entry name" value="Death-associated inhibitor of apoptosis 1"/>
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    </dbReference>
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        <property type="entry name" value="E3 ubiquitin-protein ligase XIAP"/>
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    </dbReference>
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        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BIR_rpt"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044:SF174">
        <property type="entry name" value="DEATH-ASSOCIATED INHIBITOR OF APOPTOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044">
        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BIR"/>
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    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
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            <end position="427"/>
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    <sequence length="439" mass="48202" checksum="BCF465BF0E1DED12" modified="2020-08-12" version="1">MANVLTNPRYMYGPTAALELTAVLDQVDNNTNATQLFKNNIYNIMNEYNREEARLKTFANWPLAWLDKHQLARTGMFYTNDNDKVKCYFCEVEIGRWDLDDQPVPEHLRWSPNCPLLRRRTTNNVPLNSEALDRILPPISYDICGANDSTVELRESAYAEGRIPMSHIAQSIATNTAVSPLMSSIATSTAATQANGDVHPESCRQVASGNYFPEHPEYAVESTRLRSFEEWPRNLKQKPMQLAEAGFFYTGVGDRVRCFSCGGGLKDWDDNDEPWEQHALWLSQCRFVKLMKGQRYIDTVAAKPVPVAEKEESSTSADETPAAASSTTTTPAATATVETPFGDVGPSAATCIYEKIVSSASTTTATGAIPGTAGGPPAAPNGSPVIPEEKLCKICYAAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFSDVMRVYFS</sequence>
</entry>
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    <accession>A0A6I8W490</accession>
    <name>A0A6I8W490_DROPS</name>
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        <submittedName>
            <fullName evidence="12">Uncharacterized protein isoform X1</fullName>
        </submittedName>
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        <name evidence="12" type="primary">LOC117184412</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="12">MV-25-SWS-2005</strain>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MCP_dom_sf"/>
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        <property type="entry name" value="MCP_transmembrane"/>
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    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
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        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
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        <property type="entry name" value="Mito_carr"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033237509.1"/>
        </source>
    </evidence>
    <sequence length="878" mass="95619" checksum="832F045FF632DFB8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W680</accession>
    <name>A0A6I8W680_DROPS</name>
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        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein rudhira isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="4 5" type="primary">rudhira</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="4 5">MV-25-SWS-2005</strain>
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        <property type="entry name" value="BCAS3 MICROTUBULE ASSOCIATED CELL MIGRATION FACTOR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BREAST CARCINOMA AMPLIFIED SEQUENCE 3"/>
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            <end position="1850"/>
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            <begin position="1851"/>
            <end position="1861"/>
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            <begin position="1874"/>
            <end position="1883"/>
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            <begin position="2016"/>
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            <begin position="2080"/>
            <end position="2114"/>
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            <begin position="2135"/>
            <end position="2144"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <sequence length="2144" mass="229449" checksum="4529DD8110909DD0" modified="2020-08-12" version="1"> </sequence>
</entry>
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        <name evidence="6" type="primary">LOC4812274</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
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        <name evidence="3" type="primary">LOC117184918</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <dbReference type="RefSeq" id="XP_033239999.1"/>
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    <sequence length="149" mass="17732" checksum="6381DDA99B7D513A" modified="2020-08-12" version="1">MPPFIRDDKPLAWDKEDQDQNQDQDQNQDQNQNQDHDHDQDLHQNEEPIVENDELSDSELYRTLFQVRTTVEQNRADAERAGLNMESFHQRLEAYSDLLIEFTNAVNRKLALNRQGHTAETAMDHSAVSQQIHRLFREISEYFDNYMRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBR8</accession>
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    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="14 15 16">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
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        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        </reaction>
    </comment>
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        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_033384956.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:ubiquitin ligase complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16483">
        <property type="entry name" value="RING-H2_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19673">
        <property type="entry name" value="UBR-box_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3_ligase_UBR-like_C"/>
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    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF39">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="12">
        <location>
            <begin position="228"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="228"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="93"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1119"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1360"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1466"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1898"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1956"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2062"/>
            <end position="2081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1124"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1373"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1394"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1956"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="2062"/>
            <end position="2075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033240845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033240846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033240847.1"/>
        </source>
    </evidence>
    <sequence length="2336" mass="257713" checksum="62549DDDF4E63EF0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDQ3</accession>
    <name>A0A6I8WDQ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC117185414</name>
        <name evidence="5" type="synonym">LOC117183790</name>
        <name evidence="6" type="synonym">LOC117184112</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235116.1">
        <property type="nucleotide sequence ID" value="XM_033379225.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236208.1">
        <property type="nucleotide sequence ID" value="XM_033380317.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033385670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDQ3"/>
    <dbReference type="KEGG" id="dpo:117183790"/>
    <dbReference type="KEGG" id="dpo:117184112"/>
    <dbReference type="KEGG" id="dpo:117185414"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
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    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033236208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033241561.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="40762" checksum="0217314016E9EFC0" modified="2020-08-12" version="1">MAEETMANIDKEKIVEWLLEKEIIFPASATLRQLRKLAISGGMDEVSESLVNKSDIESEEKVSENEQIDDMKEEELLDAAIRVAEKKKKLAALMVMDNHVTDDIRMVKQLIAPFSASENDEASEWVLNFERICGGVNESSNFKLRCVRMLMKAGTEADLFMRVDKSKTYDEFKGNFLKTFGRSNSTADVVLLLKETFFNPEKNSVMGYILRMEEIAMRADIEEKLTVQFIIDGFRDRSSSIALLYSASTIGKLKELARQYEALRKSTQTNFRRTGMTASGNDRSQARCYNCSAHGHYASSCPAPKREKGSCFQCGSMQHQVKDCQQKPMSAQRSVGTASNPPMDDREENNIFVPIVNQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WE22</accession>
    <name>A0A6I8WE22_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Uracil phosphoribosyltransferase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">kri</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241477.1">
        <property type="nucleotide sequence ID" value="XM_033385586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WE22"/>
    <dbReference type="FunCoup" id="A0A6I8WE22">
        <property type="interactions" value="882"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812128"/>
    <dbReference type="InParanoid" id="A0A6I8WE22"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078954">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WE22">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000026">
        <property type="entry name" value="Uracil phosphoribosyltransferase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="66"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033241477.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="28551" checksum="664C19BA3571C115" modified="2020-08-12" version="1">MCTGSPSSNDSQSEEANATTEQQQQPEQEATEQPIRTPTHAIVAAAKAEEILAEYGSNLKLLECNSQVSELLTIIRDKNTTRSDFKFYADRLIRLVIEESLNQLPYTDCDVETPTGAIYEGLKYRSGNCGVSIIRSGEAMEQGLRDCCRSIRIGKILVESDANTHEARVVYARFPDDIGSRQVLLMYPIMSTGNTVLQAVNVLREHGVPEKCIILSNLFCTPVAARTVVNAFPQLKILTSELHPVAPNHFGQKYFDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEH6</accession>
    <name>A0A6I8WEH6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6896993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241798.1">
        <property type="nucleotide sequence ID" value="XM_033385907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEH6"/>
    <dbReference type="KEGG" id="dpo:6896993"/>
    <dbReference type="InParanoid" id="A0A6I8WEH6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033241798.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="20621" checksum="C7B60DC1F7204EF4" modified="2020-08-12" version="1">MDNTSQVDNSEPSFQDAVVFKFTNFVCESYNQSWFVFHNYRLKAVSRNKVLLNMNATILHPANNISVHGRIFKKANGYKPWLFDIYFDVCQYLRKRNQPFINIVYGLFKDFSNLNHSCPYVGPQIIKDFYLRPELLRLPFPTGDYLVAIQWYFDKKPLADTNVSFTFVEDLMNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEX7</accession>
    <name>A0A6I8WEX7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Membrane-bound transcription factor site-1 protease</fullName>
            <ecNumber evidence="24">3.4.21.112</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Endopeptidase S1P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="35" type="primary">S1P</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="35">MV-25-SWS-2005</strain>
            <tissue evidence="35">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Processes precursors containing basic and hydrophobic/aliphatic residues at P4 and P2, respectively, with a relatively relaxed acceptance of amino acids at P1 and P3.</text>
            <dbReference type="EC" id="3.4.21.112"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.112" evidence="24"/>
    <dbReference type="RefSeq" id="XP_033241224.1">
        <property type="nucleotide sequence ID" value="XM_033385333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEX7"/>
    <dbReference type="FunCoup" id="A0A6I8WEX7">
        <property type="interactions" value="893"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117185167"/>
    <dbReference type="InParanoid" id="A0A6I8WEX7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07479">
        <property type="entry name" value="Peptidases_S8_SKI-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000008">
        <property type="entry name" value="Membrane-bound transcription factor site-1 protease preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055143">
        <property type="entry name" value="MBTP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057060">
        <property type="entry name" value="MBTPS1_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057032">
        <property type="entry name" value="MBTPS1_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034185">
        <property type="entry name" value="Site-1_peptidase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF7">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23001">
        <property type="entry name" value="MBTP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23094">
        <property type="entry name" value="MBTPS1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23090">
        <property type="entry name" value="MBTPS1_4th"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 27" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 27 35" id="KW-0645">Protease</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 27" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9 29" id="KW-0732">Signal</keyword>
    <keyword evidence="22" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="8 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="19" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="29">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026201855" description="Membrane-bound transcription factor site-1 protease" evidence="29">
        <location>
            <begin position="28"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="1001"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane-bound transcription factor site-1 protease-like N-terminal" evidence="31">
        <location>
            <begin position="37"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="30">
        <location>
            <begin position="172"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="MBTPS1 third" evidence="33">
        <location>
            <begin position="449"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="MBTPS1 fourth" evidence="32">
        <location>
            <begin position="577"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="MBTPS1 fourth" evidence="32">
        <location>
            <begin position="833"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00066596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00067283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF23001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF23090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF23094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_033241224.1"/>
        </source>
    </evidence>
    <sequence length="1025" mass="115306" checksum="056E943E5A9BD2B6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WF38</accession>
    <name>A0A6I8WF38_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117185212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241529.1">
        <property type="nucleotide sequence ID" value="XM_033385638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WF38"/>
    <dbReference type="KEGG" id="dpo:117185212"/>
    <dbReference type="InParanoid" id="A0A6I8WF38"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033241529.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="15129" checksum="55B5408723CFA930" modified="2020-08-12" version="1">MNGQNRKREWQAGRGEDLTPDDRIELIAELMAKLDGVNFVADFLHIRRLKLLITDCLPDRKEQLLRILIGYVNRPPSPATTSYAKIVSLLSNDFGSLMVDCIRALRAVQEKALIDGKWDNARGLEYFLAELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8F983</accession>
    <name>A0ABD8F983_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein ST7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4813442</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ST7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353227.2">
        <property type="nucleotide sequence ID" value="XM_001353191.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8F983"/>
    <dbReference type="GeneID" id="4813442"/>
    <dbReference type="KEGG" id="dpo:4813442"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11557">
        <property type="entry name" value="ST7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007311">
        <property type="entry name" value="ST7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12745:SF6">
        <property type="entry name" value="PROTEIN ST7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12745">
        <property type="entry name" value="SUPPRESSION OF TUMORIGENICITY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04184">
        <property type="entry name" value="ST7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353227.2"/>
        </source>
    </evidence>
    <sequence length="541" mass="60475" checksum="1AEE4967D937590D" modified="2025-06-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>B5DNS4</accession>
    <accession>A0A6I8V053</accession>
    <name>B5DNS4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Endonuclease G, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6901070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134549.2">
        <property type="nucleotide sequence ID" value="XM_002134513.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DNS4"/>
    <dbReference type="SMR" id="B5DNS4"/>
    <dbReference type="FunCoup" id="B5DNS4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DNS4"/>
    <dbReference type="GeneID" id="6901070"/>
    <dbReference type="KEGG" id="dpo:6901070"/>
    <dbReference type="eggNOG" id="KOG3721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_674887_0_0_1"/>
    <dbReference type="InParanoid" id="B5DNS4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243767">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DNS4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF5">
        <property type="entry name" value="ENDONUCLEASE G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="5">
        <location>
            <begin position="121"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="4">
        <location>
            <begin position="122"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002134549.2"/>
        </source>
    </evidence>
    <sequence length="331" mass="37757" checksum="7E1FF9D18A7BBDBB" modified="2016-01-20" version="2">MPQPQDIALYASVGVAAFVCGAFLQQETSINNLFDVIKRDPYVYHNRRKLYPVLSTFSTNHEARLWSRPVGLKDKICDRVKPLLKFLLPKPKEEMGSEEPPLANVLDLLKYGLPSAEHLVVHKDYIMSRGMETKSATWICEHFRAGSSPDEPEYLRYTDVFLLTGACEEIGRVFRRPIWQELERYVTLKTQECGSLYTYTGPIFTPKNHGTGKSWLEYRECGNASMPVPVPSHYFKVLIIEESQVPGGDVQPVNIEAYVIKNGPSDMSGCLCEHRVRIKDIEEQTGLRFNKEQQGPKVIKNKDNIEIEFTCEAMQMMSSSQLMTTDQCCFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>B5DP63</accession>
    <accession>A0A6I8V0A0</accession>
    <name>B5DP63_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein KIAA1143 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134688.1">
        <property type="nucleotide sequence ID" value="XM_002134652.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DP63"/>
    <dbReference type="FunCoup" id="B5DP63">
        <property type="interactions" value="1689"/>
    </dbReference>
    <dbReference type="GeneID" id="6900992"/>
    <dbReference type="KEGG" id="dpo:6900992"/>
    <dbReference type="eggNOG" id="ENOG502S9DQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111288_0_0_1"/>
    <dbReference type="InParanoid" id="B5DP63"/>
    <dbReference type="OMA" id="DPQDQED"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245750">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DP63">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027911">
        <property type="entry name" value="DUF4604"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040219">
        <property type="entry name" value="KIAA1143-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31195">
        <property type="entry name" value="GEO02494P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31195:SF2">
        <property type="entry name" value="GEO02494P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15377">
        <property type="entry name" value="DUF4604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134688.1"/>
        </source>
    </evidence>
    <sequence length="156" mass="17333" checksum="63E7D2EBB143A5BC" modified="2008-10-14" version="1">MSKRNNITYVKPQEPSFLAKLKAEIGYKEGPSVDTKRQRLEDEDPEGSDSGEERPEREDEQPQVVVLQAGDLSAAEALEEKRLAAKELAEKRADLSQPIVFKQRVKQPNVEEEADKTKPKKSKKSDKGSKKSKAAAVSSSKLSFNEDEEDSEGGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>B5DR49</accession>
    <accession>A0A6I8V1D3</accession>
    <name>B5DR49_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor eIF1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein translation factor SUI1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eIF1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135439.1">
        <property type="nucleotide sequence ID" value="XM_002135403.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DR49"/>
    <dbReference type="SMR" id="B5DR49"/>
    <dbReference type="FunCoup" id="B5DR49">
        <property type="interactions" value="1091"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DR49"/>
    <dbReference type="GeneID" id="6900076"/>
    <dbReference type="KEGG" id="dpo:6900076"/>
    <dbReference type="eggNOG" id="KOG1770">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_3_0_1"/>
    <dbReference type="InParanoid" id="B5DR49"/>
    <dbReference type="OMA" id="VENHIHI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249688">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DR49">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000010">
        <property type="entry name" value="Protein translation factor SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="7">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00071306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002135439.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="12450" checksum="0E82648472CB6D1B" modified="2008-10-14" version="1">MSIQNLNTRDPFADAIKGNDDDIQDGLVHIRIQQRNGRKTLTTVQGLSAEYDLKKIVRSCKKEFACNGTVIEHPEYGEVLQLQGDQRENICQWLTKVGLAKPDQLKVHGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1991-08-01" modified="2026-01-28" version="160" xmlns="https://uniprot.org/uniprot">
    <accession>P22811</accession>
    <accession>Q299Q9</accession>
    <accession>Q56RA2</accession>
    <name>XDH_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Xanthine dehydrogenase</fullName>
            <shortName>XD</shortName>
            <ecNumber>1.17.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Protein rosy locus</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ry</name>
        <name type="synonym">XDH</name>
        <name type="ORF">GA20500</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1989" name="Mol. Biol. Evol." volume="6" first="33" last="52">
            <title>Nucleotide sequence of the Xdh region in Drosophila pseudoobscura and an analysis of the evolution of synonymous codons.</title>
            <authorList>
                <person name="Riley M.A."/>
            </authorList>
            <dbReference type="PubMed" id="2493563"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Genetics" volume="169" first="1495" last="1507">
            <title>Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda.</title>
            <authorList>
                <person name="Bartolome C."/>
                <person name="Maside X."/>
                <person name="Yi S."/>
                <person name="Grant A.L."/>
                <person name="Charlesworth B."/>
            </authorList>
            <dbReference type="PubMed" id="15545653"/>
            <dbReference type="DOI" id="10.1534/genetics.104.033068"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 298-1062</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>xanthine + NAD(+) + H2O = urate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>hypoxanthine + NAD(+) + H2O = xanthine + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24670"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
        <text evidence="1">Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="2">Binds 2 [2Fe-2S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the xanthine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.4"/>
    <dbReference type="EMBL" id="M33977">
        <property type="protein sequence ID" value="AAA29022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL27642.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY754605">
        <property type="protein sequence ID" value="AAX13180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A31946">
        <property type="entry name" value="A31946"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P22811"/>
    <dbReference type="SMR" id="P22811"/>
    <dbReference type="FunCoup" id="P22811">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="46245.P22811"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0283786">
        <property type="protein sequence ID" value="FBpp0282224"/>
        <property type="gene ID" value="FBgn0012736"/>
    </dbReference>
    <dbReference type="GeneID" id="4801405"/>
    <dbReference type="KEGG" id="dpo:4801405"/>
    <dbReference type="CTD" id="41605"/>
    <dbReference type="eggNOG" id="KOG0430">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_1_2_1"/>
    <dbReference type="InParanoid" id="P22811"/>
    <dbReference type="OMA" id="PHPTQER"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0012736">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004854">
        <property type="term" value="F:xanthine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009115">
        <property type="term" value="P:xanthine catabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.120:FF:000001">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000015">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000003">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.50:FF:000001">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Xanthine dehydrogenase/oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000004">
        <property type="entry name" value="Xanthine dehydrogenase/oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022407">
        <property type="entry name" value="OxRdtase_Mopterin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014307">
        <property type="entry name" value="Xanthine_DH_ssu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02963">
        <property type="entry name" value="xanthine_xdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45444">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45444:SF3">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000127">
        <property type="entry name" value="Xanthine_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
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        <property type="entry name" value="2FE2S_FER_2"/>
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    <dbReference type="PROSITE" id="PS00559">
        <property type="entry name" value="MOLYBDOPTERIN_EUK"/>
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            <end position="357"/>
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    <feature type="binding site" evidence="1">
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    <feature type="binding site" evidence="1">
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    <feature type="binding site" evidence="1">
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            <position position="780"/>
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        <ligandPart>
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            <position position="811"/>
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            <name>Mo</name>
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    <feature type="binding site" evidence="1">
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            <position position="893"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
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            <position position="925"/>
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        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
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    <feature type="binding site" evidence="1">
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            <position position="927"/>
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        <ligand>
            <name>substrate</name>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="1092"/>
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        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
        </ligandPart>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA29022." evidence="5" ref="1">
        <original>V</original>
        <variation>M</variation>
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA29022." evidence="5" ref="1">
        <original>T</original>
        <variation>A</variation>
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA29022 and 3; AAX13180." evidence="5" ref="1 3">
        <original>V</original>
        <variation>I</variation>
        <location>
            <position position="428"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA29022 and 3; AAX13180." evidence="5" ref="1 3">
        <original>G</original>
        <variation>E</variation>
        <location>
            <position position="461"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA29022." evidence="5" ref="1">
        <original>L</original>
        <variation>V</variation>
        <location>
            <position position="475"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AAX13180." evidence="5" ref="3">
        <original>E</original>
        <variation>D</variation>
        <location>
            <position position="734"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA29022." evidence="5" ref="1">
        <original>S</original>
        <variation>N</variation>
        <location>
            <position position="747"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA29022." evidence="5" ref="1">
        <original>A</original>
        <variation>G</variation>
        <location>
            <position position="762"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AAX13180." evidence="5" ref="3">
        <original>A</original>
        <variation>V</variation>
        <location>
            <position position="986"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA29022." evidence="5" ref="1">
        <location>
            <position position="1245"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA29022." evidence="5" ref="1">
        <original>C</original>
        <variation>F</variation>
        <location>
            <position position="1320"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P22985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="1343" mass="147349" checksum="A58C142336E14BC9" modified="2006-07-11" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CQ0</accession>
    <accession>A0A6I8UAB9</accession>
    <name>Q29CQ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transcription initiation factor TFIID subunit 3 isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein, isoform C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Dpse\GA15182</name>
        <name evidence="13" type="synonym">Taf3</name>
        <name evidence="11" type="ORF">Dpse_GA15182</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
                <person name="Castrezana S."/>
                <person name="Celniker S.E."/>
                <person name="Chang J.L."/>
                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
                <person name="Clifton S.W."/>
                <person name="Comeron J.M."/>
                <person name="Costello J.C."/>
                <person name="Coyne J.A."/>
                <person name="Daub J."/>
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                <person name="Godfrey J."/>
                <person name="Good R."/>
                <person name="Gotea V."/>
                <person name="Gravely B."/>
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                <person name="Griffiths-Jones S."/>
                <person name="Gross S."/>
                <person name="Guigo R."/>
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                <person name="Haerty W."/>
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                <person name="Halpern A.L."/>
                <person name="Halter G.M."/>
                <person name="Han M.V."/>
                <person name="Heger A."/>
                <person name="Hillier L."/>
                <person name="Hinrichs A.S."/>
                <person name="Holmes I."/>
                <person name="Hoskins R.A."/>
                <person name="Hubisz M.J."/>
                <person name="Hultmark D."/>
                <person name="Huntley M.A."/>
                <person name="Jaffe D.B."/>
                <person name="Jagadeeshan S."/>
                <person name="Jeck W.R."/>
                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
                <person name="Li R."/>
                <person name="Lin C.F."/>
                <person name="Lin M.F."/>
                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
                <person name="Machado C.A."/>
                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
                <person name="O'Grady P."/>
                <person name="Pachter L."/>
                <person name="Papaceit M."/>
                <person name="Parisi M.J."/>
                <person name="Parisi M."/>
                <person name="Parts L."/>
                <person name="Pedersen J.S."/>
                <person name="Pesole G."/>
                <person name="Phillippy A.M."/>
                <person name="Ponting C.P."/>
                <person name="Pop M."/>
                <person name="Porcelli D."/>
                <person name="Powell J.R."/>
                <person name="Prohaska S."/>
                <person name="Pruitt K."/>
                <person name="Puig M."/>
                <person name="Quesneville H."/>
                <person name="Ram K.R."/>
                <person name="Rand D."/>
                <person name="Rasmussen M.D."/>
                <person name="Reed L.K."/>
                <person name="Reenan R."/>
                <person name="Reily A."/>
                <person name="Remington K.A."/>
                <person name="Rieger T.T."/>
                <person name="Ritchie M.G."/>
                <person name="Robin C."/>
                <person name="Rogers Y.H."/>
                <person name="Rohde C."/>
                <person name="Rozas J."/>
                <person name="Rubenfield M.J."/>
                <person name="Ruiz A."/>
                <person name="Russo S."/>
                <person name="Salzberg S.L."/>
                <person name="Sanchez-Gracia A."/>
                <person name="Saranga D.J."/>
                <person name="Sato H."/>
                <person name="Schaeffer S.W."/>
                <person name="Schatz M.C."/>
                <person name="Schlenke T."/>
                <person name="Schwartz R."/>
                <person name="Segarra C."/>
                <person name="Singh R.S."/>
                <person name="Sirot L."/>
                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
                <person name="Smith T.F."/>
                <person name="Spieth J."/>
                <person name="Stage D.E."/>
                <person name="Stark A."/>
                <person name="Stephan W."/>
                <person name="Strausberg R.L."/>
                <person name="Strempel S."/>
                <person name="Sturgill D."/>
                <person name="Sutton G."/>
                <person name="Sutton G.G."/>
                <person name="Tao W."/>
                <person name="Teichmann S."/>
                <person name="Tobari Y.N."/>
                <person name="Tomimura Y."/>
                <person name="Tsolas J.M."/>
                <person name="Valente V.L."/>
                <person name="Venter E."/>
                <person name="Venter J.C."/>
                <person name="Vicario S."/>
                <person name="Vieira F.G."/>
                <person name="Vilella A.J."/>
                <person name="Villasante A."/>
                <person name="Walenz B."/>
                <person name="Wang J."/>
                <person name="Wasserman M."/>
                <person name="Watts T."/>
                <person name="Wilson D."/>
                <person name="Wilson R.K."/>
                <person name="Wing R.A."/>
                <person name="Wolfner M.F."/>
                <person name="Wong A."/>
                <person name="Wong G.K."/>
                <person name="Wu C.I."/>
                <person name="Wu G."/>
                <person name="Yamamoto D."/>
                <person name="Yang H.P."/>
                <person name="Yang S.P."/>
                <person name="Yorke J.A."/>
                <person name="Yoshida K."/>
                <person name="Zdobnov E."/>
                <person name="Zhang P."/>
                <person name="Zhang Y."/>
                <person name="Zimin A.V."/>
                <person name="Baldwin J."/>
                <person name="Abdouelleil A."/>
                <person name="Abdulkadir J."/>
                <person name="Abebe A."/>
                <person name="Abera B."/>
                <person name="Abreu J."/>
                <person name="Acer S.C."/>
                <person name="Aftuck L."/>
                <person name="Alexander A."/>
                <person name="An P."/>
                <person name="Anderson E."/>
                <person name="Anderson S."/>
                <person name="Arachi H."/>
                <person name="Azer M."/>
                <person name="Bachantsang P."/>
                <person name="Barry A."/>
                <person name="Bayul T."/>
                <person name="Berlin A."/>
                <person name="Bessette D."/>
                <person name="Bloom T."/>
                <person name="Blye J."/>
                <person name="Boguslavskiy L."/>
                <person name="Bonnet C."/>
                <person name="Boukhgalter B."/>
                <person name="Bourzgui I."/>
                <person name="Brown A."/>
                <person name="Cahill P."/>
                <person name="Channer S."/>
                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
                <person name="Costello M."/>
                <person name="D'Aco K."/>
                <person name="Daza R."/>
                <person name="De Haan G."/>
                <person name="DeGray S."/>
                <person name="DeMaso C."/>
                <person name="Dhargay N."/>
                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
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                <person name="Faro S."/>
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                <person name="Franke A."/>
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                <person name="Gearin C.R."/>
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                <person name="Graham J."/>
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                <person name="Gyaltsen K."/>
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                <person name="Hall J."/>
                <person name="Henson C."/>
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                <person name="Honan T."/>
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                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
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                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
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                <person name="Zimmer A."/>
                <person name="Zwirko Z."/>
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                <person name="Alvarez P."/>
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                <person name="Butler J."/>
                <person name="Chin C."/>
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        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
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                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
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            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="11">MV2-25</strain>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="13">MV-25-SWS-2005</strain>
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<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12 16">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12 16">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 16">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="5 12 16"/>
    <dbReference type="RefSeq" id="XP_001354447.1">
        <property type="nucleotide sequence ID" value="XM_001354411.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FS9"/>
    <dbReference type="SMR" id="Q29FS9"/>
    <dbReference type="FunCoup" id="Q29FS9">
        <property type="interactions" value="1164"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FS9"/>
    <dbReference type="GeneID" id="4814420"/>
    <dbReference type="KEGG" id="dpo:4814420"/>
    <dbReference type="eggNOG" id="KOG2653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024540_4_2_1"/>
    <dbReference type="InParanoid" id="Q29FS9"/>
    <dbReference type="OMA" id="CVTHVGP"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077653">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FS9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.320:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000007">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006765">
        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="7 12 16" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 12 16" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="17">
        <location>
            <begin position="180"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="187"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001354447.1"/>
        </source>
    </evidence>
    <sequence length="481" mass="52533" checksum="50373C5537048EE7" modified="2006-04-04" version="1">MSGKADIALIGLAVMGQNLILNMDEKGFVVCAYNRTVAKVKEFLANEAKGTNVIGAESLKDMVEKLKTPRKIMLLVKAGSAVDDFIEQLVPLLSRGDVIIDGGNSEYQDTSRRCAELEKLGLLFVGSGVSGGEEGARHGPSLMPGGHEAAWPLIKPIFQAICAKADGEPCCEWVGDGGAGHFVKMVHNGIEYGDMQLICEAYHIMQSLGLSATQMADEFGKWNSAELDSFLIEITRDILKYKDTKGHLLERIRDTAGQKGTGKWTAIAALQYGVPVTLIGEAVFSRCLSALKQERVHASGVLKGSSVKPSVANVTKFLDDIKHALYCSKIVSYAQGFMLMREAAKENNWRLNYGGIALMWRGGCIIRSVFLGNIKDAYTSQPQLSNLLLDDFFKKAIERGQDSWREVVANSFRWGIPVPALSTALSFYDGYRTAKLPANLLQAQRDYFGAHTYELLGDEGKFHHTNWTGTGGNVSASTYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29H07</accession>
    <accession>A0A6I8UG15</accession>
    <name>Q29H07_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear pore protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Nup93-1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the nucleoporin interacting component (NIC) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354896.1">
        <property type="nucleotide sequence ID" value="XM_001354860.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29H07"/>
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        <property type="interactions" value="2984"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29H07"/>
    <dbReference type="GeneID" id="4814743"/>
    <dbReference type="KEGG" id="dpo:4814743"/>
    <dbReference type="eggNOG" id="KOG2168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011846_1_0_1"/>
    <dbReference type="InParanoid" id="Q29H07"/>
    <dbReference type="OMA" id="RPHAVHM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070809">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007231">
        <property type="entry name" value="Nucleoporin_int_Nup93/Nic96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225:SF4">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93 NUCLEOPORIN NUP93 DEAD EYE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04097">
        <property type="entry name" value="Nic96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="3 5" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="764"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="771"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001354896.1"/>
        </source>
    </evidence>
    <sequence length="828" mass="94678" checksum="AAB92C0E68801D5E" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q29H64</accession>
    <accession>A0A6I8UG17</accession>
    <name>Q29H64_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Inorganic phosphate cotransporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MFS10</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354839.2">
        <property type="nucleotide sequence ID" value="XM_001354803.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29H64"/>
    <dbReference type="FunCoup" id="Q29H64">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="GeneID" id="4814735"/>
    <dbReference type="KEGG" id="dpo:4814735"/>
    <dbReference type="eggNOG" id="KOG2532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_0_1"/>
    <dbReference type="InParanoid" id="Q29H64"/>
    <dbReference type="OMA" id="RHIFGLM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078122">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17318">
        <property type="entry name" value="MFS_SLC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000445">
        <property type="entry name" value="putative inorganic phosphate cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000067">
        <property type="entry name" value="sialin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF399">
        <property type="entry name" value="FI19708P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="86"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001354839.2"/>
        </source>
    </evidence>
    <sequence length="581" mass="63862" checksum="EDF000D87B984D66" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q29J11</accession>
    <accession>A0A6I8UHE6</accession>
    <name>Q29J11_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">trypsin</fullName>
            <ecNumber evidence="11">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4815539</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001355433.1">
        <property type="nucleotide sequence ID" value="XM_001355397.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29J11"/>
    <dbReference type="FunCoup" id="Q29J11">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29J11"/>
    <dbReference type="MEROPS" id="S01.A35"/>
    <dbReference type="GeneID" id="4815539"/>
    <dbReference type="KEGG" id="dpo:4815539"/>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_0_1"/>
    <dbReference type="InParanoid" id="Q29J11"/>
    <dbReference type="OMA" id="MMCAGRL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073277">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29J11">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000111">
        <property type="entry name" value="Blast:Serine protease nudel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="35"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355433.1"/>
        </source>
    </evidence>
    <sequence length="304" mass="33123" checksum="A257387C9A87FEC5" modified="2006-04-04" version="1">MGHGTLFRLELELGCFVWLLICGSVAAQTETQPRIINGSVAKVEETRHLVSIRLRRHDNNFGSGHICGGALIGVNKVLTAAHCLYNNGRKRYRRPSELIVVMGTLNRYERLNSTIVCEVSSMAYMNTFSPDSMRDDVGVLFLRTGLIRPDPSSVAPIQLARQVTPPGRVCQVAGWGRTEQSTLSNILLTANVSTIRQQTCQSIYKSGLLPGMMCAGRLQGGTDSCQGDSGGPLVHEGRVIGVVSWGYGCAEPGLPGVYVDVQYYQQWIDERSGVARSRTSPGLGLSFSLLFGFLTFTASTLVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JK9</accession>
    <accession>A0A6I8UGR2</accession>
    <name>Q29JK9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Or2a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355235.2">
        <property type="nucleotide sequence ID" value="XM_001355199.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JK9"/>
    <dbReference type="FunCoup" id="Q29JK9">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="GeneID" id="4816069"/>
    <dbReference type="KEGG" id="dpo:4816069"/>
    <dbReference type="eggNOG" id="ENOG502SAW0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033399_8_1_1"/>
    <dbReference type="InParanoid" id="Q29JK9"/>
    <dbReference type="OMA" id="CYFGDRM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355235.2"/>
        </source>
    </evidence>
    <sequence length="389" mass="44036" checksum="D0970CEECDB6F637" modified="2008-10-14" version="2">MQPGSENQPELNTHSAVDYHWRVWQLTGLIRPAGISKSLYRTYAIGLNLMVTLLFPLSLLARLILTRSMKELCENLTITITDITANLKFGNVYLVRRHLGEIRSLIQQLDCRALAICDRDELCALVQAVTTARNTFRTFAWIFVCGTSLSCVRVALARSRQLLYPAWFGVDWKNSNEAFVGIYVYQLFGLIVQAVQNCASDSYPPAYLCLLTGHMRALEVRVARIGCECASGQQSYDQLLACIQDLTLIHRMHTIIQQILSVPCMAQFACSVAVQCTVAMHFLYVADADDRLAMILSVIFFVAVTLEVFVICYFGEKMRTQSEALCNAFYACNWVEQLPRFRRDLLFTLARAQRPSLILAGSYIPLTLETFKEVMHFAYSAFTLLLRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MS3</accession>
    <accession>A0A6I8UJK1</accession>
    <name>Q29MS3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4816785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356556.2">
        <property type="nucleotide sequence ID" value="XM_001356520.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MS3"/>
    <dbReference type="STRING" id="46245.Q29MS3"/>
    <dbReference type="GeneID" id="4816785"/>
    <dbReference type="KEGG" id="dpo:4816785"/>
    <dbReference type="eggNOG" id="KOG3868">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_662718_0_0_1"/>
    <dbReference type="InParanoid" id="Q29MS3"/>
    <dbReference type="OMA" id="DINCDVC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078868">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MS3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356556.2"/>
        </source>
    </evidence>
    <sequence length="420" mass="47870" checksum="A1FB895C22DC1074" modified="2008-10-14" version="2">MYRWLCVVMIIKLVWAIFPPSTPPMLIWGLDTPPPQTIFKYMKTFDLVRMLKPIHKDYMIVVYLATELTAKDINCPSCYPMMSQILPVRFYSQVEKPIRALDHISRVSISPGLVWHVLPADGTEINLAKEIPCEIGRIHCFNFGDRNLPAHDATIAAVLDELKSCRVIHIYTAFEEESRALKRRRYYSTSSMRHSHNEDTNVVFTGDVGQKADPTKLTILRHELAIVGVLKIILAEEVKVGHEVHYNRTYVELKTGNESLQVGLVGSHGVNEGFVIVLNTHMGYMLIESLPVGGSWQITRIIFNINVTYYPRELIFFSLKNSLCCQSITAYSDEGARLSLYSFHLDVMTEASDSGFNPDYKPKPCWHCNKYISSVLSQSIFALGLLIFVLSVGFIMLWDIGRNRFVQNIHDPDLHIKTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PH4</accession>
    <accession>A0A6I8UL91</accession>
    <name>Q29PH4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aldehyde dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="4">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4818031</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001357250.1">
        <property type="nucleotide sequence ID" value="XM_001357214.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PH4"/>
    <dbReference type="SMR" id="Q29PH4"/>
    <dbReference type="FunCoup" id="Q29PH4">
        <property type="interactions" value="1276"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29PH4"/>
    <dbReference type="GeneID" id="4818031"/>
    <dbReference type="KEGG" id="dpo:4818031"/>
    <dbReference type="eggNOG" id="KOG2450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_1_1"/>
    <dbReference type="InParanoid" id="Q29PH4"/>
    <dbReference type="OMA" id="GQLIMQY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077672">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07141">
        <property type="entry name" value="ALDH_F1AB_F2_RALDH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000029">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="49"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001357250.1"/>
        </source>
    </evidence>
    <sequence length="521" mass="57000" checksum="D757E30717E86AB3" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PJ6</accession>
    <accession>A0A6I8UL71</accession>
    <name>Q29PJ6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase B1, membrane-associated</fullName>
            <ecNumber evidence="5">3.1.1.3</ecNumber>
            <ecNumber evidence="4">3.1.1.4</ecNumber>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Lysophospholipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Phospholipase A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Phospholipase B/lipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Triacylglycerol lipase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="48" type="primary">LOC4817841</name>
    </gene>
    <organism evidence="47 48">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="48" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="48">MV-25-SWS-2005</strain>
            <tissue evidence="48">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="24">Calcium-independent membrane-associated phospholipase that catalyzes complete diacylation of phospholipids by hydrolyzing both sn-1 and sn-2 fatty acyl chains attached to the glycerol backbone (phospholipase B activity). Has dual phospholipase and lysophospholipase activities toward diacylphospholipids. Preferentially cleaves sn-2 ester bonds over sn-1 bonds. Acts as a lipase toward glycerolipid substrates. Hydrolyzes fatty acyl chains of diacylglycerols with preference for the sn-2 position and of triacylglycerols with not positional selectivity. May also hydrolyze long chain retinyl esters such as retinyl palmitate. May contribute to digestion of dietary phospholipids, glycerolipids and retinoids, facilitating lipid absorption at the brush border.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:40924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + 2 H2O = sn-glycerol 3-phosphocholine + 2 hexadecanoate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:40975"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:40976"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>1,3-di-(9Z-octadecenoyl)-glycerol + H2O = 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39939"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:39940"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1,3-dihexadecanoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40983"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75688"/>
            <dbReference type="ChEBI" id="CHEBI:77619"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:40984"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40979"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75585"/>
            <dbReference type="ChEBI" id="CHEBI:75688"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:40980"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38487"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:38488"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>1-O-hexadecyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40915"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:34112"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:40916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:41112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-3-octadecanoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41103"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
            <dbReference type="ChEBI" id="CHEBI:77624"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:41104"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-3-octadecanoyl-sn-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41107"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75558"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:41108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40811"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:40812"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40971"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
            <dbReference type="ChEBI" id="CHEBI:76084"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:40972"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40919"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72841"/>
            <dbReference type="ChEBI" id="CHEBI:75158"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:40920"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38779"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:38780"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40911"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73007"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:40912"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycerol + H2O = 1-octadecanoyl-sn-glycerol + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40927"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:75550"/>
            <dbReference type="ChEBI" id="CHEBI:77097"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:40928"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = 3-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
            <dbReference type="ChEBI" id="CHEBI:75938"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:42605"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>2-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38491"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="46">
            <dbReference type="Rhea" id="RHEA:38492"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36702"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:36232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="5"/>
    <dbReference type="EC" id="3.1.1.4" evidence="4"/>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001357228.2">
        <property type="nucleotide sequence ID" value="XM_001357192.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PJ6"/>
    <dbReference type="STRING" id="46245.Q29PJ6"/>
    <dbReference type="GeneID" id="4817841"/>
    <dbReference type="KEGG" id="dpo:4817841"/>
    <dbReference type="eggNOG" id="KOG3670">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038975_1_0_1"/>
    <dbReference type="InParanoid" id="Q29PJ6"/>
    <dbReference type="OMA" id="HRVGCHC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070771">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01824">
        <property type="entry name" value="Phospholipase_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008265">
        <property type="entry name" value="Lipase_GDSL_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035547">
        <property type="entry name" value="Phospholipase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038885">
        <property type="entry name" value="PLB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325:SF31">
        <property type="entry name" value="GH22081P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325">
        <property type="entry name" value="PHOSPHOLIPASE B, PLB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01098">
        <property type="entry name" value="LIPASE_GDSL_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="47" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00031485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00033022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00045916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00048362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00048374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00048454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00048613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00048699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00048869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00048872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00048939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00049363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00049372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="ARBA" id="ARBA00049461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="47">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="48">
        <source>
            <dbReference type="RefSeq" id="XP_001357228.2"/>
        </source>
    </evidence>
    <sequence length="448" mass="51564" checksum="BB9A69C2F867DE82" modified="2008-10-14" version="2">MSNIGCFLWLLSLLLLMSPISTSNSRRIRGENRSDRLLADIGVHAQRYDPRKLDNGLQQYTTIDRDLRHLFVGTRRVLLNFALRNIEDLTNRNMREGKIQLPISKRKPFPCPVNNTRSESPPTSVERLRPGDIDIIAAFGDSLSAGNGILSNNIADMLNEFRALSFSGGGLENWRKYVTLPNILKIFNPKLYGFSVANSLVVNHPSSRFNIAEPMIMSRDLPFQARVLIDLLHRDPNVDMQRHWKLLTVYVGNNDLCSDMCHWDEPQALLDQHARDLRQAFRLLRDNVPRLLINLIVVPNILLTLKAMKQIPFQCFVVHRVGCHCLMNDRLNRTQQSQLMDTLQRWQQVDLEVARLAEFHREDFAVVAHPMLANITAPRLKNGLTDWRFFSHDCFHFSQRGHAIISNMLWNSMLLPDDRKPRPFTIPGLFESTVCPSEEQPYFAVRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M090</accession>
    <accession>A0A6I8UD88</accession>
    <name>Q2M090_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glutamate receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ir76b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353533.1">
        <property type="nucleotide sequence ID" value="XM_001353497.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M090"/>
    <dbReference type="FunCoup" id="Q2M090">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M090"/>
    <dbReference type="GeneID" id="4813828"/>
    <dbReference type="KEGG" id="dpo:4813828"/>
    <dbReference type="eggNOG" id="KOG1052">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025094_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M090"/>
    <dbReference type="OMA" id="QEANKWA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080309">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050907">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000173">
        <property type="entry name" value="glutamate receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000277">
        <property type="entry name" value="glutamate receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF24">
        <property type="entry name" value="IONOTROPIC RECEPTOR 60A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="9">
        <location>
            <begin position="209"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353533.1"/>
        </source>
    </evidence>
    <sequence length="639" mass="71739" checksum="7D29865F31728EF1" modified="2006-02-21" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0P4</accession>
    <accession>A0A6I8UCQ4</accession>
    <name>Q2M0P4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EMC4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the EMC4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353379.1">
        <property type="nucleotide sequence ID" value="XM_001353343.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0P4"/>
    <dbReference type="FunCoup" id="Q2M0P4">
        <property type="interactions" value="2247"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0P4"/>
    <dbReference type="GeneID" id="4813482"/>
    <dbReference type="KEGG" id="dpo:4813482"/>
    <dbReference type="eggNOG" id="KOG3318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098404_0_1_1"/>
    <dbReference type="InParanoid" id="Q2M0P4"/>
    <dbReference type="OMA" id="FMMWMVG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070841">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017207">
        <property type="entry name" value="UCP017207_TM-p85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353379.1"/>
        </source>
    </evidence>
    <sequence length="166" mass="18415" checksum="5943678F0D263E2E" modified="2006-02-21" version="1">MSAKQNSKKFKWALDFNGSKNSEIPSPLGYNPSAVVNQTEVVRDQRLVIKKSWDLALGPLKNIPMNLFIMYMSGNSISIFPIMMVGMMLVRPIKAMFTTQVTSKMAEGAQGTGQRIVYFLGNLANVALALYKCHSMGLLPTHASDWLAFVQPQTRLEYYGGGISFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0V3</accession>
    <accession>A0A6I8UCL8</accession>
    <name>Q2M0V3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Retinoblastoma-binding protein 5 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Rbbp5</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353320.2">
        <property type="nucleotide sequence ID" value="XM_001353284.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0V3"/>
    <dbReference type="SMR" id="Q2M0V3"/>
    <dbReference type="FunCoup" id="Q2M0V3">
        <property type="interactions" value="2167"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0V3"/>
    <dbReference type="GeneID" id="4813483"/>
    <dbReference type="KEGG" id="dpo:4813483"/>
    <dbReference type="eggNOG" id="KOG1273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032142_2_2_1"/>
    <dbReference type="InParanoid" id="Q2M0V3"/>
    <dbReference type="OMA" id="DYEDDIM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078986">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0V3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037850">
        <property type="entry name" value="RBBP5/Swd1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040:SF1">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="427"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353320.2"/>
        </source>
    </evidence>
    <sequence length="488" mass="54297" checksum="5062B0CE78616D7A" modified="2008-10-14" version="2">MNLELLESFGQNYPEEFDGSLDCISLAVTCAFNKYGTLLAVGCNDGRIVIWDFLTRGIAKIISAHVHPVCSLSWTRNGHKLLSASTDNNVCIWDVLTGELEHKYRFPSPVLKVQFDPRNDNRLLVCPMRYAAVLVEIGGSHRRLPLDSDGDLNIVASFDRRGKHIYTGNAKGKILVLDVETFEVVASFRIIVGTSSATAVKSIEFARRGDAFLINTSDRVIRVYDSKEIIALGRDGEPEPIQKLQDLVNKTTWKKCCFSGDGEYICAGSARQHALYIWEKSIGNLVKILHGTKGELLLDVVWHPVRPIIASISSGLVSIWAQNQVENWSAFAPDFKELDENVEYEERESEFDIADEDKSVDLNADAQQDEEIEVDVQKVEPVAAFCSSDEEGEDENALQFLPMAPEVEDPEDGWAAQDGLEASTVLLSNDPHDYDDDIMASKRRRVQHYDVSLPDAPTDETHPLISSKASKDKQQPVGGKKATGRTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NXR4</accession>
    <name>A0A0R3NXR4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">F-box-like/WD repeat-containing protein TBL1XR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC26532259</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat EBI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239493.1">
        <property type="nucleotide sequence ID" value="XM_033383602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NXR4"/>
    <dbReference type="STRING" id="46245.A0A0R3NXR4"/>
    <dbReference type="KEGG" id="dpo:26532259"/>
    <dbReference type="InParanoid" id="A0A0R3NXR4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000001">
        <property type="entry name" value="F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002234">
        <property type="entry name" value="F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033239493.1"/>
        </source>
    </evidence>
    <sequence length="560" mass="60075" checksum="EC49B199C2005E1B" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P2K6</accession>
    <accession>A0A6I8VJV3</accession>
    <name>A0A0R3P2K6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 9">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">UGP</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19890"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_015042489.1">
        <property type="nucleotide sequence ID" value="XM_015187003.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P2K6"/>
    <dbReference type="SMR" id="A0A0R3P2K6"/>
    <dbReference type="FunCoup" id="A0A0R3P2K6">
        <property type="interactions" value="1453"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P2K6"/>
    <dbReference type="GeneID" id="4814017"/>
    <dbReference type="InParanoid" id="A0A0R3P2K6"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078133">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 9 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015042489.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="58832" checksum="CAD3606C5AE75045" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P3Z1</accession>
    <accession>A0A6I8VKS7</accession>
    <name>A0A0R3P3Z1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proton-coupled amino acid transporter-like protein CG1139 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4813316</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015042984.1">
        <property type="nucleotide sequence ID" value="XM_015187498.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P3Z1"/>
    <dbReference type="FunCoup" id="A0A0R3P3Z1">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P3Z1"/>
    <dbReference type="GeneID" id="4813316"/>
    <dbReference type="InParanoid" id="A0A0R3P3Z1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079510">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P3Z1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF460">
        <property type="entry name" value="PROTON-COUPLED AMINO ACID TRANSPORTER 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="5">
        <location>
            <begin position="135"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015042984.1"/>
        </source>
    </evidence>
    <sequence length="548" mass="59737" checksum="571C0170FD690560" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P4Z7</accession>
    <accession>A0A6I8VKH9</accession>
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    <protein>
        <submittedName>
            <fullName evidence="13">Dynein intermediate chain 3, ciliary isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Dnai2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043280.1">
        <property type="nucleotide sequence ID" value="XM_015187794.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P4Z7"/>
    <dbReference type="SMR" id="A0A0R3P4Z7"/>
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        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P4Z7"/>
    <dbReference type="GeneID" id="4813119"/>
    <dbReference type="eggNOG" id="KOG1587">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3P4Z7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000584">
        <property type="entry name" value="Dynein intermediate chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000975">
        <property type="entry name" value="Dynein, axonemal, intermediate chain 2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF7">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
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    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015043280.1"/>
        </source>
    </evidence>
    <sequence length="585" mass="66628" checksum="81D2F117995D693E" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P711</accession>
    <accession>A0A6I8VIR0</accession>
    <name>A0A0R3P711_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cuticle protein 16.5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6901021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042199.1">
        <property type="nucleotide sequence ID" value="XM_015186713.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P711"/>
    <dbReference type="STRING" id="46245.A0A0R3P711"/>
    <dbReference type="GeneID" id="6901021"/>
    <dbReference type="InParanoid" id="A0A0R3P711"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243737">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022727">
        <property type="entry name" value="Cuticle_C1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39068:SF4">
        <property type="entry name" value="AGAP000382-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39068">
        <property type="entry name" value="LARVAL/PUPAL CUTICLE PROTEIN H1C-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015042199.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="30617" checksum="8DB2068334B8DBDD" modified="2016-01-20" version="1">MEEGSGGRGPTRPKHRQQHQYQQSECERAPHTKRCVSFKVILCVAAMAIACAQAKPGGPAAYSISAPSVDHASVGNTQEHTVKGHYGQSSQSDYASQVQTAHSQSHVQRSSISNDAGLAPAAHYAAPATHAIAAPAIGASYALGVGHTAPAQIQGLAYASHGYAAAPTVAYGGHYGPQYGGGHYAATAPALQLSGVAVGHYGHIGHLGLGAGLGYGYGGYSSGPALSHGYAQVAAAPAQVVKYAATPAYAPGIIGHGYAPAAYAAPAYAAPAYATHLGGPVLKAAVAAPALVHTSVSGHGIHYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBI3</accession>
    <name>A0A6I8UBI3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell division cycle protein 48 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4811910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352857.4">
        <property type="nucleotide sequence ID" value="XM_001352821.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBI3"/>
    <dbReference type="KEGG" id="dpo:4811910"/>
    <dbReference type="InParanoid" id="A0A6I8UBI3"/>
    <dbReference type="OMA" id="CAERFIT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071387">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001921">
        <property type="entry name" value="AAA ATPase domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF194">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="222"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="493"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352857.4"/>
        </source>
    </evidence>
    <sequence length="729" mass="80982" checksum="D4D24379A190DF97" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBJ0</accession>
    <name>A0A6I8UBJ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Hemocytin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Hml</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine protease inhibitor-like (TIL domain-containing) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the thrombospondin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352900.4">
        <property type="nucleotide sequence ID" value="XM_001352864.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UBJ0">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812368"/>
    <dbReference type="InParanoid" id="A0A6I8UBJ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000055">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012111">
        <property type="entry name" value="Hml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF386">
        <property type="entry name" value="HEMOLECTIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23244">
        <property type="entry name" value="VWF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036569">
        <property type="entry name" value="Hml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026338497" evidence="11">
        <location>
            <begin position="21"/>
            <end position="3840"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="188"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="17">
        <location>
            <begin position="472"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="17">
        <location>
            <begin position="838"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="17">
        <location>
            <begin position="1325"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="16">
        <location>
            <begin position="1848"/>
            <end position="1915"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="13">
        <location>
            <begin position="2064"/>
            <end position="2217"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="13">
        <location>
            <begin position="2245"/>
            <end position="2394"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="17">
        <location>
            <begin position="2694"/>
            <end position="2874"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="17">
        <location>
            <begin position="3026"/>
            <end position="3224"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="15">
        <location>
            <begin position="3382"/>
            <end position="3450"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="12">
        <location>
            <begin position="3751"/>
            <end position="3811"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="3751"/>
            <end position="3805"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="3755"/>
            <end position="3807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001352900.4"/>
        </source>
    </evidence>
    <sequence length="3840" mass="426211" checksum="10423A37393E6B8F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBN9</accession>
    <name>A0A6I8UBN9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Mkp3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001352925.4">
        <property type="nucleotide sequence ID" value="XM_001352889.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBN9"/>
    <dbReference type="FunCoup" id="A0A6I8UBN9">
        <property type="interactions" value="536"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812295"/>
    <dbReference type="InParanoid" id="A0A6I8UBN9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072796">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14566">
        <property type="entry name" value="DSP_MKP_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000054">
        <property type="entry name" value="Dual specificity phosphatase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000157">
        <property type="entry name" value="Protein-tyrosine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="9">
        <location>
            <begin position="22"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="213"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="278"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="5">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001352925.4"/>
        </source>
    </evidence>
    <sequence length="464" mass="50579" checksum="6DA1AF93E3B1CF91" modified="2020-08-12" version="1">MPETEHESCSKEWLQVQLRSLDAKELILLDCRGSHEYSESHIRGAVNLCIPSIVLRRLATGKIDLASTIKSPDLKDRIQTGYKLCLFILYNGEGVAGQQDVAGAGMGANDSIINILHRRLKQDGCRVVALQDGFSSFRLAFPEWCEDDSQAQNKEMESSRNVQTDQLMGLRSLRISTPHSDSACSSSAESSDCESTTHHHHHHHHHHHSYNEAPVEIIPGLLFLGNVTHSGDSKALQKYNIKYVLNVTPDLPNEFEKSGIIKYLQIPITDHYSQDLAMHFPDAIQFIEEARSANSAVLVHCLAGVSRSVTVTLAYLMHTRGLSLNDAFMMVRDRKPDVSPNFHFMQQLQSFESQLRLSPGDGQPMDEGNCGGMMGGMGGPPVGLGLAPVGSSVSNSVLMANPSVVASRCGGRGSKFSCNCIAADCKCMQTGGFMAAHLAKATGVSPDSGIEFDRWTPSSDTGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCM4</accession>
    <name>A0A6I8UCM4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histone acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">Gcn5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001353404.2">
        <property type="nucleotide sequence ID" value="XM_001353368.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCM4"/>
    <dbReference type="FunCoup" id="A0A6I8UCM4">
        <property type="interactions" value="513"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813891"/>
    <dbReference type="InParanoid" id="A0A6I8UCM4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCM4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016376">
        <property type="entry name" value="GCN5/PCAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009464">
        <property type="entry name" value="PCAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06466">
        <property type="entry name" value="PCAF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003048">
        <property type="entry name" value="Histone_acetylase_PCAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 17" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="20">
        <location>
            <begin position="478"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="19">
        <location>
            <begin position="718"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="372"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="16">
        <location>
            <position position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR003048-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001353404.2"/>
        </source>
    </evidence>
    <sequence length="810" mass="92112" checksum="CC5E526CAF83A18D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDK0</accession>
    <name>A0A6I8UDK0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353678.3">
        <property type="nucleotide sequence ID" value="XM_001353642.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDK0"/>
    <dbReference type="KEGG" id="dpo:4813023"/>
    <dbReference type="InParanoid" id="A0A6I8UDK0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDK0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353678.3"/>
        </source>
    </evidence>
    <sequence length="149" mass="16990" checksum="9AA6ACC54FF8F531" modified="2020-08-12" version="1">MPESSRCRLVILIVAAAYIIGVVIQSQFHCEPRKWSPSSWDGAYGSLFSTAFESRCLAHKLENKFRQYQEAVFRHAENCTIESEIGDSGVQYLRISCLVDDPAPGGRPRLQSGDHFRNLFSKRRIPNPLMRKWTLRVPRDAVKRLGEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEN4</accession>
    <name>A0A6I8UEN4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dehydrogenase/reductase SDR family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Dhrs4</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354278.2">
        <property type="nucleotide sequence ID" value="XM_001354242.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEN4"/>
    <dbReference type="FunCoup" id="A0A6I8UEN4">
        <property type="interactions" value="706"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814162"/>
    <dbReference type="InParanoid" id="A0A6I8UEN4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UEN4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE (SDR FAMILY) MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354278.2"/>
        </source>
    </evidence>
    <sequence length="319" mass="33935" checksum="49AD2842B55245A4" modified="2020-08-12" version="1">MFHLGKQFLGRAPKLHLSASTTVSGAGVQSATRNLNQTNNKPDQKLVNHNLTHKRLSSSSQSSSVNQMKRLAGKVAVVTASTDGIGFAIAKRLAEDGAAVVISSRKQKNVDNALAELRKLNLNVHGLKCHVSEPQDRKQLFDETISKYGKLNILVSNAATNPAVGGVLDCDEKVWDKIFDVNVKSSYLLAKEALPFLRQEKGSSIVFVSSIAGYDAFELLGAYSVSKTALIGLTKAAAKDLAPEGIRVNCLAPGIIKTKFSKALYEDESANDAALMKIPMGRLGTSEEMAGVVSFLVSEDAAYITGEAIVAGGGMSARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UES3</accession>
    <name>A0A6I8UES3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease H2 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ribonuclease HI subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4814205</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase H2 subunit B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354323.3">
        <property type="nucleotide sequence ID" value="XM_001354287.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UES3"/>
    <dbReference type="FunCoup" id="A0A6I8UES3">
        <property type="interactions" value="634"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814205"/>
    <dbReference type="InParanoid" id="A0A6I8UES3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UES3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
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            <end position="105"/>
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            <begin position="1"/>
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            <begin position="1"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00009823"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00011277"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00033464"/>
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            <dbReference type="Pfam" id="PF09468"/>
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            <dbReference type="Pfam" id="PF17745"/>
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        <source>
            <dbReference type="RefSeq" id="XP_001354323.3"/>
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    <sequence length="340" mass="37909" checksum="797B06C0382B11E3" modified="2020-08-12" version="1">MSKKTTRSAKPKPDPDANIDTAAKKSTVAGLKKVLYISEKILPDDNQLLRLDRFYHPGKGKDALFLTNPDGQIMELLEYAEPRRSWLINSEICANGRVYMTMPMDTTLLAIHHLRKHCAQRAMSLDNIAVHEASTSRFLTQFIDSNGLKCVADVKTSGDLTFYKYNQERTLAWLALKTRHVAEILKQKQIHCGHSAQSQNFVRSEKLSDNVVSEMDYGRMACDIVGRYLDSDLHDQLIGYLHIPSEIQAIVEEKTAAQKRKSQVGLEGSNSKKIKLNSHDSDAASKLKLSGLLDSDNGKDCITSPPTMEPLKERSMTAKEKALAKGAKGTKSIASFFKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFF9</accession>
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            <fullName evidence="8">Histone-lysine N-methyltransferase 2D</fullName>
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        <name evidence="8" type="primary">Lint-O</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="8">MV-25-SWS-2005</strain>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_001354636.3"/>
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    <sequence length="512" mass="56181" checksum="CD305FC6C50EDEF4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFK7</accession>
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        <location>
            <begin position="381"/>
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        <location>
            <begin position="428"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001354711.3"/>
        </source>
    </evidence>
    <sequence length="653" mass="73064" checksum="038F299BBD0A84FC" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8UFM6</accession>
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            <fullName evidence="5">Cyclin-dependent serine/threonine-protein kinase DDB_G0272797/DDB_G0274007</fullName>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        </lineage>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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            <begin position="1"/>
            <end position="25"/>
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    <feature type="chain" id="PRO_5026090433" description="Cyclin-dependent serine/threonine-protein kinase DDB_G0272797/DDB_G0274007" evidence="2">
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            <end position="371"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="198"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="286"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="356"/>
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    </feature>
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        <location>
            <begin position="33"/>
            <end position="42"/>
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        <location>
            <begin position="213"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="249"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354721.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="42124" checksum="C43A0E4493371E68" modified="2020-08-12" version="1">MESKVHCSWPLLLLLLMLALGRVRPENINDTTASEDIDDDEATSPSPSIPETTDRLITGDLRKAARVEQLSPPGSGSASKQEYDVIDVLSATNAVVGKAKSGQTTRIFTTGDVDDTFWMKHLGDDFKHAIHLQVGGTNEEYNRLVNQTQNGIHEEVHHEYLPGAERPDSMQQQQQQPGARYTQRSSPRQQQQQQQGLDGEALAMKQRYLIHQQQQQQQQQQRYGYTQQHAQQYTPRAHTQIHQYPQPHPHPHTHQHQHQQQPHTHRYQRPYPPKAQQQQQQQQQQVRYINHRPPIRTIINSSEDQLHAAQSSPGAQHAAAAAIQAGGGIGRSPDEDEADTFGSQLPFKSPFNDYGSRPTRDLTYLLYRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG90</accession>
    <name>A0A6I8UG90_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein-lysine N-methyltransferase EEF2KMT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4814764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355016.2">
        <property type="nucleotide sequence ID" value="XM_001354980.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UG90"/>
    <dbReference type="FunCoup" id="A0A6I8UG90">
        <property type="interactions" value="1164"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814764"/>
    <dbReference type="InParanoid" id="A0A6I8UG90"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UG90">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF130">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EEF2KMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355016.2"/>
        </source>
    </evidence>
    <sequence length="316" mass="35692" checksum="4C27B259DD88B0B1" modified="2020-08-12" version="1">MTDRFSKMQQQFLCCYPVNKMSWSSVKLPMEWDEQKALIAATCGHPLNERYPVRTSYQEAFIKRLMQLLKDHEELHDDLFSSLCIQKDNNHGKDYAYKHYLLLQPDAHITLRESISFVNEGTTGLCTWEAALALADYLLQHSDVVKGKNIVELGAGTGLIGILLKLPALGLDAGQILLTDGSASCVQLMRENVTLNFPTAPKEAKPQCEQLRWDEVKDFPWHQYAKTDLLLAADVIYDDSQFDALIGALDHLYTSRGNCLEMLLASTVRNVDTLHKFMEQLTVHEYKVTPCANVSACASHFCRDHTAAVQIISIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGF1</accession>
    <name>A0A6I8UGF1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4814564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355101.3">
        <property type="nucleotide sequence ID" value="XM_001355065.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGF1"/>
    <dbReference type="FunCoup" id="A0A6I8UGF1">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814564"/>
    <dbReference type="InParanoid" id="A0A6I8UGF1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019193">
        <property type="entry name" value="UBQ-conj_enz_E2-bd_prot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09814">
        <property type="entry name" value="HECT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355101.3"/>
        </source>
    </evidence>
    <sequence length="488" mass="55693" checksum="D14EE8DA4193565C" modified="2020-08-12" version="1">MLAGVLIEIRPYLNCGQVYIQLHRNDASNMRVVILEQRIYIDDADRGTGKSIDTEIMRVNEFDMDLSSLSGFLLSGKNMSFRFSFNRIAVAVLDKRTVSVPMQPLLLSCCENESLAIQCVGCQAEVAPRRIYTRLREFPNCIVDPNEFFCHGHDHGHGHGHGQDRPHSLLPGEKDLFYGLNNVIIRMVNLAKIVRRGEHLHCQRCMRFLGMAIFNGEAARLWADTVRWLPSAQKSDGLPPAIRHFFQFSTMTELVLRLLNSLWPHVLPSINLTGSRAVLYASPTSQQKQYMLIRVVEPQLRVLRRISGKAPVLRGYYAIKLSYGIYEDCQEEPPDILQRWQAHQDVSQFEISPKMFMELRQRLDRNAEYQPMEWRRSAMEENLVLTYFIYEDEAQPGGSYVASAKLPSSYSSDSDDEQSDPGSVMFGRKRSPSIRRAISLPSVLNGESNRQRLLNWLLESRLNAAKKESGRQRLAELQPNLSDASSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH47</accession>
    <name>A0A6I8UH47_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein b6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">b6</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_001355423.4">
        <property type="nucleotide sequence ID" value="XM_001355387.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UH47"/>
    <dbReference type="FunCoup" id="A0A6I8UH47">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815611"/>
    <dbReference type="InParanoid" id="A0A6I8UH47"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UH47">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF125">
        <property type="entry name" value="B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pentraxin (PTX)" evidence="7">
        <location>
            <begin position="153"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="371"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355423.4"/>
        </source>
    </evidence>
    <sequence length="584" mass="63880" checksum="8E3AB674DA4B45DA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHB0</accession>
    <name>A0A6I8UHB0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Myotubularin-related protein 10-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC4815431</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355510.3">
        <property type="nucleotide sequence ID" value="XM_001355474.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041183.2">
        <property type="nucleotide sequence ID" value="XM_015185697.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHB0"/>
    <dbReference type="KEGG" id="dpo:4815431"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHB0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14537">
        <property type="entry name" value="PTP-MTMR10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022587">
        <property type="entry name" value="MTMR12-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF110">
        <property type="entry name" value="FI17948P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12578">
        <property type="entry name" value="3-PAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="3">
        <location>
            <begin position="261"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="732"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="734"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001355510.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015041183.2"/>
        </source>
    </evidence>
    <sequence length="833" mass="93464" checksum="B21767E720A4E534" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHL7</accession>
    <name>A0A6I8UHL7_DROPS</name>
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        <submittedName>
            <fullName evidence="11">Krueppel-like factor 7</fullName>
        </submittedName>
    </protein>
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        <name evidence="11" type="primary">Klf15</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355642.3">
        <property type="nucleotide sequence ID" value="XM_001355606.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHL7"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kruppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
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    </dbReference>
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        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
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    </dbReference>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="202"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="173"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001355642.3"/>
        </source>
    </evidence>
    <sequence length="315" mass="35164" checksum="2370F6902A469724" modified="2020-08-12" version="1">MEFFATGAFQQLYSELDDECGVSVSVSSDLILTASSDLDYGYAEARGQCDQVPVVDTEYYGIITLDSNNNQSNDYTIGHPEDEDHGLFFDFSDQDLSGCSTAGCSPPELSEWEQRLLDNFVEIPELIDFLPERTPLCTDMCDDFLEESNSNLRLAAPPPPPPAPPMSPSCLEPAPTAPATDAATDTATVPQLSQNAAGERGYLCTFGNCEKLYAKPAHLKAHLRRHLGEKPYACSWPECSWRFSRSDELARHRRSHSGVKPYKCDYCAKCFARSDHLTKHRKVHERRLLAATRLGKLLPDDVYAVRPGRKRKNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHR5</accession>
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        <recommendedName>
            <fullName evidence="7">von Willebrand factor A domain-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">c12.2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Exhibits ATPase activity in vitro.</text>
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        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355666.2">
        <property type="nucleotide sequence ID" value="XM_001355630.3"/>
    </dbReference>
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        <property type="interactions" value="1186"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHR5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01455">
        <property type="entry name" value="vWA_F11C1-5a_type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="von Willebrand factor A domain containing 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="von Willebrand factor A domain containing 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="von Willebrand factor, type A domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="VWF_A"/>
    </dbReference>
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        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21610">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21610:SF9">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="1208"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="691"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1059"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1062"/>
            <end position="1080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001355666.2"/>
        </source>
    </evidence>
    <sequence length="1398" mass="156484" checksum="9AD32888EBD951D9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIB9</accession>
    <name>A0A6I8UIB9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Serine protease 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Jon25Bii</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355939.3">
        <property type="nucleotide sequence ID" value="XM_001355903.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIB9"/>
    <dbReference type="SMR" id="A0A6I8UIB9"/>
    <dbReference type="KEGG" id="dpo:5495509"/>
    <dbReference type="InParanoid" id="A0A6I8UIB9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000025">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000043">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8 12" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026225703" evidence="9">
        <location>
            <begin position="18"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="43"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355939.3"/>
        </source>
    </evidence>
    <sequence length="276" mass="29634" checksum="A4C6ED5FCEB5268C" modified="2020-08-12" version="1">MKLFLAVLALAFACASALPQKAEPVPLKDAVLGSSPGSIEGRITNGYPAYEGKVPYVVGLGFSKSGSGWWCGGSIIGNTWVVTAAHCTHGADSVTIYYGALWRLQAQYTHTVHSGSFRQHSNYNTNNLNNDISLINTPHVDFWHLVNKVELPSYNDRYNTYSGWWALASGWGRTSDNSGVSDYLNCVDSQIIDRSACSSVYGTDVITDHVICTSTPDGKSTCSGDSGGPLVLHEGSKLVGVTSFVASNGCTSGLPDGFTRVTSYLDWIRDHTGISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIT7</accession>
    <name>A0A6I8UIT7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spermatogenesis-associated protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4816391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356178.3">
        <property type="nucleotide sequence ID" value="XM_001356142.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIT7"/>
    <dbReference type="KEGG" id="dpo:4816391"/>
    <dbReference type="InParanoid" id="A0A6I8UIT7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIT7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356178.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="41663" checksum="B8B217C4E82EA56B" modified="2020-08-12" version="1">MTSNKIQKTHSLTGRYSEDAVSVISLYFEQSVSSSFSHIGNDVGTTPQNRVLQLLLDYKLFKAARTIQRFVRGWLCRKMLAKGAAAVTTISRWWRGFRVRQNYYVLMEQRLQKKLLDHYYEQATKIQTLFRGWRTRQFVQDFNGLQLIQMQFAEDLISCLARSLRDVKNNDQLPGVYCLRTDGRCLRIIEDLARTFSYRFHNGRVRASIAKRRSYLEDMRQSFHLSHAYTLTPFPGPGAVLGDCTTHVDITEPGFSKETDFRMQRLILMFEKSKRDPDVVRVQNSLASKHRRDIQTSLKAQKEERIKNFCTFVFHRIASTIGNKRSSVKNYLDELLYNAEEMCCHCQPKLVTQSLCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJE3</accession>
    <name>A0A6I8UJE3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4816904</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356455.4">
        <property type="nucleotide sequence ID" value="XM_001356419.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJE3"/>
    <dbReference type="FunCoup" id="A0A6I8UJE3">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816904"/>
    <dbReference type="InParanoid" id="A0A6I8UJE3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJE3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07066">
        <property type="entry name" value="CRD_FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270:SF57">
        <property type="entry name" value="FI24007P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="12">
        <location>
            <begin position="412"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="276"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="372"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="725"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="818"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="303"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="739"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="752"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="821"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="840"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="858"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="459"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="542"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="594"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="778"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="785"/>
            <end position="803"/>
        </location>
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        <location>
            <begin position="797"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001356455.4"/>
        </source>
    </evidence>
    <sequence length="877" mass="96704" checksum="F8622A5F131CA4FC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJK7</accession>
    <name>A0A6I8UJK7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356571.3">
        <property type="nucleotide sequence ID" value="XM_001356535.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJK7"/>
    <dbReference type="KEGG" id="dpo:4816985"/>
    <dbReference type="InParanoid" id="A0A6I8UJK7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356571.3"/>
        </source>
    </evidence>
    <sequence length="524" mass="59935" checksum="D4E3C0B2BC75A5E8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKM9</accession>
    <name>A0A6I8UKM9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PHD finger protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4817573</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356978.3">
        <property type="nucleotide sequence ID" value="XM_001356942.4"/>
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    <dbReference type="KEGG" id="dpo:4817573"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
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    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_PHD"/>
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    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF150">
        <property type="entry name" value="PHD FINGER PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="120"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="183"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="590"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="944"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="769"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="896"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="774"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="801"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="915"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356978.3"/>
        </source>
    </evidence>
    <sequence length="1005" mass="110109" checksum="2A7DC293B8BDE8EF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULB5</accession>
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            <fullName evidence="2">Nucleoporin NUP35</fullName>
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        <alternativeName>
            <fullName evidence="7">35 kDa nucleoporin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nucleoporin NUP53</fullName>
        </alternativeName>
    </protein>
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        <name evidence="12" type="primary">LOC4818171</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357273.2">
        <property type="nucleotide sequence ID" value="XM_001357237.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8ULB5"/>
    <dbReference type="KEGG" id="dpo:4818171"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULB5">
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    </dbReference>
    <dbReference type="GO" id="GO:0005643">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="InterPro" id="IPR007846">
        <property type="entry name" value="RRM_NUP35_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14605">
        <property type="entry name" value="Nup35_RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51472">
        <property type="entry name" value="RRM_NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM Nup35-type" evidence="10">
        <location>
            <begin position="65"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001357273.2"/>
        </source>
    </evidence>
    <sequence length="248" mass="28271" checksum="446E308E67ECC300" modified="2020-08-12" version="1">MNPISEEPKSPSTDEFLTPVNKERVQQLETVLGEVLKEPEGAPPKEEAAVQETEDTDQKDDSGEQLNKFWIAISGYPFERAYLVYRFFNDIGKIHLKGFTQTNVMYLKYHTTVDCQIALSYDGQKIGYGGEIRVSVKPENPMALKAFLGAIEQMDEVTVATDAGPPMADAVTRTDLPASFFIDMEATIQKKETPNKKEETDQKEEQKLEQSLEPEEVKVEPPVDVVLPKKYGFFQWLKEKISHMFFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULK3</accession>
    <name>A0A6I8ULK3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">wwk</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357349.3">
        <property type="nucleotide sequence ID" value="XM_001357313.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035413.2">
        <property type="nucleotide sequence ID" value="XM_015179927.2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817920"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073653">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULK3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF51">
        <property type="entry name" value="ANOCTAMIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="644"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="695"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="8">
        <location>
            <begin position="296"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="771"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="953"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1084"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1314"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="968"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="984"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1094"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1156"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1314"/>
            <end position="1344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357349.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015035413.2"/>
        </source>
    </evidence>
    <sequence length="1353" mass="149557" checksum="679577501207F7B7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULP7</accession>
    <name>A0A6I8ULP7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sodium channel protein Nach</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ppk22</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357547.2">
        <property type="nucleotide sequence ID" value="XM_001357510.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULP7"/>
    <dbReference type="FunCoup" id="A0A6I8ULP7">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="GeneID" id="4800368"/>
    <dbReference type="KEGG" id="dpo:4800368"/>
    <dbReference type="InParanoid" id="A0A6I8ULP7"/>
    <dbReference type="OMA" id="GTWDNET"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076029">
        <property type="expression patterns" value="Expressed in multicellular organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF253">
        <property type="entry name" value="PICKPOCKET 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001357547.2"/>
        </source>
    </evidence>
    <sequence length="563" mass="64507" checksum="6F004D4F07CF857F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UME5</accession>
    <name>A0A6I8UME5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">bond</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 10">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001357857.1">
        <property type="nucleotide sequence ID" value="XM_001357820.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038730.1">
        <property type="nucleotide sequence ID" value="XM_015183244.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UME5"/>
    <dbReference type="GeneID" id="4800612"/>
    <dbReference type="KEGG" id="dpo:4800612"/>
    <dbReference type="OMA" id="MTTYMKI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079954">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF103">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001357857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015038730.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="36797" checksum="6E06952A0B32A245" modified="2020-08-12" version="1">MTTYMKIFEERISGLSKGVDETVDSWFLMSSPAPVFGVVLLYLAFILKIGPEFMKNRKPMDLKRIMVFYNAFQVCYSIWMCRTSIQESNVISSIFSKKCEINRTREQNLALYSGAWFYFFSKIIDLLDTTFFVLRKKNNQVSFLHVYHHTITVLFSWGYLKYAPGEQGVIIGILNSGVHIMMYFYYMVAAMGPQYQKYLWWKKYMTSIQLIQFVLILGYMLAVGAKGCNMPKTLTFFFVGNTVIFLYLFGNFYRKTYNKNKAIDGSSRTGSSLAQSALRAAGGMGCMPTQTQITSQSQGQVQPAKAYIDLNNNSVKPLKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMP8</accession>
    <name>A0A6I8UMP8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isochorismatase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4800730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357950.2">
        <property type="nucleotide sequence ID" value="XM_001357913.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMP8"/>
    <dbReference type="FunCoup" id="A0A6I8UMP8">
        <property type="interactions" value="1022"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800730"/>
    <dbReference type="InParanoid" id="A0A6I8UMP8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd01012">
        <property type="entry name" value="YcaC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.850:FF:000001">
        <property type="entry name" value="Isochorismatase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050993">
        <property type="entry name" value="Isochorismatase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119:SF17">
        <property type="entry name" value="ISOCHORISMATASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="21"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357950.2"/>
        </source>
    </evidence>
    <sequence length="207" mass="23449" checksum="5D518F0D82BD3482" modified="2020-08-12" version="1">MRRAIGSTLYRLQPEKTLFMQCDIQEKFKPAIPLMIALIENTKKLLAAGKILKVPLLVTEQYPERLGKTISELDIQHACANIPKTKFSMIVEPIKKNMVDIFSGKPQTVVLFGLETHICVEQTAFDLINNKVDVWLVADCCASRLNQDRDLALERLRDIGCVITSAESVMFNLLNSKDHQAFKQVSALVRKVSADVQLWQRTKPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN44</accession>
    <name>A0A6I8UN44_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Muscle-specific homeobox protein tinman</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tin</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358064.3">
        <property type="nucleotide sequence ID" value="XM_001358027.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN44"/>
    <dbReference type="FunCoup" id="A0A6I8UN44">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800866"/>
    <dbReference type="InParanoid" id="A0A6I8UN44"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055007">
        <property type="term" value="P:cardiac muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035050">
        <property type="term" value="P:embryonic heart tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000532">
        <property type="entry name" value="C. Elegans Homeobox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF41">
        <property type="entry name" value="MUSCLE-SPECIFIC HOMEOBOX PROTEIN TINMAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="308"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="310"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="261"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="385"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="398"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358064.3"/>
        </source>
    </evidence>
    <sequence length="423" mass="47050" checksum="6CDDB855ACC43FBC" modified="2020-08-12" version="1">MLQHHQQQAQVPQSGGYYDYNQSPSPGSLTNADALNTTPFSVKDILNMVNQTEAYEGAYGHLDSAAAASAFYGAGEYQQHLHQHPHQYQQQQQDLPAASQQQLHHHLVEHQEDATTSSSLSPLLPPPPHGHHQLYGSYQDYGMPAHMFQHHHGHPHQSFQPAASAYNMSATQFYAGATAPSYQSPATYNYNYAGAGDAYPQHSPAVTIKSEYIPTPYVTPSPTLDLNSSAEVESLAPAQKLCGNPLSQRLLETVSNSASLRSIHSTDEGAKRKDNSQVTSSRSELRKNSGSGSNHSSSINSNGSSKPRMKRKPRVLFSQAQVLELECRFRLKKYLTGSEREIIAQKLNLSATQVKIWFQNRRYKSKRGDIDCEGIAKHLKLKAEPLHSPTSLPPPMPNHMMWPPTVQHSQQQHHQQQQLPHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP11</accession>
    <name>A0A6I8UP11_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Choline O-acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ChAT</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.6" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001358450.5">
        <property type="nucleotide sequence ID" value="XM_001358413.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP11"/>
    <dbReference type="FunCoup" id="A0A6I8UP11">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="GeneID" id="4801339"/>
    <dbReference type="KEGG" id="dpo:4801339"/>
    <dbReference type="InParanoid" id="A0A6I8UP11"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071621">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004102">
        <property type="term" value="F:choline O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008292">
        <property type="term" value="P:acetylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000001">
        <property type="entry name" value="Carnitine O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000011">
        <property type="entry name" value="Choline O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF14">
        <property type="entry name" value="CHOLINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="9">
        <location>
            <begin position="105"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358450.5"/>
        </source>
    </evidence>
    <sequence length="735" mass="82636" checksum="D82869CC32E38124" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ39</accession>
    <name>A0A6I8UQ39_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Probable multidrug resistance-associated protein lethal(2)03659</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">Cftr</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <strain evidence="12">MV2-25</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359115.3">
        <property type="nucleotide sequence ID" value="XM_001359078.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037638.2">
        <property type="nucleotide sequence ID" value="XM_015182152.2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802136"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000014">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000482">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF415">
        <property type="entry name" value="FI20190P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="811"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="866"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1049"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1076"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="213"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="493"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="814"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="1141"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="722"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1370"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="731"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="767"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1377"/>
            <end position="1398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359115.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015037638.2"/>
        </source>
    </evidence>
    <sequence length="1413" mass="157865" checksum="247F799ED65A9B4A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQF3</accession>
    <name>A0A6I8UQF3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EMC2A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EMC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359073.1">
        <property type="nucleotide sequence ID" value="XM_001359036.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQF3"/>
    <dbReference type="SMR" id="A0A6I8UQF3"/>
    <dbReference type="FunCoup" id="A0A6I8UQF3">
        <property type="interactions" value="1002"/>
    </dbReference>
    <dbReference type="GeneID" id="4802085"/>
    <dbReference type="KEGG" id="dpo:4802085"/>
    <dbReference type="InParanoid" id="A0A6I8UQF3"/>
    <dbReference type="OMA" id="MSDQEGW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074580">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000478">
        <property type="entry name" value="GG16802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055217">
        <property type="entry name" value="TPR_EMC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22890">
        <property type="entry name" value="TPR_EMC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="EMC2 TPR-like" evidence="5">
        <location>
            <begin position="89"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359073.1"/>
        </source>
    </evidence>
    <sequence length="293" mass="33734" checksum="4256D48DA2E3029F" modified="2020-08-12" version="1">MSLNYEEMSWSDVRDQFRKWREETGRHSEEVVQLWVAVLEDKVHKTGNERHLILEQVIIAALDTAQFDIATKCTKQLSLEFPGSLRVMKFKAMRYEALEQYDEADDVLDAIIAKDETNAAPRKRKIAILKARGRRVEAIKELNDYLKKFMSDQEAWHELCSMYLAEGEYGKAAFCMEEVLLHNPHSHLIHQRLAEIRYTMGGVENVEAARTYYSQALKLNPHNLRALYGLYLSCNFLANSKTVSSKRKESHKLAQWALEQVSSHTAKQSSIKNNEKLILSLEAALGNMEIKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQG0</accession>
    <name>A0A6I8UQG0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-dehydrosphinganine reductase</fullName>
            <ecNumber evidence="11">1.1.1.102</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Kdsr</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the reduction of 3'-oxosphinganine (3-ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>sphinganine + NADP(+) = 3-oxosphinganine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.102"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:22642"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.102" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001359295.2">
        <property type="nucleotide sequence ID" value="XM_001359258.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQG0"/>
    <dbReference type="FunCoup" id="A0A6I8UQG0">
        <property type="interactions" value="1024"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802366"/>
    <dbReference type="InParanoid" id="A0A6I8UQG0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08939">
        <property type="entry name" value="KDSR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000165">
        <property type="entry name" value="3-ketodihydrosphingosine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045022">
        <property type="entry name" value="KDSR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001359295.2"/>
        </source>
    </evidence>
    <sequence length="354" mass="38812" checksum="07C68C51E308F730" modified="2020-08-12" version="1">MINAIISKFVGGFEWFRLVIMEFSWDIIVSLGLVVLAHSIVYVFVMKKRAKSIDGRHVVVTGGSSGIGVCLAIECVTKGAHVTIIARDEKRLKAAVDQLELVRQRADQKIQYRSLDIGGDYEEVARVLAETEETVGPIYALLNCAGMAICGVFEEVSVRDVHKLMNVNFFGSYNCTRHVLPKMKRAREGIIVITSSQAAMFGIYGYGPYAASKYALRAMAETIAMESREHGVSVTLALPCDTNTPGFEEEEKSKPRETKIISGGGGLLKPEDMAKAILQDALKGNFISTVGAESWLITMLGGGLLPWDGFFTNLLHALVIGPLRLVGYGLHKYFNSIIRKCAKEDRAGDKVESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQR9</accession>
    <name>A0A6I8UQR9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4802393</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359320.2">
        <property type="nucleotide sequence ID" value="XM_001359283.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQR9"/>
    <dbReference type="GeneID" id="4802393"/>
    <dbReference type="KEGG" id="dpo:4802393"/>
    <dbReference type="InParanoid" id="A0A6I8UQR9"/>
    <dbReference type="OMA" id="MNFCQFL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073339">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040365">
        <property type="entry name" value="EFHD1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13025">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13025:SF6">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="207"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="337"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359320.2"/>
        </source>
    </evidence>
    <sequence length="367" mass="40576" checksum="3DF6F6BB4E40597C" modified="2020-08-12" version="1">MSSDADMTDGDYVVSMSVSVSQTTLKNAPNESGEQTVVPPESNLSSKASTVLPQDSAQPGEQSDEPEDSTEESFGDIEREASGISLNEWGKESEENMTTASMSCSSFSSCESYHTIRKMILRRDPHTSNMSLDSGSSLPQINSCDQLMHQRECRVVGGKDSSHDSMLVVMEPKPNFTQHSCMDISVKEGDIGMEEVYHKFVAWFKRPEIDVAFKVFNEVDQDADGYIALTDLKRGLEKLSVPQTHREAKSIMAQVAGPRCSAVHFSHFLLIYASLLNRLQLDKWPTRDAETDRFSRVDEVDVSQVGVHGARRYFEAKISLEGERLKPLSLMCTQPVARPSSLSSVATSRDMGDGTASGLKKLNEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URA4</accession>
    <name>A0A6I8URA4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Serine/arginine-rich splicing factor 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SF2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359460.2">
        <property type="nucleotide sequence ID" value="XM_001359423.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URA4"/>
    <dbReference type="SMR" id="A0A6I8URA4"/>
    <dbReference type="FunCoup" id="A0A6I8URA4">
        <property type="interactions" value="2442"/>
    </dbReference>
    <dbReference type="GeneID" id="4802563"/>
    <dbReference type="KEGG" id="dpo:4802563"/>
    <dbReference type="InParanoid" id="A0A6I8URA4"/>
    <dbReference type="OMA" id="CFADAFK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080004">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12597">
        <property type="entry name" value="RRM1_SRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12601">
        <property type="entry name" value="RRM2_SRSF1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000053">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000507">
        <property type="entry name" value="serine/arginine-rich splicing factor 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034520">
        <property type="entry name" value="SRSF1_RRM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="7"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="115"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="185"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001359460.2"/>
        </source>
    </evidence>
    <sequence length="263" mass="28975" checksum="9EF396EADE432AE3" modified="2020-08-12" version="1">MGSRNECRIYVGNLPPDIRTKDIQDLFHKFGKVTFVDLKNRRGPPFAFVEFEDARDADDAVKARDGYDYDGYRLRVEFPRGGGPGSYRGNRNDRNNGRDGGRMGGRGPPAKRSQYRVMVTGLPPSGSWQDLKDHMREAGDVCFADTYKDGSGVVEFLRHEDMKYAIKKLDDSRFRSHEGEVAYIRVREDSGENDRSGGSGGGGGGAGGGGGGGGGGSSGGGGRDYRDRSRSRSFSSRPRRRGTPTYSPVRRQSYSRSRSRSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URL1</accession>
    <name>A0A6I8URL1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Cks85A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CKS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359811.1">
        <property type="nucleotide sequence ID" value="XM_001359774.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URL1"/>
    <dbReference type="FunCoup" id="A0A6I8URL1">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="GeneID" id="4803006"/>
    <dbReference type="KEGG" id="dpo:4803006"/>
    <dbReference type="InParanoid" id="A0A6I8URL1"/>
    <dbReference type="OMA" id="RPINYGQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082021">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8URL1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000001">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359811.1"/>
        </source>
    </evidence>
    <sequence length="91" mass="10602" checksum="DE724D71D586D79A" modified="2020-08-12" version="1">MPADQIQYSEKYFDDKFEYRHVILPSDLAKHVPKAHLMTETEWRNLGVQQSPGWVHYMMHAPEPHVILFRRKRVGEENAPQVSSQQAAACV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URT7</accession>
    <name>A0A6I8URT7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="2 7">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SelR</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="2 7"/>
    <dbReference type="RefSeq" id="XP_001359909.5">
        <property type="nucleotide sequence ID" value="XM_001359872.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URT7"/>
    <dbReference type="FunCoup" id="A0A6I8URT7">
        <property type="interactions" value="670"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803112"/>
    <dbReference type="InParanoid" id="A0A6I8URT7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8URT7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000004">
        <property type="entry name" value="Peptide-methionine (R)-S-oxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173:SF52">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="9">
        <location>
            <begin position="77"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359909.5"/>
        </source>
    </evidence>
    <sequence length="234" mass="25781" checksum="894595BEB9843FAD" modified="2020-08-12" version="1">MFSLSRHALQHTRNFALIAAAAAKWRHPNRNPLGVLAAAVPIAHCFADTRHDSDEAKTNPSKANNMENKTEKVTVNKEELRKRLTPLQYQVTQEAGTERPYTGCYNKHYEKGVYQCIVCHQDLFSSDTKYDSGCGWPAFNDVLDKGKVTLHRDASIPGGNILLLIAHPERIRTEVRCARCNAHMGHVFEDGPKPTRKRYCINSASIEFVTADPAATGGAAATVATVATVPIAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USK5</accession>
    <name>A0A6I8USK5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Alg9</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001359997.2">
        <property type="nucleotide sequence ID" value="XM_001359960.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USK5"/>
    <dbReference type="FunCoup" id="A0A6I8USK5">
        <property type="interactions" value="2599"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803221"/>
    <dbReference type="InParanoid" id="A0A6I8USK5"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF2">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE ALG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="226"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359997.2"/>
        </source>
    </evidence>
    <sequence length="623" mass="72071" checksum="E79CBDD84B08F12F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT87</accession>
    <name>A0A6I8UT87_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein Obp50d</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Obp50d</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360533.3">
        <property type="nucleotide sequence ID" value="XM_001360496.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT87"/>
    <dbReference type="FunCoup" id="A0A6I8UT87">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803886"/>
    <dbReference type="InParanoid" id="A0A6I8UT87"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052295">
        <property type="entry name" value="Odorant-binding_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066:SF15">
        <property type="entry name" value="GH25962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21066">
        <property type="entry name" value="ODORANT-BINDING PROTEIN 59A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360533.3"/>
        </source>
    </evidence>
    <sequence length="198" mass="22248" checksum="1316D08AE27E14F9" modified="2020-08-12" version="1">MPFGHPVGIVYMGYLVLSGYQKVPEMPHQLALILISFLVSPGAGEPDCSQRPDLNALKNCCKMPLLELSKYTSQCGQYLVNGAHITPCSFECLFEAAKALNGTSLVMENIRKMMETLLESHQEFVDIYTEGFHHCSGEEQAMIKSLKRRRMPVTGKCSSMSVMYGLCAHRYVYRNCPESASRKSTMCIEAREYSIHCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTR6</accession>
    <name>A0A6I8UTR6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein igl isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">igl</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360644.1">
        <property type="nucleotide sequence ID" value="XM_001360607.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTR6"/>
    <dbReference type="FunCoup" id="A0A6I8UTR6">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="GeneID" id="4804014"/>
    <dbReference type="KEGG" id="dpo:4804014"/>
    <dbReference type="InParanoid" id="A0A6I8UTR6"/>
    <dbReference type="OMA" id="NHTNHGK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074901">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000049">
        <property type="entry name" value="Igloo, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10699:SF11">
        <property type="entry name" value="IGLOO, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10699">
        <property type="entry name" value="NEUROMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360644.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="25315" checksum="C8B21027FA8604AA" modified="2020-08-12" version="1">MGCNTSQELKTKDGAAIDAVSNGEPEPSAPQMESEGLQSSAKSSNNHTNHAKSNSITSNGDAKAPNVGGGGGGGASSEATNGIDRPFDKAAITELDDDEDEAKAATKIQAVFRGHKVRETMKKSETKTVTNNGAAASAAGVEEAAGSSAEQTKAELEAEFDPNDKELCHAALKIQSTFRGHLARKLVNKDAPEDEDIQEITKKVAEELDIDLTDPELNKAATKIQASFRGHKTRRDTQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU41</accession>
    <name>A0A6I8UU41_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Centrosomin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cnn</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360950.3">
        <property type="nucleotide sequence ID" value="XM_001360913.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU41"/>
    <dbReference type="FunCoup" id="A0A6I8UU41">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="GeneID" id="4804379"/>
    <dbReference type="KEGG" id="dpo:4804379"/>
    <dbReference type="InParanoid" id="A0A6I8UU41"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078466">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UU41">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090063">
        <property type="term" value="P:positive regulation of microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903358">
        <property type="term" value="P:regulation of Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012943">
        <property type="entry name" value="Cnn_1N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052593">
        <property type="entry name" value="MT-associated_AKAP9-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46501:SF10">
        <property type="entry name" value="CENTROSOMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46501">
        <property type="entry name" value="MYOMEGALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07989">
        <property type="entry name" value="Cnn_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosomin N-terminal motif 1" evidence="5">
        <location>
            <begin position="99"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="340"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="658"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="991"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1070"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="392"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="811"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="363"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="658"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="678"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="694"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="719"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="991"/>
            <end position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360950.3"/>
        </source>
    </evidence>
    <sequence length="1168" mass="132273" checksum="4C2045D2454DA104" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU51</accession>
    <name>A0A6I8UU51_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein link</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">link</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360967.3">
        <property type="nucleotide sequence ID" value="XM_001360930.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU51"/>
    <dbReference type="FunCoup" id="A0A6I8UU51">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804397"/>
    <dbReference type="InParanoid" id="A0A6I8UU51"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026236022" evidence="2">
        <location>
            <begin position="21"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360967.3"/>
        </source>
    </evidence>
    <sequence length="388" mass="43151" checksum="631920129ABDD30A" modified="2020-08-12" version="1">MFGLLVRFLWPLALLQCINARVTLMPKFYGQDSAVIVDAERKVPSKLDSKLQNALYYNFPVYKLIKPGVQTTTTTTAAPVPTNSEYPSDLLEIAQTKLGLKVLPSISELGQLIGTRSNSETIEYVRSLTSNEQGIALMKDYLETLDYELSEDVPDSVDDGDDASDDDNTMDNADVEADFDERPRLETTSRSTITTKKPEMQGSLLQRFGDFMKQYSLWSETTTTAAPATRKPMPSAVSFKPVFVAPPGAAHMRPLLVRQPLPYHYPIPLRPVMIPAKPTQVPSTTAAPRPNKVPHLNTPKHIDVEMQSPPVATHVRQLAEMANISPQVLDGFLKQQPKLAELAKRVSTLSLSQDQTRAMDSQIFMAVKRALSQNEELKRLLESAQTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUY6</accession>
    <name>A0A6I8UUY6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein BomBc2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BomBc2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bomanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361303.1">
        <property type="nucleotide sequence ID" value="XM_001361266.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUY6"/>
    <dbReference type="FunCoup" id="A0A6I8UUY6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="4804798"/>
    <dbReference type="KEGG" id="dpo:4804798"/>
    <dbReference type="InParanoid" id="A0A6I8UUY6"/>
    <dbReference type="OMA" id="CVGCNIN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073504">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013172">
        <property type="entry name" value="Bomanin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08194">
        <property type="entry name" value="DIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026320385" evidence="9">
        <location>
            <begin position="21"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001361303.1"/>
        </source>
    </evidence>
    <sequence length="145" mass="15582" checksum="562B07A3DD1BF647" modified="2020-08-12" version="1">MKYLTLLAVLLLATLAFVQAGKVTINGECKDCNDGRTTSTTHKTPPRGQGRQTTAKPKPKTAPGRRHQASDEDDDDSAGDWTLHQSAGGSQHIGRRHRRQWEPRGQYINLGGAGGGSGRVTTIDSRGYPGTVVRNDDCVGCNIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV52</accession>
    <name>A0A6I8UV52_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4804779</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361288.1">
        <property type="nucleotide sequence ID" value="XM_001361251.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV52"/>
    <dbReference type="GeneID" id="4804779"/>
    <dbReference type="KEGG" id="dpo:4804779"/>
    <dbReference type="InParanoid" id="A0A6I8UV52"/>
    <dbReference type="OMA" id="PWNCSAH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070696">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006616">
        <property type="entry name" value="DM9_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31649">
        <property type="entry name" value="AGAP009604-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31649:SF10">
        <property type="entry name" value="IP19903P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11901">
        <property type="entry name" value="DM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00696">
        <property type="entry name" value="DM9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="18"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001361288.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="28075" checksum="AAF8BD296AC3A3F2" modified="2020-08-12" version="1">MEGETAPTESSPSLNVLCGICNEFYRANDIIFSTASCGHVFHRECLTRWLGRSSTCPQCRATCHRNRIHRIYLNFAERTELDDQEPPKQPVQWVPMDLDINSSRDASNAPEGAIQCGTDEDGLPTYVARGYFNDDLLPASYAPQKKAAFGSWSCRSYRLIEGVEVLVLTDCDHEWVPGSSGSYPPNALPTGYSEIGEVTYTGLGVYEGIKRLGKVHPSHKVMYIPHRGQEVNTSSYEVLVVTPRVEVESPSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW41</accession>
    <name>A0A6I8UW41_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">U7 snRNA-associated Sm-like protein LSm10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Lsm10</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361732.3">
        <property type="nucleotide sequence ID" value="XM_001361695.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW41"/>
    <dbReference type="FunCoup" id="A0A6I8UW41">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805312"/>
    <dbReference type="InParanoid" id="A0A6I8UW41"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071254">
        <property type="term" value="C:cytoplasmic U snRNP body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071208">
        <property type="term" value="F:histone pre-mRNA DCP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071209">
        <property type="term" value="F:U7 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01733">
        <property type="entry name" value="LSm10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052840">
        <property type="entry name" value="U7_snRNA_Sm-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21196">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21196:SF1">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361732.3"/>
        </source>
    </evidence>
    <sequence length="147" mass="17082" checksum="3823C3016D2FCBA8" modified="2020-08-12" version="1">MQKFSSQEKYLIANTLNCWPVMLQGHSVLMDLRNETSVAGVVDIADGHMTCELSNAVLIDRNGGQHPFDNFMVRNRMIRQIHIPTDIDVAQELRQAMEHGVLRRNRVDGRNTKRTFKQKRAEIRHKETLEMIAQQRSEKEQNQSNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWA2</accession>
    <name>A0A6I8UWA2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Prosbeta1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Non-catalytic component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361869.3">
        <property type="nucleotide sequence ID" value="XM_001361832.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWA2"/>
    <dbReference type="FunCoup" id="A0A6I8UWA2">
        <property type="interactions" value="1047"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805474"/>
    <dbReference type="InParanoid" id="A0A6I8UWA2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000010">
        <property type="entry name" value="Proteasome subunit beta type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 12 14" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001361869.3"/>
        </source>
    </evidence>
    <sequence length="225" mass="24386" checksum="0A23A96178054A5F" modified="2020-08-12" version="1">MVYEMDFTDTPVSTGTTIMAVEFDGGVVIGADSRTSSGPYVANRVTDKLTRITDKIYCCRSGSAADTQAIADIVAYSLNYHENQTSKDALVFEAASEFRNYCYNYRDSLLAGIIVAGWDEQRGGQVFSIPLGGMLTREPCTIGGSGSSFIYGYVREHFHEGMSKEECVKFVKTAVQHAIYHDGSSGGVVRVGIITKEGVERRLFYNTESGESEISGTGSSAILAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWA8</accession>
    <name>A0A6I8UWA8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heat shock 70 kDa protein cognate 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Hsc70-5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361878.1">
        <property type="nucleotide sequence ID" value="XM_001361841.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWA8"/>
    <dbReference type="FunCoup" id="A0A6I8UWA8">
        <property type="interactions" value="1585"/>
    </dbReference>
    <dbReference type="GeneID" id="4805483"/>
    <dbReference type="KEGG" id="dpo:4805483"/>
    <dbReference type="InParanoid" id="A0A6I8UWA8"/>
    <dbReference type="OMA" id="MGTDWKI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081138">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11733">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000020">
        <property type="entry name" value="Chaperone protein HscA homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000011">
        <property type="entry name" value="stress-70 protein, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="656"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="662"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="681"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361878.1"/>
        </source>
    </evidence>
    <sequence length="690" mass="74433" checksum="7EDB378D7C302151" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWB5</accession>
    <name>A0A6I8UWB5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SR-related and CTD-associated factor 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Isha</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361927.3">
        <property type="nucleotide sequence ID" value="XM_001361890.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UWB5">
        <property type="interactions" value="2314"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805540"/>
    <dbReference type="InParanoid" id="A0A6I8UWB5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078076">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UWB5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16983">
        <property type="entry name" value="CID_SCAF8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12227">
        <property type="entry name" value="RRM_SCAF4_SCAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000004">
        <property type="entry name" value="splicing factor, arginine/serine-rich 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000576">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF4">
        <property type="entry name" value="PROTEIN CBR-NRD-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CID" evidence="5">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="511"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="149"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="397"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="769"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="848"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="881"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="916"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1256"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="415"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="649"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="769"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="800"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="818"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="857"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="883"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="946"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="967"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1012"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1095"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1154"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1164"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1176"/>
            <end position="1191"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1200"/>
            <end position="1222"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1293"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1307"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1338"/>
            <end position="1353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361927.3"/>
        </source>
    </evidence>
    <sequence length="1360" mass="146539" checksum="6C38D78F26857DCF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWI7</accession>
    <name>A0A6I8UWI7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4805662</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001362024.3">
        <property type="nucleotide sequence ID" value="XM_001361987.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWI7"/>
    <dbReference type="FunCoup" id="A0A6I8UWI7">
        <property type="interactions" value="1331"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805662"/>
    <dbReference type="InParanoid" id="A0A6I8UWI7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080967">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="177"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001362024.3"/>
        </source>
    </evidence>
    <sequence length="348" mass="39796" checksum="499B413A7AD5DED7" modified="2020-08-12" version="1">MNDFMNHLNGLLVFCSRSRVNGGYGQLIASATLFVVNSLWSAVAPCYLGIDESDVSFAYQKTMNNYSYSALASVDHHNRCCGNRAWCVKDICGIVCVIMTWLLILFAEFVVMRLILMPSDYTVFSTINMIIFQALAFLAFASHIRTMLSDPGAVPRGNATKEMIEQMGYREGQMFYKCPKCCSIKPERAHHCSVCQRCIRKMDHHCPWVNNCVGENNQKYFVLFTFYIASISLHTLFLVLTQFAECVKNDWRTCSPYSPPSTIFLLLFLTFEGLMFGIFTIIMLATQLTAIFNDQTGIEQLKKEEARWAKKSRLKSIQSVFGRFSLAWFSPFTEPSCRTKFHTHFYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYC8</accession>
    <name>A0A6I8UYC8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Vacuolar protein sorting-associated protein 41 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">lt</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport pathways.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endosome membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Late endosome membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Early endosome membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Lysosome membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle</location>
            <location evidence="7">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the VPS41 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133261.3">
        <property type="nucleotide sequence ID" value="XM_002133225.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYC8"/>
    <dbReference type="FunCoup" id="A0A6I8UYC8">
        <property type="interactions" value="814"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902747"/>
    <dbReference type="InParanoid" id="A0A6I8UYC8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001204">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 41 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016902">
        <property type="entry name" value="Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057779">
        <property type="entry name" value="Znf_RING_Vps41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23555">
        <property type="entry name" value="zf-RING_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028921">
        <property type="entry name" value="VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="10">
        <location>
            <begin position="27"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="8">
        <location>
            <begin position="560"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Vps41 C-terminal RING finger" evidence="11">
        <location>
            <begin position="790"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR028921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002133261.3"/>
        </source>
    </evidence>
    <sequence length="845" mass="97047" checksum="A36934DD58FF3B4B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ18</accession>
    <name>A0A6I8UZ18_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">C-type lectin 37Db-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133245.3">
        <property type="nucleotide sequence ID" value="XM_002133209.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ18"/>
    <dbReference type="KEGG" id="dpo:6902731"/>
    <dbReference type="InParanoid" id="A0A6I8UZ18"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051004">
        <property type="entry name" value="DC-SIGN_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22802:SF458">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22802">
        <property type="entry name" value="C-TYPE LECTIN SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026078090" evidence="1">
        <location>
            <begin position="25"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="34"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133245.3"/>
        </source>
    </evidence>
    <sequence length="162" mass="18748" checksum="2348B62CC564FB14" modified="2020-08-12" version="1">MKLFASALGLLLSLAAMSLSRVEGFNCTLPLTRVGEKHYFVESKDKLTWYDANERCIRSGLQLATVDSEYKFTELTDFLTSMGYRADWIWIGGFGRQGSWLQFGPARPLPYAKWYISQPDNLNSENCMNLYRIGEPWFMNDYSCRVKLSYICELDSTRVYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZS4</accession>
    <name>A0A6I8UZS4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Post-GPI attachment to proteins factor 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6901618</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134288.2">
        <property type="nucleotide sequence ID" value="XM_002134252.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZS4"/>
    <dbReference type="FunCoup" id="A0A6I8UZS4">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901618"/>
    <dbReference type="InParanoid" id="A0A6I8UZS4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039545">
        <property type="entry name" value="PGAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12892">
        <property type="entry name" value="FGF RECEPTOR ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12892:SF17">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="3">
        <location>
            <begin position="99"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002134288.2"/>
        </source>
    </evidence>
    <sequence length="349" mass="38784" checksum="2234D63E414EA3E8" modified="2020-08-12" version="1">MQNIQNSTAVTSSSSGGTRTGCGAVLSSAADSSGAIPEAKSRKISDGGGGSGSVICSGSGGSSGDSCSLHDPSTAATKDAQPQQQQQQQPMAIHYLASFHEICIVTALLPLVTLFTCFVTAYVYQYDDVHETHCRVYNIIPSISAITGVSPQRYFWRFSIALHIGPRIPIAFVYKNYYRSQLKRISPELVPQASWLINLILVLNCIEIASLGGVTYISNRENYPVHERIFITFMICSLCYMLATIKLNGILNAGQSLNVQQQQSIKWKKFFFVASILSTIGLLIFFAKHRFYCHDLAFSWFAFFEYLIAIANMLYHFTIIWDFPSQYMVIVQGPLENISQYLSNRPKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZY8</accession>
    <name>A0A6I8UZY8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6901175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133876.2">
        <property type="nucleotide sequence ID" value="XM_002133840.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZY8"/>
    <dbReference type="KEGG" id="dpo:6901175"/>
    <dbReference type="InParanoid" id="A0A6I8UZY8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZY8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026026894" evidence="1">
        <location>
            <begin position="20"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133876.2"/>
        </source>
    </evidence>
    <sequence length="122" mass="13380" checksum="FACD18ED622C8C73" modified="2020-08-12" version="1">MRWQLSILLLAAGLSVGAAYRLRRQVIAPTWLRDPFVLPALNASKVVVTPQNLRETAAIRTTIQKAVAEDTYVVAASPPPELQTPVGSIWPMPVPFAPTPLSQPWNLALPIWGIFSLWPTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0U0</accession>
    <name>A0A6I8V0U0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative alpha-L-fucosidase</fullName>
            <ecNumber evidence="3">3.2.1.51</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha-L-fucoside fucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Fuca</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 29 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.51" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002135114.3">
        <property type="nucleotide sequence ID" value="XM_002135078.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0U0"/>
    <dbReference type="FunCoup" id="A0A6I8V0U0">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900755"/>
    <dbReference type="InParanoid" id="A0A6I8V0U0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0U0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004560">
        <property type="term" value="F:alpha-L-fucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000027">
        <property type="entry name" value="Alpha-L-fucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000111">
        <property type="entry name" value="GM24746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016286">
        <property type="entry name" value="FUC_metazoa-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031919">
        <property type="entry name" value="Fucosidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000933">
        <property type="entry name" value="Glyco_hydro_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057739">
        <property type="entry name" value="Glyco_hydro_29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030:SF37">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01120">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16757">
        <property type="entry name" value="Fucosidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001092">
        <property type="entry name" value="Alpha-L-fucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00741">
        <property type="entry name" value="GLHYDRLASE29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00812">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026415866" description="Putative alpha-L-fucosidase" evidence="10">
        <location>
            <begin position="28"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 29 N-terminal" evidence="11">
        <location>
            <begin position="33"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-fucosidase C-terminal" evidence="12">
        <location>
            <begin position="379"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002135114.3"/>
        </source>
    </evidence>
    <sequence length="498" mass="57884" checksum="75F67F7F493D6B39" modified="2020-08-12" version="1">MGLVTRFGFPVWMVVVGLGLVSWRSDAAQGMAPRTRYEPNWASLDQRPLPRWYDEAKVGIFLHYGVYSVPSFGSEWFWHNWLNLRTPEYIQFMQRNYKPDFTYQEFADEFTAELFNATQWALLFKDSGARYVVLTSKHHDGFTLWPSKHSFSWNSMDVGPKRDIVKELAAAVRGVSDLKFGLYYSLFEWFNRLWIDDKLHLLMQQHFVDQKVRPEQLDLVQEYLPEIIWSDGDWEAPAKYWKSEQFIAWLYNDSPVRDTVVTNDRWGMGTACKHGDFYNCADRFNPGVLQSHKWENAFTLERSSWGQRFDVSLSNFMSTKDLIKEIVTTVSCNGNVLINVGPTKYGTILPIFEERLRDMGRWLKINGEGIYGSVPWIYQNDTETPNVWYTRGQQAESNSSIAIYAFVLDYPYDTNELDIHPLGKEVTISRNVHLTGLDFGTNGEVLTKQTTKVVMLGMGNTKVQWTSSHNKLHVKFPPKNHMDKRGLDYAWTLKITIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2G2</accession>
    <name>A0A6I8V2G2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137230.2">
        <property type="nucleotide sequence ID" value="XM_002137194.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2G2"/>
    <dbReference type="KEGG" id="dpo:6897045"/>
    <dbReference type="InParanoid" id="A0A6I8V2G2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137230.2"/>
        </source>
    </evidence>
    <sequence length="322" mass="36591" checksum="89A8AE9749C04B2B" modified="2020-08-12" version="1">MSLSSIKKKEAKGKRKVLPKLRNILANPYKQHSPVLADDEVVQFRQILLEAIKHSEHSVKTFATRHQIHLGLESSLRAINSRRFSCVFVSLSLRPSHIIRLIATSAAVKMPTAPIYAQPKLEELTFEIFGVRALSLVLPPDLAAVSLDLEQWVKKRQKPAPQKIPPPLKKSLKKRQVKVQDLQPEETSQKALEQPKVVAEKQWNDDYISWSSETSCVRIDQIDTQVETQQLGAALSNLAMKAKTKANLEVESKSTIPDDSEENVEHMEVETAEGTIDEDDFLPTDTQRYRPVSVHRIRPNPDKKPKKKRNKKQKQQPLSKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V371</accession>
    <name>A0A6I8V371_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6897503</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Non-catalytic component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137652.2">
        <property type="nucleotide sequence ID" value="XM_002137616.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V371"/>
    <dbReference type="KEGG" id="dpo:6897503"/>
    <dbReference type="InParanoid" id="A0A6I8V371"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V371">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002137652.2"/>
        </source>
    </evidence>
    <sequence length="306" mass="33515" checksum="5CBD8D68BF0C9780" modified="2020-08-12" version="1">MLPAPSQSLFQRSSTGFSFVNRERDNQLAGRKGFIPTPPTTTGSTVVGIKYRDGVIIATDKQGTADCIVTTNCMKKIYCLQDNIYAGGSGIYGDLHQVARLTRAQMDLHRLQMSCKVPVVCANQFVKHLLYDYQGNIRINLIIGGVDNEGPSLYSTRFDGTTEKVPFASNGSGDFAAMTVLEKRWRESLDEKGAESLAIDAVRSGIKNDLYSGSPIHICVIRTDYSVSLYDKIFAGSPVPRPPVWRVKPQPPKVLFAADHALEPIANRLSKSPPESDQVVPKGTRTALALARHKSKKTNNTTEIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V582</accession>
    <name>A0A6I8V582_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Keratin, type I cytoskeletal 9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6896880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736293.3">
        <property type="nucleotide sequence ID" value="XM_003736245.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V582"/>
    <dbReference type="KEGG" id="dpo:6896880"/>
    <dbReference type="InParanoid" id="A0A6I8V582"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248156">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V582">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="767"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="259"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="327"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="434"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="634"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="767"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="795"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="979"/>
            <end position="998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_003736293.3"/>
        </source>
    </evidence>
    <sequence length="1222" mass="129955" checksum="10FA606FCE320BD9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V584</accession>
    <name>A0A6I8V584_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATPase protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ATPase subunit c</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17 18 19" type="primary">ATPsynC</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">MV-25-SWS-2005</strain>
            <strain evidence="16">MV2-25</strain>
            <tissue evidence="18 19">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <strain evidence="16">MV2-25</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736193.1">
        <property type="nucleotide sequence ID" value="XM_003736145.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003736194.1">
        <property type="nucleotide sequence ID" value="XM_003736146.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003736196.1">
        <property type="nucleotide sequence ID" value="XM_003736148.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V584"/>
    <dbReference type="GeneID" id="4800349"/>
    <dbReference type="KEGG" id="dpo:4800349"/>
    <dbReference type="OMA" id="CSRFIAP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074545">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18182">
        <property type="entry name" value="ATP-synt_Fo_c_ATP5G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.20.10:FF:000003">
        <property type="entry name" value="Atp synthase f complex subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="ATP SYNTHASE LIPID-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031:SF0">
        <property type="entry name" value="ATPASE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="15">
        <location>
            <begin position="72"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_003736193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_003736194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_003736196.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="14395" checksum="BD9E177DCC499D12" modified="2020-08-12" version="1">MFVSTVSRIAPVARSALLANSKQYLRPLSSAVISQSQTLAAQNTTPVALLPQIRSFQTSPVTRDIDSAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARNPSLKQQLFSYAILGFALSEAMGLFCLMMAFLLLFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAH7</accession>
    <name>A0A6I8VAH7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase theta</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PolQ</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 21">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="RefSeq" id="XP_015037196.2">
        <property type="nucleotide sequence ID" value="XM_015181710.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VAH7">
        <property type="interactions" value="889"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VAH7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VAH7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:double-strand break repair via alternative nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd18026">
        <property type="entry name" value="DEXHc_POLQ-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA_pol_A_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA polymerase I, putative"/>
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        <property type="entry name" value="DNA polymerase theta"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.300:FF:000813">
        <property type="entry name" value="helicase POLQ-like isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Taq DNA Polymerase, Chain T, domain 4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="DNA_polymerase_A"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
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        <property type="entry name" value="Helicase_C-like"/>
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        <property type="entry name" value="HTH_61"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="POLQ-like_helical"/>
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        <property type="entry name" value="RNaseH_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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        <property type="entry name" value="DNA POLYMERASE I"/>
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        <property type="entry name" value="DNA POLYMERASE THETA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sec63 N-terminal domain-like"/>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="218"/>
            <end position="391"/>
        </location>
    </feature>
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        <location>
            <begin position="438"/>
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    </feature>
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        <location>
            <begin position="1025"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
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            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1178"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1273"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1322"/>
            <end position="1357"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1402"/>
            <end position="1477"/>
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        <location>
            <begin position="1076"/>
            <end position="1087"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1108"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1181"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1273"/>
            <end position="1283"/>
        </location>
    </feature>
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        <location>
            <begin position="1345"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1403"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1430"/>
            <end position="1441"/>
        </location>
    </feature>
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        <location>
            <begin position="1448"/>
            <end position="1462"/>
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        <location>
            <begin position="1463"/>
            <end position="1477"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00074669"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015037196.2"/>
        </source>
    </evidence>
    <sequence length="2287" mass="255275" checksum="0F9B758F370C1F10" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBY0</accession>
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        <submittedName>
            <fullName evidence="7 8">Rho GTPase-activating protein 100F isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="7 8" type="primary">RhoGAP100F</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <strain evidence="6">MV2-25</strain>
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        </source>
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    <sequence length="1880" mass="199649" checksum="2CEA9720712D9C9E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VEM9</accession>
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        <submittedName>
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        <name evidence="14" type="primary">zip</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="14">MV-25-SWS-2005</strain>
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        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
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        <location>
            <begin position="1985"/>
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            <begin position="2005"/>
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            <begin position="179"/>
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            <name>ATP</name>
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    <evidence type="ECO:0000256" key="1">
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        <source>
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        </source>
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    <evidence type="ECO:0000256" key="3">
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015039186.1"/>
        </source>
    </evidence>
    <sequence length="2014" mass="231744" checksum="D89F1CEFA20DD99A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VG41</accession>
    <name>A0A6I8VG41_DROPS</name>
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        <submittedName>
            <fullName evidence="12">Zinc finger protein 189 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cg</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
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    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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    <dbReference type="RefSeq" id="XP_015040574.1">
        <property type="nucleotide sequence ID" value="XM_015185088.2"/>
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        <property type="interactions" value="925"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000217">
        <property type="entry name" value="Combgap, isoform F"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001018">
        <property type="entry name" value="Combgap, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001081">
        <property type="entry name" value="Combgap, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001226">
        <property type="entry name" value="Combgap, isoform F"/>
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        <property type="entry name" value="Combgap, isoform F"/>
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    <dbReference type="FunFam" id="3.30.160.60:FF:001137">
        <property type="entry name" value="Combgap, isoform L"/>
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        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
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        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
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        <property type="match status" value="6"/>
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        <property type="entry name" value="ZnF_C2H2"/>
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    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015040574.1"/>
        </source>
    </evidence>
    <sequence length="844" mass="91494" checksum="162C8D6D80F89A32" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8VG59</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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    <dbReference type="KEGG" id="dpo:26532754"/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="19"/>
            <end position="198"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015040087.1"/>
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    </evidence>
    <sequence length="198" mass="22765" checksum="74DE98865B2E85E5" modified="2020-08-12" version="1">MMIVLLCLLVVFPKGCIPELVLKTTKIECKTNPKFVENVTCYLKAVTWEKSLVQMDCHLKEPLRNPTVHIKLYKKDYNNQYKPFLANVAFNLCDLIAGKAFLPYGLLMWKTLNKFTNANHSCPFVGHLFARDMVFETSNLPSSIPMGAYWISSTFSETYSEGSKASYGTVNWYWEIMRNKRKPKTIKGKRTTLTVVMV</sequence>
</entry>
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    <accession>A0A6I8VL29</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein mwh isoform X1</fullName>
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        <name evidence="5" type="primary">mwh</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <strain evidence="5">MV-25-SWS-2005</strain>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cortical actin cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="FH3_dom"/>
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        <property type="entry name" value="FMNL_animal"/>
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        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF9">
        <property type="entry name" value="MULTIPLE WING HAIRS, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="3">
        <location>
            <begin position="310"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="703"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="883"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1060"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="652"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="781"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1064"/>
            <end position="1081"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015043584.2"/>
        </source>
    </evidence>
    <sequence length="1091" mass="118653" checksum="736B7B264B364310" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNL3</accession>
    <name>A0A6I8VNL3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897852</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232453.1">
        <property type="nucleotide sequence ID" value="XM_033376562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNL3"/>
    <dbReference type="InParanoid" id="A0A6I8VNL3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033232453.1"/>
        </source>
    </evidence>
    <sequence length="195" mass="22258" checksum="A734A912F0FA2EB3" modified="2020-08-12" version="1">MDLSKAERQTPEGNNRHGGAEYLKGLLQDFLCFLIIIFAALLIGWGIFFVLDDSNEWKYGPRPLSSSVMPLQKTSTVAEQPCYRPRQWIQLYIFRSGNSQESDLSPEMAEKPNTDTEQHQSALETKTQLPQVEEQPQVKSPQDDLRVDTDPAINQVESMPSSRLMDLFRRYILGRLAEDNVLDSKPNTIKLDQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNP3</accession>
    <name>A0A6I8VNP3_DROPS</name>
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        <recommendedName>
            <fullName evidence="1">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232488.1">
        <property type="nucleotide sequence ID" value="XM_033376597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNP3"/>
    <dbReference type="KEGG" id="dpo:117183264"/>
    <dbReference type="InParanoid" id="A0A6I8VNP3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="1">
        <location>
            <begin position="60"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232488.1"/>
        </source>
    </evidence>
    <sequence length="172" mass="20017" checksum="49AC3AFE11F96B71" modified="2020-08-12" version="1">MDLMLNKLDGINNLDKKMDIVINKLDRNTAEMVALRSEIGSIKAKVCGEIRKPKVVLPCQPLTTIEELDYFEHNLQEESFLKNVIAELMMSGEKAFEKWIWSSWRSIVSDEVARQCSWRDTEEKKCIRGLRVTLAIRTGFKERFSLEDADFDRITQKFFQYAEDSVEGEKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQ84</accession>
    <name>A0A6I8VQ84_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC13036529</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233038.1">
        <property type="nucleotide sequence ID" value="XM_033377147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQ84"/>
    <dbReference type="KEGG" id="dpo:13036529"/>
    <dbReference type="InParanoid" id="A0A6I8VQ84"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233038.1"/>
        </source>
    </evidence>
    <sequence length="172" mass="20027" checksum="C1B716D276FAF3E7" modified="2020-08-12" version="1">MRSLLICNQPCFQDAVVFKFTNFVCESYNKSFVVFNTCRLKAINRNKVVFNMNATILHPANAITIRCRLFKKANGFKPWMVDRSIDVCRYMRTGYDPYLKIVFGLFHDFTNMNHTCPYVGPQILNGFYLRPEMLRLPLPTGDYLLALHWIFGKNIVDTNASFSFVEDLLKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VRS2</accession>
    <name>A0A6I8VRS2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor eIF2B subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">eIF2B GDP-GTP exchange factor subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">eIF2Bdelta</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233384.1">
        <property type="nucleotide sequence ID" value="XM_033377493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VRS2"/>
    <dbReference type="FunCoup" id="A0A6I8VRS2">
        <property type="interactions" value="2225"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VRS2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070297">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VRS2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140535">
        <property type="term" value="C:intracellular protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000002">
        <property type="entry name" value="Probable translation initiation factor eIF-2B subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233:SF14">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
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    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="129"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
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        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
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            <begin position="81"/>
            <end position="94"/>
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            <begin position="140"/>
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            <begin position="162"/>
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        <location>
            <begin position="209"/>
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            <begin position="222"/>
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            <begin position="277"/>
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            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
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            <dbReference type="ARBA" id="ARBA00043898"/>
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            <dbReference type="ARBA" id="ARBA00044147"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00044356"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033233384.1"/>
        </source>
    </evidence>
    <sequence length="643" mass="69788" checksum="643A35CD7BAEC306" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VRW2</accession>
    <name>A0A6I8VRW2_DROPS</name>
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            <fullName evidence="7 8">Slit homolog 2 protein isoform X1</fullName>
        </submittedName>
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        <name evidence="7 8" type="primary">LOC4804032</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="7 8">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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    </dbReference>
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        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 15-LIKE"/>
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            <dbReference type="RefSeq" id="XP_033233792.1"/>
        </source>
    </evidence>
    <sequence length="502" mass="56035" checksum="3A168125C7E88F0D" modified="2020-08-12" version="1">MFTMIQLKQLQLPFSHAALLLMLAAVVGVGQAGSQNTPQQQVCPEQGDISPCICTVKKNGLDILCETTDLAHITKSMGTLKGKSPIIFYLKLRHNNLPKLQGFVFLALDIRHLTIHNSSLATIEENALSSLGTGLTQLDVSLNQMKTVPSQALQHLFHLLILNLNHNKITVIHNNAFEGLGTLEILTLYENRITQIDPEAFRGLEGKLKRLNLGGNDLSRVPQKSLSILDTLKKLEIQENKIRSIIEGDFEGLVNLDSLILAHNMITTVPANVFIHLTQLNSLELEGNKISVIDKDAFKGLEENLQYLRLGDNQIHAIPSEALRPLHRLRHLDLRNNNINVLADDAFTGFGDSLTFLNLQKNDIKVLPSVLFENLNSLETLNLQNNKLQRIPQDIMEPVIDTLRIIDITDNPLNCSCELTWFPKLLEDLKNKDDEMSQKKKPLCHMSLDNREYFVQAMPTEKMHCAGLNVSPSPTSGGLMRILQVNLLAQIAMCSVAFLISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTH9</accession>
    <name>A0A6I8VTH9_DROPS</name>
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        <submittedName>
            <fullName evidence="6">Mastermind-like protein 2</fullName>
        </submittedName>
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        <name evidence="6" type="primary">LOC4804861</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033234381.1">
        <property type="nucleotide sequence ID" value="XM_033378490.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8VTH9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804861"/>
    <dbReference type="InParanoid" id="A0A6I8VTH9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF109">
        <property type="entry name" value="FI07618P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026075204" evidence="4">
        <location>
            <begin position="21"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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            <end position="221"/>
        </location>
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            <end position="415"/>
        </location>
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            <end position="540"/>
        </location>
    </feature>
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            <end position="590"/>
        </location>
    </feature>
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        <location>
            <begin position="628"/>
            <end position="656"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="767"/>
            <end position="795"/>
        </location>
    </feature>
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            <end position="826"/>
        </location>
    </feature>
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            <begin position="832"/>
            <end position="875"/>
        </location>
    </feature>
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            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
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        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="639"/>
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        </location>
    </feature>
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        <location>
            <begin position="691"/>
            <end position="701"/>
        </location>
    </feature>
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        <location>
            <begin position="770"/>
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        </location>
    </feature>
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        <location>
            <begin position="811"/>
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        </location>
    </feature>
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        <location>
            <begin position="832"/>
            <end position="869"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033234381.1"/>
        </source>
    </evidence>
    <sequence length="1174" mass="131237" checksum="E66ECCFFD2EF0F7C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVX3</accession>
    <name>A0A6I8VVX3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6899580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235226.1">
        <property type="nucleotide sequence ID" value="XM_033379335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VVX3"/>
    <dbReference type="KEGG" id="dpo:6899580"/>
    <dbReference type="InParanoid" id="A0A6I8VVX3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250627">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="3">
        <location>
            <begin position="216"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235226.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="56676" checksum="8A1F1B400D91649F" modified="2020-08-12" version="1">MPEPVNQRHPKKSVGNVTYKGNSFHAKAAVAEHTRCPHCSDSHNLRHCPDLLSKDCFARKAIVDHAKACLNCLSRSHALLKCTSKRNCTQCGQKHHTLLRFPTPAQMATQPHAASHSARSGNSWQYTTSDSSGRATPTQLYARTPLPTQSTAQPLPAVPNTSSGGHPQSAATDSSVRTHSAQLVSATATHRGPSTVLLATALVTIHNPHTGQSAVVRALVDSGSEGTLITEHTVQALNLKRHPISAEIAGVGTTSKNRCTYTTELSLSSCTSQFCTTIDTAFILKTDVIPQILLSDIRRGKENQPIAQHTQLGWIVFGRATSTRSHAVTIRCHHNRLENLVQKFFEIEHLGSAKQLTPEERWCEEHFKRTHIRQRNGKYLVRLPLKRLFDPSQVLGKSRKTSNGKSLNDVLCTGPALQNDLAGVVLNWRFHRFVFAADIQKMYRCIDMNAEDSQYQRIFWHDEHNQPFASYINWRRMNATDIHSRKGSFDTKYTLMTYKAELPLAKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VW03</accession>
    <name>A0A6I8VW03_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mucin-5AC-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234759.1">
        <property type="nucleotide sequence ID" value="XM_033378868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VW03"/>
    <dbReference type="KEGG" id="dpo:6898480"/>
    <dbReference type="InParanoid" id="A0A6I8VW03"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026262226" evidence="2">
        <location>
            <begin position="19"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234759.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="30279" checksum="1D1AEC3347A3E04F" modified="2020-08-12" version="1">MIIQTVGLLFLLAGQVYGQSISIPIPVYSLSSIYSALTGTGGSSNSASSSGSSSSSSSSSNDAQDDYDAYLNEYYSNYWQNFYQNQALYGPYGPYGPYGPNGPYGPSGPFGPTPAPTTAPTRAPTTTPTPAPTSAPITTSTAVAPTATPITTSTAVAPTVNPGTTSTAVAPTVNPGTTSTAVAPSVDPGTSSTTDASTADPGTTSTALPTVNTDMTSDAVGGKRFRTTPLPFHYVPGTFLSPYDLYRINSNPNNMQAAASSSATSAADTSAVYIPYGSVHHRLSRPSPYFGLEANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWV7</accession>
    <name>A0A6I8VWV7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Autotransporter adhesin BpaC-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235438.1">
        <property type="nucleotide sequence ID" value="XM_033379547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWV7"/>
    <dbReference type="KEGG" id="dpo:117183935"/>
    <dbReference type="InParanoid" id="A0A6I8VWV7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="607"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="737"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="764"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="794"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235438.1"/>
        </source>
    </evidence>
    <sequence length="910" mass="98366" checksum="5E69C4CFBCF44E90" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYI1</accession>
    <accession>Q29LB4</accession>
    <name>A0A6I8VYI1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial inner membrane protease ATP23</fullName>
            <ecNumber evidence="6">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">LOC4817679</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10">MV-25-SWS-2005</strain>
            <tissue evidence="9 10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M76 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_033236047.1">
        <property type="nucleotide sequence ID" value="XM_033380156.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236048.1">
        <property type="nucleotide sequence ID" value="XM_033380157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYI1"/>
    <dbReference type="KEGG" id="dpo:4817679"/>
    <dbReference type="OMA" id="EAHQNCV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078681">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019165">
        <property type="entry name" value="Peptidase_M76_ATP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711:SF0">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE ATP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09768">
        <property type="entry name" value="Peptidase_M76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 6 9" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="14"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033236047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033236048.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="32510" checksum="F52B2D541A7F5160" modified="2020-08-12" version="1">MDFLTKVWAKEAPTPIDIETPTDTKAKPPTPPVVAIDSQDVQDANRSNDQTPSNESKDWGYDLYPERRGVTFKPKWTRILFGMEGQENIDRYKCEQNVYWCVKNGPLVKLMMGALKSSGCPIDLRRHISCEVCDPSVTGGYDPKLNQIVVCQNMAKNKSMVHGVLTHEMIHMFDYCNNDLDFRNVDHLACTEIRAANLAHCSFLSAMFQGDASPFNVKEAHQNCVKTKALASVLAVRNISKSDAIAAVERVFPKCYADLEPIGRRIRRNSTDQQKAYMEGPMYGYDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1H0</accession>
    <name>A0A6I8W1H0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial chaperone BCS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">BCS1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 20 21" type="primary">Bcs1</name>
    </gene>
    <organism evidence="18 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19 20 21">MV-25-SWS-2005</strain>
            <tissue evidence="19 20 21">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. BCS1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357167.3">
        <property type="nucleotide sequence ID" value="XM_001357131.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236563.1">
        <property type="nucleotide sequence ID" value="XM_033380672.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236564.1">
        <property type="nucleotide sequence ID" value="XM_033380673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W1H0"/>
    <dbReference type="KEGG" id="dpo:4817725"/>
    <dbReference type="OMA" id="WMTLYQR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078518">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19510">
        <property type="entry name" value="RecA-like_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000768">
        <property type="entry name" value="Probable mitochondrial chaperone bcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057495">
        <property type="entry name" value="AAA_lid_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014851">
        <property type="entry name" value="BCS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050747">
        <property type="entry name" value="Mitochondrial_chaperone_BCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23070">
        <property type="entry name" value="BCS1 AAA-TYPE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25426">
        <property type="entry name" value="AAA_lid_BCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08740">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01024">
        <property type="entry name" value="BCS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="BCS1 N-terminal" evidence="17">
        <location>
            <begin position="23"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="223"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001357167.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033236563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_033236564.1"/>
        </source>
    </evidence>
    <sequence length="431" mass="48671" checksum="B4878D248FFB65AB" modified="2020-08-12" version="1">MAFTELLSSLSTNPYFGAGFGLFGIGAGAAILRKGLQGGIILFRRHLMITLEVPCRDKSYQWLLKWITIKGARKTQHLSVETNFLQNDNGQIKTSYDFIPSIGKHIFKYKGNWIQVERTREQQTLDLHMGVPWESVTLTAFGNNKQIYSDILDEARYLALEETVGKTLLYTAMGAEWRPFGHPRRRRPIGSVVLDSGVSKKIIADCNDFIQSSVWYTQRGIPYRRGYLLYGPPGCGKSSFITALAGELEYSVCLLNLSERGLTDDRLNHLLNVAPEQTIILLEDIDAAFASRETTLQQKSAYEGINRITFSGLLNCLDGVGSTEARIVFMTTNYLDRLDPALIRPGRIDLKEYIGYCTEYQLEEMFKNFFNNTDTDAGVNSVEFAQRVKSFGRPVSPAQIQGFFMKHKLSSPETVINCCKDIWEDSLNINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W505</accession>
    <name>A0A6I8W505_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Protein TANC1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">rols</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15 16">MV-25-SWS-2005</strain>
            <tissue evidence="15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Postsynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TANC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238450.1">
        <property type="nucleotide sequence ID" value="XM_033382559.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238451.1">
        <property type="nucleotide sequence ID" value="XM_033382560.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W505">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000022">
        <property type="entry name" value="protein TANC2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000036">
        <property type="entry name" value="protein TANC2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058018">
        <property type="entry name" value="AAA_lid_TANC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058056">
        <property type="entry name" value="WH_TANC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF55">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25520">
        <property type="entry name" value="AAA_lid_TANC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25521">
        <property type="entry name" value="WHD_TANC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="347"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="1428"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="1539"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="1572"/>
            <end position="1600"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="1606"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="1639"/>
            <end position="1671"/>
        </location>
    </feature>
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            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="122"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="353"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="438"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="390"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="518"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="537"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033241823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033241824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033241825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033241826.1"/>
        </source>
    </evidence>
    <sequence length="631" mass="66365" checksum="24348270FAD3C540" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEV7</accession>
    <name>A0A6I8WEV7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Max-binding protein MNT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myc antagonist MNT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 16 17" type="primary">Mnt</name>
    </gene>
    <organism evidence="14 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15 16 17">MV-25-SWS-2005</strain>
            <tissue evidence="15 16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer with MAX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355022.3">
        <property type="nucleotide sequence ID" value="XM_001354986.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241168.1">
        <property type="nucleotide sequence ID" value="XM_033385277.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241169.1">
        <property type="nucleotide sequence ID" value="XM_033385278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEV7"/>
    <dbReference type="KEGG" id="dpo:4814819"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072244">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WEV7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11402">
        <property type="entry name" value="bHLHzip_Mnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000034">
        <property type="entry name" value="MAX network transcriptional repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969">
        <property type="entry name" value="MAX DIMERIZATION, MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969:SF99">
        <property type="entry name" value="MAX-BINDING PROTEIN MNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="485"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="453"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="708"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="535"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="453"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="708"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="744"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001355022.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033241168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033241169.1"/>
        </source>
    </evidence>
    <sequence length="856" mass="85562" checksum="6E067D0630288747" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WF55</accession>
    <name>A0A6I8WF55_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Neprilysin-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">Nep2</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <strain evidence="13">MV2-25</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015038020.2">
        <property type="nucleotide sequence ID" value="XM_015182534.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241614.1">
        <property type="nucleotide sequence ID" value="XM_033385723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WF55"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF224">
        <property type="entry name" value="NEPRILYSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="12">
        <location>
            <begin position="111"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="11">
        <location>
            <begin position="572"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015038020.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033241614.1"/>
        </source>
    </evidence>
    <sequence length="778" mass="88886" checksum="A07C731FF59B84C0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>B5DHN9</accession>
    <accession>A0A6I8UX23</accession>
    <name>B5DHN9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Male-specific sperm protein Mst87F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6903539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132414.1">
        <property type="nucleotide sequence ID" value="XM_002132378.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DHN9"/>
    <dbReference type="STRING" id="46245.B5DHN9"/>
    <dbReference type="GeneID" id="6903539"/>
    <dbReference type="KEGG" id="dpo:6903539"/>
    <dbReference type="HOGENOM" id="CLU_214593_0_0_1"/>
    <dbReference type="InParanoid" id="B5DHN9"/>
    <dbReference type="OMA" id="GFWHGRC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246828">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DHN9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002132414.1"/>
        </source>
    </evidence>
    <sequence length="52" mass="5380" checksum="9C724C91DE58899F" modified="2008-10-14" version="1">MCSWPCYPSAALCPCGGCGPSGFYDPCFGPYNGPFNSWCGASGPGFWGGRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKA6</accession>
    <accession>A0A6I8UYC0</accession>
    <name>B5DKA6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="3">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6902395</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002133330.1">
        <property type="nucleotide sequence ID" value="XM_002133294.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKA6"/>
    <dbReference type="STRING" id="46245.B5DKA6"/>
    <dbReference type="MEROPS" id="T01.010"/>
    <dbReference type="GeneID" id="6902395"/>
    <dbReference type="KEGG" id="dpo:6902395"/>
    <dbReference type="eggNOG" id="KOG0174">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_5_2_1"/>
    <dbReference type="InParanoid" id="B5DKA6"/>
    <dbReference type="OMA" id="PWAGEVH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249452">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DKA6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002133330.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="26096" checksum="1DE0DE5BD27E3D5F" modified="2008-10-14" version="1">MSNSSWLDLDLINSFTPVECGTTIMAAEFDGGVVLGADSRTTAGGHVANRITDKVTRITDNVYCCRSGSAADTQELSEVVSNLMWHREMHTGEKTLVRDVAAEFRDKCYNGRKYLYAGIIVGGWDDLHGAQVYNISLGGMMMREQLSYGGSGSIYISGMLRDGYRPNMEKEELVLLFRSALKQAIHHDSFSGGVSRIAIITKEGIERRVYYNTSTGKAQATGTDVLLGCGETKKPRKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q295P6</accession>
    <name>ADAL_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Adenosine deaminase-like protein</fullName>
            <ecNumber>3.5.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA11319</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of the free cytosolic methylated adenosine nucleotide N(6)-methyl-AMP (N6-mAMP) to produce inositol monophosphate (IMP) and methylamine. Is required for the catabolism of cytosolic N6-mAMP, which is derived from the degradation of mRNA containing N6-methylated adenine (m6A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>N(6)-methyl-AMP + H2O + H(+) = IMP + methylamine</text>
            <dbReference type="Rhea" id="RHEA:16001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:59338"/>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:16002"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.-"/>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL28662.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q295P6"/>
    <dbReference type="SMR" id="Q295P6"/>
    <dbReference type="FunCoup" id="Q295P6">
        <property type="interactions" value="1257"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q295P6"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0286110">
        <property type="protein sequence ID" value="FBpp0284548"/>
        <property type="gene ID" value="FBgn0071373"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802632"/>
    <dbReference type="eggNOG" id="KOG1097">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039228_3_0_1"/>
    <dbReference type="InParanoid" id="Q295P6"/>
    <dbReference type="OMA" id="RPQFKPY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071373">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062154">
        <property type="term" value="F:N6-methyl-AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00443">
        <property type="entry name" value="ADA_AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000033">
        <property type="entry name" value="Adenosine deaminase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF42">
        <property type="entry name" value="ADENOSINE DEAMINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000285095" description="Adenosine deaminase-like protein">
        <location>
            <begin position="1"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>N(6)-methyl-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>N(6)-methyl-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>N(6)-methyl-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="100"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>N(6)-methyl-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>N(6)-methyl-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>N(6)-methyl-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>N(6)-methyl-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>N(6)-methyl-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="1">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P03958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q6DHV7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q8LPL7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="340" mass="38455" checksum="36085C55097A763B" modified="2009-01-20" version="2">MWKFLKEMPKVELHAHLNGSLNTNSLQDLAEKVYGNTSEEFSHLCARFVNFEKDSNLDKCFEKFAFVHELTSTAAGLQYATELVIRDFANDNIQYLELRTTPKANKNYLRRDYLRIVLDTIKRSRKKYPNILVKLLPSINRSEPVAVAEETVALALEFAKTDPDLVVGIDLSGIPTKGKFTDFCGALDLARREGLKLVIHCAEIDNPPEIKEMLSFGMSRCGHGTYLTEEDFAQMKAANIPIECCLTSNIKSGSVSSFEEHHLKRLMESDAPRVVCTDDSGVFDTSLTNEFLLVVETFNVTRDQCIDLTLEAVKHSFASEQERQQMALKVEHYVNSLQTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E71</accession>
    <accession>A0A6I8UB37</accession>
    <name>Q29E71_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Geranylgeranyl pyrophosphate synthase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">qm</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352692.1">
        <property type="nucleotide sequence ID" value="XM_001352656.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E71"/>
    <dbReference type="SMR" id="Q29E71"/>
    <dbReference type="FunCoup" id="Q29E71">
        <property type="interactions" value="1866"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E71"/>
    <dbReference type="GeneID" id="4812535"/>
    <dbReference type="KEGG" id="dpo:4812535"/>
    <dbReference type="eggNOG" id="KOG0777">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_6_0_1"/>
    <dbReference type="InParanoid" id="Q29E71"/>
    <dbReference type="OMA" id="FYSKAFF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081177">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29E71">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120531">
        <property type="term" value="F:prenyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042811">
        <property type="term" value="P:pheromone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000009">
        <property type="entry name" value="Geranylgeranyl pyrophosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352692.1"/>
        </source>
    </evidence>
    <sequence length="339" mass="38896" checksum="75F8850602577A71" modified="2006-04-04" version="1">MEELNSILQKTKDKSTQKEQDEILLQPFTYIQQIPGKQFRSELALAFNHWLLIPAEKLAQIGDIVQMLHNSSLLIDDIEDNSILRRGVPVAHSIYGVASTINAANYALFLALEKVQLLGHPEATKVYTEQLLELHRGQGMEIYWRDSFTCPSESDYKLMTVRKTGGLFMLAIRLMQLFSANKEDYSKLTAILGLYFQIRDDYCNLSLKEYTENKSFAEDLTEGKFGFPVIHAVRSQKQDKQVLHILRQRTHDIEVKKYCISLLEKLGSFQYTRKVLETLDAEARAEVMRLGSNPYMDRLLNKLLSWKTSDSVSITQSNQINHNNVTARNSPNQNTLNCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EN8</accession>
    <accession>A0A6I8UAR7</accession>
    <name>Q29EN8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine 4-monooxygenase</fullName>
            <ecNumber evidence="5">1.14.16.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phe-4-monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Hn</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-phenylalanine + O2 = (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tyrosine</text>
            <dbReference type="Rhea" id="RHEA:20273"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="EC" id="1.14.16.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 13">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001352525.1">
        <property type="nucleotide sequence ID" value="XM_001352489.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EN8"/>
    <dbReference type="FunCoup" id="Q29EN8">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EN8"/>
    <dbReference type="GeneID" id="4812210"/>
    <dbReference type="KEGG" id="dpo:4812210"/>
    <dbReference type="eggNOG" id="KOG3820">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023198_0_1_1"/>
    <dbReference type="InParanoid" id="Q29EN8"/>
    <dbReference type="OMA" id="FHDEVYR"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00337"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080320">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EN8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004505">
        <property type="term" value="F:phenylalanine 4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042416">
        <property type="term" value="P:dopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04904">
        <property type="entry name" value="ACT_AAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03347">
        <property type="entry name" value="eu_PheOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.800.10:FF:000004">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACT-like_dom_sf"/>
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        <property type="entry name" value="ACT_dom"/>
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        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
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        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
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        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041912">
        <property type="entry name" value="Euk_PheOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005961">
        <property type="entry name" value="Phe-4-hydroxylase_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01268">
        <property type="entry name" value="Phe4hydrox_tetr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF24">
        <property type="entry name" value="PHENYLALANINE-4-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
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    </dbReference>
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        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
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    </dbReference>
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        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
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        <location>
            <begin position="36"/>
            <end position="107"/>
        </location>
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        <location>
            <begin position="105"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="289"/>
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        <ligand>
            <name>Fe cation</name>
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        </ligand>
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        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Fe cation</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001060"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001352525.1"/>
        </source>
    </evidence>
    <sequence length="452" mass="51721" checksum="CE5009EA2E583975" modified="2006-04-04" version="1">MYQRQVSFDKPTRIEDSAYIAEGVDIQEARNTCLLFSPKDSSLSSGALANILAIFKKHDINLVHIESRSSLRVPGYEFFVECDGKSGALGRAIEDVKDQCSYFNIISRDYKDNATAVPWFPRRIRDLDRFANQILSYGAELDSDHPGFTDPEYRKRRKYFADIAYNYKHGETLPHVEYSKEEIETWGIIFRNLSKLYKTHACREYNHVFPLLVDNCGFREDNIPQLEDVSNFLRDCTGFTLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHPSKPMYTPEPDVCHELLGHVPLFADPAFAQFSQEIGLASLGAPDDYIEKLSTIFWFTVEYGLCRQEGELKAFGAGLLSSYGELEYCLTEKPMLKDFEPEVTGITKYPITQFQPLYYVADSFESAKEKTIKFANSIPRPFGVRYNAYTQSVEVLDSKPQISNLMNNINSEFQILQNAIVKLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q29F73</accession>
    <accession>A0A6I8UED7</accession>
    <name>Q29F73_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4813993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354147.1">
        <property type="nucleotide sequence ID" value="XM_001354111.4"/>
    </dbReference>
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    <dbReference type="GeneID" id="4813993"/>
    <dbReference type="KEGG" id="dpo:4813993"/>
    <dbReference type="eggNOG" id="ENOG502S9HT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089073_0_0_1"/>
    <dbReference type="InParanoid" id="Q29F73"/>
    <dbReference type="OMA" id="GQMFGIN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076645">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001354147.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="34670" checksum="C936CE9B4CAA1A08" modified="2006-04-04" version="1">MTSRWIFLILLLYVGAQEGNAGTVLDNVGDSLKMAGQMFGINTAADVANLVAQAFSGNGSRGKPLGLMSILQHGFQPSEDQAEQNQSEQQEEPPQEDDGTRRPPHPPPLMGSFDSGQLLTNMLRMVGFDARKLGALALNAIIMIAQAIGNTLMQATRGGAGGNTGAAFEGPEALYEPNDHQPRSLSMGSPIDWFLQRPGTHSKRLLGRIMDRDLPEHILDMIESKEDLERGHEAGCLKMLMCKSKPIIWGMQNSLKKRLAGEPELLEEQENHNFFNKGIFFKFLPSLKDFKEHSAGCETRFNEECPRNATTQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FU0</accession>
    <accession>A0A6I8UEZ4</accession>
    <name>Q29FU0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Calcitonin gene-related Peptide type 1 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC4814427</name>
        <name evidence="18 19 20" type="synonym">hec</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18 19 20">MV-25-SWS-2005</strain>
            <strain evidence="18">MV2-25</strain>
            <tissue evidence="18 19 20">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354436.3">
        <property type="nucleotide sequence ID" value="XM_001354400.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042285.1">
        <property type="nucleotide sequence ID" value="XM_015186799.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042286.1">
        <property type="nucleotide sequence ID" value="XM_015186800.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FU0"/>
    <dbReference type="STRING" id="46245.Q29FU0"/>
    <dbReference type="GeneID" id="4814427"/>
    <dbReference type="KEGG" id="dpo:4814427"/>
    <dbReference type="eggNOG" id="KOG4564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002753_4_2_1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078162">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FU0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004948">
        <property type="term" value="F:calcitonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15260">
        <property type="entry name" value="7tmB1_NPR_B4_insect-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003287">
        <property type="entry name" value="GPCR_2_calcitonin_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF43">
        <property type="entry name" value="HECTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01350">
        <property type="entry name" value="CTRFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 18 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="328"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="410"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="15">
        <location>
            <begin position="117"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="16">
        <location>
            <begin position="212"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001354436.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015042285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015042286.1"/>
        </source>
    </evidence>
    <sequence length="504" mass="57534" checksum="E6F2E4D0314CB828" modified="2016-01-20" version="3">MTTVSHFETPPMEAMETSASSSPDQAQPQDNLRIFLKHLYAECVYRYQNDTYDEDSTVPATDYEVEVPENFSPVPRYLENVVLNEGSIDMQSVDEASAANNELYATIITATMATNLEEHQRQQQQANLGEKNTTSLFCPLNFDGYLCWPRTPAGTVLSQYCPDFVEGFSSRFLAHKTCLENGSWFRHPVSNLTWSNYTNCVDYEDLEFRQFINELYVKGYTLSLMALLISIIIFLGFKSLRCTRIRIHVHLFASLSCTCVAWILWYRLVVERPEFVAESPLWCIGLHLVVHYFMLVNYFWMFCEGLHLHLVLVVVFVKDTIVMRWFKIISWLSPIHVALVYGAARHYSSTDNENCWINDSLFLWIFSVPITLSLLASFIFLINVLRVIVRKLHPQSAQPAPLAIRKAVRATIILVPLFGLQHFLLPYRPDAGTQLDRFYQLLSVVLVSLQGFVVSFLFCFANHDVTFAMRTLLNKWVPTLVAAPPAGSNTGQLATTTPSRELGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HE8</accession>
    <accession>A0A6I8VHS6</accession>
    <name>Q29HE8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Transmembrane emp24 domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">p24-1</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15">MV-25-SWS-2005</strain>
            <tissue evidence="14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354755.2">
        <property type="nucleotide sequence ID" value="XM_001354719.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041650.1">
        <property type="nucleotide sequence ID" value="XM_015186164.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HE8"/>
    <dbReference type="SMR" id="Q29HE8"/>
    <dbReference type="STRING" id="46245.Q29HE8"/>
    <dbReference type="GeneID" id="4815254"/>
    <dbReference type="KEGG" id="dpo:4815254"/>
    <dbReference type="eggNOG" id="KOG1693">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066963_6_0_1"/>
    <dbReference type="OMA" id="NAEDCFY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075185">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HE8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014308597" evidence="11">
        <location>
            <begin position="31"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="12">
        <location>
            <begin position="42"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001354755.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015041650.1"/>
        </source>
    </evidence>
    <sequence length="221" mass="24697" checksum="20E134CAE891EB7D" modified="2008-10-14" version="2">MMGSTRYTQTADKLVLALLAATALLQCAAGVEFTFDLADNAEDCFYEEIKRNTSAYFEFQVSAGGQLDVDVYLKDPQGKVIYKLERATFDSHQFVAETTGVYTACFGNQFSAFSHKIVYVDFQVGDEPALPGVDEHATVLTQMETSSQAIHKGLNDILDAQTHHRLREAQGRKRAEDLNQRVMVWSSLETAAVVLIGITQIMVLRNFFTDRKPSQAHYGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JM4</accession>
    <accession>A0A6I8WCV9</accession>
    <name>Q29JM4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">28 kDa heat- and acid-stable phosphoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240399.1">
        <property type="nucleotide sequence ID" value="XM_033384508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JM4"/>
    <dbReference type="FunCoup" id="Q29JM4">
        <property type="interactions" value="397"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JM4"/>
    <dbReference type="KEGG" id="dpo:4816022"/>
    <dbReference type="eggNOG" id="KOG3375">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084870_1_0_1"/>
    <dbReference type="InParanoid" id="Q29JM4"/>
    <dbReference type="OMA" id="DRQMRVQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071063">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019380">
        <property type="entry name" value="Casein_kinase_sb_PP28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039876">
        <property type="entry name" value="HAP28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22055">
        <property type="entry name" value="28 KDA HEAT- AND ACID-STABLE PHOSPHOPROTEIN PDGF-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10252">
        <property type="entry name" value="PP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Casein kinase substrate phosphoprotein PP28" evidence="2">
        <location>
            <begin position="112"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033240399.1"/>
        </source>
    </evidence>
    <sequence length="214" mass="23124" checksum="127ACD9E3AD1777B" modified="2006-04-04" version="1">MPRGKFVNHKGRSRHFTSPEELQQESEEESDQSSGSGSGEETGGDKASGSAATSKSKPPATRKTATRPAHRQKQRPAVSSSESDSEEESEDDSEAEARDAKKGVASLIEIENPNRVTKKATQKIATLKLDDGGSSTTSSSAAAKPELSRREREQIEKQKARQRYEKLHAAGKTTEAKADLARLALIRQQREEAAAKREAEKKVAADTGKKPGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JR7</accession>
    <accession>A0A6I8UI42</accession>
    <name>Q29JR7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein quiver</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4816110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355835.2">
        <property type="nucleotide sequence ID" value="XM_001355799.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JR7"/>
    <dbReference type="STRING" id="46245.Q29JR7"/>
    <dbReference type="GeneID" id="4816110"/>
    <dbReference type="KEGG" id="dpo:4816110"/>
    <dbReference type="eggNOG" id="ENOG502TKX1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1715183_0_0_1"/>
    <dbReference type="InParanoid" id="Q29JR7"/>
    <dbReference type="OMA" id="THQLHGC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072095">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JR7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355835.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16283" checksum="B449B8CA9BDCFBD6" modified="2008-10-14" version="2">MYTKTIIASVLIAIICVQFAESLVCYTCTTPNDCKKPKKTTCSNAAANETSHHLAVYHQGVINATSTRFDCMSLQYTWNNQVIHQLHGCLHPAVGPCNLSLKPAYGHYNKTHCRTCSGDKCNKSPAGKVSSSIYTILAGVVGLLLVKTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29ME1</accession>
    <accession>A0A6I8UK65</accession>
    <name>Q29ME1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Male accessory gland serine protease inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4817378</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356687.1">
        <property type="nucleotide sequence ID" value="XM_001356651.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29ME1"/>
    <dbReference type="FunCoup" id="Q29ME1">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29ME1"/>
    <dbReference type="GeneID" id="4817378"/>
    <dbReference type="KEGG" id="dpo:4817378"/>
    <dbReference type="eggNOG" id="KOG4295">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164133_0_2_1"/>
    <dbReference type="InParanoid" id="Q29ME1"/>
    <dbReference type="OMA" id="KPEMCQQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077504">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22634">
        <property type="entry name" value="Kunitz_SCI-I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF374">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356687.1"/>
        </source>
    </evidence>
    <sequence length="89" mass="9331" checksum="5EDF6D89DCEF177B" modified="2006-04-04" version="1">MKYLGIAVIGLLIGSLTSGGLLVEAKPEMCTQPHSKDGMAQDGAACMAFMPAWTYDAKKNACSEFVFGGCGGNANQFSSQVECEKACKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MR4</accession>
    <accession>A0A6I8UJM1</accession>
    <name>Q29MR4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase II subunit RPB11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA-directed RNA polymerase II subunit J</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Polr2J</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356566.1">
        <property type="nucleotide sequence ID" value="XM_001356530.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MR4"/>
    <dbReference type="FunCoup" id="Q29MR4">
        <property type="interactions" value="1001"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MR4"/>
    <dbReference type="GeneID" id="4817442"/>
    <dbReference type="KEGG" id="dpo:4817442"/>
    <dbReference type="eggNOG" id="KOG4392">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_2_2_1"/>
    <dbReference type="InParanoid" id="Q29MR4"/>
    <dbReference type="OMA" id="MNAPSRY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079893">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MR4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06926">
        <property type="entry name" value="RNAP_II_RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037685">
        <property type="entry name" value="RBP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF16">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="10">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356566.1"/>
        </source>
    </evidence>
    <sequence length="117" mass="13399" checksum="1ACA3D43E495D259" modified="2006-04-04" version="1">MNAPPTFESFLLYEGEKKIIKELDTKVTNAAIFTINKEDHTLGNMIRNQLLKDPNVLFAGYKVPHPLEHKFVIRIQTTAEYSPQEAFMNAITDLLAELSLFEERFKDAIKEKKEGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ78</accession>
    <accession>A0A6I8VL61</accession>
    <name>Q2LZ78_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8 9">F-box only protein 32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8 9 10" type="primary">LOC4813178</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8 9">MV-25-SWS-2005</strain>
            <tissue evidence="7 8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353895.1">
        <property type="nucleotide sequence ID" value="XM_001353859.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043277.1">
        <property type="nucleotide sequence ID" value="XM_015187791.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043278.1">
        <property type="nucleotide sequence ID" value="XM_015187792.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043279.1">
        <property type="nucleotide sequence ID" value="XM_015187793.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ78"/>
    <dbReference type="STRING" id="46245.Q2LZ78"/>
    <dbReference type="GeneID" id="4813178"/>
    <dbReference type="KEGG" id="dpo:4813178"/>
    <dbReference type="eggNOG" id="KOG3926">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065667_0_0_1"/>
    <dbReference type="OMA" id="AWDTMAK"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071179">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZ78">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040394">
        <property type="entry name" value="FBX25/32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13123">
        <property type="entry name" value="LD30288P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13123:SF7">
        <property type="entry name" value="LD30288P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015043277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015043278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015043279.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="41004" checksum="5923593296DCB7A6" modified="2006-02-21" version="1">MAFISKDFRSPGEAWVKTDVGWERLKVLECGGKRKRHNSEGSSYQVVASDSQEVEEEGSSMPPHYHITIRCTREIAGFNGLSEAVKRLDFQRSVRDRKRFHYICAFLLLVSNKGIASLPGSAQRQLLQMVEEVASHVNDSQQHPNVLRGLALQLEQIVNQENQKCWGKPLGSTYLWKEHMATIKRIQRVASQIEIREPDPKAKPKLHELPEECVREIILCIADHRDLESAAKAWDTMAKLVSEQRIWRELTRFHFNQRQIHTILDLDKFKQMGEIKDWKQIYHQLRRTYGVNDDYQFAEVLALCRSCCCLFWPSDGHPCIVDQSPDYKQRLEEAGGQQALSQPVPPAQFLSYFSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZB1</accession>
    <name>UBP36_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Ubiquitin carboxyl-terminal hydrolase 36</fullName>
            <ecNumber>3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Deubiquitinating enzyme 36</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein scrawny</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Ubiquitin thioesterase 36</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Ubiquitin-specific-processing protease 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">Usp36</name>
        <name type="synonym">scny</name>
        <name type="ORF">GA18934</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Controls selective autophagy activation by ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with atms/PAF1, but not with CycT.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12"/>
    <dbReference type="EMBL" id="CH379069">
        <property type="protein sequence ID" value="EAL29597.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZB1"/>
    <dbReference type="SMR" id="Q2LZB1"/>
    <dbReference type="FunCoup" id="Q2LZB1">
        <property type="interactions" value="552"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZB1"/>
    <dbReference type="eggNOG" id="KOG1865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006208_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LZB1"/>
    <dbReference type="OMA" id="VCAMAKT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030718">
        <property type="term" value="P:germ-line stem cell population maintenance"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016242">
        <property type="term" value="P:negative regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035019">
        <property type="term" value="P:somatic stem cell population maintenance"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd02661">
        <property type="entry name" value="Peptidase_C19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000085">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0788">Thiol protease</keyword>
    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="chain" id="PRO_0000378500" description="Ubiquitin carboxyl-terminal hydrolase 36">
        <location>
            <begin position="1"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="domain" description="USP">
        <location>
            <begin position="171"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="94"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="505"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="687"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="926"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1012"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="633"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="655"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="729"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="752"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="766"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="779"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="799"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="825"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="842"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="876"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="926"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1048"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3 4">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="438"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine" evidence="1">
        <location>
            <position position="673"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine" evidence="1">
        <location>
            <position position="682"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="692"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="694"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="766"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="801"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine" evidence="1">
        <location>
            <position position="804"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="807"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine" evidence="1">
        <location>
            <position position="846"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine" evidence="1">
        <location>
            <position position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9VRP5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="1059" mass="116372" checksum="A479D84A1DBFE65D" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZP8</accession>
    <accession>A0A6I8W7I9</accession>
    <name>Q2LZP8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Novel acetylcholine receptor chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4813444</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Peroxisome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DoxX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238599.1">
        <property type="nucleotide sequence ID" value="XM_033382708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZP8"/>
    <dbReference type="FunCoup" id="Q2LZP8">
        <property type="interactions" value="264"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813444"/>
    <dbReference type="eggNOG" id="ENOG502RXPR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121618_2_1_1"/>
    <dbReference type="InParanoid" id="Q2LZP8"/>
    <dbReference type="OMA" id="INKEMHR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072672">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZP8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000010">
        <property type="term" value="P:positive regulation of protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040399">
        <property type="entry name" value="TMEM35A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13163:SF0">
        <property type="entry name" value="NOVEL ACETYLCHOLINE RECEPTOR CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13163">
        <property type="entry name" value="SPINAL CORD EXPRESSION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13564">
        <property type="entry name" value="DoxX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033238599.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="17797" checksum="159C61E27FB6E9A4" modified="2008-10-14" version="2">MPTSNTIVLKSLSVLLGLFFIFVGTLKLTPAISKDLYKDLRTEYVKYAKVFPLTALFGVKIPSKWYRRTIGILEIVCGLAMALIPYHKVKNVANVTLLVLMLLGIYQHWMVSDPFERSGPALVFTFMLGGRLVVWYQTARLQDEMAAATQAPANGVKQD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-03-20" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M1D1</accession>
    <name>UFSP2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable Ufm1-specific protease 2</fullName>
            <shortName evidence="2">UfSP2</shortName>
            <ecNumber evidence="1 2">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA14252</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, a ubiquitin-like modifier protein bound to a number of target proteins. Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C78 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="1 2"/>
    <dbReference type="EMBL" id="CH379069">
        <property type="protein sequence ID" value="EAL30643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M1D1"/>
    <dbReference type="SMR" id="Q2M1D1"/>
    <dbReference type="FunCoup" id="Q2M1D1">
        <property type="interactions" value="541"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M1D1"/>
    <dbReference type="MEROPS" id="C78.A01"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0276352">
        <property type="protein sequence ID" value="FBpp0274790"/>
        <property type="gene ID" value="FBgn0074281"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812951"/>
    <dbReference type="eggNOG" id="KOG2433">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021066_1_0_1"/>
    <dbReference type="InParanoid" id="Q2M1D1"/>
    <dbReference type="OMA" id="CGLVKFG"/>
    <dbReference type="PhylomeDB" id="Q2M1D1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074281">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071567">
        <property type="term" value="F:deUFMylase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.130:FF:000001">
        <property type="entry name" value="Probable Ufm1-specific protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049387">
        <property type="entry name" value="UFSP2-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058757">
        <property type="entry name" value="UFSP2_MPN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153">
        <property type="entry name" value="UFM1-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153:SF2">
        <property type="entry name" value="UFM1-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26560">
        <property type="entry name" value="UFSP2_MPN_insect"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20908">
        <property type="entry name" value="UfSP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0788">Thiol protease</keyword>
    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="chain" id="PRO_0000280373" description="Probable Ufm1-specific protease 2">
        <location>
            <begin position="1"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="565"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q99K23"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9NUQ7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="608" mass="68497" checksum="7F54B6889904CE7D" modified="2006-02-21" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NQC4</accession>
    <accession>A0A6I8V778</accession>
    <name>A0A0R3NQC4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Sfp26Ad</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Sfp26Ad</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035268.1">
        <property type="nucleotide sequence ID" value="XM_015179782.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NQC4"/>
    <dbReference type="FunCoup" id="A0A0R3NQC4">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="GeneID" id="26532196"/>
    <dbReference type="KEGG" id="dpo:26532196"/>
    <dbReference type="InParanoid" id="A0A0R3NQC4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271183">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NQC4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015035268.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="12817" checksum="917E047D7469B1D3" modified="2016-01-20" version="1">MEISTEKQSLIMSRTERIAYFQIVLICSTLLHINGASIIDSLKYRLFQALSKAGFAVKAASMTGENIGEAMKNLSNVPLGIGMMINALFASGATEEKPPEDDPDCEKGGLPCKVMSIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBG0</accession>
    <name>A0A6I8UBG0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Neurotactin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">Nrt</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352826.2">
        <property type="nucleotide sequence ID" value="XM_001352790.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240580.1">
        <property type="nucleotide sequence ID" value="XM_033384689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBG0"/>
    <dbReference type="KEGG" id="dpo:4812606"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBG0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000295">
        <property type="entry name" value="neurotactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="365"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352826.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033240580.1"/>
        </source>
    </evidence>
    <sequence length="845" mass="92597" checksum="B434A340355A6496" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBQ2</accession>
    <name>A0A6I8UBQ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4812408</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352943.4">
        <property type="nucleotide sequence ID" value="XM_001352907.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UBQ2">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812408"/>
    <dbReference type="InParanoid" id="A0A6I8UBQ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBQ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000066">
        <property type="entry name" value="AGAP006059-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000143">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000065">
        <property type="entry name" value="Uncharacterized protein, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056707">
        <property type="entry name" value="DUF7805"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053207">
        <property type="entry name" value="Non-NMDA_GluR_Accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537:SF8">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47537">
        <property type="entry name" value="CUBILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25090">
        <property type="entry name" value="DUF7805"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1215"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="284"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="11"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="541"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1101"/>
            <end position="1122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001352943.4"/>
        </source>
    </evidence>
    <sequence length="1296" mass="140841" checksum="9BEB2DFBA2EA45DD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFW8</accession>
    <name>A0A6I8UFW8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">Agpat1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10">MV-25-SWS-2005</strain>
            <tissue evidence="9 10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001354866.3">
        <property type="nucleotide sequence ID" value="XM_001354830.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041747.2">
        <property type="nucleotide sequence ID" value="XM_015186261.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFW8"/>
    <dbReference type="KEGG" id="dpo:4815155"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFW8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004552">
        <property type="entry name" value="AGP_acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00530">
        <property type="entry name" value="AGP_acyltrn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434:SF11">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="7">
        <location>
            <begin position="92"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354866.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015041747.2"/>
        </source>
    </evidence>
    <sequence length="350" mass="38932" checksum="5A428E0FE68CFFF8" modified="2020-08-12" version="1">MSSFIELLGLFMLLMLPFLYETNHIFRYYFKFLIYYGIVSFNAIVLIPAFLTRPCDVRNLLWASTWCHRVTTLIGLRWELRGKEHLAKDQACIIVANHQSSLDVLGMFNIWHVMNKCTVVAKRELFYVWPFGLAAWLAGLIFIDRVRGEKAREILNDVNRRIKKQRIKLWVFPEGTRRNTGELHPFKKGAFHMAIDQQIPILPVVFSSYCTFLNDKKKILNAGRIVITTLPPVSTEGLTKDDIDPLMERVRAQMIETFKLTSAEALELRYNPLKSSPLAPSSSASSASEGVLPPGAEVEAAAVAAAASVAVASSGSSYAPLHVGAAAASATYETYKSGATAAKATMLKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFX3</accession>
    <name>A0A6I8UFX3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein penguin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">peng</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354871.3">
        <property type="nucleotide sequence ID" value="XM_001354835.4"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001356382.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033236910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033236911.1"/>
        </source>
    </evidence>
    <sequence length="785" mass="89080" checksum="A6117C8F577F19CC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJN4</accession>
    <name>A0A6I8UJN4_DROPS</name>
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        <submittedName>
            <fullName evidence="11">Basic helix-loop-helix transcription factor amos</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">amos</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001356551.4"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016360">
        <property type="term" value="P:sensory organ precursor cell fate determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="bHLH_TS_amos_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein atonal homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bHLH_dom"/>
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        <property type="entry name" value="bHLH_transcription_factors"/>
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    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF162">
        <property type="entry name" value="TRANSCRIPTION FACTOR ATOH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="146"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="83"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001356587.3"/>
        </source>
    </evidence>
    <sequence length="206" mass="23291" checksum="6FE7D1FF807B276B" modified="2020-08-12" version="1">MLTNNDLIEQFYYPSESHTDLDSHVQVQGMDAFQQLEQFMYQQELCGSGDSLSDETNSCSMEQLYYETPAVLGLEHMLSVQAEEHQHQQNQANSLGKCQQRSSNYWTKHQHQQHGSKLSTSISSSLSSSSSSSSSSPVCSGAVLKKRRLAANARERRRMNSLNDAFDKLRDVVPSLGHDRRLSKYETLQMAQAYIGDLVTLLSRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJY0</accession>
    <name>A0A6I8UJY0_DROPS</name>
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        <submittedName>
            <fullName evidence="5">BTB/POZ domain-containing protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4817451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356713.2">
        <property type="nucleotide sequence ID" value="XM_001356677.4"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
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        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410">
        <property type="entry name" value="HL07962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LD40565P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
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        <location>
            <begin position="304"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356713.2"/>
        </source>
    </evidence>
    <sequence length="515" mass="57015" checksum="37E20A015CF3EFEA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULP0</accession>
    <name>A0A6I8ULP0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4818178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357492.3">
        <property type="nucleotide sequence ID" value="XM_001357456.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULP0"/>
    <dbReference type="FunCoup" id="A0A6I8ULP0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4818178"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505:SF8">
        <property type="entry name" value="DPT-YFP REPRESSOR BY OVEREXPRESSION, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505">
        <property type="entry name" value="MADF DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="1">
        <location>
            <begin position="41"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357492.3"/>
        </source>
    </evidence>
    <sequence length="206" mass="24188" checksum="E404313AFF1A3961" modified="2020-08-12" version="1">MSCHRHVGAPKRITSAPIEPTASPITVKSPELTRRMMKYLKLIALYKDHECLWHQCHTDFFNLPLREKVWQEIAGKMKKGSDAEQWKNLMYKLRYSVELERIQQQAAKFQPDIDLAPKLQYGDKMQFLNHMFDRKWCKSPFCTKTSTPRRSECFMLLSDSTGLTSYARKLKALENLRNKCSPYVLSPEAFRKVQTLLEERELPAYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULQ7</accession>
    <name>A0A6I8ULQ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein sage</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sage</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357560.1">
        <property type="nucleotide sequence ID" value="XM_001357523.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULQ7"/>
    <dbReference type="SMR" id="A0A6I8ULQ7"/>
    <dbReference type="GeneID" id="4800354"/>
    <dbReference type="KEGG" id="dpo:4800354"/>
    <dbReference type="InParanoid" id="A0A6I8ULQ7"/>
    <dbReference type="OMA" id="QNPAWRE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071984">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11390">
        <property type="entry name" value="bHLH_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000090">
        <property type="entry name" value="Salivary gland-expressed bHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040259">
        <property type="entry name" value="Mesogenin/MesP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937">
        <property type="entry name" value="IP14615P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937:SF3">
        <property type="entry name" value="IP14615P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="186"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001357560.1"/>
        </source>
    </evidence>
    <sequence length="274" mass="29981" checksum="956CBC416ADD2A91" modified="2020-08-12" version="1">MTDQMLSAASGQPPPPALVALPVSTPGSYLNGAPEGDVEPVEQPVLLELGAPQSSHIPISLNYTLSPDGGGMLAYATHQQPPHGCLPYSPTLMSSYEKESSGMGMMQSTMAHPPYSIIPQPVSMWHAGQVPAPPLSSHMETKPNELVPPPMYMTYGGGSIASSTEVEISKEHNPAWREKALQMEKDYRRTACDRERTRMRDMNRAFDLLRSKLPISKPNGKKYSKIESLRIAINYINHLQAMLRDNSTQNESVCCTWSGGSSSPYDQGHDQWGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMP3</accession>
    <name>A0A6I8UMP3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4800724</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357944.2">
        <property type="nucleotide sequence ID" value="XM_001357907.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMP3"/>
    <dbReference type="FunCoup" id="A0A6I8UMP3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800724"/>
    <dbReference type="InParanoid" id="A0A6I8UMP3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001357944.2"/>
        </source>
    </evidence>
    <sequence length="495" mass="56911" checksum="65A045697A8B00AA" modified="2020-08-12" version="1">MNSPISKRCHHVMVALTDTNRRYHPELLEGTSLDDEFKILDRKSVLLRMPMASKSKQEEVPRYYRHLEFDAIGYECEPHVREQLSDLVGDMFRQRRHGCLLRLTPHRTNNMNLLTRLLVTEVDSIMLLYRYRLKCVNVEYFDLRKFDMANMLLQPGSAIRPSSRRQVQSGRTVQSTRELLQWLLNDFPAIRSRSVGDDYIDIQFVFRDGLHAVRQLHFTLFQIFGCEDRQDMADFFRDLPLGKQSNSTLLTDCIKESFDFQRPIGTLVMIELPTGRESQTAMTRKLLKLADVAYVSIVKAHQAATNPKARAKAVPASQSSTNLQKITPSCSFKNSFPSVSNISNSLVRRGGKLSSEDYNSLAYWYNRIDSKLLELQLSMEEHYMALYRQKGLTICSELRSMETDESAGDGQPPPMASSREQETACAYTELMKQFRKLEIDVKKTSFASTLNVYIGTKNHELAEMEKACKLKAIANMRLTLIEFMKPESEAEPHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMS1</accession>
    <name>A0A6I8UMS1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calmodulin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4800819</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358025.3">
        <property type="nucleotide sequence ID" value="XM_001357988.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMS1"/>
    <dbReference type="KEGG" id="dpo:4800819"/>
    <dbReference type="InParanoid" id="A0A6I8UMS1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358025.3"/>
        </source>
    </evidence>
    <sequence length="230" mass="26159" checksum="46D56D064184DD45" modified="2020-08-12" version="1">MSMDPTVSLEDLDRRRRRASSARKASQTPQPPPHLADSEAMAVINEIFNPSLKLPESSGHYTLPEEMSADDDVAPHELDISKLAELKEVFALFDTDCDGLISRDDLRFTYTALGNDPNEQVLEQMMLEAKEPLDYEAFVRLMSRRTQELDPEEVLLEAWSKWDDHGTGLIEERKIYEELTNYGDKMSLNEAKEALSHAPMAKPKSLEEPPMIDYPAFCRMLGGMRKRKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMY9</accession>
    <name>A0A6I8UMY9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Chymotrypsin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4800765</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357980.2">
        <property type="nucleotide sequence ID" value="XM_001357943.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMY9"/>
    <dbReference type="FunCoup" id="A0A6I8UMY9">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800765"/>
    <dbReference type="InParanoid" id="A0A6I8UMY9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000047">
        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1A"/>
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        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
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        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026057270" evidence="11">
        <location>
            <begin position="20"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="43"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001357980.2"/>
        </source>
    </evidence>
    <sequence length="273" mass="29662" checksum="D6EE32E92142ED35" modified="2020-08-12" version="1">MKFLLVTSVLLIIVSQCVAREIGVVRRHQRPHHLGTVKPETRVVGGTDAPAGFAPYQVSIMSTFGEHVCGGSIIGDRWILTAAHCLEWPTQYLKIVTGSNDYTKPGAEYLVDGAKVHCGHDKPAYHNDIALIHTAKPIVYNALTQPIQLASKGSLPKAGDTLTLTGWGSTRTWGRYVTDLQKIDLKYIAHSSCESKVRNINWLSEGHLCTFTQDGEGSCHGDSGGPLVDENGTLVGVVNWGEACAIGYPDVYGSVAYYRDWINEMMTEAGTAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN90</accession>
    <name>A0A6I8UN90_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4800913</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358102.3">
        <property type="nucleotide sequence ID" value="XM_001358065.4"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDZ"/>
    </dbReference>
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        <property type="entry name" value="PDZ_6"/>
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        <property type="entry name" value="SINA-like_animal"/>
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        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING"/>
    </dbReference>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SIAH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45877:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SINA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
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        <property type="entry name" value="Sina_RING"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="26"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
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        <location>
            <begin position="334"/>
            <end position="370"/>
        </location>
    </feature>
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        <location>
            <begin position="404"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001358102.3"/>
        </source>
    </evidence>
    <sequence length="497" mass="54579" checksum="613B9FA0A4726BB4" modified="2020-08-12" version="1">MKHTDRGTQPNAASTEGKATDNEAAAVRLLRIPRAAPSIENYGFHLTRSKWDPYPWVCEVATGTPAALCGLKTGDCVLEVNGVDVLGLRVADVAKIVKSQKDCVTILCWNSGCEKDCDKNSICCAPMPTSLRRLSVALESILRLIECPICNLTITPPAMQCQNGHVLCVDCRIRAERCPVCRDFYTPRRALLAEQIYFTIANAFEMCRSEDKLREKLFAGITKVPVAAVPQPAKTDADQSIWRKRRPLLPTNKFLTKLLAGRSDPLENLFESNAATLLRTNSTDNFNASNGTIQSAEPAEPTGTPRTTTMHPSHDEILGHLSLSTNDLRHATVKLKGQPNGESNVQVEGEGEEEEESGSSHSSSSVSFCYLPTRPQPSDSLLFIGKSRPIAPAQQPLHECMPPNVNESSSTLQLPAAETPPPSVTLSKCGTENSPEEVVQTKPTSASLLYCCPCECTLRFDDELTLNQDKDQQKQQQQKHQHQRGNCCCYKSAFRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNF6</accession>
    <name>A0A6I8UNF6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Beclin 1-associated autophagy-related key regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Atg14</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358292.2">
        <property type="nucleotide sequence ID" value="XM_001358255.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNF6"/>
    <dbReference type="FunCoup" id="A0A6I8UNF6">
        <property type="interactions" value="1516"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801157"/>
    <dbReference type="InParanoid" id="A0A6I8UNF6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097629">
        <property type="term" value="C:extrinsic component of omegasome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097632">
        <property type="term" value="C:extrinsic component of phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035032">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035014">
        <property type="term" value="F:phosphatidylinositol 3-kinase regulator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016240">
        <property type="term" value="P:autophagosome membrane docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018791">
        <property type="entry name" value="UV_resistance/autophagy_Atg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13664">
        <property type="entry name" value="BECLIN 1-ASSOCIATED AUTOPHAGY-RELATED KEY REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13664:SF0">
        <property type="entry name" value="BECLIN 1-ASSOCIATED AUTOPHAGY-RELATED KEY REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10186">
        <property type="entry name" value="ATG14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358292.2"/>
        </source>
    </evidence>
    <sequence length="496" mass="55827" checksum="83EB34E0957014FC" modified="2020-08-12" version="1">MASSSSSDESSKAPENFQISSASTNDDDDICDGTGYTKKKPNGGSSVKETSAAAASAAVGLRCPLCLGCSATRFHCRNCVRNGNITHSATERAESLSEKQQRYINLQAGLKTFSNRYERLIQQHKSNENRVMAIKAKRKQLDLLQQLIGSTTQRLLVLGERRDELRLANAEKRKNLPKYPIKVKMLEDYVLDRIEKIEKLRDTHLGLMDNIKQTARRGIQQLVTYIFPITEVVLKDEQQRKMSNERSEEAETIAALADAKNTSYIRGKWVFHGSGISEVQYRIVGPSLPANGDYTAYLDWLSDNKDDVPKATANEITPSRYEAYRIVGALTYTAQLTQLLSFYLNVRLPHKIAYGDFCRKLLNEEQFLRKVSRVNSNIMYLAYTQQVKLRALNEQHTLENILAILDLERSDLGRFGHLDVTNAPLMKSVDSLLIGIETATESESEDENSLRLDWESVPSLTPQPELADPNLMPAVAQQSTIAMVRMAATSMLRWIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQH5</accession>
    <name>A0A6I8UQH5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Nuclear envelope pore membrane protein POM 121</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4802373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359302.3">
        <property type="nucleotide sequence ID" value="XM_001359265.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239231.1">
        <property type="nucleotide sequence ID" value="XM_033383340.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802373"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="740"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="882"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1075"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1327"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="180"/>
            <end position="191"/>
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    </feature>
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            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
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            <begin position="531"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="751"/>
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        </location>
    </feature>
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        <location>
            <begin position="781"/>
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        </location>
    </feature>
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            <begin position="884"/>
            <end position="898"/>
        </location>
    </feature>
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    </feature>
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            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1337"/>
            <end position="1346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359302.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239231.1"/>
        </source>
    </evidence>
    <sequence length="1346" mass="137056" checksum="802B25330178FAC7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQI9</accession>
    <name>A0A6I8UQI9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dihydroxyacetone phosphate acyltransferase</fullName>
            <ecNumber evidence="14">2.3.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Glycerone-phosphate O-acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">Gnpat</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Dihydroxyacetonephosphate acyltransferase catalyzing the first step in the biosynthesis of plasmalogens, a subset of phospholipids that differ from other glycerolipids by having an alkyl chain attached through a vinyl ether linkage at the sn-1 position of the glycerol backbone, and which unique physical properties have an impact on various aspects of cell signaling and membrane biology.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>dihydroxyacetone phosphate + an acyl-CoA = a 1-acylglycerone 3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:17657"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57534"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="2.3.1.42"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:17658"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dihydroxyacetone phosphate + hexadecanoyl-CoA = 1-hexadecanoylglycerone 3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:40715"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:58303"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:40716"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of a heterotrimeric complex composed of GNPAT, AGPS and a modified form of GNPAT.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Peroxisome membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.42" evidence="14"/>
    <dbReference type="RefSeq" id="XP_001359337.4">
        <property type="nucleotide sequence ID" value="XM_001359300.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQI9"/>
    <dbReference type="FunCoup" id="A0A6I8UQI9">
        <property type="interactions" value="1550"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802414"/>
    <dbReference type="InParanoid" id="A0A6I8UQI9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078317">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008611">
        <property type="term" value="P:ether lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028353">
        <property type="entry name" value="DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF17">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500063">
        <property type="entry name" value="DHAPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000437">
        <property type="entry name" value="GPAT_DHAPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 18 22" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="20">
        <location>
            <begin position="181"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="18"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001359337.4"/>
        </source>
    </evidence>
    <sequence length="753" mass="85559" checksum="71A5523C95176F8D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQP2</accession>
    <name>A0A6I8UQP2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein Jhbp1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Jhbp1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359414.1">
        <property type="nucleotide sequence ID" value="XM_001359377.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQP2"/>
    <dbReference type="SMR" id="A0A6I8UQP2"/>
    <dbReference type="FunCoup" id="A0A6I8UQP2">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="GeneID" id="4802505"/>
    <dbReference type="KEGG" id="dpo:4802505"/>
    <dbReference type="InParanoid" id="A0A6I8UQP2"/>
    <dbReference type="OMA" id="PEYVKQC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070914">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000001">
        <property type="entry name" value="Takeout-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF41">
        <property type="entry name" value="RE70318P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026181943" evidence="4">
        <location>
            <begin position="20"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359414.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="27193" checksum="A5222203AF36FB09" modified="2020-08-12" version="1">MLKYVCLVIVLQTLWMVQAEAPAYIKQCHRDDPKLVDCFMGAIEHLKPYLASGIPEIQLPSVEPFKMDSLALQLTEGPQGYKITLKNMEAFGASNFQVKSLKLTENNEPFKAKIVMPKLKIEAKYTSSGVLLILPASGGGDFHAVFEGVSADLTGKTSVRASKGANYLHIDSLSLVLDVKDVKMSISGAFNNNRILLEATNLFLRDNSQIVLEAMQSQLQKKLASEFGKLANQLLKNVPIEQFYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URK5</accession>
    <name>A0A6I8URK5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL19m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L19, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mRpL19</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359806.2">
        <property type="nucleotide sequence ID" value="XM_001359769.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URK5"/>
    <dbReference type="FunCoup" id="A0A6I8URK5">
        <property type="interactions" value="903"/>
    </dbReference>
    <dbReference type="GeneID" id="4803001"/>
    <dbReference type="KEGG" id="dpo:4803001"/>
    <dbReference type="InParanoid" id="A0A6I8URK5"/>
    <dbReference type="OMA" id="IHEIQVV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080773">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.790:FF:000002">
        <property type="entry name" value="39S ribosomal protein L19, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="34"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359806.2"/>
        </source>
    </evidence>
    <sequence length="319" mass="37001" checksum="691A64FEBBA4A7FD" modified="2020-08-12" version="1">MNSARAMSKVWAQQSAFNRIVTFSTKATQHAAAAVSSAGAEPPGEEKTSATAPAAAQPPTPTPTPVRKPIVPSNYRFIYPEFLPDPKVEWRNPIREKLERLDMLDRRRQIDLPEFYVGSIMAVTSSDPHAAGKTSRFVGICINRDRCGLRARFVLRNVIDHQGMEVVYELYDPTIQKVEVLRLEKRLDDNLFYLRDALPEFSTFDENMEAEHLEEGAPVPVNDIKVILRPRPWLERWERQNLRGVANIDQYIKDKHRKSAEKVQKPWEKYDLMKQYRSTIPEEEQKEVFAEVHTELHSLELQRKRNKRKRTFVKPKQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URZ2</accession>
    <name>A0A6I8URZ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger protein BRUTUS-like At1g18910</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4803154</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359944.5">
        <property type="nucleotide sequence ID" value="XM_001359907.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URZ2"/>
    <dbReference type="FunCoup" id="A0A6I8URZ2">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803154"/>
    <dbReference type="InParanoid" id="A0A6I8URZ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="68"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="120"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359944.5"/>
        </source>
    </evidence>
    <sequence length="164" mass="18794" checksum="D4095519F160DDBB" modified="2020-08-12" version="1">MVYLNVIIGIVTVLVTAGFAVYMSQPTYQTEPPHQRRRNEEKGAKNGSRPKNNFENQRVRESQPSDLCPLCQIEMSSSNMHRMQCGHALHNDCFQEFRYLRRSCLLCNHIVNPSLPGDKCTICLDPLTKKNMKHFPCQHAMHNDCFSQYLASGATNCPLCRSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USH1</accession>
    <name>A0A6I8USH1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">23 kDa integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TM4SF</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360223.4">
        <property type="nucleotide sequence ID" value="XM_001360186.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USH1"/>
    <dbReference type="FunCoup" id="A0A6I8USH1">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803510"/>
    <dbReference type="InParanoid" id="A0A6I8USH1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070958">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03156">
        <property type="entry name" value="uroplakin_I_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000032">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF505">
        <property type="entry name" value="TRANSMEMBRANE 4 SUPERFAMILY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001360223.4"/>
        </source>
    </evidence>
    <sequence length="309" mass="35026" checksum="ED4AA215F815005E" modified="2020-08-12" version="1">MQTARYSTESTRKFEDSRRTTTMALPKKIKCFKYLVYSYVFLLALTGAAQIFLGTSLLWGHSVYYGIVQNKLWAPAAILLCLGPVTFILCWMGCQATNQRKRCLLGMFSALLVACICVQFIICGWSLAMRENLPTSVEIFIDDSFVEFLDKFSRTKVDNLHLWNRMQSQLQCCGVDGPLDYRRLSLPWSCCSRPEHAYESACDTHYKRGCLAVVSEQIKNRLLITAFGAAIIAIFQSLGIFCAVHLTILFGKNDNTHPINMNRKKKQQQFLPLTIQDKRHDLPSPINLSPSAPGQRVLKTNLPSAMQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USY0</accession>
    <name>A0A6I8USY0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dentin sialophosphoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4803561</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPOT14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360268.1">
        <property type="nucleotide sequence ID" value="XM_001360231.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USY0"/>
    <dbReference type="FunCoup" id="A0A6I8USY0">
        <property type="interactions" value="331"/>
    </dbReference>
    <dbReference type="GeneID" id="4803561"/>
    <dbReference type="KEGG" id="dpo:4803561"/>
    <dbReference type="InParanoid" id="A0A6I8USY0"/>
    <dbReference type="OMA" id="QFSKDCI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075473">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046890">
        <property type="term" value="P:regulation of lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053719">
        <property type="entry name" value="Lipogen_MT_Stabilize_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009786">
        <property type="entry name" value="Spot_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14315:SF17">
        <property type="entry name" value="MIP21584P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14315">
        <property type="entry name" value="SPOT14 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07084">
        <property type="entry name" value="Spot_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001360268.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="30675" checksum="6AD1C55CEC2EF014" modified="2020-08-12" version="1">MSGYTDLTKLETSRNCLRRIARHDEQQFSKDSIVNVMEKFVKTVNIMDDTILVPCRLMDRQIGDSTDIIPASGKDTTANQLTQSSSAQGKRGAAHSKNVHEFLSASELFNLYSMLNALKKDLLWTANQEDDEAVEMESHQQQQRTASPVANSSESKTESNPSSTTSASSSIKGHVRRTSTASMMSTTSVSNMSDSDSDISQENDSGLESDGNKSDHAQSSDGSDIVDDACHKSKQASGDKATELAKRCRRHLNGLYQCLEQMTEAANYLTARYQSDIGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUD6</accession>
    <name>A0A6I8UUD6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Or59a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361038.2">
        <property type="nucleotide sequence ID" value="XM_001361001.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUD6"/>
    <dbReference type="FunCoup" id="A0A6I8UUD6">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804482"/>
    <dbReference type="InParanoid" id="A0A6I8UUD6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361038.2"/>
        </source>
    </evidence>
    <sequence length="379" mass="44162" checksum="7E382BDB921AB4A5" modified="2020-08-12" version="1">MPEEAIDSLSFIRSHWTAWWILGVAHKRVQSWRSLYVCLSVLANVMVTVGYPLHLALSLFRNRTLTEDILNLTTFVTCLACSAKCLIYAYNIDKVARMEQLLRLLDERVSGKEQRAIYGNVKLQLRIVLYVFIGIYMPCGLFAELSFLFKEQRGLMYPAYFPFDWLTSTRNYYMANVYQIVGISFQLLQNYVSDCFPAVVLCLISSHVKMLYSRFEEIGRDPSINAEKQLEDCITDHKRLLELFRCIERLMSVPMFIQFTVTALNVCISIAALVFYVSEPMARTYLIFYALAMPLQIFPSCYFGTDNEFWFGRLHYAAFSCDWPKQGRSFKRKMMFFVERSLKQSTAIAGGMLRIHLDTFFSTLKMAYSLFTIIIRMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUX2</accession>
    <name>A0A6I8UUX2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Otefin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Ote</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361297.2">
        <property type="nucleotide sequence ID" value="XM_001361260.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUX2"/>
    <dbReference type="FunCoup" id="A0A6I8UUX2">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804789"/>
    <dbReference type="InParanoid" id="A0A6I8UUX2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd12934">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011015">
        <property type="entry name" value="LEM/LEM-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003887">
        <property type="entry name" value="LEM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03020">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00540">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63451">
        <property type="entry name" value="LEM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50954">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="LEM" evidence="3">
        <location>
            <begin position="4"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361297.2"/>
        </source>
    </evidence>
    <sequence length="415" mass="45693" checksum="94A62AEC98C640F9" modified="2020-08-12" version="1">MADVDDFDTISDAELRAKMIAQGLPNIPVTSSSRKVLVKRLRASIGGQASPAVTASPKKTNKRETLAPVAAPASASTPVDKPDGTKPGPGSTKSRRTIASTANDAKAADRRRPDEQVVKKPVVAAPTQTRRISTSSEKREVVSERPVKKPESIIEEAAVPKRTDYHENTLEVNSTIVLESDEEEDEQLARAADLVELQYKAKEKPKPRSKLTTTTTNTFEYSGKTAFSMAPLVSTSKAADPPRRQAYESGAPTSTAFRTQTSSSSYDYLNNPTGRYSSYVRSPAQSYVTAEAPAPSLAYTSSYRRSPPRQPFANELSDENDAEDSQYESTFARNLARLRSEKIGDRNSQYRRTIASSNSMGYEPQARRSLRPDETSVSVAFRGWLNSLNEAYNLKSNMFILFVVILLIGVYFLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV00</accession>
    <name>A0A6I8UV00_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC5495503</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361310.3">
        <property type="nucleotide sequence ID" value="XM_001361273.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV00"/>
    <dbReference type="FunCoup" id="A0A6I8UV00">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:5495503"/>
    <dbReference type="InParanoid" id="A0A6I8UV00"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF4">
        <property type="entry name" value="FI01423P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="89"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361310.3"/>
        </source>
    </evidence>
    <sequence length="221" mass="25159" checksum="FC88BDF8B9864599" modified="2020-08-12" version="1">MGKISLYGLDASPPTRACLLTLKALELPFEYVFVNLLTKENFAEDYMKKNPQHTVPMLQDDDDYIWDSHAIMAYLVGKYAETDELYPKDLLQRAVVDQRMHFESGVIFEGALRRLTRPVLFLGGTTLPQANVDQVNEVYDFLETFLDNDFVAGDHLTIADFSIVSSISSLGVYLEHDPVKYPKTTAWLERLKELPYYEEANGSGATQFMQLIKSKNFTIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVY5</accession>
    <name>A0A6I8UVY5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Carbonic anhydrase 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CAH13</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361744.3">
        <property type="nucleotide sequence ID" value="XM_001361707.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVY5"/>
    <dbReference type="FunCoup" id="A0A6I8UVY5">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805326"/>
    <dbReference type="InParanoid" id="A0A6I8UVY5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03122">
        <property type="entry name" value="alpha_CARP_receptor_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041887">
        <property type="entry name" value="Alpha_CARP_receptor-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF227">
        <property type="entry name" value="CARBONIC ANHYDRASE 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="8">
        <location>
            <begin position="65"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361744.3"/>
        </source>
    </evidence>
    <sequence length="474" mass="52837" checksum="78386DCD12FF8A4E" modified="2020-08-12" version="1">MFDTTLGYLIGLVMQLFWLLVSQGFCQMVQHPLLASVLCLVLGLAQQTGQKILRERFRWVLQYPKIWITHSDLATDDCNSWYDMADQREGGLEGLDGAGFQSPINIDDRQLSPMAIRELLNWNHYDELPASIQVENTGHTLILRAQFHGNTPTISGADLLASYTFVEVRFHWGWCNSEGSEHTFNHRKFPMEMQVMHRASSGMSRSCTSSYDLLTIAYMFELSAHNPFLDPLVQNLRQVQQPGKAVNISPFPLCYLMPAFRAGFYSYGGSLTRPPFYEGTEWFIFPETLAISDFQLRHFRQLLSHDGVTPITRNARPVQALGNRSINLNCFCPMNTNKLASVPVVGLCQQQQQAADVQKEEPVEKVQEQQRGPAPAGSEEEGELIESMDTTTVITSDSTNMSLTTFLRKEQLTPTQQDYSSTAIVIHSDSTSSDMCAPHPVGGPGSAQQASCNADAICGVGDGINIPVYHGQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVY8</accession>
    <name>A0A6I8UVY8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Periodic tryptophan protein 2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4805310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat PWP2 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001361693.3"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8UVY8">
        <property type="interactions" value="1046"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805310"/>
    <dbReference type="InParanoid" id="A0A6I8UVY8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001412">
        <property type="entry name" value="AGAP010693-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000499">
        <property type="entry name" value="PWP2, small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000541">
        <property type="entry name" value="PWP2, small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027145">
        <property type="entry name" value="PWP2"/>
    </dbReference>
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        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="389"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="431"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="517"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="6">
        <location>
            <begin position="793"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="902"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="902"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="935"/>
            <end position="948"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361730.2"/>
        </source>
    </evidence>
    <sequence length="948" mass="105728" checksum="0ADA2F7554068D8D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW18</accession>
    <name>A0A6I8UW18_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor SOX-15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Sox15</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361762.2">
        <property type="nucleotide sequence ID" value="XM_001361725.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW18"/>
    <dbReference type="FunCoup" id="A0A6I8UW18">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805348"/>
    <dbReference type="InParanoid" id="A0A6I8UW18"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22032">
        <property type="entry name" value="HMG-box_SoxF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000008">
        <property type="entry name" value="transcription factor SOX-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF317">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="216"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="5">
        <location>
            <begin position="216"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="694"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="429"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="696"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="716"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361762.2"/>
        </source>
    </evidence>
    <sequence length="782" mass="84264" checksum="BAF431E367499F74" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX50</accession>
    <name>A0A6I8UX50_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132419.2">
        <property type="nucleotide sequence ID" value="XM_002132383.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX50"/>
    <dbReference type="KEGG" id="dpo:6903544"/>
    <dbReference type="InParanoid" id="A0A6I8UX50"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20987">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20987:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026329227" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132419.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="10001" checksum="ACED76DE664620F1" modified="2020-08-12" version="1">MKAALVFLFLALFVAAVHGASDCDPDGNGKPTCQSGNIGERFRNNWDPTRYWLCAEAGEPQVVLCDQTGYDPVSKECVSWSEWSWYPPCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXR1</accession>
    <name>A0A6I8UXR1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6903075</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_002132793.2">
        <property type="nucleotide sequence ID" value="XM_002132757.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXR1"/>
    <dbReference type="KEGG" id="dpo:6903075"/>
    <dbReference type="InParanoid" id="A0A6I8UXR1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026022257" description="aspartate transaminase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="65"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002132793.2"/>
        </source>
    </evidence>
    <sequence length="462" mass="51752" checksum="9122AC970476E50A" modified="2020-08-12" version="1">MSAILSVWHSLLLQTVVKSSSHRSAATGRRRNDGERLSPFACLKRATPTEMENLNQDYQLDSSPRKMNLAHEVYRNEVGKAQTFQAVKKAEISIACNDFNSSEPLPPLGNPDFLHAATEMVLGKSSPAQAVGRVVGIQTKNSIDALALAAQFLRHKTTRDVCLLARPCNPIYWQIFKEAGFNCQRYQYYSDARGELNIYGLVADLMTSPDGAVVVLDACSQHPTGQDPSVDEWKLLFHIVKCKKMLPLIHLDSPGLASGDPNEDAWPVRFFSDCGIDVLCAQSFVKNFGLYNETVGHLMVVLSNVAQVHAFRGQFESMLLDKNAECSSVHSSRIVAKILTDEKLRSEWCQTLKDMHQRVSRMRGEIRKKLAELKTPGNWQNLVKQNGYHLYWNPSRSQIRQLKDRHIYVPSSGRMNLTSVSPTNLDSLAAALNDVMQATKGDVSSDNYFVADKFNTQYFHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V198</accession>
    <name>A0A6I8V198_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135475.2">
        <property type="nucleotide sequence ID" value="XM_002135439.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V198"/>
    <dbReference type="KEGG" id="dpo:6900124"/>
    <dbReference type="InParanoid" id="A0A6I8V198"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V198">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002135475.2"/>
        </source>
    </evidence>
    <sequence length="248" mass="28806" checksum="679B8BC8178C23B9" modified="2020-08-12" version="1">MNETVDEEVFIAQIATTTGSTPLQNIFPPLHEHLIKYAVRFGRLLYIHDVSSWNEWSTDLAEFKDLTPHEACSKFLKDVLPIVNTYKLSLTVKKRLRLLDHHPCFRRTGEGYQYAPKCEPSNRFLLQPSRDYCLASALPTSIDSRLASHNVIISNEAFERKASVGYNYKVSDEFASLFSAMVSTDFELSHGYRHDLDEIHYRLRRARQSFEDTARFVPFRRSLDQLVYNIEIFGKLKKKVMDSFNTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1C2</accession>
    <name>A0A6I8V1C2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extensin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6900045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135409.2">
        <property type="nucleotide sequence ID" value="XM_002135373.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1C2"/>
    <dbReference type="KEGG" id="dpo:6900045"/>
    <dbReference type="InParanoid" id="A0A6I8V1C2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026344798" evidence="2">
        <location>
            <begin position="19"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002135409.2"/>
        </source>
    </evidence>
    <sequence length="173" mass="18641" checksum="E24B79037EC82731" modified="2020-08-12" version="1">MKFFILATFGMLLSLAAGDVSHLPLELLEEHQQEHQGYGYDYPKPVVPFVVVTTTTEPPTPPPTYLPPKPVPTYLPPPPPTTTTTTTTTTTPAPTPAPTYLPPPPPTTTTTTPPPTPAPTYLPPPPPTTTTTTPAPTPAPIYLPPPQPEPQPEPEPEEEHGYHYEAPAKAFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1T8</accession>
    <name>A0A6I8V1T8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6896656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002136872.2">
        <property type="nucleotide sequence ID" value="XM_002136836.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1T8"/>
    <dbReference type="KEGG" id="dpo:6896656"/>
    <dbReference type="InParanoid" id="A0A6I8V1T8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026327783" evidence="1">
        <location>
            <begin position="25"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002136872.2"/>
        </source>
    </evidence>
    <sequence length="71" mass="7629" checksum="7FE7D8C0FAA2EB5B" modified="2020-08-12" version="1">MAHTKGFLLSLMAVLVLLLSTASGSPARGYFGSPQRGGRSYTDIARVVNPNPYAFVGSRNYPGQPFWPVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2I1</accession>
    <name>A0A6I8V2I1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Uridine diphosphate glucose pyrophosphatase NUDT14</fullName>
            <ecNumber evidence="9">3.6.1.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nucleoside diphosphate-linked moiety X motif 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6897081</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and ADP-ribose to ribose 5-phosphate and AMP. The physiological substrate is probably UDP-glucose. Poor activity on other substrates such as ADP-glucose, CDP-glucose, GDP-glucose and GDP-mannose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.</text>
            <dbReference type="EC" id="3.6.1.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.1.45" evidence="9"/>
    <dbReference type="RefSeq" id="XP_002137265.3">
        <property type="nucleotide sequence ID" value="XM_002137229.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2I1"/>
    <dbReference type="FunCoup" id="A0A6I8V2I1">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897081"/>
    <dbReference type="InParanoid" id="A0A6I8V2I1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008768">
        <property type="term" value="F:UDP-sugar diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18887">
        <property type="entry name" value="NUDIX_UGPPase_Nudt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000035">
        <property type="entry name" value="Uridine diphosphate glucose pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004385">
        <property type="entry name" value="NDP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00052">
        <property type="entry name" value="nudix-type nucleoside diphosphatase, YffH/AdpP family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF15">
        <property type="entry name" value="URIDINE DIPHOSPHATE GLUCOSE PYROPHOSPHATASE NUDT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="12">
        <location>
            <begin position="63"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002137265.3"/>
        </source>
    </evidence>
    <sequence length="236" mass="26394" checksum="6D2975A60733E98E" modified="2020-08-12" version="1">MLKFFRGGLRRVCCFDAKKSAFGRLSNITRIWFSPLPAKSQWIKPCCVLYLENEMKKETHIVKVLDGVLVVLYNKSRDKLIFVRQFRAAVYQAQLSRKQPITKGDVDLQKFPPELGITLELCAGQVDKDQSLKNIAREEILEECGYDVPTDSIQPIFHYRSGVGISSGAQALFYCEVCDEQRVSAGGGVDKEVIQVVELSLDEARELVKTGMTTNGGPALLVGVMWFLLNKAPKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3G9</accession>
    <name>A0A6I8V3G9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Polycomb protein Scm</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Scm</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137925.2">
        <property type="nucleotide sequence ID" value="XM_002137889.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3G9"/>
    <dbReference type="FunCoup" id="A0A6I8V3G9">
        <property type="interactions" value="1106"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897820"/>
    <dbReference type="InParanoid" id="A0A6I8V3G9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20110">
        <property type="entry name" value="MBT_dScm_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09578">
        <property type="entry name" value="SAM_Scm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000100">
        <property type="entry name" value="Polycomb protein Scm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.190:FF:000004">
        <property type="entry name" value="Polycomb protein Scm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000016">
        <property type="entry name" value="polycomb protein SCMH1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.190">
        <property type="entry name" value="SLED domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004092">
        <property type="entry name" value="Mbt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047531">
        <property type="entry name" value="SAM_Scm-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021987">
        <property type="entry name" value="SLED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038348">
        <property type="entry name" value="SLED_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012313">
        <property type="entry name" value="Znf_FCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010507">
        <property type="entry name" value="Znf_MYM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF132">
        <property type="entry name" value="POLYCOMB PROTEIN SCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02820">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12140">
        <property type="entry name" value="SLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06467">
        <property type="entry name" value="zf-FCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00561">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51079">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51024">
        <property type="entry name" value="ZF_FCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FCS-type" evidence="16">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="13">
        <location>
            <begin position="162"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="13">
        <location>
            <begin position="268"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="15">
        <location>
            <begin position="786"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="524"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="633"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="581"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="634"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="657"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002137925.2"/>
        </source>
    </evidence>
    <sequence length="857" mass="91608" checksum="4F86E5392215863B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3P3</accession>
    <name>A0A6I8V3P3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein Ir41a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ir41a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138113.2">
        <property type="nucleotide sequence ID" value="XM_002138077.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3P3"/>
    <dbReference type="FunCoup" id="A0A6I8V3P3">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="GeneID" id="6898024"/>
    <dbReference type="KEGG" id="dpo:6898024"/>
    <dbReference type="InParanoid" id="A0A6I8V3P3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245983">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050907">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000312">
        <property type="entry name" value="Ionotropic receptor 41a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF40">
        <property type="entry name" value="IONOTROPIC RECEPTOR 41A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="610"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="10">
        <location>
            <begin position="345"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002138113.2"/>
        </source>
    </evidence>
    <sequence length="647" mass="73592" checksum="519FEE0E365581C1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4R1</accession>
    <name>A0A6I8V4R1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="11">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6898836</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable phosphatase which plays a redundant role with gsp-4 in spermatogenesis by regulating sister chromatid segregation during meiosis. In addition, involved in sperm motility by controlling the dynamic disassembly of major sperm proteins (MSP) in the spermatozoan pseudopodium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Pseudopodium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="11"/>
    <dbReference type="RefSeq" id="XP_002138809.1">
        <property type="nucleotide sequence ID" value="XM_002138773.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4R1"/>
    <dbReference type="FunCoup" id="A0A6I8V4R1">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="GeneID" id="6898836"/>
    <dbReference type="KEGG" id="dpo:6898836"/>
    <dbReference type="InParanoid" id="A0A6I8V4R1"/>
    <dbReference type="OMA" id="MGWSDND"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246386">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V4R1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031143">
        <property type="term" value="C:pseudopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097723">
        <property type="term" value="P:amoeboid sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018991">
        <property type="term" value="P:egg-laying behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007060">
        <property type="term" value="P:male meiosis chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031272">
        <property type="term" value="P:regulation of pseudopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="12">
        <location>
            <begin position="122"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002138809.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="35230" checksum="CA19CCD875956660" modified="2020-10-07" version="1">MTASVVATDAGNIDQLIHKIIDVRRAKQLSLSESEIRGICMRSREVFLSQPMLLELSAPVKICGDIHGQFTDLLRLFDYGGYPPSSNYLFLGDYVDRGKQSIETLCLLLAYKIKFPENFFLLRGNHESAGINRIYGFYDECKRRYTIKLWRTFVDCYNCMPVSAVVDEKIFCCHGGLSPDLLSMSQIGQLSRPCDVPDKGLLCDLLWSDPDPKIMGWSDNDRGVSVTFGADIVGKFVHRNKFDLICRAHQVVEDGYEFFAKRQLITIFSAPNYCGEFDNAGAMMSVDETLMCSFYVLKPSKKAGMRKVHSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4Y8</accession>
    <name>A0A6I8V4Y8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial import receptor subunit TOM40 homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tomboy40</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138976.2">
        <property type="nucleotide sequence ID" value="XM_002138940.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4Y8"/>
    <dbReference type="KEGG" id="dpo:6899051"/>
    <dbReference type="InParanoid" id="A0A6I8V4Y8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002138976.2"/>
        </source>
    </evidence>
    <sequence length="325" mass="35252" checksum="4F41420AAF2CB886" modified="2020-08-12" version="1">MVFASGGRQPPPPPSPPLSPPSSYQSWQKPSAPKPESLENPGTVEELFNKVRETQPNCFEGAKIMLNKALSNHFQMSHTIHINNTSPSYHFQSIYAGTQKHTATEAYPVLLADLDGQGNLNCNVIHQVSKRLRCKIGADFQPGAQNTPNQATADYRGDDCTFSLTMASPGLATRCGMIVASLLQSVAPRWAVGAELAYQCGYNVPGGQNAIVSMVGRYDNGQSMSCAKIGPTGIHLGHYRKCSDQLQIGLEVETCLRTMASVATIAYQSKIQKGDVQFRASLDTGWRVCGELEKRLQHSPFTFLLSGCMMHKTGQFKMGAGVIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5A1</accession>
    <name>A0A6I8V5A1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein prim</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">prim</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138320.1">
        <property type="nucleotide sequence ID" value="XM_002138284.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5A1"/>
    <dbReference type="FunCoup" id="A0A6I8V5A1">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="6898254"/>
    <dbReference type="KEGG" id="dpo:6898254"/>
    <dbReference type="InParanoid" id="A0A6I8V5A1"/>
    <dbReference type="OMA" id="TCMMVCG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245854">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138320.1"/>
        </source>
    </evidence>
    <sequence length="52" mass="5953" checksum="EF7340E883693720" modified="2020-08-12" version="1">MCVYLGATDDLMEITFFVLCRVLELSLFTCMMICNAIKSKWNNTKAIEMAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6E9</accession>
    <name>A0A6I8V6E9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_004444265.2">
        <property type="nucleotide sequence ID" value="XM_004444208.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6E9"/>
    <dbReference type="GeneID" id="6898575"/>
    <dbReference type="KEGG" id="dpo:6898575"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245703">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031931">
        <property type="entry name" value="DUF4768"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15989">
        <property type="entry name" value="DUF4768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026160834" evidence="1">
        <location>
            <begin position="26"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_004444265.2"/>
        </source>
    </evidence>
    <sequence length="185" mass="21239" checksum="A02433C4F78DB892" modified="2020-08-12" version="1">MQDTPALCLLSALLAVSLLSSRAYARRLSSPDALPFPGEQDMIIPQSAIAIPTPTAKATASTVGSVRVNSTSSREIQKDSTNLFARAATEGGSAVKHIRRAKREFFVDTPFDFHTMHLFCDIDMLGRHRLIWFFPNHCIWVWNNKYAHEGFYRLYKMYQLEAFFFGQWNVRLFQYELEKATFYAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V893</accession>
    <name>A0A6I8V893_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">Protein croquemort</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">crq</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11 12">MV-25-SWS-2005</strain>
            <tissue evidence="10 11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356433.2">
        <property type="nucleotide sequence ID" value="XM_001356397.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035817.2">
        <property type="nucleotide sequence ID" value="XM_015180331.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035818.2">
        <property type="nucleotide sequence ID" value="XM_015180332.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V893"/>
    <dbReference type="KEGG" id="dpo:4817152"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF114">
        <property type="entry name" value="FI02050P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356433.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015035817.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015035818.2"/>
        </source>
    </evidence>
    <sequence length="489" mass="55550" checksum="50A7DE1356FEA306" modified="2020-08-12" version="1">MCCKCCGETQRKAWVFGLGSLFMILGILIVVFWPGLADHLVEDGLKLKPGTDTYDSWLEAPIPIYLSFNMFNWTNPEEIRNPNVKPNFVEMGPYVFLEKHKKENFTFYENATVAYYERRTWFFDAEKSNGTLDDMVTAAHAITATVADEMRHQKKILKKIINFMLNHEGGELYTTKPVGEWIFKGYQDNLTDFLNLFNTSVIDIPYKRFGWLADRNESLTYDGLFTIHTGTDNIANVGKLTHWNGKAETGFYEMPCGVVNGTTGDLFAPKMNVKDEITIFATDACRFMNLRPEGTLENHGLTATRWVGTEETLDSGENYPNQACFCDPRLEECPKTGVVECKACRDKAPIYSSFPHFYLADKHYLDAITGMNPDKAKHEFVMAIEPTTGVPVQVHGRIQINMMIEPDDDYDIYRGVSKVLMPMFWFDQYAELSPELASKAKLAINLSSYGIIFGYSLVGFASLVIITGVALTLTKRWVRRDDDEAMLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8R2</accession>
    <name>A0A6I8V8R2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Facilitated trehalose transporter Tret1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC26533486</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015036171.2">
        <property type="nucleotide sequence ID" value="XM_015180685.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036172.2">
        <property type="nucleotide sequence ID" value="XM_015180686.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V8R2"/>
    <dbReference type="KEGG" id="dpo:26533486"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000218">
        <property type="entry name" value="facilitated trehalose transporter Tret1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF33">
        <property type="entry name" value="AT22075P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="32"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015036171.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015036172.2"/>
        </source>
    </evidence>
    <sequence length="469" mass="52370" checksum="5BC595DE09E081C2" modified="2020-08-12" version="1">MRHYQKTFRLNEMTRVFQNSLLQAKTRYQLLATVIVNIITFGHGVGVGWLSPTLTRIQAPDSPLDFAVNIDEISWLGSMIGLGNLAGNLAIAFLLERTGRKFCIYLLAGPYACLWILVYCASNVGYLYTARFLCGFTGGALYIVVPIFISEVADPSIRGALSSVMMMYFNFGVLAGYTMSTYLPYHVVPIVGIMLPIAYFFASLMLPETAPYLLKRSQLNAAEKSFRYYTNQQGGVTSKAQFEDLRSAVLLQQSQSTSHLSYKDLITKPALKAFGAAAVLCAGLELSNVYSFINYMSDIFTSSGSLLDVNTCTIIIGVVQIIGVYTSTIFVDIVGRRFLMLISTLGVGIGCIAFGCFTYFAEHYDLSQFNWLPLALMILIIYFANIGINSLFCLVMVELFPSKIRSLATSMSLIWMSLLIFCTLKLFPYLMHNWGISITMWFSATWSLLTFIYFLLFLPETKGKSMIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9M6</accession>
    <name>A0A6I8V9M6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Limbic system-associated membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DIP-eta</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015036696.2">
        <property type="nucleotide sequence ID" value="XM_015181210.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9M6"/>
    <dbReference type="FunCoup" id="A0A6I8V9M6">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902591"/>
    <dbReference type="InParanoid" id="A0A6I8V9M6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V9M6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000328">
        <property type="entry name" value="CLUMA_CG000981, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000376">
        <property type="entry name" value="CLUMA_CG000981, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000392">
        <property type="entry name" value="CLUMA_CG000981, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF253">
        <property type="entry name" value="DPR-INTERACTING PROTEIN ETA, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026169157" evidence="11">
        <location>
            <begin position="38"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="50"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="152"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="248"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="359"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="375"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015036696.2"/>
        </source>
    </evidence>
    <sequence length="460" mass="50758" checksum="644C51C90926DB31" modified="2020-08-12" version="1">MYTISTQCQSPANGFRLANAIKVVLLVVILMCQQCHLQRVEVPPEAIVDPKFSSPIMNMTAPVGRDAFLTCVVQDLGPYKVAWLRVDTQTILTIQNHVITKNQRIGIANSEHKTWTMRIRDIKESDKGWYMCQINTDPMKSQMGYLDVVVPPDILDYPTSTDMVVREGSNVTLKCAATGSPEPTITWRRESGVPIELSSGEEVPSVEGTDLIIPNVKRQHMGAYLCIASNGVPPSVSKRITLIVHFAPMITVQNQLIGAVEGKGVTLECESEAYPKSINYWTRERGEIVPPGGKYSANVTEIGGYRNSMKLHINPLSQAEFGAYRCVAKNSLGDTDGTIKLYRIPPNAVNYVENFEARHKGKKRTKSSEMYHPARAQEHSGEDMENPGKRKADLSLSAESIDSMYGASSSAVVQHQTLNRLLLILVLALTTTTTVATMATATGMMRKTQLPLLPQTQTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAV6</accession>
    <name>A0A6I8VAV6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heterogeneous nuclear ribonucleoprotein A3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036845.2">
        <property type="nucleotide sequence ID" value="XM_015181359.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAV6"/>
    <dbReference type="FunCoup" id="A0A6I8VAV6">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816615"/>
    <dbReference type="InParanoid" id="A0A6I8VAV6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VAV6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026343385" evidence="2">
        <location>
            <begin position="21"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036845.2"/>
        </source>
    </evidence>
    <sequence length="263" mass="27000" checksum="76DF037034C4FC97" modified="2020-08-12" version="1">MWKYLLICVVMSALNGESSAAGQQPRRRRGYAGGYAGGFASSGGGAGGYTGTYNSGGGGVGHSSYVGTGGGSGGYYDYDDYDGNSPNFGIIDPYLFHQQLTNHILAQNYANQQAITGLATAGNAFASADASLVDDDDTRIQQQIAAQQAYHDNLVRQNRYRGSGSGSGSSSGSYNSHRYAPSYAAASGSIGPNGHRQTAYISPANPASPNIVNRFGGGSSSGGGYKGVSVSSYSHSNGDGTSRRGAQTTVNDNGKVTSYSTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VB80</accession>
    <name>A0A6I8VB80_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Golgin-84</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">Golgin84</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359134.2">
        <property type="nucleotide sequence ID" value="XM_001359097.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037645.2">
        <property type="nucleotide sequence ID" value="XM_015182159.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VB80"/>
    <dbReference type="KEGG" id="dpo:4802161"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031985">
        <property type="term" value="C:Golgi cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000301">
        <property type="term" value="P:retrograde transport, vesicle recycling within Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019177">
        <property type="entry name" value="Golgin_subfamily_A_member_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13815:SF7">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13815">
        <property type="entry name" value="GOLGIN-84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09787">
        <property type="entry name" value="Golgin_A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="46"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="111"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359134.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015037645.2"/>
        </source>
    </evidence>
    <sequence length="513" mass="58231" checksum="B796C29C0DE761A1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDV0</accession>
    <name>A0A6I8VDV0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">sax</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">MV-25-SWS-2005</strain>
            <tissue evidence="27">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="RefSeq" id="XP_015039218.1">
        <property type="nucleotide sequence ID" value="XM_015183732.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDV0"/>
    <dbReference type="FunCoup" id="A0A6I8VDV0">
        <property type="interactions" value="456"/>
    </dbReference>
    <dbReference type="GeneID" id="4803635"/>
    <dbReference type="InParanoid" id="A0A6I8VDV0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075137">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VDV0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070724">
        <property type="term" value="C:BMP receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14142">
        <property type="entry name" value="STKc_ACVR1_ALK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23600">
        <property type="entry name" value="TFP_LU_ECD_Sax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000018">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000025">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000064">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF72">
        <property type="entry name" value="RECEPTOR PROTEIN SERINE_THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
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        <property type="entry name" value="Activin_recp"/>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Snake toxin-like"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17 27" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026240612" description="receptor protein serine/threonine kinase" evidence="23">
        <location>
            <begin position="22"/>
            <end position="578"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="25">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="272"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
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    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_015039218.1"/>
        </source>
    </evidence>
    <sequence length="578" mass="65305" checksum="0BC0AFC0541D50EE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VE06</accession>
    <name>A0A6I8VE06_DROPS</name>
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        <submittedName>
            <fullName evidence="11">Filamin-A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cher</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the filamin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_015183317.2"/>
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        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VE06"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VE06">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21311">
        <property type="entry name" value="CH_dFLNA-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21315">
        <property type="entry name" value="CH_dFLNA-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000140">
        <property type="entry name" value="FiLamiN (Actin binding protein) homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001678">
        <property type="entry name" value="filamin-A isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001682">
        <property type="entry name" value="filamin-A isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000006">
        <property type="entry name" value="Filamin-B isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000092">
        <property type="entry name" value="Filamin-B isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000008">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000007">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000001">
        <property type="entry name" value="Filamin-C isoform b"/>
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        <property type="entry name" value="filamin-C isoform X2"/>
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        <property type="entry name" value="Calponin-like domain"/>
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        <property type="entry name" value="Immunoglobulins"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
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    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF8">
        <property type="entry name" value="FILAMIN-A"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="22"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="176"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="300"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="415"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="515"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="614"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="710"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="804"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="901"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="997"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1090"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1183"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1285"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1378"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1477"/>
            <end position="1572"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1573"/>
            <end position="1667"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1720"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1778"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1868"/>
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        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="1961"/>
            <end position="2053"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2051"/>
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        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2154"/>
            <end position="2246"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2248"/>
            <end position="2341"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2343"/>
            <end position="2434"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="7">
        <location>
            <begin position="2471"/>
            <end position="2565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015038803.2"/>
        </source>
    </evidence>
    <sequence length="2565" mass="277431" checksum="A5907E34155FE7FB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VE60</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
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        <name evidence="3" type="primary">LOC26532328</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039235.1">
        <property type="nucleotide sequence ID" value="XM_015183749.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8VE60"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271315">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="entry name" value="DUF4728"/>
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        <property type="entry name" value="LP21121P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36694">
        <property type="entry name" value="PASIFLORA 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15860">
        <property type="entry name" value="DUF4728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015039235.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17339" checksum="6EC46FF17BEB9A40" modified="2020-08-12" version="1">MLLRTCCGCCSLKYGCFLVAVYTGLTYSLQLVTLLMYGLIEEYAESQIFYLFMSVPCTIAVAVSVALFVGALKKRARLLWPWLITFSVFSIIFLLTLIGSLIAGSTQSNVEDNHVGSGFMRRPLVQTLNLLMHLYVLLVVYSHYSELQNENLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VII8</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein RhoGAP18B</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">RhoGAP18B</name>
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    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
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        <property type="nucleotide sequence ID" value="XM_015186609.2"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000054">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
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    </dbReference>
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        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rho-GAP"/>
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    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
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        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF129">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 16F, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="1214"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="553"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="878"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="919"/>
            <end position="940"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="330"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="579"/>
        </location>
    </feature>
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        <location>
            <begin position="599"/>
            <end position="620"/>
        </location>
    </feature>
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        <location>
            <begin position="639"/>
            <end position="657"/>
        </location>
    </feature>
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        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
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        <location>
            <begin position="743"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="754"/>
            <end position="766"/>
        </location>
    </feature>
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        <location>
            <begin position="767"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="885"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015042095.2"/>
        </source>
    </evidence>
    <sequence length="1406" mass="156448" checksum="9074CE8575ADFE0E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VK72</accession>
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        <submittedName>
            <fullName evidence="10">IQ motif and SEC7 domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="10" type="primary">siz</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRAG family.</text>
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        <property type="nucleotide sequence ID" value="XM_015187558.2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="FunFam" id="1.10.1000.11:FF:000009">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000004">
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        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
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        <property type="entry name" value="Sec7_dom_sf"/>
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        <property type="entry name" value="FI21420P1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF16453">
        <property type="entry name" value="IQ_SEC7_PH"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
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        <property type="entry name" value="Sec7 domain"/>
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        <property type="entry name" value="IQ"/>
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        <property type="entry name" value="SEC7"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="8">
        <location>
            <begin position="793"/>
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            <end position="496"/>
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        <location>
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            <begin position="75"/>
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        <location>
            <begin position="144"/>
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        </location>
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        <location>
            <begin position="294"/>
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        </location>
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        <location>
            <begin position="405"/>
            <end position="420"/>
        </location>
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        <location>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="PROSITE" id="PS50190"/>
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    <sequence length="1363" mass="147847" checksum="BCC71E95FECD44CD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VK73</accession>
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            <dbReference type="Proteomes" id="UP000001819"/>
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            <dbReference type="RefSeq" id="XP_015042672.2"/>
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    <sequence length="691" mass="75434" checksum="F87E0FD45044642D" modified="2020-08-12" version="1"> </sequence>
</entry>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">MV2-25</strain>
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    <sequence length="352" mass="37676" checksum="0C85E7EF7E84FF04" modified="2020-08-12" version="1">MDGVKGQQSESGGETPCGLGEINMPEKMLGACSETEGGAIDVISAATTATPNVSTTDPTSHQAPEHTNQGGGVDQAFSPRTSLLDSWETFAQQRADALGRPTYATAFEGMRSIPTHSRLPQAGGLMRSTVINGGWDRAVPNWTGVHMSGPTEATRTGMPTGLNPNETMPVGAMWHPMAAPALGTNLHGQNPFIRAPTAQYYPSTSRNIPDPYACGSNQAVAPAGVERCLTASQIAARQVINKDLPCFSGNPEEWPMFIVNFEQSTERCGLRDQENLMRLQRCLKGAALEAVRGKLMMPATVRLAIETLRMLYGRPEAIYHTLQTKLRMEPSVKSSSISSAPGAVGAKLSRYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
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        </location>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232826.1"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQ52</accession>
    <name>A0A6I8VQ52_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Allatostatin-A receptor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">AstA-R2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233176.1">
        <property type="nucleotide sequence ID" value="XM_033377285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQ52"/>
    <dbReference type="FunCoup" id="A0A6I8VQ52">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VQ52"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15096">
        <property type="entry name" value="7tmA_AstA_R_insect"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000255">
        <property type="entry name" value="Allatostatin A receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF23">
        <property type="entry name" value="GALANIN-LIKE G-PROTEIN COUPLED RECEPTOR NPR-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="55"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033233176.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="44207" checksum="D6EDB8CB993449B7" modified="2020-08-12" version="1">MENITISLSNITNGSAGSVTPAPFFSEEDRKSIEDTVRWLVPFFFGIIAVTGFFGNLLVIMVVVVNKNMRSTTNLLILNLAVSDLLFVIICIPFTATDYITQRWPFGNFWCRTTQYLIVVTAFASIYTLVLMSIDRFLAVVHPIRSRMLRTEHITMIAIFTLWTVVLTISMPVTFAHDVMVHYDNTSNITYALCRFKDNDLLDASTFQLSFFISTYLLPLMIISGLYVRMITRLWRQGSGVRMSKESQRGRKRVTRLVVVVVIAFASLWLPVQLILLLKALDVYEADSLLSIIIQICAQTLAYSSSCINPLLYAFLSENFRKAFYKMVGSSWLSFLIQKPLNNAASGEHQQGENTFESITAINCSPRYQNYTSDLPPPRKTSCGRTSTTGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQS7</accession>
    <name>A0A6I8VQS7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898293</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233430.1">
        <property type="nucleotide sequence ID" value="XM_033377539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQS7"/>
    <dbReference type="KEGG" id="dpo:6898293"/>
    <dbReference type="InParanoid" id="A0A6I8VQS7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="19019" checksum="F3BFC3D32F88AC45" modified="2020-08-12" version="1">MAMQSRLIFIVLYGIFFASTLDYTDAVVFKLTNFVCESYNKSWVVFNYCRLKALFKRANGYRPWLYEIKIDMCQYFRKPFNPLVAIVYGLFKEYTNINHTCPYVGPQIVKGFYLRHELLRLPLPTGDYLVMIRWYYDKRLQFDTNVSFVFEEDLINRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQY2</accession>
    <name>A0A6I8VQY2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Odorant receptor 56a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC15382895</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033233480.1">
        <property type="nucleotide sequence ID" value="XM_033377589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQY2"/>
    <dbReference type="KEGG" id="dpo:15382895"/>
    <dbReference type="InParanoid" id="A0A6I8VQY2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF40">
        <property type="entry name" value="ODORANT RECEPTOR 56A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033233480.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="33150" checksum="00DE992C58EE720C" modified="2020-08-12" version="1">MVRAIHEDIQKLMKEADDQELVLMLSTQKYIRMATWMLWYPALLTGIIAFTDSLYRTVLLTLSVFNITERRDEQQYIFLLKVYPFGDVYNNFVFGLFGAWYALGLGINTIPLWNSFIVCLIKYVHLKLLILKKRVTEMEITRFNPLLDLDRLTPAQLNRWRMRLIKEFVKEHLKIRRFVKELEQLICLPVLIDFIFFAISICFELYALIVGHDDLCFAYYSTPWYEYDPPMKRTILFMMMHAQSPLRIRALMFPVDLKTFLDIVLGAYNYFNILRGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VRG6</accession>
    <name>A0A6I8VRG6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">BMP-binding endothelial regulator protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12 13" type="primary">cv-2</name>
    </gene>
    <organism evidence="9 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09368">
        <property type="entry name" value="LIM1_Lhx3_Lhx4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09376">
        <property type="entry name" value="LIM2_Lhx3_Lhx4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000120">
        <property type="entry name" value="Insulin gene enhancer protein ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000219">
        <property type="entry name" value="LIM/homeobox protein Lhx3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000032">
        <property type="entry name" value="LIM/homeobox protein Lhx3"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
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        <property type="entry name" value="Homeobox_CS"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR049594">
        <property type="entry name" value="Lhx3/4-like_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF128">
        <property type="entry name" value="LIM3, ISOFORM G"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="104"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="166"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="241"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="243"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="362"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="416"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="428"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="459"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033235692.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="56199" checksum="9FA89DE6F939C544" modified="2020-08-12" version="1">MHLALAMMQTLKPPPPPLHHHHPQQQLPQHLTAMDNHQQHSPHIHQPQLQQQQQQQQQQLPPTPQASHLVGSQHNHLAVDHDDPNPELVLALISRNRALEATIPKCGGCHELILDRFILKVLERTWHAKCLQCSECHGQLNDKCFARNGQLFCKEDFFKSNRRYGTKCSACDMGIPPTQVVRRAQDHVYHLQCFLCAMCSRTLNTGDEFYLMEDRKLICKRDYEEAKAKGLYLDGSLDGDQPNKRPRTTITAKQLETLKTAYNNSPKPARHVREQLSQDTGLDMRVVQVWFQNRRAKEKRLKKDAGRTRWSQYFRSMKGNCSPRTDKFLDKDELKVDYDSFSHHDLSNDSYSTVNLGLDEGASPHSIRGSYMHGSSSPSQYPPSSRSPPPVGQSHTFGSYPDNIVYTNIDHATGSTLHTSKSHHRLHSSNNVSDLSNDSSPEQGYPDFPPSPDSWLGDSASTNNTSNNNNTSNSNNNGGSGSAGASANVTNANPGTPSVHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZC6</accession>
    <name>A0A6I8VZC6_DROPS</name>
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        <recommendedName>
            <fullName evidence="4">Mitochondrial import receptor subunit TOM5 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235864.1">
        <property type="nucleotide sequence ID" value="XM_033379973.1"/>
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    <dbReference type="KEGG" id="dpo:117184023"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="45" mass="5294" checksum="6C61C8B3338291FE" modified="2020-08-12" version="1">MIRALQPDMRAVEELAKKEVQANIFVFVLTCALIRFVPKIIRKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3U9</accession>
    <name>A0A6I8W3U9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26532649</name>
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    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237374.1">
        <property type="nucleotide sequence ID" value="XM_033381483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W3U9"/>
    <dbReference type="KEGG" id="dpo:26532649"/>
    <dbReference type="InParanoid" id="A0A6I8W3U9"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033237374.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="19528" checksum="ECB16B6CF63E154E" modified="2020-08-12" version="1">MISNRHLRRLRKREREANLEKLALCREGKVIGKLEEKELAETEIQIEGKELAYENTEIQPNKSPLNDELGQCFIKQSPKRACVSPKVMGFSMPSNENEEGKTCSDVLDELKKTTETLQLENAAIRETLQLEKAAIRDQLKKVLKILADQTVLIKQLVKEKGEMNPIREQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W414</accession>
    <name>A0A6I8W414_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238081.1">
        <property type="nucleotide sequence ID" value="XM_033382190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W414"/>
    <dbReference type="KEGG" id="dpo:117184467"/>
    <dbReference type="InParanoid" id="A0A6I8W414"/>
    <dbReference type="OMA" id="PWHEHEP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081953">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W414">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238081.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15973" checksum="53DA6BB2A417C3F3" modified="2020-08-12" version="1">MGPGLGLGLGVEQEQEREQEREREPERDSIDGHNYAEIDVVNDSDFCYLTNLNNYYQEQRLNALRRGMPSLPEMKTTATSTSPWHEHEPKEDPPKSKPTPLQVLRNPKKWRVTRAVDDIVKVMIIASSEHVLDYDVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5G0</accession>
    <name>A0A6I8W5G0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Cytochrome c oxidase subunit 1</fullName>
            <ecNumber evidence="14">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC26533497</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:11437"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Mitochondrion inner membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="14"/>
    <dbReference type="RefSeq" id="XP_033238578.1">
        <property type="nucleotide sequence ID" value="XM_033382687.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26533497"/>
    <dbReference type="InParanoid" id="A0A6I8W5G0"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="11 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="16">
        <location>
            <begin position="1"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033238578.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="44956" checksum="453521F0036ED416" modified="2020-08-12" version="1">MNNIRFXLLPPALSLLLVRRIVENGAGTGXTVYPPLSSGIAHGGASVDLAIFSLHLAGISSILGAVNFITTVINIRSSGITLDRIPLFVXSVVITALLLLLSLPVLAGAITILLTDQNLNISFFDPAGGGDPILYQHLFXFFGHPEVYILILPGFGIISHIISQESGKKETFGSLGIIYAILAIGLLGFIVXAHHIFTVGIDVDTRAYFTSATIIIAVPTGIKIFSXLATLHGAQLSYSPAILXALGFVFLFTVGGLTGVVLANSSVDIILHDTYYVVAHFHYVLSMGAVFAIIAGFIHXFPLFTGLTINNKLLKSQFVTIFIGVNLTFFPQHFLGLAGIPRRYSDYPDAYTTXNVVSTIGSSISLLGILFFFYIIXESLVSQRQVIYPIQLCSSIEXYQNTPPAEHSYSELPLLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5L2</accession>
    <name>A0A6I8W5L2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Frizzled-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC4812483</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033237974.1">
        <property type="nucleotide sequence ID" value="XM_033382083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5L2"/>
    <dbReference type="FunCoup" id="A0A6I8W5L2">
        <property type="interactions" value="283"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812483"/>
    <dbReference type="InParanoid" id="A0A6I8W5L2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W5L2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15035">
        <property type="entry name" value="7tmF_FZD5_FZD8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07456">
        <property type="entry name" value="CRD_FZ5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000262">
        <property type="entry name" value="Frizzled 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000004">
        <property type="entry name" value="Frizzled class receptor 8a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF126">
        <property type="entry name" value="FRIZZLED-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026221260" evidence="17">
        <location>
            <begin position="27"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="320"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="356"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="400"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="491"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="534"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="18">
        <location>
            <begin position="67"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="19">
        <location>
            <begin position="320"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="183"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="72"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="80"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="117"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_033237974.1"/>
        </source>
    </evidence>
    <sequence length="697" mass="75339" checksum="3699690A3316D8A4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6K8</accession>
    <name>A0A6I8W6K8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4814940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238995.1">
        <property type="nucleotide sequence ID" value="XM_033383104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6K8"/>
    <dbReference type="KEGG" id="dpo:4814940"/>
    <dbReference type="InParanoid" id="A0A6I8W6K8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026334794" evidence="2">
        <location>
            <begin position="23"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033238995.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="38897" checksum="FD26F194A688E80E" modified="2020-08-12" version="1">MYLQKSLLLLVATVLSAHSIWARSAAPRQAYGYNMPLWQLQFPEKPPTSQDHPNEAPEPRLIGNVDVGFEDDLVADQQLQQLLLLSPGLSPAQFLQRYQPRSQSDVDGGRSWLGHFNGKNGGSSGGNSGGTTFVVITPGSIPNITNTNNNVINNTATNPAAATTGGTRIGEFIRSPIAYPASTYFRTRQDEASSLPYSSGLYGWNEAALYGSALDGRLGGGLDGGLAGGFSGLSTGFGSGVPLVPITIGNEVRYVPLNLRMFRQLASIPIRENEEREQDREREQEQERPIVEDLSVPMADDSEPEDLIETLTNGAGDTPSMAPPGFGRLGQRLRQRPIMRRKQLQSFAQNIRRVQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W806</accession>
    <name>A0A6I8W806_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="19 20 21">Opsin, ultraviolet-sensitive</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19 20 21" type="primary">Rh7</name>
    </gene>
    <organism evidence="18 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19 20 21">MV-25-SWS-2005</strain>
            <tissue evidence="19 20 21">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Membrane</location>
            <topology evidence="2 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134833.2">
        <property type="nucleotide sequence ID" value="XM_002134797.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042772.2">
        <property type="nucleotide sequence ID" value="XM_015187286.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239503.1">
        <property type="nucleotide sequence ID" value="XM_033383612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W806"/>
    <dbReference type="KEGG" id="dpo:6900442"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008020">
        <property type="term" value="F:G protein-coupled photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000359">
        <property type="entry name" value="opsin, ultraviolet-sensitive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0157">Chromophore</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 16" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="12 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4 16" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="14 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="331"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="137"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_002134833.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015042772.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_033239503.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="53507" checksum="9AB59153F71A1104" modified="2020-08-12" version="1">MVMEALMAALPTLTTEAAGSSLWLTSALSLSEMLANSSTSPNESLVAATTSSAAVATASTTSAAEAVGKVPDKHEVNDNVSTVLSSYPGYIHYRDKYDLSYIARVNPFWLQFEPPKSSTFYLMAALYCLISVVGCVGNAFVIFMFANRKSLRTPANILVMNLAICDFLMLVKCPIAIYNNIKEGPALGDAACRIYGFVGGLSGTCAIGTLTAIALDRYNVVVHPLQPLRRCSRLRSYLIIFLIWSYSFLFAVMPALDIGLSVYVPEGFLTTCSFDYLNKETPARIFMALFFVAAYCVPLTTIVYSYFYILKVVFTASRIQSNKDKAKTEQKLAFIVAAIIGLWFLAWSPYAIVAMMGVFGQERHITPLGSMIPALFCKTAACVDPYLYAATHPRFRVEVRMLFFGRGVLRRVSTTRSSYMTRSRSSFNRRLRPTPDAEHRVESYLMNNNLMMVPEETEENEEIVVVAEFNNSSYSGMEQSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8Q1</accession>
    <name>A0A6I8W8Q1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein Ir68a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Ir68a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239760.1">
        <property type="nucleotide sequence ID" value="XM_033383869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W8Q1"/>
    <dbReference type="FunCoup" id="A0A6I8W8Q1">
        <property type="interactions" value="8"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050907">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
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        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
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        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
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        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF35">
        <property type="entry name" value="IONOTROPIC RECEPTOR 68A, ISOFORM A"/>
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    </dbReference>
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        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026332506" evidence="10">
        <location>
            <begin position="20"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="650"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="11">
        <location>
            <begin position="437"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033239760.1"/>
        </source>
    </evidence>
    <sequence length="699" mass="79868" checksum="E3D740AA60747817" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9K5</accession>
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        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="primary">LOC6901459</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
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    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_033383393.1"/>
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        <property type="term" value="C:stereocilia ankle link complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:stereocilium tip"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="USH2_Complex_Protein"/>
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        <property type="entry name" value="HARMONIN"/>
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    </feature>
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        <location>
            <begin position="735"/>
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        <location>
            <begin position="1"/>
            <end position="34"/>
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        <location>
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        <location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033239284.1"/>
        </source>
    </evidence>
    <sequence length="773" mass="81240" checksum="50A4DDA721A42D9A" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8W9X0</accession>
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        <submittedName>
            <fullName evidence="3">uncharacterized protein LOC4815646</fullName>
        </submittedName>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_001355619.4"/>
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        <property type="nucleotide sequence ID" value="XM_033383478.1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="148"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355655.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239369.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="20702" checksum="AAA0CC3626EA7353" modified="2020-08-12" version="1">MSDNSAVTGVMNLLLCGATGFGFYQIGPQEHPYAFTACVVGFCHGLLGLVATVSGDENTTKANDATNALMEIVPLPLVNVDLFMGADSNNLALGHGLFIVPLAVSVIIGWAKDNDENGSVIDALKTLTVLGNITSMVYLALNESSWNLGGMAFLAFMAKFGAAFFEENVSEGTGQPITYISWSGFFFLTALAVAGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC117184837</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
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    <dbReference type="KEGG" id="dpo:117184837"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="entry name" value="Ng_1-3-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21827">
        <property type="entry name" value="New_glue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026317940" evidence="2">
        <location>
            <begin position="24"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239749.1"/>
        </source>
    </evidence>
    <sequence length="108" mass="11426" checksum="5248C62E84F63A44" modified="2020-08-12" version="1">MKYHCVLLLLATIGCFLVSQTSAVTATATTTSATAVTTTTVASSDTTTTTAASSDTTTTTAATSDTTTEAGSSKPKKTKRHFKRKIKRGKKKIHRRRSPKEGSESNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBL7</accession>
    <name>A0A6I8WBL7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">m7GpppN-mRNA hydrolase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">mRNA-decapping enzyme 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">DCP2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:67485"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240667.1">
        <property type="nucleotide sequence ID" value="XM_033384776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WBL7"/>
    <dbReference type="FunCoup" id="A0A6I8WBL7">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813367"/>
    <dbReference type="InParanoid" id="A0A6I8WBL7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WBL7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1050:FF:000001">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000003">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="18">
        <location>
            <begin position="348"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="175"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="499"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="584"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="741"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="791"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="593"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="757"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="793"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033240667.1"/>
        </source>
    </evidence>
    <sequence length="845" mass="93828" checksum="5B67D6C46AFD1917" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBP8</accession>
    <accession>Q29J93</accession>
    <name>A0A6I8WBP8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-actinin, sarcomeric</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">F-actin cross-linking protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 14 15" type="primary">Actn</name>
        <name evidence="13" type="synonym">LOC4815437</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14 15">MV-25-SWS-2005</strain>
            <strain evidence="13">MV2-25</strain>
            <tissue evidence="13 14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-actinin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355351.3">
        <property type="nucleotide sequence ID" value="XM_001355315.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240817.1">
        <property type="nucleotide sequence ID" value="XM_033384926.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240818.1">
        <property type="nucleotide sequence ID" value="XM_033384927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WBP8"/>
    <dbReference type="SMR" id="A0A6I8WBP8"/>
    <dbReference type="GeneID" id="4815437"/>
    <dbReference type="KEGG" id="dpo:4815437"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078151">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000005">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000148">
        <property type="entry name" value="alpha-actinin, sarcomeric isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="34"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="147"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="771"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="812"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001355351.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033240817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033240818.1"/>
        </source>
    </evidence>
    <sequence length="917" mass="106377" checksum="86C9B47A9CC322DD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBU6</accession>
    <name>A0A6I8WBU6_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">CASC1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6900381</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240876.1">
        <property type="nucleotide sequence ID" value="XM_033384985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WBU6"/>
    <dbReference type="KEGG" id="dpo:6900381"/>
    <dbReference type="InParanoid" id="A0A6I8WBU6"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CASC1_C"/>
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        <property type="entry name" value="IC97/Dnai7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20929:SF11">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20929">
        <property type="entry name" value="LUNG ADENOMA SUSCEPTIBILITY 1-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF12366">
        <property type="entry name" value="Casc1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="779"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="760"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033240876.1"/>
        </source>
    </evidence>
    <sequence length="1048" mass="121691" checksum="6EEC5985B4C5A365" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBX5</accession>
    <name>A0A6I8WBX5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein rogdi</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rogdi</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the rogdi family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240767.1">
        <property type="nucleotide sequence ID" value="XM_033384876.1"/>
    </dbReference>
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        <property type="interactions" value="577"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028241">
        <property type="entry name" value="RAVE2/Rogdi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618">
        <property type="entry name" value="LEUCINE ZIPPER CONTAINING TRANSCRIPTION FACTOR LZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13618:SF1">
        <property type="entry name" value="PROTEIN ROGDI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10259">
        <property type="entry name" value="Rogdi_lz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240767.1"/>
        </source>
    </evidence>
    <sequence length="339" mass="38524" checksum="8553781590920889" modified="2020-08-12" version="1">MPQSPTTNWSFQPLHRQRSGSGYHQHVLNTVPETAMLTTPPPIRKPLTIQIAGVAENKRRYIPARKKPKSAMKMLVDTEREEALNLQIEFEWVLRQEVHAILKQLRTILVECAHRFPVPLYENEGKKTEKFILTVSPDQLKAVLTLTGDAITQADISFKLCKAPSQTQRTSITQDSPWKLQQVQDAANHLQTAINHIDDVDDSYHFKTSDEVLHVIGNILDALQRGRNSLLVPKKKPIDELIKGRNMKSLVPNLPEDLAVSFYLQSHKLIIAVYQLLNNQGTMRFDSRQAEASVQWLNDVLLLLMNGQKLCQQLKDKISVFSVYKDFTVGSRSPSALSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCA6</accession>
    <name>A0A6I8WCA6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small nuclear ribonucleoprotein-associated protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901769</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240194.1">
        <property type="nucleotide sequence ID" value="XM_033384303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCA6"/>
    <dbReference type="KEGG" id="dpo:6901769"/>
    <dbReference type="InParanoid" id="A0A6I8WCA6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026307512" evidence="2">
        <location>
            <begin position="24"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033240194.1"/>
        </source>
    </evidence>
    <sequence length="67" mass="6521" checksum="F127D06C3769DD9C" modified="2020-08-12" version="1">MHFNCHYLISLLLMLLCGSLVSAYPQGGPCGPPPSGPPPSGPPPSGRPPGGPGGRCGPPPSTTASSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WE44</accession>
    <name>A0A6I8WE44_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4801044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241706.1">
        <property type="nucleotide sequence ID" value="XM_033385815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WE44"/>
    <dbReference type="FunCoup" id="A0A6I8WE44">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801044"/>
    <dbReference type="InParanoid" id="A0A6I8WE44"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WE44">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46560">
        <property type="entry name" value="CYPHER, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46560:SF9">
        <property type="entry name" value="ZP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026290868" evidence="2">
        <location>
            <begin position="28"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="3">
        <location>
            <begin position="44"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033241706.1"/>
        </source>
    </evidence>
    <sequence length="437" mass="47673" checksum="40281B0EB7CA8BFE" modified="2020-08-12" version="1">MIQRPKTVGPGLLLLPLILLLAGQAATQEPAASPGEFAPHVTATCKAGTMNIKVKVNSGYTGAVHARDFRTPACMAMGDGSDAVAFSLNLWAKQGASDYCGILVSNVSGSNRTEERSIQLAVRVHKTLELADDKFYVITCGKSGYTRDDNAHVVLKFLENDHRVRETVYGHEYKIRAEFSKPNDTYGLRVGNCFAFDKKNRTLKLTDDSGCPYDATIISRFVPTADGRAAEATLTSMFKFPEGSEVHLQCDVIQCYGRCVEIDDCNDVALAGFTKGSNGPRKFGPNEEGSSLAGTTVFVLDPAEARLVSSSCEDGIRPSWLLWLTITLGVLFLIMLLMNIFLCTAMSCSCANTEIIEKEPSIIEEYDPYRSWHGSQYGSRYSLHGRDAHKGYTSGGSTIHSNRSIPIDNDNDYAIVHSRPGSTIHGQRAHRGPPSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8H0U8</accession>
    <name>A0ABD8H0U8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Gmppb</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001359425.1">
        <property type="nucleotide sequence ID" value="XM_001359388.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8H0U8"/>
    <dbReference type="SMR" id="A0ABD8H0U8"/>
    <dbReference type="GeneID" id="4802517"/>
    <dbReference type="KEGG" id="dpo:4802517"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000018">
        <property type="entry name" value="Mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000013">
        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="10">
        <location>
            <begin position="15"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="11">
        <location>
            <begin position="261"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359425.1"/>
        </source>
    </evidence>
    <sequence length="371" mass="40603" checksum="FFB52C75F3AC9616" modified="2025-06-18" version="1">MCGSPNTTVGNGTRALILVGGYGTRLRPLTLSTPKPLVEFANKPILLHQLEALVDAGCRQVILAVSYRAEQMEKELKVEADKLGVELIFSHETEPLGTAGPLALAKSILSASPEPFFVLNSDVICDFPFKQLVQFHRNHGKEGTIVVTKVEEPSKYGVVLYDEDGCIKNFIEKPQEFVSNKINAGIYIFNPSVLERIEVKPTSIEKEVFPAMAEQQELYAMDLTGFWMDIGQPKDFLTGMCLYLSSLRQKQSPKLYTGPGVVGNVLVDPTATIGEGCRIGPNVTIGPDVIIEDGVCIKRATILKGAIVRSHSWLDSCIVGWRSTVGRWVRIEGITVLGEDVIVKDELYVNGGQVLPHKSIAASVPEPQIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>B5DL77</accession>
    <accession>A0A6I8UYP6</accession>
    <name>B5DL77_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">28S ribosomal protein S14, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mRpS14</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133649.1">
        <property type="nucleotide sequence ID" value="XM_002133613.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DL77"/>
    <dbReference type="SMR" id="B5DL77"/>
    <dbReference type="FunCoup" id="B5DL77">
        <property type="interactions" value="628"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DL77"/>
    <dbReference type="GeneID" id="6902118"/>
    <dbReference type="KEGG" id="dpo:6902118"/>
    <dbReference type="eggNOG" id="KOG1741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139869_1_0_1"/>
    <dbReference type="InParanoid" id="B5DL77"/>
    <dbReference type="OMA" id="FGLCRNQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244099">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DL77">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1480:FF:000001">
        <property type="entry name" value="30S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836:SF19">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00083755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002133649.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="14859" checksum="63E4E147DC76C7FE" modified="2008-10-14" version="1">MNSLARIGGFACQAGKLAGFGLQQVRTKYADWKMIRDVKRRKCVQEHAKERLRVNSLRKNDILPIELREVADAEIAAFPRDSSLVRVRERCALTSRPRGVVHKYRLSRIVWRHLADYNKLSGVQRAMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>B5DS00</accession>
    <accession>A0A6I8V2L2</accession>
    <name>B5DS00_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fibulin-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6899872</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135665.2">
        <property type="nucleotide sequence ID" value="XM_002135629.3"/>
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    <dbReference type="AlphaFoldDB" id="B5DS00"/>
    <dbReference type="FunCoup" id="B5DS00">
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    <dbReference type="STRING" id="46245.B5DS00"/>
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    <dbReference type="KEGG" id="dpo:6899872"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004826_1_0_1"/>
    <dbReference type="InParanoid" id="B5DS00"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="2"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
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    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
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    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055088">
        <property type="entry name" value="Fibulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF28">
        <property type="entry name" value="UROMODULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22914">
        <property type="entry name" value="Fibulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="352"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="585"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="631"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="679"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="720"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002135665.2"/>
        </source>
    </evidence>
    <sequence length="938" mass="104880" checksum="E00A650D26FD7311" modified="2016-01-20" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>B5DT50</accession>
    <accession>A0A6I8V2P6</accession>
    <name>B5DT50_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">carboxylesterase</fullName>
            <ecNumber evidence="6">3.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Dpse\GA25484</name>
        <name evidence="11" type="synonym">LOC6899467</name>
        <name evidence="9" type="ORF">Dpse_GA25484</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
                <person name="Castrezana S."/>
                <person name="Celniker S.E."/>
                <person name="Chang J.L."/>
                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
                <person name="Clifton S.W."/>
                <person name="Comeron J.M."/>
                <person name="Costello J.C."/>
                <person name="Coyne J.A."/>
                <person name="Daub J."/>
                <person name="David R.G."/>
                <person name="Delcher A.L."/>
                <person name="Delehaunty K."/>
                <person name="Do C.B."/>
                <person name="Ebling H."/>
                <person name="Edwards K."/>
                <person name="Eickbush T."/>
                <person name="Evans J.D."/>
                <person name="Filipski A."/>
                <person name="Findeiss S."/>
                <person name="Freyhult E."/>
                <person name="Fulton L."/>
                <person name="Fulton R."/>
                <person name="Garcia A.C."/>
                <person name="Gardiner A."/>
                <person name="Garfield D.A."/>
                <person name="Garvin B.E."/>
                <person name="Gibson G."/>
                <person name="Gilbert D."/>
                <person name="Gnerre S."/>
                <person name="Godfrey J."/>
                <person name="Good R."/>
                <person name="Gotea V."/>
                <person name="Gravely B."/>
                <person name="Greenberg A.J."/>
                <person name="Griffiths-Jones S."/>
                <person name="Gross S."/>
                <person name="Guigo R."/>
                <person name="Gustafson E.A."/>
                <person name="Haerty W."/>
                <person name="Hahn M.W."/>
                <person name="Halligan D.L."/>
                <person name="Halpern A.L."/>
                <person name="Halter G.M."/>
                <person name="Han M.V."/>
                <person name="Heger A."/>
                <person name="Hillier L."/>
                <person name="Hinrichs A.S."/>
                <person name="Holmes I."/>
                <person name="Hoskins R.A."/>
                <person name="Hubisz M.J."/>
                <person name="Hultmark D."/>
                <person name="Huntley M.A."/>
                <person name="Jaffe D.B."/>
                <person name="Jagadeeshan S."/>
                <person name="Jeck W.R."/>
                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
                <person name="Li R."/>
                <person name="Lin C.F."/>
                <person name="Lin M.F."/>
                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
                <person name="Machado C.A."/>
                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
                <person name="O'Grady P."/>
                <person name="Pachter L."/>
                <person name="Papaceit M."/>
                <person name="Parisi M.J."/>
                <person name="Parisi M."/>
                <person name="Parts L."/>
                <person name="Pedersen J.S."/>
                <person name="Pesole G."/>
                <person name="Phillippy A.M."/>
                <person name="Ponting C.P."/>
                <person name="Pop M."/>
                <person name="Porcelli D."/>
                <person name="Powell J.R."/>
                <person name="Prohaska S."/>
                <person name="Pruitt K."/>
                <person name="Puig M."/>
                <person name="Quesneville H."/>
                <person name="Ram K.R."/>
                <person name="Rand D."/>
                <person name="Rasmussen M.D."/>
                <person name="Reed L.K."/>
                <person name="Reenan R."/>
                <person name="Reily A."/>
                <person name="Remington K.A."/>
                <person name="Rieger T.T."/>
                <person name="Ritchie M.G."/>
                <person name="Robin C."/>
                <person name="Rogers Y.H."/>
                <person name="Rohde C."/>
                <person name="Rozas J."/>
                <person name="Rubenfield M.J."/>
                <person name="Ruiz A."/>
                <person name="Russo S."/>
                <person name="Salzberg S.L."/>
                <person name="Sanchez-Gracia A."/>
                <person name="Saranga D.J."/>
                <person name="Sato H."/>
                <person name="Schaeffer S.W."/>
                <person name="Schatz M.C."/>
                <person name="Schlenke T."/>
                <person name="Schwartz R."/>
                <person name="Segarra C."/>
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                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
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                <person name="Stage D.E."/>
                <person name="Stark A."/>
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                <person name="Sutton G.G."/>
                <person name="Tao W."/>
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                <person name="Tobari Y.N."/>
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                <person name="Tsolas J.M."/>
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                <person name="Villasante A."/>
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                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
                <person name="Costello M."/>
                <person name="D'Aco K."/>
                <person name="Daza R."/>
                <person name="De Haan G."/>
                <person name="DeGray S."/>
                <person name="DeMaso C."/>
                <person name="Dhargay N."/>
                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
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                <person name="Dupes A."/>
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                <person name="Farina A."/>
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                <person name="Ferguson D."/>
                <person name="Fisher S."/>
                <person name="Foley C.D."/>
                <person name="Franke A."/>
                <person name="Friedrich D."/>
                <person name="Gadbois L."/>
                <person name="Gearin G."/>
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                <person name="Giannoukos G."/>
                <person name="Goode T."/>
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                <person name="Grewal S."/>
                <person name="Gyaltsen K."/>
                <person name="Hafez N."/>
                <person name="Hagos B."/>
                <person name="Hall J."/>
                <person name="Henson C."/>
                <person name="Hollinger A."/>
                <person name="Honan T."/>
                <person name="Huard M.D."/>
                <person name="Hughes L."/>
                <person name="Hurhula B."/>
                <person name="Husby M.E."/>
                <person name="Kamat A."/>
                <person name="Kanga B."/>
                <person name="Kashin S."/>
                <person name="Khazanovich D."/>
                <person name="Kisner P."/>
                <person name="Lance K."/>
                <person name="Lara M."/>
                <person name="Lee W."/>
                <person name="Lennon N."/>
                <person name="Letendre F."/>
                <person name="LeVine R."/>
                <person name="Lipovsky A."/>
                <person name="Liu X."/>
                <person name="Liu J."/>
                <person name="Liu S."/>
                <person name="Lokyitsang T."/>
                <person name="Lokyitsang Y."/>
                <person name="Lubonja R."/>
                <person name="Lui A."/>
                <person name="MacDonald P."/>
                <person name="Magnisalis V."/>
                <person name="Maru K."/>
                <person name="Matthews C."/>
                <person name="McCusker W."/>
                <person name="McDonough S."/>
                <person name="Mehta T."/>
                <person name="Meldrim J."/>
                <person name="Meneus L."/>
                <person name="Mihai O."/>
                <person name="Mihalev A."/>
                <person name="Mihova T."/>
                <person name="Mittelman R."/>
                <person name="Mlenga V."/>
                <person name="Montmayeur A."/>
                <person name="Mulrain L."/>
                <person name="Navidi A."/>
                <person name="Naylor J."/>
                <person name="Negash T."/>
                <person name="Nguyen T."/>
                <person name="Nguyen N."/>
                <person name="Nicol R."/>
                <person name="Norbu C."/>
                <person name="Norbu N."/>
                <person name="Novod N."/>
                <person name="O'Neill B."/>
                <person name="Osman S."/>
                <person name="Markiewicz E."/>
                <person name="Oyono O.L."/>
                <person name="Patti C."/>
                <person name="Phunkhang P."/>
                <person name="Pierre F."/>
                <person name="Priest M."/>
                <person name="Raghuraman S."/>
                <person name="Rege F."/>
                <person name="Reyes R."/>
                <person name="Rise C."/>
                <person name="Rogov P."/>
                <person name="Ross K."/>
                <person name="Ryan E."/>
                <person name="Settipalli S."/>
                <person name="Shea T."/>
                <person name="Sherpa N."/>
                <person name="Shi L."/>
                <person name="Shih D."/>
                <person name="Sparrow T."/>
                <person name="Spaulding J."/>
                <person name="Stalker J."/>
                <person name="Stange-Thomann N."/>
                <person name="Stavropoulos S."/>
                <person name="Stone C."/>
                <person name="Strader C."/>
                <person name="Tesfaye S."/>
                <person name="Thomson T."/>
                <person name="Thoulutsang Y."/>
                <person name="Thoulutsang D."/>
                <person name="Topham K."/>
                <person name="Topping I."/>
                <person name="Tsamla T."/>
                <person name="Vassiliev H."/>
                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
                <person name="Weiand M."/>
                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
                <person name="Yu Q."/>
                <person name="Zembek L."/>
                <person name="Zhong D."/>
                <person name="Zimmer A."/>
                <person name="Zwirko Z."/>
                <person name="Jaffe D.B."/>
                <person name="Alvarez P."/>
                <person name="Brockman W."/>
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                <person name="Chin C."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
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                <person name="Mauceli E."/>
                <person name="MacCallum I."/>
            </authorList>
            <dbReference type="PubMed" id="17994087"/>
            <dbReference type="DOI" id="10.1038/nature06341"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
            <authorList>
                <person name="English A.C."/>
                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
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                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="5">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="6">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.1" evidence="6"/>
    <dbReference type="EMBL" id="CH475728">
        <property type="protein sequence ID" value="EDY71024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002136065.1">
        <property type="nucleotide sequence ID" value="XM_002136029.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DT50"/>
    <dbReference type="STRING" id="46245.B5DT50"/>
    <dbReference type="ESTHER" id="drops-q294n7">
        <property type="family name" value="Carb_B_Arthropoda"/>
    </dbReference>
    <dbReference type="GeneID" id="6899467"/>
    <dbReference type="KEGG" id="dpo:6899467"/>
    <dbReference type="eggNOG" id="KOG1516">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2388548_0_0_1"/>
    <dbReference type="OMA" id="AGAISCH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246862">
        <property type="expression patterns" value="Expressed in male reproductive system"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DT50">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="8">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EDY71024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002136065.1"/>
        </source>
    </evidence>
    <sequence length="94" mass="10408" checksum="B27501FDA72CFA2E" modified="2008-10-14" version="1">MRENVIHISIGHRLGPLGFLSFADPSLEIPGNAGLKDLILALKWIKANARNFNGDPERITLFGHSSGSMAVLKKIYFHLLLPVFYPLNTILIAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-05-01" modified="2025-06-18" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>P25725</accession>
    <accession>O16180</accession>
    <accession>O16181</accession>
    <accession>O16182</accession>
    <accession>O16183</accession>
    <accession>O16184</accession>
    <accession>O16185</accession>
    <accession>O16186</accession>
    <name>EST5C_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Esterase-5C</fullName>
            <shortName>Est-5C</shortName>
            <ecNumber>3.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Carboxylic-ester hydrolase 5C</fullName>
            <shortName>Carboxylesterase-5C</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">Est-5C</name>
        <name type="synonym">Est5C</name>
        <name type="ORF">GA19955</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1990" name="Mol. Biol. Evol." volume="7" first="525" last="546">
            <title>Cloning of the esterase-5 locus from Drosophila pseudoobscura and comparison with its homologue in D. melanogaster.</title>
            <authorList>
                <person name="Brady J.P."/>
                <person name="Richmond R.C."/>
                <person name="Oakeshott J.G."/>
            </authorList>
            <dbReference type="PubMed" id="2178209"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Genetics" volume="148" first="305" last="315">
            <title>The role of gene conversion in determining sequence variation and divergence in the Est-5 gene family in Drosophila pseudoobscura.</title>
            <authorList>
                <person name="King L.M."/>
            </authorList>
            <dbReference type="PubMed" id="9475741"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>GB115E</strain>
            <strain>GB139E</strain>
            <strain>GB336E</strain>
            <strain>GB4E</strain>
            <strain>GB8E</strain>
            <strain>JR198E</strain>
            <strain>JR341E</strain>
            <strain>JR50E</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a carboxylic ester + H2O = an alcohol + a carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:33308"/>
            <dbReference type="EC" id="3.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.1"/>
    <dbReference type="EMBL" id="M55907">
        <property type="protein sequence ID" value="AAA28514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016143">
        <property type="protein sequence ID" value="AAB70233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016144">
        <property type="protein sequence ID" value="AAB70234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016145">
        <property type="protein sequence ID" value="AAB70235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016146">
        <property type="protein sequence ID" value="AAB70236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016147">
        <property type="protein sequence ID" value="AAB70237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016148">
        <property type="protein sequence ID" value="AAB70238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016149">
        <property type="protein sequence ID" value="AAB70239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF016150">
        <property type="protein sequence ID" value="AAB70240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CH379069">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P25725"/>
    <dbReference type="SMR" id="P25725"/>
    <dbReference type="FunCoup" id="P25725">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="46245.P25725"/>
    <dbReference type="ESTHER" id="drops-est5c">
        <property type="family name" value="Carb_B_Arthropoda"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.947"/>
    <dbReference type="GlyCosmos" id="P25725">
        <property type="glycosylation" value="3 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1516">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="P25725"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00312">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000378">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0719">Serine esterase</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000008560" description="Esterase-5C">
        <location>
            <begin position="20"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="3">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="467"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="507"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB8E.">
        <original>L</original>
        <variation>R</variation>
        <location>
            <position position="6"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB4E.">
        <original>S</original>
        <variation>R</variation>
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB8E and GB336E.">
        <original>F</original>
        <variation>N</variation>
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR50E and GB139E.">
        <original>E</original>
        <variation>D</variation>
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR50E, GB8E and GB115E.">
        <original>V</original>
        <variation>A</variation>
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR341E.">
        <original>F</original>
        <variation>S</variation>
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB4E, JR50E, GB115E, GB139E, GB336E and JR341E.">
        <original>D</original>
        <variation>G</variation>
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB4E, JR50E, GB115E, GB139E, GB336E and JR341E.">
        <original>M</original>
        <variation>T</variation>
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR50E.">
        <original>A</original>
        <variation>G</variation>
        <location>
            <position position="291"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB115E.">
        <original>A</original>
        <variation>T</variation>
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: GB336E.">
        <original>V</original>
        <variation>I</variation>
        <location>
            <position position="461"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: JR50E and JR341E.">
        <original>E</original>
        <variation>V</variation>
        <location>
            <position position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="545" mass="61021" checksum="60535F4DFA639D6A" modified="1992-05-01" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-08-21" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q291H5</accession>
    <name>CDKAL_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Threonylcarbamoyladenosine tRNA methylthiotransferase</fullName>
            <ecNumber evidence="1">2.8.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>CDKAL1-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA-t(6)A37 methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GA19679</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="1"/>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL25137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q291H5"/>
    <dbReference type="SMR" id="Q291H5"/>
    <dbReference type="FunCoup" id="Q291H5">
        <property type="interactions" value="2158"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q291H5"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0278387">
        <property type="protein sequence ID" value="FBpp0276825"/>
        <property type="gene ID" value="FBgn0079675"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803921"/>
    <dbReference type="eggNOG" id="KOG4355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_4_1_1"/>
    <dbReference type="InParanoid" id="Q291H5"/>
    <dbReference type="OMA" id="HYAYPTG"/>
    <dbReference type="PhylomeDB" id="Q291H5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079675">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000005">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000002">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000298677" description="Threonylcarbamoyladenosine tRNA methylthiotransferase">
        <location>
            <begin position="1"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="4">
        <location>
            <begin position="72"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="207"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="3">
        <location>
            <begin position="438"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q91WE6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <sequence length="553" mass="62720" checksum="62680891300F7A89" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CJ5</accession>
    <accession>A0A6I8UA89</accession>
    <name>Q29CJ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cullin-associated NEDD8-dissociated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Cand1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAND family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352264.1">
        <property type="nucleotide sequence ID" value="XM_001352228.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29CJ5"/>
    <dbReference type="FunCoup" id="Q29CJ5">
        <property type="interactions" value="2146"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29CJ5"/>
    <dbReference type="GeneID" id="4811679"/>
    <dbReference type="KEGG" id="dpo:4811679"/>
    <dbReference type="eggNOG" id="KOG1824">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007157_0_0_1"/>
    <dbReference type="InParanoid" id="Q29CJ5"/>
    <dbReference type="OMA" id="AYIPHFQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078827">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010265">
        <property type="term" value="P:SCF complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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        <property type="entry name" value="CAND1/CAND2"/>
    </dbReference>
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        <property type="entry name" value="TATA-bd_TIP120"/>
    </dbReference>
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        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25782">
        <property type="entry name" value="TPR_CAND1"/>
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    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="TATA-binding protein interacting (TIP20)" evidence="4">
        <location>
            <begin position="1056"/>
            <end position="1218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352264.1"/>
        </source>
    </evidence>
    <sequence length="1248" mass="139632" checksum="5889DBE1246D91A1" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CZ0</accession>
    <accession>A0A6I8UC50</accession>
    <name>Q29CZ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MORN repeat-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4812744</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353122.2">
        <property type="nucleotide sequence ID" value="XM_001353086.4"/>
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    <dbReference type="AlphaFoldDB" id="Q29CZ0"/>
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    <dbReference type="GeneID" id="4812744"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084314_0_0_1"/>
    <dbReference type="InParanoid" id="Q29CZ0"/>
    <dbReference type="OMA" id="YPIRRSK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077267">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29CZ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042814">
        <property type="entry name" value="Morn5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46437">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46437:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="241"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="284"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353122.2"/>
        </source>
    </evidence>
    <sequence length="313" mass="35545" checksum="6D8AFCCFF0B44971" modified="2008-10-14" version="2">MERYTTLVRDSTGEGRSIDGTRNSNFPTGTKFFGQMNELGMQDYGTYTYPDGSRYVGYFRNNHFHGSGSIQLPAPLGVSFQVTHHDGKLLKIDEMVFDDQLKVDFARMKDGSVSFHPWKYCSSEDRRFFTETRSPLAAVGPYKYQTQDGPSPPDLPLNVFDLGFGRLHPQGYLMDLKHGSPSIYVGCRKVRHWIMENCRHGKLTKYHLRSAVKAKFDRQIMKNNLEVNQICQQTSGGVPPIPIPGVCHRSKSQQSSESAKETRLHAASTSDSCSSKIDRTVKKEPRRKCRKTPTKGRREKRSDTSETHACRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E64</accession>
    <accession>A0A6I8UB42</accession>
    <name>Q29E64_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4811938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352699.2">
        <property type="nucleotide sequence ID" value="XM_001352663.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E64"/>
    <dbReference type="FunCoup" id="Q29E64">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E64"/>
    <dbReference type="GeneID" id="4811938"/>
    <dbReference type="KEGG" id="dpo:4811938"/>
    <dbReference type="eggNOG" id="ENOG502S0QK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_998437_0_0_1"/>
    <dbReference type="InParanoid" id="Q29E64"/>
    <dbReference type="OMA" id="LFCSMLM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072222">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031720">
        <property type="entry name" value="DUF4728"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36694:SF11">
        <property type="entry name" value="LP21121P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36694">
        <property type="entry name" value="PASIFLORA 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15860">
        <property type="entry name" value="DUF4728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352699.2"/>
        </source>
    </evidence>
    <sequence length="297" mass="33625" checksum="42CDA4CE2C2D9819" modified="2008-10-14" version="2">MAILESCCFWKNVRMGSFACAIYTLIYFGFSTLMFLFYLREEQEFLLGQRSEPAGESMLEKGDVTVVTVIFNVFFLFCSVLMVLSSGLMIVGLRRNKRQLLIPWICFMLADLLIECSHLVHLTLSRRVIFDPIVGFIFTIDFFILCLNLYCLLCVISQYQAYRDQRSEQQENPTTSPVVVFTAAEKLSKTQQQHQQQQLQHQAQLLGDGSGNGKRQSRRMLGAASPLITSQRLTNFSTITEEEEQQSRAGHSNGHLSDQPGSSVAEPELDMDSGNGELQVTKQIITLDSVEMAEIQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E72</accession>
    <accession>A0A6I8UB38</accession>
    <name>Q29E72_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Signal recognition particle 19 kDa protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Srp19</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). Binds directly to 7SL RNA. Mediates binding of SRP54 to the SRP complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SRP19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352691.2">
        <property type="nucleotide sequence ID" value="XM_001352655.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E72"/>
    <dbReference type="FunCoup" id="Q29E72">
        <property type="interactions" value="1383"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E72"/>
    <dbReference type="GeneID" id="4812254"/>
    <dbReference type="KEGG" id="dpo:4812254"/>
    <dbReference type="eggNOG" id="KOG3198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064201_2_1_1"/>
    <dbReference type="InParanoid" id="Q29E72"/>
    <dbReference type="OMA" id="QMERWIC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078205">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29E72">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006617">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.30:FF:000002">
        <property type="entry name" value="Signal recognition particle 19kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="123"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001352691.2"/>
        </source>
    </evidence>
    <sequence length="169" mass="18896" checksum="2F91A1939560ECD3" modified="2008-10-14" version="2">MAEPLPMRINWNPDLKHNQMERWICIYPAYINSKKTRQEGRRLPKENCVENPHYHEMRDVLSVTNMHFLVETLKYPREISKEAQFRGRIRVQLRNADGTLCSKDFPSRESIMQYIAGKIPQLKTRQNKSADSFHQPQLQSSGSGASGGGSGGGGGGGGGGGKKGKGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EG7</accession>
    <accession>A0A6I8UAU3</accession>
    <name>Q29EG7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proteasome subunit beta type-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Prosbeta6</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352596.1">
        <property type="nucleotide sequence ID" value="XM_001352560.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EG7"/>
    <dbReference type="SMR" id="Q29EG7"/>
    <dbReference type="FunCoup" id="Q29EG7">
        <property type="interactions" value="1738"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EG7"/>
    <dbReference type="MEROPS" id="T01.986"/>
    <dbReference type="GeneID" id="4812557"/>
    <dbReference type="KEGG" id="dpo:4812557"/>
    <dbReference type="eggNOG" id="KOG0179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_1_1_1"/>
    <dbReference type="InParanoid" id="Q29EG7"/>
    <dbReference type="OMA" id="CSGCWCD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077964">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03757">
        <property type="entry name" value="proteasome_beta_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000027">
        <property type="entry name" value="Proteasome subunit beta type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001352596.1"/>
        </source>
    </evidence>
    <sequence length="235" mass="25840" checksum="191E6AB493E930B0" modified="2006-04-04" version="1">MSNLGIEQFPDYQVPGVSHADFSPYESNGGSIVAIAGEDFVVIAADTRLSSGYNIHTRKQSKLFPLSNQTVLASTGCWCDTLSLTGLMKLRMQNYEHTHLKTMTTDAVAQMLSIIMYNRRFFPYYVSNILAGIDKDGKGVVFSYDPIGHCERATYRAGGTAGALLQPVLDNQIGYKNMNLHGEDAPEVTKERAVAVASDTFISAAERDIYTGDSVLINIITKDGIETQELQLRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EW0</accession>
    <accession>A0A6I8UAI8</accession>
    <name>Q29EW0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipid droplet-associated hydrolase</fullName>
            <ecNumber evidence="9">3.1.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Lipid droplet-associated serine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">sturkopf</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a cholesterol ester + H2O = cholesterol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36403"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17002"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:36404"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. LDAH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.13" evidence="9"/>
    <dbReference type="RefSeq" id="XP_001352453.2">
        <property type="nucleotide sequence ID" value="XM_001352417.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EW0"/>
    <dbReference type="FunCoup" id="Q29EW0">
        <property type="interactions" value="1557"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EW0"/>
    <dbReference type="ESTHER" id="drops-q29ew0">
        <property type="family name" value="LIDHydrolase"/>
    </dbReference>
    <dbReference type="GeneID" id="4812436"/>
    <dbReference type="KEGG" id="dpo:4812436"/>
    <dbReference type="eggNOG" id="KOG3975">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018394_2_1_1"/>
    <dbReference type="InParanoid" id="Q29EW0"/>
    <dbReference type="OMA" id="WVPVSYY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081584">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EW0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000068">
        <property type="entry name" value="Lipid droplet associated hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019363">
        <property type="entry name" value="LDAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390:SF0">
        <property type="entry name" value="LIPID DROPLET-ASSOCIATED HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10230">
        <property type="entry name" value="LIDHydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001352453.2"/>
        </source>
    </evidence>
    <sequence length="307" mass="35544" checksum="22DD75CC7ED76D65" modified="2008-10-14" version="2">MQEAYVNINSIPTHILTWGRWIEETVTEKELVICITGNPGLPGFYTEFASTLQKQLGDLPVWVIGHAGHDDPPEASIREVPQLTGNEEIFNLDGQIKHKLAFIKKYVPKDVKIHLIGHSIGAWMILQMLEDESVRSRVQKCYMLFPTVERMIESPNGWVFTKLAMPLYSVFGYVFFKFFNALPVWLRLFLIQIYFLIFSIPRSFLDTALQYSKPSVAEKVVFLADDEMCRVRGLQKEIIERNIHLLKFYYGTTDGWVPVSYYEQLKKDYPQVDATLDGQKIDHAFVLRHSQPVAAIVREMIQQNLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q29G27</accession>
    <accession>A0A6I8UET1</accession>
    <name>Q29G27_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein upd1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">upd1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354349.1">
        <property type="nucleotide sequence ID" value="XM_001354313.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29G27"/>
    <dbReference type="FunCoup" id="Q29G27">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29G27"/>
    <dbReference type="GeneID" id="4814412"/>
    <dbReference type="KEGG" id="dpo:4814412"/>
    <dbReference type="eggNOG" id="ENOG502T8AU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037797_0_0_1"/>
    <dbReference type="InParanoid" id="Q29G27"/>
    <dbReference type="OMA" id="MDMRFVK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079280">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001700">
        <property type="term" value="P:embryonic development via the syncytial blastoderm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031901">
        <property type="entry name" value="Unpaired"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15972">
        <property type="entry name" value="Unpaired"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="505"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="523"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354349.1"/>
        </source>
    </evidence>
    <sequence length="539" mass="60930" checksum="A45A7134029A3E3E" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HC3</accession>
    <accession>A0A6I8UFQ1</accession>
    <name>Q29HC3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4815240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354780.2">
        <property type="nucleotide sequence ID" value="XM_001354744.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HC3"/>
    <dbReference type="STRING" id="46245.Q29HC3"/>
    <dbReference type="GeneID" id="4815240"/>
    <dbReference type="KEGG" id="dpo:4815240"/>
    <dbReference type="eggNOG" id="ENOG502RTM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1355911_0_0_1"/>
    <dbReference type="InParanoid" id="Q29HC3"/>
    <dbReference type="OMA" id="PELPKQM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071701">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HC3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001354780.2"/>
        </source>
    </evidence>
    <sequence length="200" mass="22378" checksum="DD73BA65163CF92E" modified="2008-10-14" version="2">MGKKGKGKKGKKGKKGKKSIAVAPVAAEPCPPCPEIPKAMAPLDPCPETSGPHDVARAQPNHYPALLRIPETMAVVGSENGCYDSICKLLRAGFRCAERVFVMAPWGNYVVFRYHNNSDFIYFLFDGCTCDVNRFRYLDLSCGTAGFLYFDNMHDVISYIIQSRKTRLYLENLNKIAIDQIVEEYGAVTYTQKAKCMRFA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-03-02" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HT0</accession>
    <name>UBA5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Ubiquitin-like modifier-activating enzyme 5</fullName>
            <shortName>Ubiquitin-activating enzyme 5</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA14526</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">E1-like enzyme which activates UFM1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EAL31677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HT0"/>
    <dbReference type="SMR" id="Q29HT0"/>
    <dbReference type="FunCoup" id="Q29HT0">
        <property type="interactions" value="2463"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HT0"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0287868">
        <property type="protein sequence ID" value="FBpp0286306"/>
        <property type="gene ID" value="FBgn0074554"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0365778">
        <property type="protein sequence ID" value="FBpp0329057"/>
        <property type="gene ID" value="FBgn0074554"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814656"/>
    <dbReference type="eggNOG" id="KOG2336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_0_1_1"/>
    <dbReference type="InParanoid" id="Q29HT0"/>
    <dbReference type="OMA" id="MNIVKDY"/>
    <dbReference type="PhylomeDB" id="Q29HT0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074554">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HT0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071566">
        <property type="term" value="F:UFM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071569">
        <property type="term" value="P:protein ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000066">
        <property type="entry name" value="Putative ubiquitin-like modifier-activating enzyme 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF9">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000391947" description="Ubiquitin-like modifier-activating enzyme 5">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="1">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="397" mass="43528" checksum="410636D88DFB3A8D" modified="2006-04-04" version="1">MTNAIDELQAIIAELKTEVEEQRAVIRQSRDRIEHMSAEVVDSNPYSRLMALQRMNIVKNYERIRDKTVAIVGVGGVGSVTADMLTRCGIGKLILFDYDKVELANMNRLFFTPDQAGLSKVEAAARTLTFINPDVRIETHNYNITTIDNFDNFLSTITGDGTVAGEPVDLVLSCVDNFEARMAINAACNEKCLNWFESGVSENAVSGHIQFLRPGDTACFACAPPLVVAENIDEKTLKREGVCAASLPTTMGITAGFLVQNALKYLLNFGEVSDYLGYNALNDFFPKMTLKPNPQCDDRNCLLRQKEFQARPKPVVVQEEAPTDEPLHASNDWGIELVAEDAPSDAPTDLSQSTDVGQGLRLAYEAPEKSSAEATQAATAPVDDTSLEDLMAQMKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HU2</accession>
    <accession>A0A6I8UFE8</accession>
    <name>Q29HU2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vitellogenin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4814550</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354610.2">
        <property type="nucleotide sequence ID" value="XM_001354574.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HU2"/>
    <dbReference type="SMR" id="Q29HU2"/>
    <dbReference type="FunCoup" id="Q29HU2">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HU2"/>
    <dbReference type="ESTHER" id="drops-q29hu2">
        <property type="family name" value="Insect_lipase"/>
    </dbReference>
    <dbReference type="GeneID" id="4814550"/>
    <dbReference type="KEGG" id="dpo:4814550"/>
    <dbReference type="eggNOG" id="ENOG502SRF1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027171_2_1_1"/>
    <dbReference type="InParanoid" id="Q29HU2"/>
    <dbReference type="OMA" id="RDPMGDV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078700">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000122">
        <property type="entry name" value="Vitellogenin-3-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF149">
        <property type="entry name" value="FI01450P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001354610.2"/>
        </source>
    </evidence>
    <sequence length="419" mass="45585" checksum="5A173132702C93F5" modified="2008-10-14" version="2">MGNPRRIHDQHQGRHGLAKMTGRLLLMGLLVVQLARPIQSIEWTLPDVIDSIGSVSTSIIDPSLLPTPQGLLDGSKQLIAGYPFEYVSSTLNFICSQALSSHKIKSKFTPDINKMNFQLQGACTKTNFALTDAESMWKSPEFNPKKKVVILATGWTTTVNGSDTIDVLSKAYNCRGDVNFVAVDAARFVDTLYTWSAFNTEEIGENIAHGLVHLLDLVPVENIHLIGHSLGAHIVGSAGRHLQRLTGKTIPRITGLDPAKPCFNEGEILSGLLRGDAAFIDVIHSNPGVLGKKDPLGDADFYPGGIHPLPEGCYSVVCAHARAWQYYAESVFPGNEHNFMATRCNSMQRLRDLRCPGNEHPMGYAAPTTIRGNYFLEVSGTSPYGKHANIVRTMSHEHCGVCPNGPDSTTTPTSTPSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IU5</accession>
    <accession>A0A6I8VHU8</accession>
    <name>Q29IU5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Protein transport protein Sec61 gamma-2 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">Sec61gamma</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355499.1">
        <property type="nucleotide sequence ID" value="XM_001355463.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041182.1">
        <property type="nucleotide sequence ID" value="XM_015185696.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IU5"/>
    <dbReference type="SMR" id="Q29IU5"/>
    <dbReference type="STRING" id="46245.Q29IU5"/>
    <dbReference type="GeneID" id="4815583"/>
    <dbReference type="KEGG" id="dpo:4815583"/>
    <dbReference type="eggNOG" id="KOG3498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167752_2_0_1"/>
    <dbReference type="OMA" id="KPDQKEY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072874">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IU5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.820:FF:000001">
        <property type="entry name" value="Transport protein Sec61 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001355499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015041182.1"/>
        </source>
    </evidence>
    <sequence length="68" mass="7610" checksum="7FA937CE64BB469E" modified="2006-04-04" version="1">MDKVVKFAEPGRAFAKDSIRLVKRCTKPDRKEFQKIAIATAIGFCIMGFIGFFVKLIHIPINNIIVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JJ7</accession>
    <accession>A0A6I8UGQ4</accession>
    <name>Q29JJ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Required for meiotic nuclear division protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4815691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355247.2">
        <property type="nucleotide sequence ID" value="XM_001355211.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JJ7"/>
    <dbReference type="SMR" id="Q29JJ7"/>
    <dbReference type="FunCoup" id="Q29JJ7">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JJ7"/>
    <dbReference type="GeneID" id="4815691"/>
    <dbReference type="KEGG" id="dpo:4815691"/>
    <dbReference type="eggNOG" id="KOG2861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011220_4_1_1"/>
    <dbReference type="InParanoid" id="Q29JJ7"/>
    <dbReference type="OMA" id="KLGMWEA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071194">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JJ7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF1">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355247.2"/>
        </source>
    </evidence>
    <sequence length="426" mass="49178" checksum="9FB43A8C31A10E5D" modified="2008-10-14" version="2">MNFARLRLEKLILRSLRQPQRHFQTQWQGHRWMTTITKPPATAAVAEETDAATKKSKSATIQGQAPSVKPMPLVEERLESILSPIRTRIMRKKRLATDELSALGFLNTRGYTTAEEYNLEALQAALKEQNLYETKRFFSTDNLDVEQNVLFVAAKYPTGQQPREIFFFREGSVVFWNCSDIEMNNVLNFLRSFERESYVSALVHGESEVMPYTYIPSTAVDVEGDLVAESSDFNATSRAFFQKGKFFVTGDRDSFLYKYTFSNAMAQSIKLGMWEATLDRYIDSIEHLTEDLKRGRRLRISRAAMLRKTGELFALRHVINLSSDLLDAPDFYWDREELEGLYLQVCSYFSISRRTKVMNEKISHCVELAELVSHNLNDAHHIRLEWMIIILIMVEVGFEILHFISDKGDKQQSLEIVPLSNQLVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LK2</accession>
    <accession>A0A6I8UKJ5</accession>
    <name>Q29LK2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA selenocysteine-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Secp43</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM TRSPAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356977.2">
        <property type="nucleotide sequence ID" value="XM_001356941.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LK2"/>
    <dbReference type="SMR" id="Q29LK2"/>
    <dbReference type="FunCoup" id="Q29LK2">
        <property type="interactions" value="1750"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LK2"/>
    <dbReference type="GeneID" id="4817572"/>
    <dbReference type="KEGG" id="dpo:4817572"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016304_3_0_1"/>
    <dbReference type="InParanoid" id="Q29LK2"/>
    <dbReference type="OMA" id="YDMNGYV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073767">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LK2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12610">
        <property type="entry name" value="RRM1_SECp43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12345">
        <property type="entry name" value="RRM2_SECp43_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000159">
        <property type="entry name" value="tRNA selenocysteine 1-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000166">
        <property type="entry name" value="Trna selenocysteine 1-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040434">
        <property type="entry name" value="TSAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041085">
        <property type="entry name" value="TSAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37457">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37457:SF3">
        <property type="entry name" value="TRNA SELENOCYSTEINE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17654">
        <property type="entry name" value="Trnau1ap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="259"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001356977.2"/>
        </source>
    </evidence>
    <sequence length="339" mass="37649" checksum="6392EC47ADA97CDF" modified="2008-10-14" version="2">MTSVHCQLWMGSLESYMTENFIIAAFRKMGEDPTTVRLMRNKYTGEPAGYCFVNFISDDHALDAMHKLNGKPIPGTNPIVRFRLNSASNSYKLPGNEREFSVWVGDLSSDVDDYQLYKIFSSKFTSIKTAKVILDSLGFSKGYGFVRFGIEDEQKSALYDMNGFIGLGTKPIKICNAVPKPKSELGAALGVSGSTNYGYSGAGGTSSPAAGADYSQYYDPTSTYWQGYNAWQGYYEQAGPSITDAAAYYQQAMSQSHSNPQTLAQHAEAWSAQRSAQYEQQQQQSAASNGETDPDFGLVEHKFVLDVDKMNREAIDSDRRLYDALESSKWLPIEQLEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LW7</accession>
    <accession>A0A6I8UKK1</accession>
    <name>Q29LW7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TM9SF2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356862.2">
        <property type="nucleotide sequence ID" value="XM_001356826.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LW7"/>
    <dbReference type="FunCoup" id="Q29LW7">
        <property type="interactions" value="1524"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LW7"/>
    <dbReference type="GeneID" id="4817108"/>
    <dbReference type="KEGG" id="dpo:4817108"/>
    <dbReference type="eggNOG" id="KOG1278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010714_4_1_1"/>
    <dbReference type="InParanoid" id="Q29LW7"/>
    <dbReference type="OMA" id="KVYYMFG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081681">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LW7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF176">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035543880" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="27"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="432"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="522"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="551"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="591"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="622"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356862.2"/>
        </source>
    </evidence>
    <sequence length="661" mass="74873" checksum="C2E3CE21A7895937" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NJ7</accession>
    <accession>A0A6I8UL23</accession>
    <name>Q29NJ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger protein 595</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Plzf</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357155.2">
        <property type="nucleotide sequence ID" value="XM_001357119.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NJ7"/>
    <dbReference type="SMR" id="Q29NJ7"/>
    <dbReference type="FunCoup" id="Q29NJ7">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="GeneID" id="4817759"/>
    <dbReference type="KEGG" id="dpo:4817759"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_560510_0_0_1"/>
    <dbReference type="InParanoid" id="Q29NJ7"/>
    <dbReference type="OMA" id="CIGCDYK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079861">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="420"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001357155.2"/>
        </source>
    </evidence>
    <sequence length="480" mass="55129" checksum="FED723F66854B6B1" modified="2008-10-14" version="2">MLLAPAVQSLPTLHQPLHSKVSNYLNNQRRTGQFCDLLLELDSDEALSLSVHFCVLAAQSKLIGTNQKQQQFSIHNPLKITIRNFACTQCLHTIVDFFYEDIVSVSKEHEEHFRELAKILAVTELLNLYQMQPVDEVRDTCEATTSGEAEVNLETTKKPEDLFENQQSFFKLKNPRAVKSSSKVNYCIGCDFKCYQVQKMIEHMSSCEPSHLTCSLCEVGFLDWREHDKHLRRHSGDLRKPFFCLECGIHFTTRAALLVHQPKHSTETPHICPHCGKGFKWKQGLSNHILVHNPEKQMLCDVCGYSTTHMKALKSHKLLHTGEFFACTVSGCKHRANRKENLKLHIETHKQGRDFICEICGCKFSQSKNLKRHALKHTENGLNRYKCQLCGFSSHRSDKMKEHVQRVHTEKAVQLELSESVDSSFENKMPDDFDLPMIEDTLPPGKKKQREPKGSRKQTEENTSSSQKRLKTLLPKESFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29P33</accession>
    <accession>A0A6I8ULP6</accession>
    <name>Q29P33_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUZ RNA-binding domain-containing</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4817948</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SZRD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357391.2">
        <property type="nucleotide sequence ID" value="XM_001357355.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29P33"/>
    <dbReference type="FunCoup" id="Q29P33">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29P33"/>
    <dbReference type="GeneID" id="4817948"/>
    <dbReference type="KEGG" id="dpo:4817948"/>
    <dbReference type="eggNOG" id="ENOG502RZH5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_772275_0_0_1"/>
    <dbReference type="InParanoid" id="Q29P33"/>
    <dbReference type="OMA" id="NSYFQGP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246817">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29P33">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024642">
        <property type="entry name" value="SUZ-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039228">
        <property type="entry name" value="SZRD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31796">
        <property type="entry name" value="SUZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31796:SF2">
        <property type="entry name" value="SUZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12901">
        <property type="entry name" value="SUZ-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="27"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="266"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="283"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357391.2"/>
        </source>
    </evidence>
    <sequence length="350" mass="38368" checksum="6C0C80ADD253E7A9" modified="2008-10-14" version="2">MSHGEDVLDNWEELDEAALSMTLQTKLQTSEGKPVMKMKLLQRPQSLQDSSSGSPSSAPRQITIAKKPTPLEAAQSDGEPVLMVLHKSTNDYDPATYATPTINQTVKILRRPAQAEERRDTNGMKPKQPIKTLKQREQEYAEARLRILGAAKNPEDDKPPTPPIPVSPQQASPAAANHSTLPAVSHNNNNNNNNSNPGPGSGMHRSSSAPKMSQVSAMYNNYNSYFQGPHNPGLNYYYQHAGPLPPQQQPPAMPPHFNQRMPPYVGGGMGMGGMPAQPPPQPSVNQQQQQQSWSPVVGGSVSAALLRQQSMQQSPQQQHQHQPPQNYNDNILRLPRGPCPNGSVGFQMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ17</accession>
    <accession>A0A6I8UDV8</accession>
    <name>Q2LZ17_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">MED19</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353956.2">
        <property type="nucleotide sequence ID" value="XM_001353920.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ17"/>
    <dbReference type="FunCoup" id="Q2LZ17">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZ17"/>
    <dbReference type="GeneID" id="4813107"/>
    <dbReference type="KEGG" id="dpo:4813107"/>
    <dbReference type="CTD" id="219541"/>
    <dbReference type="eggNOG" id="KOG4043">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_829689_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LZ17"/>
    <dbReference type="OMA" id="AGMSNPM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078959">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZ17">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019403">
        <property type="entry name" value="Mediator_Med19_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536">
        <property type="entry name" value="LUNG CANCER METASTASIS-RELATED LCMR1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10278">
        <property type="entry name" value="Med19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353956.2"/>
        </source>
    </evidence>
    <sequence length="338" mass="35528" checksum="5E7798616C68B104" modified="2008-10-14" version="2">MMSNYGNIMTDSPRSSPHGGRSPVVARQDSSGTLKTTISLGKTPTIIHTGPFYSMKEPPAKAELTGDKDLMTEYGLHHTLTKFKEKKFKESLSSFLQNIPGINDLITHPVENSTLRSVIEKPPIGGKELLPLTAVQLAGFRLHPGPLPEQYRTTYVTPARKHKNKHKKHKHKDGVTTGQESTLLDSTGLETYEKKHKKQKRHEDDKERKKRKKEKKRKKKNQSPEPGVGLLPGGAGLGNAAGGGIMGATGLGSLGGGPGPGAGTGMSNLSASMGPGVVGMNNFSSSMQMQQQQMPMQQQQMTSGGLLGSVLGVGGGPGGGGGGAGSGGGGGSLLMSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UA93</accession>
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            <fullName evidence="3">Protein anon-37Cs</fullName>
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    </protein>
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        <name evidence="3" type="primary">LOC4811692</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_001352238.4"/>
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    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF398">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="52"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352274.3"/>
        </source>
    </evidence>
    <sequence length="510" mass="56800" checksum="8D73C214B2545F4C" modified="2020-08-12" version="1">MQCYKLSARRSLYNATALQSDLSNQNHGHAIGNLDLESARQNARIVVIGAGLAGLSAAQHLLSHGFRRTVVLEATERYGGRINSQRFGDTFCELGAKWVPIDGSQDSTYELLRNVEGLGKRIKQPDRPEYVQIDHDQNKVNPVMVDLIDALFRQLCGGLKVSDKVKTGSDLHSLDNVMAYFRTESNKAIELSFKPEEQNTAREIFQSLFKEFSSVLGCCLEYVNIDHITSCPVQPDPIYVPTGLDNIVDELTQRLGKEQLQTGKPVGQIQWTPSALGHHNSVGCLDGSLYNADHIICTLPLGVLKNFAGILFRPSLPQEKMMAIRNLGFGSPVKIYLSYNLPIRLWLRRNLRPLGTLINRVTDPQAERSWTQQVVEISQVPSSQHVLEVRVGGGYYEEIEKLPDTRLLEQITTLLRKCISNPLVPYPQEILRSKWSTSACYLGGRPYFSTCSSARDVQRLAAPLGGKAPSLLFAGDATALHGFGTIDAARSSGIREAQRIIEYYNIKQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAP9</accession>
    <name>A0A6I8UAP9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Transient receptor potential channel pyrexia</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">pyx</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352500.3">
        <property type="nucleotide sequence ID" value="XM_001352464.4"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A6I8UAP9"/>
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        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812573"/>
    <dbReference type="InParanoid" id="A0A6I8UAP9"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAP9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052076">
        <property type="entry name" value="TRP_cation_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47143:SF1">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47143">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL PROTEIN PAINLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="569"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="598"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="642"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="704"/>
            <end position="726"/>
        </location>
    </feature>
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        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="168"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
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        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
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        <location>
            <begin position="267"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="300"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="368"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="502"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="835"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="890"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="837"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="851"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001352500.3"/>
        </source>
    </evidence>
    <sequence length="958" mass="108716" checksum="6DCC3EF2956A6AAF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAX2</accession>
    <name>A0A6I8UAX2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retinin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352562.3">
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    <dbReference type="AlphaFoldDB" id="A0A6I8UAX2"/>
    <dbReference type="KEGG" id="dpo:4812144"/>
    <dbReference type="InParanoid" id="A0A6I8UAX2"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007614">
        <property type="entry name" value="Retinin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931">
        <property type="entry name" value="FI02976P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931:SF4">
        <property type="entry name" value="GEO13385P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04527">
        <property type="entry name" value="Retinin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026126993" description="Retinin" evidence="1">
        <location>
            <begin position="23"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352562.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11149" checksum="55B8C1CD0BFB4991" modified="2020-08-12" version="1">MPKQLCLFVAVVLLCLVAGGQTELHIYHPLLTLHQEPTLARVGNLLEHVPTAVSHQSSTIVHRTAPRLTPLLAPALRTTLHYPPTWSYPFYGATNSLYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBK9</accession>
    <name>A0A6I8UBK9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352915.4">
        <property type="nucleotide sequence ID" value="XM_001352879.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBK9"/>
    <dbReference type="FunCoup" id="A0A6I8UBK9">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812299"/>
    <dbReference type="InParanoid" id="A0A6I8UBK9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBK9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031720">
        <property type="entry name" value="DUF4728"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36694:SF10">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36694">
        <property type="entry name" value="PASIFLORA 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15860">
        <property type="entry name" value="DUF4728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352915.4"/>
        </source>
    </evidence>
    <sequence length="376" mass="42533" checksum="3BDC8331CF4DD431" modified="2020-08-12" version="1">MKSVILYPYGPLTGGTRCCRMLHAKNLAITSAIYTINISLLIVLIYSWRINVNMHKSSDLQDVYYGVQIAYFAIIGTQISMILLSIVLIFGICRENPGLIVPWIIGYITFMALEAVAMVYSNVLRDHVNKQFDAMCKAEVAFFIARALINVLAMWGVLRFYNLVRSGITWRGPEAKTAIFAPMYKSVSTRSGKSHHSKRHSKRRHSMESGAGCCAIFDFDFDYDDDDDDDDDDDEAAYGDGMETEDDVGYDYDDYYDYYEDEEEQQQQQLELEPQDDADCSMLGGPRKTRSRSRSRPRSRPRSAVVKVKGQRSGGRRTNNNIRSVLINKRHNKYKIARPQTQLEVINESERSAGSGSDENDSLLVAYDSSSSLASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UD11</accession>
    <name>A0A6I8UD11_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein sneaky</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">snky</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353592.3">
        <property type="nucleotide sequence ID" value="XM_001353556.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UD11"/>
    <dbReference type="FunCoup" id="A0A6I8UD11">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812762"/>
    <dbReference type="InParanoid" id="A0A6I8UD11"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051856">
        <property type="entry name" value="CSR-E3_Ligase_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012858">
        <property type="entry name" value="DC_STAMP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041">
        <property type="entry name" value="DENDRITIC CELL-SPECIFIC TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041:SF17">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DCST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07782">
        <property type="entry name" value="DC_STAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="545"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Dendritic cell-specific transmembrane protein-like" evidence="7">
        <location>
            <begin position="399"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353592.3"/>
        </source>
    </evidence>
    <sequence length="713" mass="82876" checksum="EEA96134C77CE693" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDR5</accession>
    <name>A0A6I8UDR5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Contactin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353932.3">
        <property type="nucleotide sequence ID" value="XM_001353896.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDR5"/>
    <dbReference type="KEGG" id="dpo:4813042"/>
    <dbReference type="InParanoid" id="A0A6I8UDR5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDR5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="123"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353932.3"/>
        </source>
    </evidence>
    <sequence length="208" mass="23893" checksum="DE24D91028F40F6D" modified="2020-08-12" version="1">MGVEWITCRADAERSIKTTFPVDDRRSESRGSTMTSLCGLWSWKLKQSSLLGLGLCLLLLISDLGRVTAQRSTGGRNVLRRNYTGKKPVVIQHLSQDAVSYYDHENGAKIIKSSHFELDYTLGRKITFFCMAQGNPRPTITWFKDGAELYQHRFFQVHESHIETDIIKSKMEIDPTTQMDAGYYECQADNIYAIDRRGFRTDYVMVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE90</accession>
    <name>A0A6I8UE90_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Ecdysone-induced protein 78C isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">Eip78C</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15 16">MV-25-SWS-2005</strain>
            <tissue evidence="15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354071.4">
        <property type="nucleotide sequence ID" value="XM_001354035.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239985.1">
        <property type="nucleotide sequence ID" value="XM_033384094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE90"/>
    <dbReference type="KEGG" id="dpo:4813880"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07165">
        <property type="entry name" value="NR_DBD_DmE78_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06941">
        <property type="entry name" value="NR_LBD_DmE78_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000044">
        <property type="entry name" value="Ecdysone-induced protein 78C, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000056">
        <property type="entry name" value="Peroxisome proliferator-activated receptor gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048008">
        <property type="entry name" value="NR_LBD_DmE78-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001728">
        <property type="entry name" value="ThyrH_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
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        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 78C"/>
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    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
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            <begin position="455"/>
            <end position="530"/>
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            <begin position="529"/>
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        <location>
            <begin position="580"/>
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    </feature>
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        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
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        <location>
            <begin position="53"/>
            <end position="89"/>
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    </feature>
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        <location>
            <begin position="199"/>
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        <location>
            <begin position="330"/>
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        <location>
            <begin position="380"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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            <begin position="611"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51030"/>
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        <source>
            <dbReference type="PROSITE" id="PS51843"/>
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    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001354071.4"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033239985.1"/>
        </source>
    </evidence>
    <sequence length="949" mass="102497" checksum="CCD87863CFC0A846" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGJ4</accession>
    <name>A0A6I8UGJ4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM3</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Mcm3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
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        <property type="nucleotide sequence ID" value="XM_001355095.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGJ4"/>
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        <property type="interactions" value="1268"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815244"/>
    <dbReference type="InParanoid" id="A0A6I8UGJ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MCM3"/>
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        <property type="entry name" value="DNA helicase"/>
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        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
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    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="MCM"/>
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        <property type="entry name" value="MCM_CS"/>
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    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056575">
        <property type="entry name" value="WH_MCM3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF46">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23191">
        <property type="entry name" value="WHD_MCM3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01659">
        <property type="entry name" value="MCMPROTEIN3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="290"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="658"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="730"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="744"/>
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        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001355131.2"/>
        </source>
    </evidence>
    <sequence length="826" mass="91917" checksum="D3BD4AE98E938496" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGW2</accession>
    <name>A0A6I8UGW2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Thioredoxin domain-containing protein 5 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">prtp</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355336.2">
        <property type="nucleotide sequence ID" value="XM_001355300.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGW2"/>
    <dbReference type="FunCoup" id="A0A6I8UGW2">
        <property type="interactions" value="1130"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815689"/>
    <dbReference type="InParanoid" id="A0A6I8UGW2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGW2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03005">
        <property type="entry name" value="PDI_a_ERp46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000398">
        <property type="entry name" value="Thioredoxin domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026144426" evidence="7">
        <location>
            <begin position="27"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="20"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="146"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="298"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355336.2"/>
        </source>
    </evidence>
    <sequence length="421" mass="47209" checksum="2759AE39C80FC3D0" modified="2020-08-12" version="1">MLMRSLVPITVCAFFVSPFLSATVRAEEEAVKNAEKQFAVELDPEKFNQAVQSGNVFVKFFAPWCGHCKRLHPLWEQLAEIMNVEDPKVIIAKVDCTKHQALCAEHQVTGYPTLRLFKLGDTESVKFKGTRDLPAITDFINHELNTLSEVEQAEATLEENGGNTVPVANQNLGKVVDLSEDTFAKHVSSGNHFVKFFAPWCSHCQRLAPTWDELAKEIKHISGVTVSKIDCTQYRSVCQDFEVKGYPTLLWIEDGKKIEKYSGARDLPTLKSYVEKMVGVPMDKKEVSDTSAQDAAKEKADEEAGKLKPQQLNGEVAFTQAVAEGIAFIKFYAPWCGHCQKLQPTWEQLATETHNSQSGVVIAKVDCTAPENKQICIDEQVEGYPTLFLYRNGQRQDEYEGSRTLPELKAYLKKSIGHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHD6</accession>
    <name>A0A6I8UHD6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glucose dehydrogenase [FAD, quinone]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4815547</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355516.3">
        <property type="nucleotide sequence ID" value="XM_001355480.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHD6"/>
    <dbReference type="FunCoup" id="A0A6I8UHD6">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815547"/>
    <dbReference type="InParanoid" id="A0A6I8UHD6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHD6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF158">
        <property type="entry name" value="GH23626P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026089774" evidence="4">
        <location>
            <begin position="31"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="564"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="608"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001355516.3"/>
        </source>
    </evidence>
    <sequence length="632" mass="70653" checksum="B29DF42FC650615D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHN5</accession>
    <name>A0A6I8UHN5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 19">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="13">IMP dehydrogenase</shortName>
            <shortName evidence="13">IMPD</shortName>
            <shortName evidence="13">IMPDH</shortName>
            <ecNumber evidence="13 19">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22 23" type="primary">ras</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22 23">MV-25-SWS-2005</strain>
            <tissue evidence="22 23">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13 19">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13 19">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13 18">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="13 19"/>
    <dbReference type="RefSeq" id="XP_001355544.3">
        <property type="nucleotide sequence ID" value="XM_001355508.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041220.2">
        <property type="nucleotide sequence ID" value="XM_015185734.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHN5"/>
    <dbReference type="KEGG" id="dpo:4815444"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
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        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chromosome 19 SCAF14664, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
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    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
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    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
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    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
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        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
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        <property type="entry name" value="IMP_DH_GMPRt"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="5 13 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13 15" id="KW-0520">NAD</keyword>
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    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="20">
        <location>
            <begin position="125"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="20">
        <location>
            <begin position="189"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="13 14">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13 14">
        <location>
            <position position="440"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="282"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="332"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 16">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 16">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 16">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="372"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="395"/>
            <end position="396"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="419"/>
            <end position="423"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
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    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03156"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001355544.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_015041220.2"/>
        </source>
    </evidence>
    <sequence length="526" mass="56815" checksum="8C824A087DFEE9C6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJD3</accession>
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        <submittedName>
            <fullName evidence="10">Neurogenic protein big brain</fullName>
        </submittedName>
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    <gene>
        <name evidence="10" type="primary">bib</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
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        <property type="nucleotide sequence ID" value="XM_001356284.4"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glycerol uptake facilitator protein"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
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    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
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        <property type="entry name" value="MIP_CS"/>
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        <property type="entry name" value="MIP"/>
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    </dbReference>
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        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
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    <dbReference type="PANTHER" id="PTHR19139:SF268">
        <property type="entry name" value="NEUROGENIC PROTEIN BIG BRAIN"/>
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    </dbReference>
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        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MINTRINSICP"/>
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            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="310"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="431"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="454"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="479"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <begin position="544"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <begin position="554"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <begin position="579"/>
            <end position="590"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
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            <begin position="602"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="643"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="662"/>
            <end position="683"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356320.4"/>
        </source>
    </evidence>
    <sequence length="693" mass="76328" checksum="B10C6CABED7BDF0C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJT9</accession>
    <name>A0A6I8UJT9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Early growth response protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4817069</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356661.4">
        <property type="nucleotide sequence ID" value="XM_001356625.4"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8UJT9"/>
    <dbReference type="FunCoup" id="A0A6I8UJT9">
        <property type="interactions" value="128"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000112">
        <property type="entry name" value="Mds1 and evi1 complex locus protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000159">
        <property type="entry name" value="Mds1 and evi1 complex locus protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000929">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051643">
        <property type="entry name" value="Transcr_Reg_ZincFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396:SF21">
        <property type="entry name" value="ZINC FINGER PROTEIN 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="340"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="245"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="434"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="501"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="436"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="509"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356661.4"/>
        </source>
    </evidence>
    <sequence length="564" mass="61504" checksum="3C12EB37C0ECBA53" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKV8</accession>
    <name>A0A6I8UKV8_DROPS</name>
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        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="10 11" type="primary">LOC4817780</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable E2 ubiquitin-protein ligase that catalyzes the covalent attachment of ubiquitin to target proteins. May facilitate the monoubiquitination and degradation of MTOR and CCNE1 through interaction with FBXW7.</text>
    </comment>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBC-like"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="121"/>
            <end position="282"/>
        </location>
    </feature>
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            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
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            <begin position="91"/>
            <end position="112"/>
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        <location>
            <begin position="21"/>
            <end position="59"/>
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    </feature>
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            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055455"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50127"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001357144.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015035673.2"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL88</accession>
    <name>A0A6I8UL88_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4817845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357301.3">
        <property type="nucleotide sequence ID" value="XM_001357265.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL88"/>
    <dbReference type="KEGG" id="dpo:4817845"/>
    <dbReference type="InParanoid" id="A0A6I8UL88"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026042578" evidence="2">
        <location>
            <begin position="24"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357301.3"/>
        </source>
    </evidence>
    <sequence length="178" mass="20443" checksum="787F7EFFA1E527CF" modified="2020-08-12" version="1">MCKFTNRLFVFALLLLALGVVLAQPVSVKARPGGPRNVISDLLEVLYDDYNDSGYQQEDIYYDQRQKGAENLQLKMDGLVVALAPEMSYHSLYMMADQYFNMREMLLQHSTPEPDETQLYEKVPVREPESTASEEHTRIPDPKTDMESRQHHVVAPQNASRSPSQLLEMLKKLKSRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULQ5</accession>
    <name>A0A6I8ULQ5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">bin3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
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</entry>
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            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001357648.2"/>
        </source>
    </evidence>
    <sequence length="1722" mass="189049" checksum="3AD178A2DF578A36" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMI3</accession>
    <name>A0A6I8UMI3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8 9">Peroxisomal carnitine O-octanoyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8 9" type="primary">CROT</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <strain evidence="6">MV2-25</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357904.2">
        <property type="nucleotide sequence ID" value="XM_001357867.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038506.2">
        <property type="nucleotide sequence ID" value="XM_015183020.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038507.2">
        <property type="nucleotide sequence ID" value="XM_015183021.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMI3"/>
    <dbReference type="KEGG" id="dpo:4800672"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008458">
        <property type="term" value="F:carnitine O-octanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF67">
        <property type="entry name" value="PEROXISOMAL CARNITINE O-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="5">
        <location>
            <begin position="26"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001357904.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015038506.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015038507.2"/>
        </source>
    </evidence>
    <sequence length="618" mass="70405" checksum="8176D0C433AE5E30" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNG1</accession>
    <name>A0A6I8UNG1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dynein axonemal assembly factor 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Defective transmitter-recycling protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Leucine-rich repeat-containing protein 50 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">sds22</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium-specific protein required for cilia structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SDS22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358334.3">
        <property type="nucleotide sequence ID" value="XM_001358297.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNG1"/>
    <dbReference type="FunCoup" id="A0A6I8UNG1">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801207"/>
    <dbReference type="InParanoid" id="A0A6I8UNG1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000055">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000446">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit SDS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF23">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358334.3"/>
        </source>
    </evidence>
    <sequence length="327" mass="37728" checksum="2E154E50C2C30938" modified="2020-08-12" version="1">MGDADRAMNTNSEPVKPPVGLQVIAPEDMASIEETITIDPECFELDLNHRRIEKLENFEPLTQIQRLFLRWNLIKKIENLSMLKTLVELELYDNQITKVENLEELTKLEMLDLSFNRLTKIENLDALVNLEKLYFVANRITVIENLGMLTSLTMLELGDNKLKKIQNIDTLVNLRQLFLGKNKIAKIENLDTLVNLEILSLQANRIVKIENLEKLTSLKELYISENGIEVIENLEENKNLETLDLAKNRLKAVGNLETLENLEELWLNHNGIDDWKNIELLKGNKALQTIYLESNPLAKDVRYRSKLRDILPQLQKIDATMCVMPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNK2</accession>
    <name>A0A6I8UNK2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">N-acetyltransferase 9-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Mnat9</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNAT subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358261.3">
        <property type="nucleotide sequence ID" value="XM_001358224.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNK2"/>
    <dbReference type="FunCoup" id="A0A6I8UNK2">
        <property type="interactions" value="1113"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801114"/>
    <dbReference type="InParanoid" id="A0A6I8UNK2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000132">
        <property type="entry name" value="N-acetyltransferase 9-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039135">
        <property type="entry name" value="NAT9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256:SF16">
        <property type="entry name" value="ALPHA_BETA-TUBULIN-N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256">
        <property type="entry name" value="N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="34"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358261.3"/>
        </source>
    </evidence>
    <sequence length="199" mass="23189" checksum="112087C61C44FBA5" modified="2020-08-12" version="1">MRLNEKTKLLGKKVVLVPYEARHVPKYHDWMSNETLRDLTASEELTLSEEYLMQLSWRQDTDKLTFIVLAADIYARDNDEIAAMVGDTNLFLRHEPDTNQKVAEAEIMIAEPQARGKGYGREAMLLMMKYAQSQLELNKFEVKIDMDNAVSLRLFESFQFVETGRVEVFHEVTMEREITTEWIGWLDKQVDLTIESYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPY9</accession>
    <name>A0A6I8UPY9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glucose 1-dehydrogenase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4802044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359037.2">
        <property type="nucleotide sequence ID" value="XM_001359000.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPY9"/>
    <dbReference type="FunCoup" id="A0A6I8UPY9">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802044"/>
    <dbReference type="InParanoid" id="A0A6I8UPY9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43975:SF2">
        <property type="entry name" value="EG:BACR7A4.14 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43975">
        <property type="entry name" value="ZGC:101858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359037.2"/>
        </source>
    </evidence>
    <sequence length="256" mass="27063" checksum="7CD2B2D20AA0EE58" modified="2020-08-12" version="1">MNFAGKVILITGASSGIGAATALKFARLGACLALNGRNVDNLKKVAQQCSEASKSAPALVVGDISKAEDIERVWADTLRAYGKLDVLVNNAGILETGTIENTSLEQYDRLMNTNVRAIYHLTMLATPELIKTKGNIVNVSSVNGIRSFPGVLAYNVSKAAVDQFTRCVALELAPKGVRVNSVNPGVIITQLQRRGGLDDEAYAKFLEHAKVTHALGRPGDVSEVAAAIAFLASNEASFTTGISLPVDGGRHAMCPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQB7</accession>
    <name>A0A6I8UQB7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4802139</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359118.2">
        <property type="nucleotide sequence ID" value="XM_001359081.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQB7"/>
    <dbReference type="FunCoup" id="A0A6I8UQB7">
        <property type="interactions" value="1431"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802139"/>
    <dbReference type="InParanoid" id="A0A6I8UQB7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040024">
        <property type="entry name" value="PPP1R21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049372">
        <property type="entry name" value="PPP1R21_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019343">
        <property type="entry name" value="PPP1R21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21448:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21448">
        <property type="entry name" value="SMOOTH MUSCLE MYOSIN HEAVY CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10205">
        <property type="entry name" value="KLRAQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21636">
        <property type="entry name" value="PPP1R21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01254">
        <property type="entry name" value="KLRAQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 21 N-terminal" evidence="3">
        <location>
            <begin position="18"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="346"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359118.2"/>
        </source>
    </evidence>
    <sequence length="417" mass="46965" checksum="32FAA36D4F8A37C7" modified="2020-08-12" version="1">MAEKAMTMDGTVSADKYQKLASEYSKLRAQATVLKKAVLEEQAKEGGLREQLQQYATSLRRTEQEVDSLGFRNKQLESRVSQLQQEISVHEQQRKKKDKEPASRRGLLGGTKLDVGDAASAETSHEANAAQETLLFEELQKKIMENAQLTSLIDDKQRDLLLHTERIATLSQKLEKRIGDQNELEKRLRKEIETLQHRNSELETKLVDAASMLGSEDALSATGSDNTPLHNLQQNNSNHVVQLTSEDRIAMLEKEAAHWRAQYEVAKLQQNFNSNPIKDLSICSCSTAAAGITVKPSELEARGSQRARDSMQEPPEPPSKEQLIYSVFSKKFEDLLRLKVHAESRLRSFELEVQHLQNCLENATHELKAKDEQLASLGGALQMMEEELTTTRINYEEQISVLTEQVISLSDQLAACK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQS4</accession>
    <name>A0A6I8UQS4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Glycogen [starch] synthase</fullName>
            <ecNumber evidence="3 10">2.4.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Glys</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18549"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:18550"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.11" evidence="3 10"/>
    <dbReference type="RefSeq" id="XP_001359328.4">
        <property type="nucleotide sequence ID" value="XM_001359291.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQS4"/>
    <dbReference type="FunCoup" id="A0A6I8UQS4">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802404"/>
    <dbReference type="InParanoid" id="A0A6I8UQS4"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03793">
        <property type="entry name" value="GT3_GSY2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000014">
        <property type="entry name" value="Glycogen [starch] synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000028">
        <property type="entry name" value="Glycogen [starch] synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008631">
        <property type="entry name" value="Glycogen_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176:SF3">
        <property type="entry name" value="GLYCOGEN [STARCH] SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176">
        <property type="entry name" value="GLYCOGEN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05693">
        <property type="entry name" value="Glycogen_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="667"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359328.4"/>
        </source>
    </evidence>
    <sequence length="710" mass="81955" checksum="A1F35DD0D97F6890" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQT0</accession>
    <name>A0A6I8UQT0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc transporter ZIP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Zip89B</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359463.3">
        <property type="nucleotide sequence ID" value="XM_001359426.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQT0"/>
    <dbReference type="FunCoup" id="A0A6I8UQT0">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802568"/>
    <dbReference type="InParanoid" id="A0A6I8UQT0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF203">
        <property type="entry name" value="FI18611P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="417"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="443"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359463.3"/>
        </source>
    </evidence>
    <sequence length="498" mass="54440" checksum="D802812F4C1FC0D9" modified="2020-08-12" version="1">MNQTQVNNFLKCFLQIDGSNSHWTARHDEDGEADHDHEHDEEHDDDTSGVLIAKVTAMVVLCFASTICGSIPFLLNRCYRWTENQTNARSAIVVKCLLYFGGGVLLATTFLHLLPEVQEVVEELQECGVIGELTFPLAELLMCCGFFLMYFIEEAMHTYVDRHQQDPAGAAFERGHSIRNSHLLKANEPSAPPANGASNATSEMGTLSVQNLLQNDLEQQKFGAKQQQALPHDSHMHHHGHSHGHGHGHSHLPVVMDDASSADMLTSSLRGLFIVSALSLHELFEGMAIGLESSASSVWFMFGAVSAHKLVLAFCVGVELIVARTRFTLAILYVLTFAVVSPLGIGIGILISNGQETGPNLASAILQGFACGTLIYVVFFEILSKNRSGLRAYLALFVGFLVMFGLQQISASGGHGHSHSHSSCSASTDDHQETETRSGSGGHSHDHEHDHDHAHAHLPPHHNHAEELAAVHNYEQQLQVLRQVTQKLLEGAHPKKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URH6</accession>
    <name>A0A6I8URH6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein wa-cup</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">wa-cup</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359766.2">
        <property type="nucleotide sequence ID" value="XM_001359729.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URH6"/>
    <dbReference type="FunCoup" id="A0A6I8URH6">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="GeneID" id="4802953"/>
    <dbReference type="KEGG" id="dpo:4802953"/>
    <dbReference type="InParanoid" id="A0A6I8URH6"/>
    <dbReference type="OMA" id="RMRFAEY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070141">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21391">
        <property type="entry name" value="AT04489P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21391:SF0">
        <property type="entry name" value="AT04489P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001359766.2"/>
        </source>
    </evidence>
    <sequence length="565" mass="65634" checksum="AF5FDEBD0B1E28AB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URN0</accession>
    <name>A0A6I8URN0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Parafibromin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hyx</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC73 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359831.1">
        <property type="nucleotide sequence ID" value="XM_001359794.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URN0"/>
    <dbReference type="SMR" id="A0A6I8URN0"/>
    <dbReference type="FunCoup" id="A0A6I8URN0">
        <property type="interactions" value="2518"/>
    </dbReference>
    <dbReference type="GeneID" id="4803029"/>
    <dbReference type="KEGG" id="dpo:4803029"/>
    <dbReference type="InParanoid" id="A0A6I8URN0"/>
    <dbReference type="OMA" id="CAFHLKY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071370">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11990:FF:000002">
        <property type="entry name" value="protein CDC73 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11990">
        <property type="entry name" value="RNA polymerase II accessory factor, Cdc73 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007852">
        <property type="entry name" value="Cdc73/Parafibromin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031336">
        <property type="entry name" value="CDC73_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038103">
        <property type="entry name" value="CDC73_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032041">
        <property type="entry name" value="Cdc73_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466">
        <property type="entry name" value="CDC73 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466:SF8">
        <property type="entry name" value="PARAFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05179">
        <property type="entry name" value="CDC73_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16050">
        <property type="entry name" value="CDC73_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paf1 complex subunit Cdc73 N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Cell division control protein 73 C-terminal" evidence="7">
        <location>
            <begin position="378"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="261"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359831.1"/>
        </source>
    </evidence>
    <sequence length="540" mass="61478" checksum="00EA3742811B3DD1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URY4</accession>
    <name>A0A6I8URY4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mgr</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359983.1">
        <property type="nucleotide sequence ID" value="XM_001359946.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URY4"/>
    <dbReference type="SMR" id="A0A6I8URY4"/>
    <dbReference type="FunCoup" id="A0A6I8URY4">
        <property type="interactions" value="1514"/>
    </dbReference>
    <dbReference type="GeneID" id="4803201"/>
    <dbReference type="KEGG" id="dpo:4803201"/>
    <dbReference type="InParanoid" id="A0A6I8URY4"/>
    <dbReference type="OMA" id="YNWDVAQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079805">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000001">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016396">
        <property type="entry name" value="Prefoldin_subunit_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR016396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359983.1"/>
        </source>
    </evidence>
    <sequence length="195" mass="22445" checksum="B2EA040ABF4C8BCB" modified="2020-08-12" version="1">MTGIMDSVEMPKLPENQKTFAGIPEAVFLEEIDTFMTQPENENCEKVLQRLDEQHGKYRFMAYNLEARRRKLKSQIPDLERSLEMVNVLRKEDEERETQFLLSDQVFIKTLVPPTKTVYLWLGASVMLEYPLDEAEALLKQNVTSAVGNLKSVEHDQDFLRDQITTTEVNMARVYNWGVKKRQAAAKTNASSTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTV1</accession>
    <name>A0A6I8UTV1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein fest isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fest</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360840.1">
        <property type="nucleotide sequence ID" value="XM_001360803.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTV1"/>
    <dbReference type="FunCoup" id="A0A6I8UTV1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="4804253"/>
    <dbReference type="KEGG" id="dpo:4804253"/>
    <dbReference type="InParanoid" id="A0A6I8UTV1"/>
    <dbReference type="OMA" id="RQHNKCL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082139">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UTV1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39075">
        <property type="entry name" value="FI19908P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39075:SF1">
        <property type="entry name" value="FI19908P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360840.1"/>
        </source>
    </evidence>
    <sequence length="499" mass="53923" checksum="455A42E205295D4D" modified="2020-08-12" version="1">MVTDPKSVDPKVSTKEVQCQSSDGETSQQEPGSQLSSPKGSRVGIFNAEDFLSRAQMNDKINNILRSPTKPSNGVRQRSVYTNNPSPQSSLRVGMTGGQLSQRTSNISLSSGTDSVGHMDRGSSSSLASSATVGTNTITSGIPKECANYPVGSQSARPYVQLPGIHISSPPQYGPGNMQEIDAGKMAHNGKALTGRYNATPTYGFDNDQNYCLNSQQLPSPMPPPPYALARVSSTRNFELENHTPPSCPGSGPIAMSNGTIQLRLRDGVRIDMTLDKAVRVLNQRSMVAVALSRNCSNSAMIHPNGRILQSGAKVEIVTYDGMKGNNFVRYAKMWYKGVSFTSEACALIYLVDTAGTRTTTDTFTDLTKDYTLAVFYDDSRHGPSYMAEAHEVIANSAYTCTEDGTEIYDINGFRITQAADGLVKVTRVHNKCLIRTSPGNGSATLTTPGIHCTASLGKTSHLFVRRNEKRMHFDGSCFIVRNAGHSAGFNENNLLIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU53</accession>
    <name>A0A6I8UU53_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 57</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tous</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360811.2">
        <property type="nucleotide sequence ID" value="XM_001360774.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU53"/>
    <dbReference type="FunCoup" id="A0A6I8UU53">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="GeneID" id="4804216"/>
    <dbReference type="KEGG" id="dpo:4804216"/>
    <dbReference type="InParanoid" id="A0A6I8UU53"/>
    <dbReference type="OMA" id="FPHCNAV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078121">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000014">
        <property type="entry name" value="AGAP008095-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000271">
        <property type="entry name" value="cilia- and flagella-associated protein 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="376"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="499"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="691"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="814"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="893"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1095"/>
            <end position="1159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360811.2"/>
        </source>
    </evidence>
    <sequence length="1169" mass="135035" checksum="AD6104DE1AAA7B6A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUQ5</accession>
    <name>A0A6I8UUQ5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exosome complex component RRP4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Exosome component 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Rrp4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Component of the RNA exosome core complex (Exo-9), composed of EXOSC1, EXOSC2, EXOSC3, EXOSC4, EXOSC5, EXOSC6, EXOSC7, EXOSC8 and EXOSC9; within the complex interacts with EXOSC4 and EXOSC7. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6. Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. Exo-9 is formed by a hexameric base ring consisting of the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and a cap ring consisting of EXOSC1, EXOSC2 and EXOSC3. The RNA exosome complex associates with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with GTPBP1. Interacts with ZFP36L1 (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361091.1">
        <property type="nucleotide sequence ID" value="XM_001361054.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUQ5"/>
    <dbReference type="FunCoup" id="A0A6I8UUQ5">
        <property type="interactions" value="2084"/>
    </dbReference>
    <dbReference type="GeneID" id="4804548"/>
    <dbReference type="KEGG" id="dpo:4804548"/>
    <dbReference type="InParanoid" id="A0A6I8UUQ5"/>
    <dbReference type="OMA" id="GVNGFIW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077792">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22525">
        <property type="entry name" value="KH-I_Rrp4_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000022">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000038">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21266">
        <property type="entry name" value="S1_RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="14">
        <location>
            <begin position="30"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="RRP4 S1" evidence="16">
        <location>
            <begin position="78"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="15">
        <location>
            <begin position="172"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001361091.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="33540" checksum="9C083663BC2FFB1F" modified="2020-08-12" version="1">MATNATIELALDRVDWCALAERQDVLPRVYTPGEVVMPEAGFMRGHGTFVEDENIKASVAGVIEKVNKLISVRPLKSRYVGEIGDVVVARVIEVQQKRWRVDTNSRLDSILLLSSVNLPGGELRRRSAEDEQMMRRYLDEGDLISAEVQNIFEEGTLSLYTRSLKYGKLSQGVLVKVFPALVKRRKMHFHNLPCGASVILGNNGYIWISPPKSQDQEGGEGGFAQNLGEAVPRNEREVIARLRNCILALAKCKLMLYDTSIQYAYDESLKYEAHELLQQNVIYDIGEQTQARLRDGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW48</accession>
    <name>A0A6I8UW48_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Kalirin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4805390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361799.4">
        <property type="nucleotide sequence ID" value="XM_001361762.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW48"/>
    <dbReference type="FunCoup" id="A0A6I8UW48">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805390"/>
    <dbReference type="InParanoid" id="A0A6I8UW48"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF209">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="159"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001361799.4"/>
        </source>
    </evidence>
    <sequence length="508" mass="58041" checksum="7B249430C68892BD" modified="2020-08-12" version="1">MELFNWFDVISVALTFLAIVIVQVLNVYLHILDDNTSGKTIDVVDTALAKECRSEEAPSSPVSSILTTPPKPNKNGKLEKQTSLLDSDSGISLSSIYTSATTATTAAANSSPTYRTHNGFLSHTLSFETLGDEYHEDEDTLSLENLFPCDQTEIYASRKISFIIDELIQTEVNYVNNLKKGLANYGNLATVEDLPEGLRGEQRQQLLLGNVSEILELHEREILPLMLRNQRDLKGLFDEFAGHFEKNNFYCYVSFTMGKKSSMQLRQDHREWLQAYQTTIKDKLGIDSFLVQPIQRLTRYPLLLQQFISEFYKSGISCKPVLTAVCKLETRMRRALDVVNQAEEIPNIDELNELDLLQLGNFRRATEFDAQHFPTRKKYRAKIFLFDRCLVCTEVRKKRLAYRQHYHWEHVELQRPLELTASNANKIINLLVKQQDKDKPGSGASGSGKREEYSFVAAEASVVKQWLHATHKIIEIARNEHAQRNTFSLPMDLVLGVGLAIWLIWQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXP0</accession>
    <name>A0A6I8UXP0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6903079</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002132797.2">
        <property type="nucleotide sequence ID" value="XM_002132761.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXP0"/>
    <dbReference type="KEGG" id="dpo:6903079"/>
    <dbReference type="InParanoid" id="A0A6I8UXP0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032222">
        <property type="term" value="P:regulation of synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134352.3"/>
        </source>
    </evidence>
    <sequence length="103" mass="11899" checksum="8E001063267EB146" modified="2020-08-12" version="1">MDQKKEDQMQTNSQKSGGHGNKKCTPIRPHQLPIRQTALLTCYMLNADVALLEKGLENTRRQLQLLQMENFERRLEQFCQCLDRQRARVAALSADLDTDLNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1V0</accession>
    <name>A0A6I8V1V0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">LOC6896695</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_002136909.2">
        <property type="nucleotide sequence ID" value="XM_002136873.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038826.2">
        <property type="nucleotide sequence ID" value="XM_015183340.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1V0"/>
    <dbReference type="KEGG" id="dpo:6896695"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634481" description="procollagen-proline 4-dioxygenase" evidence="13">
        <location>
            <begin position="20"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="404"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002136909.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015038826.2"/>
        </source>
    </evidence>
    <sequence length="527" mass="60785" checksum="F5599E2ACA93C094" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2R5</accession>
    <name>A0A6I8V2R5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Larval serum protein 1 beta chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Lsp1beta</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Larval storage protein (LSP) which may serve as a store of amino acids for synthesis of adult proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterohexamer, composed of three subunits, alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the hemocyanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137424.2">
        <property type="nucleotide sequence ID" value="XM_002137388.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2R5"/>
    <dbReference type="FunCoup" id="A0A6I8V2R5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897260"/>
    <dbReference type="InParanoid" id="A0A6I8V2R5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005616">
        <property type="term" value="C:larval serum protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1280.10:FF:000006">
        <property type="entry name" value="Larval serum protein 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1370.10:FF:000003">
        <property type="entry name" value="Larval serum protein 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1520:FF:000002">
        <property type="entry name" value="Larval serum protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1520">
        <property type="entry name" value="Hemocyanin, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.10">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013788">
        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000896">
        <property type="entry name" value="Hemocyanin/hexamerin_mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005203">
        <property type="entry name" value="Hemocyanin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037020">
        <property type="entry name" value="Hemocyanin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005204">
        <property type="entry name" value="Hemocyanin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036697">
        <property type="entry name" value="Hemocyanin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511:SF5">
        <property type="entry name" value="FAT-BODY PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511">
        <property type="entry name" value="LARVAL STORAGE PROTEIN/PHENOLOXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03723">
        <property type="entry name" value="Hemocyanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00372">
        <property type="entry name" value="Hemocyanin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03722">
        <property type="entry name" value="Hemocyanin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00187">
        <property type="entry name" value="HAEMOCYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48050">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00210">
        <property type="entry name" value="HEMOCYANIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0758">Storage protein</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026216565" evidence="8">
        <location>
            <begin position="17"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="Hemocyanin N-terminal" evidence="10">
        <location>
            <begin position="32"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Hemocyanin middle" evidence="9">
        <location>
            <begin position="160"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Hemocyanin C-terminal" evidence="11">
        <location>
            <begin position="503"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137424.2"/>
        </source>
    </evidence>
    <sequence length="787" mass="96041" checksum="742D27348927B6BF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V342</accession>
    <name>A0A6I8V342_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC6897573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137718.1">
        <property type="nucleotide sequence ID" value="XM_002137682.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241883.1">
        <property type="nucleotide sequence ID" value="XM_033385992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V342"/>
    <dbReference type="GeneID" id="6897573"/>
    <dbReference type="KEGG" id="dpo:6897573"/>
    <dbReference type="OMA" id="KQMYEVH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248745">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF6">
        <property type="entry name" value="CHK DOMAIN OV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="144"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241883.1"/>
        </source>
    </evidence>
    <sequence length="424" mass="48706" checksum="0DEAA74648952C87" modified="2020-08-12" version="1">MSPNSSSEKPVNPNEHLDIPKWVNEEYFLPILEKDVNGFDKIVSFTPIAATAPGENYTSVMIRVHIDALLKDGSRQKISYILKTMLESSQGADAVNAMGLFPKEKQMYEVHIPQFLQLYKEAGVDIELAPKCLLVDQTAEAISLVFEDLSRQNFVNCDRLTGFDMEHMRCVLRKLAEFHAASVLVHDLNGPYDELYTKSMYNEESRPLFEKMGELRQAQYIDAMRQWGLEDVEKYIEKYWSADEFFDLALSVNAVDESDFNVLNHGDCWSNNIMFNYKENGQVDRTIFVDLQIGKWGSPAQDLWYLICTSAHLDIKIKEFDHFIQIYHERLAECLKLLNYSKPIPLLRDLHIMMLKYGFWGPLTANGVMVATLMPSDKDSNISTMMDPGPKGDAMRLKTFTNVYYVEAMKLLLPFLKLKGLLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V364</accession>
    <name>A0A6I8V364_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Sdhaf3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137753.2">
        <property type="nucleotide sequence ID" value="XM_002137717.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V364"/>
    <dbReference type="FunCoup" id="A0A6I8V364">
        <property type="interactions" value="1113"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897608"/>
    <dbReference type="InParanoid" id="A0A6I8V364"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137753.2"/>
        </source>
    </evidence>
    <sequence length="131" mass="14904" checksum="609D149174CB9A22" modified="2020-08-12" version="1">MSNLVINQLTHPQRVRLLYKTILRLHRGMPTELRALGDGYVRDEFRRHLKCNPMEAQLFMTEWARYASTITQQLGLRGKPKGELGEEMDPSAVEMLKDDQVVQLYELMLAAKGLDGDTITADDVRGSSKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3U6</accession>
    <name>A0A6I8V3U6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137498.3">
        <property type="nucleotide sequence ID" value="XM_002137462.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3U6"/>
    <dbReference type="KEGG" id="dpo:6897345"/>
    <dbReference type="InParanoid" id="A0A6I8V3U6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026229658" evidence="1">
        <location>
            <begin position="22"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137498.3"/>
        </source>
    </evidence>
    <sequence length="232" mass="26896" checksum="CF89790CE00F3953" modified="2020-08-12" version="1">MMRFVRSTLLLLSFLWVESKSRRIWDKQGPLYESTKLLIAEESFRADQLWNDTHHAIYAHAEQVQQAKDTLDTKQQQVSARLEDFFDHQYTVEWGSCFKQHEREVAHFNRELIDKYSICRQSLDSVMEHFEQEVVQEANFLNVAAQKISDLSKICQIWQLKQPSFNHAGVLLCTVAGIGGINQRMASSLELCWDILLELSLEELDTPSCTAYHQLKMQFDVVYAGIESCVMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3V6</accession>
    <name>A0A6I8V3V6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Fib</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles. It is associated with the U3, U8 and U13 small nuclear RNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138278.2">
        <property type="nucleotide sequence ID" value="XM_002138242.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3V6"/>
    <dbReference type="FunCoup" id="A0A6I8V3V6">
        <property type="interactions" value="1379"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898210"/>
    <dbReference type="InParanoid" id="A0A6I8V3V6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245878">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000056">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000001">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="8"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002138278.2"/>
        </source>
    </evidence>
    <sequence length="342" mass="35344" checksum="333D9698877CE258" modified="2020-08-12" version="1">MGKPGFSPRGGGVGRGGGGGFRGRGGGGEGRGGGGFRGRGGGDRGGSRGGFGGRGGGDRGGFGGRGGGDRGGGRGRGGFGGRGGGDRGRGGRGAGRGGGAGGFKGGKTVTIEPHRHEGVFIARGKEDALVTRNFVPGSEVYGEKRISVETGGEKIEYRVWNPFRSKLAAAILGGVEKIHMPPGSKVLYLGAASGTTVSHVSDVVGPEGLVYAVEFSHRSGRDLINVAKKRTNIIPIIEDARHPHKYRMLVGMVDTIFADVAQPDQGRIVALNAQHFLKNGGHFVISIKASCIDSTAQPEAVFAAEVKKMQADKLKPQEQLTLEPYERDHAVVVGVYRPPGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3V7</accession>
    <name>A0A6I8V3V7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sodium-coupled monocarboxylate transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">bumpel</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138067.1">
        <property type="nucleotide sequence ID" value="XM_002138031.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3V7"/>
    <dbReference type="FunCoup" id="A0A6I8V3V7">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="GeneID" id="6897968"/>
    <dbReference type="KEGG" id="dpo:6897968"/>
    <dbReference type="InParanoid" id="A0A6I8V3V7"/>
    <dbReference type="OMA" id="SIMFAIM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248935">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11492">
        <property type="entry name" value="SLC5sbd_NIS-SMVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF38">
        <property type="entry name" value="FI02016P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002138067.1"/>
        </source>
    </evidence>
    <sequence length="589" mass="64756" checksum="D2390F40E3D20B2E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3Z4</accession>
    <name>A0A6I8V3Z4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Endonuclease</fullName>
            <ecNumber evidence="14">3.1.30.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">EndoG</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.30.-" evidence="14"/>
    <dbReference type="RefSeq" id="XP_002138133.2">
        <property type="nucleotide sequence ID" value="XM_002138097.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3Z4"/>
    <dbReference type="FunCoup" id="A0A6I8V3Z4">
        <property type="interactions" value="372"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898047"/>
    <dbReference type="InParanoid" id="A0A6I8V3Z4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00091">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.570.10:FF:000002">
        <property type="entry name" value="Endonuclease G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018524">
        <property type="entry name" value="DNA/RNA_endonuclease_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF5">
        <property type="entry name" value="ENDONUCLEASE G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01070">
        <property type="entry name" value="NUCLEASE_NON_SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14 18" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="16">
        <location>
            <begin position="83"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="15">
        <location>
            <begin position="84"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002138133.2"/>
        </source>
    </evidence>
    <sequence length="304" mass="34171" checksum="B2D01A8B1E187F26" modified="2020-08-12" version="1">MSGLRVRGLLALSATALGAYYLGSRTERLQQSSDVFPRLPGLPTFGTVSAASLVASNVNLTATPSRISEIMKFGFPGLDNVRSHSDYVLSYDRRNRIPHWVFEHLTAESVAKNDSVDRSKCDFKPDESIHPFFRSQNTDYRKSGFDRGHMAAAGNHRLHQKHCEETFYLSNMAPQVGQGFNRDAWNSLEMHVRKLTKSYSNVYVCTGPLYLPHKEDDGKMYVKYEVIGSNTVAVPTHFYKVIVGEAADHKLHMEAYVMPNKVISNDTPLNVFQVPPETVERAAGLLFFDQINRNQLASINGRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4P9</accession>
    <name>A0A6I8V4P9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Gr58a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138819.2">
        <property type="nucleotide sequence ID" value="XM_002138783.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4P9"/>
    <dbReference type="FunCoup" id="A0A6I8V4P9">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805263"/>
    <dbReference type="InParanoid" id="A0A6I8V4P9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033041">
        <property type="term" value="F:sweet taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF131">
        <property type="entry name" value="GUSTATORY AND ODORANT RECEPTOR 63A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002138819.2"/>
        </source>
    </evidence>
    <sequence length="409" mass="47440" checksum="C4BCE4958F5E7872" modified="2020-08-12" version="1">MSIGLLLKTFHSYGLGSGLLPAPLRLDLDLDRIHFSKRSHQRRFYLAYTACLNVLLIVLLPCTFPVFMYDESYMRDKLVLQWTFNLTNVTRIMAMVACGYLTWTKREPLLQLGEGLARHCHRCRQLENGALHPSGYRELQKRIRGLLRQQVFVLNLSIVSGTLLLMRIDTDVRRSNIIMVVVHMLQFIYVSIMMSGLYVICLLLYWQMERVNLALKELCSRLHHEERNALLLSASLARQTLHSLGHLLQLHCEGQRLMRSLFGIFDVTIAFLLLKMFVTNVNLLYHAVQFGNDSIDTTNVTKLWGESLIVTHYWTAVLLMNLVDDLTRQNGLETGEILRQFSDLELVKREFQLELERFSDHLRCHSTAYKCCGLFVFNKQTSLIYFFSALVNVLVLYQFDLKNSVLEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V525</accession>
    <name>A0A6I8V525_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Protein CBFA2T3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nvy</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138992.3">
        <property type="nucleotide sequence ID" value="XM_002138956.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V525"/>
    <dbReference type="FunCoup" id="A0A6I8V525">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899068"/>
    <dbReference type="InParanoid" id="A0A6I8V525"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1110">
        <property type="entry name" value="TAFH/NHR1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013289">
        <property type="entry name" value="CBFA2T1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013293">
        <property type="entry name" value="Nervy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014896">
        <property type="entry name" value="NHR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037249">
        <property type="entry name" value="TAFH/NHR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003894">
        <property type="entry name" value="TAFH_NHR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379">
        <property type="entry name" value="MTG8 ETO EIGHT TWENTY ONE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379:SF14">
        <property type="entry name" value="NERVY, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08788">
        <property type="entry name" value="NHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07531">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01875">
        <property type="entry name" value="ETOFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01879">
        <property type="entry name" value="NERVYPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00549">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158553">
        <property type="entry name" value="TAFH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51119">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAFH" evidence="12">
        <location>
            <begin position="217"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="11">
        <location>
            <begin position="607"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="94"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="423"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="580"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="95"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="428"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002138992.3"/>
        </source>
    </evidence>
    <sequence length="741" mass="76837" checksum="87D0535C2A7704CA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5G7</accession>
    <name>A0A6I8V5G7_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Homeobox protein homothorax isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hth</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/MEIS homeobox family.</text>
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    </dbReference>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:animal organ morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:eye development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048663">
        <property type="term" value="P:neuron fate commitment"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="HD"/>
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        <property type="entry name" value="PKNOX/Meis_N"/>
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        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF05920">
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    </dbReference>
    <dbReference type="Pfam" id="PF16493">
        <property type="entry name" value="Meis_PKNOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="363"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="365"/>
            <end position="427"/>
        </location>
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        <location>
            <begin position="25"/>
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            <begin position="333"/>
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            <begin position="333"/>
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    </feature>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_003736365.1"/>
        </source>
    </evidence>
    <sequence length="487" mass="52829" checksum="FCDDC86861294739" modified="2020-08-12" version="1">MAQPRYDDGLHGYGMDSGAAAAAMYDPHAGHRPPGLQGLPSHHSPHMTHAAAAAATVGMHGYHSGAGGHGTPSHVSPVGNHLMGAIPEVHKRDKDAIYEHPLFPLLALIFEKCELATCTPREPGVQGGDVCSSESFNEDIAMFSKQIRSQKPYYTADPEVDSLMVQAIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDERDTTKPPELGSANGEGRSNADSTSHTDGASTPDVRPPSSSLSYGGAMNDDARSPGAGSTPGPLSQQPPVLDTSDPDGKFLSSLNPSELTYDGRWCRREWSSPADARNADASRRLYSSVFLGSPDNFGTSASGDASNASIGSGEGTGEEDDDSSGKKNQKKRGIFPKVATNILRAWLFQHLTHPYPSEDQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVYTPHPGPSGYGHDAMGYMMDSQAHMMHRPPGDPGFHQGYPHYPPAEYYGQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7I5</accession>
    <name>A0A6I8V7I5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">RNA-binding protein 39 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">Caper</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035468.2">
        <property type="nucleotide sequence ID" value="XM_015179982.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235280.1">
        <property type="nucleotide sequence ID" value="XM_033379389.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070932">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="RRM1_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12284">
        <property type="entry name" value="RRM2_RBM23_RBM39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding protein 39 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="RNA-binding protein 39 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="RBM39_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006509">
        <property type="entry name" value="RBM39_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01622">
        <property type="entry name" value="SF-CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48036">
        <property type="entry name" value="SPLICING FACTOR (PAD-1), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G15810)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15519">
        <property type="entry name" value="RBM39linker"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="266"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="364"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="86"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015035468.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033235280.1"/>
        </source>
    </evidence>
    <sequence length="624" mass="68922" checksum="E2B17D77DF2CCBD7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9T5</accession>
    <name>A0A6I8V9T5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="22 23 24">Neuronal acetylcholine receptor subunit beta-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22 23 24 25" type="primary">nAChRalpha5</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23 24" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22 23 24">MV-25-SWS-2005</strain>
            <tissue evidence="22 23 24">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015036793.2">
        <property type="nucleotide sequence ID" value="XM_015181307.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036795.2">
        <property type="nucleotide sequence ID" value="XM_015181309.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235302.1">
        <property type="nucleotide sequence ID" value="XM_033379411.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235303.1">
        <property type="nucleotide sequence ID" value="XM_033379412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9T5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V9T5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18997">
        <property type="entry name" value="LGIC_ECD_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000016">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000023">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit alpha6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 22 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="562"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="592"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="323"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="537"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="26"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="275"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_015036793.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_015036795.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_033235302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_033235303.1"/>
        </source>
    </evidence>
    <sequence length="824" mass="92104" checksum="093C59E45D618478" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9V9</accession>
    <name>A0A6I8V9V9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small ubiquitin-related modifier 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC26533335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015036269.2">
        <property type="nucleotide sequence ID" value="XM_015180783.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9V9"/>
    <dbReference type="KEGG" id="dpo:26533335"/>
    <dbReference type="InParanoid" id="A0A6I8V9V9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V9V9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd01763">
        <property type="entry name" value="Ubl_SUMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036269.2"/>
        </source>
    </evidence>
    <sequence length="115" mass="13425" checksum="26AEAA9F39A6AAFD" modified="2020-08-12" version="1">MEQIRIKVNAFHHQLHVYAFTEGQLEMTQNFTQFKNIYCKELDLPLKRLRFLFNGQFIGDTETPSDLGMEEGDEIDIFTAKEGPGLSEFIDNQFKKFLIQKCRPFNSQGVWESNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAW0</accession>
    <name>A0A6I8VAW0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7 8">Uncharacterized protein Apc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8 9" type="primary">Apc</name>
    </gene>
    <organism evidence="5 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7 8">MV-25-SWS-2005</strain>
            <strain evidence="5">MV2-25</strain>
            <tissue evidence="6 7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenomatous polyposis coli (APC) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358709.3">
        <property type="nucleotide sequence ID" value="XM_001358672.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037344.2">
        <property type="nucleotide sequence ID" value="XM_015181858.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037345.2">
        <property type="nucleotide sequence ID" value="XM_015181859.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037346.2">
        <property type="nucleotide sequence ID" value="XM_015181860.2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801657"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030877">
        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000305">
        <property type="entry name" value="Adenomatous polyposis coli"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009240">
        <property type="entry name" value="APC_15aa_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026818">
        <property type="entry name" value="Apc_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041257">
        <property type="entry name" value="APC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009223">
        <property type="entry name" value="APC_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12607">
        <property type="entry name" value="ADENOMATOUS POLYPOSIS COLI PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12607:SF12">
        <property type="entry name" value="APC-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05972">
        <property type="entry name" value="APC_15aa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05923">
        <property type="entry name" value="APC_r"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18797">
        <property type="entry name" value="APC_rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="repeat" description="ARM" evidence="3">
        <location>
            <begin position="266"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="3">
        <location>
            <begin position="510"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="144"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="668"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="866"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1027"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1168"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1277"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1329"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1430"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1519"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1622"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1665"/>
            <end position="1691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1976"/>
            <end position="2015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2123"/>
            <end position="2150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2187"/>
            <end position="2365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="2380"/>
            <end position="2442"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="866"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="876"/>
            <end position="889"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1036"/>
            <end position="1054"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1336"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1452"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1519"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1542"/>
            <end position="1561"/>
        </location>
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        <location>
            <begin position="1625"/>
            <end position="1637"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1667"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1678"/>
            <end position="1691"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1986"/>
            <end position="2010"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="2123"/>
            <end position="2133"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="2190"/>
            <end position="2202"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="2231"/>
            <end position="2242"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="2243"/>
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        <location>
            <begin position="2339"/>
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        <location>
            <begin position="2384"/>
            <end position="2401"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="2414"/>
            <end position="2425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358709.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015037344.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015037345.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015037346.2"/>
        </source>
    </evidence>
    <sequence length="2442" mass="263372" checksum="1E07759E7D4A6728" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VB64</accession>
    <name>A0A6I8VB64_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein yellow</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">yellow-e2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major royal jelly protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037524.1">
        <property type="nucleotide sequence ID" value="XM_015182038.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VB64"/>
    <dbReference type="FunCoup" id="A0A6I8VB64">
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    </dbReference>
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    <dbReference type="KEGG" id="dpo:4801990"/>
    <dbReference type="InParanoid" id="A0A6I8VB64"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000045">
        <property type="entry name" value="Blast:Protein yellow"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017996">
        <property type="entry name" value="MRJP/yellow-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009:SF7">
        <property type="entry name" value="GH10609P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10009">
        <property type="entry name" value="PROTEIN YELLOW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03022">
        <property type="entry name" value="MRJP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01366">
        <property type="entry name" value="ROYALJELLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026163716" evidence="6">
        <location>
            <begin position="18"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015037524.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="49510" checksum="344B29C328E02EDF" modified="2020-08-12" version="1">MNILPFFLLALLSLTTAQYYTHRPKPSQPAFEHDQQYKKLHRVYEWKNIQYGFPSEREREEVLRSGRYNPDSPIPIDIDVYYPPQGGAVRHFITTPRFGQGVPYSLAYVTQVQRENGSEIQAYPSYDWHKTHGGDCDGLTSVYRVHIDACGQMWILDSGEIEFVQHCAPQVVVIDLATNQLIHRYRLPEDTFKAKVSRFVTILADIQDPPPQGVCKDAYVYMADPTSKAIVVYDVKGNRSWRVENKFTYPDARFGTLTVAGESFELLDGALALAITPNGLGLRRHLIFHALSNELELAIPLDVLQNSTRWQQGISSSLQEFVTLGRRGVQCAAHAISRRGFWFCGFLEPIGIFGWNIQTPYARPNLQLLALNPDTLQFVSGMKIVTRPSDGQEELWLLSNRLQKVFGGTIDYKEINYRVQRCDVDDMLQGRGCAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VE74</accession>
    <name>A0A6I8VE74_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Diuretic hormone receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 19" type="primary">Dh44-R1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <strain evidence="17">MV2-25</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for the insect diurectic hormone. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015039408.1">
        <property type="nucleotide sequence ID" value="XM_015183922.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039409.1">
        <property type="nucleotide sequence ID" value="XM_015183923.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VE74"/>
    <dbReference type="GeneID" id="4803884"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081055">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VE74">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008036">
        <property type="term" value="F:diuretic hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15263">
        <property type="entry name" value="7tmB1_DH_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000155">
        <property type="entry name" value="diuretic hormone receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000023">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002001">
        <property type="entry name" value="GPCR_2_diuretic_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF15">
        <property type="entry name" value="DIURETIC HORMONE 44 RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01127">
        <property type="entry name" value="DIUHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="15">
        <location>
            <begin position="38"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="16">
        <location>
            <begin position="133"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="410"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015039408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015039409.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="56576" checksum="98FE0BB5ADA4A402" modified="2020-08-12" version="1">MSDHNYMEAAVAANGSGGIDPLLMLSNLDGIGASVELQCLVQEHMDSTTYGNASDHCLTQFDSILCWPRTARGTLAVLQCMDELQGIHYDSSKNATRFCHANGTWEKYTNYDACAHLPASHSVTEFEVIVELPTIIYYIGYALSLVSLSLALIVFAYFKELRCLRNTIHANLFFTYIMSALFWILLLSVQISIRSGQASCIALVTLFHFFTLTNFFWMLVEGLYLYMLVVNTFSGDNIRFNIYASIGWGGPALFVITWLVAKSLTVSYNSSEKYDINCPWMEETNVDWIFQGPVCAVLLINLTFLLRIMWVLITKLRSANTVETRQYRKAAKALLVLIPLFGITYLVVLAGPSEVGLMGHMFAVLRAVLLSTQGFSVSLFYCFLNSEVRNALRHHISTWRDTRDIQQSQNRRYTTKSFSKGGSSPRAESMRPLTSYYGRGKRESCVSSATTTTLVGQHPPCLALHRGSNNALHTGGGNTTCSGSTLSVMPRAISPLMKLQQGLEENSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VEX4</accession>
    <name>A0A6I8VEX4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Calbindin-32 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Cbp53E</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calbindin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015039669.1">
        <property type="nucleotide sequence ID" value="XM_015184183.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VEX4"/>
    <dbReference type="SMR" id="A0A6I8VEX4"/>
    <dbReference type="FunCoup" id="A0A6I8VEX4">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="GeneID" id="4804190"/>
    <dbReference type="InParanoid" id="A0A6I8VEX4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079791">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900271">
        <property type="term" value="P:regulation of long-term synaptic potentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099509">
        <property type="term" value="P:regulation of presynaptic cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16179">
        <property type="entry name" value="EFh_HEF_CBN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000054">
        <property type="entry name" value="Calbindin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000262">
        <property type="entry name" value="calbindin-32 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000304">
        <property type="entry name" value="calbindin-32 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051001">
        <property type="entry name" value="Calbindin_Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035799">
        <property type="entry name" value="EFh_CBN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972:SF10">
        <property type="entry name" value="CALBINDIN-32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="84"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="228"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015039669.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="36166" checksum="E558D9C7784E6297" modified="2020-08-12" version="1">MDSAAAAAAKRVQIEKAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVASANVTDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKEGIDGATKLTKDDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGHDKHGKISRKELTMILLTLAKISPDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VF80</accession>
    <name>A0A6I8VF80_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC4804558</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361100.3">
        <property type="nucleotide sequence ID" value="XM_001361063.4"/>
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        <property type="nucleotide sequence ID" value="XM_015184523.2"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038875">
        <property type="entry name" value="PLA2_conodipine-like"/>
    </dbReference>
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        <property type="entry name" value="AGAP006772-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AGAP006772-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634512" evidence="2">
        <location>
            <begin position="24"/>
            <end position="805"/>
        </location>
    </feature>
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        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="546"/>
        </location>
    </feature>
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        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
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        <location>
            <begin position="413"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361100.3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015040009.2"/>
        </source>
    </evidence>
    <sequence length="805" mass="92069" checksum="BAC838257124736F" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8VFG2</accession>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
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        <name evidence="3 4" type="primary">LOC4804750</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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                <consortium name="RefSeq"/>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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            <begin position="92"/>
            <end position="114"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361262.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015040178.2"/>
        </source>
    </evidence>
    <sequence length="140" mass="15792" checksum="5ACC8B15AD82C33D" modified="2020-08-12" version="1">MAEDPLNQQAFAQDVEAAAAAGGPNGFAGAEPGHPDFKMPSADEIWKLVESMDGISDAEREELRESIFNPKPPSPEDFMRQYGQPGHSSREYMIFFVMLTLILFVFALFGYKLYKSLTEKELKKQEKLKSKQQKKAKKSN</sequence>
</entry>
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    <accession>A0A6I8VHZ2</accession>
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        <submittedName>
            <fullName evidence="6 7">Retinol dehydrogenase 12</fullName>
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        <name evidence="6 7" type="primary">LOC4815628</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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            <strain evidence="6 7">MV-25-SWS-2005</strain>
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    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
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            <begin position="353"/>
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        </location>
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        <location>
            <begin position="362"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001355606.3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015041293.2"/>
        </source>
    </evidence>
    <sequence length="394" mass="43150" checksum="3B453C22E7FC8DE5" modified="2020-08-12" version="1">MSEAVTPIGGTGTTALPAGFDPTAEAVEKTLCFRGFWAWFVLLLLIGICALFVMWLLRKCIQGPAYRKANRIDGKVVIVTGCNTGIGKETVLELARRGAKVYMACRDPGRCEAARIEIMDRTQNQQLFNRSLDLGSLESVRNFVARFKAEESRLDLLINNAGIMACPRSLTADGYEQQLGVNHLGHFLLTNLLLDRLKQSTPSRIVVVSSAAHLFGRINREDLMSERKYSKFFGAYSQSKLANILFTRKLSVLLKDTGVTVNCCHPGVVRTELNRHFAGPAWMKSALQVVSLYFFKTPRAGAQTTLRLALDPSLESSTGGYYSDSMRFPLVPWARSTDTADWLWRESEKLVGLPPIEPPPAQNGNGSTNGSANGNGSPGTAAAETVDTVVVNRS</sequence>
</entry>
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    <accession>A0A6I8VIB9</accession>
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        <submittedName>
            <fullName evidence="8">Broad-complex core protein isoforms 1/2/3/4/5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6901470</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015041732.2">
        <property type="nucleotide sequence ID" value="XM_015186246.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8VIB9">
        <property type="interactions" value="377"/>
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    </dbReference>
    <dbReference type="Bgee" id="FBgn0247847">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CG12236"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF106">
        <property type="entry name" value="FI01104P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
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    </dbReference>
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    <sequence length="532" mass="59023" checksum="40D500238928ECEC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="18">MV-25-SWS-2005</strain>
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            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        </reaction>
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        <cofactor evidence="1">
            <name>Mg(2+)</name>
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        <subcellularLocation>
            <location evidence="2">Nucleus</location>
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            <position position="1041"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="3">LOW QUALITY PROTEIN: uncharacterized protein</fullName>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">MV2-25</strain>
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                <consortium name="RefSeq"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232659.1"/>
        </source>
    </evidence>
    <sequence length="73" mass="8200" checksum="FAA6A17BE5D6C9BD" modified="2020-08-12" version="1">MIRKQFKLLFVFDATTTSNQEPPTCLPNGSKSNWPTKNEPEWGQCNEHTSWTTVAFGHWDTTGSTIDGSPKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQP5</accession>
    <name>A0A6I8VQP5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">LOW QUALITY PROTEIN: forkhead box protein N2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6899343</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033233400.1">
        <property type="nucleotide sequence ID" value="XM_033377509.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899343"/>
    <dbReference type="InParanoid" id="A0A6I8VQP5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VQP5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20030">
        <property type="entry name" value="FH_FOXN1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049624">
        <property type="entry name" value="FOXN1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46721:SF3">
        <property type="entry name" value="FORKHEAD BOX N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46721">
        <property type="entry name" value="FORKHEAD BOX PROTEIN N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="152"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="152"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="83"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033233400.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="48391" checksum="DF046E707525FD8C" modified="2020-08-12" version="1">SQSSPSEEGGNKTSKTSSNLLTTGDVSTNTIKLAPGIGGLTFSNSAAYQKLKHQSSAKSINGSSSSSDNGMVLKXSKRGLLQQGSTTPKSIASAAHSPHHQMQSMGSTSSGSSLASSSPLSNGSNNSSGSAGLVKPLQQKVKLPVVGSALPKPAYSYSCLIALALKNSRAGSLPVLEIYSFLCQHYPYFENAPSGWKNSVRHNLSLNKCFEKIERPATNGNQRNGCRWALNPERITKMDEEVQKWSRKDPQAIRCAMVYPEHLESLERGEMKHGSADSDVELDSTSEIEESSDLEEQDFDGTLVDAMLVEEDEDVVDEDEDDDMLGDIEVSLERHLVEKSSLLYEKADEQLYDAIDIEDDKEAVRRAIGSGQGTQIIELNPANLNANHNGYHHQQQKVKRARLDINYAIGPAGEQQYGQKVKVQQLVQTQAQPQEQLIVVVFAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQX7</accession>
    <name>A0A6I8VQX7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898651</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233475.1">
        <property type="nucleotide sequence ID" value="XM_033377584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQX7"/>
    <dbReference type="KEGG" id="dpo:6898651"/>
    <dbReference type="InParanoid" id="A0A6I8VQX7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VQX7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21112">
        <property type="entry name" value="CHEMOSENSORY PROTEIN A 29A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21112:SF0">
        <property type="entry name" value="CHEMOSENSORY PROTEIN A 29A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233475.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="18391" checksum="2079D83D8F019D9D" modified="2020-08-12" version="1">MTSSLSTFVLTKWDYEPVSIETYSTDESQLKIESKIDRLKRGEFGISCTLEWIYDIDETTMVEGTAYRSSSGAENDYKILPWSVPKLPLYEYLDTYYKDLLIPNIGHCSNLPQFKGKFQPPWPKQIYHLDKCVFDGEGLPEVVPPGFYKIIFSLNGFYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSU1</accession>
    <name>A0A6I8VSU1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC6898442</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234121.1">
        <property type="nucleotide sequence ID" value="XM_033378230.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234122.1">
        <property type="nucleotide sequence ID" value="XM_033378231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VSU1"/>
    <dbReference type="KEGG" id="dpo:6898442"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VSU1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634553" evidence="1">
        <location>
            <begin position="19"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234122.1"/>
        </source>
    </evidence>
    <sequence length="367" mass="40604" checksum="49521558149F6F46" modified="2020-08-12" version="1">MNISALCVCLQWLQMTVSPDVTGHSQKQPLFHGYSVLSTFFAFTVKVEPQSLQRCRNDQRRPLAQLWPTTPCLSHQKTNFIGKQLKKEEKEQTLDGIWTPGSSGFWRLVRWKDVGNGACSRVGPMRRLISSRSPAWMAAVVLCGRGVEKPRSLVLETRRGCMYLNRVASDSISRATRFPCIRTTCTGATGAPGTLIKTGQSRSLAKDVACIPPSYDGLPELAAAGVRQDYPFLGELAEASKRLLFWTDISSHQAIIRARVDGNEHVDLEEVTTLALDQQSDIPCVSIKIRASMHISQVSAFGRFVSDDKTGLERSAITPVCTVAQSVSTSAWPAGYDTQYSRRMLVPDSDALMLLEGKENYGVYPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VV74</accession>
    <name>A0A6I8VV74_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6903739</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_033234961.1">
        <property type="nucleotide sequence ID" value="XM_033379070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VV74"/>
    <dbReference type="KEGG" id="dpo:6903739"/>
    <dbReference type="InParanoid" id="A0A6I8VV74"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VV74">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000011">
        <property type="entry name" value="Prolyl alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026150256" description="procollagen-proline 4-dioxygenase" evidence="13">
        <location>
            <begin position="21"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="400"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033234961.1"/>
        </source>
    </evidence>
    <sequence length="517" mass="59949" checksum="84EB143A0E2B85BE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXR5</accession>
    <name>A0A6I8VXR5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histone H1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117183919</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235359.1">
        <property type="nucleotide sequence ID" value="XM_033379468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXR5"/>
    <dbReference type="KEGG" id="dpo:117183919"/>
    <dbReference type="InParanoid" id="A0A6I8VXR5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235359.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="18646" checksum="497D234D893B1963" modified="2020-08-12" version="1">MATAAQHTPTQTTTRQYIRNLFENISLLFFWYLFFVFLSASAKKAPNPKVASVEKKVKSKKVASSSSAATKKTASPKKAAAAGDKKLKVKKSVATKKTAEKKKTEKAKAKDAKKTGIVKAKPAATKAKSSPAKPKPKPKAKAVAAAASVKPKKTTKKPAAPATVKKPKAKTTAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYR0</accession>
    <name>A0A6I8VYR0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NHL repeat-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117184108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236202.1">
        <property type="nucleotide sequence ID" value="XM_033380311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYR0"/>
    <dbReference type="FunCoup" id="A0A6I8VYR0">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184108"/>
    <dbReference type="InParanoid" id="A0A6I8VYR0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd14951">
        <property type="entry name" value="NHL-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03012">
        <property type="entry name" value="TlpA_like_DipZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045302">
        <property type="entry name" value="NHL2_NHL_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46388">
        <property type="entry name" value="NHL REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46388:SF2">
        <property type="entry name" value="NHL REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="49"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="2">
        <location>
            <begin position="482"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="2">
        <location>
            <begin position="526"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033236202.1"/>
        </source>
    </evidence>
    <sequence length="722" mass="80103" checksum="5F0775D7ED6EF113" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0U3</accession>
    <name>A0A6I8W0U3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16 17">Uncharacterized protein Rim isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16 17" type="primary">Rim</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15 16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15 16 17">MV-25-SWS-2005</strain>
            <strain evidence="14">MV2-25</strain>
            <tissue evidence="15 16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033236947.1">
        <property type="nucleotide sequence ID" value="XM_033381056.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236952.1">
        <property type="nucleotide sequence ID" value="XM_033381061.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236957.1">
        <property type="nucleotide sequence ID" value="XM_033381066.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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        <scope>IDENTIFICATION</scope>
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        <property type="term" value="F:carboxypeptidase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008241">
        <property type="term" value="F:peptidyl-dipeptidase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd06461">
        <property type="entry name" value="M2_ACE"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M2"/>
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    <dbReference type="PANTHER" id="PTHR10514">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME"/>
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    </dbReference>
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        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01401">
        <property type="entry name" value="Peptidase_M2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00791">
        <property type="entry name" value="PEPDIPTASEA"/>
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        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS52011">
        <property type="entry name" value="PEPTIDASE_M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6 13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634588" description="Angiotensin-converting enzyme" evidence="14">
        <location>
            <begin position="26"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor 1" evidence="5">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor 2" evidence="7">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor 1" evidence="5">
        <location>
            <position position="508"/>
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    <feature type="active site" description="Proton donor 2" evidence="7">
        <location>
            <position position="508"/>
        </location>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
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    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <note>catalytic</note>
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    </feature>
    <feature type="binding site" evidence="11">
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            <position position="378"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="382"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
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        <location>
            <position position="406"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <position position="517"/>
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        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
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        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; partial" evidence="6">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine; partial" evidence="6">
        <location>
            <position position="333"/>
        </location>
    </feature>
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        <location>
            <begin position="145"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10 12">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10 12">
        <location>
            <begin position="533"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU361144"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015035319.2"/>
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    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033236982.1"/>
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    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033236983.1"/>
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    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033236984.1"/>
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    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033236985.1"/>
        </source>
    </evidence>
    <sequence length="633" mass="73771" checksum="33559022C277D7AC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1Z7</accession>
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        <name evidence="12" type="primary">DIP-iota</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="12">MV-25-SWS-2005</strain>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="CLUMA_CG000981, isoform A"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
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    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
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        <location>
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            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_033236709.1"/>
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    <sequence length="397" mass="45232" checksum="8306B98DD0DF4AC6" modified="2020-08-12" version="1">MRKTFADVWLLLHCVCLSRASFSELNNSDPKFSGPINNVTSPVGREALLTCVVHELVSFKVAWLRVDTQTILSIQNHVITKNHRIAISHTEHRIWQLKIRDVQESDRGWYMCQINTDPMKSQMGYLDVVVPPDIVDFQTSQDVVRATGQNVTLTCYATGVPTPTITWRREESTPLWLTDEGDREVYSVEGQNLTLWQVQRTHMGAYLCIASNGVPPTVSKRVMLVVNFAPTIWTRYDTIYVGLGQKVTLECITESQPASVNFWLKDTELLQGGSYESVAVDHAYRIVMRITLRPVTKRDFGEYKCIAKNALGETERSITVHRKSVRSTYNDLYLTQSSLSVITDKAKKHGQHSHQTSAHESQFIVIEEYLANMAHRFCSFHSVWLILLCFVNKVSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W203</accession>
    <name>A0A6I8W203_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC117184392</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033381489.1"/>
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    <dbReference type="KEGG" id="dpo:117184392"/>
    <dbReference type="InParanoid" id="A0A6I8W203"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026310592" evidence="2">
        <location>
            <begin position="19"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033237380.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="16412" checksum="96EEBC6148535F57" modified="2020-08-12" version="1">MEKITLFALICLIGTAVATPMGFTHATTSPKQAGTAPGVVHWTTGVQTKGSTALNQTKTTTNGKPTVQAVKVTATTAALPVVHNSSAVQGSTTTVDTTLPAASDTKTQAPNEATTPPPAHSEETVTTTEAATTVAVTEQIIQKEAQSAKQKQRKAKKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W2F3</accession>
    <name>A0A6I8W2F3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tumor susceptibility gene 101 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TSG101</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033237546.1">
        <property type="nucleotide sequence ID" value="XM_033381655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W2F3"/>
    <dbReference type="FunCoup" id="A0A6I8W2F3">
        <property type="interactions" value="907"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900149"/>
    <dbReference type="InParanoid" id="A0A6I8W2F3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11685">
        <property type="entry name" value="UEV_TSG101-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052070">
        <property type="entry name" value="ESCRT-I_UEV_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017916">
        <property type="entry name" value="SB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008883">
        <property type="entry name" value="UEV_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306:SF3">
        <property type="entry name" value="TUMOR SUPPRESSOR PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306">
        <property type="entry name" value="TUMOR SUSCEPTIBILITY GENE 101 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05743">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09454">
        <property type="entry name" value="Vps23_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51312">
        <property type="entry name" value="SB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51322">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UEV" evidence="10">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="SB" evidence="9">
        <location>
            <begin position="342"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="260"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033237546.1"/>
        </source>
    </evidence>
    <sequence length="408" mass="45531" checksum="94E9EFA6AD743F97" modified="2020-08-12" version="1">MPAVEEAKIIKFLSKYKYVDVTKKDVIDVVTTYRSLTYDLQKFVFNDGSYKDLFTLQGTIPVVYKNNTYYIPICIWLMDTHPQNAPMCFVKPTPNMHIKVSMYVDHNGKVYLPYLHDWQPNSSDLLSLIQVMIVTFGDHPPVYSNPKEQQMAMPYPPNAYMQQSGGAGASNSFLPYPTASGASGGNFPPYPTGANFGPYPPGGSGGNAAGYPPYMNFPQQPGSYPPAGGYNPSAIPNSTGTITEEHIKASLISAVEDKLRRRIQEKVNQYQAEIETLNRTKQELVEGSTKIDSIISRLEREQVDLQKNINVLRDKEQELEKSLETLENAEAIDPDEAVTTTAPLYRQLLNAYADEAATEDAIYYLGEGLRGGVIDLETFLKHVRTLSRKQFILRATMQKCRQKAGLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W2R6</accession>
    <name>A0A6I8W2R6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Selenoprotein BthD isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">BthD</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237656.1">
        <property type="nucleotide sequence ID" value="XM_033381765.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8W2R6">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W2R6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W2R6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052674">
        <property type="entry name" value="SelWTH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33638">
        <property type="entry name" value="SELENOPROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33638:SF1">
        <property type="entry name" value="SELENOPROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0711">Selenium</keyword>
    <keyword evidence="3" id="KW-0712">Selenocysteine</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="3">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033237656.1"/>
        </source>
    </evidence>
    <sequence length="194" mass="22114" checksum="16E2C0ADE420AD02" modified="2020-08-12" version="1">MPPKRRKKVIVSREATFVPDPGAAALDPSQPVLYIEHCRSURVFGRRAEELHAALQERGLQQLQLQLNVEGTPRRGAFELHLAKQPTTEASEQHFLWSGLKRTPRAQKFPPVDDVFRWIVEMLGQVMEKQQPGQAGDQIPKEEEPEPELKQEAVPAKKSQKRKRSAKTTKSEPEPVNVPNEEQVVSTNNAKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3I9</accession>
    <name>A0A6I8W3I9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18 19">Syntaxin-binding protein 5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18 19" type="primary">Tomosyn</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17 18 19">MV-25-SWS-2005</strain>
            <tissue evidence="17 18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033237910.1">
        <property type="nucleotide sequence ID" value="XM_033382019.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237911.1">
        <property type="nucleotide sequence ID" value="XM_033382020.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237912.1">
        <property type="nucleotide sequence ID" value="XM_033382021.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902025"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15873">
        <property type="entry name" value="R-SNARE_STXBP5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003581">
        <property type="entry name" value="Lethal(2) giant larvae protein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000383">
        <property type="entry name" value="Syntaxin-binding protein 5 isoform b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000670">
        <property type="entry name" value="Tomosyn, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000664">
        <property type="entry name" value="Lethal2_giant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013577">
        <property type="entry name" value="LLGL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08366">
        <property type="entry name" value="LLGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00962">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
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    <keyword evidence="8" id="KW-0853">WD repeat</keyword>
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        <location>
            <begin position="1464"/>
            <end position="1524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="651"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="711"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="900"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="958"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1147"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="672"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="908"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="986"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1156"/>
            <end position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033237911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033237912.1"/>
        </source>
    </evidence>
    <sequence length="1529" mass="164464" checksum="DC617ED06EFDA678" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4C0</accession>
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        <submittedName>
            <fullName evidence="10">Chitin-binding domain protein cbd-1</fullName>
        </submittedName>
    </protein>
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        <name evidence="10" type="primary">LOC4813254</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033382277.1"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
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        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
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        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
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    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
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        </location>
    </feature>
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        <location>
            <begin position="49"/>
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    <feature type="domain" description="Chitin-binding type-2" evidence="8">
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    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_033238168.1"/>
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    </evidence>
    <sequence length="513" mass="55339" checksum="AF8548B0A4014986" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="12">2',5'-phosphodiesterase 12</fullName>
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        <alternativeName>
            <fullName evidence="13">Mitochondrial deadenylase</fullName>
        </alternativeName>
    </protein>
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        <name evidence="16" type="primary">LOC26532603</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033238533.1">
        <property type="nucleotide sequence ID" value="XM_033382642.1"/>
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        <property type="interactions" value="2552"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
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    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF37">
        <property type="entry name" value="2',5'-PHOSPHODIESTERASE 12"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNase I-like"/>
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    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="14">
        <location>
            <begin position="276"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083541"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033238533.1"/>
        </source>
    </evidence>
    <sequence length="600" mass="69245" checksum="FAC7E69503BF147D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5R1</accession>
    <name>A0A6I8W5R1_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18 19 20" type="primary">Ptp4E</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17 18 19">MV-25-SWS-2005</strain>
            <tissue evidence="17 18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033238014.1">
        <property type="nucleotide sequence ID" value="XM_033382123.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238015.1">
        <property type="nucleotide sequence ID" value="XM_033382124.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238016.1">
        <property type="nucleotide sequence ID" value="XM_033382125.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238017.1">
        <property type="nucleotide sequence ID" value="XM_033382126.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="CDD" id="cd14548">
        <property type="entry name" value="R3-PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000916">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001177">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000009">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000823">
        <property type="entry name" value="Tyrosine-protein phosphatase 10D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001445">
        <property type="entry name" value="Tyrosine-protein phosphatase 10D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001507">
        <property type="entry name" value="tyrosine-protein phosphatase 10D isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001461">
        <property type="entry name" value="tyrosine-protein phosphatase 10D isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF3">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1284"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="147"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="240"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="426"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="532"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="619"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="705"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="910"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="1022"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="1359"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="1531"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="814"/>
            <end position="835"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="982"/>
            <end position="1008"/>
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    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1709"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="982"/>
            <end position="994"/>
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    </feature>
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        <location>
            <begin position="1709"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1731"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1763"/>
            <end position="1776"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1777"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1794"/>
            <end position="1804"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00051722"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="17">
        <source>
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    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033238015.1"/>
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        <source>
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    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033238017.1"/>
        </source>
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    <sequence length="1810" mass="203918" checksum="89BBF8973EB7CE7E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W688</accession>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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            <begin position="227"/>
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        <source>
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    <sequence length="1202" mass="124606" checksum="BDBAF27CE0BAB9FB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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</entry>
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</entry>
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            <begin position="16"/>
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            <begin position="49"/>
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            <begin position="131"/>
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            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
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            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240115.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="25011" checksum="48574290773B25F3" modified="2020-08-12" version="1">MERKVRTCRGRPPPTVAIAATAAPPQHKRRRTKERSEQLPSMPVLIDQESFQPEPSSPTHRHRPKKKKAKKHSRRDKSGTHRRHSRHSKPEEEEEQEERHDGEEERKDMLQTEELVGNVSILEQLLTDKPRQHRRHRREKKTQPKQYHRHRRQDAPSSTSTVSTSTSPSTATSPTSTVRPRHWFGQKKRILEQWRREHSQQQQQQTRGDSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAN3</accession>
    <name>A0A6I8WAN3_DROPS</name>
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        <submittedName>
            <fullName evidence="5 6 7">Mediator of RNA polymerase II transcription subunit 12 isoform X1</fullName>
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    <gene>
        <name evidence="5 6 7" type="primary">Spt20</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT20 family.</text>
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    <dbReference type="RefSeq" id="XP_002135560.2">
        <property type="nucleotide sequence ID" value="XM_002135524.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043996.2">
        <property type="nucleotide sequence ID" value="XM_015188510.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240466.1">
        <property type="nucleotide sequence ID" value="XM_033384575.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900231"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="baseline"/>
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    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021950">
        <property type="entry name" value="Spt20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="3">
        <location>
            <begin position="382"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="881"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1107"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1158"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1227"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1460"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1522"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="1597"/>
            <end position="1623"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1643"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1912"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="46"/>
            <end position="72"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="139"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="155"/>
            <end position="172"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="196"/>
            <end position="220"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="273"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="650"/>
            <end position="669"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="881"/>
            <end position="915"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1166"/>
            <end position="1178"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1181"/>
            <end position="1194"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1195"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1231"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1522"/>
            <end position="1532"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1540"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1597"/>
            <end position="1609"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1610"/>
            <end position="1621"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1643"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1661"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1681"/>
            <end position="1702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002135560.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015043996.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033240466.1"/>
        </source>
    </evidence>
    <sequence length="1932" mass="207236" checksum="74E0E6CA7E4BC9DD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBV9</accession>
    <name>A0A6I8WBV9_DROPS</name>
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        <submittedName>
            <fullName evidence="11">Cyclin-dependent kinase 11.1 isoform X2</fullName>
        </submittedName>
    </protein>
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        <name evidence="11" type="primary">sgg</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
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    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
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        <property type="nucleotide sequence ID" value="XM_033384861.1"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
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    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="427"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="242"/>
            <end position="278"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="305"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="771"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="898"/>
            <end position="977"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="67"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="267"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="783"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="821"/>
            <end position="845"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="846"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="898"/>
            <end position="919"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="931"/>
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        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="457"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033240752.1"/>
        </source>
    </evidence>
    <sequence length="977" mass="102819" checksum="B459DC53CE3CB048" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDQ6</accession>
    <name>A0A6I8WDQ6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117185406</name>
        <name evidence="3" type="synonym">LOC4812868</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241068.1">
        <property type="nucleotide sequence ID" value="XM_033385177.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241550.1">
        <property type="nucleotide sequence ID" value="XM_033385659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDQ6"/>
    <dbReference type="KEGG" id="dpo:117185406"/>
    <dbReference type="KEGG" id="dpo:4812868"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF48">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="120"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033241068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241550.1"/>
        </source>
    </evidence>
    <sequence length="439" mass="50244" checksum="8BA6A8A53DC41C3F" modified="2020-08-12" version="1">MTESDCELSSAVSNALLALYQRQDVSLVAQPIAGVGENAYGQVLLVSWPTESKAKRVVVKMAPKNEARRQHMHVVDYYAREVFMYQNVFHTYRELADDHSAFTVAPELLASGLQAPDEFLIFEDLSQNGFQTNPRSSMPTYDIVVSTLKAVAELHACSFILQQRNQVKFRELVDHVRQDNLFTANIETVTIEFGKAMLRRTRLMLEQDEARDDDTTPMMEVLERCERNLKALTLYCVDGASQSPHAVICHGDFWNNNILYRHETHSDLAVEAKLIDFQMSRFAPPVLDLVHYLFACTEKQLRDEHLPAFLNIYYDTLSRKLGSCDLNADDIYPRTVFDRQLQLFGVFGLIMAAFSLPFFISNANEVVNIDEVSEAIQDLSASATDAPKCRELVEEFDMLNERTLPIFKRRMTDVVRDLLKYDMTPPLYQFDSTRDDDIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDT9</accession>
    <name>A0A6I8WDT9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="8">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">Edem1</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2. Seems to recognize and bind to exposed hydrophobic regions in target proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_033240728.1">
        <property type="nucleotide sequence ID" value="XM_033384837.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240729.1">
        <property type="nucleotide sequence ID" value="XM_033384838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDT9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WDT9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904154">
        <property type="term" value="P:positive regulation of retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000015">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF6">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634641" description="alpha-1,2-Mannosidase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="532"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="544"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033240728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033240729.1"/>
        </source>
    </evidence>
    <sequence length="972" mass="110987" checksum="49A1EDF1AE9D7602" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEC8</accession>
    <name>A0A6I8WEC8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Irc</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241775.1">
        <property type="nucleotide sequence ID" value="XM_033385884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEC8"/>
    <dbReference type="FunCoup" id="A0A6I8WEC8">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802089"/>
    <dbReference type="InParanoid" id="A0A6I8WEC8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF125">
        <property type="entry name" value="GH11385P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026107827" evidence="2">
        <location>
            <begin position="19"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241775.1"/>
        </source>
    </evidence>
    <sequence length="701" mass="79030" checksum="F37C880A8C0DC9EE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>B5DH54</accession>
    <accession>A0A6I8UWX1</accession>
    <name>B5DH54_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein TsetseEP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6903346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132227.1">
        <property type="nucleotide sequence ID" value="XM_002132191.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DH54"/>
    <dbReference type="STRING" id="46245.B5DH54"/>
    <dbReference type="GeneID" id="6903346"/>
    <dbReference type="KEGG" id="dpo:6903346"/>
    <dbReference type="HOGENOM" id="CLU_1469721_0_0_1"/>
    <dbReference type="InParanoid" id="B5DH54"/>
    <dbReference type="OMA" id="LEPMGYP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246702">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043511903" evidence="2">
        <location>
            <begin position="24"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002132227.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="18663" checksum="9232BA988B67BAAB" modified="2008-10-14" version="1">MKLLTVFAFICVGTFAVLHGVYGSPVPSPAPEPSPDADPKPKADPEPEPEPSPDPEPLPEPAPEPLPEPAPEPLPEPLPEPAPSPVAEPSPNPVAEPNPNPSPVASPMMPQPMLLSDKLDANFVALSASPDPAPAPAPAPAPAPVPAPFPAPLPAPSAVALEPMGYPGSLDDLVPDSIAEALPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>B5DH81</accession>
    <accession>A0A6I8UWV5</accession>
    <name>B5DH81_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Cks30A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CKS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132254.1">
        <property type="nucleotide sequence ID" value="XM_002132218.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DH81"/>
    <dbReference type="SMR" id="B5DH81"/>
    <dbReference type="FunCoup" id="B5DH81">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DH81"/>
    <dbReference type="GeneID" id="6903372"/>
    <dbReference type="KEGG" id="dpo:6903372"/>
    <dbReference type="eggNOG" id="KOG3484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140546_2_0_1"/>
    <dbReference type="InParanoid" id="B5DH81"/>
    <dbReference type="OMA" id="MSENEWR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246686">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000001">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00944">
        <property type="entry name" value="CKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002132254.1"/>
        </source>
    </evidence>
    <sequence length="75" mass="9293" checksum="87BB08792A562EB7" modified="2008-10-14" version="1">MSKDIYYSDKYYDEKFEYRHVVLPKELVKLVPKTHLMTETEWRSIGVQQSRGWIHYMIHKPEPHILLFRRPKMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKI8</accession>
    <accession>A0A6I8UYM4</accession>
    <name>B5DKI8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 17">Vesicle-fusing ATPase</fullName>
            <ecNumber evidence="4 17">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">comt</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 17">Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="4 17"/>
    <dbReference type="RefSeq" id="XP_002133409.1">
        <property type="nucleotide sequence ID" value="XM_002133373.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKI8"/>
    <dbReference type="SMR" id="B5DKI8"/>
    <dbReference type="FunCoup" id="B5DKI8">
        <property type="interactions" value="1476"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DKI8"/>
    <dbReference type="GeneID" id="6901871"/>
    <dbReference type="KEGG" id="dpo:6901871"/>
    <dbReference type="eggNOG" id="KOG0741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008037_2_0_1"/>
    <dbReference type="InParanoid" id="B5DKI8"/>
    <dbReference type="OMA" id="VINWGTP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244234">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19504">
        <property type="entry name" value="RecA-like_NSF-SEC18_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000010">
        <property type="entry name" value="vesicle-fusing ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000026">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000031">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000166">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000012">
        <property type="entry name" value="Vesicle-fusing ATPase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000187">
        <property type="entry name" value="Vesicular-fusion ATPase SEC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054419">
        <property type="entry name" value="NSF_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039812">
        <property type="entry name" value="Vesicle-fus_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078:SF3">
        <property type="entry name" value="VESICLE-FUSING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078">
        <property type="entry name" value="VESICULAR-FUSION PROTEIN NSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21964">
        <property type="entry name" value="NSF_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 17" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="19">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="252"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="535"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU367045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_002133409.1"/>
        </source>
    </evidence>
    <sequence length="745" mass="82606" checksum="59D0DF6EF501C77B" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>B5DL64</accession>
    <accession>A0A6I8UYT7</accession>
    <name>B5DL64_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chymotrypsin-elastase inhibitor ixodidin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133636.2">
        <property type="nucleotide sequence ID" value="XM_002133600.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DL64"/>
    <dbReference type="FunCoup" id="B5DL64">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DL64"/>
    <dbReference type="GeneID" id="6902105"/>
    <dbReference type="KEGG" id="dpo:6902105"/>
    <dbReference type="eggNOG" id="ENOG502SX7T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156801_0_1_1"/>
    <dbReference type="InParanoid" id="B5DL64"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244108">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DL64">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000674">
        <property type="entry name" value="Mucin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133636.2"/>
        </source>
    </evidence>
    <sequence length="92" mass="10035" checksum="1B079AFEE1A4F6F4" modified="2016-01-20" version="2">MMSVSWNQLCLSLLLIVCSLLALTTAQPPITPRKCPANESYLPCGPSCQTECSTLGQPCLIRHIRCPDGCYCNEGYARNSRGACIPQGQCRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-01-04" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>P84329</accession>
    <accession>Q29J19</accession>
    <name>HFW1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Protein halfway</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein singed wings</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hfw</name>
        <name type="ORF">GA15922</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="BMC Genet." volume="5" first="15" last="15">
            <title>Molecular characterization of the singed wings locus of Drosophila melanogaster.</title>
            <authorList>
                <person name="Schwartz Y.B."/>
                <person name="Boykova T."/>
                <person name="Belyaeva E.S."/>
                <person name="Ashburner M."/>
                <person name="Zhimulev I.F."/>
            </authorList>
            <dbReference type="PubMed" id="15189568"/>
            <dbReference type="DOI" id="10.1186/1471-2156-5-15"/>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Has a role in the ecdysone induced cascade; probably indirect control of 'late' ecdysone genes.</text>
    </comment>
    <dbReference type="EMBL" id="CH379063">
        <property type="protein sequence ID" value="EAL32483.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P84329"/>
    <dbReference type="SMR" id="P84329"/>
    <dbReference type="FunCoup" id="P84329">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="46245.P84329"/>
    <dbReference type="GlyCosmos" id="P84329">
        <property type="glycosylation" value="2 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028059_2_0_1"/>
    <dbReference type="InParanoid" id="P84329"/>
    <dbReference type="OMA" id="RLQCDCN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035073">
        <property type="term" value="P:pupariation"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035075">
        <property type="term" value="P:response to ecdysone"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000867">
        <property type="entry name" value="Blast:Protein halfway"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001217">
        <property type="entry name" value="Protein halfway"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0433">Leucine-rich repeat</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000083962" description="Protein halfway">
        <location>
            <begin position="1"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT">
        <location>
            <begin position="347"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 1">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 2">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 3">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT">
        <location>
            <begin position="489"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9W568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="595" mass="66567" checksum="55A798752266C7A9" modified="2006-06-13" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DL6</accession>
    <accession>A0A6I8UBK8</accession>
    <name>Q29DL6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein obstructor-E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4811953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352897.3">
        <property type="nucleotide sequence ID" value="XM_001352861.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DL6"/>
    <dbReference type="STRING" id="46245.Q29DL6"/>
    <dbReference type="GeneID" id="4811953"/>
    <dbReference type="KEGG" id="dpo:4811953"/>
    <dbReference type="eggNOG" id="ENOG502TESP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062693_1_0_1"/>
    <dbReference type="InParanoid" id="Q29DL6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070582">
        <property type="expression patterns" value="Expressed in multicellular organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DL6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="97"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="136"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="215"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352897.3"/>
        </source>
    </evidence>
    <sequence length="269" mass="29909" checksum="651806FEE5AE7049" modified="2016-01-20" version="3">MMYRLTLLALTALIGSYGAMGADINICSGVVSNLFLPHISNCSQYYLCMSETAVPRECPQGYYFDATDQQCVVVEEVRCLPTCPAKGLTSFCYDRTCTKYVLCFDGTPVLRQCSDGLQYNAQTDRCDYPQYVDCVDNLCVRQNNPAAIVYIASKSLCDKYFVCVDGLPQVRNCTRGLQYNAATTSCDFASKVNCTVETLQRNILPYAKAPPRSAGIVCPAEGTHFYAHKNRQDSYYYCLNGRGVTLDCTPGLVYDAKREECREPQFATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E60</accession>
    <accession>A0A6I8UB47</accession>
    <name>Q29E60_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4811951</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ribosomal large subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RRS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352703.2">
        <property type="nucleotide sequence ID" value="XM_001352667.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E60"/>
    <dbReference type="FunCoup" id="Q29E60">
        <property type="interactions" value="1441"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E60"/>
    <dbReference type="GeneID" id="4811951"/>
    <dbReference type="KEGG" id="dpo:4811951"/>
    <dbReference type="eggNOG" id="KOG1765">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065163_1_0_1"/>
    <dbReference type="InParanoid" id="Q29E60"/>
    <dbReference type="OMA" id="ACDKNRI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076895">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29E60">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007023">
        <property type="entry name" value="Ribosom_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17602">
        <property type="entry name" value="RIBOSOME BIOGENESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17602:SF4">
        <property type="entry name" value="RIBOSOME BIOGENESIS REGULATORY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04939">
        <property type="entry name" value="RRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="286"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352703.2"/>
        </source>
    </evidence>
    <sequence length="344" mass="38976" checksum="01DC35DB4BCDBF34" modified="2008-10-14" version="2">MDVVKEVLAKHQKELEKYKPITVDKHLECRLDVGTLLITDPNDFDDRELRDNREEYLTALTRDNTQLLLNAIWELPTERVDESIVAKLPEPTTVLPRLRKVPGPRPLTKWEQFAKEKGITKKKRDKKTYDDTLDKWVPTYGFKHAEAEKSKDWVLEVPQNADPNTDMFQKKIDLRNEKVAKNEIQRMKNIVRARKVDMPRSGYLGPEAASSSQLLTAVTVAKSSTASVGKFQNKLPKEKEARGLGVKELIPGGKRKASHITNNEREANLDLIKSVLHKKPKLNVDKAISMQKEEDRLAREAEGAPARKAKPKGKGSRKAVGKKPKGNHGQRAPGKKAQAGRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q29F28</accession>
    <accession>A0A6I8UAE6</accession>
    <name>Q29F28_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphatidate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4812264</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352385.1">
        <property type="nucleotide sequence ID" value="XM_001352349.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29F28"/>
    <dbReference type="FunCoup" id="Q29F28">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29F28"/>
    <dbReference type="GeneID" id="4812264"/>
    <dbReference type="KEGG" id="dpo:4812264"/>
    <dbReference type="eggNOG" id="KOG3030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021458_3_2_1"/>
    <dbReference type="InParanoid" id="Q29F28"/>
    <dbReference type="OMA" id="FKPCTRP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071061">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29F28">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF197">
        <property type="entry name" value="FI04477P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="108"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001352385.1"/>
        </source>
    </evidence>
    <sequence length="343" mass="37932" checksum="DBC353D65CFD298B" modified="2006-04-04" version="1">MSKYTKLARGICDLLIWAALSVGSLLLHKWGKPFKRGFFCGDESLAYPYRESSFEHELVLAIAFGVPTSVIVVVELFKQLPGGALRETSGKRDGCRLGHRLGQLYKQAIFYLFGLAMVAFTTMLSKVCIGRLRPHFFAVCQPMLPDGSTCQDAQNQGRYIDSYTCSNANMTDFNFRQLNQSFPSGHASIILYAMLYLAIYLQAALSTRVSKLLKHLLQFLFVMFGWYVSLTRITDYHHHWSDVLAGAVMGVLIACLTGAYVADLFAFKRWPKTGYSANTLRKPQVSPKSSTKSQAGAAPATAASNGAQPPALPAYTFGTLPYLAAHPAQAQYAQTYHNYGYVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q29F37</accession>
    <accession>A0A6I8UEE6</accession>
    <name>Q29F37_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="3 8">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">sgl</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="3 8"/>
    <dbReference type="RefSeq" id="XP_001354183.1">
        <property type="nucleotide sequence ID" value="XM_001354147.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29F37"/>
    <dbReference type="SMR" id="Q29F37"/>
    <dbReference type="FunCoup" id="Q29F37">
        <property type="interactions" value="1331"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29F37"/>
    <dbReference type="GeneID" id="4814032"/>
    <dbReference type="KEGG" id="dpo:4814032"/>
    <dbReference type="eggNOG" id="KOG2666">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_7_0_1"/>
    <dbReference type="InParanoid" id="Q29F37"/>
    <dbReference type="OMA" id="CFIAVGT"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070109">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29F37">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000001">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000032">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000114">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028356">
        <property type="entry name" value="UDPglc_DH_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374:SF3">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374">
        <property type="entry name" value="UDP-GLUCOSE DEHYDROGENASE/UDP-MANNAC DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500133">
        <property type="entry name" value="UDPglc_DH_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="328"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="85"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="126"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="272"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500133-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500133-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001354183.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="52893" checksum="50DF45660E2B3F0D" modified="2006-04-04" version="1">MKVCCIGAGYVGGPTCAVMALKCPDIVITLVDKSVERIAQWNSDKLPIYEPGLDEVVKKCRNVNLFFSTDIETAIKEADLIFISVNTPTKVSGNGKGRAADLKYVESAARMIAEIAQSNKIVVEKSTVPVRAAESIMHILRANQKPGIHYDILSNPEFLAEGTAINDLLNADRVLIGGEETTEGHEAVAKLSWIYEHWIPKQHILTTNTWSSELSKLAANAFLAQRISSINSLSAVCEATGADVSEVARAVGLDSRIGSKFLQASVGFGGSCFQKDILNLIYICENLNLPEVATYWQQVIDMNDYQKRRFSQKIIESLFNTVSDKRIAILGFAFKKNTGDTRETAAITVCQTLLEEGAKLDIYDPKVEPEQIIDDLTHPSVTESPENVKKAVQIHSDPYSAVRATHALVICTEWDEFVDLDFQRIYQSMMKPAYIFDGRKILDHERLHQIGFHVQTIGKKYQRSGLLRSWGIVPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FL0</accession>
    <accession>A0A6I8UEN5</accession>
    <name>Q29FL0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GATOR complex protein NPRL2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Nprl2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPR2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354337.1">
        <property type="nucleotide sequence ID" value="XM_001354301.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FL0"/>
    <dbReference type="FunCoup" id="Q29FL0">
        <property type="interactions" value="781"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FL0"/>
    <dbReference type="GeneID" id="4814194"/>
    <dbReference type="KEGG" id="dpo:4814194"/>
    <dbReference type="eggNOG" id="KOG3789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014995_0_0_1"/>
    <dbReference type="InParanoid" id="Q29FL0"/>
    <dbReference type="OMA" id="IVMHKPD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081531">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009348">
        <property type="entry name" value="NPR2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991:SF10">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991">
        <property type="entry name" value="NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06218">
        <property type="entry name" value="NPR2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354337.1"/>
        </source>
    </evidence>
    <sequence length="393" mass="44797" checksum="3D59D3D2E3B2D4D8" modified="2006-04-04" version="1">MHSHTNDAAGTSSAGSTEGKIRCIFLSEFHATAGCKISCQVPDNYISKDVFDAINVYIIPKQHLQRCILTVNAMDVKIVGYPVGIQDQQKYARNAFLFNLCFVCDSRARSVQYEPVVKKLSEYLIMMEEESCFLSREDDKRRLQNIFQTVLRDLNERKVATIVEGDNTIYLKIVMHKPDPPAVKDHMVPLLLANLRDTPLENWDLTTQQILPYINGINHVARIAAEADVETDLVKSCIQNLVYYGVVQLLPILKYSNVYMTQNLKHLIQSASLSNACRKYVALKPDKTLPSVQRIFQFYASMTHGVTLRAICQRLCPQHHNIDERKMVIFGLQHKFIRCIHKYPVFTGSVPSGRQKMYTGLISFDEICCKTGLSPCTIERDIEKDTNVTVIWK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-12-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29I35</accession>
    <accession>B5DLU2</accession>
    <name>ARM_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Armadillo segment polarity protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">arm</name>
        <name type="ORF">GA27602</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May associate with CadN and participate in the transmission of developmental information. Can associate with alpha-catenin. Accumulates through wg signaling; arm function in wg signal transduction is required early in development for determination of neuroblast fate. Arm and Abl proteins function cooperatively at adherens junctions in both the CNS and epidermis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Adherens junction</location>
        </subcellularLocation>
        <text evidence="1">Inner surface of cell membrane and adherens junction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EDY72488" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EDY72488.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29I35"/>
    <dbReference type="SMR" id="Q29I35"/>
    <dbReference type="STRING" id="46245.Q29I35"/>
    <dbReference type="eggNOG" id="KOG4203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="Q29I35"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014017">
        <property type="term" value="P:neuroblast fate commitment"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007367">
        <property type="term" value="P:segment polarity determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21726">
        <property type="entry name" value="CTNNAbd_dArm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000015">
        <property type="entry name" value="Catenin beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013284">
        <property type="entry name" value="Beta-catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45976">
        <property type="entry name" value="ARMADILLO SEGMENT POLARITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01869">
        <property type="entry name" value="BCATNINFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0130">Cell adhesion</keyword>
    <keyword id="KW-0965">Cell junction</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0709">Segmentation polarity protein</keyword>
    <keyword id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="chain" id="PRO_0000312834" description="Armadillo segment polarity protein">
        <location>
            <begin position="1"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 1">
        <location>
            <begin position="149"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 2">
        <location>
            <begin position="191"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 3">
        <location>
            <begin position="233"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 4">
        <location>
            <begin position="275"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 5">
        <location>
            <begin position="359"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 6">
        <location>
            <begin position="398"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 7">
        <location>
            <begin position="440"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 8">
        <location>
            <begin position="487"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 9">
        <location>
            <begin position="597"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM 10">
        <location>
            <begin position="638"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="765"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="771"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P18824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="832" mass="90293" checksum="52205E16C85BE598" modified="2009-01-20" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JF0</accession>
    <accession>A0A6I8UGV8</accession>
    <name>Q29JF0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein DIP1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">DIP1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355294.2">
        <property type="nucleotide sequence ID" value="XM_001355258.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JF0"/>
    <dbReference type="FunCoup" id="Q29JF0">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JF0"/>
    <dbReference type="GeneID" id="4815697"/>
    <dbReference type="KEGG" id="dpo:4815697"/>
    <dbReference type="eggNOG" id="ENOG502S3FH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039772_0_0_1"/>
    <dbReference type="InParanoid" id="Q29JF0"/>
    <dbReference type="OMA" id="EWERDGY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074639">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JF0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070578">
        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070920">
        <property type="term" value="P:regulation of regulatory ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00048">
        <property type="entry name" value="DSRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051247">
        <property type="entry name" value="RLC_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205:SF5">
        <property type="entry name" value="BLANKS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="215"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355294.2"/>
        </source>
    </evidence>
    <sequence length="392" mass="42705" checksum="C0C75A78BD4C0E0F" modified="2008-10-14" version="2">MKRSRRATSANSKAFVSGGVYNPNVPAKPLIQPPANALSQASPVSAPTPTPVPAPVAVKEVQAKKKDAPPSVSALPIPIFVKDEPVAMAEDESSPIESASEGFYIDISADNGNGGKIPFKKIFQKRKKSSERTRDKKQRQNRQLRKSMLPKNALMALNELKGVNISDFTINSNPDGGFTAILTVNSNQYHGKGVSKMAAKNLACEHALRDYIIAKMKPKPRKPKNNAVENQASTGPEPMDTNGSDTESPEDELPMLNLASFAIYKLFSEWERDGYVVPEMHPSANAQPQQGDANAAVPVVPKEPKKPPVRAELPPNWQTMHPATLLCIMRPGIAYEDYGTAGDKPNVPQCLGVIVDNQEFTANGRSKKIARRNVAVNVCNTLFGTNFTYEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LI3</accession>
    <accession>A0A6I8UKK9</accession>
    <name>Q29LI3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine protease</fullName>
            <ecNumber evidence="11">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Atg4a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001356996.1">
        <property type="nucleotide sequence ID" value="XM_001356960.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LI3"/>
    <dbReference type="SMR" id="Q29LI3"/>
    <dbReference type="FunCoup" id="Q29LI3">
        <property type="interactions" value="671"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LI3"/>
    <dbReference type="MEROPS" id="C54.A04"/>
    <dbReference type="GeneID" id="4817593"/>
    <dbReference type="KEGG" id="dpo:4817593"/>
    <dbReference type="eggNOG" id="KOG2674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021259_0_0_1"/>
    <dbReference type="InParanoid" id="Q29LI3"/>
    <dbReference type="OMA" id="TGFGCMI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078184">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LI3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF49">
        <property type="entry name" value="CYSTEINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11 15" id="KW-0645">Protease</keyword>
    <keyword evidence="8 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="13">
        <location>
            <begin position="65"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001356996.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="45720" checksum="5AC4BC55E574620B" modified="2006-04-04" version="1">MLVILADQLTRIMESVFEAYLGPDSVLASAVGQAVGSGEPEDIPKRNTDVWVLGRRYNAIQELEVIRRDIQSRLWCTYRHGFMPLGEVQLTTDRGWGCMLRCGQMVLAQALIDLHLGRDWFWTPECQDATYLKIVNRFEDVRKSYYSIHQIALMGESQNKAVGEWLGPNTVAQILKKLVLFDDWCSLVVHVAMDSTVVLDDVYSLCLEGDAWKPLLLIIPLRLGISDINPIYIPALKRCLELDSSCGMIGGRPNQALYFLGYVEDEVLYLDPHTTQRTGVVGQKTSSGEQEHDETYHQKHAARLSFSAMDPSLAVCFLCKTSDSFQQLLEKLRQEVLGMCSPALFEISQTRAVDWDTAEDIEWPTMPDIDWPAGTSDSDSFAIVEESVRDGDAAGTSSSGSKPSERAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LW2</accession>
    <accession>A0A6I8UKK7</accession>
    <name>Q29LW2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine/threonine-protein kinase TBK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IKKepsilon</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356867.2">
        <property type="nucleotide sequence ID" value="XM_001356831.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LW2"/>
    <dbReference type="SMR" id="Q29LW2"/>
    <dbReference type="FunCoup" id="Q29LW2">
        <property type="interactions" value="1533"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LW2"/>
    <dbReference type="GeneID" id="4816995"/>
    <dbReference type="KEGG" id="dpo:4816995"/>
    <dbReference type="eggNOG" id="KOG4250">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_101_1_1"/>
    <dbReference type="InParanoid" id="Q29LW2"/>
    <dbReference type="OMA" id="HECKRRI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075421">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LW2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045089">
        <property type="term" value="P:positive regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12219">
        <property type="entry name" value="Ubl_TBK1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000267">
        <property type="entry name" value="Blast:Serine/threonine-protein kinase TBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.420:FF:000003">
        <property type="entry name" value="IKK epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000100">
        <property type="entry name" value="inhibitor of nuclear factor kappa-B kinase subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000106">
        <property type="entry name" value="serine/threonine-protein kinase TBK1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051180">
        <property type="entry name" value="IKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041309">
        <property type="entry name" value="TBK1_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041087">
        <property type="entry name" value="TBK1_ULD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969:SF15">
        <property type="entry name" value="FI05319P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969">
        <property type="entry name" value="IKB KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18394">
        <property type="entry name" value="TBK1_CCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18396">
        <property type="entry name" value="TBK1_ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356867.2"/>
        </source>
    </evidence>
    <sequence length="719" mass="82160" checksum="1B10C56F3B52115A" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZE0</accession>
    <accession>A0A6I8UDS6</accession>
    <name>Q2LZE0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endocuticle structural protein SgAbd-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Cpr65Av</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353833.1">
        <property type="nucleotide sequence ID" value="XM_001353797.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZE0"/>
    <dbReference type="FunCoup" id="Q2LZE0">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZE0"/>
    <dbReference type="GeneID" id="5495511"/>
    <dbReference type="KEGG" id="dpo:5495511"/>
    <dbReference type="eggNOG" id="ENOG502ST8A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_7_3_1"/>
    <dbReference type="InParanoid" id="Q2LZE0"/>
    <dbReference type="OMA" id="MHSINAC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076891">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZE0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF218">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 65AA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
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        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353833.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12002" checksum="C08193ABF7EB95F0" modified="2006-02-21" version="1">MQSIKVCVLALCAFVLAASVSAAPLDDSKQATILRYDNDNIGTDGYNFGYETSDGVTRQEQAELKNAGTDHEALSVRGSVSWVAPDGQTYTLHYIADENGFQPQGDHLPHN</sequence>
</entry>
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    <accession>Q2M152</accession>
    <accession>A0A6I8UCA1</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="primary">LOC4813680</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <strain evidence="2">MV-25-SWS-2005</strain>
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    </reference>
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        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353221.1"/>
        </source>
    </evidence>
    <sequence length="67" mass="7326" checksum="FF160EA609DAAEF7" modified="2006-02-21" version="1">MLDPISDTPLVLAYAFMSLLVLILCFILVNVVHKLYRSINSGDPISSPISTHELKTSIAEIDPLKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NX07</accession>
    <accession>A0A6I8VY92</accession>
    <name>A0A0R3NX07_DROPS</name>
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        <submittedName>
            <fullName evidence="6 7 8">Inactive dipeptidyl peptidase 10</fullName>
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    <gene>
        <name evidence="6 7 8" type="primary">LOC6903318</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7 8">MV-25-SWS-2005</strain>
            <tissue evidence="6 7 8">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033379608.1"/>
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        <property type="nucleotide sequence ID" value="XM_033379609.1"/>
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        <property type="nucleotide sequence ID" value="XM_033379610.1"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:dipeptidyl-peptidase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
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        <property type="entry name" value="Peptidase_S9_cat"/>
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    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
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    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF187">
        <property type="entry name" value="INACTIVE DIPEPTIDYL PEPTIDASE 10-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="4">
        <location>
            <begin position="143"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="3">
        <location>
            <begin position="638"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235499.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033235500.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033235501.1"/>
        </source>
    </evidence>
    <sequence length="853" mass="96915" checksum="DE265F151DB77C6C" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NX16</accession>
    <accession>A0A6I8VML9</accession>
    <name>A0A0R3NX16_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein, isoform B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Dpse\GA29172</name>
        <name evidence="4" type="synonym">LOC6903893</name>
        <name evidence="2" type="ORF">Dpse_GA29172</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
                <person name="Castrezana S."/>
                <person name="Celniker S.E."/>
                <person name="Chang J.L."/>
                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
                <person name="Clifton S.W."/>
                <person name="Comeron J.M."/>
                <person name="Costello J.C."/>
                <person name="Coyne J.A."/>
                <person name="Daub J."/>
                <person name="David R.G."/>
                <person name="Delcher A.L."/>
                <person name="Delehaunty K."/>
                <person name="Do C.B."/>
                <person name="Ebling H."/>
                <person name="Edwards K."/>
                <person name="Eickbush T."/>
                <person name="Evans J.D."/>
                <person name="Filipski A."/>
                <person name="Findeiss S."/>
                <person name="Freyhult E."/>
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                <person name="Gardiner A."/>
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                <person name="Gilbert D."/>
                <person name="Gnerre S."/>
                <person name="Godfrey J."/>
                <person name="Good R."/>
                <person name="Gotea V."/>
                <person name="Gravely B."/>
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                <person name="Griffiths-Jones S."/>
                <person name="Gross S."/>
                <person name="Guigo R."/>
                <person name="Gustafson E.A."/>
                <person name="Haerty W."/>
                <person name="Hahn M.W."/>
                <person name="Halligan D.L."/>
                <person name="Halpern A.L."/>
                <person name="Halter G.M."/>
                <person name="Han M.V."/>
                <person name="Heger A."/>
                <person name="Hillier L."/>
                <person name="Hinrichs A.S."/>
                <person name="Holmes I."/>
                <person name="Hoskins R.A."/>
                <person name="Hubisz M.J."/>
                <person name="Hultmark D."/>
                <person name="Huntley M.A."/>
                <person name="Jaffe D.B."/>
                <person name="Jagadeeshan S."/>
                <person name="Jeck W.R."/>
                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
                <person name="Li R."/>
                <person name="Lin C.F."/>
                <person name="Lin M.F."/>
                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
                <person name="Machado C.A."/>
                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
                <person name="O'Grady P."/>
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                <person name="Papaceit M."/>
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                <person name="Parisi M."/>
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                <person name="Puig M."/>
                <person name="Quesneville H."/>
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                <person name="Rand D."/>
                <person name="Rasmussen M.D."/>
                <person name="Reed L.K."/>
                <person name="Reenan R."/>
                <person name="Reily A."/>
                <person name="Remington K.A."/>
                <person name="Rieger T.T."/>
                <person name="Ritchie M.G."/>
                <person name="Robin C."/>
                <person name="Rogers Y.H."/>
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                <person name="Ruiz A."/>
                <person name="Russo S."/>
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                <person name="Saranga D.J."/>
                <person name="Sato H."/>
                <person name="Schaeffer S.W."/>
                <person name="Schatz M.C."/>
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                <person name="Schwartz R."/>
                <person name="Segarra C."/>
                <person name="Singh R.S."/>
                <person name="Sirot L."/>
                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
                <person name="Smith T.F."/>
                <person name="Spieth J."/>
                <person name="Stage D.E."/>
                <person name="Stark A."/>
                <person name="Stephan W."/>
                <person name="Strausberg R.L."/>
                <person name="Strempel S."/>
                <person name="Sturgill D."/>
                <person name="Sutton G."/>
                <person name="Sutton G.G."/>
                <person name="Tao W."/>
                <person name="Teichmann S."/>
                <person name="Tobari Y.N."/>
                <person name="Tomimura Y."/>
                <person name="Tsolas J.M."/>
                <person name="Valente V.L."/>
                <person name="Venter E."/>
                <person name="Venter J.C."/>
                <person name="Vicario S."/>
                <person name="Vieira F.G."/>
                <person name="Vilella A.J."/>
                <person name="Villasante A."/>
                <person name="Walenz B."/>
                <person name="Wang J."/>
                <person name="Wasserman M."/>
                <person name="Watts T."/>
                <person name="Wilson D."/>
                <person name="Wilson R.K."/>
                <person name="Wing R.A."/>
                <person name="Wolfner M.F."/>
                <person name="Wong A."/>
                <person name="Wong G.K."/>
                <person name="Wu C.I."/>
                <person name="Wu G."/>
                <person name="Yamamoto D."/>
                <person name="Yang H.P."/>
                <person name="Yang S.P."/>
                <person name="Yorke J.A."/>
                <person name="Yoshida K."/>
                <person name="Zdobnov E."/>
                <person name="Zhang P."/>
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                <person name="Zimin A.V."/>
                <person name="Baldwin J."/>
                <person name="Abdouelleil A."/>
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                <person name="Abebe A."/>
                <person name="Abera B."/>
                <person name="Abreu J."/>
                <person name="Acer S.C."/>
                <person name="Aftuck L."/>
                <person name="Alexander A."/>
                <person name="An P."/>
                <person name="Anderson E."/>
                <person name="Anderson S."/>
                <person name="Arachi H."/>
                <person name="Azer M."/>
                <person name="Bachantsang P."/>
                <person name="Barry A."/>
                <person name="Bayul T."/>
                <person name="Berlin A."/>
                <person name="Bessette D."/>
                <person name="Bloom T."/>
                <person name="Blye J."/>
                <person name="Boguslavskiy L."/>
                <person name="Bonnet C."/>
                <person name="Boukhgalter B."/>
                <person name="Bourzgui I."/>
                <person name="Brown A."/>
                <person name="Cahill P."/>
                <person name="Channer S."/>
                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
                <person name="Costello M."/>
                <person name="D'Aco K."/>
                <person name="Daza R."/>
                <person name="De Haan G."/>
                <person name="DeGray S."/>
                <person name="DeMaso C."/>
                <person name="Dhargay N."/>
                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
                <person name="Dorjee K."/>
                <person name="Dupes A."/>
                <person name="Elong R."/>
                <person name="Falk J."/>
                <person name="Farina A."/>
                <person name="Faro S."/>
                <person name="Ferguson D."/>
                <person name="Fisher S."/>
                <person name="Foley C.D."/>
                <person name="Franke A."/>
                <person name="Friedrich D."/>
                <person name="Gadbois L."/>
                <person name="Gearin G."/>
                <person name="Gearin C.R."/>
                <person name="Giannoukos G."/>
                <person name="Goode T."/>
                <person name="Graham J."/>
                <person name="Grandbois E."/>
                <person name="Grewal S."/>
                <person name="Gyaltsen K."/>
                <person name="Hafez N."/>
                <person name="Hagos B."/>
                <person name="Hall J."/>
                <person name="Henson C."/>
                <person name="Hollinger A."/>
                <person name="Honan T."/>
                <person name="Huard M.D."/>
                <person name="Hughes L."/>
                <person name="Hurhula B."/>
                <person name="Husby M.E."/>
                <person name="Kamat A."/>
                <person name="Kanga B."/>
                <person name="Kashin S."/>
                <person name="Khazanovich D."/>
                <person name="Kisner P."/>
                <person name="Lance K."/>
                <person name="Lara M."/>
                <person name="Lee W."/>
                <person name="Lennon N."/>
                <person name="Letendre F."/>
                <person name="LeVine R."/>
                <person name="Lipovsky A."/>
                <person name="Liu X."/>
                <person name="Liu J."/>
                <person name="Liu S."/>
                <person name="Lokyitsang T."/>
                <person name="Lokyitsang Y."/>
                <person name="Lubonja R."/>
                <person name="Lui A."/>
                <person name="MacDonald P."/>
                <person name="Magnisalis V."/>
                <person name="Maru K."/>
                <person name="Matthews C."/>
                <person name="McCusker W."/>
                <person name="McDonough S."/>
                <person name="Mehta T."/>
                <person name="Meldrim J."/>
                <person name="Meneus L."/>
                <person name="Mihai O."/>
                <person name="Mihalev A."/>
                <person name="Mihova T."/>
                <person name="Mittelman R."/>
                <person name="Mlenga V."/>
                <person name="Montmayeur A."/>
                <person name="Mulrain L."/>
                <person name="Navidi A."/>
                <person name="Naylor J."/>
                <person name="Negash T."/>
                <person name="Nguyen T."/>
                <person name="Nguyen N."/>
                <person name="Nicol R."/>
                <person name="Norbu C."/>
                <person name="Norbu N."/>
                <person name="Novod N."/>
                <person name="O'Neill B."/>
                <person name="Osman S."/>
                <person name="Markiewicz E."/>
                <person name="Oyono O.L."/>
                <person name="Patti C."/>
                <person name="Phunkhang P."/>
                <person name="Pierre F."/>
                <person name="Priest M."/>
                <person name="Raghuraman S."/>
                <person name="Rege F."/>
                <person name="Reyes R."/>
                <person name="Rise C."/>
                <person name="Rogov P."/>
                <person name="Ross K."/>
                <person name="Ryan E."/>
                <person name="Settipalli S."/>
                <person name="Shea T."/>
                <person name="Sherpa N."/>
                <person name="Shi L."/>
                <person name="Shih D."/>
                <person name="Sparrow T."/>
                <person name="Spaulding J."/>
                <person name="Stalker J."/>
                <person name="Stange-Thomann N."/>
                <person name="Stavropoulos S."/>
                <person name="Stone C."/>
                <person name="Strader C."/>
                <person name="Tesfaye S."/>
                <person name="Thomson T."/>
                <person name="Thoulutsang Y."/>
                <person name="Thoulutsang D."/>
                <person name="Topham K."/>
                <person name="Topping I."/>
                <person name="Tsamla T."/>
                <person name="Vassiliev H."/>
                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
                <person name="Weiand M."/>
                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
                <person name="Yu Q."/>
                <person name="Zembek L."/>
                <person name="Zhong D."/>
                <person name="Zimmer A."/>
                <person name="Zwirko Z."/>
                <person name="Jaffe D.B."/>
                <person name="Alvarez P."/>
                <person name="Brockman W."/>
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                <person name="Chin C."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
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                <person name="Mauceli E."/>
                <person name="MacCallum I."/>
            </authorList>
            <dbReference type="PubMed" id="17994087"/>
            <dbReference type="DOI" id="10.1038/nature06341"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
            <authorList>
                <person name="English A.C."/>
                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CH674332">
        <property type="protein sequence ID" value="KRT05646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044581.1">
        <property type="nucleotide sequence ID" value="XM_015189095.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NX16"/>
    <dbReference type="GeneID" id="6903893"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250530">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NX16">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KRT05646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015044581.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="24651" checksum="FA8BF44CF51FA7D8" modified="2016-01-20" version="1">MSDTKKIGDRPSRRRDHERDPEWNQEQNNIMFNRLLQTLATEQQKLMVLEESRGRLMEDMRNLRARFMQENALLRRIIQRGASLEDVESLRANPSRSATRSMSFARQSPASSRQRKPTVPQQVTSRVSLKKEEQRTSRVSLKKEEQRQDAPANDPAGSRSVQVPKRVPGPNSSPKPNPIRKFRKQRQRNIHVSAQNRQKIEASQEHDPDDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P547</accession>
    <accession>A0A6I8VKM6</accession>
    <name>A0A0R3P547_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">palmitoyl-protein hydrolase</fullName>
            <ecNumber evidence="3">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 14 15 16" type="primary">Apt1</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14 15">MV-25-SWS-2005</strain>
            <tissue evidence="13 14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="3"/>
    <dbReference type="RefSeq" id="XP_015043298.1">
        <property type="nucleotide sequence ID" value="XM_015187812.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043299.1">
        <property type="nucleotide sequence ID" value="XM_015187813.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043301.1">
        <property type="nucleotide sequence ID" value="XM_015187815.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043303.1">
        <property type="nucleotide sequence ID" value="XM_015187817.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P547"/>
    <dbReference type="SMR" id="A0A0R3P547"/>
    <dbReference type="STRING" id="46245.A0A0R3P547"/>
    <dbReference type="GeneID" id="4812911"/>
    <dbReference type="eggNOG" id="KOG2112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075118">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000010">
        <property type="entry name" value="Acyl-protein thioesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF68">
        <property type="entry name" value="PALMITOYL-PROTEIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="11">
        <location>
            <begin position="3"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015043298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015043299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015043301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015043303.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="25053" checksum="F6827596A5DD835F" modified="2016-01-20" version="1">MSAPVIVEATAKQTATLIFMHGLGDTGHGWSSALAAIRPPFMKVICPTAPTQPVSLNAGFRMPSWFDLKTLDISGPEDEPGIQAARDNVHGMIQKEISAGIPANRIVLGGFSQGGALALYSALTYDQPLAGVVALSCWLPLHKKFPGAKLNSEDVPIFQAHGDYDPVVPYKFGQLSASLLKSFMKNVTFKTYSGLSHSSSDDEMDDVKDIISKWTHWEGQSMARKAQCCQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBK3</accession>
    <name>A0A6I8UBK3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="12">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Ddx1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity.</text>
    </comment>
    <comment type="function">
        <text evidence="12">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="12">The helicase domain is involved in the stimulation of RELA transcriptional activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DDX1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="12"/>
    <dbReference type="RefSeq" id="XP_001352892.3">
        <property type="nucleotide sequence ID" value="XM_001352856.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBK3"/>
    <dbReference type="FunCoup" id="A0A6I8UBK3">
        <property type="interactions" value="2418"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812030"/>
    <dbReference type="InParanoid" id="A0A6I8UBK3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17938">
        <property type="entry name" value="DEADc_DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12873">
        <property type="entry name" value="SPRY_DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000076">
        <property type="entry name" value="ATP-dependent RNA helicase DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000652">
        <property type="entry name" value="ATP-dependent RNA helicase DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000708">
        <property type="entry name" value="ATP-dependent RNA helicase DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000716">
        <property type="entry name" value="ATP-dependent RNA helicase DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 12 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="13">
        <location>
            <begin position="70"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="246"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="484"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001352892.3"/>
        </source>
    </evidence>
    <sequence length="728" mass="81068" checksum="363FA89B6D782752" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE56</accession>
    <name>A0A6I8UE56_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354031.2">
        <property type="nucleotide sequence ID" value="XM_001353995.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE56"/>
    <dbReference type="KEGG" id="dpo:4813834"/>
    <dbReference type="InParanoid" id="A0A6I8UE56"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354031.2"/>
        </source>
    </evidence>
    <sequence length="121" mass="14156" checksum="4AC6E9AD4F5701CD" modified="2020-08-12" version="1">MLFLAPRKHLFATNLIILLMIYVMRKCLQLFCILENRTACPQPDCDCDERDREEEKEKEEDSPLMERRGGFKFIPDAMHKSMHGFGYGEGHYQIFVEKKERNLPTHSRLNAATAEVKLNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG74</accession>
    <name>A0A6I8UG74_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="3">4.2.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Hydroxymethylbilane hydrolyase [cyclizing]</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Uroporphyrinogen-III cosynthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Uros1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>hydroxymethylbilane = uroporphyrinogen III + H2O</text>
            <dbReference type="Rhea" id="RHEA:18965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="EC" id="4.2.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uroporphyrinogen-III synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.75" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001354986.3">
        <property type="nucleotide sequence ID" value="XM_001354950.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UG74"/>
    <dbReference type="FunCoup" id="A0A6I8UG74">
        <property type="interactions" value="477"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815378"/>
    <dbReference type="InParanoid" id="A0A6I8UG74"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006785">
        <property type="term" value="P:heme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
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    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10090:FF:000003">
        <property type="entry name" value="uroporphyrinogen-III synthase"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
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    <dbReference type="PANTHER" id="PTHR12390">
        <property type="entry name" value="UROPORPHYRINOGEN III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390:SF0">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
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    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="12">
        <location>
            <begin position="19"/>
            <end position="250"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004772"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008133"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001354986.3"/>
        </source>
    </evidence>
    <sequence length="287" mass="31669" checksum="DF10FD4489109A58" modified="2020-08-12" version="1">MTSRQRTVIIFKSESDSTDVYAETLLGHNFNPVFVPTLCFGFKNLQELGNKLQTPDKYAGIIFTSPRCVEAVNEALLLGELPGGWKLLHNYAVGEVTHNLALSTLQQLFTHGKQTGNARNLGEYIVDTFDGSRDLPLLLPCGNLATDTLLSKLAENGFCVDACEVYETKCSPQLGECVERALKEAGDSVEFLAFFSPSGVACAMEYFRAHNISLEPWKLVAIGPSTRRALESQGQKVYCTAERPTVEHLVKVLLNPQDSRERLVREREQMQELERAAAAAAAALENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGF5</accession>
    <name>A0A6I8UGF5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4814553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355108.2">
        <property type="nucleotide sequence ID" value="XM_001355072.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGF5"/>
    <dbReference type="FunCoup" id="A0A6I8UGF5">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814553"/>
    <dbReference type="InParanoid" id="A0A6I8UGF5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGF5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355108.2"/>
        </source>
    </evidence>
    <sequence length="632" mass="69752" checksum="8C5BF9333FC0FA5F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGG3</accession>
    <name>A0A6I8UGG3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rapamycin-insensitive companion of mTOR isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rictor</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RICTOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355013.2">
        <property type="nucleotide sequence ID" value="XM_001354977.3"/>
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    <dbReference type="FunCoup" id="A0A6I8UGG3">
        <property type="interactions" value="524"/>
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    <dbReference type="KEGG" id="dpo:4814634"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028268">
        <property type="entry name" value="Pianissimo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028267">
        <property type="entry name" value="Pianissimo_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029453">
        <property type="entry name" value="Rictor_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029451">
        <property type="entry name" value="RICTOR_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029452">
        <property type="entry name" value="RICTOR_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298">
        <property type="entry name" value="CYTOSOLIC REGULATOR PIANISSIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298:SF11">
        <property type="entry name" value="RAPAMYCIN-INSENSITIVE COMPANION OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14663">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14666">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14664">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14668">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01303">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01307">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01308">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01310">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR N-terminal" evidence="4">
        <location>
            <begin position="60"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR middle" evidence="3">
        <location>
            <begin position="581"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR" evidence="5">
        <location>
            <begin position="978"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1168"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1437"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1698"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1783"/>
            <end position="1817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1836"/>
            <end position="1873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1925"/>
            <end position="1964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1438"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1476"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1702"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1725"/>
            <end position="1753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1806"/>
            <end position="1817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1935"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1951"/>
            <end position="1964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001355013.2"/>
        </source>
    </evidence>
    <sequence length="1964" mass="216029" checksum="8DE19B9E23D2A91E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIF1</accession>
    <name>A0A6I8UIF1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Or23a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355989.2">
        <property type="nucleotide sequence ID" value="XM_001355953.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIF1"/>
    <dbReference type="FunCoup" id="A0A6I8UIF1">
        <property type="interactions" value="24"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:odorant binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="entry name" value="7tm6_olfct_rcpt"/>
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        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="127"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="165"/>
            <end position="184"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="211"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355989.2"/>
        </source>
    </evidence>
    <sequence length="386" mass="43826" checksum="96DF6F29502ACE87" modified="2020-08-12" version="1">MTPKEPPTRSDYFRDQWRAWRVLGALQLNARRYWSGAMLLNIVVALYAPALLLSMFSFETPLENITNFSLSITSTATVLKFGLYVLQLGRVGDLEKVIARLDDRVEGAEQLRCHRQMAKKLRHLSNLFLYTYGFVLLNSEFSFLFRSGRSLPFPSWFPFDWKTSTVNYIGALGFQLVAIFGQILQNFVDDSFPPLALCLCAGHCQLLILRISSIGHAPSEQEANEAELVECIKDQKELYGLLELTRDVISWPMLIQFFVMALNIGATMFGLVFFADTMQDRMYFVSYLLALVLQTYPVCYYGSLLEDGFGHLHYAVFGSNWVSQSRSYRASMTILGERTKRHPRLLAGSLVPIHLSTFQGTCKAAYSFFTLIGNTRERAGPDPSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJ14</accession>
    <name>A0A6I8UJ14_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">dnt</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001356278.3">
        <property type="nucleotide sequence ID" value="XM_001356242.4"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
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    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="tyrosine-protein kinase Drl"/>
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    </dbReference>
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        <property type="entry name" value="Tyrosine-protein kinase RYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2170">
        <property type="entry name" value="Wnt, WIF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003306">
        <property type="entry name" value="WIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038677">
        <property type="entry name" value="WIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF349">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02019">
        <property type="entry name" value="WIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00469">
        <property type="entry name" value="WIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50814">
        <property type="entry name" value="WIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026346848" description="receptor protein-tyrosine kinase" evidence="18">
        <location>
            <begin position="33"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="WIF" evidence="20">
        <location>
            <begin position="36"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="318"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001356278.3"/>
        </source>
    </evidence>
    <sequence length="585" mass="64771" checksum="71A79D5AD3B0D067" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJH3</accession>
    <name>A0A6I8UJH3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC4817012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356492.2">
        <property type="nucleotide sequence ID" value="XM_001356456.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235738.1">
        <property type="nucleotide sequence ID" value="XM_033379847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJH3"/>
    <dbReference type="KEGG" id="dpo:4817012"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJH3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356492.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="41113" checksum="6D7A81764571B1F4" modified="2020-08-12" version="1">MQQPQRFIPSVSDLYGTDEIELLLDEIRELELEPVCCQLDDEQDFEIAGSRAGELSLSLESPLGTCTSWDSHANYKQRSGATPLPWGVALHCDPQHLKTPTGIVRILLVLSSAACLACECSAGTVQVGLFLLPLIGRLRLMVFCALFSLLITCLMLFLDISHIALMFPFNWTKVNTWMYLSIGLIFILSSTLLVHMVLCATEYTWVSKHSRDTLLATGIIGYVCALEAFLLSGIASWPWTQYRQVPDDASELFIEDREMTPMSPIICSTELHATPYRNGNAAVSEDLYNQQQQQSSYNQKPYIPAKRPNELNNQRPALGHTQTARTKPNQRYREGYHYHTSRQSPTFVLGDDQGAGPSTSRSNDNSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK78</accession>
    <name>A0A6I8UK78_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyoxylate reductase/hydroxypyruvate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4817036</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356852.4">
        <property type="nucleotide sequence ID" value="XM_001356816.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK78"/>
    <dbReference type="FunCoup" id="A0A6I8UK78">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817036"/>
    <dbReference type="InParanoid" id="A0A6I8UK78"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK78">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000026">
        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF119">
        <property type="entry name" value="FI03731P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="17"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="123"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001356852.4"/>
        </source>
    </evidence>
    <sequence length="333" mass="36299" checksum="569651E348FD6214" modified="2020-08-12" version="1">MSTMSTVRAATAAAFKVLVSHPNVPAPALELLRSRGAETIICQSVPPCREEILQKVSGVDAIFWAHYQPLNAGILDAAGAQLRCVSTMSSGIDFADVPEFKRRQIPLGHTPGVVKNSVADLAIGLMIAAGRHFHAGRSDIESSQWKTELIDWRMGQEIRDSVIGFFGFGGISQAIAKRLQCWDVAKILYHTRTRKENDCEFKAEHVSFERLLQESDFLVVAAPLTDESRGKFDAKAFGQMKSNAVFVNVARGGLVIQSDLHEALTKGQIFAAGLDVTTPEPLPADDPILKLPNCVILPHLGTQTMKTTIEMSLLAANNILNAIEGRPMIRPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULF7</accession>
    <name>A0A6I8ULF7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">LOW QUALITY PROTEIN: homer protein homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">homer</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Homer family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357405.4">
        <property type="nucleotide sequence ID" value="XM_001357369.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8ULF7">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4818011"/>
    <dbReference type="InParanoid" id="A0A6I8ULF7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035256">
        <property type="term" value="F:G protein-coupled glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007216">
        <property type="term" value="P:G protein-coupled glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01206">
        <property type="entry name" value="EVH1_Homer_Vesl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000014">
        <property type="entry name" value="Homer homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045027">
        <property type="entry name" value="Homer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044100">
        <property type="entry name" value="Homer_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10918">
        <property type="entry name" value="HOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="WH1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="117"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="408"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="248"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="423"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357405.4"/>
        </source>
    </evidence>
    <sequence length="562" mass="59915" checksum="086ED10AB5DD43FF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM95</accession>
    <name>A0A6I8UM95_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">uncharacterized protein LOC4800479</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6" type="primary">LOC4800479</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4 5">MV-25-SWS-2005</strain>
            <strain evidence="2 3">MV2-25</strain>
            <tissue evidence="3 4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357746.1">
        <property type="nucleotide sequence ID" value="XM_001357709.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038604.1">
        <property type="nucleotide sequence ID" value="XM_015183118.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038605.1">
        <property type="nucleotide sequence ID" value="XM_015183119.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038606.1">
        <property type="nucleotide sequence ID" value="XM_015183120.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM95"/>
    <dbReference type="GeneID" id="4800479"/>
    <dbReference type="KEGG" id="dpo:4800479"/>
    <dbReference type="OMA" id="IYHGYRM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075366">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015038604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015038605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015038606.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="28353" checksum="2ED318B8FCC56F6A" modified="2020-08-12" version="1">MSYSAERALSYLYPFTIVSSVICCITSAVAWSHWRYVLNACPDTNCGCVLHSRSTYNSFEGGNIAYCHYATYGLLLPLIFAVVLGIYHGYRICIGKGQRKSGHATIRQRSGDMIVVTTESELTPDGLSPYYWLPATVISLIMAIYQLIYASVFTDGFEVTCKQYRESLLKEIQGVGNIVPVLKSRLSCAAIFDFMDFLVESISYERRRYGRINTAACLYFTLIFAWLAVFSWLSICVINIVHLRRTRAARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UML2</accession>
    <name>A0A6I8UML2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lysyl oxidase homolog 2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Loxl1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357948.3">
        <property type="nucleotide sequence ID" value="XM_001357911.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UML2"/>
    <dbReference type="FunCoup" id="A0A6I8UML2">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800728"/>
    <dbReference type="InParanoid" id="A0A6I8UML2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004720">
        <property type="term" value="F:protein-lysine 6-oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000008">
        <property type="entry name" value="Lysyl oxidase homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050912">
        <property type="entry name" value="LOX-like_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001695">
        <property type="entry name" value="Lysyl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817">
        <property type="entry name" value="LYSYL OXIDASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817:SF4">
        <property type="entry name" value="LYSYL OXIDASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01186">
        <property type="entry name" value="Lysyl_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00074">
        <property type="entry name" value="LYSYLOXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026347958" evidence="3">
        <location>
            <begin position="24"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="38"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001357948.3"/>
        </source>
    </evidence>
    <sequence length="365" mass="41642" checksum="C1DAF713389BECA4" modified="2020-08-12" version="1">MESQRLLLLLPLLLVLGLSCCRANSRLNVQNNHRNMMVRLATNKETRGGVRVLREGRVEVSFDFGATWGTICSTSWSMREANVVCRQLGLGFASKATQNTEHGDNRRYPWAMVGTLCRGTERRLAECTRESRYPNICDARNRNITVVACVRHSADLEIGLADIERTARLESVPMSRLTCAMEEHCLSADAYVIRQTKPHAERILLRFSVKATNVGNADVSPYANYKDWVWHQCHRHYHSMNVFATFDVYDLNYKKVAQGHKASFCLMDTECKPGFSRKYTCGNTTQGISVGCSDTYTDVLDCQWVDVTRVPVNRRYILRVALNPEYKLGEISFENNGAECVLDYTGVHETTRIFNCRRSPLWFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMQ9</accession>
    <name>A0A6I8UMQ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4800790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358003.1">
        <property type="nucleotide sequence ID" value="XM_001357966.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMQ9"/>
    <dbReference type="GeneID" id="4800790"/>
    <dbReference type="KEGG" id="dpo:4800790"/>
    <dbReference type="InParanoid" id="A0A6I8UMQ9"/>
    <dbReference type="OMA" id="KFLVFRL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071671">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001358003.1"/>
        </source>
    </evidence>
    <sequence length="94" mass="10867" checksum="7774A6F353D7E8BC" modified="2020-08-12" version="1">MLRHKRNCHAIQTPSDDEHVKLSAPIDALHPLPTQSQSLPLPPLRQPLLPLIQLQRLNQRVTFSLELYHKNVIKKLPALKQHEVYKLTKNISQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMU4</accession>
    <name>A0A6I8UMU4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Deoxyribonuclease TATDN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4800859</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Deoxyribonuclease which catalyzes (in vitro) the decatenation of kinetoplast DNA, which are circular DNA catenated to each other, producing linear DNA molecules. Plays an important role in chromosomal segregation and cell cycle progression during eye development probably via its DNA decatenation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358059.1">
        <property type="nucleotide sequence ID" value="XM_001358022.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMU4"/>
    <dbReference type="FunCoup" id="A0A6I8UMU4">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="GeneID" id="4800859"/>
    <dbReference type="KEGG" id="dpo:4800859"/>
    <dbReference type="InParanoid" id="A0A6I8UMU4"/>
    <dbReference type="OMA" id="HDAKSWE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077276">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000018">
        <property type="entry name" value="3'-5' ssDNA/RNA exonuclease TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050891">
        <property type="entry name" value="TatD-type_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060:SF15">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060">
        <property type="entry name" value="TATD FAMILY DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358059.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="36274" checksum="BE9AFA3CFCB8A99B" modified="2020-08-12" version="1">MEKQQQQTAPEVAATPAPLLEEQLDMDDSMKHCFENLIVIDVGANLTNKKYSRDLDSVVQRARDAGIQKLMVHGTSVKSSKEALRLSRIYPDIIYSTAGIHPHDSKSIVEEPSTWFDLEHIAQAQECVAVGPCGLDYQRDFSEPDAQKQIFAKQLHLAIRLNKPVLIHERSAQKDLLEILDKFENLPPVIIRGFMGTAEEALKYLDRRFYISLTGYLCKDKSDTGVRRLLENGTLPLDRLLVETDAPFMYPNTRASKLPQHVKTAITERSLLYLHRYCTFQRNEPCSLPAIVEMIAAFMKKTPDEVALATAFNALKLFGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMX1</accession>
    <name>A0A6I8UMX1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tetraspanin-31-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Tsp97E</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357951.3">
        <property type="nucleotide sequence ID" value="XM_001357914.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMX1"/>
    <dbReference type="FunCoup" id="A0A6I8UMX1">
        <property type="interactions" value="324"/>
    </dbReference>
    <dbReference type="GeneID" id="4800733"/>
    <dbReference type="KEGG" id="dpo:4800733"/>
    <dbReference type="InParanoid" id="A0A6I8UMX1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079508">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF452">
        <property type="entry name" value="LD03691P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001357951.3"/>
        </source>
    </evidence>
    <sequence length="230" mass="25323" checksum="A756B2F3BEFAAEE0" modified="2020-08-12" version="1">MCGGFTCSKNALIALNILYVMIGFLLIGVGVYARAASIVTNLPIVGGILACGVILICISMLGLAGAVKHHQVMLFFYMIILFMLFLIQFSIASSCLAVNSEQQQQFAEQGWMTVPPELRQQVQESLHCCGFNATSSINLPTSPLAPTDEPSCELVNRKCCEQSNDVDCHCPACGPMLEDKIDYAFKLCGGLGIFFSFTEVLAVFLARRYRNQHDPCYLPARAVFPHNYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ88</accession>
    <name>A0A6I8UQ88_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Arl6IP1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359060.1">
        <property type="nucleotide sequence ID" value="XM_001359023.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ88"/>
    <dbReference type="FunCoup" id="A0A6I8UQ88">
        <property type="interactions" value="1090"/>
    </dbReference>
    <dbReference type="GeneID" id="4802067"/>
    <dbReference type="KEGG" id="dpo:4802067"/>
    <dbReference type="InParanoid" id="A0A6I8UQ88"/>
    <dbReference type="OMA" id="LAFFMDF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070304">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052114">
        <property type="entry name" value="ER_autophagy_membrane_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952:SF0">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952">
        <property type="entry name" value="ADP-RIBOSYLATION-LIKE FACTOR 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="7">
        <location>
            <begin position="29"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359060.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="22515" checksum="5908F73A4AA8AFE0" modified="2020-08-12" version="1">MTTSQVDQKRALNKLKHDLEPFRTAIVSAYGVLTWEKNYYPGVVFGSISVIFLVLWYMDLSVITMLSLLALLTTVLDYVFPTISRLLFSGVNWDGDHEAKFEDVCGQMYNIKANLVVWYEYLFKERKSTVFVIMIGVFLVALAWIGAIVNNLLLMYLGTLLIAMWPGLQEKDVFKGITKKASKIINEKIQCGKRKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ91</accession>
    <name>A0A6I8UQ91_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Charged multivesicular body protein 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Vps2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359168.3">
        <property type="nucleotide sequence ID" value="XM_001359131.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ91"/>
    <dbReference type="FunCoup" id="A0A6I8UQ91">
        <property type="interactions" value="2153"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802201"/>
    <dbReference type="InParanoid" id="A0A6I8UQ91"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="207"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359168.3"/>
        </source>
    </evidence>
    <sequence length="256" mass="27336" checksum="58FDD64C9635798F" modified="2020-08-12" version="1">MDWLFGKKISPDEMLRKNQRALNKAMRDLDRERMKMEQQEKKIIADIKKMAKEGQMDAVKIMAKDLVRTRRYAKKFMLMKANIQAVSLKIQTLKSQNTMAQAMKGVTKAMQNMNRQLNLPQIQKILQDFEKQSEMMDMKEEMINDAIDDAMEDEGDEEETDAVVSQVLDELGLQLGEQLGDLPNASGSLSIAGGVGAQKTPAAVAAGGVGAGGAGGGGGGGGSGAGGGAAGGSGGTSPMSDADADLQARLDKLRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQZ5</accession>
    <name>A0A6I8UQZ5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">trypsin</fullName>
            <ecNumber evidence="11">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4802610</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001359499.4">
        <property type="nucleotide sequence ID" value="XM_001359462.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQZ5"/>
    <dbReference type="FunCoup" id="A0A6I8UQZ5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802610"/>
    <dbReference type="InParanoid" id="A0A6I8UQZ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000036">
        <property type="entry name" value="Trypsin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="63"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001359499.4"/>
        </source>
    </evidence>
    <sequence length="298" mass="32143" checksum="4D3FE5AEC27D9F8E" modified="2020-08-12" version="1">MGGYHFQLIRVGVKVPPALVMFAIKLFCLYLSVTMAATWIAPTKTSTDPMRSMETAAADPIRINGGQMVNETVPFQISLQAQRRGRWSHFCGGSIVSAQHVLTAAHCVEKMKVENISVVVGTLNWKSGGLRHRVVAKHVHPQYAMSPRIINDIALVKVTPPFRLNRADIGTIHLGGTDRIGEKVAVRLTGWGSTTPATSSASLPDHLQALHYSTISNEECNQKGFRVTRNEICALATHGQGACVGDSGGPLILAGSRPQLVGIVSYGSSTCAQGRPDVYTRVSSFLPYISKVINQDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR42</accession>
    <name>A0A6I8UR42_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CSN5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M67A family. CSN5 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359383.1">
        <property type="nucleotide sequence ID" value="XM_001359346.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR42"/>
    <dbReference type="FunCoup" id="A0A6I8UR42">
        <property type="interactions" value="2904"/>
    </dbReference>
    <dbReference type="GeneID" id="4802468"/>
    <dbReference type="KEGG" id="dpo:4802468"/>
    <dbReference type="InParanoid" id="A0A6I8UR42"/>
    <dbReference type="OMA" id="VKMKLFQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073358">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000203">
        <property type="entry name" value="COP9 signalosome complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040961">
        <property type="entry name" value="CSN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18323">
        <property type="entry name" value="CSN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0736">Signalosome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="13">
        <location>
            <begin position="52"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001359383.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="37049" checksum="741B7C075C624B0A" modified="2020-08-12" version="1">MDVDAAQKTWELENNIQTLPSCDEIFRYDAEQQRQIIDAKPWEKDPHFFKDIKISALALLKIVMHARSGGTLEVMGLMLGKVEDNTMIVMDAFALPVEGTETRVNAQAQAYEYMTAYMEAAKEVGRMEHAVGWYHSHPGYGCWLSGIDVSTQMLNQTYQEPFVAIVVDPVRTVSAGKVCLGAFRTYPKGYKPPNEEPSEYQTIPLNKIEDFGVHCKQYYPLEISYFKSALDRKLLDSLWNKYWVNTLGSSGLLTNTEYTTGQIMDLSEKLEQSENFLGRGTDVNEKRSEDKLSKATRDCSRSTIELIHGLMAQIVKDKLFNKVGLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URL6</accession>
    <name>A0A6I8URL6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">protein fer3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4802845</name>
        <name evidence="8 9" type="synonym">Fer3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <strain evidence="7 8">MV2-25</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359681.1">
        <property type="nucleotide sequence ID" value="XM_001359644.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232460.1">
        <property type="nucleotide sequence ID" value="XM_033376569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URL6"/>
    <dbReference type="GeneID" id="4802845"/>
    <dbReference type="KEGG" id="dpo:4802845"/>
    <dbReference type="OMA" id="FASPYNH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079948">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11415">
        <property type="entry name" value="bHLH_TS_FERD3L_NATO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000035">
        <property type="entry name" value="Pancreas-specific transcription factor 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF63">
        <property type="entry name" value="FER3-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="88"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="57"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="147"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033232460.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23162" checksum="CCDE8A8A213A06A0" modified="2020-08-12" version="1">MQHPHPSDQPTYMPDVPFQPLWGQEAPPPPPIVPYQELIAGFPCTDLSLWQRSQVAPLVPQRPSTHGRSNGSSSSSSKKTRRRVASMAQRRAANIRERRRMFNLNEAFDKLRRKVPTFAYEKRLSRIETLRLAITYIGFMAELLSGTPSNSHSHSHSHSKSRSDVYPGMNGHHQAAPPSMHPPHHLHPAAAYHRDFASPYSHSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URM3</accession>
    <name>A0A6I8URM3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC4803005</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol = cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42816"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:82751"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:42817"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a sterol + a long-chain fatty acyl-CoA = a long-chain 3-hydroxysterol ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:59816"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:232093"/>
            <dbReference type="EC" id="2.3.1.26"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:59817"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>cholesterol + (9Z)-octadecenoyl-CoA = cholesteryl (9Z-octadecenoate) + CoA</text>
            <dbReference type="Rhea" id="RHEA:41436"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:46898"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:41437"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an acyl-CoA + cholesterol = a cholesterol ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:17729"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17002"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:17730"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Endoplasmic reticulum membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359810.2">
        <property type="nucleotide sequence ID" value="XM_001359773.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URM3"/>
    <dbReference type="FunCoup" id="A0A6I8URM3">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803005"/>
    <dbReference type="InParanoid" id="A0A6I8URM3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034736">
        <property type="term" value="F:cholesterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030687">
        <property type="entry name" value="Sterol_acyltranf_meta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF8">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500230">
        <property type="entry name" value="Sterol_acyltranf_ACAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="7 17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="341"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="519"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="70"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001359810.2"/>
        </source>
    </evidence>
    <sequence length="565" mass="66256" checksum="86FD60C1070F164C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT60</accession>
    <name>A0A6I8UT60_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">F-box/LRR-repeat protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Ppa</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360491.3">
        <property type="nucleotide sequence ID" value="XM_001360454.4"/>
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    </dbReference>
    <dbReference type="KEGG" id="dpo:4803838"/>
    <dbReference type="InParanoid" id="A0A6I8UT60"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22125">
        <property type="entry name" value="F-box_FBXL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000059">
        <property type="entry name" value="Partner of Paired"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000546">
        <property type="entry name" value="Partner of Paired"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000569">
        <property type="entry name" value="Partner of Paired"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000634">
        <property type="entry name" value="Partner of Paired"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047932">
        <property type="entry name" value="FBXL14_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF190">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="137"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360491.3"/>
        </source>
    </evidence>
    <sequence length="529" mass="57626" checksum="1380FA97D471495E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTA6</accession>
    <name>A0A6I8UTA6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4803946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360585.3">
        <property type="nucleotide sequence ID" value="XM_001360548.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTA6"/>
    <dbReference type="FunCoup" id="A0A6I8UTA6">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803946"/>
    <dbReference type="InParanoid" id="A0A6I8UTA6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UTA6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026127754" evidence="2">
        <location>
            <begin position="22"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360585.3"/>
        </source>
    </evidence>
    <sequence length="224" mass="25584" checksum="C5C59F37302C4CE6" modified="2020-08-12" version="1">MFSRQQLMLICLGLGVLGCLSLPIEKPADIEEDVISIDNQPQKVIRVHPQDLPSKKDHTGANMNSALFQIQTLRPVTSTGNRKYVDSKQMRMRMRKRRPRPATDGEDSSQPIQALKQKELKAFPKQKTKQQKQLKFIPPQTDAEEYLNGDMDMDTLKDMASQLLPVQMTPGPYPVYYVVSKTNGRFGKFPIKAFGSPAEFSKYLVKSKAEPISRHQRFEGYVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTT1</accession>
    <name>A0A6I8UTT1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">AQP</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family. AQP11/AQP12 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360767.2">
        <property type="nucleotide sequence ID" value="XM_001360730.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTT1"/>
    <dbReference type="FunCoup" id="A0A6I8UTT1">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="GeneID" id="4804163"/>
    <dbReference type="KEGG" id="dpo:4804163"/>
    <dbReference type="InParanoid" id="A0A6I8UTT1"/>
    <dbReference type="OMA" id="EHFTVYW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071560">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UTT1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000018">
        <property type="entry name" value="Aquaporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051883">
        <property type="entry name" value="AQP11/12_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016697">
        <property type="entry name" value="Aquaporin_11/12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21191">
        <property type="entry name" value="AQUAPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21191:SF16">
        <property type="entry name" value="AQUAPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017529">
        <property type="entry name" value="Aquaporin_11/12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360767.2"/>
        </source>
    </evidence>
    <sequence length="273" mass="29948" checksum="E449297A580FFDC8" modified="2020-08-12" version="1">MSMASLGVSGIFMVGCCVAAQIARLVSRRLVTREGIVPILVNEAIAAAELCACCFELIIVADNFGVAAYAVCLFVLTVWWGKVWGDASACPYTHMEDVLEGRTSFKEMALRTWAELMGGCCVYRIVQVFWWLEFAETHKGRAFESCSADLQVSPYLGAVIEGVATLLCRLASKTLSDKEPKFASYIDSFIGTSLVVAVYAFNYALLAAFNFSGGYFNPVLATALKWGCRGHTNFEHIIVYWIGACVGAVLSVPIYKLPIVRRFLLGEDKPKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV01</accession>
    <name>A0A6I8UV01_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ragulator complex protein LAMTOR2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Lamtor2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361320.3">
        <property type="nucleotide sequence ID" value="XM_001361283.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV01"/>
    <dbReference type="FunCoup" id="A0A6I8UV01">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804818"/>
    <dbReference type="InParanoid" id="A0A6I8UV01"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000004">
        <property type="entry name" value="ragulator complex protein LAMTOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="LATE ENDOSOMAL/LYSOSOMAL MP1 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="3">
        <location>
            <begin position="7"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00072715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361320.3"/>
        </source>
    </evidence>
    <sequence length="125" mass="13482" checksum="0AC55507DD96EA45" modified="2020-08-12" version="1">MLKPKALTQVLSQANTGGVENTLLLSQEGALLAYSGYGDKDARITAAIASNIWAAYEKHGRNAFREDRLTFVLIDCETGHVAITQVASVLLCLYARSQVGLGLLKQKAMSLAAYLERPLKQISAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVB9</accession>
    <name>A0A6I8UVB9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial-processing peptidase subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alpha-MPP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Inactive zinc metalloprotease alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC4805000</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of PMPCA (alpha) and PMPCB (beta) subunits, forming the mitochondrial processing protease (MPP) in which PMPCA is involved in substrate recognition and binding and PMPCB is the catalytic subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361470.2">
        <property type="nucleotide sequence ID" value="XM_001361433.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVB9"/>
    <dbReference type="FunCoup" id="A0A6I8UVB9">
        <property type="interactions" value="2718"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805000"/>
    <dbReference type="InParanoid" id="A0A6I8UVB9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000010">
        <property type="entry name" value="Mitochondrial-processing peptidase alpha subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000014">
        <property type="entry name" value="Mitochondrial-processing peptidase alpha subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF49">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="14">
        <location>
            <begin position="104"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="15">
        <location>
            <begin position="258"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001361470.2"/>
        </source>
    </evidence>
    <sequence length="555" mass="60827" checksum="2DCF80A63AB8199B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVE0</accession>
    <name>A0A6I8UVE0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PAN2-PAN3 deadenylation complex catalytic subunit PAN2</fullName>
            <ecNumber evidence="9">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Poly(A)-nuclease deadenylation complex subunit 2</fullName>
            <shortName evidence="9">PAN deadenylation complex subunit 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">PAN2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 metal cations per subunit in the catalytic exonuclease domain.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Positively regulated by the regulatory subunit PAN3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
            <location evidence="9">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">Shuttles between nucleus and cytoplasm.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C19 family. PAN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="9"/>
    <dbReference type="RefSeq" id="XP_001361501.2">
        <property type="nucleotide sequence ID" value="XM_001361464.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVE0"/>
    <dbReference type="FunCoup" id="A0A6I8UVE0">
        <property type="interactions" value="1620"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805038"/>
    <dbReference type="InParanoid" id="A0A6I8UVE0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UVE0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010606">
        <property type="term" value="P:positive regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06143">
        <property type="entry name" value="PAN2_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000421">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000011">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03182">
        <property type="entry name" value="PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030843">
        <property type="entry name" value="PAN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050785">
        <property type="entry name" value="PAN2-PAN3_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048841">
        <property type="entry name" value="PAN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028881">
        <property type="entry name" value="PAN2_UCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728">
        <property type="entry name" value="DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728:SF0">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20770">
        <property type="entry name" value="PAN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13423">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="11">
        <location>
            <begin position="504"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="813"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="857"/>
            <end position="878"/>
        </location>
    </feature>
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        <source>
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    <sequence length="1237" mass="138549" checksum="206E88C5B372C1BD" modified="2020-08-12" version="1"> </sequence>
</entry>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <dbReference type="RefSeq" id="XP_001361278.3"/>
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            <begin position="1450"/>
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        <location>
            <begin position="1532"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133889.3"/>
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    </evidence>
    <sequence length="1862" mass="218256" checksum="92AEED3F6FD73D01" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZG4</accession>
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        <submittedName>
            <fullName evidence="4">Protein FAM98B</fullName>
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        <name evidence="4" type="primary">LOC6901391</name>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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            <dbReference type="RefSeq" id="XP_002134079.3"/>
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    <sequence length="115" mass="10375" checksum="8A2B0D9097A149B3" modified="2020-08-12" version="1">MRYTLLVVFLCGLLFAFVSAGGGGQGGWQKSGGGGGGQGGWQKSGGGGGGQGGWQQGGGGGGGQGGWQKSGGGGGGQGGWQKSGGGGGGQGGWQQGGGGGGKHGQGGAKHGGGGW</sequence>
</entry>
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            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_002134433.2"/>
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</entry>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <dbReference type="RefSeq" id="XP_002134965.2"/>
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    <sequence length="168" mass="18035" checksum="27D340DC8918C59F" modified="2020-08-12" version="1">MRLLPVLALTLAILVCGTVVAAQDTTDSSEYDYSDYNDDDYSYEDYEDEGSTEDATTEASSNNDSGVSSGEESEDSSEDQVDSGSSEVTPAPVVPTKPSPATPKKAPKRAAGKNKQRRRRTTAAPKRRTTVAPKRRQPVATPTRRQTPVAAAKKRATVAAKKRKQASG</sequence>
</entry>
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        <submittedName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <property type="entry name" value="OS12G0136300 PROTEIN"/>
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            <begin position="1"/>
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        <location>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_002134634.2"/>
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    </evidence>
    <sequence length="1215" mass="134214" checksum="CA403001FA761952" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1Q7</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="6">HTH OST-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6896718</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002136932.2">
        <property type="nucleotide sequence ID" value="XM_002136896.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1Q7"/>
    <dbReference type="FunCoup" id="A0A6I8V1Q7">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896718"/>
    <dbReference type="InParanoid" id="A0A6I8V1Q7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030719">
        <property type="term" value="P:P granule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09972">
        <property type="entry name" value="LOTUS_TDRD_OSKAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF29">
        <property type="entry name" value="FI02030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="HTH OST-type" evidence="6">
        <location>
            <begin position="6"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="176"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002136932.2"/>
        </source>
    </evidence>
    <sequence length="471" mass="54180" checksum="3BA2C0A8808C9B44" modified="2020-08-12" version="1">MDTSGELGVLKKMVHSVLVSSPERMTLQKLSRDYKQMVGSEVPHWQFGYTDVETFLRSMPDVIKVIGSGPMAIVQPLSTAKSAHIQNFVNNQKKISRKSRIPAAQKPSDLVFINEKKALSGPVYNLVNNFQPHMLPNSQQARVKNAQTTHPIDPIYSAQMRKMFDMHKYLYKNSNQNQVQRPHPRCNSTDNDPSGQTKKKEKNVSVAKQMPIEPYRSDDREVLPNFDQLKVDVSFGDDCNESDAFPEYAVDQRVLSFDYPRDAVRSDYKLPRRVLEKTLKVDERYFLQLVEVTNPHSFHFWIYDDYDLYDNFTHNMQQAYKNLDSATFTMPQCLMTPGHLCVVCPINSSEWERAKVVSHRKNNLRKTIKVELIDTGVIDTVYVKDVKFLMKEFAKLPPQGMQGRLAYVAPMSSRNWSSKAVNAFKNQVNNRRLFGKVEAIKHNIAHMVLVGTDNVNLNRSLIDSGLARRWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2U8</accession>
    <name>A0A6I8V2U8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cystatin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6897326</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137478.2">
        <property type="nucleotide sequence ID" value="XM_002137442.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2U8"/>
    <dbReference type="KEGG" id="dpo:6897326"/>
    <dbReference type="InParanoid" id="A0A6I8V2U8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053128">
        <property type="entry name" value="Cystatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319:SF2">
        <property type="entry name" value="CYSTATIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12319">
        <property type="entry name" value="CYSTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026094293" evidence="2">
        <location>
            <begin position="20"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="3">
        <location>
            <begin position="20"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002137478.2"/>
        </source>
    </evidence>
    <sequence length="122" mass="13259" checksum="395E9C5FD3121542" modified="2020-08-12" version="1">MHASKVIFVLGLVLAVAFAGYAGRPRTLEGDDLKEAEQLLSTTLSQLATGDGPSYQLVNLISATSQVVSGTLRTYKVELSDGSDKKQCTVKIWSQPWLEESGKGINIKVQCEGDEAELDRTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2X3</accession>
    <name>A0A6I8V2X3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phosphatidylinositol N-acetylglucosaminyltransferase subunit P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PIG-P</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137656.1">
        <property type="nucleotide sequence ID" value="XM_002137620.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2X3"/>
    <dbReference type="FunCoup" id="A0A6I8V2X3">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="GeneID" id="6897507"/>
    <dbReference type="KEGG" id="dpo:6897507"/>
    <dbReference type="InParanoid" id="A0A6I8V2X3"/>
    <dbReference type="OMA" id="PVEFGLH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248713">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="6">
        <location>
            <begin position="11"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137656.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="17943" checksum="2A832BE41F5185A5" modified="2020-08-12" version="1">MPEHSPAPTPHRAIYGFAFYMLFTVLFIVYVAWALLPFEFGLQPYLPDKYFAVFVPFLVLMFAWFFAFLIYPAINMSLTVNVDSIHSVVDPKLALPKGTAFTSWSQLKNSKDSPTKQKKPTPINCNMCKTSHKPITRAPIAPLRFLDLQEVNTAYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3F5</accession>
    <name>A0A6I8V3F5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phospholipase A1 VesT1.02-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6897058</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137242.2">
        <property type="nucleotide sequence ID" value="XM_002137206.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3F5"/>
    <dbReference type="FunCoup" id="A0A6I8V3F5">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897058"/>
    <dbReference type="InParanoid" id="A0A6I8V3F5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000076">
        <property type="entry name" value="phospholipase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002334">
        <property type="entry name" value="Allerg_PlipaseA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF150">
        <property type="entry name" value="FI01825P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00825">
        <property type="entry name" value="DOLALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026209067" evidence="5">
        <location>
            <begin position="17"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="61"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137242.2"/>
        </source>
    </evidence>
    <sequence length="339" mass="36826" checksum="3E6B05749021C8E1" modified="2020-08-12" version="1">MKVLFVLAAVLAAVSALPVEERINGENGWFIPKIDGTFEWMDLKDAEELLSRGDQMEGRISTNAVKFYLYTRSNPTDGKEIKSDASSIDGSHFNKDHGTRFVIHGWTQSAQDDMNTRITKAWLSRGDYNVIVVDWARARSVDYASSVLAVSGAGAKVGEMIKYLHEHHGMSLDSLEVIGHSLGAQVSGYAGKTVGKGRIHSIVGLDPALPLFSYDKPDKRLSSDDAHYVESIQTNGGKLGFLKPIGKGAFYPNGGKKQPGCGVDVTGSCSHGRSVLYYAEAVTEDNFGTIKCHDYEDAVSKKCGSTYSSVRMGAVTNAYMVDGDYYVPVNSKAPFGKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V452</accession>
    <name>A0A6I8V452_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MICOS complex subunit MIC13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138472.1">
        <property type="nucleotide sequence ID" value="XM_002138436.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V452"/>
    <dbReference type="FunCoup" id="A0A6I8V452">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="GeneID" id="6898430"/>
    <dbReference type="KEGG" id="dpo:6898430"/>
    <dbReference type="InParanoid" id="A0A6I8V452"/>
    <dbReference type="OMA" id="FHVVHAM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246173">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15816" checksum="0B473ACC5ECF9755" modified="2020-08-12" version="1">MASQIITGVRGLFTAAKGLFHVVHAMDQGSFDAEALAPSPEAEGKFVAHYKCNLAIQRGMYFALRMPDWASEFKKQTHDILATTLKHHVDLPKEKPNNASGSQKKLRGCFYEEQLHSPPEESKPIRSPKIKDDITCGKRVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V476</accession>
    <name>A0A6I8V476_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein NRDE2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6898464</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRDE2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138507.2">
        <property type="nucleotide sequence ID" value="XM_002138471.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V476"/>
    <dbReference type="FunCoup" id="A0A6I8V476">
        <property type="interactions" value="1111"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898464"/>
    <dbReference type="InParanoid" id="A0A6I8V476"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902369">
        <property type="term" value="P:negative regulation of RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013633">
        <property type="entry name" value="NRDE-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13471:SF0">
        <property type="entry name" value="NUCLEAR EXOSOME REGULATOR NRDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13471">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08424">
        <property type="entry name" value="NRDE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002138507.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="996" mass="115003" checksum="3212C272FCAF3FAC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4P5</accession>
    <name>A0A6I8V4P5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial sodium/calcium exchanger protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6898772</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138749.2">
        <property type="nucleotide sequence ID" value="XM_002138713.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4P5"/>
    <dbReference type="FunCoup" id="A0A6I8V4P5">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="GeneID" id="6898772"/>
    <dbReference type="KEGG" id="dpo:6898772"/>
    <dbReference type="InParanoid" id="A0A6I8V4P5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245628">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051359">
        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="477"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="565"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="591"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="9">
        <location>
            <begin position="95"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="9">
        <location>
            <begin position="458"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002138749.2"/>
        </source>
    </evidence>
    <sequence length="626" mass="71550" checksum="3AA17B496E8A4E96" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V500</accession>
    <name>A0A6I8V500_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC6898089</name>
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    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138174.1">
        <property type="nucleotide sequence ID" value="XM_002138138.3"/>
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        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="GeneID" id="6898089"/>
    <dbReference type="KEGG" id="dpo:6898089"/>
    <dbReference type="InParanoid" id="A0A6I8V500"/>
    <dbReference type="OMA" id="KDQYWFK"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006601">
        <property type="entry name" value="Uncharacterised_DM11_DROME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00675">
        <property type="entry name" value="DM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026360384" evidence="1">
        <location>
            <begin position="18"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138174.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="23016" checksum="67948BE1C57B08C2" modified="2020-08-12" version="1">MWRILFLIHVGLAGIWATDYELLLEDPDIFAPCTEARPGTMFIREAINFDKLTVEQDPDTIHISGNATTNWDLQPNDRIAAKFALFHYQRGNWEPTVFTMATQDFCAIMYDKDQYWFKYWTTFITNRQEVEQDCIKKAGTTLIHEPFHLRLMLTNIRGATLHGRYKTVISFEAFDEKNVPRPNSVCFEIRGEAEKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5W6</accession>
    <name>A0A6I8V5W6_DROPS</name>
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        <submittedName>
            <fullName evidence="6">Meiosis-specific nuclear structural protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC13036532</name>
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    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_003736713.2">
        <property type="nucleotide sequence ID" value="XM_003736665.3"/>
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        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:13036532"/>
    <dbReference type="InParanoid" id="A0A6I8V5W6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043596">
        <property type="entry name" value="CFAP53/TCHP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183">
        <property type="entry name" value="TRICHOPLEIN KERATIN FILAMENT-BINDING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="183"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_003736713.2"/>
        </source>
    </evidence>
    <sequence length="533" mass="63643" checksum="B0F1BD2D5ADFFF61" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7M0</accession>
    <name>A0A6I8V7M0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tafazzin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Taz</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:33731"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64743"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33732"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:33733"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:43816"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43817"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:43818"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the taffazin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_004444285.2">
        <property type="nucleotide sequence ID" value="XM_004444228.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V7M0"/>
    <dbReference type="FunCoup" id="A0A6I8V7M0">
        <property type="interactions" value="978"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V7M0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V7M0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000872">
        <property type="entry name" value="Tafazzin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497:SF0">
        <property type="entry name" value="TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497">
        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00979">
        <property type="entry name" value="TAFAZZIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="15">
        <location>
            <begin position="193"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="52"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_004444285.2"/>
        </source>
    </evidence>
    <sequence length="390" mass="45092" checksum="955CCAE19F58FE72" modified="2020-08-12" version="1">MLMAVCTKLCGLRHVRSAAASATRRFYWELVPLASANGPHRSIFALSARQYQQAPETRAEPDERSTAGLHKQEQTEQRPALSRNSSPTVFSNQQIQPQPPSQPPAQPPPQPPTMSDRDPRMNMPTAVQIPYNIDWIFQKQRCPSRVWWLASQFVITAVGIFSKILLMLMNKTRVYNKERLVDMISKRPQDVPLLTVSNHYSCFDDPGIWGILPLRQACSTNRIRWSMAAHDICFTNKYHSMFFMFGKCIPVVRGSGVYQEAINLCIEKCAAGQWVHVFPEGKVNMEKQEIRLKWGVGRIIYESPKMPIILPMWHEGMDDVLPNVEPYVLQWRKKVTINIGQPMDLNDFVDDLKKRQVPEPTARKLITDKIQEEFRILRMETEKLHRERQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8K0</accession>
    <name>A0A6I8V8K0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein rolling stone isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035434.2">
        <property type="nucleotide sequence ID" value="XM_015179948.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V8K0"/>
    <dbReference type="FunCoup" id="A0A6I8V8K0">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V8K0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V8K0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049352">
        <property type="entry name" value="Rost"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242:SF46">
        <property type="entry name" value="IP08657P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242">
        <property type="entry name" value="OS02G0130600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21534">
        <property type="entry name" value="Rost"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015035434.2"/>
        </source>
    </evidence>
    <sequence length="276" mass="32362" checksum="5C91DA0F3DCC0F66" modified="2020-08-12" version="1">MGENKNLIKNDGETVPHQQPLSLSEELRRENFGLQHHAPEDFLRSQWQSGLTSRWFLGYRWLLGIFFGTGVISCLAKYHHNGRWFIYLTDWGFLLCGITSVSGAILVTIHHWNPQRMGERFRNNWQIKAYWVCYWINLIFANVIALVYWTCIYPQDRDPSNPAYFTDLYNIWTHALPPIFFSVDHLIVAQPTRLLHFVYPLAFGWLYTGFTFIYYVMGGLDLKGRRYIYNVLNYSKPREALFTIGAISALSVVVSTLHYGVYRMRTFLARKLGKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V957</accession>
    <name>A0A6I8V957_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcription initiation factor TFIID subunit 5-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC26533476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036414.2">
        <property type="nucleotide sequence ID" value="XM_015180928.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V957"/>
    <dbReference type="InParanoid" id="A0A6I8V957"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V957">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="228"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036414.2"/>
        </source>
    </evidence>
    <sequence length="388" mass="43539" checksum="EE859E60BB45173B" modified="2020-08-12" version="1">MNHPEINFVLKQNQRPTSGPMEGAQKESPKANIFLCTVAALNEQVVTAIFSDRLCMMALGTSDSVIYMFELNLIVSRSNGSEVDEVGMDPSNSDDQFTLRGLEGPLYDGAFCQKCKYFVSCAVDFTVRLWCLSSRCILFNYIGHSWPIRSVVFTANGLFATASHSGIVTISKMDSIKPYRQFSAANNEPLTACLLHPAAYTYYCIVGCLNGAIRIWDYAGQCFEWRVFRGHKSAVTVLALSNCTFYLASGDASHITLVWDVRYQWVIRSLGQHLGPIRSIDFSLDNNLLVIGAGDKYRQLSIWDFKSLKQAEPQKKACNVSPLSNEHLLVFSHVNVAAPIAKIRFITYDILMGVCVDIPHVPWRNRSQISWIDRKCQMNAFLNQKIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAC3</accession>
    <name>A0A6I8VAC3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vasotab</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC26532915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037088.2">
        <property type="nucleotide sequence ID" value="XM_015181602.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232615.1">
        <property type="nucleotide sequence ID" value="XM_033376724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAC3"/>
    <dbReference type="KEGG" id="dpo:26532915"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634492" description="Vasotab" evidence="1">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015037088.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033232615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9351" checksum="BF4BD4031901654D" modified="2020-08-12" version="1">MKLIMILALILALMALVTAQECNLPCNRRIEYLCGRTVRNGSEIECTFRNPCAMDRHGCEKREVWRKTSTGRCTRDSDACGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBQ7</accession>
    <name>A0A6I8VBQ7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="26">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31 32" type="primary">ATP8B</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="31 32" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="31 32">MV-25-SWS-2005</strain>
            <tissue evidence="31 32">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 26">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="22">Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit ATP8B1 and an accessory beta subunit TMEM30A. The flippase ATP8B1:TMEM30A complex can form an intermediate phosphoenzyme in vitro. Also interacts with beta subunit TMEM30B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Membrane</location>
            <topology evidence="26">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 26">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_033380535.1"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:apical plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:cell projection"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
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    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0140327">
        <property type="term" value="F:flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
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    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
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    </dbReference>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
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    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
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        <property type="entry name" value="HAD-like_sf"/>
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        <property type="entry name" value="HAD_sf"/>
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    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
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        <property type="entry name" value="P-type_ATPase_N"/>
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    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
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        <property type="entry name" value="P_typ_ATPase_c"/>
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        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
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        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR24092:SF190">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 24 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 25 26" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 24 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 26" id="KW-1278">Translocase</keyword>
    <keyword evidence="9 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="437"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="486"/>
            <end position="515"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1393"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1443"/>
            <end position="1462"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1474"/>
            <end position="1492"/>
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    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1504"/>
            <end position="1527"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1547"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="28">
        <location>
            <begin position="180"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="29">
        <location>
            <begin position="1329"/>
            <end position="1575"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="21"/>
            <end position="59"/>
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    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="74"/>
            <end position="104"/>
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    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="123"/>
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    <feature type="region of interest" description="Disordered" evidence="27">
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            <begin position="602"/>
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    <feature type="region of interest" description="Disordered" evidence="27">
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            <begin position="642"/>
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    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="768"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
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            <end position="1610"/>
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    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1661"/>
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    </feature>
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        <location>
            <begin position="133"/>
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            <begin position="1666"/>
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            <position position="920"/>
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        <ligand>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00052223"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00062467"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
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    <evidence type="ECO:0000256" key="24">
        <source>
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    <evidence type="ECO:0000256" key="25">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF16209"/>
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    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_015037937.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_033236426.1"/>
        </source>
    </evidence>
    <sequence length="1727" mass="194059" checksum="2B6BA10661EFB722" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VE20</accession>
    <name>A0A6I8VE20_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PH4alphaNE3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_015038825.2">
        <property type="nucleotide sequence ID" value="XM_015183339.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VE20"/>
    <dbReference type="FunCoup" id="A0A6I8VE20">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896704"/>
    <dbReference type="InParanoid" id="A0A6I8VE20"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VE20">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026170904" description="procollagen-proline 4-dioxygenase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="411"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015038825.2"/>
        </source>
    </evidence>
    <sequence length="536" mass="61175" checksum="123C902DAAEEDF63" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VE90</accession>
    <name>A0A6I8VE90_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MIEF1 upstream open reading frame protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039370.2">
        <property type="nucleotide sequence ID" value="XM_015183884.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VE90"/>
    <dbReference type="KEGG" id="dpo:26533608"/>
    <dbReference type="InParanoid" id="A0A6I8VE90"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272595">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="1">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015039370.2"/>
        </source>
    </evidence>
    <sequence length="68" mass="8334" checksum="A9587ECB37696F91" modified="2020-08-12" version="1">MFQPSRQQILRLYKHLIRYGNHLKLTDKNYFLGRVRHEFRDSRQLTSPSEIEFNFRRGETLLKNGRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VEU5</accession>
    <name>A0A6I8VEU5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">tor</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_015039625.2">
        <property type="nucleotide sequence ID" value="XM_015184139.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VEU5"/>
    <dbReference type="FunCoup" id="A0A6I8VEU5">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VEU5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902533">
        <property type="term" value="P:positive regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000190">
        <property type="entry name" value="Proto-oncogene tyrosine-protein kinase receptor Ret"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF620">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR TORSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026330701" description="receptor protein-tyrosine kinase" evidence="16">
        <location>
            <begin position="22"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="480"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="674"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="895"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="693"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="895"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015039625.2"/>
        </source>
    </evidence>
    <sequence length="940" mass="107234" checksum="3C3D27EC21750D12" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VF41</accession>
    <name>A0A6I8VF41_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Heterogeneous nuclear ribonucleoprotein K isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">HnRNP-K</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <strain evidence="5">MV2-25</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039951.1">
        <property type="nucleotide sequence ID" value="XM_015184465.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039953.1">
        <property type="nucleotide sequence ID" value="XM_015184467.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VF41"/>
    <dbReference type="GeneID" id="6898489"/>
    <dbReference type="OMA" id="KGTEQQI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245734">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22432">
        <property type="entry name" value="KH-I_HNRNPK_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22433">
        <property type="entry name" value="KH-I_HNRNPK_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22434">
        <property type="entry name" value="KH-I_HNRNPK_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000128">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein K, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="93"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="428"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="318"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015039951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015039953.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="50454" checksum="DA5ACA45E9C57334" modified="2020-08-12" version="1">MKREMNDAGDGPQDQKRNRRNEDTVRILIPSSIAGAVIGKGGQHIQKMRTQYKAAVSVDDSQGPERTIQISADIEATLEIITEMLKYFEERDEDFDVRLLIHQSLAGCVIGKGGQKIKEIRDRIGCRFLKVFSNVAPQSTDRVVQTVGKQAQVIEAVREVITLTRDTPIKGAIHNYDPMNFDRVYADEYGGYGTGSGSTRPSQRGGNRNGGAGAAGAGGAAGGGNGGGFGGNAGGGRGSGGGGRGAERFGAGAGGAGVGGVGGGGGMGQRDNPFINPWANGGGGDVDGFGNATGGAGGFAGAGFGGGSGGPFGGGGSFGNNGFGGGPGDFGGNNGSFGQSQGSNSLPSLGNFSQSQGGTNSLPSLGSFGQNQGGNSALGNGLGGGSANNGGVGALGGANGAGGFNAGGGSGGNGSPNAPTNVPQGHDPNNSTQVTIPKELAGAIIGKGGGRIRRIRNESSAYITIDEPLPNSNDRIITISGTPKQIQMAQYLLQQSVHENGRRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFE5</accession>
    <name>A0A6I8VFE5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylamidoglycolate lyase</fullName>
            <ecNumber evidence="1">4.3.2.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Pal1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Peptidyl-alpha-hydroxylglycine alpha-amidating lyase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Mediates the dismutation of the unstable peptidyl(2-hydroxyglycine) intermediate to glyoxylate and the corresponding desglycine peptide amide. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a [peptide]-C-terminal (2S)-2-hydroxyglycine = a [peptide]-C-terminal amide + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:20924"/>
            <dbReference type="Rhea" id="RHEA-COMP:13485"/>
            <dbReference type="Rhea" id="RHEA-COMP:15321"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:137001"/>
            <dbReference type="ChEBI" id="CHEBI:142768"/>
            <dbReference type="EC" id="4.3.2.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:20925"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds one Zn(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.5" evidence="1"/>
    <dbReference type="RefSeq" id="XP_015040133.2">
        <property type="nucleotide sequence ID" value="XM_015184647.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFE5"/>
    <dbReference type="InParanoid" id="A0A6I8VFE5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004598">
        <property type="term" value="F:peptidylamidoglycolate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14958">
        <property type="entry name" value="NHL_PAL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000054">
        <property type="entry name" value="Peptidyl-alpha-hydroxyglycine alpha-amidating lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000720">
        <property type="entry name" value="PHM/PAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10680:SF36">
        <property type="entry name" value="PEPTIDYL-ALPHA-HYDROXYGLYCINE ALPHA-AMIDATING LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10680">
        <property type="entry name" value="PEPTIDYL-GLYCINE ALPHA-AMIDATING MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00790">
        <property type="entry name" value="PAMONOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 20" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 18" id="KW-0732">Signal</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026282263" description="peptidylamidoglycolate lyase" evidence="18">
        <location>
            <begin position="27"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="15">
        <location>
            <begin position="165"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="15">
        <location>
            <begin position="210"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>C-terminal Xaa-(2S)-2-hydroxyglycine residue</name>
            <dbReference type="ChEBI" id="CHEBI:142768"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>C-terminal Xaa-(2S)-2-hydroxyglycine residue</name>
            <dbReference type="ChEBI" id="CHEBI:142768"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>a protein</name>
            <dbReference type="ChEBI" id="CHEBI:16541"/>
        </ligand>
        <ligandPart>
            <name>C-terminal Xaa-(2S)-2-hydroxyglycine residue</name>
            <dbReference type="ChEBI" id="CHEBI:142768"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600720-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600720-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600720-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015040133.2"/>
        </source>
    </evidence>
    <sequence length="562" mass="61986" checksum="1276F3BE57128DAB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFP6</accession>
    <name>A0A6I8VFP6_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">TIL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC26533233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040329.1">
        <property type="nucleotide sequence ID" value="XM_015184843.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFP6"/>
    <dbReference type="GeneID" id="26533233"/>
    <dbReference type="KEGG" id="dpo:26533233"/>
    <dbReference type="InParanoid" id="A0A6I8VFP6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272220">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026050960" description="TIL domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="TIL" evidence="2">
        <location>
            <begin position="26"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015040329.1"/>
        </source>
    </evidence>
    <sequence length="88" mass="10128" checksum="696A7D2501A3A20A" modified="2020-08-12" version="1">MKFPSVPVGIYLMLVSSAIAEELPECVCNDVMVKCVRSCQKLCVDKLYIRKCRYVQCKRGCACTPGMVRKRDITGMCIHRKDCHRWII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VG01</accession>
    <name>A0A6I8VG01_DROPS</name>
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        <submittedName>
            <fullName evidence="7">tRNA (Adenine(37)-N6)-methyltransferase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4805013</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015040364.1">
        <property type="nucleotide sequence ID" value="XM_015184878.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VG01"/>
    <dbReference type="FunCoup" id="A0A6I8VG01">
        <property type="interactions" value="639"/>
    </dbReference>
    <dbReference type="GeneID" id="4805013"/>
    <dbReference type="InParanoid" id="A0A6I8VG01"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071883">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VG01">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.70:FF:000004">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2310.10:FF:000003">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.10">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818:SF0">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="5">
        <location>
            <begin position="95"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="52"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015040364.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="45332" checksum="01CBA8138E0F5322" modified="2020-08-12" version="1">MHEDDLKNQLTIARNEINNLRQQVRNLQHVQRKDIETITRLLKDFRCEGCSSKQNAKAKDGTDTDKAEAAPGLQGQDVNGSGSSNSNSNEESAHFRPIGVIRTAFPEKRAVPRQSVVGSRLRSLIQLNDGVFTNPEHSLEGLSDFSHLWLIYHFHRNNAHPKAKVAPPRLGGERVGVFSTRSPHRPCPIGLSLVEIEKIENATISFFGTDMVDGTPVLDIKPYIPYYDAPALSIDSGMLGAYKPVQPRFYQNFGLVAGRASAGPNETSLDFYDSRQEPDGEESDLELYGAAGYAVNSISSDPILPPPSAVRVPNWVVASHRLSVCFNEHAEAQLKDLQVEKQCIVEILEADPRSVYLRTKYGSQIFTFQLSEVTVTCKFDDKAATVTVVQVRRNENVQVVALDCDQRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VG83</accession>
    <name>A0A6I8VG83_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Oatp58Db</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
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    <dbReference type="RefSeq" id="XP_015040495.1">
        <property type="nucleotide sequence ID" value="XM_015185009.2"/>
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    <dbReference type="FunCoup" id="A0A6I8VG83">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="4805220"/>
    <dbReference type="InParanoid" id="A0A6I8VG83"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077429">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17336">
        <property type="entry name" value="MFS_SLCO_OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF76">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="541"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="577"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="633"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="445"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015040495.1"/>
        </source>
    </evidence>
    <sequence length="686" mass="76638" checksum="0C5F9D3D5E558CC4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHX3</accession>
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        <submittedName>
            <fullName evidence="3">Protein deltex-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015041268.2"/>
        </source>
    </evidence>
    <sequence length="67" mass="7829" checksum="A4923AD5D23D2410" modified="2020-08-12" version="1">MKIRKNGKTRLPKRGGVQRPTENRPTSSNSSGEIDRTSEPLGQQQQHHHQQQQQQQNQCQKEHQMEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VI76</accession>
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        <recommendedName>
            <fullName evidence="1">DUF4777 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="primary">LOC26533778</name>
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    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <begin position="3"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF16007"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015041662.2"/>
        </source>
    </evidence>
    <sequence length="87" mass="10222" checksum="10B542271C404A5F" modified="2020-08-12" version="1">MQRYLYSDHILEAFNVFHRPMVLEEVVAYVAELEVKSMEEVRLDVDNTLTAAWMHGFIKQKDNWYTLDCGYWDYESTDGTAAAQSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VID4</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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        <name evidence="3" type="primary">LOC26533957</name>
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    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Brachycera</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
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        </location>
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        <location>
            <begin position="616"/>
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    </feature>
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        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="635"/>
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        </location>
    </feature>
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        <location>
            <begin position="649"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015041755.2"/>
        </source>
    </evidence>
    <sequence length="658" mass="72804" checksum="466F9A82FBC75B28" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIH8</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC26534283</name>
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    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_015186603.2"/>
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    <dbReference type="KEGG" id="dpo:26534283"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015042089.2"/>
        </source>
    </evidence>
    <sequence length="47" mass="4975" checksum="8499D43974E3626D" modified="2020-08-12" version="1">MWSKIAISGALLVMGGALAASVVDNFAYVDRSLEVAMPKAKRHAAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJK1</accession>
    <name>A0A6I8VJK1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large proline-rich protein BAG6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">BCL2-associated athanogene 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">HLA-B-associated transcript 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26 27" type="primary">LOC4812896</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26 27">MV-25-SWS-2005</strain>
            <tissue evidence="26 27">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).</text>
    </comment>
    <comment type="function">
        <text evidence="1">Released extracellularly via exosomes, it is a ligand of the natural killer/NK cells receptor NCR3 and stimulates NK cells cytotoxicity. It may thereby trigger NK cells cytotoxicity against neighboring tumor cells and immature myeloid dendritic cells (DC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Component of the BAG6/BAT3 complex, also named BAT3 complex, at least composed of BAG6, UBL4A and GET4/TRC35. Interacts with GET4; the interaction is direct and localizes BAG6 in the cytosol. Interacts with UBL4A; the interaction is direct and required for UBL4A protein stability. Interacts with AIFM1. Interacts with HSPA2. Interacts with CTCFL. Interacts with p300/EP300. Interacts (via ubiquitin-like domain) with RNF126; required for BAG6-dependent ubiquitination of proteins mislocalized to the cytosol. Interacts (via ubiquitin-like domain) with SGTA; SGTA competes with RNF126 by binding the same region of BAG6, thereby promoting deubiquitination of BAG6-target proteins and rescuing them from degradation. Interacts with ricin A chain. Interacts with VCP and AMFR; both form the VCP/p97-AMFR/gp78 complex. Interacts with SYVN1. Interacts with USP13; the interaction is direct and may mediate UBL4A deubiquitination. Interacts with ZFAND2B. Interacts with KPNA2. Interacts with UBQLN4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015042655.2">
        <property type="nucleotide sequence ID" value="XM_015187169.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238211.1">
        <property type="nucleotide sequence ID" value="XM_033382320.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812896"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071818">
        <property type="term" value="C:BAT3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01809">
        <property type="entry name" value="Ubl_BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000161">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021925">
        <property type="entry name" value="BAG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204:SF0">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12057">
        <property type="entry name" value="BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="17" id="KW-0143">Chaperone</keyword>
    <keyword evidence="13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="24">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="281"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="516"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="590"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="698"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="793"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1115"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="608"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="707"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="805"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1121"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00046003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_015042655.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_033238211.1"/>
        </source>
    </evidence>
    <sequence length="1296" mass="138105" checksum="763F448511305278" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPJ2</accession>
    <name>A0A6I8VPJ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein PAT1 homolog 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Patr-1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033232956.1">
        <property type="nucleotide sequence ID" value="XM_033377065.1"/>
    </dbReference>
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        <property type="interactions" value="881"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VPJ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF0">
        <property type="entry name" value="PROTEIN ASSOCIATED WITH TOPO II RELATED-1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="471"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="589"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="589"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="612"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232956.1"/>
        </source>
    </evidence>
    <sequence length="997" mass="110326" checksum="1B8F6879FE2C89B7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQ65</accession>
    <name>A0A6I8VQ65_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Prosaposin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">Sap-r</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357588.3">
        <property type="nucleotide sequence ID" value="XM_001357551.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232874.1">
        <property type="nucleotide sequence ID" value="XM_033376983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQ65"/>
    <dbReference type="KEGG" id="dpo:4800324"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000002">
        <property type="entry name" value="prosaposin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000016">
        <property type="entry name" value="Saposin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000019">
        <property type="entry name" value="Saposin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003119">
        <property type="entry name" value="SAP_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008373">
        <property type="entry name" value="Saposin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF3">
        <property type="entry name" value="BCDNA.GH08312"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02199">
        <property type="entry name" value="SapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01797">
        <property type="entry name" value="SAPOSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00162">
        <property type="entry name" value="SAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00741">
        <property type="entry name" value="SapB"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51110">
        <property type="entry name" value="SAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50015">
        <property type="entry name" value="SAP_B"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634542" evidence="7">
        <location>
            <begin position="21"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin A-type" evidence="9">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="73"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="189"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="288"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="435"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="537"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="662"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="778"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin B-type" evidence="8">
        <location>
            <begin position="862"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357588.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033232874.1"/>
        </source>
    </evidence>
    <sequence length="957" mass="105838" checksum="80CBCC4A0E63C819" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VRP4</accession>
    <name>A0A6I8VRP4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial intermediate peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4804848</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233751.1">
        <property type="nucleotide sequence ID" value="XM_033377860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VRP4"/>
    <dbReference type="FunCoup" id="A0A6I8VRP4">
        <property type="interactions" value="1518"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804848"/>
    <dbReference type="InParanoid" id="A0A6I8VRP4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06457">
        <property type="entry name" value="M3A_MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000013">
        <property type="entry name" value="Mitochondrial intermediate peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033851">
        <property type="entry name" value="M3A_MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="11">
        <location>
            <begin position="239"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033233751.1"/>
        </source>
    </evidence>
    <sequence length="701" mass="80206" checksum="00479191DD1A307F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VRR7</accession>
    <name>A0A6I8VRR7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein GrlHz</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GrlHz</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233379.1">
        <property type="nucleotide sequence ID" value="XM_033377488.1"/>
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    <dbReference type="KEGG" id="dpo:4805555"/>
    <dbReference type="InParanoid" id="A0A6I8VRR7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077727">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38337">
        <property type="entry name" value="AGAP010540-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38337:SF1">
        <property type="entry name" value="GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233379.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="49896" checksum="AF5159BCB350ECF5" modified="2020-08-12" version="1">MTTPTSTPAATATEEELWSSRATSVASSWLHDELQPGEHEPTSALLDYCKRKFLRPYVTILTLVGLNPISTDMSNVRACCSYVQALMVLFILLMGYTLRYICGYRGDRGFTNYRDIRPINNSTDSGIAPPNTIGELLFGYVVPSAFNLLAFVSAVLVCKVIDHEQLQNLIERVFLMSAKPKRLCRMLWLYLGLALALLMLLFAYACAVVVMQPAQIIKVAWIADALSDWELCLRTGLLCTILLQDLVEIVILSNYYIECYLLKVHLESLSHKLLMHSIDSLDWMREVLEFRKLLDRLNQHVSVPVCFLIVMNLSYAFAGMVYLFKDFDFHFCALQLVLLNIANVMLWLFLGLLPFFVASSVTRVCQNAQANGHQIRVRPFVYHNTSAEDLNSTLLFASSLDMSAKLFRMPIQPNYLCFAILVVVIVILTLGMCLNLTALGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTA5</accession>
    <name>A0A6I8VTA5_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117183653</name>
        <name evidence="3" type="synonym">LOC117183652</name>
        <name evidence="5" type="synonym">LOC117183772</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3 4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4 5">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="3 4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234147.1">
        <property type="nucleotide sequence ID" value="XM_033378256.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033378257.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234923.1">
        <property type="nucleotide sequence ID" value="XM_033379032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTA5"/>
    <dbReference type="KEGG" id="dpo:117183652"/>
    <dbReference type="KEGG" id="dpo:117183653"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF6">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="88"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033234923.1"/>
        </source>
    </evidence>
    <sequence length="185" mass="20220" checksum="FD4927AE1EC6F989" modified="2020-08-12" version="1">MGGLWEAGVKSAKGLLLRAVGSALLTAEELETVLVGIEAVLNSRPLGPLSPDPSDGDALTPGHLLTGGPLIAPPAPRTPDQEGLSCLKRWRLVSSARQMFWQRWSREYVLGLQIRFKWHQEEPNIKEGDLVIVAEDNLPPQQWLLGRVVGTTAGQDGRVRVVELRTSSGATFRRPIHKLALLPMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VV20</accession>
    <name>A0A6I8VV20_DROPS</name>
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        <recommendedName>
            <fullName evidence="10">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">SerT</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15">MV-25-SWS-2005</strain>
            <strain evidence="13">MV2-25</strain>
            <tissue evidence="14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015040736.1">
        <property type="nucleotide sequence ID" value="XM_015185250.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234906.1">
        <property type="nucleotide sequence ID" value="XM_033379015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VV20"/>
    <dbReference type="SMR" id="A0A6I8VV20"/>
    <dbReference type="GeneID" id="6899063"/>
    <dbReference type="KEGG" id="dpo:6899063"/>
    <dbReference type="OMA" id="FWRACWS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246498">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005335">
        <property type="term" value="F:serotonin:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11497">
        <property type="entry name" value="SLC6sbd_SERT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF279">
        <property type="entry name" value="SODIUM-DEPENDENT SEROTONIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="5 10" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="463"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="576"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015040736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033234906.1"/>
        </source>
    </evidence>
    <sequence length="630" mass="70067" checksum="16B1D5B029D457A3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VW68</accession>
    <name>A0A6I8VW68_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein Snoo isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Snoo</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235327.1">
        <property type="nucleotide sequence ID" value="XM_033379436.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VW68">
        <property type="interactions" value="298"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VW68"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21079">
        <property type="entry name" value="DHD_Ski_Sno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000002">
        <property type="entry name" value="Putative ski oncogene"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.20:FF:000002">
        <property type="entry name" value="SKI-like oncogene a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.20">
        <property type="entry name" value="Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014890">
        <property type="entry name" value="c-SKI_SMAD4-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003380">
        <property type="entry name" value="SKI/SNO/DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037000">
        <property type="entry name" value="Ski_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023216">
        <property type="entry name" value="Tscrpt_reg_SKI_SnoN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005">
        <property type="entry name" value="SKI ONCOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005:SF25">
        <property type="entry name" value="SNO ONCOGENE, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08782">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02437">
        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01046">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="c-SKI SMAD4-binding" evidence="4">
        <location>
            <begin position="243"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="456"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="555"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="744"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="818"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="988"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="949"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="465"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="574"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="638"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="649"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="681"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="697"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="744"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="819"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="843"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="884"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="997"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1008"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1023"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1055"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1093"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1105"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1131"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1153"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1204"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1214"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1243"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1260"/>
            <end position="1278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235327.1"/>
        </source>
    </evidence>
    <sequence length="1329" mass="146408" checksum="32BB56487FDD75E2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWA8</accession>
    <name>A0A6I8VWA8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Eukaryotic translation initiation factor 4 gamma 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NAT1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with the serine/threonine protein kinases MKNK1 and MKNK2. Binds EIF4A and EIF3. Interacts with MIF4GD. Interacts with DAZAP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4G family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_033378973.1"/>
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        <property type="interactions" value="1581"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898803"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11559">
        <property type="entry name" value="W2_eIF4G1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 4 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000061">
        <property type="entry name" value="Eukaryotic translation initiation factor 4 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000064">
        <property type="entry name" value="Translation initiation factor 4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253:SF9">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 18" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MI" evidence="16">
        <location>
            <begin position="548"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="W2" evidence="15">
        <location>
            <begin position="723"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="248"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="382"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="534"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00040449"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00046720"/>
        </source>
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    <evidence type="ECO:0000256" key="14">
        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51366"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033234864.1"/>
        </source>
    </evidence>
    <sequence length="908" mass="103570" checksum="BB0250BCF2429EB1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWE3</accession>
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        <submittedName>
            <fullName evidence="12 13 14">Serine/threonine-protein kinase 10 isoform X1</fullName>
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        <name evidence="12 13 14" type="primary">Slik</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
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        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
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        </source>
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        <property type="nucleotide sequence ID" value="XM_033379009.1"/>
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        <property type="nucleotide sequence ID" value="XM_033379011.1"/>
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            <position position="66"/>
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            <name>ATP</name>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_033234900.1"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033234902.1"/>
        </source>
    </evidence>
    <sequence length="1663" mass="187832" checksum="ED186AE67C37ECB5" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8VZ52</accession>
    <name>A0A6I8VZ52_DROPS</name>
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        <recommendedName>
            <fullName evidence="3">Kynurenine formamidase</fullName>
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            <shortName evidence="3">KFase</shortName>
            <ecNumber evidence="3">3.5.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Arylformamidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">N-formylkynurenine formamidase</fullName>
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        </alternativeName>
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        <name evidence="6 7 8 9" type="primary">KFase</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7 8">MV-25-SWS-2005</strain>
            <tissue evidence="6 7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Required for elimination of toxic metabolites.</text>
    </comment>
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        <reaction evidence="3">
            <text>N-formyl-L-kynurenine + H2O = L-kynurenine + formate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
            <dbReference type="EC" id="3.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the kynurenine formamidase family.</text>
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        <property type="nucleotide sequence ID" value="XM_033380494.1"/>
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        <property type="nucleotide sequence ID" value="XM_033380495.1"/>
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        <property type="nucleotide sequence ID" value="XM_033380496.1"/>
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        <property type="reaction ID" value="UER00454"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="baseline"/>
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    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KFase"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
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        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
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        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027519">
        <property type="entry name" value="KFase_ver/fungi-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF33">
        <property type="entry name" value="KYNURENINE FORMAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="domain" description="BD-FAE-like" evidence="4">
        <location>
            <begin position="76"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HGGXW" evidence="3">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033236384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033236385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033236386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033236387.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="35239" checksum="41C0B49EA9706CF8" modified="2020-08-12" version="1">MYNPKSTDLERDYFPSYHTIRFQDQPAPNLAVQLHFLQLTKQHADTIPEGKDLTTKQGIKVDHLRYGGQDDRQIVDVFYNDSTTDSSPLFVYVHGGYWQFLDITESCSMVGPLVRRGYRVAVMDYNLCPQVTLEQLMLEFTNFLNWTFAYAEKIKTTEIHFAGHSAGGHMLAQILNAPEVVTPERRKNIRSLIFICGVYDLRELWSLENVNPNNILGLNAEKAAALSPILWKYPDAKSWSSIKFHVMAAEHESLTFIEQSRVFGQVLEKAGFPTSFKVIQGYDHFDIIEETAIDDSIIAKNLQEILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3K8</accession>
    <name>A0A6I8W3K8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein argonaute-2 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC26531987</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237930.1">
        <property type="nucleotide sequence ID" value="XM_033382039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W3K8"/>
    <dbReference type="FunCoup" id="A0A6I8W3K8">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W3K8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="312"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="585"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033237930.1"/>
        </source>
    </evidence>
    <sequence length="913" mass="104130" checksum="16638A05BE3FE6E0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3X7</accession>
    <name>A0A6I8W3X7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6899908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237399.1">
        <property type="nucleotide sequence ID" value="XM_033381508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W3X7"/>
    <dbReference type="KEGG" id="dpo:6899908"/>
    <dbReference type="InParanoid" id="A0A6I8W3X7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026357683" description="MD-2-related lipid-recognition domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033237399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19467" checksum="30E617FF5621DAFF" modified="2020-08-12" version="1">MLKLFTITTLLVGTQATGKCTFNEKHFNNFTLAIVNNTLYMDLVTPRTIPRGLKVFMDVQISLDKRRNYQRLFAYVLDVCAVVSSVKSSMFKSWFQSILQHGNFMSNCPVPPGRYFLHDWKLDSQLVPHYLIPGDYRVFSHFFYGKHKTRHEDFVLDIDIYAVMRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7M1</accession>
    <name>A0A6I8W7M1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Uncharacterized protein Ank2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">Ank2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15">MV-25-SWS-2005</strain>
            <tissue evidence="14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033239375.1">
        <property type="nucleotide sequence ID" value="XM_033383484.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239376.1">
        <property type="nucleotide sequence ID" value="XM_033383485.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900405"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W7M1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08317">
        <property type="entry name" value="Death_ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000104">
        <property type="entry name" value="Ankyrin 2, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000009">
        <property type="entry name" value="Ankyrin 2, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000095">
        <property type="entry name" value="Ankyrin 2, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000001">
        <property type="entry name" value="Ankyrin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000001">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2660:FF:000001">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040745">
        <property type="entry name" value="Ankyrin_UPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033239375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033239376.1"/>
        </source>
    </evidence>
    <sequence length="15258" mass="1667529" checksum="7EEDEA7EDA13883F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W863</accession>
    <name>A0A6I8W863_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239472.1">
        <property type="nucleotide sequence ID" value="XM_033383581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W863"/>
    <dbReference type="KEGG" id="dpo:117184758"/>
    <dbReference type="InParanoid" id="A0A6I8W863"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31511">
        <property type="entry name" value="PROTEIN CBG23764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31511:SF12">
        <property type="entry name" value="RHO TERMINATION FACTOR N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="64116" checksum="0FB2949361467A36" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9E1</accession>
    <name>A0A6I8W9E1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proteasome inhibitor PI31 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240008.1">
        <property type="nucleotide sequence ID" value="XM_033384117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9E1"/>
    <dbReference type="KEGG" id="dpo:117184924"/>
    <dbReference type="InParanoid" id="A0A6I8W9E1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021625">
        <property type="entry name" value="PI31_Prot_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11566">
        <property type="entry name" value="PI31_Prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI31 proteasome regulator N-terminal" evidence="1">
        <location>
            <begin position="53"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240008.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="26007" checksum="B1114DF2B4DC26F3" modified="2020-08-12" version="1">MNDTDDNGPHAALPSSFVSLRSVGTTTFEAKTVSASEDGQFYGWQLLFYSIRRSIRKKADLLLALAHFLVTKQYHLRSVDPEHGQGIRMSAGSRLIPVDPGRGGSELLPMHWNRESSKYRLHYVDELDHQYVLLARLTHQDLVINLQNWTSKRMSITCLQPEDLVQTLHNSDLGQCIPTAHQVMHHLRVYLVNPVVRGRRKGLPESRPDSLKGNYNLCHSVTALGSEKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9Q6</accession>
    <name>A0A6I8W9Q6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP-binding protein REM 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6901796</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. RGK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240123.1">
        <property type="nucleotide sequence ID" value="XM_033384232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9Q6"/>
    <dbReference type="FunCoup" id="A0A6I8W9Q6">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901796"/>
    <dbReference type="InParanoid" id="A0A6I8W9Q6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000664">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051641">
        <property type="entry name" value="RGK_GTP-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775">
        <property type="entry name" value="RAD, GEM/KIR FAMILY MEMBER 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775:SF6">
        <property type="entry name" value="RAD, GEM_KIR FAMILY MEMBER 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="46"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="149"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="608"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="634"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033240123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1105" mass="121862" checksum="5883C8E58365C0EB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WB28</accession>
    <name>A0A6I8WB28_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6899967</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240598.1">
        <property type="nucleotide sequence ID" value="XM_033384707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WB28"/>
    <dbReference type="KEGG" id="dpo:6899967"/>
    <dbReference type="InParanoid" id="A0A6I8WB28"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033240598.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="51278" checksum="036340A3AFF65392" modified="2020-08-12" version="1">MTFFTQCRVVSATTYKRILPDESQAHCEMERLRELTQRIRPSDVDEGRIGSIELNVWLAQLTGLPLSGLKPETKAESIRILVVSGVVLPLLFCYVVLEIYDLVLNWDNVDIMTQNVVMTLTHVGYWFKVLNTFYYYEDIRRIVFTLKHLTRTCVLSPGQRETFHQVEVENKVVCLFYFCLVVFSSTLAMVMLLIVPDNLAGKRFPYRVHMPHFLPPIVQYLYMGLSIIWISCGIPTIDNVNMLFMNQICMHLKILNMAFDVLQRQVDPNIWMVSIVKYHSVLINLRQRLEQIYRLPVLFQFVSSLLVVAMTAFQAIVGDGSGSSVLIYFLFGGVMCQIFLYCWFGNEVFEQVHSHDTHIYSFRWVYSSYFQSKTLSTSAFGCNWHEFDAQFKRTLLIFMINADRPFLFTAGGFMGLTLTSFANILGKSYSIVTVLRHMYGRAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCI9</accession>
    <name>A0A6I8WCI9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translation initiation factor IF-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6899330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240967.1">
        <property type="nucleotide sequence ID" value="XM_033385076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCI9"/>
    <dbReference type="KEGG" id="dpo:6899330"/>
    <dbReference type="InParanoid" id="A0A6I8WCI9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240967.1"/>
        </source>
    </evidence>
    <sequence length="247" mass="27488" checksum="EF86D3D9C87E46C0" modified="2020-08-12" version="1">MQHHLIKYLTEQRKWIRRGYPAIGDGQRASRSRSPELQLLASPLVSPAGSTGPTMSPVVSTGYTDEGSDLELVSWEPAPRRKRAHEAKREEPPTNRREEPRNSRRKEPSASKREEPRAEEREEHRASRRKGPRVSKREEPHTVNREESRTSKREERPERGQRGHTTRKEEPRERVSKRASTQSTRAPREEASSARTRGSGGAEATAGVGGRPGAGEEAQGRAGGREGGARSGGGAPPTRRRAPMEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCT6</accession>
    <name>A0A6I8WCT6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein KIAA0100</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hob</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241057.1">
        <property type="nucleotide sequence ID" value="XM_033385166.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8WCT6">
        <property type="interactions" value="1690"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900510"/>
    <dbReference type="InParanoid" id="A0A6I8WCT6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="3">
        <location>
            <begin position="1074"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2121"/>
            <end position="2150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1885"/>
            <end position="1946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="616"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2123"/>
            <end position="2145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033241057.1"/>
        </source>
    </evidence>
    <sequence length="2302" mass="263518" checksum="67110F76C1991215" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WD98</accession>
    <name>A0A6I8WD98_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein max</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myc-associated factor X</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Max</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241391.1">
        <property type="nucleotide sequence ID" value="XM_033385500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WD98"/>
    <dbReference type="FunCoup" id="A0A6I8WD98">
        <property type="interactions" value="1899"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WD98"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WD98">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11406">
        <property type="entry name" value="bHLHzip_Max"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000023">
        <property type="entry name" value="MAX isoform 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328:SF3">
        <property type="entry name" value="PROTEIN MAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328">
        <property type="entry name" value="PROTEIN MAX MYC-ASSOCIATED FACTOR X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="39"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033241391.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="19166" checksum="C9B51F92AB48FAEC" modified="2020-08-12" version="1">MSMSDDDRDIDIESDDDGDSDTGLGSSRHTNTANFTQAEKRAHHNALERRRRDHIKESFTNLREAVPTLKGEKTFPQASRAQILKRTTECIQTMRKKINENQKDIDEIKKQNILLDQQIRAMEGGMNGDVYPDLLSEDDVCSDDVEDEDLELPDSSRRNKKMKTFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>B5DHF4</accession>
    <accession>A0A6I8UX26</accession>
    <name>B5DHF4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex I-B22</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase B22 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ND-B22</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132328.1">
        <property type="nucleotide sequence ID" value="XM_002132292.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DHF4"/>
    <dbReference type="SMR" id="B5DHF4"/>
    <dbReference type="FunCoup" id="B5DHF4">
        <property type="interactions" value="1393"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DHF4"/>
    <dbReference type="GeneID" id="6903449"/>
    <dbReference type="KEGG" id="dpo:6903449"/>
    <dbReference type="eggNOG" id="KOG3466">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108081_0_1_1"/>
    <dbReference type="InParanoid" id="B5DHF4"/>
    <dbReference type="OMA" id="YRAVQMR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246785">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DHF4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20263">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045292">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033034">
        <property type="entry name" value="NDUFB9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B22 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002132328.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="17327" checksum="A7DA2497D1E00813" modified="2008-10-14" version="1">MAQVPLAIASHKRQVCSLYKRALRNLEAWYDRRHDYRYRAVQMRARFDENRCKDLGEGIRLLACGQKELFETKHFQPKTFANSAGGCAFEREVIPPDWLLDYWHPLEKAQFPEYFAKREQRKQQYVVWWQKQYGKPDPKDLGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>B5DP05</accession>
    <accession>A0A6I8VJ21</accession>
    <name>B5DP05_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">Ypel</name>
        <name evidence="7" type="synonym">LOC6901004</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134630.1">
        <property type="nucleotide sequence ID" value="XM_002134594.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042379.1">
        <property type="nucleotide sequence ID" value="XM_015186893.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DP05"/>
    <dbReference type="SMR" id="B5DP05"/>
    <dbReference type="STRING" id="46245.B5DP05"/>
    <dbReference type="GeneID" id="6901004"/>
    <dbReference type="KEGG" id="dpo:6901004"/>
    <dbReference type="eggNOG" id="KOG3399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043857_3_2_1"/>
    <dbReference type="OMA" id="SSRIYGC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249283">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="14"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002134630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015042379.1"/>
        </source>
    </evidence>
    <sequence length="114" mass="12923" checksum="D4F650313385C06D" modified="2008-10-14" version="1">MVKTFQAYLPSTNRTYSCVHCRAHLASHDELISKSFQGSQGPAYLFNSVVNVACGQTEERVLLTGLHAVADIYCECCKTPLGWKYEHAYESSQKYKEGKFIIELAHMIKENGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPX7</accession>
    <accession>A0A6I8V0S8</accession>
    <name>B5DPX7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Larval cuticle protein 65Ag1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135073.3">
        <property type="nucleotide sequence ID" value="XM_002135037.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPX7"/>
    <dbReference type="STRING" id="46245.B5DPX7"/>
    <dbReference type="KEGG" id="dpo:6900710"/>
    <dbReference type="KEGG" id="dpo:6900711"/>
    <dbReference type="eggNOG" id="ENOG502ST8A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_7_3_1"/>
    <dbReference type="InParanoid" id="B5DPX7"/>
    <dbReference type="OMA" id="LVIFEDF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF218">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 65AA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0193">Cuticle</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135073.3"/>
        </source>
    </evidence>
    <sequence length="101" mass="11013" checksum="53BC23CD43165B30" modified="2008-10-14" version="1">MKFVIVLVALFALAAARPNRDAETLRYDSNVEPENYNYNVQTSDGKSVAEEGHVEDLGTEQEAIVVKGSYSYIGDDGVTYSVNYIADKNGFQPQGAHIPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>B5DR47</accession>
    <accession>A0A6I8V117</accession>
    <name>B5DR47_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Importin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Arts</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002135399.1">
        <property type="nucleotide sequence ID" value="XM_002135363.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DR47"/>
    <dbReference type="SMR" id="B5DR47"/>
    <dbReference type="FunCoup" id="B5DR47">
        <property type="interactions" value="1653"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DR47"/>
    <dbReference type="GeneID" id="6900034"/>
    <dbReference type="KEGG" id="dpo:6900034"/>
    <dbReference type="eggNOG" id="KOG2171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003794_1_2_1"/>
    <dbReference type="InParanoid" id="B5DR47"/>
    <dbReference type="OMA" id="ANACGCV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249883">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DR47">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002135399.1"/>
        </source>
    </evidence>
    <sequence length="1079" mass="120922" checksum="FB26152F7F3EC589" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>B5DRI4</accession>
    <accession>A0A6I8V180</accession>
    <name>B5DRI4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6900212</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135544.1">
        <property type="nucleotide sequence ID" value="XM_002135508.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DRI4"/>
    <dbReference type="STRING" id="46245.B5DRI4"/>
    <dbReference type="GeneID" id="6900212"/>
    <dbReference type="KEGG" id="dpo:6900212"/>
    <dbReference type="eggNOG" id="ENOG502TG4F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062693_1_0_1"/>
    <dbReference type="InParanoid" id="B5DRI4"/>
    <dbReference type="OMA" id="RTCTRYV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249973">
        <property type="expression patterns" value="Expressed in multicellular organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DRI4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="47"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="103"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="159"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="238"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002135544.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="32891" checksum="4ABD45BD9C7403BF" modified="2008-10-14" version="1">MTGAKRLSILGLLLCSHFLVKAVDADDLQAREDDGAPASNDLILGNLSLCENVADSVFLPYVGDCNKYYLCRYGEAIELQCEWPYLFSAPTQSCVNSTQAHCLPTCSNFQLTTFSYERTCTKYVLCYYGHPVLRECLDGLQYNAQTDRCDFPQNVDCVESECSIYYNAYQLHYVPSKVSCEKYFLCGNGVPREQTCTPGLYFSTKCNCCVLPSNSDCQIPSRKKIVQPFSRLSPRIADIMCPSAGVHFYSHESRKDAYYFCVDGHGLTLDCSPGLWYDGKEQECREPKNVEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-03" modified="2025-06-18" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q294B9</accession>
    <name>SUV39_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Histone-lysine N-methyltransferase Su(var)3-9</fullName>
            <ecNumber evidence="2">2.1.1.355</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Histone H3-K9 methyltransferase</fullName>
            <shortName>H3-K9-HMTase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein suppressor of variegation 3-9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">Su(var)3-9</name>
        <name type="ORF">GA19622</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting Su(var)205/HP1 to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric regions. Involved in heterochromatic gene silencing including the modification of position-effect-variegation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-lysyl(9)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60276"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15546"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.355"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with Su(var)205 and Su(var)3-7. Probably associates with HDAC1/Rpd3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
        <text evidence="1">Associates with centromeric constitutive heterochromatin.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates in stable binding to heterochromatin.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.355" evidence="2"/>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL29045.3"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q294B9"/>
    <dbReference type="SMR" id="Q294B9"/>
    <dbReference type="FunCoup" id="Q294B9">
        <property type="interactions" value="1602"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q294B9"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0310181">
        <property type="protein sequence ID" value="FBpp0301866"/>
        <property type="gene ID" value="FBgn0079618"/>
    </dbReference>
    <dbReference type="GeneID" id="4803095"/>
    <dbReference type="KEGG" id="dpo:4803095"/>
    <dbReference type="eggNOG" id="KOG1082">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020840_8_1_1"/>
    <dbReference type="InParanoid" id="Q294B9"/>
    <dbReference type="PhylomeDB" id="Q294B9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079618">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q294B9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140949">
        <property type="term" value="F:histone H3K9 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10542">
        <property type="entry name" value="SET_SUV39H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000051">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002336">
        <property type="entry name" value="Histone-lysine N-methyltransferase Su(var)3-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011381">
        <property type="entry name" value="H3-K9_MeTrfase_SUV39H1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050973">
        <property type="entry name" value="H3K9_Histone-Lys_N-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SUV39H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
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    <keyword id="KW-0156">Chromatin regulator</keyword>
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    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
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    <keyword id="KW-0805">Transcription regulation</keyword>
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    <keyword id="KW-0862">Zinc</keyword>
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    <feature type="binding site" evidence="1">
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            <name>Zn(2+)</name>
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            <name>S-adenosyl-L-methionine</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
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        <source>
            <dbReference type="UniProtKB" id="P45975"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00053"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00190"/>
        </source>
    </evidence>
    <sequence length="633" mass="71612" checksum="094CDAAA9499BD32" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-23" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q297L2</accession>
    <name>NRBP_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Nuclear receptor-binding protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>MLF1-adaptor molecule</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Madm</name>
        <name type="ORF">GA10685</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The protein kinase domain is predicted to be catalytically inactive.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL28193.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="FBpp0284176"/>
        <property type="gene ID" value="FBgn0070741"/>
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    <dbReference type="GeneID" id="4802057"/>
    <dbReference type="KEGG" id="dpo:4802057"/>
    <dbReference type="CTD" id="40710"/>
    <dbReference type="eggNOG" id="KOG1266">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_024273_0_0_1"/>
    <dbReference type="InParanoid" id="Q297L2"/>
    <dbReference type="OMA" id="NEVQYAQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070741">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd13984">
        <property type="entry name" value="PK_NRBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000351197" description="Nuclear receptor-binding protein homolog">
        <location>
            <begin position="1"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="122"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="481"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="638"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="641"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="3">
        <location>
            <position position="489"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="3">
        <location>
            <position position="495"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="3">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine" evidence="3">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9UHY1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q9Y0Y6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="663" mass="73520" checksum="8D5F50FEEA65D978" modified="2008-09-23" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CV2</accession>
    <name>MED26_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Mediator of RNA polymerase II transcription subunit 26</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Mediator complex subunit 26</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">MED26</name>
        <name type="ORF">GA14731</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Mediator complex subunit 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH475401">
        <property type="protein sequence ID" value="EAL29261.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001352375.2">
        <property type="nucleotide sequence ID" value="XM_001352339.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29CV2"/>
    <dbReference type="SMR" id="Q29CV2"/>
    <dbReference type="FunCoup" id="Q29CV2">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29CV2"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0273954">
        <property type="protein sequence ID" value="FBpp0272392"/>
        <property type="gene ID" value="FBgn0074758"/>
    </dbReference>
    <dbReference type="GeneID" id="4811809"/>
    <dbReference type="KEGG" id="dpo:4811809"/>
    <dbReference type="CTD" id="9441"/>
    <dbReference type="eggNOG" id="ENOG502S9CY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_249548_0_0_1"/>
    <dbReference type="InParanoid" id="Q29CV2"/>
    <dbReference type="OMA" id="NALHNFY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074758">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29CV2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042376">
        <property type="entry name" value="MED26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15201">
        <property type="entry name" value="CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15201:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000304963" description="Mediator of RNA polymerase II transcription subunit 26">
        <location>
            <begin position="1"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="238"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="399"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="580"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="694"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1035"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1372"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="792"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="401"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="587"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="715"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1056"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1073"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1101"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1124"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1144"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1378"/>
            <end position="1388"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="1602" mass="178182" checksum="ADD5BBC9394DC725" modified="2009-01-20" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q29ET2</accession>
    <accession>A0A6I8UAK8</accession>
    <name>Q29ET2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Gtpx</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352481.1">
        <property type="nucleotide sequence ID" value="XM_001352445.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29ET2"/>
    <dbReference type="SMR" id="Q29ET2"/>
    <dbReference type="FunCoup" id="Q29ET2">
        <property type="interactions" value="1398"/>
    </dbReference>
    <dbReference type="PeroxiBase" id="5644">
        <property type="entry name" value="DpGPx02"/>
    </dbReference>
    <dbReference type="GeneID" id="4812067"/>
    <dbReference type="KEGG" id="dpo:4812067"/>
    <dbReference type="eggNOG" id="KOG1651">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029507_0_0_1"/>
    <dbReference type="InParanoid" id="Q29ET2"/>
    <dbReference type="OMA" id="TFPMTEK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071390">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000025">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 4 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352481.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="26271" checksum="B27C89E2C101E144" modified="2006-04-04" version="1">MSLRQFQTISQQVFRCLSTRAATSRRILGPVIELSQSQRQNLKFCTVLLPVSCAAAVSAPQYSTAAAIDMSANGDYKNAASIYEFTVKDTHGSEVSLDKYKGRVLLVVNIASKCGLTKNNYQKLTELKEKFGERGLTILNFPCNQFNSQMPEADGEAMVCHLRDAKADIGEIFARVDVNGDNAAPVYKYLKAKQSGTLGSGLKWNFTKFLVNKEGIPINRYAPTTDPMDIAKDIEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HB6</accession>
    <accession>A0A6I8UFS9</accession>
    <name>Q29HB6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cytochrome P450 4g1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Cyp4g1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354787.1">
        <property type="nucleotide sequence ID" value="XM_001354751.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HB6"/>
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        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HB6"/>
    <dbReference type="GeneID" id="4814429"/>
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    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_1_1"/>
    <dbReference type="InParanoid" id="Q29HB6"/>
    <dbReference type="OMA" id="WRRNSRE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077822">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HB6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20628">
        <property type="entry name" value="CYP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000035">
        <property type="entry name" value="CYtochrome P450 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450"/>
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        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_sf"/>
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    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
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        <property type="entry name" value="CYTOCHROME P450 4G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000461"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001354787.1"/>
        </source>
    </evidence>
    <sequence length="552" mass="62665" checksum="AB5D1C2AEDD6260D" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IH4</accession>
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        <submittedName>
            <fullName evidence="6">General odorant-binding protein 19d</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Obp19d</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="6">MV-25-SWS-2005</strain>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:odorant binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
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        <property type="entry name" value="PBP/GOBP"/>
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        <property type="entry name" value="PBP_GOBP_sf"/>
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        <property type="entry name" value="GENERAL ODORANT-BINDING PROTEIN 19D-RELATED"/>
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    </dbReference>
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    </dbReference>
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    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043354726" evidence="4">
        <location>
            <begin position="22"/>
            <end position="151"/>
        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001355620.2"/>
        </source>
    </evidence>
    <sequence length="151" mass="16767" checksum="6061B893B4840518" modified="2008-10-14" version="2">MSRLVCLGLMLAVCLVAVSESESKPQDASNEEHVAEAANECKAETGATDEDVELMMKHEPSVNEEGKCLRACMMKKFKIIEEDGKPNTEHTIEMMKLMSENAEEKEDAITEIADTCAAIDLPEDHCDSADGYLKCIIEHMEKHGITLRVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q29J10</accession>
    <accession>A0A6I8UH37</accession>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein pck</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pck</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355434.2">
        <property type="nucleotide sequence ID" value="XM_001355398.4"/>
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    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073275">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29J10">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of tube size, open tracheal system"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019991">
        <property type="term" value="P:septate junction assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000053">
        <property type="entry name" value="EG:80H7.2 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21284">
        <property type="entry name" value="EG:80H7.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21284:SF12">
        <property type="entry name" value="EG:80H7.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001355434.2"/>
        </source>
    </evidence>
    <sequence length="256" mass="29755" checksum="914DD37612D88A26" modified="2008-10-14" version="2">MRELNKQQSQDTTDGGIEKGDYPRATNGVVFGAILTFGSSFVLMMSFCSPYWIESYEETRSSFKNMGLWQYCFKDFVYPKYAFPKQFTGCHNIFSHEYYVIREYLLPGWLMAVQGFVTMSFIIVFLVLTLLALTIIRLPLKPVLQYEWLLVRLSYMGTAISSLFMFLAVCIFGGCAYRRDWLMYPRFNVLGWSYAMAVVTFMLLGLAALILHREARQAFEARGEQKNLVMQMEMQEPGYQPPRHHHSQSRSLQGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KP2</accession>
    <accession>A0A6I8UIJ0</accession>
    <name>Q29KP2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Voltage-dependent anion-selective channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">porin</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356072.1">
        <property type="nucleotide sequence ID" value="XM_001356036.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KP2"/>
    <dbReference type="SMR" id="Q29KP2"/>
    <dbReference type="FunCoup" id="Q29KP2">
        <property type="interactions" value="1861"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KP2"/>
    <dbReference type="GeneID" id="4816719"/>
    <dbReference type="KEGG" id="dpo:4816719"/>
    <dbReference type="eggNOG" id="KOG3126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044399_2_0_1"/>
    <dbReference type="InParanoid" id="Q29KP2"/>
    <dbReference type="OMA" id="MAPPCYA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079746">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KP2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000001">
        <property type="entry name" value="Voltage-dependent anion-selective channel protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF70">
        <property type="entry name" value="GH26960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00185">
        <property type="entry name" value="EUKARYTPORIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0626">Porin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356072.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="30578" checksum="A405D6ED0992905B" modified="2006-04-04" version="1">MAPPSYSDLGKQARDIFSKGYNFGLWKLDLKTKTPSGIEFNTAGHSNQESGKVFGSLETKYKVKDYGLTLTEKWNTDNTLFTEVAVQDQLLEGLKLSLEGNFAPQSGNKNGKFKVGFGHEYVKADSDVNIDLKGPLINASAVLGYNGWLAGYQAAFDTQQTKLTTNNFALGYTTKDFVLHTSVNDGQEFSGSIFQRTSDKLDVGVQLSWASGNSNTKFAIGAKYQLGDDASVRAKVNNASQVGLGYQQKLRDGITLTLSTLVDGKNFNDGGHKIGVGLELEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MA5</accession>
    <name>RM24_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein uL24m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">39S ribosomal protein L24, mitochondrial</fullName>
            <shortName>L24mt</shortName>
            <shortName>MRP-L24</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mRpL24</name>
        <name type="ORF">GA21365</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379060">
        <property type="protein sequence ID" value="EAL33789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001356724.1">
        <property type="nucleotide sequence ID" value="XM_001356688.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MA5"/>
    <dbReference type="SMR" id="Q29MA5"/>
    <dbReference type="FunCoup" id="Q29MA5">
        <property type="interactions" value="669"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MA5"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0282179">
        <property type="protein sequence ID" value="FBpp0280617"/>
        <property type="gene ID" value="FBgn0081353"/>
    </dbReference>
    <dbReference type="GeneID" id="4817158"/>
    <dbReference type="KEGG" id="dpo:4817158"/>
    <dbReference type="CTD" id="79590"/>
    <dbReference type="eggNOG" id="KOG1708">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093315_0_1_1"/>
    <dbReference type="InParanoid" id="Q29MA5"/>
    <dbReference type="OMA" id="DFEWRFT"/>
    <dbReference type="PhylomeDB" id="Q29MA5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081353">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000032">
        <property type="entry name" value="39S ribosomal protein L24, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_uL24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_uL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057264">
        <property type="entry name" value="Ribosomal_uL24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end status="unknown"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000270496" description="Large ribosomal subunit protein uL24m">
        <location>
            <begin status="unknown"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="KOW">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q96A35"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="247" mass="29343" checksum="BC1C4E4374583527" modified="2006-04-04" version="1" precursor="true">MRITQFLASKLKNFSNLPKEYIERSKKQVYWQTPREKNYLPRTVERKRFRYTTNRPWTGQFRQQNMPATMRRKVLIEPVEDWSFFRGDRIEVLVGKDKGKQGIVTQVIPERNWVIVEGLNWHYRRVGAEKEFPGIIIKSEAPLHVLNDIRLVDPSDLLGTEFEWRFTEEGEKVRVSMRSGRIIPIPETNNQTHDYKTPNAYIEREKDTPAAVVGEITFQPKLSTFEMDIMEEMGIKEERTPAKSYWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0E6</accession>
    <accession>A0A6I8UD21</accession>
    <name>Q2M0E6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353477.1">
        <property type="nucleotide sequence ID" value="XM_001353441.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0E6"/>
    <dbReference type="STRING" id="46245.Q2M0E6"/>
    <dbReference type="GeneID" id="4813705"/>
    <dbReference type="KEGG" id="dpo:4813705"/>
    <dbReference type="eggNOG" id="KOG2078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2123667_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M0E6"/>
    <dbReference type="OMA" id="NFQSLHA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076820">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0E6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353477.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="12211" checksum="66C4C15AF3EA900D" modified="2006-02-21" version="1">MGRNKANNKKVAPAAKPKTPLNKSKKAKNLFKVADNRKGKGKKPKEVQGNLKQIKESVKAKQEKVDATLKTLHKDMVVKKPKPAVFPIKNKRKPAANTEKVSDTLGKLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0W5</accession>
    <accession>A0A6I8VKA6</accession>
    <name>Q2M0W5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Neurogenic protein mastermind isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">LOC4813463</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353308.2">
        <property type="nucleotide sequence ID" value="XM_001353272.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042730.1">
        <property type="nucleotide sequence ID" value="XM_015187244.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241065.1">
        <property type="nucleotide sequence ID" value="XM_033385174.1"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0W5"/>
    <dbReference type="GeneID" id="4813463"/>
    <dbReference type="KEGG" id="dpo:4813463"/>
    <dbReference type="eggNOG" id="KOG4805">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004761_1_0_1"/>
    <dbReference type="OMA" id="MIMSPAA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0W5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025451">
        <property type="entry name" value="DUF4211"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14689:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14689">
        <property type="entry name" value="PHORBOL-ESTER_DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13926">
        <property type="entry name" value="DUF4211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4211" evidence="3">
        <location>
            <begin position="1263"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="510"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="603"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="752"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="853"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="965"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1043"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="689"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="532"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="603"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="759"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="803"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="972"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1053"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1080"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1100"/>
            <end position="1138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353308.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015042730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033241065.1"/>
        </source>
    </evidence>
    <sequence length="1486" mass="164344" checksum="AE41F845AE51248A" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P0L7</accession>
    <accession>A0A6I8W8G8</accession>
    <name>A0A0R3P0L7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribonuclease P protein subunit p20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Rpp20</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of nuclear RNase P and RNase MRP complexes. RNase P consists of a catalytic RNA moiety and 10 different protein chains; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5. Interacts with SMN1. POP7 forms a heterodimer with RPP25 that binds to the P3 stem loop of the catalytic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239670.1">
        <property type="nucleotide sequence ID" value="XM_033383779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P0L7"/>
    <dbReference type="FunCoup" id="A0A0R3P0L7">
        <property type="interactions" value="928"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P0L7"/>
    <dbReference type="KEGG" id="dpo:26533210"/>
    <dbReference type="eggNOG" id="KOG3631">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3P0L7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272197">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P0L7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000002">
        <property type="entry name" value="Ribonuclease P protein subunit p20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014612">
        <property type="entry name" value="Pop7/Rpp20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15314">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15314:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12328">
        <property type="entry name" value="Rpp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="156"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033239670.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="22089" checksum="42EFC61B7F3B1546" modified="2016-01-20" version="1">MMESNAPELGAKPRTNNRNSHSRRNQSDSANHRVVRKLPPRADNDRCNIYITSKTDFKAQQRRCEELLNSGVKEIFLHCMGYSITRGLNLALRLIKNSEGALGYTINTSTIQLVDELHPLCDEDDITFRQRNNSALHIKIFSNNLFNIDVPRLPAQPALPKVSQREPPKPHQQQQQQQPQKAKAKKQTTRSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P194</accession>
    <accession>A0A6I8VMA5</accession>
    <name>A0A0R3P194_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">dTCF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pan</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Segment polarity protein. Functions together with arm to transduce the Wingless (Wg) signal in embryos and in developing adult tissues. Acts as a transcriptional activator, but in the absence of arm, it binds to gro and acts as a transcriptional repressor of wg-responsive genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Binds to the beta-catenin homolog arm or to gro.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
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        <property type="nucleotide sequence ID" value="XM_015188834.2"/>
    </dbReference>
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        <property type="interactions" value="139"/>
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    <dbReference type="STRING" id="46245.A0A0R3P194"/>
    <dbReference type="GeneID" id="4811795"/>
    <dbReference type="InParanoid" id="A0A0R3P194"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074773">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P194">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001222">
        <property type="term" value="F:transcription corepressor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:imaginal disc-derived wing morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:mesodermal cell fate determination"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of stem cell proliferation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:segment polarity determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:spiracle morphogenesis, open tracheal system"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="transcription factor 7 isoform X2"/>
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        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HMG_box_dom"/>
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        <property type="entry name" value="HMG_box_dom_sf"/>
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        <property type="entry name" value="PROTEIN PANGOLIN, ISOFORM J"/>
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        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
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        <property type="entry name" value="HMG_box"/>
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        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="857"/>
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        <location>
            <begin position="1018"/>
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            <begin position="1030"/>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="RefSeq" id="XP_015044320.1"/>
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    <sequence length="1364" mass="148995" checksum="D35AB9E3E1AD0528" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P4Z1</accession>
    <accession>A0A6I8W6B3</accession>
    <name>A0A0R3P4Z1_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Zwei Ig domain protein zig-8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dpr8</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
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        </source>
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        <property type="entry name" value="Uncharacterized protein, isoform A"/>
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        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 8, ISOFORM A"/>
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        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
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        <property type="entry name" value="ig"/>
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        <property type="entry name" value="IGc2"/>
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        <location>
            <begin position="1"/>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033238837.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="36902" checksum="3FCA98FCCF00704F" modified="2016-01-20" version="1">MSMNWALYWGIMWVLSGSTDSASKRFFTDFLQDLPTPGTGGPTFDPSAISNITGLVGKTVKMTCRVKNLGNRTVSWVRHRDIHLLTVGRYTYTSDQRFEAQHSPHAEDWSLRIRYAQRKDSGIYECQISTTPPIGHAVYLNIVEPITEVIGGPDLHINKGSTINLTCIVKFAPEPPPTVSWSHNRENDPRYNKVINFDSPRGGISLVTEKGVLTTSRLLVQKAIQEDEGLYTCAPSNANPTSVRVHIVDGEHPAAMHHGNSAPLQPPALISMLMLVCSTLILLHASACCSPTLPKMRTQQQQQQQQQRGGGNGNGQLKDIKGGERLERNSLVPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCE3</accession>
    <name>A0A6I8UCE3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Trypsin beta-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4813051</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Selective cleavage of 103-Arg-|-Ser-104 and 124-Ile-|-Ile-125 bonds in Limulus clotting factor B to form activated factor B. Cleavage of -Pro-Arg-|-Xaa- bonds in synthetic substrates.</text>
            <dbReference type="EC" id="3.4.21.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353252.3">
        <property type="nucleotide sequence ID" value="XM_001353216.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCE3"/>
    <dbReference type="KEGG" id="dpo:4813051"/>
    <dbReference type="InParanoid" id="A0A6I8UCE3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042381">
        <property type="term" value="P:hemolymph coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000120">
        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF97">
        <property type="entry name" value="GH13245P2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0222">Digestion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0353">Hemolymph clotting</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0768">Sushi</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026211834" evidence="13">
        <location>
            <begin position="27"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="97"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001353252.3"/>
        </source>
    </evidence>
    <sequence length="322" mass="36122" checksum="C1BC1B74A095E7DF" modified="2020-08-12" version="1">MDCRPTGSVIGIAVFAWLWLLAAGRGQPQSLTLGGYQQYQQTATLGGYFLDNGTHYLLYERPRVTRPSFLPLSYFGNISSNPGVNALETLDYLPTRIVNGKRIKCSRAPYQCAISYQKYFICGCVILNRSWILTAHHCIVGAAGNYQVRAGTTQQRRGGQLRNVKKIVAHAGYSDYTMRNDLAMMKLSKPLKYNRCLKPVRLPSRKIRRFPRCYLVSGWGLTSANAQNVQRYLRGVRVCKVRRGKCQKKYKGTGVKIYKDMICAASPGKDSCSGDSGGPLVHGNILYGVVSFGIGCANRKYPGVYVNVKRYVPWIKKVMRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEG8</accession>
    <name>A0A6I8UEG8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Talin-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">rhea</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
            <tissue evidence="9 10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354214.4">
        <property type="nucleotide sequence ID" value="XM_001354178.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241258.1">
        <property type="nucleotide sequence ID" value="XM_033385367.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241259.1">
        <property type="nucleotide sequence ID" value="XM_033385368.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814055"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UEG8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10569">
        <property type="entry name" value="FERM_C_Talin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17089">
        <property type="entry name" value="FERM_F0_TLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17090">
        <property type="entry name" value="FERM_F1_TLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12150">
        <property type="entry name" value="talin-RS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000023">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000010">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000011">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000012">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000207">
        <property type="entry name" value="Rhea, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000005">
        <property type="entry name" value="Talin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000002">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000003">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1410.10:FF:000001">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000001">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000002">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.10:FF:000007">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000007">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000028">
        <property type="entry name" value="Talin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.10">
        <property type="entry name" value="Talin, central domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032425">
        <property type="entry name" value="FERM_f0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037438">
        <property type="entry name" value="Talin1/2-RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057346">
        <property type="entry name" value="Talin1/2_VBS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015224">
        <property type="entry name" value="Talin_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036476">
        <property type="entry name" value="Talin_cent_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054082">
        <property type="entry name" value="Talin_IBS2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049108">
        <property type="entry name" value="Talin_R4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054060">
        <property type="entry name" value="TLN1-like_RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015009">
        <property type="entry name" value="Vinculin-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981:SF1">
        <property type="entry name" value="RHEA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19981">
        <property type="entry name" value="TALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16511">
        <property type="entry name" value="FERM_f0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21896">
        <property type="entry name" value="Talin_IBS2B"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09141">
        <property type="entry name" value="Talin_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21692">
        <property type="entry name" value="Talin_R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25177">
        <property type="entry name" value="Talin_VBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21865">
        <property type="entry name" value="TLN1-like_RS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08913">
        <property type="entry name" value="VBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="88"/>
            <end position="412"/>
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            <begin position="2297"/>
            <end position="2538"/>
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            <begin position="1806"/>
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        <location>
            <begin position="2640"/>
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            <begin position="2851"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
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        <source>
            <dbReference type="PROSITE" id="PS50945"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354214.4"/>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033241258.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033241259.1"/>
        </source>
    </evidence>
    <sequence length="2865" mass="309791" checksum="4ED5C5454316668D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFV0</accession>
    <name>A0A6I8UFV0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Tyrosine aminotransferase</fullName>
            <shortName evidence="13">TAT</shortName>
            <ecNumber evidence="5 13">2.6.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Tat</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-tyrosine + 2-oxoglutarate = 3-(4-hydroxyphenyl)pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15093"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="EC" id="2.6.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001354787.4"/>
    </dbReference>
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        <property type="reaction ID" value="UER00338"/>
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        <property type="component" value="Chromosome X"/>
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    <dbReference type="GO" id="GO:0004838">
        <property type="term" value="F:L-tyrosine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:L-phenylalanine catabolic process"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
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    </dbReference>
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        <property type="entry name" value="Aminotransferase_I/II_large"/>
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        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
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    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
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    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
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        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005958">
        <property type="entry name" value="TyrNic_aminoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005957">
        <property type="entry name" value="Tyrosine_aminoTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01264">
        <property type="entry name" value="tyr_amTase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01265">
        <property type="entry name" value="tyr_nico_aTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744:SF2">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ACCSYNTHASE"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="10 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="16">
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            <begin position="106"/>
            <end position="469"/>
        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="11"/>
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    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="14">
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            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00047798"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF00155"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001354823.2"/>
        </source>
    </evidence>
    <sequence length="499" mass="55526" checksum="92EB68FAF752885B" modified="2020-08-12" version="1">MPSSVAKEGYQFQRANSRNQQSSSCPSRSASGSPAAVTPPICDDVVDEALAELQLQSQQQKQKQMIHSVKGRSGWQIKSSKLAFNTHNRIRNIVESLKIKPNPEKPMIPLSIGDPTTFGNLKAADETMKAVLHSLESGKFNGYAHTQGHEASRQAVAQYSAHQRPEGAIETSDVVLCSGCSSALEYCILALADRGQNVLIPRPGFCLYHTLAEGLDIEVRYYDLLPDQQWRADLVQLEGLIDENTAALLINNPSNPCGSVFDEKHLRQLIDICERHYLPIIADEIYEHFVFPGSKHLAVSSLTREVPVLSCGGLTKRFLVPGWRMGWIIVHDRKQRLGNAVIGLKNMCGRILGSNTIIQGALPEILAKTPQSYFDGVIQVLYSNASLAYKMLKQVRGLNPVMPNGAMYMMIGVSIERFPAFKDDTHFVQELVNEQSVFCLPGSCFEYPGYVRIVLTVPLAMIEEACVRIAEFCERHYKKEDSSRSFIEHGLLDEEELGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH71</accession>
    <name>A0A6I8UH71_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Mucin-5AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4815576</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_001355418.4"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026151417" evidence="3">
        <location>
            <begin position="28"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="417"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="632"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="853"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="914"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="985"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1069"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1187"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="197"/>
            <end position="208"/>
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    <sequence length="1332" mass="144888" checksum="141555D6C0D42B93" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHU5</accession>
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            <begin position="27"/>
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            <begin position="34"/>
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</entry>
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            <begin position="1"/>
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            <begin position="86"/>
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        <source>
            <dbReference type="RefSeq" id="XP_001357341.3"/>
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    <sequence length="356" mass="39959" checksum="4C863E87290A39F4" modified="2020-08-12" version="1">MWLTPIVVGCLVGCLATAALGYKSCAAEKQCVPSEQCNEGLLVDGHFYPDRSRTMLDEQCHYMEKCCNAHDTLPAARTLEGANSWQCGGRHDLWYYLRPWGYKQQEAKFGEFPWLVAIYGSDGYLCSGALISPRAVLTTARCVQNESAEELRLVAGEWDAAVELEPQPHQSRAVAELLLHPNYTQTPVAHNIAVLLLAKESSPFELAPNVQLICLPPPQMVYNYSQCYVSGWRRRDFGGSETLPKRWTLYALPRDQCMAKLRLSYLGRRNSRNDTLLCAGGDKGDFVCGDGGMDGVPLMCPLPGHDDRFVLAGLLARPGRCDGPQLLGIYTNLLFYRQWIDQKLRERDLDIRQYMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULP8</accession>
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        <name evidence="3" type="primary">LOC4800263</name>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">MV2-25</strain>
        </source>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357538.2">
        <property type="nucleotide sequence ID" value="XM_001357502.4"/>
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    <dbReference type="KEGG" id="dpo:4800263"/>
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    <dbReference type="OMA" id="VTIVMIP"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357538.2"/>
        </source>
    </evidence>
    <sequence length="77" mass="8617" checksum="53CEB29667B6ABE9" modified="2020-08-12" version="1">MPKQPSFVSRNLVTIVMVPSLIGIHLGWGYMQNNRKLVTESEQIDLPPVTFARFVWNKIAGGRDQTTAETQQTSSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULS6</accession>
    <name>A0A6I8ULS6_DROPS</name>
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        <submittedName>
            <fullName evidence="16">5-hydroxytryptamine receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">5-HT7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
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    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="nucleotide sequence ID" value="XM_001357545.4"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="7tmA_5-HT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000523">
        <property type="entry name" value="5-hydroxytryptamine receptor 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IP13425P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="465"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="180"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="365"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001357582.3"/>
        </source>
    </evidence>
    <sequence length="573" mass="61701" checksum="8449C0E34EC97A59" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN20</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein fit</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358161.2">
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026093201" evidence="1">
        <location>
            <begin position="19"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358161.2"/>
        </source>
    </evidence>
    <sequence length="122" mass="14314" checksum="D25F52147F4ECB13" modified="2020-08-12" version="1">MRFLIFLVLGTVLALAQARTLRGSNESRDESQELSPSWSHRHSSVWPCDVADHPQAYVLMHKVEKRLERIDGEPIKKRIVEYVTDQLRQCKSHNHMDEHCVKRSIGYAMSFINHQKEQAERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN49</accession>
    <name>A0A6I8UN49_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Fatty-acid amide hydrolase 2-B</fullName>
        </submittedName>
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        <name evidence="3" type="primary">LOC4800956</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358136.3">
        <property type="nucleotide sequence ID" value="XM_001358099.4"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF3">
        <property type="entry name" value="AT07710P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="63"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358136.3"/>
        </source>
    </evidence>
    <sequence length="533" mass="58440" checksum="8767D88D1D3ED9D4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN77</accession>
    <name>A0A6I8UN77_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4801008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358181.4">
        <property type="nucleotide sequence ID" value="XM_001358144.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN77"/>
    <dbReference type="FunCoup" id="A0A6I8UN77">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801008"/>
    <dbReference type="InParanoid" id="A0A6I8UN77"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026350489" evidence="3">
        <location>
            <begin position="26"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358181.4"/>
        </source>
    </evidence>
    <sequence length="233" mass="25398" checksum="3304C571CBADA99F" modified="2020-08-12" version="1">MVDGKSITYCSLFLLVLLLADCSHAKPSEDPSSTDSELSDLSGSEPRNKTSVRAAAAAGEESVDSDADSGSSELTANSYPHRDEDQLNSATNMVLARNYKYDVEILESGDAEESEEESRLAASEELEERFKGHGVLATDTEAFTAENIEMTPVDVEADAISESHMLLGIVVVVLALVSVCLYAGLVIWRSHLEQRYGMRERLVNRDLEEEGDGIDDVDYHVYAPTTTPTTTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPE9</accession>
    <name>A0A6I8UPE9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4801741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358784.1">
        <property type="nucleotide sequence ID" value="XM_001358747.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPE9"/>
    <dbReference type="FunCoup" id="A0A6I8UPE9">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="GeneID" id="4801741"/>
    <dbReference type="KEGG" id="dpo:4801741"/>
    <dbReference type="InParanoid" id="A0A6I8UPE9"/>
    <dbReference type="OMA" id="PQTAWLN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079178">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd08946">
        <property type="entry name" value="SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF11">
        <property type="entry name" value="LD23561P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358784.1"/>
        </source>
    </evidence>
    <sequence length="371" mass="41856" checksum="1C83C697CA2364CA" modified="2020-08-12" version="1">MSEKPTVLILGGCGFIGRNLLTYLLDNELTQEIRIADKTPPQMAWLNEEQTRAFESDKVEFCSANLINAASVRLVFAPHPTTGRAWDVVINCAAETRANQDDSVYKEGILKLSLNCANEAATQRVKRYVELSSGCVNSSDKTPLKEDCKLEPWTGVAKQKLKVEKELSGINDLSYTVVRLPIVYGIGDKRYLMPRIIIAAIYKYLNETMKLLWNDAMRLNTVHVSDVCAAIWQLAQSPKTNGQVYNIVDDSASTQGSISNLLVDIFNINVDYFGIVMSSLTKLYPTDTVGEINDKHMAPWAEICQRNGIENTPLTPYLDEEQLHHKHLYLDNTKLKDFGYVLKQPRLTRDLLMEMIDDYAKLQLFPKSLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPG2</accession>
    <name>A0A6I8UPG2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ran-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RanBP3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358631.1">
        <property type="nucleotide sequence ID" value="XM_001358594.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPG2"/>
    <dbReference type="FunCoup" id="A0A6I8UPG2">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="GeneID" id="4801559"/>
    <dbReference type="KEGG" id="dpo:4801559"/>
    <dbReference type="InParanoid" id="A0A6I8UPG2"/>
    <dbReference type="OMA" id="KTNMPHV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070229">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13180">
        <property type="entry name" value="RanBD_RanBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF142">
        <property type="entry name" value="RAN-BINDING PROTEIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="4">
        <location>
            <begin position="254"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="223"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="393"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="476"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358631.1"/>
        </source>
    </evidence>
    <sequence length="487" mass="51749" checksum="EE71890CE3A9C9E1" modified="2020-08-12" version="1">MSENNEASVESNCFRGGFTLKASRLGGAVLRPPVLGPSVLGSSAQTNSNNSSTSLAGNNEKSEDENISNNPFLREEKDEGNEDAGADATAVAAAAPTVAPESSSIEKAEQKDDDEVDERPDPLTKLRSNGIERSSMFGAAKTNMPHVQTTGYVFGQNVHERVVGVGDGHVAAAVPGSSSEKTNTDQDNVEAAAATSQSQQTQEPSSSSQLLFSSVIQKAAQSTDSSEAASSSSSSNNNKSEASEAKSLTDVAREYEESRAQKRKYEEVETFTGEEDELNIVDVSCKLFAWVNSNWEERGRGSLRLNDVKGERDCSRVVFRTSGNLRLLLNTKVWAAMVAERASPKSLRLTAIDNTGSLKIFLAMGRPADIAQLHKSLGDRIARRKLIHPEECSVENTKNGVSSEVAASLQPESTTHDDEDSSSAPAATAVAEADSMEPSPKKVLVQQPDSSDDVCVPPMEVASEDVDADAAANAEAEAKESSSTDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPH0</accession>
    <name>A0A6I8UPH0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein D3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4801664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358716.2">
        <property type="nucleotide sequence ID" value="XM_001358679.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPH0"/>
    <dbReference type="FunCoup" id="A0A6I8UPH0">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801664"/>
    <dbReference type="InParanoid" id="A0A6I8UPH0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358716.2"/>
        </source>
    </evidence>
    <sequence length="179" mass="20210" checksum="1402050E236CE5FB" modified="2020-08-12" version="1">MEDIVPDVVDSVPKGRLQVMYGGNLFVEQGNALTPTQVKDEPKVTWEGDEGQDRDTLLTLLMVDPDAPSRADPEFREILHWAMVNIPGDDPCRGYALAEYIGAGPPLNTGLHRYVFLLYRQREKIEQTATIPKTIRKGRLNFSARDFASKHRLGSPIAANYFQAQYDDYVPIRNKLFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPX7</accession>
    <name>A0A6I8UPX7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">eEF1alpha2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358993.1">
        <property type="nucleotide sequence ID" value="XM_001358956.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPX7"/>
    <dbReference type="SMR" id="A0A6I8UPX7"/>
    <dbReference type="FunCoup" id="A0A6I8UPX7">
        <property type="interactions" value="912"/>
    </dbReference>
    <dbReference type="GeneID" id="4801981"/>
    <dbReference type="KEGG" id="dpo:4801981"/>
    <dbReference type="InParanoid" id="A0A6I8UPX7"/>
    <dbReference type="OMA" id="AIRDMGM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075080">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000090">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="5"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001358993.1"/>
        </source>
    </evidence>
    <sequence length="462" mass="50631" checksum="A45D77674362A93B" modified="2020-08-12" version="1">MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEARYEEIKKEVSSYIKKIGYNPASVAFVPISGWHGDNMLEPSEKMPWFKGWTVERKEGKGEGKCLIDALDAILPPQRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILKPGMVVNFAPVNLVTEVKSVEMHHEALTEAMPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQIANGYTPVLDCHTAHIACKFAEIKEKCDRRTGKTTETEPKAIKSGDAAIIVLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKSVNFKETTSGKVTKAAEKAQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQC1</accession>
    <name>A0A6I8UQC1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359125.3">
        <property type="nucleotide sequence ID" value="XM_001359088.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQC1"/>
    <dbReference type="KEGG" id="dpo:4802148"/>
    <dbReference type="InParanoid" id="A0A6I8UQC1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006631">
        <property type="entry name" value="DM4_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398">
        <property type="entry name" value="AGAP007094-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398:SF7">
        <property type="entry name" value="LP19941P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07841">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00718">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026236468" evidence="1">
        <location>
            <begin position="30"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359125.3"/>
        </source>
    </evidence>
    <sequence length="244" mass="27629" checksum="1B97EA2F73EF544D" modified="2020-08-12" version="1">MRRLLTTYHFNLLVLYLLALGQVRVRVQAEAQTKNLDQSLALTGANATQVEAKVLRRHKRYLAFPDGSSVSAAICMTIGMIGNPDVDFLSWAVNWGVAYDLPNREWAVQHAHGLNATLAKDVIKRRSRRAFYDEVQSIFNNMGFNGHSCVARALCESARFMLPQEERGNMLQELVRTVFSLPSTPVEAQEPQAHHQYDRIYRRSKRSSKECHEIYPGCQFSLLALALGKYMAATTTKLSAFNFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQE8</accession>
    <name>A0A6I8UQE8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">F-box/LRR-repeat protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Fbxl7</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359268.3">
        <property type="nucleotide sequence ID" value="XM_001359231.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQE8"/>
    <dbReference type="FunCoup" id="A0A6I8UQE8">
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            <end position="459"/>
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            <begin position="184"/>
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            <begin position="350"/>
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            <begin position="382"/>
            <end position="394"/>
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        <source>
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    <sequence length="787" mass="84951" checksum="BF3E3F6E987EA167" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQI0</accession>
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        <submittedName>
            <fullName evidence="6">FERM and PDZ domain-containing protein 4 isoform X1</fullName>
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        <name evidence="6" type="primary">LOC4802398</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Brachycera</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="6">MV-25-SWS-2005</strain>
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            <end position="93"/>
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        <location>
            <begin position="1413"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001359323.4"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR19</accession>
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            <fullName evidence="6">Tetraspanin</fullName>
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        <name evidence="8" type="primary">Tsp96F</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
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        </subcellularLocation>
    </comment>
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        <text evidence="2 6">Belongs to the tetraspanin (TM4SF) family.</text>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079365">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CD151_like_LEL"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF551">
        <property type="entry name" value="RE08073P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359530.4"/>
        </source>
    </evidence>
    <sequence length="268" mass="29920" checksum="9DA0BFB36A69DEE2" modified="2020-08-12" version="1">MGLKGCCSCVKYLMVLINILFWLIGLSIVITSVWMLTDPTFMLSMTQSYNHYHIALYVFLGIGILITLGAFFGCWGVCRESQCLLVSFFCVILIVMVAQIAAGAWAFHNKDKLDDIVRAAVKSSVQEEYGQSTMSSRTVTFDTLQKNLKCCGADGPGDWATSRFNNVDRTNIVEIAVSSMNVFYNIPESCCKENLKDNECELSRRLKFGGPLNTAIYQQGCVDKLIEIIYENWVTIFAITAAVILLELLSLTFALSLCCAVRNQHYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URA6</accession>
    <name>A0A6I8URA6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein prd1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prd1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359671.4">
        <property type="nucleotide sequence ID" value="XM_001359634.5"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8URA6">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802835"/>
    <dbReference type="InParanoid" id="A0A6I8URA6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053015">
        <property type="entry name" value="PH_domain-containing_M2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057288">
        <property type="entry name" value="PH_PLEKHM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46556">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY M MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46556:SF1">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY M MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23142">
        <property type="entry name" value="PH_PLEKHM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="1052"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="466"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="594"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1226"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="475"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="645"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="678"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="699"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1226"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1258"/>
            <end position="1271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359671.4"/>
        </source>
    </evidence>
    <sequence length="1385" mass="152863" checksum="5B3C7D3BCF64B021" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URF3</accession>
    <name>A0A6I8URF3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 135-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4802643</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM135 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359523.2">
        <property type="nucleotide sequence ID" value="XM_001359486.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URF3"/>
    <dbReference type="FunCoup" id="A0A6I8URF3">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802643"/>
    <dbReference type="InParanoid" id="A0A6I8URF3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026749">
        <property type="entry name" value="Tmem135"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031926">
        <property type="entry name" value="TMEM135_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15982">
        <property type="entry name" value="TMEM135_C_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein 135 N-terminal" evidence="7">
        <location>
            <begin position="15"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359523.2"/>
        </source>
    </evidence>
    <sequence length="455" mass="50937" checksum="E4B8D37A2BEB3C1E" modified="2020-08-12" version="1">MAAQSKLLEAAFECSCHAYVHPWTSSCAAAAAGMMLAEVAPSFRTYSTVYLLALIMRMRVPRLQDLWRTLTGILQSTAFLSMNGGLFIFAVCLLRQLLGRFYFPTVAFLPSFLSSWLSIAVERPERRTPLAMYVANVGIETVWKMLESRGWVRSLPNGQVLIMGLSITALMFLYRLGLHKTVAKDATFKGLSVILGKEEEGPLKTPTVTTTQSSRQRPLNFQSISAYVQVYDRLRTAKHPSCPHRQGCAPYALYGGLKPFLGGLGLQVGLKLLLNISKIVNMKMQWRKQIFNKGSLQLGLALGSFSLLYKTISCGLRHTCGHDNALFAIPAGLIGSIGLLRFPNTTVAMYLMWKTLQLLYNWGIAEGKLPEVPYFNTIMYGFFTGVLFHAAILEARTLRPSYYNFLMKISGNRISRFNVLPFQAYGLKSQDQINYVIKKLKIDMTSPFPIYPLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URI1</accession>
    <name>A0A6I8URI1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">abs</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX41 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001359629.2">
        <property type="nucleotide sequence ID" value="XM_001359592.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URI1"/>
    <dbReference type="FunCoup" id="A0A6I8URI1">
        <property type="interactions" value="2465"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802769"/>
    <dbReference type="InParanoid" id="A0A6I8URI1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17951">
        <property type="entry name" value="DEADc_DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000449">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000657">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044113">
        <property type="entry name" value="DEADc_DDX41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="208"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="403"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001359629.2"/>
        </source>
    </evidence>
    <sequence length="619" mass="69164" checksum="51959F366F8C756C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URU2</accession>
    <name>A0A6I8URU2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome c oxidase assembly protein COX16 homolog, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">l(3)neo43</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COX16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359901.2">
        <property type="nucleotide sequence ID" value="XM_001359864.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URU2"/>
    <dbReference type="FunCoup" id="A0A6I8URU2">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="GeneID" id="4803103"/>
    <dbReference type="KEGG" id="dpo:4803103"/>
    <dbReference type="InParanoid" id="A0A6I8URU2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073341">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020164">
        <property type="entry name" value="Cyt_c_Oxase_assmbl_COX16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130:SF14">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX16 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14138">
        <property type="entry name" value="COX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359901.2"/>
        </source>
    </evidence>
    <sequence length="104" mass="12337" checksum="BDED49FD5CC3DF78" modified="2020-08-12" version="1">MSFSDKLKYYSKRKSFKYGVPFLIMMVAGSFGLQQFSNLRYQYSKKQPVTPDEMKKYGVNMKKRQEVTLETEYDKVRAVNIDEWENKRGPRPWEEEGLSSPAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USC0</accession>
    <name>A0A6I8USC0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein Xport-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Xport-A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359883.3">
        <property type="nucleotide sequence ID" value="XM_001359846.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USC0"/>
    <dbReference type="FunCoup" id="A0A6I8USC0">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803084"/>
    <dbReference type="InParanoid" id="A0A6I8USC0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359883.3"/>
        </source>
    </evidence>
    <sequence length="121" mass="13825" checksum="78D1385E48F3688E" modified="2020-08-12" version="1">MKSKKSAFANTSTGYGRHKHSGHGKGHGHGQGQSGKDKAEGGGKGKSREVKDQHEVKEEQDKDLNSGFGDYLRTPEAFEMMKLFVFANTIMLIVTMAWPHIKEQFYMINHWLETFREDHRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUZ8</accession>
    <name>A0A6I8UUZ8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">mle</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001361331.3">
        <property type="nucleotide sequence ID" value="XM_001361294.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UUZ8">
        <property type="interactions" value="1577"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804832"/>
    <dbReference type="InParanoid" id="A0A6I8UUZ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UUZ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033679">
        <property type="term" value="F:3'-5' DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17972">
        <property type="entry name" value="DEXHc_DHX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19854">
        <property type="entry name" value="DSRM_DHX9_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19855">
        <property type="entry name" value="DSRM_DHX9_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000026">
        <property type="entry name" value="ATP-dependent RNA helicase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000028">
        <property type="entry name" value="ATP-dependent RNA helicase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000677">
        <property type="entry name" value="ATP-dependent RNA helicase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000025">
        <property type="entry name" value="Dosage compensation regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000284">
        <property type="entry name" value="probable ATP-dependent RNA helicase YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044447">
        <property type="entry name" value="DHX9_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044445">
        <property type="entry name" value="DHX9_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044446">
        <property type="entry name" value="DHX9_DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF119">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="12">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="12">
        <location>
            <begin position="168"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="392"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="638"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1296"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1307"/>
            <end position="1318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001361331.3"/>
        </source>
    </evidence>
    <sequence length="1318" mass="144714" checksum="63EE6EE377212E98" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVL4</accession>
    <name>A0A6I8UVL4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytosol aminopeptidase</fullName>
            <ecNumber evidence="7">3.4.13.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinylglycine-S-conjugate dipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Leucine aminopeptidase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proline aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Prolyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">S-Lap7</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides. The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme. For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates. Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-cysteinylglycine + H2O = L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:28783"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:28784"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-benzyl-L-cysteinylglycine + H2O = S-benzyl-L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:62568"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:145802"/>
            <dbReference type="ChEBI" id="CHEBI:145803"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:62569"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an S-substituted L-cysteinylglycine + H2O = an S-substituted L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:60444"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.13.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:60445"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.23" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001361611.2">
        <property type="nucleotide sequence ID" value="XM_001361574.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVL4"/>
    <dbReference type="FunCoup" id="A0A6I8UVL4">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="GeneID" id="4805161"/>
    <dbReference type="KEGG" id="dpo:4805161"/>
    <dbReference type="InParanoid" id="A0A6I8UVL4"/>
    <dbReference type="OMA" id="MFAQATV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072265">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF16">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 18" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M17 leucyl aminopeptidase N-terminal" evidence="16">
        <location>
            <begin position="39"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="15">
        <location>
            <begin position="201"/>
            <end position="512"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014190"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030997"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001361611.2"/>
        </source>
    </evidence>
    <sequence length="524" mass="56879" checksum="E0B1A6406286098B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWH9</accession>
    <name>A0A6I8UWH9_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4805538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361925.2">
        <property type="nucleotide sequence ID" value="XM_001361888.4"/>
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    <dbReference type="GeneID" id="4805538"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361925.2"/>
        </source>
    </evidence>
    <sequence length="111" mass="12450" checksum="77F465572E6132D1" modified="2020-08-12" version="1">MIYGMLVTLSTAIYTTVATLLQLQLTLINWMMSFSLFILGLFCKPYLIVPLLAAGFFMVRRILLRRAKRTQAWKSPGNKESGSSMHYSKKTPPSRDTESSLPNSVGDTSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY10</accession>
    <name>A0A6I8UY10_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Adult cuticle protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132565.2">
        <property type="nucleotide sequence ID" value="XM_002132529.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8UY10"/>
    <dbReference type="KEGG" id="dpo:6902829"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031874">
        <property type="entry name" value="Cuticle_Acp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12336">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12336:SF0">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15955">
        <property type="entry name" value="Cuticle_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026327118" evidence="1">
        <location>
            <begin position="20"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132565.2"/>
        </source>
    </evidence>
    <sequence length="136" mass="13317" checksum="8DAF6E6C64E1C4B9" modified="2020-08-12" version="1">MKFAVAVVFTLALAMGVQSSVIPLLSQVAGHGLSYTAVSGPAVIASPWAHAAAPWAHAAAPWGHPAAAWPAAVTVGAWPPAAPAVVAAHSPAVVVGPVAHEGVYTAQTRGAIHTAPLAGHIQSVASINAAPAPGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ77</accession>
    <name>A0A6I8UZ77_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kinesin-associated protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Kap3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133912.2">
        <property type="nucleotide sequence ID" value="XM_002133876.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ77"/>
    <dbReference type="FunCoup" id="A0A6I8UZ77">
        <property type="interactions" value="626"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901211"/>
    <dbReference type="InParanoid" id="A0A6I8UZ77"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008658">
        <property type="entry name" value="KAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605:SF2">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05804">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01297">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="972"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="972"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133912.2"/>
        </source>
    </evidence>
    <sequence length="1055" mass="119779" checksum="175A6AD5728AC92D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZQ2</accession>
    <name>A0A6I8UZQ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein TwdlZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TwdlZ</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133723.2">
        <property type="nucleotide sequence ID" value="XM_002133687.3"/>
    </dbReference>
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        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902196"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DUF243"/>
    </dbReference>
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        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
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    </dbReference>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026052779" evidence="2">
        <location>
            <begin position="21"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="3">
        <location>
            <begin position="34"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133723.2"/>
        </source>
    </evidence>
    <sequence length="213" mass="23597" checksum="96B78348C429675B" modified="2020-08-12" version="1">MLNLWCLLLVSGLGLNAAQGSRSYLPPPATAVEPIITKQFYSISPAEDPEELQPRTKHLVIGQPRRNYRVIFIRAPTPSSDQVKYTAELAPQEERTVIYVLARKQQELRAQDIVAPQQQAAEQKPDIFFIKYKTNEEAAAAQHEIQTQYDQLGGNTEIAAPYVAPVQSVIGALSPATQQTPPGPDGDGGYHYQRPLPMPLPMPLSMPLGSWRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V089</accession>
    <name>A0A6I8V089_DROPS</name>
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        <submittedName>
            <fullName evidence="4">DnaJ protein ERDJ2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6900445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134838.3">
        <property type="nucleotide sequence ID" value="XM_002134802.3"/>
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    <dbReference type="KEGG" id="dpo:6900445"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="92"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002134838.3"/>
        </source>
    </evidence>
    <sequence length="196" mass="21456" checksum="E06C43EFC1470EC9" modified="2020-08-12" version="1">MSGKEFASEENFGSSLLLVLLAMVFVLTFTTLGCWSRNASGRPARKALAARSAAWNVLGSCALVLFVVVAWTMVTIVGVGLIGRTRSGNAFNPHKILQVPSSCSQAEIRKAYRELSKVLHPDVRGTGDEAQFILLSTAYRALIKSPAKENYRLFGHPDGSDALGADRPEALGRFYNWVTQSVQSFGQWLRTKFGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2J2</accession>
    <name>A0A6I8V2J2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tenascin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6897056</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137240.3">
        <property type="nucleotide sequence ID" value="XM_002137204.4"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:6897056"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF106">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026095382" evidence="6">
        <location>
            <begin position="29"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="34"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="90"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="146"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="201"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="257"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="313"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="369"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="432"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002137240.3"/>
        </source>
    </evidence>
    <sequence length="492" mass="53914" checksum="54010C7475133C12" modified="2020-08-12" version="1">MKSHQVRMFYHVCSLWTLLCLMPWPSLAAMSRDSSNCIEGSVEADPQDCASYFQCLDTVIVHQQCPAGTYYEASYQVCVIDDKGICTPASNKCIEGELAEDPADCAGYLQCIDGDFVKQKCALGSYFNLISKICLVDGNVSCSPQQERCNDGELSADPEDCARYLQCVNGVLVKERCNKGSYFDVNFNMCVVDVDGVCVSSENCTEGELESDPNDCAGYLKCIDGYLEEQQCSSGSYFNSSLKICLVDVNGICVAPPANCTEGELDVDPNDCAGYLKCINGQFVEEQCPSGSYFDAKMELCLIDVDGICLPTVRKCTEGYMEEDPEDCAGYRHCINQELVQLKCDPGSYFNATESGCLIDEDGVCVSLVERCSEGELMQDAANCGGFLICVDGLFVAKNCGSGEYFHPGVQECLEDDRNICHSKCRCVSSVKDRCVEGKRVADPQDCGAYLECIDGQLVEQICVRGTYFETTMGGCVIDEKHFCVDGERKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2W8</accession>
    <name>A0A6I8V2W8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">trypsin</fullName>
            <ecNumber evidence="8">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6897502</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="8"/>
    <dbReference type="RefSeq" id="XP_002137651.2">
        <property type="nucleotide sequence ID" value="XM_002137615.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2W8"/>
    <dbReference type="FunCoup" id="A0A6I8V2W8">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897502"/>
    <dbReference type="InParanoid" id="A0A6I8V2W8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026290123" description="trypsin" evidence="9">
        <location>
            <begin position="23"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="60"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002137651.2"/>
        </source>
    </evidence>
    <sequence length="295" mass="33783" checksum="7A548FB7DF6B7096" modified="2020-08-12" version="1">MNPIYSRFLCLQLLLLLARGLAVKPKAVAVSDSNLHVRPAVRTVNTNGFQLTEGGDVGYWLVHIMNGQRFICGASYYSALYVLTSANCMYRYRKSLNTMSVEYVNPRMDQENKIAYIESVSVPKNYQYPDNYMDIAVVKLRRNLTGSPDTVRLCSQPLRRYPKFTAVACGVDPGGSVDRTATDDVAIMPKRFCQPLYNKDKLRISETIACVREWNNYRYRMYEFGCPVTAGNNELCGIVAWGPQFESEHPGLFTDIYQVKGFILKVVRGVYGDSRQLSKRTRRIRPKKMHRIWAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3B8</accession>
    <name>A0A6I8V3B8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein C15orf61 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6896977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137163.1">
        <property type="nucleotide sequence ID" value="XM_002137127.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3B8"/>
    <dbReference type="FunCoup" id="A0A6I8V3B8">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="GeneID" id="6896977"/>
    <dbReference type="KEGG" id="dpo:6896977"/>
    <dbReference type="InParanoid" id="A0A6I8V3B8"/>
    <dbReference type="OMA" id="SHFRIGC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248094">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029245">
        <property type="entry name" value="DUF4528"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34651">
        <property type="entry name" value="SIMILAR TO ENSANGP00000021391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34651:SF1">
        <property type="entry name" value="SIMILAR TO ENSANGP00000021391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15031">
        <property type="entry name" value="DUF4528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002137163.1"/>
        </source>
    </evidence>
    <sequence length="95" mass="11190" checksum="B8054A240ED9BEF8" modified="2020-08-12" version="1">MSHFNWTLDTGTNYHILRTGCYPYMKYHCSRREVQDLSLEDKFFRVLKVINLGLPMLFYGLAAIRLISHTEIVHVSETVKVPIYFLYAEDKGARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4Z1</accession>
    <name>A0A6I8V4Z1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neuropeptide SIFamide</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SIFa</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138929.2">
        <property type="nucleotide sequence ID" value="XM_002138893.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4Z1"/>
    <dbReference type="FunCoup" id="A0A6I8V4Z1">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="GeneID" id="6898969"/>
    <dbReference type="KEGG" id="dpo:6898969"/>
    <dbReference type="InParanoid" id="A0A6I8V4Z1"/>
    <dbReference type="OMA" id="SWFTQEQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246462">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026175456" evidence="1">
        <location>
            <begin position="27"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138929.2"/>
        </source>
    </evidence>
    <sequence length="72" mass="7930" checksum="46F462A3F78AB839" modified="2020-08-12" version="1">MALRFTLTLLLVTILATAILLSSSEAAYRKPPFNGSIFGKRNGLDYDNAKMSAVCEVAMEACPMWFPQNDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V751</accession>
    <name>A0A6I8V751_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparaginase</fullName>
            <ecNumber evidence="2">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC26533708</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_015035210.2">
        <property type="nucleotide sequence ID" value="XM_015179724.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V751"/>
    <dbReference type="KEGG" id="dpo:26533708"/>
    <dbReference type="InParanoid" id="A0A6I8V751"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V751">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08963">
        <property type="entry name" value="L-asparaginase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.40:FF:000001">
        <property type="entry name" value="L-asparaginase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041725">
        <property type="entry name" value="L-asparaginase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="9"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="235"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="5">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015035210.2"/>
        </source>
    </evidence>
    <sequence length="360" mass="39138" checksum="44648426C561AF77" modified="2020-08-12" version="1">MSKCLNFKRVLVIYTGGTIGMTPNDNGVLVPEPKAFERFIRKDVSLHDPSLQVPDGFLALPLVKDQSVRVIYQFIEYDPLLDSSNMAGKDWQRIAQDIGRTYDLFDGFVVLHGTDTLAYTSSALSFFLENLDKPVVVTGSMIPIYETRTDGRNNFVSSLIISGCYKIPEVCVVFANKLLRGNRTVKVNCNSLDAFDSPNAPLLGTFEGDIIINNFLVRPGPGARPLAVTGEFQMNVASLRITPDMSLDLIRSALAEPIMGVVLESYGSGNIPSNWSEITSLLRSAVSRGVIIVNCTQCLAGSVAAIYDAGAVLSDLGVLSGSDMTSEAAYTKLAYVLGTDLPHHNKIELMKMNLRGEITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7L5</accession>
    <name>A0A6I8V7L5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4817871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035362.2">
        <property type="nucleotide sequence ID" value="XM_015179876.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V7L5">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817871"/>
    <dbReference type="InParanoid" id="A0A6I8V7L5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V7L5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052752">
        <property type="entry name" value="NACHT-WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871">
        <property type="entry name" value="BETA TRANSDUCIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871:SF37">
        <property type="entry name" value="GH25853P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="474"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015035362.2"/>
        </source>
    </evidence>
    <sequence length="1358" mass="152369" checksum="1DAC1032632F032C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8C3</accession>
    <name>A0A6I8V8C3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein rau</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rau</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035273.2">
        <property type="nucleotide sequence ID" value="XM_015179787.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V8C3"/>
    <dbReference type="FunCoup" id="A0A6I8V8C3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817863"/>
    <dbReference type="InParanoid" id="A0A6I8V8C3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V8C3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045742">
        <property type="term" value="P:positive regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045743">
        <property type="term" value="P:positive regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17043">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000295">
        <property type="entry name" value="AGAP008705-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000417">
        <property type="entry name" value="GD22628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21298">
        <property type="entry name" value="GH01721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21298:SF2">
        <property type="entry name" value="GH01721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="61"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="155"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015035273.2"/>
        </source>
    </evidence>
    <sequence length="450" mass="49575" checksum="3D08F47ACF92FA4A" modified="2020-08-12" version="1">MLKHVQISPLRNRSDSVSLRSSSIASSCASSMCGSPEPPTDHLQRTPSRASSYSSLNEQPPQTTIKVYTNCLKIDIEYKTLGIQWDTTSKEVIAMLLRRLKMRHRDPRLFYLSMEVAVRRAGVKTILVLDDDTRPAIMQACHPKGESRFCLQLKAGGLIRVHTSALQPSSQYKSLVISEETNCDELLQLLLGCYSSLEPVENFSLYEVCPGQECQRKLHPDDLPLRVQAERMKRGENCHFLVRRTPNYARRRQLLANLNEAINQSFENEAALATLATGDAVEDATSLLELSLESELSLSEDLRSCSSSSTEDAEDEDECASSSGSSDNSTECALRRDFYPRPTSMVSASASASAPPAPVVPSVSPARAYSPVYNIREIRTASHSFSSLGKDKKLLDIHMNARYGPAGIYSRLLPVAETEVLDLNGNATSKLAAEAVNNNPSQGLGHFVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBG6</accession>
    <name>A0A6I8VBG6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Titin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037697.2">
        <property type="nucleotide sequence ID" value="XM_015182211.2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897522"/>
    <dbReference type="InParanoid" id="A0A6I8VBG6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1000">
        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="753"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="919"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1046"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015039839.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015039841.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033234426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033234427.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="51791" checksum="F484D7ACAF42511C" modified="2020-08-12" version="1">MAETINNKPSIQIRSGLPSPLPDKSPSPATKLLISHGKPNFTIGNKSPKAEENGTSPLSPQQTASNNYNPYKSASNNGSNSNSNGTGNGTGSEINKFIVGTKFNGVFKPSTAATSWATATTNGNGSLNGNGAAAGGENEASKVVLRRPKAATSPPPAEAEDDNVPEFIRRQRRIQERLAKENVLDFENRRSGYFTHVVISPDSPNRTSFVETMASPAIVPSLEMPAPVHEKVGEEEEEEQKQHQEQEQRERATLVTTSALISEPETVAAVTNGHSEEPETTTNGHTDQEYVAPEEVAKAIEEVNKAVAGEDDDKPEAETPEPEAESVSAPAPVPVPVERKIESEDESKPKTEEIIPATAARTPSPVEAQVEVVQHVQNGETAAATAAPATNGQDTDGITISHTNGQSSDGPSIPSPDPSGALGVNYEPKTVVSFSQELGGGENNYPDTVKVVTSESSDGELKDLAKLKFDIKNDGQADDVQVTPVLRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFL3</accession>
    <name>A0A6I8VFL3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26534313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040258.2">
        <property type="nucleotide sequence ID" value="XM_015184772.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFL3"/>
    <dbReference type="KEGG" id="dpo:26534313"/>
    <dbReference type="InParanoid" id="A0A6I8VFL3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VFL3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015040258.2"/>
        </source>
    </evidence>
    <sequence length="146" mass="16576" checksum="B2BFC517BBE77869" modified="2020-08-12" version="1">MALNPKCLRVSCVIIAIGWIIGTIFTASYLVQADTEYPIVTLIMAISDFVTTAGSIMGLIGAIKNIPLALLLWLLTILISRCLELIFFMIFHLERKWLSASFYMATDRALSLILFISAMLCVTVISIFVIYEYYGYLRNKIRYNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGL1</accession>
    <name>A0A6I8VGL1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein tyn</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">tyn</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040905.2">
        <property type="nucleotide sequence ID" value="XM_015185419.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040906.2">
        <property type="nucleotide sequence ID" value="XM_015185420.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGL1"/>
    <dbReference type="KEGG" id="dpo:6901851"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01099">
        <property type="entry name" value="PAN_AP_HGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000017">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052774">
        <property type="entry name" value="Celegans_DevNeuronal_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056953">
        <property type="entry name" value="CUT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327">
        <property type="entry name" value="FI18240P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327:SF1">
        <property type="entry name" value="RE15579P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25057">
        <property type="entry name" value="CUT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="689"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="44"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="139"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="229"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="3">
        <location>
            <begin position="391"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015040905.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015040906.2"/>
        </source>
    </evidence>
    <sequence length="723" mass="81268" checksum="B6B25C158D56CFFA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGT2</accession>
    <name>A0A6I8VGT2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">T-complex protein 11-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC4815922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041011.2">
        <property type="nucleotide sequence ID" value="XM_015185525.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240140.1">
        <property type="nucleotide sequence ID" value="XM_033384249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGT2"/>
    <dbReference type="KEGG" id="dpo:4815922"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VGT2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="465"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015041011.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033240140.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="55628" checksum="97C8BDEABD2662FB" modified="2020-08-12" version="1">MENRQQQEHEQEQNQSQKLGNRTESESSVNSMTRFVVPSLDGSPPKIVTIDEVSGIFKDLRNMELAHEIALNPEFKLKQYEPPTHSFEGRVKAMVHKAYWDVLRTQLERQPPQYDQAIRLLAEIKECIPQLVTPNERTLVRINEVLDTDVIRQQAEQGVLDFKSYADFVILILSQSCSAARDEAVAELSMMDDVVDTFRSILELMAMMKLDMANFMLELARKEISANSVQYEKEKLSKYLHEFHGNVGFPATEDWLQRNRSDVSSIQTIYNGYMELIDYPEDKDFPELLRIDKERFAGISWRAQRLILCASLISIAQSPPIISQRPNNRAKLAKQLEIIMQDVKNETQVAAALESCYEQIRCLVNSALSQEQQPDMDEATLASLKSQLMQLSNKTSPVRSLMAKRFYLFVRVSLRADNHIPPPPAGFKEFEEELTALISSILRLISYNHAVFGDQFFKILEKTTPSAPESTSEPAAATPINGIAQVANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIA6</accession>
    <name>A0A6I8VIA6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylinositol-3,4-bisphosphate 4-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC6901629</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Signal transduction; phosphatidylinositol signaling pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol 3,4-bisphosphate 4-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.66" evidence="3"/>
    <dbReference type="RefSeq" id="XP_015041906.2">
        <property type="nucleotide sequence ID" value="XM_015186420.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041907.2">
        <property type="nucleotide sequence ID" value="XM_015186421.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VIA6"/>
    <dbReference type="KEGG" id="dpo:6901629"/>
    <dbReference type="UniPathway" id="UPA00944"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VIA6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016316">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000563">
        <property type="entry name" value="Phosphatidylinositol3,4-bisphosphate 4-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039034">
        <property type="entry name" value="INPP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187">
        <property type="entry name" value="AGAP000124-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,4-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="28"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="173"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="563"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015041906.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015041907.2"/>
        </source>
    </evidence>
    <sequence length="1269" mass="142268" checksum="FCFC1A11B5FBC53F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLC4</accession>
    <name>A0A6I8VLC4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26534435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015043397.2">
        <property type="nucleotide sequence ID" value="XM_015187911.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLC4"/>
    <dbReference type="KEGG" id="dpo:26534435"/>
    <dbReference type="InParanoid" id="A0A6I8VLC4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0273422">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015043397.2"/>
        </source>
    </evidence>
    <sequence length="162" mass="18645" checksum="8CF4FB0690A9CE25" modified="2020-08-12" version="1">MILANRFCYCMSLRAGCFILSIISVFVCSMHIYNFNVKMGDNHTNIDSVVFPEGVTQILFRLMPEFLTIIAVFFLLTALTTNLIHLILITLTFEVTQLLYQFLYSIVATALNINVTVRMGVYSSAVYWGFISGWIVFSAYFVYIILSYYQKTLAMMQHEVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VN68</accession>
    <name>A0A6I8VN68_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein argonaute-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6899487</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015044354.2">
        <property type="nucleotide sequence ID" value="XM_015188868.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VN68"/>
    <dbReference type="FunCoup" id="A0A6I8VN68">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899487"/>
    <dbReference type="InParanoid" id="A0A6I8VN68"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="312"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="585"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015044354.2"/>
        </source>
    </evidence>
    <sequence length="913" mass="104170" checksum="E12CC982BA5C4FD8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNY0</accession>
    <name>A0A6I8VNY0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sentrin-specific protease 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6898007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232573.1">
        <property type="nucleotide sequence ID" value="XM_033376682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNY0"/>
    <dbReference type="KEGG" id="dpo:6898007"/>
    <dbReference type="InParanoid" id="A0A6I8VNY0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.395.10:FF:000001">
        <property type="entry name" value="Sentrin-specific protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="132"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033232573.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="38067" checksum="4D8CCD22BB2D72B3" modified="2020-08-12" version="1">MDREEDIEEQDRSPPKISRYTEAQPEEISVPINHSSLSLRVESTEQCSLSFLPFYSSELGTKNGNIQKAEKCKKRILAIKKRIAVLEKQIKLNKNDAEKSMLFPLTEAQQIQIDSICSHTDEESIVIDKFKNPIQVKDLKILKSQNDWLNDKIINFYVDLISERSTRTSNPLPTVYTFSTFFVERFLTDGYKGVRRYTKKIDIFSYDMILIPENIKNIHWCMTIINLKEKTIRYYDSLGGGHDLLLHALTTYLAEESMDKRHVAYDIKEFSLETVKDMPRQENTHDCGVFACMAAEYVTRCQPLNFTQKDIPNLRSKMILEISSGRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPV3</accession>
    <name>A0A6I8VPV3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Cadherin-23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Cad88C</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033232779.1">
        <property type="nucleotide sequence ID" value="XM_033376888.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VPV3">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801364"/>
    <dbReference type="InParanoid" id="A0A6I8VPV3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001736">
        <property type="term" value="P:establishment of planar polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000344">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000345">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000347">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000306">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000382">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000098">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000124">
        <property type="entry name" value="Cadherin-related family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000168">
        <property type="entry name" value="Cadherin-related family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000039">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000118">
        <property type="entry name" value="protocadherin Fat 4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF137">
        <property type="entry name" value="CADHERIN-RELATED TUMOR SUPPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1822"/>
            <end position="1846"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="62"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="168"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="277"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="389"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="506"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="617"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="739"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="851"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="973"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="1077"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="1196"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="1299"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="1407"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="1602"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1538"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1545"/>
            <end position="1555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033232779.1"/>
        </source>
    </evidence>
    <sequence length="1978" mass="221869" checksum="ACA3FF99C67D8F96" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSX8</accession>
    <name>A0A6I8VSX8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234018.1">
        <property type="nucleotide sequence ID" value="XM_033378127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VSX8"/>
    <dbReference type="InParanoid" id="A0A6I8VSX8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VSX8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21112">
        <property type="entry name" value="CHEMOSENSORY PROTEIN A 29A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21112:SF0">
        <property type="entry name" value="CHEMOSENSORY PROTEIN A 29A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026119858" evidence="1">
        <location>
            <begin position="21"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234018.1"/>
        </source>
    </evidence>
    <sequence length="202" mass="23617" checksum="76216ED108238F32" modified="2020-08-12" version="1">MLCTTIKIWLLLGLLHGTVGERKWDYEPVSIDTYSTDESKLKVESKIDRLKRGEFGISCTVEWKYDVDETTMVEASAYRSNSGAENDYKVLPWSIPKQSYYDYLNTYYKDVLIPNLGHCSNLPQFEGKFQPPWPKDTYLVDKCVFEGQGLPEVVPPGFYKIIFITTVFRIRNEYRNFHTFGIPTFEDSIHLPTDPTRMWKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VUG6</accession>
    <name>A0A6I8VUG6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lysozyme</fullName>
            <ecNumber evidence="2">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4804422</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 22 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033234710.1">
        <property type="nucleotide sequence ID" value="XM_033378819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VUG6"/>
    <dbReference type="SMR" id="A0A6I8VUG6"/>
    <dbReference type="FunCoup" id="A0A6I8VUG6">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804422"/>
    <dbReference type="InParanoid" id="A0A6I8VUG6"/>
    <dbReference type="OMA" id="TWCRRAK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070860">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16899">
        <property type="entry name" value="LYZ_C_invert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000024">
        <property type="entry name" value="C-type lysozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001916">
        <property type="entry name" value="Glyco_hydro_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019799">
        <property type="entry name" value="Glyco_hydro_22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11407:SF63">
        <property type="entry name" value="LYSOZYME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00062">
        <property type="entry name" value="Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00135">
        <property type="entry name" value="LYZLACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00263">
        <property type="entry name" value="LYZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00128">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51348">
        <property type="entry name" value="GLYCOSYL_HYDROL_F22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="4" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026033418" description="lysozyme" evidence="10">
        <location>
            <begin position="28"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 22 (GH22)" evidence="11">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033234710.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="16702" checksum="630B880A8EE66324" modified="2020-08-12" version="1">MANQMHKSGALVLVLNLLLLTQSETEAKLLTRCQLAKELLRHDFPRSYLSNWVCLVESESGRSTSKSMQLPNQSVSYGLFQINSKNWCRKGRRGGICNIKCEEFLNDEISDDSRCAMQIFNRHGFQAWPGWMSKCRGRTLPDVSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWH2</accession>
    <name>A0A6I8VWH2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine-rich cell wall structural protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902576</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235430.1">
        <property type="nucleotide sequence ID" value="XM_033379539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWH2"/>
    <dbReference type="KEGG" id="dpo:6902576"/>
    <dbReference type="InParanoid" id="A0A6I8VWH2"/>
    <dbReference type="OMA" id="NAGCINT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026066645" evidence="2">
        <location>
            <begin position="17"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235430.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="11797" checksum="28231F124F2F5FEE" modified="2020-08-12" version="1">MKILIVLAVLVTVASALPQFGFGGPGFGGPGFGGPGFGGGGYGRPGFGGGPGFGGGFGGGPGYGGFGGGPGYGGFGGSPYGGFGGGGGRRRHGGGGGGGGGGAASASASSSASAAGGGAASSSASSSASASGGGGFYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWT2</accession>
    <name>A0A6I8VWT2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">LOC6902738</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3">MV-25-SWS-2005</strain>
            <tissue evidence="2 3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235539.1">
        <property type="nucleotide sequence ID" value="XM_033379648.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235540.1">
        <property type="nucleotide sequence ID" value="XM_033379649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWT2"/>
    <dbReference type="KEGG" id="dpo:6902738"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250436">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VWT2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033235539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235540.1"/>
        </source>
    </evidence>
    <sequence length="56" mass="5747" checksum="6C716AB41608C071" modified="2020-08-12" version="1">MCCNPGGSCGLPACIQCTNFCFNCWTGTASVPSCRPGYTPACPLYRGCCGGPRGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W067</accession>
    <name>A0A6I8W067_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Acylglycerol kinase, mitochondrial</fullName>
            <ecNumber evidence="5">2.7.1.107</ecNumber>
            <ecNumber evidence="22">2.7.1.138</ecNumber>
            <ecNumber evidence="23">2.7.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Multiple substrate lipid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">Mulk</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="32">MV-25-SWS-2005</strain>
            <tissue evidence="32">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:34071"/>
            <dbReference type="ChEBI" id="CHEBI:74938"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:43329"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41079"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:75757"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41080"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1-hexadecanoyl-sn-glycerol + ATP = 1-hexadecanoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:75542"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:43309"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + ATP = 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43316"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
            <dbReference type="ChEBI" id="CHEBI:78209"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:43317"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>N-(hexanoyl)sphing-4-enine + ATP = N-hexanoylsphing-4-enine 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43312"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63867"/>
            <dbReference type="ChEBI" id="CHEBI:82959"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:43313"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1-acyl-sn-glycerol + ATP = a 1-acyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33747"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:64683"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:33748"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 2-acylglycerol + ATP = a 2-acyl-sn-glycerol 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39847"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:64982"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:39848"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>a monoacylglycerol + ATP = a monoacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19293"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17408"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77589"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.94"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:19294"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>an N-acylsphing-4-enine + ATP = an N-acylsphing-4-enine 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17929"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57674"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.138"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:17930"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the AGK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="5"/>
    <dbReference type="EC" id="2.7.1.138" evidence="22"/>
    <dbReference type="EC" id="2.7.1.94" evidence="23"/>
    <dbReference type="RefSeq" id="XP_033236159.1">
        <property type="nucleotide sequence ID" value="XM_033380268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W067"/>
    <dbReference type="FunCoup" id="A0A6I8W067">
        <property type="interactions" value="1076"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811834"/>
    <dbReference type="InParanoid" id="A0A6I8W067"/>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047620">
        <property type="term" value="F:acylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001729">
        <property type="term" value="F:ceramide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045579">
        <property type="entry name" value="AGK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF31">
        <property type="entry name" value="ACYLGLYCEROL KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19712">
        <property type="entry name" value="AGK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 32" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="30">
        <location>
            <begin position="53"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00024636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00025749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00026096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00026098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00026142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00030553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00044480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_033236159.1"/>
        </source>
    </evidence>
    <sequence length="406" mass="45705" checksum="A05904A8B44EABEF" modified="2020-08-12" version="1">MNILRVVRNNWKKSAFAAAVSGYALSSLKTHIEITQYMSEFSTNLSSTSENVGAPKNVLVVMNPIANKKKSEKTFKKYCEPILHLAGYSVEILRTNHIGHAKAYIEEMANLPDAIVIAGGDGTSSEVVTGLMRRNENVCPITILPLGRTNQATHKYFQIGVQSELDYIKSLCSALLPMLKDDFMYQSVIRYDVINNDDDSEKHLKPIFGLNGFSWGLLKDIDSTKEKYWYFGPIRHYVAAVSKLFSKIWSLETDYVYTPPCAGCLDCVEVRNEESRFLNNFLGKLVNPNRSNMKPQRQLVKNSECSSKQQGKMEANQIDINCIQNSENFSELETQFISSLQPGWDFIKTIPSITSSTIVPQLVLRSRTIQLHPTGETTNVYSIDGEEYDARPIKLSVVPNAIKVFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0R3</accession>
    <name>A0A6I8W0R3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tubulin polyglutamylase TTLL4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TTLL4A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236339.1">
        <property type="nucleotide sequence ID" value="XM_033380448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0R3"/>
    <dbReference type="FunCoup" id="A0A6I8W0R3">
        <property type="interactions" value="579"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26533298"/>
    <dbReference type="InParanoid" id="A0A6I8W0R3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000009">
        <property type="entry name" value="tubulin polyglutamylase TTLL5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF162">
        <property type="entry name" value="TUBULIN MONOGLUTAMYLASE TTLL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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        <property type="component" value="Chromosome X"/>
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            <begin position="7"/>
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            <begin position="254"/>
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            <begin position="435"/>
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        <location>
            <begin position="37"/>
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        <location>
            <begin position="76"/>
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        <location>
            <begin position="211"/>
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    </feature>
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        <location>
            <begin position="264"/>
            <end position="281"/>
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    </feature>
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            <begin position="324"/>
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            <begin position="435"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033237475.1"/>
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</entry>
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    <accession>A0A6I8W2L7</accession>
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        <submittedName>
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                <consortium name="RefSeq"/>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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    </reference>
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        <property type="nucleotide sequence ID" value="XM_033381048.1"/>
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        <property type="nucleotide sequence ID" value="XM_033381050.1"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN TINCAR"/>
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            <begin position="91"/>
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            <begin position="725"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4F6</accession>
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        <submittedName>
            <fullName evidence="3">Protein roadkill</fullName>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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    </reference>
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    </dbReference>
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            <begin position="1"/>
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        <location>
            <begin position="82"/>
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            <begin position="1"/>
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        <location>
            <begin position="17"/>
            <end position="29"/>
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            <begin position="37"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238235.1"/>
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    </evidence>
    <sequence length="299" mass="30922" checksum="644739DD51AE4F44" modified="2020-08-12" version="1">MTSGPGGGGGSGQPAHSSATAATATTVSSNSRCNTPSTAAATSTAATASSGAGTLQRRILRGHAAQTTSGERVLLINYVPQSGSQTGSGAASTSAGSNHSTSQLPTRKILQARATAGAAAAAAAGGGATASSSTASGTSTSTPAVSFAGLGSEVTVTLTRTNQRASVTSVTAAGHIVRNQQSAYHQTTTTATASTSTSTTAMHEPIQHIATPTTPPPLVFTHHPHHIHYHQEDQLVDHHQLEQEQMNIDHNQQTEADQLIEYDGGVIEEQHHHQMQLQEDLHDPQQQQQHEVVQEVYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5I4</accession>
    <name>A0A6I8W5I4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein argonaute-2-like isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="5">Protein argonaute-2-like isoform X3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC6902030</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237942.1">
        <property type="nucleotide sequence ID" value="XM_033382051.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237944.1">
        <property type="nucleotide sequence ID" value="XM_033382053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5I4"/>
    <dbReference type="KEGG" id="dpo:6902030"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244367">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Protein argonaute N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033237942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033237944.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="15483" checksum="0885B8D964345E64" modified="2020-08-12" version="1">MPAVAYHYDVKITPERPKKFYRQAFEQYRVEHLGGAIAAFDGRASCYSVVKLKCSSQGQEVKVTDRHGRTLNYTLELKETEDLEVDLNSLRSFGFLFVSCLLASSFYSRMFCSFLFGRVIMNMNVECPYLCRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5W6</accession>
    <name>A0A6I8W5W6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117184463</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238069.1">
        <property type="nucleotide sequence ID" value="XM_033382178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5W6"/>
    <dbReference type="KEGG" id="dpo:117184463"/>
    <dbReference type="InParanoid" id="A0A6I8W5W6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="2">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033238069.1"/>
        </source>
    </evidence>
    <sequence length="248" mass="27738" checksum="683D44D63188E770" modified="2020-08-12" version="1">MSDLVGTEEFSAAQLRGWLESLNLPKGGSKAAMAARLNEIPVELRGQGPPAAETCENEREDEAAADQDSTKSERKEKNEKAPQAPGHNNNHGEYRAEVEMLKLQVELLKLQSEREKEGRGENTTPAANNDASVMLLNAAKDMLPTYHGNISGNNDDVTTWIAQFKAIAKVNKLKDEKLLMLLMSKLKNKALVWLHSSPEHMSLPIDQLLNVMEDTFHPKESKLLLRRKFESRSWARQRQSVAGIPHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W642</accession>
    <name>A0A6I8W642_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 10">Homeotic protein female sterile isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10 11 12 13 14 15" type="primary">fs(1)h</name>
    </gene>
    <organism evidence="7 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9 10">MV-25-SWS-2005</strain>
            <tissue evidence="8 9 10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238139.1">
        <property type="nucleotide sequence ID" value="XM_033382248.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238140.1">
        <property type="nucleotide sequence ID" value="XM_033382249.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238141.1">
        <property type="nucleotide sequence ID" value="XM_033382250.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238142.1">
        <property type="nucleotide sequence ID" value="XM_033382251.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238143.1">
        <property type="nucleotide sequence ID" value="XM_033382252.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238144.1">
        <property type="nucleotide sequence ID" value="XM_033382253.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238145.1">
        <property type="nucleotide sequence ID" value="XM_033382254.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238146.1">
        <property type="nucleotide sequence ID" value="XM_033382255.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247576">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W642">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05497">
        <property type="entry name" value="Bromo_Brdt_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05498">
        <property type="entry name" value="Bromo_Brdt_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000003">
        <property type="entry name" value="Bromodomain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.220:FF:000001">
        <property type="entry name" value="bromodomain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000002">
        <property type="entry name" value="Bromodomain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031354">
        <property type="entry name" value="BRD4_CDT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043508">
        <property type="entry name" value="Bromo_Brdt_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043509">
        <property type="entry name" value="Bromo_Brdt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF225">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN BET-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17105">
        <property type="entry name" value="BRD4_CDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="51"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="501"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="6">
        <location>
            <begin position="943"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="145"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="597"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="683"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="823"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="872"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1021"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1238"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1323"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1398"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1463"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1528"/>
            <end position="1547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1623"/>
            <end position="1649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1671"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1718"/>
            <end position="1863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1902"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="599"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="642"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="687"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="708"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="720"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="826"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="872"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="903"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="917"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1021"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1033"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1084"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1323"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1398"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1473"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1528"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1623"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1671"/>
            <end position="1684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1693"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1726"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1758"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1768"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1790"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1815"/>
            <end position="1839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1840"/>
            <end position="1862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1927"/>
            <end position="1944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1945"/>
            <end position="1964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033238139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033238140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033238141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033238143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033238144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033238145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033238146.1"/>
        </source>
    </evidence>
    <sequence length="1985" mass="202511" checksum="90F2B92ED46CA187" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6T0</accession>
    <name>A0A6I8W6T0_DROPS</name>
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        <submittedName>
            <fullName evidence="6 7 8">Eukaryotic translation initiation factor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8" type="primary">LOC6901682</name>
    </gene>
    <organism evidence="5 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7 8">MV-25-SWS-2005</strain>
            <tissue evidence="6 7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239073.1">
        <property type="nucleotide sequence ID" value="XM_033383182.1"/>
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    <dbReference type="RefSeq" id="XP_033239074.1">
        <property type="nucleotide sequence ID" value="XM_033383183.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239075.1">
        <property type="nucleotide sequence ID" value="XM_033383184.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8W6T0"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="eIF1_SUI1"/>
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        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SUI1"/>
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        <property type="entry name" value="SUI1_dom_sf"/>
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        <property type="entry name" value="SUI1_euk"/>
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    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
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        <property type="entry name" value="SUI1"/>
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    </dbReference>
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        <property type="entry name" value="eIF1-like"/>
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        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033239073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033239074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033239075.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="12687" checksum="958277F1F04FA9C0" modified="2020-08-12" version="1">MSKQNLTGGDVNKDNGVERPPKQVHIRIQKGFGYKMQTTVQGLSDEYKLMKIVRAFKKEFTCNVIVTEHPEYGEVIQVQGDQRQRISRWLVRMGLAKSDQVKLHSPDERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6T8</accession>
    <name>A0A6I8W6T8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117184643</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239076.1">
        <property type="nucleotide sequence ID" value="XM_033383185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6T8"/>
    <dbReference type="KEGG" id="dpo:117184643"/>
    <dbReference type="InParanoid" id="A0A6I8W6T8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033239076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12980" checksum="EA3B3D1FA831ADA5" modified="2020-08-12" version="1">MAHEEDHPPNDLVRKISSDSERTGKDLLIGCDANAHHTQWGSKDTNVRDTIKSWKVLGDHFFSDHRYIETILSFEIPKPTTISTPEKPTGKNIAQPWRNYSLLTPLIARTLKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W737</accession>
    <name>A0A6I8W737_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Actin-5C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC26534122</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the actin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239163.1">
        <property type="nucleotide sequence ID" value="XM_033383272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W737"/>
    <dbReference type="KEGG" id="dpo:26534122"/>
    <dbReference type="InParanoid" id="A0A6I8W737"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0558">Oxidation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033239163.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="41808" checksum="5E8B8857DA74201B" modified="2020-08-12" version="1">MCDEEVAALVVDNGSGMCKVGFAGDDAPRAVFPSIVGRPRHQGVMMGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMGKIWHHTFYNELRVAPEEHPILLTEAPLNPKANREKMTQIMFEAFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSMEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITAVAPSTMKIKIVAPPERKYSVWIGESILASLSTFQQIWISKQVYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8Q3</accession>
    <name>A0A6I8W8Q3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">LOW QUALITY PROTEIN: uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26533235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239765.1">
        <property type="nucleotide sequence ID" value="XM_033383874.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26533235"/>
    <dbReference type="InParanoid" id="A0A6I8W8Q3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033239765.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15483" checksum="968069BAB3CAB5A4" modified="2020-08-12" version="1">MNQLDQTTCTKAAAIHTDTHTHTHSEILGATIQQQGRXKEVRFRDRRRRILYVPRSGARWRSSSQLVQHSRGIDDNIHSFDQTADDVVDDQDDFHYALFSSVSTSASFSYSSSSCCCCCFFLALALKPLGQAWAAAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W945</accession>
    <name>A0A6I8W945_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein DDB_G0292186-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239891.1">
        <property type="nucleotide sequence ID" value="XM_033384000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W945"/>
    <dbReference type="KEGG" id="dpo:6900801"/>
    <dbReference type="InParanoid" id="A0A6I8W945"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239891.1"/>
        </source>
    </evidence>
    <sequence length="84" mass="9623" checksum="F326E4AB6A60E5B3" modified="2020-08-12" version="1">MGKGKDMWHTRVTHTTAIEIRHCHCDDGHSKVKRQDLAQPQPQPQSQHQSQSQSQSQPQSQTLMQKQIEGINWILPQTGTNLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W986</accession>
    <name>A0A6I8W986_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239936.1">
        <property type="nucleotide sequence ID" value="XM_033384045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W986"/>
    <dbReference type="KEGG" id="dpo:4812126"/>
    <dbReference type="InParanoid" id="A0A6I8W986"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004011">
        <property type="entry name" value="Gyr_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004019">
        <property type="entry name" value="YLP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15363">
        <property type="entry name" value="POU DOMAIN CLASS 2-ASSOCIATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15363:SF3">
        <property type="entry name" value="POU DOMAIN CLASS 2-ASSOCIATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02756">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02757">
        <property type="entry name" value="YLP"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00713">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026090138" evidence="1">
        <location>
            <begin position="17"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239936.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="59933" checksum="C2E50D6A8F9F0A69" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WB86</accession>
    <name>A0A6I8WB86_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117184855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239804.1">
        <property type="nucleotide sequence ID" value="XM_033383913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WB86"/>
    <dbReference type="KEGG" id="dpo:117184855"/>
    <dbReference type="InParanoid" id="A0A6I8WB86"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF6">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="2">
        <location>
            <begin position="48"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239804.1"/>
        </source>
    </evidence>
    <sequence length="145" mass="16191" checksum="0DA792A8B12641D8" modified="2020-08-12" version="1">MNSRPLGAISQDPSDGEALTPGHLLTGGSLIAPPALRTPDQESISCLRRWRLVSSVRQAFWQRWSREYVLGLQIRGKWHQQQPNLEEGDLVIVAEDNLPPQEWLVGRVIATHAGEDGMVRVVEIRTSTGAVFRRPIHKLAPLPMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBL5</accession>
    <name>A0A6I8WBL5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein sex-lethal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Sxl</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033240800.1">
        <property type="nucleotide sequence ID" value="XM_033384909.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902175"/>
    <dbReference type="InParanoid" id="A0A6I8WBL5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244056">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WBL5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050686">
        <property type="term" value="P:negative regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007530">
        <property type="term" value="P:sex determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12649">
        <property type="entry name" value="RRM1_SXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12376">
        <property type="entry name" value="RRM2_Hu_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000205">
        <property type="entry name" value="Sex lethal, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000383">
        <property type="entry name" value="Sex lethal, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006546">
        <property type="entry name" value="Sxl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01659">
        <property type="entry name" value="sex-lethal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0726">Sexual differentiation</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="119"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="205"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="421"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="477"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="651"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="495"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="546"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="651"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="668"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="688"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033240800.1"/>
        </source>
    </evidence>
    <sequence length="785" mass="85683" checksum="342B59C994FE726D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBM1</accession>
    <name>A0A6I8WBM1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein Smaug</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">smg</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMAUG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240801.1">
        <property type="nucleotide sequence ID" value="XM_033384910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WBM1"/>
    <dbReference type="FunCoup" id="A0A6I8WBM1">
        <property type="interactions" value="1123"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813986"/>
    <dbReference type="InParanoid" id="A0A6I8WBM1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WBM1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030371">
        <property type="term" value="F:translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09557">
        <property type="entry name" value="SAM_Smaug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000076">
        <property type="entry name" value="Smg, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.170">
        <property type="entry name" value="Smaug, PHAT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015327">
        <property type="entry name" value="PHAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037093">
        <property type="entry name" value="PHAT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058599">
        <property type="entry name" value="PHAT_Smg/ZCCHC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050897">
        <property type="entry name" value="SMAUG/VTS1_RNA-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037634">
        <property type="entry name" value="Smaug_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515:SF5">
        <property type="entry name" value="PROTEIN SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09246">
        <property type="entry name" value="PHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26034">
        <property type="entry name" value="PHAT_SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SAM" evidence="11">
        <location>
            <begin position="603"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="345"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="786"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="839"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="937"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="811"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="867"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="950"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="976"/>
            <end position="991"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033240801.1"/>
        </source>
    </evidence>
    <sequence length="1135" mass="124037" checksum="8E52697344911149" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEG2</accession>
    <name>A0A6I8WEG2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14 15">Neprilysin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14 15" type="primary">Nep1</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14 15">MV-25-SWS-2005</strain>
            <tissue evidence="13 14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240957.1">
        <property type="nucleotide sequence ID" value="XM_033385066.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240958.1">
        <property type="nucleotide sequence ID" value="XM_033385067.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240959.1">
        <property type="nucleotide sequence ID" value="XM_033385068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEG2"/>
    <dbReference type="KEGG" id="dpo:4814942"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF241">
        <property type="entry name" value="GH26575P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="11">
        <location>
            <begin position="233"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="10">
        <location>
            <begin position="681"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033240957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033240958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033240959.1"/>
        </source>
    </evidence>
    <sequence length="887" mass="99557" checksum="577518C5867C6355" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEI8</accession>
    <name>A0A6I8WEI8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein beat-IV</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">beat-IV</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241836.1">
        <property type="nucleotide sequence ID" value="XM_033385945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEI8"/>
    <dbReference type="FunCoup" id="A0A6I8WEI8">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800555"/>
    <dbReference type="InParanoid" id="A0A6I8WEI8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261">
        <property type="entry name" value="BEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261:SF14">
        <property type="entry name" value="BEATEN PATH IV, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="212"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241836.1"/>
        </source>
    </evidence>
    <sequence length="444" mass="49948" checksum="0D028FA9D431FDE0" modified="2020-08-12" version="1">MKYPNDTRIIVLICCLLQGLAVGLRMIKVSIPAYKLRGESAFLECQYELNRTHHLGLGGHGLQTHSDHSYGRNHDRPSHAGDFHYPDGAESVDDERPPHRGRMHQGQRQHGQRQRQRDREPIAMGQYRNPPPLTPPEKSPYGHSVYRGSAASGYLGAHVGASTHSGGSISTNGGSMGYMPEFDRTDSFGDSAEEEDEDEEEQAEEGEALYAIKWYKDNEEFYRYVPKARPPKTSYRVDGVRVIEELSDASRVLLRGLTLNSTGLYRCEVSAEAPNFSSVQGEGRMDIVFLPRDGPHIRGQQYQYQIGEYLYLNCTSGKSHPASHLQWFVNEQPILDEHYLYKYNDIVHKHGLITSTLGLQLPLESRHFHDGDMRVKCLASISPVLWKGGKESVLQRRPGIIDNREAMLLVRSAAGHSQNSLLRSLTITIIVARACQWLLGSELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>B5DHW8</accession>
    <accession>A0A6I8UX79</accession>
    <name>B5DHW8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger protein OZF-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6903305</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002132493.1">
        <property type="nucleotide sequence ID" value="XM_002132457.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DHW8"/>
    <dbReference type="SMR" id="B5DHW8"/>
    <dbReference type="GeneID" id="6903305"/>
    <dbReference type="KEGG" id="dpo:6903305"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002678_94_1_1"/>
    <dbReference type="InParanoid" id="B5DHW8"/>
    <dbReference type="OMA" id="CYLRTHK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246912">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DHW8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002132493.1"/>
        </source>
    </evidence>
    <sequence length="412" mass="47788" checksum="4A47E744D53D48D1" modified="2008-10-14" version="1">MAQVCRVCMAPPVSGPFKNIFDEPQNWDTCIADMIAECTGYVVCRGDLLPENICPPCLEDAVSAFSFKKTCEQSHRLYIPVLEEAGGEDICHNLEDDGDWEHSDSGNELTNQFENAKVHGDKDVDHQFKCSLCPKSYTHKSNLNRHKQLHTEERTYKCSDCSKPFRLKCYLRTHKKSHCSKSFKLQSDLKAHTHTHTVDRPYHCSYCPMSFTQKCNLQAHTRTHTGDRPFQCDHCSKSFKQKGHLQEHVRTHTGARRFKCSHCSMSFKYQYELKQHIQCHTEERPYKCTYCSQSYQDLSHLQEHICTPAERLHKCSYCIQSFSDKSKYLIHTRSHTGERPYPCSHCLKSFKGKSNLQVHMRSHTGEHPYKCTHCSKTFRYRNNSYHKHILAHTSEDKLGQEDTIVPSAERGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKJ6</accession>
    <accession>A0A6I8UZB0</accession>
    <name>B5DKJ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein C18orf19 homolog A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6901883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002133418.2">
        <property type="nucleotide sequence ID" value="XM_002133382.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKJ6"/>
    <dbReference type="FunCoup" id="B5DKJ6">
        <property type="interactions" value="452"/>
    </dbReference>
    <dbReference type="GeneID" id="6901883"/>
    <dbReference type="KEGG" id="dpo:6901883"/>
    <dbReference type="eggNOG" id="KOG4082">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084299_1_0_1"/>
    <dbReference type="InParanoid" id="B5DKJ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244289">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DKJ6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045866">
        <property type="entry name" value="FAM210A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009688">
        <property type="entry name" value="FAM210A/B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF1">
        <property type="entry name" value="PROTEIN FAM210A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06916">
        <property type="entry name" value="FAM210A-B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002133418.2"/>
        </source>
    </evidence>
    <sequence length="248" mass="27582" checksum="A2999AF7D8835B16" modified="2016-01-20" version="2">MATMSQLTRLIGHGAQQRTFARFLTHSLTTKSNTRCLFGGSNWSGRNRDLAPPHCTTTPATRYLQSPWLFTRSRSLGHKSTQPTPAPVATNKTDSNASSTTATDLFGDAASKGLFAKFRHMYKQYWYVLIPVHCVTSIGWFGGFYYLSKSGVDVPSLLQYVHLSETIIEKVQSSDMGHYAIAYLCYKVATPLRYALTLGGTTVSIKYLVQRGHIKPIPSKNELIKMYEDKKATRASAKADKAEKDGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>B5DL65</accession>
    <accession>A0A6I8UYU1</accession>
    <name>B5DL65_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Gamma-butyrobetaine dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6902106</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; carnitine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133637.1">
        <property type="nucleotide sequence ID" value="XM_002133601.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DL65"/>
    <dbReference type="SMR" id="B5DL65"/>
    <dbReference type="FunCoup" id="B5DL65">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DL65"/>
    <dbReference type="GeneID" id="6902106"/>
    <dbReference type="KEGG" id="dpo:6902106"/>
    <dbReference type="eggNOG" id="KOG3888">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021859_2_0_1"/>
    <dbReference type="InParanoid" id="B5DL65"/>
    <dbReference type="OMA" id="YEAYDKF"/>
    <dbReference type="UniPathway" id="UPA00118"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244107">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2020.30:FF:000002">
        <property type="entry name" value="Putative gamma-butyrobetaine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000001">
        <property type="entry name" value="Trimethyllysine dioxygenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF33">
        <property type="entry name" value="GAMMA-BUTYROBETAINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0124">Carnitine biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="11">
        <location>
            <begin position="29"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="TauD/TfdA-like" evidence="10">
        <location>
            <begin position="138"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002133637.1"/>
        </source>
    </evidence>
    <sequence length="405" mass="47361" checksum="892F275E563804CC" modified="2008-10-14" version="1">MLSCRHLIGRFLVRRQASTQARLASPGIVEIDEADGQRLKYPQVWLRDNCQCTECFHATTRARKSNWERGPLHVGAQRVSYDEQCQQLQVHWQDEHKSVYELQWLRERDFGDAARQKYLDEVYRPPVQLWGKTQFEEVRREFLYQNVLDRDDVLKAWLEALAVQGFALLKDAPDDENVARRLAERIGYIKRTTYGDVFEVKSKPNAGNYAYLMVPLPLHTDMPYYEYKAGINILHTLVQSESAGGANTLVDGFHVAAEMRRLYPQEFEVLRSVPVNWYDIGHDGDQARPFHSLWRAPVVCLDVDGQVVRINQNTTKRDSRFTVPIEQAVAWYEAYDRFLRLAHDEIVEFKTRPGDVFVFNNLRMLHGRTAYEDSPQNKRHLVGAYVDWDIIYSKLRTLRFPQAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>B5DQS6</accession>
    <accession>A0A6I8V052</accession>
    <name>B5DQS6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134765.1">
        <property type="nucleotide sequence ID" value="XM_002134729.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DQS6"/>
    <dbReference type="GeneID" id="6900370"/>
    <dbReference type="KEGG" id="dpo:6900370"/>
    <dbReference type="HOGENOM" id="CLU_1290163_0_0_1"/>
    <dbReference type="InParanoid" id="B5DQS6"/>
    <dbReference type="OMA" id="RENRLAW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245058">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DQS6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134765.1"/>
        </source>
    </evidence>
    <sequence length="214" mass="23928" checksum="6EAD0997FC78078B" modified="2008-10-14" version="1">MSENNTEYTSEEAARAPMELEPMLNPSDTNSASVAVSPSPSSSSVAVSGGSSSMTSSVLLNFNERAVSTGAERGWTSSEPSVSENTRSSSSLSRHARFPLAMRRNMSDLRRNYTWNHSERRRLIIRRHIQATREHREHRENRLAWDNQVFLGFPDIRASSAASSEDDENEWNRIPETVLARVPTRGNSVSDCSQCSSDPNDEDEGHQDLDEWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>B5DV07</accession>
    <accession>A0A6I8VT43</accession>
    <name>B5DV07_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein windbeutel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">wbl</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234241.1">
        <property type="nucleotide sequence ID" value="XM_033378350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DV07"/>
    <dbReference type="SMR" id="B5DV07"/>
    <dbReference type="FunCoup" id="B5DV07">
        <property type="interactions" value="632"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DV07"/>
    <dbReference type="KEGG" id="dpo:4804608"/>
    <dbReference type="eggNOG" id="ENOG502QSHC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061309_0_0_1"/>
    <dbReference type="InParanoid" id="B5DV07"/>
    <dbReference type="OMA" id="FPYGDKH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244738">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00238">
        <property type="entry name" value="ERp29c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1150.12">
        <property type="entry name" value="Endoplasmic reticulum resident protein 29, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016855">
        <property type="entry name" value="ERp29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011679">
        <property type="entry name" value="ERp29_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036356">
        <property type="entry name" value="ERp29_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012883">
        <property type="entry name" value="ERp29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12211">
        <property type="entry name" value="ENDOPLASMIC RETICULUM PROTEIN ERP29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12211:SF0">
        <property type="entry name" value="ENDOPLASMIC RETICULUM RESIDENT PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07749">
        <property type="entry name" value="ERp29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07912">
        <property type="entry name" value="ERp29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47933">
        <property type="entry name" value="ERP29 C domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERp29 N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum resident protein 29 C-terminal" evidence="1">
        <location>
            <begin position="148"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234241.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29611" checksum="BA9DAB630894D890" modified="2008-10-14" version="1">MMRILVTLLLAAILGRHATWAISCAGCVDLDEINFQNTVERFPYAVVKFDIAFPYGEKHEAFAAFSKAAHKVTGELLVATVGIKDYGELENKALGERFEIDDKKFPAILLFKGSVEQYIRFPSHLDVTLDNLKAFVSSNTPLYIGREGCLKEFNDAIKNYANLPDDAQLSLIEQLETQQEKLSKPEEKLSGKYYVLYMRKINENGYDFLEEETKRLLRLKAGKVTAAKKLELQQKLNILEAFRVHNLTKAAPKKESSKEDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-12-01" modified="2026-01-28" version="143" xmlns="https://uniprot.org/uniprot">
    <accession>P28678</accession>
    <accession>Q294T3</accession>
    <accession>Q56RD3</accession>
    <accession>Q8I154</accession>
    <accession>Q8STC6</accession>
    <name>OPS1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Opsin Rh1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Outer R1-R6 photoreceptor cells opsin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ninaE</name>
        <name type="synonym">Rh1</name>
        <name type="ORF">GA18249</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1992" name="Genetics" volume="132" first="193" last="204">
            <title>Variable rates of evolution among Drosophila opsin genes.</title>
            <authorList>
                <person name="Carulli J.P."/>
                <person name="Hartl D.L."/>
            </authorList>
            <dbReference type="PubMed" id="1398053"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>Apple Hill</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Genome Biol." volume="3" first="RESEARCH0086.1" last="RESEARCH0086.20">
            <title>Assessing the impact of comparative genomic sequence data on the functional annotation of the Drosophila genome.</title>
            <authorList>
                <person name="Bergman C.M."/>
                <person name="Pfeiffer B.D."/>
                <person name="Rincon-Limas D.E."/>
                <person name="Hoskins R.A."/>
                <person name="Gnirke A."/>
                <person name="Mungall C.J."/>
                <person name="Wang A.M."/>
                <person name="Kronmiller B."/>
                <person name="Pacleb J.M."/>
                <person name="Park S."/>
                <person name="Stapleton M."/>
                <person name="Wan K.H."/>
                <person name="George R.A."/>
                <person name="de Jong P.J."/>
                <person name="Botas J."/>
                <person name="Rubin G.M."/>
                <person name="Celniker S.E."/>
            </authorList>
            <dbReference type="PubMed" id="12537575"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>Tucson 14011-0121.4</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Genetics" volume="169" first="1495" last="1507">
            <title>Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda.</title>
            <authorList>
                <person name="Bartolome C."/>
                <person name="Maside X."/>
                <person name="Yi S."/>
                <person name="Grant A.L."/>
                <person name="Charlesworth B."/>
            </authorList>
            <dbReference type="PubMed" id="15545653"/>
            <dbReference type="DOI" id="10.1534/genetics.104.033068"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2002" name="Mol. Biol. Evol." volume="19" first="472" last="488">
            <title>Inferring the history of speciation from multilocus DNA sequence data: the case of Drosophila pseudoobscura and close relatives.</title>
            <authorList>
                <person name="Machado C.A."/>
                <person name="Kliman R.M."/>
                <person name="Markert J.A."/>
                <person name="Hey J."/>
            </authorList>
            <dbReference type="PubMed" id="11919289"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-341</scope>
        <source>
            <strain>Abajo36</strain>
            <strain>AF2</strain>
            <strain>AFC12</strain>
            <strain>AFC3</strain>
            <strain>AFC7</strain>
            <strain>Flagstaff14</strain>
            <strain>Flagstaff16</strain>
            <strain>Flagstaff18</strain>
            <strain>Flagstaff5</strain>
            <strain>Mather10</strain>
            <strain>Mather17</strain>
            <strain>Mather32</strain>
            <strain>Mather48</strain>
            <strain>Mather52</strain>
            <strain>MSH10</strain>
            <strain>MSH21</strain>
            <strain>MSH32</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <absorption>
            <max>480 nm</max>
        </absorption>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Rhabdomere membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text>Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.</text>
    </comment>
    <comment type="miscellaneous">
        <text>Each Drosophila eye is composed of 800 facets or ommatidia. Each ommatidium contains 8 photoreceptor cells (R1-R8), the R1 to R6 cells are outer cells, while R7 and R8 are inner cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="X65877">
        <property type="protein sequence ID" value="CAA46708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY190960">
        <property type="protein sequence ID" value="AAO01119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY754574">
        <property type="protein sequence ID" value="AAX13149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL28881.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450965">
        <property type="protein sequence ID" value="AAM09428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450966">
        <property type="protein sequence ID" value="AAM09429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450967">
        <property type="protein sequence ID" value="AAM09430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450968">
        <property type="protein sequence ID" value="AAM09431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450969">
        <property type="protein sequence ID" value="AAM09432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450970">
        <property type="protein sequence ID" value="AAM09433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450971">
        <property type="protein sequence ID" value="AAM09434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450972">
        <property type="protein sequence ID" value="AAM09435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450973">
        <property type="protein sequence ID" value="AAM09436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450974">
        <property type="protein sequence ID" value="AAM09437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450975">
        <property type="protein sequence ID" value="AAM09438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450976">
        <property type="protein sequence ID" value="AAM09439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450977">
        <property type="protein sequence ID" value="AAM09440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450978">
        <property type="protein sequence ID" value="AAM09441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450979">
        <property type="protein sequence ID" value="AAM09442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450980">
        <property type="protein sequence ID" value="AAM09443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF450981">
        <property type="protein sequence ID" value="AAM09444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S40691">
        <property type="entry name" value="S40691"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001359729.2">
        <property type="nucleotide sequence ID" value="XM_001359692.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P28678"/>
    <dbReference type="SMR" id="P28678"/>
    <dbReference type="DIP" id="DIP-769N"/>
    <dbReference type="FunCoup" id="P28678">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="46245.P28678"/>
    <dbReference type="GlyCosmos" id="P28678">
        <property type="glycosylation" value="2 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0286275">
        <property type="protein sequence ID" value="FBpp0284713"/>
        <property type="gene ID" value="FBgn0012733"/>
    </dbReference>
    <dbReference type="GeneID" id="4802902"/>
    <dbReference type="KEGG" id="dpo:4802902"/>
    <dbReference type="CTD" id="42367"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009579_3_0_1"/>
    <dbReference type="InParanoid" id="P28678"/>
    <dbReference type="OMA" id="WKMAKIV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0012733">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033583">
        <property type="term" value="C:rhabdomere membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008020">
        <property type="term" value="F:G protein-coupled photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15079">
        <property type="entry name" value="7tmA_photoreceptors_insect"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000044">
        <property type="entry name" value="Opsin, ultraviolet-sensitive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001735">
        <property type="entry name" value="Opsin_RH1/RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00576">
        <property type="entry name" value="OPSINRH1RH2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0966">Cell projection</keyword>
    <keyword id="KW-0157">Chromophore</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0297">G-protein coupled receptor</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0600">Photoreceptor protein</keyword>
    <keyword id="KW-0675">Receptor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0681">Retinal protein</keyword>
    <keyword id="KW-0716">Sensory transduction</keyword>
    <keyword id="KW-0807">Transducer</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0844">Vision</keyword>
    <feature type="chain" id="PRO_0000197623" description="Opsin Rh1">
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            <begin position="1"/>
            <end position="373"/>
        </location>
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    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=1" evidence="2">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
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    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=2" evidence="2">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=3" evidence="2">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=4" evidence="2">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="184"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=5" evidence="2">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="241"/>
            <end position="276"/>
        </location>
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    <feature type="transmembrane region" description="Helical; Name=6" evidence="2">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=7" evidence="2">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="332"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="358"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(retinylidene)lysine">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="123"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AAX13149." evidence="5" ref="3">
        <original>MD</original>
        <variation>CC</variation>
        <location>
            <begin position="1"/>
            <end position="2"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA46708." evidence="5" ref="1">
        <original>H</original>
        <variation>Q</variation>
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA46708." evidence="5" ref="1">
        <original>L</original>
        <variation>P</variation>
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA46708." evidence="5" ref="1">
        <original>N</original>
        <variation>D</variation>
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA46708." evidence="5" ref="1">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA46708." evidence="5" ref="1">
        <original>C</original>
        <variation>CC</variation>
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA46708." evidence="5" ref="1">
        <original>N</original>
        <variation>T</variation>
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P06002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="373" mass="41524" checksum="80E580372EC4EABB" modified="2008-12-16" version="4">MDSFAAVATQLGPHFAALSNGSVVDKVTPDMAHLISPYWNQFPAMDPIWAKILTAYMIIIGMISWCGNGVVIYIFATTKSLRTPANLLVINLAISDFGIMITNTPMMGINLYFETWVLGPMMCDIYAGLGSAFGCSSIWSMCMISLDRYQVIVKGMAGRPMTIPLALGKIAYIWFMSSIWCLAPVFGWSRYVPEGNLTSCGIDYLERDWNPRSYLIFYSIFVYYIPLFLICYSYWFIIAAVSAHEKAMREQAKKMNVKSLRSSEDADKSAEGKLAKVALVTISLWFMAWTPYLVINCMGLFKFEGLTPLNTIWGACFAKSAACYNPIVYGISHPKYRLALKEKCPCCVFGKVDDGKSSEAQSQATNSEAESKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q24618</accession>
    <accession>Q28ZQ1</accession>
    <accession>Q8STA2</accession>
    <name>EXU1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Maternal protein exuperantia-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">exu1</name>
        <name type="ORF">GA21461</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1994" name="Genetics" volume="137" first="521" last="530">
            <title>Components acting in localization of bicoid mRNA are conserved among Drosophila species.</title>
            <authorList>
                <person name="Luk S.K.-S."/>
                <person name="Kilpatrick M."/>
                <person name="Kerr K."/>
                <person name="Macdonald P.M."/>
            </authorList>
            <dbReference type="PubMed" id="8070663"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Proc. Natl. Acad. Sci. U.S.A." volume="100" first="8319" last="8324">
            <title>Evolutionary genomics of inversions in Drosophila pseudoobscura: evidence for epistasis.</title>
            <authorList>
                <person name="Schaeffer S.W."/>
                <person name="Goetting-Minesky M.P."/>
                <person name="Kovacevic M."/>
                <person name="Peoples J.R."/>
                <person name="Graybill J.L."/>
                <person name="Miller J.M."/>
                <person name="Kim K."/>
                <person name="Nelson J.G."/>
                <person name="Anderson W.W."/>
            </authorList>
            <dbReference type="PubMed" id="12824467"/>
            <dbReference type="DOI" id="10.1073/pnas.1432900100"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-124</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Ensures the proper localization of the mRNA of the bicoid gene to the anterior regions of the oocyte thus playing a fundamental role in the establishment of the polarity of the oocyte. May bind the bcd mRNA.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL25562" version="1"/>
        </conflict>
    </comment>
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        <property type="protein sequence ID" value="AAA28524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL25562.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476528">
        <property type="protein sequence ID" value="AAL91372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476529">
        <property type="protein sequence ID" value="AAL91373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476530">
        <property type="protein sequence ID" value="AAL91374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476531">
        <property type="protein sequence ID" value="AAL91375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAL91376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAL91377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAL91378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AF476535">
        <property type="protein sequence ID" value="AAL91379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AF476536">
        <property type="protein sequence ID" value="AAL91380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAL91381.1"/>
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        <property type="protein sequence ID" value="AAL91382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAL91383.1"/>
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        <property type="protein sequence ID" value="AAL91384.1"/>
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        <property type="protein sequence ID" value="AAL91385.1"/>
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        <property type="protein sequence ID" value="AAL91386.1"/>
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        <property type="protein sequence ID" value="AAL91388.1"/>
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        <property type="protein sequence ID" value="AAL91389.1"/>
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        <property type="protein sequence ID" value="AAL91390.1"/>
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        <property type="protein sequence ID" value="AAL91391.1"/>
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        <property type="protein sequence ID" value="AAL91392.1"/>
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        <property type="protein sequence ID" value="AAL91396.1"/>
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        <property type="protein sequence ID" value="AAL91424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AF476581">
        <property type="protein sequence ID" value="AAL91425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476582">
        <property type="protein sequence ID" value="AAL91426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476583">
        <property type="protein sequence ID" value="AAL91427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476584">
        <property type="protein sequence ID" value="AAL91428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476585">
        <property type="protein sequence ID" value="AAL91429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476586">
        <property type="protein sequence ID" value="AAL91430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476587">
        <property type="protein sequence ID" value="AAL91431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476588">
        <property type="protein sequence ID" value="AAL91432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476589">
        <property type="protein sequence ID" value="AAL91433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476590">
        <property type="protein sequence ID" value="AAL91434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476591">
        <property type="protein sequence ID" value="AAL91435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476592">
        <property type="protein sequence ID" value="AAL91436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476593">
        <property type="protein sequence ID" value="AAL91437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476594">
        <property type="protein sequence ID" value="AAL91438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476595">
        <property type="protein sequence ID" value="AAL91439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476596">
        <property type="protein sequence ID" value="AAL91440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476597">
        <property type="protein sequence ID" value="AAL91441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476598">
        <property type="protein sequence ID" value="AAL91442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476599">
        <property type="protein sequence ID" value="AAL91443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476600">
        <property type="protein sequence ID" value="AAL91444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476601">
        <property type="protein sequence ID" value="AAL91445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476602">
        <property type="protein sequence ID" value="AAL91446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476603">
        <property type="protein sequence ID" value="AAL91447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476604">
        <property type="protein sequence ID" value="AAL91448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476605">
        <property type="protein sequence ID" value="AAL91449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476606">
        <property type="protein sequence ID" value="AAL91450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476607">
        <property type="protein sequence ID" value="AAL91451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476608">
        <property type="protein sequence ID" value="AAL91452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476609">
        <property type="protein sequence ID" value="AAL91453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476610">
        <property type="protein sequence ID" value="AAL91454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476611">
        <property type="protein sequence ID" value="AAL91455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476612">
        <property type="protein sequence ID" value="AAL91456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476613">
        <property type="protein sequence ID" value="AAL91457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AF476614">
        <property type="protein sequence ID" value="AAL91458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AF476615">
        <property type="protein sequence ID" value="AAL91459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AF476616">
        <property type="protein sequence ID" value="AAL91460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AF476617">
        <property type="protein sequence ID" value="AAL91461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AF476618">
        <property type="protein sequence ID" value="AAL91462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AF476619">
        <property type="protein sequence ID" value="AAL91463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476620">
        <property type="protein sequence ID" value="AAL91464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476621">
        <property type="protein sequence ID" value="AAL91465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476622">
        <property type="protein sequence ID" value="AAL91466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476623">
        <property type="protein sequence ID" value="AAL91467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476624">
        <property type="protein sequence ID" value="AAL91468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476625">
        <property type="protein sequence ID" value="AAL91469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476626">
        <property type="protein sequence ID" value="AAL91470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476627">
        <property type="protein sequence ID" value="AAL91471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476628">
        <property type="protein sequence ID" value="AAL91472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476629">
        <property type="protein sequence ID" value="AAL91473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476630">
        <property type="protein sequence ID" value="AAL91474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476631">
        <property type="protein sequence ID" value="AAL91475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF476632">
        <property type="protein sequence ID" value="AAL91476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S47890">
        <property type="entry name" value="S47890"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q24618"/>
    <dbReference type="SMR" id="Q24618"/>
    <dbReference type="FunCoup" id="Q24618">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q24618"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0279500">
        <property type="protein sequence ID" value="FBpp0277938"/>
        <property type="gene ID" value="FBgn0243576"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0367479">
        <property type="protein sequence ID" value="FBpp0330423"/>
        <property type="gene ID" value="FBgn0243576"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804418"/>
    <dbReference type="eggNOG" id="ENOG502QVAD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034404_1_0_1"/>
    <dbReference type="InParanoid" id="Q24618"/>
    <dbReference type="OMA" id="NWLEMLV"/>
    <dbReference type="PhylomeDB" id="Q24618"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243576">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045450">
        <property type="term" value="P:bicoid mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037998">
        <property type="entry name" value="Exu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054362">
        <property type="entry name" value="Exu_RNase_H-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040941">
        <property type="entry name" value="SAM_Exu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12384">
        <property type="entry name" value="MATERNAL PROTEIN EXUPERANTIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12384:SF2">
        <property type="entry name" value="MATERNAL PROTEIN EXUPERANTIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22123">
        <property type="entry name" value="Exu_RNase_H_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18609">
        <property type="entry name" value="SAM_Exu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000087143" description="Maternal protein exuperantia-1">
        <location>
            <begin position="1"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="377"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="398"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA28524." evidence="3" ref="1">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA28524." evidence="3" ref="1">
        <original>T</original>
        <variation>S</variation>
        <location>
            <position position="424"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA28524." evidence="3" ref="1">
        <original>G</original>
        <variation>S</variation>
        <location>
            <position position="458"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA28524." evidence="3" ref="1">
        <original>E</original>
        <variation>ESL</variation>
        <location>
            <position position="477"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA28524." evidence="3" ref="1">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="495" mass="55039" checksum="D56DCDB8F12C4129" modified="2006-05-16" version="2">MVAEISKDCVTIAAADQCVDMKEELPAGNYILVGVDVDTTGRRLIDEIVQLAAYTPKDNFQQYIMPYMNLNPAARQRHQIRVISIGFYRMLKSMQTYKIIKSKSEVAALMDFLNWLEMLVAKQPSTDGIVMLYHEERKFIPYMVLEALKKYGLIERFNRTVKSFVNTFNMAKASLGDANLKNCGLRKLSLLLAKSNDESANKENEPENVNRNGSSNDKCHKNGMNQEHDFFEGSANVRAKMVYEVALQLTNSDRTSEPESSEELVNLFNAVKPFAKLVSSDIMELQTQNENMGRQNSFRPVFLNYFRTTLYHRVRAVKFRIVLAENGFTLDSLKAIWTEKRKEGLELALTNIDTLKTEEKTELVELLDSFFDPSKATIKPSFKPNGNGPRRRIRANGAASSKNGAMSSRSTSTEFGAGGDKSQTEGLSAPVPDSTTKSPSPGKTGGRPHRKRNNTRNGFGSAKGPKKAETLNIAAPEPPQSPVAVSTPVAIAATN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-04-20" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q292N4</accession>
    <name>LPHN_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Latrophilin Cirl</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">Cirl</name>
        <name type="ORF">GA21229</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Forms a heterodimer, consisting of a large extracellular region non-covalently linked to a seven-transmembrane moiety.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="2 3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="2">Proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL24828.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q292N4"/>
    <dbReference type="SMR" id="Q292N4"/>
    <dbReference type="FunCoup" id="Q292N4">
        <property type="interactions" value="578"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q292N4"/>
    <dbReference type="GlyCosmos" id="Q292N4">
        <property type="glycosylation" value="8 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4193">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003272_0_0_1"/>
    <dbReference type="InParanoid" id="Q292N4"/>
    <dbReference type="OMA" id="NMRVFIY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22830">
        <property type="entry name" value="Gal_Rha_Lectin_dCirl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000001">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000322">
        <property type="entry name" value="latrophilin Cirl isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.610:FF:000006">
        <property type="entry name" value="latrophilin Cirl isoform X2"/>
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        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
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        <property type="entry name" value="GAIN_dom_sf"/>
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    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="GPCR_2_secretin-like"/>
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    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF475">
        <property type="entry name" value="LATROPHILIN CIRL"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
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    <keyword id="KW-1003">Cell membrane</keyword>
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    <keyword id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword id="KW-0597">Phosphoprotein</keyword>
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    <keyword id="KW-0807">Transducer</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
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            <begin position="1"/>
            <end position="1693"/>
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    <feature type="topological domain" description="Extracellular" evidence="3">
        <location>
            <begin position="1"/>
            <end position="774"/>
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    <feature type="transmembrane region" description="Helical; Name=1" evidence="3">
        <location>
            <begin position="775"/>
            <end position="795"/>
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            <begin position="796"/>
            <end position="808"/>
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            <begin position="809"/>
            <end position="829"/>
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    <feature type="topological domain" description="Extracellular" evidence="3">
        <location>
            <begin position="830"/>
            <end position="835"/>
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    <feature type="transmembrane region" description="Helical; Name=3" evidence="3">
        <location>
            <begin position="836"/>
            <end position="856"/>
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    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="3">
        <location>
            <begin position="857"/>
            <end position="882"/>
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    </feature>
    <feature type="transmembrane region" description="Helical; Name=4" evidence="3">
        <location>
            <begin position="883"/>
            <end position="903"/>
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    </feature>
    <feature type="topological domain" description="Extracellular" evidence="3">
        <location>
            <begin position="904"/>
            <end position="927"/>
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    <feature type="transmembrane region" description="Helical; Name=5" evidence="3">
        <location>
            <begin position="928"/>
            <end position="948"/>
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    <feature type="topological domain" description="Cytoplasmic" evidence="3">
        <location>
            <begin position="949"/>
            <end position="975"/>
        </location>
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    <feature type="transmembrane region" description="Helical; Name=6" evidence="3">
        <location>
            <begin position="976"/>
            <end position="996"/>
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    </feature>
    <feature type="topological domain" description="Extracellular" evidence="3">
        <location>
            <begin position="997"/>
            <end position="1003"/>
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    <feature type="transmembrane region" description="Helical; Name=7" evidence="3">
        <location>
            <begin position="1004"/>
            <end position="1024"/>
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    <feature type="topological domain" description="Cytoplasmic" evidence="3">
        <location>
            <begin position="1025"/>
            <end position="1693"/>
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        <location>
            <begin position="30"/>
            <end position="119"/>
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    </feature>
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            <end position="761"/>
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            <begin position="190"/>
            <end position="309"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
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        <location>
            <begin position="716"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="1089"/>
            <end position="1109"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
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    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="1450"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1596"/>
            <end position="1678"/>
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        <location>
            <begin position="259"/>
            <end position="269"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="287"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
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            <begin position="390"/>
            <end position="399"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1307"/>
            <end position="1323"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
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            <end position="1357"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1464"/>
            <end position="1475"/>
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        <location>
            <begin position="1485"/>
            <end position="1498"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1508"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1640"/>
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        <location>
            <begin position="748"/>
            <end position="749"/>
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            <position position="1165"/>
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    <feature type="modified residue" description="Phosphoserine" evidence="1">
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            <position position="1256"/>
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            <position position="1263"/>
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            <position position="1324"/>
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        <location>
            <position position="1325"/>
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        <location>
            <position position="147"/>
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        <location>
            <position position="260"/>
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        <location>
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    </feature>
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        <location>
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        <location>
            <position position="405"/>
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        <location>
            <position position="662"/>
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        <location>
            <position position="710"/>
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        <location>
            <position position="737"/>
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        <location>
            <begin position="731"/>
            <end position="745"/>
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    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A1Z7G7"/>
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    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O88923"/>
        </source>
    </evidence>
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    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00098"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00260"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="EAL24828.2"/>
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    <sequence length="1693" mass="185677" checksum="1A059ECAD209313A" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D09</accession>
    <accession>A0A6I8UC21</accession>
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            <fullName evidence="4">Phosrestin-1</fullName>
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            <fullName evidence="5">Phosrestin I</fullName>
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        <name evidence="11" type="primary">Arr2</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
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            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="11">MV-25-SWS-2005</strain>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:rhabdomere"/>
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        <property type="term" value="F:G protein-coupled receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:negative regulation of phospholipase C-activating phototransduction signaling pathway"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <location>
            <begin position="192"/>
            <end position="350"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00078972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00080322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353104.1"/>
        </source>
    </evidence>
    <sequence length="401" mass="45028" checksum="880FD268C1D7C968" modified="2006-04-04" version="1">MVVSVKVFKKATPNGKVTFYLGRRDFIDHLDYCDPVDGVIVVEPEYLKNRKVFGQLATTYRYGREEDEVMGVKFSKELILSREQIVPMTNPNMEMTPMQEKLVRKLGSNAHPFTFHFPPNSPSSVTLQQEGDDNGKPLGVEYTIRAFVGDSEDDRQHKRSMVSLVIKKLQYAPLNRGQRLPSSLVSKGFTFSNGKISLEVTLDREIYYHGEKTAATVQVSNNSKKSVKSIKCFIVQHTEITMVNAQFSKHVAQLETKEGCPITPGANLTKTFYLIPLAANNKDRHGIALDGHLKDEDVNLASSTMVQEGKNTGDACGIVISYSVRIKLNCGTLGGEMQTDVPFKLLQPAPGTIEKKRSNAMKKMKSIEQHRNVKGYYQDDDDNIVFEDFAKMRMNNVNMAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D73</accession>
    <accession>A0A6I8VMM0</accession>
    <name>Q29D73_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Teh3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Teh3</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015044101.1">
        <property type="nucleotide sequence ID" value="XM_015188615.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D73"/>
    <dbReference type="FunCoup" id="Q29D73">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29D73"/>
    <dbReference type="GeneID" id="4812651"/>
    <dbReference type="KEGG" id="dpo:4812651"/>
    <dbReference type="eggNOG" id="ENOG502QVC7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_615831_0_0_1"/>
    <dbReference type="InParanoid" id="Q29D73"/>
    <dbReference type="OMA" id="CQMTAVL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075073">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017080">
        <property type="term" value="F:sodium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031578">
        <property type="entry name" value="TipE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12335:SF7">
        <property type="entry name" value="RT03134P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12335">
        <property type="entry name" value="TIPE PROTEIN TEMPERATURE-INDUCED PARALYTIC E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015044101.1"/>
        </source>
    </evidence>
    <sequence length="448" mass="49227" checksum="D16FF0AEE1A4B7CF" modified="2006-04-04" version="1">MGKKKVAEEDLVVPPQDTRICGTICICQMTLVLSSVALVYLTVAIYMPSTRAFKSGIDPTPVMCTTTRALNKDNCEWGSCGEWCLSKTSGACIQIYVNLRSNGSNLIFQNCTNSANKTCYGIDQDRADKARCINDECKNLTGTFNCTAGQCLNITDAFECIFHNSDAPVKCSGRRGKINCMDISGLYSCSRGTCRKIRTPYNCDRRCVDIPTRNKNVVVLSGDKVYLSQCQNAINAETLEEVWNESSENVAMTSCYFIKNTSDRVDAVDCINGSTLEHNMLSDLTNFTYLSHLHVSVATPVPEIAPPDVDLTISNESKLMINLEGCVNTLMDECKEFLKDFGRDGSDHNARARFPCFYSPGKKDIVVARFDLEVTYRQFVFASVVPSVLFVVSCSILLMCQKTVYVGDDAKMRFKGCVDTETILNKNNVGAPTNGDMGGGGGDEVMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E35</accession>
    <accession>A0A6I8UB74</accession>
    <name>Q29E35_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein C3H8.02</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4812071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352728.1">
        <property type="nucleotide sequence ID" value="XM_001352692.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E35"/>
    <dbReference type="SMR" id="Q29E35"/>
    <dbReference type="FunCoup" id="Q29E35">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E35"/>
    <dbReference type="GeneID" id="4812071"/>
    <dbReference type="KEGG" id="dpo:4812071"/>
    <dbReference type="eggNOG" id="KOG1471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014001_9_3_1"/>
    <dbReference type="InParanoid" id="Q29E35"/>
    <dbReference type="OMA" id="WRVEYGV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076953">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29E35">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000028">
        <property type="entry name" value="GH16843p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052432">
        <property type="entry name" value="PITP/CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590:SF4">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001352728.1"/>
        </source>
    </evidence>
    <sequence length="223" mass="25969" checksum="D9E2AEEB499A8ABC" modified="2006-04-04" version="1">MSSEELAPINEQDLKDLKERMKLIVEADPKQYHNDFSLRRYLRAFKTTDDAFQAILKTNKWRETYGVEKLGEMDRSQLENKARLLRHRDCIGRPVIYIPAKNHGSSTRDIDELTRFIVYNLEEACKKCFEEVTDRLCIVFDLAEFSTSCMDYQLVQNLIWLLGKHFPERLGVCLILNSPGLFSTVWPAIRVLLDDNTAKKVKFVSDDVDLCQYLIPDILPTDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EF4</accession>
    <accession>A0A6I8UB17</accession>
    <name>Q29EF4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4812235</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352609.1">
        <property type="nucleotide sequence ID" value="XM_001352573.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EF4"/>
    <dbReference type="SMR" id="Q29EF4"/>
    <dbReference type="FunCoup" id="Q29EF4">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EF4"/>
    <dbReference type="GeneID" id="4812235"/>
    <dbReference type="KEGG" id="dpo:4812235"/>
    <dbReference type="eggNOG" id="ENOG502QWJA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1176513_0_0_1"/>
    <dbReference type="InParanoid" id="Q29EF4"/>
    <dbReference type="OMA" id="KVSNCYQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077469">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EF4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005958">
        <property type="term" value="C:DNA-dependent protein kinase-DNA ligase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010165">
        <property type="term" value="P:response to X-ray"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.210.10">
        <property type="entry name" value="DNA double-strand break repair and VJ recombination XRCC4, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010585">
        <property type="entry name" value="DNA_repair_prot_XRCC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038051">
        <property type="entry name" value="XRCC4-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053961">
        <property type="entry name" value="XRCC4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28559">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28559:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06632">
        <property type="entry name" value="XRCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58022">
        <property type="entry name" value="XRCC4, C-terminal oligomerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XRCC4 N-terminal" evidence="6">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352609.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="27372" checksum="024C4E0495D17435" modified="2006-04-04" version="1">MTCFVVKLLQRTQLSHSQVDVKPFIYARCNWLDDEAEFHFLSTFNNQNYRGSLKYTEIRSAANDLDLEYEAFLAECKKALTTHMGLPGFDYEISQEDEQPQAFKLYKCAGYETLYLDIPLKKVSNCYQLLDGAIDLGQQQATPAPSPDIEQKTKVMLEEYEKYVTASKQKETEMLKKFILLINSKKAHIRDLKRQLEERPERHSKQRKIISDSEDTDKDEEDFGAATQAMNIEKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FI1</accession>
    <accession>A0A6I8UEQ1</accession>
    <name>Q29FI1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Mdr65</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001354221.2">
        <property type="nucleotide sequence ID" value="XM_001354185.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="Q29FI1">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FI1"/>
    <dbReference type="GeneID" id="4814152"/>
    <dbReference type="KEGG" id="dpo:4814152"/>
    <dbReference type="eggNOG" id="KOG0055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_17_2_1"/>
    <dbReference type="InParanoid" id="Q29FI1"/>
    <dbReference type="OMA" id="LTMEDTI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070195">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FI1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097254">
        <property type="term" value="P:renal tubular secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017085">
        <property type="term" value="P:response to insecticide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000479">
        <property type="entry name" value="Multidrug resistance protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000153">
        <property type="entry name" value="multidrug resistance protein homolog 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001370">
        <property type="entry name" value="p-GlycoProtein related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF27">
        <property type="entry name" value="ATP-DEPENDENT TRANSLOCASE ABCB1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001354221.2"/>
        </source>
    </evidence>
    <sequence length="1309" mass="143929" checksum="6E798B564D31A48B" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HG3</accession>
    <accession>A0A6I8UFP1</accession>
    <name>Q29HG3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">MED18</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354740.1">
        <property type="nucleotide sequence ID" value="XM_001354704.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HG3"/>
    <dbReference type="SMR" id="Q29HG3"/>
    <dbReference type="FunCoup" id="Q29HG3">
        <property type="interactions" value="1256"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HG3"/>
    <dbReference type="GeneID" id="4814890"/>
    <dbReference type="KEGG" id="dpo:4814890"/>
    <dbReference type="CTD" id="54797"/>
    <dbReference type="eggNOG" id="KOG3264">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084570_0_0_1"/>
    <dbReference type="InParanoid" id="Q29HG3"/>
    <dbReference type="OMA" id="ARGYMFR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073295">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.320.10:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001354740.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="24724" checksum="A5679B841C7058C2" modified="2006-04-04" version="1">MSSVSSARESLSHALNNRFLPNLEYLLQGSILDSAVEHLMHRLKGLCDNVDTSPETFHDLEVCMSLRQPNSNQPLLLRVRRALGRDAPFQMRYLGQPEVDQRRPTLVRSCMDCACTNGILEFLTEMGFRLEFEYIAKGYMFRKGRMKITVSKLIKIIPGKQQDMANEPISQSYIVELSVVAPTGQENVGEEMRVFAEQLKPLVQLDKIDYKRLGSMP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JT7</accession>
    <name>PDE6D_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Probable cGMP 3',5'-cyclic phosphodiesterase subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">PrBP</name>
        <name type="ORF">GA21678</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Interacts with Pde6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PDE6D/unc-119 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379062">
        <property type="protein sequence ID" value="EAL32874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001355815.1">
        <property type="nucleotide sequence ID" value="XM_001355779.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JT7"/>
    <dbReference type="SMR" id="Q29JT7"/>
    <dbReference type="FunCoup" id="Q29JT7">
        <property type="interactions" value="1581"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JT7"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0368600">
        <property type="protein sequence ID" value="FBpp0331333"/>
        <property type="gene ID" value="FBgn0081663"/>
    </dbReference>
    <dbReference type="GeneID" id="4816170"/>
    <dbReference type="KEGG" id="dpo:4816170"/>
    <dbReference type="CTD" id="34196"/>
    <dbReference type="eggNOG" id="KOG4038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119682_0_0_1"/>
    <dbReference type="InParanoid" id="Q29JT7"/>
    <dbReference type="OMA" id="STNTWQN"/>
    <dbReference type="PhylomeDB" id="Q29JT7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081663">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050953">
        <property type="term" value="P:sensory perception of light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.40:FF:000002">
        <property type="entry name" value="Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.40">
        <property type="entry name" value="GMP phosphodiesterase, delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037036">
        <property type="entry name" value="PDED_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017287">
        <property type="entry name" value="Rhodop-sen_GMP-Pdiesterase_dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12976">
        <property type="entry name" value="RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE DELTA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12976:SF0">
        <property type="entry name" value="RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037825">
        <property type="entry name" value="GMP-Pdiesterase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0140">cGMP</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000363678" description="Probable cGMP 3',5'-cyclic phosphodiesterase subunit delta">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9VLJ0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000312" key="3">
        <source>
            <dbReference type="EMBL" id="EAL32874.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="17320" checksum="0A3337CA52DC7593" modified="2006-04-04" version="1">MGSDDQSAADKIQKGFQINYMILRDADSGKIIWQENKDFSAPDVEHEARVPVKILDMRAVSREINFSTVESMENFRLDQKVLFKGRIMEEWFFEMGFVGASTTNTWQSTIEAAPESQMMPAKVLNGNVTIQTSFYDNETLITKSVVRLYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q29K70</accession>
    <accession>A0A6I8UJ02</accession>
    <name>Q29K70_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 8">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CAH1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 8"/>
    <dbReference type="RefSeq" id="XP_001356245.1">
        <property type="nucleotide sequence ID" value="XM_001356209.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29K70"/>
    <dbReference type="SMR" id="Q29K70"/>
    <dbReference type="FunCoup" id="Q29K70">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29K70"/>
    <dbReference type="GeneID" id="4816526"/>
    <dbReference type="KEGG" id="dpo:4816526"/>
    <dbReference type="eggNOG" id="KOG0382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039326_2_1_1"/>
    <dbReference type="InParanoid" id="Q29K70"/>
    <dbReference type="OMA" id="INPHWKV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080602">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29K70">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF141">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356245.1"/>
        </source>
    </evidence>
    <sequence length="270" mass="29944" checksum="68F9AC606B416F09" modified="2006-04-04" version="1">MSHHWGYTEENGPAHWAKDYPQASGHRQSPVDITPSSAKKGSELKASALKWKYVPEHTKSLVNPGYCWRVDVNGTESQLTGGPLGDQIFKLEQFHCHWGCTDSKGSEHTVDGVSYAGELHLVHWNTTKYKSFGEAAAAPDGLAVLGVFLQAGKHHAELDKVSSLLQFVLHKGDRVTLPNGCDPGQLLPDVHTYWTYEGSLTTPPCSECVIWIVFKTPIEVSDDQLNAMRNLNAYDVKEECPCNEFNGKVINNFRPPLPLGKRELREIGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LB9</accession>
    <accession>A0A6I8UL05</accession>
    <name>Q29LB9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4817672</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001357060.2">
        <property type="nucleotide sequence ID" value="XM_001357024.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LB9"/>
    <dbReference type="SMR" id="Q29LB9"/>
    <dbReference type="FunCoup" id="Q29LB9">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LB9"/>
    <dbReference type="GeneID" id="4817672"/>
    <dbReference type="KEGG" id="dpo:4817672"/>
    <dbReference type="eggNOG" id="KOG1163">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019279_2_3_1"/>
    <dbReference type="InParanoid" id="Q29LB9"/>
    <dbReference type="OMA" id="LMRLECV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080092">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000596">
        <property type="entry name" value="CK1 family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="34"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001357060.2"/>
        </source>
    </evidence>
    <sequence length="374" mass="43068" checksum="0B836F48E66E89CE" modified="2008-10-14" version="2">MKSQTQAPMEDSTPSGRNRKPSLHLDNQLVGGKYRVVKRIGSGSFGDIYLGVCLKDASEVAIKVERNEAKYPQLAYEAKVYQKLAKNPGFPTLKHFGCEQHFKAMVIELLGPSLEELFSTCKRRFSLKTVLMVADQLLSRLENLHTIGFIHRDIKPDNFLMGTGKQAHKLFLIDFGLSKRYLDAESLLHIPFRKDRNLTGTVRYASVNAQKGIEQSRRDDLESMCHCLMYFNLGKLPWQGITAANKKQKYEMILEKKSGTKIEELCKAFPSEFAVFMKYVRSMRFNEDPDYNYMRQLFRLLSRSLNYQYDNVFDWSLLKQQKLERQRERDQERARGITGARLSLAKKGDHLPLGSGRKFSHCAVGDRSTTCIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q29M45</accession>
    <accession>A0A6I8UK21</accession>
    <name>Q29M45_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GATOR complex protein Wdr59</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Wdr59</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat WDR59 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356784.2">
        <property type="nucleotide sequence ID" value="XM_001356748.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29M45"/>
    <dbReference type="FunCoup" id="Q29M45">
        <property type="interactions" value="854"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29M45"/>
    <dbReference type="GeneID" id="4817259"/>
    <dbReference type="KEGG" id="dpo:4817259"/>
    <dbReference type="eggNOG" id="KOG0309">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009370_0_0_1"/>
    <dbReference type="InParanoid" id="Q29M45"/>
    <dbReference type="OMA" id="HRRETCL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078374">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29M45">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16692">
        <property type="entry name" value="mRING-H2-C3H3C2_WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001753">
        <property type="entry name" value="GATOR complex protein Wdr59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039456">
        <property type="entry name" value="WDR59_mRING-H2-C3H3C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001356784.2"/>
        </source>
    </evidence>
    <sequence length="986" mass="109635" checksum="DA8A1971B267B82B" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MH4</accession>
    <accession>A0A6I8UJT7</accession>
    <name>Q29MH4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Caltractin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4817140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356655.1">
        <property type="nucleotide sequence ID" value="XM_001356619.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MH4"/>
    <dbReference type="SMR" id="Q29MH4"/>
    <dbReference type="FunCoup" id="Q29MH4">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MH4"/>
    <dbReference type="GeneID" id="4817140"/>
    <dbReference type="KEGG" id="dpo:4817140"/>
    <dbReference type="eggNOG" id="KOG0028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_18_2_1"/>
    <dbReference type="InParanoid" id="Q29MH4"/>
    <dbReference type="OMA" id="MNNEQIN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076503">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MH4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000527">
        <property type="entry name" value="Calmodulin-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="36"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="109"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356655.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="20705" checksum="A6F418D8A083A9E4" modified="2006-04-04" version="1">MDSVVSTATLMQSTASLSKGKKAKKVRLPTFDLSLEQKKDIKKAFDLFDTECTGFIEVKELRVAIRALGFEPKKEEIKSMMDEIDKDKTGLIAFNDFLYLMRRKMAEKDTMEDMLKAFAFFDDDRTNRCTFDNLKRVAKELGEDLNDEELQEMIDEANTSGDGEVTREEFLAFTKKTHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZL8</accession>
    <accession>A0A6I8UDD2</accession>
    <name>Q2LZL8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Probable Golgi SNAP receptor complex member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Membrin</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 11">Involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
            <location evidence="9">cis-Golgi network membrane</location>
            <topology evidence="9">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the GOSR2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353755.1">
        <property type="nucleotide sequence ID" value="XM_001353719.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZL8"/>
    <dbReference type="FunCoup" id="Q2LZL8">
        <property type="interactions" value="2439"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZL8"/>
    <dbReference type="GeneID" id="4813466"/>
    <dbReference type="KEGG" id="dpo:4813466"/>
    <dbReference type="eggNOG" id="KOG3251">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083740_0_1_1"/>
    <dbReference type="InParanoid" id="Q2LZL8"/>
    <dbReference type="OMA" id="LKYDSRH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078428">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZL8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15863">
        <property type="entry name" value="SNARE_GS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.400">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038407">
        <property type="entry name" value="v-SNARE_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001353755.1"/>
        </source>
    </evidence>
    <sequence length="216" mass="24734" checksum="56974E2350395A95" modified="2006-02-21" version="1">MESLYHQTNNTVKEIEHAFQRLGQISTQEALDVENSIQLKITQANSNCDRLDVLLFKVPPSQRQSSKLRVDQLKYDLRHLQTSLQHAKDRRQRREQEISEREQLLNHRFSANSTQPEETCLQLDYDLQHHTQLGSAHRGVDDMISSGSGILESLISQRMTLGGAHKRIQAIGSTLGLSNHTMKLIERRLVEDRKIFLGGMLVTLLIIGLIIYFLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M096</accession>
    <accession>A0A6I8UCZ5</accession>
    <name>Q2M096_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aldose 1-epimerase</fullName>
            <ecNumber evidence="13">5.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC4812826</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mutarotase that catalyzes the interconversion of beta-D-galactose and alpha-D-galactose during galactose metabolism. Beta-D-galactose is metabolized in the liver into glucose 1-phosphate, the primary metabolic fuel, by the action of four enzymes that constitute the Leloir pathway: GALM, GALK1 (galactokinase), GALT (galactose-1-phosphate uridylyltransferase) and GALE (UDP-galactose-4'-epimerase). Involved in the maintenance of the equilibrium between the beta- and alpha-anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1. Also active on D-glucose although shows a preference for galactose over glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>alpha-D-galactose = beta-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:28675"/>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="2">
            <dbReference type="Rhea" id="RHEA:28677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>alpha-D-glucose = beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:10264"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the aldose epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.3" evidence="13"/>
    <dbReference type="RefSeq" id="XP_001353527.2">
        <property type="nucleotide sequence ID" value="XM_001353491.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M096"/>
    <dbReference type="SMR" id="Q2M096"/>
    <dbReference type="FunCoup" id="Q2M096">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M096"/>
    <dbReference type="GeneID" id="4812826"/>
    <dbReference type="KEGG" id="dpo:4812826"/>
    <dbReference type="eggNOG" id="KOG1604">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031753_2_1_1"/>
    <dbReference type="InParanoid" id="Q2M096"/>
    <dbReference type="OMA" id="NWATYQF"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076923">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M096">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000003">
        <property type="entry name" value="Aldose 1-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015443">
        <property type="entry name" value="Aldose_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008277">
        <property type="entry name" value="PRK11055.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF0">
        <property type="entry name" value="GALACTOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005096">
        <property type="entry name" value="GALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="88"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="186"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR005096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001353527.2"/>
        </source>
    </evidence>
    <sequence length="376" mass="41906" checksum="8BCE49F245FFCAA9" modified="2008-10-14" version="2">MPITLEEEIFGLAVNPFTKAPEMVRRYTLTNSNRMSISVIQLGAIIQSVRMPDAYEKVDDICLGFDDIASYVATKGAYIGGTLGRVANRVANGEYVVGETKVELTKNFQNKYQLHGGLVGFDSVIWEVVQKTEDGIVFRHVSAHGHEGYPGTLTCLITYWLDNQNRIGVQFEATTDRKTVVNISNHAYFNLAGHSAGPKGLAEHTVEIAASKIVETDDQQIPTGQLVDVDDTVFDLRLPVLLGDRLMQFGGSLIDGYDNCFVVNGGSTQPGLSMIAKLVHPPSCRVMEVWTNQPGLQFYTANNLPNEKSGEFPMIGKDCTHYVKHGSFCVETEKFPDSMNHPEFPSIFLEPGEKYQHEVMYWFKVEESWKCCCNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0I1</accession>
    <accession>A0A6I8UCS2</accession>
    <name>Q2M0I1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenosine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4813216</name>
        <name evidence="15 16" type="synonym">Adgf-A</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15 16">MV-25-SWS-2005</strain>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenosine + H2O + H(+) = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. ADGF subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001353442.3">
        <property type="nucleotide sequence ID" value="XM_001353406.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042854.1">
        <property type="nucleotide sequence ID" value="XM_015187368.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0I1"/>
    <dbReference type="SMR" id="Q2M0I1"/>
    <dbReference type="STRING" id="46245.Q2M0I1"/>
    <dbReference type="GeneID" id="4813216"/>
    <dbReference type="KEGG" id="dpo:4813216"/>
    <dbReference type="eggNOG" id="KOG1097">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022829_3_0_1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079279">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0I1">
        <property type="expression patterns" value="baseline"/>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01321">
        <property type="entry name" value="ADGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000017">
        <property type="entry name" value="Adenosine deaminase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013659">
        <property type="entry name" value="A_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006331">
        <property type="entry name" value="ADGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01431">
        <property type="entry name" value="adm_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF39">
        <property type="entry name" value="ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08451">
        <property type="entry name" value="A_deaminase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014308676" description="Adenosine deaminase" evidence="11">
        <location>
            <begin position="31"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Adenosine/AMP deaminase N-terminal" evidence="13">
        <location>
            <begin position="52"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Adenosine deaminase" evidence="12">
        <location>
            <begin position="223"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001353442.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015042854.1"/>
        </source>
    </evidence>
    <sequence length="553" mass="63519" checksum="8D201A431B07FDE2" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0R1</accession>
    <accession>A0A6I8UCP4</accession>
    <name>Q2M0R1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="4 17">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Pbgs</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homooctamer; active form. Homohexamer; low activity form.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="4 17"/>
    <dbReference type="RefSeq" id="XP_001353362.2">
        <property type="nucleotide sequence ID" value="XM_001353326.4"/>
    </dbReference>
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        <property type="interactions" value="1029"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0R1"/>
    <dbReference type="GeneID" id="4813288"/>
    <dbReference type="KEGG" id="dpo:4813288"/>
    <dbReference type="eggNOG" id="KOG2794">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_1_1"/>
    <dbReference type="InParanoid" id="Q2M0R1"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070308">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04824">
        <property type="entry name" value="eu_ALAD_PBGS_cysteine_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000048">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001353362.2"/>
        </source>
    </evidence>
    <sequence length="327" mass="36135" checksum="DB5A55C5BB0C712A" modified="2008-10-14" version="2">MDRKLHSGMHHPTLRLLQESGCEISPHNLMYPVFIVSNDDDVQPIASMPGISRFGVNRLRDHLEPLVKKGLTSVLLFGVVESELKDEEASNADSAVNPVVRALPKLREWFPDLLIACDVCICPYSSHGHCGLLGKAGLENGPSIKRIAEIAIAYGQAGAHIVAPSDMMDNRVRAIKQALIDASLNSVSLLAYSAKFASNFYGPFREAAQSAPKFGDRRCYQLPSGSRSLAMRAIQRDVSEGADMLMVKPGMPYLDILRQTKDSYPYHTLYVYQVSGEYAMLYHASKAGAFELRDAVLESMRSFRRAGADCIITYYTPYLLDIIGRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P029</accession>
    <accession>A0A6I8VA11</accession>
    <name>A0A0R3P029_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26533680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036917.1">
        <property type="nucleotide sequence ID" value="XM_015181431.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P029"/>
    <dbReference type="GeneID" id="26533680"/>
    <dbReference type="KEGG" id="dpo:26533680"/>
    <dbReference type="InParanoid" id="A0A0R3P029"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272667">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015036917.1"/>
        </source>
    </evidence>
    <sequence length="50" mass="5491" checksum="BCA2236E99E8AB97" modified="2016-01-20" version="1">MLELVVLFLLSSVSFFVAHLEKPNSFSGYKDATCPIVMGILAAGLVYKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P460</accession>
    <accession>A0A6I8VKR1</accession>
    <name>A0A0R3P460_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Papss</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; sulfate assimilation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015042976.1">
        <property type="nucleotide sequence ID" value="XM_015187490.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P460"/>
    <dbReference type="SMR" id="A0A0R3P460"/>
    <dbReference type="FunCoup" id="A0A0R3P460">
        <property type="interactions" value="843"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P460"/>
    <dbReference type="GeneID" id="4813425"/>
    <dbReference type="InParanoid" id="A0A0R3P460"/>
    <dbReference type="UniPathway" id="UPA00097"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081009">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P460">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050428">
        <property type="term" value="P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000006">
        <property type="entry name" value="bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000004">
        <property type="entry name" value="PAPS synthetase, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00065">
        <property type="entry name" value="Adenylyl_sulf_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059117">
        <property type="entry name" value="APS_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00455">
        <property type="entry name" value="apsK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003013">
        <property type="entry name" value="PRK03846.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055">
        <property type="entry name" value="BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11055:SF1">
        <property type="entry name" value="PAPS SYNTHETASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01583">
        <property type="entry name" value="APS_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APS kinase" evidence="9">
        <location>
            <begin position="69"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="11">
        <location>
            <begin position="256"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="10">
        <location>
            <begin position="419"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015042976.1"/>
        </source>
    </evidence>
    <sequence length="653" mass="73452" checksum="4F4DFD0D03C80832" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P529</accession>
    <accession>A0A6I8VKJ1</accession>
    <name>A0A0R3P529_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aldo-keto reductase family 1 member B1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Akr1B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043305.1">
        <property type="nucleotide sequence ID" value="XM_015187819.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P529"/>
    <dbReference type="SMR" id="A0A0R3P529"/>
    <dbReference type="FunCoup" id="A0A0R3P529">
        <property type="interactions" value="1093"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P529"/>
    <dbReference type="GeneID" id="4812913"/>
    <dbReference type="InParanoid" id="A0A0R3P529"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079339">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P529">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000006">
        <property type="entry name" value="Aldo-keto reductase family 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="7">
        <location>
            <begin position="61"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="6">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015043305.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="41065" checksum="2541C92012D8EA6F" modified="2016-01-20" version="1">MIRVFKPMFGLRLAGAIGQHNPKTMMNIRNYGKPERFMWCQTKVSKVPKAILADGKEMPLIGLGTWLSPKGQVTEAVKVAIDAGYRHIDCAHVYQNEDEVGAGIEAKIKEGVVKREELFITSKLWNTFHRPDLVRNACENTLEALKLKYIDLYLIHWPMGYKEGCDLFPADKEGKTLFSPVDYVDTWKAMEKLVEDGLVKSIGVSNFNKKQIQRVLDVAKIPPATNQIECHPYLTQKKLIDFCKSRDITITAYSPLGSPNRPWAKSGDPVILEEPKIKEIAEKKKKTPGQILIRYQVQRANIVIPKSVTKERIESNFQVFDFVLTPEEIEIIESFDCNGRLVPLLNVYGHPHHPFENDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBD2</accession>
    <name>A0A6I8UBD2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 16">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="16">ATase</shortName>
            <ecNumber evidence="3 16">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 16">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">Prat2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:14907"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="18">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="19">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 16">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 16"/>
    <dbReference type="RefSeq" id="XP_001352839.3">
        <property type="nucleotide sequence ID" value="XM_001352803.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBD2"/>
    <dbReference type="FunCoup" id="A0A6I8UBD2">
        <property type="interactions" value="380"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812104"/>
    <dbReference type="InParanoid" id="A0A6I8UBD2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000047">
        <property type="entry name" value="Amidophosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 19" id="KW-0408">Iron</keyword>
    <keyword evidence="12 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="20">
        <location>
            <begin position="56"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001352839.3"/>
        </source>
    </evidence>
    <sequence length="545" mass="59505" checksum="378D9E62C9BCF783" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBN2</accession>
    <name>A0A6I8UBN2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Histone-lysine N-methyltransferase ash1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ash1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">MV-25-SWS-2005</strain>
            <tissue evidence="24">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352956.4">
        <property type="nucleotide sequence ID" value="XM_001352920.4"/>
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        <property type="component" value="Chromosome X"/>
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    <dbReference type="GO" id="GO:0005694">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018991">
        <property type="term" value="P:egg-laying behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04717">
        <property type="entry name" value="BAH_polybromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15548">
        <property type="entry name" value="PHD_ASH1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19174">
        <property type="entry name" value="SET_ASH1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000011">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000113">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000039">
        <property type="entry name" value="Histone-lysine N-methyltransferase ash1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
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    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
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        <property type="entry name" value="PHD_ASH1L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
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    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
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    </evidence>
    <sequence length="502" mass="56545" checksum="14C971968A019794" modified="2020-08-12" version="1">MDQRSPSPGRNVTASAASQLLSESQVEMRRSQRLIDKESRCKTKTKSLENAGSPQLFDETDDEWSQPLCQLLPLTQIVHQEEPVAIKRRSSSVSVKHVVPLKRRKGRPPAKRTTKNAKNSISFYQELHKSQSEVLKSATQWELGEYREQLQRPANSAQDSLQELQQQRPSSQVVHLKKKCGSLHSSNNNENNPLKQLKGQSASQGKAGQGTSFFKQDQRPVTSECFGETYEEQTKEQVIPLKRRKSRAQSKQTQPEVMQEHCPSTTSPIKDSCLSSSQLQLLAKSYKEYSQRICEQQEDRKTSTAYVEKKVPPLDIPLKPPNEGSSTQGKPKQSSTFKRYGTEFQKQSPSKLPPKYPAHFVHQLPPLGGGDRSYMHMRPRAAPAAQLREMCPTPELTPVPEPQKAPLTTFDDLSPPNPGSESSGTLSLSDSIGEIFGTKNISRILNVECPRQYILIEDHLPAMAMMLNVELVRLRAVLDLTQRLTHEQILNWPLKLEKPTND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE08</accession>
    <name>A0A6I8UE08_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353920.4">
        <property type="nucleotide sequence ID" value="XM_001353884.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE08"/>
    <dbReference type="KEGG" id="dpo:4813866"/>
    <dbReference type="InParanoid" id="A0A6I8UE08"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UE08">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353920.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30441" checksum="70D9471C430D1ED3" modified="2020-08-12" version="1">MSKTQNRKKSKQPRRGPVSSTGSETERSLEDMLSVTRLRIGDTDADADDGERPTRKMKPKSKAPQKSLGARSVEAPDQPPFVALVANLPMETSVRELLVLFVQHSIRSISLLRLGKGGSQQRKAHVELATRDDLMELLKKDQLLCRGRRLSISVCQDPGAAGDERCNRHVVSGLDGTWSLHSGSDADESSSEYAASVRSVQEREASSEASVHISRTPSSTEELERRMEVRLQKLAEFENAQTEQAQSSQGDDDEIPPQMSWPKFWSDVQKSEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE38</accession>
    <name>A0A6I8UE38_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein suppressor of underreplication</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SuUR</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354020.2">
        <property type="nucleotide sequence ID" value="XM_001353984.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE38"/>
    <dbReference type="FunCoup" id="A0A6I8UE38">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812944"/>
    <dbReference type="InParanoid" id="A0A6I8UE38"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UE38">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SNF2 N-terminal" evidence="3">
        <location>
            <begin position="31"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="356"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="681"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="864"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="944"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="585"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="716"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="864"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="944"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="977"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354020.2"/>
        </source>
    </evidence>
    <sequence length="986" mass="110324" checksum="8E663A2F50B43AF3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE86</accession>
    <name>A0A6I8UE86_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cyclin-dependent kinase 12</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cell division protein kinase 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20 21" type="primary">Cdk12</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20 21">MV-25-SWS-2005</strain>
            <tissue evidence="20 21">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001354119.3">
        <property type="nucleotide sequence ID" value="XM_001354083.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043493.2">
        <property type="nucleotide sequence ID" value="XM_015188007.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE86"/>
    <dbReference type="KEGG" id="dpo:4813960"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07864">
        <property type="entry name" value="STKc_CDK12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000102">
        <property type="entry name" value="cyclin-dependent kinase 12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000074">
        <property type="entry name" value="cyclin-dependent kinase 12 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
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            <position position="851"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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</entry>
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            <begin position="34"/>
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    </feature>
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            <begin position="73"/>
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            <begin position="112"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Pfam" id="PF16066"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_001354920.4"/>
        </source>
    </evidence>
    <sequence length="276" mass="29659" checksum="E3DDE0DC28CC0E31" modified="2020-08-12" version="1">MASSSSKPRSHSAAGTQLRWLDCLIQLCLFTRAVRLAACAEEVTTSGSLSEEWGSGWRGTNFLVEVEQTAASLPSGTVSGGGASGSGSGSGTSSGNVEDYLGSQEEDGGFTTGTTPGASNGTTVETGPKIIVRTEEVLIVGLVLVLWVGAIMLFFNRWGKIRMLEPYQPKFQQQHRSSCPLVDLDAVQTHQRTSVSRMSMGMVHNLNMPTCQFAAYNPNMYAKGYVSHVSNASRPRQNSVFVGASTSHYLMPRAPRKTRSAMDLHSMVLDESAEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIE9</accession>
    <name>A0A6I8UIE9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein UXT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Uxt</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356028.3">
        <property type="nucleotide sequence ID" value="XM_001355992.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIE9"/>
    <dbReference type="FunCoup" id="A0A6I8UIE9">
        <property type="interactions" value="388"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816603"/>
    <dbReference type="InParanoid" id="A0A6I8UIE9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073649">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIE9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd23158">
        <property type="entry name" value="Prefoldin_UXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000034">
        <property type="entry name" value="GM15993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356028.3"/>
        </source>
    </evidence>
    <sequence length="186" mass="21747" checksum="3E8EDE350C113FF2" modified="2020-08-12" version="1">MLDASMFFYITISNKNKQTSAKMYENSTGVAYERIQRGPQPDTHQARITQIEEFINEVLKRDLRELENWIGQYNQEIMEYVQLKNTLQTFDAHLPEGYKTQVNIGSNVFMQARVSQMDKILVNVGKDVFLEMSMAEAERFSDVRIKILTKEADVLRDESIKKRTQIKMSLLAIGEREKLMQEEARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIN4</accession>
    <name>A0A6I8UIN4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">FUN14 domain-containing protein fndc-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4816303</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356078.4">
        <property type="nucleotide sequence ID" value="XM_001356042.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIN4"/>
    <dbReference type="KEGG" id="dpo:4816303"/>
    <dbReference type="InParanoid" id="A0A6I8UIN4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356078.4"/>
        </source>
    </evidence>
    <sequence length="130" mass="13759" checksum="830D59783D12E034" modified="2020-08-12" version="1">MSHNFRSKGAFDGFGKQSPYCQIGIGAGTGLVTGYVFFKASKSVAIAAGASILIVQLAMESGLVNFEVETIVMHGHQLNPNNIHVMPSIASSHSWEAIKEQLTKARDFLAGSQRLCVAMLGGFLVGFGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIV7</accession>
    <name>A0A6I8UIV7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cuticle protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Cpr23B</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356162.2">
        <property type="nucleotide sequence ID" value="XM_001356126.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIV7"/>
    <dbReference type="FunCoup" id="A0A6I8UIV7">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816532"/>
    <dbReference type="InParanoid" id="A0A6I8UIV7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF86">
        <property type="entry name" value="CCP84AC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026351607" evidence="4">
        <location>
            <begin position="18"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="33"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="215"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001356162.2"/>
        </source>
    </evidence>
    <sequence length="260" mass="27187" checksum="2A866164C992E8AE" modified="2020-08-12" version="1">MAAKLFVLLSLAVVAQAEYNYNEKAATAVNTLEQSSPSSGEDFLSDYHTPSNNALNSQATPDGYDYVPPRTNQFAAGGSSSASVHASNLLQSAANAASASAALLPSPLPILRQHQEPEVFPPASYSFNYAVNDESTGDIKEHSETRDGYVVRGFYSLIDPDGYKRTVTYTADDVHGFNAVVNRVPYALKTVAVAVAPSVVPAIALDERSKLQTVDITRSSGASPVESEGSEGSGSGSGAFSHDSYANAPRGLDASGGPYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJ15</accession>
    <name>A0A6I8UJ15_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Meiosis regulator and mRNA stability factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Marf1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356295.4">
        <property type="nucleotide sequence ID" value="XM_001356259.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UJ15">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816434"/>
    <dbReference type="InParanoid" id="A0A6I8UJ15"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08824">
        <property type="entry name" value="LOTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045602">
        <property type="entry name" value="MARF1_LOTUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19687">
        <property type="entry name" value="MARF1_LOTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH OST-type" evidence="2">
        <location>
            <begin position="527"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="2">
        <location>
            <begin position="603"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="2">
        <location>
            <begin position="690"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="2">
        <location>
            <begin position="771"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1031"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356295.4"/>
        </source>
    </evidence>
    <sequence length="1292" mass="143557" checksum="1E6E1692CE7DB707" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJD1</accession>
    <name>A0A6I8UJD1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc carboxypeptidase A 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC4817147</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356460.3">
        <property type="nucleotide sequence ID" value="XM_001356424.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJD1"/>
    <dbReference type="FunCoup" id="A0A6I8UJD1">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817147"/>
    <dbReference type="InParanoid" id="A0A6I8UJD1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJD1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.340:FF:000002">
        <property type="entry name" value="Carboxypeptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF123">
        <property type="entry name" value="PEPTIDASE M14 CARBOXYPEPTIDASE A DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 18" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026165555" description="Zinc carboxypeptidase A 1" evidence="15">
        <location>
            <begin position="21"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="16">
        <location>
            <begin position="125"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001356460.3"/>
        </source>
    </evidence>
    <sequence length="426" mass="48290" checksum="BA6D8A93DD5E342C" modified="2020-08-12" version="1">MVNMRLLWLCAATLVVLASAGTLKDTPKQRFDNYGVYKLTIRNRIQLALIEKIGEISNKYDIWKEYDEHTKEVHIMVSPEELKHFQELLKVNDISSEQMIENVQELIDGEQVTAAADSGTFGWTKYYELSEIEAWLDSVLAAYPTVTEEFIVGQSYEGRTIRGIKISHKAGNPGIFIESNIHAREWITSASATWFINQLLTSEEPAVRNLAESYDWHIVPVFNVDGFEYSHAKNRMWRKTRQPHATNECIGADANRNFDSHWLENNGASSNPCSETFAGDEPGSEPEAKALVEYLTQIKDQFSVYISFHSYGQYLLSPYGHTAAEFPDNYADLLEIGEAFATAIEDLPYGTVYRYGSTADVLYVATGTSVDWVFNELDKKVAFTIEYRDQGRYGFILPPVQIIPNCEELMAGMLALIDKTKELGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULT0</accession>
    <name>A0A6I8ULT0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4800330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357581.4">
        <property type="nucleotide sequence ID" value="XM_001357544.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULT0"/>
    <dbReference type="SMR" id="A0A6I8ULT0"/>
    <dbReference type="FunCoup" id="A0A6I8ULT0">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800330"/>
    <dbReference type="InParanoid" id="A0A6I8ULT0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHCH" evidence="3">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357581.4"/>
        </source>
    </evidence>
    <sequence length="188" mass="19141" checksum="3B71435740E15A6A" modified="2020-08-12" version="1">MPRQRSDKSGGSARRQSSRSNSSCTIPVPSSRGSSSRLPATQPRKQESTPVATPAATPATTPATTPAAAAASAPEAATKGRGPGSMFKDMATTAAGVAAGSAVGHAVGAGITGLFGGGRGSSVPASQQGEQAAPPVPVQAQRSGEFVEDGPCAYELRQFLKCTEENSDLSVCQGFNEAMKDCRRRYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UME7</accession>
    <name>A0A6I8UME7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome c1, heme protein, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Complex III subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Complex III subunit IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cytochrome b-c1 complex subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">Cyt-c1L</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">MV-25-SWS-2005</strain>
            <tissue evidence="27">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="21">Binds 1 heme c group covalently per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with FLVCR2; this interaction occurs in the absence of heme and is disrupted upon heme binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357732.2">
        <property type="nucleotide sequence ID" value="XM_001357695.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UME7"/>
    <dbReference type="FunCoup" id="A0A6I8UME7">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800460"/>
    <dbReference type="InParanoid" id="A0A6I8UME7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000002">
        <property type="entry name" value="Cytochrome c1, heme protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000003">
        <property type="entry name" value="Cytochrome c1, heme protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021157">
        <property type="entry name" value="Cyt_c1_TM_anchor_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266">
        <property type="entry name" value="CYTOCHROME C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266:SF3">
        <property type="entry name" value="CYTOCHROME C1, HEME PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81496">
        <property type="entry name" value="Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 21 22" id="KW-0349">Heme</keyword>
    <keyword evidence="12 21 22" id="KW-0408">Iron</keyword>
    <keyword evidence="14 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 21 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="25">
        <location>
            <begin position="90"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="311"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="326"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
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    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
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    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="21">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040084"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062753"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00079825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR602326-1"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_001357732.2"/>
        </source>
    </evidence>
    <sequence length="354" mass="39106" checksum="DEC46DBB3E42735F" modified="2020-08-12" version="1">MANKFGHAISKLLQLSKGYLKRSPVRPKSSFSAPNWIPGRSKWLAFGALTGGLGALLYALDASVDASHDCVHPPKQFWNHKGLLSALDKEAVRRGYQVYKEVCSSCHSLQYIAYRNLVNNCMTEAEAKAQAEQVMVKDGPDENGEYFERPGKLSDYLPSPYPNEDAARSANNGAYPPDLSYIVSARKGGEDYIFALLTGYCDPPAGFVLRDGQYFNPYFSGGAISMGQVINNEVVTYADEEVAPSGSQIAKDVVTFLKWTSEPEADERKLLLIRVFLICSFLIGLSYYIKRHKWSALKSRKIFYVPEVTGNGNGQSKGNGQGNAKAKTDEACPKHEHNKKEGNGKNNDGDWKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNI4</accession>
    <name>A0A6I8UNI4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="3">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Gfat2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001358242.1">
        <property type="nucleotide sequence ID" value="XM_001358205.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNI4"/>
    <dbReference type="FunCoup" id="A0A6I8UNI4">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="GeneID" id="4801083"/>
    <dbReference type="KEGG" id="dpo:4801083"/>
    <dbReference type="InParanoid" id="A0A6I8UNI4"/>
    <dbReference type="OMA" id="HLWQTHP"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072343">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000126">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="356"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="527"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358242.1"/>
        </source>
    </evidence>
    <sequence length="678" mass="75742" checksum="FEB7B0C129D64124" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNI7</accession>
    <name>A0A6I8UNI7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pll</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001358369.3">
        <property type="nucleotide sequence ID" value="XM_001358332.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNI7"/>
    <dbReference type="FunCoup" id="A0A6I8UNI7">
        <property type="interactions" value="1839"/>
    </dbReference>
    <dbReference type="GeneID" id="4801246"/>
    <dbReference type="KEGG" id="dpo:4801246"/>
    <dbReference type="InParanoid" id="A0A6I8UNI7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079269">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000754">
        <property type="entry name" value="Interleukin-1 receptor-associated kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000572">
        <property type="entry name" value="Interleukin-1 receptor-associated kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.530">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Death" evidence="14">
        <location>
            <begin position="50"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="208"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="131"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001358369.3"/>
        </source>
    </evidence>
    <sequence length="500" mass="56774" checksum="53BE4DC2417B9E03" modified="2020-08-12" version="1">MMSGSETAEAQGTANRTRSRSHQDDTLLIRLLPVVARHQLCVHLDSLDVWQQLATAVKLYPEQVEQIRHQKDRGRSPANEFMNIWGGQYNHTVQSLFALFKKLKLHHAMRMIKDYVNEDLHKYIPRSVPTISDLRAPPDSNSKIDNGPPFPSSSGVSNSNNNRTTTTMSEEIPSLESLGNIHISTVQRAAESLLKIDYSELERATDSWCPENRLGQGGFGEVYKGEWKQLDVAIKVMNYRSPNIDKNQVELQQSYNELKYLNTIRHDNILALYGYSINGDKPCLVYQLMKGGSLENRLRAHKSQQPLPALTWMQRFSICHGTAKGIYFLHTARSTPLIHGDIKPANILLDQCLQPKIGDFGLAREGPKSINAVMQVKKVFGTRIYLPPEFRNSKQLSTGVDVYSFGIVLLEVFTGRQVTDRLPKNDQHQDLLHYVKQNWAQSQQNRLELLDKHIPKPVGDELDMCLCAIEAGLQCTALETQDRPSMNGVLNRFKPFRIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNQ8</accession>
    <name>A0A6I8UNQ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein beat-IIb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">beat-IIb</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358338.4">
        <property type="nucleotide sequence ID" value="XM_001358301.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNQ8"/>
    <dbReference type="FunCoup" id="A0A6I8UNQ8">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801212"/>
    <dbReference type="InParanoid" id="A0A6I8UNQ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002398">
        <property type="entry name" value="Beat IIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261">
        <property type="entry name" value="BEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261:SF6">
        <property type="entry name" value="BEATEN PATH IIA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358338.4"/>
        </source>
    </evidence>
    <sequence length="473" mass="50991" checksum="586041509A3B5F57" modified="2020-08-12" version="1">MSNLKSSRAAFKRTTATTTARKVAKTTVTMWQTLSTLSTSLTAAILGPGWPPSPSQSPSPPGCRCGVHRLKGKLRGAKASTYHAEMQSHRVTSNMLPSTDIATAATVARRCRCGSHITVTINNNNNTNNNSHTIKKNSNISGGSLLHWLLLLCVFCDAGQATLRDVNLLVQPPAVRRGQSVALRCDYQLDGAPLYSIKFYRGQMEFYRYTPGEYPHTKVFQYPGIRVDEGSSNATLVLIRNVSFGLSGQFSCEVTADAPLYSTATAYAQMQVVEFPEKRPQLFTEHTRYEPGDVLRANCSTPPSRPRSELRFTINNVPIPTEETQYIRTIDNLIASRLSLKLQLQAVHFVAGINGHGNGNVNGNGNGNGNGNGNSNGIANALVTGSGSGSGGGLVLRCTAQIGDLYQEYKEIELGTPQKDPVPARVTLSSGTGLRSFLEAYFTTSTAAVAWQGGITIWLLSTLALVINCSRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNW2</accession>
    <name>A0A6I8UNW2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4801340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358451.2">
        <property type="nucleotide sequence ID" value="XM_001358414.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNW2"/>
    <dbReference type="KEGG" id="dpo:4801340"/>
    <dbReference type="InParanoid" id="A0A6I8UNW2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20987">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20987:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026210353" description="Chitin-binding type-2 domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="40"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358451.2"/>
        </source>
    </evidence>
    <sequence length="231" mass="24774" checksum="C71FEB7E201EA4F9" modified="2020-08-12" version="1">MKVFALALLCLAAVAQAHISYKLQCARGEIGIRWPSYHSNSEYYVCQGIYGSQLTIHCPAGEVFSFVLQQCASPSQYVPAPPINILPTAAPITMTVVEDAPPVIGAGIATSAHNQIEQHETAEHHEIAGPHEHHEQNELHELHEHHEHEHQTEQEVVAHPIPPTPAPEPPVVESAVKPASAPIPNKGVKKPVVPVPAKKATSAKKPVAPIPSKTAKKPTPPSKAQKKPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNW7</accession>
    <name>A0A6I8UNW7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pollen-specific leucine-rich repeat extensin-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801461</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358553.3">
        <property type="nucleotide sequence ID" value="XM_001358516.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNW7"/>
    <dbReference type="KEGG" id="dpo:4801461"/>
    <dbReference type="InParanoid" id="A0A6I8UNW7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="418"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358553.3"/>
        </source>
    </evidence>
    <sequence length="486" mass="53658" checksum="D28DAF06D943FDA6" modified="2020-08-12" version="1">MSLANHSQNLQLQLEQLRELQQLQEQQQQLEGQHPLLSAIAENNYAQTQRVHYPNHYYPSNHYHYQQQGRPLLAAAGSSHNGAYAGTAPYSGHSGHGHIEPSVEYELQQTPPSGGFTSYHASAGSAPALSMGMPHPPPHPPRPPPHPPAIHSYSYPKPPPPTRGSAGKKSKKNSSGVMSALTLLSFFFFLNLLQNCIKDHMSDMNPTVMVLTDTGSRNRFNKLAEMNSREQTSSPTPSWQQAALVPVPDSGPGSVPVSSPAPVIVKPTVVLQSPYSGAAASAAAVVTNKPQYHQNQHHPHPHPHPHPHPHPHPHQHHNHNPATDSHHYPPPPGDDDDDVDYNYGHRRPPPGPPQTSAADFYPNRTTHIDHSSRPDFDSDSDSDYYQHHYYPERHRYSDSRYSHPRRPWSYVGAASHAGSGSRQRYSSAPWSSASLGAQSGVSSYLDNAQRRQGDDDDAAAGHGFDADDDDGRHHRRRGDAFYTRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR36</accession>
    <name>A0A6I8UR36_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Mst89B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Mst89B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359466.3">
        <property type="nucleotide sequence ID" value="XM_001359429.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR36"/>
    <dbReference type="FunCoup" id="A0A6I8UR36">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802571"/>
    <dbReference type="InParanoid" id="A0A6I8UR36"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359466.3"/>
        </source>
    </evidence>
    <sequence length="329" mass="35774" checksum="6576B39C54EB5AE2" modified="2020-08-12" version="1">MANNVKKLLQVSEEAAPLIQIPVMVGNEMFVLTQQDVRRTEDIRYLSYDRIAPNVHLFPTAAPAAAPVNYFGGLFTPLALPCTAPPNGPLQELFGRKKAEPAPGNVADVATQMAWSKEKEIEPSNRAPLRYSAATSTSSLTKSCACQHDPPKSRSQHTSCNSHTARNACVQTKGTTSSTASSTGSKSIWKTVASDMSVAESKSNVNISSPANLSDAGSKDNCNGSSEGSVPAKESKCPSRHCKSQQTCTYSDGQSYHRCSPREGRFRPQQHQSSRCAQYKSRGCAQDRIRAQSQEQLTAAQQDQRSYQRDKAKFSRSVYFSPLEDASSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URL4</accession>
    <name>A0A6I8URL4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phenoloxidase-activating factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prophenoloxidase-activating factor II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4803015</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359817.4">
        <property type="nucleotide sequence ID" value="XM_001359780.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URL4"/>
    <dbReference type="KEGG" id="dpo:4803015"/>
    <dbReference type="InParanoid" id="A0A6I8URL4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000038">
        <property type="entry name" value="Serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041515">
        <property type="entry name" value="PPAF-2-like_Clip"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18322">
        <property type="entry name" value="CLIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026158662" description="Phenoloxidase-activating factor 2" evidence="8">
        <location>
            <begin position="25"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="158"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359817.4"/>
        </source>
    </evidence>
    <sequence length="401" mass="41540" checksum="FC80ADA94D6F3E67" modified="2020-08-12" version="1">MTKFSCLLLLTLLLQLLHIDGGHGWSFGPVQEAEDEPGGAEPQPSDVASTANHTILTRQLIVGTLVPPLLPGPWPSPAIVAPGTSYCQCVPPGSCANPLPTVPSDGSGQLDIRIVNTGAYPSIPSTSATLTCSYGQVACCQAGSYQCGRRFPPPLGSSTAGAGQANFGAYPWQAALLTTADVYLGGGALITAQHVLTAAHKVYNLALTSFKVRLGEWDASSTSEPIPAQDVYVSNVYVNPSFNPTNLQNDVAILKLVQAVSLTSKSTVGTICLPTTSFVGQRCWVAGWGKNDFGPTGAYQAIQRQVDVPLIPNANCQAALRATRLGSSFALSPTSFICAGGEAGKDACTGDGGSPLVCTSNGLWYVVGLVAWGIGCAQAGVPGVYVNVGTFLPWIQTTLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US00</accession>
    <name>A0A6I8US00_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4803228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360004.2">
        <property type="nucleotide sequence ID" value="XM_001359967.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US00"/>
    <dbReference type="GeneID" id="4803228"/>
    <dbReference type="KEGG" id="dpo:4803228"/>
    <dbReference type="InParanoid" id="A0A6I8US00"/>
    <dbReference type="OMA" id="LNGNRYM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077946">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360004.2"/>
        </source>
    </evidence>
    <sequence length="254" mass="28436" checksum="1C6FB11F5EF38043" modified="2020-08-12" version="1">MGDSPPGRQTIGRPEFYKKVYTILVIFIVTGLLQLVIVKACDYDFYSGFPVSSTVWVLIALWCLLTLLCVPVTLAFPITYVLGIIAAEALTLSVLSQDFLLLTMAWTYSILAVAVILSICLYVIGIFMPLKFLPGSPTMLGLGIAAIIVLASLLITRFATGNEAMMFYFNIVSLLYFMLIVVFTITVVHDRRLQPLPQEKRMVPVIILAALFFYMIRMIAVLVLYAVRDLSIKSAINPGKLQKSFFQKRYIPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTI1</accession>
    <name>A0A6I8UTI1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Lysosomal aspartic protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4803883</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360531.2">
        <property type="nucleotide sequence ID" value="XM_001360494.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTI1"/>
    <dbReference type="FunCoup" id="A0A6I8UTI1">
        <property type="interactions" value="686"/>
    </dbReference>
    <dbReference type="GeneID" id="4803883"/>
    <dbReference type="KEGG" id="dpo:4803883"/>
    <dbReference type="InParanoid" id="A0A6I8UTI1"/>
    <dbReference type="OMA" id="RAYWQVK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070133">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000149">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000002">
        <property type="entry name" value="Vacuolar aspartic proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9 13" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026332191" evidence="10">
        <location>
            <begin position="17"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="11">
        <location>
            <begin position="79"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="110"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="274"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="317"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001360531.2"/>
        </source>
    </evidence>
    <sequence length="399" mass="44947" checksum="08526A0076B612F6" modified="2020-08-12" version="1">MWLLFLSLILPPLVAPSPSTELYRVPLRRFPSARNRFVQFGIRMDRFRLKYSRVDGRSRPRGGWEVRSEPLSNYLDAQYFGPITIGSPPQTFKVIFDTGSSNLWVPSTSCAPTMVACMVHSRYNARQSSSHRRNGVRFAIHYGSGSLAGYLSSDTVRVAGLEIQNQTFAEVTTMPGPIFLAAKFDGIFGLAYQSISMQDVKPPFYAIMEQKLLSNPVFSVYLNRQQEHPEGGALFFGGSNPRYYRGNFTYVPVSHRAYWQVRMEAATINDLRLCQHGCEVIIDTGTSFLALPYDQAILINESIGGTPSEYGQYSVPCDQVPQLPRLTFQLGSQQFFLDGSNYIFRDVYQDREICFSAIIGVDLPSPSGPLWILGDVFLGKYYTEFDMGNHRIGFAEAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTZ0</accession>
    <name>A0A6I8UTZ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FXNA-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC4804263</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360847.3">
        <property type="nucleotide sequence ID" value="XM_001360810.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTZ0"/>
    <dbReference type="FunCoup" id="A0A6I8UTZ0">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804263"/>
    <dbReference type="InParanoid" id="A0A6I8UTZ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000008">
        <property type="entry name" value="Endoplasmic reticulum metallopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053973">
        <property type="entry name" value="ERMP1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053974">
        <property type="entry name" value="ERMP1_1-A_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF22">
        <property type="entry name" value="ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22249">
        <property type="entry name" value="ERMP1-TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22248">
        <property type="entry name" value="ERMP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="36"/>
            <end position="58"/>
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        <location>
            <begin position="525"/>
            <end position="544"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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            <end position="616"/>
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            <begin position="623"/>
            <end position="647"/>
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        <location>
            <begin position="153"/>
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            <begin position="425"/>
            <end position="635"/>
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            <begin position="651"/>
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    <sequence length="881" mass="99541" checksum="D4E3214DE7CD8BD4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU00</accession>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">MV2-25</strain>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="6">MV-25-SWS-2005</strain>
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            <begin position="1"/>
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    <accession>A0A6I8UUD2</accession>
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        </recommendedName>
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        <name evidence="19" type="primary">Inos</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Key enzyme in myo-inositol biosynthesis pathway that catalyzes the conversion of glucose 6-phosphate to 1-myo-inositol 1-phosphate in a NAD-dependent manner. Rate-limiting enzyme in the synthesis of all inositol-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10716"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="5.5.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
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        <property type="reaction ID" value="UER00787"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246155">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR11510">
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    </dbReference>
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        <property type="entry name" value="Inos-1-P_synth"/>
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    </dbReference>
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        <property type="entry name" value="NAD_binding_5"/>
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    </dbReference>
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        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0398">Inositol biosynthesis</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="17">
        <location>
            <begin position="311"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001360777.1"/>
        </source>
    </evidence>
    <sequence length="564" mass="61931" checksum="2D4760E3CCC7C952" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW33</accession>
    <name>A0A6I8UW33_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neurogenic differentiation factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">HLH54F</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361779.5">
        <property type="nucleotide sequence ID" value="XM_001361742.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW33"/>
    <dbReference type="FunCoup" id="A0A6I8UW33">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805368"/>
    <dbReference type="InParanoid" id="A0A6I8UW33"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11423">
        <property type="entry name" value="bHLH_TS_musculin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF72">
        <property type="entry name" value="HLH54F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361779.5"/>
        </source>
    </evidence>
    <sequence length="249" mass="27068" checksum="74DEDD471C151A05" modified="2020-08-12" version="1">MPRKKRSSSPLDFYDDDFDEDASSQSSQPPVQRNAANARERMRMRVLSSAYGRLKTKLPNIPPDTKLSKLDTLRLATLYIKQLITAVETGSHSHSHNHSHSNTAGMDALDTSHMPESSHGCGSGGANVGASLSGGGGNFHFHNGGHSGMSWPFEFHQSNRSLPFGSSRMDWQSLQAQSYPKSARSELDVPAALSYAQMQDSHGHWYPSEMNQMEVTATINGCAYETATAGMGQHQRGIAIATSHAHGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX28</accession>
    <name>A0A6I8UX28_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein peste</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">santa-maria</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132423.2">
        <property type="nucleotide sequence ID" value="XM_002132387.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX28"/>
    <dbReference type="FunCoup" id="A0A6I8UX28">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903551"/>
    <dbReference type="InParanoid" id="A0A6I8UX28"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UX28">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF93">
        <property type="entry name" value="GH07959P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="446"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="520"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="551"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002132423.2"/>
        </source>
    </evidence>
    <sequence length="566" mass="63536" checksum="A906A7F409B91860" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXQ2</accession>
    <name>A0A6I8UXQ2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MYCBP-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132368.2">
        <property type="nucleotide sequence ID" value="XM_002132332.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXQ2"/>
    <dbReference type="KEGG" id="dpo:6903489"/>
    <dbReference type="InParanoid" id="A0A6I8UXQ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXQ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032707">
        <property type="entry name" value="MYCBPAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48421">
        <property type="entry name" value="MYCBP-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48421:SF1">
        <property type="entry name" value="MYCBP-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14646">
        <property type="entry name" value="MYCBPAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132368.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="768" mass="88798" checksum="CAB938823D8BD092" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXS6</accession>
    <name>A0A6I8UXS6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">btv</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132927.3">
        <property type="nucleotide sequence ID" value="XM_002132891.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UXS6">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903226"/>
    <dbReference type="InParanoid" id="A0A6I8UXS6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXS6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000235">
        <property type="term" value="C:astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902850">
        <property type="term" value="P:microtubule cytoskeleton organization involved in mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000996">
        <property type="entry name" value="Cytoplasmic dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000022">
        <property type="entry name" value="DHC7 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002862">
        <property type="entry name" value="Dynein heavy chain family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF22">
        <property type="entry name" value="DYNEIN CYTOPLASMIC 2 HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="1650"/>
            <end position="1787"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="1940"/>
            <end position="2211"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="2247"/>
            <end position="2411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2866"/>
            <end position="2888"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="991"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="3023"/>
            <end position="3099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="2871"/>
            <end position="2883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_002132927.3"/>
        </source>
    </evidence>
    <sequence length="4227" mass="478162" checksum="409CF6A0B965277F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZA1</accession>
    <name>A0A6I8UZA1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Endothelial lipase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6901389</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134077.3">
        <property type="nucleotide sequence ID" value="XM_002134041.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZA1"/>
    <dbReference type="KEGG" id="dpo:6901389"/>
    <dbReference type="InParanoid" id="A0A6I8UZA1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF150">
        <property type="entry name" value="FI01825P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026258408" evidence="5">
        <location>
            <begin position="17"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="29"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002134077.3"/>
        </source>
    </evidence>
    <sequence length="314" mass="34081" checksum="F649D1F5DEDA97D7" modified="2020-08-12" version="1">MMWIALLSLCLCRLSGIEVLATGQVGDMASFYMYTPNSPEHPQRVYTNDSKSVNASHFDAHLPTKVIIHGWSVTYLDAPVSKLRTAYQARGAFNIIAVDWGVIAALSYLEARPMVPGVGKSVARFLSFLANEFSLDLGQVVVVAHSMGAHVAGFCGKELNTTSKGQHPLGYIVALDPALPLFRIPSESLRLSSTDAKYVVAIHTNGLMKGQLMPMGHSDFYANGGRRQPGCGFDLNNSCAHARAVLFYAEAVQHVSRYTPYARCTGYPEFLLLLGNCDGAAPKSVALGDPLDVGRTEGIYSFKTHEESPYGEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZW1</accession>
    <name>A0A6I8UZW1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial import receptor subunit TOM70-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6901720</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134388.2">
        <property type="nucleotide sequence ID" value="XM_002134352.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZW1"/>
    <dbReference type="KEGG" id="dpo:6901720"/>
    <dbReference type="InParanoid" id="A0A6I8UZW1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="325"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="400"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002134388.2"/>
        </source>
    </evidence>
    <sequence length="542" mass="61376" checksum="BED39F4BE6EA3E9B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V032</accession>
    <name>A0A6I8V032_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein 391</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">dwg</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133956.2">
        <property type="nucleotide sequence ID" value="XM_002133920.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V032"/>
    <dbReference type="FunCoup" id="A0A6I8V032">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901255"/>
    <dbReference type="InParanoid" id="A0A6I8V032"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V032">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002456">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1800.20:FF:000019">
        <property type="entry name" value="Zeste-white 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000770">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000193">
        <property type="entry name" value="Zinc finger protein 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002075">
        <property type="entry name" value="zinc finger protein 646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF152">
        <property type="entry name" value="SI:DKEY-210J14.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="15">
        <location>
            <begin position="11"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="531"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002133956.2"/>
        </source>
    </evidence>
    <sequence length="564" mass="63503" checksum="BBE082375DD320B4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0D7</accession>
    <name>A0A6I8V0D7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Trypsin eta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6901467</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134153.3">
        <property type="nucleotide sequence ID" value="XM_002134117.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0D7"/>
    <dbReference type="KEGG" id="dpo:6901467"/>
    <dbReference type="InParanoid" id="A0A6I8V0D7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0D7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA"/>
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    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
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        <property type="entry name" value="Peptidase_S1A"/>
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        <property type="entry name" value="Trypsin_dom"/>
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        <property type="entry name" value="TRYPSIN_SER"/>
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    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRYPSIN_SER"/>
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    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026053155" evidence="8">
        <location>
            <begin position="24"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="38"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002134153.3"/>
        </source>
    </evidence>
    <sequence length="316" mass="33698" checksum="49C9B3A1FC5BA65A" modified="2020-08-12" version="1">MQMHPLWFGALVAVITLLGPTVASTESNSTTQMLQPKIVGGTAIDIKAAPYQVSVRLTSRDKRKFGSGHMCGGVLISQRLVATAAHCCYNSDTKQYRAAGEYVLVLGTTYLGTKTNETLVYYLQQLIVHSSYDHGTLTNDIALMFINGYVPWTWPTARALTLNDQSLAVATACVITGWGVLISGGSSSNTLQTATVPTVSYPNCYLAYPPYVPRSQICAGYTTGGIDACQGDSGGPLMCNNRLSGLVSYGDGCGKPNVPGVYTNVSYFHDWIVQQNSSLNYTIYRNGADGRSGGRLGFVLLSGIFAAAAGSLRIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2B2</accession>
    <name>A0A6I8V2B2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135424.3">
        <property type="nucleotide sequence ID" value="XM_002135388.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2B2"/>
    <dbReference type="KEGG" id="dpo:6900060"/>
    <dbReference type="InParanoid" id="A0A6I8V2B2"/>
    <dbReference type="OMA" id="MEASVAY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007970">
        <property type="entry name" value="DUF733"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05306">
        <property type="entry name" value="DUF733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135424.3"/>
        </source>
    </evidence>
    <sequence length="101" mass="11892" checksum="1E0DB4F618A97F74" modified="2020-08-12" version="1">MADIQQSASSMESSSGTAYALFLHRQELQRRQMLYTKVSRTKIQLTKELIAKQKQRLGACTNEDLEILNRETQFKRTLQQKLKHRSKQLKAIARQQERRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2K6</accession>
    <name>A0A6I8V2K6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="12">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6897088</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_002137272.2">
        <property type="nucleotide sequence ID" value="XM_002137236.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2K6"/>
    <dbReference type="FunCoup" id="A0A6I8V2K6">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897088"/>
    <dbReference type="InParanoid" id="A0A6I8V2K6"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="490"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="566"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="594"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="617"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="643"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="761"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="801"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="834"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="875"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="14">
        <location>
            <begin position="416"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="725"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002137272.2"/>
        </source>
    </evidence>
    <sequence length="928" mass="106005" checksum="9007103836F4D3B1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V345</accession>
    <name>A0A6I8V345_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM98B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM98 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137667.2">
        <property type="nucleotide sequence ID" value="XM_002137631.3"/>
    </dbReference>
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        <property type="interactions" value="1455"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="InterPro" id="IPR018797">
        <property type="entry name" value="FAM98"/>
    </dbReference>
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        <property type="entry name" value="FAM98"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN FAM98B"/>
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    </dbReference>
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        <property type="entry name" value="DUF2465"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="291"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="333"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="370"/>
            <end position="391"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="392"/>
            <end position="406"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="408"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137667.2"/>
        </source>
    </evidence>
    <sequence length="451" mass="49840" checksum="2379888A6537E9B8" modified="2020-08-12" version="1">MELELDVVDSLQALAYDGNCLEQEALSKALDNGIDDEDLRAVIFWLAHELHVLRKTDEHVTSSKKDHDEFAIELSLLLTELSCPYSQLVMGPISDRFDSRESLLQLLDYLTSELMFTKISLKTQPVGPPSKKSKTESNVEATVLELVRDLQLGELPKNISAKAMFDKITPRLEQRMGQTNRGVVSEPLLNLKKLLSDVQWRMLDALHIDLDSEYNLRRQMMLTRLEATIQSFKWSESMRNRENEIMELFQLKLKELEQFKLGGNETNIVALLAARADLAIIEKTSSANVRKNTASKIQKHMIGSVPDRGGRANEHAPPPPEMPSWQQQRASGPQGGGGGRGGGNFRGGGRGGGRGGGRGGGAASGGDNWQPQQPHNQPHQQQHQQQHQQPQQHRDRDNSRDRDRDQQWTSGSGRVQGSGWNPQGGRGGGGRGGGRGRGGGGGGYHQRGGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V451</accession>
    <name>A0A6I8V451_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Nose resistant to fluoxetine protein 6</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">LOC6898357</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138410.2">
        <property type="nucleotide sequence ID" value="XM_002138374.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V451"/>
    <dbReference type="KEGG" id="dpo:6898357"/>
    <dbReference type="InParanoid" id="A0A6I8V451"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="483"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="518"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="587"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="629"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Nose resistant-to-fluoxetine protein N-terminal" evidence="3">
        <location>
            <begin position="67"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="678"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002138410.2"/>
        </source>
    </evidence>
    <sequence length="700" mass="77792" checksum="55A6DE3AD919CA34" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4L5</accession>
    <name>A0A6I8V4L5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6898755</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138737.2">
        <property type="nucleotide sequence ID" value="XM_002138701.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4L5"/>
    <dbReference type="FunCoup" id="A0A6I8V4L5">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898755"/>
    <dbReference type="InParanoid" id="A0A6I8V4L5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V4L5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004210">
        <property type="entry name" value="BESS_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF60">
        <property type="entry name" value="SI:CH211-15D5.12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51031">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="3">
        <location>
            <begin position="22"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="BESS" evidence="4">
        <location>
            <begin position="446"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002138737.2"/>
        </source>
    </evidence>
    <sequence length="578" mass="61248" checksum="824B064E9D0701FE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5L9</accession>
    <name>A0A6I8V5L9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Progestin and adipoQ receptor family member 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6897312</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
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    <sequence length="524" mass="54970" checksum="DDFDB85EC748F70F" modified="2020-08-12" version="1"> </sequence>
</entry>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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        </source>
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        <source>
            <dbReference type="RefSeq" id="XP_003736732.2"/>
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    <sequence length="181" mass="19788" checksum="6B1FE21AA4C329AA" modified="2020-08-12" version="1">MAHRIWTVTIKRHPLLSVPTIKILNVQVNRTLMERKNLQPFFTKPSVVFSKEALAHHWGAVPIVVITLVGFTLEVLSWIRIAVTRDDVWWTKHSGNCMFVETRKGYPAPNRKFLTFNQKYETPPGLIEALQGNTDGPSNEDEKADKGSGGGTSGKKIATHVILTGAALGVAAAGAVYSGAI</sequence>
</entry>
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            <taxon>Endopterygota</taxon>
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                <consortium name="RefSeq"/>
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                <consortium name="RefSeq"/>
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    </dbReference>
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        <location>
            <begin position="103"/>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004334"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51030"/>
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        <source>
            <dbReference type="PROSITE" id="PS51843"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_004444300.2"/>
        </source>
    </evidence>
    <sequence length="579" mass="62843" checksum="AC5C2AF6556031EA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6P5</accession>
    <name>A0A6I8V6P5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">High mobility group protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">HmgD</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_004444433.1">
        <property type="nucleotide sequence ID" value="XM_004444376.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234525.1">
        <property type="nucleotide sequence ID" value="XM_033378634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6P5"/>
    <dbReference type="GeneID" id="15382902"/>
    <dbReference type="KEGG" id="dpo:15382902"/>
    <dbReference type="OMA" id="PLSAYMH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0264590">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21994">
        <property type="entry name" value="HMG-box_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000036">
        <property type="entry name" value="high mobility group protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF20">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="69"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_004444433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033234525.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12385" checksum="C59D88C609C7BB80" modified="2020-08-12" version="1">MADKPKRPLSAYMLWLNSAREGIKRESPGIKVTEVAKRGGELWRAMKDKSEWEAKAAKAKDDYDRAVKDFEANGGNNSANGNAKKRSKPAKKPAKKTKKDDSDDDDDDESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V748</accession>
    <name>A0A6I8V748_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">C-type lectin 37Da-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC26533356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035231.2">
        <property type="nucleotide sequence ID" value="XM_015179745.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V748"/>
    <dbReference type="KEGG" id="dpo:26533356"/>
    <dbReference type="InParanoid" id="A0A6I8V748"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V748">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026281378" evidence="1">
        <location>
            <begin position="21"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="45"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015035231.2"/>
        </source>
    </evidence>
    <sequence length="143" mass="16394" checksum="047939B9268B7A57" modified="2020-08-12" version="1">MYRKLTGVLALLGFLSLSGGYRVISNVMDGVPEFVNINPAPFVKIGNGYYYIENKLRKNWFDAYESCSRMGAALISFETIEEWNLINKYLKDEKIDYFYWTSGTDLAKQGKHVWLSTGNPIALDIWHPSQPDNIEGVEHCDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VA34</accession>
    <name>A0A6I8VA34_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117183181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036968.2">
        <property type="nucleotide sequence ID" value="XM_015181482.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VA34"/>
    <dbReference type="KEGG" id="dpo:117183181"/>
    <dbReference type="InParanoid" id="A0A6I8VA34"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VA34">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16021">
        <property type="entry name" value="ALP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000017">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF9">
        <property type="entry name" value="DUF229 DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036968.2"/>
        </source>
    </evidence>
    <sequence length="749" mass="86545" checksum="3CEF4BC9227467F8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAI2</accession>
    <name>A0A6I8VAI2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Monocarboxylate transporter 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">kar</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037203.2">
        <property type="nucleotide sequence ID" value="XM_015181717.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAI2"/>
    <dbReference type="FunCoup" id="A0A6I8VAI2">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VAI2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VAI2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000413">
        <property type="entry name" value="Karmoisin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF312">
        <property type="entry name" value="KARMOISIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015037203.2"/>
        </source>
    </evidence>
    <sequence length="629" mass="65952" checksum="C1D70F3ABB2A1823" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VB87</accession>
    <name>A0A6I8VB87_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">S-phase kinase-associated protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC26534199</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037562.1">
        <property type="nucleotide sequence ID" value="XM_015182076.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037563.1">
        <property type="nucleotide sequence ID" value="XM_015182077.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VB87"/>
    <dbReference type="GeneID" id="26534199"/>
    <dbReference type="KEGG" id="dpo:26534199"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0273186">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="2">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015037562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015037563.1"/>
        </source>
    </evidence>
    <sequence length="104" mass="11448" checksum="C037876300D9EF4B" modified="2020-08-12" version="1">MSNNEGTKMPTIKLQSSNGEDFDVDVRFLKCSGIMKGLLEDGDKEDKKKEPLVLPKVNSEILRLVMIWAEYHKDDPELPMGGPPMTSFPGTSSSSRACFGSVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VD89</accession>
    <name>A0A6I8VD89_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Y+L amino acid transporter 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC6896888</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015038858.2">
        <property type="nucleotide sequence ID" value="XM_015183372.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038859.2">
        <property type="nucleotide sequence ID" value="XM_015183373.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VD89"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VD89">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000003">
        <property type="entry name" value="Y+L amino acid transporter 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF531">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="318"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015038858.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015038859.2"/>
        </source>
    </evidence>
    <sequence length="510" mass="55755" checksum="75C4C5C1BA6C307E" modified="2020-08-12" version="1">METESLNRKNSSRKSSIVNGNGDASTKLTNGDADGGDGGDGGGGEVTLKAKMSLLNGCTVIVGSIIGSGIFVSPTGVLMYTGSVNLALIVWVASGLFSMVGAYCYAELGTMITKSGADYAYIMETFGPFMAFIRLWIECMIVRPCSQAIVALTFSTYVLKPFFPECSPPEDSARLLAVCCILVLTLINCWDVKWATAVQDIFTYAKLLALFIIIATGMYQLSLGNVQYFTFDNTDTRVTSLALSFYSGLFAYNGWNYLNFIIEELKDPVKNLPRAIAISCTLVTIVYVMANVSFYTILSPDEVMGSSAVAVTYAERAFGALAWTIPVFVALSTFGAVNGILLTSSRLFYAGANEGQMPEILTMIQIQRFTPTPAVLAMALLSMLYLTVSDIFALINYVGFATWLSIGVSVLCLPWLRWAQPNLPRPIRVPLVFPIVYLLATVFVTVVPMYASPVETGYGILMILSSIPVYLVFIAWKNKPIWFQKFMVSITHFLQKLLMVLGRQTKPAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VH93</accession>
    <name>A0A6I8VH93_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">HUWE1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        <text evidence="11">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
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    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015041340.2"/>
        </source>
    </evidence>
    <sequence length="5499" mass="586165" checksum="292C540CF592797B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJG4</accession>
    <name>A0A6I8VJG4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042608.2">
        <property type="nucleotide sequence ID" value="XM_015187122.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VJG4"/>
    <dbReference type="InParanoid" id="A0A6I8VJG4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VJG4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015042608.2"/>
        </source>
    </evidence>
    <sequence length="98" mass="10624" checksum="F2C657B7077F9983" modified="2020-08-12" version="1">MDPAESSCICQQHPCCQNQSGTETATECGILARGQRLGNAHNRRRATDHPASDADGTVPCAEASSQTVLWRRRLYCDGVLDARRGPESAIGFGVLFYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLW8</accession>
    <name>A0A6I8VLW8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lon protease homolog, mitochondrial</fullName>
            <ecNumber evidence="11">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Lon</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer or homoheptamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="11"/>
    <dbReference type="RefSeq" id="XP_015044038.2">
        <property type="nucleotide sequence ID" value="XM_015188552.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLW8"/>
    <dbReference type="FunCoup" id="A0A6I8VLW8">
        <property type="interactions" value="1253"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VLW8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VLW8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070407">
        <property type="term" value="P:oxidation-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000043">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000002">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000015">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03120">
        <property type="entry name" value="lonm_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027503">
        <property type="entry name" value="Lonm_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="16">
        <location>
            <begin position="96"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="15">
        <location>
            <begin position="809"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="220"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1010"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1028"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="911"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="954"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="573"/>
            <end position="580"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015044038.2"/>
        </source>
    </evidence>
    <sequence length="1041" mass="116249" checksum="B32132805C161731" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VN50</accession>
    <name>A0A6I8VN50_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 16">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="16">ATase</shortName>
            <ecNumber evidence="3 16">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 16">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">Prat</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:14907"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="18">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="19">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 16">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 16"/>
    <dbReference type="RefSeq" id="XP_033232452.1">
        <property type="nucleotide sequence ID" value="XM_033376561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VN50"/>
    <dbReference type="FunCoup" id="A0A6I8VN50">
        <property type="interactions" value="1486"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802993"/>
    <dbReference type="InParanoid" id="A0A6I8VN50"/>
    <dbReference type="OMA" id="PINSGHA"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000047">
        <property type="entry name" value="Amidophosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 19" id="KW-0408">Iron</keyword>
    <keyword evidence="12 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="20">
        <location>
            <begin position="49"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="529"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="532"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_033232452.1"/>
        </source>
    </evidence>
    <sequence length="547" mass="58988" checksum="83C18DF814631E02" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VP46</accession>
    <name>A0A6I8VP46_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232539.1">
        <property type="nucleotide sequence ID" value="XM_033376648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VP46"/>
    <dbReference type="KEGG" id="dpo:117183276"/>
    <dbReference type="InParanoid" id="A0A6I8VP46"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052560">
        <property type="entry name" value="RdDP_mobile_element"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36688">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36688:SF2">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232539.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="49273" checksum="4362A573141F15AE" modified="2020-08-12" version="1">DHTAVSLEGQERSLRICSAYMGHEQPDPPPHAPLRALIADCSAKDIGLIVGCDANAHHCQWGSTDTNERGEYLFSYLLTTQMVLLNRGSDPTLIIKNRKEVLDLTLASHEIQRNIVSWRVLEEHSFSDHRYVETVFSFSVSKPVQFRNIRRTNWTRYSDYLCRVLPEPPSEEEFSTEATTRLLKIFTDACNKALDKACPSGKNRGRKKPEWWNPKLGELRKASRRLFNKAAENVEQNWAEYKASLSTYNKELRIAKRASWRKFCSEIESNSEASRLRRVLSKTTPTLGYLKNTDQSWTTSSEESLNLLLNTHFPGCDENRPNYLAPPSVASNAILRLLSQENISWAIRSFKPYKSAGPDGIFPAQLIHAGHKAINWLKIIYEGIFSHGCIPDTWLQTKVVFIPKAGKPSHTAPNDFRPISLSSFLLKAMERLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQ21</accession>
    <name>A0A6I8VQ21_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor kayak, isoforms D/sro-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
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            <begin position="1"/>
            <end position="36"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233146.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="25023" checksum="D79BC4C54FE96D19" modified="2020-08-12" version="1">MIAIKSIEMQHNNNSGSIGGSGNDSHVQQQQQQQMLHQQQQQFQQDFVWANIGNISGSNSPISNSLQLQLQQQQQRSASWTAECNKQRQITNNNNNMNVNYNQHLTQQQQQQLQQYMQPTYNNYTQLQQQQQQPQQLVPATTSQSNNCYYACQQQQQQQHQQQQHQHQQHQQQQQQQFLAPTTTPSAVAATAIAATRQVIQRHVSRAKQLPLCHTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSE0</accession>
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        <submittedName>
            <fullName evidence="7 8 9">Midnolin homolog isoform X1</fullName>
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        <name evidence="7 8 9" type="primary">LOC4804180</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 9">MV-25-SWS-2005</strain>
            <strain evidence="6">MV2-25</strain>
            <tissue evidence="7 9">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="8">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033378088.1"/>
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        <property type="nucleotide sequence ID" value="XM_033378089.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033378090.1"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="CLUMA_CG009069, isoform A"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="zf-C3HC4_3"/>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="73"/>
            <end position="119"/>
        </location>
    </feature>
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            <begin position="187"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="323"/>
            <end position="357"/>
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        <location>
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            <end position="570"/>
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        <location>
            <begin position="209"/>
            <end position="255"/>
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            <begin position="261"/>
            <end position="273"/>
        </location>
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        <location>
            <begin position="276"/>
            <end position="292"/>
        </location>
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        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
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            <begin position="336"/>
            <end position="345"/>
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        <location>
            <begin position="497"/>
            <end position="507"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="508"/>
            <end position="522"/>
        </location>
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        <location>
            <begin position="523"/>
            <end position="547"/>
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        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033233979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033233980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033233981.1"/>
        </source>
    </evidence>
    <sequence length="624" mass="66110" checksum="B557B7DA69A46827" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXC0</accession>
    <name>A0A6I8VXC0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein kek2</fullName>
        </submittedName>
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        <name evidence="9 10" type="primary">kek2</name>
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    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="9 10">MV-25-SWS-2005</strain>
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        <property type="nucleotide sequence ID" value="XM_033379722.1"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="GH11366"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GL19312"/>
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        <property type="entry name" value="Leucine-rich repeat-containing 24"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Cys-rich_flank_reg_C"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF151">
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    </dbReference>
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        <property type="entry name" value="Ig_3"/>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
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    <feature type="chain" id="PRO_5044634571" evidence="6">
        <location>
            <begin position="18"/>
            <end position="912"/>
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        <location>
            <begin position="256"/>
            <end position="353"/>
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        <location>
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        <location>
            <begin position="826"/>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356502.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033235613.1"/>
        </source>
    </evidence>
    <sequence length="912" mass="98818" checksum="75DC6C074B903509" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXC6</accession>
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        <submittedName>
            <fullName evidence="3">Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC4816986</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_033235224.1">
        <property type="nucleotide sequence ID" value="XM_033379333.1"/>
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        <property type="interactions" value="1479"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816986"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032451">
        <property type="term" value="F:demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070988">
        <property type="term" value="P:demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032857">
        <property type="entry name" value="ALKBH4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12463:SF0">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12463">
        <property type="entry name" value="OXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235224.1"/>
        </source>
    </evidence>
    <sequence length="304" mass="34964" checksum="7AAFC69C3A8EE8FF" modified="2020-08-12" version="1">MNTIRPCGCKGVRTCLSCEKDFQIAKPSLQEQFQQLEPWSYCVQCERLYSGWDTTAVQADHQHHDKAKGLPMPGVLVQEQFLSKDEGSQLMADLDALRWDISQSGRRKQNFGPKTNFKKRKLQVGTFAGFPNSTEYVQRRFNEVPLLRNFQTIEQCSLEYEPSKGASIDPHVDDCWIWGERVVTVNCLGDAVLTLTVYEEQQAGKYNLDLVAAYENELLEPLLPEERLSAYKGKVLRIPMPNLSLIVLYGPARYQFEHSVLREDVVERRVCIAYREFTPMYIAGADQEKGDPVREKSQIFWQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXU7</accession>
    <name>A0A6I8VXU7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein CG13380-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6903554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235877.1">
        <property type="nucleotide sequence ID" value="XM_033379986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXU7"/>
    <dbReference type="KEGG" id="dpo:6903554"/>
    <dbReference type="InParanoid" id="A0A6I8VXU7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033235877.1"/>
        </source>
    </evidence>
    <sequence length="142" mass="16606" checksum="D7E46CAA123CD3EE" modified="2020-08-12" version="1">MEKGTDQNANFTRLNSFYVNLNGASLRDVSYRCPARKILGQKNNMQVRKYPFLSAENADVESKSDIVKKCICERPMKRLECSRCRRHFRGRVATICEKHPMETYLMDFRQCPYCSASRDKIKESDLTWDPRSEIRKAPDDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXW2</accession>
    <name>A0A6I8VXW2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Condensin complex subunit 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC117184028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CND1 (condensin subunit 1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235892.1">
        <property type="nucleotide sequence ID" value="XM_033380001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXW2"/>
    <dbReference type="KEGG" id="dpo:117184028"/>
    <dbReference type="InParanoid" id="A0A6I8VXW2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VXW2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007673">
        <property type="entry name" value="Condensin_cplx_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024324">
        <property type="entry name" value="Condensin_cplx_su1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF2">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12922">
        <property type="entry name" value="Cnd1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017127">
        <property type="entry name" value="Condensin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 N-terminal" evidence="6">
        <location>
            <begin position="80"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="442"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="948"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033235892.1"/>
        </source>
    </evidence>
    <sequence length="991" mass="114422" checksum="226939E02E4C2CB1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXY5</accession>
    <name>A0A6I8VXY5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Spidroin-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235960.1">
        <property type="nucleotide sequence ID" value="XM_033380069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXY5"/>
    <dbReference type="KEGG" id="dpo:6902578"/>
    <dbReference type="InParanoid" id="A0A6I8VXY5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250202">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026229057" evidence="2">
        <location>
            <begin position="21"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="709"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235960.1"/>
        </source>
    </evidence>
    <sequence length="942" mass="84858" checksum="D8252E6524AADE39" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VY44</accession>
    <name>A0A6I8VY44_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enolase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Eno</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="RefSeq" id="XP_033235898.1">
        <property type="nucleotide sequence ID" value="XM_033380007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VY44"/>
    <dbReference type="FunCoup" id="A0A6I8VY44">
        <property type="interactions" value="843"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817507"/>
    <dbReference type="InParanoid" id="A0A6I8VY44"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0023296">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="14">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="13">
        <location>
            <begin position="142"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="371"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033235898.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="46655" checksum="C9D2C4618BAA2651" modified="2020-08-12" version="1">MTIKAIKARQIYDSRGNPTVEVDLTTENGLFRAAVPSGASTGVHEALELRDNDKANYHGKSVLKAVAHVNDTLGPEVIKANLDVVDQAAVDNFMIKLDGTENKSKFGANAILGISLAVAKAGAAKKNVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPTGATTFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALVLITDAIAKAGYTGKIEIGMDVAASEFFKDGLYDLDFKNPASDKAQWLSPDKLANLYQEFIKEFPITSIEDPFDQDHWDAWTNLTCNTKIQIVGDDLTVTNPKRIATAVEKGACNCLLLKVNQIGTVTESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQILRIEEEIGSGVKFAGKNFRKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0Y7</accession>
    <name>A0A6I8W0Y7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Facilitated trehalose transporter Tret1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6903016</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033237020.1">
        <property type="nucleotide sequence ID" value="XM_033381129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0Y7"/>
    <dbReference type="FunCoup" id="A0A6I8W0Y7">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903016"/>
    <dbReference type="InParanoid" id="A0A6I8W0Y7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000218">
        <property type="entry name" value="facilitated trehalose transporter Tret1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF33">
        <property type="entry name" value="AT22075P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="18"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033237020.1"/>
        </source>
    </evidence>
    <sequence length="457" mass="50480" checksum="0417CFCE7A20B5F8" modified="2020-08-12" version="1">MTNICQNSLLQPKTRFQLLATVIVNIIMFGHGVGVGWLSPTLTKIQTPDSPLDFAVNIDEISWLGSMLGLGSLCGNLTMALLLERAGRKFCIYLIAGPNVCLWILIYCASNVGYLYAARFLCGFTGGAGYVVIPIFISEVADSSIRGALSSMLMLSVNLGILAGYILSTYLAYHIVPYLGILLPISYFLANFLLPETPPYLLKRSQLSAAEQSFRYYRNQQTGETSKAQFEELRTAILAQQVQNKTSLSYRDLTTRPALKAFGAAAVLCMGYQFSGVFSFINYMSDIFASSGSVLDVNTCTIIIGVVQIFGVYTSTIFVDIVGRRLLMLMSTLGVGIVCIAFGCFTHFAQIYDLSEFNWLPLALMILIVYLANIGLIGIFFLVLVELFPSKIRSLATSISVIFLSILVFCTLKLFPLFLHFWGISITMWFSAISALLTFVYFLLFLSETKGKSMIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W102</accession>
    <name>A0A6I8W102_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC26532737</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036964.2">
        <property type="nucleotide sequence ID" value="XM_015181478.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237035.1">
        <property type="nucleotide sequence ID" value="XM_033381144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W102"/>
    <dbReference type="KEGG" id="dpo:26532737"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16021">
        <property type="entry name" value="ALP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000017">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF9">
        <property type="entry name" value="DUF229 DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634592" evidence="2">
        <location>
            <begin position="19"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036964.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033237035.1"/>
        </source>
    </evidence>
    <sequence length="675" mass="78582" checksum="B3D727677ED4373A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1A7</accession>
    <name>A0A6I8W1A7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Synaptotagmin-7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Syt7</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237063.1">
        <property type="nucleotide sequence ID" value="XM_033381172.1"/>
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        <property type="interactions" value="35"/>
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    <dbReference type="KEGG" id="dpo:6899894"/>
    <dbReference type="InParanoid" id="A0A6I8W1A7"/>
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        <property type="component" value="Chromosome 5"/>
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    <dbReference type="Bgee" id="FBgn0243916">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calcium-dependent phospholipid binding"/>
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        <property type="term" value="F:phosphatidylserine binding"/>
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        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:vesicle fusion"/>
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    </dbReference>
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        <property type="entry name" value="synaptotagmin-7 isoform X1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
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        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF344">
        <property type="entry name" value="SYNAPTOTAGMIN-7"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00168">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="178"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="307"/>
            <end position="442"/>
        </location>
    </feature>
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        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033237063.1"/>
        </source>
    </evidence>
    <sequence length="447" mass="50777" checksum="CDC093C092E6E6BE" modified="2020-08-12" version="1">MYVKIFVHIYQVKNICFHFKKGHQIYDFFLIADMASIILIACLAILGLIIAIALFLAGGYLWWRHKRSQLQFIEPNEDEESSSYSLRAAQDIDSGNPPSKPQVPVAQAITTPLQNNINRKLNGFLSLRTPLIGGSAPAQAKAQNASTGNTNDGTSKDSANKSISMTDMYLDSTDSSENVGQIHFSLEYDFQNTTLILKVLQGKELPAKDLSGTSDPYVRVTLLPDKKHRLETKIKRRTLNPRWNETFYFEGFPIQKLQSRVLHLHVFDYDRFSRDDSIGEVFLPLCQVDFAGKQSFWKALKPPAKDKCGELLSSLCYHPSNSILTLTLIKARNLKAKDINGKSDPYVKVWLQFGDKRVEKRKTPIFTCTLNPVFNESFSFNVPWEKIRECSLDVMVMDFDNIGRNELIGRILLAGKNGSGASETKHWQDMISKPRQTVVQWHRLKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4F2</accession>
    <name>A0A6I8W4F2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8 9">Nudix hydrolase 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8 9" type="primary">LOC6902260</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8 9">MV-25-SWS-2005</strain>
            <tissue evidence="7 8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133787.2">
        <property type="nucleotide sequence ID" value="XM_002133751.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238202.1">
        <property type="nucleotide sequence ID" value="XM_033382311.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238203.1">
        <property type="nucleotide sequence ID" value="XM_033382312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4F2"/>
    <dbReference type="KEGG" id="dpo:6902260"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04670">
        <property type="entry name" value="NUDIX_ASFGF2_Nudt6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nudix hydrolase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003293">
        <property type="entry name" value="Nudix_hydrolase6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040618">
        <property type="entry name" value="Pre-Nudix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994:SF13">
        <property type="entry name" value="FI03680P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13994">
        <property type="entry name" value="NUDIX HYDROLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18290">
        <property type="entry name" value="Nudix_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01356">
        <property type="entry name" value="GFGPROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="5">
        <location>
            <begin position="162"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002133787.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033238202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033238203.1"/>
        </source>
    </evidence>
    <sequence length="331" mass="38446" checksum="1B8D47C365A1AFC1" modified="2020-08-12" version="1">MLSTAILGRSTRLPLQAFNSWRRRLRTNRSTSSVSTSPQHQREQATHQQSRQFTRAFHSHKAMDSPPDVFRGQTDRFAGVTVDGREETIEKSQFEEKLNKSLEWWTKNKNRAIWFRVYKEQADWVPILAAAGFDFHHARTGIVVMYRWLPTHESINLPNYAHTLLGVGGLVINDKDEVLVVSDRYAMIPNSWKLPGGYVEPRENLIDAAIREVEEETGIRTTFRSVVCLRHAHGGNFGCSDIYMVIALKPLNLDFTRCEREIARLQWMPIAEYLQHPQVHETNRQFVHTFLDYQKRGLVLTCRNQVHQVLKREYNLYFVDGQSKPDDGPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4T9</accession>
    <name>A0A6I8W4T9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8 9 10">S-phase kinase-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">LOC117183193</name>
        <name evidence="9 10" type="synonym">SkpA</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8 9">MV-25-SWS-2005</strain>
            <tissue evidence="7 8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033237724.1">
        <property type="nucleotide sequence ID" value="XM_033381833.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237725.1">
        <property type="nucleotide sequence ID" value="XM_033381834.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239808.1">
        <property type="nucleotide sequence ID" value="XM_033383917.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239809.1">
        <property type="nucleotide sequence ID" value="XM_033383918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4T9"/>
    <dbReference type="SMR" id="A0A6I8W4T9"/>
    <dbReference type="KEGG" id="dpo:117183193"/>
    <dbReference type="KEGG" id="dpo:6899792"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000018">
        <property type="entry name" value="S-phase kinase-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="5">
        <location>
            <begin position="3"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="4">
        <location>
            <begin position="112"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033237724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033237725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033239808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033239809.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18638" checksum="40122A6E5C882408" modified="2020-08-12" version="1">MPNIKLQSSDEEIFDTDIQIAKCSGTIKTMLEDCGMEDDENAIVPLPNVNSTILRKVLTWAHYHKDDPQPTEDDESKEKRTDDIISWDADFLKVDQGTLFELILAANYLDIKGLLELTCKTVANMIKGKTPEEIRKTFNIKKDFTPAEEEQVRKENEWCEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WA69</accession>
    <name>A0A6I8WA69_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UPF0488 protein CG14286</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0488 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240296.1">
        <property type="nucleotide sequence ID" value="XM_033384405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WA69"/>
    <dbReference type="FunCoup" id="A0A6I8WA69">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901672"/>
    <dbReference type="InParanoid" id="A0A6I8WA69"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029274">
        <property type="entry name" value="DUF4615"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13602">
        <property type="entry name" value="UPF0488 PROTEIN C8ORF33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13602:SF2">
        <property type="entry name" value="UPF0488 PROTEIN C8ORF33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15393">
        <property type="entry name" value="DUF4615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033240296.1"/>
        </source>
    </evidence>
    <sequence length="216" mass="23785" checksum="CE9CAFCEBB2CD7C9" modified="2020-08-12" version="1">MHRRKTAKSINKPPPIQGPAKKPVAAASAATAPGFDGESAIQFELELCWCVQQLQLALDGGKLSQKVAEDTAKNLKILTSQTAPLIKKRQVMKLSMGDYRSKMQQEERKMMLASRQIKFTPSADTNKKSSFVKKSALLTSGKDFRFEFALPTAANTNENAPEPAQETASQHPQSSDLPSLRSSLRQESTGSQFKFNFTIEDDHANDINFSGLRLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WB09</accession>
    <name>A0A6I8WB09_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18 19 20">G-protein coupled receptor Mth-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18 19 20 21 22 23" type="primary">LOC26532572</name>
    </gene>
    <organism evidence="17 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18 19 20">MV-25-SWS-2005</strain>
            <tissue evidence="18 19 20">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Mth subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352477.4">
        <property type="nucleotide sequence ID" value="XM_001352441.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043605.2">
        <property type="nucleotide sequence ID" value="XM_015188119.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240593.1">
        <property type="nucleotide sequence ID" value="XM_033384702.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240595.1">
        <property type="nucleotide sequence ID" value="XM_033384704.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240596.1">
        <property type="nucleotide sequence ID" value="XM_033384705.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240597.1">
        <property type="nucleotide sequence ID" value="XM_033384706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WB09"/>
    <dbReference type="KEGG" id="dpo:26532572"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008340">
        <property type="term" value="P:determination of adult lifespan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15039">
        <property type="entry name" value="7tmB3_Methuselah-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00251">
        <property type="entry name" value="Mth_Ecto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000297">
        <property type="entry name" value="G-protein coupled receptor Mth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.180.11:FF:000001">
        <property type="entry name" value="G-protein coupled receptor Mth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.160.11:FF:000001">
        <property type="entry name" value="G-protein coupled receptor Mth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.160.11">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.180.11">
        <property type="entry name" value="Methuselah ectodomain, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044860">
        <property type="entry name" value="Methusela_ecto_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023311">
        <property type="entry name" value="Methusela_ecto_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010596">
        <property type="entry name" value="Methuselah_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036272">
        <property type="entry name" value="Methuselah_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051384">
        <property type="entry name" value="Mth_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47154">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47154:SF2">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06652">
        <property type="entry name" value="Methuselah_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63877">
        <property type="entry name" value="Methuselah ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634634" evidence="15">
        <location>
            <begin position="22"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="454"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="16">
        <location>
            <begin position="214"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001352477.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015043605.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033240593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_033240595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_033240596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_033240597.1"/>
        </source>
    </evidence>
    <sequence length="531" mass="61242" checksum="2556B5EE104F45C9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBF1</accession>
    <name>A0A6I8WBF1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">G-protein coupled receptor Mth-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">LOC26533489</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17">MV-25-SWS-2005</strain>
            <tissue evidence="16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Mth subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240601.1">
        <property type="nucleotide sequence ID" value="XM_033384710.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240602.1">
        <property type="nucleotide sequence ID" value="XM_033384711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WBF1"/>
    <dbReference type="KEGG" id="dpo:26533489"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00251">
        <property type="entry name" value="Mth_Ecto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.160.11:FF:000001">
        <property type="entry name" value="G-protein coupled receptor Mth"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.180.11">
        <property type="entry name" value="Methuselah ectodomain, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044860">
        <property type="entry name" value="Methusela_ecto_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023311">
        <property type="entry name" value="Methusela_ecto_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010596">
        <property type="entry name" value="Methuselah_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036272">
        <property type="entry name" value="Methuselah_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051384">
        <property type="entry name" value="Mth_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47154">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47154:SF2">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06652">
        <property type="entry name" value="Methuselah_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63877">
        <property type="entry name" value="Methuselah ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634636" evidence="13">
        <location>
            <begin position="20"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Methuselah N-terminal" evidence="14">
        <location>
            <begin position="29"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033240601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033240602.1"/>
        </source>
    </evidence>
    <sequence length="160" mass="17897" checksum="45D30DD850EACB16" modified="2020-08-12" version="1">MLLILSAVCVTLLNTATLAATENETIPDCDYFDTVNVTGSQRLVNGSYLYQGVVIPAELTAEFDYRVLPDDSTEPVERHLRGCVCQLRPCLRLCCHHSQLMANGQCSGSIEVDLTRLNPYLSVTLDDGTVARRHFQEEFIIQSDLPIPCNDMYPLNDQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCK5</accession>
    <name>A0A6I8WCK5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium-dependent nutrient amino acid transporter 1 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">blot</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241123.1">
        <property type="nucleotide sequence ID" value="XM_033385232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCK5"/>
    <dbReference type="FunCoup" id="A0A6I8WCK5">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WCK5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WCK5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015375">
        <property type="term" value="F:glycine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06857">
        <property type="entry name" value="SLC5-6-like_sbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF240">
        <property type="entry name" value="BLOATED TUBULES, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="534"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="565"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="634"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="670"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="764"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="837"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="766"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="539"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033241123.1"/>
        </source>
    </evidence>
    <sequence length="973" mass="106733" checksum="4F72EB36AEF3A355" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WD05</accession>
    <name>A0A6I8WD05_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vesicle transport protein USE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">USE1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Use1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the USE1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240449.1">
        <property type="nucleotide sequence ID" value="XM_033384558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WD05"/>
    <dbReference type="FunCoup" id="A0A6I8WD05">
        <property type="interactions" value="1890"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WD05"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15860">
        <property type="entry name" value="SNARE_USE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019150">
        <property type="entry name" value="Vesicle_transport_protein_Use1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050">
        <property type="entry name" value="USE1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050:SF7">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN USE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09753">
        <property type="entry name" value="Use1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033240449.1"/>
        </source>
    </evidence>
    <sequence length="248" mass="28416" checksum="5349F697CD315822" modified="2020-08-12" version="1">MATKLNVNIRVMLNNCEELAKNEANFWRLQKFIKALDIMVAELQDMDDSRSARRISGYKERLQALKLTTGYTESTKIQTNEVEAAEVSLKEMRQIESTKNYNHMRKDLLEVDGDALRRRRGVAEDAGGSGSPSGSSNSPATEGDNLNEAVKYYNQKQEKITEHMLSLTRNLKEQTMTANRIIKRDTELVTKSSGMADRNINSLGKEVETLEQHSRSAYKCWAWMLFVFVFCTFVGMVFFMKIMKKKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKC2</accession>
    <accession>A0A6I8UYJ5</accession>
    <name>B5DKC2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTP-binding nuclear protein Ran-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6902412</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133346.2">
        <property type="nucleotide sequence ID" value="XM_002133310.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKC2"/>
    <dbReference type="SMR" id="B5DKC2"/>
    <dbReference type="STRING" id="46245.B5DKC2"/>
    <dbReference type="GeneID" id="6902412"/>
    <dbReference type="KEGG" id="dpo:6902412"/>
    <dbReference type="eggNOG" id="KOG0096">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_13_0_1"/>
    <dbReference type="InParanoid" id="B5DKC2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249386">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DKC2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002133346.2"/>
        </source>
    </evidence>
    <sequence length="203" mass="23276" checksum="A33558E6DE270018" modified="2016-01-20" version="2">MRRQGKVENVSQEKSNLSNCRARTMDVPTYKCIIMGEKCCGKTTFLKRHLTGEFVEEYVHTARGSTSIVTLTFNSNRGPIQFDVFDWGQENEELLLHPEDFYKDGQCAIVMFDVSSVFIFGDFFKQFLMLNLPISLCGNKADIKTDNYSVYDPENFLSSPNLEKKIFSEISVKTGLNIDQPFLYLARKLLNDFTLEFIPSTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>B5DLL1</accession>
    <accession>A0A6I8UZ19</accession>
    <name>B5DLL1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RPB5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6902259</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively. In RNA Pol II, this subunit is present in 2-fold molar excess over the other subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133786.1">
        <property type="nucleotide sequence ID" value="XM_002133750.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DLL1"/>
    <dbReference type="FunCoup" id="B5DLL1">
        <property type="interactions" value="2036"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DLL1"/>
    <dbReference type="GeneID" id="6902259"/>
    <dbReference type="KEGG" id="dpo:6902259"/>
    <dbReference type="eggNOG" id="KOG3218">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058320_0_1_1"/>
    <dbReference type="InParanoid" id="B5DLL1"/>
    <dbReference type="OMA" id="VRDRGYF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244005">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DLL1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1340.10:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="10">
        <location>
            <begin position="137"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002133786.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="24470" checksum="0EEE3815AB46F328" modified="2008-10-14" version="1">MDDEAETYKLWRIRKTIMQLSHDRGYLVTQDELDQTLEQFKDMFGDKPSEKRPARSDLIVLVAHNDDPTDQMFVFFPEEPKIGIKTIKTYCTRMQEENIHRAIVVVQGGMTPSAKQSLVDMAPKYILEQFLESELLINITEHELVPEHVVMTVEEKQELLSRYKLKENMLMRIQAGDPVARYFGLKRGQVVKIIRSSETAGRYISYRLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>B5DLN4</accession>
    <accession>A0A6I8UZ21</accession>
    <name>B5DLN4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6902282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133809.2">
        <property type="nucleotide sequence ID" value="XM_002133773.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DLN4"/>
    <dbReference type="STRING" id="46245.B5DLN4"/>
    <dbReference type="GeneID" id="6902282"/>
    <dbReference type="KEGG" id="dpo:6902282"/>
    <dbReference type="HOGENOM" id="CLU_1134589_0_0_1"/>
    <dbReference type="InParanoid" id="B5DLN4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244491">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DLN4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002133809.2"/>
        </source>
    </evidence>
    <sequence length="131" mass="14940" checksum="7285E7D15AAD757B" modified="2016-01-20" version="2">MSKLAQSHFQSPRFSTLHMKADYTYTPFLKKPIHLSNVTLEHGIWAKLWLKTKSNEKMLADLRECSPKVSLDIDIHPGGVLYLESNGPVFVTLVGCMRELEHGKGAFRMEDCFITATKWVKCEVQNNNPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>B5DMT4</accession>
    <accession>A0A6I8W771</accession>
    <name>B5DMT4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Mitochondrial import inner membrane translocase subunit Tim16-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC6901823</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239198.1">
        <property type="nucleotide sequence ID" value="XM_033383307.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239199.1">
        <property type="nucleotide sequence ID" value="XM_033383308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DMT4"/>
    <dbReference type="STRING" id="46245.B5DMT4"/>
    <dbReference type="KEGG" id="dpo:6901823"/>
    <dbReference type="eggNOG" id="KOG3442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101461_3_0_1"/>
    <dbReference type="OMA" id="QHLFRAN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245524">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DMT4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000006">
        <property type="entry name" value="Import inner membrane translocase subunit TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014300068" evidence="9">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033239198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033239199.1"/>
        </source>
    </evidence>
    <sequence length="127" mass="14343" checksum="AA113FA3CC690B47" modified="2008-10-14" version="1">MARYLVRIVLLGAQSIGKAFAKTVRQEVEVYREAARVHAAQSATFNPTKTDAALKGMTLEEAQLILNVKDVLDRGEVENKFQYLFHANQKKAGGSFYIQSKVVRAKERIDQEIKKWEAQTVSQSESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>B5DQU9</accession>
    <accession>A0A6I8V0T4</accession>
    <name>B5DQU9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6899899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135267.1">
        <property type="nucleotide sequence ID" value="XM_002135231.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DQU9"/>
    <dbReference type="GeneID" id="6899899"/>
    <dbReference type="KEGG" id="dpo:6899899"/>
    <dbReference type="eggNOG" id="ENOG502T8MR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1316655_0_0_1"/>
    <dbReference type="InParanoid" id="B5DQU9"/>
    <dbReference type="OMA" id="FYCDGQN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249800">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DQU9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002135267.1"/>
        </source>
    </evidence>
    <sequence length="209" mass="23969" checksum="AF22C251FCCACF83" modified="2008-10-14" version="1">MPVGSQCAQRLYVKRIDCSRLHLLLVLQLLLLLLLLGRPIPVQSANDNCTFIDGYVLQYLCQGRFDREMRLMYKDRIPSMDAPYTIWQRSDTISPVYLLVAFYNSPISSCFTLVMDGGKFYCDGQNYVMKLHTSAEVHCLAFPFDYAEMLYESCMKEEYPSDTEPIAVAIIYMKNNIIKIIESGPDLKASAVLVLFSALGCWTSPFWYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>B5DQY7</accession>
    <accession>A0A6I8V181</accession>
    <name>B5DQY7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nitrilase and fragile histidine triad fusion protein NitFhit</fullName>
            <ecNumber evidence="3">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NitFhit</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cleaves A-5'-PPP-5'A to yield AMP and ADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the UPF0012 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002135347.2">
        <property type="nucleotide sequence ID" value="XM_002135311.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DQY7"/>
    <dbReference type="SMR" id="B5DQY7"/>
    <dbReference type="FunCoup" id="B5DQY7">
        <property type="interactions" value="645"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DQY7"/>
    <dbReference type="GeneID" id="6899980"/>
    <dbReference type="KEGG" id="dpo:6899980"/>
    <dbReference type="eggNOG" id="KOG0807">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_12_1_1"/>
    <dbReference type="InParanoid" id="B5DQY7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249746">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DQY7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000011">
        <property type="entry name" value="Fragile histidine triad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000005">
        <property type="entry name" value="nitrilase homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="11">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="408"/>
            <end position="411"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for induction of apoptosis" evidence="13">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002135347.2"/>
        </source>
    </evidence>
    <sequence length="465" mass="52585" checksum="2524C02B838D2A0B" modified="2016-01-20" version="2">MSTLVKIIRQSISSQNRQQQLRKMASGTEKIINGQSATIAVGQMRSTNDKVWNLNQVKELIKKAKSENAQMLFLPECCDFVGESRTQTIELAERLDGKLVAEYKELARCHQIWLSLGGIHELNDQGKIYNAHVLVSAKGELAGVYRKMHLFDATTKEIRLRESDTVSPGERLERPVATPAGQVGLQICYDLRFAEPAVLLRKLGAQILTYPAAFTYATGKAHWEILLRARAIETQCFVIAAAQQGWHNQKRQSWGHSMIISPWGKVLADCGGEKDLDIGTAKIDLSTLDSLYQSMPCFEHRRNDLYSLTAYNIRSREPDTDRAFAEHMVDKRTIFYESEHCFAFTNLRCVVEGHVLVSTKRVTPRLCGLDCAEITDMFATVCMVQRLLEKIYQTTSATVTVQDGAQAGQTVPHVHFHVMPRRKGDFGHNDQIYVKLEDCTESKPPRTLQERIDEAQVYRTYLSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DT4</accession>
    <accession>A0A6I8UBD7</accession>
    <name>Q29DT4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidoglycan-recognition protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PGRP-SB1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352829.2">
        <property type="nucleotide sequence ID" value="XM_001352793.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DT4"/>
    <dbReference type="SMR" id="Q29DT4"/>
    <dbReference type="FunCoup" id="Q29DT4">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DT4"/>
    <dbReference type="GeneID" id="4812163"/>
    <dbReference type="KEGG" id="dpo:4812163"/>
    <dbReference type="eggNOG" id="ENOG502QR3D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037559_3_2_1"/>
    <dbReference type="InParanoid" id="Q29DT4"/>
    <dbReference type="OMA" id="QTDHKGR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081946">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DT4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.80.10:FF:000001">
        <property type="entry name" value="Peptidoglycan recognition protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017331">
        <property type="entry name" value="Peptidoglycan_recognition"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF75">
        <property type="entry name" value="PEPTIDOGLYCAN-RECOGNITION PROTEIN SB1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037945">
        <property type="entry name" value="PGRPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00701">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 8" id="KW-0391">Immunity</keyword>
    <keyword evidence="4 8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="10">
        <location>
            <begin position="23"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="9">
        <location>
            <begin position="34"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001352829.2"/>
        </source>
    </evidence>
    <sequence length="188" mass="20482" checksum="FA38AAA87F5DB73D" modified="2008-10-14" version="2">MTTNKFLAALMALCAIALSAEALNIEPRSSWGAVAARSPARISGAVDYVIIHHSDNPNGCSTSDQCQRMIKAIQTDHKGRRSFSDIGYNFIVAGDGKVYEGRGFGLQGSHAPNYNRNSIGIVFIGNFESTKPSSQMLQNAKDLIDKAKQGGYLKNTYTLLGHRQTKATACPGTALFNEIKTWPNWRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EC6</accession>
    <accession>A0A6I8UB02</accession>
    <name>Q29EC6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Acetylcholine receptor subunit beta-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">nAChRbeta1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352637.2">
        <property type="nucleotide sequence ID" value="XM_001352601.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EC6"/>
    <dbReference type="STRING" id="46245.Q29EC6"/>
    <dbReference type="GeneID" id="4811870"/>
    <dbReference type="KEGG" id="dpo:4811870"/>
    <dbReference type="eggNOG" id="KOG3645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018074_1_0_1"/>
    <dbReference type="InParanoid" id="Q29EC6"/>
    <dbReference type="OMA" id="RWMMEIP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070993">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EC6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19032">
        <property type="entry name" value="LGIC_ECD_nAChR_proto_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000035">
        <property type="entry name" value="Acetylcholine receptor subunit beta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000038">
        <property type="entry name" value="Acetylcholine receptor subunit beta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000023">
        <property type="entry name" value="Acetylcholine receptor subunit beta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="18">
        <location>
            <begin position="29"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="19">
        <location>
            <begin position="244"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001352637.2"/>
        </source>
    </evidence>
    <sequence length="522" mass="59891" checksum="40C71DB684CA1F87" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EE4</accession>
    <accession>A0A6I8UAZ9</accession>
    <name>Q29EE4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proton-coupled amino acid transporter-like protein pathetic isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">path</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352619.1">
        <property type="nucleotide sequence ID" value="XM_001352583.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EE4"/>
    <dbReference type="FunCoup" id="Q29EE4">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EE4"/>
    <dbReference type="GeneID" id="4812542"/>
    <dbReference type="KEGG" id="dpo:4812542"/>
    <dbReference type="eggNOG" id="KOG1304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_0_1_1"/>
    <dbReference type="InParanoid" id="Q29EE4"/>
    <dbReference type="OMA" id="MYSGLVM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077456">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EE4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF154">
        <property type="entry name" value="PROTON-COUPLED AMINO ACID TRANSPORTER-LIKE PROTEIN PATHETIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="5">
        <location>
            <begin position="61"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001352619.1"/>
        </source>
    </evidence>
    <sequence length="469" mass="51918" checksum="369F9B6D72F7B46A" modified="2006-04-04" version="1">MVNIVDGGAKHAPQEMEQFLPGEGGKTYHIQPRKADNEQALGNNEFDPFAHRDNEHPTSDNETLTHLLKASLGTGILGMPIAFMYSGIIMGIFATIFTAFVCTHCSYVLVKCGHKLYYKTRRTKMTFAEIAESAFQKGPKWSRGFAPVAKFSILFGLFLTYFGTCSVYTVIVAKNFEQLIEHWTGTEVSSRLLICSLLVPLILIAWVPNLKYLAPVSMVANVFMGLGLGITFYYLTQDLPPLESRNYLVLGTLPSFFSITIFAMEAIGVVMPLENNMKTPQNFLGLCGVLSQGMSGVTLIYMLLGFLGYLRYGEDTQQSITLNLPVHEWPAQAVKVLIALAVYCTFGLQFYVCLEIVWDGIKEKCTKRPMLVNYVLRTVLVTAAVVLAISVPTIAPFMGLIGAFCFSILGLIFPVLIELVLHWDTGFGKYNWILWKNIVICICGIGALVFGSLAAIRDIVEVYTAVAPQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-17" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EP6</accession>
    <name>JIP3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>JNK-interacting protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein sunday driver</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">syd</name>
        <name type="ORF">GA20831</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK-signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Syd is required for efficient kinesin-I mediated axonal transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms homo- and heterooligomeric complexes. Binds the TPR motif-containing C-terminal of kinesin light chain, Klc. Pre-assembled syd scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379070">
        <property type="protein sequence ID" value="EAL30014.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001352517.2">
        <property type="nucleotide sequence ID" value="XM_001352481.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EP6"/>
    <dbReference type="SMR" id="Q29EP6"/>
    <dbReference type="FunCoup" id="Q29EP6">
        <property type="interactions" value="1477"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EP6"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0288268">
        <property type="protein sequence ID" value="FBpp0286706"/>
        <property type="gene ID" value="FBgn0080821"/>
    </dbReference>
    <dbReference type="GeneID" id="4812552"/>
    <dbReference type="KEGG" id="dpo:4812552"/>
    <dbReference type="CTD" id="43905"/>
    <dbReference type="eggNOG" id="KOG2077">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003841_0_0_1"/>
    <dbReference type="InParanoid" id="Q29EP6"/>
    <dbReference type="OMA" id="WEVDAEL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080821">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030140">
        <property type="term" value="C:trans-Golgi network transport vesicle"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008088">
        <property type="term" value="P:axo-dendritic transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1000:FF:000001">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1000">
        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039911">
        <property type="entry name" value="JIP3/JIP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032486">
        <property type="entry name" value="JIP_LZII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034743">
        <property type="entry name" value="RH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034744">
        <property type="entry name" value="RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886:SF4">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886">
        <property type="entry name" value="JNK/SAPK-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16471">
        <property type="entry name" value="JIP_LZII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09744">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51776">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51777">
        <property type="entry name" value="RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000284449" description="JNK-interacting protein 3">
        <location>
            <begin position="1"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="domain" description="RH1" evidence="5">
        <location>
            <begin position="25"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="RH2" evidence="6">
        <location>
            <begin position="456"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="278"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="520"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="813"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="869"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="84"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="366"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="529"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="544"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="874"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q58A65"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q9GQF1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="1235" mass="137449" checksum="55CE2D02184A5465" modified="2009-02-10" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HZ4</accession>
    <accession>A0A6I8UF74</accession>
    <name>Q29HZ4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Single domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4814805</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354559.2">
        <property type="nucleotide sequence ID" value="XM_001354523.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HZ4"/>
    <dbReference type="FunCoup" id="Q29HZ4">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HZ4"/>
    <dbReference type="GeneID" id="4814805"/>
    <dbReference type="KEGG" id="dpo:4814805"/>
    <dbReference type="eggNOG" id="ENOG502TKTS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1929732_0_0_1"/>
    <dbReference type="InParanoid" id="Q29HZ4"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073603">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HZ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="SVWC_dom"/>
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    <dbReference type="InterPro" id="IPR053308">
        <property type="entry name" value="Vago-like"/>
    </dbReference>
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        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Single" evidence="3">
        <location>
            <begin position="41"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354559.2"/>
        </source>
    </evidence>
    <sequence length="123" mass="13691" checksum="FC47456FC42C79EE" modified="2008-10-14" version="2">MFQDRYTHFLAWLAIGGALLMAQVNAYSGLIPPDPSNPGRCTYRGDVLESGVNNGISPCQRLTCNEDGSILIEGCGKLRIENCNRGERIYHGKPFPECCKLKYKCKQIGAAPYYIERDTAEPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IT3</accession>
    <accession>A0A6I8UHD2</accession>
    <name>Q29IT3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin-related protein 5, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Monothiol glutaredoxin-5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Grx5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355511.2">
        <property type="nucleotide sequence ID" value="XM_001355475.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IT3"/>
    <dbReference type="SMR" id="Q29IT3"/>
    <dbReference type="FunCoup" id="Q29IT3">
        <property type="interactions" value="1477"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IT3"/>
    <dbReference type="GeneID" id="4815995"/>
    <dbReference type="KEGG" id="dpo:4815995"/>
    <dbReference type="eggNOG" id="KOG0911">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_2_0_1"/>
    <dbReference type="InParanoid" id="Q29IT3"/>
    <dbReference type="OMA" id="YEVLDEP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072997">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IT3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000005">
        <property type="entry name" value="Glutaredoxin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="62"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355511.2"/>
        </source>
    </evidence>
    <sequence length="171" mass="19070" checksum="ADBFE6C3C3914B9A" modified="2008-10-14" version="2">MNRICQTLLRQSYARAPLTSNHVAATATRCTRWYSADASPPASAADVVDKSTLEKLVRTNKVVIFMKGNPQAPRCGFSNAVVQIMRMHGVQYDAHDVLQNEALRQGVKEFTDWPTIPQVFIDGEFVGGCDILMQLHQSGDLIEELKKVGIISELLKAEEAKQEPNTEEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q29K76</accession>
    <accession>A0A6I8UIW6</accession>
    <name>Q29K76_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Origin recognition complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Orc5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356239.2">
        <property type="nucleotide sequence ID" value="XM_001356203.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29K76"/>
    <dbReference type="SMR" id="Q29K76"/>
    <dbReference type="FunCoup" id="Q29K76">
        <property type="interactions" value="2929"/>
    </dbReference>
    <dbReference type="GeneID" id="4816621"/>
    <dbReference type="KEGG" id="dpo:4816621"/>
    <dbReference type="eggNOG" id="KOG2543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028223_0_1_1"/>
    <dbReference type="InParanoid" id="Q29K76"/>
    <dbReference type="OMA" id="QLRRWHG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080611">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000673">
        <property type="entry name" value="Origin recognition complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047088">
        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048866">
        <property type="entry name" value="ORC5_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21639">
        <property type="entry name" value="ORC5_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="8">
        <location>
            <begin position="49"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="ORC5 lid" evidence="10">
        <location>
            <begin position="265"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="9">
        <location>
            <begin position="360"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356239.2"/>
        </source>
    </evidence>
    <sequence length="499" mass="56653" checksum="6946C95A83011EC8" modified="2008-10-14" version="2">MIAAHYRLHAGAIGYSAGKYSHFCALYPRQLFVSKSQMENICAALEPLFPCREGAIKTLGELIGDSREDYPSALYLFGHSGTGKTALTRTFLRECSRQQGVRIAQLNAIECYTTKIMLENMLDTLAPRQCTEESVRADNMLEFVEQLRRWHDQGGRSQSFLIAVDNAERLRDMDANVLPVLLRLQQLTSLNLCVILLSQIPFEKFYNKTGLTEVISIHLAQYNKAETQRILGSDFFQVRGHLLEQFSKDVQRLAICEAALTEDFYSNYLNLFLSVFYKACRDVPELQLTARKCFSIYLEPVLDGTVESTDVSRLWRHIAGPLRSALTQIYMRTEKPPEEQEGSAPIEDQSVRKLAQSLELPYYAKYLLIAAFLASHNSANQDKRLFVKHHGKQRKRMQTVNARAKTTEKMSTTLGPKSFTIDRLLAIFYAILEEKVGLTCNLLSQISTLVHLKLLSFVSGEQNIMDGSARLQCTVGLEFVLYIGKVVGFNVRQYLCDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KB9</accession>
    <accession>A0A6I8UJ48</accession>
    <name>Q29KB9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Pgant35A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_001356196.3">
        <property type="nucleotide sequence ID" value="XM_001356160.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KB9"/>
    <dbReference type="SMR" id="Q29KB9"/>
    <dbReference type="FunCoup" id="Q29KB9">
        <property type="interactions" value="331"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KB9"/>
    <dbReference type="GeneID" id="4816770"/>
    <dbReference type="KEGG" id="dpo:4816770"/>
    <dbReference type="eggNOG" id="KOG3736">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013477_0_1_1"/>
    <dbReference type="InParanoid" id="Q29KB9"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080377">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KB9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000075">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000053">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF63">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 13" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="14">
        <location>
            <begin position="476"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001356196.3"/>
        </source>
    </evidence>
    <sequence length="618" mass="70454" checksum="A442C475E9C506E9" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KF6</accession>
    <accession>A0A6I8V9T2</accession>
    <name>Q29KF6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">uncharacterized protein LOC4816680</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10" type="primary">LOC4816680</name>
    </gene>
    <organism evidence="7 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9 10">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="8 9 10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356159.1">
        <property type="nucleotide sequence ID" value="XM_001356123.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036742.1">
        <property type="nucleotide sequence ID" value="XM_015181256.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036743.1">
        <property type="nucleotide sequence ID" value="XM_015181257.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KF6"/>
    <dbReference type="STRING" id="46245.Q29KF6"/>
    <dbReference type="GeneID" id="4816680"/>
    <dbReference type="KEGG" id="dpo:4816680"/>
    <dbReference type="eggNOG" id="KOG1609">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036656_0_0_1"/>
    <dbReference type="OMA" id="ICYDTDK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075580">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KF6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20893">
        <property type="entry name" value="LD08641P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20893:SF2">
        <property type="entry name" value="LD08641P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="387"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015036742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015036743.1"/>
        </source>
    </evidence>
    <sequence length="566" mass="63299" checksum="540580723A4521F6" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q29L36</accession>
    <accession>A0A6I8UI75</accession>
    <name>Q29L36_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Proton channel OtopLc isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">OtopLb</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355929.3">
        <property type="nucleotide sequence ID" value="XM_001355893.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29L36"/>
    <dbReference type="STRING" id="46245.Q29L36"/>
    <dbReference type="GeneID" id="4816398"/>
    <dbReference type="KEGG" id="dpo:4816398"/>
    <dbReference type="eggNOG" id="KOG4740">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011508_1_1_1"/>
    <dbReference type="InParanoid" id="Q29L36"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077398">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF30">
        <property type="entry name" value="GH01206P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355929.3"/>
        </source>
    </evidence>
    <sequence length="800" mass="89959" checksum="39EB17C0882A9B40" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LK3</accession>
    <accession>A0A6I8UKH8</accession>
    <name>Q29LK3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex I-B8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase B8 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ND-B8</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA2 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356976.1">
        <property type="nucleotide sequence ID" value="XM_001356940.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LK3"/>
    <dbReference type="SMR" id="Q29LK3"/>
    <dbReference type="FunCoup" id="Q29LK3">
        <property type="interactions" value="1182"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LK3"/>
    <dbReference type="GeneID" id="4817571"/>
    <dbReference type="KEGG" id="dpo:4817571"/>
    <dbReference type="eggNOG" id="KOG3446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110897_1_0_1"/>
    <dbReference type="InParanoid" id="Q29LK3"/>
    <dbReference type="OMA" id="FIEQQYV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073760">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LK3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016464">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12878">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B8 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005822">
        <property type="entry name" value="NDUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="13">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001356976.1"/>
        </source>
    </evidence>
    <sequence length="95" mass="10689" checksum="3314BFF78CA943BD" modified="2006-04-04" version="1">MRLTLTRLAALTPKLKELRIVLDPTGDASKGAREFVQKFYPNLKRDNPNLPIMVRECAGAQPRLWARYGKGKEISVSLTNREAPDIQKQLEAVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NI0</accession>
    <accession>A0A6I8UJ30</accession>
    <name>Q29NI0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RpL7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to G-rich structures in 28S rRNA and in mRNAs. Plays a regulatory role in the translation apparatus; inhibits cell-free translation of mRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356299.1">
        <property type="nucleotide sequence ID" value="XM_001356263.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NI0"/>
    <dbReference type="SMR" id="Q29NI0"/>
    <dbReference type="FunCoup" id="Q29NI0">
        <property type="interactions" value="1275"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NI0"/>
    <dbReference type="GeneID" id="4817486"/>
    <dbReference type="KEGG" id="dpo:4817486"/>
    <dbReference type="eggNOG" id="KOG3184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_0_2_1"/>
    <dbReference type="InParanoid" id="Q29NI0"/>
    <dbReference type="OMA" id="SYYVDAQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078512">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.15.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="8">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="7">
        <location>
            <begin position="93"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356299.1"/>
        </source>
    </evidence>
    <sequence length="252" mass="29542" checksum="7A19674D98C5E948" modified="2006-04-04" version="1">MPATVVKKPAAKKLPAVPESKLKFSKKQISKRAAESKRRLKRAAVIALRKKENLVRAEKYQNEYIKSDQREIKLRRLAKKRNQFYVPAEAKLAFVVRIRGINKVAPKVRKVLQLFRLRQINNGVFIKLNKATINMLRIAEPYITWGYPNLKSVRELIYKRGYVKHSRQRVPITDNFVIERKLRQAHNIQCVEDLVHEIFTVGPNFKYASNFLWPFKLNTPTGGWRKKANHYVNGGDFGNREDHINRLLRKMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NU3</accession>
    <accession>A0A6I8ULL2</accession>
    <name>Q29NU3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein tomb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tomb</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357481.2">
        <property type="nucleotide sequence ID" value="XM_001357445.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NU3"/>
    <dbReference type="SMR" id="Q29NU3"/>
    <dbReference type="FunCoup" id="Q29NU3">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NU3"/>
    <dbReference type="GeneID" id="4818077"/>
    <dbReference type="KEGG" id="dpo:4818077"/>
    <dbReference type="eggNOG" id="KOG1171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1157482_0_0_1"/>
    <dbReference type="InParanoid" id="Q29NU3"/>
    <dbReference type="OMA" id="CDCYQSM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072746">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29NU3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028307">
        <property type="entry name" value="Lin-54_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446:SF34">
        <property type="entry name" value="PROTEIN LIN-54 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12446">
        <property type="entry name" value="TESMIN/TSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357481.2"/>
        </source>
    </evidence>
    <sequence length="296" mass="32773" checksum="8E3F1992945C44DE" modified="2008-10-14" version="2">MNPFPSALTTRVIAAPVKTAAGRRQPRTPKTPKESTIKGCACKRTSCIKNYCDCYQSMRICHEFCKCVDCKNSVERPLVTDNLPKNSRRQRASAVAAKVEAGVLKVGLKPAPTTPSKVIVQGKRNQPGDQPIIPLGVRIVPKPTPMKLLPDKPRGMPQEPVIIQMPQPQHQLKQLTNEPRHHVVPKFRPDQDRLMSQSMPESQAWCGSPPQSSVIVSSPSCEVTEAEKENTEEKKSKELNLFIQPVNTTLLECMLIQATEAEQLGLSDIQVGQLVLSEFFKGLQLIYDTSCGKKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PN5</accession>
    <accession>A0A6I8UL03</accession>
    <name>Q29PN5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">KiSS-1 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">LOC4817875</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357189.1">
        <property type="nucleotide sequence ID" value="XM_001357153.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035246.1">
        <property type="nucleotide sequence ID" value="XM_015179760.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PN5"/>
    <dbReference type="STRING" id="46245.Q29PN5"/>
    <dbReference type="GeneID" id="4817875"/>
    <dbReference type="KEGG" id="dpo:4817875"/>
    <dbReference type="eggNOG" id="KOG3656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="Bgee" id="FBgn0072730">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:neuropeptide receptor activity"/>
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    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Neuropeptide-GPCR"/>
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        <property type="entry name" value="CARDIOACCELERATORY PEPTIDE RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CARDIOACCELERATORY PEPTIDE RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="143"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="182"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="225"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="471"/>
            <end position="493"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="104"/>
            <end position="490"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="338"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
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    <evidence type="ECO:0000313" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357189.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015035246.1"/>
        </source>
    </evidence>
    <sequence length="562" mass="61325" checksum="A0304BEDC7061989" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-01-19" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ37</accession>
    <name>WLS_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein wntless</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">wls</name>
        <name type="ORF">GA19442</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">A segment polarity gene required for wingless (wg)-dependent patterning processes, acting in both wg-sending cells and wg-target cells. In non-neuronal cells wls directs wg secretion. The wls traffic loop encompasses the Golgi, the cell surface, an endocytic compartment and a retrograde route leading back to the Golgi, and involves clathrin-mediated endocytosis and the retromer complex (a conserved protein complex consisting of Vps35 and Vps26). In neuronal cells (the larval motorneuron NMJ), the wg signal moves across the synapse via the release of wls-containing exosome-like vesicles. Postsynaptic wls is required for the trafficking of fz2 through the fz2-interacting protein Grip.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with wg; in the Golgi. Interacts with Vps35, a component of the retromer complex; wls stability is regulated by Vps35.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Presynaptic cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Postsynaptic cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">In non-neuronal cells, wls binds to wg in the Golgi and accompanies it to the plasma membrane where the two proteins dissociate. Wg is secreted and wls is then internalized and returns to the Golgi apparatus in a retromer-dependent manner. Wls and wg colocalize in the Golgi apparatus in wg-producing cells, and reduced expression is seen in non-producing cells. Endoplasmic reticulum expression is unchanged in wg-producing versus non-producing cells. In neuronal cells, wls is localized both pre- and postsynaptically and is transferred trans-synaptically from the pre- to the postsynaptic compartment (By similarity).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the wntless family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379069">
        <property type="protein sequence ID" value="EAL29672.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001353936.2">
        <property type="nucleotide sequence ID" value="XM_001353900.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ37"/>
    <dbReference type="SMR" id="Q2LZ37"/>
    <dbReference type="FunCoup" id="Q2LZ37">
        <property type="interactions" value="640"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZ37"/>
    <dbReference type="GlyCosmos" id="Q2LZ37">
        <property type="glycosylation" value="1 site, No reported glycans"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0276995">
        <property type="protein sequence ID" value="FBpp0275433"/>
        <property type="gene ID" value="FBgn0079438"/>
    </dbReference>
    <dbReference type="GeneID" id="4813739"/>
    <dbReference type="KEGG" id="dpo:4813739"/>
    <dbReference type="CTD" id="79971"/>
    <dbReference type="eggNOG" id="ENOG502QSE2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022911_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LZ37"/>
    <dbReference type="OMA" id="GQWKWDE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079438">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
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        <property type="project" value="UniProtKB"/>
    </dbReference>
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        <property type="project" value="UniProtKB"/>
    </dbReference>
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        <property type="project" value="UniProtKB"/>
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        <property type="project" value="UniProtKB"/>
    </dbReference>
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        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
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        <property type="term" value="P:imaginal disc-derived wing margin morphogenesis"/>
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        <property type="project" value="UniProtKB"/>
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        <property type="project" value="UniProtKB"/>
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        <property type="project" value="ParkinsonsUK-UCL"/>
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        <property type="project" value="UniProtKB"/>
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        <property type="project" value="UniProtKB"/>
    </dbReference>
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        <property type="term" value="P:segment polarity determination"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
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    <dbReference type="GO" id="GO:0061355">
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047843">
        <property type="entry name" value="WLS-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053936">
        <property type="entry name" value="WLS_GOLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009551">
        <property type="entry name" value="Wntless"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449:SF2">
        <property type="entry name" value="PROTEIN WNTLESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06664">
        <property type="entry name" value="WLS-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21883">
        <property type="entry name" value="WLS_GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0966">Cell projection</keyword>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0967">Endosome</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0333">Golgi apparatus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0709">Segmentation polarity protein</keyword>
    <keyword id="KW-0770">Synapse</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="chain" id="PRO_0000390667" description="Protein wntless" evidence="4">
        <location>
            <begin position="1"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=1" evidence="4">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="35"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=2" evidence="4">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=3" evidence="4">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
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        <location>
            <begin position="312"/>
            <end position="332"/>
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            <end position="344"/>
        </location>
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        <location>
            <begin position="345"/>
            <end position="365"/>
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    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=6" evidence="4">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="408"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=7" evidence="4">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=8" evidence="4">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="504"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="538"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="539"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="4">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q5T9L3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q95ST2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EAL29672.2"/>
        </source>
    </evidence>
    <sequence length="562" mass="64040" checksum="6906680F6808FFC0" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZA5</accession>
    <accession>A0A6I8UDP2</accession>
    <name>Q2LZA5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13 14 15" type="primary">Prx4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13 14">MV-25-SWS-2005</strain>
            <tissue evidence="12 13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001353868.1">
        <property type="nucleotide sequence ID" value="XM_001353832.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043235.1">
        <property type="nucleotide sequence ID" value="XM_015187749.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043236.1">
        <property type="nucleotide sequence ID" value="XM_015187750.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240966.1">
        <property type="nucleotide sequence ID" value="XM_033385075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZA5"/>
    <dbReference type="SMR" id="Q2LZA5"/>
    <dbReference type="STRING" id="46245.Q2LZA5"/>
    <dbReference type="GeneID" id="4813859"/>
    <dbReference type="KEGG" id="dpo:4813859"/>
    <dbReference type="eggNOG" id="KOG0852">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_21_1_1"/>
    <dbReference type="OMA" id="NNFGVMR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071830">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZA5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008340">
        <property type="term" value="P:determination of adult lifespan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000003">
        <property type="entry name" value="Peroxiredoxin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF171">
        <property type="entry name" value="PEROXIREDOXIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014308672" description="thioredoxin-dependent peroxiredoxin" evidence="9">
        <location>
            <begin position="18"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="51"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001353868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015043235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015043236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033240966.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="26831" checksum="D84F1AE60E837868" modified="2006-02-21" version="1">MYKYLVVLLLAAAIVLAAKPDDGESCYSFAGGSVYPAEEPKGDHQLQYTKAVISKPAPAFEGTAVVNKEIVKLSLSQYLGKYVVLLFYPLDFTFVCPTEIIAFSDRISEFRKIKTEVIAASVDSHFTHLAWINTPRKEGGLGDVKIPLLSDLTHKISKDYGVYLESSGHALRGLFIIDQAGVLRQITMNDLPVGRSVDETIRLVQAFQYTDTHGEVCPAGWRPGADTIVPDPEEKTKYFAKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M052</accession>
    <accession>A0A6I8UD72</accession>
    <name>Q2M052_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Microfibrillar-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Mfap1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MFAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353571.2">
        <property type="nucleotide sequence ID" value="XM_001353535.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M052"/>
    <dbReference type="FunCoup" id="Q2M052">
        <property type="interactions" value="1810"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M052"/>
    <dbReference type="GeneID" id="4813679"/>
    <dbReference type="KEGG" id="dpo:4813679"/>
    <dbReference type="eggNOG" id="KOG1425">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023077_1_0_1"/>
    <dbReference type="InParanoid" id="Q2M052"/>
    <dbReference type="OMA" id="FHNERAG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070187">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M052">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="3">
        <location>
            <begin position="216"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353571.2"/>
        </source>
    </evidence>
    <sequence length="469" mass="55425" checksum="A80272BD04F7ED00" modified="2008-10-14" version="2">MSAPVAPSGIQSTAGAVPVRNEKGEISMQKVKVQRYISGKRPEYARVDSSTDESDEDDFIDTRKRMERHKAERHKMELARRGSADGKPAEDGDEAEVDDPRLRRLRARPVDMEDMERERRERHRHIHEPEMVESDSEDEEEDAEEQKNLERGTNKITLASESDTDAELSDTEMENRRTKLRARMLQQQREEEVLQKEDEKQSESSETESSEYEEESESEEDNEPRLKPLFVRKKDRATIQEKEREAQKQKQIEAEAKRAAKERRRATLRMVEDSVKKDLEKTKPESNEACLEDVCTDDDNDEVEYEAWKLRELKRMKRDREERDNTEREKLEIDRVRGLNEEERRQELRQNPKMVTNKATKGKYKFLQKYYHRGAFYLDEENDVLKRDFAQATLEDHFDKTILPKVMQVKNFGRCGRTKYTHLVDQDTTKFDCPWYAETSNNIKFHNEHAGGMRQQFDKPTGSKRKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0F7</accession>
    <accession>A0A6I8UCX1</accession>
    <name>Q2M0F7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">trypsin</fullName>
            <ecNumber evidence="11">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4813235</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001353465.1">
        <property type="nucleotide sequence ID" value="XM_001353429.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0F7"/>
    <dbReference type="SMR" id="Q2M0F7"/>
    <dbReference type="FunCoup" id="Q2M0F7">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0F7"/>
    <dbReference type="MEROPS" id="S01.A20"/>
    <dbReference type="GeneID" id="4813235"/>
    <dbReference type="KEGG" id="dpo:4813235"/>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_1_1"/>
    <dbReference type="InParanoid" id="Q2M0F7"/>
    <dbReference type="OMA" id="YKRDSCD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077346">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0F7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000034">
        <property type="entry name" value="Eupolytin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="39"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001353465.1"/>
        </source>
    </evidence>
    <sequence length="263" mass="28251" checksum="94C91538B40CB18B" modified="2006-02-21" version="1">MFVRCLVSMMLLILPILGVLGLQVVSTDSSDSMQIQPRIIGGQVSSIKQEKYLVQVTTSTEMCGGSLIQPRWVITAAHCVYRKNKDELKIYGGASNQAGPYATIRLVDFVAIRPDFSMKTLNMDVAALRLQSALVGANIETIALASQPVPPRAIVKVSGWGSTSATSSSTAQRVHSVLLPVWSRSSCLSAFKGIHGITRSMMCASKPYRRDSCDGDSGGPLVFKGQLAGIVSFGYGCASALPGVYTSVPEIREWFLNTVKLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P363</accession>
    <accession>A0A6I8VJJ7</accession>
    <name>A0A0R3P363_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26531951</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042640.1">
        <property type="nucleotide sequence ID" value="XM_015187154.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P363"/>
    <dbReference type="STRING" id="46245.A0A0R3P363"/>
    <dbReference type="GeneID" id="26531951"/>
    <dbReference type="KEGG" id="dpo:26531951"/>
    <dbReference type="InParanoid" id="A0A0R3P363"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0270938">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015042640.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="14657" checksum="7D541A008E69D76F" modified="2016-01-20" version="1">MGNQLSMNSIQDAVMDRLRSVALTTDENGAMRIRSFSEGGVGGTAGHHHHQHCSGRGGRILRESSIDGGVAVFDALLRDEHEHRLSLDAMHRVRHVRTSCTTIPEEEIPHEDLHPLHPSTAQAPSSQEVALNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBG7</accession>
    <name>A0A6I8UBG7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phospholipase A1 member A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4812147</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352869.3">
        <property type="nucleotide sequence ID" value="XM_001352833.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBG7"/>
    <dbReference type="FunCoup" id="A0A6I8UBG7">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812147"/>
    <dbReference type="InParanoid" id="A0A6I8UBG7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026093848" evidence="5">
        <location>
            <begin position="21"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="50"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352869.3"/>
        </source>
    </evidence>
    <sequence length="332" mass="36206" checksum="071A70A4F2F947F3" modified="2020-08-12" version="1">MVKTSFCLVPLFCQLWGLHAALLDSLVSQSNIYYLGPTSELPLQSVDQVATVDSVKLIVHGYLASRSHGSILPLRNAYSSQGYENVLVADWSPAASLDYPTSRFAVGKVSLALAKELQRFLLRHSIPTAAVHVVGHSLGAHIAGRIGHYFNGTLGRVTGLDPALPLFTQRSVDSLRSNAAQFVDVIHTDYPLFGDIRPRGTVDFYPNFGFAPQPGCEDVDLIAASKLLREAYSCSHNRAVMFYAESIGMPHNFPAIPCSLKAIKSRHVDSCLREARSRPSQSGTASENMVDSEEVFMGEQVSRSATSYFFLATNGAPPYGKGINSKLAKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UC72</accession>
    <name>A0A6I8UC72_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc transporter ZIP6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Zip71B</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353188.3">
        <property type="nucleotide sequence ID" value="XM_001353152.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UC72"/>
    <dbReference type="KEGG" id="dpo:4813180"/>
    <dbReference type="InParanoid" id="A0A6I8UC72"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UC72">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140410">
        <property type="term" value="F:monoatomic cation:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF31">
        <property type="entry name" value="IP18018P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026338970" evidence="8">
        <location>
            <begin position="24"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="478"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="508"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="362"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353188.3"/>
        </source>
    </evidence>
    <sequence length="575" mass="62734" checksum="E491EB2585A4846D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCJ5</accession>
    <name>A0A6I8UCJ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein Grip163</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Grip163</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353291.4">
        <property type="nucleotide sequence ID" value="XM_001353255.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UCJ5">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813265"/>
    <dbReference type="InParanoid" id="A0A6I8UCJ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCJ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF70">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="9">
        <location>
            <begin position="282"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="8">
        <location>
            <begin position="1051"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="677"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="702"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="911"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="618"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="685"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="716"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353291.4"/>
        </source>
    </evidence>
    <sequence length="1365" mass="155408" checksum="5B2688391C78B5AB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCQ5</accession>
    <name>A0A6I8UCQ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA-binding motif protein, X-linked 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353452.2">
        <property type="nucleotide sequence ID" value="XM_001353416.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCQ5"/>
    <dbReference type="KEGG" id="dpo:4813744"/>
    <dbReference type="InParanoid" id="A0A6I8UCQ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCQ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353452.2"/>
        </source>
    </evidence>
    <sequence length="183" mass="20624" checksum="F0CF5AEBC3606C94" modified="2020-08-12" version="1">MSQPNSVESKQAAPQECRDCSGKQPPATSVEVEDMGTPALEKLIRKANIAQKMLQDVLKQLEKQQVTPESRRDGKHRREHRVSKHRHSAGGKRHKGHDPATLSFSSSNSDGSVSDCELIAVSEERTSRRRHIRDDRKRDRSRSRGRSRGHSRGRSHGRSRSRSYTNPRRPTALPLALPVRISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDC8</accession>
    <name>A0A6I8UDC8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353655.3">
        <property type="nucleotide sequence ID" value="XM_001353619.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDC8"/>
    <dbReference type="FunCoup" id="A0A6I8UDC8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812912"/>
    <dbReference type="InParanoid" id="A0A6I8UDC8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026234657" evidence="1">
        <location>
            <begin position="30"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353655.3"/>
        </source>
    </evidence>
    <sequence length="224" mass="22937" checksum="0C5931164D316EBB" modified="2020-08-12" version="1">MWPPGSRQGKRYGLGVVLPLAVGLLLCSAQTQSSVTSLRSTASAAAAVAAGDTLGDAAALRATAAQILEDARPSSVVQDWSLVCKELCGAGLGVTTPGEAHSTILRPEIDAAKCGQLCGLPRDNVGDRLCSSFCQQRHRSLSGCSPCQVQEVEEQKEWKEASAVQGNATHGPSSGESLAAVAGIAAASDGTTTATPDWNELCKALCKTGDGGSLCNCDLSPFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEX0</accession>
    <name>A0A6I8UEX0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Serine protease easter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Ser7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354399.3">
        <property type="nucleotide sequence ID" value="XM_001354363.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEX0"/>
    <dbReference type="KEGG" id="dpo:4814385"/>
    <dbReference type="InParanoid" id="A0A6I8UEX0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075227">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000078">
        <property type="entry name" value="Serine protease H137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF144">
        <property type="entry name" value="GH14088P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 11 16" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026147062" evidence="13">
        <location>
            <begin position="23"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="149"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="95"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001354399.3"/>
        </source>
    </evidence>
    <sequence length="417" mass="45816" checksum="1871D6E113BF02D5" modified="2020-08-12" version="1">MLRLQGTGMLLVLLLLALQAFAQRWERTMTHFGNCHTADYGRGTCVDPGNCDFYDVDKLDASSKRQCYSRQRPDLVCCPRESNIIPALGIRINNKNNSNNNSGSTTTTERPSLRLKAGPPPNTLPPRPPTSPDQLPQYPYCGTAFASRVFGGSVAGILEFPWTTLLEYSEEGGNKSYVCGAAFIAQRWLVTAAHCTHVFFMGPGRRLTGARLGEWNKATDPDCITILNGRRECAPPHIRVSIDRILPHEQFSVKNLTNDIALLRLARPVNWLQMQHVEPVCLPPGRGPLANQLVGSAVDVSGWGRTENSESSNFKRKAMLIVQPLDECQAAFSKDGQVLNESQLCASGGIGVDSCNGDSGGPLTVEASTPQRDRFVYLAGVVSFGREECGQTEFSGVYTRISSHMDWIEQTIRANRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF20</accession>
    <name>A0A6I8UF20_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4814408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354485.3">
        <property type="nucleotide sequence ID" value="XM_001354449.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UF20"/>
    <dbReference type="KEGG" id="dpo:4814408"/>
    <dbReference type="InParanoid" id="A0A6I8UF20"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UF20">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354485.3"/>
        </source>
    </evidence>
    <sequence length="134" mass="15141" checksum="1387C1A24CA35F0B" modified="2020-08-12" version="1">MCSNLGDLFACIKPQTETETEPETVPETPDSMGNSHRRSMQVDDMEAYDLDEQLHQEMLREKEELRLSLGSLGSLSKRLVRAPRPNLPPSKHLPVPTCIRPPARGKRASFETLERYDQVFSAPSGSDRCIPDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG94</accession>
    <name>A0A6I8UG94_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TIP41-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4814680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354942.3">
        <property type="nucleotide sequence ID" value="XM_001354906.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UG94"/>
    <dbReference type="FunCoup" id="A0A6I8UG94">
        <property type="interactions" value="1954"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814680"/>
    <dbReference type="InParanoid" id="A0A6I8UG94"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UG94">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354942.3"/>
        </source>
    </evidence>
    <sequence length="272" mass="32291" checksum="9B7A8F96F9A2545C" modified="2020-08-12" version="1">MENVQCAVPRLPVDGESIRFNDWNISYEKSHILKSICKQGSTKCCDKDDTNRCELCHYQYSLDLPHLPDMVFHKNKLVLQHKDGALMEFKPMDALALVDNGKQPLEVACAQEWRHTRPEQTMEEKFKPFDWTFTSTYQGTMNEKIRSESSDMTLNKFKLMQRENIIFYTDLTLFEDELHDHGISVMSVRIRVMPSGFFILLRHFLRVDDVVMRMYDTRFHHEIENDYILKEYVHREAPCSKLQSSMAFWTNPDEMQNYLPVLSKQMHKLFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGB6</accession>
    <name>A0A6I8UGB6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein r-cup</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">r-cup</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355050.3">
        <property type="nucleotide sequence ID" value="XM_001355014.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGB6"/>
    <dbReference type="FunCoup" id="A0A6I8UGB6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814621"/>
    <dbReference type="InParanoid" id="A0A6I8UGB6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGB6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21391">
        <property type="entry name" value="AT04489P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21391:SF0">
        <property type="entry name" value="AT04489P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355050.3"/>
        </source>
    </evidence>
    <sequence length="624" mass="73406" checksum="9CF7D75B69929B67" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH57</accession>
    <name>A0A6I8UH57_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Something about silencing protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Sas10</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAS10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355407.2">
        <property type="nucleotide sequence ID" value="XM_001355371.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UH57"/>
    <dbReference type="FunCoup" id="A0A6I8UH57">
        <property type="interactions" value="1725"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815888"/>
    <dbReference type="InParanoid" id="A0A6I8UH57"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018972">
        <property type="entry name" value="Sas10_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF8">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09368">
        <property type="entry name" value="Sas10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sas10 C-terminal" evidence="6">
        <location>
            <begin position="358"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="315"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001355407.2"/>
        </source>
    </evidence>
    <sequence length="430" mass="49253" checksum="492A4990B4154E22" modified="2020-08-12" version="1">MDSEADDYELTGSEQEYDEDEREILEDLRKQRNKRKQDASAPQEILAFSDEDEEEDDNVADLMRDSDIEGADDDDAHLPNTMDWGTRRSTYYNTDFVDPDYSSYNAQEEEEAKAEEEEAKKIQLRLAKRFSEADFQLDDVPAAISDEEVDGDVAKSGIKVTADLTGLTGRERLQLLQKDSPEFLGLTQDFHLHLGEVQQLITPVLNYVRNHQVPMVPALEFASVYHNVLATYCSNVSYYLLLKARRSNVKLHPVIKRLVQLKQLREQLTSRYDTYIKPQLEALLERIQDGDAFTVLDVAQRKAKLQILNKFEQRNEKENGTATATAEDISGDSSDDEDAQLMPDQQDDDEDPEAEAVRRGITYQMAKNKGLTPHRKKELRNPRVKHRGKYRKALIRRKGAVRAVRKETQRYGGEMSGIKATVTKSVKFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UI86</accession>
    <name>A0A6I8UI86_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein kek1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kek1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355897.3">
        <property type="nucleotide sequence ID" value="XM_001355861.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UI86"/>
    <dbReference type="FunCoup" id="A0A6I8UI86">
        <property type="interactions" value="288"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816359"/>
    <dbReference type="InParanoid" id="A0A6I8UI86"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UI86">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002109">
        <property type="entry name" value="Kekkon-1, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000082">
        <property type="entry name" value="Leucine-rich repeat-containing 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF136">
        <property type="entry name" value="KEKKON 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026142314" evidence="8">
        <location>
            <begin position="18"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="332"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="537"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="706"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="772"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="866"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="712"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="723"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="783"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="816"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001355897.3"/>
        </source>
    </evidence>
    <sequence length="885" mass="94039" checksum="E358CA44508F83C5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJV2</accession>
    <name>A0A6I8UJV2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">WD repeat-containing and planar cell polarity effector protein fritz</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">frtz</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat fritz family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356549.2">
        <property type="nucleotide sequence ID" value="XM_001356513.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJV2"/>
    <dbReference type="FunCoup" id="A0A6I8UJV2">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816975"/>
    <dbReference type="InParanoid" id="A0A6I8UJV2"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJV2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097541">
        <property type="term" value="C:axonemal basal plate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024511">
        <property type="entry name" value="Frtz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13667">
        <property type="entry name" value="HOMOLOC-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13667:SF5">
        <property type="entry name" value="WD REPEAT-CONTAINING AND PLANAR CELL POLARITY EFFECTOR PROTEIN FRITZ HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11768">
        <property type="entry name" value="Frtz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="702"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="810"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="884"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="702"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="732"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="912"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001356549.2"/>
        </source>
    </evidence>
    <sequence length="933" mass="104331" checksum="53A61B4DB7596221" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKN6</accession>
    <name>A0A6I8UKN6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357033.4">
        <property type="nucleotide sequence ID" value="XM_001356997.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKN6"/>
    <dbReference type="KEGG" id="dpo:4817638"/>
    <dbReference type="InParanoid" id="A0A6I8UKN6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKN6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357033.4"/>
        </source>
    </evidence>
    <sequence length="234" mass="24537" checksum="391F4482D540105B" modified="2020-08-12" version="1">MSENSFNSKNLDDDDEDDAIAASEGKISSQVAKRIWQPTSDDTTPGVWEPPEKPLPHVEEDAPMTAEALAEALAVSETASDFIREVERSLYGDNSKDPENGTAQYFKAAENYSLFGANYMGLEEDKNKKPPPGFDQSSSQIAAGSAGVPGQSYSGGPSSSGMGGMAMSQQPQHKAANQPGPAAMPPQQQQHPRHPSPATVARVIAEAAALAGAGAGIDICLHAHGHLPKMSKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKP1</accession>
    <name>A0A6I8UKP1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein Grip71</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Grip71</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357040.2">
        <property type="nucleotide sequence ID" value="XM_001357004.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKP1"/>
    <dbReference type="FunCoup" id="A0A6I8UKP1">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817648"/>
    <dbReference type="InParanoid" id="A0A6I8UKP1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKP1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052818">
        <property type="entry name" value="NEDD1_Spindle_Assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44414">
        <property type="entry name" value="PROTEIN NEDD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44414:SF1">
        <property type="entry name" value="PROTEIN NEDD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="464"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="550"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="476"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357040.2"/>
        </source>
    </evidence>
    <sequence length="647" mass="72215" checksum="45CC04FBB30E9CCC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULT2</accession>
    <name>A0A6I8ULT2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Alanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">AlaRS</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19 20">MV-25-SWS-2005</strain>
            <tissue evidence="19 20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Monomer.</text>
    </comment>
    <comment type="domain">
        <text evidence="16">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001357433.2">
        <property type="nucleotide sequence ID" value="XM_001357397.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035521.2">
        <property type="nucleotide sequence ID" value="XM_015180035.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULT2"/>
    <dbReference type="KEGG" id="dpo:4818093"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.40:FF:000002">
        <property type="entry name" value="alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000011">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000009">
        <property type="entry name" value="Alanine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
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        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
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        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
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        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
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        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
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        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
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        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 16 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 16" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 16" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="17">
        <location>
            <begin position="4"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="604"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="608"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="723"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="727"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017959"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032577"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001357433.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015035521.2"/>
        </source>
    </evidence>
    <sequence length="966" mass="107939" checksum="97B56E4331033FF3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN93</accession>
    <name>A0A6I8UN93_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">tRNA-specific adenosine deaminase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358211.3">
        <property type="nucleotide sequence ID" value="XM_001358174.4"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GG22115"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
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        <property type="entry name" value="Cytidine_deaminase-like"/>
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        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358211.3"/>
        </source>
    </evidence>
    <sequence length="160" mass="17325" checksum="7D57FB55A01679D3" modified="2020-08-12" version="1">MAAFMEEALMEARRARDAGEVPVGCVFVHEDKVVARGANEVNVSRNATRHAEFICIDAILAYCREKNLPARQMFSEIIVVVTVEPCIMCSAALHTLGVQEIIFGCENDRFGGKTVVNVGAVVGKKIKITGGVRGDEAMALLKEFYKGDNPAAPTVAKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNJ9</accession>
    <name>A0A6I8UNJ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription elongation factor B polypeptide 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EloA</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358350.3">
        <property type="nucleotide sequence ID" value="XM_001358313.5"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8UNJ9">
        <property type="interactions" value="2247"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801227"/>
    <dbReference type="InParanoid" id="A0A6I8UNJ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051870">
        <property type="entry name" value="Elongin-A_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010684">
        <property type="entry name" value="RNA_pol_II_trans_fac_SIII_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141:SF76">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06881">
        <property type="entry name" value="Elongin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="9"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="80"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="345"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="178"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="366"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001358350.3"/>
        </source>
    </evidence>
    <sequence length="699" mass="77422" checksum="F11E7D3ADEDB4D1C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPB9</accession>
    <name>A0A6I8UPB9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">JNK1/MAPK8-associated membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4801705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358752.4">
        <property type="nucleotide sequence ID" value="XM_001358715.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPB9"/>
    <dbReference type="FunCoup" id="A0A6I8UPB9">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801705"/>
    <dbReference type="InParanoid" id="A0A6I8UPB9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008485">
        <property type="entry name" value="JAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12740">
        <property type="entry name" value="JNK1/MAPK8-ASSOCIATED MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12740:SF4">
        <property type="entry name" value="JNK1_MAPK8-ASSOCIATED MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05571">
        <property type="entry name" value="JAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358752.4"/>
        </source>
    </evidence>
    <sequence length="303" mass="34880" checksum="B9124B6A1CBACCBF" modified="2020-08-12" version="1">MYDALERCPGAYCGRSYNNDNDTWSNCGVCPRGSRVNESYACSPCRDELSTYSWLYLGFMTMLPLMMHCFFIDMDAKDRKFSRKQLILTASAFVEIALSAILATLLMEPMWELRLYACEVRKLTDWYTLFYNPSPNYEARVYCTQEAVYPLQTIVLVFYFLCLVTMFLIRPAVCKLLDVRGKSKAPIYSALYFLPLLTLVHALGCGLIYYSFPYLSVAISMVANAIHYSLKLDQTLKALVCSSVWELKNVVIISVQWLLLAYGISSLNYHYAFLCLVPFPTLFYILTVRFTDPGEFRELESRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPY8</accession>
    <name>A0A6I8UPY8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Keratin-associated protein 19-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359010.2">
        <property type="nucleotide sequence ID" value="XM_001358973.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPY8"/>
    <dbReference type="GeneID" id="4802001"/>
    <dbReference type="KEGG" id="dpo:4802001"/>
    <dbReference type="InParanoid" id="A0A6I8UPY8"/>
    <dbReference type="OMA" id="YGMRYPY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026239219" evidence="1">
        <location>
            <begin position="20"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359010.2"/>
        </source>
    </evidence>
    <sequence length="136" mass="14027" checksum="E0B41E8D42DD2635" modified="2020-08-12" version="1">MRSWLLLGLYALLIAATVGQPLEQNAPAETEGSERGERVSRQIGYPYSYGGYGGYGGYGGYGGYGGYGGYGAGIRYPYYPYGNLYGSSSLGLAGSGYYGRPYGYGNGYNIGYPLIGGFGATAGGIYGGGAPFAPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQX9</accession>
    <name>A0A6I8UQX9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">L-galactose dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359477.4">
        <property type="nucleotide sequence ID" value="XM_001359440.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQX9"/>
    <dbReference type="FunCoup" id="A0A6I8UQX9">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802584"/>
    <dbReference type="InParanoid" id="A0A6I8UQX9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010349">
        <property type="term" value="F:L-galactose dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19163">
        <property type="entry name" value="AKR_galDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000011">
        <property type="entry name" value="Aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044479">
        <property type="entry name" value="LGALDH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359477.4"/>
        </source>
    </evidence>
    <sequence length="340" mass="37942" checksum="1E7AC8E299D557AA" modified="2020-08-12" version="1">MPGELPATFVKGFHNEELVRRMEYRELGSTGLRVSKIALGGATLSSIFYNDFQREEGIKTVHEAIKSGINYIDTAPFYGNSEELLGQALKDVPREAYYIATKVARYELDPKKMFNYTAAKARESVKRSLDLLGLEQVDVLQVHDVDAAPSLDMVLNETIPVLEEYVKAGKARFIGITAYDVDVLKECAERGKGRIQVVLNYARYTLLDNTLLRHMKTFKELNVGVICAAAHSLGLLTNAGPPAIHPGSPELQALGRRAAEICKQRDVELGKLAMYYTMQLDGAATFLIGIPNRRLLQVNLDAIFDGLTSHEQEVLQYLRENVFTKSYSWGSTLSTVDKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR54</accession>
    <name>A0A6I8UR54_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Vps45</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359571.2">
        <property type="nucleotide sequence ID" value="XM_001359534.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR54"/>
    <dbReference type="FunCoup" id="A0A6I8UR54">
        <property type="interactions" value="2198"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802704"/>
    <dbReference type="InParanoid" id="A0A6I8UR54"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000003">
        <property type="entry name" value="vacuolar protein sorting-associated protein 45 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359571.2"/>
        </source>
    </evidence>
    <sequence length="574" mass="65319" checksum="E6DF8BF737AA1468" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URD2</accession>
    <name>A0A6I8URD2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Akt</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001359711.3">
        <property type="nucleotide sequence ID" value="XM_001359674.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URD2"/>
    <dbReference type="FunCoup" id="A0A6I8URD2">
        <property type="interactions" value="1086"/>
    </dbReference>
    <dbReference type="GeneID" id="4802882"/>
    <dbReference type="KEGG" id="dpo:4802882"/>
    <dbReference type="InParanoid" id="A0A6I8URD2"/>
    <dbReference type="OMA" id="VIERMFH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077857">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01241">
        <property type="entry name" value="PH_PKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05571">
        <property type="entry name" value="STKc_PKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000033">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000404">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001053">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039026">
        <property type="entry name" value="PH_PKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="185"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="443"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001359711.3"/>
        </source>
    </evidence>
    <sequence length="530" mass="59945" checksum="1EE13CEAF138EAF2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URR6</accession>
    <name>A0A6I8URR6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Antennal-specific protein OS-C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4803076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359875.4">
        <property type="nucleotide sequence ID" value="XM_001359838.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URR6"/>
    <dbReference type="KEGG" id="dpo:4803076"/>
    <dbReference type="InParanoid" id="A0A6I8URR6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026027620" description="Antennal-specific protein OS-C" evidence="2">
        <location>
            <begin position="34"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359875.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="27706" checksum="FB021BAE526F08A5" modified="2020-08-12" version="1">MSRLSSSTYSSICFLVFLHFVLLLLLTTTTTSGKPANGSQTTSKTTTTPTPTPSNGMLHVSSYVNQKRPAGPREEFDFEQNEAEATNSAHVRQLFGSLLLLPGAGNKSLEVTLETDDEPVSERNTLDELDPETASEQHEGATESADTWSRIKLAIPVLEPVKHLINAVGGGGVNDTHVRTNSHRLIGHHGVHHAPHSGGNISSPAIETEEDRETAIESTGFDVLETLGSAGTVLWGILQNLRKLFAASAGNASASSSGGGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URU6</accession>
    <name>A0A6I8URU6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Ugt302K1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001359922.2">
        <property type="nucleotide sequence ID" value="XM_001359885.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URU6"/>
    <dbReference type="FunCoup" id="A0A6I8URU6">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803128"/>
    <dbReference type="InParanoid" id="A0A6I8URU6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000144">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026371144" description="UDP-glucuronosyltransferase" evidence="5">
        <location>
            <begin position="20"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="478"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359922.2"/>
        </source>
    </evidence>
    <sequence length="520" mass="59067" checksum="E999B02A9DF71E0D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTN8</accession>
    <name>A0A6I8UTN8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">U3 small nucleolar RNA-associated protein 18 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">wcd</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat UTP18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360456.1">
        <property type="nucleotide sequence ID" value="XM_001360419.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTN8"/>
    <dbReference type="FunCoup" id="A0A6I8UTN8">
        <property type="interactions" value="2105"/>
    </dbReference>
    <dbReference type="GeneID" id="4803798"/>
    <dbReference type="KEGG" id="dpo:4803798"/>
    <dbReference type="InParanoid" id="A0A6I8UTN8"/>
    <dbReference type="OMA" id="DLNRATY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080736">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045161">
        <property type="entry name" value="Utp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359">
        <property type="entry name" value="WD-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="67"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360456.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="56124" checksum="20FB81B168388311" modified="2020-08-12" version="1">MSSDESSDGLEELKDLMAVYAKNGNRQPAARENRKKAAVPETSYVEVPMEKVIFGDKQKFLSNLAKSVGKKWNHSDDEDTADGKNDAEQSSSKRKAAWTDSDDDDIQLGDVKRPTRYTGPLTHLRKDKSYKEYLTARFQHTLNQPKWAEKQQNEDSGSSSDEELLKTVGFIDHKAKKRELRPKTLNFKRVKDLNRATYCEGFVTSVQFHPTSTAALVAGENSLATIYTVDGQKNEKLHSMRFKNYPLLCSRIAPCGTKAFFGSVRSHFYAYDLLEAKESKLYLPGDIRSMHRFEVSPCGKYIAVAGKFGAVHLLTANTNELLHSFKQEGAVRGLAWTADSKRVLCCGSSCNINILSLRQNIIEHYFIDDGCINGQCVQLAPNQRLLATGSNEGVVNVYDYESIFASKTPTPEKRFMNLRTAITDLKFNHSSELLALSSNLAPNAVKLAHFPSATVYSNFPDQNDNVSFVNTMAFSPQSSFLAFATKGKKVPLFRLKYFKGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTV4</accession>
    <name>A0A6I8UTV4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Endocuticle structural glycoprotein SgAbd-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Cpr49Af</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360859.2">
        <property type="nucleotide sequence ID" value="XM_001360822.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTV4"/>
    <dbReference type="FunCoup" id="A0A6I8UTV4">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="4804278"/>
    <dbReference type="KEGG" id="dpo:4804278"/>
    <dbReference type="InParanoid" id="A0A6I8UTV4"/>
    <dbReference type="OMA" id="AEHPYQP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081116">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF229">
        <property type="entry name" value="CUTICULAR PROTEIN 49AF, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026178997" evidence="3">
        <location>
            <begin position="17"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360859.2"/>
        </source>
    </evidence>
    <sequence length="126" mass="13961" checksum="035A8BF06493612C" modified="2020-08-12" version="1">MRTLLLLVGFVVAAWASDTSDLISQESNVEYNGKYHYHYELADGSKATQDGLLKTVNAEHDGEAVHGKYSFVSDDGNTYVVSYTADENGYHAVGDHLPTPPPTPLSVLKTLEYLKLHPYNPPEKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUI9</accession>
    <name>A0A6I8UUI9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Exocyst complex component 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Sec6</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361002.3">
        <property type="nucleotide sequence ID" value="XM_001360965.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUI9"/>
    <dbReference type="SMR" id="A0A6I8UUI9"/>
    <dbReference type="FunCoup" id="A0A6I8UUI9">
        <property type="interactions" value="1402"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804441"/>
    <dbReference type="InParanoid" id="A0A6I8UUI9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000010">
        <property type="entry name" value="exocyst complex component 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.70:FF:000001">
        <property type="entry name" value="Exocyst complex component 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361002.3"/>
        </source>
    </evidence>
    <sequence length="738" mass="86516" checksum="016BBCA53258A2FA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUS5</accession>
    <name>A0A6I8UUS5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GstE12</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361221.2">
        <property type="nucleotide sequence ID" value="XM_001361184.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUS5"/>
    <dbReference type="FunCoup" id="A0A6I8UUS5">
        <property type="interactions" value="426"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804703"/>
    <dbReference type="InParanoid" id="A0A6I8UUS5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF3">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE E11, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="90"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361221.2"/>
        </source>
    </evidence>
    <sequence length="223" mass="25024" checksum="AE43B7211B36B651" modified="2020-08-12" version="1">MSKPALYYATLSPPSRAVLLTAKAIGLDLELRPINLQKGEHLTPEFVKLNPQHTIPTLIDGDATIIDSHAICAYLVEKYGKEQQQLYPKDLVQRANVDARLHLDSGHLFARLRFLYEPILYFGSTDCSIDKIAYIQKCYEILEGFLKEQPYLCGTDLTIADFCAVATVASGNDTAPIDEFKFPKVNAWLKRLAELPYYQELNGDGAEELKAIFKAKLAENRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUX7</accession>
    <name>A0A6I8UUX7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein FAM136A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM136 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361006.2">
        <property type="nucleotide sequence ID" value="XM_001360969.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUX7"/>
    <dbReference type="FunCoup" id="A0A6I8UUX7">
        <property type="interactions" value="718"/>
    </dbReference>
    <dbReference type="GeneID" id="4804445"/>
    <dbReference type="KEGG" id="dpo:4804445"/>
    <dbReference type="InParanoid" id="A0A6I8UUX7"/>
    <dbReference type="OMA" id="NCVKDCN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078807">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008560">
        <property type="entry name" value="DUF842_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21096">
        <property type="entry name" value="PROTEIN FAM136A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21096:SF0">
        <property type="entry name" value="PROTEIN FAM136A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05811">
        <property type="entry name" value="DUF842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361006.2"/>
        </source>
    </evidence>
    <sequence length="153" mass="17753" checksum="1B30BCF3604C6061" modified="2020-08-12" version="1">MVEQQRKRLEIALSDMIEDMYRTHLRRMQSKMHRCASRCCDDERGTLESVQNCIEKCAGPLMDAQDYLQHELGQFQNRLQNCVKDCNSDVRSRLPNNPNDKDMSRSQHLFEHCTGNCVDKYINLLPGLLKSIRQTLDRGPPKRSNLTARSQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUZ2</accession>
    <name>A0A6I8UUZ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GstE1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361309.3">
        <property type="nucleotide sequence ID" value="XM_001361272.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUZ2"/>
    <dbReference type="FunCoup" id="A0A6I8UUZ2">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804808"/>
    <dbReference type="InParanoid" id="A0A6I8UUZ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF4">
        <property type="entry name" value="FI01423P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="90"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361309.3"/>
        </source>
    </evidence>
    <sequence length="222" mass="24992" checksum="3FF5FF42E8EA9DC3" modified="2020-08-12" version="1">MSKNIILYGIDMSPCVRAVQLTLKALELPYEYKEVDLQSGEHLREEFLKKNPQHTVPVLDDDGTIIWDSHAIVIYLVDKYGKSDELYPKDLAKRAAINQRLFFDASVLFPSLANVSGPFWVNGVKEVPQEKLDTIHRGLKMVETFLGSNKYLVGDSLTLADICCGPTVTSLPAAVDIDPAVYPKVTAWVERLNKLAFFEQINRAPAKNYCDFLRSQWTKLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVE9</accession>
    <name>A0A6I8UVE9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">LOC4804927</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11 12">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
            <tissue evidence="10 11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_001361410.2">
        <property type="nucleotide sequence ID" value="XM_001361373.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040296.2">
        <property type="nucleotide sequence ID" value="XM_015184810.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040297.2">
        <property type="nucleotide sequence ID" value="XM_015184811.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVE9"/>
    <dbReference type="KEGG" id="dpo:4804927"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF87">
        <property type="entry name" value="GH12965P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361410.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015040296.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015040297.2"/>
        </source>
    </evidence>
    <sequence length="440" mass="50097" checksum="311509B538ECD1B3" modified="2020-08-12" version="1">MRKYQQLVLLLISCLSVGVLLMYKTENNRLKYVLKYVNFFGRSDVAVLRRLENGTKEDLPQAALWRPMPVWQVIGDSFHAYSAYWMRKELVAGGEAHVLVVGKQGAVVDFRCSLAVGGTRSVQGKFRFQRDASEGLLNDKRTNFTSYHFFCQVSRDFGQPESVSFTDITSTRSPVKIRLHHLKRASGSTDAKAATAPSPARLPATVCVDLVGFNMTSGFGRNENALLQFFLIHQALGIEHFLVYNYDELPENIIRVLDRTNIHLYALPFNFPFLQNNGTASRVRELIQTDCLLRNVNQASFTILLQPNEVFYPNSRFAADRSQSSLQQQLRHFSADTARFELATFAVCFDELKKLLLDNIHYDPERKAPYKMLLDRLELPPAQLLASTAHVELSLSTGFVHRYVDCARVGDDGLHDWRNGVRKDFMDHINSLRNEVDLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXA1</accession>
    <name>A0A6I8UXA1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WD repeat-containing protein on Y chromosome</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6903301</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132489.2">
        <property type="nucleotide sequence ID" value="XM_002132453.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXA1"/>
    <dbReference type="GeneID" id="6903301"/>
    <dbReference type="KEGG" id="dpo:6903301"/>
    <dbReference type="InParanoid" id="A0A6I8UXA1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246913">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXA1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF6">
        <property type="entry name" value="EF-HAND CALCIUM BINDING DOMAIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="380"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="416"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="875"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="964"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1073"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="964"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="975"/>
            <end position="985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002132489.2"/>
        </source>
    </evidence>
    <sequence length="1114" mass="127196" checksum="36A79565459D2B1E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXB4</accession>
    <name>A0A6I8UXB4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132595.3">
        <property type="nucleotide sequence ID" value="XM_002132559.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXB4"/>
    <dbReference type="KEGG" id="dpo:6902859"/>
    <dbReference type="InParanoid" id="A0A6I8UXB4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132595.3"/>
        </source>
    </evidence>
    <sequence length="532" mass="61669" checksum="F06307AFB388AEE0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXE7</accession>
    <name>A0A6I8UXE7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FUN14 domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4811825</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001382220.3">
        <property type="nucleotide sequence ID" value="XM_001382183.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXE7"/>
    <dbReference type="FunCoup" id="A0A6I8UXE7">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811825"/>
    <dbReference type="InParanoid" id="A0A6I8UXE7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXE7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346">
        <property type="entry name" value="FUN14 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346:SF0">
        <property type="entry name" value="RE45833P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001382220.3"/>
        </source>
    </evidence>
    <sequence length="157" mass="17179" checksum="6A7E572F4186A7FC" modified="2020-08-12" version="1">MDDYSRIVKNTFSNISHRSAYSQMIIGVTSGWATGFATMKIGKFAAFAIGGGIILMEIAHQEGIIKVNWPRLTKGVSSIANKVESSIQGREMTWADKTERYVDDKMGKAEGLLKSNSKKVRKWYTKLIGDEDGPKVNELHIFTAAFFGGVALGIATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXI2</accession>
    <name>A0A6I8UXI2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">G2/mitotic-specific cyclin-B3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6903030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132754.2">
        <property type="nucleotide sequence ID" value="XM_002132718.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXI2"/>
    <dbReference type="KEGG" id="dpo:6903030"/>
    <dbReference type="InParanoid" id="A0A6I8UXI2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="204"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="6">
        <location>
            <begin position="297"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="301"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002132754.2"/>
        </source>
    </evidence>
    <sequence length="439" mass="49988" checksum="28C5CA842024CD55" modified="2020-08-12" version="1">MAPTKATRSSRATETSQAAMSVAIKKEKVKLSALGNNCNLSNAKIMALHQDKENCHGVLDEGKRSKATINKKTVLGEIPNGDMGQIPCRSQDQAQAQGVAIKPLRRISNKTTDSLYPSALEDVSTCGASLRPTAVAWAPVVPRRRVPRGVVDFDQKNWDDPLQVSHYSMEIFNYLKTREPAFAIGDYMPRQIHLTTDMRAVLVDWMVELQENFRLYHETLYLAVKIVDLYLCRAVIKKENLQLLGITAYFIASKYDECLPLRIWELLYICDGAYSRDELVKMELETLRLIQYDLGIPLSYSFLRRYARCAQVQRRTLTLARYILELSLMDYATIGFRDSQMASAALYMALRMRAGASHLDQQTWTPTLVHYTGFQLAEFAEIVPVLNAGLHRRPQDTIKIIRNKYSDKIFHEVAKVPLLTNQELFQNNLAPKQKSRTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXR5</accession>
    <name>A0A6I8UXR5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein c-cup</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">c-cup</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132844.2">
        <property type="nucleotide sequence ID" value="XM_002132808.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXR5"/>
    <dbReference type="FunCoup" id="A0A6I8UXR5">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903137"/>
    <dbReference type="InParanoid" id="A0A6I8UXR5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXR5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002132844.2"/>
        </source>
    </evidence>
    <sequence length="197" mass="22111" checksum="3F74FD67C3DE4C70" modified="2020-08-12" version="1">MSYSTAISMAQSLIFNLQKVGQVHLANAASRVINPIVKSTTGKMLISFAIGYYVSKSLSKILIKPADEEEPEVFREPLKLTLQQLSTFNGKNNKPTYTAFNGKIYDLTSCIMSRDDEIFRLIAGCDAGPVLQKTYKSMGISTFDHMRRWEMMMESECAVVGYLIEHYDFEGLHSYQTDEDGEDGSTVVDLSEELGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ67</accession>
    <name>A0A6I8UZ67_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6901192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133891.3">
        <property type="nucleotide sequence ID" value="XM_002133855.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ67"/>
    <dbReference type="KEGG" id="dpo:6901192"/>
    <dbReference type="InParanoid" id="A0A6I8UZ67"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ67">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133891.3"/>
        </source>
    </evidence>
    <sequence length="235" mass="25860" checksum="EC0BCD9121302327" modified="2020-08-12" version="1">MKLRSQTYREMVKILKDAGVYVEGMQTSELKQLLQTVEMSTMERIEANQENQNFESAHNEGELEVNSSHPVEVHQSTTDMLPSVLTPRFSLAPMAGDVGVNISQPGCDSPSSEPLLLSPRFSLSPIASDLGVDLSQPDCDSSSSEPLLLTPRFSLSPTAGDVGVDVSQPDCDSPPSEPLLLIVRAVVHHALEWSPSAERQTVRERRTVRERSADGNSRVLRSHSRRVQTNNKNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZG3</accession>
    <name>A0A6I8UZG3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6901545</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134220.3">
        <property type="nucleotide sequence ID" value="XM_002134184.3"/>
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        <property type="interactions" value="380"/>
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    <dbReference type="InParanoid" id="A0A6I8UZG3"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002640">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002131">
        <property type="entry name" value="Zinc finger protein 112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002286">
        <property type="entry name" value="Zinc finger protein 112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
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    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="599"/>
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        </location>
    </feature>
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    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="8">
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        <location>
            <begin position="766"/>
            <end position="793"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="75"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="146"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="209"/>
            <end position="251"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="281"/>
            <end position="379"/>
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            <end position="1033"/>
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            <begin position="1080"/>
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        </location>
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            <begin position="1161"/>
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        </location>
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            <begin position="32"/>
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        <location>
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            <end position="956"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002134220.3"/>
        </source>
    </evidence>
    <sequence length="1448" mass="157570" checksum="C70506AEE966332E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V073</accession>
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            <fullName evidence="14">WW domain binding protein VOPP1</fullName>
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        <name evidence="21 22 23" type="primary">LOC6901005</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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        <citation type="submission" date="2025-04" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21 22 23">MV-25-SWS-2005</strain>
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        <subcellularLocation>
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            <topology evidence="13">Single-pass membrane protein</topology>
        </subcellularLocation>
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            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
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        <text evidence="3">Belongs to the VOPP1/ECOP family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
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            <begin position="663"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="17">
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            <begin position="692"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006655"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00035708"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035715"/>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_002134632.3"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033237654.1"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033237655.1"/>
        </source>
    </evidence>
    <sequence length="714" mass="74946" checksum="0A4EF8259E51BE13" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1E9</accession>
    <name>A0A6I8V1E9_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Fibroin heavy chain</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">Cpr76Bd</name>
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    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135594.2">
        <property type="nucleotide sequence ID" value="XM_002135558.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V1E9">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900265"/>
    <dbReference type="InParanoid" id="A0A6I8V1E9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33289">
        <property type="entry name" value="ANCHORAGE SUBUNIT, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33289:SF2">
        <property type="entry name" value="ANCHORAGE SUBUNIT, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026154169" evidence="3">
        <location>
            <begin position="18"/>
            <end position="1341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002135594.2"/>
        </source>
    </evidence>
    <sequence length="1341" mass="132862" checksum="ED31D6EA8096A7EC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3D6</accession>
    <name>A0A6I8V3D6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137840.3">
        <property type="nucleotide sequence ID" value="XM_002137804.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3D6"/>
    <dbReference type="KEGG" id="dpo:6897734"/>
    <dbReference type="InParanoid" id="A0A6I8V3D6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137840.3"/>
        </source>
    </evidence>
    <sequence length="345" mass="36136" checksum="70FC17EB9BD8B689" modified="2020-08-12" version="1">MLHSRGIVRAHPANRAEVFDSVNPRILDMQLLLVLLLGALLSCSVAYSRSYSYDYPRRRPSPYSYNTVPQSQSNRHSRESGATAATKSDTENAKFAGASNQELDESGGDERTLLLKKKLRKLARPYLGYGGYGGYGGYGGYGGYGGYGNPCSPFGRDVKSGQKDPEEQGRFLFDVNVYKVYQGGCRGGGGLGGGFGGGLGGGLGGGLGGGLLSDPIPPPVAPYPVPVRPYAPFATWLSLFAPGVLGAATVPGVPLSDPIRPPSAGGDLQSDPAVDPSYAAPPVRRPVPNRVYYDSAGAPPVTPAQLVGGVATTVNGIIQQLTGQVQPVYQTGAYRSRSNQRSSYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3K4</accession>
    <name>A0A6I8V3K4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138096.2">
        <property type="nucleotide sequence ID" value="XM_002138060.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3K4"/>
    <dbReference type="KEGG" id="dpo:6898003"/>
    <dbReference type="InParanoid" id="A0A6I8V3K4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026314788" evidence="2">
        <location>
            <begin position="26"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138096.2"/>
        </source>
    </evidence>
    <sequence length="222" mass="24888" checksum="5137A8C898871B03" modified="2020-08-12" version="1">METQTFVLAVLVALCLCGLPQRTEAVRMKIPLIPDLPKSFDCRADSIGITYNLTALSGFWYEAARVPNVDVLECLNVSVPDAIEQDQLELDLKYISTVNGGWQYTEEQVNFPWDNDTERGVFKLQYGRIIVTYKLINTDYTTYALVCGYGNISPMPLFKLFTRQRELNQTIIAVIQAVADHQGIGNQIAWEQQSLAKCNASTMATSLGIVIGLVFLLSAYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3Q7</accession>
    <name>A0A6I8V3Q7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138093.2">
        <property type="nucleotide sequence ID" value="XM_002138057.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3Q7"/>
    <dbReference type="KEGG" id="dpo:6898000"/>
    <dbReference type="InParanoid" id="A0A6I8V3Q7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V3Q7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138093.2"/>
        </source>
    </evidence>
    <sequence length="273" mass="30898" checksum="A448A9FD335FE741" modified="2020-08-12" version="1">MATLEMALAVVSIYDGIRSCFSGKTTTSPIKPHKPSKMHSQRGRKKHRSKVHYRSHRCCGGADWSVAQRVFRGYRSETNSITSCSALSLSEFEASTDYILQQHRSSQLCEANIQPTLTPADSRFELHDCLPGLTAALDQVQHQQKRLRLLRRRTLVENAREVIGIQCASWWSRYSPDLPALSMESSLDFLSSRLSPPEREESEPGAEVMVYIRNFVNKCRKIYKAAYTVISDAYPDLSPAMDLLDINELIPAVNSALIEYLLRLEELPESNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3R8</accession>
    <name>A0A6I8V3R8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Josephin-2</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Josephin domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6898022</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002138111.1">
        <property type="nucleotide sequence ID" value="XM_002138075.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3R8"/>
    <dbReference type="GeneID" id="6898022"/>
    <dbReference type="KEGG" id="dpo:6898022"/>
    <dbReference type="InParanoid" id="A0A6I8V3R8"/>
    <dbReference type="OMA" id="QRNCEAV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245978">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V3R8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.40:FF:000003">
        <property type="entry name" value="josephin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040053">
        <property type="entry name" value="JOSD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291">
        <property type="entry name" value="JOSEPHIN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291:SF0">
        <property type="entry name" value="JOSEPHIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01233">
        <property type="entry name" value="JOSEPHIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="12">
        <location>
            <begin position="4"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002138111.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="22871" checksum="289C4DB2F24262C9" modified="2020-08-12" version="1">MPTTLKIYHEKQTRQLCALHALNNLFQENIYQKAELDNICSNLSPNAWINPHRSLLGLGNYDINVIMTALEIRHCEAFWFDKRKDPSCIDLDKIVGFILNIPLDYKIGAITLPLRKRHWIAVRRIGQHYYNLDSKLRQPELLGTESDVLHFLREQLKEKEQQRELFLVRYRSDCNESQQRWIEESHDINKTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V433</accession>
    <name>A0A6I8V433_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein moi</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">moi</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137668.3">
        <property type="nucleotide sequence ID" value="XM_002137632.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V433"/>
    <dbReference type="FunCoup" id="A0A6I8V433">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897519"/>
    <dbReference type="InParanoid" id="A0A6I8V433"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002137668.3"/>
        </source>
    </evidence>
    <sequence length="193" mass="21661" checksum="B40F5E0B76E4D3A5" modified="2020-08-12" version="1">MSQERKEGRSSPPYIDFFETLLVEELALEPNYLKLHYGKCAVIGRLTVVSDKYGLENVRVKSLPEEGSLPEGILSVLLLGLDITKASELNLSSGCYCIVRGEVVLCCVQFPNSPTLTTGGVFNLLQAMGDDEAARKNYLEELHKTHIPAIDVWFAQPVESFEELIDRRLQIKQLSLHDSHSLRLDTRMARLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9W3</accession>
    <name>A0A6I8V9W3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036797.2">
        <property type="nucleotide sequence ID" value="XM_015181311.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9W3"/>
    <dbReference type="KEGG" id="dpo:6902699"/>
    <dbReference type="InParanoid" id="A0A6I8V9W3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V9W3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026338974" evidence="2">
        <location>
            <begin position="25"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036797.2"/>
        </source>
    </evidence>
    <sequence length="234" mass="26566" checksum="5A19591AE27F61BF" modified="2020-08-12" version="1">MWNLLAVLPVILLVFQGLPVPSSSDPVWGLEFLTNLNDVVTKKIKRSMEELEDSSILEAKESYLTLKEAAELPFLQLDEKIMAYNNYEKVYGVRQNPELESDADSTTELTEKFPVKKRTIFTLLFETSVFAIFSGLGIFGSLASRVWNALLCDKKKARKLKKKIEALSCSLSKFTCKRPSHKSSSSSSSSYYGIFDIFFYSSSSPSYKKKKKGKKKKDKDCEEIQAIITDIYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAT4</accession>
    <name>A0A6I8VAT4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein Skeletor, isoforms B/C isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4801574</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037315.2">
        <property type="nucleotide sequence ID" value="XM_015181829.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAT4"/>
    <dbReference type="FunCoup" id="A0A6I8VAT4">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VAT4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019545">
        <property type="entry name" value="DM13_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052126">
        <property type="entry name" value="Spindle_Org/Thrombomodulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24036">
        <property type="entry name" value="SKELETOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24036:SF5">
        <property type="entry name" value="THROMBOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10517">
        <property type="entry name" value="DM13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00686">
        <property type="entry name" value="DM13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51549">
        <property type="entry name" value="DM13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="DM13" evidence="5">
        <location>
            <begin position="96"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="DM13" evidence="5">
        <location>
            <begin position="219"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="4">
        <location>
            <begin position="363"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015037315.2"/>
        </source>
    </evidence>
    <sequence length="766" mass="85750" checksum="D0AA1CEA11BDF34C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VD15</accession>
    <name>A0A6I8VD15_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium/hydrogen exchanger 9B1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Nha2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015038617.2">
        <property type="nucleotide sequence ID" value="XM_015183131.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VD15"/>
    <dbReference type="InParanoid" id="A0A6I8VD15"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000012">
        <property type="entry name" value="sodium/hydrogen exchanger 9B2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051843">
        <property type="entry name" value="CPA1_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102:SF21">
        <property type="entry name" value="NA[+]_H[+] HYDROGEN ANTIPORTER 2, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="586"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="10">
        <location>
            <begin position="289"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015038617.2"/>
        </source>
    </evidence>
    <sequence length="714" mass="77287" checksum="8036693C4AA4C46D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VEZ9</accession>
    <name>A0A6I8VEZ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13 14">Synaptic vesicular amine transporter isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13 14" type="primary">Vmat</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12 13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13 14">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="12 13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Vesicular transporter family.</text>
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        <property type="nucleotide sequence ID" value="XM_015184425.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039912.2">
        <property type="nucleotide sequence ID" value="XM_015184426.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234754.1">
        <property type="nucleotide sequence ID" value="XM_033378863.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8VEZ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VEZ9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005335">
        <property type="term" value="F:serotonin:sodium:chloride symporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015842">
        <property type="term" value="P:aminergic neurotransmitter loading into synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17384">
        <property type="entry name" value="MFS_SLC18A1_2_VAT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000145">
        <property type="entry name" value="Chromaffin granule amine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    </dbReference>
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        <property type="entry name" value="MFS"/>
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        <property type="entry name" value="MFS_dom"/>
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    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="MFS_Vesicular_Transporter"/>
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    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00880">
        <property type="entry name" value="2_A_01_02"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="564"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="147"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015039911.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015039912.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033234754.1"/>
        </source>
    </evidence>
    <sequence length="657" mass="69843" checksum="1F417BCC23785A51" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFW5</accession>
    <name>A0A6I8VFW5_DROPS</name>
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        <submittedName>
            <fullName evidence="9">Dystrobrevin beta isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Dyb</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040445.2">
        <property type="nucleotide sequence ID" value="XM_015184959.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFW5"/>
    <dbReference type="FunCoup" id="A0A6I8VFW5">
        <property type="interactions" value="523"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VFW5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VFW5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046716">
        <property type="term" value="P:muscle cell cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16244">
        <property type="entry name" value="EFh_DTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000014">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015154">
        <property type="entry name" value="EF-hand_dom_typ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF27">
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
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        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="7">
        <location>
            <begin position="229"/>
            <end position="285"/>
        </location>
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        <location>
            <begin position="514"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="615"/>
            <end position="636"/>
        </location>
    </feature>
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        <location>
            <begin position="433"/>
            <end position="467"/>
        </location>
    </feature>
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        <location>
            <begin position="519"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="571"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50135"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015040445.2"/>
        </source>
    </evidence>
    <sequence length="815" mass="90453" checksum="306CE244229AA387" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGJ8</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC26534339</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040850.1">
        <property type="nucleotide sequence ID" value="XM_015185364.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGJ8"/>
    <dbReference type="GeneID" id="26534339"/>
    <dbReference type="KEGG" id="dpo:26534339"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0273326">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015040850.1"/>
        </source>
    </evidence>
    <sequence length="51" mass="5704" checksum="8CDD5125E487258D" modified="2020-08-12" version="1">MFTSIRSHQHRAVGLILRALAAGSLLVLMSVIHQENDDSGSHYIHPRHAMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VH14</accession>
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        <submittedName>
            <fullName evidence="10">6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Pfrx</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
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        <property type="nucleotide sequence ID" value="XM_015185686.2"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000047">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000005">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
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    </dbReference>
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        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
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        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
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        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
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        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
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        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="8">
        <location>
            <begin position="306"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="5">
        <location>
            <position position="526"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="611"/>
        </location>
    </feature>
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        <location>
            <begin position="525"/>
            <end position="532"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="6">
        <location>
            <position position="575"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01591"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015041172.2"/>
        </source>
    </evidence>
    <sequence length="769" mass="84225" checksum="5BEC2C31B5E427D0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VH81</accession>
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        <submittedName>
            <fullName evidence="11">Plexin domain-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="11" type="primary">l(1)G0289</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_015185816.2"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031152">
        <property type="entry name" value="PLXDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055:SF12">
        <property type="entry name" value="LD40707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055">
        <property type="entry name" value="TUMOR ENDOTHELIAL MARKER 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026101084" evidence="9">
        <location>
            <begin position="28"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="586"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="505"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="513"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="550"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015041302.2"/>
        </source>
    </evidence>
    <sequence length="651" mass="70138" checksum="D9856B14E8FAD2A9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHR6</accession>
    <name>A0A6I8VHR6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Putative ATP-dependent RNA helicase DHX57</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">DEAH box protein 57</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 22 23" type="primary">LOC4814841</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21 22 23">MV-25-SWS-2005</strain>
            <tissue evidence="21 22 23">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable ATP-binding RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001354748.3">
        <property type="nucleotide sequence ID" value="XM_001354712.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041639.2">
        <property type="nucleotide sequence ID" value="XM_015186153.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041640.2">
        <property type="nucleotide sequence ID" value="XM_015186154.2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814841"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VHR6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17985">
        <property type="entry name" value="DEXHc_DHX57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23825">
        <property type="entry name" value="RWD_DHX57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000325">
        <property type="entry name" value="ATP-dependent RNA helicase DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000284">
        <property type="entry name" value="probable ATP-dependent RNA helicase YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF145">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX57-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 21 22" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="17">
        <location>
            <begin position="246"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="464"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="735"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="15">
        <location>
            <begin position="246"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001354748.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_015041639.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_015041640.2"/>
        </source>
    </evidence>
    <sequence length="1289" mass="146278" checksum="24D7C595ED69D347" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHR9</accession>
    <name>A0A6I8VHR9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cytochrome P450 4d1 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Cyp4d1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041636.2">
        <property type="nucleotide sequence ID" value="XM_015186150.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VHR9"/>
    <dbReference type="FunCoup" id="A0A6I8VHR9">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VHR9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20628">
        <property type="entry name" value="CYP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF203">
        <property type="entry name" value="CYTOCHROME P450 4D1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015041636.2"/>
        </source>
    </evidence>
    <sequence length="516" mass="58900" checksum="60C2CFC8F6966C6E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHW1</accession>
    <name>A0A6I8VHW1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytosolic carboxypeptidase Nna1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
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    </reference>
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        <property type="nucleotide sequence ID" value="XM_015186006.2"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015041492.2"/>
        </source>
    </evidence>
    <sequence length="146" mass="16214" checksum="5A6E4E13EB89DC8D" modified="2020-08-12" version="1">MADETITVTTAAAEASSFSCLLRTLSAASISRRRRHLIKPTYQPFSQPNLHPRQAATEISSECCYSSSCSDEEEDDTNNNDTHSHTHETSAERQRQQSVVVADEDSVNVNLVRANFKCNDMECSLVLGDYVNAVKKGTHTRTRTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VI92</accession>
    <name>A0A6I8VI92_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine zipper putative tumor suppressor 2 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6901613</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015041892.2">
        <property type="nucleotide sequence ID" value="XM_015186406.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VI92"/>
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        <property type="interactions" value="91"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VI92">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="LZTS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354:SF2">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0290503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2 HOMOLOG-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fez1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="324"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="549"/>
            <end position="583"/>
        </location>
    </feature>
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            <begin position="247"/>
            <end position="283"/>
        </location>
    </feature>
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            <begin position="703"/>
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        </location>
    </feature>
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        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="553"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015041892.2"/>
        </source>
    </evidence>
    <sequence length="786" mass="88124" checksum="036C35A332F547EC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLH2</accession>
    <name>A0A6I8VLH2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC4813909</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354058.3">
        <property type="nucleotide sequence ID" value="XM_001354022.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043434.2">
        <property type="nucleotide sequence ID" value="XM_015187948.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLH2"/>
    <dbReference type="KEGG" id="dpo:4813909"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354058.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015043434.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36969" checksum="63702A7A8F2BD95C" modified="2020-08-12" version="1">MATTATTDTMATMDVTTFSDYMVYLETAWEWAGCLPYLLVVYVFLTLLPKSLQRMKRFADADYEANRHKYHRCYGPELCCHVQAQEAAASKQKQKARKPVTRVYHKRQTSTTAAKEPLPPVPAPVKRSNVTKIARMFETGATRQVSRVSPVLPEIRMFGASDDSHDSTPCASRKTSIATQLSAQLQHIEQAMQLWQELEEEPSKKSSPSHSDWEPMTVPVVRRTRGTSATPSTASPLGSSPEPASPVLLRQPPSLCLQSSMEDIFQVIARSAEEPHSFYADCNSLSPVTSIDDILSERRFSRPLSAYSITDLLNEEQASCVEEATYVNDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VM18</accession>
    <name>A0A6I8VM18_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">eIF-4F 25 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eIF4E1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015044112.2">
        <property type="nucleotide sequence ID" value="XM_015188626.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VM18"/>
    <dbReference type="FunCoup" id="A0A6I8VM18">
        <property type="interactions" value="1840"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VM18"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VM18">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000010">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015044112.2"/>
        </source>
    </evidence>
    <sequence length="292" mass="32586" checksum="02CA9328902D9C74" modified="2020-08-12" version="1">MVVSETEKAICVFVSNTCVHNHPHTATNMQSDFHRMKNFANPKSMFKTSAPTTEGGNPAANTVAPGRPEPTPAVAVADPDAAPALAATLVQTKDEAKPTEAGDDVRTEHLFKHPLHNTWTLWYLENDRSKSWEDMQNEITSFDAVEDFWSLYNHIKPPSEIKLGSDYSLFKKGIRPMWEDAANKQGGRWVITLNKSSKTDLDNLWLDVLLCLIGEAFDHSDQICGAVVNIRAKSNKISIWTADGNNEEAALEIGHKLRDALRLGRQNSLQYQLHKDTMVKQGSNVKSIYTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VN53</accession>
    <name>A0A6I8VN53_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6899498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015044329.2">
        <property type="nucleotide sequence ID" value="XM_015188843.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VN53"/>
    <dbReference type="KEGG" id="dpo:6899498"/>
    <dbReference type="InParanoid" id="A0A6I8VN53"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015044329.2"/>
        </source>
    </evidence>
    <sequence length="115" mass="13146" checksum="03AB34EE4B27E664" modified="2020-08-12" version="1">MTLSGKHSGGSIPPPIQTYAFLVWAEKTCHKLCVALCPHFLVPLIEDIETLMSPNAFHQKQRASHYEVYKEEEDDDDDDDDDRPPDLTSFLPQPGVLAERYWELIVFIQNGLVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNI7</accession>
    <name>A0A6I8VNI7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117183308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232640.1">
        <property type="nucleotide sequence ID" value="XM_033376749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNI7"/>
    <dbReference type="KEGG" id="dpo:117183308"/>
    <dbReference type="InParanoid" id="A0A6I8VNI7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="2">
        <location>
            <begin position="44"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033232640.1"/>
        </source>
    </evidence>
    <sequence length="166" mass="19776" checksum="61D644C792279F49" modified="2020-08-12" version="1">MEDYMASMSPPSPSSSTLSLQSDDDMSSEYRSSLLTRSQRFNLKLLVLYSKHKCLWNFNHVDFFNTAMHKSIWADIVRQLDTKRTITWQFCRRRIMELRHYKNQYRLQQLRLDSNLPNEWYHVRDVPMSLPLDGTRGLELLPKESYKHCFYFLNSLEAPSFSPAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNL0</accession>
    <name>A0A6I8VNL0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117183310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232646.1">
        <property type="nucleotide sequence ID" value="XM_033376755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNL0"/>
    <dbReference type="KEGG" id="dpo:117183310"/>
    <dbReference type="InParanoid" id="A0A6I8VNL0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VNL0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026132433" evidence="2">
        <location>
            <begin position="23"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033232646.1"/>
        </source>
    </evidence>
    <sequence length="425" mass="46489" checksum="3E97D157359681A6" modified="2020-08-12" version="1">MSMAIVQVSLFVFLCFLAGSMAQDQARSPSTSPSTPTVPTSSSDDKLDVDFEDDDDPESGSSAKEAPMGTVPTSELTTLPTTTVSTKIPTNAVLSTTTPPKAILTKTISDETRTNQSEEAKLLALFAKNTAALNQSVILLSDILALNRNITWSLEKQKEFVPVIRYAEGLVLNATRNSFFRKAELLRSFVKAIRKIDGDASHIVDDVAYMLQLKNETKEKISSCSGKIGDIQSKMIRSSKGFDQKLNRLRQIIQQSIKPKVLNLNLTAGSINSSQVNSLAELENLPIVRKNSKSSVVQLASLEYQLAILNQTQKRSVDSLREGVQKWAPTNLSSINDQLQSLIISQKRTELDLALCDGDSSKKYHASSADEEVVYGTIVSDGFFDTRELNHQLTSNAASERYVQAGVSPKMSWYNVAQAQTGGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VP48</accession>
    <name>A0A6I8VP48_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC26533160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232802.1">
        <property type="nucleotide sequence ID" value="XM_033376911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VP48"/>
    <dbReference type="KEGG" id="dpo:26533160"/>
    <dbReference type="InParanoid" id="A0A6I8VP48"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VP48">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026516">
        <property type="entry name" value="THAP1/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021896">
        <property type="entry name" value="THAP9-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600">
        <property type="entry name" value="THAP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600:SF11">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12017">
        <property type="entry name" value="Tnp_P_element"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="8">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="245"/>
            <end position="272"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033232802.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="42052" checksum="01D752B095620BC7" modified="2020-08-12" version="1">MSNANQKDTRQSGIKCIIPSCISGYRTRSANFPVKIFRFPPNPLYFNKWVEVCGLDKNLVKRTARICERHFKAEDIGRAKLKFGAIPTLNLSDNTPLNQLHDKNSFSRNAEDNEPIENPMKSEGIKLAEVHNNISIKLEQESETETPDKDPLENNELNSNIYGENSCSGNALYNEPIEKPMKSKRINPTEVHNTISNKLEQRSEKDTPDVDPLKNNESIDVKCCENCVAKDKNEAYITRKYFELLAKKEEDVNKWKRRYASLKQSISRANANSRRKTKTNSNLSVLPIIANMSHVSAEAKTICIMLLKKSKIFSPAEKVIAQNVRFYSKRTYEYLRDVLNLQLPANITLDRWSERFGSGFTPKTGNML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSG8</accession>
    <name>A0A6I8VSG8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 10">Neurogenic protein mastermind isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10" type="primary">mam</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9 10">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="8 9 10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mastermind family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033234001.1">
        <property type="nucleotide sequence ID" value="XM_033378110.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234002.1">
        <property type="nucleotide sequence ID" value="XM_033378111.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234004.1">
        <property type="nucleotide sequence ID" value="XM_033378113.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898642"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245675">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046370">
        <property type="entry name" value="MAML_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019082">
        <property type="entry name" value="Mastermind-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491">
        <property type="entry name" value="A-TYPE INCLUSION PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34491:SF156">
        <property type="entry name" value="KINESIN MOTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09596">
        <property type="entry name" value="MamL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20802">
        <property type="entry name" value="MAML1_3_TAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01275">
        <property type="entry name" value="MamL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Neurogenic mastermind-like N-terminal" evidence="6">
        <location>
            <begin position="134"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="142"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="253"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="353"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="610"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="780"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="857"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="981"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1094"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1162"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1209"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1362"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1417"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1544"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="269"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="610"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="668"/>
            <end position="692"/>
        </location>
    </feature>
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        <location>
            <begin position="723"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="780"/>
            <end position="804"/>
        </location>
    </feature>
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        <location>
            <begin position="821"/>
            <end position="839"/>
        </location>
    </feature>
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        <location>
            <begin position="868"/>
            <end position="887"/>
        </location>
    </feature>
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        <location>
            <begin position="895"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="981"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1013"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1032"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1050"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1162"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1186"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1218"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1362"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1417"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1434"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1457"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1544"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1561"/>
            <end position="1581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033234001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033234002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033234004.1"/>
        </source>
    </evidence>
    <sequence length="1654" mass="172031" checksum="EDD854AC6777520B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSQ4</accession>
    <name>A0A6I8VSQ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13 14">Hillarin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13 14 15 16" type="primary">Hil</name>
    </gene>
    <organism evidence="11 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="14 15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15 16">MV-25-SWS-2005</strain>
            <tissue evidence="14 15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the transglutaminase-like superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360998.1">
        <property type="nucleotide sequence ID" value="XM_001360961.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039948.1">
        <property type="nucleotide sequence ID" value="XM_015184462.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234111.1">
        <property type="nucleotide sequence ID" value="XM_033378220.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234112.1">
        <property type="nucleotide sequence ID" value="XM_033378221.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234113.1">
        <property type="nucleotide sequence ID" value="XM_033378222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VSQ4"/>
    <dbReference type="GeneID" id="4804434"/>
    <dbReference type="KEGG" id="dpo:4804434"/>
    <dbReference type="OMA" id="NEFQDEW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075693">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VSQ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09443">
        <property type="entry name" value="LIM_Ltd-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000108">
        <property type="entry name" value="LIM domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056564">
        <property type="entry name" value="Ig-like_KY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053041">
        <property type="entry name" value="Transglut-like_Superfamily_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47020">
        <property type="entry name" value="HILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47020:SF1">
        <property type="entry name" value="HILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23265">
        <property type="entry name" value="Ig-like_KY"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="9"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="216"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001360998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015039948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033234111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033234112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033234113.1"/>
        </source>
    </evidence>
    <sequence length="817" mass="94470" checksum="63513CD00AAA54FC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VT42</accession>
    <name>A0A6I8VT42_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Ceramide phosphoethanolamine synthase</fullName>
        </submittedName>
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        <name evidence="4" type="primary">Cpes</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234083.1">
        <property type="nucleotide sequence ID" value="XM_033378192.1"/>
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    </dbReference>
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        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
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        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
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        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234083.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="42838" checksum="F7953D7F7C5B110F" modified="2020-08-12" version="1">MIGPSSQVSKILLTLLFLLIIFYIFMDVELYLRIHNYVIERNYHTNTSTISAPSPVAPAGTSDHSSSTKLPTAEKEPSYEDHTWISCDINPLCHVTVKAILLDHTNHYLFAPLATMFDNVIGFSRSTFITPNMISFFHVGVACLSGKLVASDSLGYRRLGVLLFQIRTFLDDLDGHVARVRKHIRGERSEIGTSGYYVDGLCDGLGCIALLLGIFFYLKNNPPRRGYSIIPMSDPKTPEPTMIPKMKATTRKVAKNVISFTGQLLLSSTAWNRYIAVYQNMLEREDVSSSQYLHCQNYVFKSNWFFCVAWMWRIVNVHSLLHLVLLSIFCDKLWDFLRTIRYSGYIILLVAICLTEMHILEAQNYIFNSTACSNISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VT65</accession>
    <name>A0A6I8VT65_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC117183673</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234257.1">
        <property type="nucleotide sequence ID" value="XM_033378366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VT65"/>
    <dbReference type="KEGG" id="dpo:117183673"/>
    <dbReference type="InParanoid" id="A0A6I8VT65"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234257.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="42959" checksum="F1F543FE1027C522" modified="2020-08-12" version="1">MKRLRDGRQARPLGANGDTTGHPSGDQRPEVPAPADEPTASRTRDWIVNPDGPSTSRQAQLRRVGEATLRQPGWMPWGQRAAAIQGWIDQQTHDPRTDAEGEVEPDGRFATAGVRSALNHDVAALNRLWLGSYGAAIDDDNATTDTDEHETILRNAPSLASTLPYEVSGHPVARGSEWDSPLWAEWDLAGQGRLGTPPAVRNEDESGDGAGPHYSDVSNAGDAGEGTGPQRDDGGSDSRSLMDLDFAPQANQHWAPANPSGSADIPPPFFAAHAGPPPQRPDEEVRDFGSEESLPLEYGEMADLLQILRLHPTVAAVQDDPEWAEAPPEWRTNAPGRRLPRDLISNITVFLRDRAYRLGVRRLEVHDGTCFRIKITRSRAVTVTLRHPRSMEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWG9</accession>
    <name>A0A6I8VWG9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235432.1">
        <property type="nucleotide sequence ID" value="XM_033379541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWG9"/>
    <dbReference type="InParanoid" id="A0A6I8VWG9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VWG9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235432.1"/>
        </source>
    </evidence>
    <sequence length="894" mass="101652" checksum="DF747ECC9A94D8D6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWN8</accession>
    <name>A0A6I8VWN8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine/arginine-rich splicing factor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6903416</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033379472.1"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:mRNA splicing, via spliceosome"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="LD23870P-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
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        <property type="entry name" value="RRM"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="438"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="526"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
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            <begin position="105"/>
            <end position="142"/>
        </location>
    </feature>
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            <begin position="195"/>
            <end position="226"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
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            <begin position="258"/>
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            <begin position="284"/>
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        <location>
            <begin position="539"/>
            <end position="561"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033235363.1"/>
        </source>
    </evidence>
    <sequence length="578" mass="62671" checksum="13EE01F6C6B6BAB9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VX84</accession>
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            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
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        </recommendedName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="11 12">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        </reaction>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="entry name" value="ZF_ZZ_2"/>
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    <keyword evidence="6" id="KW-0862">Zinc</keyword>
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        <source>
            <dbReference type="RefSeq" id="XP_001356201.2"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033235578.1"/>
        </source>
    </evidence>
    <sequence length="474" mass="52398" checksum="E6C41EFED7D056C8" modified="2020-08-12" version="1">MSRHENVVCRGCSKMGFTGRCYRCLSCPNFDICADCYDLDFTTESHLFDHPVKCVYTPADVELYFEGEYISSDPPQSYRCPYCKQWGFNEATFLEHISGMHPNASPLLVSTMITLFEQQQASRMFLENEQLAAMTAAANSRNEQMNHTEGTLVLRLEPLNPDGSYRRESSKTQTVFGDAGVDRRVVLRRPESGPSEEVPDVTTHGGVSGRRRSVLRAPGGFYFNLRTVEGENFGNSTSPGPSNESQQAPPVPSPSGHQMMNDMMRYYGTTPLPTSRASRPRTPVFSQSPLFHNGLAPGRRYFSHETGRSFFVADAFPESGLGLPHQMTDLPAFSAAPPMTATHDPHVPTPLDLGATLPLEDHDAFTLSLTEAMIKSLMASMEKPPPQEKRDTRYLCSPFLSHATRESPEQNAFLAHRAEFVSQLLASTLHEEEPAEGLGTFEVDGQNRPTQAPQMKSFPSCDLAGAGDADISPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXJ2</accession>
    <name>A0A6I8VXJ2_DROPS</name>
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        <submittedName>
            <fullName evidence="8 9">Probable elongation factor 1-delta</fullName>
        </submittedName>
    </protein>
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        <name evidence="8 9" type="primary">eEF1delta</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_033379886.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235778.1">
        <property type="nucleotide sequence ID" value="XM_033379887.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR018940">
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    </dbReference>
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        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
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        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
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        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
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        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF26">
        <property type="entry name" value="ELONGATION FACTOR 1-DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10587">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01182">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor 1 beta central acidic region eukaryote" evidence="6">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="5">
        <location>
            <begin position="161"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033235777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033235778.1"/>
        </source>
    </evidence>
    <sequence length="247" mass="28130" checksum="C57E1125D3CC6F62" modified="2020-08-12" version="1">MRVEALDMFWTDKGRYDLAEKMFHEVILKVSERNHCSPLVSEIAKAREHIQNSLEKIDVATLEDKCRLEFLDRISLLENQNIDLKSQVSKLNQAYSDVLKRLDLVENRLNCSNGNVKNNDDDVDLFASESEEETEEAARIRESRLAAYTAKKSKKAVIIAKSSLILDVKPWDDETDLQAMETVIRKIALDGLLWGASRLVPVAFGIKKLSISCVVEDDKVSIDWLTEEIEKLEDFVQSVDIAAFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VY65</accession>
    <name>A0A6I8VY65_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117184032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235923.1">
        <property type="nucleotide sequence ID" value="XM_033380032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VY65"/>
    <dbReference type="KEGG" id="dpo:117184032"/>
    <dbReference type="InParanoid" id="A0A6I8VY65"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026027253" evidence="2">
        <location>
            <begin position="17"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235923.1"/>
        </source>
    </evidence>
    <sequence length="130" mass="14464" checksum="4F6FF9B182396E25" modified="2020-08-12" version="1">MKVLIVLFSLVALALCRPQGYVFYGGQMCTMHNGRQVCGETQCFQYGGKPICRLFNYRYVDGQNDYPRQPDYPRQPDYPPSHGGSFPDRGDSGSIPDFGGPASKNTTPKPSTKPQKSSEYEVIEFSPNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYM1</accession>
    <name>A0A6I8VYM1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Aminopeptidase</fullName>
            <ecNumber evidence="23">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28 29" type="primary">LOC6902764</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28 29">MV-25-SWS-2005</strain>
            <tissue evidence="28 29">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.</text>
            <dbReference type="EC" id="3.4.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21 23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21 23">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="23"/>
    <dbReference type="RefSeq" id="XP_033236196.1">
        <property type="nucleotide sequence ID" value="XM_033380305.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236197.1">
        <property type="nucleotide sequence ID" value="XM_033380306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYM1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016285">
        <property type="term" value="F:alanyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000008">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000016">
        <property type="entry name" value="Glutamyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF253">
        <property type="entry name" value="AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="23" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 23" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7 23" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12 21 23" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="26">
        <location>
            <begin position="7"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="24">
        <location>
            <begin position="151"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="25">
        <location>
            <begin position="470"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="187"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="749"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="22">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_033236196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_033236197.1"/>
        </source>
    </evidence>
    <sequence length="818" mass="95400" checksum="80FBDC65533CB3EE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0B8</accession>
    <name>A0A6I8W0B8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Gamma-interferon-inducible lysosomal thiol reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC117184109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236204.1">
        <property type="nucleotide sequence ID" value="XM_033380313.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236205.1">
        <property type="nucleotide sequence ID" value="XM_033380314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0B8"/>
    <dbReference type="KEGG" id="dpo:117184109"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF71">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033236204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033236205.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25460" checksum="C5515A606A17BCAA" modified="2020-08-12" version="1">MVPTRKRIVIIICVVVILGCLLRILLFNSPLPTLSDQENYQRTIGAPVLVKVFYEALCPDSKYFLTKQLLPAYEAAAPIMEVLLVPYGKATTSENDGKLSFDCQHGPTECQANIYHACASEAIEDPLVRLQVVSCMIRDNRSPQEAMRKCAKQHVDLVQKCYDSKQGSELLKQNGEATHSLRPQVTFIPTITIDGYQGRQASILKNLLFEVCKAAGDTDQAKQICKNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1W3</accession>
    <name>A0A6I8W1W3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA-binding protein MEX3D isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4811686</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237322.1">
        <property type="nucleotide sequence ID" value="XM_033381431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W1W3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071003">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W1W3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22424">
        <property type="entry name" value="KH-I_MEX3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16518">
        <property type="entry name" value="RING-HC_MEX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047226">
        <property type="entry name" value="KH-I_MEX3_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047227">
        <property type="entry name" value="MEX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285:SF7">
        <property type="entry name" value="LD09246P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285">
        <property type="entry name" value="RING FINGER AND KH DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="380"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033237322.1"/>
        </source>
    </evidence>
    <sequence length="431" mass="45757" checksum="0C7A52BA2B4029F0" modified="2020-08-12" version="1">MNDSGSSGGNAGTVSRMQSGPPCLPGQVTIQVRVPYRVVGLVVGPKGATIKHIQQETQTYIVTPSREKEPIFEVTGLPDNVDTARKQIEAHIALRTGSGPGNGTGGAGTGAATGLGCESLETQEYGTQPTINSLTQILNDDLNSDILSSIYKSVISPALDYGDNTLKMVGGTVDSLNMNNVKLMPDHHIRNSYQKSELVDIDMPPIVLATASSDNHFSASEAQMLPAKVTNVYRITNKTLSFCPPTSASMNSNSGSNILTRSCSSASSTASTKSTNNSANTPPELLNIWKNISDSIDVDEGIGDSPSIWNLPANTIPTAHCSPTTSISPTDSLLGLGEHSVNRNISRHIKESSCTSQQRAPLVQFQPNSSADKLSIQRECFVCNENEVTTALVPCGHNMFCMDCANQICVSIEAACPICNSIVYHAMRILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W2V6</accession>
    <name>A0A6I8W2V6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CUGBP Elav-like family member 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">bru3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237691.1">
        <property type="nucleotide sequence ID" value="XM_033381800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W2V6"/>
    <dbReference type="FunCoup" id="A0A6I8W2V6">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W2V6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12635">
        <property type="entry name" value="RRM2_CELF3_4_5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12639">
        <property type="entry name" value="RRM3_CELF3_4_5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000007">
        <property type="entry name" value="CUGBP Elav-like family member 4 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000069">
        <property type="entry name" value="CUGBP Elav-like family member 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="71"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="374"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033237691.1"/>
        </source>
    </evidence>
    <sequence length="459" mass="48964" checksum="106211925E123F56" modified="2020-08-12" version="1">MLAFHNEHIASCAFLTYYTPESSTATAASSPTSSAAQTTTLFDDKQAISGMNRALQLKPAENESRSEHLDRKLFVGMLSKQQTEDDVRQIFHPFGSIEECTILRGPDGTSKGCAFVKFGTQQEAQSAIANLHGSQTMPGASSSLVVKYADTEKERQIRRMQQMAGHMNLLNPFVFNQFSPYGAYAQVSGMQLQLHPLLRPPSFFESLQQQQQAALMAAAATAPGSAAAYMNPMAALATQIPHGLNGTGQPPSLPSPTMPNFSMGANTPNGQPGGAAAAAAAAAADGVFTNGIPQTAFPGHPLHLTIPPQGLPNGDAATLQHAFPGLPPFPGVAFPAVYGQFPQALPPPLAAVAPTQREDFLMFPGCSISGPEGCNLFIYHLPQEFGDAELMQMFLPFGNVISSKVFIDRATNQSKCFGFVSFDNPASAQAAIQAMNGFQIGMKRLKVQLKRPKDASRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W747</accession>
    <name>A0A6I8W747_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239190.1">
        <property type="nucleotide sequence ID" value="XM_033383299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W747"/>
    <dbReference type="KEGG" id="dpo:117184685"/>
    <dbReference type="InParanoid" id="A0A6I8W747"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="531"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239190.1"/>
        </source>
    </evidence>
    <sequence length="843" mass="95955" checksum="E8B2F95DE58367B3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAW8</accession>
    <name>A0A6I8WAW8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cuticle protein 38-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239704.1">
        <property type="nucleotide sequence ID" value="XM_033383813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WAW8"/>
    <dbReference type="KEGG" id="dpo:117183194"/>
    <dbReference type="InParanoid" id="A0A6I8WAW8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054721">
        <property type="entry name" value="GEO12453p1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685:SF2">
        <property type="entry name" value="825-OAK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35685">
        <property type="entry name" value="825-OAK-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22861">
        <property type="entry name" value="GEO12453p1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026270853" evidence="1">
        <location>
            <begin position="18"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239704.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="12521" checksum="1866F5273276DF6E" modified="2020-08-12" version="1">MFKFAAVILAVLACASAKPGFLSAPLAYTAPAAIVAAPAPVVTATSSQVFARNYNGIAAAPVIAPVAAPLAAPVIAKYAAAPLATPLAYHSAPLAYSSSLASPLGYSAPLTYNTYSAFPAPAPVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDZ8</accession>
    <name>A0A6I8WDZ8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatidylinositol 4-kinase type 2</fullName>
            <ecNumber evidence="5">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC117185594</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="5"/>
    <dbReference type="RefSeq" id="XP_033241613.1">
        <property type="nucleotide sequence ID" value="XM_033385722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDZ8"/>
    <dbReference type="KEGG" id="dpo:117185594"/>
    <dbReference type="InParanoid" id="A0A6I8WDZ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026320392" description="Phosphatidylinositol 4-kinase type 2" evidence="6">
        <location>
            <begin position="24"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033241613.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="16562" checksum="45BD46E0ED994B90" modified="2020-08-12" version="1">MDQMDAQALLSLLLWTYLSEAGASLVDQKLNLNVVPKTRVVRLVAESFNYARLDRQKAKLKKRIKEHYPSAHFNRMSQPLKVNLLPCSLLILLMFSPCATSAFGRGHGPPIPKWEWPDRRYQFGQQRGGATHHEYQEYIPQIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WE90</accession>
    <name>A0A6I8WE90_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Leukocyte receptor cluster member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">LOC4801031</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241735.1">
        <property type="nucleotide sequence ID" value="XM_033385844.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241736.1">
        <property type="nucleotide sequence ID" value="XM_033385845.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241737.1">
        <property type="nucleotide sequence ID" value="XM_033385846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WE90"/>
    <dbReference type="KEGG" id="dpo:4801031"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WE90">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039875">
        <property type="entry name" value="LENG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER LRC MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093:SF0">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6 7" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="231"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033241735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033241736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033241737.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="35715" checksum="44D34BF7E354C067" modified="2020-08-12" version="1">MNILPKKRWHVRTKDNIARVRRDEAAAEAEDRKRQEKLELAESEARINFLRRQSGLPEKATVPVRSDDAAAPTPGESRGVDLFADYKTHVKTTNKDLEKEQKEEQEKYEKQIGYLTYLGQDTNEALKTRSWYEVAPKRPEREDHTATEVHLKQKLSHDPLTLINALIPPEKKAPKRAPKRKAERTPSPTPNSLAERSPTHSAHKSKKSRKDKKGSKESKKHKNKKKKSHKSVKESKELALAAERHKREKLERLRKERLRREASERQRSEALFALPKAAVPASAASTPAPTPKIVQKYNSQFNPDLAKQNMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-05-03" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9C6XPI3</accession>
    <name>A0A9C6XPI3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">NADH-ubiquinone oxidoreductase chain 4</fullName>
            <ecNumber evidence="4 17">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ND4</name>
        <name evidence="21" type="ORF">HCS66_mgp08</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="21"/>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2019-12" db="EMBL/GenBank/DDBJ databases">
            <title>Evolutionary conservation of topologically associating domains and their influences on genome synteny and structural variations in Drosophila.</title>
            <authorList>
                <person name="Liao Y."/>
                <person name="Chakraborty M."/>
                <person name="Zhang X."/>
                <person name="Emerson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">MV2-25</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="20 21" key="2">
        <citation type="submission" date="2020-03" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="NCBI Genome Project"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">MV2-25</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV2-25</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Mitochondrion membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the complex I subunit 4 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="4 17"/>
    <dbReference type="RefSeq" id="YP_009742601.1">
        <property type="nucleotide sequence ID" value="NC_046603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9C6XPI3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Mitochondrion MT"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000260">
        <property type="entry name" value="NADH4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507:SF20">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01059">
        <property type="entry name" value="Oxidored_q5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 17 21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 17" id="KW-0520">NAD</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 17" id="KW-0813">Transport</keyword>
    <keyword evidence="13 17" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase chain 4 N-terminal" evidence="19">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="18">
        <location>
            <begin position="106"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="YP_009742601.1"/>
        </source>
    </evidence>
    <sequence length="446" mass="51111" checksum="17601B68E397CDAD" modified="2023-05-03" version="1">MLKLVFFLVFLSPICFINNMYWMVQILLFFISFIFLVMNNFMNYWSEISYFLGSDMLSYGLILLSLWICSLMLLASESVNKYNNYKNLFLLNVILLLLLLVLTFSSMNLFMFYLFFESSLIPTLFLILGWGYQPERLQAGVYLLFYTLLVSLPMLIGIFYIMNEVNSMNFYLMNNYMFNYDLLYFCMLCAFLVKMPMFLVHLWLPKAHVEAPVSGSMILAGIMLKLGGYGLLRVISFLQLMNLKYSFIWISISLVGGVLVSLICLRQTDLKALIAYSSVAHMGIVLAGLLTMTYWGLCGSYTLMIAHGLCSSGLFCLANVSYERLGSRSLLINKGLLNFMPAMTLWWFLLSSANMAAPPTLNLLGEISLLNSIVSWSWLTMIMLSFLSFFSAAYTLYLYSFSQHGKLFSGVYSFSGGKIREYLLMILHWLPLNLLILSSDSCMLWL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>B5DJJ2</accession>
    <name>EIF3H_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Eukaryotic translation initiation factor 3 subunit H</fullName>
            <shortName evidence="2">eIF3h</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Eukaryotic translation initiation factor 3 subunit 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">eIF-3p40</name>
        <name evidence="2" type="synonym">eif3-S3</name>
        <name type="ORF">GA28878</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. Interacts with mxt.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-3 subunit H family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379061">
        <property type="protein sequence ID" value="EDY70464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DJJ2"/>
    <dbReference type="SMR" id="B5DJJ2"/>
    <dbReference type="FunCoup" id="B5DJJ2">
        <property type="interactions" value="2346"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DJJ2"/>
    <dbReference type="MEROPS" id="M67.971"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0289128">
        <property type="protein sequence ID" value="FBpp0287566"/>
        <property type="gene ID" value="FBgn0250237"/>
    </dbReference>
    <dbReference type="GeneID" id="6902520"/>
    <dbReference type="KEGG" id="dpo:6902520"/>
    <dbReference type="CTD" id="8667"/>
    <dbReference type="eggNOG" id="KOG1560">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044094_0_0_1"/>
    <dbReference type="InParanoid" id="B5DJJ2"/>
    <dbReference type="OMA" id="WYQSTYF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250237">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08065">
        <property type="entry name" value="MPN_eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000045">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03007">
        <property type="entry name" value="eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027524">
        <property type="entry name" value="eIF3h"/>
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    <dbReference type="InterPro" id="IPR045810">
        <property type="entry name" value="eIF3h_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19445">
        <property type="entry name" value="eIF3h_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000365190" description="Eukaryotic translation initiation factor 3 subunit H">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="MPN" evidence="3">
        <location>
            <begin position="21"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9U9Q4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01182"/>
        </source>
    </evidence>
    <sequence length="337" mass="38132" checksum="901B518B783D5371" modified="2008-10-14" version="1">MANRGGRHARTEDSDNTINYVQCDGLAVMKMVKHCHEESSNMDLAQGALLGLVVDKCLEITNCFPFPKSGDETMDEEMYQLTVMRRLRRVNVDHLHVGWYQSSDVGNSLSLALLESQYHYQTSIEESVVVVYDTQKSARGFLCLKAYRLTPQAIQMYKDGDFTPEAFRTLKVGYESLFAEIPIVIKNSPLTNIMMSELNELLPEDKGHNFLDLGTASVLENHMRSLIERVDELYQEAVRYNKYQQVVFKQDSDKNRALAKLAAENAVRTSKGEPTVAEEEVIKQFRPMPVPARLTATITSGQINTHSQHIAQFCSQSLAKLFITESLQNAKEAKEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>B5DM71</accession>
    <accession>A0A6I8UZA9</accession>
    <name>B5DM71_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitochondrial import receptor subunit TOM22 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6901260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133961.1">
        <property type="nucleotide sequence ID" value="XM_002133925.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DM71"/>
    <dbReference type="STRING" id="46245.B5DM71"/>
    <dbReference type="GeneID" id="6901260"/>
    <dbReference type="KEGG" id="dpo:6901260"/>
    <dbReference type="eggNOG" id="KOG4111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2690453_0_0_1"/>
    <dbReference type="InParanoid" id="B5DM71"/>
    <dbReference type="OMA" id="SFILWAR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248570">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DM71">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002133961.1"/>
        </source>
    </evidence>
    <sequence length="74" mass="8249" checksum="EEFE0353D6D03802" modified="2008-10-14" version="1">MDNGGYDDETDETLAERFLGLQEMFPIPVRQAVGTAANGIFNNAKKFASNPSLMFAAVSFILWARLIQKINRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>B5DRS7</accession>
    <accession>A0A6I8W9D7</accession>
    <name>B5DRS7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240003.1">
        <property type="nucleotide sequence ID" value="XM_033384112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DRS7"/>
    <dbReference type="SMR" id="B5DRS7"/>
    <dbReference type="FunCoup" id="B5DRS7">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DRS7"/>
    <dbReference type="KEGG" id="dpo:117184919"/>
    <dbReference type="KEGG" id="dpo:6900185"/>
    <dbReference type="eggNOG" id="KOG0654">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048030_0_0_1"/>
    <dbReference type="InParanoid" id="B5DRS7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249964">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd20528">
        <property type="entry name" value="CYCLIN_CCNJ-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20529">
        <property type="entry name" value="CYCLIN_CCNJ-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin C-terminal" evidence="1">
        <location>
            <begin position="172"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240003.1"/>
        </source>
    </evidence>
    <sequence length="479" mass="54190" checksum="46F35622BAE32424" modified="2016-01-20" version="2">MQVNEMAPTTIEENAFALGKRDVPDQFQLDPERLWKTHWLTVYAQDIFVNMKEAEMRRRPIVFNSMQLEERPKLLQLCIFAAQKYKLNRASVHLGIYYMDCMTDYYTISSEKLPLLALTCLHIAAQIEDNDASVPRYSELNCLIPGSLYTVFEYNVVERKVLASLNFELMRPTTASFVEFFACSFLTRNDFAHYKAMLENNPSEPNDQSPLHYESFAVMMSALSQLLLRLADCTLSITSFANTRPSLLAAACIGAVRHLSGLKCWSQYLSKLTSYLEHEVEPLVREITKYYNWQQNSSVAPNALPTYVNMGPSSADTINPNCSTTDSGIGDPMVIVKESVTVQHDNITVQVQKPAVPGSDLAGVVQEEPQEPAVFSQEELQIAAVFSQGELQKQAAFSQGELQITAVFSLAGLQEQTDLQYEEQQTQTTLELNELTLDSKEKAKKLDKEVVIKELERAFKRKRVDNDCQDEPQAKRLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>B5DU95</accession>
    <accession>A0A6I8V1I1</accession>
    <name>B5DU95_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DDB1- and CUL4-associated factor 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Dpse\GA23221</name>
        <name evidence="7" type="synonym">LOC6904059</name>
        <name evidence="5" type="ORF">Dpse_GA23221</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
                <person name="Castrezana S."/>
                <person name="Celniker S.E."/>
                <person name="Chang J.L."/>
                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
                <person name="Clifton S.W."/>
                <person name="Comeron J.M."/>
                <person name="Costello J.C."/>
                <person name="Coyne J.A."/>
                <person name="Daub J."/>
                <person name="David R.G."/>
                <person name="Delcher A.L."/>
                <person name="Delehaunty K."/>
                <person name="Do C.B."/>
                <person name="Ebling H."/>
                <person name="Edwards K."/>
                <person name="Eickbush T."/>
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                <person name="Levesque L."/>
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                <person name="Llopart A."/>
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                <person name="Marzo M."/>
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                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
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                <person name="Minx P."/>
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                <person name="Mount S.M."/>
                <person name="Mu X."/>
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                <person name="Reenan R."/>
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                <person name="Schlenke T."/>
                <person name="Schwartz R."/>
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                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
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                <person name="Spieth J."/>
                <person name="Stage D.E."/>
                <person name="Stark A."/>
                <person name="Stephan W."/>
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                <person name="Strempel S."/>
                <person name="Sturgill D."/>
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                <person name="Sutton G.G."/>
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                <person name="Venter E."/>
                <person name="Venter J.C."/>
                <person name="Vicario S."/>
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                <person name="Vilella A.J."/>
                <person name="Villasante A."/>
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                <person name="Wong G.K."/>
                <person name="Wu C.I."/>
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                <person name="Abdouelleil A."/>
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                <person name="Abebe A."/>
                <person name="Abera B."/>
                <person name="Abreu J."/>
                <person name="Acer S.C."/>
                <person name="Aftuck L."/>
                <person name="Alexander A."/>
                <person name="An P."/>
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                <person name="Anderson S."/>
                <person name="Arachi H."/>
                <person name="Azer M."/>
                <person name="Bachantsang P."/>
                <person name="Barry A."/>
                <person name="Bayul T."/>
                <person name="Berlin A."/>
                <person name="Bessette D."/>
                <person name="Bloom T."/>
                <person name="Blye J."/>
                <person name="Boguslavskiy L."/>
                <person name="Bonnet C."/>
                <person name="Boukhgalter B."/>
                <person name="Bourzgui I."/>
                <person name="Brown A."/>
                <person name="Cahill P."/>
                <person name="Channer S."/>
                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
                <person name="Costello M."/>
                <person name="D'Aco K."/>
                <person name="Daza R."/>
                <person name="De Haan G."/>
                <person name="DeGray S."/>
                <person name="DeMaso C."/>
                <person name="Dhargay N."/>
                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
                <person name="Dorjee K."/>
                <person name="Dupes A."/>
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                <person name="Honan T."/>
                <person name="Huard M.D."/>
                <person name="Hughes L."/>
                <person name="Hurhula B."/>
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                <person name="Markiewicz E."/>
                <person name="Oyono O.L."/>
                <person name="Patti C."/>
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                <person name="Priest M."/>
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                <person name="Rege F."/>
                <person name="Reyes R."/>
                <person name="Rise C."/>
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                <person name="Ryan E."/>
                <person name="Settipalli S."/>
                <person name="Shea T."/>
                <person name="Sherpa N."/>
                <person name="Shi L."/>
                <person name="Shih D."/>
                <person name="Sparrow T."/>
                <person name="Spaulding J."/>
                <person name="Stalker J."/>
                <person name="Stange-Thomann N."/>
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                <person name="Stone C."/>
                <person name="Strader C."/>
                <person name="Tesfaye S."/>
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                <person name="Topham K."/>
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                <person name="Tsamla T."/>
                <person name="Vassiliev H."/>
                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
                <person name="Weiand M."/>
                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
                <person name="Yu Q."/>
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            <dbReference type="PubMed" id="17994087"/>
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            <strain evidence="5">MV2-25</strain>
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    <reference evidence="5" key="3">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
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                <person name="English A.C."/>
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            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="5">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EDY71931.1"/>
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        <property type="nucleotide sequence ID" value="XM_002136424.3"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
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        <location>
            <begin position="52"/>
            <end position="93"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="387"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="612"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="620"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EDY71931.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002136460.1"/>
        </source>
    </evidence>
    <sequence length="634" mass="70560" checksum="A982ADB337582AB9" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>B5DUR8</accession>
    <name>FEN1_DROPS</name>
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        <recommendedName>
            <fullName evidence="1">Flap endonuclease 1</fullName>
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            <ecNumber evidence="1">3.1.-.-</ecNumber>
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        <alternativeName>
            <fullName evidence="1">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">Fen1</name>
        <name type="ORF">GA27689</name>
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    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <text evidence="1">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CH674335">
        <property type="protein sequence ID" value="EDY71637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002136633.1">
        <property type="nucleotide sequence ID" value="XM_002136597.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DUR8"/>
    <dbReference type="SMR" id="B5DUR8"/>
    <dbReference type="FunCoup" id="B5DUR8">
        <property type="interactions" value="2245"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DUR8"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0286673">
        <property type="protein sequence ID" value="FBpp0285111"/>
        <property type="gene ID" value="FBgn0249054"/>
    </dbReference>
    <dbReference type="GeneID" id="6903886"/>
    <dbReference type="KEGG" id="dpo:6903886"/>
    <dbReference type="CTD" id="2237"/>
    <dbReference type="eggNOG" id="KOG2519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032444_2_0_1"/>
    <dbReference type="InParanoid" id="B5DUR8"/>
    <dbReference type="OMA" id="MGIPWVQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249054">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000003">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000403503" description="Flap endonuclease 1">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="I-domain">
        <location>
            <begin position="122"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with PCNA" evidence="1">
        <location>
            <begin position="336"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="354"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="386" mass="43145" checksum="F26CDDAB3FE64F4A" modified="2008-10-14" version="1">MGILGLSKLIADLAPQAIRESEMKHFFGRKVAIDASMCLYQFLIAVRSEGAQLATVNGDPTSHLMGMFYRTIRLLDNGIKPVYVFDGKPPDLKSGELAKRAERREEAEKALKAATDAGDDAEIEKFNRRLVRVTKEHAREAKELLSLMGVPYVDAPCEAEAQCAALVKAGKVYATATEDMDALTFGSTKLLRYLTYSEARKMPVKEFSYDKLLEGLEVNNREFIDLCILLGCDYCESIKGIGPKRAIELINNYRDIETILDNLDTSKYTVPENWNYKVARELFIEPEVADASAIDLKWTEPDEEGLVKFLCGDRQFSEERVRNGAKKLFKSKHAQTQVRLDSFFKTLPSTPNAVNAAKRKAEEAKKSANNKKAKTSGGAARGRRPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29AU5</accession>
    <name>MTG1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mitochondrial GTPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA14342</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a role in the regulation of the mitochondrial ribosome assembly and of translational activity (By similarity). Displays mitochondrial GTPase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL27254.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29AU5"/>
    <dbReference type="SMR" id="Q29AU5"/>
    <dbReference type="FunCoup" id="Q29AU5">
        <property type="interactions" value="2090"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29AU5"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0285033">
        <property type="protein sequence ID" value="FBpp0283471"/>
        <property type="gene ID" value="FBgn0074370"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800930"/>
    <dbReference type="eggNOG" id="KOG2485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_0_2_1"/>
    <dbReference type="InParanoid" id="Q29AU5"/>
    <dbReference type="OMA" id="GVLWPKF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:mitochondrial translation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mitochondrial GTPase 1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="G_CP_dom"/>
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        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
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    <dbReference type="InterPro" id="IPR016478">
        <property type="entry name" value="GTPase_MTG1"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006230">
        <property type="entry name" value="MG442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2 3">
        <location>
            <begin position="1"/>
            <end status="unknown"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000409876" description="Mitochondrial GTPase 1" evidence="2">
        <location>
            <begin status="unknown"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="CP-type G" evidence="4">
        <location>
            <begin position="31"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="78"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="152"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O31743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9BT17"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="EAL27254.2"/>
        </source>
    </evidence>
    <sequence length="320" mass="36090" checksum="A0DE2BD1603085E6" modified="2008-10-14" version="2" precursor="true">MTAQLNPFRNAFRLPTKQRINWFPGHMNKGMRQIQQKLRNVDCIVEIHDSRIPLAGRNSQFFDTITGSGVKPHILVLNKVDLLGPKQQRSVLQQLRRQQPELKNILFTNCKDQRNHGVLDILPLATQLVGDSSRYNRAQSAEHNIMIIGVPNVGKSSIINVLRNVHLKKRSAARVGAEAGVTRAVGERIKIQEKPAVYMIDTPGILQPSVTDDEMGMKLALVGCLPDHIVGEDLIADYLLYWLNKHRRYEYVEKLSLSSGPSDNISAVLAEYAQTHGMFHRVKQYDGQVEVMTNLLAAARKFIQFFRTGQLGSINLDEHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CP5</accession>
    <accession>A0A6I8UAB5</accession>
    <name>Q29CP5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Platelet-activating factor acetylhydrolase IB subunit beta homolog</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein, isoform A</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein, isoform B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Dpse\GA21438</name>
        <name evidence="6 7" type="synonym">Paf-AHalpha</name>
        <name evidence="3" type="ORF">Dpse_GA21438</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
                <person name="Castrezana S."/>
                <person name="Celniker S.E."/>
                <person name="Chang J.L."/>
                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
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                <person name="Gotea V."/>
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                <person name="Hinrichs A.S."/>
                <person name="Holmes I."/>
                <person name="Hoskins R.A."/>
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                <person name="Hultmark D."/>
                <person name="Huntley M.A."/>
                <person name="Jaffe D.B."/>
                <person name="Jagadeeshan S."/>
                <person name="Jeck W.R."/>
                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
                <person name="Li R."/>
                <person name="Lin C.F."/>
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                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
                <person name="Machado C.A."/>
                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
                <person name="O'Grady P."/>
                <person name="Pachter L."/>
                <person name="Papaceit M."/>
                <person name="Parisi M.J."/>
                <person name="Parisi M."/>
                <person name="Parts L."/>
                <person name="Pedersen J.S."/>
                <person name="Pesole G."/>
                <person name="Phillippy A.M."/>
                <person name="Ponting C.P."/>
                <person name="Pop M."/>
                <person name="Porcelli D."/>
                <person name="Powell J.R."/>
                <person name="Prohaska S."/>
                <person name="Pruitt K."/>
                <person name="Puig M."/>
                <person name="Quesneville H."/>
                <person name="Ram K.R."/>
                <person name="Rand D."/>
                <person name="Rasmussen M.D."/>
                <person name="Reed L.K."/>
                <person name="Reenan R."/>
                <person name="Reily A."/>
                <person name="Remington K.A."/>
                <person name="Rieger T.T."/>
                <person name="Ritchie M.G."/>
                <person name="Robin C."/>
                <person name="Rogers Y.H."/>
                <person name="Rohde C."/>
                <person name="Rozas J."/>
                <person name="Rubenfield M.J."/>
                <person name="Ruiz A."/>
                <person name="Russo S."/>
                <person name="Salzberg S.L."/>
                <person name="Sanchez-Gracia A."/>
                <person name="Saranga D.J."/>
                <person name="Sato H."/>
                <person name="Schaeffer S.W."/>
                <person name="Schatz M.C."/>
                <person name="Schlenke T."/>
                <person name="Schwartz R."/>
                <person name="Segarra C."/>
                <person name="Singh R.S."/>
                <person name="Sirot L."/>
                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
                <person name="Smith T.F."/>
                <person name="Spieth J."/>
                <person name="Stage D.E."/>
                <person name="Stark A."/>
                <person name="Stephan W."/>
                <person name="Strausberg R.L."/>
                <person name="Strempel S."/>
                <person name="Sturgill D."/>
                <person name="Sutton G."/>
                <person name="Sutton G.G."/>
                <person name="Tao W."/>
                <person name="Teichmann S."/>
                <person name="Tobari Y.N."/>
                <person name="Tomimura Y."/>
                <person name="Tsolas J.M."/>
                <person name="Valente V.L."/>
                <person name="Venter E."/>
                <person name="Venter J.C."/>
                <person name="Vicario S."/>
                <person name="Vieira F.G."/>
                <person name="Vilella A.J."/>
                <person name="Villasante A."/>
                <person name="Walenz B."/>
                <person name="Wang J."/>
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                <person name="Watts T."/>
                <person name="Wilson D."/>
                <person name="Wilson R.K."/>
                <person name="Wing R.A."/>
                <person name="Wolfner M.F."/>
                <person name="Wong A."/>
                <person name="Wong G.K."/>
                <person name="Wu C.I."/>
                <person name="Wu G."/>
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                <person name="Yang H.P."/>
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                <person name="Yoshida K."/>
                <person name="Zdobnov E."/>
                <person name="Zhang P."/>
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                <person name="Zimin A.V."/>
                <person name="Baldwin J."/>
                <person name="Abdouelleil A."/>
                <person name="Abdulkadir J."/>
                <person name="Abebe A."/>
                <person name="Abera B."/>
                <person name="Abreu J."/>
                <person name="Acer S.C."/>
                <person name="Aftuck L."/>
                <person name="Alexander A."/>
                <person name="An P."/>
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                <person name="Arachi H."/>
                <person name="Azer M."/>
                <person name="Bachantsang P."/>
                <person name="Barry A."/>
                <person name="Bayul T."/>
                <person name="Berlin A."/>
                <person name="Bessette D."/>
                <person name="Bloom T."/>
                <person name="Blye J."/>
                <person name="Boguslavskiy L."/>
                <person name="Bonnet C."/>
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                <person name="Bourzgui I."/>
                <person name="Brown A."/>
                <person name="Cahill P."/>
                <person name="Channer S."/>
                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
                <person name="Costello M."/>
                <person name="D'Aco K."/>
                <person name="Daza R."/>
                <person name="De Haan G."/>
                <person name="DeGray S."/>
                <person name="DeMaso C."/>
                <person name="Dhargay N."/>
                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
                <person name="Dorjee K."/>
                <person name="Dupes A."/>
                <person name="Elong R."/>
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                <person name="Farina A."/>
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                <person name="Giannoukos G."/>
                <person name="Goode T."/>
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                <person name="Grandbois E."/>
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                <person name="Gyaltsen K."/>
                <person name="Hafez N."/>
                <person name="Hagos B."/>
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                <person name="Henson C."/>
                <person name="Hollinger A."/>
                <person name="Honan T."/>
                <person name="Huard M.D."/>
                <person name="Hughes L."/>
                <person name="Hurhula B."/>
                <person name="Husby M.E."/>
                <person name="Kamat A."/>
                <person name="Kanga B."/>
                <person name="Kashin S."/>
                <person name="Khazanovich D."/>
                <person name="Kisner P."/>
                <person name="Lance K."/>
                <person name="Lara M."/>
                <person name="Lee W."/>
                <person name="Lennon N."/>
                <person name="Letendre F."/>
                <person name="LeVine R."/>
                <person name="Lipovsky A."/>
                <person name="Liu X."/>
                <person name="Liu J."/>
                <person name="Liu S."/>
                <person name="Lokyitsang T."/>
                <person name="Lokyitsang Y."/>
                <person name="Lubonja R."/>
                <person name="Lui A."/>
                <person name="MacDonald P."/>
                <person name="Magnisalis V."/>
                <person name="Maru K."/>
                <person name="Matthews C."/>
                <person name="McCusker W."/>
                <person name="McDonough S."/>
                <person name="Mehta T."/>
                <person name="Meldrim J."/>
                <person name="Meneus L."/>
                <person name="Mihai O."/>
                <person name="Mihalev A."/>
                <person name="Mihova T."/>
                <person name="Mittelman R."/>
                <person name="Mlenga V."/>
                <person name="Montmayeur A."/>
                <person name="Mulrain L."/>
                <person name="Navidi A."/>
                <person name="Naylor J."/>
                <person name="Negash T."/>
                <person name="Nguyen T."/>
                <person name="Nguyen N."/>
                <person name="Nicol R."/>
                <person name="Norbu C."/>
                <person name="Norbu N."/>
                <person name="Novod N."/>
                <person name="O'Neill B."/>
                <person name="Osman S."/>
                <person name="Markiewicz E."/>
                <person name="Oyono O.L."/>
                <person name="Patti C."/>
                <person name="Phunkhang P."/>
                <person name="Pierre F."/>
                <person name="Priest M."/>
                <person name="Raghuraman S."/>
                <person name="Rege F."/>
                <person name="Reyes R."/>
                <person name="Rise C."/>
                <person name="Rogov P."/>
                <person name="Ross K."/>
                <person name="Ryan E."/>
                <person name="Settipalli S."/>
                <person name="Shea T."/>
                <person name="Sherpa N."/>
                <person name="Shi L."/>
                <person name="Shih D."/>
                <person name="Sparrow T."/>
                <person name="Spaulding J."/>
                <person name="Stalker J."/>
                <person name="Stange-Thomann N."/>
                <person name="Stavropoulos S."/>
                <person name="Stone C."/>
                <person name="Strader C."/>
                <person name="Tesfaye S."/>
                <person name="Thomson T."/>
                <person name="Thoulutsang Y."/>
                <person name="Thoulutsang D."/>
                <person name="Topham K."/>
                <person name="Topping I."/>
                <person name="Tsamla T."/>
                <person name="Vassiliev H."/>
                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
                <person name="Weiand M."/>
                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
                <person name="Yu Q."/>
                <person name="Zembek L."/>
                <person name="Zhong D."/>
                <person name="Zimmer A."/>
                <person name="Zwirko Z."/>
                <person name="Jaffe D.B."/>
                <person name="Alvarez P."/>
                <person name="Brockman W."/>
                <person name="Butler J."/>
                <person name="Chin C."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Kleber M."/>
                <person name="Mauceli E."/>
                <person name="MacCallum I."/>
            </authorList>
            <dbReference type="PubMed" id="17994087"/>
            <dbReference type="DOI" id="10.1038/nature06341"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
            <authorList>
                <person name="English A.C."/>
                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CH475440">
        <property type="protein sequence ID" value="EAL29371.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CH475440">
        <property type="protein sequence ID" value="KRT05251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001352315.2">
        <property type="nucleotide sequence ID" value="XM_001352279.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044334.1">
        <property type="nucleotide sequence ID" value="XM_015188848.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29CP5"/>
    <dbReference type="SMR" id="Q29CP5"/>
    <dbReference type="STRING" id="46245.Q29CP5"/>
    <dbReference type="GeneID" id="4811743"/>
    <dbReference type="KEGG" id="dpo:4811743"/>
    <dbReference type="eggNOG" id="KOG1388">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051989_2_0_1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081425">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd01820">
        <property type="entry name" value="PAF_acetylesterase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11852">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11852:SF0">
        <property type="entry name" value="PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE IB SUBUNIT BETA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="47"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EAL29371.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KRT05251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352315.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015044334.1"/>
        </source>
    </evidence>
    <sequence length="233" mass="26380" checksum="EADC193968F298B6" modified="2016-01-20" version="2">MQNIPNTNMNPCVLPTPVPDDAGDKRWHSIHRRFISDCREKDPDVIFLGDCIFETLQDSETWNQYFAPLHCLNFSIRDDCTEHVLWRIENGALDNVNPKIVVLHVGTNNVNNSAAEVAEGILANVAKICEKLNGAYVILPSLLPRGQQPNKLREKNAKINELVKEMTKGMDRVQTVAIDKGLVQCDGTISHHDMFDYKNLTNTGAKKIFEPLYELLSQILNENELERDLTPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2025-10-08" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CT0</accession>
    <accession>A0A6I8UAE9</accession>
    <name>Q29CT0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Apolipophorins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">apolpp</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352351.1">
        <property type="nucleotide sequence ID" value="XM_001352315.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044443.1">
        <property type="nucleotide sequence ID" value="XM_015188957.2"/>
    </dbReference>
    <dbReference type="IntAct" id="Q29CT0">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29CT0"/>
    <dbReference type="GeneID" id="4811782"/>
    <dbReference type="KEGG" id="dpo:4811782"/>
    <dbReference type="eggNOG" id="KOG4338">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_225812_0_0_1"/>
    <dbReference type="OMA" id="MTWTILE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070792">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.80.10">
        <property type="entry name" value="Lipovitellin-phosvitin complex, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.50.20">
        <property type="entry name" value="Lipovitellin. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009454">
        <property type="entry name" value="Lipid_transpt_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015255">
        <property type="entry name" value="Vitellinogen_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015817">
        <property type="entry name" value="Vitellinogen_open_b-sht_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050733">
        <property type="entry name" value="Vitellogenin/Apolipophorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345:SF36">
        <property type="entry name" value="APOLIPOPHORINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06448">
        <property type="entry name" value="DUF1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09172">
        <property type="entry name" value="Vit_open_b-sht"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01169">
        <property type="entry name" value="DUF1943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014308545" evidence="8">
        <location>
            <begin position="24"/>
            <end position="3355"/>
        </location>
    </feature>
    <feature type="domain" description="Vitellogenin" evidence="9">
        <location>
            <begin position="43"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="10">
        <location>
            <begin position="2790"/>
            <end position="2956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001352351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015044443.1"/>
        </source>
    </evidence>
    <sequence length="3355" mass="371734" checksum="D491DCD89050A7B2" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DA7</accession>
    <accession>A0A6I8UBZ8</accession>
    <name>Q29DA7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 26C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4812243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex. Component of the retriever complex, which is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1). The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes. In the endosomes, drives the retriever and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex. Component of the heterotrimeric retriever complex consisting of VPS26C, VPS29 and VPS35L; within the complex interacts with VPS35L. Interacts with SNX17 (via C-terminus); the interaction is direct and associates SNX17 with the retriever complex. Interacts with SNX31; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS26 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353006.3">
        <property type="nucleotide sequence ID" value="XM_001352970.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DA7"/>
    <dbReference type="SMR" id="Q29DA7"/>
    <dbReference type="FunCoup" id="Q29DA7">
        <property type="interactions" value="1541"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DA7"/>
    <dbReference type="GeneID" id="4812243"/>
    <dbReference type="KEGG" id="dpo:4812243"/>
    <dbReference type="eggNOG" id="KOG2717">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056829_0_0_1"/>
    <dbReference type="InParanoid" id="Q29DA7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077947">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DA7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000009">
        <property type="entry name" value="Down syndrome critical region protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000033">
        <property type="entry name" value="Down syndrome critical region protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001353006.3"/>
        </source>
    </evidence>
    <sequence length="322" mass="36408" checksum="747D58EB13B99928" modified="2016-01-20" version="3">MSFNFEIKLKRENKVYYENDMLLGCVQFQCGAETKHEGIILYLEGVVNLQLSSKTVGLFDAFYNSVKPINLLQNNLELSAPGKLSAGRSEFHFELPLVCRKEPRILYETYHGVFINVNYQLQCTVKRNFLGKSMTKIQQFCVQYKPTGLSENSLRAVPFSLSPDSLQKNASAKERLSMPRFLITGRLDRSESCVTMPITGSIMVQHTEAAIKSIDMQLVRVETCGCDEGYSKDATEIQTIQIADGNVLPKLELPIYMVLPRLFTCPTLLTKNFKIEFELNLVVVFKEDYTVSENFKIVFKRSAGPLPSKMTTAGRCPIEYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DH8</accession>
    <accession>A0A6I8VLV2</accession>
    <name>Q29DH8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BET1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Golgi vesicular membrane-trafficking protein p18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23 24 25 26 27" type="primary">Bet1</name>
    </gene>
    <organism evidence="22 26">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24 25" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23 24 25">MV-25-SWS-2005</strain>
            <tissue evidence="23 24 25">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE involved in the docking process of ER-derived vesicles with the cis-Golgi membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with SNARE complex members GOSR2, SEC22B and STX5. Interacts with LMAN1/ERGIC53. Interacts with STX17.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the BET1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352935.2">
        <property type="nucleotide sequence ID" value="XM_001352899.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044011.1">
        <property type="nucleotide sequence ID" value="XM_015188525.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044012.1">
        <property type="nucleotide sequence ID" value="XM_015188526.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044013.1">
        <property type="nucleotide sequence ID" value="XM_015188527.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241393.1">
        <property type="nucleotide sequence ID" value="XM_033385502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DH8"/>
    <dbReference type="STRING" id="46245.Q29DH8"/>
    <dbReference type="GeneID" id="4811984"/>
    <dbReference type="KEGG" id="dpo:4811984"/>
    <dbReference type="eggNOG" id="KOG3385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086133_2_1_1"/>
    <dbReference type="OMA" id="GHRNYMC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072798">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DH8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000026">
        <property type="entry name" value="BET1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="21">
        <location>
            <begin position="25"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_001352935.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_015044011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_015044012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_015044013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_033241393.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="13363" checksum="06A80F33D0653344" modified="2008-10-14" version="2">MRRTNNYPYQPLNQQPSGPSPAGHDALEAENERAAEELKQKIGALKSLTIDIGNEVRYQDKLLRGIDDDMDRTSGFLGNTMTRVVRLAKQGGGAKQMCYMFLFVLVVFLLLWLTLKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FC8</accession>
    <accession>A0A6I8UEL8</accession>
    <name>Q29FC8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein Asterix</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4814166</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Asterix family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354274.1">
        <property type="nucleotide sequence ID" value="XM_001354238.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FC8"/>
    <dbReference type="FunCoup" id="Q29FC8">
        <property type="interactions" value="969"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FC8"/>
    <dbReference type="GeneID" id="4814166"/>
    <dbReference type="KEGG" id="dpo:4814166"/>
    <dbReference type="eggNOG" id="KOG3462">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128526_1_1_1"/>
    <dbReference type="InParanoid" id="Q29FC8"/>
    <dbReference type="OMA" id="MFGLMMK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070542">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FC8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005351">
        <property type="entry name" value="ASTER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13193">
        <property type="entry name" value="CGI-140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13193:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT ASTERIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03669">
        <property type="entry name" value="ASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354274.1"/>
        </source>
    </evidence>
    <sequence length="108" mass="11980" checksum="B7868CD2DA4F927E" modified="2006-04-04" version="1">MSNTVDPRRKEKINRYKAPKNQGQGGGANEDMMPDYMNILGMIFSMCGLMMKLKWCAWFALYCSCISFASSKASDDAKQVLSSFMLSVSAVVMSYLQNPAAMTPPWAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GV7</accession>
    <accession>A0A6I8UG46</accession>
    <name>Q29GV7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ras-related protein Rab-35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Rab35</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354946.1">
        <property type="nucleotide sequence ID" value="XM_001354910.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GV7"/>
    <dbReference type="FunCoup" id="Q29GV7">
        <property type="interactions" value="1385"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GV7"/>
    <dbReference type="GeneID" id="4814496"/>
    <dbReference type="KEGG" id="dpo:4814496"/>
    <dbReference type="eggNOG" id="KOG0079">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_1_1"/>
    <dbReference type="InParanoid" id="Q29GV7"/>
    <dbReference type="OMA" id="HEIDTNC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081870">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29GV7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002481">
        <property type="entry name" value="MIP14774p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001354946.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="22814" checksum="B959760A54B033FF" modified="2006-04-04" version="1">MARGFDHLFKLLIIGDSGVGKSSLLIRFSDDTFSGSYITTIGVDFKIRTVDIDGLRVKLQIWDTAGQERFRTITSTYYRGTHGVIIVYDVTNGDSFANVRRWLEEIQNNCDVVNKVLVGNKNDDPDCKVVITEDAQRFAKQMDIELYETSAKDNINVDNMFLSITRQVLNHKLRTTTNEQQKDTIHLKNNPGTKRRKCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MS6</accession>
    <accession>A0A6I8VVQ5</accession>
    <name>Q29MS6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7 10">2.1.1.216</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Trm1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7 10"/>
    <dbReference type="RefSeq" id="XP_033235170.1">
        <property type="nucleotide sequence ID" value="XM_033379279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MS6"/>
    <dbReference type="FunCoup" id="Q29MS6">
        <property type="interactions" value="2509"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MS6"/>
    <dbReference type="KEGG" id="dpo:4817434"/>
    <dbReference type="eggNOG" id="KOG1253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010862_4_1_1"/>
    <dbReference type="InParanoid" id="Q29MS6"/>
    <dbReference type="OMA" id="MKCCHEM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079553">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="501"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="539"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="571"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033235170.1"/>
        </source>
    </evidence>
    <sequence length="586" mass="65334" checksum="D923F8E8BEDF0A57" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NT6</accession>
    <accession>A0A6I8ULX3</accession>
    <name>Q29NT6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transforming growth factor beta-1-induced transcript 1 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4818072</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357488.1">
        <property type="nucleotide sequence ID" value="XM_001357452.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NT6"/>
    <dbReference type="SMR" id="Q29NT6"/>
    <dbReference type="FunCoup" id="Q29NT6">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NT6"/>
    <dbReference type="GeneID" id="4818072"/>
    <dbReference type="KEGG" id="dpo:4818072"/>
    <dbReference type="eggNOG" id="KOG1703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001357_4_1_1"/>
    <dbReference type="InParanoid" id="Q29NT6"/>
    <dbReference type="OMA" id="DYEDMFA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076611">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000008">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000005">
        <property type="entry name" value="Testin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205:SF16">
        <property type="entry name" value="GH01042P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="64"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="125"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357488.1"/>
        </source>
    </evidence>
    <sequence length="177" mass="20000" checksum="80D89B8DE56CDF7E" modified="2006-04-04" version="1">MTENIVCHKCNQAITKRMVTALGKTWHPEHFLCRHCEQPIDDAAFNIQDGEPVCTKCFIERYTHTCAGCKKPILERTIVAMGENWHEECFCCGGACKKPLSNQPFFERGGKAYCKQDYENLFADKCAKCEQPITTSAIVAMNVKWHHDCFRCNRCSNPITTPNFTVEGGQPICPACD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q29P87</accession>
    <accession>A0A6I8ULL1</accession>
    <name>Q29P87_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DET1- and DDB1-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4817949</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357337.1">
        <property type="nucleotide sequence ID" value="XM_001357301.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29P87"/>
    <dbReference type="FunCoup" id="Q29P87">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29P87"/>
    <dbReference type="GeneID" id="4817949"/>
    <dbReference type="KEGG" id="dpo:4817949"/>
    <dbReference type="eggNOG" id="KOG4816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144562_1_0_1"/>
    <dbReference type="InParanoid" id="Q29P87"/>
    <dbReference type="OMA" id="RYLHQHW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076541">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29P87">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033575">
        <property type="entry name" value="DDA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018276">
        <property type="entry name" value="DDA1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879:SF2">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10172">
        <property type="entry name" value="DDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DET1- and DDB1-associated protein 1" evidence="5">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="67"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001357337.1"/>
        </source>
    </evidence>
    <sequence length="106" mass="12089" checksum="6A2D0B1179467260" modified="2006-04-04" version="1">MSVSDFIKGLPIHDSNNFTHLSNEHGIRTSQKRASVYLPTEDEASEQLIVMDKRCVLLRYLTQQWDKKTLQRKREHGNEGNANSSTPIGNGSNSKKRPRIDPNELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PI0</accession>
    <accession>A0A6I8UL47</accession>
    <name>Q29PI0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Gdi</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates the GDP/GTP exchange reaction of most RAB proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357244.1">
        <property type="nucleotide sequence ID" value="XM_001357208.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PI0"/>
    <dbReference type="SMR" id="Q29PI0"/>
    <dbReference type="FunCoup" id="Q29PI0">
        <property type="interactions" value="1922"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29PI0"/>
    <dbReference type="GeneID" id="4818091"/>
    <dbReference type="KEGG" id="dpo:4818091"/>
    <dbReference type="eggNOG" id="KOG1439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021695_0_0_1"/>
    <dbReference type="InParanoid" id="Q29PI0"/>
    <dbReference type="OMA" id="FETKAKM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078179">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29PI0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000001">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000158">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000232">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF8">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357244.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="49900" checksum="733C6AB63D0ACD1A" modified="2006-04-04" version="1">MNEEYDAIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASITPLEELFQRYGLEPPGERFGRGRDWNVDLIPKFLMANGQLVKLLIHTGVTRYLEFKSIEGSYVYKGGKIAKVPVDQKEALASDLMGMFEKRRFRNFLIYVQDFREEDPKTWKDFDPTKANMQGLYDKFGLDKNTQDFTGHALALFRDDEYLNEPAVNTIRRIKLYSDSLARYGKSPYLYPMYGLGELPQGFARLSAIYGGTYMLDKPIDEIVLGEGGKVVGVRSGEEVAKCKQVYCDPSYVPEKVRKRGKVIRCICILDHPVASTKDGLSTQIIIPQKQVGRKSDIYVSLVSSTHQVAAKGWFVGMVSTTVETENPEVEIKPGLDLLEPIAQKFVTISDYLEPIDDGAQSQIFISESYDATTHFETTCLDVLNIFKRGTGETFDFSKIKHELGDEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PM6</accession>
    <accession>A0A6I8ULA9</accession>
    <name>Q29PM6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal RNA-processing protein 7 homolog A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4818010</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357198.2">
        <property type="nucleotide sequence ID" value="XM_001357162.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PM6"/>
    <dbReference type="FunCoup" id="Q29PM6">
        <property type="interactions" value="1633"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29PM6"/>
    <dbReference type="GeneID" id="4818010"/>
    <dbReference type="KEGG" id="dpo:4818010"/>
    <dbReference type="eggNOG" id="KOG4008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036234_2_0_1"/>
    <dbReference type="InParanoid" id="Q29PM6"/>
    <dbReference type="OMA" id="GIHKWIA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081533">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12294">
        <property type="entry name" value="RRM_Rrp7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12951">
        <property type="entry name" value="RRP7_Rrp7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040447">
        <property type="entry name" value="RRM_Rrp7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040446">
        <property type="entry name" value="RRP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024326">
        <property type="entry name" value="RRP7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034890">
        <property type="entry name" value="Rrp7A_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13191:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 7 HOMOLOG A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17799">
        <property type="entry name" value="RRM_Rrp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12923">
        <property type="entry name" value="RRP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rrp7 RRM-like N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 7 C-terminal" evidence="2">
        <location>
            <begin position="151"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357198.2"/>
        </source>
    </evidence>
    <sequence length="266" mass="30897" checksum="19B5206A5A341D6F" modified="2008-10-14" version="2">MEEIEGYTVVPLRMSSDALHCHSVYMREHFIRLMDPNKPKGRTLFLLNIPPYVTEESLKGFFKRAGNVESVQFAAKPGKDETIKWYEGTNEPFSNVRPPFIFKVAYVVFQKSSSIGKALAVPSIDLFHSSGESTIKTGMELWHEEYENHYILDAEKTKAQITEYMADYDRRERAAALAAKNSEADADGWVTVGKEGRNAGFEQKESVIGRLEKKRENDTKTKELKNFYTFQIRESKMQNIVEMRKKFEEDKRKIELLKQSRRFKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZA2</accession>
    <accession>A0A6I8UDV0</accession>
    <name>Q2LZA2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein phosphatase inhibitor 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">I-2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein phosphatase inhibitor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353871.2">
        <property type="nucleotide sequence ID" value="XM_001353835.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZA2"/>
    <dbReference type="FunCoup" id="Q2LZA2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="4813105"/>
    <dbReference type="KEGG" id="dpo:4813105"/>
    <dbReference type="HOGENOM" id="CLU_084310_1_0_1"/>
    <dbReference type="InParanoid" id="Q2LZA2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070462">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZA2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1050">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="180"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353871.2"/>
        </source>
    </evidence>
    <sequence length="224" mass="25448" checksum="E270E32513F3C0BD" modified="2016-01-20" version="2">MPNGYKRGLAPRWVPTREISQRRDKMQNQPSPQLPCKGILKTSRSFDKSGASFRKSAKFDELNVLQTFHPADKDYGHMKIDEPKTPYYEETRDELDTELLVEKLRIAASSQQTSESIEDEGSSGDDLPMSEEERQRRREFERRRKAHYREFEAVKLARKLIQEEDDDDDEDKNAVDTSDSRPSGSQQGSSTSSRYASSSAKKASSNATKSPSTSSNMDLEPSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NV89</accession>
    <accession>A0A6I8V9Y7</accession>
    <name>A0A0R3NV89_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6902739</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036858.1">
        <property type="nucleotide sequence ID" value="XM_015181372.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NV89"/>
    <dbReference type="GeneID" id="6902739"/>
    <dbReference type="KEGG" id="dpo:6902739"/>
    <dbReference type="InParanoid" id="A0A0R3NV89"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250119">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NV89">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015036858.1"/>
        </source>
    </evidence>
    <sequence length="46" mass="5243" checksum="EECF90F1FE5CDF14" modified="2016-01-20" version="1">MSDKNLAAFNAFRRDLARIQKTFSSYTQTDFTQEVGTSTDSENDPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P4L9</accession>
    <accession>A0A6I8VL16</accession>
    <name>A0A0R3P4L9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyrimidodiazepine synthase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GstO2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Omega family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043198.1">
        <property type="nucleotide sequence ID" value="XM_015187712.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P4L9"/>
    <dbReference type="SMR" id="A0A0R3P4L9"/>
    <dbReference type="STRING" id="46245.A0A0R3P4L9"/>
    <dbReference type="GeneID" id="6900706"/>
    <dbReference type="eggNOG" id="KOG0406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3P4L9"/>
    <dbReference type="OMA" id="WYTDYSP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244851">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P4L9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045174">
        <property type="term" value="F:glutathione dehydrogenase (ascorbate) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03184">
        <property type="entry name" value="GST_C_Omega"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000009">
        <property type="entry name" value="Glutathione S-transferase omega-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000123">
        <property type="entry name" value="Glutathione transferase o1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005442">
        <property type="entry name" value="GST_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01625">
        <property type="entry name" value="GSTRNSFRASEO"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015043198.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="28949" checksum="1808C238BC0296F3" modified="2016-01-20" version="1">MSIPQKHFKKGTPKPEIPEDGVLRYYTMRFCPYSERAQLMLAAKKIPHHTVYIDLTEKPEWYIDYSPLGKVPAIQLPHLPGQPTLVESLVIAEYLDEEYPGERSLFPKDPLQKALDRILIERLSPAVSAIYPVFFTTNPPEDALKNFEAALDVFEEEITKRGTPYFGGEQIGIVDYMIWPWFERFPALKYTLAEPYELDSKRYPNLLNWRLLVSQDEAVKATALDAKVHAKFMRTRHEGKTNYDLAFEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P614</accession>
    <accession>A0A6I8VM11</accession>
    <name>A0A0R3P614_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26533572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015043682.1">
        <property type="nucleotide sequence ID" value="XM_015188196.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P614"/>
    <dbReference type="GeneID" id="26533572"/>
    <dbReference type="KEGG" id="dpo:26533572"/>
    <dbReference type="InParanoid" id="A0A0R3P614"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272559">
        <property type="expression patterns" value="Expressed in male reproductive system"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015043682.1"/>
        </source>
    </evidence>
    <sequence length="104" mass="11704" checksum="9E2FC6AE8D4B63E0" modified="2016-01-20" version="1">MGYEFLLEFRSGKFKLRLDPIPNRFNMHRMLLYTLVIVALALVQACHQIHYNLTFPAVNQVCSELSLGQSLTRDLSTMSMSLSVAVSPLAPNLCRHGNPFTLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P8I9</accession>
    <accession>A0A6I8VL14</accession>
    <name>A0A0R3P8I9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-lysine(27) N-trimethyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.356</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">E(z)</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60292"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.356"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.356" evidence="2"/>
    <dbReference type="RefSeq" id="XP_015043165.1">
        <property type="nucleotide sequence ID" value="XM_015187679.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P8I9"/>
    <dbReference type="FunCoup" id="A0A0R3P8I9">
        <property type="interactions" value="1050"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P8I9"/>
    <dbReference type="GeneID" id="4813247"/>
    <dbReference type="InParanoid" id="A0A0R3P8I9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079640">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P8I9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140951">
        <property type="term" value="F:histone H3K27 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19218">
        <property type="entry name" value="SET_EZH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000001">
        <property type="entry name" value="Putative histone-lysine N-methyltransferase EZH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026489">
        <property type="entry name" value="CXC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048358">
        <property type="entry name" value="EZH1/2_MCSS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044439">
        <property type="entry name" value="EZH2_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041343">
        <property type="entry name" value="PRC2_HTH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041355">
        <property type="entry name" value="Pre-SET_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21358">
        <property type="entry name" value="Ezh2_MCSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18118">
        <property type="entry name" value="PRC2_HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18264">
        <property type="entry name" value="preSET_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51633">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="175"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="377"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015043165.1"/>
        </source>
    </evidence>
    <sequence length="754" mass="86171" checksum="082FADEDD1FCAB44" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAQ0</accession>
    <name>A0A6I8UAQ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine-rich cell wall structural protein 1.0</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352465.3">
        <property type="nucleotide sequence ID" value="XM_001352429.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAQ0"/>
    <dbReference type="KEGG" id="dpo:4812201"/>
    <dbReference type="InParanoid" id="A0A6I8UAQ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026197852" evidence="2">
        <location>
            <begin position="22"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352465.3"/>
        </source>
    </evidence>
    <sequence length="363" mass="36498" checksum="542EF17964B8F0FA" modified="2020-08-12" version="1">MRSDCLILALGLVLLVGSVAGKSSSLSDGDRDQWVWRSYNRQQRSFRDGGGDIGRSRSAAIRNSYDNKLRREPTTRRPLPGEPENDEIEDYADAVDPNRASQDTTTPNVGTRQYNPYGGQPNAGQFGGSGLSGGYGGSPGVLVGPGGPTGIIGRQPLYPSPYQPGYGGFNGAQNGIGGYPGGIYGGASGAGLQGYPGASGAGYPGGSLGGAFGGYPSNGLGVGQFSGVGQQFPSGGQFSGVAQQFPGASQFPGSGGQYPFGGSYPGGDFNGNQFAGQFPGNGGQQYPSNQFGGGGPQYTEGYGLAGGLGLGQLGIGGAGGGPYGGGNFGFDEKSSPPVVAEGKSAKSVDAAPRNVNDKLSKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBM7</accession>
    <name>A0A6I8UBM7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="5 16">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 16">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Ltn1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the LTN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 16"/>
    <dbReference type="RefSeq" id="XP_001352950.2">
        <property type="nucleotide sequence ID" value="XM_001352914.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UBM7">
        <property type="interactions" value="2128"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812318"/>
    <dbReference type="InParanoid" id="A0A6I8UBM7"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16491">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000038">
        <property type="entry name" value="E3 ubiquitin-protein ligase listerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054477">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054476">
        <property type="entry name" value="Ltn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054478">
        <property type="entry name" value="LTN1_UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039804">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22958">
        <property type="entry name" value="Ltn1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22999">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23009">
        <property type="entry name" value="UBC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="1694"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001352950.2"/>
        </source>
    </evidence>
    <sequence length="1744" mass="198253" checksum="62356FFB0259BD06" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCP6</accession>
    <name>A0A6I8UCP6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ketohexokinase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353295.3">
        <property type="nucleotide sequence ID" value="XM_001353259.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCP6"/>
    <dbReference type="FunCoup" id="A0A6I8UCP6">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812990"/>
    <dbReference type="InParanoid" id="A0A6I8UCP6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004454">
        <property type="term" value="F:ketohexokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01939">
        <property type="entry name" value="Ketohexokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052562">
        <property type="entry name" value="Ketohexokinase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034093">
        <property type="entry name" value="KHK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42774:SF3">
        <property type="entry name" value="KETOHEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42774">
        <property type="entry name" value="PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="1">
        <location>
            <begin position="31"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353295.3"/>
        </source>
    </evidence>
    <sequence length="371" mass="41872" checksum="2A4D6A901B9C8D33" modified="2020-08-12" version="1">MKKIVVKEFITVKKVRYLPKPPPPPPPPPQRHVLVVGSCTLDLVTVCDVHPTAGVDQRTSEGCWRRGGSASDMCSVLRRLGTACEFLGRLSRARAFEDLLTSFHSMGIDISHCPMSAHQPTHRSIITIRGCETHTILEYSNRRQELTFQQFLGAVDYQMISWVHFEPRSPTETVRMVQAVRDFNERCPEERIILSADLASLKKPSLMVADLVDYVFVRKHLKHANSFMNGHETVWAVRDGLRQVRAEWKKNQPRKTPHPTQGFLPLDPSHCPAPPQDVPTIIYNNYAEGASCLLPDDTFFKVGPHTPQKIVDVIGENETFAAAVIYALLEPKLRLRDALEYGTRAAVFKVQQDGFDGLRCMPKDLIGCYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH30</accession>
    <accession>Q29JF3</accession>
    <name>A0A6I8UH30_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DDB1- and CUL4-associated factor 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">WD repeat-containing protein 68</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">WD repeat-containing protein An11 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4815905</name>
        <name evidence="16 17" type="synonym">wap</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17">MV-25-SWS-2005</strain>
            <strain evidence="16">MV2-25</strain>
            <tissue evidence="16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the WD repeat DCAF7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355291.3">
        <property type="nucleotide sequence ID" value="XM_001355255.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240908.1">
        <property type="nucleotide sequence ID" value="XM_033385017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UH30"/>
    <dbReference type="SMR" id="A0A6I8UH30"/>
    <dbReference type="GeneID" id="4815905"/>
    <dbReference type="KEGG" id="dpo:4815905"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073151">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000049">
        <property type="entry name" value="DDB1-and CUL4-associated factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="262"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001355291.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033240908.1"/>
        </source>
    </evidence>
    <sequence length="343" mass="38528" checksum="CFA15A8785903F1A" modified="2020-08-12" version="1">MSSTAGKRKEIYKYLAPWPLYSMNWSVRPDKRFRLALGSFIEEYNNKVQIISLDEDTSEFSAKSTFDHPYPTTKIMWIPDSKGVYPDLLATSGDYLRVWRAGEPDTRLECVLNNNKNSDFCAPLTSFDWNEVDPNLVGTSSIDTTCTIWGLETGQPHARVYVAGHVKTQLIAHDKEVYDIAFSRAGGGRDMFASVGADGSVRMFDLRHLEHSTIIYEDPAHTALLRLAWNKQDPNYLATVAMDSCEVIILDVRVPCTPVARLSNHRACVNGIAWAPHSSCHICTAGDDHQALIWDIQQMPRAIEDPILAYTAAEGEVNQIQWGATQPDWIAICYNKACEILRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIW1</accession>
    <name>A0A6I8UIW1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="4">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hll</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001356214.3">
        <property type="nucleotide sequence ID" value="XM_001356178.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIW1"/>
    <dbReference type="FunCoup" id="A0A6I8UIW1">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816301"/>
    <dbReference type="InParanoid" id="A0A6I8UIW1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIW1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF32">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="50"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001356214.3"/>
        </source>
    </evidence>
    <sequence length="689" mass="75910" checksum="051D47BDEFFFCDCA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULC5</accession>
    <accession>Q29P72</accession>
    <name>A0A6I8ULC5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transcription elongation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4817869</name>
        <name evidence="19 20" type="synonym">TfIIS</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19 20">MV-25-SWS-2005</strain>
            <strain evidence="19">MV2-25</strain>
            <tissue evidence="19 20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TFS-II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357352.1">
        <property type="nucleotide sequence ID" value="XM_001357316.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235835.1">
        <property type="nucleotide sequence ID" value="XM_033379944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULC5"/>
    <dbReference type="SMR" id="A0A6I8ULC5"/>
    <dbReference type="GeneID" id="4817869"/>
    <dbReference type="KEGG" id="dpo:4817869"/>
    <dbReference type="OMA" id="CHKKNCT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077643">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000001">
        <property type="entry name" value="Probable Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.10:FF:000020">
        <property type="entry name" value="Transcription elongation factor A (SII), 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.30:FF:000008">
        <property type="entry name" value="transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035100">
        <property type="entry name" value="TF_IIS-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006289">
        <property type="entry name" value="TFSII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01385">
        <property type="entry name" value="TFSII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF0">
        <property type="entry name" value="IP08861P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006704">
        <property type="entry name" value="TF_IIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="19 20" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19 20" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="16">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="17">
        <location>
            <begin position="153"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="15">
        <location>
            <begin position="271"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="83"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001357352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033235835.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="34504" checksum="83C142EA8B8D462F" modified="2020-08-12" version="1">MSVEDEVFRIQKKMSKISTSSDGSGQEQALDLLKALQTLNINLEILTKTRIGMTVNELRKSSKDDEVIALAKTLIKNWKRFLASPAPTTPNNSTAAGKDSSNSSASKSSSSSKSASKEKSSSSSSSSSRDKREEKKVSSSQTSFPSGGMTDAVRLKCREMLTTALKMGEVPEGCAEPEEMAAELEDAIYSEFKNTDMKYKNRIRSRVANLKDPKNPGLRGNFMCGAVSAKQLAKMTPEEMASDEMKKLREKFVKEAINDAQLATVQGTKTDLLKCGKCKKRNCTYNQLQTRSADEPMTTFVMCNECGNRWKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULG6</accession>
    <name>A0A6I8ULG6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Choline/ethanolamine kinase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4817935</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357416.4">
        <property type="nucleotide sequence ID" value="XM_001357380.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULG6"/>
    <dbReference type="FunCoup" id="A0A6I8ULG6">
        <property type="interactions" value="486"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817935"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULG6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05156">
        <property type="entry name" value="ChoK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF93">
        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="143"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="383"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001357416.4"/>
        </source>
    </evidence>
    <sequence length="560" mass="64029" checksum="38B5F8C19814E782" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMX6</accession>
    <name>A0A6I8UMX6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein O-GlcNAcase</fullName>
            <ecNumber evidence="7">3.2.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Beta-N-acetylhexosaminidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Beta-hexosaminidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Oga</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + H2O = N-acetyl-D-glucosamine + L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:48876"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12251"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:90838"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="3.2.1.169"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + H2O = L-threonyl-[protein] + N-acetyl-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:48892"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:12252"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:90840"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="3.2.1.169"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.169" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001358051.3">
        <property type="nucleotide sequence ID" value="XM_001358014.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMX6"/>
    <dbReference type="FunCoup" id="A0A6I8UMX6">
        <property type="interactions" value="2467"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800850"/>
    <dbReference type="InParanoid" id="A0A6I8UMX6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102571">
        <property type="term" value="F:[protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/L-threonine O-N-acetyl-alpha-D-glucosaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016231">
        <property type="term" value="F:beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.240:FF:000002">
        <property type="entry name" value="Bifunctional protein NCOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000009">
        <property type="entry name" value="O-GlcNAcase BT_4395"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.240">
        <property type="entry name" value="STAT, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051822">
        <property type="entry name" value="Glycosyl_Hydrolase_84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011496">
        <property type="entry name" value="O-GlcNAcase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13170">
        <property type="entry name" value="O-GLCNACASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13170:SF16">
        <property type="entry name" value="PROTEIN O-GLCNACASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07555">
        <property type="entry name" value="NAGidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52009">
        <property type="entry name" value="GH84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH84" evidence="10">
        <location>
            <begin position="19"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358051.3"/>
        </source>
    </evidence>
    <sequence length="1023" mass="114611" checksum="B8FE76FBD27F7B05" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN04</accession>
    <name>A0A6I8UN04_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tctex1 domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4800782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein light chain Tctex-type family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357997.2">
        <property type="nucleotide sequence ID" value="XM_001357960.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN04"/>
    <dbReference type="FunCoup" id="A0A6I8UN04">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800782"/>
    <dbReference type="InParanoid" id="A0A6I8UN04"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21459">
        <property type="entry name" value="DLC-like_TCTEX1D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF7">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357997.2"/>
        </source>
    </evidence>
    <sequence length="170" mass="18831" checksum="1C786214BF57CD25" modified="2020-08-12" version="1">MAEEQLKEVNANPASTSPVTDEPEKTPMKPNPPTPLSASAADEGQQTEKAVQAAAYSMRPAFGEMFPLPIVKNIMNSVMTEKLKDKTYDKDVAKIWTLEIADEVNQKIASNPKVRRYKHVVQVMLGQELGAGGFYNSRCCWDCDCDTSVSEVFSNTSIFCVCTVFGTYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNX3</accession>
    <name>A0A6I8UNX3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Farnesol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rdhB</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358563.2">
        <property type="nucleotide sequence ID" value="XM_001358526.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNX3"/>
    <dbReference type="FunCoup" id="A0A6I8UNX3">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="4801478"/>
    <dbReference type="KEGG" id="dpo:4801478"/>
    <dbReference type="InParanoid" id="A0A6I8UNX3"/>
    <dbReference type="OMA" id="SYWRNKV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080080">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358563.2"/>
        </source>
    </evidence>
    <sequence length="247" mass="27147" checksum="C7B05BFB247175C5" modified="2020-08-12" version="1">MEHSYWRNKVAVVTGASVGIGASTAVLLANAGMSVVGLARRVRLIETLNDQVKGEGKIFAYECDLNDEQQLTSAFTWIREKFHCIHVLVCNAGILKANFLSESSTKDIKELFDTNVVATASCLREGLKLMAEANVRGHIVVMNSVLGHRIPEVPVPLFSVYPATKHAITALCQTVRQEIHFLKLNIKLTSICPGMVDTDFLSVYSEAVAELPKLQPLEVAKAVLYALDTPDGVQVEDIILQQMRKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNX4</accession>
    <name>A0A6I8UNX4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358562.3">
        <property type="nucleotide sequence ID" value="XM_001358525.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNX4"/>
    <dbReference type="KEGG" id="dpo:4801477"/>
    <dbReference type="InParanoid" id="A0A6I8UNX4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026053502" evidence="2">
        <location>
            <begin position="26"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358562.3"/>
        </source>
    </evidence>
    <sequence length="271" mass="29161" checksum="1CCF86205356FF6C" modified="2020-08-12" version="1">MYPNWLVLPHLALGCLWLCLPLAGGQMFEQQLTNLLNSNRVGGAATGLTIQRLPSQMVEPGRLQQHLQEITGSEDLAPLLLELLGQSGPVWQGQQPGFSQAATNQPQQKQQQPQQQQQQHFFSYENSQAAGQTSQQPQILPGFNGVNVHPASQQQGQQLFRAPKPIQAEQQQQQQLLEQQQYPETEPKIVKAPPVAAVVAPGSAYTATSFQNPSLQHLRPAGTQPVSSPTPATAAPASSSHPSTCSGMNMPCPNYQTTPLLSATALKATAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP17</accession>
    <name>A0A6I8UP17_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metallothionein-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MtnA</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallothionein superfamily. Type 5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358581.2">
        <property type="nucleotide sequence ID" value="XM_001358544.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP17"/>
    <dbReference type="FunCoup" id="A0A6I8UP17">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="GeneID" id="4801502"/>
    <dbReference type="KEGG" id="dpo:4801502"/>
    <dbReference type="InParanoid" id="A0A6I8UP17"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0263429">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000966">
        <property type="entry name" value="Metalthion_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02067">
        <property type="entry name" value="Metallothio_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00872">
        <property type="entry name" value="MTDIPTERA"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0104">Cadmium</keyword>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001358581.2"/>
        </source>
    </evidence>
    <sequence length="40" mass="3918" checksum="9487E07EBCC99ACA" modified="2020-08-12" version="1">MPCPCGSGCKCATQTPKGACNCGTDCKCGGDNKAKCGCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQW3</accession>
    <name>A0A6I8UQW3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphoglycerate mutase</fullName>
            <ecNumber evidence="9">5.4.2.11</ecNumber>
            <ecNumber evidence="9">5.4.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Pglym87</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 9">
            <text>(2R)-3-phospho-glyceroyl phosphate = (2R)-2,3-bisphosphoglycerate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17765"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="EC" id="5.4.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="9"/>
    <dbReference type="EC" id="5.4.2.4" evidence="9"/>
    <dbReference type="RefSeq" id="XP_001359500.4">
        <property type="nucleotide sequence ID" value="XM_001359463.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQW3"/>
    <dbReference type="FunCoup" id="A0A6I8UQW3">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802611"/>
    <dbReference type="InParanoid" id="A0A6I8UQW3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004082">
        <property type="term" value="F:bisphosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000003">
        <property type="entry name" value="2,3-bisphosphoglycerate-dependent phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010713">
        <property type="entry name" value="PRK14115.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="62"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="141"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="168"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="240"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359500.4"/>
        </source>
    </evidence>
    <sequence length="306" mass="34220" checksum="12819A185AB4B7CE" modified="2020-08-12" version="1">MLALAGKPLGSHVFIELMKLCPIFSCSYSNSNSYAYSHCHSKSYSKDSCQEKKGLYKVVMVRHGESEWNQKNLFCGWFDARLSEKGLQEACSAGIALKKAKMEFDVAYTSLLTRAQDTLAAALSASGHKTIPICKTWRLNERHYGGLTGLNKAETAKKFGEEKVKIWRRSFDTPPPPMEKDHKYYDIIVNDPRYCGQLDPKDFPKSESLKLTIERCLPYWNDVIVPQIQEGKRVLVAAHGNSLRGVVKHLEGISDEAIMGLNLPTGIPFVYELDESLKPLGTLKFLGDPETVKKAMEAVANQGKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR90</accession>
    <name>A0A6I8UR90_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">Pak3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <strain evidence="13">MV2-25</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001359445.5">
        <property type="nucleotide sequence ID" value="XM_001359408.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232639.1">
        <property type="nucleotide sequence ID" value="XM_033376748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR90"/>
    <dbReference type="KEGG" id="dpo:4802547"/>
    <dbReference type="OMA" id="CANIDGD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073369">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000759">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000016">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF21">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="12">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="296"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="106"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="122"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="262"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001359445.5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033232639.1"/>
        </source>
    </evidence>
    <sequence length="572" mass="64835" checksum="C868097660D582C1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URS3</accession>
    <name>A0A6I8URS3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Art3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001359888.3">
        <property type="nucleotide sequence ID" value="XM_001359851.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URS3"/>
    <dbReference type="FunCoup" id="A0A6I8URS3">
        <property type="interactions" value="1248"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803089"/>
    <dbReference type="InParanoid" id="A0A6I8URS3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8URS3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049482">
        <property type="entry name" value="ANM3-like_C2H2_Zf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21137">
        <property type="entry name" value="ANM3_C2H2_Zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase 3-like C2H2 zinc finger" evidence="16">
        <location>
            <begin position="79"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="15">
        <location>
            <begin position="241"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="17">
        <location>
            <begin position="358"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001359888.3"/>
        </source>
    </evidence>
    <sequence length="514" mass="59200" checksum="ECC51AABFEB4FB80" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US27</accession>
    <name>A0A6I8US27_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">Ire1</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="29">MV-25-SWS-2005</strain>
            <tissue evidence="29">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001360052.3">
        <property type="nucleotide sequence ID" value="XM_001360015.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US27"/>
    <dbReference type="FunCoup" id="A0A6I8US27">
        <property type="interactions" value="491"/>
    </dbReference>
    <dbReference type="GeneID" id="4803287"/>
    <dbReference type="KEGG" id="dpo:4803287"/>
    <dbReference type="InParanoid" id="A0A6I8US27"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078280">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09769">
        <property type="entry name" value="Luminal_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13982">
        <property type="entry name" value="STKc_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000077">
        <property type="entry name" value="Putative Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.180:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000215">
        <property type="entry name" value="serine/threonine-protein kinase/endoribonuclease IRE1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
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        <property type="entry name" value="Pkinase"/>
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        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PQQ"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
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    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
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    <keyword evidence="12 29" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 25" id="KW-0732">Signal</keyword>
    <keyword evidence="20" id="KW-0804">Transcription</keyword>
    <keyword evidence="18" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
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    <feature type="chain" id="PRO_5026230516" description="non-specific serine/threonine protein kinase" evidence="25">
        <location>
            <begin position="30"/>
            <end position="1083"/>
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        <location>
            <begin position="555"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="27">
        <location>
            <begin position="822"/>
            <end position="950"/>
        </location>
    </feature>
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        <location>
            <begin position="1057"/>
            <end position="1083"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1062"/>
            <end position="1072"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_001360052.3"/>
        </source>
    </evidence>
    <sequence length="1083" mass="120884" checksum="4491C951C2D80028" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USL7</accession>
    <name>A0A6I8USL7_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4803573</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001360243.4"/>
    </dbReference>
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        <property type="interactions" value="1636"/>
    </dbReference>
    <dbReference type="GeneID" id="4803573"/>
    <dbReference type="KEGG" id="dpo:4803573"/>
    <dbReference type="InParanoid" id="A0A6I8USL7"/>
    <dbReference type="OMA" id="ERRTWFQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075305">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase DRS1"/>
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    </dbReference>
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        <property type="entry name" value="Rs1"/>
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    </dbReference>
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        <property type="entry name" value="DEAD_box_RNA_helicase"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
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        <property type="entry name" value="Helicase_C-like"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="188"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="374"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="R3H" evidence="14">
        <location>
            <begin position="540"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="87"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="564"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="756"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="572"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="653"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001360280.2"/>
        </source>
    </evidence>
    <sequence length="785" mass="88473" checksum="DA385BDD877002D4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTQ3</accession>
    <name>A0A6I8UTQ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lysozyme</fullName>
            <ecNumber evidence="2">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4804178</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001360781.3">
        <property type="nucleotide sequence ID" value="XM_001360744.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTQ3"/>
    <dbReference type="FunCoup" id="A0A6I8UTQ3">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804178"/>
    <dbReference type="InParanoid" id="A0A6I8UTQ3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16890">
        <property type="entry name" value="lyz_i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000019">
        <property type="entry name" value="lysozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008597">
        <property type="entry name" value="Invert_lysozyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11195">
        <property type="entry name" value="DESTABILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11195:SF22">
        <property type="entry name" value="LYSOZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05497">
        <property type="entry name" value="Destabilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51909">
        <property type="entry name" value="LYSOZYME_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="4" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026037695" description="lysozyme" evidence="8">
        <location>
            <begin position="23"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="35"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="40"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="92"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608597-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360781.3"/>
        </source>
    </evidence>
    <sequence length="161" mass="18015" checksum="EB4A8FFB5FEBC9AD" modified="2020-08-12" version="1">MSFGVEYMLCLLLAVSPSLVQSQGHVIDKPVTELCLTCICEAISGCNATRICTSPEKGTCGIFRITWAYWVDAGKLTIDGEHPESERAFINCANDPHCAANLVQNYMKKFNQDCNDDGEMDCHDYARIHKLGAYGCQADLPYNYQSVFDECIENYEDQGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTY0</accession>
    <name>A0A6I8UTY0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">AsnRS-m</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001360836.1">
        <property type="nucleotide sequence ID" value="XM_001360799.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTY0"/>
    <dbReference type="FunCoup" id="A0A6I8UTY0">
        <property type="interactions" value="1297"/>
    </dbReference>
    <dbReference type="GeneID" id="4804248"/>
    <dbReference type="KEGG" id="dpo:4804248"/>
    <dbReference type="InParanoid" id="A0A6I8UTY0"/>
    <dbReference type="OMA" id="PEMAFYD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079864">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04318">
        <property type="entry name" value="EcAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000016">
        <property type="entry name" value="Asparagine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="138"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360836.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="52177" checksum="3475569C7E4CA07B" modified="2020-08-12" version="1">MLLIRRFYCSSQRISHIAKSNKRGDSLGIRGWIKNIRRLKNNTFLDIDDGSSSSRFQVVVPRNPETQNLTPGSVISAVGEIQVAPNGNFELHADRVESLAEGHLQDGYPFTPKQKHAPEYVREHLHLRSRVDYVAAQMRIRHKAQKAIHDYMDELDFVQINTPLMTTNDCEGAGEVFRVQPDSEELLKQMARPNVPAEQSYFDSKVFLSVSGQLHLEAMTYGLGNTYCLAPAFRAENSKSPLHLAEFYMFEAELAHLDELEKLAKFIEQMLKSITNRVLETSSEDLHFCQRNANASSDLPWLTVPWTIMSYDDALHVLQANKESLKTPISLDEGFTKDQELFLVSHCGTPVFVVDWPTQQKPFYMKITRNNPDRVHALDLLMPAVGELCGGSLRESDAATLSSHPQFPKDLSWYVDLRKYGGIPTGGFGMGFERYLQLVTGVKNIRDVIPFPRYPHSCKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU77</accession>
    <name>A0A6I8UU77_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Cysteine dioxygenase</fullName>
            <ecNumber evidence="4 12">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4804444</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="12">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="4 12"/>
    <dbReference type="RefSeq" id="XP_001361005.4">
        <property type="nucleotide sequence ID" value="XM_001360968.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU77"/>
    <dbReference type="FunCoup" id="A0A6I8UU77">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804444"/>
    <dbReference type="InParanoid" id="A0A6I8UU77"/>
    <dbReference type="UniPathway" id="UPA00012">
        <property type="reaction ID" value="UER00537"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042412">
        <property type="term" value="P:taurine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000045">
        <property type="entry name" value="Cysteine dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 14" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0883">Thioether bond</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="10">
        <location>
            <begin position="123"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001361005.4"/>
        </source>
    </evidence>
    <sequence length="242" mass="27995" checksum="E9DFF1F9DFF221DC" modified="2020-08-12" version="1">MALSKIDAEIEQVDQEKYLRQATNYYQALEKPLKYGPVIETMDQLITALHREFESNYVNIEMVNHLMLSYKSNPKEWRKYAKFDRYTYTRNLVDAGNGKFNLLILCWGEGHGSSVHDHADSHCFMKMLKGDLREKRYEYPNRPNNTISKSTSHPHSHEGDIDTRELVEIGETPIGVNDVAYINDNLGLHRVENPSHADTSVSLHLYCPPFDTCSVFQDNCKKTTAKVTFWSKYGVRTKLNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUQ0</accession>
    <name>A0A6I8UUQ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sulfotransferase 1E1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4804536</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361084.3">
        <property type="nucleotide sequence ID" value="XM_001361047.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUQ0"/>
    <dbReference type="FunCoup" id="A0A6I8UUQ0">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804536"/>
    <dbReference type="InParanoid" id="A0A6I8UUQ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="45"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361084.3"/>
        </source>
    </evidence>
    <sequence length="313" mass="36796" checksum="934CB3A267E02C74" modified="2020-08-12" version="1">MESERVTPKSYPTGLIDKDWSKRELFFKSNSQRFLDAVHDLEVRDDDVWVVTLPKCGTTWMQELVWLLINDCNFEAALAKDLELRSPFVEFNFNAHGDDSKVFAVHDVDSPRVIKSHLPLPLLPAQLWQKRHKVVYVFRNPLDALVSRYYHGVTYGHNYGKTLAQFIEENLDTDATFRNAIEHAHEFYQLRHEPWLYYTSFERMKKDLRAVVEDLCRFLNKPIVDQPMDQLLKHLSFEEMKKNPTTNHLWEISQVNHKDAGKEKHNFVRRGKVNGYKDELSGEQIEKANHYIQRCLGEKAVTLDELLLLNDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV58</accession>
    <name>A0A6I8UV58_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein Gfi-1b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4804916</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361401.4">
        <property type="nucleotide sequence ID" value="XM_001361364.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV58"/>
    <dbReference type="FunCoup" id="A0A6I8UV58">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804916"/>
    <dbReference type="InParanoid" id="A0A6I8UV58"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UV58">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000630">
        <property type="entry name" value="Zinc finger protein 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MADF" evidence="10">
        <location>
            <begin position="182"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="MADF" evidence="10">
        <location>
            <begin position="298"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="421"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="477"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="506"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="534"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="562"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="124"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361401.4"/>
        </source>
    </evidence>
    <sequence length="604" mass="70001" checksum="F5D0EC31B74B9B81" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV69</accession>
    <name>A0A6I8UV69_DROPS</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4804586</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361123.2">
        <property type="nucleotide sequence ID" value="XM_001361086.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV69"/>
    <dbReference type="FunCoup" id="A0A6I8UV69">
        <property type="interactions" value="512"/>
    </dbReference>
    <dbReference type="GeneID" id="4804586"/>
    <dbReference type="KEGG" id="dpo:4804586"/>
    <dbReference type="InParanoid" id="A0A6I8UV69"/>
    <dbReference type="OMA" id="KTNTAMV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075062">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UV69">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DENN domain containing 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000001">
        <property type="entry name" value="Putative DENN domain-containing protein 1A"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="cDENN_dom"/>
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        <property type="entry name" value="dDENN_dom"/>
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        <property type="entry name" value="DENN_C"/>
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        <property type="entry name" value="DENND1A/B/C"/>
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    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
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        <property type="entry name" value="uDENN_dom"/>
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    <dbReference type="PANTHER" id="PTHR13196">
        <property type="entry name" value="DENN DOMAIN-CONTAINING"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196:SF14">
        <property type="entry name" value="UDENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="22"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="562"/>
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        <location>
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            <end position="646"/>
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        <location>
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            <end position="714"/>
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        <location>
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        <location>
            <begin position="924"/>
            <end position="952"/>
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        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
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        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
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        <location>
            <begin position="674"/>
            <end position="691"/>
        </location>
    </feature>
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        <location>
            <begin position="742"/>
            <end position="756"/>
        </location>
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        <location>
            <begin position="927"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361123.2"/>
        </source>
    </evidence>
    <sequence length="952" mass="104457" checksum="52099769A8EB140C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVG9</accession>
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        <recommendedName>
            <fullName evidence="4 9">Glucose-6-phosphate isomerase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="11 12" type="primary">Pgi</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the GPI family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001361471.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040386.1">
        <property type="nucleotide sequence ID" value="XM_015184900.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVG9"/>
    <dbReference type="SMR" id="A0A6I8UVG9"/>
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    <dbReference type="KEGG" id="dpo:4805043"/>
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    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
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    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
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    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 9 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001361508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015040386.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="62242" checksum="452429594BC391D4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVI3</accession>
    <name>A0A6I8UVI3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Probable cytochrome P450 317a1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Cyp317a1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361455.2">
        <property type="nucleotide sequence ID" value="XM_001361418.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVI3"/>
    <dbReference type="FunCoup" id="A0A6I8UVI3">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="4804981"/>
    <dbReference type="KEGG" id="dpo:4804981"/>
    <dbReference type="InParanoid" id="A0A6I8UVI3"/>
    <dbReference type="OMA" id="RWGGPRD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074544">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001361455.2"/>
        </source>
    </evidence>
    <sequence length="521" mass="60444" checksum="F874F05A621D17AD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVN5</accession>
    <name>A0A6I8UVN5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein saal1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4804867</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SAAL1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361356.2">
        <property type="nucleotide sequence ID" value="XM_001361319.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVN5"/>
    <dbReference type="FunCoup" id="A0A6I8UVN5">
        <property type="interactions" value="601"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804867"/>
    <dbReference type="InParanoid" id="A0A6I8UVN5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052464">
        <property type="entry name" value="Synovial_Prolif_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424:SF23">
        <property type="entry name" value="PROTEIN SAAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424">
        <property type="entry name" value="SERUM AMYLOID A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="441"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001361356.2"/>
        </source>
    </evidence>
    <sequence length="531" mass="59186" checksum="6A9E16F636AD9F6D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVR4</accession>
    <name>A0A6I8UVR4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361389.2">
        <property type="nucleotide sequence ID" value="XM_001361352.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVR4"/>
    <dbReference type="FunCoup" id="A0A6I8UVR4">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804904"/>
    <dbReference type="InParanoid" id="A0A6I8UVR4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361389.2"/>
        </source>
    </evidence>
    <sequence length="275" mass="30646" checksum="AEBC04FACFACB616" modified="2020-08-12" version="1">MAEQKTVDFVWNCAHKLEAQSHVERLLKSHYITTCRKFARHNVQLPEDSFGAARICARCGNQWTDGNYQLHLQPQRLADTAKRKRLVAKLEMAKASQQKGLLSTGARNRAKWLRKHMASNIVVDCAVCQHKTMLSLEKRKGKKRDKANDETIASTTPTALQETAATNQQKGNNKRVLNKDIKAGQKIPQQEKAKAPTTPARLTKSTHPAAAKHKTPKQKNKTKAAQSAPCSGNGTKTQSKTQKQNALLQLAAQLKSHAFKDASKSQQNRLQAFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVU9</accession>
    <name>A0A6I8UVU9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proton-coupled folate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4805179</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361625.2">
        <property type="nucleotide sequence ID" value="XM_001361588.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVU9"/>
    <dbReference type="FunCoup" id="A0A6I8UVU9">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="4805179"/>
    <dbReference type="KEGG" id="dpo:4805179"/>
    <dbReference type="InParanoid" id="A0A6I8UVU9"/>
    <dbReference type="OMA" id="WSVRDYT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080753">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17386">
        <property type="entry name" value="MFS_SLC46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF39">
        <property type="entry name" value="GH23453P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361625.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="63702" checksum="BFA11BA5B80E06DF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW37</accession>
    <name>A0A6I8UW37_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2</fullName>
            <ecNumber evidence="1">2.1.1.296</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">aft</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside)-(ribonucleotide) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside)-(2'-O-methyl-ribonucleotide) in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67024"/>
            <dbReference type="Rhea" id="RHEA-COMP:17169"/>
            <dbReference type="Rhea" id="RHEA-COMP:17170"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167612"/>
            <dbReference type="ChEBI" id="CHEBI:167614"/>
            <dbReference type="EC" id="2.1.1.296"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.296" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001361785.2">
        <property type="nucleotide sequence ID" value="XM_001361748.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW37"/>
    <dbReference type="FunCoup" id="A0A6I8UW37">
        <property type="interactions" value="2458"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805375"/>
    <dbReference type="InParanoid" id="A0A6I8UW37"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004483">
        <property type="term" value="F:methyltransferase cap1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120550">
        <property type="term" value="F:methyltransferase cap2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025807">
        <property type="entry name" value="Adrift-typ_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050851">
        <property type="entry name" value="mRNA_Cap_2O-Ribose_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16121">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16121:SF2">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51614">
        <property type="entry name" value="SAM_MT_ADRIFT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adrift-type SAM-dependent 2'-O-MTase" evidence="8">
        <location>
            <begin position="95"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361785.2"/>
        </source>
    </evidence>
    <sequence length="704" mass="81074" checksum="520D07FE90A628D0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWB2</accession>
    <name>A0A6I8UWB2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Or49b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361635.2">
        <property type="nucleotide sequence ID" value="XM_001361598.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWB2"/>
    <dbReference type="FunCoup" id="A0A6I8UWB2">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805188"/>
    <dbReference type="InParanoid" id="A0A6I8UWB2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF3">
        <property type="entry name" value="ODORANT RECEPTOR 30A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361635.2"/>
        </source>
    </evidence>
    <sequence length="375" mass="43264" checksum="4FE37C71A5F99D94" modified="2020-08-12" version="1">MFEDIQLIYMNVCILRFWALLYDRNVKRYVCICLASFHIFTQVVYMVTTKEGITGIIRNGYMLVLWINTVLRAVLLMQDQDGYVGLIQSLTSAYYDLASEKDPHIAGLLDDLNRQGMLMARGNLFFGVLTSVGFGLYPLSSSERVLPFGSRIPGVDEYTTPYYQLWYVFQMLITPMGCCMYIPYTSLIVAFIMFGIVRCKALQYRLRLLAVRHPAGQRNSRRLAAEIKECIRYQQSIIAYVDKISGLTTMMFLFELLAFSVLLCALLFMLIIVDSTSQLIIICLYINMILAQILALYWYANELREQSLAVATAAYDTEWYTFEIPLRKNILFMIMRAQRPASILLGNVRPITLELFQNLLNTTYTFFTVLKRVYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWM2</accession>
    <name>A0A6I8UWM2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">26S proteasome non-ATPase regulatory subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Rpn8</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361987.3">
        <property type="nucleotide sequence ID" value="XM_001361950.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWM2"/>
    <dbReference type="SMR" id="A0A6I8UWM2"/>
    <dbReference type="FunCoup" id="A0A6I8UWM2">
        <property type="interactions" value="1764"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805613"/>
    <dbReference type="InParanoid" id="A0A6I8UWM2"/>
    <dbReference type="OMA" id="HAMSIKT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077450">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005838">
        <property type="term" value="C:proteasome regulatory particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08062">
        <property type="entry name" value="MPN_RPN7_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000009">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033858">
        <property type="entry name" value="MPN_RPN7_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="11"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361987.3"/>
        </source>
    </evidence>
    <sequence length="343" mass="38671" checksum="C14BB68F06E95482" modified="2020-08-12" version="1">MPSMEVSVNKVIVHPLVLLSVVDHFNRMGKIGNQKRVVGVLLGCWRSKGVLDVSNSFAVPFDEDDKDKSVWFLDHDYLENMYGMFKKVNARERVVGWYHTGPKLHQNDIAINELIRRYCPNSVLVIIDAKPKDLGLPTEAYISVEEVHDDGSPTSKTFEHVPSEIGAEEAEEVGVEHLLRDIKDTTVGSLSQKITNQLMGLKGLNAQLRDIKQYLQRVGDGKMPINHQIVYQLQDIFNLLPDITNDQFTGTMYVKTNDQMLVVYLASMVRSIIALHNLINNKLANRDAEEGKKAIESADSKDAKDKNKDSKDKDNKDTKDKDGKKTEEKSDKSKDDSSKSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWN7</accession>
    <name>A0A6I8UWN7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Syndetin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Vps50</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361775.3">
        <property type="nucleotide sequence ID" value="XM_001361738.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWN7"/>
    <dbReference type="FunCoup" id="A0A6I8UWN7">
        <property type="interactions" value="1620"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805363"/>
    <dbReference type="InParanoid" id="A0A6I8UWN7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019514">
        <property type="entry name" value="Syndetin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040047">
        <property type="entry name" value="VPS50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019515">
        <property type="entry name" value="VPS54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258:SF0">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10474">
        <property type="entry name" value="Syndetin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10475">
        <property type="entry name" value="Vps54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 N-terminal" evidence="6">
        <location>
            <begin position="73"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Syndetin C-terminal" evidence="5">
        <location>
            <begin position="699"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="930"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="938"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="949"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361775.3"/>
        </source>
    </evidence>
    <sequence length="959" mass="109642" checksum="6E469EFDD7B21158" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYZ0</accession>
    <name>A0A6I8UYZ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">trypsin</fullName>
            <ecNumber evidence="10">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sphe</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="10"/>
    <dbReference type="RefSeq" id="XP_002133726.2">
        <property type="nucleotide sequence ID" value="XM_002133690.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYZ0"/>
    <dbReference type="FunCoup" id="A0A6I8UYZ0">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902199"/>
    <dbReference type="InParanoid" id="A0A6I8UYZ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026160105" description="trypsin" evidence="11">
        <location>
            <begin position="24"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="29"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002133726.2"/>
        </source>
    </evidence>
    <sequence length="252" mass="26640" checksum="52E154D381EC8C93" modified="2020-08-12" version="1">MIQTSGLVVALLCLSVLLGGCQAGLKGRILGGDEAGPTDTPYAASLRVDNAHVCGASIVSATKLVTTAHCLYRDGKLIEPSRLTCRVGSTNQYAGGKVVYVESVTVHPDFYQLQNNVAVITLTSALAFTDRIMAVELASSDDDLPAQGSLISVAGWGRTVEGSNSYKIRELTLTVAEEAVCLDAYSAHDKTSFCLAHELKQGTCHGDGGSGAVYNNKLIGLTNFVVGACGSRYPDVFVRVSSFYEWLQPLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ78</accession>
    <name>A0A6I8UZ78_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein CG1339</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Grl43a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133357.2">
        <property type="nucleotide sequence ID" value="XM_002133321.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ78"/>
    <dbReference type="KEGG" id="dpo:6902423"/>
    <dbReference type="InParanoid" id="A0A6I8UZ78"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133357.2"/>
        </source>
    </evidence>
    <sequence length="451" mass="53584" checksum="951C5A7A6ED9727A" modified="2020-08-12" version="1">MEMTRLMDPSSFPINNQRKPLGETPSFPPVMWHKVEYQRQRKRPTHVGYRVFLQATAWLLYGIAYGCNFFRAKWNPCAGRLEERQYNRIVYKLIIILKIVLVASQYLVYFVMLLAVYIHYSLVEKSSAQNFVMGTFSQGIEYNVFRRLVIFLHLKRDRIFVRQTVNEILRISEEIEQKFGLIYHCDASLLGVYFLKLFLLYVHISSYLYKSYFLLINLLYWMLLEYCFMGWFLYQQLLLNWYRNITTFLQRFIDDHKDRQGILGRYHRRLFWLFELHLRINNLHRSINDSLSWLSTSIYLMIFVCSFRLELLIECIMFGEDELENKLYIIADGCLGPVFIPLLYVLLIGLCTDRLRDAELVMQQQIVIIHGLYIRKAPSRTQAIKLLYNEHTSLIIHQKLEPLQNLIILGTTCDRGFAFDYLLTAILTALSFIQYTLSCGDYPINECATHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZH6</accession>
    <name>A0A6I8UZH6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L30-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6902051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133585.3">
        <property type="nucleotide sequence ID" value="XM_002133549.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZH6"/>
    <dbReference type="KEGG" id="dpo:6902051"/>
    <dbReference type="InParanoid" id="A0A6I8UZH6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="3">
        <location>
            <begin position="61"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133585.3"/>
        </source>
    </evidence>
    <sequence length="159" mass="17761" checksum="9357DBC777B5E9A5" modified="2020-08-12" version="1">MQYVFQDSFESVAQHFLFSIQNSAAVASLVNILFNTEHSFATLRIILVDEISRMNYLSACLEVALKDGKYVVGYKQSLKALRGGKAKLVILASNIPSEMKSEMEKYAAETKTRVMRFCGNNFDLGNAIGKYFNVGTLVITDTKVSEHIVHLLAADNREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0C5</accession>
    <name>A0A6I8V0C5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6900352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134748.3">
        <property type="nucleotide sequence ID" value="XM_002134712.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0C5"/>
    <dbReference type="KEGG" id="dpo:6900352"/>
    <dbReference type="InParanoid" id="A0A6I8V0C5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026149802" evidence="2">
        <location>
            <begin position="17"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002134748.3"/>
        </source>
    </evidence>
    <sequence length="269" mass="31135" checksum="A880FC65B5AEA4C9" modified="2020-08-12" version="1">MILYTINFLIFYTASAHNLEVPNYQNPAKKIVQDLIDSWQSPIGYLESRGYLPKDIQYVPDLNFAQKNSLQDWPQNDVSQKQYQESPAKANAENSIREMDPALAETDRLDSRSFDEQDSDELKKMFGRLFSSKNDDTISDEIKSKLDVSWDMNQIKTSALREANKRYEKFCIKRTQNAPLSTDTDEQFDSDIDAEIAELYPSVLSKKEDIKNNDMIVASPDTKLYHLIKGYGKILGNRELGDKAIYKQIHLAQNKTRRSRRRPSDIFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1Q1</accession>
    <name>A0A6I8V1Q1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chaperone protein DnaJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002136565.2">
        <property type="nucleotide sequence ID" value="XM_002136529.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1Q1"/>
    <dbReference type="KEGG" id="dpo:6903952"/>
    <dbReference type="InParanoid" id="A0A6I8V1Q1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V1Q1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052763">
        <property type="entry name" value="DnaJ_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44825">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44825:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="8"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002136565.2"/>
        </source>
    </evidence>
    <sequence length="205" mass="24166" checksum="D78CF6B621548E09" modified="2020-08-12" version="1">MQWRRVGNLYQVLNVPANASDQEIKRAFIELSKKYHPDANSQSCDPERFSRLWEAYNTLHRPRTRKQYDNNLNIKQQRYVQADILQHNVCRDWRQFHTQMRNRQKSKWMGERMAAAPLARSLPLKSAVQDLRSGCRIVNNGTALRFKWCCTGFSLVSGLILISFKLWRGDPWKDRHPILVDTRSTPLRRDEPPSSTMLDLLWVCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1Z5</accession>
    <name>A0A6I8V1Z5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methionyl-tRNA formyltransferase, mitochondrial</fullName>
            <ecNumber evidence="3">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6896881</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Methionyl-tRNA formyltransferase that formylates methionyl-tRNA in mitochondria and is crucial for translation initiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-methionyl-tRNA(fMet) + (6R)-10-formyltetrahydrofolate = N-formyl-L-methionyl-tRNA(fMet) + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:24381"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002137071.2">
        <property type="nucleotide sequence ID" value="XM_002137035.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1Z5"/>
    <dbReference type="FunCoup" id="A0A6I8V1Z5">
        <property type="interactions" value="689"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896881"/>
    <dbReference type="InParanoid" id="A0A6I8V1Z5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12230:FF:000003">
        <property type="entry name" value="methionyl-tRNA formyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="11">
        <location>
            <begin position="98"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl transferase C-terminal" evidence="12">
        <location>
            <begin position="224"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002137071.2"/>
        </source>
    </evidence>
    <sequence length="342" mass="38776" checksum="AED8518E7E9F81F2" modified="2020-08-12" version="1">MLFGRKLANNFYKYKRKAFQSVCLKITRSISTREPSRILFFGTDNFSLPSLQALHKNCSHNLGVVTSFKSPANCVRTYAEREKLPLQRWPITEDQCTDYDLGVVVSFGHMIPAQIINAFPRGMINVHASLLPLWRGAAPIIYAIMKGDARTGVSIMKIEPHHFDIGAVLAQREVPIRRDIYMPELHASLSLLGADLLVDTVNNLSDRLKNAKPQDNATASYAPKITSKITEILWTELSADELYARHRALYGYKNLTTSFLDKQIQLLELRLPEKQLTMQEPGHFIYQRSRKSLLIGCAQGTQLEVLQVRVEGRKPMSAQDFSNGFLRQARSLQFTHNKQVAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V216</accession>
    <name>A0A6I8V216_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">ADAM 17-like protease isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Tace</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002136928.3">
        <property type="nucleotide sequence ID" value="XM_002136892.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V216"/>
    <dbReference type="FunCoup" id="A0A6I8V216">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896714"/>
    <dbReference type="InParanoid" id="A0A6I8V216"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V216">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14246">
        <property type="entry name" value="ADAM17_MPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04270">
        <property type="entry name" value="ZnMc_TACE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000017">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000003">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034025">
        <property type="entry name" value="ADAM10_ADAM17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032029">
        <property type="entry name" value="ADAM17_MPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051489">
        <property type="entry name" value="ADAM_Metalloproteinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702">
        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702:SF6">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16698">
        <property type="entry name" value="ADAM17_MPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13574">
        <property type="entry name" value="Reprolysin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="674"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="16">
        <location>
            <begin position="234"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="15">
        <location>
            <begin position="479"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002136928.3"/>
        </source>
    </evidence>
    <sequence length="743" mass="84663" checksum="18F99105B925D781" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2K7</accession>
    <name>A0A6I8V2K7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Inactive selenide, water dikinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6900318</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135639.2">
        <property type="nucleotide sequence ID" value="XM_002135603.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2K7"/>
    <dbReference type="KEGG" id="dpo:6900318"/>
    <dbReference type="InParanoid" id="A0A6I8V2K7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V2K7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256:SF0">
        <property type="entry name" value="INACTIVE SELENIDE, WATER DIKINASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="SELENIDE, WATER DIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="176"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002135639.2"/>
        </source>
    </evidence>
    <sequence length="358" mass="38610" checksum="AC7AB144D48F6A53" modified="2020-08-12" version="1">MAFNLNPSLGLSLSGCKVPESVVAELLHGPASTTTPGGQRQETELEEMLRGMEISKKPRGGKDQNLAIEFRYPMIDNPYVLGKIIFAEAVGSLCAKGITDCEKTMMLFCRPPDMSKAMVSGLFSVMSDGFRESCMHTDGVQTWASYKSHSTFIVGALASAYWPQHLYVARDNALDGDVLVLTKALGTQVAIDAYESIYDLNRWDLYASFLSKDDVRVAYRQALHSMCLLNRKAANLMHVHNAHGAMAVSGLGILHHANELALVQKEPVSFVIDTLPVIKHTVAAAKGAAGGGVSPLLQGLSPETSGGLLICMPRDDAVAFCKFFDEYGGSPAWIIGTVQSGPKMASIIKNVKIIEVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V356</accession>
    <name>A0A6I8V356_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6896842</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137045.3">
        <property type="nucleotide sequence ID" value="XM_002137009.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V356"/>
    <dbReference type="KEGG" id="dpo:6896842"/>
    <dbReference type="InParanoid" id="A0A6I8V356"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF17">
        <property type="entry name" value="ENDONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="6">
        <location>
            <begin position="106"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137045.3"/>
        </source>
    </evidence>
    <sequence length="338" mass="38284" checksum="4DD37CE3B164224D" modified="2020-08-12" version="1">MIVLQAKADCPLSRAMIEGTNRIFANRDRRGNYALKRVQRVQTGEVLHMICQPNDIVQTTCQQNTNFTRPLPLRCNNPMPATATIVTDATCRGTMYSIGYTINNRRLELYRACYDRTNVRALFTTHTVYGKTFFPARPCTDFSRDNALSAADARTFTVRSIYDAFRRVFGNNQRYIPNNRDVVINRGHLTPSADYLYGDQMCATFKYVNVVPQFKTINDRNWETIERWVRNRIRGGGSLRIKTGAVGTLILPNTARPPVPHRAILGAGTKNPVPEWMYKVVRTSTNRPLAVFLTYNNIYAPRRPSAPRFCTSVPCPMALVNNAAAGFTYCCNATIFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V396</accession>
    <name>A0A6I8V396_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-conjugating enzyme E2C-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137805.2">
        <property type="nucleotide sequence ID" value="XM_002137769.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V396"/>
    <dbReference type="KEGG" id="dpo:6897669"/>
    <dbReference type="InParanoid" id="A0A6I8V396"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019193">
        <property type="entry name" value="UBQ-conj_enz_E2-bd_prot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09814">
        <property type="entry name" value="HECT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137805.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="57270" checksum="033213FC5B89DBD6" modified="2020-08-12" version="1">MPLDQVISELVRSNCLSVVLVKSLDLFKPGTITSDKENEEVIKNIDISPSSIFWIEKNTQHRILVGEYFDFENVHEARGLIDCIFLNLKLCETNFECLMSRTVRVEIKGLRINLVANKPFSLHCSNCAKELSQMRAYRLVAPLPGIAMRVPRDFWDNFEMTKIAHFDQIYYGLNQIVMSANHIKFDTEMIYCPSRRRVLCPGCSRVLGTFLSNAVCLHADALRMGTRVSPAAPLDFNELLGHITPTQLMLRLLKNVDKSRLFLKTVRPDGQIHYLLMMTESKKLTFLRSKLSLSSKLDPKPVNFPDADTSSEWENSMESSSDGIENDDSMNSSSSISIDKDSPNTVDLSSFTPRPPRKMFGKHDGRETERTASTPNKPINYVYLTPYNGCRVQYIFASNDSELRDIQNTVDNWRDNNMRSLRISYSMMIDLVSELNANEHIEAALQNSKPPFRSQPHRCSHIIFETDEEFYATQQQLDEVRVQPTPDENSEINEIIDSDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3X8</accession>
    <name>A0A6I8V3X8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cytochrome P450 4e2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6898148</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138226.1">
        <property type="nucleotide sequence ID" value="XM_002138190.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3X8"/>
    <dbReference type="GeneID" id="6898148"/>
    <dbReference type="KEGG" id="dpo:6898148"/>
    <dbReference type="InParanoid" id="A0A6I8V3X8"/>
    <dbReference type="OMA" id="NIKVRTF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246037">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V3X8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20628">
        <property type="entry name" value="CYP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF203">
        <property type="entry name" value="CYTOCHROME P450 4D1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002138226.1"/>
        </source>
    </evidence>
    <sequence length="526" mass="61240" checksum="576902BD614851CE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V481</accession>
    <name>A0A6I8V481_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MICOS complex subunit MIC13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138471.2">
        <property type="nucleotide sequence ID" value="XM_002138435.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V481"/>
    <dbReference type="GeneID" id="6898429"/>
    <dbReference type="KEGG" id="dpo:6898429"/>
    <dbReference type="InParanoid" id="A0A6I8V481"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246172">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002138471.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16409" checksum="7046694872F6A306" modified="2020-08-12" version="1">MERISRVIPRSKLNMFVVKILKVMGFAGTVLFLNGLGERDVAPALADCQREERDSAPEEVAKDASKNILFDEDDKRDLQRKTDDFKRKYCTKGGICEPPPPKPFSQSIADAWSDTVRTLKKIPAFWSGVARDSGNSICRLFKGDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4B5</accession>
    <name>A0A6I8V4B5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Venom allergen 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6898450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138493.2">
        <property type="nucleotide sequence ID" value="XM_002138457.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4B5"/>
    <dbReference type="FunCoup" id="A0A6I8V4B5">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898450"/>
    <dbReference type="InParanoid" id="A0A6I8V4B5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026152595" evidence="3">
        <location>
            <begin position="25"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="66"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002138493.2"/>
        </source>
    </evidence>
    <sequence length="289" mass="32222" checksum="2B2307B84CDA2B9A" modified="2020-08-12" version="1">MVLLLHVGLAIAMVMIPLLPQGSAYNYCHNDTHFCLAANRQHFMCRLDAELPPVAAYKGTIPDTKKFHAIILKQLNSYRDRLASGFLETRSNKIFPAAKRMRELIWDLELGYMARSHASTVSFKHSECRATQRFPMAGEALGVVGSTMDRRSMSDVLRLAFDRMFDEHLDVKDPESLISNFDGSRDITAGHFSTIVSDRVSRVGCGVAVATECSDDLPQGHCHFLTCHFDFTNIQGSYVYKEGPPATDCQIWEADPSKDYQHLCGNEGGIFGQDHGDEDLSNTTRILGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4R3</accession>
    <name>A0A6I8V4R3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SH3 domain-containing protein Dlish</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Dlish</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138609.1">
        <property type="nucleotide sequence ID" value="XM_002138573.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4R3"/>
    <dbReference type="FunCoup" id="A0A6I8V4R3">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="GeneID" id="6898589"/>
    <dbReference type="KEGG" id="dpo:6898589"/>
    <dbReference type="InParanoid" id="A0A6I8V4R3"/>
    <dbReference type="OMA" id="MQHHANL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245693">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000270">
        <property type="entry name" value="Blast:Tyrosine-protein kinase Src-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000295">
        <property type="entry name" value="Blast:Tyrosine-protein kinase Src-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000222">
        <property type="entry name" value="SH3 domain-containing protein Dlish"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="57"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="187"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="303"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002138609.1"/>
        </source>
    </evidence>
    <sequence length="371" mass="41891" checksum="1BCE447749A74086" modified="2020-08-12" version="1">MAFLCPVRMRRDKKKATSASIERDLPAVGGLGMGRITGSSSIETLVRVGIEKEHGLSPDSKMVVLHDFTPCVDDELEVKRGQLVNILYRENDWVYVIGQDSRQEGFIPFSYCAPCNTQLAELAVKKKLPREHCPEQPIDENMPLLGAENKLDALCDDPIPVANANGSSNNIENSLHVEPECTPFIKEPSGRCIVLYTFIARDENDLSVERGEFVTVLNREDPDWFWIMRSDGQEGFVPAGFIYPADSVRVLQQQKTPLNENNIQHHSTLNAMESILQQGQQPQPLQQQQQPQQLGLGPDDLRYHGTELVMLYDYKAQAPDDLYLSVRRGDWIYADLNNQTVDGWLWAYAPKTRKYGFIPKAYARPPAMTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5Y3</accession>
    <name>A0A6I8V5Y3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Prominin-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">LOC6897674</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736771.3">
        <property type="nucleotide sequence ID" value="XM_003736723.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037949.2">
        <property type="nucleotide sequence ID" value="XM_015182463.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5Y3"/>
    <dbReference type="KEGG" id="dpo:6897674"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF1">
        <property type="entry name" value="PROMININ-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="534"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="856"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_003736771.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015037949.2"/>
        </source>
    </evidence>
    <sequence length="954" mass="109420" checksum="F530DFBB87194ADB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V777</accession>
    <name>A0A6I8V777_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Golgin subfamily A member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4805706</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_004444583.1">
        <property type="nucleotide sequence ID" value="XM_004444526.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V777"/>
    <dbReference type="FunCoup" id="A0A6I8V777">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="GeneID" id="4805706"/>
    <dbReference type="KEGG" id="dpo:4805706"/>
    <dbReference type="InParanoid" id="A0A6I8V777"/>
    <dbReference type="OMA" id="IQVIIAE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070789">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024858">
        <property type="entry name" value="GOLGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043976">
        <property type="entry name" value="GOLGA_cons_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10881:SF46">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10881">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15070">
        <property type="entry name" value="GOLGA2L5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A conserved" evidence="4">
        <location>
            <begin position="284"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Golgin subfamily A conserved" evidence="4">
        <location>
            <begin position="555"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="22"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="538"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="702"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="198"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="390"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="607"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="670"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="554"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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    <sequence length="780" mass="89136" checksum="20985948180F6DAC" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8V7G9</accession>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Drosophila</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
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            <authorList>
                <consortium name="RefSeq"/>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736941.1">
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        <location>
            <begin position="12"/>
            <end position="37"/>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_003736941.1"/>
        </source>
    </evidence>
    <sequence length="194" mass="22087" checksum="969E809DD5CD7516" modified="2020-08-12" version="1">MKYFEFLSLSSVGLSIAILDILIALSILGPSTYYLYFDYLDIDRWEQTNDVEALSAFGTFVSTLVDYLWVREFSQVFYMTATLIIWVKALINLAVACLLVDGIKQKRLICIAPWLINTYLSMTVETGIFVSLELRIDEVDAAVDRRIARSLIFGVFLVLNALLSYGIYALYRKLKSTPNENIEAIQQNPHTFNA</sequence>
</entry>
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    <accession>A0A6I8V7Z1</accession>
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        <submittedName>
            <fullName evidence="4">TOG array regulator of axonemal microtubules protein 1 isoform X1</fullName>
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        <name evidence="4" type="primary">LOC4817755</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Neoptera</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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    </dbReference>
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        <property type="entry name" value="TOG DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <location>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
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        <source>
            <dbReference type="RefSeq" id="XP_015035638.2"/>
        </source>
    </evidence>
    <sequence length="1699" mass="188110" checksum="1C635B7CBFB6D664" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAQ0</accession>
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        <submittedName>
            <fullName evidence="23 24 25">Sodium/calcium exchanger 3 isoform X1</fullName>
        </submittedName>
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        <name evidence="23 24 25 26" type="primary">Calx</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Sophophora</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23 25 26">MV-25-SWS-2005</strain>
            <strain evidence="22">MV2-25</strain>
            <tissue evidence="23 25 26">Whole body</tissue>
        </source>
    </reference>
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        <reaction evidence="19">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:calcium ion import across plasma membrane"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF65">
        <property type="entry name" value="NA_CA-EXCHANGE PROTEIN, ISOFORM G"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16494">
        <property type="entry name" value="Na_Ca_ex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01259">
        <property type="entry name" value="NACAEXCHNGR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="805"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="838"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="877"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="907"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="947"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="21">
        <location>
            <begin position="425"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="21">
        <location>
            <begin position="538"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_015037293.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_015037294.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_015037302.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_015037303.2"/>
        </source>
    </evidence>
    <sequence length="974" mass="109425" checksum="6296AFC164EE4A1A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VB46</accession>
    <name>A0A6I8VB46_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alpha-glucosidase</fullName>
            <ecNumber evidence="2">3.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CD98hc</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="2"/>
    <dbReference type="RefSeq" id="XP_015037543.2">
        <property type="nucleotide sequence ID" value="XM_015182057.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VB46"/>
    <dbReference type="FunCoup" id="A0A6I8VB46">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VB46"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VB46">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015173">
        <property type="term" value="F:aromatic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015180">
        <property type="term" value="F:L-alanine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015190">
        <property type="term" value="F:L-leucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904273">
        <property type="term" value="P:L-alanine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903801">
        <property type="term" value="P:L-leucine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015823">
        <property type="term" value="P:phenylalanine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11329">
        <property type="entry name" value="AmyAc_maltase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042280">
        <property type="entry name" value="SLC3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031984">
        <property type="entry name" value="SLC3A2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46673">
        <property type="entry name" value="4F2 CELL-SURFACE ANTIGEN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46673:SF1">
        <property type="entry name" value="4F2 CELL-SURFACE ANTIGEN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16028">
        <property type="entry name" value="SLC3A2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Solute carrier family 3 member 2 N-terminal" evidence="6">
        <location>
            <begin position="100"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="5">
        <location>
            <begin position="221"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015037543.2"/>
        </source>
    </evidence>
    <sequence length="620" mass="68493" checksum="B87B9A9EAFF63B84" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBG8</accession>
    <name>A0A6I8VBG8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Guanylate cyclase</fullName>
            <ecNumber evidence="3 12">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20 21" type="primary">LOC4802304</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <strain evidence="19">MV2-25</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 12"/>
    <dbReference type="RefSeq" id="XP_015037727.2">
        <property type="nucleotide sequence ID" value="XM_015182241.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037730.2">
        <property type="nucleotide sequence ID" value="XM_015182244.2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802304"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VBG8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
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        <property type="project" value="InterPro"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015038829.2"/>
        </source>
    </evidence>
    <sequence length="968" mass="102759" checksum="0E53DD3AE9AFF81A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VH04</accession>
    <name>A0A6I8VH04_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">SNF-related serine/threonine-protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">SNF1-related kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">Snrk</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">MV-25-SWS-2005</strain>
            <tissue evidence="25">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">May play a role in hematopoietic cell proliferation or differentiation. Potential mediator of neuronal apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_015040677.1">
        <property type="nucleotide sequence ID" value="XM_015185191.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VH04"/>
    <dbReference type="FunCoup" id="A0A6I8VH04">
        <property type="interactions" value="708"/>
    </dbReference>
    <dbReference type="GeneID" id="4805479"/>
    <dbReference type="KEGG" id="dpo:4805479"/>
    <dbReference type="InParanoid" id="A0A6I8VH04"/>
    <dbReference type="OMA" id="RKQHKDH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081097">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14074">
        <property type="entry name" value="STKc_SNRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14339">
        <property type="entry name" value="UBA_SNRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000166">
        <property type="entry name" value="SNF-related serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF45">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="20"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="23">
        <location>
            <begin position="295"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="349"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="512"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="718"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="775"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="532"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="670"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="775"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_015040677.1"/>
        </source>
    </evidence>
    <sequence length="853" mass="95264" checksum="E6CC602309592B1F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIU1</accession>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein unc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">unc</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042269.2">
        <property type="nucleotide sequence ID" value="XM_015186783.2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="dpo:6901084"/>
    <dbReference type="InParanoid" id="A0A6I8VIU1"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VIU1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="775"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="842"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="947"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1099"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="913"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="788"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="850"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1099"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1158"/>
            <end position="1168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015042269.2"/>
        </source>
    </evidence>
    <sequence length="1277" mass="142514" checksum="D1A530287405ACB2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKL2</accession>
    <name>A0A6I8VKL2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein IRSp53 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">IRSp53</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015043335.2">
        <property type="nucleotide sequence ID" value="XM_015187849.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8VKL2"/>
    <dbReference type="FunCoup" id="A0A6I8VKL2">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VKL2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VKL2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07605">
        <property type="entry name" value="I-BAR_IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11779">
        <property type="entry name" value="SH3_Irsp53_BAIAP2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000188">
        <property type="entry name" value="Insulin receptor tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027681">
        <property type="entry name" value="IRSp53/IRTKS/Pinkbar"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14206">
        <property type="entry name" value="BRAIN-SPECIFIC ANGIOGENESIS INHIBITOR 1-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14206:SF7">
        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE 53 KDA, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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            <begin position="1"/>
            <end position="242"/>
        </location>
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            <begin position="343"/>
            <end position="404"/>
        </location>
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            <end position="463"/>
        </location>
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            <end position="542"/>
        </location>
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            <end position="614"/>
        </location>
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        <location>
            <begin position="725"/>
            <end position="948"/>
        </location>
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        <location>
            <begin position="997"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
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            <begin position="438"/>
            <end position="453"/>
        </location>
    </feature>
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            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
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        <location>
            <begin position="531"/>
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        </location>
    </feature>
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        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
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        </location>
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            <end position="759"/>
        </location>
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        <location>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="855"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
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            <dbReference type="PROSITE" id="PS51338"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
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            <dbReference type="RefSeq" id="XP_015043335.2"/>
        </source>
    </evidence>
    <sequence length="1127" mass="125113" checksum="1BD8027C1437455A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMK6</accession>
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            <fullName evidence="3">MD-2-related lipid-recognition domain-containing protein</fullName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">MV2-25</strain>
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
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            <strain evidence="2">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033232290.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20806" checksum="EEC03E10CD028227" modified="2020-08-12" version="1">MYNWHKDFTSIVTCFKISSKFEFTNLKCTALDRKFADFEYCHLRSVNRTYKYMSVKVNLFKTPITKIKVNGALFKRFSGYRPFMYNVTIDACRFLNNTESNPVAGYLYNFFNKYSNMNHTCPYDHDLQVEKLPIEFVNYQTTKVLPFPEGDYLLELHWYAYDINRAVVKVYGTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPN5</accession>
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        <recommendedName>
            <fullName evidence="3">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="2" type="primary">LOC117183375</name>
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        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">MV2-25</strain>
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">MV-25-SWS-2005</strain>
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        </source>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033233001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21125" checksum="59C704C54EF6463B" modified="2020-08-12" version="1">MRVPIIPIRVSIFVAMILILHGSDGLFKYKNIKCKCYEKSYCEFKKCELKVLGRGIVGLNMHVQAKQLPIISVKAILTTFRQFSGYRPFLYNVTVDICGFLKNPKRFPFLCMVHDAIRNFSNFNHSCPYNDDIIIDRMVLNDEMISRMPVPNGFYKLLVAVATEGIWRGEVEAYVEVNLGYDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQ12</accession>
    <name>A0A6I8VQ12_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prolyl 4-hydroxylase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117183365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232958.1">
        <property type="nucleotide sequence ID" value="XM_033377067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQ12"/>
    <dbReference type="KEGG" id="dpo:117183365"/>
    <dbReference type="InParanoid" id="A0A6I8VQ12"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272083">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VQ12">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026169511" description="Prolyl 4-hydroxylase N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Prolyl 4-hydroxylase N-terminal" evidence="2">
        <location>
            <begin position="31"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033232958.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="25073" checksum="29DE43D96BAEC844" modified="2020-08-12" version="1">MWVSLRVRIVLTALSYFFWVEANDGKGSSLDIKGLIDVEEKLLSQMVDYSSVLQKKISLMRRYIALMRVKQTHAEPDREQYMANPLQSYSLIHHMHFDWTTWRRLMEQSLGREQISEIRALMPQISRIEYGTAMQDLIHEPMAAVPFDPVEALELATFAYDKERPDLAEMWLNVTLSGYQKLSPSKKELYKVLSVVKESEVQKLYKKKSDELIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VS16</accession>
    <name>A0A6I8VS16_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="4">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12 13" type="primary">LOC4805690</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9 10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="9 10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001362049.2">
        <property type="nucleotide sequence ID" value="XM_001362012.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233846.1">
        <property type="nucleotide sequence ID" value="XM_033377955.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233847.1">
        <property type="nucleotide sequence ID" value="XM_033377956.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233848.1">
        <property type="nucleotide sequence ID" value="XM_033377957.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233849.1">
        <property type="nucleotide sequence ID" value="XM_033377958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VS16"/>
    <dbReference type="KEGG" id="dpo:4805690"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000370">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF116">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE CG5065-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioester reductase (TE)" evidence="7">
        <location>
            <begin position="125"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="6">
        <location>
            <begin position="467"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001362049.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033233846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033233847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033233848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033233849.1"/>
        </source>
    </evidence>
    <sequence length="620" mass="69746" checksum="D2D9803AB52A237C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSZ8</accession>
    <name>A0A6I8VSZ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein phyllopod</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">phyl</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234043.1">
        <property type="nucleotide sequence ID" value="XM_033378152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VSZ8"/>
    <dbReference type="FunCoup" id="A0A6I8VSZ8">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803879"/>
    <dbReference type="InParanoid" id="A0A6I8VSZ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZAD" evidence="2">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="398"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234043.1"/>
        </source>
    </evidence>
    <sequence length="544" mass="60474" checksum="5D4C99F874E0FB7D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTB8</accession>
    <name>A0A6I8VTB8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphatidylinositol N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GlcNAc-PI synthesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PIG-A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033233899.1">
        <property type="nucleotide sequence ID" value="XM_033378008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTB8"/>
    <dbReference type="FunCoup" id="A0A6I8VTB8">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VTB8"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VTB8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03796">
        <property type="entry name" value="GT4_PIG-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000026">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039507">
        <property type="entry name" value="PIG-A/GPI3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013234">
        <property type="entry name" value="PIGA_GPI_anchor_biosynthesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08288">
        <property type="entry name" value="PIGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIGA GPI anchor biosynthesis" evidence="8">
        <location>
            <begin position="40"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="345"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033233899.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="53678" checksum="A75B69438F6E5DDA" modified="2020-08-12" version="1">MRICMVSDFFYPSIGGVEEHVYNLAQMLLSLGHKIVVLTHAYGDCSGIRYVTHGLKVYYLPIKVCYNQCILPTAVCNVPMLRAVLLRERVEIVHGHSAFSALAHEALMVGSLLGLKVTVFTDHSLFGFADLSAALTNNLLEVNLSMVNHAICVSHIGKENTVLRARVAKHRVSVIPNAVDTALFTPDASQRPSNDTINIVVASRLVYRKGIDLLAGVIPRFKNTHNVNFIIVGDGPKRDLLEEIREKTNMQDRVEIVGAVEHVKVRDVLVRGHIFVNTSLTEAYCMAIVEAASCGLQVVSTSVGGIPEVLPKSLILLAEPDIDAIHSAILVAIDRHQKSISSMIRTPASNGHIPSETDRRGMRRQRRKNHSAPDPVSWSQTGCSTPADESSQIEPVLCPYRCNELVETLYNWEDVALRTVRVYDRVRQERAFTISELVNAVYQHGSWFLGFFVVSHFLVRLLDLWRPRSRVEAAQDVARQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWC1</accession>
    <name>A0A6I8VWC1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein aus isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">aus</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235382.1">
        <property type="nucleotide sequence ID" value="XM_033379491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWC1"/>
    <dbReference type="FunCoup" id="A0A6I8VWC1">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VWC1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VWC1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055470">
        <property type="entry name" value="DUF7042"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055471">
        <property type="entry name" value="DUF7043"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055472">
        <property type="entry name" value="DUF7044"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22255">
        <property type="entry name" value="LP06548P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22255:SF9">
        <property type="entry name" value="LP06548P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23069">
        <property type="entry name" value="DUF7042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23070">
        <property type="entry name" value="DUF7043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23071">
        <property type="entry name" value="DUF7044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7044" evidence="4">
        <location>
            <begin position="446"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7042" evidence="2">
        <location>
            <begin position="606"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7043" evidence="3">
        <location>
            <begin position="751"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="937"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="899"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235382.1"/>
        </source>
    </evidence>
    <sequence length="1127" mass="124453" checksum="5E2E354F5C854118" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXP8</accession>
    <name>A0A6I8VXP8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">galectin</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235870.1">
        <property type="nucleotide sequence ID" value="XM_033379979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXP8"/>
    <dbReference type="FunCoup" id="A0A6I8VXP8">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VXP8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VXP8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016936">
        <property type="term" value="F:galactoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000180">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF185">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="138"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="340"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235870.1"/>
        </source>
    </evidence>
    <sequence length="503" mass="58238" checksum="A0E0597A512749E6" modified="2020-08-12" version="1">MTAIILLVCRSVRELLDLSDAFWQNAFNIDRSLTYYHTKRRLELFRFHCREFWLRQYIKNAESMHRRRDVDFAAIEAGADCNQIIENFDDTAQELHLRFQEAERQHLCQMDYLSDIEIVDGREFNASFYEYSDALPKYSNEQIGKLSEGVSFTITGKIAINCERFSINLVHNNETRDVALHINPRLPQNYIVRNTKVADIWGSEEVSSALPFLLRRGDNFAIQVLITDACYMISVNGNHFAEYAHRIPYIAVKILEVKGDVEDVEMQRIIVENYPQRLPESQAKNIALHIEDGLDEIDANIEEAINIPHEWCLISAPVTHSDSPKSTHSTNDPGLTLPYYGSLPTQSLVEGRCLKIEGRVRLLPHSFYINLQQGRDIWPHPVIAFHLNPRFSKASSGAIGKAVVCRNAWYNGSWAEEERSELDTNFRPGRTFSLAIVCAKESFEVYVNRRFITEFKYKVSPEIVDTVYIQGDLKLWNVTLEHNPMVKGKNVRIYHNPMYYDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXZ0</accession>
    <name>A0A6I8VXZ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC117184041</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235965.1">
        <property type="nucleotide sequence ID" value="XM_033380074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXZ0"/>
    <dbReference type="KEGG" id="dpo:117184041"/>
    <dbReference type="InParanoid" id="A0A6I8VXZ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026143682" description="CUB domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="28"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235965.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="17315" checksum="FA4B7105C35BBDB3" modified="2020-08-12" version="1">MHITFEILSFLLLPILLARGEIQWNEDCNFEQSIDLGASFYFRSPNYPAQYQPETNCKLKAATNWANNQLELKCNIDIPKDKKGCINHRMVVITFVDSVENPKSTRVYCGKREFTETILDPKKYAQMYVEFTSNGNKTGKFACTVSNKGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VY35</accession>
    <name>A0A6I8VY35_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Roundabout homolog 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">robo3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033236015.1">
        <property type="nucleotide sequence ID" value="XM_033380124.1"/>
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    <dbReference type="FunCoup" id="A0A6I8VY35">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817623"/>
    <dbReference type="InParanoid" id="A0A6I8VY35"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000948">
        <property type="entry name" value="Roundabout 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001603">
        <property type="entry name" value="Roundabout 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001167">
        <property type="entry name" value="Roundabout 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002070">
        <property type="entry name" value="roundabout homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000026">
        <property type="entry name" value="roundabout homolog 2 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF246">
        <property type="entry name" value="ROUNDABOUT 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026206893" evidence="11">
        <location>
            <begin position="20"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="869"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="23"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="121"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="215"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="306"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="411"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="521"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="646"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="744"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1051"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1061"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1097"/>
            <end position="1118"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033236015.1"/>
        </source>
    </evidence>
    <sequence length="1346" mass="145521" checksum="11E5DEC2B5597E3A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYS2</accession>
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        <submittedName>
            <fullName evidence="4">Sarcoplasmic calcium-binding protein, beta chain-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC26532607</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033380247.1"/>
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        <property type="component" value="Chromosome 4"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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        <property type="entry name" value="EF_hand_dom"/>
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        <property type="entry name" value="EF-hand_8"/>
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    <dbReference type="SUPFAM" id="SSF47473">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="57"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033236138.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="21622" checksum="BE658C2FFDACA8D2" modified="2020-08-12" version="1">MAYSWDNRVDFVVRYMYDIDNNGFLDQNDFLCMAVRACVVEGKGDCSSSRLDDYKKLMTNLWDEISAIADDDKDGKISNQEFKDAVKKTCVGKKYEEFPQAMRAFIESNFKLLDIDSDGIVGVKEYRYNCITRVAIDDISPIDKAFETLLNDEDRKRGGLSLERYKELYGQFLGNTADNHPAVNLFGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYW9</accession>
    <name>A0A6I8VYW9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="6 16">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4816589</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 17">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="6 16"/>
    <dbReference type="RefSeq" id="XP_033236296.1">
        <property type="nucleotide sequence ID" value="XM_033380405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYW9"/>
    <dbReference type="FunCoup" id="A0A6I8VYW9">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816589"/>
    <dbReference type="InParanoid" id="A0A6I8VYW9"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000003">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000019">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 16 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="416"/>
            <end position="419"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033236296.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="49592" checksum="E078A2800118B547" modified="2020-08-12" version="1">MYSIQLLYHFGDARLLYCQVGAASLLKFASGRLFSSKQKCGDAGKPPKKLSLKNVDVAGKRVFMRVDFNVPMKDGKITNNQRIVAALPSIKYALDNKCKCLVLASHLGRPDGKKNKKFTLEPVAKELQKVLGRPVCFLDDCVGENTLDAVKNPPEGSVLLLENLRFYAEETGSSKDKNKKKIKTDPEKVRQFRTKLAKLGEIYVNDAFGTAHRAHSSMMGEGYKVRAAGFLMDKELEYFAKALQEPVKPFLAILGGAKIADKIPLITNLLNNVSVMIVAGGMSFTFLKVLNSMEIGKSLFDEKGAAMVPEIMAKAQEKKVNIILPVDFVCANKIDKNPERVESVDAKQGIPKEMMGLDVGKISIEIFAGAICASKTIVWNGPPGLFENERFANGTRAMLEAVIGATTRGATTIVGGGDTATACKQFGGADKVSHVSTGGGASLELLEGKVLPGVAALSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3M2</accession>
    <name>A0A6I8W3M2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Protein TIS11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">Tis11</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
            <tissue evidence="9 10 11">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355382.3">
        <property type="nucleotide sequence ID" value="XM_001355346.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237937.1">
        <property type="nucleotide sequence ID" value="XM_033382046.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237938.1">
        <property type="nucleotide sequence ID" value="XM_033382047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W3M2"/>
    <dbReference type="KEGG" id="dpo:4816086"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="366"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="219"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355382.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033237937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033237938.1"/>
        </source>
    </evidence>
    <sequence length="461" mass="50763" checksum="74B95B44B7224105" modified="2020-08-12" version="1">MSATIVQKSHYFECGDIFQTVASDHHQPQQQQQQQHQHQHHQQQTADQLVLDISMEQSQPQQHRLHGALTRTISQPAQQRHPLHQQQIQQQQIQQQQQQQQQQQQQQQQGVASLVTIIENLGNMNLHRKLERTQSEPLPQQPMNISRYKTELCRPFEEAGECKYGEKCQFAHGFHELRNLQRHPKYKTEYCRTFHSVGFCPYGPRCHFVHNADEARAQQQAAAQAAKSGQQQQQQQSSGQQQQQQQGLQQQLQQGQHQGFQKPSQLGSQTSISQSISISQSPFFMPLSPPLSMSTGSDRESPTGSLSLSPTNSLTSFPFHDAMPLQHGYLCMAVGSSGGKHGNGGGSSASSTSSASGLGLGLGMAMAMGSATPPESPNVPISPVHTPPPYEPSTPCSSSSCSSSSMSGASGSGLGGQAKQMLQKSMSTPMQQEEMPRLPVFNRLSSAVEDYKQKLQSSLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W725</accession>
    <name>A0A6I8W725_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="15">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">not</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the peptidase C19 family. UBP8 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="15"/>
    <dbReference type="RefSeq" id="XP_033238449.1">
        <property type="nucleotide sequence ID" value="XM_033382558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W725"/>
    <dbReference type="FunCoup" id="A0A6I8W725">
        <property type="interactions" value="1100"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813627"/>
    <dbReference type="InParanoid" id="A0A6I8W725"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W725">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02660">
        <property type="entry name" value="Peptidase_C19D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000089">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF33">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="18">
        <location>
            <begin position="215"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="17">
        <location>
            <begin position="369"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="71"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="481"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033238449.1"/>
        </source>
    </evidence>
    <sequence length="705" mass="78008" checksum="648908082E19985C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W730</accession>
    <name>A0A6I8W730_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Protein FAM91A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">LOC4813201</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM91 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353928.3">
        <property type="nucleotide sequence ID" value="XM_001353892.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239112.1">
        <property type="nucleotide sequence ID" value="XM_033383221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W730"/>
    <dbReference type="KEGG" id="dpo:4813201"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W730">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039199">
        <property type="entry name" value="FAM91"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028097">
        <property type="entry name" value="FAM91_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028091">
        <property type="entry name" value="FAM91_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441:SF2">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14648">
        <property type="entry name" value="FAM91_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14647">
        <property type="entry name" value="FAM91_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM91 N-terminal" evidence="3">
        <location>
            <begin position="23"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="464"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="362"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="752"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="391"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="408"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="760"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001353928.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033239112.1"/>
        </source>
    </evidence>
    <sequence length="959" mass="107641" checksum="E676054042A146E0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7H1</accession>
    <name>A0A6I8W7H1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4802095</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239313.1">
        <property type="nucleotide sequence ID" value="XM_033383422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W7H1"/>
    <dbReference type="FunCoup" id="A0A6I8W7H1">
        <property type="interactions" value="387"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802095"/>
    <dbReference type="InParanoid" id="A0A6I8W7H1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052097">
        <property type="entry name" value="SET-MYND_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165:SF6">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="8">
        <location>
            <begin position="271"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033239313.1"/>
        </source>
    </evidence>
    <sequence length="893" mass="102062" checksum="50D54951822A6A9E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W861</accession>
    <name>A0A6I8W861_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transposable element Tc1 transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC26532114</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033239564.1">
        <property type="nucleotide sequence ID" value="XM_033383673.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239565.1">
        <property type="nucleotide sequence ID" value="XM_033383674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W861"/>
    <dbReference type="KEGG" id="dpo:26532114"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="40590" checksum="48D03C66E3E266FC" modified="2020-08-12" version="1">MGPRSTIDKRILVITCYQNGYSYGQIGDMVNMARSSVQAIVTRFVRENRVCYKSRKAPNKIFTEHEETMIVLKYQNESISLKDLLEYVKNKFNKTCSLKTIHRVLQAAPLAGDNLPLSTDENNDNNKDFSTIENNNNNEVGSTIENNNNSEVGSIIENIPSRLSFANDHLSKSSDFWDSVIFTGVASFSPMYSKIGLKEHHEMKRKYKGGKLVVWGCISAAGVGNLVFVDREMTKQKHLSILKENVIQHAQQFGIRKFHYYQNNNIDHDADIQYWTIWKCPHVMKPPSYDLDINIVDNLWEILDQNIIFHSIIDENDLKAALTQEWKKITPEMAQNLVRTLPNRLQYIVDKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WB21</accession>
    <name>A0A6I8WB21_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117185058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240605.1">
        <property type="nucleotide sequence ID" value="XM_033384714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WB21"/>
    <dbReference type="KEGG" id="dpo:117185058"/>
    <dbReference type="InParanoid" id="A0A6I8WB21"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240605.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="23030" checksum="2783E2C6DF6BCB6D" modified="2020-08-12" version="1">MNKALPSPNDDVTCAMFSADHCTVAFGTSSGRIHVVAVTKDWQDVNSLRRETLISAHQKPVLACAFSPGDSYRFARLSMRLFRGHRAPIKALAYSVCGRFLVSGGLDDLIIVWDATNERMVHCMTQHNAMIESIGFSHCNNLMVVWGKDCHLSTFHFQLLVKCPEMELSSTKAIIDQLLISTVQTSRNGIFLKSGFADRNRLIAIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDU7</accession>
    <name>A0A6I8WDU7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UPF0518 protein GA25918-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC26532143</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FHIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241563.1">
        <property type="nucleotide sequence ID" value="XM_033385672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDU7"/>
    <dbReference type="FunCoup" id="A0A6I8WDU7">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532143"/>
    <dbReference type="InParanoid" id="A0A6I8WDU7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WDU7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019384">
        <property type="entry name" value="FHIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045669">
        <property type="entry name" value="FHIP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045668">
        <property type="entry name" value="FHIP_KELAA_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705:SF11">
        <property type="entry name" value="FHIP FAMILY PROTEIN CG3558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705">
        <property type="entry name" value="RAI16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19314">
        <property type="entry name" value="DUF5917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19311">
        <property type="entry name" value="KELAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10257">
        <property type="entry name" value="RAI16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHF complex subunit HOOK-interacting protein C-terminal" evidence="3">
        <location>
            <begin position="702"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="625"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="805"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="908"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="632"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="815"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="832"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="866"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="920"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="939"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="970"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033241563.1"/>
        </source>
    </evidence>
    <sequence length="1057" mass="115997" checksum="BDDEB0259E083B1C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-05-03" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9C6Y267</accession>
    <name>A0A9C6Y267_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">NADH-ubiquinone oxidoreductase chain 3</fullName>
            <ecNumber evidence="3 13">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ND3</name>
        <name evidence="15" type="ORF">HCS66_mgp10</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="15"/>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2019-12" db="EMBL/GenBank/DDBJ databases">
            <title>Evolutionary conservation of topologically associating domains and their influences on genome synteny and structural variations in Drosophila.</title>
            <authorList>
                <person name="Liao Y."/>
                <person name="Chakraborty M."/>
                <person name="Zhang X."/>
                <person name="Emerson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">MV2-25</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="submission" date="2020-03" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="NCBI Genome Project"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">MV2-25</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Mitochondrion membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the complex I subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="3 13"/>
    <dbReference type="RefSeq" id="YP_009742599.1">
        <property type="nucleotide sequence ID" value="NC_046603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9C6Y267"/>
    <dbReference type="SMR" id="A0A9C6Y267"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Mitochondrion MT"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1610:FF:000004">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1610">
        <property type="entry name" value="NADH:ubiquinone/plastoquinone oxidoreductase, chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000440">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038430">
        <property type="entry name" value="NDAH_ubi_oxred_su3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058:SF9">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00507">
        <property type="entry name" value="Oxidored_q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 13" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <keyword evidence="10 13" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="YP_009742599.1"/>
        </source>
    </evidence>
    <sequence length="117" mass="13505" checksum="0BA4C93811838A7F" modified="2023-05-03" version="1">MFSIILIASLILTIVTIVMFLASILSKKALIDREKSSPFECGFDPKSSSRLPFSLRFFLITIIFLIFDVEIALILPMIIILKFSNIFIWTFTSIIFILILLIGLYHEWNQGMLNWSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>B5DLN2</accession>
    <accession>A0A6I8UZ30</accession>
    <name>B5DLN2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Farnesol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6902280</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133807.1">
        <property type="nucleotide sequence ID" value="XM_002133771.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DLN2"/>
    <dbReference type="SMR" id="B5DLN2"/>
    <dbReference type="FunCoup" id="B5DLN2">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DLN2"/>
    <dbReference type="GeneID" id="6902280"/>
    <dbReference type="KEGG" id="dpo:6902280"/>
    <dbReference type="eggNOG" id="KOG1205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_1"/>
    <dbReference type="InParanoid" id="B5DLN2"/>
    <dbReference type="OMA" id="RSMQQRD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244490">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133807.1"/>
        </source>
    </evidence>
    <sequence length="247" mass="27193" checksum="E66D49AA27108ABC" modified="2008-10-14" version="1">MDRWQDRVAVVTGASSGIGAATAKALVEAGVVVVGLARRMDRMEALRAELPEEMQGRFHLMHCDVADLDSLTAAFDWVEEQLGGVDILVNNAGCLFAGQLLTQDLEKLEQVWKVNIMGVLNCTRRAFRSMQQRDVAGHVVLINSLTGETIINPPGDELQVLNMYPLTKHGIKAMLEVLRQEFRGFKTKIKITSITPGVTDTEILPRGYDSLPMLRPEDIAAGIIYALATPPHVQVHQLTIKPLGEPF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q24621</accession>
    <accession>Q24620</accession>
    <accession>Q29BG8</accession>
    <name>MLC1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Myosin light chain alkali</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Mlc1</name>
        <name type="ORF">GA18996</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="Mol. Biol. Evol." volume="10" first="769" last="790">
            <title>Conservation of alternative splicing and genomic organization of the myosin alkali light-chain (Mlc1) gene among Drosophila species.</title>
            <authorList>
                <person name="Leicht B.G."/>
                <person name="Lyckegaard E.M.S."/>
                <person name="Benedict C.M."/>
                <person name="Clark A.G."/>
            </authorList>
            <dbReference type="PubMed" id="8355600"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>ALTERNATIVE SPLICING</scope>
        <source>
            <tissue>Flight muscle</tissue>
            <tissue>Head</tissue>
            <tissue>Larva</tissue>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text>Myosin is a hexamer of 2 heavy chains and 4 light chains.</text>
    </comment>
    <comment type="alternative products">
        <event type="alternative splicing"/>
        <isoform>
            <id>Q24621-1</id>
            <name>Larval-adult</name>
            <name>Larval-non-IFM</name>
            <sequence type="displayed"/>
        </isoform>
        <isoform>
            <id>Q24621-2</id>
            <name>Indirect flight muscle</name>
            <name>Pupa</name>
            <name>Adult flight muscle</name>
            <sequence type="described" ref="VSP_003368"/>
        </isoform>
    </comment>
    <dbReference type="EMBL" id="L08052">
        <property type="protein sequence ID" value="AAA28691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="L08052">
        <property type="protein sequence ID" value="AAA28690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL27030.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001357894.2">
        <property type="nucleotide sequence ID" value="XM_001357857.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003736281.1">
        <property type="nucleotide sequence ID" value="XM_003736233.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q24621"/>
    <dbReference type="SMR" id="Q24621"/>
    <dbReference type="FunCoup" id="Q24621">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q24621"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0284283">
        <property type="protein sequence ID" value="FBpp0282721"/>
        <property type="gene ID" value="FBgn0012706"/>
    </dbReference>
    <dbReference type="GeneID" id="4800662"/>
    <dbReference type="KEGG" id="dpo:4800662"/>
    <dbReference type="CTD" id="23209"/>
    <dbReference type="eggNOG" id="KOG0030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_13_3_1"/>
    <dbReference type="InParanoid" id="Q24621"/>
    <dbReference type="OMA" id="SLDCKPT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0012706">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005859">
        <property type="term" value="C:muscle myosin complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000286">
        <property type="entry name" value="Myosin alkali light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000267">
        <property type="entry name" value="Myosin light chain alkali"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF33">
        <property type="entry name" value="MYOSIN LIGHT CHAIN ALKALI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0025">Alternative splicing</keyword>
    <keyword id="KW-0505">Motor protein</keyword>
    <keyword id="KW-0514">Muscle protein</keyword>
    <keyword id="KW-0518">Myosin</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000198710" description="Myosin light chain alkali">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand 1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand 2" evidence="1">
        <location>
            <begin position="80"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="splice variant" id="VSP_003368" description="In isoform Indirect flight muscle." evidence="2">
        <original>QFIQRLMSDPVVFD</original>
        <variation>PFLARMCERPDMLK</variation>
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="155" mass="17546" checksum="28F93D88D6A28D69" modified="1996-11-01" version="1">MADVPKREIENVEFVFEVMGSAGEGIDAVDLGDALRALNLNPTLALIEKMGGTKKRNEKKIKMDEFLPIYSQVKKEKEQGCYEDFIECLKLYDKEENGTMMLAELQHALLALGESLDDEQVETLFADCMDPEDDEGLIPYSQFIQRLMSDPVVFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q28ZA9</accession>
    <name>UNPG_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Homeobox protein unplugged</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">unpg</name>
        <name type="ORF">GA14073</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a regulatory role in neural branching of the tracheae: segment-specific aspects of these neural branching patterns appear to be generated by homeotic regulation of expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL25705" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL25705.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q28ZA9"/>
    <dbReference type="SMR" id="Q28ZA9"/>
    <dbReference type="FunCoup" id="Q28ZA9">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q28ZA9"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0277977">
        <property type="protein sequence ID" value="FBpp0276415"/>
        <property type="gene ID" value="FBgn0074104"/>
    </dbReference>
    <dbReference type="GeneID" id="4804593"/>
    <dbReference type="KEGG" id="dpo:4804593"/>
    <dbReference type="CTD" id="35942"/>
    <dbReference type="eggNOG" id="KOG0489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_571441_0_0_1"/>
    <dbReference type="InParanoid" id="Q28ZA9"/>
    <dbReference type="OMA" id="MSPRNYN"/>
    <dbReference type="PhylomeDB" id="Q28ZA9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074104">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051960">
        <property type="term" value="P:regulation of nervous system development"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000360">
        <property type="entry name" value="Gastrulation brain homeobox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042982">
        <property type="entry name" value="GBX-1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24334">
        <property type="entry name" value="HOMEOBOX PROTEIN GBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24334:SF0">
        <property type="entry name" value="HOMEOBOX PROTEIN UNPLUGGED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0371">Homeobox</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000299500" description="Homeobox protein unplugged">
        <location>
            <begin position="1"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="2">
        <location>
            <begin position="323"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="227"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q4V5A3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="EAL25705.1"/>
        </source>
    </evidence>
    <sequence length="491" mass="52534" checksum="E384B9D8554EA95A" modified="2006-04-04" version="1">MERPALLQNGEHGAVGSLETKTTTRLMPKPFSIESLIATQTPATVSASASIAASPPSPPEDHEQEQEQELSARAMVASSALGLTQFPLYNPWLHGYFAQNHERLTHLIAGGGCYLGSPVGNPFAGKEPSHPHPPPPHALDKSPLPHPLDTRFLPFNASAAAAAASAATPTDLSYRRLAELMNQDYVHNLSVNARLQHMAVAGRLQEDHPSHSLMHLQEPMLLQANPFSPAKSVSHSPVEPTLDVGVDEDYECSGDSSSDISLTLSPMPRNCNRELDKSRNGAYTNSDSEDCSDDEGAHSHHDASGLGGKDSQGNGSGSSSSKSRRRRTAFTSEQLLELEREFHAKKYLSLTERSQIATSLKLSEVQVKIWFQNRRAKWKRVKAGLTSHGLGRNGSASGTKIVVPIPVHVNRFAVRSQHQQLEKMCLSGPKPDLRKKLSTEAMSGFEKFNGGGLGGSAPSSSTASVGAGSVGMGLALGIGVTTPLSLGRSIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q297N8</accession>
    <name>HPS5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BLOC-2 complex member HPS5 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Hermansky-Pudlak syndrome 5 protein homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein pink</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">p</name>
        <name type="ORF">GA22022</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has a role in the biogenesis of eye pigment granules. Eye pigment granules are specialized forms of late endosomes or lysosomes. Biogenesis of pigment granules in the eye requires molecular components required for protein delivery to lysosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HPS5 family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL28167" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL28167.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q297N8"/>
    <dbReference type="SMR" id="Q297N8"/>
    <dbReference type="FunCoup" id="Q297N8">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q297N8"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0285717">
        <property type="protein sequence ID" value="FBpp0284155"/>
        <property type="gene ID" value="FBgn0082007"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3621">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_302332_0_0_1"/>
    <dbReference type="InParanoid" id="Q297N8"/>
    <dbReference type="OMA" id="WARCFEQ"/>
    <dbReference type="PhylomeDB" id="Q297N8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082007">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q297N8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031409">
        <property type="term" value="F:pigment binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006726">
        <property type="term" value="P:eye pigment biosynthetic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006622">
        <property type="term" value="P:protein targeting to lysosome"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000968">
        <property type="entry name" value="Hermansky-Pudlak syndrome 5 protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056499">
        <property type="entry name" value="Beta-prop_HPS5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035431">
        <property type="entry name" value="HPS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056446">
        <property type="entry name" value="TPR_HPS5_insects"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287:SF18">
        <property type="entry name" value="BLOC-2 COMPLEX MEMBER HPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23287">
        <property type="entry name" value="RUBY-EYE2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23756">
        <property type="entry name" value="Beta-prop_HPS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23757">
        <property type="entry name" value="TPR_HPS5_insect"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037475">
        <property type="entry name" value="BLOC-2_complex_Hps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0608">Pigment</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0716">Sensory transduction</keyword>
    <keyword id="KW-0844">Vision</keyword>
    <keyword id="KW-0853">WD repeat</keyword>
    <feature type="chain" id="PRO_0000302765" description="BLOC-2 complex member HPS5 homolog">
        <location>
            <begin position="1"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 1" evidence="2">
        <location>
            <begin position="62"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 2" evidence="2">
        <location>
            <begin position="109"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9VHN9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="EAL28167.1"/>
        </source>
    </evidence>
    <sequence length="830" mass="94782" checksum="0B11E5C2DF976BAF" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D16</accession>
    <accession>A0A6I8UC00</accession>
    <name>Q29D16_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chorion protein S15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Cp15</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Chorion membrane (egg shell) protein; plays a role in protecting the egg from the environment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353097.1">
        <property type="nucleotide sequence ID" value="XM_001353061.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D16"/>
    <dbReference type="FunCoup" id="Q29D16">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29D16"/>
    <dbReference type="GeneID" id="4812718"/>
    <dbReference type="KEGG" id="dpo:4812718"/>
    <dbReference type="eggNOG" id="ENOG502TBSK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2075586_0_0_1"/>
    <dbReference type="InParanoid" id="Q29D16"/>
    <dbReference type="OMA" id="CINANPY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079654">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29D16">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042600">
        <property type="term" value="C:egg chorion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005649">
        <property type="entry name" value="Chorion_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03964">
        <property type="entry name" value="Chorion_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001353097.1"/>
        </source>
    </evidence>
    <sequence length="122" mass="12597" checksum="7CB3DE1A1F640D58" modified="2006-04-04" version="1">MKYLFVCVSLALFAYINASPVYGGNRGGYGGGYGGGNHGGYGGDRVEYEQILVPSYGRGHGNQGYDRPQILRSAPSGSSASAAASAAAAAIAPGRYSQWAVPRYEIDGSYNGPSHGHGRGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EL3</accession>
    <accession>A0A6I8UAT5</accession>
    <name>Q29EL3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cuticle protein 64</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4812538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352550.1">
        <property type="nucleotide sequence ID" value="XM_001352514.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EL3"/>
    <dbReference type="STRING" id="46245.Q29EL3"/>
    <dbReference type="GeneID" id="4812538"/>
    <dbReference type="KEGG" id="dpo:4812538"/>
    <dbReference type="eggNOG" id="ENOG502SYSB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751572_0_0_1"/>
    <dbReference type="InParanoid" id="Q29EL3"/>
    <dbReference type="OMA" id="PYVHAPV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078558">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EL3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007614">
        <property type="entry name" value="Retinin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931">
        <property type="entry name" value="FI02976P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34931:SF3">
        <property type="entry name" value="FI02976P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001352550.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="14040" checksum="66DD1ED2FCC6C262" modified="2006-04-04" version="1">MFKLIALVSALCAVANAGLISPYSHGYGLGYGAALAPAYAPAVISHAPIIKSYAAPIVAHHPVATSYANTYKVATKAYPVVHAAPLVHAAPLIHSAPLVHASPALHTTSTYHGGYGSYGGYGSYGSLGYAGLGHGYLH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IM7</accession>
    <name>CASP8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Caspase-8</fullName>
            <ecNumber>3.4.22.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Death-related ced-3/NEDD2-like protein</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Caspase-8 subunit p15</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Caspase-8 subunit p10</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="3" type="primary">Dredd</name>
        <name type="ORF">GA20387</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Effector of the programmed cell death (PCD) activators rpr, grim and W. May play an apoptotic role in the germline as well as soma. Role in immune response, required to resist Gram-negative bacterial infections by regulating DptA. Fadd interacts with Dredd, Fadd promotes cleavage of Dredd and is necessary and sufficient for enhancing Dredd-induced apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala).</text>
            <dbReference type="EC" id="3.4.22.61"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 15 kDa (caspase-8 subunit p15) and a 10 kDa (caspase-8 subunit p10) subunit. Interacts with the N-terminus of Fadd.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C14A family.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL32626" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.4.22.61"/>
    <dbReference type="EMBL" id="CH379063">
        <property type="protein sequence ID" value="EAL32626.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001355567.1">
        <property type="nucleotide sequence ID" value="XM_001355531.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IM7"/>
    <dbReference type="SMR" id="Q29IM7"/>
    <dbReference type="FunCoup" id="Q29IM7">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IM7"/>
    <dbReference type="MEROPS" id="C14.040"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0275081">
        <property type="protein sequence ID" value="FBpp0273519"/>
        <property type="gene ID" value="FBgn0080382"/>
    </dbReference>
    <dbReference type="GeneID" id="4816060"/>
    <dbReference type="KEGG" id="dpo:4816060"/>
    <dbReference type="CTD" id="31011"/>
    <dbReference type="eggNOG" id="KOG3573">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036904_4_2_1"/>
    <dbReference type="InParanoid" id="Q29IM7"/>
    <dbReference type="OMA" id="HGFEGAV"/>
    <dbReference type="PhylomeDB" id="Q29IM7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080382">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IM7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008656">
        <property type="term" value="F:cysteine-type endopeptidase activator activity involved in apoptotic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000029">
        <property type="entry name" value="Death related ced-3/Nedd2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052039">
        <property type="entry name" value="Caspase-related_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056260">
        <property type="entry name" value="Dredd_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056259">
        <property type="entry name" value="Dredd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576:SF41">
        <property type="entry name" value="CASPASE 14, APOPTOSIS-RELATED CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576">
        <property type="entry name" value="MUCOSA ASSOCIATED LYMPHOID TISSUE LYMPHOMA TRANSLOCATION PROTEIN 1/PARACASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23724">
        <property type="entry name" value="Dredd_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23725">
        <property type="entry name" value="Dredd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0053">Apoptosis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0391">Immunity</keyword>
    <keyword id="KW-0399">Innate immunity</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0788">Thiol protease</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_0000271414" evidence="3">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000271415" description="Caspase-8 subunit p15" evidence="4">
        <location>
            <begin position="243"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000271416" evidence="3">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000271417" description="Caspase-8 subunit p10" evidence="4">
        <location>
            <begin position="416"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q14790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q8IRY7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EAL32626.1"/>
        </source>
    </evidence>
    <sequence length="511" mass="57576" checksum="5DA4C77BB7E2C11C" modified="2006-04-04" version="1" precursor="true">MSGHNILTQLESIDVLDLPYVERDLNFAQLVSLSFLLYGDEHSTATYILQKLLVLARATASDWPHSDILSQYAKSKPQTWRKYLVEALCIIGARQVLRKLGLCWQELRMHYLPHVGSIRVHIHPLLKSLYTICEELTLAQRGRMVLDIKEKNSVGDPLRFYDAEYLEIFLLDWLTRRLIRLGDFNANGSDVQLLIEYFKFNDLHAQATLLVDTVNAYATSSCSPATPNVLLPMDGNGIANESSSPTAAASRPRAKNALQLSRENAGIALIINQQEFYRDANDDYKVYLPPIELPLRNGTDMDKQRLTNVFSMLGYKVEAHDNLDHLSMLHHIRQACKRSLLHESLVVCILSHGFEDAVYGANSVALRISDIENVLCSYENLYEKPKLVVIQACQQEDKENNALPYKINASTKSPCQYLNMLRAMSTVPGFPALRHTQTGSWFIQSLCDAIVEHSNSDHIADILTIVINNVANKRGNKNETMVPWTGGALRQHVYFPRTSATDKVVPDSPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JD9</accession>
    <accession>A0A6I8UGW8</accession>
    <name>Q29JD9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Partitioning defective protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">par-6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAR6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355305.1">
        <property type="nucleotide sequence ID" value="XM_001355269.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JD9"/>
    <dbReference type="SMR" id="Q29JD9"/>
    <dbReference type="FunCoup" id="Q29JD9">
        <property type="interactions" value="466"/>
    </dbReference>
    <dbReference type="GeneID" id="4815868"/>
    <dbReference type="KEGG" id="dpo:4815868"/>
    <dbReference type="eggNOG" id="KOG3606">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040653_1_0_1"/>
    <dbReference type="InParanoid" id="Q29JD9"/>
    <dbReference type="OMA" id="TEQSFDK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079198">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JD9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06403">
        <property type="entry name" value="PB1_Par6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06718">
        <property type="entry name" value="PDZ_Par6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000031">
        <property type="entry name" value="Partitioning defective 6 homolog alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000030">
        <property type="entry name" value="Partitioning defective 6 homolog beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051741">
        <property type="entry name" value="PAR6_homolog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034868">
        <property type="entry name" value="PB1_Par6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14102:SF11">
        <property type="entry name" value="LD29223P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14102">
        <property type="entry name" value="PAR-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="257"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="257"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355305.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="39225" checksum="252335817BBF9277" modified="2006-04-04" version="1">MSKNKINTTSATAIGAETNLIEVKSKFDAEFRRWSFKRNETEQSFDKFAALIEQLHKLANIQFLILYIDPRDNDLLPINNDDNFGRALKTARPLLRVIVQRKDDLNEYSGFGTMKPRNLIGSILLGHTPVKTKAPSISKPHDFRQVSAIIDVDIVPETHRRVRLLKHGSDKPLGFYIRDGTSVRVTSSGLEKQPGIFISRLVPGGLAESTGLLAVNDEVIEVNGIEVAGKTLDQVTDMMVANSSNLIITVKPANQRTLTSTHRGSFSRNSQLSSGSHHTNHTNTSDEIEHDDQDDIVDLTGVTLDESPTGTSSTGNHNNHQPQTTQQSSSPPSHHQQAASNASTIMASDVKDGVLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LR7</accession>
    <accession>A0A6I8UKI0</accession>
    <name>Q29LR7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chitinase-like protein Idgf2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Idgf2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family. IDGF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356912.1">
        <property type="nucleotide sequence ID" value="XM_001356876.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LR7"/>
    <dbReference type="SMR" id="Q29LR7"/>
    <dbReference type="FunCoup" id="Q29LR7">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="GeneID" id="4817493"/>
    <dbReference type="KEGG" id="dpo:4817493"/>
    <dbReference type="eggNOG" id="KOG2806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002833_3_2_1"/>
    <dbReference type="InParanoid" id="Q29LR7"/>
    <dbReference type="OMA" id="QAYSMNE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078217">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LR7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02873">
        <property type="entry name" value="GH18_IDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000007">
        <property type="entry name" value="chitinase-like protein Idgf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000071">
        <property type="entry name" value="Imaginal disc growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015520">
        <property type="entry name" value="IDGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF235">
        <property type="entry name" value="CHITINASE-LIKE PROTEIN IDGF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356912.1"/>
        </source>
    </evidence>
    <sequence length="439" mass="48929" checksum="41AA6C40761B6409" modified="2006-04-04" version="1">MKLLLWLSSVACLLVAGTEAASNLVCYYDSSSYVREGLGKLLNPDLEIALQFCTHLVYGYAGLRADNYQAHSLNENLDIYKHQYSEVTSLKRKYPHLKVLLSVGGDRDIDVEHPNKYVELLEGEKVRQVGFIQSAFSLVKNYGFDGLDLAYQFPRNKPRKVHGDVGSAWKSIKKLFTGDFVVDPQAALHKEQFTAFVRDVKDSLRPDGLLLSLTVLPNVNSTWYFDIPALNGLVDFVNLAAFDFITPARNPEEADFTGPIYHPESKDRLAHYNADFQVNYWLQQGFPSSKLNLGVSTYGNAWKLTPESGLDGVPVVPHTDGPAKEGSQSQKPGLLSYAEICGKLTNPQNQYLKGNDSPLRRVTDPTKRFGTYAFRPVDGAVTEGLWVSYDDPDGASNKAAYARVKNLGGVALFDLSYDDFRGQCTGDKYPLLRAVKYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q29ND2</accession>
    <accession>A0A6I8UJ95</accession>
    <name>Q29ND2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 7">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Fkbp59</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 7"/>
    <dbReference type="RefSeq" id="XP_001356347.2">
        <property type="nucleotide sequence ID" value="XM_001356311.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29ND2"/>
    <dbReference type="SMR" id="Q29ND2"/>
    <dbReference type="FunCoup" id="Q29ND2">
        <property type="interactions" value="803"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29ND2"/>
    <dbReference type="GeneID" id="4817114"/>
    <dbReference type="KEGG" id="dpo:4817114"/>
    <dbReference type="eggNOG" id="KOG0543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013615_13_1_1"/>
    <dbReference type="InParanoid" id="Q29ND2"/>
    <dbReference type="OMA" id="FGAEGNE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078246">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000008">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000013">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000044">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF9">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 8" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356347.2"/>
        </source>
    </evidence>
    <sequence length="440" mass="48884" checksum="E1754D611BEB2620" modified="2008-10-14" version="2">MPEETKKLDLSGDGGVLKEILKEGTGNETPHSGCTVSMHYTGRLVDGTEFDSSVSRNEPFEFALGKGNVIKAFDMGVATMKLGERCFLTCAPNYAYGSAGSPPTIPPDSTLIFELEMLGWKGEDLSPNQDGSIDRIILEPSDKKRSPTDGAFVKAHISGSFEGKVFEERDVEFDYGEGSAIGLVDGVEIALEKMNIGETSRITIKPMYAFGVTGNEAFKIPPNATVEYKVKLIDCGKGLEEWKLSDTERIDEAKVYKEKGTNYFKKENWGLAIKMYTKCKNLLPNNADTNEEVKKLKVATHSNIALCHQKSNDHFEAKVECNAVLALEANNVKALYRRGQCNLIINELEDALEDFQKVIQLEPGNKAAANHVVICRQKIKETKTKEKKLYANMFTKLAANDKETEPPRETDVLSKCGEWSEEDAKREADLTLERDNIIMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NE2</accession>
    <accession>A0A6I8UJ85</accession>
    <name>Q29NE2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translocon-associated protein subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Signal sequence receptor subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4816892</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAP-gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356337.1">
        <property type="nucleotide sequence ID" value="XM_001356301.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NE2"/>
    <dbReference type="FunCoup" id="Q29NE2">
        <property type="interactions" value="1485"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NE2"/>
    <dbReference type="GeneID" id="4816892"/>
    <dbReference type="KEGG" id="dpo:4816892"/>
    <dbReference type="eggNOG" id="KOG4490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092935_0_0_1"/>
    <dbReference type="InParanoid" id="Q29NE2"/>
    <dbReference type="OMA" id="PLWLFWR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079199">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29NE2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009779">
        <property type="entry name" value="SSR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399:SF2">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN TRAP , GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07074">
        <property type="entry name" value="TRAP-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001356337.1"/>
        </source>
    </evidence>
    <sequence length="190" mass="21620" checksum="AE843C2AFC36B7FB" modified="2006-04-04" version="1">MGSGKQQKVQSSGFTKEEELLLQDFSRNVSTKSSALFYGNAFIVSAVPIWLFWRIHNMDLWPSSILFVLVTAASTYLMATAYKNIKFQLKHKIAGRREDAVTREVNRQLGDDKKVTRKEKDERILWKKNEVADYEATTFSIFYNNSIYLAVIIFISFFLLKNSTPFVNYIFSVGLASGALALFSTSAQVN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NT9</accession>
    <name>GNPI_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucosamine-6-phosphate deaminase</fullName>
            <shortName>GlcN6P deaminase</shortName>
            <ecNumber evidence="3">3.5.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glucosamine-6-phosphate isomerase</fullName>
            <shortName evidence="5">GNPI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein oscillin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Gnpda</name>
        <name type="synonym">Gnpda1</name>
        <name evidence="4" type="synonym">Oscillin</name>
        <name type="ORF">GA19983</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible conversion of alpha-D-glucosamine 6-phosphate (GlcN-6P) into beta-D-fructose 6-phosphate (Fru-6P) and ammonium ion, a regulatory reaction step in de novo uridine diphosphate-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) biosynthesis via hexosamine pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL34555" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="3"/>
    <dbReference type="EMBL" id="CH379058">
        <property type="protein sequence ID" value="EAL34555.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NT9"/>
    <dbReference type="SMR" id="Q29NT9"/>
    <dbReference type="FunCoup" id="Q29NT9">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NT9"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0281052">
        <property type="protein sequence ID" value="FBpp0279490"/>
        <property type="gene ID" value="FBgn0079979"/>
    </dbReference>
    <dbReference type="GeneID" id="4817879"/>
    <dbReference type="KEGG" id="dpo:4817879"/>
    <dbReference type="CTD" id="33783"/>
    <dbReference type="eggNOG" id="KOG3148">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049611_0_1_1"/>
    <dbReference type="InParanoid" id="Q29NT9"/>
    <dbReference type="OMA" id="HVITQGI"/>
    <dbReference type="PhylomeDB" id="Q29NT9"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079979">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29NT9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006043">
        <property type="term" value="P:glucosamine catabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000004">
        <property type="entry name" value="Glucosamine-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01241">
        <property type="entry name" value="GlcN6P_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018321">
        <property type="entry name" value="Glucosamine6P_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280:SF5">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01161">
        <property type="entry name" value="GLC_GALNAC_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000328092" description="Glucosamine-6-phosphate deaminase">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for enolization step" evidence="1">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="active site" description="For ring-opening step" evidence="1">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for ring-opening step" evidence="1">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="For ring-opening step" evidence="1">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O88958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="P46926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="4">
        <source>
            <dbReference type="UniProtKB" id="Q9VMP9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="274" mass="30927" checksum="BD14E1CF079F6E67" modified="2006-04-04" version="1">MRLVILETSQSVGKWAAKYVMKRINDFQPGPNRYFVLGLPTGSTPLGMYKELIEFHKQGKVSFQYVKTFNMDEYVGLPRDHQESYHFFMWHNFFKHIDIEPQNVHILNGNAPDLVAECNKFEEQIKEAGGVELFIGGIGPDGHIAFNEPGSSLVSRTRVKTLAQDTLEANARFFDNDMSKVPKQALTVGVGTVMDSKEVMILITGAHKAFALYKAIEEGVNHMWTVSAFQQHANTLMICDEDATLELRVKTVKYFKGILRDIDEGAATQDGYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NZ4</accession>
    <accession>A0A6I8ULG4</accession>
    <name>Q29NZ4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit DAD1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Dad1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DAD/OST2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357430.2">
        <property type="nucleotide sequence ID" value="XM_001357394.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NZ4"/>
    <dbReference type="SMR" id="Q29NZ4"/>
    <dbReference type="FunCoup" id="Q29NZ4">
        <property type="interactions" value="1454"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NZ4"/>
    <dbReference type="GeneID" id="4817967"/>
    <dbReference type="KEGG" id="dpo:4817967"/>
    <dbReference type="eggNOG" id="KOG1746">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111220_2_1_1"/>
    <dbReference type="InParanoid" id="Q29NZ4"/>
    <dbReference type="OMA" id="HIILHIV"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072298">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005588">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357430.2"/>
        </source>
    </evidence>
    <sequence length="113" mass="12659" checksum="20FDA7E9F3FD5F76" modified="2008-10-14" version="2">MVVEITGVISKFYNDYVQNTTKKLKLVDIYLCYILLTGIIQFVYCCLVGTFPFNSFLSGFISTVSCFVLGVCLRLQANPQNKTVFAGISPERGFADFIFAHVILHLVVMNFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZP5</accession>
    <accession>A0A6I8UDE1</accession>
    <name>Q2LZP5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Telomere length and silencing protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4813762</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TLS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353728.1">
        <property type="nucleotide sequence ID" value="XM_001353692.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZP5"/>
    <dbReference type="FunCoup" id="Q2LZP5">
        <property type="interactions" value="2020"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZP5"/>
    <dbReference type="GeneID" id="4813762"/>
    <dbReference type="KEGG" id="dpo:4813762"/>
    <dbReference type="eggNOG" id="KOG3345">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053736_1_0_1"/>
    <dbReference type="InParanoid" id="Q2LZP5"/>
    <dbReference type="OMA" id="NIKTGGM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080725">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010756">
        <property type="entry name" value="Tls1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486:SF2">
        <property type="entry name" value="SPLICING FACTOR C9ORF78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486">
        <property type="entry name" value="TELOMERE LENGTH AND SILENCING PROTEIN 1 TLS1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07052">
        <property type="entry name" value="Hep_59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001353728.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33797" checksum="42104004AD045774" modified="2006-02-21" version="1">MSDAADETKTEGATTKVFFKKSSRKNLRQRKNSDDEEKEEKLTLDDIKERQRLRHRPNGVSLVGLALGKKIAPEEELAIKDPFNVKSGGLVNMATLKSGKMKEAEDPYDVGIGTQFSAETNKRDEDEEMMKYIELELQKRKGGGTDAADNDDGDVNKYLTPEDAALYALPDHLRQSSTHRSEEMLSNQMLNGIPEVDLGIQAKICNIEATEDAKQKLLQDAKNKKDGPSQFVPTNMAVNFMQHNRFNIEENSEQRRRKREEREGKKTAQNHTGPNGVKRATDDYHYDKFRKQFRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZX7</accession>
    <accession>A0A6I8UD89</accession>
    <name>Q2LZX7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DDX42</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">DEAD box protein 42</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC4813688</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX42 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001353646.1">
        <property type="nucleotide sequence ID" value="XM_001353610.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZX7"/>
    <dbReference type="SMR" id="Q2LZX7"/>
    <dbReference type="FunCoup" id="Q2LZX7">
        <property type="interactions" value="3318"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZX7"/>
    <dbReference type="GeneID" id="4813688"/>
    <dbReference type="KEGG" id="dpo:4813688"/>
    <dbReference type="eggNOG" id="KOG0339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_9_0_1"/>
    <dbReference type="InParanoid" id="Q2LZX7"/>
    <dbReference type="OMA" id="DHTWEQT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079574">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZX7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17952">
        <property type="entry name" value="DEADc_DDX42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase DDX42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 20" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="275"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="306"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="510"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="670"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="706"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="785"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001353646.1"/>
        </source>
    </evidence>
    <sequence length="812" mass="89479" checksum="B1FF36B80C04EE3B" modified="2006-02-21" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M050</accession>
    <accession>A0A6I8UD42</accession>
    <name>Q2M050_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin repeat domain-containing protein 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Cnb</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353573.1">
        <property type="nucleotide sequence ID" value="XM_001353537.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M050"/>
    <dbReference type="SMR" id="Q2M050"/>
    <dbReference type="FunCoup" id="Q2M050">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="GeneID" id="4812929"/>
    <dbReference type="KEGG" id="dpo:4812929"/>
    <dbReference type="eggNOG" id="ENOG502RYQ0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392930_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M050"/>
    <dbReference type="OMA" id="HQKQKTR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079057">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M050">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051299">
        <property type="term" value="P:centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902410">
        <property type="term" value="P:mitotic cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902017">
        <property type="term" value="P:regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038923">
        <property type="entry name" value="Centrobin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34439">
        <property type="entry name" value="CENTROBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34439:SF1">
        <property type="entry name" value="CENTROBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="597"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="679"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="247"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="623"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353573.1"/>
        </source>
    </evidence>
    <sequence length="702" mass="79985" checksum="B50580B28A0E86A2" modified="2006-02-21" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0A5</accession>
    <accession>A0A6I8UD67</accession>
    <name>Q2M0A5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Polr2H</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively. Directly interacts with POLR2A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353518.1">
        <property type="nucleotide sequence ID" value="XM_001353482.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0A5"/>
    <dbReference type="SMR" id="Q2M0A5"/>
    <dbReference type="FunCoup" id="Q2M0A5">
        <property type="interactions" value="1578"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0A5"/>
    <dbReference type="GeneID" id="4813059"/>
    <dbReference type="KEGG" id="dpo:4813059"/>
    <dbReference type="eggNOG" id="KOG3400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103864_1_1_1"/>
    <dbReference type="InParanoid" id="Q2M0A5"/>
    <dbReference type="OMA" id="KEDDKGW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070918">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000073">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353518.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="17203" checksum="2074B2DDB0CEC8AC" modified="2006-02-21" version="1">MAGVLFEDIFNVKDMDPEGKKFDRVSRLHCESESFKMDLILDINSWLYPMELGDKFRLVLATTLREDGCPDSGEYNPMEHEGTRADSFEYVMYGKIYRIEGDEAHNEASRLSAYVSFGGLLMRLQGDANNLHGFEVDQHMYLLMKRLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M155</accession>
    <accession>A0A6I8UCB5</accession>
    <name>Q2M155_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4812870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353218.1">
        <property type="nucleotide sequence ID" value="XM_001353182.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M155"/>
    <dbReference type="SMR" id="Q2M155"/>
    <dbReference type="STRING" id="46245.Q2M155"/>
    <dbReference type="GeneID" id="4812870"/>
    <dbReference type="KEGG" id="dpo:4812870"/>
    <dbReference type="eggNOG" id="ENOG502RQAI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_730101_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M155"/>
    <dbReference type="OMA" id="EKDACPR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070475">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M155">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353218.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="43033" checksum="049CBA303ECBEA97" modified="2006-02-21" version="1">MPSHVELTLEQLVRIAVGVPEMTHVNVAVLHSLLNVLLKKLNCQDDLVTIGGFEGKCMERILEQSKYSPLPFDVAAIVPISEQLGKVEQLEKRIADLEKNLECHFKQIRVCNQAKGKKFQSKMWEKYASPCEDLCTTCDEDNKIACSLLANVDFMKKLMRQIAGPILLQMEEVGKRLDKFYETLKEFLRQTEALFQRLEVIKQCIMEIESLRIQLQEYNMTFIGTMEELQDMLDSKLDKVHMPALKMYIRDRFDDIEMRLRMIENKETCPRAAGFINSGLCCISCGSTRVGADVGPQVVGLLPDAPARGHPSVVSKPVHCHKNLVCRSLEKEPTLMVRLKNLDLDVLASRLNRPHHGKVCKLPEANDTIYGLRNSCYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M1C0</accession>
    <accession>A0A6I8UC43</accession>
    <name>Q2M1C0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Gonadal protein gdl</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gdl</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gonadal family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353153.2">
        <property type="nucleotide sequence ID" value="XM_001353117.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M1C0"/>
    <dbReference type="FunCoup" id="Q2M1C0">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M1C0"/>
    <dbReference type="GeneID" id="4813035"/>
    <dbReference type="KEGG" id="dpo:4813035"/>
    <dbReference type="eggNOG" id="KOG4810">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096921_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M1C0"/>
    <dbReference type="OMA" id="CVLNESI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077283">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M1C0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010849">
        <property type="entry name" value="Gonadal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13054">
        <property type="entry name" value="DIGEORGE SYNDROME CRITICAL REGION 6 DGCR6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13054:SF2">
        <property type="entry name" value="PROTEIN DGCR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07324">
        <property type="entry name" value="DGCR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353153.2"/>
        </source>
    </evidence>
    <sequence length="198" mass="23064" checksum="7F3C865C9596CEF2" modified="2008-10-14" version="2">MTDITVSNEGPTNGSEPVAVAEDPFQQPSPEFLQRKIYFLVDQLRTMHAELPENLQTRISYDLLTELANCVLNDSIFVIVKALMELQHETERHLIKMRMQVENEYEIEVSDWRAKIKDPEELLHILGLMKLKHSKKLVESDKKIIEILDQKVNDQQSTLQKAGVPGFYVTENPKEIKIQMFLLDFILRLSRLNYEPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NY01</accession>
    <accession>A0A6I8VGN1</accession>
    <name>A0A0R3NY01_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8 9" type="primary">Ctr1A</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8 9">MV-25-SWS-2005</strain>
            <tissue evidence="7 8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015040895.1">
        <property type="nucleotide sequence ID" value="XM_015185409.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040896.1">
        <property type="nucleotide sequence ID" value="XM_015185410.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040897.1">
        <property type="nucleotide sequence ID" value="XM_015185411.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NY01"/>
    <dbReference type="STRING" id="46245.A0A0R3NY01"/>
    <dbReference type="GeneID" id="4815804"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077825">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NY01">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015040895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015040896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015040897.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="25835" checksum="BD3847F067D150CA" modified="2016-01-20" version="1">MDHAHHTAPNVDHSLHHDHAGMHHDHSGMDSSISPTTTTTDSTTHDMHAVHHHSGGGGGTGTGMEHMMSMAFHFGYNETILFSWWHIDTVSGLIGSMIAIFLLALLYEGLKYYREYLFWKTYNLLEYRPVTGPQRNPEAPRLPSAAAAAPSPVQYVGEVVHKQPPSMLSVNHLYQTLLHILQVTLSFLLMLIFMTYNVWLCLMVVLGAAVGYFLFCWKKSVIVDVTEHCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P7Z2</accession>
    <accession>A0A6I8VKW0</accession>
    <name>A0A0R3P7Z2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-terminal amino-acid N(alpha)-acetyltransferase NatA</fullName>
            <ecNumber evidence="4">2.3.1.255</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">vnc</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-alanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-alanyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50500"/>
            <dbReference type="Rhea" id="RHEA-COMP:12701"/>
            <dbReference type="Rhea" id="RHEA-COMP:12702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64718"/>
            <dbReference type="ChEBI" id="CHEBI:83683"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-cysteinyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-cysteinyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50512"/>
            <dbReference type="Rhea" id="RHEA-COMP:12707"/>
            <dbReference type="Rhea" id="RHEA-COMP:12708"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:65250"/>
            <dbReference type="ChEBI" id="CHEBI:133372"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N-terminal L-seryl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50504"/>
            <dbReference type="Rhea" id="RHEA-COMP:12703"/>
            <dbReference type="Rhea" id="RHEA-COMP:12704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-threonyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-threonyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50516"/>
            <dbReference type="Rhea" id="RHEA-COMP:12709"/>
            <dbReference type="Rhea" id="RHEA-COMP:12710"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64739"/>
            <dbReference type="ChEBI" id="CHEBI:133375"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-terminal L-valyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-valyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50508"/>
            <dbReference type="Rhea" id="RHEA-COMP:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12706"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64741"/>
            <dbReference type="ChEBI" id="CHEBI:133371"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-terminal glycyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50496"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133369"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.255" evidence="4"/>
    <dbReference type="RefSeq" id="XP_015043068.1">
        <property type="nucleotide sequence ID" value="XM_015187582.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P7Z2"/>
    <dbReference type="FunCoup" id="A0A0R3P7Z2">
        <property type="interactions" value="735"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P7Z2"/>
    <dbReference type="GeneID" id="26534411"/>
    <dbReference type="KEGG" id="dpo:26534411"/>
    <dbReference type="eggNOG" id="KOG3235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3P7Z2"/>
    <dbReference type="OMA" id="MSMQNAN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0273398">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P7Z2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000101">
        <property type="entry name" value="N-alpha-acetyltransferase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="155"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015043068.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="22743" checksum="9E8FBA9DE9666BBE" modified="2016-01-20" version="1">MNIRCAKPEDLMTMQHCNLLCLPENYQMKYYFYHGLTWPQLSYVAEDDKGGIVGYVLAKMEEPEPNEESRHGHITSLAVKRSYRRLGLAQKLMNQASQAMVECFNAQYVSLHVRKSNRAALNLYTNSLKFKIIEVEPKYYADGEDAYAMRRDLSEFAEDDDDKPNTKDGANDETTGVDKTHLKAIGSGGHNHHGGHDGHCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAG9</accession>
    <name>A0A6I8UAG9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL46</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L46, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mRpL46</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL46 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352414.2">
        <property type="nucleotide sequence ID" value="XM_001352378.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAG9"/>
    <dbReference type="FunCoup" id="A0A6I8UAG9">
        <property type="interactions" value="370"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812302"/>
    <dbReference type="InParanoid" id="A0A6I8UAG9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAG9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04661">
        <property type="entry name" value="NUDIX_MRP_L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000018">
        <property type="entry name" value="39S ribosomal protein L46, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040008">
        <property type="entry name" value="Ribosomal_mL46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021757">
        <property type="entry name" value="Ribosomal_mL46_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033650">
        <property type="entry name" value="Ribosomal_mL46_NUDIX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L46, MITOCHONDRIAL PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11788">
        <property type="entry name" value="MRP-L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL46 N-terminal" evidence="10">
        <location>
            <begin position="26"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="141"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001352414.2"/>
        </source>
    </evidence>
    <sequence length="261" mass="29705" checksum="04E5FEA66CF81245" modified="2020-08-12" version="1">MLRRFVQVGARQATRAQSTAAAQEKWDLYAGVLVERLPVVSKSLSPVEQQFQDLLWRVEYENSLKSDHELKNERELAQAKLIKQGKVQVDLDDSMTKQTAQDLKDAYVEELKKFQLGSRTTPDDAAKKTSSTDRCLEDTLYLLVQQKLGQQQHMILPQGKRQDGESMRQTAERVLREQCGDDLQVLFYGNAPVGFHKYKYPSNQRAESVGAKVFFYRASLRTGNVGDKKTPGPKYEWLPKDALDGKLTNVAYADSIKKFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAL2</accession>
    <name>A0A6I8UAL2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lebercilin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4812053</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LCA5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352437.4">
        <property type="nucleotide sequence ID" value="XM_001352401.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAL2"/>
    <dbReference type="FunCoup" id="A0A6I8UAL2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812053"/>
    <dbReference type="InParanoid" id="A0A6I8UAL2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAL2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026188">
        <property type="entry name" value="Lebercilin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028933">
        <property type="entry name" value="Lebercilin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16650">
        <property type="entry name" value="C21ORF13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16650:SF6">
        <property type="entry name" value="GH21622P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15619">
        <property type="entry name" value="Lebercilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lebercilin" evidence="5">
        <location>
            <begin position="74"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="327"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="593"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="141"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="392"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="501"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="612"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001352437.4"/>
        </source>
    </evidence>
    <sequence length="645" mass="72146" checksum="0D222F5E450FE504" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAS0</accession>
    <name>A0A6I8UAS0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Signal recognition particle 9 kDa protein</fullName>
            <shortName evidence="9">SRP9</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Srp9</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SRP9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352516.2">
        <property type="nucleotide sequence ID" value="XM_001352480.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAS0"/>
    <dbReference type="FunCoup" id="A0A6I8UAS0">
        <property type="interactions" value="1323"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812140"/>
    <dbReference type="InParanoid" id="A0A6I8UAS0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAS0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045900">
        <property type="term" value="P:negative regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.10:FF:000001">
        <property type="entry name" value="Signal recognition particle 9 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.10">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, srp9/1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009018">
        <property type="entry name" value="Signal_recog_particle_SRP9/14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008832">
        <property type="entry name" value="SRP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039914">
        <property type="entry name" value="SRP9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039432">
        <property type="entry name" value="SRP9_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12834">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12834:SF12">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05486">
        <property type="entry name" value="SRP9-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017029">
        <property type="entry name" value="Signal_recog_particle_SRP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54762">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, SRP9/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP9" evidence="10">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001352516.2"/>
        </source>
    </evidence>
    <sequence length="77" mass="9057" checksum="AB4D3FD97C71728D" modified="2020-08-12" version="1">MVFVKNWEDFEIAAENMYMANPQNCRYTMKYVHSKGHILLKMTDNVKCVQYRAENMPDLKKIEKITSNLVGHMASKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBY2</accession>
    <name>A0A6I8UBY2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 298</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP298 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353037.3">
        <property type="nucleotide sequence ID" value="XM_001353001.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBY2"/>
    <dbReference type="FunCoup" id="A0A6I8UBY2">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812649"/>
    <dbReference type="InParanoid" id="A0A6I8UBY2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBY2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003352">
        <property type="term" value="P:regulation of cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021298">
        <property type="entry name" value="CFAP298"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13238:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13238">
        <property type="entry name" value="PROTEIN C21ORF59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11069">
        <property type="entry name" value="CFAP298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353037.3"/>
        </source>
    </evidence>
    <sequence length="261" mass="29400" checksum="904C42DB668418E5" modified="2020-08-12" version="1">MVVLKVKRGDETLFLYETNVGEKTDSVIRDLVAIYNGQLKVQRVCMEIEELAEHGTMLPSEMIGLNDDQIEELKLKDIWADKCIPSGGFTFNKDPMLRRNGQQPNEAMGKVLANTITKAKAMIDKNLAKSSKTLSLKAVQEAINLLRGAVTIVYPMQLPPHDTIRMEFTNTEDLSGTQASKEVIEPSKAQLWFAGRQIFMGKLLKDYLGNNDKTTVVVKLNQLGEGPPGREAVISEQMRRQMMADSFRRQEELKVRAIFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UC39</accession>
    <name>A0A6I8UC39_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein Tmc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Tmc</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353124.4">
        <property type="nucleotide sequence ID" value="XM_001353088.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UC39">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812746"/>
    <dbReference type="InParanoid" id="A0A6I8UC39"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UC39">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF40">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="571"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1295"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1343"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1410"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="8">
        <location>
            <begin position="1223"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="752"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="814"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="982"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1050"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1515"/>
            <end position="1558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1589"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1771"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1859"/>
            <end position="1994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="754"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="814"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="881"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="907"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="933"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="992"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1023"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1058"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1524"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1589"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1610"/>
            <end position="1636"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1647"/>
            <end position="1667"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1690"/>
            <end position="1699"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1716"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1738"/>
            <end position="1756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1779"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1827"/>
            <end position="1840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1891"/>
            <end position="1901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1904"/>
            <end position="1915"/>
        </location>
    </feature>
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        <location>
            <begin position="1921"/>
            <end position="1932"/>
        </location>
    </feature>
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        <location>
            <begin position="1933"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1980"/>
            <end position="1994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353124.4"/>
        </source>
    </evidence>
    <sequence length="2043" mass="227130" checksum="9BA7F5EC0A0885D7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDE2</accession>
    <name>A0A6I8UDE2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein sens</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sens</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353601.3">
        <property type="nucleotide sequence ID" value="XM_001353565.4"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8UDE2"/>
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    <dbReference type="KEGG" id="dpo:4812895"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002812">
        <property type="entry name" value="Neuroectoderm-expressed 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000148">
        <property type="entry name" value="zinc finger protein Gfi-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000208">
        <property type="entry name" value="zinc finger protein Gfi-1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000432">
        <property type="entry name" value="zinc finger protein Gfi-1b isoform X1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="497"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="525"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="108"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="159"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="376"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="378"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001353601.3"/>
        </source>
    </evidence>
    <sequence length="571" mass="64475" checksum="A369A5DCD6E2499C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDE5</accession>
    <name>A0A6I8UDE5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353732.3">
        <property type="nucleotide sequence ID" value="XM_001353696.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDE5"/>
    <dbReference type="KEGG" id="dpo:4813914"/>
    <dbReference type="InParanoid" id="A0A6I8UDE5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353732.3"/>
        </source>
    </evidence>
    <sequence length="570" mass="63507" checksum="0864AF7C8F7EA1F6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDS3</accession>
    <name>A0A6I8UDS3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Biogenesis of lysosome-related organelles complex 1 subunit 6</fullName>
            <shortName evidence="5">BLOC-1 subunit 6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Pldn</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) involved in pigment granule biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the BLOC1S6 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001353863.4"/>
    </dbReference>
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        <property type="interactions" value="558"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812823"/>
    <dbReference type="InParanoid" id="A0A6I8UDS3"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDS3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017242">
        <property type="entry name" value="BLOC-1_pallidin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028119">
        <property type="entry name" value="Snapin/Pallidin/Snn1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31328">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31328:SF2">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14712">
        <property type="entry name" value="Snapin_Pallidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037609">
        <property type="entry name" value="BLOC-1_complex_pallidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353899.3"/>
        </source>
    </evidence>
    <sequence length="173" mass="19657" checksum="2972A5A7D5F4086A" modified="2020-08-12" version="1">MLKSSNINSVINEQLAAGQTASDQRNHLNSNKNSTSDHSTCWQGNNPDDLGGSLAAVQLSAGVLQIAEPPLDRVRSQLRELIGKQNKIYIDLSKEKYKLDCSEVAKLNDMMNDVKRYKEKLTRVKREMQGIYQRTKELKKRAANVAACKQRDYQRKLERQQHEESLIGGQKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE36</accession>
    <name>A0A6I8UE36_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS26</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S26, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mRpS26</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS26 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353967.2">
        <property type="nucleotide sequence ID" value="XM_001353931.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE36"/>
    <dbReference type="FunCoup" id="A0A6I8UE36">
        <property type="interactions" value="424"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813376"/>
    <dbReference type="InParanoid" id="A0A6I8UE36"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026140">
        <property type="entry name" value="Ribosomal_mS26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21035">
        <property type="entry name" value="28S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21035:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14943">
        <property type="entry name" value="MRP-S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353967.2"/>
        </source>
    </evidence>
    <sequence length="225" mass="26248" checksum="188939D2B6BDF4B0" modified="2020-08-12" version="1">MLRAGCQLLTQSTLPAVKTGSNGFALEFVRWRRKPRWLPVAKSKIFRVPERKKQTEEERTELMRLHNRYKTQLRSVRQYLREEVVRHEETSTVDHIVLTPEQEEAEYQALIAANAEWNATIAREREQRLAKERDEKVAYVHERLEARQLRDEERRERANEAVLREIERSKTYITREGLNAAIETALANPVDPNFAIDMAGNLYQGRTTSQLPDADPQPDQQALSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UER5</accession>
    <name>A0A6I8UER5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein RhoGAP15B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RhoGAP15B</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354329.4">
        <property type="nucleotide sequence ID" value="XM_001354293.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UER5">
        <property type="interactions" value="330"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814208"/>
    <dbReference type="InParanoid" id="A0A6I8UER5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048699">
        <property type="term" value="P:generation of neurons"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17113">
        <property type="entry name" value="RA_ARAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04385">
        <property type="entry name" value="RhoGAP_ARAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052227">
        <property type="entry name" value="Arf-Rho-GAP_ANK-PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037858">
        <property type="entry name" value="RhoGAP_ARAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45899">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 15B, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45899:SF2">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 15B, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="1145"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="177"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="273"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="382"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="679"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="771"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="432"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="774"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="816"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001354329.4"/>
        </source>
    </evidence>
    <sequence length="1595" mass="178337" checksum="4A2609DE2B748D91" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH81</accession>
    <name>A0A6I8UH81_DROPS</name>
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        <submittedName>
            <fullName evidence="9">Probable ATP-dependent RNA helicase DHX34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4815424</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355486.3">
        <property type="nucleotide sequence ID" value="XM_001355450.4"/>
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        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815424"/>
    <dbReference type="InParanoid" id="A0A6I8UH81"/>
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        <property type="component" value="Chromosome X"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17979">
        <property type="entry name" value="DEXHc_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000005">
        <property type="entry name" value="ATP-dependent helicase HrpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004714">
        <property type="entry name" value="DEAD/DEAH box helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000725">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056382">
        <property type="entry name" value="DHX34_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24485">
        <property type="entry name" value="zf-C2H2_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="149"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="357"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1071"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1081"/>
            <end position="1090"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355486.3"/>
        </source>
    </evidence>
    <sequence length="1123" mass="128731" checksum="7AE0A11AE9B107E0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UI11</accession>
    <name>A0A6I8UI11_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-deoxynucleotidase HDDC2</fullName>
            <ecNumber evidence="8">3.1.3.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">HD domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC4816265</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HDDC2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="8"/>
    <dbReference type="RefSeq" id="XP_001355799.3">
        <property type="nucleotide sequence ID" value="XM_001355763.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UI11"/>
    <dbReference type="FunCoup" id="A0A6I8UI11">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816265"/>
    <dbReference type="InParanoid" id="A0A6I8UI11"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UI11">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000011">
        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="15">
        <location>
            <begin position="211"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="53"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="385"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="388"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00015933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001355799.3"/>
        </source>
    </evidence>
    <sequence length="413" mass="45206" checksum="322C3E636B3D4DE1" modified="2020-08-12" version="1">MFSLQQIIKAATANSGNCGVGGSDLYRLLRHLMTPSAATTAITTTTTTNTAIPKKSSQATAVSSDENFSHNAIRSSHSNPNGCCYGESDSESAHSVNGNLSTQFARKLLQQRRQDIQRLIHTQPPSGHGTKSSQQQTTTKFAMSSLAAEPLPQAHKPDEITAAASTSDVAASGTTPSNGLGASCQEAASSTGKKPCFSTGLAEILQFMELIGNLKHTKRTGWVLRDVNDCESISGHMYRMSMLTFLLDGSEGLNQIRCMELALVHDLAESLVGDITPFCGVSKEEKRAMEFKAMEDICKLIEPRGKRIMELFEEYEHAESAESRFVKDLDRLDMVMQAFEYEKRDNCLLKHQEFFDSTEGKFNHPFVKKLVNEIYEQRNVLAKAKGATPPPAIEVPKMELPPPKLANGHDSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJS4</accession>
    <name>A0A6I8UJS4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">Polyprenal reductase</fullName>
            <ecNumber evidence="2 9">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4817473</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8 9">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 9">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.94" evidence="2 9"/>
    <dbReference type="RefSeq" id="XP_001356599.3">
        <property type="nucleotide sequence ID" value="XM_001356563.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJS4"/>
    <dbReference type="FunCoup" id="A0A6I8UJS4">
        <property type="interactions" value="1120"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817473"/>
    <dbReference type="InParanoid" id="A0A6I8UJS4"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003865">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016095">
        <property type="term" value="P:polyprenol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="207"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356599.3"/>
        </source>
    </evidence>
    <sequence length="323" mass="37111" checksum="E52C15AD3BA4A1C2" modified="2020-08-12" version="1">MVLAIISSLEELIDQYNVNLTQLVFCIFITTIVFFGSLMTLVESHLPNSIRQSFRYGKHRHKGEADALVSYLEVPKGWFKHFYIFAFGWSLLALYLIVSSIASQSAAPEYALGFLDLVCGGATNRKVQVDSTTALLGTVLLTLQCIRRFYETNFVQIFSKHSKMNLSHYAVGYVHYFGAIVSLLSNTAGFVRGSQPTEFTPKNLSQIQIIYFLVFMFAWHQQYSSNMILVNLRKDAKTGAVKTEQHLLPKGGWFNLLSSPHMFFEVVMYFCLADLFTPVRTWKLVFLWVASNQTINALLTHQWYKETFKDYPKRRRAILPYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULI2</accession>
    <name>A0A6I8ULI2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 16">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Hacd2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Endoplasmic reticulum membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the p23/wos2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 16"/>
    <dbReference type="RefSeq" id="XP_001357322.2">
        <property type="nucleotide sequence ID" value="XM_001357286.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULI2"/>
    <dbReference type="FunCoup" id="A0A6I8ULI2">
        <property type="interactions" value="842"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817946"/>
    <dbReference type="InParanoid" id="A0A6I8ULI2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULI2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06465">
        <property type="entry name" value="p23_hB-ind1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000013">
        <property type="entry name" value="Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF35">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13 16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="17">
        <location>
            <begin position="3"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001357322.2"/>
        </source>
    </evidence>
    <sequence length="370" mass="43437" checksum="7E94E0CFA007798F" modified="2020-08-12" version="1">MAQLSPLVYWSQTKQTLQLKVDLKDAKGAIADFSPVSVAFSANGHGARGVNAYKFDLHFYALIDDENGTFVVNDNKIELQIRKLEPAWWPRLVATPQKPHWLKIDFDRWRTEDDVELEEAPRDVRQDYEKEYKDLQRRELGYVKEKTKKVYLMFYNLAMFVGYLYVVSVMGVLYYRDGSHSITKTYAHVGNAFKFIQLLQYLEVMHPLFGYTKGSPLVPFFQVSGRNFILFLMIELEPRMHAKPVVFYVFIIWSLVELIRYPFYLSQLLGREVGLLTWLRYTIWIPLYPMGIVCEGIIVLRNIPYIEETKRFTVEMPNKLNCTFDMVLFLKLYLLLLILPGTYLVMSHMSKLRAKKLGRGRGKRRQLHAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULT3</accession>
    <name>A0A6I8ULT3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Keratin-associated protein 19-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4800367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357548.1">
        <property type="nucleotide sequence ID" value="XM_001357511.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULT3"/>
    <dbReference type="GeneID" id="4800367"/>
    <dbReference type="KEGG" id="dpo:4800367"/>
    <dbReference type="InParanoid" id="A0A6I8ULT3"/>
    <dbReference type="OMA" id="APYSHLW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026090132" evidence="1">
        <location>
            <begin position="25"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357548.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="14557" checksum="4C630B4DC194E1E1" modified="2020-08-12" version="1">MRIRAPLLMILTLLIASCLDWGSALFLKSWKKYKGSGGVQNYGNYGYGNSGYGYNGYGNSGYGNYGYGKYAGGYGYGYPSYQSNGYRGGRRPSSGQRQGRTYSEIARVLKPDGYVGLGGSRFLPRTPFSVLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULV2</accession>
    <name>A0A6I8ULV2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4800340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357573.3">
        <property type="nucleotide sequence ID" value="XM_001357536.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULV2"/>
    <dbReference type="KEGG" id="dpo:4800340"/>
    <dbReference type="InParanoid" id="A0A6I8ULV2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026337116" evidence="1">
        <location>
            <begin position="20"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357573.3"/>
        </source>
    </evidence>
    <sequence length="141" mass="16051" checksum="66545406CEC6DE61" modified="2020-08-12" version="1">MYRSCFWLLLVSMLAVVGAGTQSREYLPRRQPQPYFYSTPAPSTMAALRRIIQGHLQRFQLADSPQFSRRALNQQAKTEVGSKMQLLVYVRMPAASEHILDYALPPNTPTPKNYAQNFLPDGKDVVPGSSYIPLRLLLIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMB2</accession>
    <name>A0A6I8UMB2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4800407</name>
        <name evidence="9 10 11" type="synonym">Bet5</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
            <strain evidence="8 9">MV2-25</strain>
            <tissue evidence="9 10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the multisubunit transport protein particle (TRAPP) complex. The heterodimer TRAPPC6B-TRAPPC3 interacts with TRAPPC1 likely providing a core for TRAPP complex formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAPP small subunits family. BET5 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357689.1">
        <property type="nucleotide sequence ID" value="XM_001357652.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038512.1">
        <property type="nucleotide sequence ID" value="XM_015183026.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038513.1">
        <property type="nucleotide sequence ID" value="XM_015183027.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMB2"/>
    <dbReference type="SMR" id="A0A6I8UMB2"/>
    <dbReference type="GeneID" id="4800407"/>
    <dbReference type="KEGG" id="dpo:4800407"/>
    <dbReference type="OMA" id="GKLMYGM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072429">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14855">
        <property type="entry name" value="TRAPPC1_MUM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000004">
        <property type="entry name" value="Trafficking protein particle complex 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF16">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001357689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015038512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015038513.1"/>
        </source>
    </evidence>
    <sequence length="145" mass="16979" checksum="C1DF70D50432BEB1" modified="2020-08-12" version="1">MTIFNLYIFDKCGTLMHYAEWNRTKKSGITREEEAKLTYGMLFSIKSFVSKISPYDPREGFLYYKTNRYALHYLETPSGLKFVLNTDTTAINVKELLQQLYAKVWVEYVVRDPLWTPGAVVTSELFHSKLDEFIKQSPIFGIRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNJ2</accession>
    <name>A0A6I8UNJ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4801252</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358375.4">
        <property type="nucleotide sequence ID" value="XM_001358338.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNJ2"/>
    <dbReference type="FunCoup" id="A0A6I8UNJ2">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801252"/>
    <dbReference type="InParanoid" id="A0A6I8UNJ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026245918" evidence="3">
        <location>
            <begin position="20"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358375.4"/>
        </source>
    </evidence>
    <sequence length="798" mass="90713" checksum="CFB89B31AD510607" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNR6</accession>
    <name>A0A6I8UNR6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein HEXIM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Hexim</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HEXIM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358468.2">
        <property type="nucleotide sequence ID" value="XM_001358431.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNR6"/>
    <dbReference type="FunCoup" id="A0A6I8UNR6">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="GeneID" id="4801361"/>
    <dbReference type="KEGG" id="dpo:4801361"/>
    <dbReference type="InParanoid" id="A0A6I8UNR6"/>
    <dbReference type="OMA" id="KNQYPPW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077501">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097322">
        <property type="term" value="F:7SK snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024872">
        <property type="entry name" value="HEXIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13469">
        <property type="entry name" value="HEXAMETHYLENE BISACETAMIDE INDUCIBLE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13469:SF8">
        <property type="entry name" value="HEXIM P-TEFB COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15313">
        <property type="entry name" value="HEXIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02094">
        <property type="entry name" value="HEXIMFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="216"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="144"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358468.2"/>
        </source>
    </evidence>
    <sequence length="341" mass="36971" checksum="5978FF407053FAB5" modified="2020-08-12" version="1">MAEAVKTESKSQRPLDGGGGGGGSGSGGPAIGNHNSMPKRKHRRGKKSKMVPKKTKNQYPQWKLDMASGQGSTATTRTKLLRSRSLLVPYNTNRFLMEEHMSEVPKDDSDDNCFGSQTEVLFLSKEFSNVYERVRIERLETMSKAELIQECLQIEDRYSKAQNISKEFGAKIRALDEKMRQLTRENQFLRSHFLRSCSAAAGPAAAAAAAASPPSAVAPTASSACELQARQQPAQQQPTPMDSSSEDSESDSSSTTSSTTSSTTSNSSDAHEMGVAGLNIANGHAERSIHEGRGSRSRSRSPIHLNGHATEEERLRLLDPNPMDEDGNSSDEPKNGDATDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPI7</accession>
    <name>A0A6I8UPI7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cysteine and histidine-rich domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mora</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358823.2">
        <property type="nucleotide sequence ID" value="XM_001358786.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPI7"/>
    <dbReference type="FunCoup" id="A0A6I8UPI7">
        <property type="interactions" value="2400"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801786"/>
    <dbReference type="InParanoid" id="A0A6I8UPI7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06488">
        <property type="entry name" value="p23_melusin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1130.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007051">
        <property type="entry name" value="CHORD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039790">
        <property type="entry name" value="CHRD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983:SF3">
        <property type="entry name" value="CHPADIPLOID STATE MAINTENANCE PROTEIN CHPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04968">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51401">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CHORD" evidence="6">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CHORD" evidence="6">
        <location>
            <begin position="149"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="5">
        <location>
            <begin position="219"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358823.2"/>
        </source>
    </evidence>
    <sequence length="365" mass="40681" checksum="0573AB927857A2A5" modified="2020-08-12" version="1">MLQCYNRGCGQLFDEAANTDESCRHHPGEPFFHDAYKGWSCCNKKSVDFTEFLNIKGCTLAKHSNVKPPEPEKPPKDNNGDKDEVIEVRAPIKEAMARPPIETPFTVLQPTVAASLKEQIDTLKVSSAPSATATPSVVESDAIAVGTSCKNNGCSYSFTGLDSDDGQCAYHPGVPIFHEGMKFWSCCQKRTSDFTQFMAQRGCVNGKHKWVKENDDKKVVQCRYDWHQTATNVVVAIYAKKYHYAQSVIEINPIRLHVKLVFPEQENATFDIDLELGGIVKVDKASAHMYGTKVEITLPKLEPGSWSKLNFKRAVMPAAKKTNAAQVPKIDDSDDEFFDLDDIEPVYTHGLKLSDMSLQNPNNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPU4</accession>
    <name>A0A6I8UPU4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3'(2'),5'-bisphosphate nucleotidase 1</fullName>
            <ecNumber evidence="15">3.1.3.57</ecNumber>
            <ecNumber evidence="3">3.1.3.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">3'-phosphoadenosine 5'-phosphate phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Bisphosphate 3'-nucleotidase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Inositol-polyphosphate 1-phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC4801965</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1D-myo-inositol 1,3,4-trisphosphate + H2O = 1D-myo-inositol 3,4-bisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:70319"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:83241"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:70320"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol 1,4-bisphosphate + H2O = 1D-myo-inositol 4-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15553"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58282"/>
            <dbReference type="ChEBI" id="CHEBI:58469"/>
            <dbReference type="EC" id="3.1.3.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15554"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.57" evidence="15"/>
    <dbReference type="EC" id="3.1.3.7" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001358979.3">
        <property type="nucleotide sequence ID" value="XM_001358942.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPU4"/>
    <dbReference type="FunCoup" id="A0A6I8UPU4">
        <property type="interactions" value="971"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801965"/>
    <dbReference type="InParanoid" id="A0A6I8UPU4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004441">
        <property type="term" value="F:inositol-1,4-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01640">
        <property type="entry name" value="IPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000006">
        <property type="entry name" value="Bisphosphate nucleotidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000023">
        <property type="entry name" value="Protein CBR-TAG-231"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF5">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0452">Lithium</keyword>
    <keyword evidence="7 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="18">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001358979.3"/>
        </source>
    </evidence>
    <sequence length="305" mass="32937" checksum="2214870D0F140AC4" modified="2020-08-12" version="1">MASTAPVIMRIMASSIQTAKRAGGIIRDVLKQGDLGIVDKGKNDPQTEADRSAQRCIIASLTKKFPGVKIIGEEGGSDLNVCDDWLVEDLDEEFLQKSCPAEWRDAKPEDFVIWVDPLDGTAEYTQGYVEHVTVLIGIAVKDAAVGGIIHQPFYQQPDGELGRTIWGLKGLGTGGFTHSLAPPGEFIITTTRSHSNALHQQALNAFPATKILKVGGAGFKVLQLLEGKAHAYLFATPGCKKWDTCAPEAVLEAQGGFLTDINGKNYSYNADVEHVNRQGVLASVGENHSELVYKIPRKVREAVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URJ0</accession>
    <name>A0A6I8URJ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arrestin domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4802977</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359786.2">
        <property type="nucleotide sequence ID" value="XM_001359749.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URJ0"/>
    <dbReference type="FunCoup" id="A0A6I8URJ0">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802977"/>
    <dbReference type="InParanoid" id="A0A6I8URJ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF167">
        <property type="entry name" value="ARRESTIN C-TERMINAL-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="4">
        <location>
            <begin position="176"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="317"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360713.2">
        <property type="nucleotide sequence ID" value="XM_001360676.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTJ9"/>
    <dbReference type="FunCoup" id="A0A6I8UTJ9">
        <property type="interactions" value="1712"/>
    </dbReference>
    <dbReference type="GeneID" id="4804095"/>
    <dbReference type="KEGG" id="dpo:4804095"/>
    <dbReference type="InParanoid" id="A0A6I8UTJ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081417">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039340">
        <property type="entry name" value="Tfc4/TFIIIC-102/Sfc4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="439"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="796"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001360713.2"/>
        </source>
    </evidence>
    <sequence length="870" mass="99590" checksum="7A767A0AC7F69AF2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTV8</accession>
    <name>A0A6I8UTV8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4803915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360558.2">
        <property type="nucleotide sequence ID" value="XM_001360521.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTV8"/>
    <dbReference type="FunCoup" id="A0A6I8UTV8">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="GeneID" id="4803915"/>
    <dbReference type="KEGG" id="dpo:4803915"/>
    <dbReference type="InParanoid" id="A0A6I8UTV8"/>
    <dbReference type="OMA" id="NILFDFC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001360558.2"/>
        </source>
    </evidence>
    <sequence length="171" mass="20308" checksum="DEBD4A2F82133C9F" modified="2020-08-12" version="1">MGSSHSRHFLQATRLLKFTNVKCQDLPTTAGFTRYEYCRLKVVRRNHVELSLKVSLLQLPVRNLTTRLQCFQRRDGYRPFMYNVLFDFCKLMAATSRELSFERFIFDTIRKHSNFNHSCPWKENHMTVDKFGLDPTKINVPFPAGVYRLDFTFYAYGIPRTLTQVFFEKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU86</accession>
    <name>A0A6I8UU86_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360968.2">
        <property type="nucleotide sequence ID" value="XM_001360931.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU86"/>
    <dbReference type="GeneID" id="4804398"/>
    <dbReference type="KEGG" id="dpo:4804398"/>
    <dbReference type="InParanoid" id="A0A6I8UU86"/>
    <dbReference type="OMA" id="GEHFINY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073901">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39951">
        <property type="entry name" value="FI22632P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39951:SF2">
        <property type="entry name" value="IP05660P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026125329" evidence="1">
        <location>
            <begin position="26"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360968.2"/>
        </source>
    </evidence>
    <sequence length="116" mass="13147" checksum="2AACD2F63E319EC0" modified="2020-08-12" version="1">MLHLGFVYLLTLLLVLLQISQPADGIIFRLISETLQNNVAGEPITHQVTQWNFDPDAAKKARSLYYEKNGYRSSKFIERLGAGVDGLHEERRLQQAIRDMGRLGGEHNENYPPSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUA0</accession>
    <name>A0A6I8UUA0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 21 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RabX1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361028.1">
        <property type="nucleotide sequence ID" value="XM_001360991.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUA0"/>
    <dbReference type="FunCoup" id="A0A6I8UUA0">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="GeneID" id="4804470"/>
    <dbReference type="KEGG" id="dpo:4804470"/>
    <dbReference type="InParanoid" id="A0A6I8UUA0"/>
    <dbReference type="OMA" id="KWVNEVE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077750">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001769">
        <property type="entry name" value="ras-related protein RABF2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361028.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29138" checksum="4DB0CB46305A75A9" modified="2020-08-12" version="1">MRGIEGKVVVLGSRGVGKTRLVIRYIKNTLHRSESEVPTIAVSFFTCNIILDEVKIKLQIWDTAGQERYRAVAPMYYRNANAAILVFDLTQYKTFTEIKSWIQELHRNVQDPMILTLVGNKMDMQAQRAVSRDEAFLFATSIGATYFETSTETDQGLEQVFLSTALGMVRLADEGKSHSLRSFESTDSLAYTNTNTAFSHTVAALARSPGNAAFRLPYVDIGLAVEGEDVKTNGVGRLETPPWSIEHIALGEVERPSWCCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUN6</accession>
    <name>A0A6I8UUN6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Testicular haploid expressed gene protein-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361160.3">
        <property type="nucleotide sequence ID" value="XM_001361123.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUN6"/>
    <dbReference type="FunCoup" id="A0A6I8UUN6">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804628"/>
    <dbReference type="InParanoid" id="A0A6I8UUN6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="590"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361160.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="635" mass="71818" checksum="F494CDB087E38E5B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUZ9</accession>
    <name>A0A6I8UUZ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein ETH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ETH</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361212.2">
        <property type="nucleotide sequence ID" value="XM_001361175.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUZ9"/>
    <dbReference type="FunCoup" id="A0A6I8UUZ9">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804693"/>
    <dbReference type="InParanoid" id="A0A6I8UUZ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026255778" evidence="1">
        <location>
            <begin position="22"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361212.2"/>
        </source>
    </evidence>
    <sequence length="207" mass="23593" checksum="F8CB750A52596773" modified="2020-08-12" version="1">MRSLTVLGVILLLVLVATSQADDSPGFFLKITKNVPRLGKRSESFGMKNLKTIPRIGRSEQQTAVTPLLSWLWDVDVSQPSKRRLATGEAAGQERELTVVQPVNANTLMELLDNNAIPSEHVKFVHWKDFDRALQSDTDLYAKVIQLGRRPDHRLKEDLNFNSYVPIFDGNGDQNAPFMMYNNNNEDRDLYGGGNRYDRNFLKYNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVM0</accession>
    <name>A0A6I8UVM0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4805133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361587.2">
        <property type="nucleotide sequence ID" value="XM_001361550.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVM0"/>
    <dbReference type="FunCoup" id="A0A6I8UVM0">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="4805133"/>
    <dbReference type="KEGG" id="dpo:4805133"/>
    <dbReference type="InParanoid" id="A0A6I8UVM0"/>
    <dbReference type="OMA" id="GGLANNW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074234">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001361587.2"/>
        </source>
    </evidence>
    <sequence length="131" mass="15196" checksum="7F915E9D97232E6E" modified="2020-08-12" version="1">MEVDEGKALVIRMTQKNKETQTSTAKPGPINWLPQVAAYPTVSPRHTGQPLGPIQAFMPRQSSSSSQFYMHREQELYRRACYLKGLRLGCLKLREHFLTGPSDEEELQPSYRYTLEEMKSLNPYRLDNYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVM6</accession>
    <name>A0A6I8UVM6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4804841</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361337.4">
        <property type="nucleotide sequence ID" value="XM_001361300.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVM6"/>
    <dbReference type="FunCoup" id="A0A6I8UVM6">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804841"/>
    <dbReference type="InParanoid" id="A0A6I8UVM6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361337.4"/>
        </source>
    </evidence>
    <sequence length="439" mass="50250" checksum="DD4801749AD55560" modified="2020-08-12" version="1">MNFPCPATQSRHGRTYKRLQSSLKHRHSTGGRMVLRKVFPAMFTLPKEAPACSRNGTLYLLRCIFLMGVRRPPARFFVAYCLWSFALNLCSTFYQPIGFLTGYISHLSEFSPGEFLTSLQVAFNAWSCSTKVLIVWALVKHFDDANDILDEMDRRLTQPSERLRVHQAVSRSNRIFFIFMTVYMVYATNTFLTAILMGRPPYQNYYPYLDWRSSSWQLGLQSGLEYFAMAGACFQDVCVDCYPVNYVLVLRAHMSIFADRLRQLGSDPEESPEQRYEQLIQCIQDHKTILRFVDCLRPVISGTIFVQFLVVGLVLGFTLINIVLFANLGSAIAALSFMAAVLLETTPFCILCNYLTDDCYNLADALFESNWIDGEQRYKKTLMYFLQKLQQPITFMAMNVFPISVGTNISVTKFSFSVFTLVKQMNISEKLSKVEGEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVZ4</accession>
    <name>A0A6I8UVZ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G protein alpha o subunit isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Galphao</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-alpha family. G(i/o/t/z) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361755.1">
        <property type="nucleotide sequence ID" value="XM_001361718.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVZ4"/>
    <dbReference type="SMR" id="A0A6I8UVZ4"/>
    <dbReference type="FunCoup" id="A0A6I8UVZ4">
        <property type="interactions" value="496"/>
    </dbReference>
    <dbReference type="GeneID" id="4805339"/>
    <dbReference type="KEGG" id="dpo:4805339"/>
    <dbReference type="InParanoid" id="A0A6I8UVZ4"/>
    <dbReference type="OMA" id="GKKDYMP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075321">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
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        <location>
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            <end position="48"/>
        </location>
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            <name>GTP</name>
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            <dbReference type="RefSeq" id="XP_001361755.1"/>
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    <sequence length="354" mass="40580" checksum="21AFB1129511599E" modified="2020-08-12" version="1">MGCTTSAEERAAIQRSKQIEKNLKEDGIQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTAEDFKQYRPVVYSNTIQSLVAILRAMPNLSIQYSNNERESDAKMVFDVCQRMHDTEPFSEELLAAMKRLWQDTGVQECFSRSNEYQLNDSAKYFLDDLDRLGAKDYQPTEQDILRTRVKTTGIVEVHFSFKNLNFKLFDVGGQRSERKKWIHCFEDVTAIIFCVAMSEYDQVLHEDETTNRMQESLKLFDSICNNKWFTDTSIILFLNKKDLFEEKIRKSPLTICFPEYTGGQEYGEAAAYIQAQFEAKNKSTSKEIYCHMTCATDTNNIQFVFDAVTDVIIANNLRGCGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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    <name>A0A6I8UW23_DROPS</name>
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        <submittedName>
            <fullName evidence="12">Regulator of G-protein signaling 17</fullName>
        </submittedName>
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        <name evidence="12" type="primary">Dhit</name>
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    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
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        <subcellularLocation>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="DOUBLE HIT, ISOFORM B"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RGS" evidence="10">
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            <begin position="153"/>
            <end position="269"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="14"/>
            <end position="63"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
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            <begin position="31"/>
            <end position="58"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361788.3"/>
        </source>
    </evidence>
    <sequence length="285" mass="30665" checksum="362BF712776AF9B5" modified="2020-08-12" version="1">MSCTISELPSGCRLRGGMTASTPQSAANNGANGGGNGNGNNGNGNGNGNGNGNGGGGSESAVSVSVSVGGGGVTAASATSNVLQESNAATLQRPQSQKPCCFCWCCCCSCSWGKCLAIKNADENAPTKRDLANSEFLDGEQPTLEEIRSWGKSFDKLMKSSAGRKVFQNFLRSEFSEENILFWLAVEDLKKENSAEVVEEKARLIYEDYISILSPREVSLDSRVREIVNRNMIEPTTHTFDEAQIQIYTLMHRDSYPRFLNSQKFKTLAQMQDNSNAGSKAESPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWC3</accession>
    <name>A0A6I8UWC3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KH domain-containing, RNA-binding, signal transduction-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">qkr58E-3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361645.2">
        <property type="nucleotide sequence ID" value="XM_001361608.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWC3"/>
    <dbReference type="FunCoup" id="A0A6I8UWC3">
        <property type="interactions" value="1683"/>
    </dbReference>
    <dbReference type="GeneID" id="4805200"/>
    <dbReference type="KEGG" id="dpo:4805200"/>
    <dbReference type="InParanoid" id="A0A6I8UWC3"/>
    <dbReference type="OMA" id="EYDHRMA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077548">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22384">
        <property type="entry name" value="KH-I_KHDRBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000052">
        <property type="entry name" value="Kep1, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF140">
        <property type="entry name" value="GH05812P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="81"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="262"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001361645.2"/>
        </source>
    </evidence>
    <sequence length="325" mass="37410" checksum="6E5770A7034EB0DC" modified="2020-08-12" version="1">MEGSGEFIEKFIEKPPVHDHQPRLNEVAQKFLADLDEERQRLSAEFPLCALLIDEAVDRVYCTGRIPGKEFYADVYKQKPMKITQKVFVPVKQYPKFNFTGKILGPKGNSLRRLQEETQCKIAIKGRSSIRDRNKEEQLRNSGDPRYSHLQKDLFLEVSTVATPAECYARIAYALAEIRKYLIPDKNDEVSHEQLRELMEMDPESAKSIHGPNLEAYRSVFDKKFGAGSSSAPKYINLIKRAAENPPEAEEAEEAVYAYEHPHRMPPKRGPPTGYEYSKPRPSIIPTNAAAYKRPQPYPTDMKRMREPPIKSYKPNTYTILKKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWG7</accession>
    <name>A0A6I8UWG7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transmembrane emp24 domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4805267</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361692.2">
        <property type="nucleotide sequence ID" value="XM_001361655.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWG7"/>
    <dbReference type="SMR" id="A0A6I8UWG7"/>
    <dbReference type="FunCoup" id="A0A6I8UWG7">
        <property type="interactions" value="374"/>
    </dbReference>
    <dbReference type="GeneID" id="4805267"/>
    <dbReference type="KEGG" id="dpo:4805267"/>
    <dbReference type="InParanoid" id="A0A6I8UWG7"/>
    <dbReference type="OMA" id="EYMHFRY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081673">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026119849" evidence="11">
        <location>
            <begin position="19"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="12">
        <location>
            <begin position="28"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001361692.2"/>
        </source>
    </evidence>
    <sequence length="204" mass="23369" checksum="F12CD95A504D96EF" modified="2020-08-12" version="1">MFPAICILFALLLESAGGFLITVDAHEEICFHEHVLENERITISFEVMEGGFKDIGVSIKGPEAELLHHSDRDSVGSFTFSASKRGVYTLCFDNQLSTLTPKVLMFQFHVIRGLAYYTDPQRRGDDVLEQAIVQLMINELSGKMIAVKQEQEYMHVRYRGHLAVSDSVHFRIVSWSIFGPSMLLIMTVLEVYYLKRFFEVRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWZ1</accession>
    <name>A0A6I8UWZ1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transcription factor grauzone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">grau</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361903.2">
        <property type="nucleotide sequence ID" value="XM_001361866.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWZ1"/>
    <dbReference type="FunCoup" id="A0A6I8UWZ1">
        <property type="interactions" value="2260"/>
    </dbReference>
    <dbReference type="GeneID" id="4805512"/>
    <dbReference type="KEGG" id="dpo:4805512"/>
    <dbReference type="InParanoid" id="A0A6I8UWZ1"/>
    <dbReference type="OMA" id="WFEVSPN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077321">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039970">
        <property type="entry name" value="TF_Grauzone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23225:SF2">
        <property type="entry name" value="AT09679P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23225">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
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            <end position="80"/>
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        <location>
            <position position="4"/>
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            <position position="7"/>
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    <sequence length="585" mass="67402" checksum="C2FF03C25AF67B0F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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            <dbReference type="RefSeq" id="XP_002132652.2"/>
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    <sequence length="694" mass="79036" checksum="467EB2A629D1E713" modified="2020-08-12" version="1"> </sequence>
</entry>
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</entry>
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        <source>
            <dbReference type="RefSeq" id="XP_002133157.2"/>
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    <sequence length="372" mass="42481" checksum="DBDDAC4FCF43B5E7" modified="2020-08-12" version="1">MNSNAKKQLTEVNVLGDRFLWDMGRLCMNELYSDVWFLVEDQRIPAHRLILAARSDYFRGLLYGSMCESAETEIRLLEVPLEAFKVILGYLYSGTLPVSTLDLDSIFKVLGVANLYCLLEVESVLADHLKQNVAVDNVCRILETARLYGFSDLAENCFKFMDREASKLVKHDSFQMLSKELLEEVLPRDTFYVLEDELFQFVWKWSRQNPDVDIKSLVPCVRLPLLSAKQLTNAVGLSGLVEPAKAFDTNLAENVPYRYVFVEPGSSVRPERILRSNVWDFGKMFRVNQIKCMLKPPICCHVDVSCDKTHWERVGTNITATIVFNLRFTVLTEQVINFTARPVYYIRFIGSGSGRPIWKCVKMLTAGNSGDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ50</accession>
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        <recommendedName>
            <fullName evidence="11">Malate dehydrogenase</fullName>
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        </recommendedName>
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        <name evidence="15" type="primary">LOC6902317</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 11">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="11"/>
    <dbReference type="RefSeq" id="XP_002133844.2">
        <property type="nucleotide sequence ID" value="XM_002133808.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ50"/>
    <dbReference type="KEGG" id="dpo:6902317"/>
    <dbReference type="InParanoid" id="A0A6I8UZ50"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ50">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000268">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="12">
        <location>
            <begin position="34"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="178"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="39"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002133844.2"/>
        </source>
    </evidence>
    <sequence length="343" mass="36567" checksum="DF5F52286C3B07EB" modified="2020-08-12" version="1">MLGKQFQSSAISQCRVCWRLYQATPGQMQTRQFRVAVIGAVGGIGQPLSLLLMANKLVTELVLHDLTATKGFGQDLSHIDRACQVKSFFGEDQMKSAIKGSDIVMITAGMPRKPGQTRDFLFDTNAPIVAKAAGLVSQHAAHAMVGIITNPVNAVVAVAAEAMKKAGYYDPDRLFGVTTLDVVRAEKFIGDHMSIHPGKVRIPVIGGHAGTTIVPIFSQCQPPFEGDEKCIASIVKRIQTGGDEVVKAKAGQGSATLSMAYAAARFTNALMLGLKGEPGPPECAYVQSDATEAPFFSTPLTLGPKGIEENHGLPELDECEKEQLKVAVDALKKSAAKGVKFME</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ99</accession>
    <name>A0A6I8UZ99_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6901254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133955.3">
        <property type="nucleotide sequence ID" value="XM_002133919.3"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8UZ99">
        <property type="interactions" value="7"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A6I8UZ99"/>
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    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133955.3"/>
        </source>
    </evidence>
    <sequence length="383" mass="44861" checksum="6E259EAED56E59F2" modified="2020-08-12" version="1">MTSILDMKAGPPDYWAEISNFFLPLKYLITNDRFDAFVQDIDLYYLINAVFWIFVALSCGFYGFQRVFQFFLKSSNMKYFKRKQFARLIWNIAFYAASCLFLHFYNEFMILPQLMKNQGRYSLFYSSDNLIFYRSHQLEKFQFYSIFIITFYLHGAMLDYKEADFLEVGSKGLYLLALVAIDVYRYENYFVGLNLTLGLYSILTEVLALLSLPSSNRNRLVYQMFLGLRIAAWAHVFINLLPFKYFIPTLFAKNFKLPLNVAIWLWYGLSIWNSPVLQYFYHQIYHSNPSDCSGEGSAAKCILVKDSIEYRHYKTLKKAYLEVKLAHEKATANAMPASESTSASAKTFQAIKCVMMLKRKLKRIREGRGHEPEDDNEDVTTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZN2</accession>
    <name>A0A6I8UZN2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma</fullName>
            <ecNumber evidence="10">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">sl</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="10"/>
    <dbReference type="RefSeq" id="XP_002134355.2">
        <property type="nucleotide sequence ID" value="XM_002134319.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZN2"/>
    <dbReference type="FunCoup" id="A0A6I8UZN2">
        <property type="interactions" value="1027"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814530"/>
    <dbReference type="InParanoid" id="A0A6I8UZN2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZN2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010634">
        <property type="term" value="P:positive regulation of epithelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16201">
        <property type="entry name" value="EFh_PI-PLCgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13362">
        <property type="entry name" value="PH_PLC_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08592">
        <property type="entry name" value="PI-PLCc_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10341">
        <property type="entry name" value="SH2_N-SH2_PLC_gamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11825">
        <property type="entry name" value="SH3_PLCgamma"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000351">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000199">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000004">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
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    <dbReference type="FunFam" id="3.20.20.190:FF:000043">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000011">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056586">
        <property type="entry name" value="EF-hand_PLCG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016279">
        <property type="entry name" value="PLC-gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035024">
        <property type="entry name" value="PLC-gamma_N-SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057061">
        <property type="entry name" value="PLCG_EF-hand_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF159">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23329">
        <property type="entry name" value="EF_HAND_1_PLCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23583">
        <property type="entry name" value="EF_HAND_2_PLCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000952">
        <property type="entry name" value="PLC-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 10 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10 13" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8 10 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="20"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="603"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="715"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="838"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="18">
        <location>
            <begin position="994"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="17">
        <location>
            <begin position="1106"/>
            <end position="1227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_002134355.2"/>
        </source>
    </evidence>
    <sequence length="1250" mass="143666" checksum="34A969CE0EFF61D8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0V6</accession>
    <name>A0A6I8V0V6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">pH-sensitive chloride channel 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Ligand-gated chloride channel protein hodor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SecCl</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:29824"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 16">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135057.2">
        <property type="nucleotide sequence ID" value="XM_002135021.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0V6"/>
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        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900693"/>
    <dbReference type="InParanoid" id="A0A6I8V0V6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099095">
        <property type="term" value="F:ligand-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18987">
        <property type="entry name" value="LGIC_ECD_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000042">
        <property type="entry name" value="Blast:Glycine receptor subunit alpha-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 16" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026378446" description="pH-sensitive chloride channel 2" evidence="16">
        <location>
            <begin position="27"/>
            <end position="511"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
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        <location>
            <begin position="49"/>
            <end position="270"/>
        </location>
    </feature>
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        <location>
            <begin position="279"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_002135057.2"/>
        </source>
    </evidence>
    <sequence length="511" mass="59367" checksum="4511978E55C2B769" modified="2020-08-12" version="1">MNHSRNVLWTTLIFGSILVLFQASIGATIDTVEDQDCPSLANASYMQHSQLIQTLTHVCRYDRLERPIEYDSDAKRLPIVVKTRIYVYFLQNLNSDLLQFKMHALLQLRFQDKRLAYKAFQRDDNILGQKHLSERLWLPHIFFANERESSILGTDEKDVLTSLSPEGNVILSTRMQASLYCWMNFKKFPFDQQLCSTVIESWMYNTSDLVLEWEERSPISFDPEMRLTEYNMEKFWHNTTIVHSDEINLRHGAFLGDYSSLSFTVNLRREIGFYLLDYYLPSMMIVAISWVSFWLQADASPPRIMLGTSTMLSFITLSSSQSKNLPKVSYIKVSEVWFLGCTFFIFGSLVEFAFVNTIWRRKENIELKKVNSKYIIKSTLTPRPSRRQMGGSLSNDSRARSCSSLDNIVSSTESVRNGNGNVNHGYNNYLTVHPNLPIIKTECADNVSISSERMSNEHIIDIDKEKKETPPTFTTMTPQEIAMWIDRRSRFLFPVMFLAFNALYWTFVYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1A6</accession>
    <name>A0A6I8V1A6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135495.2">
        <property type="nucleotide sequence ID" value="XM_002135459.3"/>
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    <dbReference type="KEGG" id="dpo:6900156"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
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    <dbReference type="ExpressionAtlas" id="A0A6I8V1A6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026137693" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135495.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20014" checksum="D113864AC5A17E21" modified="2020-08-12" version="1">MMQLIHMAMGWVLLSSALQAAILPLTLIEKGSVDDKPFPLDTEKFGYLELKPNGSLILRRGPGQSGSNRQNLLLLRGVLQAIKQDNHHSHGGSELNIRIYGDGVEHKFPPLLENIIQRIQTYFSVYRYTDTSQPVRRDEAPTKEAIDGDLGVTTVRTGGDEDDLIAIGEQDAYITVGEDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1U6</accession>
    <name>A0A6I8V1U6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein Ir94h</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ir94h</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <dbReference type="FunCoup" id="A0A6I8V1U6">
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
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        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="531"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002137006.2"/>
        </source>
    </evidence>
    <sequence length="563" mass="64608" checksum="3D889E07D1421A57" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2Q1</accession>
    <name>A0A6I8V2Q1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Axoneme-associated protein mst101(2)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137436.2">
        <property type="nucleotide sequence ID" value="XM_002137400.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2Q1"/>
    <dbReference type="KEGG" id="dpo:6897276"/>
    <dbReference type="InParanoid" id="A0A6I8V2Q1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007999">
        <property type="entry name" value="DUF745"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37161:SF2">
        <property type="entry name" value="AT11648P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37161">
        <property type="entry name" value="HDC10475"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05335">
        <property type="entry name" value="DUF745"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026003558" description="Axoneme-associated protein mst101(2)" evidence="2">
        <location>
            <begin position="23"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="29784" checksum="3E39438AB5BED380" modified="2020-08-12" version="1">MHYHKVGITLLVLLYGISGLESIYLYDDSPQLDESDGRQFSNLQMDSVSGEGGCGGVSVSSKAKQCGNFDKGNPKSKASNIAQKAAQDAKAASDAQMSAAEAAATQVKTELAEKAAQSARAAEAALAGKQEMVEQLEQEMAEAEAVVNDVTNSLQNTQANSNAAAAMATESQSQVESLKRLLAAATANLVNIEGVSQGAQTELSEKTQLLEAAKNRVQNLGKQIADAKKDFDATKQAAYKAACAAVEAKKKASQRMRRMAAYRQWIMEQPRKYVRGGAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2V6</accession>
    <name>A0A6I8V2V6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Facilitated trehalose transporter Tret1-2 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pippin</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002136817.3">
        <property type="nucleotide sequence ID" value="XM_002136781.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8V2V6"/>
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        <location>
            <begin position="59"/>
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            <begin position="132"/>
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            <begin position="466"/>
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            <begin position="59"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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            <dbReference type="RefSeq" id="XP_002136817.3"/>
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    <sequence length="533" mass="59055" checksum="BF08E618D19FB154" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3E8</accession>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <strain evidence="7">MV-25-SWS-2005</strain>
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</entry>
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    <accession>A0A6I8V3I9</accession>
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        <name evidence="2" type="primary">LOC6897680</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002137815.4"/>
        </source>
    </evidence>
    <sequence length="297" mass="34110" checksum="6D4E5C7132C02D26" modified="2020-08-12" version="1">MIEEKKSDLPELSVPGFKYEQLREVLLRMRALQRYSFCVEAQIDSNLMQLRQGFLRLLEDYLTLEEVASLYTVSVLMFLVTENERPRQFDERLELLNASFNYSDTLKPTMIHYATITMLDSLKLTPCEEEHGYIYMLEAMPHILVACNTGKMIALGLLRAFIGALSNTTLVLHIYRNLWEVYDMVQSISQDTILADWSLCKTYILVKSFALLMSSTAFNSCNLGLRQAGYQGFQMPPIAAEISEWFTALRDKIEEKHTNLSSKSGFKLVRFIDQNILRYLTEESNETTSEAGIETVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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    <name>A0A6I8V3K9_DROPS</name>
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        <submittedName>
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        </submittedName>
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        <name evidence="4" type="primary">RYa</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026779163" evidence="2">
        <location>
            <begin position="34"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138108.2"/>
        </source>
    </evidence>
    <sequence length="193" mass="21091" checksum="7F236D2182B1FDC2" modified="2020-10-07" version="1">MWNANQLPTQPRQFYLGLLLITICAVLSEDVCAVPTDQSYVENTHGQDGGGMQALQKRPAFFVGSRYGRSSGNAVTGGGATSVGSSKTRRLIIVPRNDRFFLGSRYGKRNGEYLSPYEQNSLSLALSKDSSQDVEQQTLNTNSRLRPSLMSCVYTGISNYYRCKGSDHHSAVDESLDSTSMAESDGDTNNVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3T8</accession>
    <name>A0A6I8V3T8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6898062</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138148.3">
        <property type="nucleotide sequence ID" value="XM_002138112.3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898062"/>
    <dbReference type="InParanoid" id="A0A6I8V3T8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23107:SF32">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23107">
        <property type="entry name" value="SYNOVIAL SARCOMA ASSOCIATED SS18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026297279" evidence="3">
        <location>
            <begin position="24"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="510"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="613"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="672"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="958"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1085"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="776"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="672"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="958"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="975"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1085"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1102"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002138148.3"/>
        </source>
    </evidence>
    <sequence length="1372" mass="155993" checksum="4681FD737A25B08F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V419</accession>
    <name>A0A6I8V419_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138390.2">
        <property type="nucleotide sequence ID" value="XM_002138354.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V419"/>
    <dbReference type="GeneID" id="6898336"/>
    <dbReference type="KEGG" id="dpo:6898336"/>
    <dbReference type="InParanoid" id="A0A6I8V419"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246970">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="1">
        <location>
            <begin position="68"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138390.2"/>
        </source>
    </evidence>
    <sequence length="192" mass="22627" checksum="D558A3290DF3FF8A" modified="2020-08-12" version="1">MAFATRKKEDPLSSNDDEILQIKIIELQDKLDDILSNQDLIITLLSQVNPLPNINGPLTEPTEIRTEYFPVREQQELEKLDSELANPGNSFIPMMKRILRPEGIIQPLKDSIPKIFGEEIIMGFNFDGVNHKQPFRQFKNINRTIYEIQKRSGYTEADYIADIRITFHTLKRRYHKRKHDVKRRSKLNKFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5J0</accession>
    <name>A0A6I8V5J0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC13036500</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736517.3">
        <property type="nucleotide sequence ID" value="XM_003736469.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5J0"/>
    <dbReference type="KEGG" id="dpo:13036500"/>
    <dbReference type="InParanoid" id="A0A6I8V5J0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000502">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF0">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="8">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="130"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_003736517.3"/>
        </source>
    </evidence>
    <sequence length="463" mass="51733" checksum="B2EE366F8105F02D" modified="2020-08-12" version="1">MEMSLVRMVRNSKFRHVFGQAQKREDGFENIRVSKTSWDSTLCCANPKFLAIILETSGGGAFIVLPLSQTGRIPSDYPLVSGHTSPVLDIAWCPHDDNLIASASEDCSVKLWMIPDEGLTTTLTQPTVDLVYHERRVALLLWHPSAHNVLLTAGWDKKVVIWNVGTGEILVHLDSHPEVLYSACFNWDGTMLVTTCRDKKIRIFDPRTAKLYSEAICHDSARASRAIFLRSGLVFTTGFNRASDRQYSLRDPQDLCNSIAQGNLDMASGVLFPIYDADTNMIYLCGKGDLVIKFFEVTPEPPYVHYISCFKTTEPQRGIAMMPKRGCDIIACEVARFYRLTSNGQCQVVSMTVPRKSDHFQEDLYPNTLAATAAITAEEWIAGMDAEPVTFSLKDSFIDVTATNQNSNERRPRKRAIGATACRASGSTISRDRVSRAATMWEKLLNKNKAKQMEKQNAEDARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5S0</accession>
    <name>A0A6I8V5S0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prisilkin-39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138634.2">
        <property type="nucleotide sequence ID" value="XM_002138598.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5S0"/>
    <dbReference type="KEGG" id="dpo:6898629"/>
    <dbReference type="InParanoid" id="A0A6I8V5S0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026282516" evidence="1">
        <location>
            <begin position="20"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138634.2"/>
        </source>
    </evidence>
    <sequence length="257" mass="25431" checksum="BE46048B5A8D6E41" modified="2020-08-12" version="1">MNLHARLALMSLLLTAVLAQRSGKSRSDRPQGRTLGLLTPLLLGIGGGSLFDVPVAPVPPQRPVGGGAAAAGAQSDQYYGSSYPSYNYRPSYNPYAYTSSYPGYGYGYGYPGLGLGYGSNYYRPSYNFGGYQRPQQYTGNYYGYRPNYSGGGAVAAGYPSYGSSAAGVSATPAYTPSGSALGVAAGSGVGAGAGVGAGVGSPQLSTEQTAQLAGLLGQALGSSLRPYLANGGGAGGAAAAGGGGNSQALGGLLGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5Z1</accession>
    <name>A0A6I8V5Z1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein groucho isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gro</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat Groucho/TLE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736689.1">
        <property type="nucleotide sequence ID" value="XM_003736641.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5Z1"/>
    <dbReference type="GeneID" id="4802203"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081023">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V5Z1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000001">
        <property type="entry name" value="transducin-like enhancer protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005617">
        <property type="entry name" value="Groucho/TLE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009146">
        <property type="entry name" value="Groucho_enhance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814:SF21">
        <property type="entry name" value="PROTEIN GROUCHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03920">
        <property type="entry name" value="TLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01850">
        <property type="entry name" value="GROUCHOFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Groucho/TLE N-terminal Q-rich" evidence="9">
        <location>
            <begin position="20"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="519"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="561"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="643"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="187"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_003736689.1"/>
        </source>
    </evidence>
    <sequence length="719" mass="78915" checksum="371A5FCEC9565AD0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDP7</accession>
    <name>A0A6I8VDP7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cation-independent mannose-6-phosphate receptor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Lerp</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015038623.2">
        <property type="nucleotide sequence ID" value="XM_015183137.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDP7"/>
    <dbReference type="FunCoup" id="A0A6I8VDP7">
        <property type="interactions" value="247"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VDP7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005520">
        <property type="term" value="F:insulin-like growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007041">
        <property type="term" value="P:lysosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.130.10:FF:000026">
        <property type="entry name" value="cation-independent mannose-6-phosphate receptor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000479">
        <property type="entry name" value="CIMR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF17">
        <property type="entry name" value="CATION-INDEPENDENT MANNOSE-6-PHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00878">
        <property type="entry name" value="CIMR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01404">
        <property type="entry name" value="CIMR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026298840" evidence="10">
        <location>
            <begin position="44"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="863"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="11">
        <location>
            <begin position="51"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="11">
        <location>
            <begin position="200"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="11">
        <location>
            <begin position="347"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="11">
        <location>
            <begin position="504"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="799"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="803"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015038623.2"/>
        </source>
    </evidence>
    <sequence length="931" mass="100641" checksum="6256B383E3BE72EE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VF95</accession>
    <name>A0A6I8VF95_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4804575</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040028.2">
        <property type="nucleotide sequence ID" value="XM_015184542.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040029.2">
        <property type="nucleotide sequence ID" value="XM_015184543.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VF95"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015040028.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015040029.2"/>
        </source>
    </evidence>
    <sequence length="577" mass="63848" checksum="F2BCA8E05210B85D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFP7</accession>
    <name>A0A6I8VFP7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 10">Heterogeneous nuclear ribonucleoprotein L isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10 11" type="primary">sm</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 10">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="8 10">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="9 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 11">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="9 11">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040318.1">
        <property type="nucleotide sequence ID" value="XM_015184832.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040321.1">
        <property type="nucleotide sequence ID" value="XM_015184835.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234539.1">
        <property type="nucleotide sequence ID" value="XM_033378648.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234540.1">
        <property type="nucleotide sequence ID" value="XM_033378649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFP7"/>
    <dbReference type="GeneID" id="4804945"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0264769">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VFP7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12694">
        <property type="entry name" value="RRM2_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12424">
        <property type="entry name" value="RRM3_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12427">
        <property type="entry name" value="RRM4_hnRNPL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000072">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein L isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000052">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein L like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000206">
        <property type="entry name" value="Smooth, isoform L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006536">
        <property type="entry name" value="HnRNP-L/PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055204">
        <property type="entry name" value="HNRNPL_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021790">
        <property type="entry name" value="PTBP1-like_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01649">
        <property type="entry name" value="hnRNP-L_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11835">
        <property type="entry name" value="RRM_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 9 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="80"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="285"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015040318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015040321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033234539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033234540.1"/>
        </source>
    </evidence>
    <sequence length="519" mass="56923" checksum="50BE660C776A82EF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VG88</accession>
    <name>A0A6I8VG88_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cyclic nucleotide-gated cation channel beta-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CngB</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015040505.2">
        <property type="nucleotide sequence ID" value="XM_015185019.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VG88"/>
    <dbReference type="FunCoup" id="A0A6I8VG88">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898825"/>
    <dbReference type="InParanoid" id="A0A6I8VG88"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000149">
        <property type="entry name" value="Cyclic nucleotide-gated cation channel beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000078">
        <property type="entry name" value="Cyclic nucleotide-gated channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF1">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL SUBUNIT B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81324">
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="17 18 19">MV-25-SWS-2005</strain>
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        <source>
            <dbReference type="RefSeq" id="XP_015041398.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015041403.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033241406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033241407.1"/>
        </source>
    </evidence>
    <sequence length="2309" mass="255637" checksum="A5A2D21616DDF4B4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJC9</accession>
    <name>A0A6I8VJC9_DROPS</name>
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        <submittedName>
            <fullName evidence="9 10 11">Cationic amino acid transporter 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">LOC4813539</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
            <tissue evidence="9 10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353160.3">
        <property type="nucleotide sequence ID" value="XM_001353124.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042572.2">
        <property type="nucleotide sequence ID" value="XM_015187086.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238507.1">
        <property type="nucleotide sequence ID" value="XM_033382616.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8VJC9"/>
    <dbReference type="KEGG" id="dpo:4813539"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097638">
        <property type="term" value="P:L-arginine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000010">
        <property type="entry name" value="probable cationic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF105">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="7">
        <location>
            <begin position="537"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="821"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="855"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="921"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1008"/>
            <end position="1075"/>
        </location>
    </feature>
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        <location>
            <begin position="823"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="870"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="891"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="942"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353160.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015042572.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238507.1"/>
        </source>
    </evidence>
    <sequence length="1075" mass="118047" checksum="2ED9042597BEF702" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VM39</accession>
    <name>A0A6I8VM39_DROPS</name>
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        <submittedName>
            <fullName evidence="12">Uncharacterized protein Fhos isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Fhos</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the formin homology family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_015188658.2"/>
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        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VM39"/>
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        <property type="component" value="Chromosome X"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005856">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0051015">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR016024">
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        <property type="entry name" value="FH3_FHOD1-3-like"/>
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    <dbReference type="PANTHER" id="PTHR45920:SF4">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF18382">
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    </dbReference>
    <dbReference type="SMART" id="SM00498">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
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    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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            <end position="1576"/>
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    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="2491"/>
            <end position="2523"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="976"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="1104"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="1533"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1547"/>
            <end position="1594"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="1670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1750"/>
            <end position="1769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1780"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1843"/>
            <end position="1862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1880"/>
            <end position="1899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1911"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2505"/>
            <end position="2526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="145"/>
            <end position="186"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="336"/>
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        <source>
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    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015044144.2"/>
        </source>
    </evidence>
    <sequence length="2552" mass="281458" checksum="BAE2B86E7E4D0EC7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMR1</accession>
    <name>A0A6I8VMR1_DROPS</name>
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        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
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        <name evidence="12 13" type="primary">puf</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
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    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="13">MV-25-SWS-2005</strain>
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    </reference>
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        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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        <text evidence="2">Belongs to the peptidase C19 family.</text>
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    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033232313.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033232317.1"/>
        </source>
    </evidence>
    <sequence length="3961" mass="446213" checksum="D9EE83C590B8CD8C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNP7</accession>
    <name>A0A6I8VNP7_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
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        <name evidence="14" type="primary">Vps15</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033232493.1">
        <property type="nucleotide sequence ID" value="XM_033376602.1"/>
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        <property type="interactions" value="1278"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800362"/>
    <dbReference type="InParanoid" id="A0A6I8VNP7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13980">
        <property type="entry name" value="STKc_Vps15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Blast:Phosphoinositide 3-kinase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000342">
        <property type="entry name" value="Serine/threonine-protein kinase VPS15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000741">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002161">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="28"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="968"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1231"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1312"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1289"/>
            <end position="1314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033232493.1"/>
        </source>
    </evidence>
    <sequence length="1346" mass="149484" checksum="32415D20ACCC6C89" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQQ2</accession>
    <name>A0A6I8VQQ2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Aminopeptidase</fullName>
            <ecNumber evidence="25">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">LOC117183220</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="31">MV-25-SWS-2005</strain>
            <tissue evidence="31">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.</text>
            <dbReference type="EC" id="3.4.11.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23 25">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="23 25">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 25">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="25"/>
    <dbReference type="RefSeq" id="XP_033233213.1">
        <property type="nucleotide sequence ID" value="XM_033377322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQQ2"/>
    <dbReference type="FunCoup" id="A0A6I8VQQ2">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117183220"/>
    <dbReference type="InParanoid" id="A0A6I8VQQ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VQQ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004230">
        <property type="term" value="F:glutamyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000003">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000016">
        <property type="entry name" value="Glutamyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF276">
        <property type="entry name" value="GLUTAMYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 25 31" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12 25" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 23 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17 25" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9 25" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="26" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13 23 25" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026236472" description="Aminopeptidase" evidence="26">
        <location>
            <begin position="23"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="29">
        <location>
            <begin position="75"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="27">
        <location>
            <begin position="294"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="28">
        <location>
            <begin position="594"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="22">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="24">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_033233213.1"/>
        </source>
    </evidence>
    <sequence length="945" mass="107613" checksum="12F094592C0DB01D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTL0</accession>
    <name>A0A6I8VTL0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234253.1">
        <property type="nucleotide sequence ID" value="XM_033378362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTL0"/>
    <dbReference type="KEGG" id="dpo:117183671"/>
    <dbReference type="InParanoid" id="A0A6I8VTL0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026230522" evidence="1">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234253.1"/>
        </source>
    </evidence>
    <sequence length="182" mass="21627" checksum="1E55158852B0631E" modified="2020-08-12" version="1">MRCQLMICAVLGLFLTFHIKINDAVVFKFTNYVCESYNQSWFVFHNYRLKAVSRNKVLLNMNGTILHPANNISIHGRIFKKANGYKPWLFDFFFDACQYLRKRNQPVFNIIYGLFNQFSNINHSCPYVGPQIVKDFYLRPELLRLPFPTGDYLLALQWYFDKKLQFDTNVSFTFVEDLMKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTR1</accession>
    <name>A0A6I8VTR1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Nose resistant to fluoxetine protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC4804866</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040239.2">
        <property type="nucleotide sequence ID" value="XM_015184753.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234466.1">
        <property type="nucleotide sequence ID" value="XM_033378575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTR1"/>
    <dbReference type="KEGG" id="dpo:4804866"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF15">
        <property type="entry name" value="GH19286P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634559" evidence="2">
        <location>
            <begin position="23"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="555"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="592"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="629"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="660"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="691"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Nose resistant-to-fluoxetine protein N-terminal" evidence="3">
        <location>
            <begin position="87"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015040239.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033234466.1"/>
        </source>
    </evidence>
    <sequence length="736" mass="84025" checksum="EB20CF317BB3C19C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VU77</accession>
    <name>A0A6I8VU77_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26532050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234625.1">
        <property type="nucleotide sequence ID" value="XM_033378734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VU77"/>
    <dbReference type="KEGG" id="dpo:26532050"/>
    <dbReference type="InParanoid" id="A0A6I8VU77"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271037">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033234625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15406" checksum="86880CB1893260B5" modified="2020-08-12" version="1">MELWRMDELCLVKILKCLALPDQLAMLQTFQRCRPLLGRIWRSQLTEIELNLLEVPLHKDDFDFLLTNGRAQLNELRLNYLGRDHFEVLVGHRFPQLRLLEVDLLPPFFLCPRLLHQLGQTMPLALGSNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VV12</accession>
    <name>A0A6I8VV12_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234922.1">
        <property type="nucleotide sequence ID" value="XM_033379031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VV12"/>
    <dbReference type="KEGG" id="dpo:117183772"/>
    <dbReference type="InParanoid" id="A0A6I8VV12"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234922.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="27990" checksum="0EDC9B8CA3AD44F9" modified="2020-08-12" version="1">MQRFSDSKFVGRRGCPQRVNCDNATNFVGASRHFSELRRKIEAEADAIREFASRSGCEFAFIPPREPHMGGLWEAGVKSAKGLLLRAVGSALLTAEELETVLVGIEAVLNSRPLGPLSPDPSDGDALTPGHLLTGGPLIAPPAPRTPDQEGLSCLKRWRLVSSARQMFWQRWSREYVLGLQIRFKWHQEEPNIKEGDLVIVAEDNLPPQQWLLGRVVGTTAGQDGRVRVVELRTSSGATFRRPIHKLALLPMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VX04</accession>
    <name>A0A6I8VX04_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATPase ASNA1 homolog</fullName>
            <ecNumber evidence="8">3.6.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arsenical pump-driving ATPase homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Arsenite-stimulated ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC4804910</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_001361395.1">
        <property type="nucleotide sequence ID" value="XM_001361358.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235104.1">
        <property type="nucleotide sequence ID" value="XM_033379213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VX04"/>
    <dbReference type="SMR" id="A0A6I8VX04"/>
    <dbReference type="GeneID" id="4804910"/>
    <dbReference type="KEGG" id="dpo:4804910"/>
    <dbReference type="OMA" id="MDAPYEF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074069">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VX04">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000235">
        <property type="entry name" value="ATPase ASNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03112">
        <property type="entry name" value="Asna1_Get3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027542">
        <property type="entry name" value="ATPase_ArsA/GET3_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="9">
        <location>
            <begin position="22"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001361395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033235104.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="37505" checksum="F5A5591982C1FEDE" modified="2020-08-12" version="1">MVDNLEPLPASLQNLVEQDSLKWIFVGGKGGVGKTTCSSSLAVQLAKVRESVLIISTDPAHNISDAFDQKFTKVPTKVNGFDNLFAMEIDPNAGLNELPEEYFEGENEALRVSKGVMQEMINALPGIDEAMSYAEVMKLVKGMNFSVVVFDTAPTGHTLRLIAFPQVVEKGLGKLLRLKMKVAPLLTQFASMLGMADVNVDTLSQKLDDMLRVITQVNEQFKNPDQTTFVCVCIAEFFSLYETERLIQELTKCGIDVHNIIVNQLLFLNNSHSACKMCASRYKIQEKYLDQIADLYEDFHVTKLPLLEKEVRGPDSIKAFSENLMTPYDPKAKPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VY72</accession>
    <name>A0A6I8VY72_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable G-protein coupled receptor CG31760</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235479.1">
        <property type="nucleotide sequence ID" value="XM_033379588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VY72"/>
    <dbReference type="KEGG" id="dpo:117183943"/>
    <dbReference type="InParanoid" id="A0A6I8VY72"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235479.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="19751" checksum="548F330E69EC4CF8" modified="2020-08-12" version="1">MQMTGEEQPGPEEAELLRQFRRLFAPILDDSLQLYYKLNETDDTAGDTEHIRIHQTVAQLNEQLTSSEEETMETHVNSPSPPVDAELLPLSSCPSSTSSSSLYAIHTPSPAAHASGLLLLPQCLDSPLLSCSPESRTITEQVQLHRPPQQLIEDENNTSCSLSSNLTDSKTLDGRTPIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W083</accession>
    <name>A0A6I8W083_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hepatoma-derived growth factor-related protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC117184102</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HDGF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236174.1">
        <property type="nucleotide sequence ID" value="XM_033380283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W083"/>
    <dbReference type="KEGG" id="dpo:117184102"/>
    <dbReference type="InParanoid" id="A0A6I8W083"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05834">
        <property type="entry name" value="PWWP_HRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036218">
        <property type="entry name" value="HIVI-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021567">
        <property type="entry name" value="LEDGF_IBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550">
        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550:SF70">
        <property type="entry name" value="JIL-1 ANCHORING AND STABILIZING PROTEIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11467">
        <property type="entry name" value="LEDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140576">
        <property type="entry name" value="HIV integrase-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="5">
        <location>
            <begin position="12"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033236174.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="43406" checksum="0806EC08B757E260" modified="2020-08-12" version="1">MANENTCKSFNIGDLVFAKVKGYIPWPAVVSNEVNSKKQYTVLFYGTRETGYRQIQDLLPYAENKEKYSTKEYMKRAGFRKAMIEINKVAEEKANGPKHVDEETEQQLKTTAAVTSFQEPSRPANNEENNMVLVYLPYGKVFGININHNKPESFENAAAEQSWINESRKEAKVQKEQMLSGQLDPKSLPGRIIVEPSPNETGRKEERKLMKDILKLENALTIERDFFDLCAKIRQCLPLRQAHVTKCLKNLGRLKKLELTKVMLLRHPESVDTLRKLQGYVGNLKKWKMPRSDKIKFEAQAKIIRQEATIIYARFPKLFDSAETKDFWQEYCKEVKIFKETTKKVNSSIRVALDERMYNNLIKAGQENTPAPAETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0Q3</accession>
    <name>A0A6I8W0Q3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">LOC6903865</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15 16">MV-25-SWS-2005</strain>
            <tissue evidence="14 15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236915.1">
        <property type="nucleotide sequence ID" value="XM_033381024.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236916.1">
        <property type="nucleotide sequence ID" value="XM_033381025.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236917.1">
        <property type="nucleotide sequence ID" value="XM_033381026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0Q3"/>
    <dbReference type="KEGG" id="dpo:6903865"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017126">
        <property type="entry name" value="Condensin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033236915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033236916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033236917.1"/>
        </source>
    </evidence>
    <sequence length="721" mass="81302" checksum="4184F1DF3CC8839D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4B2</accession>
    <name>A0A6I8W4B2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Saccharopine dehydrogenase-like oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC26533820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238158.1">
        <property type="nucleotide sequence ID" value="XM_033382267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4B2"/>
    <dbReference type="FunCoup" id="A0A6I8W4B2">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26533820"/>
    <dbReference type="InParanoid" id="A0A6I8W4B2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000178">
        <property type="entry name" value="Saccharopine dehydrogenase-like oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033238158.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="46888" checksum="FB19C07C4C19BEB1" modified="2020-08-12" version="1">MAAKKLDAIIFGATGFTGKYTVLEAVSVLKGLNWGIAGRSQEKLQSVLKEMSAKSKTDLSQTPIIIADVNNEASLIEMAKRCRIVVNTAGPYRFFGENVVKACIEAGAHHVDVSGEPQYMETMQLKYNDRARERGVYVISACGFDSIPADMGVVFIEKNFDGVINSVENFVHLGVKGGTKGLGGAALNTGTWESAIHAIANRSESIAIRRKLFPDRLPKFHPALKSRAPLSIAEDVGKVILPFPETDRSVVMRSQRYLYETEKKRPIQMQAYMTYPSRLVAGVVVFFASIIGIMAQFSFGRQLLLKYPSFFSGGMASRDGPSEARMERSFFSMTMKASGWPKSQKLAESTDQYTSPPSKTLTVKITGPNPGYGSTCVALLSTAKTILNESDKMPSTGGVLPPGAAFSKTSLISELEKHEHGIKYEIVANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W632</accession>
    <name>A0A6I8W632_DROPS</name>
    <protein>
        <submittedName>
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            <taxon>Diptera</taxon>
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            <taxon>Drosophila</taxon>
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    <dbReference type="RefSeq" id="XP_002133455.2">
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        <property type="nucleotide sequence ID" value="XM_033382919.1"/>
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        <property type="nucleotide sequence ID" value="XM_033382920.1"/>
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        <property type="entry name" value="AT HOOK TRANSCRIPTION FACTOR FAMILY-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48125">
        <property type="entry name" value="LP07818P1"/>
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            <end position="33"/>
        </location>
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            <end position="1520"/>
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            <end position="146"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="518"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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</entry>
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</entry>
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</entry>
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    <accession>A0A6I8W7J9</accession>
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        <recommendedName>
            <fullName evidence="2">protein kinase C</fullName>
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        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Pkinase_C"/>
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    </dbReference>
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        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <text evidence="1">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
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            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
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        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
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    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="20">
        <location>
            <begin position="46"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="22">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021008"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031028"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_033240881.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="40464" checksum="219E5197193FE44E" modified="2020-08-12" version="1">KDKLIKLLGSYPIYKGYNPFFLIFNNSSKILFLTIIIIGTIITVTSNSWLGAXIGLEINLLSFIPLLRDNNNLTSTEASLKYFLTKALASTVLLFSSIILILKNNMNIEINESFTXIIIISALLLKIGAAPFHFXFPNIMEGLTXINALILITXQKIAPLILTSYLDIKNLLLIRVILSVIVGAIGGLNQTSLRKLIAFSSITHLGXILISLIIRESVXXIYFTFYSFLSFILTFIFNIFKLFHLNQLFSXFINRKVLKFTLFMNFLSLGGLPPFLRFXPKXLVIQELTFCNQYFTLTIIIISTLITLYXFILNYYENNXTINTQFNNINTNLYLICTFFSIFGLFLISLFFFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WED6</accession>
    <name>A0A6I8WED6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Piezo-type mechanosensitive ion channel component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Pzl</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the PIEZO (TC 1.A.75) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241796.1">
        <property type="nucleotide sequence ID" value="XM_033385905.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8WED6">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899548"/>
    <dbReference type="InParanoid" id="A0A6I8WED6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272429">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WED6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056769">
        <property type="entry name" value="Piezo_TM1-24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031805">
        <property type="entry name" value="Piezo_TM25-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056768">
        <property type="entry name" value="THU_Piezo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13167">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13167:SF25">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24871">
        <property type="entry name" value="Piezo_TM1-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15917">
        <property type="entry name" value="Piezo_TM25-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23188">
        <property type="entry name" value="THU_Piezo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="533"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="599"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="650"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="751"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="788"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="844"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="904"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="931"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="954"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1007"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1078"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1105"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1138"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1209"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1533"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1557"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1583"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1630"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1769"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1811"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1839"/>
            <end position="1862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1874"/>
            <end position="1891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1983"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo TM1-24" evidence="15">
        <location>
            <begin position="25"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo TM25-28" evidence="13">
        <location>
            <begin position="1063"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo transmembrane helical unit" evidence="14">
        <location>
            <begin position="1539"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo THU9 and anchor" evidence="16">
        <location>
            <begin position="1767"/>
            <end position="2005"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="12">
        <location>
            <begin position="2041"/>
            <end position="2328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1398"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1689"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2340"/>
            <end position="2367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1689"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="2340"/>
            <end position="2350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="2357"/>
            <end position="2367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033241796.1"/>
        </source>
    </evidence>
    <sequence length="2367" mass="278331" checksum="0534710646959A99" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>B5DHJ5</accession>
    <accession>A0A6I8UX05</accession>
    <name>B5DHJ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Forkhead box protein O-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6903491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132370.1">
        <property type="nucleotide sequence ID" value="XM_002132334.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DHJ5"/>
    <dbReference type="GeneID" id="6903491"/>
    <dbReference type="KEGG" id="dpo:6903491"/>
    <dbReference type="HOGENOM" id="CLU_2361949_0_0_1"/>
    <dbReference type="InParanoid" id="B5DHJ5"/>
    <dbReference type="OMA" id="TQDYFNG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246612">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002132370.1"/>
        </source>
    </evidence>
    <sequence length="96" mass="10579" checksum="8AC43EE4D17DE470" modified="2008-10-14" version="1">MKYFLSIIFGALSLTSAQYFGEDGIYGAAPNGPYGQQRPQQQQQAQTQDYFNGGFGYGYGPAFAAGLNQLQMQQQQQQLQQQQLDPYFYGGGPYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>B5DJR2</accession>
    <accession>A0A6I8UY26</accession>
    <name>B5DJR2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6902607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133133.1">
        <property type="nucleotide sequence ID" value="XM_002133097.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DJR2"/>
    <dbReference type="GeneID" id="6902607"/>
    <dbReference type="KEGG" id="dpo:6902607"/>
    <dbReference type="eggNOG" id="ENOG502T6RX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1295576_0_0_1"/>
    <dbReference type="InParanoid" id="B5DJR2"/>
    <dbReference type="OMA" id="CKSWMRR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250367">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002133133.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="27193" checksum="14073466A224DD91" modified="2008-10-14" version="1">MNPICRIDINLPECQKWMDDVYNKCREIFSREVASSAWWQDETPAIYKENIHAMTAIDCHKLKRDLAREVAATGTGSSAPTGPRSFLWWHKVILVVAYLLVFAALVFLYRCVNARCNGREHVREHGHGQGHVKIKEGTVAIEPDEALRPISRLEPKEKKEKSKKHWLRRKCRHVFVHSEAELRRQQAKREREERVHEEHVKHKIEKWTHARELEEQKRRDKKERSKSPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPV7</accession>
    <accession>A0A6I8V0W9</accession>
    <name>B5DPV7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Larval cuticle protein 65Ag1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135077.1">
        <property type="nucleotide sequence ID" value="XM_002135041.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPV7"/>
    <dbReference type="FunCoup" id="B5DPV7">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DPV7"/>
    <dbReference type="GeneID" id="6900714"/>
    <dbReference type="KEGG" id="dpo:6900714"/>
    <dbReference type="eggNOG" id="ENOG502ST8A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065450_7_3_1"/>
    <dbReference type="InParanoid" id="B5DPV7"/>
    <dbReference type="OMA" id="YESDVGP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245251">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF218">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 65AA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0193">Cuticle</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135077.1"/>
        </source>
    </evidence>
    <sequence length="104" mass="10907" checksum="E644C4CFA8A63D06" modified="2008-10-14" version="1">MKFLIVFVALFAVALAAPTGEVTILRSESDVGPENFSYAFETSDGTAAQESGVLKNAGSEQEAIAVQGSYKFIADDGVTYEVTYIADENGFQPSGAHLPVAPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-07-13" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D6QYE8</accession>
    <name>D6QYE8_9MUSC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8">
        <name type="scientific">Drosophila pseudoobscura</name>
        <dbReference type="NCBI Taxonomy" id="7237"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="Mol. Biol. Evol." volume="0" first="0" last="0">
            <title>Adaptive evolution of genes duplicated from the Drosophila pseudoobscura neo-X chromosome.</title>
            <authorList>
                <person name="Meisel R.P."/>
                <person name="Hilldorfer B.B."/>
                <person name="Koch J.L."/>
                <person name="Lockton S."/>
                <person name="Schaeffer S.W."/>
            </authorList>
            <dbReference type="PubMed" id="20351054"/>
            <dbReference type="DOI" id="10.1093/molbev/msq085"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">KB4 1100</strain>
            <strain evidence="9">KB5 1101</strain>
            <strain evidence="10">KB6 1102</strain>
            <strain evidence="11">KB8 1103</strain>
            <strain evidence="12">KB9 1104</strain>
            <strain evidence="13">MV19 1118</strain>
            <strain evidence="14">MV25 1121</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="HM017595">
        <property type="protein sequence ID" value="ADG64968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HM017596">
        <property type="protein sequence ID" value="ADG64969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HM017597">
        <property type="protein sequence ID" value="ADG64970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HM017598">
        <property type="protein sequence ID" value="ADG64971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HM017599">
        <property type="protein sequence ID" value="ADG64972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HM017602">
        <property type="protein sequence ID" value="ADG64975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HM017605">
        <property type="protein sequence ID" value="ADG64978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002132276.1">
        <property type="nucleotide sequence ID" value="XM_002132240.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D6QYE8"/>
    <dbReference type="SMR" id="D6QYE8"/>
    <dbReference type="GeneID" id="6903397"/>
    <dbReference type="KEGG" id="dpo:6903397"/>
    <dbReference type="OMA" id="HEVTTKC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246673">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03755">
        <property type="entry name" value="proteasome_alpha_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000059">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADG64968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADG64969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADG64970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADG64971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADG64972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADG64975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADG64978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <sequence length="249" mass="27996" checksum="ABCB0F23B966BD05" modified="2010-07-13" version="1">MSSRYDRAVTIFSPDGHLMQVEYAQEAVRKGSTAVGVRGGSCVVLAVEKKSVPKLQVDRTVRKICLLDQHIIMAFSGLTADARVLINRAQVECQTHRLNVEDQVTVEYITRYIAQIKQKYTQSNGRRPFGISCLIGGFDGDGTAHLFQTEPSGIFYEYKANATGRSAATVKEFFEKNYRDEDVITEQSAVKLAVRALLEVAQTSHNNLEVAIMEYNKPLKMLDRNAILDYVKIIEKDKEDALAMKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D38</accession>
    <accession>A0A6I8UC58</accession>
    <name>Q29D38_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine protease 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4812693</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353076.2">
        <property type="nucleotide sequence ID" value="XM_001353040.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D38"/>
    <dbReference type="SMR" id="Q29D38"/>
    <dbReference type="STRING" id="46245.Q29D38"/>
    <dbReference type="MEROPS" id="S01.B21"/>
    <dbReference type="GeneID" id="4812693"/>
    <dbReference type="KEGG" id="dpo:4812693"/>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="InParanoid" id="Q29D38"/>
    <dbReference type="OMA" id="TIISNTW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074857">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000164">
        <property type="entry name" value="Jonah 66Cii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="32"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353076.2"/>
        </source>
    </evidence>
    <sequence length="261" mass="28141" checksum="958B5BB085C7034F" modified="2008-10-14" version="2">MRLSCAILLLASALAFASGSPTFNRTERSDRIVNGYPAYEGKAPYIVGLFLLTDGSNSAAVGAGTIISNTWVLTAAHCLTTDSVDINYGSNWAWNGLYKHNVRRDNFILHPGFGNSAGHDIGLIRTPYVEFTNVVSNIPLPKFNQQGDRFEGWWCVACGWGGMDNGNLAEWLQCIDVQIISNNECAQSYGSVASSDMCTRQSDGRSVCGGDSGGPLVTQDNPMLVGVITFASGNCKSGPSGYTRVTDHLEWIRDNSGVAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DW4</accession>
    <accession>A0A6I8UBD9</accession>
    <name>Q29DW4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Retinoid-inducible serine carboxypeptidase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Serine carboxypeptidase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4811919</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in vascular wall and kidney homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352800.2">
        <property type="nucleotide sequence ID" value="XM_001352764.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DW4"/>
    <dbReference type="SMR" id="Q29DW4"/>
    <dbReference type="FunCoup" id="Q29DW4">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DW4"/>
    <dbReference type="ESTHER" id="drops-q29dw4">
        <property type="family name" value="Carboxypeptidase_S10"/>
    </dbReference>
    <dbReference type="MEROPS" id="S10.A52"/>
    <dbReference type="GeneID" id="4811919"/>
    <dbReference type="KEGG" id="dpo:4811919"/>
    <dbReference type="eggNOG" id="KOG1283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008523_1_0_1"/>
    <dbReference type="InParanoid" id="Q29DW4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076951">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DW4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000075">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001352800.2"/>
        </source>
    </evidence>
    <sequence length="441" mass="49454" checksum="6D1BD44781CFBF36" modified="2016-01-20" version="2">MKMTSLVVIFLACFAALAHGKAGYGPGEQDWGFVDVRPGAHMFYWLYYTTANVTSYTERPLAIWLQGGPGASSTGYGNFEELGPVDLYGDYRNWTWVKDMNVMFIDNPVGSGYSYVDGTAYHTATNREIALDLVELMKGFYKLHPEFEAVPLHIFCESYGGKMAPEFALELYYAKERGEVKSNLTSVALGDPWTSPIDSVLAWGPMLREMGIVDHDGYDAITAAANFTAELVAEERWIQATAQWGNTQWEVMKASKGVDFYNVLKETLGDRFQRQLAMTPEERQYRTMVKFDIDEDRDQLLEDLMRGPVAETLGIPSNVKWGSQSSSTFDIHRTDFMKPVIHIVNELLEKTPLKVGVFSGGLDLICATPGTVNWIAKLDWSRKSEYLAASRTAISVDRILEGYQKTGGNFTMFWINRSGHMAPADNPAAMSHVLREFTTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E32</accession>
    <accession>A0A6I8UB73</accession>
    <name>Q29E32_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cysteine and histidine-rich protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4812539</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZFTRAF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352731.2">
        <property type="nucleotide sequence ID" value="XM_001352695.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E32"/>
    <dbReference type="FunCoup" id="Q29E32">
        <property type="interactions" value="690"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E32"/>
    <dbReference type="GeneID" id="4812539"/>
    <dbReference type="KEGG" id="dpo:4812539"/>
    <dbReference type="eggNOG" id="KOG3002">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035849_0_1_1"/>
    <dbReference type="InParanoid" id="Q29E32"/>
    <dbReference type="OMA" id="HERPEHE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076954">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16505">
        <property type="entry name" value="RING-HC_CYHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039338">
        <property type="entry name" value="ZFTRAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23059">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23059:SF4">
        <property type="entry name" value="ZINC FINGER TRAF-TYPE-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001352731.2"/>
        </source>
    </evidence>
    <sequence length="411" mass="45382" checksum="036220174B1C1C9C" modified="2008-10-14" version="2">MSNVESSSSAAQQPPTSTLPMLGDNQVMQASTSASSTSNSSTSSSSGNGGGGGGGNNVVGVPLDTQISGEPPSKKPCLDAASSSSGGALSSAAAASGTHEKLAYRISTALCCAVCLDLPKTAMYQCQMGHLMCAACFTHLLADGRLRDQIATCPNCRVEISKSTASRNLAVEKAASELPSECQFCNKEFPYKSLERHEQHECQERPTKCKYHRIGCQWRGPYHETTEHERNCSHPKKSGYEVMAALEAHDLKIKEEKKMFNTLIDLLSYEKIIFNDLQMKPYRTDEYVHKLFYETARFSAFNQQWVVKARINNSQRDPHQSNERTITYQLILKTKTSTPMSIHFFALKGPFSDMKVSTQIYKHEFTETNTESDYYVLPLPDGIGGSGSSECNRMLANKGINFRLLMFLLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E80</accession>
    <accession>A0A6I8UB13</accession>
    <name>Q29E80_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein RhoGEF4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RhoGEF4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352683.1">
        <property type="nucleotide sequence ID" value="XM_001352647.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E80"/>
    <dbReference type="SMR" id="Q29E80"/>
    <dbReference type="FunCoup" id="Q29E80">
        <property type="interactions" value="782"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E80"/>
    <dbReference type="GeneID" id="4812588"/>
    <dbReference type="KEGG" id="dpo:4812588"/>
    <dbReference type="eggNOG" id="KOG4424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_425963_0_0_1"/>
    <dbReference type="InParanoid" id="Q29E80"/>
    <dbReference type="OMA" id="RKCEYET"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081190">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29E80">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000548">
        <property type="entry name" value="GD13075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000048">
        <property type="entry name" value="GL21162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF159">
        <property type="entry name" value="LD03170P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352683.1"/>
        </source>
    </evidence>
    <sequence length="651" mass="74175" checksum="C9D094AC53E8CDDC" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FH1</accession>
    <accession>A0A6I8UEQ7</accession>
    <name>Q29FH1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4814081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354231.2">
        <property type="nucleotide sequence ID" value="XM_001354195.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FH1"/>
    <dbReference type="GeneID" id="4814081"/>
    <dbReference type="KEGG" id="dpo:4814081"/>
    <dbReference type="HOGENOM" id="CLU_1837190_0_0_1"/>
    <dbReference type="InParanoid" id="Q29FH1"/>
    <dbReference type="OMA" id="RMASIQF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072651">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354231.2"/>
        </source>
    </evidence>
    <sequence length="140" mass="15564" checksum="2A3D281EA9A5FBE0" modified="2008-10-14" version="2">MALKPQRSATSVSFAQPKQGRRASAGPDEINNTYIKQIVNDWRNSVRERRFTTDLDDSATSSATELPRANSKKSITTTSIIRPSIQRPHLLRNYSKPVDIEHGKGSAEADRIVVAEGIDLYIHQPIKVGFSKPLQAPKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HS0</accession>
    <accession>A0A6I8UFG7</accession>
    <name>Q29HS0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Corp</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Corp</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354634.2">
        <property type="nucleotide sequence ID" value="XM_001354598.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HS0"/>
    <dbReference type="GeneID" id="4815257"/>
    <dbReference type="KEGG" id="dpo:4815257"/>
    <dbReference type="eggNOG" id="ENOG502TD6F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1300844_0_0_1"/>
    <dbReference type="InParanoid" id="Q29HS0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070729">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HS0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354634.2"/>
        </source>
    </evidence>
    <sequence length="215" mass="25025" checksum="E30CF01F259D89F7" modified="2016-01-20" version="2">MLNMTDIKSCLKICFQGETSFIICEAENSYEEVIVQAMARFGIPETQKEALLLTNGNGYIFEPQLLEYFILLFPCPEITFHLRLSWQKLRRGHSLNQSIKRKRHTDQETTPTEQTLEQEQGKKEHQEEEYKPVAVYRQNCFLGSLPSSEAAPVRRQNCFLRERQPQQQLQREAEIHASREEPQQPQAKRLRLDVCAKPRRAASIAPLPAMRSIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JJ9</accession>
    <accession>A0A6I8UGS2</accession>
    <name>Q29JJ9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 26</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">VPS26 protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Vps26</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS26 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355245.2">
        <property type="nucleotide sequence ID" value="XM_001355209.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JJ9"/>
    <dbReference type="FunCoup" id="Q29JJ9">
        <property type="interactions" value="2189"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JJ9"/>
    <dbReference type="GeneID" id="4815559"/>
    <dbReference type="KEGG" id="dpo:4815559"/>
    <dbReference type="eggNOG" id="KOG3063">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029884_1_0_1"/>
    <dbReference type="InParanoid" id="Q29JJ9"/>
    <dbReference type="OMA" id="HQHVPLH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073297">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="302"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="450"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001355245.2"/>
        </source>
    </evidence>
    <sequence length="522" mass="56986" checksum="0E99A5A786816F25" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JM5</accession>
    <accession>A0A6I8UGQ5</accession>
    <name>Q29JM5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4816095</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355219.2">
        <property type="nucleotide sequence ID" value="XM_001355183.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JM5"/>
    <dbReference type="FunCoup" id="Q29JM5">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JM5"/>
    <dbReference type="GeneID" id="4816095"/>
    <dbReference type="KEGG" id="dpo:4816095"/>
    <dbReference type="eggNOG" id="ENOG502RZA8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064151_1_0_1"/>
    <dbReference type="InParanoid" id="Q29JM5"/>
    <dbReference type="OMA" id="NPFFQVE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078262">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JM5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038757">
        <property type="entry name" value="BRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35259">
        <property type="entry name" value="BOMBESIN RECEPTOR-ACTIVATED PROTEIN C6ORF89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35259:SF1">
        <property type="entry name" value="BOMBESIN RECEPTOR-ACTIVATED PROTEIN C6ORF89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001355219.2"/>
        </source>
    </evidence>
    <sequence length="455" mass="52539" checksum="2F0B858BCCA1E15F" modified="2008-10-14" version="2">MQAEIVTASDRVNDSPPQTRKKATKTTTDVETDETRRKRKAMDDYDIDIDLNFVQLSRQQLLQRYSTAVRKLRRLAEAHHEVRPLSFDSYVLFQYFQQRNWRQRMAGMGGGSATLVPPPQLMAYLKLEVLHHLLDRRRQILCWLFYLTLVSLCVAAYRYETSSGSNERVVQSTVYPGMRMWRRMTMPLIQRFPRLTEFYDESCLMGNPFFQMEDLSCGPCANVESVWFESDECAQLHAPVSGSDKGLSLLLKCRQTMAEGDSVPFAFKSQQDAMVLSEFYNIYTSNHMIFQRDAYRVHSTNQGVHNLEDLFGQFNSSNSDMNTNVDPPTWTQQAHNLWRCNRMLPARLLRPIFARPSRLPSLGVALERYVAIDTAQAPAYTLPETECPNVYVHQAVGTRFIILRPTSECRHRCRTLSIRLTQSFVLSYNWLYWKPISAPDPISESLSISLIGSYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KV7</accession>
    <accession>A0A6I8UII0</accession>
    <name>Q29KV7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloendopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001356009.2">
        <property type="nucleotide sequence ID" value="XM_001355973.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KV7"/>
    <dbReference type="SMR" id="Q29KV7"/>
    <dbReference type="STRING" id="46245.Q29KV7"/>
    <dbReference type="MEROPS" id="M12.A10"/>
    <dbReference type="GeneID" id="4816542"/>
    <dbReference type="KEGG" id="dpo:4816542"/>
    <dbReference type="eggNOG" id="KOG3714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017286_2_3_1"/>
    <dbReference type="InParanoid" id="Q29KV7"/>
    <dbReference type="OMA" id="GAQRNIW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073639">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KV7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000037">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF814">
        <property type="entry name" value="MEPRIN A SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2 4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035543879" description="Metalloendopeptidase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356009.2"/>
        </source>
    </evidence>
    <sequence length="250" mass="28957" checksum="F84413076AE70089" modified="2008-10-14" version="2">MHGPLILSLVLLLSRSIRGAPVERLETDPELTGGYIEGDMVPDGAQRNIWRNETYRWPNRIVYYHINSYIDQEHRNQIVRAIQKIESVSCLTFREASTDQKYYVNVTSEEGGCYSYIGHLDRVQQLNLQPYEVGTGCFRLYTIVHEFLHALGFFHQQSAANRDEYVRIVEENITEGMEFNFDKYSAEMVNDFDAEYDYGSVMHYGPLAFSKNNESTIVPLQEGMQEVIGQRLQLSQTDIDKLNAMYRCPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29M76</accession>
    <accession>A0A6I8UK30</accession>
    <name>Q29M76_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Heat shock factor-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4816832</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Negative regulator of the heat shock response. Negatively affects HSF1 DNA-binding activity. May have a role in the suppression of the activation of the stress response during the aging process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer. Associates with heptad repeats of HSF1 trimers and probably also HSF1 monomers, and with HSP70. Association with HSF1 trimers and HSP70 coincides with attenuation of heat shock response and the conversion of HSF1 trimer to monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSBP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356753.1">
        <property type="nucleotide sequence ID" value="XM_001356717.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29M76"/>
    <dbReference type="FunCoup" id="Q29M76">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29M76"/>
    <dbReference type="GeneID" id="4816832"/>
    <dbReference type="KEGG" id="dpo:4816832"/>
    <dbReference type="eggNOG" id="KOG4117">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149552_3_0_1"/>
    <dbReference type="InParanoid" id="Q29M76"/>
    <dbReference type="OMA" id="MERANMD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078887">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29M76">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.430:FF:000002">
        <property type="entry name" value="Heat shock factor-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009643">
        <property type="entry name" value="HS1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424:SF0">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06825">
        <property type="entry name" value="HSBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356753.1"/>
        </source>
    </evidence>
    <sequence length="88" mass="10110" checksum="2F5225534DE0E252" modified="2006-04-04" version="1">MTELRNEMDSDLDQNYSLNSNAAMDPKNMQELTIYVQNLLQNVQDKFQTMSDQIITRIDDMGNRIDDLEKSIADLMNQAGIEGQIPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PP1</accession>
    <accession>A0A6I8UKZ9</accession>
    <name>Q29PP1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Prefoldin subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Pfdn1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357183.1">
        <property type="nucleotide sequence ID" value="XM_001357147.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PP1"/>
    <dbReference type="FunCoup" id="Q29PP1">
        <property type="interactions" value="2139"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29PP1"/>
    <dbReference type="GeneID" id="4818056"/>
    <dbReference type="KEGG" id="dpo:4818056"/>
    <dbReference type="eggNOG" id="KOG3501">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122140_2_1_1"/>
    <dbReference type="InParanoid" id="Q29PP1"/>
    <dbReference type="OMA" id="REMIQQK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072728">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29PP1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357183.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="14831" checksum="37A9A5A5CF95CDF0" modified="2006-04-04" version="1">MAQMDIELKKAFTEMQINKLETSKKINMIDMKCDMVKTGKHKYLLTEKGTNNLDNDTRVYMSVGRMFLLTDVQNMREDLKIKQEKCEKAIELLEKKKEFLQKSLKDQEDGLRELVQQRKEADVVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P1N4</accession>
    <accession>A0A6I8V9V4</accession>
    <name>A0A0R3P1N4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">DNA topoisomerase 2</fullName>
            <ecNumber evidence="5 15">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Top2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14 15">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5 15"/>
    <dbReference type="RefSeq" id="XP_015036828.1">
        <property type="nucleotide sequence ID" value="XM_015181342.2"/>
    </dbReference>
    <dbReference type="SMR" id="A0A0R3P1N4"/>
    <dbReference type="FunCoup" id="A0A0R3P1N4">
        <property type="interactions" value="1636"/>
    </dbReference>
    <dbReference type="GeneID" id="4816595"/>
    <dbReference type="KEGG" id="dpo:4816595"/>
    <dbReference type="InParanoid" id="A0A0R3P1N4"/>
    <dbReference type="OMA" id="TWTQDFK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070228">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.268.10:FF:000013">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000003">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13 14 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14 15" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="17">
        <location>
            <begin position="434"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="18">
        <location>
            <begin position="694"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1071"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1182"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1246"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1080"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1253"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1297"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1342"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1419"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1433"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="14">
        <location>
            <position position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015036828.1"/>
        </source>
    </evidence>
    <sequence length="1450" mass="164865" checksum="B9EDF9283EFBB352" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P6Y3</accession>
    <accession>A0A6I8VJ50</accession>
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        <submittedName>
            <fullName evidence="7">Oocyte zinc finger protein XlCOF8.4-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6900961</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042428.1">
        <property type="nucleotide sequence ID" value="XM_015186942.2"/>
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    <dbReference type="GeneID" id="6900961"/>
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        <property type="component" value="Chromosome X"/>
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    <dbReference type="Bgee" id="FBgn0245924">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
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    </dbReference>
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        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
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    <dbReference type="PANTHER" id="PTHR19818:SF139">
        <property type="entry name" value="PAIR-RULE PROTEIN ODD-PAIRED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
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    </dbReference>
    <dbReference type="Pfam" id="PF00096">
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    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
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            <begin position="168"/>
            <end position="183"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015042428.1"/>
        </source>
    </evidence>
    <sequence length="527" mass="60240" checksum="A5EA5CE627BB5C62" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAU4</accession>
    <name>A0A6I8UAU4_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Adult-specific rigid cuticular protein 15.7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Cpr72Eb</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352569.2">
        <property type="nucleotide sequence ID" value="XM_001352533.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAU4"/>
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        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812592"/>
    <dbReference type="InParanoid" id="A0A6I8UAU4"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF196">
        <property type="entry name" value="CUTICULAR PROTEIN 72EA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0193">Cuticle</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026310587" evidence="2">
        <location>
            <begin position="23"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352569.2"/>
        </source>
    </evidence>
    <sequence length="229" mass="25137" checksum="4A26A27FDE0EAB22" modified="2020-08-12" version="1">MSKMHSFNLCLMIATATSLVCGSPLLEYGAPPAGDSVSQYHTQDEHGQYAYGYMAPLYSKHETRTADGVTRGTFSYVDARGVTQTVDYVADTEGFRVSSSLSQQQANEETPEVAALRAQHLAAHAQAKLRLAGGDSYAGSVELPQPVQDTPEVAAAKVAFFKRFEAEKLRNQLLSEKQINRVIAPSIPVRSQPLYVYQPLTGFVYNYHQSKSKAQAKTQTPSRDYLPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAZ4</accession>
    <name>A0A6I8UAZ4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibrous sheath CABYR-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352587.2">
        <property type="nucleotide sequence ID" value="XM_001352551.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAZ4"/>
    <dbReference type="FunCoup" id="A0A6I8UAZ4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812138"/>
    <dbReference type="InParanoid" id="A0A6I8UAZ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004011">
        <property type="entry name" value="Gyr_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004019">
        <property type="entry name" value="YLP_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02756">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02757">
        <property type="entry name" value="YLP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00713">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026353157" description="Fibrous sheath CABYR-binding protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352587.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27077" checksum="FC06070C43560525" modified="2020-08-12" version="1">MKIFSLGSVLALSAVASAALAAPQLESRLRRDVSELVDPAGAAPVEYLPPGITEAALLEDVVEAEADPQGTALGGEGYEYRTVRRLKLRHRNRRDVSHLQVAPARQYLPPSQAYLPPAPAAPAAPVAAPIDDTEEVVSAAEPKLALAREYLPPVEVSTEAAPQEETTPLPEEPEADVRVVDVPTNGAGQLLDDGYHYQQPVEMPAELREAAREYLPPLEDEAVVVEGPADGQSAVLTNDGYQYRAVRRYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB81</accession>
    <name>A0A6I8UB81_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Probable chitinase 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Cht7</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352733.4">
        <property type="nucleotide sequence ID" value="XM_001352697.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UB81"/>
    <dbReference type="FunCoup" id="A0A6I8UB81">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812499"/>
    <dbReference type="InParanoid" id="A0A6I8UB81"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02872">
        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.140.10:FF:000007">
        <property type="entry name" value="Acidic mammalian chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000007">
        <property type="entry name" value="Acidic mammalian chitinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026358027" evidence="9">
        <location>
            <begin position="27"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="124"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="559"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="10">
        <location>
            <begin position="949"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001352733.4"/>
        </source>
    </evidence>
    <sequence length="1014" mass="113003" checksum="85EDD1EF5DD5338D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB91</accession>
    <name>A0A6I8UB91_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352712.3">
        <property type="nucleotide sequence ID" value="XM_001352676.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UB91"/>
    <dbReference type="KEGG" id="dpo:4812002"/>
    <dbReference type="InParanoid" id="A0A6I8UB91"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48174">
        <property type="entry name" value="DUF946 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48174:SF5">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026106967" description="Vacuolar protein sorting-associated protein 62" evidence="2">
        <location>
            <begin position="25"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352712.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="62917" checksum="E734CE1A6745295D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBJ2</accession>
    <name>A0A6I8UBJ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Dyslexia-associated protein KIAA0319</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4812454</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352876.3">
        <property type="nucleotide sequence ID" value="XM_001352840.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBJ2"/>
    <dbReference type="FunCoup" id="A0A6I8UBJ2">
        <property type="interactions" value="805"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812454"/>
    <dbReference type="InParanoid" id="A0A6I8UBJ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBJ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001655">
        <property type="entry name" value="Blast:Dyslexia-associated protein KIAA0319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000061">
        <property type="entry name" value="Dyslexia-associated protein KIAA0319 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000257">
        <property type="entry name" value="Dyslexia-associated protein KIAA0319-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002264">
        <property type="entry name" value="dyslexia-associated protein KIAA0319-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029865">
        <property type="entry name" value="KIAA0319-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013980">
        <property type="entry name" value="MANSC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011106">
        <property type="entry name" value="MANSC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46182">
        <property type="entry name" value="FI19480P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46182:SF2">
        <property type="entry name" value="FI19480P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22352">
        <property type="entry name" value="K319L-like_PKD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00765">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50986">
        <property type="entry name" value="MANSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026117706" evidence="11">
        <location>
            <begin position="21"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="977"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="MANSC" evidence="12">
        <location>
            <begin position="70"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="322"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1050"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="322"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1071"/>
            <end position="1082"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001352876.3"/>
        </source>
    </evidence>
    <sequence length="1100" mass="119669" checksum="942EA17AAFC619AA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBL2</accession>
    <name>A0A6I8UBL2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine protease nudel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ndl</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352853.3">
        <property type="nucleotide sequence ID" value="XM_001352817.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UBL2">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812515"/>
    <dbReference type="InParanoid" id="A0A6I8UBL2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBL2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000111">
        <property type="entry name" value="Blast:Serine protease nudel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000201">
        <property type="entry name" value="Serine protease nudel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000210">
        <property type="entry name" value="Serine protease nudel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015420">
        <property type="entry name" value="Peptidase_S1A_nudel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF116">
        <property type="entry name" value="FI16631P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09342">
        <property type="entry name" value="DUF1986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="1072"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="1902"/>
            <end position="2176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="339"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="413"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="467"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="753"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1402"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1525"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="420"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="766"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1410"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1525"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="842"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="919"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="1323"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="1342"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="1633"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="1671"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="2190"/>
            <end position="2208"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="2317"/>
            <end position="2332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352853.3"/>
        </source>
    </evidence>
    <sequence length="2491" mass="277222" checksum="CAD447725A1113C4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UD13</accession>
    <name>A0A6I8UD13_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4794 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4812812</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353509.2">
        <property type="nucleotide sequence ID" value="XM_001353473.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UD13"/>
    <dbReference type="FunCoup" id="A0A6I8UD13">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812812"/>
    <dbReference type="InParanoid" id="A0A6I8UD13"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032011">
        <property type="entry name" value="DUF4794"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16042">
        <property type="entry name" value="DUF4794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026190430" description="DUF4794 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4794" evidence="3">
        <location>
            <begin position="36"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353509.2"/>
        </source>
    </evidence>
    <sequence length="286" mass="30648" checksum="C4FF3F91518A9757" modified="2020-08-12" version="1">MKSISILILAAALAVATVSAMPQRRLKSQRQVEYASTPYPAAGFRPRIPFALPSEQQPKLEAEPLTTPSAVETLENAETTTTEVAQQQEAETETEAELEVALRTPANTYGAPDDQQDQDTVEVVAQAPARDFQPPVLEAVEDFAADGAATADGQQPVADVPALGQGEEEARQLDEPEPEPIPTDVEVKAVTPAGTYGPPPSPKPASTYGAPELSEPENELDLEESQVELVGEQEELEGLDGSLANELASGRLILLPLGSQGAQFGRLILAVEQPRQRSRSERLRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDK1</accession>
    <name>A0A6I8UDK1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Basic helix-loop-helix neural transcription factor TAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tap</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353803.2">
        <property type="nucleotide sequence ID" value="XM_001353767.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDK1"/>
    <dbReference type="FunCoup" id="A0A6I8UDK1">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813439"/>
    <dbReference type="InParanoid" id="A0A6I8UDK1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11428">
        <property type="entry name" value="bHLH_TS_NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000006">
        <property type="entry name" value="Neurogenic differentiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF163">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX NEURAL TRANSCRIPTION FACTOR TAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="158"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="105"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="263"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="365"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353803.2"/>
        </source>
    </evidence>
    <sequence length="412" mass="46517" checksum="5F467E1A24EB154B" modified="2020-08-12" version="1">MAACYNAYSAPSQSFEFDEDDDDASFDSGYEKSFETEAQVSSRRRLDFDETYNSAAAGPAAPLAYAGTWDTAPINSPPAGFVGLLDTSSNHSTRSGRTLVEHLNSRATGGGFEPQLTSTPMKSPEDPDAPRPKRKYAVGKNRVTRSRSPTQVVRIKKFRRMKANDRERNRMHSLNDALEKLRVTLPSLPEETKLTKIEILRFAHNYIFALEQVLESGNTVNLDLEKLQNFTLSGERITKELFDALFVNPQPFPMFGCGRMFPYGQTQQPPLSQHSEAHSDPLPHPHHHQPQMVPFQPGMDYPSAAPHPHPQHPTGFDFGSSMRYYQHHPQQEPNPQQTPPAGQFSQEKYDLFRGSFEAAANLPSSATEPAMHQQSSFYTQTPPWKEYPEEQPNHHPQLHPYPHSYKQFAPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDU2</accession>
    <name>A0A6I8UDU2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Endocuticle structural protein SgAbd-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Cpr65Aw</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353832.3">
        <property type="nucleotide sequence ID" value="XM_001353796.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDU2"/>
    <dbReference type="FunCoup" id="A0A6I8UDU2">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813245"/>
    <dbReference type="InParanoid" id="A0A6I8UDU2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF218">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 65AA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026214814" evidence="3">
        <location>
            <begin position="27"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353832.3"/>
        </source>
    </evidence>
    <sequence length="120" mass="12954" checksum="F7AF0A41BA9EDEF4" modified="2020-08-12" version="1">MARQTLIPILAGFCLCLCLCFSDADAASDGSNAAQILKYENEKMDGDGYAFSFETSDGISRQETATLKHPGTPLEAIAVQGSVNWVGPDGEHYKLNYLADENGFQPQGEHLPQLEATRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDW2</accession>
    <name>A0A6I8UDW2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 19">Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase</fullName>
            <ecNumber evidence="5 19">2.4.1.135</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">GlcAT-P</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21 22">MV-25-SWS-2005</strain>
            <tissue evidence="21 22">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 19">
            <text>3-O-(beta-D-galactosyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24168"/>
            <dbReference type="Rhea" id="RHEA-COMP:12571"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132090"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="EC" id="2.4.1.135"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17 19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Golgi apparatus membrane</location>
            <topology evidence="2 19">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.135" evidence="5 19"/>
    <dbReference type="RefSeq" id="XP_001353933.3">
        <property type="nucleotide sequence ID" value="XM_001353897.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043332.2">
        <property type="nucleotide sequence ID" value="XM_015187846.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDW2"/>
    <dbReference type="KEGG" id="dpo:4813756"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDW2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00218">
        <property type="entry name" value="GlcAT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000044">
        <property type="entry name" value="Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF50">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 17 19" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="16">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="18">
        <location>
            <position position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001353933.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_015043332.2"/>
        </source>
    </evidence>
    <sequence length="470" mass="50840" checksum="829838253BB60421" modified="2020-08-12" version="1">MKGGGAYISIGSSTSGINSMGSGSGSGSGSKMTLGKSVKMYLTIFIATTCIYMVLYQYHMSREPFAPSEVGKHQEKSSSSSYIASYLWSPMSLLMANATTTTTTTTVSSTTTTTATVSNAGQVGQGSSSGSIRTVSLAATRPTQASSLASASASAGGAAAHKTAAATTTTTSTTPPATVTTTTTTRANPIAKKGPASTSMSQSQLDAAHKIRPTFVAASEPPPLYIITPTYRRPEQLAELTRLGYTLKHVANLLWLVIEDANKTNPLVAHTLDRIGVPYEYMVAPMPEKYKQTKKAKPRGVSNRNRGLEYLRQHATEGVLYFADDDNTYDISIFEQMRYISKVGMWPVGLVTKTGVSSPIIRNGKLDGYYDGWIGGRKYPVDMAGFAVNIKFLRQRPHAQMPFKPGYEEDGFLRSLAPLDNDEIDLLADECRDILTWHTQTKKNPPAQALNKTRYSNTNLVHIDKLLVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE51</accession>
    <name>A0A6I8UE51_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Proton-coupled amino acid transporter-like protein CG1139</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">acs</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354024.4">
        <property type="nucleotide sequence ID" value="XM_001353988.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE51"/>
    <dbReference type="FunCoup" id="A0A6I8UE51">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812787"/>
    <dbReference type="InParanoid" id="A0A6I8UE51"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF340">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="47"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354024.4"/>
        </source>
    </evidence>
    <sequence length="456" mass="50601" checksum="BC5FF4D72BA63C8E" modified="2020-08-12" version="1">MQTDTIKTVAIIYPTTTNGESTGPTKPSNVSSDDYDPHLNRNVKNPTTNWQTFAHFLKASVGTGVLAMPSAFSHAGYVNGTILTLIIGLLALYCLHILINCMYILCKRQKVPYVSFSEAMNLGLKQGPPWLRCLAPIAIPFVDGFLAFYHFGICCVYVVFIAESIKQLVDEYLVVWDVRLHMCLLIVPLLLIYSIKNLQVLAPFSSAANLLLLVGFGIILYYIFEDLPPLSERDAFVSYTELPTFFGTVLFALEAVGVILAIEENMATPGDFVKPCGIMNWGMSIVLGLYVLLGFFGYWKYGDDALGSITLNIPQWDIPAQVVKIFFAITTWISYALQGYVTAHILWGKYVSTRIKNTKRHSLYELIFRAIIVLLTFACAVAIPDLSVFLSLVGSFCLSILGLIFPALLQICVQYEHGYGPLRIKLIINILLLCFGVFGGVVGTYVSILEIIAVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE84</accession>
    <name>A0A6I8UE84_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="5">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">AcCoAS</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001354073.2">
        <property type="nucleotide sequence ID" value="XM_001354037.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE84"/>
    <dbReference type="FunCoup" id="A0A6I8UE84">
        <property type="interactions" value="1150"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813796"/>
    <dbReference type="InParanoid" id="A0A6I8UE84"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UE84">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000004">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF244">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="97"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="552"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001354073.2"/>
        </source>
    </evidence>
    <sequence length="670" mass="76142" checksum="BBCC66240843181E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEC8</accession>
    <name>A0A6I8UEC8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Probable serine hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4813218</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354080.2">
        <property type="nucleotide sequence ID" value="XM_001354044.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEC8"/>
    <dbReference type="KEGG" id="dpo:4813218"/>
    <dbReference type="InParanoid" id="A0A6I8UEC8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF14">
        <property type="entry name" value="SERINE HYDROLASE-LIKE PROTEIN DDB_G0286239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="58"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354080.2"/>
        </source>
    </evidence>
    <sequence length="330" mass="38197" checksum="837FF621F9125C24" modified="2020-08-12" version="1">MEVLRHLRLLTKLTNVRHASSGATRPLKVLTKHFDEVSIPVPWGHIAGKWYGPKHVRPIVGMHGWQDNAGTFDTLAPLLPNHISFLSIDMPGHGLSSWLPPGISYHSIDYVLVTRRLMEEYNWDKISMMAHSMSSINGFVFCALFPEKVDMFIGLDVLKPPVRSARHIVDGLSERLEATLKLERRLRSGSEPPCYEWDELEKRLHEGSNKSVTLESCKYLLQRNCKASTHEPHKYYFSRDNRLKTSLFYTLHNEVPLEMASRIKCPHLFIKALQAPYYERKEYYDATLAELRKNPLFEYNELDGTHHVHLNEPEKVAPIINSFINKYRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UID4</accession>
    <name>A0A6I8UID4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloendopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4816506</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001356011.2">
        <property type="nucleotide sequence ID" value="XM_001355975.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UID4"/>
    <dbReference type="KEGG" id="dpo:4816506"/>
    <dbReference type="InParanoid" id="A0A6I8UID4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000037">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF814">
        <property type="entry name" value="MEPRIN A SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2 5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026379948" description="Metalloendopeptidase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="60"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356011.2"/>
        </source>
    </evidence>
    <sequence length="262" mass="29058" checksum="285A22C7B4E258A9" modified="2020-08-12" version="1">MSRLGLIVVLLVSVRLGQGKPLPLPAGVYDPEEAGGFAGGDMVLTEEQQADLLHGPKARNGLVNTARRWPNNVVVYKISEDFDATHREAILKGIETLEENSCVKFREATDQDTAYLTITANVGGCYTDVGYRAEPQEMNLEIYPIGQGCFRPGTILHEFMHALGFYHEQSSALRDDYVDVVYDNIVPGKEFNFQKYSESLVTDFDVGYDYDSCLHYRPGAFSINGEDTIVPLDPGAVIGQRLGLSPKDIDKINIMYKCPILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIG4</accession>
    <name>A0A6I8UIG4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine-rich repeat protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Lrr47</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356045.3">
        <property type="nucleotide sequence ID" value="XM_001356009.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIG4"/>
    <dbReference type="SMR" id="A0A6I8UIG4"/>
    <dbReference type="FunCoup" id="A0A6I8UIG4">
        <property type="interactions" value="948"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816486"/>
    <dbReference type="InParanoid" id="A0A6I8UIG4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057437">
        <property type="entry name" value="PIF1/LRR1_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25344">
        <property type="entry name" value="PH_LRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PIF1/LRR1 pleckstrin homology" evidence="4">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001356045.3"/>
        </source>
    </evidence>
    <sequence length="428" mass="47695" checksum="9AB8F489C6B43D01" modified="2020-08-12" version="1">MKILCEVQVVNRTTQANRTPRPVKSTLAIGYKQGASDSNGNTKKELEMLLFSGTTKTGTRYKVRDNVQAVHTKFILDGKATIGFHQPAENLLIKCDPIQLKGFLQTLKLGMEGKDAINLRLNIAAATAIPQKSQPQQRMVVSKRSEYPIKGFPRTLKSLTINNSQLVKLSFEICTLRNLTTLDVGGNKLTKIPPELGRLPLTTLQLSGNCLGELNDWCWMRGTKLSQSLRELDLSSNGLTYFPPALVKFERLVSLKLNHNQLTRLPFAIRRLQGLRSLHVCSNKLESLPAAIEDLRIDLLDVWGNCFRGFNAEAAQLQSQRLSASNTPQPLWLQAARAVANYKLPLSADSVPAILIELISEAPKCLCGKLCYALRQEDLLQRVIQPKFTNIKNLTYSREHQIYADVVICGPNCSASQQLKALFALMFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIL4</accession>
    <name>A0A6I8UIL4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein nessun dorma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nesd</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356105.3">
        <property type="nucleotide sequence ID" value="XM_001356069.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIL4"/>
    <dbReference type="FunCoup" id="A0A6I8UIL4">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816703"/>
    <dbReference type="InParanoid" id="A0A6I8UIL4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007112">
        <property type="term" value="P:male meiosis cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057508">
        <property type="entry name" value="SHCBP-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045140">
        <property type="entry name" value="SHCBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14695:SF4">
        <property type="entry name" value="PROTEIN NESSUN DORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14695">
        <property type="entry name" value="SHC SH2-DOMAIN BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23762">
        <property type="entry name" value="SHCBP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHC SH2" evidence="1">
        <location>
            <begin position="29"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356105.3"/>
        </source>
    </evidence>
    <sequence length="603" mass="68266" checksum="8A2E31B3A7732F8E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIM0</accession>
    <name>A0A6I8UIM0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Low-density lipoprotein receptor-related protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4816498</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356063.3">
        <property type="nucleotide sequence ID" value="XM_001356027.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIM0"/>
    <dbReference type="FunCoup" id="A0A6I8UIM0">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816498"/>
    <dbReference type="InParanoid" id="A0A6I8UIM0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIM0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013980">
        <property type="entry name" value="MANSC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011106">
        <property type="entry name" value="MANSC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46876">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46876:SF1">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07502">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00765">
        <property type="entry name" value="MANEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50986">
        <property type="entry name" value="MANSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026091953" evidence="11">
        <location>
            <begin position="24"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="602"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="MANSC" evidence="12">
        <location>
            <begin position="54"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="292"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="441"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="504"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="444"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="533"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001356063.3"/>
        </source>
    </evidence>
    <sequence length="653" mass="71064" checksum="DAA5DA580E77995B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJV3</accession>
    <name>A0A6I8UJV3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356682.3">
        <property type="nucleotide sequence ID" value="XM_001356646.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJV3"/>
    <dbReference type="FunCoup" id="A0A6I8UJV3">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816872"/>
    <dbReference type="InParanoid" id="A0A6I8UJV3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026148770" description="BPTI/Kunitz inhibitor domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="2">
        <location>
            <begin position="33"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356682.3"/>
        </source>
    </evidence>
    <sequence length="99" mass="11191" checksum="6240FD58D85F800C" modified="2020-08-12" version="1">MKANSQLRITVWLCVILTMIQDQPIEARSRDLCTVQPSTNGLCVPSTLGIYFDVETQHCRFLGCGNKRLFASLEDCEKICNSIKHVKWRSRSGGNETTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULX5</accession>
    <name>A0A6I8ULX5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">INO80 complex subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4803332</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357513.3">
        <property type="nucleotide sequence ID" value="XM_001357477.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULX5"/>
    <dbReference type="FunCoup" id="A0A6I8ULX5">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="GeneID" id="4803332"/>
    <dbReference type="KEGG" id="dpo:4803332"/>
    <dbReference type="InParanoid" id="A0A6I8ULX5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070355">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23021">
        <property type="entry name" value="zf-HIT_IN80B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF12">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="189"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357513.3"/>
        </source>
    </evidence>
    <sequence length="325" mass="37409" checksum="A6AADD9319381ADB" modified="2020-08-12" version="1">MFMIFESRNYLEIMGKVEDRRSKRSKRQKPARFASPTDSSVATNNEPPPNLAQEIQKSFPPVEHSIKREAQTRNVNIIDFVNNRTRPANTKKTVQASTSPSNAMKKIKRRRNSDTSSDEERWLNAIETGKLDDVDEELKKIKDPKLMTARQRAMYERTQDADTNMIGFVEELIALPSGYKEKEKPQTAEEIQKAALKSQKRKQQADERREKDKQKTMDRLLKKQESSKQRSGVRNKQTQKPSHPKLTYMNTIEGSYILVPPGHEFPMEAKVSRPPPPAQFCCISDCKNRKAYSCSKTNVPLCSLVCYRKHLTAAKLEQKNPNRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM22</accession>
    <name>A0A6I8UM22_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MICOS complex subunit MIC19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Chchd3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357579.3">
        <property type="nucleotide sequence ID" value="XM_001357542.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM22"/>
    <dbReference type="FunCoup" id="A0A6I8UM22">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800334"/>
    <dbReference type="InParanoid" id="A0A6I8UM22"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012471">
        <property type="entry name" value="DUF1690"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052632">
        <property type="entry name" value="MICOS_subunit_Mic19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21588">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21588:SF18">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07956">
        <property type="entry name" value="DUF1690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357579.3"/>
        </source>
    </evidence>
    <sequence length="209" mass="22731" checksum="0BF0D1462D55049A" modified="2020-08-12" version="1">MGARQSQSREPRSVSMENPTPAGVIDISDDVVKRLKAGISQQARQQAAVAEESKPAAKAPSVPAAVLPAPASAVRAPSSIYVQGGGQTISAADVQRQMNQELKQNDEIWRQRLINLEENLKKTNTILESEYANAVENVHKRFVSTAAAQKLPPCQDLKAQLLACYRAHPGETLQCMDEVAQFRQCVDLHRIKKLDDEPEAPKAQAAKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM43</accession>
    <name>A0A6I8UM43_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein DDB_G0287625</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">eIF4H2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357599.4">
        <property type="nucleotide sequence ID" value="XM_001357562.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM43"/>
    <dbReference type="KEGG" id="dpo:4800308"/>
    <dbReference type="InParanoid" id="A0A6I8UM43"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000414">
        <property type="entry name" value="Eukaryotic translation initiation factor 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="108"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="243"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="243"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="322"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001357599.4"/>
        </source>
    </evidence>
    <sequence length="471" mass="52331" checksum="832C862318190590" modified="2020-08-12" version="1">MANRSFDSGKNPRDREYDREFNRDYNRDNDRGGNARGNYRGMNRDNNRGGRDNRDNNRDDRNRQDRGGCRDSRDFRNNDRDRDRDRNGDRDRNRDRNSQKPLPTEPPFSAYVGNLPQGLVQGDVMKIFSDFQVKNVRLIKDRETDEFKGYGYVEFETVEQLERAIACNGRIKLDNFSAPLRIDIADHRRHTAEGGLGRGGGGGGGGGGGPGHGQSGSRNYYQRRNYRRDDSVGSHQIVRPIAATNSPGQQPFSHSSPTQSSISLRNAQRVGDNSNTSNNNRSIGYGREGGNRYQREDNRQRSRNNSIGFLEPRQSASSIQSRNRNFNRWSNNGNNEGGGGGGGGGRSPDRQRYNSCSNASGSSNVNGSGNYTNFVQNRNRDRRGHYNPNGGGNGSLRGNSPGSTSVRSANRIDDDNRPKLLLKPRTVTDPINALANTEQASKIFGKAKPRPDQTTPTCSPRQEPNGAPSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMQ7</accession>
    <name>A0A6I8UMQ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein C12orf4 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4800637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357874.3">
        <property type="nucleotide sequence ID" value="XM_001357837.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMQ7"/>
    <dbReference type="FunCoup" id="A0A6I8UMQ7">
        <property type="interactions" value="2608"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800637"/>
    <dbReference type="InParanoid" id="A0A6I8UMQ7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019311">
        <property type="entry name" value="Fy-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16525">
        <property type="entry name" value="PROTEIN C12ORF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16525:SF0">
        <property type="entry name" value="PROTEIN C12ORF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10154">
        <property type="entry name" value="Fy-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357874.3"/>
        </source>
    </evidence>
    <sequence length="538" mass="60879" checksum="9C63750AF3FA6A71" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNL0</accession>
    <name>A0A6I8UNL0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358366.3">
        <property type="nucleotide sequence ID" value="XM_001358329.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNL0"/>
    <dbReference type="KEGG" id="dpo:4801243"/>
    <dbReference type="InParanoid" id="A0A6I8UNL0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051481">
        <property type="entry name" value="BTB-POZ/Galectin-3-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410:SF23">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410">
        <property type="entry name" value="HL07962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="72"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358366.3"/>
        </source>
    </evidence>
    <sequence length="297" mass="33777" checksum="595A708F8CE3761C" modified="2020-08-12" version="1">MEQNQRSSLQSNDEKNNVDSSGPSNNPPIGGKVDFYAAMNNSNQPPPSMDDEMIMNSSRIRRIEYLRRAIDSDCQIHVQNMSVTPDGQAGSVCYKTFECHRIFLATSSEKMETDIFKNPNWNGVLQMNGVSPESVQIFLEYIYTFEVDSPLVGLNIIGDIFILSNAYQMPELMAAFFEKVKEQDWPLVDVIPAFDLAFRQCGMAELEQMCLDIIIPNGMQLVNEPSLMKIQLYALNYLVQLWMATETVPRERLVQLLQDYQAANGLNFNSVVRFPHFTKIIKHFPDILLDAEGSIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNN6</accession>
    <name>A0A6I8UNN6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2428 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358305.3">
        <property type="nucleotide sequence ID" value="XM_001358268.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNN6"/>
    <dbReference type="FunCoup" id="A0A6I8UNN6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801171"/>
    <dbReference type="InParanoid" id="A0A6I8UNN6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2428" evidence="2">
        <location>
            <begin position="640"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358305.3"/>
        </source>
    </evidence>
    <sequence length="1136" mass="129222" checksum="93A69635A3B78E88" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPN2</accession>
    <name>A0A6I8UPN2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kunitz-type serine protease inhibitor Hg1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358859.3">
        <property type="nucleotide sequence ID" value="XM_001358822.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPN2"/>
    <dbReference type="KEGG" id="dpo:4801826"/>
    <dbReference type="InParanoid" id="A0A6I8UPN2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0064437">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026323072" evidence="1">
        <location>
            <begin position="27"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="2">
        <location>
            <begin position="48"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358859.3"/>
        </source>
    </evidence>
    <sequence length="105" mass="11719" checksum="CFE7533B2B7C6BC6" modified="2020-08-12" version="1">MKLKINLTFLWATLVGLLSVILLTQTAQIEASSAGTDAVCKELNNVNCYVGRNEGNFCNSKDQTKAVTRWYYDKGICRSFTYKGCNGNRNRFCTQESCDVRCGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ46</accession>
    <name>A0A6I8UQ46_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pheromone-binding protein-related protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Obp83b</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PBP/GOBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358939.1">
        <property type="nucleotide sequence ID" value="XM_001358902.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ46"/>
    <dbReference type="FunCoup" id="A0A6I8UQ46">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="GeneID" id="4801921"/>
    <dbReference type="KEGG" id="dpo:4801921"/>
    <dbReference type="InParanoid" id="A0A6I8UQ46"/>
    <dbReference type="OMA" id="ASKGCIH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071052">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.20:FF:000001">
        <property type="entry name" value="General odorant-binding protein lush"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857:SF45">
        <property type="entry name" value="GENERAL ODORANT-BINDING PROTEIN 83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11857">
        <property type="entry name" value="ODORANT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00485">
        <property type="entry name" value="MEALWORMBTLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00708">
        <property type="entry name" value="PhBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026128612" evidence="6">
        <location>
            <begin position="18"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358939.1"/>
        </source>
    </evidence>
    <sequence length="142" mass="16182" checksum="C9DFB9649B1211E4" modified="2020-08-12" version="1">MMKSVGLLMLLLGCVAAQGPRRDAEYPPPAILKLAQHFHDICAPKTGVTDAAIKEFSDGEIHEDENLKCYMNCLFHEFEVVDDNGDVHMEKLFNAVPSEKLRNVLMEASKDCIHPEGDTLCHKAWWFHQCWKKADPVHYFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQA9</accession>
    <name>A0A6I8UQA9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">superoxide dismutase</fullName>
            <ecNumber evidence="1">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Sodq</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001359193.3">
        <property type="nucleotide sequence ID" value="XM_001359156.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQA9"/>
    <dbReference type="FunCoup" id="A0A6I8UQA9">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802230"/>
    <dbReference type="InParanoid" id="A0A6I8UQA9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000010">
        <property type="entry name" value="Copper-zinc superoxide dismutase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026193661" description="superoxide dismutase" evidence="7">
        <location>
            <begin position="24"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="8">
        <location>
            <begin position="108"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359193.3"/>
        </source>
    </evidence>
    <sequence length="266" mass="27782" checksum="2A3EC181F10115A4" modified="2020-08-12" version="1">MLIKLLPLILIIGYSNIFVPGSAQNLLDRLRPVNVTRFDDGIKVVSGTKVKRYERLTVPLGGPVGIPGGAGLLGPLLPPQPAYLGYTYLVPQWQAGAKLVGDGEAAGVAGMITFQQLPYNSDIKVTINVTGLPPGKHALHIHTFGDVSDGCKSTGGQFPNNFLGNVDAKDDGSISAVFQSIYIQLFGFNGIVGRAIVIHSKAIDLNTALNAEVFSSSLQAMPNALAYQNEENSVGPPIACGVISLMSTAAAPASGSSAAAVEKREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USC1</accession>
    <name>A0A6I8USC1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS10m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">28S ribosomal protein S10, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mRpS10</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359889.2">
        <property type="nucleotide sequence ID" value="XM_001359852.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USC1"/>
    <dbReference type="SMR" id="A0A6I8USC1"/>
    <dbReference type="FunCoup" id="A0A6I8USC1">
        <property type="interactions" value="802"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803090"/>
    <dbReference type="InParanoid" id="A0A6I8USC1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000005">
        <property type="entry name" value="28S ribosomal protein S10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040055">
        <property type="entry name" value="Ribosomal_uS10m"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13334">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13334:SF4">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US10M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="8">
        <location>
            <begin position="46"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359889.2"/>
        </source>
    </evidence>
    <sequence length="162" mass="18603" checksum="86AE4AD5470EDC84" modified="2020-08-12" version="1">MLQVLKTLRWAQPVRALSSVTTSSAATNVTTTASVPEPDKLFSKLEIELRGVDPAVLKSYTWFATTAADHLGIEKGKCWSPRKADHERMTLLKSVHIYKKHRVQYEVRTHFRYMNFHKLTGSTLDTFLEYIERNLPEGVALQVSKTELQQMPEHLRQPPEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USJ2</accession>
    <name>A0A6I8USJ2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable ribosome biogenesis protein RLP24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RpL24-like</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biogenesis of the 60S ribosomal subunit. Ensures the docking of NOG1 to pre-60S particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associated with nucleolar and cytoplasmic pre-60S particles. At the end of biogenesis it dissociates from cytoplasmic pre-60S particles and is likely to be exchanged for its ribosomal homologue, RPL24.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359978.1">
        <property type="nucleotide sequence ID" value="XM_001359941.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USJ2"/>
    <dbReference type="SMR" id="A0A6I8USJ2"/>
    <dbReference type="FunCoup" id="A0A6I8USJ2">
        <property type="interactions" value="1774"/>
    </dbReference>
    <dbReference type="GeneID" id="4803195"/>
    <dbReference type="KEGG" id="dpo:4803195"/>
    <dbReference type="InParanoid" id="A0A6I8USJ2"/>
    <dbReference type="OMA" id="TCYFCSG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079842">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF8">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="TRASH" evidence="8">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359978.1"/>
        </source>
    </evidence>
    <sequence length="192" mass="22707" checksum="BD98AF246BCF97CE" modified="2020-08-12" version="1">MRIETCYFCSSKIYPGHGVNFVRNDCKIFKFCRGKCHKAFKRKKNPRKVGWTKAHRKTAGKELAIDPSFEFEKRRNVPVKYSRETWQKALEAIKKVTEIKQRRQDHFVMERLRKGREIEIQMDVKNVQRNMSLIRSPAAGLKERRAKEEAEEAALMDEEDLPEEKITYVDARELEKRLEEGMGIEDLEMLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTC2</accession>
    <name>A0A6I8UTC2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Nuclear pore glycoprotein p62</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Nup62</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin NSP1/NUP62 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360457.2">
        <property type="nucleotide sequence ID" value="XM_001360420.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTC2"/>
    <dbReference type="FunCoup" id="A0A6I8UTC2">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="GeneID" id="4803799"/>
    <dbReference type="KEGG" id="dpo:4803799"/>
    <dbReference type="InParanoid" id="A0A6I8UTC2"/>
    <dbReference type="OMA" id="DIFRAPY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079466">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000040">
        <property type="entry name" value="Nuclear pore glycoprotein p62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026010">
        <property type="entry name" value="NSP1/NUP62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007758">
        <property type="entry name" value="Nucleoporin_NSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084:SF0">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05064">
        <property type="entry name" value="Nsp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin NSP1-like C-terminal" evidence="11">
        <location>
            <begin position="189"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="196"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001360457.2"/>
        </source>
    </evidence>
    <sequence length="379" mass="39509" checksum="4C6ECA9E7D3ABFEC" modified="2020-08-12" version="1">MAFQLPTTTSAPAAAGSFSFGMNAQAPTAGAAAAPAAKPAFSFGGPAGTNIGGGDADSGKAQAPPPFSGFGLPGASTAPALSLSAPLGGSNTGVTSTAAPAAATAAAPTFGTGFMAQPTAAPTQATSAPNTGLIGGPAIGLTTTPKSTAAPTAAVVAPLTGVVAPAVGTGGAFANLTTETKTTDSSAVSNASQLSYNQLEEHINKWTLEFEEQEKIFTEQATQINAWDKLLISNNHKIIELNDAVKKVKNDQQVLDQELEFIATQHKELEESLAPLEKEFVALPRVDMERNQTFLMVENLDTQLKRMSEDLKEIIDNLNETSKGQDNTDPIIQIGKILNAHMSSLQWIESQSNNICKKLDDIGKIHDTQKRDVFRPPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTR1</accession>
    <name>A0A6I8UTR1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4804008</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360639.2">
        <property type="nucleotide sequence ID" value="XM_001360602.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTR1"/>
    <dbReference type="FunCoup" id="A0A6I8UTR1">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="ESTHER" id="drops-q290z8">
        <property type="family name" value="Acidic_Lipase"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804008"/>
    <dbReference type="InParanoid" id="A0A6I8UTR1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000021">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026216742" description="Lipase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="10">
        <location>
            <begin position="34"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001360639.2"/>
        </source>
    </evidence>
    <sequence length="398" mass="44648" checksum="8592E93195EE5829" modified="2020-08-12" version="1">MLVPQVFLGLLCIGLATALPADGGRVSSVTTLTLVRAQGYEIQEHTVQTSDGYILTMHRIPYSKNTGYDGARPVAFLMHGLLCSSSDWVLGGTHSGLAYLLSEAGYDVWMGNARGNTYSKKHATHTPLLQPFWNFEWHDIGIYDLPAMIDYVLYATGVDQLSYVGHSQGTTSFFVLSSMIPRFKSRIRSAHLLAPVAWMEHMESPLATVGGPLLGQPNAFVELFGSMEFLPNTQLMNLMGSLMCSDQAISQVICTNSLFLMGGWNSPYLNESMIPEIMATTPAGCSVNQIFHYLQEYNSGYFRRFDYGSTRNKKEYGSKAPPDYDVEGINVPTYLYYSDNDYFASLIDVDRLRYVMDPNSLKSAYRLPETKWNHLDFLWGLNVKEILYDRVINDIKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUG5</accession>
    <name>A0A6I8UUG5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tyrosinase</fullName>
            <ecNumber evidence="4">1.14.18.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PPO2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 L-dopa + O2 = 2 L-dopaquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:34287"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57504"/>
            <dbReference type="ChEBI" id="CHEBI:57924"/>
            <dbReference type="EC" id="1.14.18.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosine + O2 = L-dopaquinone + H2O</text>
            <dbReference type="Rhea" id="RHEA:18117"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57924"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="EC" id="1.14.18.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tyrosinase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.18.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001361108.2">
        <property type="nucleotide sequence ID" value="XM_001361071.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUG5"/>
    <dbReference type="FunCoup" id="A0A6I8UUG5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="4804567"/>
    <dbReference type="KEGG" id="dpo:4804567"/>
    <dbReference type="InParanoid" id="A0A6I8UUG5"/>
    <dbReference type="OMA" id="YYMHEQI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080875">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004097">
        <property type="term" value="F:catechol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004503">
        <property type="term" value="F:tyrosinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042417">
        <property type="term" value="P:dopamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042438">
        <property type="term" value="P:melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035011">
        <property type="term" value="P:melanotic encapsulation of foreign target"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1280.10:FF:000004">
        <property type="entry name" value="Hemocyanin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1520:FF:000001">
        <property type="entry name" value="Hemocyanin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1370.10:FF:000001">
        <property type="entry name" value="Phenoloxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1520">
        <property type="entry name" value="Hemocyanin, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.10">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013788">
        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000896">
        <property type="entry name" value="Hemocyanin/hexamerin_mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005203">
        <property type="entry name" value="Hemocyanin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037020">
        <property type="entry name" value="Hemocyanin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005204">
        <property type="entry name" value="Hemocyanin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036697">
        <property type="entry name" value="Hemocyanin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511">
        <property type="entry name" value="LARVAL STORAGE PROTEIN/PHENOLOXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511:SF4">
        <property type="entry name" value="PHENOLOXIDASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03723">
        <property type="entry name" value="Hemocyanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00372">
        <property type="entry name" value="Hemocyanin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03722">
        <property type="entry name" value="Hemocyanin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00187">
        <property type="entry name" value="HAEMOCYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48050">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00209">
        <property type="entry name" value="HEMOCYANIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00210">
        <property type="entry name" value="HEMOCYANIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0470">Melanin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="15">
        <location>
            <begin position="398"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001361108.2"/>
        </source>
    </evidence>
    <sequence length="685" mass="79319" checksum="FB128516664FFEE8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVP5</accession>
    <name>A0A6I8UVP5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4805171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361618.2">
        <property type="nucleotide sequence ID" value="XM_001361581.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVP5"/>
    <dbReference type="FunCoup" id="A0A6I8UVP5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="4805171"/>
    <dbReference type="KEGG" id="dpo:4805171"/>
    <dbReference type="InParanoid" id="A0A6I8UVP5"/>
    <dbReference type="OMA" id="PPTWSGE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072261">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001361618.2"/>
        </source>
    </evidence>
    <sequence length="135" mass="15716" checksum="FF2B3C0C5F9A6492" modified="2020-08-12" version="1">MSLRRKEDYIPIKCEGWDGKFVYQDGAVNNVSKVRRHNQNVTNTNNFYGCNTEPIVLPTAWDGTFVKSGETRIKPERNRSDDQDYYDYNSDPTVLPTTWSGEFVTDPNDVRIKPERNFSDARDYAEASRFKLVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVV1</accession>
    <name>A0A6I8UVV1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ceramide glucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">GlcT</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N-(9Z-octadecenoyl)-sphing-4-enine + UDP-alpha-D-xylose = beta-D-xylosyl-(1&lt;-&gt;1')-N-(9Z-octadecenoyl)-sphing-4-enine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:77996"/>
            <dbReference type="ChEBI" id="CHEBI:189081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70248"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>UDP-alpha-D-xylose + an N-acylsphing-4-enine = a beta-D-xylosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189068"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:70244"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001361701.2">
        <property type="nucleotide sequence ID" value="XM_001361664.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVV1"/>
    <dbReference type="FunCoup" id="A0A6I8UVV1">
        <property type="interactions" value="648"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805276"/>
    <dbReference type="InParanoid" id="A0A6I8UVV1"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02520">
        <property type="entry name" value="Glucosylceramide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000041">
        <property type="entry name" value="UDP-glucose ceramide glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13506">
        <property type="entry name" value="Glyco_transf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001361701.2"/>
        </source>
    </evidence>
    <sequence length="439" mass="49442" checksum="921350542EF51ED1" modified="2020-08-12" version="1">MSHLPVPLYGFAAFFMIFWFGTWMVHLIAICYGKYKLHKKSCKLPPESSPLPGVSILKPLMGVDPNLQHNLETFFTMDYPLYELLFCVEDKDDPAIKLVEGLLEKYPRIDARLFVGGSDVGVNPKINNIHPGYMAAKFDFVMISDSGIKMKNDTLLDMVQNMSEKHALVHQMPFTCDREGFAAIFEKVFFGTVQSRIYLSADVLGINCHTGMSCLLRKAVIDQLGGLRAFGCYLAEDFFIAKGVTKLGWKMRISNQPALQNSGLCDIGSFQARLIRWAKLRVAMVPTTILLEPLSECMILGAFAAWSASVLFNWDPLVFYMVHILCWLLSDWLLLSIVQHGSMPFHKFEFVIGWLFRELTGPYLFLHALWDPAIRWRTRTFKLHWGGMAYELNTPADCQTAPLQPQPAPTLPTDSGQAVAEVTPAATFCTGAKHRLLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVW6</accession>
    <name>A0A6I8UVW6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dynein regulatory complex protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4804972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361447.3">
        <property type="nucleotide sequence ID" value="XM_001361410.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVW6"/>
    <dbReference type="FunCoup" id="A0A6I8UVW6">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="GeneID" id="4804972"/>
    <dbReference type="KEGG" id="dpo:4804972"/>
    <dbReference type="InParanoid" id="A0A6I8UVW6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070779">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46763">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46763:SF1">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361447.3"/>
        </source>
    </evidence>
    <sequence length="186" mass="21378" checksum="D858F3E601D582FC" modified="2020-08-12" version="1">MAKLLIEKLQSNVGEIESNFQQSQGKYQVCNMDMDMNNDLEKRISDAFCVFDHHGDKFIDVREVGTVLRLLGCVPTEEEVNEVISATESEETSGEVHLTKFLPHVSQLLMERKMEPAPPEKILQAFKILDPENKGYLAKETFGKLMMEEGEPFTQEEMDEMWPVAIDPISGHIPYEFYLNQLMVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWZ8</accession>
    <name>A0A6I8UWZ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132311.2">
        <property type="nucleotide sequence ID" value="XM_002132275.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWZ8"/>
    <dbReference type="KEGG" id="dpo:6903432"/>
    <dbReference type="InParanoid" id="A0A6I8UWZ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UWZ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026134992" evidence="1">
        <location>
            <begin position="23"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132311.2"/>
        </source>
    </evidence>
    <sequence length="181" mass="21337" checksum="E6335CE51822199A" modified="2020-08-12" version="1">MNTLFWIIISSSVISLMKPVISGEMSVYFDEFVIKYWMPDLFEKVECELFHIDNRSYVNGGFMLKRNVSEMSVRTAMDFWKPNNQKMKIYDVRLDACLLLRTAHKNRLFNIYVKSFKKHTKANLICPFKANYSYTFTNWQVETQDLPSFIPVGTFRTITEYFTQQQLGLRIVTSGKFTAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX92</accession>
    <name>A0A6I8UX92_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Ionotropic receptor 21a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Ir21a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132469.2">
        <property type="nucleotide sequence ID" value="XM_002132433.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX92"/>
    <dbReference type="FunCoup" id="A0A6I8UX92">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903287"/>
    <dbReference type="InParanoid" id="A0A6I8UX92"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050907">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF24">
        <property type="entry name" value="IONOTROPIC RECEPTOR 60A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026155864" evidence="11">
        <location>
            <begin position="22"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="681"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="12">
        <location>
            <begin position="406"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="722"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="849"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="858"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002132469.2"/>
        </source>
    </evidence>
    <sequence length="868" mass="97697" checksum="107EAF52759CE5CC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY05</accession>
    <name>A0A6I8UY05_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC6902570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <location>
            <begin position="459"/>
            <end position="498"/>
        </location>
    </feature>
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        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133098.2"/>
        </source>
    </evidence>
    <sequence length="498" mass="58185" checksum="7D3E44279FF60D88" modified="2020-08-12" version="1">MSILNIISEGSIDSSASVQYGGVQGSGVAQQDDKLDEDLEELYKRIMPIDTDYDPALCRDQFLKNLFEDLGIFDPNEVDNDPLLEVGSVSSAYMLAGKRGLSQPANWTLDSFVRKLKRKETLKALIPQTIPIQIGDKVLQLASKTDQYDALLESMQRSSLFFMFRRTCYNLHILQNASPETGELHVQDLPHNLFSWSLEDFFRRRELKDFVYLDVLVRDHTANTLDWAKYRVDVQETERFYGYFYSEIFNGAGYRKLLDLIWEQKQMLIIDKALQQDLEKSKASLDTTNFKARRYSQAWWQGYKSNMVEELQQSCMEATVTIPIECRYLCAYYETQVELQLMKDNVRTRQLQKELEDVRRSIQQGDDTSQASLNTYYAIIENYRNRYSELSDKYDRDMEHWEDEILQTNFRLSKARDDMKEAIDHVIFMRRRIAEVQALIEAERQEEIDRVANEQVERQKAAAKKANAKQQKKKQQAKARKVRAQKRQSQLSQSGKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYT5</accession>
    <name>A0A6I8UYT5_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_002133594.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:6902099"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Calponin-like domain"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="8"/>
            <end position="109"/>
        </location>
    </feature>
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        <location>
            <begin position="159"/>
            <end position="215"/>
        </location>
    </feature>
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        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133630.2"/>
        </source>
    </evidence>
    <sequence length="258" mass="30506" checksum="189677508993FC9A" modified="2020-08-12" version="1">MPGTHGHPTNPKILLKWINIMLRSDYRSMSELNNGAAWCRLFYCIYPDSFNVEAVVDVGPPAVLQRNYDILMRTLNRLGIPRQFCVQKLMKGNTTDTIELVHFFMDLYVHKKLAQNRQRPQPQLQRGPLKEFLGWLKRLIVGTSMEDDPEAYLLLFEEMNDPKPQRRSQPTQTLSPEMNHRLSGGQKKEPAQQPQQQQQQQPEDLQGHSDQRRKDHDEIRPLIDACHQMLQFQNDMEERWLEYRRARLARQKTNQRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ04</accession>
    <name>A0A6I8UZ04_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein beat-Ic isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">beat-Ic</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133194.2">
        <property type="nucleotide sequence ID" value="XM_002133158.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235653.1">
        <property type="nucleotide sequence ID" value="XM_033379762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ04"/>
    <dbReference type="KEGG" id="dpo:6902678"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ04">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000437">
        <property type="entry name" value="Beat-IIIc, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002320">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261">
        <property type="entry name" value="BEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21261:SF8">
        <property type="entry name" value="BEATEN PATH IA, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="65"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133194.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235653.1"/>
        </source>
    </evidence>
    <sequence length="583" mass="63877" checksum="FAEEFA1C7311D1E7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ06</accession>
    <name>A0A6I8UZ06_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein PRY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PRY</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133779.3">
        <property type="nucleotide sequence ID" value="XM_002133743.3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902252"/>
    <dbReference type="InParanoid" id="A0A6I8UZ06"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ06">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002859">
        <property type="entry name" value="PKD/REJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730:SF1">
        <property type="entry name" value="PLAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730">
        <property type="entry name" value="POLYCYSTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02010">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026006529" evidence="8">
        <location>
            <begin position="22"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1076"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1292"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1330"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="9">
        <location>
            <begin position="1121"/>
            <end position="1237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002133779.3"/>
        </source>
    </evidence>
    <sequence length="1362" mass="158205" checksum="E704ED2A794C59F2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ49</accession>
    <name>A0A6I8UZ49_DROPS</name>
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        <submittedName>
            <fullName evidence="3">ARL-6-interacting protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6901257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133958.3">
        <property type="nucleotide sequence ID" value="XM_002133922.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ49"/>
    <dbReference type="KEGG" id="dpo:6901257"/>
    <dbReference type="InParanoid" id="A0A6I8UZ49"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ49">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052114">
        <property type="entry name" value="ER_autophagy_membrane_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952:SF0">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952">
        <property type="entry name" value="ADP-RIBOSYLATION-LIKE FACTOR 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133958.3"/>
        </source>
    </evidence>
    <sequence length="205" mass="23461" checksum="AC1EC96587756AE2" modified="2020-08-12" version="1">MSKTPIELDTQRSTEMTASDIGNNAMVIKLKHDLERFQAPIVSAYGVLTWEKKYYPVVVLGGISVTFFVLWYMDLSVITLMSLLALLTTISHYFLLWCLDLPDFILDFFQTKIDGLFWNALIWDSDNEAKFESVCGQMYNIKKGLAAWYQYLFKERKSTGLVIVKSLVLLAMAWIGSIVNNLLLMYLVTLLIAMWAGLQAKDVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZD4</accession>
    <name>A0A6I8UZD4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">LOW QUALITY PROTEIN: uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6901311</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134012.3">
        <property type="nucleotide sequence ID" value="XM_002133976.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UZD4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901311"/>
    <dbReference type="InParanoid" id="A0A6I8UZD4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF325">
        <property type="entry name" value="POTASSIUM CHANNEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="225"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="391"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="295"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="660"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="349"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="691"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="713"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002134012.3"/>
        </source>
    </evidence>
    <sequence length="752" mass="78823" checksum="BECD1EB352038E77" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1G9</accession>
    <name>A0A6I8V1G9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC6900180</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_002135519.2">
        <property type="nucleotide sequence ID" value="XM_002135483.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1G9"/>
    <dbReference type="KEGG" id="dpo:6900180"/>
    <dbReference type="InParanoid" id="A0A6I8V1G9"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="103"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002135519.2"/>
        </source>
    </evidence>
    <sequence length="229" mass="24445" checksum="0780D421DB6BD1DC" modified="2020-08-12" version="1">MESAPSSSGTGCNVPATGTADTEGHVSATNAEQNNGHRDSTTTQLVPEEEGAAASSSATSDSLTGSSLPRGNIADLSTDSPAAGGSSSGAADPERVDPSVYECNICFDTATDAVVTMCGHLFCWPCLHQWFLRRPLVKLCPVCKGTIDNDKVIPIYGRNAEHQVDPRNRIPARPAGQRREPMPARFGLRHPGDILMFFGTPTAENQDAQTLSKMFIYVALLCIAWLFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V311</accession>
    <name>A0A6I8V311_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protein abnormal spindle</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">asp</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002136959.2">
        <property type="nucleotide sequence ID" value="XM_002136923.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V311">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896752"/>
    <dbReference type="InParanoid" id="A0A6I8V311"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051295">
        <property type="term" value="P:establishment of meiotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21223">
        <property type="entry name" value="CH_ASPM_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000051">
        <property type="entry name" value="Abnormal spindle-like microcephaly-associated protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031549">
        <property type="entry name" value="ASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051185">
        <property type="entry name" value="ASPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15780">
        <property type="entry name" value="ASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="13">
        <location>
            <begin position="836"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="175"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="472"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002136959.2"/>
        </source>
    </evidence>
    <sequence length="1955" mass="230586" checksum="ADFB62ACDAF84DAC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3J0</accession>
    <name>A0A6I8V3J0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Modular serine protease-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6897854</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137957.4">
        <property type="nucleotide sequence ID" value="XM_002137921.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3J0"/>
    <dbReference type="KEGG" id="dpo:6897854"/>
    <dbReference type="InParanoid" id="A0A6I8V3J0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270:SF63">
        <property type="entry name" value="TERRIBLY REDUCED OPTIC LOBES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0768">Sushi</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026327528" evidence="10">
        <location>
            <begin position="17"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="287"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137957.4"/>
        </source>
    </evidence>
    <sequence length="547" mass="61059" checksum="75B8303DAF4639A2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3L0</accession>
    <name>A0A6I8V3L0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6897747</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137852.1">
        <property type="nucleotide sequence ID" value="XM_002137816.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3L0"/>
    <dbReference type="FunCoup" id="A0A6I8V3L0">
        <property type="interactions" value="260"/>
    </dbReference>
    <dbReference type="GeneID" id="6897747"/>
    <dbReference type="KEGG" id="dpo:6897747"/>
    <dbReference type="InParanoid" id="A0A6I8V3L0"/>
    <dbReference type="OMA" id="FWAPIVK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247672">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002137852.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="16462" checksum="D236683CFE84A891" modified="2020-08-12" version="1">MPPKPPSAAALASVGKRLGLHSKAYNGLIGVCDKFVPPKMRPLWMHPAGPKTIFFWAPLVKWGLVIAGLSDLTRPADTISPNGCLALGATNLIWTRYALVIIPKNYSLFAVNLFVSLTQLFQLGRAYNYKWEQTKLEKVTSEKPALLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3W8</accession>
    <name>A0A6I8V3W8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6897984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138083.3">
        <property type="nucleotide sequence ID" value="XM_002138047.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3W8"/>
    <dbReference type="KEGG" id="dpo:6897984"/>
    <dbReference type="InParanoid" id="A0A6I8V3W8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002138083.3"/>
        </source>
    </evidence>
    <sequence length="207" mass="23190" checksum="4D0180D653424206" modified="2020-08-12" version="1">MTPSEKKQENMNADVDMDAEVDMDAEVDMDVEVDMDAEVDMDADMEFLDSAIDLVFDADLTVEPQVRKQLLELVYARTHRLLMQAHENRNDRHSDDITLADWEKVPKVQMKKRSKLLSTDEKNDIMANQKSDMPMPSTTGPCSGLMLPPWRHCKMGPTAILKAMQAPQKIATAARSNGASCQGYFPVKGVPRFPAAKALPMGAFNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4U4</accession>
    <name>A0A6I8V4U4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">J domain-containing protein CG6693-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138671.2">
        <property type="nucleotide sequence ID" value="XM_002138635.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4U4"/>
    <dbReference type="KEGG" id="dpo:6898666"/>
    <dbReference type="InParanoid" id="A0A6I8V4U4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056453">
        <property type="entry name" value="HTH_DNAJC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23302">
        <property type="entry name" value="HTH_DNAJC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="15"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138671.2"/>
        </source>
    </evidence>
    <sequence length="217" mass="24940" checksum="79ED6F1660D56830" modified="2020-08-12" version="1">MSVCGDSWLMFGTRDFYKVLDVSRGAMPRAIRSAAYQLIMAEHPGQRPAKQRRKALQKCHLVYRIMSILTNDKQRALYDSPSEFPPESASCELDLAFATALDLCACLLGNRQDRSGVCELFQKHYKGSQLELADIKAAYTLGKGCMDRLFIELPLMTVRDEPRIRKIIKKLIKSNELPEYSKFINESVEKRRRRRLKFAVYEAKSKTEKNEDGLPQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4X1</accession>
    <name>A0A6I8V4X1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein telomere ends associated-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6898756</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138739.3">
        <property type="nucleotide sequence ID" value="XM_002138703.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V4X1">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898756"/>
    <dbReference type="InParanoid" id="A0A6I8V4X1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V4X1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054730">
        <property type="entry name" value="Tea_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057624">
        <property type="entry name" value="Tea_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054729">
        <property type="entry name" value="Tea_mid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22884">
        <property type="entry name" value="Tea_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24236">
        <property type="entry name" value="Tea_helical"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22889">
        <property type="entry name" value="Tea_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere ends associated alpha-helical" evidence="4">
        <location>
            <begin position="15"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Telomere ends associated alpha-helical" evidence="4">
        <location>
            <begin position="239"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Telomere ends associated alpha-helical" evidence="4">
        <location>
            <begin position="349"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Telomere ends associated alpha-helical" evidence="4">
        <location>
            <begin position="472"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Telomere ends associated alpha-helical" evidence="4">
        <location>
            <begin position="716"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="Telomere ends associated middle" evidence="3">
        <location>
            <begin position="1017"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="domain" description="Telomere ends associated C-terminal" evidence="2">
        <location>
            <begin position="1508"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="690"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="890"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="937"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="980"/>
            <end position="994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002138739.3"/>
        </source>
    </evidence>
    <sequence length="1672" mass="189771" checksum="7CD68E54BF8DB8A7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V608</accession>
    <name>A0A6I8V608_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6898827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138800.2">
        <property type="nucleotide sequence ID" value="XM_002138764.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V608"/>
    <dbReference type="FunCoup" id="A0A6I8V608">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898827"/>
    <dbReference type="InParanoid" id="A0A6I8V608"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004210">
        <property type="entry name" value="BESS_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505:SF8">
        <property type="entry name" value="DPT-YFP REPRESSOR BY OVEREXPRESSION, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505">
        <property type="entry name" value="MADF DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51031">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="2">
        <location>
            <begin position="23"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="BESS" evidence="3">
        <location>
            <begin position="209"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002138800.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="29807" checksum="532DCD5D10395F6A" modified="2020-08-12" version="1">MTSQAVATTGGLPFRFTDERTIKFVELYGREPCLWNKRPYLRGARNAAYKRIMAGINANIDRGENGLSIQGVKIKIKNLRTGYHQELKKIKSNPRRYRPKIPWFAPLHKFLAEFVDTSEHDTRDVPQLKRLSVRLHRLKPIKLQEMVKLEPEEIVTTEPDITTTPSLFTVLPVTMQTEQPPTPPPPLPKVPEVSIMPSSHTNRSEIRGEDEFTFFGLSVAAQLRNMPISNSMIMQSKIQYILSMERRKINGHAGDMNIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V924</accession>
    <name>A0A6I8V924_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Single domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC26532189</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015036349.2">
        <property type="nucleotide sequence ID" value="XM_015180863.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V924"/>
    <dbReference type="KEGG" id="dpo:26532189"/>
    <dbReference type="InParanoid" id="A0A6I8V924"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271176">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029277">
        <property type="entry name" value="SVWC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053308">
        <property type="entry name" value="Vago-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957">
        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957:SF1">
        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15430">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01318">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026361064" description="Single domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Single" evidence="4">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015036349.2"/>
        </source>
    </evidence>
    <sequence length="102" mass="11085" checksum="16BF519BF2BEA5DC" modified="2020-08-12" version="1">MHFAGILLVLSALFLAGTEADSMQAVFSDPEHPGKCVIDGLILSDGQWIRHPKMCALLWCGRNSIGGIHICGKVSPPPGSRFGEYTDPKADYPACCRREIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9F6</accession>
    <name>A0A6I8V9F6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4817366</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_015035993.2">
        <property type="nucleotide sequence ID" value="XM_015180507.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9F6"/>
    <dbReference type="FunCoup" id="A0A6I8V9F6">
        <property type="interactions" value="1075"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817366"/>
    <dbReference type="InParanoid" id="A0A6I8V9F6"/>
    <dbReference type="OMA" id="FHEVMET"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076800">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V9F6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000067">
        <property type="entry name" value="Dosage compensation regulator-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000145">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002204">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF136">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX35-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="57"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="246"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015035993.2"/>
        </source>
    </evidence>
    <sequence length="680" mass="76348" checksum="C5C1A872DFD81A2C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAU0</accession>
    <name>A0A6I8VAU0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Trypsin-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">LOC26533140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_015181331.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036818.2">
        <property type="nucleotide sequence ID" value="XM_015181332.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAU0"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634494" evidence="4">
        <location>
            <begin position="24"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="93"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015036817.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015036818.2"/>
        </source>
    </evidence>
    <sequence length="343" mass="38464" checksum="3A1FA5D919FAB31F" modified="2020-08-12" version="1">MLFKLVIWLILVVCVVERNPAEANEYYTCDSLSEKCVLFHACIHAKHKGYFKICAINSICCRVPKKPKADTRSSRACLSYASNTTFCPRHLFISHSQESYRNELQYMAHIGLTNPRAWICGGTLIHSKFVLTAAHCVILERAVGTEEPGFLVRLGGHNSTDGAIYEVAEKIPHPDYKEEDGTKNDIALLKLTKTVVFNDQVKPACLPTTSGQEHAKMTVSGWGLYSSYEPNKPAPQLRKADVTQFKYAECNEENEVLQEMKICAGGENDASDSCQGDSGGPLAIWHPEWGSCLGQVFGIVSEGSFCDTKSPRTIYTRVFYYLQWIEDIVWKTREETLSAAEWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDI5</accession>
    <name>A0A6I8VDI5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Ras guanine nucleotide exchange factor B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC6896949</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137134.2">
        <property type="nucleotide sequence ID" value="XM_002137098.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038874.2">
        <property type="nucleotide sequence ID" value="XM_015183388.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDI5"/>
    <dbReference type="KEGG" id="dpo:6896949"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="291"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="406"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137134.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015038874.2"/>
        </source>
    </evidence>
    <sequence length="597" mass="64635" checksum="98DCB8D9575E5A37" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDX9</accession>
    <name>A0A6I8VDX9_DROPS</name>
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        <submittedName>
            <fullName evidence="8">Sodium-independent sulfate anion transporter isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4800262</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015038742.2">
        <property type="nucleotide sequence ID" value="XM_015183256.2"/>
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    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="456"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="6">
        <location>
            <begin position="80"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015038742.2"/>
        </source>
    </evidence>
    <sequence length="625" mass="68156" checksum="E17D9B03B54D59B2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHH3</accession>
    <name>A0A6I8VHH3_DROPS</name>
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        <submittedName>
            <fullName evidence="17">Calpain-D isoform X1</fullName>
        </submittedName>
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        <name evidence="17" type="primary">sol</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
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        <property type="nucleotide sequence ID" value="XM_015185494.2"/>
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        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VHH3"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calpain 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000022">
        <property type="entry name" value="calpain-D isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
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        <property type="entry name" value="CALPAIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="718"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="758"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="931"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="domain" description="Calpain catalytic" evidence="15">
        <location>
            <begin position="1018"/>
            <end position="1325"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="190"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="349"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="429"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="524"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="872"/>
            <end position="906"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="429"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="535"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="546"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="578"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="873"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="1083"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="1249"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="1269"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015040980.2"/>
        </source>
    </evidence>
    <sequence length="1598" mass="176924" checksum="9EA81E68B45ECA55" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHW6</accession>
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        <recommendedName>
            <fullName evidence="4">Multivesicular body subunit 12A</fullName>
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        <alternativeName>
            <fullName evidence="12">ESCRT-I complex subunit MVB12A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Protein FAM125A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Mvb12</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MVB12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041711.2">
        <property type="nucleotide sequence ID" value="XM_015186225.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VHW6"/>
    <dbReference type="FunCoup" id="A0A6I8VHW6">
        <property type="interactions" value="355"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815087"/>
    <dbReference type="InParanoid" id="A0A6I8VHW6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032510">
        <property type="term" value="P:endosome to lysosome transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032801">
        <property type="term" value="P:receptor catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042058">
        <property type="term" value="P:regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046755">
        <property type="term" value="P:viral budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019075">
        <property type="term" value="P:virus maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.100.10.50:FF:000002">
        <property type="entry name" value="Multivesicular body subunit 12B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023341">
        <property type="entry name" value="MABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040335">
        <property type="entry name" value="MVB12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018798">
        <property type="entry name" value="MVB12A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023340">
        <property type="entry name" value="UMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31612">
        <property type="entry name" value="MULTIVESICULAR BODY SUBUNIT 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31612:SF2">
        <property type="entry name" value="MULTIVESICULAR BODY SUBUNIT 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10240">
        <property type="entry name" value="DUF2464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51498">
        <property type="entry name" value="MABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51497">
        <property type="entry name" value="UMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MABP" evidence="15">
        <location>
            <begin position="61"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="UMA" evidence="14">
        <location>
            <begin position="307"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015041711.2"/>
        </source>
    </evidence>
    <sequence length="365" mass="39264" checksum="C86C59FC9962D555" modified="2020-08-12" version="1">MNKVGKHIQSGVSSLGGGSPVALSTASLLGSPVDTETGVAGPGAGNATNVFNSIMSFLPDNRPITSLHIVQDFERCPKNFSAIHRTYDQDADADLWRDYSIFGRQNTRYLCLSKSEGLPDYVVETLQVIADKTTPPKEYSLLSRTADSDQKAWRKRQIAYKLSKRGSVTQAVTDIIVCSKLKVAPDGFKLAGDINGVLICYKTGAIPVRLPPPVPAGAAAATAGGGTCEVEQALNRLNLQGQRHSRGPLPQPPTAEHHDYEEIQANYQINSPQRPAPPRPAGSSAVVVGGGRAAYGGTGTLGTYTELEGVPFVMDVRLQRNQPGTDLPILPELSTLLRSLDYDFQLERQVLCTMKSSASKNPFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIS2</accession>
    <accession>A0A0R3P1T3</accession>
    <name>A0A6I8VIS2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">LOC26534385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3">MV-25-SWS-2005</strain>
            <tissue evidence="2 3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042242.1">
        <property type="nucleotide sequence ID" value="XM_015186756.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239507.1">
        <property type="nucleotide sequence ID" value="XM_033383616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VIS2"/>
    <dbReference type="GeneID" id="26534385"/>
    <dbReference type="KEGG" id="dpo:26534385"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0273372">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015042242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239507.1"/>
        </source>
    </evidence>
    <sequence length="131" mass="14468" checksum="7FB5ABF98AAA06FE" modified="2020-08-12" version="1">MNFSHQRGPMYKPLFEIIPNIPERVDVDAEQLGLGYLNILPYFGKMIELGLCAKSGVKGKLCGKLEMLTPEGEIVLTQCSEILIFGALTGKKLLLGIVKVPRTHVLSVFWEAASDKEPSAENSRSRSHSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJA9</accession>
    <name>A0A6I8VJA9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein Ir7e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ir7e</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015042163.2">
        <property type="nucleotide sequence ID" value="XM_015186677.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VJA9"/>
    <dbReference type="FunCoup" id="A0A6I8VJA9">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26533428"/>
    <dbReference type="InParanoid" id="A0A6I8VJA9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056198">
        <property type="entry name" value="LBD_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF30">
        <property type="entry name" value="IONOTROPIC RECEPTOR 40A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24061">
        <property type="entry name" value="LBD_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="580"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ionotropic receptor ligand binding" evidence="9">
        <location>
            <begin position="16"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015042163.2"/>
        </source>
    </evidence>
    <sequence length="614" mass="71326" checksum="C03DFA6780D7DEF0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKB5</accession>
    <name>A0A6I8VKB5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Protein melted isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">melt</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MELT/VEPH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015042748.2">
        <property type="nucleotide sequence ID" value="XM_015187262.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042749.2">
        <property type="nucleotide sequence ID" value="XM_015187263.2"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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    <sequence length="997" mass="108309" checksum="22A3CEB8A0AD66C2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMM6</accession>
    <name>A0A6I8VMM6_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Doa</name>
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    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0043484">
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="CLK_kinases"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
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            <end position="2075"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="282"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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    <feature type="region of interest" description="Disordered" evidence="17">
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    <feature type="region of interest" description="Disordered" evidence="17">
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    <feature type="region of interest" description="Disordered" evidence="17">
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1472"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1509"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1521"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1586"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1639"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1655"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1674"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1684"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2088"/>
            <end position="2098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="2099"/>
            <end position="2108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1784"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033232282.1"/>
        </source>
    </evidence>
    <sequence length="2108" mass="232715" checksum="EA64EE4B9C6CC7F9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPA5</accession>
    <name>A0A6I8VPA5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Electron transfer flavoprotein subunit beta</fullName>
            <shortName evidence="8">Beta-ETF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12 13 14 15" type="primary">Etfb</name>
        <name evidence="11" type="synonym">LOC4801964</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11 12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12 13">MV-25-SWS-2005</strain>
            <strain evidence="10 11">MV2-25</strain>
            <tissue evidence="11 12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase. Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. ETFB binds an AMP molecule that probably has a purely structural role.</text>
    </comment>
    <comment type="function">
        <text evidence="8">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interacts with ACADM.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358978.1">
        <property type="nucleotide sequence ID" value="XM_001358941.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232702.1">
        <property type="nucleotide sequence ID" value="XM_033376811.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232703.1">
        <property type="nucleotide sequence ID" value="XM_033376812.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232704.1">
        <property type="nucleotide sequence ID" value="XM_033376813.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232705.1">
        <property type="nucleotide sequence ID" value="XM_033376814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPA5"/>
    <dbReference type="GeneID" id="4801964"/>
    <dbReference type="KEGG" id="dpo:4801964"/>
    <dbReference type="OMA" id="EINQPRI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080612">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000011">
        <property type="entry name" value="Electron transfer flavoprotein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="9">
        <location>
            <begin position="24"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033232702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033232703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033232704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033232705.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="27238" checksum="E93F101BB7699581" modified="2020-08-12" version="1">MARVLVGVKRVIDYAVKVRVKPDKTGVVTQGVKHSMNPFDEIAVEEAVKMKEKKLATEVIAVSVGPAQSAEVIRTAMAMGADRGVHVDVPQAEYELLQPIHISKILAKLALDEKADLVILGKQAIDDDANQTAQMTAAVLDWPQGTFCNKIEKTDAGLTITREIDGGLETIKTKIPAVLSADLRLNTPRYATLPNIMKAKKKPLKKVTAKDLGVDTTPRIEVISVEDPPVRQAGATVADVDALVAKLKEGGHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQE1</accession>
    <name>A0A6I8VQE1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="13">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-specific succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="13">A-SCS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Succinyl-CoA synthetase beta-A chain</fullName>
            <shortName evidence="13">SCS-betaA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">ScsbetaA</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19 20">MV-25-SWS-2005</strain>
            <strain evidence="18">MV2-25</strain>
            <tissue evidence="19 20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="11">GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.2.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the succinate/malate CoA ligase beta subunit family. ATP-specific subunit beta subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="13"/>
    <dbReference type="RefSeq" id="XP_033232948.1">
        <property type="nucleotide sequence ID" value="XM_033377057.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232949.1">
        <property type="nucleotide sequence ID" value="XM_033377058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQE1"/>
    <dbReference type="KEGG" id="dpo:6897672"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247705">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VQE1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000004">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03220">
        <property type="entry name" value="Succ_CoA_betaA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034723">
        <property type="entry name" value="Succ_CoA_betaA_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF1">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 13 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 13" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp fold succinyl-CoA synthetase-type" evidence="17">
        <location>
            <begin position="39"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-citrate synthase/succinyl-CoA ligase C-terminal" evidence="16">
        <location>
            <begin position="307"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="366"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="13">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="13">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033232948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033232949.1"/>
        </source>
    </evidence>
    <sequence length="475" mass="52100" checksum="F4D2033E407262B7" modified="2020-08-12" version="1">MASFLARTGGPLIESVRPKTVQKILSYAPVALQQLRNLNVQEHVSYTLLNESGIPTPRFAVAKNGKEASDIANKLKTDNLVLKAQVLAGGRGKGTFKNGLKGGVRVVYDPKTAEDISSKMIDQLLITKQTGAAGRICKKVMVAERKFPRREFYFAVMMERAFNGPVLIASKEGGVDIEEVAKENPDAIIYEPIDIARGLTEEQACKIVEKVGLGGDGADEHIKMLLNLYQLFVKKDALLVEINPYAEDAMSGCFFALDAKLRFDDNAEFRQKELFSMRDWTQEDPKEVEAAKYNLNYIALDGTIGCMVNGAGLAMATMDIIKLYGGEPANFLDVGGGATADAVKAAFKIITSDRKVLCLLVNIFGGIMRCDVIAEGIIAAAKDLNLNLPIVVRLQGTKVKEARELIRASGLKILARDDLDKAADLAVHLAQIVKLAREMKMDVNFEIPADQKGKGKKKECKEEKSKKEEEKCKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQZ5</accession>
    <name>A0A6I8VQZ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183529</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233476.1">
        <property type="nucleotide sequence ID" value="XM_033377585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQZ5"/>
    <dbReference type="KEGG" id="dpo:117183529"/>
    <dbReference type="InParanoid" id="A0A6I8VQZ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233476.1"/>
        </source>
    </evidence>
    <sequence length="61" mass="7116" checksum="4C67778A8440BB77" modified="2020-08-12" version="1">MCGFGFVAYGFDCCHRSIDFSLNPETEIKVKCKVLKGEELKRQNKQFVMRVPVCQLEWTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VRZ1</accession>
    <name>A0A6I8VRZ1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3',5'-cyclic-AMP phosphodiesterase</fullName>
            <ecNumber evidence="4">3.1.4.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Pde8</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.53" evidence="4"/>
    <dbReference type="RefSeq" id="XP_033233822.1">
        <property type="nucleotide sequence ID" value="XM_033377931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VRZ1"/>
    <dbReference type="FunCoup" id="A0A6I8VRZ1">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VRZ1"/>
    <dbReference type="UniPathway" id="UPA00762">
        <property type="reaction ID" value="UER00747"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000096">
        <property type="entry name" value="High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000002">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000555">
        <property type="entry name" value="Phosphodiesterase 8, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057304">
        <property type="entry name" value="PDE8-like_REC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033234855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033234856.1"/>
        </source>
    </evidence>
    <sequence length="833" mass="85639" checksum="33C8B2E981FC9364" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYA5</accession>
    <name>A0A6I8VYA5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Gastrula zinc finger protein XlCGF57.1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6903240</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236052.1">
        <property type="nucleotide sequence ID" value="XM_033380161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYA5"/>
    <dbReference type="KEGG" id="dpo:6903240"/>
    <dbReference type="InParanoid" id="A0A6I8VYA5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VYA5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000450">
        <property type="entry name" value="PR domain zinc finger protein 14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000670">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001465">
        <property type="entry name" value="Zinc finger protein 560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001343">
        <property type="entry name" value="Zinc finger protein 568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF23">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="14">
        <location>
            <begin position="28"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="419"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033236052.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="65610" checksum="274E0D789EBE9D34" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZI2</accession>
    <name>A0A6I8VZI2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236511.1">
        <property type="nucleotide sequence ID" value="XM_033380620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZI2"/>
    <dbReference type="InParanoid" id="A0A6I8VZI2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VZI2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236511.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="29774" checksum="B7B7EFB9500BAD54" modified="2020-08-12" version="1">MSYDKPQTFSSYNHDEEEEHRLIEMFKNTNFIAESPTMIRPSRSKFTAEGIVSTPCDDSSLRKRNLNAVLKLPDLKERTSMSSMEGLYELSQKLAVTSPRLTKFLKDPEQPEEITDPDMVRGLENTPQKREEQPEATVQQPKVKGRILLFKQHQELKLWKSIIRNPINRNWCEQVKNKLKNKTKKKKKKKDHLPARVKAVLDLKRKNYINLLRKPTGHGARKYLKRFVASKVLPWLLTFGIAFLVYVTLLRWTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAV4</accession>
    <name>A0A6I8WAV4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Vesicle transport protein GOT1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">LOC26533264</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10">MV-25-SWS-2005</strain>
            <tissue evidence="9 10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239689.1">
        <property type="nucleotide sequence ID" value="XM_033383798.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239691.1">
        <property type="nucleotide sequence ID" value="XM_033383800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WAV4"/>
    <dbReference type="KEGG" id="dpo:26533264"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF9">
        <property type="entry name" value="GOLGI TRANSPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033239689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033239691.1"/>
        </source>
    </evidence>
    <sequence length="140" mass="15233" checksum="89506D34BB21B5EF" modified="2020-08-12" version="1">MIEVTDLQKIGIGLAGFGISFLFLGMLLLFDKGLLAIGNILFISGLGCVIGVERTLRFFFQRHKVKGTTAFFGGIFIVLLGFPIIGMIVESYGFFALFSGFFPVAINFLGRVPVLGSIFNLPFMQKIVQKLGGDGNRTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDF9</accession>
    <name>A0A6I8WDF9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acylneuraminate cytidylyltransferase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Csas</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241461.1">
        <property type="nucleotide sequence ID" value="XM_033385570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDF9"/>
    <dbReference type="FunCoup" id="A0A6I8WDF9">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900890"/>
    <dbReference type="InParanoid" id="A0A6I8WDF9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WDF9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008781">
        <property type="term" value="F:N-acylneuraminate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02513">
        <property type="entry name" value="CMP-NeuAc_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050793">
        <property type="entry name" value="CMP-NeuNAc_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21485">
        <property type="entry name" value="HAD SUPERFAMILY MEMBERS CMAS AND KDSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21485:SF3">
        <property type="entry name" value="N-ACYLNEURAMINATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033241461.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="29077" checksum="D44F18D9EA6CDAFD" modified="2020-08-12" version="1">MVLGSKCLHKYPVTKSHIDRKTKKLYVLAKCASVHSSKSEIHAIILARGGSKGIKYKNLVKIEGLSLLARTIITIQNSSCFDHIWVSTDDKRISIEANKYGAIVHNRPSEIARDETSSLDAIKEFLDVHKAIHNFSLFQCTSVFLKEKYINEAALAFKNHDCVFAAMRSHYLRWEFVDNLLVPVGFNSKARPRRQDWKGDIVETGMFYFSKRRLVESGFLQNNRCSIVEIDQKDGLEIDSPIDLTIARCILNNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDS2</accession>
    <name>A0A6I8WDS2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117185553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241581.1">
        <property type="nucleotide sequence ID" value="XM_033385690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDS2"/>
    <dbReference type="KEGG" id="dpo:117185553"/>
    <dbReference type="InParanoid" id="A0A6I8WDS2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009882">
        <property type="entry name" value="Gypsy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07253">
        <property type="entry name" value="Gypsy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241581.1"/>
        </source>
    </evidence>
    <sequence length="632" mass="73942" checksum="75536D211DFEBCC9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEM9</accession>
    <name>A0A6I8WEM9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hemotin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hemo</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241881.1">
        <property type="nucleotide sequence ID" value="XM_033385990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEM9"/>
    <dbReference type="KEGG" id="dpo:13036497"/>
    <dbReference type="InParanoid" id="A0A6I8WEM9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047851">
        <property type="entry name" value="Hemotin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21944">
        <property type="entry name" value="Hemotin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033241881.1"/>
        </source>
    </evidence>
    <sequence length="90" mass="10499" checksum="60C2AD469BD4224C" modified="2020-08-12" version="1">MDWSMKSHLLAYYYQCPNISPWCLILFGAIVAVTLVCYSCHCYQFIRDRRRLRSQNRQLPQPLVRLSVSPGCPHIINTKLTHSKNLAEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8FFD3</accession>
    <name>A0ABD8FFD3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 11">Adenylosuccinate synthetase</fullName>
            <shortName evidence="9">AMPSase</shortName>
            <shortName evidence="9">AdSS</shortName>
            <ecNumber evidence="9 11">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Adss</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9 11">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9 11">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 11">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="9 11"/>
    <dbReference type="RefSeq" id="XP_001358629.2">
        <property type="nucleotide sequence ID" value="XM_001358592.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8FFD3"/>
    <dbReference type="SMR" id="A0ABD8FFD3"/>
    <dbReference type="GeneID" id="4801557"/>
    <dbReference type="KEGG" id="dpo:4801557"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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            <name>IMP</name>
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            <name>Mg(2+)</name>
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            <note>ligand shared between dimeric partners</note>
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        <source>
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    <sequence length="448" mass="49281" checksum="B79B658E8B4D2A6C" modified="2025-06-18" version="1">MSTTNAINGNLYEQLHQGRTNMYKSKVDVVLGAQWGDEGKGKVVDMLASEVDIVCRCQGGNNAGHTVVANGTEFDFHLLPSGVVNEKCISVIGNGVVIHLPSLFDEVLKNEAKGLQHLEHRLIISDRAHLVFDFHQHVDGMQEAEKGGKSLGTTKKGIGPAYSSKATRNGIRVGELLGDFNAFSDKFKLIVATHLRLFPSINVDVEAELTRYRDYAEKVRPYVKDTICFLHTALRNGKTILVEGANAAMLDIDFGTYPYVTSSNCSIGGVLTGLGLPPQTIGEVIGVVKAYTTRVGDGPFPSEQLNEIGDLLQTRGFEVGVTTKRKRRCGWLDIPLLRYTSLVNGYTCICLTKLDILDTLPEIKVAVSYKKANGDKLDHFPGTIAELGNIEVEYAVLPGWQTSTEHIRNFKELPENAQNYVRFLEKELSVPVRWVGVGKGRESIINVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-07-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>B2YE16</accession>
    <name>B2YE16_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 24">Cytochrome c oxidase subunit 1</fullName>
            <ecNumber evidence="7 24">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">COI</name>
        <name evidence="29" type="synonym">COX1</name>
        <name evidence="29" type="ORF">HCS66_mgp02</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="27 29"/>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2008" name="Biol. Lett." volume="4" first="195" last="199">
            <title>Out of Hawaii: the origin and biogeography of the genus Scaptomyza (Diptera: Drosophilidae).</title>
            <authorList>
                <person name="O'Grady P.M."/>
                <person name="DeSalle R."/>
            </authorList>
            <dbReference type="PubMed" id="18296276"/>
            <dbReference type="DOI" id="10.1098/rsbl.2007.0575"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2008-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="O'Grady P.M."/>
                <person name="DeSalle R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="28 29" key="3">
        <citation type="submission" date="2019-12" db="EMBL/GenBank/DDBJ databases">
            <title>Evolutionary conservation of topologically associating domains and their influences on genome synteny and structural variations in Drosophila.</title>
            <authorList>
                <person name="Liao Y."/>
                <person name="Chakraborty M."/>
                <person name="Zhang X."/>
                <person name="Emerson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28 29">MV2-25</strain>
            <tissue evidence="29">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="28 29" key="4">
        <citation type="submission" date="2020-03" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="NCBI Genome Project"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28 29">MV2-25</strain>
            <tissue evidence="29">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="29" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="29">MV2-25</strain>
            <tissue evidence="29">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="24">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:11437"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 24">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 24">Mitochondrion inner membrane</location>
            <topology evidence="3 24">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="7 24"/>
    <dbReference type="EMBL" id="EU493633">
        <property type="protein sequence ID" value="ACD54949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_009742607.1">
        <property type="nucleotide sequence ID" value="NC_046603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B2YE16"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Mitochondrion MT"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01663">
        <property type="entry name" value="Cyt_c_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.210.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033944">
        <property type="entry name" value="Cyt_c_oxase_su1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20 24" id="KW-0186">Copper</keyword>
    <keyword evidence="17 24" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 24" id="KW-0349">Heme</keyword>
    <keyword evidence="19 24" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="22 24 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21 24 27" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14 24" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 24" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="16" id="KW-1278">Translocase</keyword>
    <keyword evidence="12 24 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 24" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="26">
        <location>
            <begin position="1"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="27">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000369"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="ACD54949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="YP_009742607.1"/>
        </source>
    </evidence>
    <sequence length="511" mass="56241" checksum="8972CF6035971EB3" modified="2008-07-01" version="1" fragment="single">SRQWLFSTNHKDIGTLYFIFGAWAGMVGTSLSILIRAELGHPGALIGDDQIYNVIVTAHAFIMIFFMVMPIMIGGFGNWLVPLMLGAPDMAFPRMNNMSFWLLPPALSLLLVSSMVENGAGTGWTVYPPLSSGIAHGGASVDLAIFSLHLAGISSILGAVNFITTVINMRSSGITLDRMPLFVWSVVITALLLLLSLPVLAGAITMLLTDRNLNTSFFDPAGGGDPILYQHLFWFFGHPEVYILILPGFGMISHIISQESGKKETFGSLGMIYAMLAIGLLGFIVWAHHMFTVGMDVDTRAYFTSATMIIAVPTGIKIFSWLATLHGAQLSYSPAILWALGFVFLFTVGGLTGVVLANSSVDIILHDTYYVVAHFHYVLSMGAVFAIMAGFIHWFPLFTGLTMNNKLLKSQFVIMFIGVNLTFFPQHFLGLAGMPRRYSDYPDAYTTWNVVSTIGSSISLLGILFFFYIIWESLVSQRQVIYPIQLCSSIEWYQNTPPAEHSYSELPLLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPE8</accession>
    <accession>A0A6I8V0I9</accession>
    <name>B5DPE8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trafficking protein particle complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">gry</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endoplasmic reticulum to Golgi apparatus trafficking at a very early stage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAPPC11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134900.1">
        <property type="nucleotide sequence ID" value="XM_002134864.3"/>
    </dbReference>
    <dbReference type="SMR" id="B5DPE8"/>
    <dbReference type="FunCoup" id="B5DPE8">
        <property type="interactions" value="2317"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DPE8"/>
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    <dbReference type="KEGG" id="dpo:6900511"/>
    <dbReference type="eggNOG" id="KOG4386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003649_1_0_1"/>
    <dbReference type="InParanoid" id="B5DPE8"/>
    <dbReference type="OMA" id="IIHVEKY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244947">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DPE8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021773">
        <property type="entry name" value="TPC11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025876">
        <property type="entry name" value="TRAPPC11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374">
        <property type="entry name" value="FOIE GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11817">
        <property type="entry name" value="Foie-gras_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12742">
        <property type="entry name" value="Gryzun-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 11" evidence="9">
        <location>
            <begin position="265"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking protein particle complex subunit 11 C-terminal" evidence="10">
        <location>
            <begin position="1235"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="878"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1035"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1058"/>
            <end position="1067"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001995"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007051"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002134900.1"/>
        </source>
    </evidence>
    <sequence length="1328" mass="149467" checksum="F2CDA5C028EF3919" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPP5</accession>
    <accession>A0A6I8V0D8</accession>
    <name>B5DPP5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Pcyt2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="10"/>
    <dbReference type="RefSeq" id="XP_002134940.1">
        <property type="nucleotide sequence ID" value="XM_002134904.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPP5"/>
    <dbReference type="SMR" id="B5DPP5"/>
    <dbReference type="STRING" id="46245.B5DPP5"/>
    <dbReference type="GeneID" id="6900562"/>
    <dbReference type="KEGG" id="dpo:6900562"/>
    <dbReference type="eggNOG" id="KOG2804">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034585_4_1_1"/>
    <dbReference type="InParanoid" id="B5DPP5"/>
    <dbReference type="OMA" id="FEDFSIC"/>
    <dbReference type="UniPathway" id="UPA00753">
        <property type="reaction ID" value="UER00739"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244920">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="113"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="340"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002134940.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="47141" checksum="7E2EDFAFE537226A" modified="2008-10-14" version="1">MDKLIEEEASPSSSEHGTPSPASRSGRSTPSYEFQMDKLLEDVDSTDSELPEWESFPTLPLYDMNFDKFPFCKPCPFANDVQALDELNGCDYTQRISYEMAHSGQSKRRVRVYADGIYDLFHQGHARQLMQAKNIFPNVYLIVGVCNDELTLKMKGRTVMNGFERYEAVRHCRYVDEVVPNAPWTLTDDFLTEHKIDFVAHDDIPYGAGGVNDIYAPLKARGMFVATERTEGVSTSDVVARIVKDYDVYVRRNLARGYSAKELNVSFLSEKKFRLQNKMDELKDRGKRVKVDIIAKWEEKSREFIDAFLQAFGRDRLHSFWNESKGKIMSAWSSTASINCDDPSAMHSPDGSDSNDEYEESHSVARSDSGIDSRRHGSGAADLGNLNRSSSFPVVDLGSTRAPPQEFDLVKPKIVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPQ3</accession>
    <accession>A0A6I8V0L1</accession>
    <name>B5DPQ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="7">6.2.1.4</ecNumber>
            <ecNumber evidence="7">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="7">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Scsalpha1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.2.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with an ATP-specific beta subunit, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with a GTP-specific beta subunit forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.4" evidence="7"/>
    <dbReference type="EC" id="6.2.1.5" evidence="7"/>
    <dbReference type="RefSeq" id="XP_002134933.1">
        <property type="nucleotide sequence ID" value="XM_002134897.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPQ3"/>
    <dbReference type="SMR" id="B5DPQ3"/>
    <dbReference type="FunCoup" id="B5DPQ3">
        <property type="interactions" value="1955"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DPQ3"/>
    <dbReference type="GeneID" id="6900549"/>
    <dbReference type="KEGG" id="dpo:6900549"/>
    <dbReference type="eggNOG" id="KOG1255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_1_0_1"/>
    <dbReference type="InParanoid" id="B5DPQ3"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245150">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DPQ3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000005">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="10">
        <location>
            <begin position="33"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="7 8">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="46"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002134933.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="34318" checksum="5F98D784D9070C54" modified="2008-10-14" version="1">MAASMRALLKVRDGFVAGVRCNSQYNKTRGNLKLTGDSKVICQGFTGKQGTFHSQQALEYGTKLVGGISPKKGGTQHLGLPVFASVAEAKKATDPHATVIYVPPPGAAAAIIEALDAEIPLIVCITEGVPQHDMVKVKHALISQSKSRLVGPNCPGIIAPEQCKIGIMPGHIHKRGKIGVVSRSGTLTYEAVHQTTEVGLGQTLCVGIGGDPFNGTDFIDCLEVFLKDPDTKGIILIGEIGGVAEEKAADYLTEFNTGIKAKPVVSFIAGVSAPPGRRMGHAGAIISGGKGGANDKIAALEKAGVIVTRSPAKMGHELFKEMKRLELV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1987-08-13" modified="2026-01-28" version="173" xmlns="https://uniprot.org/uniprot">
    <accession>P04809</accession>
    <accession>O16059</accession>
    <accession>O16060</accession>
    <accession>O16061</accession>
    <accession>O16062</accession>
    <accession>O16063</accession>
    <accession>O16064</accession>
    <accession>O16065</accession>
    <accession>O16066</accession>
    <accession>O16067</accession>
    <accession>O18670</accession>
    <accession>Q2M0F2</accession>
    <name>HSP83_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Heat shock protein 83</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>HSP 82</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">Hsp83</name>
        <name type="synonym">Hsp82</name>
        <name type="ORF">GA11622</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1986" name="J. Mol. Biol." volume="188" first="499" last="515">
            <title>Interspecific nucleotide sequence comparisons used to identify regulatory and structural features of the Drosophila hsp82 gene.</title>
            <authorList>
                <person name="Blackman R.K."/>
                <person name="Meselson M."/>
            </authorList>
            <dbReference type="PubMed" id="2426456"/>
            <dbReference type="DOI" id="10.1016/S0022-2836(86)80001-8"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-375</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1997" name="Genetics" volume="147" first="1091" last="1106">
            <title>Gene flow and natural selection in the origin of Drosophila pseudoobscura and close relatives.</title>
            <authorList>
                <person name="Wang R.L."/>
                <person name="Wakeley J."/>
                <person name="Hey J."/>
            </authorList>
            <dbReference type="PubMed" id="9383055"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-269</scope>
        <scope>VARIANTS</scope>
        <source>
            <strain>pseudo1</strain>
            <strain>pseudo10</strain>
            <strain>pseudo11</strain>
            <strain>pseudo2</strain>
            <strain>pseudo3</strain>
            <strain>pseudo4</strain>
            <strain>pseudo5</strain>
            <strain>pseudo6</strain>
            <strain>pseudo7</strain>
            <strain>pseudo8</strain>
            <strain>pseudo9</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Required for piRNA biogenesis by facilitating loading of piRNAs into PIWI proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Interacts with shu.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL30982" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CH379069">
        <property type="protein sequence ID" value="EAL30982.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X03812">
        <property type="protein sequence ID" value="CAA27439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006529">
        <property type="protein sequence ID" value="AAC07914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006530">
        <property type="protein sequence ID" value="AAC07915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006531">
        <property type="protein sequence ID" value="AAC07916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006532">
        <property type="protein sequence ID" value="AAC07917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006533">
        <property type="protein sequence ID" value="AAC07918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006534">
        <property type="protein sequence ID" value="AAC07919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006535">
        <property type="protein sequence ID" value="AAC07920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006536">
        <property type="protein sequence ID" value="AAC07921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006537">
        <property type="protein sequence ID" value="AAC07922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006538">
        <property type="protein sequence ID" value="AAC07923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF006539">
        <property type="protein sequence ID" value="AAC07924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C24827">
        <property type="entry name" value="C24827"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001353471.1">
        <property type="nucleotide sequence ID" value="XM_001353435.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P04809"/>
    <dbReference type="SMR" id="P04809"/>
    <dbReference type="FunCoup" id="P04809">
        <property type="interactions" value="1408"/>
    </dbReference>
    <dbReference type="STRING" id="46245.P04809"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0276082">
        <property type="protein sequence ID" value="FBpp0274520"/>
        <property type="gene ID" value="FBgn0012701"/>
    </dbReference>
    <dbReference type="GeneID" id="4813013"/>
    <dbReference type="KEGG" id="dpo:4813013"/>
    <dbReference type="CTD" id="38389"/>
    <dbReference type="eggNOG" id="KOG0019">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006684_1_3_1"/>
    <dbReference type="InParanoid" id="P04809"/>
    <dbReference type="OMA" id="MRRMKEM"/>
    <dbReference type="PhylomeDB" id="P04809"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0012701">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000001">
        <property type="entry name" value="Heat shock protein HSP 90-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000062934" description="Heat shock protein 83">
        <location>
            <begin position="1"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="687"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TPR repeat-binding">
        <location>
            <begin position="713"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="sequence variant" description="In strain: pseudo8 and pseudo9.">
        <original>G</original>
        <variation>S</variation>
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="717" mass="81771" checksum="BF46AF2207322273" modified="2006-05-30" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DP1</accession>
    <accession>A0A6I8UBJ3</accession>
    <name>Q29DP1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tryptophan 5-hydroxylase 2</fullName>
            <ecNumber evidence="4">1.14.16.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Tryptophan 5-monooxygenase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">Trhn</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tryptophan + O2 = 5-hydroxy-L-tryptophan + (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin</text>
            <dbReference type="Rhea" id="RHEA:16709"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58266"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="EC" id="1.14.16.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with DNAJC12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001352872.2">
        <property type="nucleotide sequence ID" value="XM_001352836.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DP1"/>
    <dbReference type="SMR" id="Q29DP1"/>
    <dbReference type="FunCoup" id="Q29DP1">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DP1"/>
    <dbReference type="GeneID" id="4812462"/>
    <dbReference type="KEGG" id="dpo:4812462"/>
    <dbReference type="eggNOG" id="KOG3820">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023198_0_2_1"/>
    <dbReference type="InParanoid" id="Q29DP1"/>
    <dbReference type="OMA" id="DMPWFPR"/>
    <dbReference type="UniPathway" id="UPA00846">
        <property type="reaction ID" value="UER00799"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081546">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004510">
        <property type="term" value="F:tryptophan 5-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009072">
        <property type="term" value="P:aromatic amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042416">
        <property type="term" value="P:dopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042427">
        <property type="term" value="P:serotonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04929">
        <property type="entry name" value="ACT_TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03346">
        <property type="entry name" value="eu_TrpOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.800.10:FF:000004">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005963">
        <property type="entry name" value="Trp_5_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041904">
        <property type="entry name" value="TrpOH_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01270">
        <property type="entry name" value="Trp_5_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF16">
        <property type="entry name" value="TRYPTOPHAN 5-HYDROXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0408">Iron</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0724">Serotonin biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="19">
        <location>
            <begin position="98"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="18">
        <location>
            <begin position="181"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="551"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="556"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001352872.2"/>
        </source>
    </evidence>
    <sequence length="572" mass="63498" checksum="F313211EF31B50E2" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EY6</accession>
    <accession>A0A6I8UAH5</accession>
    <name>Q29EY6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fork head domain-containing protein FD2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FoxL1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352427.1">
        <property type="nucleotide sequence ID" value="XM_001352391.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EY6"/>
    <dbReference type="SMR" id="Q29EY6"/>
    <dbReference type="FunCoup" id="Q29EY6">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EY6"/>
    <dbReference type="GeneID" id="4811884"/>
    <dbReference type="KEGG" id="dpo:4811884"/>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_797590_0_0_1"/>
    <dbReference type="InParanoid" id="Q29EY6"/>
    <dbReference type="OMA" id="SLFNNEP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070971">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EY6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF388">
        <property type="entry name" value="FORK HEAD DOMAIN-CONTAINING PROTEIN L1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="DNA-binding region" description="Fork-head" evidence="7">
        <location>
            <begin position="86"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="182"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001352427.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="41512" checksum="2DC200C892BF377B" modified="2006-04-04" version="1">MLPSCYSNGSVLPDNEDLVNSLLANPEYLRTSANPLGTSAVGMDSLMCSSFPSAFYYQGIDNFLALHNNIWTLPISFLHNTHRPEKPPFSYIALIAMAISSAPNQRLTLSGIYKFIMDKFPYYRDNKQGWQNSIRHNLSLNDCFVKVPRDKNTIDDNDSAGKGSYWMLDASASDMFEQGNYRRRRTRRQRHCTNASRYERDAGKDATDGSSTAEIRSPSDSLNDFDIFCNERPNYSDRITDLHRQYLSVSLGFNSLFNGEASGVRQLPGASTVVRESNADVDAEVDGDASTDSPCSSSSKTAQNIPDSASALHDELHSPSAFTPTHNHRLPGLSDAFKGLQHLMPSNTAPAPGTGTTAANSRSKTTLFTIDNIIGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FB3</accession>
    <accession>A0A6I8UEP3</accession>
    <name>Q29FB3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl-coenzyme A thioesterase 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4814099</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354290.2">
        <property type="nucleotide sequence ID" value="XM_001354254.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FB3"/>
    <dbReference type="SMR" id="Q29FB3"/>
    <dbReference type="FunCoup" id="Q29FB3">
        <property type="interactions" value="993"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FB3"/>
    <dbReference type="GeneID" id="4814099"/>
    <dbReference type="KEGG" id="dpo:4814099"/>
    <dbReference type="eggNOG" id="KOG3328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_12_2_1"/>
    <dbReference type="InParanoid" id="Q29FB3"/>
    <dbReference type="OMA" id="IIDWAGG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074287">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FB3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000033">
        <property type="entry name" value="acyl-coenzyme A thioesterase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="58"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354290.2"/>
        </source>
    </evidence>
    <sequence length="144" mass="15845" checksum="91DE297E79CFFCB8" modified="2008-10-14" version="2">MGTRKKGLDFAKHISEIIAKSTGFENHMKKVKIIGGGDGTCQAELKVEADHVNPYNGLHGGYIVTLVDMITTYALMSKPCHPGVSVDINVNYLNAARLGDEVRIEANISKIGKYLAFIECTLRHKKDDSVIAKGTHMKYVNIKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FS3</accession>
    <name>RTEL1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Regulator of telomere elongation helicase 1 homolog</fullName>
            <ecNumber evidence="1">5.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA17940</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">A probable ATP-dependent DNA helicase implicated in DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CH379065">
        <property type="protein sequence ID" value="EAL31506.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FS3"/>
    <dbReference type="SMR" id="Q29FS3"/>
    <dbReference type="FunCoup" id="Q29FS3">
        <property type="interactions" value="1953"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FS3"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0273691">
        <property type="protein sequence ID" value="FBpp0272129"/>
        <property type="gene ID" value="FBgn0077949"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814338"/>
    <dbReference type="eggNOG" id="KOG1132">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006515_4_0_1"/>
    <dbReference type="InParanoid" id="Q29FS3"/>
    <dbReference type="OMA" id="NCATIVA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077949">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904430">
        <property type="term" value="P:negative regulation of t-circle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010569">
        <property type="term" value="P:regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090657">
        <property type="term" value="P:telomeric loop disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17970">
        <property type="entry name" value="DEAHc_FancJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13932">
        <property type="entry name" value="HN_RTEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000431">
        <property type="entry name" value="Regulator of telomere elongation helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.20:FF:000011">
        <property type="entry name" value="Regulator of telomere elongation helicase 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03065">
        <property type="entry name" value="RTEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030845">
        <property type="entry name" value="RTEL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057498">
        <property type="entry name" value="Rtel1_ARCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049909">
        <property type="entry name" value="Rtel1_HHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23109">
        <property type="entry name" value="ARCH_RTEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23116">
        <property type="entry name" value="HHD_RTEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000370626" description="Regulator of telomere elongation helicase 1 homolog">
        <location>
            <begin position="1"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box">
        <location>
            <begin position="252"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03065"/>
        </source>
    </evidence>
    <sequence length="1009" mass="112164" checksum="F4A06894F01B09E3" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GE4</accession>
    <accession>A0A6I8UGI0</accession>
    <name>Q29GE4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein IWS1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4814897</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IWS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355109.2">
        <property type="nucleotide sequence ID" value="XM_001355073.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GE4"/>
    <dbReference type="SMR" id="Q29GE4"/>
    <dbReference type="STRING" id="46245.Q29GE4"/>
    <dbReference type="GeneID" id="4814897"/>
    <dbReference type="KEGG" id="dpo:4814897"/>
    <dbReference type="eggNOG" id="KOG1793">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018315_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GE4"/>
    <dbReference type="OMA" id="QNRDRAG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082100">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29GE4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.10:FF:000001">
        <property type="entry name" value="IWS1, SUPT6H interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="536"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="128"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="172"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="555"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355109.2"/>
        </source>
    </evidence>
    <sequence length="656" mass="72614" checksum="8307024ED634DED5" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GI5</accession>
    <accession>A0A6I8UGC9</accession>
    <name>Q29GI5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Negative elongation factor D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TH1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NELF-D family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355068.1">
        <property type="nucleotide sequence ID" value="XM_001355032.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GI5"/>
    <dbReference type="SMR" id="Q29GI5"/>
    <dbReference type="FunCoup" id="Q29GI5">
        <property type="interactions" value="1678"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GI5"/>
    <dbReference type="GeneID" id="4814833"/>
    <dbReference type="KEGG" id="dpo:4814833"/>
    <dbReference type="eggNOG" id="ENOG502QPUE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028060_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GI5"/>
    <dbReference type="OMA" id="CHSEHTY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082145">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006942">
        <property type="entry name" value="TH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12144:SF0">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12144">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04858">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001355068.1"/>
        </source>
    </evidence>
    <sequence length="578" mass="65599" checksum="71CF3D7BFC705CEF" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HN9</accession>
    <accession>A0A6I8UFH0</accession>
    <name>Q29HN9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioredoxin domain-containing protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4814802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354664.2">
        <property type="nucleotide sequence ID" value="XM_001354628.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HN9"/>
    <dbReference type="SMR" id="Q29HN9"/>
    <dbReference type="FunCoup" id="Q29HN9">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HN9"/>
    <dbReference type="GeneID" id="4814802"/>
    <dbReference type="KEGG" id="dpo:4814802"/>
    <dbReference type="eggNOG" id="KOG3425">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120161_0_0_1"/>
    <dbReference type="InParanoid" id="Q29HN9"/>
    <dbReference type="OMA" id="LLHWKGV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077798">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047134">
        <property type="term" value="F:protein-disulfide reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045108">
        <property type="entry name" value="TXNDC17-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010357">
        <property type="entry name" value="TXNDC17_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12452">
        <property type="entry name" value="42-9-9 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12452:SF6">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06110">
        <property type="entry name" value="TXD17-like_Trx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354664.2"/>
        </source>
    </evidence>
    <sequence length="141" mass="16078" checksum="BEEE6A9041DD9AD2" modified="2008-10-14" version="2">MTEYVPVRGYQELQNFLKAYENKQGPIYLYFYGEKDSKGKSWCPDCVDAEETIMTAFRNHARQAIVLVIDVGNRDAWMDSSDKNGFRKPPLSLSSIPSLLHWKGVERLEGDQLLKSSLLELFFEETSLKSGPPSSARNINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q29I28</accession>
    <accession>A0A6I8UF46</accession>
    <name>Q29I28_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Synaptobrevin homolog YKT6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Ykt6</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Cytoplasmic vesicle membrane</location>
            <topology evidence="18">Lipid-anchor</topology>
            <orientation evidence="18">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354525.1">
        <property type="nucleotide sequence ID" value="XM_001354489.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29I28"/>
    <dbReference type="SMR" id="Q29I28"/>
    <dbReference type="FunCoup" id="Q29I28">
        <property type="interactions" value="2583"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29I28"/>
    <dbReference type="GeneID" id="4814944"/>
    <dbReference type="KEGG" id="dpo:4814944"/>
    <dbReference type="eggNOG" id="KOG0861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074848_2_1_1"/>
    <dbReference type="InParanoid" id="Q29I28"/>
    <dbReference type="OMA" id="HYIGIIR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073567">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29I28">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000020">
        <property type="entry name" value="synaptobrevin homolog YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000013">
        <property type="entry name" value="Synaptobrevin homolog YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00025701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001354525.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="22417" checksum="E389C23B86B9FB03" modified="2006-04-04" version="1">MVKLFALSVFFKGASEARILKTASDLQSFSFFQRSTVNEFMTFASKTIVERTQPALRQSVKQDAYMCHVYVRADNLAGVLIADHEYPHRVAHTLITKILDDFTAKVSADQWPNGTEATIAFDLLPAFLSKYQNPVEADPLTKMQNDLDETTIILKNTIEAVLERGEKLDDMVNKSEKLSIQSKAFYKTAKKTNSCCSFT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IC2</accession>
    <name>RIC8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Chaperone Ric-8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Synembryn</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ric8a</name>
        <name type="synonym">Ric8</name>
        <name type="ORF">GA13961</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Chaperone that specifically binds and folds some, but not all, nascent G alpha proteins prior to G protein heterotrimer formation, promoting their stability and activity (By similarity). Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP (By similarity). Plays a key role in asymmetric spindle positioning, a step for asymmetric cell division that generates cell diversity during development by activating G(i) alpha protein independently of G-protein coupled receptors. Required during gastrulation and sensory organ precursor (SOP) formation. Plays a role in positively regulating synapse number and neurotransmitter release.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with GDP-bound G(i)-alpha protein. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma. Interacts with Frq2 in a Ca(2+)-independent manner but does not interact with Frq1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Presynapse</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Detected in both type Is boutons and Ib boutons in close proximity to synaptic active zones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synembryn family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379063">
        <property type="protein sequence ID" value="EAL32731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IC2"/>
    <dbReference type="SMR" id="Q29IC2"/>
    <dbReference type="FunCoup" id="Q29IC2">
        <property type="interactions" value="1899"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IC2"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0274228">
        <property type="protein sequence ID" value="FBpp0272666"/>
        <property type="gene ID" value="FBgn0073993"/>
    </dbReference>
    <dbReference type="GeneID" id="4816097"/>
    <dbReference type="KEGG" id="dpo:4816097"/>
    <dbReference type="CTD" id="60626"/>
    <dbReference type="eggNOG" id="KOG4464">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018602_1_0_1"/>
    <dbReference type="InParanoid" id="Q29IC2"/>
    <dbReference type="OMA" id="NADPIFT"/>
    <dbReference type="PhylomeDB" id="Q29IC2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073993">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008356">
        <property type="term" value="P:asymmetric cell division"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010004">
        <property type="term" value="P:gastrulation involving germ band extension"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055057">
        <property type="term" value="P:neuroblast division"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008376">
        <property type="entry name" value="Chaperone_Ric-8_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019318">
        <property type="entry name" value="Gua_nucleotide_exch_fac_Ric8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425:SF5">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10165">
        <property type="entry name" value="Ric8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01802">
        <property type="entry name" value="SYNEMBRYN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0966">Cell projection</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0770">Synapse</keyword>
    <feature type="chain" id="PRO_0000235906" description="Chaperone Ric-8">
        <location>
            <begin position="1"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9GSX9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9W358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="566" mass="64450" checksum="AAED9E18F0E9008C" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IN6</accession>
    <accession>A0A6I8UHH7</accession>
    <name>Q29IN6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Kmn1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Kmn1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355558.2">
        <property type="nucleotide sequence ID" value="XM_001355522.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IN6"/>
    <dbReference type="FunCoup" id="Q29IN6">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IN6"/>
    <dbReference type="GeneID" id="4815566"/>
    <dbReference type="KEGG" id="dpo:4815566"/>
    <dbReference type="eggNOG" id="ENOG502TBIG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1476640_0_0_1"/>
    <dbReference type="InParanoid" id="Q29IN6"/>
    <dbReference type="OMA" id="GHLEFRK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073860">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IN6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355558.2"/>
        </source>
    </evidence>
    <sequence length="187" mass="21960" checksum="2486A92A746A48C3" modified="2008-10-14" version="2">MNPTTDDVPNELGHDISDIDLHDVPAQLENTLKPKFHETKSLFNINIDEIFETTTGDEEVQEYKDDIKARKQKWIDFMVDATKKGEEALKTYEGTLDNREDLNPIPEVKPNAEQQAYLDQGPHFQNFIRGHLEFRKNYEVFKQKHEEMRELQARIMERCRLRLNIVVANFFNQNLGTETTKKPLKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q29J46</accession>
    <accession>A0A6I8UH31</accession>
    <name>Q29J46_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4815577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355398.2">
        <property type="nucleotide sequence ID" value="XM_001355362.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29J46"/>
    <dbReference type="FunCoup" id="Q29J46">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="4815577"/>
    <dbReference type="KEGG" id="dpo:4815577"/>
    <dbReference type="eggNOG" id="ENOG502T6YA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146238_0_0_1"/>
    <dbReference type="InParanoid" id="Q29J46"/>
    <dbReference type="OMA" id="RNYYEAS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073607">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29J46">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355398.2"/>
        </source>
    </evidence>
    <sequence length="127" mass="13500" checksum="EFC509AF70604588" modified="2008-10-14" version="2">MKQIPGLLGLGLVLAGLLLAAGTSNALPMTNDLELVAALDAMEPAAAAAQPSHISRIAARGEATAHEDRPELFNTIVQELKAAGVYTAERQDLHTLSYKELTRLLALWHLSSGRNYYEASPAPEIVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JI9</accession>
    <name>MCM6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>DNA replication licensing factor Mcm6</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Mcm6</name>
        <name type="ORF">GA17904</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity Required for DNA replication and cell proliferation. Required for mitotic cycles, endocycles, and the special S phase associated with the amplification of chorion genes; has a role in origin unwinding or fork elongation at chorion loci.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5. The heterodimers of mcm4/mcm6 and mcm3/mcm5 interact with mcm2 and mcm7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <text evidence="1">Associated with chromatin during cell cycles.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MCM family.</text>
    </comment>
    <comment type="sequence caution" evidence="7">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL32312" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CH379063">
        <property type="protein sequence ID" value="EAL32312.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001355255.1">
        <property type="nucleotide sequence ID" value="XM_001355219.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JI9"/>
    <dbReference type="SMR" id="Q29JI9"/>
    <dbReference type="FunCoup" id="Q29JI9">
        <property type="interactions" value="1529"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JI9"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0274750">
        <property type="protein sequence ID" value="FBpp0273188"/>
        <property type="gene ID" value="FBgn0077913"/>
    </dbReference>
    <dbReference type="GeneID" id="4816084"/>
    <dbReference type="KEGG" id="dpo:4816084"/>
    <dbReference type="CTD" id="4175"/>
    <dbReference type="eggNOG" id="KOG0480">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_3_2_1"/>
    <dbReference type="InParanoid" id="Q29JI9"/>
    <dbReference type="OMA" id="RHQQTDK"/>
    <dbReference type="PhylomeDB" id="Q29JI9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077913">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.870:FF:000001">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000091">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000004">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000115">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF43">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0498">Mitosis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000233316" description="DNA replication licensing factor Mcm6">
        <location>
            <begin position="1"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="5">
        <location>
            <begin position="321"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="4">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="672"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Walker A" evidence="5">
        <location>
            <begin position="388"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Walker B" evidence="5">
        <location>
            <begin position="452"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="678"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared with MCM4</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared with MCM4</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared with MCM4</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared with MCM4</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared with MCM4</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared with MCM4</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="611"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <note>ligand shared with MCM2</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="614"/>
        </location>
        <ligand>
            <name>ADP</name>
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            <note>ligand shared with MCM2</note>
        </ligand>
    </feature>
    <feature type="site" description="Arginine finger" evidence="5">
        <location>
            <position position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P97311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q14566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="EMBL" id="EAL32312.1"/>
        </source>
    </evidence>
    <sequence length="815" mass="91994" checksum="932D6C1BDA572D33" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KW9</accession>
    <accession>A0A6I8UIF8</accession>
    <name>Q29KW9_DROPS</name>
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        <recommendedName>
            <fullName evidence="6 16">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="6 16">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Pgk</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="6 16"/>
    <dbReference type="RefSeq" id="XP_001355994.1">
        <property type="nucleotide sequence ID" value="XM_001355958.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KW9"/>
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    </dbReference>
    <dbReference type="STRING" id="46245.Q29KW9"/>
    <dbReference type="GeneID" id="4816379"/>
    <dbReference type="KEGG" id="dpo:4816379"/>
    <dbReference type="eggNOG" id="KOG1367">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_0_1"/>
    <dbReference type="InParanoid" id="Q29KW9"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076155">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KW9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:phosphoglycerate kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:glycolytic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate kinase 1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate_kinase"/>
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        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
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    </dbReference>
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        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 16 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="371"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001355994.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="43992" checksum="A26810FDAC6F263E" modified="2006-04-04" version="1">MAFNKLSIENLDLEGKRVLMRVDFNVPIKDGKITSNQRIVAALDSVKLALSKKAKSVVLMSHLGRPDGNKNIKYTLAPVADELKKLLGQDVKFLSDCVGSEVEAACKDPAPGSVILLENVRFYVEEEGKGLDASGGKVKADPAKVKEFRSSLAKLGDVYVNDAFGTAHRAHSSMMGDGFEKRAAGLLLNKELKYFSQALDNPPNPFLAILGGAKVADKIQLIENLLDKVNEMIIGGGMAFTFLKVLNNMKIGGSLFDEEGSKIVQNLVEKAKKNNVKLHLPVDFVCGDKFAENAVVSEATVESGIPDGHMGLDVGPKTRELFAAPVARAKLIVWNGPPGVFEFPNFANGTKGIMDGVVAATKNGTISIIGGGDTASCCAKWNTEALVSHVSTGGGASLELLEGKTLPGVAALSNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MT7</accession>
    <name>NUBP1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytosolic Fe-S cluster assembly factor Nubp1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">Nubp1</name>
        <name type="ORF">GA14715</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The Nubp1-Nubp2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of Nubp1 and two labile, bridging clusters between subunits of the Nubp1-Nubp2 heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of 2 Nubp1 and 2 Nubp2 chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CH379060">
        <property type="protein sequence ID" value="EAL33606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MT7"/>
    <dbReference type="SMR" id="Q29MT7"/>
    <dbReference type="FunCoup" id="Q29MT7">
        <property type="interactions" value="837"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MT7"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0281653">
        <property type="protein sequence ID" value="FBpp0280091"/>
        <property type="gene ID" value="FBgn0074742"/>
    </dbReference>
    <dbReference type="GeneID" id="4816827"/>
    <dbReference type="KEGG" id="dpo:4816827"/>
    <dbReference type="CTD" id="4682"/>
    <dbReference type="eggNOG" id="KOG3022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_1"/>
    <dbReference type="InParanoid" id="Q29MT7"/>
    <dbReference type="OMA" id="VSGCPMR"/>
    <dbReference type="PhylomeDB" id="Q29MT7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074742">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MT7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001759">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03038">
        <property type="entry name" value="NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028601">
        <property type="entry name" value="NUBP1/Nbp35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000382607" description="Cytosolic Fe-S cluster assembly factor Nubp1 homolog">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="63"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9VJI9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03038"/>
        </source>
    </evidence>
    <sequence length="311" mass="32839" checksum="F10020CC8E1F75AF" modified="2006-04-04" version="1">MQAPPPEHCPGVESEQAGQVSACAGCPNQSICSDPNKKREDPGKALVAAALKDVKHKLLILSGKGGVGKSTVTTLLTRYLARSYPDSNFGVLDIDICGPSQPRLMGALGENVHQSGSGWSPVGIDDNVCLMSIGFLLGSVDDAIIWRGPKKNGMIRQFLSEVDWGNLDLLLLDTPPGTSDEHLSVVSYLKDDSSPDSVHAIIVTTPQEVALLDVRKEINFCKKQQIPIVGVIENMSGFQCGHCGHSSEIFPAKTGGAAAMCAEMEVPLLGSLPLDPAIAKACDAGEDITSVKNPTTEALEGICSKIMSSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q29N51</accession>
    <accession>A0A6I8UJC5</accession>
    <name>Q29N51_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">SUMO-conjugating enzyme UBC9-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">lwr</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356428.1">
        <property type="nucleotide sequence ID" value="XM_001356392.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29N51"/>
    <dbReference type="SMR" id="Q29N51"/>
    <dbReference type="FunCoup" id="Q29N51">
        <property type="interactions" value="2591"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29N51"/>
    <dbReference type="GeneID" id="4817068"/>
    <dbReference type="KEGG" id="dpo:4817068"/>
    <dbReference type="eggNOG" id="KOG0424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_12_0_1"/>
    <dbReference type="InParanoid" id="Q29N51"/>
    <dbReference type="OMA" id="TWECGIP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075721">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29N51">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000013">
        <property type="entry name" value="SUMO-conjugating enzyme UBC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001356428.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="18164" checksum="6A20D7B8275CFEFB" modified="2006-04-04" version="1">MSGIAITRLGEERKAWRKDHPYGFVARPAKNPDGTLNLMIWECAIPGKKSTPWEGGLYKLRMIFKDDYPTSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNIKDPAQAEAYTIYCQNRLEYEKRVRAQARAMAATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q29N63</accession>
    <accession>A0A6I8VYC5</accession>
    <name>Q29N63_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4816870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236101.1">
        <property type="nucleotide sequence ID" value="XM_033380210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29N63"/>
    <dbReference type="FunCoup" id="Q29N63">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816870"/>
    <dbReference type="eggNOG" id="ENOG502T85B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_617177_0_0_1"/>
    <dbReference type="InParanoid" id="Q29N63"/>
    <dbReference type="OMA" id="WRTQPLE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073401">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033236101.1"/>
        </source>
    </evidence>
    <sequence length="446" mass="52047" checksum="A82FE66A2385B48A" modified="2008-10-14" version="2">MTTTTTADTAAPTPASGSASLLQLNDDVLALIIRHLNVYQQYELGKLHRRLENIVQMLWRTRVRNVALHDEMFKRSSPNSRQFLAFVLTLAPHMQRLSCQRLDVRRLRLLSSHTLSGVTSLEWLGDGHRRGRARFVDEDVRLLRRVFPSLRKLKLRGCQITGKYLCDLELLNELCLDDCQYLESQHFRDLFRQLKLRKFDIMEDCDEVNCCDLVDLQLCPTLEHIKIADYHLCMESDITQQLLQLPRLNKLSIYSKNFVFDVLSRIARPSYPTGRSRLIEGFRFSGILHDYERFFRELGNLRNLRRLELHGHGEEEGQLQCLGDQMLRHLSSALPELTELHLCGYQLESPLSLLGFVTHCRQLRVLDITRTRCHGDAFVWSCLAILNKQRWRTQPLELWIRKSDIKPEIMDSSRLMDNEKHIKIDCKQIPNSLDHTSGVLKFSFAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-03-23" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29P70</accession>
    <name>DRE2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anamorsin homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Fe-S cluster assembly protein DRE2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CIAPIN1</name>
        <name evidence="1" type="synonym">l(2)35Bg</name>
        <name type="ORF">GA18008</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the cytosolic Fe-S scaffold complex. Electrons are transferred from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Together with the diflavin oxidoreductase, also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anamorsin family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379058">
        <property type="protein sequence ID" value="EAL34423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001357354.1">
        <property type="nucleotide sequence ID" value="XM_001357318.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29P70"/>
    <dbReference type="FunCoup" id="Q29P70">
        <property type="interactions" value="2261"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29P70"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0280925">
        <property type="protein sequence ID" value="FBpp0279363"/>
        <property type="gene ID" value="FBgn0078017"/>
    </dbReference>
    <dbReference type="GeneID" id="4817989"/>
    <dbReference type="KEGG" id="dpo:4817989"/>
    <dbReference type="CTD" id="57019"/>
    <dbReference type="eggNOG" id="KOG4020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064393_1_0_1"/>
    <dbReference type="InParanoid" id="Q29P70"/>
    <dbReference type="OMA" id="GFINCRE"/>
    <dbReference type="PhylomeDB" id="Q29P70"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078017">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03115">
        <property type="entry name" value="Anamorsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049011">
        <property type="entry name" value="Anamorsin_N_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046408">
        <property type="entry name" value="CIAPIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273:SF14">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20922">
        <property type="entry name" value="Anamorsin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05093">
        <property type="entry name" value="CIAPIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000392320" description="Anamorsin homolog">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal SAM-like domain" evidence="1">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Linker" evidence="1">
        <location>
            <begin position="129"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fe-S binding site A" evidence="1">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fe-S binding site B" evidence="1">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 1" evidence="1">
        <location>
            <begin position="208"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 2" evidence="1">
        <location>
            <begin position="219"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03115"/>
        </source>
    </evidence>
    <sequence length="247" mass="26917" checksum="BA4D67CEE19012F7" modified="2006-04-04" version="1">MEQFKGLQKSLYIWTDSADLDKRVEQLKTATGGEVAVENVHRLSFSSYANSSFDLIVIECAQLTDNYVKLLHMLKPSGKLHLFSFIGPASSLLQEIKLSGFINCSEGTDTLTAEKPGYETGSSARLSFAKKNASALNVWKISGDDEELIDEEDLLDEEDKQKPDPAGLKVCSTTGKRKACKNCSCGLAEELESEKQTATASENAKSSCGNCYLGDAFRCSTCPYLGMPAFKPGEKVQLADNLLKSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ02</accession>
    <accession>A0A6I8UDX3</accession>
    <name>Q2LZ02_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein geko</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">geko</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353971.1">
        <property type="nucleotide sequence ID" value="XM_001353935.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ02"/>
    <dbReference type="GeneID" id="4813448"/>
    <dbReference type="KEGG" id="dpo:4813448"/>
    <dbReference type="eggNOG" id="ENOG502SAJ8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1350171_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LZ02"/>
    <dbReference type="OMA" id="GGMKSNH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072511">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZ02">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353971.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="23192" checksum="9E7F753DDE79EF75" modified="2006-02-21" version="1">MALMDTAWRCRLMIFGIVSLLLVGDLFGYGGGMKSDHGDNHSVGVRSGAVAGAGAARAGAPGAPTGPGPAAAAASKFQQSKSAEIDITEEHDFNEFSAAAALKPGMAVVTGASLKTKSQLTDTIKESCLPKMLCELASKPDYQLSEKERELLRLIRSPTMPWMMNMPPSKWYFAAHMGELMRHTGDNLSGPMGCANLWPNCPISSKKLMKLSYKVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NT97</accession>
    <accession>A0A6I8V8L9</accession>
    <name>A0A0R3NT97_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036082.1">
        <property type="nucleotide sequence ID" value="XM_015180596.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NT97"/>
    <dbReference type="GeneID" id="26533009"/>
    <dbReference type="KEGG" id="dpo:26533009"/>
    <dbReference type="InParanoid" id="A0A0R3NT97"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271996">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NT97">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015036082.1"/>
        </source>
    </evidence>
    <sequence length="50" mass="5575" checksum="4B86C0511E63FBBC" modified="2016-01-20" version="1">MPKITRTDSKQKLFKQSRLGKCLSSRKGSRTGNSANNKELKVLTLLQSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NWT4</accession>
    <accession>A0A6I8V8Z9</accession>
    <name>A0A0R3NWT4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Acetylcholine receptor subunit alpha-type acr-16 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">nAChRalpha6</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035733.1">
        <property type="nucleotide sequence ID" value="XM_015180247.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NWT4"/>
    <dbReference type="FunCoup" id="A0A0R3NWT4">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3NWT4"/>
    <dbReference type="GeneID" id="4817310"/>
    <dbReference type="InParanoid" id="A0A0R3NWT4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077986">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18997">
        <property type="entry name" value="LGIC_ECD_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000064">
        <property type="entry name" value="Neuronal acetylcholine receptor subunit alpha-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000058">
        <property type="entry name" value="Nicotinic acetylcholine receptor alpha6, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000059">
        <property type="entry name" value="Nicotinic acetylcholine receptor alpha6, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000016">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="17">
        <location>
            <begin position="30"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="18">
        <location>
            <begin position="301"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015035733.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="59156" checksum="096605E3A04473C5" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P6X4</accession>
    <accession>A0A6I8VLK2</accession>
    <name>A0A0R3P6X4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KappaPI-actitoxin-Avd3d-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015043845.1">
        <property type="nucleotide sequence ID" value="XM_015188359.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P6X4"/>
    <dbReference type="SMR" id="A0A0R3P6X4"/>
    <dbReference type="FunCoup" id="A0A0R3P6X4">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="GeneID" id="6900120"/>
    <dbReference type="KEGG" id="dpo:6900120"/>
    <dbReference type="InParanoid" id="A0A0R3P6X4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015043845.1"/>
        </source>
    </evidence>
    <sequence length="93" mass="10898" checksum="EC467FDD58780B82" modified="2016-01-20" version="1">MKFILVLACLALYVAYTSAQDNCIGRPVFQVCTGGRDEGHNRSRRCAVRFMREMWWYNARARDCQKMRYLGCGGNNNRYCSLASCRRKCRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAS2</accession>
    <name>A0A6I8UAS2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WASH complex subunit homolog 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Strump</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the strumpellin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352530.3">
        <property type="nucleotide sequence ID" value="XM_001352494.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAS2"/>
    <dbReference type="FunCoup" id="A0A6I8UAS2">
        <property type="interactions" value="1784"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811987"/>
    <dbReference type="InParanoid" id="A0A6I8UAS2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAS2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140285">
        <property type="term" value="P:endosome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051125">
        <property type="term" value="P:regulation of actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019393">
        <property type="entry name" value="WASH_strumpellin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15691">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15691:SF6">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10266">
        <property type="entry name" value="Strumpellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352530.3"/>
        </source>
    </evidence>
    <sequence length="1194" mass="137643" checksum="B7DABD4AAE017BA6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB14</accession>
    <name>A0A6I8UB14_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Platelet glycoprotein V isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4812621</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
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    </reference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Cys-rich_flank_reg_C"/>
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    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
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    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
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        <property type="entry name" value="LRR_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR"/>
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            <begin position="1"/>
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        <location>
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            <end position="616"/>
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        <location>
            <begin position="84"/>
            <end position="97"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00082"/>
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    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352661.3"/>
        </source>
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    <sequence length="616" mass="70227" checksum="D09E7FD8B58EDD77" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB34</accession>
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        <recommendedName>
            <fullName evidence="7">Structure-specific endonuclease subunit SLX4</fullName>
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        <name evidence="11" type="primary">mus312</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the SLX4 family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001352686.4"/>
        </source>
    </evidence>
    <sequence length="1163" mass="130618" checksum="0E2A5C409442C341" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB68</accession>
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            <fullName evidence="10">Hippocampus abundant transcript 1 protein isoform X1</fullName>
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        <name evidence="10" type="primary">LOC4811876</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
        <source>
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        <subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="GH21943P-RELATED"/>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="663"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="270"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001352735.4"/>
        </source>
    </evidence>
    <sequence length="723" mass="78210" checksum="E472F7300F0F9DA5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBA7</accession>
    <name>A0A6I8UBA7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial</fullName>
            <ecNumber evidence="7">2.3.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Aminoacetone synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glycine acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Gcat</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>glycine + acetyl-CoA = (2S)-2-amino-3-oxobutanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:20736"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78948"/>
            <dbReference type="EC" id="2.3.1.29"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:20738"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.29" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001352781.3">
        <property type="nucleotide sequence ID" value="XM_001352745.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBA7"/>
    <dbReference type="FunCoup" id="A0A6I8UBA7">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812179"/>
    <dbReference type="InParanoid" id="A0A6I8UBA7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBA7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008890">
        <property type="term" value="F:glycine C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000004">
        <property type="entry name" value="2-amino-3-ketobutyrate coenzyme A ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00985">
        <property type="entry name" value="2am3keto_CoA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011282">
        <property type="entry name" value="2am3keto_CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01822">
        <property type="entry name" value="2am3keto_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005394">
        <property type="entry name" value="PRK06939.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF102">
        <property type="entry name" value="2-AMINO-3-KETOBUTYRATE COENZYME A LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="66"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001352781.3"/>
        </source>
    </evidence>
    <sequence length="417" mass="45567" checksum="58A187D309BBBD59" modified="2020-08-12" version="1">MSTFSKIFLLNGAGGRHYSAAAQTQLREILGSQLAGIKEAGTFKAERIITSSQSTQITVQGSDQRILNFCANNYLGLANNPEIVEYSRKLLEKYGAGLSSVRFICGTQDIHKQLERKIAQFHGREDTILYASCFDANAGIFEAILTPEDAVFSDELNHASIIDGIRLCKAKKQRYKHRDLADLERQLRESDGRLKLIATDGVFSMDGNIAPLARIVDLAKKYNALVFVDECHATGFFGATGRGTEEYDNVMGEVDIINSTLGKALGGASGGYTTGPSELISFLRQKSRPYLFSNTLPPAVVAVGLKVMDMLLASSELTQRVQSNTQHFRTAMTKAGFTIAGENHPICPVMLGDARLASNFADQMLTRGIYVIGFSYPVVPQGKARIRVQISAAHTEAEIDRAIDAFIEVGRSLKVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCI9</accession>
    <name>A0A6I8UCI9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein KRI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4813118</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353348.3">
        <property type="nucleotide sequence ID" value="XM_001353312.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCI9"/>
    <dbReference type="FunCoup" id="A0A6I8UCI9">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813118"/>
    <dbReference type="InParanoid" id="A0A6I8UCI9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCI9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="4">
        <location>
            <begin position="499"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="312"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="588"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="611"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="633"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="654"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="685"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="706"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="724"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="738"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="789"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="828"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="858"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001353348.3"/>
        </source>
    </evidence>
    <sequence length="871" mass="100755" checksum="F4508D6AC6DB7C87" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDF8</accession>
    <name>A0A6I8UDF8_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Homeobox protein Nkx-6.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HGTX</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="term" value="P:animal organ development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:cell differentiation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="homeodomain"/>
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        <property type="entry name" value="NK6 homeobox 1"/>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HD"/>
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        <property type="entry name" value="HD_metazoa"/>
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        <property type="entry name" value="Homeobox_CS"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="HGTX, ISOFORM C"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
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        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
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    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="375"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="377"/>
            <end position="436"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="213"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="360"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="432"/>
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        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="166"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="225"/>
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        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
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        <location>
            <begin position="485"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="RuleBase" id="RU000682"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50071"/>
        </source>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001353698.4"/>
        </source>
    </evidence>
    <sequence length="500" mass="53420" checksum="68EAB02174DBBC2B" modified="2020-08-12" version="1">MDLINPYMRHHHAMAAADYQQQLADYHSAAAAAAVYANNNNNSSNSNNHNHNSRPLMLLKAAAHGGGLKSSSAEDSPSTTPPPASSRLHSESSPSPRYEHNSSPGVDSAKSFALSGGEEQCQNSESASPPPPLDYSPENLTSRGKFHHHHSVNPLALHNHNHTHSGHLNNNHSGHLNNNSINSNSMGMEHKLPLSFLGPPLAALHSMTTEMKTAQGGSGVGASGGPSAAGNGLSYGHSPNAHLISDRGSGGSSSSSSTTATTTNSQGAPNPHGIDTILSKPPPVTSAGLSALTGAGIPRFSIAAAAAGMAQYLSQSQGAPLKTHAGHIVDRTHLYWPGLQGLVANPIAWRERLSNTMSANLSQSHQHHPSNDKDGKKKHTRPTFSGQQIFALEKTFEQTKYLAGPERAKLAYALGMSESQVKVWFQNRRTKWRKRHAAEMATAKRKQDDMGGDNDGDCSENMDSDNESLDMGETPAQSKRCRSNSSSQQQQQQQQDNYRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEN0</accession>
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        <submittedName>
            <fullName evidence="11">Uncharacterized protein isoform X1</fullName>
        </submittedName>
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        <name evidence="11" type="primary">LOC4814078</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001354216.4"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="zinc finger protein 883-like"/>
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    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
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    </dbReference>
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        <property type="entry name" value="Zinc_finger"/>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="ZINC FINGER PROTEIN 154-LIKE"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="886"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="939"/>
            <end position="966"/>
        </location>
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        <location>
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        <location>
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            <end position="1021"/>
        </location>
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        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
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        <location>
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            <begin position="674"/>
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    </feature>
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        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001354252.4"/>
        </source>
    </evidence>
    <sequence length="1157" mass="125377" checksum="BEFAC3F0F233B35E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF16</accession>
    <name>A0A6I8UF16_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Breast cancer metastasis-suppressor 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Brms1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BRMS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354438.3">
        <property type="nucleotide sequence ID" value="XM_001354402.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UF16"/>
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        <property type="interactions" value="704"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814233"/>
    <dbReference type="InParanoid" id="A0A6I8UF16"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000002">
        <property type="entry name" value="breast cancer metastasis-suppressor 1-like protein-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354438.3"/>
        </source>
    </evidence>
    <sequence length="272" mass="30595" checksum="A1C655F0CEAA7AA0" modified="2020-08-12" version="1">MPVKNGDSDGEGDVSGAESERSNSSQGRDSSDEEENNEIDSDDSSEMDSSDIERIRAEHIEDMLSLERQFNTLREQYYFERITWIESQLAEVRSGRSEEFVHPQRDLDKVYRTRIEVADVLRRFRLQNIEHKFLSEEQACVQHLESEKHMAGDNLREELLERIRRLEEDRHNVDISWADWGTDKRQSKVRGPGRKKAVTVTGPYVVYMLREEDIMEDWTIIRKALKRTSTTTSSGPVAPTSGTSVLGGGGPTVSMGLSALSGGVPAMAGASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGY1</accession>
    <name>A0A6I8UGY1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein Ir11a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ir11a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355361.4">
        <property type="nucleotide sequence ID" value="XM_001355325.4"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8UGY1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815841"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056198">
        <property type="entry name" value="LBD_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF37">
        <property type="entry name" value="IONOTROPIC RECEPTOR 11A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24061">
        <property type="entry name" value="LBD_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="580"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ionotropic receptor ligand binding" evidence="10">
        <location>
            <begin position="12"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 3/N-terminal" evidence="9">
        <location>
            <begin position="241"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24061"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355361.4"/>
        </source>
    </evidence>
    <sequence length="614" mass="70972" checksum="42B8440759403CC8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH06</accession>
    <name>A0A6I8UH06_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4815509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355391.3">
        <property type="nucleotide sequence ID" value="XM_001355355.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UH06"/>
    <dbReference type="KEGG" id="dpo:4815509"/>
    <dbReference type="InParanoid" id="A0A6I8UH06"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UH06">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="329"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355391.3"/>
        </source>
    </evidence>
    <sequence length="435" mass="48194" checksum="04FA53F69D7EC973" modified="2020-08-12" version="1">MEQGGDKIYDDFFNGLRSNNGPRNLSQIFEDDVKPLQNESRNMRYQPSSVLKHQQRQQHKIENKEKPKSALSQKWNVEPGVWTTVIAKVAHAFSGSNNMGRVGVALSLHGDGIAKLILYKTKSNVLASVVLTEATASVIERESYIQFYADDKRFWSLRFDLDADEQDIIKALRKYDLPLTCDSAASSCSSSSISDQDNKPEANVKKKSTTPQLQLQPPQPQPRSRLGPNVSKTHRPSEERPLADDVLMTPVPRAIGLSGSGNLLPVRPNEEEIHESLIVSKRLGDSGNEMDKKMDTILQAMHLLVSGASSSTQLNEKHQITMVAGSENSHESEDELIELEQKLLNLKRENRTLVKSLRAKEQALEELRTTSSALGEQNKELKQQNTALLAAMASKAYEQANSTSTSCLNCEQSASLISVLKRQVAALQSAMETTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UI15</accession>
    <name>A0A6I8UI15_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4816230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355851.4">
        <property type="nucleotide sequence ID" value="XM_001355815.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UI15"/>
    <dbReference type="FunCoup" id="A0A6I8UI15">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816230"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355851.4"/>
        </source>
    </evidence>
    <sequence length="789" mass="83706" checksum="20674227BBA1858D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJG6</accession>
    <name>A0A6I8UJG6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Platelet binding protein GspB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356504.4">
        <property type="nucleotide sequence ID" value="XM_001356468.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJG6"/>
    <dbReference type="KEGG" id="dpo:4817244"/>
    <dbReference type="InParanoid" id="A0A6I8UJG6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356504.4"/>
        </source>
    </evidence>
    <sequence length="401" mass="43763" checksum="4F38356A622030AA" modified="2020-08-12" version="1">MVNMSDIDYEPFNPFNVGPPNSGKRMEFKIPTAVTYPPPVFAVVPQDRQGYIRRSVSQDSQLAASSHLKIGPSRIGEVSVSRAIKEEMAAAKRQAASLDEEERIDAGGISKMKLASSRSGIDSKSKVSVKTLMPVRTRRQVAPGDDQAKNRSISGSSVSKNGSRVSWGRETKSRSSSPSKRSSIGAKRSSTRSQSSTASSKASLARSSSKLKPRSSTASTVTLTGKVSKQRSRTDSVKPSSSKMRSKEESRVSLKSRTSKRPSDTVSVRSRTSKTGHKTTETASQRYIKAKATSETLSQHSVQSKTKEASRSASKSKTHPSKVSLKTKVKAAAPKMRSAKALAEDTSLVSRAAAITERLKAGNEDNFRRYSSVIHNHSMRVPEKQFREDRMSFWFQDAVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK48</accession>
    <name>A0A6I8UK48_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Uncharacterized protein fusl</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6 7 8" type="primary">fusl</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
            <tissue evidence="4 5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356820.4">
        <property type="nucleotide sequence ID" value="XM_001356784.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236178.1">
        <property type="nucleotide sequence ID" value="XM_033380287.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236179.1">
        <property type="nucleotide sequence ID" value="XM_033380288.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236180.1">
        <property type="nucleotide sequence ID" value="XM_033380289.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236181.1">
        <property type="nucleotide sequence ID" value="XM_033380290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK48"/>
    <dbReference type="KEGG" id="dpo:4817017"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK48">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070073">
        <property type="term" value="P:clustering of voltage-gated calcium channels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007270">
        <property type="term" value="P:neuron-neuron synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032751">
        <property type="entry name" value="Fuseless"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35270">
        <property type="entry name" value="FUSELESS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35270:SF2">
        <property type="entry name" value="FUSELESS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15993">
        <property type="entry name" value="Fuseless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356820.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033236178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033236179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033236180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033236181.1"/>
        </source>
    </evidence>
    <sequence length="468" mass="52915" checksum="90C785652D2F63F2" modified="2020-08-12" version="1">MLTTDMGTAAGPTPIIVSSKPQKSHCQSPALASEQQLLQHHQQQQQHQHPHLPIHTHGHVHGQGHGQTAMHCEKYMPEAPRRNLHELLLELLDMLLSCLVVAPCVIAYWRGTWELMGVFLFPSSLPLSALSSFLIGGVGHFVFTITQNFFKEHIHPDKRRLTYYAVSRLYTAVFGIVCVNMWRGAWMLCDWLTSVDSLIIVSVVTAIALIFLVATRTLRNLGAAPYTVTMDHKTDYFEVDTMFKIPGFHQPGLYVLDTLFSVFVIGSLVVIAWRGVWGIFDLTLFPKDKAKSAWGSLLIGYLTVFVTFLIHPLMRYVCRRISGILKLIICDIYYLMTFFGAVNAWRGIWNLLDVYLYPDNRLMSFWLTHIIPFLLLAALKCSNSILVRGVFIDADGTGAESVDIPINYVRLHFLRERRKKAQSSPSASHPPPHYYLKPEHATLGRSAEKDKEAQSSLIEKPHATVQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKQ6</accession>
    <name>A0A6I8UKQ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Limbic system-associated membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fipi</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357073.3">
        <property type="nucleotide sequence ID" value="XM_001357037.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKQ6"/>
    <dbReference type="FunCoup" id="A0A6I8UKQ6">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817688"/>
    <dbReference type="InParanoid" id="A0A6I8UKQ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20949">
        <property type="entry name" value="IgI_Twitchin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF6">
        <property type="entry name" value="CONTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026204505" evidence="4">
        <location>
            <begin position="20"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="117"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="217"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="313"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001357073.3"/>
        </source>
    </evidence>
    <sequence length="452" mass="51411" checksum="B064A52598B08395" modified="2020-08-12" version="1">MRLIGFMLNLAALTAVVLGNHHESLSLSPAEHSVVRYTNESLIVQCRSPDPDVKLHWKSPKGEIIREHKGRIHIEQTSPEQLKIVFAHIALADKGNWSCEAAEGRLHSKSFDLIVYQKITFTENVSVITVKEGDKDKTILCEVKGEPQPNITWHFNGQPISVDTADGSKYRILADGLLINKVTQSDTGEYTCRAYQVNSIASDMQERTVLMKIEHKPFWTHKQFVSMQYAYINGTASIMCEAQAEPPATFTWHRKQKRLHSNERLYTIEGDHYWSRLTVHVLNASVFDNYRCRATNHLGSIERTKRLEQGEKPPSPITFQLRGFNSNTFDVDVSAPRGPEDRGPMEVNGFRIEYMSELEFKSDAGKWTNARRKDFPFEEGATFLITNLEPDTYYLMRAASRNLAGFSDFTKVEKYKTLSLEPRVSSAHPLPQSTSLCFGLMALMCLTLRLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL02</accession>
    <name>A0A6I8UL02_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein c(2)M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">c(2)M</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357130.3">
        <property type="nucleotide sequence ID" value="XM_001357094.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL02"/>
    <dbReference type="FunCoup" id="A0A6I8UL02">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817713"/>
    <dbReference type="InParanoid" id="A0A6I8UL02"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF51">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN REC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="4">
        <location>
            <begin position="15"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="295"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001357130.3"/>
        </source>
    </evidence>
    <sequence length="573" mass="64956" checksum="D82200C44F0268E7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULB1</accession>
    <name>A0A6I8ULB1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">beta'COP</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasmic vesicle</location>
            <location evidence="1 12">COPI-coated vesicle membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="12">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357332.2">
        <property type="nucleotide sequence ID" value="XM_001357296.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULB1"/>
    <dbReference type="FunCoup" id="A0A6I8ULB1">
        <property type="interactions" value="2272"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817970"/>
    <dbReference type="InParanoid" id="A0A6I8ULB1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULB1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000008">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="181"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="225"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="15">
        <location>
            <begin position="320"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="16">
        <location>
            <begin position="596"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="844"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="865"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001357332.2"/>
        </source>
    </evidence>
    <sequence length="915" mass="103144" checksum="027C85DC39A10600" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULB2</accession>
    <name>A0A6I8ULB2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glutaredoxin 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Grx3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357339.2">
        <property type="nucleotide sequence ID" value="XM_001357303.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULB2"/>
    <dbReference type="SMR" id="A0A6I8ULB2"/>
    <dbReference type="FunCoup" id="A0A6I8ULB2">
        <property type="interactions" value="2248"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817908"/>
    <dbReference type="InParanoid" id="A0A6I8ULB2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULB2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02984">
        <property type="entry name" value="TRX_PICOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000012">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="6"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaredoxin" evidence="5">
        <location>
            <begin position="132"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001357339.2"/>
        </source>
    </evidence>
    <sequence length="216" mass="23575" checksum="F3C86E1F8397FC7B" modified="2020-08-12" version="1">MPVLSVSNAEEYQNLINADKTTAALFAADWAEQCAQVKDVLEELSKLLGDKLQFISLNAEQFPEISMKHQIEAVPTVIFFAKGSAVDRVDGVDVAAISSKSKKLAESASSAAATGQSLDDRLKALVNTAPLMIFMKGDRNAPRCGFSKQLIAIVNDTSLPYETFDILSDEEVRQGLKTYSDWPTYPQVYVKGELIGGLDIIKELLANKELETTLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UML4</accession>
    <name>A0A6I8UML4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Programmed cell death 6-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ALiX</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357901.2">
        <property type="nucleotide sequence ID" value="XM_001357864.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UML4"/>
    <dbReference type="FunCoup" id="A0A6I8UML4">
        <property type="interactions" value="1983"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800669"/>
    <dbReference type="InParanoid" id="A0A6I8UML4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09240">
        <property type="entry name" value="BRO1_Alix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09235">
        <property type="entry name" value="V_Alix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.280:FF:000001">
        <property type="entry name" value="programmed cell death 6-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF39">
        <property type="entry name" value="PROGRAMMED CELL DEATH 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="3">
        <location>
            <begin position="3"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="710"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="802"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="739"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357901.2"/>
        </source>
    </evidence>
    <sequence length="841" mass="93071" checksum="A5C5356E22571C23" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN16</accession>
    <name>A0A6I8UN16_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase D7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GstD11</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358022.4">
        <property type="nucleotide sequence ID" value="XM_001357985.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN16"/>
    <dbReference type="FunCoup" id="A0A6I8UN16">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800816"/>
    <dbReference type="InParanoid" id="A0A6I8UN16"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UN16">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03177">
        <property type="entry name" value="GST_C_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03045">
        <property type="entry name" value="GST_N_Delta_Epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000034">
        <property type="entry name" value="glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000007">
        <property type="entry name" value="Glutathione S-transferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43969:SF2">
        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D11, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="110"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358022.4"/>
        </source>
    </evidence>
    <sequence length="243" mass="27637" checksum="50D8C7D002A5D5FF" modified="2020-08-12" version="1">MSDTKRIVTEFVYCLLLSGGKMSPPVLYYLPPSPPCRSILLLAKMLGLDFELKIVNILEGEQLKPDFVALNPQHCIPTMNDEGLVLWESRAILSYLVAAYGKSDELYPTDIRVRALVDQRLQFDLGTLYMRLTDYYFPTMFIGAPLDEGKRAKLSEAVGWLNTILEGRQFAAAEHFTIADLTLLVTISQLEAFDFELRPYKHIRQWLERCKDHMAPYDYEELNGSKAVLMGDMFKAKINQGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN97</accession>
    <name>A0A6I8UN97_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Mucin-5AC isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">jvl</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358222.4">
        <property type="nucleotide sequence ID" value="XM_001358185.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232367.1">
        <property type="nucleotide sequence ID" value="XM_033376476.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801060"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UN97">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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        </location>
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        <source>
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    <sequence length="1320" mass="141511" checksum="366815B6470DF00B" modified="2020-08-12" version="1"> </sequence>
</entry>
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                <consortium name="RefSeq"/>
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        </source>
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            <taxon>Diptera</taxon>
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            <taxon>Sophophora</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">MV2-25</strain>
        </source>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        </source>
    </reference>
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        <ligand>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001358411.3"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPP5</accession>
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        <submittedName>
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    <gene>
        <name evidence="11" type="primary">Dic2</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358894.2">
        <property type="nucleotide sequence ID" value="XM_001358857.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPP5"/>
    <dbReference type="FunCoup" id="A0A6I8UPP5">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="GeneID" id="4801865"/>
    <dbReference type="KEGG" id="dpo:4801865"/>
    <dbReference type="InParanoid" id="A0A6I8UPP5"/>
    <dbReference type="OMA" id="MMDTSTM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078116">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="13"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="100"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="199"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358894.2"/>
        </source>
    </evidence>
    <sequence length="299" mass="32913" checksum="28E375B2E50AD2AA" modified="2020-08-12" version="1">MSARRTVLLSYDKRNVARWYFGGVASSMAAMVTHPLDLMKVLMQTQAEKLSVGSTFKKIIREQGVLALYNGISASLLRQYTYTLARFGIYQMGSGMMDTSTMARKTFLAAVAGGIGGFVGAPADLVNVRLQNDVKLPPEQRRNYKQAIDGLIRITREEGWRSMFNGASMTALRGMLMTVGQIAFYEQSKDVLVGLGMPQSTSTYITASLISAAAATTLTQPIDVVKTRRMNARPGEYSGLTDIFVKTTLEGPMAFFKGYTPAFARLMPHTVLLFLTLEFLRINFGYLPEPKPSTTPPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPP9</accession>
    <name>A0A6I8UPP9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA:m(4)X modification enzyme TRM13</fullName>
            <ecNumber evidence="12">2.1.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4801879</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>adenosine(4) in tRNA(His) + S-adenosyl-L-methionine = 2'-O-methyladenosine(4) in tRNA(His) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10401"/>
            <dbReference type="Rhea" id="RHEA-COMP:10402"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74477"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>cytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10399"/>
            <dbReference type="Rhea" id="RHEA-COMP:10400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>cytidine(4) in tRNA(Pro) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Pro) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32767"/>
            <dbReference type="Rhea" id="RHEA-COMP:10397"/>
            <dbReference type="Rhea" id="RHEA-COMP:10398"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the methyltransferase TRM13 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.225" evidence="12"/>
    <dbReference type="RefSeq" id="XP_001358902.2">
        <property type="nucleotide sequence ID" value="XM_001358865.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPP9"/>
    <dbReference type="FunCoup" id="A0A6I8UPP9">
        <property type="interactions" value="1545"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801879"/>
    <dbReference type="InParanoid" id="A0A6I8UPP9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007871">
        <property type="entry name" value="Methyltransferase_TRM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039044">
        <property type="entry name" value="Trm13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021721">
        <property type="entry name" value="Znf_CCCH-type_TRM13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998:SF0">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05206">
        <property type="entry name" value="TRM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11722">
        <property type="entry name" value="zf-TRM13_CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="13">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358902.2"/>
        </source>
    </evidence>
    <sequence length="425" mass="47804" checksum="5CB119DA66A9EA41" modified="2020-08-12" version="1">MAAKKPKMTQKDDVAAVTTCTYWVPRKKRHCKMAANKESLFCGAHSPATEESKAVGAAAERIPCPLDPKHTVFKRKLAKHLTICNARDQASTLPYIVKNINAGEELENPLEDEINSKLRLHELEDTEFYSVIDKVRALYDNHVANSIEELHLQHASLEEPLGHEEYGQESRKHLVQASALLGILEHDQQLTDSTSFIEFGAGKGQLAFFLATALETQGLSHSQVVLIDRMSLRHKKDNKVTNKELVQRIRADIGDLKISALPELKQTKRNVALSKHLCGAATDLTLRCVLAHRTAPTDYILIALCCHHRCSWRSYVGQTWLQAAGLGHREFAIITKMVSWAVCGTGVSRERRKAMAEAHEELGEPPTQRLTREQREQIGYQCKRVLDQGRLEHLRSNGFDASLKFYVPREITLENVVLIAKPMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPZ1</accession>
    <name>A0A6I8UPZ1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CG14292-PA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4801829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358862.2">
        <property type="nucleotide sequence ID" value="XM_001358825.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPZ1"/>
    <dbReference type="FunCoup" id="A0A6I8UPZ1">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801829"/>
    <dbReference type="InParanoid" id="A0A6I8UPZ1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026068430" description="CG14292-PA" evidence="1">
        <location>
            <begin position="20"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358862.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="16880" checksum="13CDA0F1700190F7" modified="2020-08-12" version="1">MKFAIVLLLASIACANSQAAPSAGGGIAINPFAAANPYAAAFNPFLNGLFGGAGAGAGGAGAPVAATPPFGGASISSFFQSVVLQREAERLLSQPNFPTDLAERVQDVVANSQETIAGCSTPATLPWLQIRCVKPLLTSAKNELKLIDDEWQARQTAAASPAAPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPZ9</accession>
    <name>A0A6I8UPZ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription factor castor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cas</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359045.3">
        <property type="nucleotide sequence ID" value="XM_001359008.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPZ9"/>
    <dbReference type="FunCoup" id="A0A6I8UPZ9">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802052"/>
    <dbReference type="InParanoid" id="A0A6I8UPZ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040373">
        <property type="entry name" value="CASZ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451">
        <property type="entry name" value="TRANSCRIPTION FACTOR CASTOR PROTEIN MING -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN CASTOR HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="481"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="540"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="317"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="618"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="647"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="713"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359045.3"/>
        </source>
    </evidence>
    <sequence length="829" mass="92170" checksum="77446727C522FD03" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ55</accession>
    <name>A0A6I8UQ55_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spermidine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SpdS</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359112.3">
        <property type="nucleotide sequence ID" value="XM_001359075.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ55"/>
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        <property type="interactions" value="1291"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802132"/>
    <dbReference type="InParanoid" id="A0A6I8UQ55"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="6"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359112.3"/>
        </source>
    </evidence>
    <sequence length="292" mass="33020" checksum="AE9763F20EF141E2" modified="2020-08-12" version="1">MDTISCGWFSEIQAELWPGQAFSLKVKNVIHKEKSKYQDIQIIETETYGKCLILDGIIQCTSRDEFSYQEMIAFLPLFSHPNPKKVLIVGGGDGGVAREVVKHPLVEEVHQIEIDERVVELSKQHLPEMACGFKSEKLKLTIGDGFEYMKKHKNEFDVIITDSSDPIGPAVSLFNESYYKLMKHALKEDGIVCSQGGSFWLDLSYIKKTMSGCKEHFPKVSYAITSVPSYPCGHIGFVIGSLNQNQKFINSVNKIKNFDIEALNLKYYTPEVHDAAFALPRWVQKSLMSDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQV9</accession>
    <name>A0A6I8UQV9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Short-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="4">1.3.8.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Butyryl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4802606</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:24005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43456"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57389"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:86160"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:43457"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001359495.2">
        <property type="nucleotide sequence ID" value="XM_001359458.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQV9"/>
    <dbReference type="FunCoup" id="A0A6I8UQV9">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802606"/>
    <dbReference type="InParanoid" id="A0A6I8UQV9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016937">
        <property type="term" value="F:short-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046359">
        <property type="term" value="P:butyrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01158">
        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0274">FAD</keyword>
    <keyword evidence="5 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="17">
        <location>
            <begin position="34"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="16">
        <location>
            <begin position="150"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="15">
        <location>
            <begin position="256"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001359495.2"/>
        </source>
    </evidence>
    <sequence length="411" mass="44304" checksum="03E85CA93FF14DDC" modified="2020-08-12" version="1">MQQLVRVALTLGQRSGSVWNASRDRGIASLAALSETHQILQKTCRDFANAELAPRARQHDREGLHPAEQIRRLGELGLMAVTVREEYGGSGLDYQAYAIGMEEVARGDAAVSVAMGVNNLFLGAVQLHGSEQQKQDFLVPYTQGEKIAFYALSEPGNGSDASAASTTAKLDGNSYQLNGTKAWISNSKEASGGVVFATIDKTLKHKGITAFLTAKDVPGLSVGKKECKMGMRASSTCQLVLDDVQVPQFQVLGGPGEGFKIAMQSLDCGRIGIAAQATGIAQAALELAVDYSQKRIAFGKQLSRMQLIQQKLADMATRVEVSRLLTWRAAWLKDNGLPITKEAAMAKLHASEAATYCAHQCTQILGGMGYTTDLPAEMYYRNARVTEIYEGTSEIQRIVIANAVLRELGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URH3</accession>
    <name>A0A6I8URH3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">GTPase-activating protein RacGAP84C isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RacGAP84C</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359760.5">
        <property type="nucleotide sequence ID" value="XM_001359723.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URH3"/>
    <dbReference type="KEGG" id="dpo:4802947"/>
    <dbReference type="InParanoid" id="A0A6I8URH3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8URH3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097149">
        <property type="term" value="C:centralspindlin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20821">
        <property type="entry name" value="C1_MgcRacGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04382">
        <property type="entry name" value="RhoGAP_MgcRacGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000033">
        <property type="entry name" value="Rac GTPase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46199">
        <property type="entry name" value="RAC GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46199:SF3">
        <property type="entry name" value="RAC GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="8">
        <location>
            <begin position="68"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="9">
        <location>
            <begin position="129"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359760.5"/>
        </source>
    </evidence>
    <sequence length="363" mass="42233" checksum="66A4FDFFC26E49FD" modified="2020-08-12" version="1">MQSRVGLLREFSSYHDLTEHFRMYRSQSIETLDNAIDMNPSTGDGLSRDGLDDCYTTCSQSHSGLLREHNFKVKSNYFHVGNCVHCEKRIGFGTACLRCRACPLRCHIGCSRQLTVNCIPQPQMSNKRGSLSDYAPTVAPMVPALIVHCVTEIEERGLQQEGLYRLSSTREKCKQLRQKLLRGKWTPHLGSKDTNTLCCCIKEFLRRLVHPLIPIYHRRDFERATSHSNPEEVEEQVYHVMHMLQQAHRDTLAYLMLHWKRVADSPNVRMSINNLALILAPTLFGDLDLTVENVTTWTKVLKELLLLPHVFWVQFLEVQPAPQSSDYTDDDRYTHRQWDHHHNRRWSNVKTYFRSMVNLSTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URJ7</accession>
    <name>A0A6I8URJ7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">carboxylesterase</fullName>
            <ecNumber evidence="6">3.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">alpha-Est2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001359777.4">
        <property type="nucleotide sequence ID" value="XM_001359740.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URJ7"/>
    <dbReference type="FunCoup" id="A0A6I8URJ7">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802964"/>
    <dbReference type="InParanoid" id="A0A6I8URJ7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000092">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="80"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359777.4"/>
        </source>
    </evidence>
    <sequence length="613" mass="70425" checksum="89DD1759FEEDE23C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URQ0</accession>
    <name>A0A6I8URQ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chitin-binding type-4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4803054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359855.3">
        <property type="nucleotide sequence ID" value="XM_001359818.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URQ0"/>
    <dbReference type="KEGG" id="dpo:4803054"/>
    <dbReference type="InParanoid" id="A0A6I8URQ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21113">
        <property type="entry name" value="AGAP001705-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21113:SF14">
        <property type="entry name" value="LP24064P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03067">
        <property type="entry name" value="LPMO_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026338120" description="Chitin-binding type-4 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-4" evidence="2">
        <location>
            <begin position="24"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359855.3"/>
        </source>
    </evidence>
    <sequence length="229" mass="25129" checksum="27E94314C03B9564" modified="2020-08-12" version="1">MKYFKVILTISLSAFCLVGELEGHGMMLDPVGRSSRWRYDSSAPKNYEDNGLYCGGFWKQTENGGRCGLCGDDWSLEQPRPNELGGKYGSGIIVRTFAGLTEAEINVKITANHLGYFSFNICNLDENGSESEECFNKIPLKFSDGNLKYNINTRTGDIPVTLQLPSDLNCGHCVLRWTYTAGNNWGVCEDGTGAMGCGAQENFVNCADISVLSSARNLIHEVPVEVVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US41</accession>
    <name>A0A6I8US41_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">THO complex subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ref1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360055.2">
        <property type="nucleotide sequence ID" value="XM_001360018.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US41"/>
    <dbReference type="FunCoup" id="A0A6I8US41">
        <property type="interactions" value="2052"/>
    </dbReference>
    <dbReference type="GeneID" id="4803290"/>
    <dbReference type="KEGG" id="dpo:4803290"/>
    <dbReference type="InParanoid" id="A0A6I8US41"/>
    <dbReference type="OMA" id="NEFGPIK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070763">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12680">
        <property type="entry name" value="RRM_THOC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000273">
        <property type="entry name" value="THO complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025715">
        <property type="entry name" value="FoP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF82">
        <property type="entry name" value="THO COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13865">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01218">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="122"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="212"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360055.2"/>
        </source>
    </evidence>
    <sequence length="284" mass="28961" checksum="BF099633289A7A63" modified="2020-08-12" version="1">MVDKIEMSLDDIIKSTRTQKKPQGARGGPGGARRPAGQQRFAAGGGPRRGGGAGPSPRKPAPASGAGGVLKGRRSGGAGGGGGGGGAIQKAKFARGDVNSAWKHDMYDGPKRGAPSSGSGPTLLIVNNLDYGVSNSDIKELFNEFGPIKKAAVHYDRSGRSLGTADVIFERRTDALKAIKQYNGVPLDGRPMTIQLAVSDVNALTRPVASGDIKHRVGGGAGAGAPYKRGGGQAGGPRRGGLRRPAPGGGAAKPAGGARRERKAPPTAEELDAELDSYINDMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USH5</accession>
    <name>A0A6I8USH5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA ligase</fullName>
            <ecNumber evidence="15">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">DNAlig1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="15"/>
    <dbReference type="RefSeq" id="XP_001360228.2">
        <property type="nucleotide sequence ID" value="XM_001360191.4"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8USH5">
        <property type="interactions" value="1608"/>
    </dbReference>
    <dbReference type="GeneID" id="4803515"/>
    <dbReference type="KEGG" id="dpo:4803515"/>
    <dbReference type="InParanoid" id="A0A6I8USH5"/>
    <dbReference type="OMA" id="WIKYKRD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078997">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:1903461">
        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
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        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
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        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
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    </dbReference>
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        <property type="entry name" value="DNA_LIGASE_A1"/>
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    </dbReference>
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        <property type="entry name" value="DNA_LIGASE_A3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 15 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="18">
        <location>
            <begin position="472"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001360228.2"/>
        </source>
    </evidence>
    <sequence length="744" mass="84489" checksum="89C36CEE5C0FBB80" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTD2</accession>
    <name>A0A6I8UTD2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4803813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360471.3">
        <property type="nucleotide sequence ID" value="XM_001360434.4"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:4803813"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001360471.3"/>
        </source>
    </evidence>
    <sequence length="164" mass="19191" checksum="8D8A3CFA526C9EBC" modified="2020-08-12" version="1">MYSLPKQVSLGTQTIPQPAPKPICVPLLMVDEDGKKRYCYHGGKCRCETCEKMREQQADELDAQLLAVLPHSRLKLEPALHQVRPKQYRLEAFYAKPELAEYLKKDHEELRKKYRTYYLPDKYYEKNAWRIPGPQQKQTQTDVRIGNYGIDGCPCVEKSLCWDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTM3</accession>
    <name>A0A6I8UTM3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PTP-ER</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001360590.2">
        <property type="nucleotide sequence ID" value="XM_001360553.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UTM3">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803952"/>
    <dbReference type="InParanoid" id="A0A6I8UTM3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008356">
        <property type="entry name" value="Tyr_Pase_KIM-con"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46198">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46198:SF4">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="923"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
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        <location>
            <begin position="991"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1036"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360797.3"/>
        </source>
    </evidence>
    <sequence length="1048" mass="119606" checksum="5B4D5608A7AC28AB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUG1</accession>
    <name>A0A6I8UUG1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RpLP0-like</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361102.2">
        <property type="nucleotide sequence ID" value="XM_001361065.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUG1"/>
    <dbReference type="FunCoup" id="A0A6I8UUG1">
        <property type="interactions" value="1923"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804560"/>
    <dbReference type="InParanoid" id="A0A6I8UUG1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072591">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000005">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000002">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="126"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="222"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361102.2"/>
        </source>
    </evidence>
    <sequence length="252" mass="28999" checksum="99773F7DC8F7397C" modified="2020-08-12" version="1">MPRSKRDKKVSLTKTDRKGLAWKQRFVDDIRFCVGKYPNIFVFQVQNMRNSILKDLRQEWKNNSRIIFGKNRVMQIGLGRTKSEEMEAGLHKLAKRLNGQVGLLFTEKSKEEVLEWAENYWAVEYARSGFIATETVTLPAGPLEDFAHSMEPHLRSLGMPTKLEKGIVTIYSDYTVCEEGKVLTPEQARILKLVGKPMAKFRMTIQCSWTKSDGFQLHVEDGINDETQVDSAVEEDVEEMDEDKDDDEDEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUM4</accession>
    <name>A0A6I8UUM4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Golgin subfamily A member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Golgin245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361047.3">
        <property type="nucleotide sequence ID" value="XM_001361010.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UUM4">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804491"/>
    <dbReference type="InParanoid" id="A0A6I8UUM4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.60">
        <property type="entry name" value="GRIP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19327">
        <property type="entry name" value="GOLGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19327:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01465">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00755">
        <property type="entry name" value="Grip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101283">
        <property type="entry name" value="GRIP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="3">
        <location>
            <begin position="1416"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="167"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="634"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="955"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1072"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1137"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361047.3"/>
        </source>
    </evidence>
    <sequence length="1476" mass="168126" checksum="FF8731AEDCDC84CD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUN2</accession>
    <name>A0A6I8UUN2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein Tsen15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Tsen15</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN15 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361059.3">
        <property type="nucleotide sequence ID" value="XM_001361022.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUN2"/>
    <dbReference type="FunCoup" id="A0A6I8UUN2">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="GeneID" id="4804507"/>
    <dbReference type="KEGG" id="dpo:4804507"/>
    <dbReference type="InParanoid" id="A0A6I8UUN2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075791">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018593">
        <property type="entry name" value="tRNA-endonuc_su_Sen15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09631">
        <property type="entry name" value="Sen15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen15" evidence="3">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361059.3"/>
        </source>
    </evidence>
    <sequence length="109" mass="12686" checksum="27B9B4BA1AA48DFA" modified="2020-08-12" version="1">MLQLRDFQNVCPDDLTAALALQVYNDLKRDERCGHLQPHYDDDLKVFYLRKEDTQAAYIPLLHTKGIHFSLVEAMQDKFGKNNIFLAIVDNTGNILYYQVTEGFSEKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV40</accession>
    <name>A0A6I8UV40_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">WD repeat and HMG-box DNA-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ctf4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361366.2">
        <property type="nucleotide sequence ID" value="XM_001361329.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV40"/>
    <dbReference type="FunCoup" id="A0A6I8UV40">
        <property type="interactions" value="1053"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804877"/>
    <dbReference type="InParanoid" id="A0A6I8UV40"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057646">
        <property type="entry name" value="WD40_WDHD1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022100">
        <property type="entry name" value="WDHD1/CFT4_beta-prop_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048591">
        <property type="entry name" value="WDHD1/CFT4_hel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932:SF10">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20946">
        <property type="entry name" value="Ctf4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12341">
        <property type="entry name" value="Mcl1_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24817">
        <property type="entry name" value="WD40_WDHD1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDHD1 first WD40" evidence="8">
        <location>
            <begin position="9"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="9"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 second beta-propeller" evidence="6">
        <location>
            <begin position="389"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 helical bundle" evidence="7">
        <location>
            <begin position="686"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361366.2"/>
        </source>
    </evidence>
    <sequence length="886" mass="96442" checksum="558A4E5E2C7C9DE7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV98</accession>
    <name>A0A6I8UV98_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bleomycin hydrolase</fullName>
            <ecNumber evidence="4">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4804638</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001361168.3">
        <property type="nucleotide sequence ID" value="XM_001361131.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV98"/>
    <dbReference type="KEGG" id="dpo:4804638"/>
    <dbReference type="InParanoid" id="A0A6I8UV98"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0788">Thiol protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001361168.3"/>
        </source>
    </evidence>
    <sequence length="473" mass="53868" checksum="481F313FE83B63ED" modified="2020-08-12" version="1">MSEQPNMCFSLTIDQLSTWRKQFYNEPRNLIAQNVCTTHDPLRMCLPILPTVVAGPPNKHGVDSEGKTTSGGPAWITTGLDLLRLEFAKSFPSLPVDFEFSAGYLVYWHKLERCNYLLHAVADHLMRGEPVDGRQFRYLMKHVVPDGGNWQMFVNLVKKYGVIPKQCYQTTGRTRQLNVILRSKLREFVSVLHNRFIFDGDGSALPTLIGEMMPQLYKVINIVLGEPPQEFKWTYYDSKKRYQCLEPLTGSTFFKVLVSKDFDLDEMVCLGHDPRLSSSYLHNYEVALSSNMVGGQLQRYNNQSMDVLVPIIVGSLVGGKAVWLVYDLLAHSSKKASTDFPLLFGLEMCTILNKAERMLYKETRHNRVLLLTEVRLDDEDKPLEFRTIAPSTSTTLFPSSSAANLREINHSVEEKAKIPRPKAPFCIKTDWMREYAFEIVVHSSFVPPDVLGAATRKECKELPPWDIMGAFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVR7</accession>
    <name>A0A6I8UVR7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Map60</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Map60</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361574.3">
        <property type="nucleotide sequence ID" value="XM_001361537.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVR7"/>
    <dbReference type="FunCoup" id="A0A6I8UVR7">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805116"/>
    <dbReference type="InParanoid" id="A0A6I8UVR7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361574.3"/>
        </source>
    </evidence>
    <sequence length="437" mass="47472" checksum="58B15275AF49716F" modified="2020-08-12" version="1">MAIQLDKILADIAKEKALHPNNGGASKQEQEDAWGRIGRLNKLSVHESRSIFIVLQKKYEQEKIKGNSTWKLFNVMHQIHQETKAAEKEEDDQMPMDDVEEYEMLEVQEQEEDDESSAHHPTNSTGSASSDGESPGKSHSTGSPEKDEKVYSKTIIDTPRPAFEEKKLLNTMANNTPRATTNNPVSMAAAVLQKKGITVKKTPSSTGAALKSAPTQPQSNSNRATSSSRTTIQLPDSLKRKLSEEYSGLSQKKVAKTQSTKQTANTVAAALAAASVPDPTVTSNVVHTTTAQLMQVKKERENSHSPPPGINIITVPASQQINGAGPTSSTAATVASNSVASTNGYSPHIEELDFKNDIIFDSKLNTNSNAPEIINLGNFDQSLPPTFFKNLCNSSRHEALGLYVANVMNRISTRAAAKLELGILRTILDVQSDELDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWU8</accession>
    <name>A0A6I8UWU8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable transcriptional regulatory protein MMOB1910</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4811826</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001382221.3">
        <property type="nucleotide sequence ID" value="XM_001382184.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWU8"/>
    <dbReference type="FunCoup" id="A0A6I8UWU8">
        <property type="interactions" value="595"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811826"/>
    <dbReference type="InParanoid" id="A0A6I8UWU8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UWU8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.200:FF:000002">
        <property type="entry name" value="Probable transcriptional regulatory protein CLM62_37755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048300">
        <property type="entry name" value="TACO1_YebC-like_2nd/3rd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049083">
        <property type="entry name" value="TACO1_YebC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532:SF0">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20772">
        <property type="entry name" value="TACO1_YebC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="YebC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TACO1/YebC-like N-terminal" evidence="4">
        <location>
            <begin position="29"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="TACO1/YebC-like second and third" evidence="3">
        <location>
            <begin position="108"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001382221.3"/>
        </source>
    </evidence>
    <sequence length="268" mass="30198" checksum="50AF5C69C5360E7D" modified="2020-08-12" version="1">MLRLLKLPVSFVSRNYCNLNSVRCMAGHSKWANIKHTKAKNDEFKSALFAKIARQLRLAIQDGNSADPAINPSLQSEIEKALKQNMPMSTIQKTLNQFKRVAQNVKKHRLDIRFKRNIYIICTICTDNFAAVKMDAAAMVKKSGGEYIDVGHMFQDCCLIQSQYDTTKLLEGQSLEDRAVEDAIELGAEDIQIVDITTGSINFFCDPDNVFSLSKALANVGYSIENCEHLYVSNNLVTLSLNEKTAYDAFKEKLRNIPGLEDIYDNVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXI3</accession>
    <name>A0A6I8UXI3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BTB/POZ domain-containing protein 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132226.2">
        <property type="nucleotide sequence ID" value="XM_002132190.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXI3"/>
    <dbReference type="KEGG" id="dpo:6903345"/>
    <dbReference type="InParanoid" id="A0A6I8UXI3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048512">
        <property type="term" value="P:circadian behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052407">
        <property type="entry name" value="BTB_POZ_domain_cont_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="43"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132226.2"/>
        </source>
    </evidence>
    <sequence length="380" mass="43587" checksum="95107A0C46F33025" modified="2020-08-12" version="1">MSSNSSKSAKKQSCPKEVHIEDVRLGDSLMEDMGRRCMSEVFADVWFCVEDQRLPAHCVILAARSDFFRELLQGSMPKDRQVPLEVALAPFKVILAYIYTGTLSISTLPLVAIVDVLGVARLYGLEKVEMVLNKRLEQSLHLNNVFTVLGAARRNSLEDLAERCFQFMDRMASELLKEESFLMVSKATLAEVLTRDTFVVSEEAIFSIVCEWIGRHPMENIQSLFSLVGLPLTSEEDLVVRPASRGHVEPENIPKFVPCHTTLWPEKDVAFDKYFLRRISGSHNEPMIDLRHCYRINFISVIERLKSAEICYDVEVSCDRNRWDRVGRMMPGGMPLPDVWINIYFALRPIRFIRIVNPENGRKDLLNYFELKAKHTTKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY89</accession>
    <accession>B5DJR6</accession>
    <name>A0A6I8UY89_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-pyruvoyl tetrahydrobiopterin synthase</fullName>
            <ecNumber evidence="4">4.2.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">pr</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTPS family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_002133137.1">
        <property type="nucleotide sequence ID" value="XM_002133101.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236187.1">
        <property type="nucleotide sequence ID" value="XM_033380296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY89"/>
    <dbReference type="GeneID" id="6902611"/>
    <dbReference type="KEGG" id="dpo:6902611"/>
    <dbReference type="OMA" id="HFNAAHK"/>
    <dbReference type="UniPathway" id="UPA00849">
        <property type="reaction ID" value="UER00819"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250370">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003874">
        <property type="term" value="F:6-pyruvoyltetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00470">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.10:FF:000003">
        <property type="entry name" value="6-pyruvoyl tetrahydrobiopterin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022470">
        <property type="entry name" value="PTPS_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022469">
        <property type="entry name" value="PTPS_His_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00039">
        <property type="entry name" value="6PTHBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00987">
        <property type="entry name" value="PTPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00988">
        <property type="entry name" value="PTPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002133137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033236187.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="19201" checksum="896D84D6A3F50AD7" modified="2020-08-12" version="1">MSQQPVAFLTRRETFSACHRLHSPQLTDAENLEVFGKCNNFNGHGHNYTVEITVRGPIDQRTGMVLNITELKDAIEAVIMKNLDHKNLDKDVEYFAKTPSTTENLAVYIWNNIRLQLKKPELLYEVKIHETPKNIISYRGPYALNGIYNPINKRIAHDSCTNISSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ46</accession>
    <name>A0A6I8UZ46_DROPS</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6902313</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133840.3">
        <property type="nucleotide sequence ID" value="XM_002133804.3"/>
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        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902313"/>
    <dbReference type="InParanoid" id="A0A6I8UZ46"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ46">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000003">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 10 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039919">
        <property type="entry name" value="ARHGEF10/ARHGEF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877:SF7">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 10-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19057">
        <property type="entry name" value="PH_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="1034"/>
            <end position="1221"/>
        </location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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    </feature>
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        <location>
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        </location>
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        </location>
    </feature>
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    </feature>
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            <begin position="2306"/>
            <end position="2321"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002133840.3"/>
        </source>
    </evidence>
    <sequence length="2345" mass="250527" checksum="E7F1AE3C642BF1A1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ74</accession>
    <name>A0A6I8UZ74_DROPS</name>
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        <submittedName>
            <fullName evidence="6">GTP-binding nuclear protein Ran-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6902410</name>
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    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:protein import into nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Small_GTPase"/>
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    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
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        <property type="entry name" value="GTPRANTC4"/>
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    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
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    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002133344.3"/>
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    <sequence length="182" mass="20758" checksum="B2E98AD0672AAA73" modified="2020-08-12" version="1">MDVPTYKCIIIGEKRCGKTTFLRRHLTGEFKSEYFPTDKNSVNIDTLIFSSNRGPIRFDVWDWGKEDEELGRYPDDFYSNSHCAIIMFDASSVDLFQNFHKFMTLNKICPGIPISICGNKSDIKPGSDFPLNPAHRLCKISVKTGLNMDGPFLYLARKLFNDFTLEFVKLPPLVFPQPGCVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZY2</accession>
    <name>A0A6I8UZY2_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC6901732</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134401.2">
        <property type="nucleotide sequence ID" value="XM_002134365.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZY2"/>
    <dbReference type="KEGG" id="dpo:6901732"/>
    <dbReference type="InParanoid" id="A0A6I8UZY2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZY2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031750">
        <property type="entry name" value="DUF4734"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22667">
        <property type="entry name" value="AT01380P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22667:SF0">
        <property type="entry name" value="AT01380P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15881">
        <property type="entry name" value="DUF4734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BACK" evidence="2">
        <location>
            <begin position="170"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="409"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002134401.2"/>
        </source>
    </evidence>
    <sequence length="453" mass="51842" checksum="BF13631B282E214D" modified="2020-08-12" version="1">MFQQNQVKEWQVTRRRITAVNLGNTGIAEQLEAFKKHTAEVEKANGQCSKKFIRLKNKDRLEDVVMIHIESSRSTIVNVDGVRFLCHDAVLSFFSKRLTEQMRKGCLYFETPELASRAFDKIYRWMHGECSVVQSFDLAVLRGARFLAIPELLEQMWKLLEKKNPTEYDAFEMLCKSRKVHEIEELHGMMVDRITKSTLVIFSSKQFLRLTETQVSLLLKSDRLAVNSEIEVLYSGLCWLRYKWPMRRGSVGKVLNSVRYGFLSVLMLQKVSLADRPQTGPFADILDVFLKLPESKKILDDALIYSSLLATTVMEPHCLKDRLATTGIQLLPSRRWMIDTRCEYHRPVSATIPNMSFVSWKEFKNYMFMLQTEKTNIEDFRCVDELERHICPGSESDLSVAELEPYGSTSSLLSEDTDSGSTTLVASSFNDSSSASESVLTSTEDTEGSFSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1A5</accession>
    <name>A0A6I8V1A5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135616.2">
        <property type="nucleotide sequence ID" value="XM_002135580.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1A5"/>
    <dbReference type="KEGG" id="dpo:6900294"/>
    <dbReference type="InParanoid" id="A0A6I8V1A5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V1A5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135616.2"/>
        </source>
    </evidence>
    <sequence length="148" mass="16586" checksum="CAE5F1D091378129" modified="2020-08-12" version="1">MAIERKVPKSKPRSSVAARRVKKPNPVRLRSLGDLKESEMIKVTSALNAAVNLASAEDDLKKNSDSPFCLPDEAEMWRRKQKMRRRVATAMKLLALVERGYVDSGFEHTAPVTLPEVPVRRRSRSATTATIDAASNCRICGRPMPEQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2B3</accession>
    <name>A0A6I8V2B3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbonic anhydrase</fullName>
            <ecNumber evidence="4">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6896882</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 4">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_002137072.3">
        <property type="nucleotide sequence ID" value="XM_002137036.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2B3"/>
    <dbReference type="FunCoup" id="A0A6I8V2B3">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896882"/>
    <dbReference type="InParanoid" id="A0A6I8V2B3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00326">
        <property type="entry name" value="alpha_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF137">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026378347" description="Carbonic anhydrase" evidence="4">
        <location>
            <begin position="20"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="5">
        <location>
            <begin position="35"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002137072.3"/>
        </source>
    </evidence>
    <sequence length="304" mass="34905" checksum="3DBF95A5BFA743B6" modified="2020-08-12" version="1">MPESRLFQKLLLLLPLAYTNKPQLPHNDDGDHRVHHWNYEYNGNDWGGTCRTGLRQSPIRLAVQKSLLVAMPRITFGNYDTKLRPPLTLVNNGHSAHMDIPETLDNSRPFITGGLLKGRYIAEGLHFHWGSPNWRGSEHSIQDHRYDVEMHIVHRNSRYADLPEALKYKDGVAVLGVMFKIVQTPDAFFPGLNKIFAQLPKIKKYKEEATILGSLTLGQVFGNLNTRDFFTYRGSLTTPDCEEAVTWTVFSQPLPISFPAVSRFWNLRSSDGYRLVNNYRNIQPRNNRPVYYRTSPNQINNLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2L6</accession>
    <name>A0A6I8V2L6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Acyl-CoA Delta(11) desaturase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6897154</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The histidine box domains are involved in binding the catalytic metal ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137334.2">
        <property type="nucleotide sequence ID" value="XM_002137298.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2L6"/>
    <dbReference type="KEGG" id="dpo:6897154"/>
    <dbReference type="InParanoid" id="A0A6I8V2L6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001522">
        <property type="entry name" value="FADS-1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00476">
        <property type="entry name" value="FATTY_ACID_DESATUR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="15">
        <location>
            <begin position="75"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002137334.2"/>
        </source>
    </evidence>
    <sequence length="362" mass="41993" checksum="56B8A38240EC985A" modified="2020-08-12" version="1">MPPNSEVVEFGPKVSDTTGVLYEMDAEPEGTAKDLSTLKTTDGRKLELVWMNIVLFIYVHTASVYGIWLLLTAIKWQTFVFSVILFLFSTVGISGGAHRLWAHRTFKANLPLRLILLFFNTLAFQDAVYNWSRDHRLHHKYSETDADPYNSRRGWFFSHMGWLCCKKHPNVTAKGKLIDLSDLRRDPLVMFQKKHYLILMPICCFILPTLVPVYFWGESLSVAWHVPALLRWCLVLNGVWLLNSSAHLYGKRPYDQSISPTNQSFLIWLRMGEGYHNYHHVFPWDYKSAEMGHFSQDLTTNFIKTFARLGWAYDLKSVSLDMVQKRVLRTGDGTHPIWGWGDKDHPQQDIDSTIITHKKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V353</accession>
    <name>A0A6I8V353_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137735.2">
        <property type="nucleotide sequence ID" value="XM_002137699.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V353"/>
    <dbReference type="KEGG" id="dpo:6897590"/>
    <dbReference type="InParanoid" id="A0A6I8V353"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21148">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00026148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137735.2"/>
        </source>
    </evidence>
    <sequence length="143" mass="16372" checksum="87145B649EFDF346" modified="2020-08-12" version="1">MKAENNKKDWLANGHGVYTQLFDEKEFFEMASHTPNIVVLFYQTGNQGCRIMDWHLKLLAAKHLEAKFCKLNIERAGFLQKRLRIEVIPEILLVKDSTTADFIVGFQDVNFSTEMLECRIARSGTISYQGDLSKSSDVKRILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3B9</accession>
    <name>A0A6I8V3B9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Probable cytochrome P450 313a3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6897665</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137803.2">
        <property type="nucleotide sequence ID" value="XM_002137767.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3B9"/>
    <dbReference type="FunCoup" id="A0A6I8V3B9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897665"/>
    <dbReference type="InParanoid" id="A0A6I8V3B9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11057">
        <property type="entry name" value="CYP313-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF50">
        <property type="entry name" value="BIFUNCTIONAL ALBAFLAVENONE MONOOXYGENASE_TERPENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002137803.2"/>
        </source>
    </evidence>
    <sequence length="493" mass="56945" checksum="BCFEF9D41CEEDF71" modified="2020-08-12" version="1">MYSLHLWIAVGILAWIYFLWSRRRFYLLMLRVKGPVGYPFLGVAVEYLRNKHNMEQRSKQFQEYGPTNLTWIGSVPVLVTCEPNVVQDVLTSPHCLDKSKIACNPLRLICGPATFALNGSHWVERRKQMNPSFRHNVLMSFFPLFNEETKNLISLLETFVGQGEKKLLPEFINWSFRTSIQSTMGSDLKDNPSIMNGAIQKKISNLIEVTYHLITFPWLQNRAISTLFGFEKRKSEAMIHLNASLQSILQEKLKTQGDESKESLASRNIVINRAMDFLRSGDFSYEDVKSECILTIVAGFETSAITVFNTLILLAMFPEYQDTAFEELKDVFPNAGDFEVSYDDLQKLVYMDRVLNESLRLLPPLPLIPREVTEDLKLSNGILIPKGVTVAIDIFNTHRNKDFWGLEADTFNPDNFLPEHIQERHPYVFIPFNRGKRNCAGRKYAEISIKIALAKILRNFRLSTSFRFDDLSFVDNIVLEFKEQPLLELQHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3F7</accession>
    <name>A0A6I8V3F7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6897604</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_002137749.3">
        <property type="nucleotide sequence ID" value="XM_002137713.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3F7"/>
    <dbReference type="KEGG" id="dpo:6897604"/>
    <dbReference type="InParanoid" id="A0A6I8V3F7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000596">
        <property type="entry name" value="CK1 family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="137"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137749.3"/>
        </source>
    </evidence>
    <sequence length="473" mass="55018" checksum="508DB100C5275DDC" modified="2020-08-12" version="1">MAEIAIVSRRSDESSPLSMTYSEADDATYTSITITAEEVRSPSLNSEDNLSSLDETKTLVAAENHTHQCHKADGFFYTGNSGKRKFSPNSIVLAWKKHTHEFIKKEKKLKKRRYRRPQGTPKVDLMDPSELIVARKYRLIERIGNGSFGELYQAVDIKKGKEVAVKVEYITAAHSMLEREAKVYKLLAGTEGIPKIRYYGSERDVNVLVLDLLGPSLEDLLNFCTRTFTLKTTLMLALQMLTRLECVHKKGYIHRDIKPDNFLMGLNGRRCLVFLIDFGLAKKYYDPHTGKHIEYCQKRELVGTPRYSSVRSHFAEQGRRDDLESLGYMLIYFRHGRLPWQGVRADTRAQKFEQIGEMKASIKLDSLCNQMPEEFLLFLRYCRCLHFGDIPDYVYLRRMLQSILFRKGLLNDFLFDWVDPKHLAIHQRDCVDQRGRQITKNNVNCCSCSYHKYKRRLDHQRDRNREPHPTCPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3H2</accession>
    <name>A0A6I8V3H2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17 18">Zinc finger and SCAN domain-containing protein 12-like isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="16">zinc finger and SCAN domain-containing protein 12 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17 18" type="primary">LOC6897940</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17 18">MV-25-SWS-2005</strain>
            <strain evidence="15 16">MV2-25</strain>
            <tissue evidence="16 17 18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138043.2">
        <property type="nucleotide sequence ID" value="XM_002138007.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038317.1">
        <property type="nucleotide sequence ID" value="XM_015182831.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236826.1">
        <property type="nucleotide sequence ID" value="XM_033380935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3H2"/>
    <dbReference type="GeneID" id="6897940"/>
    <dbReference type="KEGG" id="dpo:6897940"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247545">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V3H2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF66">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="13">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="ZAD" evidence="14">
        <location>
            <begin position="152"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="562"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="590"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="86"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="351"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002138043.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015038317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033236826.1"/>
        </source>
    </evidence>
    <sequence length="630" mass="73114" checksum="E50C72DB04C5C4F3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3M6</accession>
    <name>A0A6I8V3M6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138080.2">
        <property type="nucleotide sequence ID" value="XM_002138044.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:6897981"/>
    <dbReference type="InParanoid" id="A0A6I8V3M6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006623">
        <property type="entry name" value="THEG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14912">
        <property type="entry name" value="THEG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00705">
        <property type="entry name" value="THEG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138080.2"/>
        </source>
    </evidence>
    <sequence length="256" mass="29843" checksum="4E80F94748018DFC" modified="2020-08-12" version="1">MVSGINIGDARAAKTVLTIPTDEINQKPVPSWVEWVERNSKPLVRKRHREERILTKWQKKGPMKKKDWKKFYHWATTKAAPQERTFKEAENLYQAGGGKAGGSMDWMDLDEKLVELAKPRIVNKKHQFHDDDSPYNPVITVGQPPHRDRGRPFQPPSVPCCFQHNEVEAEFWSQLRFPVRKEALKGQASPRIIDLARPRTLPPTPHCPIPPKTPRPLDVPPPKRKKFTPQGWRLHQIRLTYLSQPMNRGHYEYFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4Y2</accession>
    <name>A0A6I8V4Y2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein Fs isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Fs</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138138.2">
        <property type="nucleotide sequence ID" value="XM_002138102.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4Y2"/>
    <dbReference type="FunCoup" id="A0A6I8V4Y2">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898052"/>
    <dbReference type="InParanoid" id="A0A6I8V4Y2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000013">
        <property type="entry name" value="Follistatin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000061">
        <property type="entry name" value="Follistatin, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000057">
        <property type="entry name" value="Follistatin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003645">
        <property type="entry name" value="Fol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866:SF29">
        <property type="entry name" value="FOLLISTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866">
        <property type="entry name" value="SPARC OSTEONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21333">
        <property type="entry name" value="FST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00274">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TB" evidence="6">
        <location>
            <begin position="190"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="7">
        <location>
            <begin position="628"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="7">
        <location>
            <begin position="694"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="7">
        <location>
            <begin position="768"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="299"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="403"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="432"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="493"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="518"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002138138.2"/>
        </source>
    </evidence>
    <sequence length="823" mass="90602" checksum="A020616E6C4E2F8C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V687</accession>
    <name>A0A6I8V687_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable transcriptional regulatory protein MS53_0373</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4803258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736933.1">
        <property type="nucleotide sequence ID" value="XM_003736885.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V687"/>
    <dbReference type="FunCoup" id="A0A6I8V687">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="GeneID" id="4803258"/>
    <dbReference type="KEGG" id="dpo:4803258"/>
    <dbReference type="InParanoid" id="A0A6I8V687"/>
    <dbReference type="OMA" id="RWAPMYG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076134">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048300">
        <property type="entry name" value="TACO1_YebC-like_2nd/3rd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532:SF0">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="YebC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TACO1/YebC-like second and third" evidence="2">
        <location>
            <begin position="163"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_003736933.1"/>
        </source>
    </evidence>
    <sequence length="319" mass="36755" checksum="4F912F3942AC64BF" modified="2020-08-12" version="1">MPNKRLPHLNASMQPHMSRPSLVRSSSPKQEPDKRNGLDRDLLEESREECKRVRWNIPFDSLQDKRGPSKSKLSQKSPSFITPSSTKPMQFDPDTPKEVQVAHELRLAVHRGGGSTDPDHNYALRAKLSPLQLPYSRIQRILKECELYERNQNTFIVPIDYNDDQVALVCKARTHNLAELKQRLAPLLMRYKARLYNRHLFTETGVIKAIVPRKTRLNFSDFSQTVQTDALWCKASSGEISDYENGFVVFKCPPNELADVAAKLKQSGYKIHHSEIGHCPNEPLVSLSPRHLKRYVDFVMELKQDEDIFKVYDNVDHQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7G7</accession>
    <name>A0A6I8V7G7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">Dual oxidase maturation factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">mol</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11 12">MV-25-SWS-2005</strain>
            <tissue evidence="10 11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DUOXA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035424.2">
        <property type="nucleotide sequence ID" value="XM_015179938.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035425.2">
        <property type="nucleotide sequence ID" value="XM_015179939.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236490.1">
        <property type="nucleotide sequence ID" value="XM_033380599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V7G7"/>
    <dbReference type="KEGG" id="dpo:6903466"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V7G7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018469">
        <property type="entry name" value="Dual_oxidase_maturation_fac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31158">
        <property type="entry name" value="DUAL OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31158:SF10">
        <property type="entry name" value="LD27791P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10204">
        <property type="entry name" value="DuoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="337"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="337"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015035424.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015035425.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033236490.1"/>
        </source>
    </evidence>
    <sequence length="485" mass="54329" checksum="53331A66E80B76E3" modified="2020-08-12" version="1">MKGWFDAFRDDGGPTLYSFSNRTPVTGDVSIVAVSVLFATFYVAFLVIFPGVRKQKFTTFSTVTLSLFVGLVILITRLGSAWHVAHATIIAPYKAFSREKLPARIGTHIGLMHVNVTLTAIPIGNYTPPDIDYNERFMWEGATDMSANYRHALQRGLPFPILTVAEYFSLGREGFSWGGQYRAAGYFASIMLWSSLASWLLMNLLLIAVPRYGAYMKALTGALLVCTTVGYHCLLPKRPLCIYLEGGRLEFHFGWCYWLVLVAGILCFIAGVLISIIDLVWPHTFSTVLEVYYGTPYDRHVILEESSDVRYRKPRNSRSLEDPPGLGSRILRRLSSKARDMQHVTAPRRDSPAGVSSSAGGGGGGGSGGVENKGFQSDVPKSPWRYPFRRTQQMPPPHQQHQHQHPLQQSHSHQHQHQHHHQQQLQFVGGPVVQHPMHHMQRTMSQDSGSSMGSAAVQISPLHKHALARMLPNPPVERIRDMDHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAJ9</accession>
    <name>A0A6I8VAJ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ninjurin-2 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NijC</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ninjurin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037198.1">
        <property type="nucleotide sequence ID" value="XM_015181712.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAJ9"/>
    <dbReference type="FunCoup" id="A0A6I8VAJ9">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="GeneID" id="4801350"/>
    <dbReference type="InParanoid" id="A0A6I8VAJ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072988">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007007">
        <property type="entry name" value="Ninjurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316:SF17">
        <property type="entry name" value="NINJURIN C, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316">
        <property type="entry name" value="NINJURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04923">
        <property type="entry name" value="Ninjurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015037198.1"/>
        </source>
    </evidence>
    <sequence length="155" mass="17512" checksum="52818DD504E28F98" modified="2020-08-12" version="1">MAQVVAGDERVNETEYLLPLQKSVKYAWQMKAMDANRYATKKTIAQGMLDIALLTANASQLKYILQVGEQHQFYKLMLILISLSIVMQILSGVLSLSLSLLRDCRLHQPEFHQSANVINHVRTAFAFFTTMLNLFISAFDSRLPPPQGDLLNNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAV1</accession>
    <name>A0A6I8VAV1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Calcineurin B homologous protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4801656</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the calcineurin regulatory subunit family. CHP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037343.2">
        <property type="nucleotide sequence ID" value="XM_015181857.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAV1"/>
    <dbReference type="InParanoid" id="A0A6I8VAV1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051875">
        <property type="entry name" value="Calcineurin_B_homologous"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46002">
        <property type="entry name" value="EG:114D9.1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="130"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015037343.2"/>
        </source>
    </evidence>
    <sequence length="233" mass="27134" checksum="6A50C12CBA603097" modified="2020-08-12" version="1">MGGCCCRNPTSPYPTVPMEFMDSLKSKTQRTRVVKVIGESQKVLAPPPVKPTQIQYLYNRFCQISGEYRPNQVQKYNFYSGLLSLNPLLPTILNCMFGDKNVITFVEFAMFLSTFQAHSLKTKRGVKNEGKDRKLNYLFSMYDHNKDGRITKVDLVIIVHKLFAHMMDHLQIMRIVDTMMTEMDCTDTNQITFRDFCKAFEVFDMAEMMVTFIPEFRGRSNDDGKSSKWFCWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDP1</accession>
    <name>A0A6I8VDP1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12 13">Uncharacterized protein koi isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">koi</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015039067.2">
        <property type="nucleotide sequence ID" value="XM_015183581.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039068.2">
        <property type="nucleotide sequence ID" value="XM_015183582.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039069.2">
        <property type="nucleotide sequence ID" value="XM_015183583.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDP1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VDP1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000009">
        <property type="entry name" value="SUN domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="9">
        <location>
            <begin position="815"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015039067.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015039068.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015039069.2"/>
        </source>
    </evidence>
    <sequence length="979" mass="110884" checksum="66AD0C6DBD19628A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VF21</accession>
    <name>A0A6I8VF21_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039928.2">
        <property type="nucleotide sequence ID" value="XM_015184442.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VF21"/>
    <dbReference type="KEGG" id="dpo:26532905"/>
    <dbReference type="InParanoid" id="A0A6I8VF21"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026306397" evidence="1">
        <location>
            <begin position="20"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015039928.2"/>
        </source>
    </evidence>
    <sequence length="198" mass="22804" checksum="82E4D9F5D6FC1301" modified="2020-08-12" version="1">MLAASLCVILLFQVNSISGQIYQINKINLNDNVQAINPYGSHVQIFDSTDHGRPYPWWWEMVKNLTIQSNTTTQAVQVISPWGNGSGIFQLNTIPTQSNVNTPLRTLLKNWFFRQPAQPNIQVLTLGGRALAEKQYLHELNPTHVLLPRRRATAGRRHQQRRILMRNLRSGEVMLMNTQTLRMPFYEDDWDIETSYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJ46</accession>
    <name>A0A6I8VJ46_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042397.2">
        <property type="nucleotide sequence ID" value="XM_015186911.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VJ46"/>
    <dbReference type="KEGG" id="dpo:6901012"/>
    <dbReference type="InParanoid" id="A0A6I8VJ46"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026071080" evidence="2">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015042397.2"/>
        </source>
    </evidence>
    <sequence length="128" mass="14055" checksum="DFDCA8D993966255" modified="2020-08-12" version="1">MKGQTLTSGMLTNLLIVLGNGIGWDIHIPLRSAVAAATKGTVHTRNSTDIELEIRPALWRQLRVYSYSHMQTESPSQSHERSTGETALGKPHWGNRTGETVLGRRISKHQQLLDACCSGALQRSSPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKZ3</accession>
    <name>A0A6I8VKZ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Or67d</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043125.2">
        <property type="nucleotide sequence ID" value="XM_015187639.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VKZ3"/>
    <dbReference type="InParanoid" id="A0A6I8VKZ3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="91"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015043125.2"/>
        </source>
    </evidence>
    <sequence length="440" mass="48657" checksum="D3FC53CB2285E56E" modified="2020-08-12" version="1">MMAYKRWFPREKSHNPSTSLLERKTLWRDHVRGTLRALLQDQPGHSLLRGPVRQRRHCRGLSHVVAHVRGDRGDTVLLRLHRVHGVRGRCAGRRPHRHTAGLRPGGVRRAGPRQAAGHRPDGVGGAPNPGHVRGHLQGVRAAGRRLRQVPGEAHQDHVAHAHVLHVGVRRAGGRADRLPVLPSDPPPQEAAGDAVPGAVDRREHGRGLSGAHLDTRDALVDGRIRQLWRRHVSVPVHQQRAHAEGHLQREAQGVQRGGCAAPGLPKDAHTPVGSARLASAVREVGLGHTFWLHSPSTRSPFPSSILRDTERIYRIVLFVQLSTNCVSILCTISCIFIGAWPAAPIYLVYSFIVMYSFCGLGTIVETSNEDFSKEIYANCLWYELPVKEQRLVILMLAKSQHEISLTAADVMPLSMSTALQLTKGIYSFSMMLITYLGYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMH3</accession>
    <name>A0A6I8VMH3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015044505.2">
        <property type="nucleotide sequence ID" value="XM_015189019.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VMH3"/>
    <dbReference type="KEGG" id="dpo:26533697"/>
    <dbReference type="InParanoid" id="A0A6I8VMH3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015044505.2"/>
        </source>
    </evidence>
    <sequence length="121" mass="13368" checksum="DD31116EB81B46F3" modified="2020-08-12" version="1">MGQDGPDYQPAKAGKAAAKAPSKRQRRKVAKLEEGNGQGRQIILVLNEESVRLLHDTEGTVDYGYKKVIIVEPPEIPSMEIKPVSVSDEASVVSDDILDGWITTKFKNFLQNFLGIYPIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNJ3</accession>
    <name>A0A6I8VNJ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26534306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232597.1">
        <property type="nucleotide sequence ID" value="XM_033376706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNJ3"/>
    <dbReference type="KEGG" id="dpo:26534306"/>
    <dbReference type="InParanoid" id="A0A6I8VNJ3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033232597.1"/>
        </source>
    </evidence>
    <sequence length="108" mass="12465" checksum="86818851BE3F2921" modified="2020-08-12" version="1">MQKTAQDQSKCIAAAAIEDFHQENYDPGAGEETFGQKKALDREEGKLPETQGGTLIQRLGFEELLRRLVWAKNLNQKELETHCKSARYYRTKIYRSARPEEKTFLTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPY5</accession>
    <name>A0A6I8VPY5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233111.1">
        <property type="nucleotide sequence ID" value="XM_033377220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPY5"/>
    <dbReference type="KEGG" id="dpo:117183397"/>
    <dbReference type="InParanoid" id="A0A6I8VPY5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233111.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="19867" checksum="38B5563549528B35" modified="2020-08-12" version="1">MILILDCSDGLFKFKNIKCKCYEKSYCEFAKCELKILGRGIVGLNLHVQAKQLPITRFQAQVTTFQRFNGFRPNMYNISVDICDFFKHKKRFPFFKILHDPLRNFSNFNHSCPYNHDIIINRMVLNDQMLNNLPLPNGFYKLLIVIATEGIWRGKVEFFFEINLGYDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQW4</accession>
    <name>A0A6I8VQW4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PilZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26533904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233446.1">
        <property type="nucleotide sequence ID" value="XM_033377555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQW4"/>
    <dbReference type="KEGG" id="dpo:26533904"/>
    <dbReference type="InParanoid" id="A0A6I8VQW4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19873" checksum="D0FBF2FC7F618D44" modified="2020-08-12" version="1">MDILLLHEYNHFQQQRRLRWDCFDCQTCPQYADHVKCLIADPRQSRLNVEVNLLEAVPSIRTYMKISTRFKPSEAYRKFLDISFDGCRVISDLSKGTTMIAKIYNAVVKFSNQQRKCPIKKGFIYYHNISIEELLPSFLPASSIFVEVHFFHPKKTYLNMTLRGDISEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VT49</accession>
    <name>A0A6I8VT49_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Monocarboxylate transporter 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">chk</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033234250.1">
        <property type="nucleotide sequence ID" value="XM_033378359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VT49"/>
    <dbReference type="FunCoup" id="A0A6I8VT49">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803432"/>
    <dbReference type="InParanoid" id="A0A6I8VT49"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000464">
        <property type="entry name" value="Monocarboxylate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF111">
        <property type="entry name" value="CHASKI, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="688"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="720"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="754"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="780"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="813"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="846"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="680"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033234250.1"/>
        </source>
    </evidence>
    <sequence length="889" mass="97625" checksum="71920964E42D9D0E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VU41</accession>
    <name>A0A6I8VU41_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 3 4 5 6" type="primary">LOC4804075</name>
    </gene>
    <organism evidence="1 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2 3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3 4">MV-25-SWS-2005</strain>
            <strain evidence="1">MV2-25</strain>
            <tissue evidence="2 3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360693.3">
        <property type="nucleotide sequence ID" value="XM_001360656.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039588.2">
        <property type="nucleotide sequence ID" value="XM_015184102.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039589.2">
        <property type="nucleotide sequence ID" value="XM_015184103.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234443.1">
        <property type="nucleotide sequence ID" value="XM_033378552.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234444.1">
        <property type="nucleotide sequence ID" value="XM_033378553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VU41"/>
    <dbReference type="KEGG" id="dpo:4804075"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001360693.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015039588.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015039589.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033234443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033234444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="47053" checksum="CBA4382A1D62CFCE" modified="2020-08-12" version="1">MEFLPDADLISIFGLLDLKSQLNMAKVHSRFYSLMPLVWGSKVSLSLFELEISSGDLRHYLSIIRTKLQVLRLNMINRESFEVLTSYVFPNAHDFRFSTESFHLKDADILMIIKAFPQLRTFSPVGHFTGKYFAYIPHLENLRITYCGGFEGNNLVHIMQAKQLKTLNLDLFGCDEDILDIDLPTDGMKSIEVLICDEEEFSIWFMNNLKHLKNLKQLTIRGKNYPSKLLDIAEKLKAVGRHRLKQLEVHNACNSYFDAERLQLDVETFVLNYVKNLFPSRCNITHPFRHIKRLLFYSCNIEDADCFGHFLQLCRHVDVIAFEDCSFGFQHYTFSAPRIARHRATALNMYLEGTTFVKHFEDGAPMTWSVEGEDRLFKLHLTKSKIHCVGQRNISINFYPAETEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VX75</accession>
    <name>A0A6I8VX75_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Sodium-coupled monocarboxylate transporter 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">LOC6902923</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15">MV-25-SWS-2005</strain>
            <tissue evidence="14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235662.1">
        <property type="nucleotide sequence ID" value="XM_033379771.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235663.1">
        <property type="nucleotide sequence ID" value="XM_033379772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VX75"/>
    <dbReference type="KEGG" id="dpo:6902923"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VX75">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11492">
        <property type="entry name" value="SLC5sbd_NIS-SMVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF41">
        <property type="entry name" value="GH19970P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="518"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033235662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033235663.1"/>
        </source>
    </evidence>
    <sequence length="581" mass="63668" checksum="107AB96626C05C82" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZV7</accession>
    <name>A0A6I8VZV7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Semaphorin-1A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Semaphorin-I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC4816208</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236630.1">
        <property type="nucleotide sequence ID" value="XM_033380739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZV7"/>
    <dbReference type="FunCoup" id="A0A6I8VZV7">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VZV7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VZV7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11237">
        <property type="entry name" value="Sema_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000016">
        <property type="entry name" value="Putative Semaphorin-6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000346">
        <property type="entry name" value="Sema-1a, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042068">
        <property type="entry name" value="SEM1A_sema_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF127">
        <property type="entry name" value="SEMAPHORIN-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="631"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="16">
        <location>
            <begin position="42"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="711"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="770"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="784"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="803"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="822"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033236630.1"/>
        </source>
    </evidence>
    <sequence length="878" mass="98792" checksum="3996DB882082A5CB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0W8</accession>
    <name>A0A6I8W0W8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein C1orf50 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184263</name>
        <name evidence="2" type="synonym">LOC4817150</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3">MV-25-SWS-2005</strain>
            <tissue evidence="2 3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356386.3">
        <property type="nucleotide sequence ID" value="XM_001356350.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236913.1">
        <property type="nucleotide sequence ID" value="XM_033381022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0W8"/>
    <dbReference type="KEGG" id="dpo:117184263"/>
    <dbReference type="KEGG" id="dpo:4817150"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076501">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W0W8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019534">
        <property type="entry name" value="DUF2452"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14553:SF1">
        <property type="entry name" value="SIMILAR TO CHROMOSOME 1 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14553">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN C1ORF50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10504">
        <property type="entry name" value="DUF2452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356386.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236913.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="21665" checksum="8E7859F5617F7B12" modified="2020-08-12" version="1">MKRATTMDQLTYTTAVKKAQLVERNPQSLLDPLRVSMHDQEDIIALAQQIQNADKQLKHTTCHKLGVIMDQIKMLQAQAMEILKESNVNRDLHNAACNFTKKPGQIYHLYQRASGQTYFSMLSPEEWNHSVDQTYKGSYRLEFDLSWTPLDKIKQQDERLKWAEECMEKALDSGRSAMAIDFNINNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W2L3</accession>
    <name>A0A6I8W2L3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">app</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="RefSeq" id="XP_033237611.1">
        <property type="nucleotide sequence ID" value="XM_033381720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W2L3"/>
    <dbReference type="InParanoid" id="A0A6I8W2L3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="145"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="373"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="564"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="566"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="582"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="611"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033237611.1"/>
        </source>
    </evidence>
    <sequence length="786" mass="85156" checksum="976BB6B44ABB0A9F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4H1</accession>
    <name>A0A6I8W4H1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein rtv isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rtv</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033238222.1">
        <property type="nucleotide sequence ID" value="XM_033382331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4H1"/>
    <dbReference type="FunCoup" id="A0A6I8W4H1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W4H1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032222">
        <property type="term" value="P:regulation of synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23589">
        <property type="entry name" value="TFP_LU_ECD_Rtv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031424">
        <property type="entry name" value="QVR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050975">
        <property type="entry name" value="Sleep_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562">
        <property type="entry name" value="ATILLA, ISOFORM B-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562:SF22">
        <property type="entry name" value="PROTEIN QUIVER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17064">
        <property type="entry name" value="QVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026346028" evidence="9">
        <location>
            <begin position="26"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238222.1"/>
        </source>
    </evidence>
    <sequence length="174" mass="19459" checksum="08A6FD8FA669E6C9" modified="2020-08-12" version="1">MKYISICLAVIFLFSCSFLFGKIHGESSGQVCALFKSFVICFHLCLLRRCYQCRSRGDLGSCKDPFNFNATDVDHEPGLAAIPCASGWCGKVIEGGGTYAIDDYDLAIQRMCVQRGPDDNMDRCADTIYNYKKVYMCFCQGDLCNKGHSWTQPSGRLVLMFPLLAVLLPFSRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4T6</accession>
    <name>A0A6I8W4T6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12 13">Probable G-protein coupled receptor B0563.6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">LOC6901015</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12 13">MV-25-SWS-2005</strain>
            <tissue evidence="11 12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238373.1">
        <property type="nucleotide sequence ID" value="XM_033382482.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033382483.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238375.1">
        <property type="nucleotide sequence ID" value="XM_033382484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4T6"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243732">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14978">
        <property type="entry name" value="7tmA_FMRFamide_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053093">
        <property type="entry name" value="GPCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47760">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR B0563.6-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47760:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
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            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="79"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="418"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033238374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033238375.1"/>
        </source>
    </evidence>
    <sequence length="457" mass="51766" checksum="01BCC6FD52CB255F" modified="2020-08-12" version="1">MITRLYHTEEDPAYCSFIWGSNLSSSTDVLDAAANATPVFGTDLRDDIYRDVEDPRTQTLREYCYGILLPIICAMGIIGNVLNLIVLTRRNMRGTAYIYMRAYSAAALLAILFAIPFGIRMQVHKDRGQWEEFGPAFYTAHLELYLGNGCLGVGVMMLLVLTLERYVSVCHPGVTRPVMGRPGLVVFLTSLVTIIAYLPSIFRGELIKCILSSPDVYVYLRRDNTIYQQTMFYKVYKIMLEMVFKLIPTVVIGGLNLQIMIVYRQTCERRRQMVLTRANSNFHKDDDPRKFAEERRLFLLLGSTSILFLVCVSPMAILHMTIASEVYPSFPFQVFRASANLLELINYSLTFYIYCLFSEDFRNTLFRTFKWPWLKGQLCHQVDLEVSASPPATAATAAATPQAGNSSNYTTTVTIPKCMPRLPNPTRHPHQLARPDRKGHANGALREGVQASPFQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6H9</accession>
    <name>A0A6I8W6H9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Proton channel OtopLc isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">OtopLa</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238960.1">
        <property type="nucleotide sequence ID" value="XM_033383069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6H9"/>
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        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815622"/>
    <dbReference type="InParanoid" id="A0A6I8W6H9"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074679">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF62">
        <property type="entry name" value="OTOPETRIN-LIKE A, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="713"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="781"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="807"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="853"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="884"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="926"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="214"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="524"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033238960.1"/>
        </source>
    </evidence>
    <sequence length="1002" mass="110038" checksum="AAE668F60A664260" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6Y2</accession>
    <name>A0A6I8W6Y2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117184651</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239125.1">
        <property type="nucleotide sequence ID" value="XM_033383234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6Y2"/>
    <dbReference type="KEGG" id="dpo:117184651"/>
    <dbReference type="InParanoid" id="A0A6I8W6Y2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239125.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="40790" checksum="3A07314010DD7D89" modified="2020-08-12" version="1">MAEETMANIDKEKIVEWLLEKEIIFPASATLRQLRKLAISGGMDEVSESLVNKSDIESEEKVSENEQIDDMKEEELLDAAIRVAEKKKKLAALMVMDNHVTDDIRMVKQLIAPFSVSENDEASEWVLNFERICGGVNESSNFKLRCVRMLMKAGTEADLFMRVDKSKTYDEFKGNFLKTFGRSNSTADVVLLLKETFFNPEKNSVMGYILRMEEIAMRADIEEKLTVQFIIDGFRDRSSSIALLYSASTIGKLKELARQYEALRKSTQTNFRRTGMTASGNDRSQARCYNCSAHGHYASSCPAPKREKGSCFQCGSMQHQVKDCQQKPMSAQRSVGTASNPPMDDREENNIFVPIVNQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WA53</accession>
    <name>A0A6I8WA53_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813586</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240268.1">
        <property type="nucleotide sequence ID" value="XM_033384377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WA53"/>
    <dbReference type="KEGG" id="dpo:4813586"/>
    <dbReference type="InParanoid" id="A0A6I8WA53"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240268.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="16346" checksum="9CC30EB19F8859D8" modified="2020-08-12" version="1">MAENINKSNIIHCKDTEQLNETVNSNSDETSSSGDIENSRYGKLRRRSVRSFSYHYQALPMRVVGDQSASISGYSSSARDITLGPAHIIEDSSESEFHISDTSNEEPANKSIKFVGRPLGKTDLDFIKKQYKRKKRPKKKIYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAD1</accession>
    <name>A0A6I8WAD1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117185022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240361.1">
        <property type="nucleotide sequence ID" value="XM_033384470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WAD1"/>
    <dbReference type="KEGG" id="dpo:117185022"/>
    <dbReference type="InParanoid" id="A0A6I8WAD1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="1">
        <location>
            <begin position="50"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240361.1"/>
        </source>
    </evidence>
    <sequence length="169" mass="19706" checksum="FE6F32EB1BDE6C22" modified="2020-08-12" version="1">MNKLDQKLDILINKLDRNTAEIVAHRSEIGSIKAKVCGEIRKPKVVLPCQPLKTIEELDHFEHNLQEESFFKNVIAELMMSGEKAFDKWIRSSWRTIVSDEVARQCSWRGTEEKKCIRGLRVTLAIRTGFKERFSLEDADFDRVTQKLLQYAQDRVSKEKKQINLNSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WE72</accession>
    <name>A0A6I8WE72_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein pasi2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pasi2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241688.1">
        <property type="nucleotide sequence ID" value="XM_033385797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WE72"/>
    <dbReference type="FunCoup" id="A0A6I8WE72">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800606"/>
    <dbReference type="InParanoid" id="A0A6I8WE72"/>
    <dbReference type="OMA" id="MIFAYNI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080827">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060857">
        <property type="term" value="P:establishment of glial blood-brain barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035159">
        <property type="term" value="P:regulation of tube length, open tracheal system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019991">
        <property type="term" value="P:septate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36694:SF4">
        <property type="entry name" value="LD42595P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36694">
        <property type="entry name" value="PASIFLORA 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241688.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="29254" checksum="828FC2FCDBFBF7CA" modified="2020-08-12" version="1">MNYGRKTPSTYRSNPSVYSHATGRSSTNLHSKMSRSTRSVRIPWYQRPLLKNNQYIDIQKGAMLIGLFAIFLSLFTIASSVFDIYCYAMAAPGSTHYGYYIISYEFVYVGNKHVRNMLIVFALFSLIMALINFVTSVLLCVALRKEYERKVMPWLWTFAVFTVWRALALIFFAIVNDLYFAYNVIMVMLWSIFCLLSIYGWAVVYSLFLELVDLTKLEDLAHLRMGTMASLHASTANSLAGSRPTTPHSTVSTMPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>B5DIG6</accession>
    <accession>A0A6I8UXJ0</accession>
    <name>B5DIG6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Proline-rich extensin-like protein EPR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TwdlE</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132706.1">
        <property type="nucleotide sequence ID" value="XM_002132670.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DIG6"/>
    <dbReference type="FunCoup" id="B5DIG6">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DIG6"/>
    <dbReference type="GeneID" id="6902981"/>
    <dbReference type="KEGG" id="dpo:6902981"/>
    <dbReference type="eggNOG" id="ENOG502S006">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098085_0_0_1"/>
    <dbReference type="InParanoid" id="B5DIG6"/>
    <dbReference type="OMA" id="IVHKHVY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247355">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043702470" evidence="2">
        <location>
            <begin position="20"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="3">
        <location>
            <begin position="59"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002132706.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="21116" checksum="8202AE97FD89DFC0" modified="2008-10-14" version="1">MQSSCVLVIVLGLAALVVARPEPPRDSYSAPPSSSYQPSGPSGGYGAPAPQYGPPQQPPVVHKHVYVHVPPPEPEYQAPRKPLYVPPPQKHYKIVFIKAPSAPAPTAPIIPQYPQNEEKTLVYVLVKKPEEQPEIIIPTPAPTQPSKPEVYFIRYKTQKEETGPYPNSVAPPAPEYGAPAGPPAPSAPSSSYGAPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKL8</accession>
    <accession>A0A6I8UYM9</accession>
    <name>B5DKL8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6901905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133440.1">
        <property type="nucleotide sequence ID" value="XM_002133404.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKL8"/>
    <dbReference type="FunCoup" id="B5DKL8">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="GeneID" id="6901905"/>
    <dbReference type="KEGG" id="dpo:6901905"/>
    <dbReference type="eggNOG" id="ENOG502T84U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076195_0_0_1"/>
    <dbReference type="InParanoid" id="B5DKL8"/>
    <dbReference type="OMA" id="AWLLQCP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244218">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DKL8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133440.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="35036" checksum="C5122D38C87EFCBB" modified="2008-10-14" version="1">MGDDQKINARKHVKQIFQDFLEENDCEALKPNCANNSCCGRGLQEFDAQTHEAWLLQCPKSLDPSALEGRRIKMPGRRFVGGLQVRCVEYSTPSSESIGYTNVKGKNRVRKFPLSGVVVLSKRLNVAQPGANEEANSFPTTATPPSFVMPVRHPFFGRDYKKRIKVTKAISRHLIEANKRSLETSALLRDKANYYKIRKQLQGTTQTLEEKEQDVRQSVLTGVTPDFMTKGHGASYNLGDLTSGDKKVRTKKKHNTQKEKISQDQLLSAEKPKKRTANGKVKTNGDVKHDQQDGADEAPKKKRKKLKTNGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E95</accession>
    <accession>A0A6I8UB18</accession>
    <name>Q29E95_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein hoka</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hoka</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352668.2">
        <property type="nucleotide sequence ID" value="XM_001352632.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E95"/>
    <dbReference type="FunCoup" id="Q29E95">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E95"/>
    <dbReference type="GeneID" id="4811993"/>
    <dbReference type="KEGG" id="dpo:4811993"/>
    <dbReference type="eggNOG" id="ENOG502SCEV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139367_0_0_1"/>
    <dbReference type="InParanoid" id="Q29E95"/>
    <dbReference type="OMA" id="HETANVT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072517">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044246979" evidence="3">
        <location>
            <begin position="28"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001352668.2"/>
        </source>
    </evidence>
    <sequence length="126" mass="13661" checksum="CD6C8D7DA834EA7B" modified="2008-10-14" version="2">MKLAKKCSTYLVICLVLLACCLEETEATRRVNRGRRTLTRRYFNGLAIPGWAMIICVALGELIVGGALYFIMKKLILDKEPDETATSYTPAATTYTPAATTYTPAQTHEPAAATPSPPPTHATTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FS0</accession>
    <accession>A0A6I8UF31</accession>
    <name>Q29FS0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sorting nexin-27 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Snx27</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354456.2">
        <property type="nucleotide sequence ID" value="XM_001354420.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FS0"/>
    <dbReference type="SMR" id="Q29FS0"/>
    <dbReference type="FunCoup" id="Q29FS0">
        <property type="interactions" value="1515"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FS0"/>
    <dbReference type="GeneID" id="4814401"/>
    <dbReference type="KEGG" id="dpo:4814401"/>
    <dbReference type="eggNOG" id="KOG3784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028138_0_0_1"/>
    <dbReference type="InParanoid" id="Q29FS0"/>
    <dbReference type="OMA" id="PNEFPHN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077133">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13338">
        <property type="entry name" value="FERM-like_C_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01777">
        <property type="entry name" value="FERM_F1_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23070">
        <property type="entry name" value="PDZ_SNX27-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06886">
        <property type="entry name" value="PX_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000210">
        <property type="entry name" value="Putative Sorting nexin-27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.60:FF:000003">
        <property type="entry name" value="Sorting nexin-27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000061">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000003">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037827">
        <property type="entry name" value="SNX27_FERM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037833">
        <property type="entry name" value="SNX27_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037835">
        <property type="entry name" value="SNX27_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF19">
        <property type="entry name" value="SORTING NEXIN-27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001354456.2"/>
        </source>
    </evidence>
    <sequence length="514" mass="57906" checksum="500D581D60D005AF" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FV5</accession>
    <name>NNRE_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)H-hydrate epimerase</fullName>
            <ecNumber>5.1.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NAD(P)HX epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GA15549</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <dbReference type="EC" id="5.1.99.6"/>
    <dbReference type="EMBL" id="CH379065">
        <property type="protein sequence ID" value="EAL31474.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FV5"/>
    <dbReference type="SMR" id="Q29FV5"/>
    <dbReference type="FunCoup" id="Q29FV5">
        <property type="interactions" value="1400"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FV5"/>
    <dbReference type="eggNOG" id="KOG2585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_3_0_1"/>
    <dbReference type="InParanoid" id="Q29FV5"/>
    <dbReference type="OMA" id="CHHMEIP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032976">
        <property type="entry name" value="YJEFN_prot_NAXE-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232:SF10">
        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000379430" description="NAD(P)H-hydrate epimerase">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="127"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="2">
        <source>
            <dbReference type="EMBL" id="EAL31474.2"/>
        </source>
    </evidence>
    <sequence length="226" mass="25188" checksum="B0A9C829E5413811" modified="2008-10-14" version="2">MVKYLSQSEAIELDLDLFEMFHVGQLMELAGLSCADAVAECFPAQTHPRVLVVCGPGNNGGDGLVCARHLSLMGYQPTVYYPKPTLMSLYENLTNQCHHMEIPSVKKCPSVSDAEEDYDLILDALFGFGFKPPVREDFVPLVKMMQETKVPIASVDIPSGWDVEKGKQSECDFEPKLLISLTAPKLCAEHFKGEHHYLGGRFVPPALQRKYQLNLPDYGSKLVVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q29G51</accession>
    <accession>A0A6I8UGN4</accession>
    <name>Q29G51_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="6 9">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Ahcy</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="8 9">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="6 9"/>
    <dbReference type="RefSeq" id="XP_001355202.1">
        <property type="nucleotide sequence ID" value="XM_001355166.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29G51"/>
    <dbReference type="SMR" id="Q29G51"/>
    <dbReference type="FunCoup" id="Q29G51">
        <property type="interactions" value="1348"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29G51"/>
    <dbReference type="GeneID" id="4814720"/>
    <dbReference type="KEGG" id="dpo:4814720"/>
    <dbReference type="eggNOG" id="KOG1370">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_1"/>
    <dbReference type="InParanoid" id="Q29G51"/>
    <dbReference type="OMA" id="YIGVTVE"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071176">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenosylhomocysteinase-like"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
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        <property type="entry name" value="Adenosylhomocysteinase-like"/>
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        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="11">
        <location>
            <begin position="191"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
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    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="186"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="190"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="222"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="299"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034527"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001355202.1"/>
        </source>
    </evidence>
    <sequence length="432" mass="47389" checksum="785FA7378CC19A22" modified="2006-04-04" version="1">MSKPAYKVADISLAEWGRKAIIIAENEMPGLMACRKKYGPSKILKGARITGCLHMTVQTAVLIETLVELGAEVQWSSCNIFSTQDNAAAAIAATGVPVYAWKGETDEEYMWCIEQTLVFPDGKPLNMILDDGGDLTNLVHEKFPQYLKDIKGLSEETTTGVHNLYKMFKDGRLGIPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVCCVAGYGDVGKGCAQALKGFGGRVIVTEIDPINALQAAMEGYEVTTMEEASKEASIFVTTTGCRDIITSVHLLNMPDDAIVCNIGHFDIEIDVDWLNANAKEKVNVKPQVDRYTMPNGRHIILLAEGRLVNLGCAHGHPSFVMSNSFTNQVLAQIELWTKSAQYKVGVHVLPKVLDEEVASLHLEKLGVKLTKLTEKQSSYLGVPKTGPFKPDHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29ID8</accession>
    <accession>A0A6I8WE18</accession>
    <name>Q29ID8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4815899</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240804.1">
        <property type="nucleotide sequence ID" value="XM_033384913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29ID8"/>
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        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815899"/>
    <dbReference type="eggNOG" id="KOG3659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006855_7_0_1"/>
    <dbReference type="InParanoid" id="Q29ID8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070626">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29ID8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10332">
        <property type="entry name" value="SLC6sbd-B0AT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
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    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF182">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="5 9" id="KW-0769">Symport</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="474"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="515"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="546"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="590"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="633"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="489"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033240804.1"/>
        </source>
    </evidence>
    <sequence length="724" mass="80992" checksum="DC44A3EECD79DE57" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q29J95</accession>
    <accession>A0A6I8VGX3</accession>
    <name>Q29J95_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">GPI-anchor transamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">PIG-K</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355349.1">
        <property type="nucleotide sequence ID" value="XM_001355313.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041044.1">
        <property type="nucleotide sequence ID" value="XM_015185558.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29J95"/>
    <dbReference type="SMR" id="Q29J95"/>
    <dbReference type="STRING" id="46245.Q29J95"/>
    <dbReference type="MEROPS" id="C13.005"/>
    <dbReference type="GeneID" id="4815521"/>
    <dbReference type="KEGG" id="dpo:4815521"/>
    <dbReference type="eggNOG" id="KOG1349">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044656_1_0_1"/>
    <dbReference type="OMA" id="VMESQFP"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078170">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29J95">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000002">
        <property type="entry name" value="GPI-anchor transamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500138">
        <property type="entry name" value="GPI8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019663">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014308605" evidence="6">
        <location>
            <begin position="22"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR019663-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001355349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015041044.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="40187" checksum="01F597F4854F40DA" modified="2006-04-04" version="1">MNLKLVSIVYIFCICGISCTASNEDTSVLPEGFVDATQRSTHTNNWAVLVDASRFWFNYRHVANVLSIYRSVKRLGIPDSQIILMIADDMACNARNPRPGQVYNNANQHINVYGDDVEVDYRGYEVTVENFVRLLTGRTQNGTARSKKLLSDAGSNVLIYLTGHGGDGFLKFQDSEEITSQELADGIQQMWEKKRYNELFFMVDTCQAASLYEKFSSPNVLAVASSLVGQDSLSHHVDPSIGVYMIDRYTYYALEFLEKVQPFSTRTIGDFLQVCPKRVCISTVGVRKDLYRRDPHKVPITDFFGAIRPTRVSTDRINVTLANESDFIADPDEISANKRPFKIIMEPQFPSEFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LI6</accession>
    <accession>A0A6I8VVS7</accession>
    <name>Q29LI6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nascent polypeptide-associated complex subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gNacalpha</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235187.1">
        <property type="nucleotide sequence ID" value="XM_033379296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LI6"/>
    <dbReference type="FunCoup" id="Q29LI6">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LI6"/>
    <dbReference type="KEGG" id="dpo:4817590"/>
    <dbReference type="eggNOG" id="KOG2239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057806_1_1_1"/>
    <dbReference type="InParanoid" id="Q29LI6"/>
    <dbReference type="OMA" id="EDITQMA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078176">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14358">
        <property type="entry name" value="UBA_NAC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033235187.1"/>
        </source>
    </evidence>
    <sequence length="277" mass="30268" checksum="77D73F705B86C5A8" modified="2008-10-14" version="2">MGKKQKNQKAAATAAATASATAAATAAINVGEAAFKAATKDMWLDKALTFGRDEEAPSTSKKSKNPDYNEAMQQLMEMTKKYPQLGDHACTALSDDEVDSDDDVTDAVGSLDPQAGAMPLGAHRQSRGEKKARRLLSKLDLKPVENVSRVTMRKNKNILLYIDQPDVFKVGDSYLCFGDVKVEDITQMATAQAAERFRENATAADQSEREALAWAKANENDQEEPEFDEELLMELDDKDIDLVQMQATCSRTKAIKALLDNENDVVNAIMALTLASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LJ8</accession>
    <accession>A0A6I8UKI5</accession>
    <name>Q29LJ8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">morgue</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001356981.1">
        <property type="nucleotide sequence ID" value="XM_001356945.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LJ8"/>
    <dbReference type="SMR" id="Q29LJ8"/>
    <dbReference type="FunCoup" id="Q29LJ8">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LJ8"/>
    <dbReference type="GeneID" id="4817576"/>
    <dbReference type="KEGG" id="dpo:4817576"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045184_0_0_1"/>
    <dbReference type="InParanoid" id="Q29LJ8"/>
    <dbReference type="OMA" id="WMASEVC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073763">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LJ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23826">
        <property type="entry name" value="UEV_Morgue-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000060">
        <property type="entry name" value="Ubiquitin conjugating enzyme (UbcB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356981.1"/>
        </source>
    </evidence>
    <sequence length="488" mass="55574" checksum="3414AD8240C040E5" modified="2006-04-04" version="1">MTSCVSAQQQKDQEQLLDELNASDDEMDDAEDADKAKEQLTEPTFSFSNSHTCWVCNGYYGPNFGEPLCGACHAFLYNAQLAEEMLTELSDDEDSGNDEPPFKDKQEDEETENDVDIMGFEDLEDAEPAAQPHQPQPPLPVNNEADAVVQQLELPQQAQDLVALQPPARRESPERDFDNERAVNPFLLPVKRPRPMAPLGLPHFMNELADGRLRSHEYNANGAGSSNAADNKSIINRIPVEILLKIFAYLDDMSLWMCSEVCKQWHDIIGKNTAQSMWKAYIKQRWPLFESLSDSPDWHKLYGALMSSCFCRTCLIEMGGRGQSPQDDEQDNPIRNIFLRGEANLVNSYESEGISAIPLDRQNNYWQATILGPPGSPYEGGKFFLFIYFPDRYPMAPPTVRFLTKILHPNVSRHGDVGIDIFQQQNWSLALNVAKVLLSVQSLLTDPYTEVCMEPEVGYIYEHERERFEQLVRSWTWKYAMFELISPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LT5</accession>
    <accession>A0A6I8UKF4</accession>
    <name>Q29LT5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protein NEDD8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Neddylin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Nedd8</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356894.2">
        <property type="nucleotide sequence ID" value="XM_001356858.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LT5"/>
    <dbReference type="SMR" id="Q29LT5"/>
    <dbReference type="FunCoup" id="Q29LT5">
        <property type="interactions" value="2965"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LT5"/>
    <dbReference type="GeneID" id="4817338"/>
    <dbReference type="KEGG" id="dpo:4817338"/>
    <dbReference type="eggNOG" id="KOG0005">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_6_4_1"/>
    <dbReference type="InParanoid" id="Q29LT5"/>
    <dbReference type="OMA" id="YAGKQMA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070545">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LT5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd01806">
        <property type="entry name" value="Ubl_NEDD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000023">
        <property type="entry name" value="NEDD8 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038738">
        <property type="entry name" value="Nedd8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001356894.2"/>
        </source>
    </evidence>
    <sequence length="80" mass="8949" checksum="2736CAA822DF42D6" modified="2008-10-14" version="2">MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGHSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NI7</accession>
    <accession>A0A6I8UL35</accession>
    <name>Q29NI7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Small nuclear ribonucleoprotein-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SmB</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357165.3">
        <property type="nucleotide sequence ID" value="XM_001357129.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NI7"/>
    <dbReference type="FunCoup" id="Q29NI7">
        <property type="interactions" value="1530"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NI7"/>
    <dbReference type="GeneID" id="4817773"/>
    <dbReference type="KEGG" id="dpo:4817773"/>
    <dbReference type="eggNOG" id="KOG3168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_1_0_1"/>
    <dbReference type="InParanoid" id="Q29NI7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078820">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01717">
        <property type="entry name" value="Sm_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000004">
        <property type="entry name" value="Small nuclear ribonucleoprotein-associated proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017131">
        <property type="entry name" value="snRNP-assoc_SmB/SmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037187">
        <property type="entry name" value="snRNP_SmB/SmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 11 15" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="169"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001357165.3"/>
        </source>
    </evidence>
    <sequence length="199" mass="20911" checksum="E54F3C93C9B98AFA" modified="2016-01-20" version="3">MTIGKNNKMIQHLNYRVRIVLQDSRTFIGTFKAFDKHMNLILGDCEEFRKIRSKNSKVPEREEKRVLGFVLLRGENIVSLTVEGPPPPEEGLPRVPIPGAASGPGIGRIAGRGVPLNMSAVPAGLQGPVRGIGGPAQQHMAPMGRGVPRAPMMGAPPPGLIPGGIPSLPGNLGRGAPPPMRGPPPSIMRGAPPAGRGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0P7</accession>
    <accession>A0A6I8VKF0</accession>
    <name>Q2M0P7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">General transcription factor IIH subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Ssl1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GTF2H2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015042798.1">
        <property type="nucleotide sequence ID" value="XM_015187312.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0P7"/>
    <dbReference type="SMR" id="Q2M0P7"/>
    <dbReference type="FunCoup" id="Q2M0P7">
        <property type="interactions" value="1405"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0P7"/>
    <dbReference type="GeneID" id="4812910"/>
    <dbReference type="KEGG" id="dpo:4812910"/>
    <dbReference type="eggNOG" id="KOG2807">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028556_1_1_1"/>
    <dbReference type="InParanoid" id="Q2M0P7"/>
    <dbReference type="OMA" id="INWVEVP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070823">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0P7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="12">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015042798.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="49530" checksum="8E1CC0E6A39AE75D" modified="2008-10-14" version="2">MADDDRDDQKEYRWETGYEKTWEAIKDDEDGLLDGAIADIIQKAKRQRQAQKTKQNRLGMMRHLFIILDCSESMSVPDLKPTRLRCTIKLLDQFIEEFFDQNPISQMGIIALKAKRAEKITELTGTSRVHLKALEGLANVPLTSEPSLQNGLDLALKTLKVVPSHASREIVIVMGSLTTCDPVDINLTIDELKKEGIRCSVISLSAEIHIARYLTQQTRGTFGAVLDDAHFRDQLMSQVDPPPAAKTQHNSLIRMGFPHTKNEVEGKDAPLSMCMCHIENLEEPSELCTTGHHCPQCNSKYCELPVECQSCGLTLVSAPHLARSYHHLFPVPNFEEIPFDSLPKASSDRGGTGTGTTECYACMKLFVPVADKTVFKCGFCKQFFCLDCDIFIHDTLHACVGCNTIPGVDGLVYQQSSKSVSDSHHQAQAHDLHPGTSKQRGQFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NYE7</accession>
    <accession>A0A6I8V8N1</accession>
    <name>A0A0R3NYE7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein drumstick</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">drm</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17">MV-25-SWS-2005</strain>
            <tissue evidence="16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015036107.1">
        <property type="nucleotide sequence ID" value="XM_015180621.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036108.1">
        <property type="nucleotide sequence ID" value="XM_015180622.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NYE7"/>
    <dbReference type="GeneID" id="4817188"/>
    <dbReference type="KEGG" id="dpo:4817188"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070069">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NYE7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007366">
        <property type="term" value="P:periodic partitioning by pair rule gene"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001793">
        <property type="entry name" value="Blast:Protein drumstick"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000712">
        <property type="entry name" value="Drumstick, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF0">
        <property type="entry name" value="PROTEIN BOWEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0562">Pair-rule protein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015036107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015036108.1"/>
        </source>
    </evidence>
    <sequence length="88" mass="10772" checksum="ACDDA0CEC3F4641E" modified="2016-01-20" version="1">MFAVMRIDNDDCRSDFRRKMRPKCEFICKYCQRRFTKPYNLMIHERTHKSPEITYSCEVCGKYFKQRDNLRQHRNLHKDTCGRGGYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P5P8</accession>
    <accession>A0A6I8VKZ0</accession>
    <name>A0A0R3P5P8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6899900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015043528.1">
        <property type="nucleotide sequence ID" value="XM_015188042.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P5P8"/>
    <dbReference type="GeneID" id="6899900"/>
    <dbReference type="KEGG" id="dpo:6899900"/>
    <dbReference type="InParanoid" id="A0A0R3P5P8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249799">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015043528.1"/>
        </source>
    </evidence>
    <sequence length="182" mass="21075" checksum="CD8CD5B9C28305AA" modified="2016-01-20" version="1">MSTDMSWDIFCCICSSCFIACIVRQSNAGQAQCESLVEYENTPFRLSCEAKYSRSLKLHYRDRRIRTDTPYRVWVRGDPRRHIELLITLHKSPLWDCGSVRCSPNWLHCHCQHSYGIGIAIRPDSIFCYKFNFSYVGELQSECGMKPLRVDFVAIHYGVWYRDAGRISSSGAGRLKWALWSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB01</accession>
    <name>A0A6I8UB01_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mucin-5AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Tie</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352645.4">
        <property type="nucleotide sequence ID" value="XM_001352609.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UB01"/>
    <dbReference type="FunCoup" id="A0A6I8UB01">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812491"/>
    <dbReference type="InParanoid" id="A0A6I8UB01"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080408">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000689">
        <property type="entry name" value="Tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000577">
        <property type="entry name" value="Tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF481">
        <property type="entry name" value="TIE-LIKE RECEPTOR TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026173010" evidence="6">
        <location>
            <begin position="28"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="708"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="832"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="221"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="357"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="480"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="640"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="759"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1044"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="358"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="491"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="522"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1060"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="867"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001352645.4"/>
        </source>
    </evidence>
    <sequence length="1213" mass="131780" checksum="3B54BAB807E45E92" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBF3</accession>
    <name>A0A6I8UBF3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="4">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">eIF5B</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts through its C-terminal domain (CTD) with the CTD of eIF1A (EIF1AX) or with the CTD of EIF5 (mutually exclusive) through a common binding site. Interacts with eIF1A (EIF1AX) from the location of the start codon by the 43S complex until the formation of the 80S complex. Interacts with ANXA5 in a calcium and phospholipid-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001352861.4">
        <property type="nucleotide sequence ID" value="XM_001352825.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBF3"/>
    <dbReference type="FunCoup" id="A0A6I8UBF3">
        <property type="interactions" value="2197"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812430"/>
    <dbReference type="InParanoid" id="A0A6I8UBF3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBF3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16266">
        <property type="entry name" value="IF2_aeIF5B_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000026">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
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            <taxon>Neoptera</taxon>
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            <taxon>Brachycera</taxon>
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            <taxon>Drosophila</taxon>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
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    </reference>
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        <property type="nucleotide sequence ID" value="XM_015188461.2"/>
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        <property type="nucleotide sequence ID" value="XM_015188462.2"/>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <text evidence="2">Belongs to the dynactin 150 kDa subunit family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001353608.2"/>
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    <sequence length="1263" mass="141250" checksum="09B8FD9DB4D9F122" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEE3</accession>
    <name>A0A6I8UEE3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-glucuronic acid decarboxylase 1</fullName>
            <ecNumber evidence="5">4.1.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">UDP-glucuronate decarboxylase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">Uxs</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>UDP-alpha-D-glucuronate + H(+) = UDP-alpha-D-xylose + CO2</text>
            <dbReference type="Rhea" id="RHEA:23916"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="EC" id="4.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:23917"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001354211.3">
        <property type="nucleotide sequence ID" value="XM_001354175.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEE3"/>
    <dbReference type="FunCoup" id="A0A6I8UEE3">
        <property type="interactions" value="1336"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814160"/>
    <dbReference type="InParanoid" id="A0A6I8UEE3"/>
    <dbReference type="UniPathway" id="UPA00796">
        <property type="reaction ID" value="UER00771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UEE3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033320">
        <property type="term" value="P:UDP-D-xylose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05230">
        <property type="entry name" value="UGD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000065">
        <property type="entry name" value="UDP-glucuronic acid decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF6">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="19">
        <location>
            <begin position="127"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001354211.3"/>
        </source>
    </evidence>
    <sequence length="454" mass="51562" checksum="E200A5C393C17E00" modified="2020-08-12" version="1">MMTATKKRIKIVAAASFSLLLVVYLYRAISASTVGMPGGVGVVGRLSGPIEEEQQGQWPPTEDAIQRSLQNAYDAHNSLIKEQRAELQRTKEQLAQLEEQIRSLQTSTPRKYPKVKYLNYKNRKRILITGGAGFVGSHLVDDLMIQGHEVIVVDNFFTGRKRNVAHWLGHENFELIHHDIVNPLFIEIDEIYHLASPASPPHYMYNPVKTIKTNTMGTINVLGLAKRVMAKVLIASTSEVYGDPTVHPQPETYWGHVNPIGPRACYDEGKRVSETLSYAYAKQEKVQVRVARIFNTYGPRMHMNDGRVVSNFILQALRNETITVYGNGRQTRSFQYVSDLVDGMIALMASNYTQPVNLGNPVEQSIEEFAQIIKQLVGGPSPIKQTKAVEDDPQRRKPDITRARHYLKWEPKVPLERGLRQTISYFRNELARSDRFQESSNKYFDSPDLQRSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF57</accession>
    <name>A0A6I8UF57_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL16m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L16, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mRpL16</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354517.2">
        <property type="nucleotide sequence ID" value="XM_001354481.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UF57"/>
    <dbReference type="FunCoup" id="A0A6I8UF57">
        <property type="interactions" value="601"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815339"/>
    <dbReference type="InParanoid" id="A0A6I8UF57"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UF57">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000005">
        <property type="entry name" value="39S ribosomal protein L16, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_uL16_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220:SF13">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001354517.2"/>
        </source>
    </evidence>
    <sequence length="243" mass="27996" checksum="49834E3336C9F407" modified="2020-08-12" version="1">MWSVKTVTQLFKNSLSTGKFAAINTAGLKYFAPPIKYQNVEQPERPKLRIMERMPQLPPNLRPPKMQKRLRYMRGPEPVHNSLLHKQYAIVATGGGRLRWGHYEMMRLTIGRKMNVQTMFATWRVPAPWQPITKKGQGQRMGGGKGAIDHYVTPIRAGRVIVEIAGKCEFVEVKGFLQQVANQLPFQATVVSQAMLDERLAEEEQYARENQNPFTMKYVIQNNLNGCHRWLSPVDHKWFGKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFT8</accession>
    <name>A0A6I8UFT8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 137</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4814741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354799.3">
        <property type="nucleotide sequence ID" value="XM_001354763.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFT8"/>
    <dbReference type="FunCoup" id="A0A6I8UFT8">
        <property type="interactions" value="1531"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814741"/>
    <dbReference type="InParanoid" id="A0A6I8UFT8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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</entry>
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    <accession>A0A6I8UGS7</accession>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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                <consortium name="RefSeq"/>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <begin position="1"/>
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            <begin position="497"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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            <dbReference type="RefSeq" id="XP_001355465.3"/>
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    <sequence length="632" mass="70914" checksum="96305AD2D1B7C8A0" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8UHH5</accession>
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        <recommendedName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glyco_transf_92"/>
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    </dbReference>
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            <begin position="6"/>
            <end position="25"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU366017"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355471.3"/>
        </source>
    </evidence>
    <sequence length="561" mass="65512" checksum="E8FEDB13E0852C92" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIL0</accession>
    <name>A0A6I8UIL0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein insensitive</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">insv</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355982.3">
        <property type="nucleotide sequence ID" value="XM_001355946.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIL0"/>
    <dbReference type="FunCoup" id="A0A6I8UIL0">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816341"/>
    <dbReference type="InParanoid" id="A0A6I8UIL0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIL0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2590:FF:000005">
        <property type="entry name" value="Early boundary activity protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037496">
        <property type="entry name" value="BEND6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346:SF1">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01025">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BEN" evidence="8">
        <location>
            <begin position="259"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="219"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355982.3"/>
        </source>
    </evidence>
    <sequence length="376" mass="41897" checksum="19D83E0EDF3C55C1" modified="2020-08-12" version="1">MDSNVYKRVVSANGSHFVSVHDEKKNRGREEPLMLNAWSQTNSDDFEVLRSVNIDSNMDAENRVLLEKVRYLENKLKEHTDLLSQIHATSARMQQSLGVADAASPTPPVYPSHQILTPPSSVICAPVQGSQQQRTEVLDYKIISASDDADAIEIRLAAESLNSLSSSSDSDRLEMCIGTDSQNQTQPSPQILNRDIKRELSRQQETAPLQFIKRRKLQEIQVQETPSPKTKPKLPLKLKPRTSDPSNGQSNDNVMISLGPNNTCVPASVFENINWTACSLATRKLLVTIFDRETLATHSMTGKPSPAFRDQPKPLKQMLDPAKIQDIIFAVTHKCNASEKEVRNAITTKCADENKMLKIQNGKRRTSGVNGKENMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIV0</accession>
    <name>A0A6I8UIV0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate synthase</fullName>
            <ecNumber evidence="3">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Ts</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001356150.2">
        <property type="nucleotide sequence ID" value="XM_001356114.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIV0"/>
    <dbReference type="FunCoup" id="A0A6I8UIV0">
        <property type="interactions" value="1446"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816462"/>
    <dbReference type="InParanoid" id="A0A6I8UIV0"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000002">
        <property type="entry name" value="Possible thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002497">
        <property type="entry name" value="PRK01827.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="11">
        <location>
            <begin position="46"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001356150.2"/>
        </source>
    </evidence>
    <sequence length="330" mass="37733" checksum="809480C30454CE00" modified="2020-08-12" version="1">MASAAAIPNLSSDNASPVKKSPIKRELKECNVEVPLLMQETNKDEQQYLDLIQYIIGNGERRIDRTNVGTLSVFGSQLRFDMRLSFPLLTTKRVFFRAVVEELIWFVGGKTDAKLLQAKNVHIWDGNSTREYLDKLGHTDRAVGDLGPVYGFQWRHFGAEYGTCDDDYSGKGIDQLRQVIDTIRHNPSDRRIIMSAWNPLDIPKMALPPCHCLAQFYVSQTRGELSCQLYQRSADMGLGVPFNIASYALLTYMIAHVTGLKTGDFIHTMGDAHVYLNHVDPLKEQLKRKPRPFPKLNIKRQVENIEDFRYEDFEIIGYEPYPKIKMDMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJW9</accession>
    <name>A0A6I8UJW9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extensin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356705.2">
        <property type="nucleotide sequence ID" value="XM_001356669.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJW9"/>
    <dbReference type="KEGG" id="dpo:4817476"/>
    <dbReference type="InParanoid" id="A0A6I8UJW9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJW9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026133264" evidence="1">
        <location>
            <begin position="26"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356705.2"/>
        </source>
    </evidence>
    <sequence length="327" mass="34083" checksum="D97C6F167722E604" modified="2020-08-12" version="1">MPHHRLSALGLVLLSFLLQLQSIRADVGYHYDRPSAPSAPASVYSGHQFTALPTIHPLPPIPSLPALPTARVPIPRIRPTAPAANIVSHYLPPKPSGPIVSTYLPPPVASPISSISFHGQTPTLRPVYGPPGYGFSQPLAPTASTPIVVPSGPGPVSAGGPPLRIPFGKQALISPGESYVANGRQLKQYAVIEIIDNDIDESPAPFLSSSSFFDRFGAHLGASSGPSNGVQLDSRANSLLLEQQSLSIQPRSQGGQGDAIALGSGGLGFVRLANGNVYLGSGSLGYISGQQRVASVLDARTRSDSTPDALHFGHGPLGGAENFLRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKB7</accession>
    <name>A0A6I8UKB7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">G surface protein, allelic form 168</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4817384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356883.4">
        <property type="nucleotide sequence ID" value="XM_001356847.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKB7"/>
    <dbReference type="KEGG" id="dpo:4817384"/>
    <dbReference type="InParanoid" id="A0A6I8UKB7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKB7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008472">
        <property type="entry name" value="DUF753"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21721:SF27">
        <property type="entry name" value="GH09876P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21721">
        <property type="entry name" value="GH09876P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05444">
        <property type="entry name" value="DUF753"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026343383" evidence="2">
        <location>
            <begin position="26"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="91"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356883.4"/>
        </source>
    </evidence>
    <sequence length="423" mass="44389" checksum="ABB23D9B3B6A01BE" modified="2020-08-12" version="1">MGSHTLFTALIVAALGLSQLDSCLAIQCYNCTSTDNGENCLKNPASFSTVDCTNDCFTSAEPGNITRGCLSGDANCSGFNCNSCNSDKCNTNLVCQQCLGQAECGSSNVTDTKYNVVCGSTDQVCVNQVNENQTVTRQCGSACATDAKPDTCSSCNTALCNEGMYPTTRLQCYSCTGETCNTPAITSLTACELVSAQCYMTGTSATAMQRGCTSDAGAKCQVGSTDTSCVICNTTSCNNLAYERDAGTCIECTNCAAEQNTTIAKSCGKLTYNQEDLGCYTLNNGTVTRGCLTAEQGACTTENSCKKCSEANCNVAAEAVEFQCAMCSTLTDAKCQAGVDAPLTACTSNNCFYGYWNGAGIRGCFDQASELMQYQCVNEKSHECVQCTTSLCNKVPFSGADTLKKLSVGLLVGLVLALRYTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL70</accession>
    <name>A0A6I8UL70_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 115</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4818131</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357285.3">
        <property type="nucleotide sequence ID" value="XM_001357249.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL70"/>
    <dbReference type="FunCoup" id="A0A6I8UL70">
        <property type="interactions" value="1969"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4818131"/>
    <dbReference type="InParanoid" id="A0A6I8UL70"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UL70">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000004">
        <property type="entry name" value="Transmembrane protein 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013861">
        <property type="entry name" value="TMEM115/Pdh1/Rbl19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377">
        <property type="entry name" value="PLACENTAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08551">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01160">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="306"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="411"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="328"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001357285.3"/>
        </source>
    </evidence>
    <sequence length="449" mass="49544" checksum="936EA09FC6507ECE" modified="2020-08-12" version="1">MSAPLARNWPYIWQQLNALLHNTSPVITLICVVTTFGYLLSFSDQAILLLSVTPGYILPNGKFWIWTAFTFCFIELHWWEVAVDVVTVGLCGKMLEPLWGQLEMFKFFALSNFGVSLLTTIYYLFYYMVTKNPTILFDVHIHGLAGYVAGICVAVRQIMPDHLIFKTRYGRLTNRNVPLTVLIMAIISWAIGLLDGTYPAMFASGSLVSWIYLRFYQHHPNGRGDSSESFTFVSFFPNVSQPFISVLVNPIYNCCLRAGVVKTPTPLRTISTASLTSISVQMPGADPHDIERRRQIALKALSERLKATDSTRHSQLPKSYPQQPQLQHHQHQQHQHHQQQKHHSHGAGHSHSHGAGHSHSHSGGGHSHGPGPAQTPQPDFLKPASNSAQMPITTSRAEPRMISTMSTIAIPMPAPPLKDGTGAAAGTDPQDGGATLINLDDVPTTSSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULB7</accession>
    <name>A0A6I8ULB7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Immunoglobulin-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Tap42</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IGBP1/TAP42 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357338.3">
        <property type="nucleotide sequence ID" value="XM_001357302.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULB7"/>
    <dbReference type="FunCoup" id="A0A6I8ULB7">
        <property type="interactions" value="800"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817950"/>
    <dbReference type="InParanoid" id="A0A6I8ULB7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULB7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.540:FF:000003">
        <property type="entry name" value="Immunoglobulin (CD79A)-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357338.3"/>
        </source>
    </evidence>
    <sequence length="374" mass="42466" checksum="D5CF062CC5253E86" modified="2020-08-12" version="1">MADENTAAAAAGGEDKKLSDIFLSGWNLYEELDVSELPFNGSEFQNKVKTALGHFEEATVIVNQVGMFSPNELIDEVSTDSLQFMLLPYFLGKLTTKINNPNSTEILDLAEIYFKDHLQRCQEYELCEAPKGTVQEDNKVEKSEHRELMEAAFNRNDKIAQYRKMKEIDEYMGRMRAAVQNKTADDEVRREFFLKYLDKSIIDSKHELESLCMMQKLSKMRIARLAGGEATTDDDTPSTSGKPSGSRGHGHGHSHHHHHAPKPKPLQPFIITRNATQKAVFGLGYPSLPVMSVDEFYQQRVDEGIFPDAEKSAKINQAQAIAAARDPNEKEDEEKAVEDQLIEEDDPENLARMRRMDEYKDVVRRGDGNRHNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULG3</accession>
    <name>A0A6I8ULG3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4817969</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
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        <property type="entry name" value="Alkaline phosphatase-like"/>
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</entry>
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            <fullName evidence="13">Protein ref(2)P</fullName>
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        </alternativeName>
    </protein>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="21">MV-25-SWS-2005</strain>
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            <end position="535"/>
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            <begin position="250"/>
            <end position="263"/>
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        <source>
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</entry>
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    <accession>A0A6I8ULK6</accession>
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                <consortium name="RefSeq"/>
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    <dbReference type="FunFam" id="3.30.160.20:FF:000120">
        <property type="entry name" value="RISC-loading complex subunit TARBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051247">
        <property type="entry name" value="RLC_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205:SF3">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="151"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="263"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="405"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="11"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357331.3"/>
        </source>
    </evidence>
    <sequence length="477" mass="52436" checksum="617A80A35051FB9A" modified="2020-08-12" version="1">MEQSNYHGRGLVQQLQNLSFQQNQNQVHQMQRPHQQHSPQLPHEQHPQHPQQQQQQQPLPAQPAQTGFAPRRQYDTSGGSAAAMAAGGLTNGNVDGTSTRTQRPSKVKKEKPQQPQQQIQLQLSEVDAASSTDEAIAFVSETDANGLAMKTPVSILQEILSRRGITPGYELVQIEGAIHEPTFRFRVSFKEKDTPFTAMGAGRSKKEAKHAAARALIDKLMGAQLPEAASSATATSAADPTAAGSGGDCNTAVGDPDDKIVGNPIGLLQELCMQRRWPPPTYATETEMGLPHERLFTIACTILQYREIGKGKSKKIAKRSAAHKMWSRLQDTPIDAAKVLDVCNELEGDSRINDNYYGDLKDITVPTLTTQHSNKVSQFHKTLKNATGKKLLKLQKTCLKNTTVDHPKLLTDIATENQFEVTYVDIEEKTFTGQFQCLVQLSTLPVGVCHGTGPTAREAQRHAAQNALEYLKIMTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULS4</accession>
    <name>A0A6I8ULS4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dihydrolipoyllysine-residue acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Pyruvate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">muc</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001357443.3">
        <property type="nucleotide sequence ID" value="XM_001357407.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULS4"/>
    <dbReference type="FunCoup" id="A0A6I8ULS4">
        <property type="interactions" value="1729"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4818036"/>
    <dbReference type="InParanoid" id="A0A6I8ULS4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULS4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006257">
        <property type="entry name" value="LAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01349">
        <property type="entry name" value="PDHac_trf_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="8">
        <location>
            <begin position="83"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="180"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357443.3"/>
        </source>
    </evidence>
    <sequence length="513" mass="54488" checksum="213DB049ABF2CD16" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULS5</accession>
    <name>A0A6I8ULS5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4800336</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357576.1">
        <property type="nucleotide sequence ID" value="XM_001357539.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULS5"/>
    <dbReference type="FunCoup" id="A0A6I8ULS5">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="GeneID" id="4800336"/>
    <dbReference type="KEGG" id="dpo:4800336"/>
    <dbReference type="InParanoid" id="A0A6I8ULS5"/>
    <dbReference type="OMA" id="CTNADCQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070968">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357576.1"/>
        </source>
    </evidence>
    <sequence length="290" mass="31672" checksum="8DC531374434715A" modified="2020-08-12" version="1">MFWILRRTGAANFFNSLNNNCSFSKNNSNGSNHSNSTQDKYVKRPPRKAYGRLSAEADDVVSLGHKIDDPFDELDDFEMLGANSNAGCSSHANNGNGEPPGEANANVNVNGNGHANGCGNGNGNLSLSACTDADCQGNFVVESALCELGLCQAKGKTSKELAAAAAAGAPSTSKEINENTISRLHALAMADDDDDYDESLTCNVCDRAFHCHRQLASHQQKKRHFGCSGCDSLFPSLMLLEHHKEEFEHWSDEEMGRRVCCRRNRCDDDYFTDTDSFISDAESEDLERLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULS7</accession>
    <name>A0A6I8ULS7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4800252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357540.2">
        <property type="nucleotide sequence ID" value="XM_001357504.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULS7"/>
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        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="GeneID" id="4800252"/>
    <dbReference type="KEGG" id="dpo:4800252"/>
    <dbReference type="InParanoid" id="A0A6I8ULS7"/>
    <dbReference type="OMA" id="HRHDDEY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078714">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505:SF8">
        <property type="entry name" value="DPT-YFP REPRESSOR BY OVEREXPRESSION, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21505">
        <property type="entry name" value="MADF DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="2">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357540.2"/>
        </source>
    </evidence>
    <sequence length="347" mass="39250" checksum="04FCC72060A3FE0C" modified="2020-08-12" version="1">MNVDLRSYSRHWLTEFIEQYQEEECLWQPKHNDYSNHAARNKSYDRLVEKLKEVEPNPDRPMVVRKINSLRSAFRREYRKTTCKSDYETRLWYYDRLLFIADHKPKRHESGTKPKRELHISFDDEDSMEYEDESHHTTSHSQQMDNLGPTSSDEVEEVPATNNVVVSSEGATLSTLSVTPADCVALVKSEQHQHDAAAVQAHQQLVAHAAAQNSIAAAASQGHAVKVLEITSLDSNSQREIQQAVNSLEQHQQQMHLQQANGHGQAVPTIQIGREHYQPLFSNANTTAYTTAPQPPPHDEYDAIGVNVASKLRAVNPTQRIIAEKLISDVLFNAQLDNLTVNSAVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMJ4</accession>
    <name>A0A6I8UMJ4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PolE1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="RefSeq" id="XP_001357879.3">
        <property type="nucleotide sequence ID" value="XM_001357842.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UMJ4">
        <property type="interactions" value="1373"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800644"/>
    <dbReference type="InParanoid" id="A0A6I8UMJ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.342.10:FF:000019">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="17">
        <location>
            <begin position="1530"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1235"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1282"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1947"/>
            <end position="1989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1962"/>
            <end position="1976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001357879.3"/>
        </source>
    </evidence>
    <sequence length="2242" mass="257855" checksum="0E9130C505E827F5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNV7</accession>
    <name>A0A6I8UNV7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein 626</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4801441</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358535.2">
        <property type="nucleotide sequence ID" value="XM_001358498.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNV7"/>
    <dbReference type="FunCoup" id="A0A6I8UNV7">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801441"/>
    <dbReference type="InParanoid" id="A0A6I8UNV7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001325">
        <property type="entry name" value="zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF377">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358535.2"/>
        </source>
    </evidence>
    <sequence length="263" mass="30596" checksum="5D5D34ACD581447A" modified="2020-08-12" version="1">MPKLFCGFRSWDGTRQHGTVVPNVEPTTKRNHGSSRVIKNSANTNKKSKLTFHYGSIKPTDNWLKSDDLPIVERRKMELRQGNQLMDILPESESHEVSHTIWELEKPYQAPDAGKKKNGTKISYISVTKGNKRVWTKEKQSKLVPKPSVIVEPLPTKTTRRTKQKGELFHCSECEKIFNHSWMLVAHMRTHTGERPFTCPEPKCRKSFADRSNLRSHQRTKGHHSWDYQCGQCGKYFNQDNFLKRHTLEACRKYLLNITHKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP75</accession>
    <name>A0A6I8UP75_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Testis-specific serine/threonine-protein kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Tssk</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358531.1">
        <property type="nucleotide sequence ID" value="XM_001358494.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP75"/>
    <dbReference type="GeneID" id="4801436"/>
    <dbReference type="KEGG" id="dpo:4801436"/>
    <dbReference type="InParanoid" id="A0A6I8UP75"/>
    <dbReference type="OMA" id="MLNASMP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072933">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14080">
        <property type="entry name" value="STKc_TSSK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000943">
        <property type="entry name" value="testis-specific serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF102">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="28"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358531.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="34042" checksum="B399C919C6260CB8" modified="2020-08-12" version="1">MSKFNTSVSGRQLSTRSSDVDALAQRGYNVGHKIGEGSYATVITAGYSDDSGHGVNLACKIIDKARAPTDFVNKFFPRELDILTKIDHPNIIQIHSILQRGPKIFIFMRFAENGDLLSHIKKMGPIEEKQSKIWFLQMAKALRYLHNIDIAHRDLKCENILLSKRLNIKLADFGFARYCRDDGGIEMKSDTYCGSAAYAAPEVVSGRPYDPKLADAWSLGVILFIMMNAKMPFDDSNLTKLLEDQRNRKFAFRRKLQDSITAPAKATVSVLLEPESAARWNLREILKCAWLRSVDQPETTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPD0</accession>
    <name>A0A6I8UPD0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Organic cation transporter protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4801718</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358763.3">
        <property type="nucleotide sequence ID" value="XM_001358726.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPD0"/>
    <dbReference type="KEGG" id="dpo:4801718"/>
    <dbReference type="InParanoid" id="A0A6I8UPD0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17317">
        <property type="entry name" value="MFS_SLC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="42"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358763.3"/>
        </source>
    </evidence>
    <sequence length="578" mass="63866" checksum="1A0284F3CF45723A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPV3</accession>
    <name>A0A6I8UPV3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine--tRNA synthetase-like protein Slimp</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Slimp</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358815.3">
        <property type="nucleotide sequence ID" value="XM_001358778.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPV3"/>
    <dbReference type="FunCoup" id="A0A6I8UPV3">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801776"/>
    <dbReference type="InParanoid" id="A0A6I8UPV3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000157">
        <property type="entry name" value="Seryl-tRNA synthetase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine-tRNA synthetase type1 N-terminal" evidence="4">
        <location>
            <begin position="53"/>
            <end position="149"/>
        </location>
    </feature>
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        <location>
            <begin position="85"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="298"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358815.3"/>
        </source>
    </evidence>
    <sequence length="466" mass="52608" checksum="1F226569B4CC4A94" modified="2020-08-12" version="1">MLGLQIVLRSCRAAQKGSIRKISALYITGDKANENYATLQPYLDFAGTFDEAASLEQSIASRGLDISLESVFRKYQKYQTHHEQLQRVVGEREAVTKNLKELTKKGGSEQQIEELKEQGKTLRNDLKGLKQALYPIEDEFIHDFLHLPNRLHAQCPTGDQEVLLYRHSLPGSTSNVPSHLDRKDLIHFVDNNRYYMMEQAAHFDVNAMQSLGRYFVTKGDFVQTANPDFVRCVLLEANATPLADYHMVREEHLQNKINTAYLTGGAAFESYLGAMTKLCVYPSVLPLRYVCCGRSYNRAEAQHYGPTPSLYTATQTNAVQSFVATQTADEANAQLEHILSLAIDFYKALDVPFRIVYAPAACLTPSESLRAVIEVYAPSLQRYVCVGRISNYGDFVSKRILFSTRREKHYDFLHLVGGPVLYTTRLIAALLEHNIRLENCRLVGASSSGGKHTMEQDLQQFKDLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQM5</accession>
    <name>A0A6I8UQM5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enhancer of split m6 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">E(spl)m6-BFM</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359176.3">
        <property type="nucleotide sequence ID" value="XM_001359139.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQM5"/>
    <dbReference type="KEGG" id="dpo:4802208"/>
    <dbReference type="InParanoid" id="A0A6I8UQM5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001359176.3"/>
        </source>
    </evidence>
    <sequence length="76" mass="8786" checksum="5493F21AA8A536CC" modified="2020-08-12" version="1">MSKVKNLLAKIFQRFGRGHNNCHGGSNDSQRYDSLEEIAQNQANERMLMETLIRCEEEHLVICLETAAGSFYWHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR61</accession>
    <name>A0A6I8UR61_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359410.4">
        <property type="nucleotide sequence ID" value="XM_001359373.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR61"/>
    <dbReference type="KEGG" id="dpo:4802500"/>
    <dbReference type="InParanoid" id="A0A6I8UR61"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359410.4"/>
        </source>
    </evidence>
    <sequence length="486" mass="54753" checksum="5BF18B74A35A54B0" modified="2020-08-12" version="1">MTTLESDKEKLCCLIYCIGILTISAAIWNICKLAGKYFNKMTTKEEVTLKQRQAADDLTKRLRHYLVNGPNQSEICGKVVLAMEQVNIVPLLVGNGIEKAKLRSILHVLAQRMRCLRVKMADIRRLHDLCHRMGLSELANVRVCIVLADDKGNSSAGGSHLELLGSLTPGVEILSVLCCIDGHFPELKKVRIVHLSCTVTQRTLEELFRKCPLLQHFKLKNEGRSPEIMDMKNIGQCSLLEGLLLPLLLMSPLHVCQLSNLKQLTLQSKDQWQNEEWLLVVQEIIKAKRFELERICFDGAYVGAPLNLSVLLLARCTALTEVRLTRCNLVGDGKLPPPLPFSCHCLRIRHCSLPKLSLLLASDCMLQHMDLFNCKSVKTANLLAEALAMRKSQACISPLVIKLFQSPELEIEYNKWSPQEQLAAKPWLEIEVLKKDHIPSWDDQRLGLISMQFGQAINSFPTSLEDDQNQLENADLLKEMDSVCGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URI3</accession>
    <name>A0A6I8URI3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-uridine aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Dtwd2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62432"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:16092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82930"/>
            <dbReference type="EC" id="2.5.1.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TDD superfamily. DTWD2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.25" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001359754.3">
        <property type="nucleotide sequence ID" value="XM_001359717.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URI3"/>
    <dbReference type="FunCoup" id="A0A6I8URI3">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="GeneID" id="4802939"/>
    <dbReference type="KEGG" id="dpo:4802939"/>
    <dbReference type="InParanoid" id="A0A6I8URI3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070092">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016432">
        <property type="term" value="F:tRNA-uridine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005636">
        <property type="entry name" value="DTW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039262">
        <property type="entry name" value="DTWD2/TAPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21392">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21392:SF0">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03942">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01144">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DTW" evidence="7">
        <location>
            <begin position="22"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359754.3"/>
        </source>
    </evidence>
    <sequence length="255" mass="28431" checksum="DADE8AF2C8B258B0" modified="2020-08-12" version="1">MTTMEDDAWQDLIGISADPPDRRDKCEQCKRPAVVCWCPALPQPPLAVASQIVILQHPAEEKRSLRTALMLQLGLEPGKCVVYKGKRFPNHKNHAELQQIFDSPQTLVLYPSKDSVPLEEIDRSGGPYTLVLIDGTWPQAKAIYASSPALHRLRQVKLISVGTSDYIIRTQPTEGCLSTLETAAQCLAVLEAQPDLRQVLVRPLHALCKYQLDNGAVEHQSKEFLMKTNQYPKPIGKRLSRLLNQTVCGSAEKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URM2</accession>
    <name>A0A6I8URM2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4802749</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the clarin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359610.2">
        <property type="nucleotide sequence ID" value="XM_001359573.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URM2"/>
    <dbReference type="KEGG" id="dpo:4802749"/>
    <dbReference type="InParanoid" id="A0A6I8URM2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070772">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026748">
        <property type="entry name" value="Clarin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548">
        <property type="entry name" value="CLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548:SF1">
        <property type="entry name" value="LD47387P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359610.2"/>
        </source>
    </evidence>
    <sequence length="242" mass="27035" checksum="EF102A134839929E" modified="2020-08-12" version="1">MVKTLLLPPVITSFIKMSMRTRALVFSTFFGSCLAIGLLLVSMTTNHWVRANPRRHTSSKAKGDVNFGLFYGNHHLDLGFGVRTYPVDVYTFIRTENGDTSFWLWLLTTLGTGFGLLACAVAAIAAVLKSASAAKRGGTMVLLLTANISAAGAQVVAFVAWLVQFYQYLIHNVLLTEHQQQHWYSTGLASLGYSFYLVVISTIVVLLNITILLYARRRESRDRRLRLEPESKDKHQAAIMLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URT8</accession>
    <name>A0A6I8URT8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4803098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359896.3">
        <property type="nucleotide sequence ID" value="XM_001359859.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URT8"/>
    <dbReference type="FunCoup" id="A0A6I8URT8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803098"/>
    <dbReference type="InParanoid" id="A0A6I8URT8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359896.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="659" mass="72344" checksum="FDC1538F1DCCB177" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USD3</accession>
    <name>A0A6I8USD3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4803454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360175.1">
        <property type="nucleotide sequence ID" value="XM_001360138.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USD3"/>
    <dbReference type="FunCoup" id="A0A6I8USD3">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="GeneID" id="4803454"/>
    <dbReference type="KEGG" id="dpo:4803454"/>
    <dbReference type="InParanoid" id="A0A6I8USD3"/>
    <dbReference type="OMA" id="IVYVWIN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071900">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF521">
        <property type="entry name" value="IP01817P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="141"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360175.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="25133" checksum="D5265B9220832805" modified="2020-08-12" version="1">MDCGGVFVKYVLFIFNILFVICGILLITFGSIMVSTIKDFSSMDETFTANSVAIIILVLGCLIFLVAFMGCCGAIREDACCLTTYSSVMMVLLISQLVLIIYVWVDHVQIQQSLEKIVETIWQQRKTDSILLDTMQRTFKCCGLHRFSDYGVSYPASCCDSPSNGTCGLTSVMLRPGCLDAVDSFWDTNVNIIKYAGLGVTAIELVAFIFGCCLANQTRNSARRQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USU7</accession>
    <name>A0A6I8USU7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">mRNA-decapping enzyme 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DCP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360227.2">
        <property type="nucleotide sequence ID" value="XM_001360190.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USU7"/>
    <dbReference type="FunCoup" id="A0A6I8USU7">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="GeneID" id="4803514"/>
    <dbReference type="KEGG" id="dpo:4803514"/>
    <dbReference type="InParanoid" id="A0A6I8USU7"/>
    <dbReference type="OMA" id="NASIFNM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070879">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09804">
        <property type="entry name" value="Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000425">
        <property type="entry name" value="mRNA-decapping enzyme 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031953">
        <property type="entry name" value="mRNA_decap_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16741">
        <property type="entry name" value="mRNA_decap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA-decapping enzyme C-terminal" evidence="6">
        <location>
            <begin position="306"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001360227.2"/>
        </source>
    </evidence>
    <sequence length="343" mass="38721" checksum="326024B8DA879774" modified="2020-08-12" version="1">MADESITRMNLSAIKKIDPYAKEIVDSSSHVAFYTFNSEQNEWEKTDVEGAFFIYHRNAEPFHSIFINNRLNTTSFVEPITGSLELQSQPPFLLYRNERSRIRGFWFYNSEECDRMNGVVNSLLQNKESNGLVANPNAAPTFVIPKHDNASIFNMLSKAQKEYNAQCNNQSKSMQPENVTPGNVLKFFESAKQATAESGTVQYNRVQPLSVDQLEKQQRAATPNEGLIPTREAPSECRLSPFQKPSLNAQPLPLLSELKIGSPLMANEDAGNCLRRLLVGDQPEAKPALMPPSMFDAPNGNPEPVQPLSSSRFVQAFTYLIQNDKEFVNKLHRAYLKFDEDSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USX0</accession>
    <name>A0A6I8USX0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Coenzyme Q-binding protein COQ10, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4803421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360136.1">
        <property type="nucleotide sequence ID" value="XM_001360099.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USX0"/>
    <dbReference type="FunCoup" id="A0A6I8USX0">
        <property type="interactions" value="471"/>
    </dbReference>
    <dbReference type="GeneID" id="4803421"/>
    <dbReference type="KEGG" id="dpo:4803421"/>
    <dbReference type="InParanoid" id="A0A6I8USX0"/>
    <dbReference type="OMA" id="IDGPFKY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081751">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="4">
        <location>
            <begin position="56"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360136.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="24134" checksum="A962757F9743EA82" modified="2020-08-12" version="1">MLKGSKLKALDVRILRPLFETSCCQQRSFANSIQRSYITFNDFRSKNRWYTKKELVGYSMKDMYTVVSDVRNYYKFVPYVKRSHVHTVDSDGFKADLIVGFPPLNEAYTSRVTLESPSLVKSECHDGRLFNYLLNEWRFSPGLKDIPNSCVLDFKVSFEFKSLLHSNVANIFFDLICDQMENAFIEEVRRRNGPPSIRSHVLTSQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT00</accession>
    <name>A0A6I8UT00_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Vps51</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the Golgi-associated retrograde protein (GARP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the VPS51 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360415.2">
        <property type="nucleotide sequence ID" value="XM_001360378.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT00"/>
    <dbReference type="FunCoup" id="A0A6I8UT00">
        <property type="interactions" value="882"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803750"/>
    <dbReference type="InParanoid" id="A0A6I8UT00"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007041">
        <property type="term" value="P:lysosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014812">
        <property type="entry name" value="Vps51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360415.2"/>
        </source>
    </evidence>
    <sequence length="738" mass="83578" checksum="69722046AD19B2D0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT35</accession>
    <name>A0A6I8UT35_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">YY1-associated factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RYBP</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360490.1">
        <property type="nucleotide sequence ID" value="XM_001360453.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT35"/>
    <dbReference type="SMR" id="A0A6I8UT35"/>
    <dbReference type="FunCoup" id="A0A6I8UT35">
        <property type="interactions" value="1996"/>
    </dbReference>
    <dbReference type="GeneID" id="4803837"/>
    <dbReference type="KEGG" id="dpo:4803837"/>
    <dbReference type="InParanoid" id="A0A6I8UT35"/>
    <dbReference type="OMA" id="TADHHSQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071517">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039958">
        <property type="entry name" value="RYBP/YAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033774">
        <property type="entry name" value="YAF2_RYBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12920:SF4">
        <property type="entry name" value="GEO03726P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12920">
        <property type="entry name" value="RYBP AND YAF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17219">
        <property type="entry name" value="YAF2_RYBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="10">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="64"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001360490.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="16829" checksum="D89EF764DCEB3EBA" modified="2020-08-12" version="1">MDKKSSPVRRQKRQAKVIEDNFWDCSVCTYRNSAEAFKCRMCDVRKGTSTRKPRLNSALVAQQAATLPGASGNMPNGKSASSSRHGSGHDRQRHKRYPARLKNVDRSTAQTREVTVNSVTVFITEYKAKPVSSRRESSEQSFSESNDSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU52</accession>
    <name>A0A6I8UU52_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">TWiK family of potassium channels protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">sand</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360922.2">
        <property type="nucleotide sequence ID" value="XM_001360885.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU52"/>
    <dbReference type="FunCoup" id="A0A6I8UU52">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="GeneID" id="4804348"/>
    <dbReference type="KEGG" id="dpo:4804348"/>
    <dbReference type="InParanoid" id="A0A6I8UU52"/>
    <dbReference type="OMA" id="TPQSEWG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081263">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF352">
        <property type="entry name" value="BCDNA.GH04802-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="347"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="124"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="294"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="225"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001360922.2"/>
        </source>
    </evidence>
    <sequence length="396" mass="45216" checksum="09D80FAABD69B254" modified="2020-08-12" version="1">MSSRRSSFRRKEKPAFQRFKDHCRHFTAFMFSNVGIILLVTFYTIGGAFIFQAIEIFEYERLRSEKPHRFIERNVSGECLTRIWELTAENISFFDHKAYRKRVNDVLLEYQRAIVKKQLKGPDVEQWSFSGAFLYSLTVITTIGYGNITPHSEWGKLATILYAIIGMPLFLLYLSNIGDVLAKSFKWIYSKVCLCRICPGVAKRRIIRERRKMRHLARALQMHHMESGGGSSSYSSSSSTTNSNSSSSTDYTKSSYRSSSIVDVAYSGSDSDIEREIRGSTDEITVPLTVCIFVMVGYILWGALLFGRWEDWNYLDGSYFCLISLSSIGFGDLVPGDRVITADRDKVEVSFILCAVYLLLGMAVIAMCFNLMQEEVVHNIRAIKRGFKACFRCRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUC0</accession>
    <name>A0A6I8UUC0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase II-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">spag</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the RPAP3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360908.3">
        <property type="nucleotide sequence ID" value="XM_001360871.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUC0"/>
    <dbReference type="FunCoup" id="A0A6I8UUC0">
        <property type="interactions" value="1319"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804332"/>
    <dbReference type="InParanoid" id="A0A6I8UUC0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UUC0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051966">
        <property type="entry name" value="RPAP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025986">
        <property type="entry name" value="RPAP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423:SF1">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13877">
        <property type="entry name" value="RPAP3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-polymerase II-associated protein 3-like C-terminal" evidence="7">
        <location>
            <begin position="455"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="293"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="393"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360908.3"/>
        </source>
    </evidence>
    <sequence length="573" mass="63859" checksum="5B7A8EBBD830CADC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUD7</accession>
    <name>A0A6I8UUD7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lysozyme</fullName>
            <ecNumber evidence="2">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4804179</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001360782.4">
        <property type="nucleotide sequence ID" value="XM_001360745.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUD7"/>
    <dbReference type="KEGG" id="dpo:4804179"/>
    <dbReference type="InParanoid" id="A0A6I8UUD7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16890">
        <property type="entry name" value="lyz_i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000019">
        <property type="entry name" value="lysozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008597">
        <property type="entry name" value="Invert_lysozyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11195">
        <property type="entry name" value="DESTABILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11195:SF22">
        <property type="entry name" value="LYSOZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05497">
        <property type="entry name" value="Destabilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51909">
        <property type="entry name" value="LYSOZYME_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="4" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026309024" description="lysozyme" evidence="8">
        <location>
            <begin position="27"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="36"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="41"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="90"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608597-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360782.4"/>
        </source>
    </evidence>
    <sequence length="175" mass="19373" checksum="0D715DD3E4F0C534" modified="2020-08-12" version="1">MLASLKLLTSLWLLLWLRLDSVPGHAVENKPVTEDCLECLCETMSGCNATAICVNGACGIFRITEGYWVEGGKLTLPDETPLSNRAFINCVNQPLCAANTIQSYMYKHGQDCNGDDHIDCLDFGALHKLGNLKCRGELPYIYAKVFNSCLKNKERQAQNADQTPNQVKIKDQSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVG4</accession>
    <name>A0A6I8UVG4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein FAM8A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4805036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361503.2">
        <property type="nucleotide sequence ID" value="XM_001361466.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVG4"/>
    <dbReference type="FunCoup" id="A0A6I8UVG4">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805036"/>
    <dbReference type="InParanoid" id="A0A6I8UVG4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039871">
        <property type="entry name" value="FAM8A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13659">
        <property type="entry name" value="AUTOSOMAL HIGHLY CONSERVED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13659:SF5">
        <property type="entry name" value="PROTEIN FAM8A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="6">
        <location>
            <begin position="115"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361503.2"/>
        </source>
    </evidence>
    <sequence length="320" mass="36835" checksum="3F2F9200D7339BFA" modified="2020-08-12" version="1">MSEQIEEKLQKLFTDPTVSQDLTTKEAYFASLSEWAKQASISQNAMAMFPYYLLANYPQLFQTNPLLSSPPLRAQETGNAPAPAAVPPAFHRFRVLDEIQQQETIQRVGGYEYVVAPFWKRAVAEAIDMLILFILKIIVTFGIVNLFEIDFDKDVMRRTLEDEDLFVNFFDLSMDFINMSSDLLMIEMLTKMIVCCYEAVWTVWYNGATPGKSLMKIRIHYVEAVLPLQAPVLPQFVFQPQREPLRALLYPAETPKLLRAFARALAKNLVMTLLFPICVVMIFFKNNRTAYDIMTKTIVVESNSNAIYRTDVPQQQQRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVN7</accession>
    <name>A0A6I8UVN7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein scabrous</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sca</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361633.3">
        <property type="nucleotide sequence ID" value="XM_001361596.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVN7"/>
    <dbReference type="FunCoup" id="A0A6I8UVN7">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805186"/>
    <dbReference type="InParanoid" id="A0A6I8UVN7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF444">
        <property type="entry name" value="PROTEIN SCABROUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026302133" evidence="4">
        <location>
            <begin position="24"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="5">
        <location>
            <begin position="530"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="258"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="738"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="749"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361633.3"/>
        </source>
    </evidence>
    <sequence length="786" mass="87407" checksum="8EAFF5BA55794FCA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW58</accession>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4805406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361811.3">
        <property type="nucleotide sequence ID" value="XM_001361774.4"/>
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    <dbReference type="KEGG" id="dpo:4805406"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="Ganglioside GM2 activator"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GM2-AP_sf"/>
    </dbReference>
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        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026218302" evidence="2">
        <location>
            <begin position="18"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361811.3"/>
        </source>
    </evidence>
    <sequence length="176" mass="20512" checksum="E84732A9E4453C59" modified="2020-08-12" version="1">MLLLCLLLLHEAPLAHPYKEHLQSTFTNVSVEYSPEFCGSIRGWLTPQGNLNLDIHLNRTLRQGLRTAITLQQRVGGGYQTLFSYDLDTCRTLGELLQSSLMKVWLRNVFRYGHLATRCPIQPAHYDLRDFQLENHSIPGYLRPGLFRLNDTNYYRRPRGGRRRLVATFTLDIKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWF8</accession>
    <name>A0A6I8UWF8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4805504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361897.3">
        <property type="nucleotide sequence ID" value="XM_001361860.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWF8"/>
    <dbReference type="KEGG" id="dpo:4805504"/>
    <dbReference type="InParanoid" id="A0A6I8UWF8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361897.3"/>
        </source>
    </evidence>
    <sequence length="79" mass="8934" checksum="14927D18B5237CB2" modified="2020-08-12" version="1">MDSVKSFFAGSSKGNNSLGNHQQQNGHGSESTNRWGRNWNSSSQHMDTSSTNSTSINKTNHCDAKRKDSDNYFYIMWRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX96</accession>
    <name>A0A6I8UX96_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Ugt307A1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="5"/>
    <dbReference type="RefSeq" id="XP_002132484.2">
        <property type="nucleotide sequence ID" value="XM_002132448.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX96"/>
    <dbReference type="FunCoup" id="A0A6I8UX96">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903296"/>
    <dbReference type="InParanoid" id="A0A6I8UX96"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000021">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF145">
        <property type="entry name" value="FI06409P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026378368" description="UDP-glucuronosyltransferase" evidence="5">
        <location>
            <begin position="25"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002132484.2"/>
        </source>
    </evidence>
    <sequence length="505" mass="57486" checksum="7D6FAFEDA1FDE799" modified="2020-08-12" version="1">MNRPPVSHILLWMLAICSAVGVESHHILGLFVNLHRSQLLVHMAVTRALLQRGHQLTLVTTLPLEEVELEGNVTHILVPWQQPADEDHLGSATDWLLRLQRMFNRLEQSGDLLDQPAWKDFMRLSPKPHFDLLLLGYHFNDHLLGVAAHFNCPAAIISTQQPIGFVNSLMGNPEERWYVPQPYDSRQRSGISAWLFGAWEKLMEAVARRVLRRIYSSHFPEPHYPPFETMRRSVALALNNHHMISEGPIAPLLPSMVDIGGILLEQKLNETFLEVSGNRSLIVFSLGTRFTWKRSPDHFVQTFTQAFAQFPDYDIYWTYDGPNASEIMSHHSHLKLAKWWPQRQLLGQQQACLFITHGGKGSLTEALYYGVPMVGLPLMGDQRANLLKMGAKGWGMTLSTQNLSHAELSKAMSSALSHSRYSKSISKASHLYRDRPMNASDVATFWLEYVIRHRGAKHLYSPARQLDFMQYYSLDVYAVVYGGLVLLIAVLRRINSRLSVTRSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXB9</accession>
    <name>A0A6I8UXB9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Venom allergen-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4805718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001362073.2">
        <property type="nucleotide sequence ID" value="XM_001362036.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXB9"/>
    <dbReference type="SMR" id="A0A6I8UXB9"/>
    <dbReference type="FunCoup" id="A0A6I8UXB9">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="GeneID" id="4805718"/>
    <dbReference type="KEGG" id="dpo:4805718"/>
    <dbReference type="InParanoid" id="A0A6I8UXB9"/>
    <dbReference type="OMA" id="YGHFAET"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082043">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05380">
        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000007">
        <property type="entry name" value="Venom allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034763">
        <property type="entry name" value="P14a_insect"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038921">
        <property type="entry name" value="P14a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026138344" description="Venom allergen-1" evidence="6">
        <location>
            <begin position="20"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="7">
        <location>
            <begin position="60"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001362073.2"/>
        </source>
    </evidence>
    <sequence length="263" mass="29934" checksum="868046EF9E7A5F31" modified="2020-08-12" version="1">METGKYLLISVLLMPMAIGQPLSWCDPQLCPEGKVHIACNNNGKFHETCSEDATMVNLKPHRSFILNEHNKRRNFIASGSLPGYFPATRMATMVWDEELEYLATLNLKTCNLEHDDCNHSFRFRNLGQNLCGVDRYRSAPMNVTNLVEQSLGLWFGEHHVIDSSFITDFRVTSKLEKYGHFVETVVDRNTHVGCAMMRFTNPKYPFLYIYNTACNYASVYAIGVPVYNAGLPASECLTGNNPQYPALCSVKEKYNPNYNIYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY13</accession>
    <name>A0A6I8UY13_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Elastin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132984.2">
        <property type="nucleotide sequence ID" value="XM_002132948.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY13"/>
    <dbReference type="KEGG" id="dpo:6902439"/>
    <dbReference type="InParanoid" id="A0A6I8UY13"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UY13">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132984.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="19915" checksum="B58C8ECE9FB4F4C9" modified="2020-08-12" version="1">MNQENKPPMTWVPAGQGGSFVGSTWGIPPYARAVFVPAGQGEPYAGAWVPNGLIEQTVGACVPAILIAPSAGVWVSTGQSGPYAGVWVPVGLIGPYAGALVPAGMNGPYASALGPTGQGAPYAGPWVPNGRIEPSSGSWGSTGQAGRPAPQNRHQKRQLLRSTYVPYSHQLEKEFEEDVAAYSNISSVDKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYI4</accession>
    <name>A0A6I8UYI4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6901943</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133477.2">
        <property type="nucleotide sequence ID" value="XM_002133441.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYI4"/>
    <dbReference type="FunCoup" id="A0A6I8UYI4">
        <property type="interactions" value="1560"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901943"/>
    <dbReference type="InParanoid" id="A0A6I8UYI4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000133">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="9">
        <location>
            <begin position="86"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002133477.2"/>
        </source>
    </evidence>
    <sequence length="268" mass="28696" checksum="F377BECD5489164E" modified="2020-08-12" version="1">MADAAPARGGFRGGFGSRGGRGGRGRGRGRGARGRGGKEDSKEWVPVTKLGRLVREGKIKSLEEIYLYSLPIKEFEIIDFFLGSALRDEVLKIMPVQKQTRAGQRTRFKAFVAIGDNNGHIGLGVKCSKEVATAIRGAIILAKLSVVPVRHGYWGNKIGKPHTVPCKVTGKCGSVSVRLIPAPRGTGIVSAPVPKKVLTMAGIEDCYTSARGSTGTLGNFAKATYAAIAKTYAYLTPDLWKEMTLGSTPYQAYSDFLSKPTARLHADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYW1</accession>
    <name>A0A6I8UYW1_DROPS</name>
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        <submittedName>
            <fullName evidence="8 9">Protein lozenge</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">lz</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002133775.2">
        <property type="nucleotide sequence ID" value="XM_002133739.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015185847.2"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001709">
        <property type="term" value="P:cell fate determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048592">
        <property type="term" value="P:eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.720:FF:000001">
        <property type="entry name" value="Runt-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000040">
        <property type="entry name" value="AML1_Runt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="Runt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950:SF49">
        <property type="entry name" value="PROTEIN LOZENGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950">
        <property type="entry name" value="RUNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00853">
        <property type="entry name" value="Runt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00967">
        <property type="entry name" value="ONCOGENEAML1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51062">
        <property type="entry name" value="RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Runt" evidence="6">
        <location>
            <begin position="269"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="203"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="546"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="772"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="40"/>
            <end position="68"/>
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            <begin position="145"/>
            <end position="159"/>
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            <end position="224"/>
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        <location>
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    </feature>
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            <end position="512"/>
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        <location>
            <begin position="546"/>
            <end position="572"/>
        </location>
    </feature>
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            <begin position="577"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="795"/>
            <end position="812"/>
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        <location>
            <begin position="813"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="830"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51062"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002133775.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015041333.2"/>
        </source>
    </evidence>
    <sequence length="871" mass="89524" checksum="C0696763B40EBAA9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ29</accession>
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        <submittedName>
            <fullName evidence="7">Gametocyte-specific factor 1 homolog</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="primary">LOC6901217</name>
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    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133918.2">
        <property type="nucleotide sequence ID" value="XM_002133882.3"/>
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    <dbReference type="KEGG" id="dpo:6901217"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
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    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="5">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="CHHC U11-48K-type" evidence="5">
        <location>
            <begin position="49"/>
            <end position="76"/>
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    </feature>
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        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51800"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002133918.2"/>
        </source>
    </evidence>
    <sequence length="156" mass="18476" checksum="DE1857CABB64D427" modified="2020-08-12" version="1">MSQNLSLKVAPSNKDDYLECPFDERHLIMRSRMTVHLVRCAPNHHGSRKVRCPFNNTHIDTISKMKIHVGTCPERAGFEKFVHQNEIEEPDELPYEKREEIECSEDWDKEPDVGTYNPKLYCDKKFLILNPQGNPPAVKRQIRESERKRFQQHQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZH4</accession>
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        <submittedName>
            <fullName evidence="4">Protein CutA homolog</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC6901561</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
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        <property type="nucleotide sequence ID" value="XM_002134199.3"/>
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        <property type="interactions" value="342"/>
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        <property type="term" value="F:copper ion binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_tolerance_CutA"/>
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    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="DIVALENT CATION TOLERANCE CUTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419:SF8">
        <property type="entry name" value="FI09726P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026064350" evidence="2">
        <location>
            <begin position="36"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002134235.3"/>
        </source>
    </evidence>
    <sequence length="174" mass="19648" checksum="160D39BF123405BA" modified="2020-08-12" version="1">MQYRGNARFLPSRCLFARNLLIVAAAAVTSRSAFASTTTCKRILYSPTRMTDRQTCDSSSYKAGSSSVAFVTTPDRESAKKLAHGIIDRKIAACVNIIPKIESIYMWEGKVTEDDEYLMMIKTRTERIDELSKFVRENHPHSVAEVISIPIENGNLPYLNWIAQTVPELEKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZK6</accession>
    <name>A0A6I8UZK6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Gr10a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134181.2">
        <property type="nucleotide sequence ID" value="XM_002134145.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZK6"/>
    <dbReference type="FunCoup" id="A0A6I8UZK6">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901495"/>
    <dbReference type="InParanoid" id="A0A6I8UZK6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZK6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002134181.2"/>
        </source>
    </evidence>
    <sequence length="412" mass="48258" checksum="A60357D56F691A58" modified="2020-08-12" version="1">MSAAEEHEPKESFWERHEFKFYKYGHIYAVIYGQVVIDYVPQQPMRRGLKTVLIAYSHLLSLILIVVLPGYFVYHFRTLTETHDRRMQLMLYVSFANTAIKYATVIVTYVANTVHFEAINYRCTVQRQRLETAFVGAPKQPKRSFEFFMYFKFCLINLMMLVQVAGIFALYEAADGPSVSQVRLHFAIYAFVLWNYTENMADYFYFVNGSVLKYYRQLHLQLVSLRRQLGGLRPGGMLMEHCCRLSDRLGELRQRFGEIHDLYSESFQMHQFQLLGLVLATLINNLTNFYAIFNMLAKQSLEEISFPIVIGSVYATGFYIDTYIVTLLNEHIKQELEGFALTMRTFAEPPSTVEQRLTQEIEYFSLDLLKCRPPMLCGLLNFDRRLIYLIAVTAFCYFITLVQFDLYLRKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZS8</accession>
    <name>A0A6I8UZS8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Limbic system-associated membrane protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DIP-alpha</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134309.2">
        <property type="nucleotide sequence ID" value="XM_002134273.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZS8"/>
    <dbReference type="FunCoup" id="A0A6I8UZS8">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901640"/>
    <dbReference type="InParanoid" id="A0A6I8UZS8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZS8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000328">
        <property type="entry name" value="CLUMA_CG000981, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000376">
        <property type="entry name" value="CLUMA_CG000981, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF255">
        <property type="entry name" value="DPR-INTERACTING PROTEIN ALPHA, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026213251" evidence="10">
        <location>
            <begin position="33"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="38"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="140"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="240"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="336"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="380"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="382"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002134309.2"/>
        </source>
    </evidence>
    <sequence length="563" mass="60986" checksum="42C8D599F4AD84E8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2S0</accession>
    <name>A0A6I8V2S0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6897273</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_002137433.4">
        <property type="nucleotide sequence ID" value="XM_002137397.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2S0"/>
    <dbReference type="FunCoup" id="A0A6I8V2S0">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897273"/>
    <dbReference type="InParanoid" id="A0A6I8V2S0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026018814" description="procollagen-proline 4-dioxygenase" evidence="13">
        <location>
            <begin position="17"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="373"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002137433.4"/>
        </source>
    </evidence>
    <sequence length="491" mass="55836" checksum="5B0D51AB42ABAAFE" modified="2020-08-12" version="1">MISPYWLLLWLGLGAGEEQHYATSTRHLAFLLQVEDELLATVRQYAVELQQKVDTMRVLQAEWMARRVEARADPDSYVANPLRSLPLMRRMHVDARKWLDFARLEVGQESLKQLADFDLSGISPMDFQEAADGLLRIQEIYDLDERDMARGKLRHKHYNSRLSSADCLALGVHLEDLQKGRLSGKWLEVALEQYEDKWEPIHRLLQVGRSQIWQQLGLTLISTHDLQASHAAFTKAVEGASESEDVEIARHLADNLGYHFVHQRNCQGRSRLPVQSSLRCHYSAEGSAFLRLAPLRMELLSRDPLVAVYHEVVSAAEQRHLMLLSESQLQRQRGHQYDKIRTFASASVAANATPTVEQLHRRLEDITGLDLAESEPLRILNYGIGGQYYIHVDCEQPQTHVEPYPKEYRLATVLLYLSDVRLGGFTSFPALGLGIRPNRGSALVWHNANNAGNCDYRALHAACPVLLGTRWVASKWISGSGGQWRRKPCRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2T2</accession>
    <name>A0A6I8V2T2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC6897337</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002137490.2">
        <property type="nucleotide sequence ID" value="XM_002137454.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2T2"/>
    <dbReference type="KEGG" id="dpo:6897337"/>
    <dbReference type="InParanoid" id="A0A6I8V2T2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07851">
        <property type="entry name" value="STKc_p38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000684">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000028">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="20"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002137490.2"/>
        </source>
    </evidence>
    <sequence length="370" mass="42726" checksum="E7B7704B6BB168E4" modified="2020-08-12" version="1">MSKFTKFVMDEKAWEVPDVYEIERLLGAGSFGQVSKAKLRGGDVNVAIKKLLQPFETAEHAKRVYREIRLLKHMDHPNVISLLDVFHPSSPSPTLENFQQVYLVTHLMDADLHKTIRSQKLSDNQIRVILYQILRGLKYIHSAGVLHRDLKPGNIAVNKDCELRILDFGMARLNSKDMTTYVTTRWYRAPEILFCWRNYSKAIDMWSVGCIFAEMITGRPLFPGRDYTNQLDCIIDVMGTPSDEFKSKIDLESARTYVESLPRRTKSDFMELFGMGNSQAVDLIEKMLVMDPENRITADEALRHPFLRNLVQPQHNDEDTAPLYDQNFENMDWLSVKCWKELVLNEIMNFRPPPAYGEVLNSAMCQGDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3W3</accession>
    <name>A0A6I8V3W3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">List</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Unusual broad substrate spectrum amino acid:sodium cotransporter that promotes absorption of the D isomers of essential amino acids. Neutral amino acids are the preferred substrates, especially methionine and phenylalanine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138287.2">
        <property type="nucleotide sequence ID" value="XM_002138251.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3W3"/>
    <dbReference type="FunCoup" id="A0A6I8V3W3">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898221"/>
    <dbReference type="InParanoid" id="A0A6I8V3W3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V3W3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10324">
        <property type="entry name" value="SLC6sbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF321">
        <property type="entry name" value="SODIUM-DEPENDENT NUTRIENT AMINO ACID TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 16" id="KW-0769">Symport</keyword>
    <keyword evidence="4 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="130"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_002138287.2"/>
        </source>
    </evidence>
    <sequence length="556" mass="61098" checksum="20B0BBEF22B19EBE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4X5</accession>
    <name>A0A6I8V4X5_DROPS</name>
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        <recommendedName>
            <fullName evidence="11">Small ribosomal subunit protein mS39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6898770</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS39 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_002138712.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4X5"/>
    <dbReference type="FunCoup" id="A0A6I8V4X5">
        <property type="interactions" value="1952"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Protein PTCD3 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037387">
        <property type="entry name" value="PTCD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055063">
        <property type="entry name" value="Rib_mS39_PPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16276">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16276:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22330">
        <property type="entry name" value="Rib_mS39_PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002138748.2"/>
        </source>
    </evidence>
    <sequence length="654" mass="74762" checksum="51B4EA5692380319" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6H7</accession>
    <name>A0A6I8V6H7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Uncharacterized protein Usp1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">Usp1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736245.3">
        <property type="nucleotide sequence ID" value="XM_003736197.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038559.2">
        <property type="nucleotide sequence ID" value="XM_015183073.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232742.1">
        <property type="nucleotide sequence ID" value="XM_033376851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6H7"/>
    <dbReference type="KEGG" id="dpo:4800465"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V6H7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF905">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="340"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="536"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="629"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="849"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1077"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="583"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="629"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="854"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_003736245.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015038559.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033232742.1"/>
        </source>
    </evidence>
    <sequence length="1115" mass="119427" checksum="870CC195D404BBB4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7H0</accession>
    <name>A0A6I8V7H0_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Protein bunched, class 2/F/G isoform-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035442.2">
        <property type="nucleotide sequence ID" value="XM_015179956.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V7H0"/>
    <dbReference type="KEGG" id="dpo:26533499"/>
    <dbReference type="InParanoid" id="A0A6I8V7H0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026129661" evidence="1">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015035442.2"/>
        </source>
    </evidence>
    <sequence length="97" mass="10900" checksum="3B5B1031864157F0" modified="2020-08-12" version="1">MNLLHILIMALMAAAVASKHHRRHGPHHYHNHHNRHHFQQNVQNVHCGFGPYEETNLGGGGQYDGGLQAGDIKDTYDTTPDFDAYLSKIKNTSPSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBB2</accession>
    <name>A0A6I8VBB2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037664.2">
        <property type="nucleotide sequence ID" value="XM_015182178.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBB2"/>
    <dbReference type="KEGG" id="dpo:26532987"/>
    <dbReference type="InParanoid" id="A0A6I8VBB2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026235137" evidence="1">
        <location>
            <begin position="20"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015037664.2"/>
        </source>
    </evidence>
    <sequence length="168" mass="19128" checksum="49713FC180576291" modified="2020-08-12" version="1">MHPIIFLILATTIVVTTHTLTPNRTHYLDTMECGDCEKVNRECKIVSSGYYCREHDDSDKLDARQGVKANLRHLRKCMGPDHFFFNKEGISAACCFWTQEMGCQQVKNKMRTKPCSDCATYHERGSFLDLNPMGCPCGPQEKNGAENPLECFFFIIILMMCTILYGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHK1</accession>
    <name>A0A6I8VHK1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4815134</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_015041527.2">
        <property type="nucleotide sequence ID" value="XM_015186041.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VHK1"/>
    <dbReference type="FunCoup" id="A0A6I8VHK1">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VHK1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VHK1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000716">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004162">
        <property type="entry name" value="SINA-like_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45877:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45877">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SIAH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="213"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="13">
        <location>
            <begin position="265"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="59"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="609"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="557"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="617"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015041527.2"/>
        </source>
    </evidence>
    <sequence length="631" mass="69897" checksum="A7A73EAA8500152D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIV9</accession>
    <name>A0A6I8VIV9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12 13">ATP-binding cassette sub-family A member 7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">ldd</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12 13">MV-25-SWS-2005</strain>
            <tissue evidence="11 12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015042271.2">
        <property type="nucleotide sequence ID" value="XM_015186785.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042272.2">
        <property type="nucleotide sequence ID" value="XM_015186786.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042273.2">
        <property type="nucleotide sequence ID" value="XM_015186787.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VIV9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001253">
        <property type="entry name" value="ATP-binding cassette protein subfamily A, member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001574">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF241">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="642"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="683"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="716"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="748"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="821"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1232"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1407"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1448"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1485"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1515"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1604"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="865"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="1670"/>
            <end position="1888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="487"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1167"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1978"/>
            <end position="1998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="508"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015042271.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015042272.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015042273.2"/>
        </source>
    </evidence>
    <sequence length="2017" mass="225427" checksum="F25B169196E46C0E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPA7</accession>
    <name>A0A6I8VPA7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232609.1">
        <property type="nucleotide sequence ID" value="XM_033376718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPA7"/>
    <dbReference type="KEGG" id="dpo:117183299"/>
    <dbReference type="InParanoid" id="A0A6I8VPA7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232609.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28655" checksum="77E891625C57C98E" modified="2020-08-12" version="1">MTNKNTCKPEQKLQEFECERLCSAQIHNQLSMRVKITGESFHEYILQMKRIAARGTTDTESVIQYIFDGLNLKTDYKYTLNGCSSYKQLREKYEIYERTLAVDGGLAPKQKDSQPFGKKSNFTKYERKQHCYNCGSQENLRKDCRAALKCFRCNQTGHMSQDVSLLKMLVFGKMSGIHLQASSSTLGGLGNNITRSAGQFTAEVEIDGMRLAHKFLVVADHAVGFKLLLGHDFISKFTMTTTPGGYKFSPLPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPM9</accession>
    <name>A0A6I8VPM9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233030.1">
        <property type="nucleotide sequence ID" value="XM_033377139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPM9"/>
    <dbReference type="KEGG" id="dpo:117183386"/>
    <dbReference type="InParanoid" id="A0A6I8VPM9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233030.1"/>
        </source>
    </evidence>
    <sequence length="173" mass="20527" checksum="1A1B892526B26C0F" modified="2020-08-12" version="1">MRTTKINKSSFQDAVVFKFTNFVCESYNKSFFVFNTCRLKAINRNKVILNLNATILHTVNIFTVRFKLFKKANGFKPWMVDRSIDVCRFMHTSYDPYFKIIYELYRDFTNMNHTCPYVGHQILKGFYLRPELLRLPFPSGDYMLSLRWIFGKSESPTGDTNVSFSFVEDLRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPZ4</accession>
    <name>A0A6I8VPZ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6896912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233112.1">
        <property type="nucleotide sequence ID" value="XM_033377221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPZ4"/>
    <dbReference type="KEGG" id="dpo:6896912"/>
    <dbReference type="InParanoid" id="A0A6I8VPZ4"/>
    <dbReference type="OMA" id="KNPMMKY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026201400" evidence="1">
        <location>
            <begin position="19"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233112.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="22679" checksum="81D97549451D2989" modified="2020-08-12" version="1">MNLIQNALFLSLFQLIFSKENGSKSMWDEDDDSKKKPILRINHINVYGESGYMKVFTHIHTDRSHFDINVRLKRELGSNFLIFGLKVRVRPEGETIFVTLLQMKRLDLCGFFSDFSGNPMMRYFLKSKMHMSEIISCPVRVGNFSLLNISAYDIYPASLQNGTYKFFVEVVEGTGEIAKVFALQVTTDVIVPSQSAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VR84</accession>
    <name>A0A6I8VR84_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NSFL1 cofactor p47</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117183542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233575.1">
        <property type="nucleotide sequence ID" value="XM_033377684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VR84"/>
    <dbReference type="FunCoup" id="A0A6I8VR84">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117183542"/>
    <dbReference type="InParanoid" id="A0A6I8VR84"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VR84">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14273">
        <property type="entry name" value="UBA_TAP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01770">
        <property type="entry name" value="UBX_UBXN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF20">
        <property type="entry name" value="NSFL1 COFACTOR P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEP" evidence="3">
        <location>
            <begin position="147"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="251"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033233575.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="38467" checksum="18A7074B000EE0C3" modified="2020-08-12" version="1">MSDEQKLQSFMRKHDVQEDVARRYLVANDWALDQASGAYESDTAAVSSSPTDSSSNREPQSGIRLHTDRQDDDPEKHFAENSNNTIAPGEKRVSIESSTPALTYVDSLHSVRVWGQGRRLGSAHPINPPAPGSANQDSDTDPNDSEHTIVVLHLWSEGFSLDDGSLRLYDVPENAQFLQSVLQREEKMQGLGQRLEVSVQDHSNEPFRRLGQFLGPGRTLGDPLTRLANSSPSQSLARQEQHAEGKLSLNEKSAMTTVQLRLSDGSRISARFNRTHNVGDVYRYVRLARPQYSSRRFVLITSFPRVQLDETDARSLADANLCNVVVIQHLEEEQPEQSSSEFLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VS13</accession>
    <name>A0A6I8VS13_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">General transcription factor IIH subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16 17" type="primary">Tfb1</name>
    </gene>
    <organism evidence="13 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">MV-25-SWS-2005</strain>
            <strain evidence="13">MV2-25</strain>
            <tissue evidence="15 16">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="14 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 17">MV-25-SWS-2005</strain>
            <strain evidence="13">MV2-25</strain>
            <tissue evidence="14 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015039412.1">
        <property type="nucleotide sequence ID" value="XM_015183926.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039413.1">
        <property type="nucleotide sequence ID" value="XM_015183927.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039414.1">
        <property type="nucleotide sequence ID" value="XM_015183928.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233842.1">
        <property type="nucleotide sequence ID" value="XM_033377951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VS13"/>
    <dbReference type="SMR" id="A0A6I8VS13"/>
    <dbReference type="GeneID" id="4803891"/>
    <dbReference type="KEGG" id="dpo:4803891"/>
    <dbReference type="OMA" id="VCTCELL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080842">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000115">
        <property type="entry name" value="General transcription factor IIH subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="12">
        <location>
            <begin position="104"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="12">
        <location>
            <begin position="180"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015039412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015039413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015039414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033233842.1"/>
        </source>
    </evidence>
    <sequence length="587" mass="66517" checksum="7A271ABDACEE6E70" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSR6</accession>
    <name>A0A6I8VSR6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chitinase-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC117183602</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233953.1">
        <property type="nucleotide sequence ID" value="XM_033378062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VSR6"/>
    <dbReference type="FunCoup" id="A0A6I8VSR6">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117183602"/>
    <dbReference type="InParanoid" id="A0A6I8VSR6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026225306" evidence="5">
        <location>
            <begin position="21"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="7">
        <location>
            <begin position="23"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="389"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033233953.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="53180" checksum="0E57DBA5EA87480F" modified="2020-08-12" version="1">MWSLNCAFVLMIFMIYGCAAKDFKIFCQYDMGSFYYKNSSKFFSWNIDLRLCTHLVYGTGVGVDGETGDVTITDKYLLVENDILNVAHRLKRDNVKKVMFTIGGWLEESSDFSKMAADARKRDRFFTSLVRFMYGWGFDGVQIDWRYPTQRGGKPEDRKNFILLLEELKIICQEHQFILMVAVLGRTDKSTLEWYDIPKLVQYVHYINLMHHDNRDPYKMHLSYNAPLSGKNGVIESVKLWKKLSKAPGKLILNIPLFVRSYIMEKHKTTVGSPCKGPGLKTALTRKSGFMTYNEWCVQADTWTQKFDEQAQVPYAYRDDHWISFENNRSITEKLKLLKSQNLGGAISSTIDADDFSGKCGKKHALERVILETLDPQTVLVDMDAPTEPPPCLKDGVFRDINDCQLYYVCSAGHRSDYECRWQEFFDEAKSECRPMKEVKCEVLEAALKARELRDSTRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VU48</accession>
    <name>A0A6I8VU48_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234595.1">
        <property type="nucleotide sequence ID" value="XM_033378704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VU48"/>
    <dbReference type="KEGG" id="dpo:6898420"/>
    <dbReference type="InParanoid" id="A0A6I8VU48"/>
    <dbReference type="OMA" id="IFMDIRV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF1">
        <property type="entry name" value="MD-2-RELATED LIPID-RECOGNITION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033234595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16637" checksum="9201BFA9280BAA71" modified="2020-08-12" version="1">MKTTLLSTEFNYDGNYVEYFGLDPGKSKGVLGNLTKPMTKIFMDIRVVNEVMQRVVYKIDNFDGCAFLNNRVISRIFSRWYEAIVGNTTHFKCPVRPGIYYLENGATTGLVSQYLPAGTFHVSVRVKPIYGGPFAIEIIWRYMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W021</accession>
    <name>A0A6I8W021_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC117184216</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236677.1">
        <property type="nucleotide sequence ID" value="XM_033380786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W021"/>
    <dbReference type="KEGG" id="dpo:117184216"/>
    <dbReference type="InParanoid" id="A0A6I8W021"/>
    <dbReference type="OMA" id="EVYASSW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W021">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF13">
        <property type="entry name" value="SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="141"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033236677.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="34771" checksum="934405BD61BB6075" modified="2020-08-12" version="1">MKLHFEGHLDGVLDFSFSKVCLGDGGYYLASVAQDGRGFVRRGDTGEMCCNLVGHEGPVRAVSMGAKGTIVATGGDDGTARIWSVATGAELTMFRQDQKVSSVALDTRSEWMLTSSQERDSEVCLYDVTRGEQDKPLLCLYQKEGRAVRNVIFCRGDRSFLSSSYDRHIELWDILSGQRSHMISLPHHAKSMELCADGKTVTIAYGESIVLFDADTFNIIDHLKPPFKVTGASLHPQQQRFVCTSSYNEIYGCCSYTREFVKLHEAPELMGKQVNCIKYSPDGEVYASSWTNGQIILWQDYLNRKNRLMPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0D1</accession>
    <name>A0A6I8W0D1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="3 15">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 19 20" type="primary">rl</name>
    </gene>
    <organism evidence="17 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18 19 20">MV-25-SWS-2005</strain>
            <tissue evidence="18 19 20">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3 15"/>
    <dbReference type="RefSeq" id="XP_033236789.1">
        <property type="nucleotide sequence ID" value="XM_033380898.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236790.1">
        <property type="nucleotide sequence ID" value="XM_033380899.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236791.1">
        <property type="nucleotide sequence ID" value="XM_033380900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0D1"/>
    <dbReference type="KEGG" id="dpo:26533548"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W0D1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022412">
        <property type="term" value="P:cellular process involved in reproduction in multicellular organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002164">
        <property type="term" value="P:larval development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07849">
        <property type="entry name" value="STKc_ERK1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000181">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008349">
        <property type="entry name" value="MAPK_ERK1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01770">
        <property type="entry name" value="ERK1ERK2MAPK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8 15 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="38"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033236789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033236790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033236791.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="43116" checksum="60D4A18E020DB7C1" modified="2020-08-12" version="1">MAEANAAGTSVEGAVGLLAPHPNAELIRGQIFEVGPRYTKLAYIGEGAYGMVVSADDTITNQRVAIKKISPFEHQTYCQRTLREITILTRFKHENIIDIRDILRVDSIEQMRDVYIVQCLMETDLYKLLKTQRLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNKTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGVLGSPSREDLECIINEKARNYLESLPFKPNVPWSRLFPNADPLALDLLGKMLTFNPHKRIPVEEALAHPYLEQYYDPGDEPVAEVPFRINMENDDISRDALKSLIFEETLKFKERQPEQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0W3</accession>
    <name>A0A6I8W0W3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein Pvf2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Pvf2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236995.1">
        <property type="nucleotide sequence ID" value="XM_033381104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0W3"/>
    <dbReference type="FunCoup" id="A0A6I8W0W3">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817718"/>
    <dbReference type="InParanoid" id="A0A6I8W0W3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035099">
        <property type="term" value="P:hemocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21719">
        <property type="entry name" value="FI06402P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21719:SF1">
        <property type="entry name" value="FI06402P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026091960" evidence="2">
        <location>
            <begin position="17"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033236995.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="41659" checksum="88B6EAFD8551A5EA" modified="2020-08-12" version="1">MYLVFLLIFALALADGAKYQRSWLFNLPDNNYRDVRNVTARGVSFYLYNRPTVHPTSAPPPTSPTEPYDFYQEQDQDLDLDLDQEEDERVEGLEMGYHKRVPRHPHDNYNRVDGSSTDFDEDYPSDQAPAPGFLTAEKEKELGTPSRVQASRQAYEAYEKQEIKQRTIILLARAHNNRLIKEAECKVPKAQVIYMIGETNIQYYPRATILHRCDSSTGCCRDGEICSVKKNVTVELPFVLNNRGKMIPLVNHTECECVQNMSRRKRSTQCQCPKHFKDFSWRGSVVGAVMLHCRCDCHLNDAACQRLKNGEEGFAMNDRIRIQHRESSQPICNYGLYDVKNGRCPRPGSMHQLQTKLQWGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4K8</accession>
    <name>A0A6I8W4K8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein telomere ends associated-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6899970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033238004.1"/>
        </source>
    </evidence>
    <sequence length="1939" mass="217025" checksum="EE250493D9DD2270" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6C4</accession>
    <name>A0A6I8W6C4_DROPS</name>
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        <recommendedName>
            <fullName evidence="8">F-box/WD repeat-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">ago</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353051.4">
        <property type="nucleotide sequence ID" value="XM_001353015.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238910.1">
        <property type="nucleotide sequence ID" value="XM_033383019.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812667"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W6C4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010721">
        <property type="term" value="P:negative regulation of cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030162">
        <property type="term" value="P:regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22133">
        <property type="entry name" value="F-box_FBXW7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000004">
        <property type="entry name" value="F-box/WD repeat-containing protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000032">
        <property type="entry name" value="F-box/WD repeat-containing protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="11">
        <location>
            <begin position="985"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1100"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1127"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
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            <end position="1206"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1207"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
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            <begin position="1247"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1287"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1330"/>
            <end position="1369"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="140"/>
            <end position="208"/>
        </location>
    </feature>
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            <begin position="257"/>
            <end position="335"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="354"/>
            <end position="403"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="455"/>
            <end position="506"/>
        </location>
    </feature>
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            <begin position="573"/>
            <end position="673"/>
        </location>
    </feature>
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            <begin position="1037"/>
            <end position="1058"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="174"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="354"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <begin position="461"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="478"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
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        </location>
    </feature>
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    </feature>
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            <begin position="1041"/>
            <end position="1051"/>
        </location>
    </feature>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022574"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039915"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001353051.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033238910.1"/>
        </source>
    </evidence>
    <sequence length="1422" mass="150846" checksum="5A398AA61B3386F1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6S2</accession>
    <name>A0A6I8W6S2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">LOC117184639</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3">MV-25-SWS-2005</strain>
            <tissue evidence="2 3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239061.1">
        <property type="nucleotide sequence ID" value="XM_033383170.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239062.1">
        <property type="nucleotide sequence ID" value="XM_033383171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6S2"/>
    <dbReference type="KEGG" id="dpo:117184639"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033239061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239062.1"/>
        </source>
    </evidence>
    <sequence length="319" mass="35599" checksum="82839E55EC28F1E3" modified="2020-08-12" version="1">MAGIHTELTICRANSSTTMAALQHPRACVITATCFLRLIITGVFSTCIYSCRTTKRKIQTHLLAARSRVTPTKSIKSPQIELSGALLAVKLAKWILDQLRTATFYWSDATIVLFLINGDPHRWQTFVSNHVGQILEHSTSTQWRHVPTAVNPADCATRGLTPQELAVRPLWWSGPSWLQQPESQWPSNGVSSQDIDRTIIEEKPSVLFQNAVQVSEAPETVIQNSSSYDRLMSVMEYVLRFIHNIQAKGDKLSGPLSVQELDNVNSSRSQHSTGGLRDRNIEVEGKQGTIGPFLDDQQILRVRGRLGNAMHLPISQRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDQ7</accession>
    <name>A0A6I8WDQ7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117185439</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
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            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">MV-25-SWS-2005</strain>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
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    <sequence length="370" mass="41181" checksum="EC9F91B937B7C98B" modified="2020-08-12" version="1">MSDLVGTEEFSAAQLREWLESLNLPKGGSKAAMAARLNEIPVELRGQGPPAAETCENEREDEAAADQDSTKSERKEKNEKAPQAPGHNNNHGEYRAEVEMLKLQIELLKLQSEREKEGRGENTTPAANNDAGVMLLNAAKDMLPTYHGNISGNNDDVTTWIAQFKAVAKVNKLKDEKLLMLLMSKLKDKALVWLHSSLEHMSLPIDQLLNVMEDTFHPKESKLLLRRKFESRSWARGEEFSMYFNAKVSLASRIVIDDEEFIDGVIEGIPDVGLRRQAHMQCFGAPYQLLKAFEKIMLPKKYGSTEGANTGASPTPIRCYNCNSLGHVAGECRKPKRERGACYGCGSMSHQVSHCDEKKYKIASSTQGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
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            <taxon>Drosophila</taxon>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033240824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033240825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033240826.1"/>
        </source>
    </evidence>
    <sequence length="3310" mass="353297" checksum="D002AA6722756342" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8GBZ5</accession>
    <name>A0ABD8GBZ5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphotriesterase-related protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Parathion hydrolase-related protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4800604</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="5">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the metallo-dependent hydrolases superfamily. Phosphotriesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357849.1">
        <property type="nucleotide sequence ID" value="XM_001357812.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8GBZ5"/>
    <dbReference type="SMR" id="A0ABD8GBZ5"/>
    <dbReference type="GeneID" id="4800604"/>
    <dbReference type="KEGG" id="dpo:4800604"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00530">
        <property type="entry name" value="PTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017947">
        <property type="entry name" value="AryldialkylPase_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001559">
        <property type="entry name" value="Phosphotriesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10819">
        <property type="entry name" value="PHOSPHOTRIESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10819:SF3">
        <property type="entry name" value="PHOSPHOTRIESTERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02126">
        <property type="entry name" value="PTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01322">
        <property type="entry name" value="PHOSPHOTRIESTERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51347">
        <property type="entry name" value="PHOSPHOTRIESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601559-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001357849.1"/>
        </source>
    </evidence>
    <sequence length="350" mass="39490" checksum="49359AA20CB21248" modified="2025-06-18" version="1">MTAVETVLGSITPNLLGRTLTHEHVALDFEHFYRPPPADFESELKAKISMSTLGYVRLYPYSSKENVRFYDEEALEAAKKDVLLYKKHGGGSIVENSSYGLKRNLEFIVELAKSTGVHFIAGTGHYIHAVQDASHASLTVEQMSDLYTKDILTGIEIKGKMVKCGFIGEVASVYPIHEFEKNSIKATGEIQEVLGCGVSFHPHRDAQAPFDIMRLYLEAGGRAQKCVMSHLDRTLFKIEELVELSELGCYLQYDLFGTECSYYQLNTNVDMISDGQRIENLMKLIEEGLLDRLLMSHDIHTKHRLTSYGGHGYHHIHTNILPRMFARGVTLEQVEQMTVTNPANWLSFDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>B5DIP8</accession>
    <accession>A0A6I8UXJ7</accession>
    <name>B5DIP8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6903069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132788.1">
        <property type="nucleotide sequence ID" value="XM_002132752.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DIP8"/>
    <dbReference type="FunCoup" id="B5DIP8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="6903069"/>
    <dbReference type="KEGG" id="dpo:6903069"/>
    <dbReference type="eggNOG" id="ENOG502T8Q2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2485647_0_0_1"/>
    <dbReference type="InParanoid" id="B5DIP8"/>
    <dbReference type="OMA" id="EYIEMRS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247059">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DIP8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002132788.1"/>
        </source>
    </evidence>
    <sequence length="83" mass="9706" checksum="415A030955CDE721" modified="2008-10-14" version="1">MEISKKSKKSKNAKKDSTLTLKLTALQKKKKEVARVLTLKHEILFKSSVSYLEYLELRAEIERLNGLKDNFTRRVDKLKQQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>B5DP64</accession>
    <accession>A0A6I8V156</accession>
    <name>B5DP64_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl-coenzyme A thioesterase 13-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6900993</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134689.1">
        <property type="nucleotide sequence ID" value="XM_002134653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DP64"/>
    <dbReference type="STRING" id="46245.B5DP64"/>
    <dbReference type="GeneID" id="6900993"/>
    <dbReference type="KEGG" id="dpo:6900993"/>
    <dbReference type="eggNOG" id="KOG3328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_12_2_1"/>
    <dbReference type="InParanoid" id="B5DP64"/>
    <dbReference type="OMA" id="TIHGGFT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245740">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DP64">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000033">
        <property type="entry name" value="acyl-coenzyme A thioesterase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="54"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002134689.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="16110" checksum="6076672EE3A7CD51" modified="2008-10-14" version="1">MAGKRGLDIILHIAEYAPSKGGFDRVLKMIKITGGGEGRAFGEFTVEKEHLNQQGTLHSGLTGTIVDNISTYAMMSTGSHPGVTANLNVCYLSGAKAGEVVQIDARTVRAGRKMAYLDIFLKRKSDGILIATGRQIKYIDFDKKKLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPM2</accession>
    <accession>A0A6I8V0N7</accession>
    <name>B5DPM2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Divergent protein kinase domain 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aln</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIPK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134992.1">
        <property type="nucleotide sequence ID" value="XM_002134956.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPM2"/>
    <dbReference type="FunCoup" id="B5DPM2">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="GeneID" id="6900619"/>
    <dbReference type="KEGG" id="dpo:6900619"/>
    <dbReference type="eggNOG" id="ENOG502QSPC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042490_0_0_1"/>
    <dbReference type="InParanoid" id="B5DPM2"/>
    <dbReference type="OMA" id="KEHQVPG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245195">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DPM2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029244">
        <property type="entry name" value="FAM69_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21093:SF2">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21093">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14875">
        <property type="entry name" value="PIP49_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01299">
        <property type="entry name" value="PIP49_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAM69 N-terminal" evidence="9">
        <location>
            <begin position="9"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002134992.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="47495" checksum="08CBE3CBE9D3A810" modified="2008-10-14" version="1">MLSALRRKLRFGMQRTVLIAVLLAVGLFSYAFLNLKFCFKLMSHRSLNLMCQKFEQQEYSGSLCEELCGAQSTFDNFQCPLNDMKTILFTAEKNGDMYAVKLARHNDDELSWKNTKGESIYPKIEEFHEIVKLHIILAYNVTLDDNMIRALVNQEIADDNSQQMVSFWRLFKDNNYMMGKLFDEESVFPAVLGSCGPYYATEGLEIVQSNPSIMQYLASNRQQRLKHALNIMEYIFRMDEMKPEPLKMCKMQVNRFGTTPERRLKYQSAEHVYVESQLDKRLSRGVKCQSDKDCHFHSCRGMCNEERHACTHIQQNNNFQIFCEHILLGGGTFQPGLLSGVRLTRPMQKVVKMCVQPPKEHQVPGRHQAPNMQLAVRLYNDLKQLYQAAAAAAGNEAGEDGPGPRQIEDQRSD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q28XQ5</accession>
    <name>ASPG2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>L-asparaginase-like protein GA18140</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA18140</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ntn-hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL26261.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q28XQ5"/>
    <dbReference type="SMR" id="Q28XQ5"/>
    <dbReference type="FunCoup" id="Q28XQ5">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q28XQ5"/>
    <dbReference type="MEROPS" id="T02.001"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0277603">
        <property type="protein sequence ID" value="FBpp0276041"/>
        <property type="gene ID" value="FBgn0078148"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805250"/>
    <dbReference type="eggNOG" id="KOG1593">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021603_0_0_1"/>
    <dbReference type="InParanoid" id="Q28XQ5"/>
    <dbReference type="OMA" id="QAVIQGC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078148">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003948">
        <property type="term" value="F:N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04513">
        <property type="entry name" value="Glycosylasparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF6">
        <property type="entry name" value="N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000384145" description="L-asparaginase-like protein GA18140">
        <location>
            <begin position="21"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="72"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P20933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="EAL26261.2"/>
        </source>
    </evidence>
    <sequence length="457" mass="49694" checksum="83020CE4BA93509F" modified="2008-10-14" version="2" precursor="true">MRYLCRAQLLSLLLLPLLKARLSVPAHPIPKIFLPLMISTWNYTDANREAWTVLRQGPRRTRQAVIQGCLHCQNSNSCGRLLGGHSAPDSSGGITLEAALIDGANMDYGAVAGMAGIRNAIGVAHDVLRYTNHSLLVGEAAARFAEAMGHKKEVHSLSTTLDVLLPWLLGKCQPNFWRNVRPLANDSCGTFSPLPQEQHQREMRQEYPIEPGHHDQVGFLALDTEGHLHAASLSSGARFRIPGRVGDAAVPGAGIYADNQVGGALATGDGDVLMRFLPALLAVEALRAGQSPASAAEAVMRRLLRHHTEFNGGLVVVSRGGVYSAACAGLDEFQFVVSGESSGRSMRRVEGIKCLDRHEVVTGGPRGNFYVVPKKIWAAGGEDVLVQRVEKITLNEGVDSREFEEEYVEDKAAEIDVDEGEKAEEVRSLLDESPGDLLLHQLGLAPPFPFRFPFIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FL7</accession>
    <accession>A0A6I8UEX9</accession>
    <name>Q29FL7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger C4H2 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4814204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354330.2">
        <property type="nucleotide sequence ID" value="XM_001354294.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FL7"/>
    <dbReference type="SMR" id="Q29FL7"/>
    <dbReference type="FunCoup" id="Q29FL7">
        <property type="interactions" value="1331"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FL7"/>
    <dbReference type="GeneID" id="4814204"/>
    <dbReference type="KEGG" id="dpo:4814204"/>
    <dbReference type="eggNOG" id="KOG4451">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067420_0_0_1"/>
    <dbReference type="InParanoid" id="Q29FL7"/>
    <dbReference type="OMA" id="INMANRI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072016">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FL7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044069">
        <property type="entry name" value="ZF_C4H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018482">
        <property type="entry name" value="Znf-C4H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31058">
        <property type="entry name" value="ZINC FINGER C4H2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31058:SF2">
        <property type="entry name" value="ZINC FINGER C4H2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10146">
        <property type="entry name" value="zf-C4H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51896">
        <property type="entry name" value="ZF_C4H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354330.2"/>
        </source>
    </evidence>
    <sequence length="326" mass="35414" checksum="9A9B945647EE16CF" modified="2008-10-14" version="2">MATSEISSSNERHYYAKLEAIKDIRDKTLALEKLKVRIIKEVKLSDDEEKCLEEYRKEMEHLLEEKMSHVEELRQIHADINDMENIIKQTKENQTRSFDMANRVYEEYLALKYQIDHMRRDYLGLSPLRDLHEEEGSPISKDRFQTNFLKVAAQSAAAAAAAAAASVTQPTSLARPHARHPLMPEATVTALPPSSSSGGGGSSSVSAGGGGGGPGHAFMPPAPPGAGSAGAAAAAAAAAVRLGKGDFSAPPPPPPPTNRLQQTPSIGIGHHPSFRSDFNVNLRQQPPPMKSCLSCHQQIHRNAPICPLCKAKSRSRNPKKPKKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GN8</accession>
    <accession>A0A6I8UG92</accession>
    <name>Q29GN8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4814763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355015.1">
        <property type="nucleotide sequence ID" value="XM_001354979.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GN8"/>
    <dbReference type="FunCoup" id="Q29GN8">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="GeneID" id="4814763"/>
    <dbReference type="KEGG" id="dpo:4814763"/>
    <dbReference type="eggNOG" id="ENOG502TDEZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_597540_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GN8"/>
    <dbReference type="OMA" id="FFKYAPS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080739">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29GN8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355015.1"/>
        </source>
    </evidence>
    <sequence length="480" mass="53823" checksum="FDF909444745C824" modified="2006-04-04" version="1">MTNVWGKLLLKFFKYAPSCVVCLSFLPCGLIFGYTICSLVAERNLQAGLIYYGPLILGACWALVTALTMLTIKYVKKCIVYWQAWLLLAAGVLNLVASCFYFADGFDHVGAYISYLAHSMTFIAGYSFLHTISSKSSRSLMMSASCSCYLLGIATAVSLIGSAYSRNLKAFAGQTATTEQLIDGIYVNFAGTYLSIAAATLAILVGIKVLHFVGTVDLVNSMDNDLRIANGNGSIFEPRSEVIKRLQFFYVTKNQQWNVMLLLLFTEAIQFALFVYFIYWFALNPAIRLTQLADIDAMFWVIFGGSCLTLICLFFISVKVNFVASQMTLVFFTLLAMIICSSTDSRWSLWVLLVLFGMSYSSLQIALLEVTNLRFTECIICISYILKLICTSIVYYYFVADTKNSYFYATDKSTLMAQGFVFMIISSLLALVVGMKVPRTHRTKLMDIQYEVYGIIFMKHQIEQLNVKWLNDGTIPTISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29H06</accession>
    <accession>A0A6I8UFZ8</accession>
    <name>Q29H06_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chloride intracellular channel exc-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Clic</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chloride channel CLIC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354897.2">
        <property type="nucleotide sequence ID" value="XM_001354861.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29H06"/>
    <dbReference type="SMR" id="Q29H06"/>
    <dbReference type="FunCoup" id="Q29H06">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29H06"/>
    <dbReference type="GeneID" id="4814744"/>
    <dbReference type="KEGG" id="dpo:4814744"/>
    <dbReference type="eggNOG" id="KOG1422">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061051_0_0_1"/>
    <dbReference type="InParanoid" id="Q29H06"/>
    <dbReference type="OMA" id="IHHYKEQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070753">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29H06">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03198">
        <property type="entry name" value="GST_C_CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03061">
        <property type="entry name" value="GST_N_CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000040">
        <property type="entry name" value="chloride intracellular channel exc-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000188">
        <property type="entry name" value="Chloride intracellular channel exc-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053823">
        <property type="entry name" value="CLIC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43920:SF5">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43920">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22441">
        <property type="entry name" value="CLIC-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="CLIC N-terminal" evidence="6">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001354897.2"/>
        </source>
    </evidence>
    <sequence length="263" mass="30322" checksum="3B83F50BED22C107" modified="2008-10-14" version="2">MSAADVETQSQQETTNGSSKFDVPEIELIIKASTIDGRRKGACLFCQEYFMDLYLLAELKTISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKNIPGGYNLFVQDKEVATLIENLYVKLKLMLVKKDDAKNNALLSHLKKINDHLANRNTRFLTGDTMCCFDCELMPRLQHIRVAGKYFVDFEIPTHLTALWRYMYHMYQLDAFTQSCPADQDIINHYKLQQSLKMKKHEELETPTFTTSIPIDISE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IK4</accession>
    <name>RM22_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein uL22m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>39S ribosomal protein L22, mitochondrial</fullName>
            <shortName>L22mt</shortName>
            <shortName>MRP-L22</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mRpL22</name>
        <name type="ORF">GA18397</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379063">
        <property type="protein sequence ID" value="EAL32649.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001355590.2">
        <property type="nucleotide sequence ID" value="XM_001355554.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IK4"/>
    <dbReference type="SMR" id="Q29IK4"/>
    <dbReference type="FunCoup" id="Q29IK4">
        <property type="interactions" value="1419"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IK4"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0274304">
        <property type="protein sequence ID" value="FBpp0272742"/>
        <property type="gene ID" value="FBgn0078399"/>
    </dbReference>
    <dbReference type="GeneID" id="4815928"/>
    <dbReference type="KEGG" id="dpo:4815928"/>
    <dbReference type="CTD" id="29093"/>
    <dbReference type="eggNOG" id="KOG1711">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100005_0_0_1"/>
    <dbReference type="InParanoid" id="Q29IK4"/>
    <dbReference type="OMA" id="HKVIRQM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078399">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000009">
        <property type="entry name" value="39S ribosomal protein L22, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047867">
        <property type="entry name" value="Ribosomal_uL22_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="CHLOROPLAST 50S RIBOSOMAL PROTEIN L22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL22M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end status="unknown"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000323421" description="Large ribosomal subunit protein uL22m">
        <location>
            <begin status="unknown"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9NWU5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="233" mass="26692" checksum="1E54897AF317B81A" modified="2009-02-10" version="2" precursor="true">MHTVIRQMSQLRLQAMPSSALLKATASASAPSALDHSGAAVNHHSLHTAAGSGGALCASWNKHNNGPRKWLVYNKTVHPPQQPDEEPRKAYVCHMRSNIKYSPDKMWYIAAFVRGMSVDEALKQLNFVLKKGATDVKETILEAQEMAVQRHNVEYKSNLWVAESFVGKGRVFKGMRRHARGRFGQVEYKHCHYFVRLEEGQPPEHYYQEPQTPEQQYEHWLEQMRSRKVINSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JE8</accession>
    <accession>A0A6I8UH36</accession>
    <name>Q29JE8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein misato</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mst</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the misato family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355296.1">
        <property type="nucleotide sequence ID" value="XM_001355260.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JE8"/>
    <dbReference type="FunCoup" id="Q29JE8">
        <property type="interactions" value="1222"/>
    </dbReference>
    <dbReference type="GeneID" id="4816082"/>
    <dbReference type="KEGG" id="dpo:4816082"/>
    <dbReference type="HOGENOM" id="CLU_022511_1_0_1"/>
    <dbReference type="InParanoid" id="Q29JE8"/>
    <dbReference type="OMA" id="RMAAYGC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072897">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JE8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06060">
        <property type="entry name" value="misato"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="4">
        <location>
            <begin position="5"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="5">
        <location>
            <begin position="155"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001355296.1"/>
        </source>
    </evidence>
    <sequence length="574" mass="64883" checksum="FF23BD96C97EC022" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q29K75</accession>
    <accession>A0A6I8UIZ7</accession>
    <name>Q29K75_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">Arpc1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356240.1">
        <property type="nucleotide sequence ID" value="XM_001356204.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036812.1">
        <property type="nucleotide sequence ID" value="XM_015181326.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29K75"/>
    <dbReference type="SMR" id="Q29K75"/>
    <dbReference type="STRING" id="46245.Q29K75"/>
    <dbReference type="GeneID" id="4816622"/>
    <dbReference type="KEGG" id="dpo:4816622"/>
    <dbReference type="eggNOG" id="KOG1523">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034396_1_0_1"/>
    <dbReference type="OMA" id="YVWEPSP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081436">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29K75">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000030">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF2">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="51"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001356240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015036812.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="41669" checksum="38807BC29B5E6D78" modified="2006-04-04" version="1">MAETFTFGTSLASITCHAWNKDRTQIALSPNNHEIHIYSRDGNDWKLADVLNQHDLRVMGIDWAKNTNRIVSCAADRNAYVWTQGEDGKWKPTLVLLRINRAATCVKWSPAENKFAVGSGARLISVCYFESENDWWVSKHIKKPIRSTVTSLDWHPNNVLLVAGSTDYKVRVFSAFIKDVEEAPTPTPWGNRKPLGQLMAEFKNSLTSGGGWINNVSFSSDGNKVCWVGHDSCVSIADATNGNTVIRLRTGYLPFLSCEWVSPNSVVVAGYSCVPLLYSLTADNKLVLSGKLDKSQKRESSGITAMRIFQSMDRNMRTENTDTVVDSIHQNAITSVRIYAGDKSSASKVSTSGVDGQLVIWTVEQGGINGGMRNLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KQ8</accession>
    <accession>A0A6I8UIL6</accession>
    <name>Q29KQ8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication termination factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Replication termination factor 2 domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4816694</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the rtf2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356057.1">
        <property type="nucleotide sequence ID" value="XM_001356021.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KQ8"/>
    <dbReference type="SMR" id="Q29KQ8"/>
    <dbReference type="FunCoup" id="Q29KQ8">
        <property type="interactions" value="2595"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KQ8"/>
    <dbReference type="GeneID" id="4816694"/>
    <dbReference type="KEGG" id="dpo:4816694"/>
    <dbReference type="eggNOG" id="KOG3113">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048955_1_1_1"/>
    <dbReference type="InParanoid" id="Q29KQ8"/>
    <dbReference type="OMA" id="EFRWLHC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079593">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16653">
        <property type="entry name" value="RING-like_Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006735">
        <property type="entry name" value="Rtf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027799">
        <property type="entry name" value="Rtf2_RING-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775">
        <property type="entry name" value="PROTEIN C20ORF43 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775:SF0">
        <property type="entry name" value="REPLICATION TERMINATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04641">
        <property type="entry name" value="Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356057.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="33570" checksum="92FF5E65A45253F7" modified="2006-04-04" version="1">MGCDGGTIPKRDELVRVKQKPEQKDKDAEREFRWLHCSLTQQRLQEPIAMCGMGRLYSKQSLIERLLEKEKMPEMSEHVKSLKDIKTLQLTPNPAFTEEDKTEGLLDTRHAPYICKLIGLEMSGKFRFVALWSCGCVVSERALKQIKGNSSSTCPLCQTPYSVEDIVVLNGNEEDLELMKVKMEMRAAKRKSSKKDKKEAKKVEIKTETDESCASTVVTEKPSTSTSSKVKVVANPKRLGTANAMQDPELKRLKNDFSVAKDPKASDVYKSLFTSHKTEKEQERAHWITYNPFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MM0</accession>
    <accession>A0A6I8VXG9</accession>
    <name>Q29MM0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Cytidine deaminase</fullName>
            <ecNumber evidence="4 12">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 12">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4817054</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2'-deoxycytidine + H2O + H(+) = 2'-deoxyuridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13433"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>cytidine + H2O + H(+) = uridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="4 12"/>
    <dbReference type="RefSeq" id="XP_033235668.1">
        <property type="nucleotide sequence ID" value="XM_033379777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MM0"/>
    <dbReference type="FunCoup" id="Q29MM0">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MM0"/>
    <dbReference type="KEGG" id="dpo:4817054"/>
    <dbReference type="eggNOG" id="KOG0833">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097262_2_0_1"/>
    <dbReference type="InParanoid" id="Q29MM0"/>
    <dbReference type="OMA" id="QQVFTGC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081001">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055086">
        <property type="term" value="P:nucleobase-containing small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072527">
        <property type="term" value="P:pyrimidine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000008">
        <property type="entry name" value="Cytidine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050202">
        <property type="entry name" value="Cyt/Deoxycyt_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004064">
        <property type="entry name" value="PRK05578.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644:SF2">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11 12" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033235668.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="18638" checksum="46B90AC6426E3EB2" modified="2006-04-04" version="1">MAHLMENFARPEKTEEVVTYGSLDPSVQELLLAASEVRQRAYVPYSGFKVGSAFRATATNQIFTGCNVENAAFTPCSCAERTALAKAVSEGATEFSVGAVLAYEPDAFTTPCGVCRQFIREFATSDIPIYIAQAIDERIANKEEPLRSDDPVLCTSIFNLLPSSFRTYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q29N14</accession>
    <accession>A0A6I8UJQ1</accession>
    <name>Q29N14_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adult cuticle protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4816919</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356465.1">
        <property type="nucleotide sequence ID" value="XM_001356429.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29N14"/>
    <dbReference type="STRING" id="46245.Q29N14"/>
    <dbReference type="GeneID" id="4816919"/>
    <dbReference type="KEGG" id="dpo:4816919"/>
    <dbReference type="eggNOG" id="ENOG502T7IR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148241_0_0_1"/>
    <dbReference type="InParanoid" id="Q29N14"/>
    <dbReference type="OMA" id="AWNNGWA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080180">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031874">
        <property type="entry name" value="Cuticle_Acp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12336">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12336:SF0">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15955">
        <property type="entry name" value="Cuticle_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356465.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="14392" checksum="5C9408F68D81C087" modified="2006-04-04" version="1">MKFAAAVVMLVCALIGAEASWNALQIAPGVSYVNSVAHGAGAWGGPWNGAWNGGWNGAWNGGWNGAWGAPAAVAVHAGAGAPWGLTGPGWHGASVPGIALAQGPGLAAGHGHEGVYVAKTRGAIHTAPLAGHINSATSVNVAPAPGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q29N87</accession>
    <accession>A0A6I8V804</accession>
    <name>Q29N87_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inactive pancreatic lipase-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4816788</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035777.1">
        <property type="nucleotide sequence ID" value="XM_015180291.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29N87"/>
    <dbReference type="SMR" id="Q29N87"/>
    <dbReference type="FunCoup" id="Q29N87">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29N87"/>
    <dbReference type="ESTHER" id="drops-q29n87">
        <property type="family name" value="Insect_lipase"/>
    </dbReference>
    <dbReference type="GeneID" id="4816788"/>
    <dbReference type="KEGG" id="dpo:4816788"/>
    <dbReference type="eggNOG" id="ENOG502S0GJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027171_2_1_1"/>
    <dbReference type="InParanoid" id="Q29N87"/>
    <dbReference type="OMA" id="NCPTNIH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074472">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29N87">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000122">
        <property type="entry name" value="Vitellogenin-3-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF37">
        <property type="entry name" value="GH01208P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015035777.1"/>
        </source>
    </evidence>
    <sequence length="342" mass="38585" checksum="77F9CCFA094114B0" modified="2008-10-14" version="2">MHKAIKNKSRLLCGLKNAKADLTTAKFILYYGPTVADSDIYDLKDHKSLLEDENLDLSRNTVLYLHGYLEDPDVESIHVIAEAYLERKDTNLIVLDWGELADGNYIFDAMVNAKQLAPELAKVLLEMFDHGLDIEKLHIVGHSMGGQLAGNIGREIYKRTKGVRKIKRISALDPAFPLYYPLGAHLTSNDAEFVDVIHTDAWLYGAPTSTGTADFWPNGGNSLQPGCPKRNYKMLSDNDLSSHRRSWWFWAESVSDRFPIRFDAVPCRSWSDFKQNKTVETCPPVVMGHHCPTSIQGNFYLQTNGQTPFARGKEGTIYVDPKELLENTHGYSCEPRHSIKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M065</accession>
    <accession>A0A6I8UDA5</accession>
    <name>Q2M065_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Larval cuticle protein A2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Cpr62Bb</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353558.1">
        <property type="nucleotide sequence ID" value="XM_001353522.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M065"/>
    <dbReference type="FunCoup" id="Q2M065">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M065"/>
    <dbReference type="GeneID" id="4813110"/>
    <dbReference type="KEGG" id="dpo:4813110"/>
    <dbReference type="eggNOG" id="ENOG502S4VI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075165_5_1_1"/>
    <dbReference type="InParanoid" id="Q2M065"/>
    <dbReference type="OMA" id="FRRHANY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072685">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051217">
        <property type="entry name" value="Insect_Cuticle_Struc_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236:SF75">
        <property type="entry name" value="CUTICULAR PROTEIN 62BB, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12236">
        <property type="entry name" value="STRUCTURAL CONTITUENT OF CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353558.1"/>
        </source>
    </evidence>
    <sequence length="195" mass="21273" checksum="0C7F5EA096EEEF2C" modified="2006-02-21" version="1">MANILCFVILSLALFASVAVARPGYALDYYDHPKYAFNYGVADHTTGDVKSQHETRDGDVVKGQYSLVEPDGSIRTVDYTADSIHGFNAVVTKSGPTVHAQALVAKPIVAHKPILSHYEPQPHYVNHVAPVAAAPLVVAASPAPYVSKHYAPAPAAPIHYDYDDGYYNQGQQYEYIPQYDSGNYGHYASPYAGHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0G3</accession>
    <accession>A0A6I8UCW5</accession>
    <name>Q2M0G3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL50</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">39S ribosomal protein L50, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mRpL50</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL50 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353460.1">
        <property type="nucleotide sequence ID" value="XM_001353424.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0G3"/>
    <dbReference type="FunCoup" id="Q2M0G3">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0G3"/>
    <dbReference type="GeneID" id="4812821"/>
    <dbReference type="KEGG" id="dpo:4812821"/>
    <dbReference type="eggNOG" id="ENOG502S4J8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099202_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M0G3"/>
    <dbReference type="OMA" id="HIQYEYV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081195">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018305">
        <property type="entry name" value="Ribosomal_m50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31542">
        <property type="entry name" value="39A RIBOSOMAL PROTEIN L50, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31542:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10501">
        <property type="entry name" value="Ribosomal_L50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353460.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="21176" checksum="12FE6A4708FEE533" modified="2006-02-21" version="1">MAANLRKSQNLVGNLHKCFATAANVKKSAPIKSKTLAAVGESIAAKGFLRPHKPYAPPSNAADRVRSVASSLQLTSDNRKLDNLSEKFQFLSACFQELQHGVPNSQVHEICTVADVIAFYETPVDTTAPLDALRRTDLPENLHIQYEYLRFNPETDTKFNGKTAFPKSSTLVTGLKYRGKYEGHEAKRSWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0P6</accession>
    <accession>A0A6I8UCM3</accession>
    <name>Q2M0P6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">tRNA (Cytosine(72)-C(5))-methyltransferase NSUN6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4813750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353377.2">
        <property type="nucleotide sequence ID" value="XM_001353341.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0P6"/>
    <dbReference type="SMR" id="Q2M0P6"/>
    <dbReference type="FunCoup" id="Q2M0P6">
        <property type="interactions" value="1356"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0P6"/>
    <dbReference type="GeneID" id="4813750"/>
    <dbReference type="KEGG" id="dpo:4813750"/>
    <dbReference type="eggNOG" id="KOG1122">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_1_0_1"/>
    <dbReference type="InParanoid" id="Q2M0P6"/>
    <dbReference type="OMA" id="YQGAMLY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070820">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0P6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21150">
        <property type="entry name" value="PUA_NSun6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF34">
        <property type="entry name" value="TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="144"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="237"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353377.2"/>
        </source>
    </evidence>
    <sequence length="438" mass="47707" checksum="A270349CC7ED9938" modified="2008-10-14" version="2">MFYPKTPFLSNSLLEAEILKVNSAAVSQLLEWLCRTPRVTTYRVNRLRTNVETHKAQLEEHLARRYPCGQQAPRIYGLADLPEVLCIAPLEAELALSVPDPTLKEVIVDTSCGAALLRGAHIYAPGVLAMESNTQLQECVNVYADLAGKCKRGMTTRYENSEKVYVGVGKVLMQRYQLYNDKDEAPTGIAVEMQSNVSGVPSLGDLSSADALLQNLPSIVCVRVLDPQPGERILDMCAAPGNKTTHIAELMGDQGCVVALDNSASRVRGMLGKLGNNYRSIQAHVFDSTKAVAASSDSSCPSAPPFPCASFDRILLDAPCSGLGNRPQLLCNIKQAKILKSYPNIQRRLLAQAVQLLRPGGILVYSTCTVTEAECESIVAWALRKYPELRLLDATPRYGGPGLPLPDLETAQSKLLQRFGPGNDLDTVGFFIAKFQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0W3</accession>
    <accession>A0A6I8UCQ6</accession>
    <name>Q2M0W3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable deoxycytidylate deaminase</fullName>
            <ecNumber evidence="8">3.5.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">dCMP deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4813850</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Supplies the nucleotide substrate for thymidylate synthetase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dCMP + H2O + H(+) = dUMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22924"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.5.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.12" evidence="8"/>
    <dbReference type="RefSeq" id="XP_001353310.3">
        <property type="nucleotide sequence ID" value="XM_001353274.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0W3"/>
    <dbReference type="FunCoup" id="Q2M0W3">
        <property type="interactions" value="756"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0W3"/>
    <dbReference type="GeneID" id="4813850"/>
    <dbReference type="KEGG" id="dpo:4813850"/>
    <dbReference type="eggNOG" id="KOG3127">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047993_1_2_1"/>
    <dbReference type="InParanoid" id="Q2M0W3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079974">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0W3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000021">
        <property type="entry name" value="Deoxycytidylate deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016473">
        <property type="entry name" value="dCMP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086:SF18">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="DEOXYCYTIDYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006019">
        <property type="entry name" value="dCMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001353310.3"/>
        </source>
    </evidence>
    <sequence length="187" mass="20951" checksum="30D10CA9EB9F3F3E" modified="2016-01-20" version="3">MSDASANKEVSEAGNSKRQDYLHWDDYFMATSLLSAQRSKDPVTQVGACIVDQQKRIVAIGYNGLPRNCSDDEFPWSKLSKNSSPDSLEEKKLYVVHAEANAILNSNGGSLVGTCLYTTLFPCNECAKLIIQSGIQKVFYMSDKYAHKKIYRASRRMLDSVGVVCTRHVPAQKQILIDFDAFLQDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M1A2</accession>
    <accession>A0A6I8UCE0</accession>
    <name>Q2M1A2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="4 13">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Pdi</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4 13"/>
    <dbReference type="RefSeq" id="XP_001353171.1">
        <property type="nucleotide sequence ID" value="XM_001353135.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M1A2"/>
    <dbReference type="FunCoup" id="Q2M1A2">
        <property type="interactions" value="1470"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M1A2"/>
    <dbReference type="GeneID" id="4813455"/>
    <dbReference type="KEGG" id="dpo:4813455"/>
    <dbReference type="eggNOG" id="KOG0190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025879_1_0_1"/>
    <dbReference type="InParanoid" id="Q2M1A2"/>
    <dbReference type="OMA" id="FFGMKKD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080005">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M1A2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000023">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000030">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000027">
        <property type="entry name" value="protein disulfide-isomerase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000042">
        <property type="entry name" value="protein disulfide-isomerase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF240">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035543882" description="Protein disulfide-isomerase" evidence="13">
        <location>
            <begin position="19"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="53"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="394"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001353171.1"/>
        </source>
    </evidence>
    <sequence length="493" mass="55372" checksum="ACF5EB6FFBE301B0" modified="2006-02-21" version="1">MKFLICTLLAAAFYVAAAEVKVEEGVLIATVDNFKQLVADNEFVLVEFYAPWCGHCKALAPEYAKAAQQLAEKDSPIKLAKVDATVEGELAEQYQVRGYPTLKFFRSGAPVEYSGGRQAADIISWVTKKTGPPAKDLTSVADAEQFLKDNEIAIIGFFKDTDSEEAKTFTKAANALDSFVFGVSSNADIIAKYEAKDNGVILFKPFDDKKSVFEGELTEETLKKFAQVQSLPLIVDFNHESASKIFGGSIKSHLLFFVSKEAGHIEAHVDPLKEIAKKYRDDILFVTISSDEEDHTRIFEFFGMNKEEVPTIRLIKLEEDMAKYKPESNDLSAETIEAFLKKFLDGKLKQHLLSQDLPEDWDKQPVKVLVSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEKYKDNTDIVIAKMDSTANELENIKISSFPTIKYFRKDDNKVIDYNLDRTLDDFIKFLDANGEVADAEPAEETEEEEEAPKKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAV6</accession>
    <name>A0A6I8UAV6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N6-L-threonylcarbamoyladenine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Tcs3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001352566.2">
        <property type="nucleotide sequence ID" value="XM_001352530.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAV6"/>
    <dbReference type="FunCoup" id="A0A6I8UAV6">
        <property type="interactions" value="716"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812455"/>
    <dbReference type="InParanoid" id="A0A6I8UAV6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAV6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24132">
        <property type="entry name" value="ASKHA_NBD_OSGEP_like_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000141">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000295">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="11">
        <location>
            <begin position="24"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="130"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001352566.2"/>
        </source>
    </evidence>
    <sequence length="347" mass="38053" checksum="12E9D008B7E584E4" modified="2020-08-12" version="1">MVCSLGIEGSANKIGVGIIRDGEVLANVRRTYITPPGEGFLPNATAKHHREVILALVQESLKEAKIKPSDLDVICYTKGPGMAPPLLVGAIVARTLSLLWEKPLLGVNHCIGHIEMGRHITGAQNPIILYVSGGNTQVIAYSNKKYRIFGETIDIAVGNCLDRFARILKLPNDPSPGYNIEQMAKEGTNYINLPYVVKGMDVSFSGILSHIEALADPTKNPNKRKKTLEADASVAKPADLCFSLQETIFAMLVEITERAMAHCGSNEVLIVGGVGCNERLQKMMGIMCEERGGKLFAIDERYCIDNGLMIAHAGAEMFKSGSRMPFEESFVTQRYRTDEVLVTWRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCV9</accession>
    <name>A0A6I8UCV9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/arginine repetitive matrix protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353485.3">
        <property type="nucleotide sequence ID" value="XM_001353449.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCV9"/>
    <dbReference type="FunCoup" id="A0A6I8UCV9">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813565"/>
    <dbReference type="InParanoid" id="A0A6I8UCV9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCV9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353485.3"/>
        </source>
    </evidence>
    <sequence length="348" mass="39289" checksum="69DB6046AA797EC9" modified="2020-08-12" version="1">MGDGDDLTSQAIAELIREIEGGDARSSSGVQERKVNPLGKPNKRFLGRTINTALRHNQRESERMQAECQRKLKDLDKRYEKRKSNYYYNRDAGRYRSRSSSRSRSRSRSRKVEKRRSSSRRRRSSSSRSPSYSRSRKSSHKKRKHQSKHKRKKKTKHKRRARSSSSRSSSPEEKPVPTVTELSSSELFFYQSRQVALAMAMSYGQGANGPQVNGRKIKERASSSLSDIARELMSDNEHGNVDSRLINSLSISSSENGQELLTIDVSSSSGKEEESSAESRGTEISNAENGAGCFIALDDSTTESESGHNSDIEIIEECQVEQNQEPERRQQQVDDSRSAIDSVDLTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDB9</accession>
    <name>A0A6I8UDB9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sarcoplasmic reticulum histidine-rich calcium-binding protein isoform X3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812883</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353644.3">
        <property type="nucleotide sequence ID" value="XM_001353608.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDB9"/>
    <dbReference type="KEGG" id="dpo:4812883"/>
    <dbReference type="InParanoid" id="A0A6I8UDB9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026314022" evidence="2">
        <location>
            <begin position="17"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="137"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="155"/>
            <end position="182"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="227"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353644.3"/>
        </source>
    </evidence>
    <sequence length="466" mass="51687" checksum="F098B41987F10474" modified="2020-08-12" version="1">MKSIVIFLALFAACHAAPTTPANVKEATEALSRPSPIAPDVISDPKPVGKVVLLKSEPTLHRQKRDETAHPKPDSVKARDLLQHNPLHQQESLGIQTHEPLKHEDKKSKREADHEEGHHEEGHHEEPKKEDAKDAHTHALHAAAPAVEAHKKSKREADHEEGHHEESHHEEPKKEDAKDAHTHALHAAAPAVEAHKKSKREADHEEGHHEEGHHEEPKKEDAKDAHTHALHAAAPAVEAHKKSKREADHEEGHHEEGHHEEPKKDEAKDNHAHADAHKQLKREAHHEEGHHEEGHHEEPKKDALPHALPASAHTAEAPKQKRNSPPKPEASKSEHRESHHREEQHSAKPDSHAHHKQRREAPSKPASETAHEGLQHDPKKAEELHKAIEALAAGHSKDAQRHQRDIPVPTEIKSSSAPANNKATTELAIPIHHPVSVAELFHKEKPAAAAGSSSEESKEKSKEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEX3</accession>
    <name>A0A6I8UEX3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="21 22 23">A disintegrin and metalloproteinase with thrombospondin motifs 7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21 22 23" type="primary">AdamTS-B</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21 22 23">MV-25-SWS-2005</strain>
            <tissue evidence="21 22 23">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354458.2">
        <property type="nucleotide sequence ID" value="XM_001354422.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042307.2">
        <property type="nucleotide sequence ID" value="XM_015186821.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239306.1">
        <property type="nucleotide sequence ID" value="XM_033383415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEX3"/>
    <dbReference type="KEGG" id="dpo:4814368"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000006">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF200">
        <property type="entry name" value="ADAM METALLOPEPTIDASE WITH THROMBOSPONDIN TYPE 1 MOTIF B, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 21 22" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="9 14 16" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634439" evidence="18">
        <location>
            <begin position="21"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="19">
        <location>
            <begin position="325"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="186"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="241"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="266"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="active site" evidence="13 16">
        <location>
            <position position="469"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="401"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="430"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="446"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="578"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="635"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="639"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="650"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001354458.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_015042307.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_033239306.1"/>
        </source>
    </evidence>
    <sequence length="1037" mass="116776" checksum="390FA33133B2CDA1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGB2</accession>
    <name>A0A6I8UGB2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Activating signal cointegrator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4814859</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355031.2">
        <property type="nucleotide sequence ID" value="XM_001354995.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGB2"/>
    <dbReference type="FunCoup" id="A0A6I8UGB2">
        <property type="interactions" value="1196"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814859"/>
    <dbReference type="InParanoid" id="A0A6I8UGB2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGB2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06554">
        <property type="entry name" value="ASCH_ASC-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.30:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056994">
        <property type="entry name" value="TRI4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056993">
        <property type="entry name" value="TRIP4_3rd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23135">
        <property type="entry name" value="TRI4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23134">
        <property type="entry name" value="TRIP4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="2">
        <location>
            <begin position="388"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355031.2"/>
        </source>
    </evidence>
    <sequence length="532" mass="59956" checksum="C42750AF084410E0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGP4</accession>
    <name>A0A6I8UGP4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Nek2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001355206.4">
        <property type="nucleotide sequence ID" value="XM_001355170.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGP4"/>
    <dbReference type="FunCoup" id="A0A6I8UGP4">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815337"/>
    <dbReference type="InParanoid" id="A0A6I8UGP4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08217">
        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF10">
        <property type="entry name" value="NIMA-RELATED KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="80"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="670"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="738"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="485"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="670"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="782"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355206.4"/>
        </source>
    </evidence>
    <sequence length="791" mass="88494" checksum="375F67CFE6E4B705" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGZ1</accession>
    <name>A0A6I8UGZ1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial potassium channel ATP-binding subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-binding cassette sub-family B member 8, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial sulfonylurea-receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC4815448</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">MV-25-SWS-2005</strain>
            <tissue evidence="23">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355372.3">
        <property type="nucleotide sequence ID" value="XM_001355336.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGZ1"/>
    <dbReference type="FunCoup" id="A0A6I8UGZ1">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815448"/>
    <dbReference type="InParanoid" id="A0A6I8UGZ1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGZ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18574">
        <property type="entry name" value="ABC_6TM_ABCB8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000195">
        <property type="entry name" value="AGAP006273-PA-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000403">
        <property type="entry name" value="ATP-binding cassette sub-family B member 8, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
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    <sequence length="743" mass="82470" checksum="6602A88D39B4D74B" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8UHY5</accession>
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            <strain evidence="10">MV-25-SWS-2005</strain>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UI77</accession>
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            <taxon>Diptera</taxon>
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        <property type="entry name" value="PLDc_mTdp1_1"/>
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    <sequence length="576" mass="63768" checksum="829C5AAE64870DF1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIH8</accession>
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        <name evidence="3" type="primary">LOC4816452</name>
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            <begin position="212"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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            <dbReference type="RefSeq" id="XP_001356019.3"/>
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            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="222" mass="24112" checksum="66F824E50B2EB359" modified="2020-08-12" version="1">MSEPGSSKDLPGAMEKAQVLRQEISRSSREISFNFWQEFERIRAVQMQRLLQERQERERISKLMVAANAEAHQLTEALSRSIVTQGVSLETLPQPAIANKEDSGSGNKTPAAPQPATDTKRPLHTGVGAVAAPSLLPQRLRAQNQRLQMANKPKAPPKPILKPAARPPASRGSPARVGSAPVSNRTPNRLSANTQKGAKLSGSKTTPDIAKSRLRKTSKNTH</sequence>
</entry>
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            <text>ATP + H2O = ADP + phosphate + H(+)</text>
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        <property type="term" value="P:germ-line stem cell division"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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            <begin position="20"/>
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        </location>
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            <begin position="995"/>
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            <begin position="333"/>
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        <location>
            <begin position="1204"/>
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        <location>
            <begin position="1447"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00047984"/>
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            <dbReference type="Pfam" id="PF00567"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_001356319.3"/>
        </source>
    </evidence>
    <sequence length="1466" mass="164060" checksum="B16618C3EC61980E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKU9</accession>
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        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
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        </recommendedName>
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        <name evidence="20" type="primary">Elp3</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_001356980.2">
        <property type="nucleotide sequence ID" value="XM_001356944.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKU9"/>
    <dbReference type="SMR" id="A0A6I8UKU9"/>
    <dbReference type="FunCoup" id="A0A6I8UKU9">
        <property type="interactions" value="2197"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817575"/>
    <dbReference type="InParanoid" id="A0A6I8UKU9"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKU9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="84"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="398"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001356980.2"/>
        </source>
    </evidence>
    <sequence length="550" mass="62397" checksum="1EE5E039D30EE74B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN34</accession>
    <name>A0A6I8UN34_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4800826</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001358030.1">
        <property type="nucleotide sequence ID" value="XM_001357993.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN34"/>
    <dbReference type="FunCoup" id="A0A6I8UN34">
        <property type="interactions" value="2328"/>
    </dbReference>
    <dbReference type="GeneID" id="4800826"/>
    <dbReference type="KEGG" id="dpo:4800826"/>
    <dbReference type="InParanoid" id="A0A6I8UN34"/>
    <dbReference type="OMA" id="WANHGYF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078728">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="88"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="393"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358030.1"/>
        </source>
    </evidence>
    <sequence length="426" mass="48928" checksum="B59A02FB1657733A" modified="2020-08-12" version="1">MSAEISGFRRFIHWGPITALSIIKCITLTTLYMNSMWWPPNESFAGFAHQALFLMLSTLATFNYVMATLTGPGLLPRQWQPKELKDTEYLQYCKACEGYKAPRSHHCRKCNRCVKKMDHHCPWINHCVGWANHAYFSYFLLFSILGSMQATVVLCGSFYRGIYRYYYLTHGLVHLASVQFTVYSIIMCITGMGLAIGVVIGLGMLLFIQLKTIVANQTGIEIWIVEKAIYRRYAIGESEDSFVYPYDLGWWLNLKQVFNDECQKLGDGIDWPVLKGCDQYTLTREQLAQKEEKRARTRTYKCHSPVTGRWLPVCSQGWSVCMGAPCTDEPRIRLQPGDIIKVTRFRKHWLFGERELTKQELGADKTHQRKGHIRGWFPRRSAVEVIEVHECGGEPGSDNLPENGTCNGGHAQLRQQQRNGHAKKYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNL1</accession>
    <name>A0A6I8UNL1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phospholipase A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4801131</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358273.2">
        <property type="nucleotide sequence ID" value="XM_001358236.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNL1"/>
    <dbReference type="FunCoup" id="A0A6I8UNL1">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="ESTHER" id="drops-q29ae0">
        <property type="family name" value="Insect_lipase"/>
    </dbReference>
    <dbReference type="GeneID" id="4801131"/>
    <dbReference type="KEGG" id="dpo:4801131"/>
    <dbReference type="InParanoid" id="A0A6I8UNL1"/>
    <dbReference type="OMA" id="CGNYESA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079491">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000076">
        <property type="entry name" value="phospholipase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002334">
        <property type="entry name" value="Allerg_PlipaseA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF150">
        <property type="entry name" value="FI01825P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00825">
        <property type="entry name" value="DOLALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026019439" evidence="5">
        <location>
            <begin position="19"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="59"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358273.2"/>
        </source>
    </evidence>
    <sequence length="339" mass="36841" checksum="9D305BA469CA1E9A" modified="2020-08-12" version="1">MIMRLLLALAFCLVAVNAVEVRENGENGWYVPQADGSFEWMDRDVAEAYLEAHAEKEARNVLNPVTFYLYTQSNRGWAQEIKASSSVISSSYFNPNNPTRFTIHGWSSSADEFINYGVRDAWFSHGDMNMIAVDWGRARSVDYASSVLAVPGVGEQVATLINFMRNNHGLNLDNTMVIGHSLGAHVSGYAGKNVKNGQLHTIIGLDPALPLFSYDSPNKRLSSTDAWYVESIQTNGGTLGFLKPIGKGAFYPNGGKSQPGCGLDLTGSCAHSRSVIYYAEAVTQDNFPTMRCGNYESAVSSSCGSSYSSVKMGAVVNAYMVSGDYYVPVRSDAPYGMGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNY0</accession>
    <name>A0A6I8UNY0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Beta-1,4-galactosyltransferase 7</fullName>
            <ecNumber evidence="16">2.4.1.133</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">beta4GalT7</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">MV-25-SWS-2005</strain>
            <tissue evidence="23">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3-O-(beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-galactose = 3-O-(beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15297"/>
            <dbReference type="Rhea" id="RHEA-COMP:12567"/>
            <dbReference type="Rhea" id="RHEA-COMP:12570"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:132085"/>
            <dbReference type="ChEBI" id="CHEBI:132088"/>
            <dbReference type="EC" id="2.4.1.133"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.133" evidence="16"/>
    <dbReference type="RefSeq" id="XP_001358478.1">
        <property type="nucleotide sequence ID" value="XM_001358441.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNY0"/>
    <dbReference type="FunCoup" id="A0A6I8UNY0">
        <property type="interactions" value="1432"/>
    </dbReference>
    <dbReference type="GeneID" id="4801371"/>
    <dbReference type="KEGG" id="dpo:4801371"/>
    <dbReference type="InParanoid" id="A0A6I8UNY0"/>
    <dbReference type="OMA" id="NWLFVCG"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071250">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046525">
        <property type="term" value="F:xylosylprotein 4-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000062">
        <property type="entry name" value="beta-1,4-galactosyltransferase 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF30">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="21">
        <location>
            <begin position="68"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="20">
        <location>
            <begin position="164"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_001358478.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="36573" checksum="265C443C84AC45AD" modified="2020-08-12" version="1">MVNISTINWVFVCGLSFCMGGIAVLSFMPLGSDCICPLSNPLAKLVGSGPVQKKSASVTPAPKEQDHVHSTAAHKMAVLVPFRDRFEELLQFVPHITDFLRRQGVAHHIFLLNQVDRFRFNRASLINVGFQFCHEVYDYIAMHDVDLLPRNDDLLYEYPSNMGPLHIAGPKLHPKYHYDNFVGGILLVRREHFQKMNGMSNQYWGWGLEDDEFFVRIRDAGLQVTRPQNIKTGINDTFGHIHNRHHRKRDTQKCFNQKEMTRKRDHKTGLDNVKYKILKVQGLTIEGIDITVLNIMLECDVNKTPWCDCSGTAAAASVVQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPH8</accession>
    <name>A0A6I8UPH8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358822.4">
        <property type="nucleotide sequence ID" value="XM_001358785.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPH8"/>
    <dbReference type="KEGG" id="dpo:4801785"/>
    <dbReference type="InParanoid" id="A0A6I8UPH8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026313634" evidence="2">
        <location>
            <begin position="20"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358822.4"/>
        </source>
    </evidence>
    <sequence length="137" mass="14771" checksum="498735D9BB9BA44C" modified="2020-08-12" version="1">MKFLVTIAALCLCLAYVTGQQYFLGKFPSRARFGFDPLSLAQPSSATQQVRDPRQNRGPVVFPPSPPDAVDESSGVVVGASGYGFVPPQQTAAIVAAAAAAAVADPTFFSTSFQTPDTSYATYNYFSNPYTTRFRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPL2</accession>
    <name>A0A6I8UPL2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein new-glue 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801722</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358767.4">
        <property type="nucleotide sequence ID" value="XM_001358730.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPL2"/>
    <dbReference type="KEGG" id="dpo:4801722"/>
    <dbReference type="InParanoid" id="A0A6I8UPL2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026240609" evidence="2">
        <location>
            <begin position="23"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358767.4"/>
        </source>
    </evidence>
    <sequence length="133" mass="13933" checksum="E943D77BD77B0CCB" modified="2020-08-12" version="1">MRTGQLAVGLLAGSLCIWLTNAATSTTATTSSGTSTTVAATTTTSAATTTTTTAATTAATTSAATTAASTGTVHRKKIRISNLKWSSKRRIKVHRGTTNRSTRSRSVLGRRILRSRNGSRARVLVLNQRRAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQV6</accession>
    <name>A0A6I8UQV6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription initiation protein SPT3 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Spt3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SPT3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359484.2">
        <property type="nucleotide sequence ID" value="XM_001359447.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQV6"/>
    <dbReference type="FunCoup" id="A0A6I8UQV6">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="GeneID" id="4802591"/>
    <dbReference type="KEGG" id="dpo:4802591"/>
    <dbReference type="InParanoid" id="A0A6I8UQV6"/>
    <dbReference type="OMA" id="QFMFNEQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076413">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07978">
        <property type="entry name" value="HFD_TAF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359484.2"/>
        </source>
    </evidence>
    <sequence length="386" mass="43912" checksum="0D5E9B7322DAD040" modified="2020-08-12" version="1">MNHNTKRVMRQNSVCILTNDLASSPVMLAPTPPSAPTTPQLQTVEDIKPIHVQRQVILLQSGNIAQTPAPPHGAAPQHANMAAPSAVNANSVGNPPVQDDAPVSLLTEIADIMRSFGDSDQPRTASVKLVEQILQQQLRGIFNEASLVAMRRKQNPCPSQADFEFLMRNHLVKIARMRKHLKDMRILKRFLSIRTGRQDFMDDLEQQEEDEELAIEVHELHDEDRMRRLFRADRISQILTGQQYLEFNEARKTSFYCRHGEKIKNKFRRFLDLPADLRIPTPTMNILAYLAHETIAAIVDYSILTRLNSDNRATEPYSRVTSAGGSPAMMHVCPEVTQGRGMEVVKPISVQEIHEAMRRFRQMSSRKIGRYRNSCDIDFRRSFLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UT98</accession>
    <name>A0A6I8UT98_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Venom serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4803585</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360289.3">
        <property type="nucleotide sequence ID" value="XM_001360252.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UT98"/>
    <dbReference type="SMR" id="A0A6I8UT98"/>
    <dbReference type="FunCoup" id="A0A6I8UT98">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803585"/>
    <dbReference type="InParanoid" id="A0A6I8UT98"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035008">
        <property type="term" value="P:positive regulation of melanization defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160032">
        <property type="term" value="P:Toll receptor ligand protein activation cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000015">
        <property type="entry name" value="Atrial natriuretic peptide-converting enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026361881" evidence="9">
        <location>
            <begin position="23"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="10">
        <location>
            <begin position="27"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="153"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001360289.3"/>
        </source>
    </evidence>
    <sequence length="402" mass="44115" checksum="5AF0739EB2467213" modified="2020-08-12" version="1">MFGRSLIITEVWLLALAPVALAYFEGCDNTYNLSPGTTYVESPYYPGSYPAGTSCRYRFVAPVDYSVQVQCTISLPQSSGQCSTDNFWLDPEGDLLMRGAENFCGSGTFARESLFTELVFAYISTGQQGGSFKCTLTTSRQNCNCGWSVNSRIANGQQAADNEFPSMVALKDVTSNQPSFCGGTIVAHRYILTAAHCTLQIKQATNIVAIAGTNNLQNPSNSRYYQQYAIQQMIRHEQYEDEPDVKNDIAVLITAQNILWSRGVGPICLPPVGTTQTFSYQTVDVIGWGTLFFAGPTSATMQKINLMVVNNQDCQTEYNDVAEIYPSQMCTYDYSGNARDSCQYDSGGPVILRSSKQFLLGIISFGKSCAANAYPMGVNTRITSHISWIRQKIGNTNCVVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTP7</accession>
    <name>A0A6I8UTP7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein TsetseEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4804114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360726.3">
        <property type="nucleotide sequence ID" value="XM_001360689.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTP7"/>
    <dbReference type="FunCoup" id="A0A6I8UTP7">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804114"/>
    <dbReference type="InParanoid" id="A0A6I8UTP7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007931">
        <property type="entry name" value="TsetseEP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05267">
        <property type="entry name" value="DUF725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026185937" description="Protein TsetseEP domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Protein TsetseEP" evidence="3">
        <location>
            <begin position="52"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360726.3"/>
        </source>
    </evidence>
    <sequence length="259" mass="28440" checksum="3453F52ED6B7F7FE" modified="2020-08-12" version="1">MQHGLLVVILLACLAASSSSTATPTSLMSGHSRMLQLMSSTSELQRDNPTRSMACFQYYSEEFDKHLKQFEYEYGLCKEQAINQTADLYAKYNQVVYSLGNTTIEVCKALTDCTHGANALESLNCYSAQGSESVRNAYNLSSTASLYSADLTQDLQQVTFREEGCSNSSSRRYEARLDQSYIDLQNCLSGASPVPVPVPTTTTTPPSTSVISTTEDSTTQEIIPTTWNYQADPKDDGSIASDKNHRLASKIGHIIKHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTQ7</accession>
    <name>A0A6I8UTQ7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Coq9</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the COQ9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360738.4">
        <property type="nucleotide sequence ID" value="XM_001360701.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTQ7"/>
    <dbReference type="FunCoup" id="A0A6I8UTQ7">
        <property type="interactions" value="1369"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804132"/>
    <dbReference type="InParanoid" id="A0A6I8UTQ7"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.10:FF:000004">
        <property type="entry name" value="Ubiquinone biosynthesis protein COQ9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.10">
        <property type="entry name" value="Tetracycline Repressor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048674">
        <property type="entry name" value="COQ9_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21392">
        <property type="entry name" value="COQ9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="13" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="4 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="Ubiquinone biosynthesis protein COQ9 HTH" evidence="11">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="COQ9 C-terminal" evidence="10">
        <location>
            <begin position="228"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="45"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001360738.4"/>
        </source>
    </evidence>
    <sequence length="333" mass="36769" checksum="6BFC81BC8FEBD493" modified="2020-08-12" version="1">MANVRCLQKLFRLQKLLLPLHAQTCHLASLHRQLPLAAASLARGYAKDATDKTTGSTSLEDFRAREEQLERERDAAEEAAAKATQNEAGSGGAGGAGGKQQESAEQSAKQAKVDAIRDKILDAALEHVPQHGWTRQAIVLGAEQCGYPSVVHGMFPEGGFALVSHFNGRCNAQLVQSLKEKTSKGQQEVENPLDFLVQAVRQRMEMIAPYKAQWPQAMSLIAQPQHASTALAQVLTLVDDICYYSGDRSVDFGWYTRRIGLATIMKMTELYLLQDTSAGNVQTWEFLKNRMDEAVQLQMALSQTEGMTHTFQRSFNSAFITARNILGLGFKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTW0</accession>
    <name>A0A6I8UTW0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360807.2">
        <property type="nucleotide sequence ID" value="XM_001360770.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTW0"/>
    <dbReference type="KEGG" id="dpo:4804212"/>
    <dbReference type="InParanoid" id="A0A6I8UTW0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39945">
        <property type="entry name" value="FI14129P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39945:SF1">
        <property type="entry name" value="FI14129P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026106011" evidence="1">
        <location>
            <begin position="28"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360807.2"/>
        </source>
    </evidence>
    <sequence length="109" mass="12384" checksum="F22861309D5EEC14" modified="2020-08-12" version="1">MAQKMFHNILLLIATLLLITLNDGVEARPSPEESKTKMLVDPSEYNGDLSVETIMKVQQCEMDTATMELCMRCAKVTKSEIIYPMCCSNDDGIKNWCHSYVYYGNDEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTZ3</accession>
    <name>A0A6I8UTZ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RpL12</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360891.1">
        <property type="nucleotide sequence ID" value="XM_001360854.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTZ3"/>
    <dbReference type="FunCoup" id="A0A6I8UTZ3">
        <property type="interactions" value="1213"/>
    </dbReference>
    <dbReference type="GeneID" id="4804311"/>
    <dbReference type="KEGG" id="dpo:4804311"/>
    <dbReference type="InParanoid" id="A0A6I8UTZ3"/>
    <dbReference type="OMA" id="QPPHDVI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076597">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="8">
        <location>
            <begin position="13"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="7">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360891.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="17710" checksum="34408CF3EA7E2799" modified="2020-08-12" version="1">MPPKFDPTEVKLVYMRCVGGEVGATSSLAPKIGPLGLSPKKVGDDIAKATSDWKGLKITVCLTIQNRQATISVVPSAASLIIKALKEPPRDRKKQKNIKHSGNIAFEDILAIARTMRPRSMARELKGTCKEVLGTAQSVGCTVDGKHPHDVIDELNDGSLEVPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU24</accession>
    <name>A0A6I8UU24_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Not11</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360886.2">
        <property type="nucleotide sequence ID" value="XM_001360849.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU24"/>
    <dbReference type="FunCoup" id="A0A6I8UU24">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="GeneID" id="4804306"/>
    <dbReference type="KEGG" id="dpo:4804306"/>
    <dbReference type="InParanoid" id="A0A6I8UU24"/>
    <dbReference type="OMA" id="NTDIAHQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072413">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019312">
        <property type="entry name" value="CNOT11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10155">
        <property type="entry name" value="CNOT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001360886.2"/>
        </source>
    </evidence>
    <sequence length="228" mass="26060" checksum="210C7D59A31802E3" modified="2020-08-12" version="1">MFLSIAPPLMDFEDELLWINQSNSGKLKILYDKSNYVTPNTKLLIEQAFIQPLSLQDQQILFDDLLKQNSDITHQYGLTPAKLPLLVENNPLISIEILLRLMMTSDITEYFNILVNMDITLHSMEVVNRLTTSGPLPTEFIHLYISNCISTCETVKDKYMQSRLVRLVCVFLQSLIRNKIVNVKELFIEIEAFCVGFSKIKEAAALYRLIKHLETGESALSSNSLSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUU4</accession>
    <name>A0A6I8UUU4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4804719</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361237.5">
        <property type="nucleotide sequence ID" value="XM_001361200.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUU4"/>
    <dbReference type="FunCoup" id="A0A6I8UUU4">
        <property type="interactions" value="2278"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804719"/>
    <dbReference type="InParanoid" id="A0A6I8UUU4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13290">
        <property type="entry name" value="PH_ORP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000001">
        <property type="entry name" value="Oxysterol-binding OBPalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000450">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000014">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF200">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="25"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="323"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="367"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361237.5"/>
        </source>
    </evidence>
    <sequence length="774" mass="86521" checksum="FA110D8286E5FDF2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUZ0</accession>
    <name>A0A6I8UUZ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4804460</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001361019.3">
        <property type="nucleotide sequence ID" value="XM_001360982.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUZ0"/>
    <dbReference type="FunCoup" id="A0A6I8UUZ0">
        <property type="interactions" value="710"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804460"/>
    <dbReference type="InParanoid" id="A0A6I8UUZ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="94"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361019.3"/>
        </source>
    </evidence>
    <sequence length="285" mass="32985" checksum="37166931F8CE3818" modified="2020-08-12" version="1">MKIRSNPLPRRLADVLCFLLIGVFLPIVFMFEIIVVLPAFHEPGGFFHTFTFLMAMFLVFNIKGNMIACMMIDTSVNVKQVEPPPEQSVERLEWRECTECNRLAPPRSWHCKICGACILKRDHHCLYTGCCIGHRNHRFFMGFIFYLFVGSVYALVYNSIYMWIIHSSIYCNWLTVLKLTCPMLLLVTGGFWTNIYLLFYSLNVLALAYGILLLGYHVPIVLRGGVSADRTKQRKLKYKRGVRQNLRSVFGVRMHIAWLSPLIRSDLPDDGYHWRASTSTKKSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV35</accession>
    <name>A0A6I8UV35_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="3">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RnrS</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001361381.2">
        <property type="nucleotide sequence ID" value="XM_001361344.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV35"/>
    <dbReference type="SMR" id="A0A6I8UV35"/>
    <dbReference type="FunCoup" id="A0A6I8UV35">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="GeneID" id="4804895"/>
    <dbReference type="KEGG" id="dpo:4804895"/>
    <dbReference type="InParanoid" id="A0A6I8UV35"/>
    <dbReference type="OMA" id="LEPMFLG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081434">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.620.20:FF:000004">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase subunit M2 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030475">
        <property type="entry name" value="RNR_small_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF18">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00368">
        <property type="entry name" value="RIBORED_SMALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001361381.2"/>
        </source>
    </evidence>
    <sequence length="391" mass="45017" checksum="CCAA0EB31B7F7C37" modified="2020-08-12" version="1">MSQVASKENIADNMEKFSLKSPSKKVLTENASNVRKMSFGNEENGAVKEADPEKPAQSIMEKTGSPFDPSIEPLLRENPRRFVIFPIQYHDIWQMYKKAEASFWTVEEVDLSKDATDWARLTDNERYFISHVLAFFAASDGIVNENLVERFSQEVQITEARCFYGFQIAMENVHSEMYSVLIDTYIREPQERDYLFNAIETMPAVKRKADWAMSWISSKSANFAERIIAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELISRDEGLHCDFAVLMYHHLVQRPKRERIIEIIRDAVEIEQEFLTEALPVNLIGMNCVLMSQYIEFVADRLLGELGVGKIYNTKNPFNFMEMISLDGKTNFFEKKVAEYQRMGVVTSRLDNVFTLDADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW11</accession>
    <name>A0A6I8UW11_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD repeat-containing protein 43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4805044</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361509.3">
        <property type="nucleotide sequence ID" value="XM_001361472.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW11"/>
    <dbReference type="FunCoup" id="A0A6I8UW11">
        <property type="interactions" value="2162"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805044"/>
    <dbReference type="InParanoid" id="A0A6I8UW11"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052414">
        <property type="entry name" value="U3_snoRNA-assoc_WDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="5">
        <location>
            <begin position="438"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="563"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="571"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361509.3"/>
        </source>
    </evidence>
    <sequence length="629" mass="69547" checksum="3E9AF226419DCEC7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW75</accession>
    <name>A0A6I8UW75_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polynucleotide 5'-hydroxyl-kinase NOL9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4805357</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Clp1 family. NOL9/GRC3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361771.5">
        <property type="nucleotide sequence ID" value="XM_001361734.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW75"/>
    <dbReference type="FunCoup" id="A0A6I8UW75">
        <property type="interactions" value="940"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805357"/>
    <dbReference type="InParanoid" id="A0A6I8UW75"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000448">
        <property type="term" value="P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01983">
        <property type="entry name" value="SIMIBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057570">
        <property type="entry name" value="NOL9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057573">
        <property type="entry name" value="NOL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24419">
        <property type="entry name" value="Cupin_NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25467">
        <property type="entry name" value="NOL9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NOL9 N-terminal" evidence="12">
        <location>
            <begin position="365"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="11">
        <location>
            <begin position="542"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="NOL9 C-terminal" evidence="13">
        <location>
            <begin position="761"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="312"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001361771.5"/>
        </source>
    </evidence>
    <sequence length="896" mass="100851" checksum="C17E29A413F41263" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWE4</accession>
    <name>A0A6I8UWE4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bcl-2-related ovarian killer protein homolog B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Debcl</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361966.2">
        <property type="nucleotide sequence ID" value="XM_001361929.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWE4"/>
    <dbReference type="FunCoup" id="A0A6I8UWE4">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="GeneID" id="4805585"/>
    <dbReference type="KEGG" id="dpo:4805585"/>
    <dbReference type="InParanoid" id="A0A6I8UWE4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077322">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.437.10:FF:000009">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF63">
        <property type="entry name" value="BG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="5">
        <location>
            <begin position="148"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361966.2"/>
        </source>
    </evidence>
    <sequence length="302" mass="33356" checksum="C4E31840826E7545" modified="2020-08-12" version="1">MPSTMAPTTSPTAPKLAKFKSSSLDLEIYSANRRGTIATAASDWKALRSSPSNGRSLHAGGGHGLGLGPGLSSMTRAASTSSLASSTRTMTNYQEYKMEIINQGKCLCGQYIRARLRRAGVLNRKVTQRLRNILEPSSHVVYEVFPALNSMGEELERMHPRVYTNISRQLSRAPFGELEDGDMAPMLLNLVAKDLFRSSITWGKIISIFSVCGGFAIDCVRQGHYDYLQCLVDGLAEIIEDDLVYWLIDNGGWLGLQQHIRPRVGEFTFLGWLTLFVTISAGAYMVSNVCKRIGCQLYSLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXC4</accession>
    <name>A0A6I8UXC4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein arginine N-methyltransferase 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Histone-arginine N-methyltransferase PRMT6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6902828</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132564.2">
        <property type="nucleotide sequence ID" value="XM_002132528.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXC4"/>
    <dbReference type="FunCoup" id="A0A6I8UXC4">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902828"/>
    <dbReference type="InParanoid" id="A0A6I8UXC4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXC4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF73">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="8">
        <location>
            <begin position="379"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="9">
        <location>
            <begin position="484"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="61"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002132564.2"/>
        </source>
    </evidence>
    <sequence length="686" mass="78175" checksum="00325534D46220E5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXK8</accession>
    <name>A0A6I8UXK8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6903009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132731.2">
        <property type="nucleotide sequence ID" value="XM_002132695.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXK8"/>
    <dbReference type="KEGG" id="dpo:6903009"/>
    <dbReference type="InParanoid" id="A0A6I8UXK8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002132731.2"/>
        </source>
    </evidence>
    <sequence length="92" mass="9837" checksum="F8C9F44E003C4D33" modified="2020-08-12" version="1">MVCRFNPFYVGPTPPTCCTSDTIKTCDCPPCSPETGYQEPRCQCDLCCSGKNECAPKPEAKAQPATAKGFSKCTATTKTMMCSPACLEPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY74</accession>
    <name>A0A6I8UY74_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Accessory gland protein Acp29AB-like isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6902626</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133152.3">
        <property type="nucleotide sequence ID" value="XM_002133116.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY74"/>
    <dbReference type="FunCoup" id="A0A6I8UY74">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902626"/>
    <dbReference type="InParanoid" id="A0A6I8UY74"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026017101" evidence="2">
        <location>
            <begin position="20"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="130"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133152.3"/>
        </source>
    </evidence>
    <sequence length="259" mass="30237" checksum="6E51E4FD62479838" modified="2020-08-12" version="1">MLCSIVFILFAITFGRSLSQHCEVDETQHECGGYCYGAVKPVLEYLAILQKRVENCEAKQPADTLSKIAKMDEKLKTLGRVLAIQPKSERSSQERKLAQIDEKLAALGRQMELEKKTLMRLQRLVLFEKIGSKYYYIENEITTNWRDALHKCGEIEAHLVSLQSEDEMKVLRRKLDKNKRYWTDLNDLKTEGEFISATTGLKGTFFHWQEPEPNDMENSEDCVEIRTFSDKDWMNDQSCFERIYFICEASWDDPFDMTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYI6</accession>
    <name>A0A6I8UYI6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6902394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133329.3">
        <property type="nucleotide sequence ID" value="XM_002133293.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYI6"/>
    <dbReference type="KEGG" id="dpo:6902394"/>
    <dbReference type="InParanoid" id="A0A6I8UYI6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UYI6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16021">
        <property type="entry name" value="ALP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000017">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF9">
        <property type="entry name" value="DUF229 DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002133329.3"/>
        </source>
    </evidence>
    <sequence length="726" mass="84907" checksum="10CF5682256AF5A4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ08</accession>
    <name>A0A6I8UZ08_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nuclear envelope integral membrane protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6902228</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133756.3">
        <property type="nucleotide sequence ID" value="XM_002133720.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ08"/>
    <dbReference type="FunCoup" id="A0A6I8UZ08">
        <property type="interactions" value="1294"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902228"/>
    <dbReference type="InParanoid" id="A0A6I8UZ08"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ08">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019358">
        <property type="entry name" value="NEMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598:SF1">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10225">
        <property type="entry name" value="NEMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026114165" evidence="10">
        <location>
            <begin position="23"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="418"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002133756.3"/>
        </source>
    </evidence>
    <sequence length="450" mass="51718" checksum="249D85A5E6495673" modified="2020-08-12" version="1">MFQPRLRCGFISLFTLMAICTAATPHPMTDVVFLAPRTSIDITANWNGPNTLRTYCYPGKPPSLLSLFETVEFLLDIGNDDYTEYRGRTPKEVLDHYNEQRSLFSFTLFSQKRQRIHLSPFKPECIGVSSRQPYNVRLRHAQFDVLRFLQFGLGVLVFCSSRTLAKNSIFYYLAGIVLGICGSLLLIISLTSKLFPRRPMMYGVLIGGWTIGVYILKQLADNMCLILLTYRDYVVWYLIITSLISFLVCYRIGPPKNSRSQNIIMWLLQAVGGGLAYFSSWNSSGVIFLIVSAFAAYYFPQSLVVYGKSLYRRRFPPKRRLLTQEEYYQQTVNETQKSLAELREFVNSPNCNQWNIMSNLRNPIRFASFANGEPHLFDEEIEDYSRTIEESMEAADQNEAEDYLQCSMHYRPLSNNGFNVSQDHRRRPPMPPPFTGLPTRHATCEHSAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ36</accession>
    <name>A0A6I8UZ36_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RPB5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6902289</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively. In RNA Pol II, this subunit is present in 2-fold molar excess over the other subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133816.3">
        <property type="nucleotide sequence ID" value="XM_002133780.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ36"/>
    <dbReference type="KEGG" id="dpo:6902289"/>
    <dbReference type="InParanoid" id="A0A6I8UZ36"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ36">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1340.10:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="11">
        <location>
            <begin position="8"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="10">
        <location>
            <begin position="141"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002133816.3"/>
        </source>
    </evidence>
    <sequence length="214" mass="24761" checksum="18F53E2DA3D203C3" modified="2020-08-12" version="1">MSSEMNDEAEVYKLWHIRKTLFEMSHDRGYLVTSAELDQTVEQFTELFGDKPSEQRPMRSDLTMLLSHADDPKNRMLVFFPEEPKVAVKTIKAYAHRMEGEGIHRAILVVRDCLTPPAKQSIMAMAPLYIMEQFLESELTINVTKHELVPQHVVLSDVEKKQLFRCYRLKESQMMRMLSSDPVARYYGLKPGQVVKIVRASESAGKYISYRIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V027</accession>
    <name>A0A6I8V027_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901248</name>
    </gene>
    <organism evidence="3 4">
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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            <begin position="1"/>
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    <sequence length="572" mass="65523" checksum="2BAB6B074E4FDBD9" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <sequence length="426" mass="50142" checksum="23E7CD04D32662AD" modified="2020-08-12" version="1">MESHASAEGFCLCHLMSVWHTIGRIGLYLGYYNVGYAEQRRRFVGDEQLYVHLMALVNLLASLAYLSLSQLWTYEPFVFLLYVPLYVYFLLLRRHLTELLNECATTHRRIQRVLGALFCVRIRRECSYTLASIVMLVSLLSWQLRMYSFYQSCFIAGVAFIYHLQLLFFGSYLLWLSCIYRSLNAFLWRHMRSDRLGLLRALLREQSAIWRLHRRVTRYFGLHFVSFMALPGIRLLLMLQHGTGRQLSLQLVYVVLLLLLQALLLLIGLNLQRQRRRFQRNFLRLRDDPNLFVLRSWRLLQHRTLPQAFGVTLMRRRQHLRRNQDIVTTMFSTNFAATQLVPHPLLLSSVGCKAFSLALTLATFRALLVSHLRELLLLLVLRLLLHKHFNSDESGEGEGPFDDDSNGNVTQTISQVYRFYFYDEFE</sequence>
</entry>
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    <accession>A0A6I8V1X8</accession>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="580"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="654"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="684"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="712"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="736"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="749"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="770"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="790"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="820"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1016"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1031"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1041"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1071"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1087"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002135203.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015043409.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033238539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033238540.1"/>
        </source>
    </evidence>
    <sequence length="1124" mass="120960" checksum="5412E826145AD538" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V264</accession>
    <name>A0A6I8V264_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein FMC1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6896827</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137030.3">
        <property type="nucleotide sequence ID" value="XM_002136994.3"/>
    </dbReference>
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        <property type="interactions" value="629"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A6I8V264"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20271">
        <property type="entry name" value="Complex1_LYR_FMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037667">
        <property type="entry name" value="FMC1_homologue"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31716">
        <property type="entry name" value="PROTEIN FMC1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31716:SF1">
        <property type="entry name" value="PROTEIN FMC1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137030.3"/>
        </source>
    </evidence>
    <sequence length="101" mass="11593" checksum="D4DCC324BC1758D9" modified="2020-08-12" version="1">MSATKVLRSLLHELRQASPNGCIKDSLAARYILAQYKKYETTDQQLCKARNEAVFLGQTYLTYLSSLRNYNELYKEYHGRGERSVKETADVVGFKLPTDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2K8</accession>
    <name>A0A6I8V2K8_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Protein suppressor of variegation 3-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6897307</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137462.1">
        <property type="nucleotide sequence ID" value="XM_002137426.3"/>
    </dbReference>
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    <dbReference type="GeneID" id="6897307"/>
    <dbReference type="KEGG" id="dpo:6897307"/>
    <dbReference type="InParanoid" id="A0A6I8V2K8"/>
    <dbReference type="OMA" id="FCRACHC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247909">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="BESS_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02944">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51031">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BESS" evidence="3">
        <location>
            <begin position="273"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002137462.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="39870" checksum="60AC6811954E785B" modified="2020-08-12" version="1">MSFSENTSVLEWKEGRVKLVRKIKQSVKQENDKKVGGAGAGKPKYNQRFCEKWLNLFEPWLKRSDDDPNKPFCRACHCRLDCNRCHLVRHERTTKHKRNLEALITKGEGAIRNELTVRQERSKYFQKRPMQVLKQASPAKSDVNEPDPLEAVSPEPIATTVHVITTEIDALPSGIDHASHSITCPVPKQEILSESEANNKPNVESHSGILQEANALGNDSAMTTKRTTGSSASNQEGLKLLMQIHQDKNELMESFRELMGSSQSTHPPPPKEKNHVDLFFESVSSSVKALSPKLMAEAKMRVSQLICELELRGLNDNSTNGDVIEMPLETPPASLYLLRQSSSGCALVQQPHFDGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V317</accession>
    <name>A0A6I8V317_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein glob2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">glob2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137543.2">
        <property type="nucleotide sequence ID" value="XM_002137507.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V317"/>
    <dbReference type="FunCoup" id="A0A6I8V317">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="GeneID" id="6897391"/>
    <dbReference type="KEGG" id="dpo:6897391"/>
    <dbReference type="InParanoid" id="A0A6I8V317"/>
    <dbReference type="OMA" id="HGHATAM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247856">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137543.2"/>
        </source>
    </evidence>
    <sequence length="218" mass="24849" checksum="17C4316478374412" modified="2020-08-12" version="1">MGSQINVELSEEQRPTIVLPVYPKPLPERDVSHKLDDNGFSILEKSALRNAWRFIEPFQRRYGQNTFYDFLTEHELLINTFRQQGKINLTKLHGHATAMMRLLSKLVQTLDMNLQFRSSLDENLPFHLKTGIDLDDMKMLANALKDYLLSSPVIEKHNSCTLTTALEKLVTIVGGYAEAEEARKKAMSGFYRNTNTSVEPKSERASSIKSAKKSVIMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3D4</accession>
    <name>A0A6I8V3D4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein btsz isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">btsz</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137209.4">
        <property type="nucleotide sequence ID" value="XM_002137173.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V3D4">
        <property type="interactions" value="32"/>
    </dbReference>
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    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 7B2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137756.2">
        <property type="nucleotide sequence ID" value="XM_002137720.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3G2"/>
    <dbReference type="FunCoup" id="A0A6I8V3G2">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="GeneID" id="6897612"/>
    <dbReference type="KEGG" id="dpo:6897612"/>
    <dbReference type="InParanoid" id="A0A6I8V3G2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247732">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046883">
        <property type="term" value="P:regulation of hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007945">
        <property type="entry name" value="Secretogranin_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12738">
        <property type="entry name" value="NEUROENDOCRINE PROTEIN 7B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12738:SF0">
        <property type="entry name" value="NEUROENDOCRINE PROTEIN 7B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05281">
        <property type="entry name" value="Secretogranin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026025582" description="Neuroendocrine protein 7B2" evidence="9">
        <location>
            <begin position="30"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002137756.2"/>
        </source>
    </evidence>
    <sequence length="273" mass="30018" checksum="3D7B8DD648A2CB62" modified="2020-08-12" version="1">MRNMLSRSQSLKMLIYMLLLVSGGSQVQSYGSKDNIFADVLLTDLLNRMDNDMQVGYYDVDNDGDMAAVAPKDNVDLVSRSEYSRLCDGGSDCVLQSAVNSNPSLRDHEFLQHSSLWGHQFISGGMGEGPNRFNNIVKNDAGLPAYCNPPNPCPEGYDMDSQGGSCLMDFENTAIFSREFQAAQDCTCDNEHMFDCSEQDSADSGSDKGDLNSAVEQYILQMGQENSLNNVNSLVKKAGYPAMPDPRMDAVMNPFLQGDRLPIAAKKGNMLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3Z8</accession>
    <name>A0A6I8V3Z8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein mono-ADP-ribosyltransferase Parp16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Parp16</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138310.2">
        <property type="nucleotide sequence ID" value="XM_002138274.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3Z8"/>
    <dbReference type="FunCoup" id="A0A6I8V3Z8">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898244"/>
    <dbReference type="InParanoid" id="A0A6I8V3Z8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041400">
        <property type="entry name" value="PARP16_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18084">
        <property type="entry name" value="ARTD15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARP16 N-terminal" evidence="1">
        <location>
            <begin position="56"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138310.2"/>
        </source>
    </evidence>
    <sequence length="337" mass="38343" checksum="6B3AD975EB3DA642" modified="2020-08-12" version="1">MSHKIFELGWKSRGHYQGGSGSLALLFEPNPRPRPTSCQRMEWEYNICTWAMLQLRLKADLQGCAALWDIFAAAALNYRRDSRLLPSFPKRFLEPNVDLLCHTVADVPPLEGLLQEVNESGCYQVEYHVVQLLYTVLVEQGDIATLRTIDKEKIELLYDHLAVPTPKTSPTHVFEVARGRKMSRRRQVYEALKANHRGAVCCGFLACKPEMVYALIHREVLPSPMVLAIDADEALALSEYCSGGSPFRYVAIVEFLLLDNEMTHDMRYVIISNPKTMEIGYLVFYDRSCDGKLQEEATEISRFQSFGLEAASSTIYYGRKMYTFLRAGLEAMGLRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4A9</accession>
    <name>A0A6I8V4A9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein F12A10.7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137804.2">
        <property type="nucleotide sequence ID" value="XM_002137768.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4A9"/>
    <dbReference type="KEGG" id="dpo:6897666"/>
    <dbReference type="InParanoid" id="A0A6I8V4A9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026140615" evidence="1">
        <location>
            <begin position="22"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137804.2"/>
        </source>
    </evidence>
    <sequence length="108" mass="11356" checksum="B5F8D1FA35FFC822" modified="2020-08-12" version="1">MRCVILLGLIACLACLSMAWGLEESSNDQDLSLSPVEQGIEQAAEGQVRAKRQFYGGRPYYGGGFGGFGRPYGGGFGGGYYGRPYYGGYGRPYGGGFGGPFYGGGFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4B8</accession>
    <name>A0A6I8V4B8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4780 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137831.3">
        <property type="nucleotide sequence ID" value="XM_002137795.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4B8"/>
    <dbReference type="KEGG" id="dpo:6897705"/>
    <dbReference type="InParanoid" id="A0A6I8V4B8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031961">
        <property type="entry name" value="DUF4780"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16012">
        <property type="entry name" value="DUF4780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4780" evidence="1">
        <location>
            <begin position="175"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137831.3"/>
        </source>
    </evidence>
    <sequence length="371" mass="40823" checksum="248ACAF220DDE718" modified="2020-08-12" version="1">MRPVPIGLVCSQGEIAGLYPRGASAIPVRLVEYFRTCRVSGGYDTVDLFTPTLVGISMSNQNIKTNIIIKQEVEDKEEAAQKINRAAQPGGVGDPLTKGLKKKALKRYNRYLREGMLPKEARMRAINPNGYQKAASKKKTTVAPPAVKGGLKRKIPSQATTVAPKRAKKAREGRGPTDMAILATRYPLRLLTKNEAAIVEKELVDEMLKGSSHSINFDGIHFKTGLIVVKCRDDRSKQWLELTVPKVCGIGKVQLRTCPMDDLPSMKIVTIEIPDAAEQKVQAAVRLLRKQNSDLDSDAWILLKCSNTKGGGRSITLGLQQQALELLQKNGMKMSYRFGQLPCRLEADHSGDGDGGENAMWYVDIPKISDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5D7</accession>
    <name>A0A6I8V5D7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138387.1">
        <property type="nucleotide sequence ID" value="XM_002138351.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5D7"/>
    <dbReference type="GeneID" id="6898332"/>
    <dbReference type="KEGG" id="dpo:6898332"/>
    <dbReference type="InParanoid" id="A0A6I8V5D7"/>
    <dbReference type="OMA" id="NCYQNYG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246126">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138387.1"/>
        </source>
    </evidence>
    <sequence length="69" mass="7814" checksum="E56323069338377F" modified="2020-08-12" version="1">MKVSIFGELNTFELLVLSSLLPGVFVFLWTLITGDIKNFKGSKLAYSEFTAQDPINCYQNYGEKKDKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V617</accession>
    <name>A0A6I8V617_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein FAM76B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM76 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138817.1">
        <property type="nucleotide sequence ID" value="XM_002138781.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V617"/>
    <dbReference type="GeneID" id="6898846"/>
    <dbReference type="KEGG" id="dpo:6898846"/>
    <dbReference type="InParanoid" id="A0A6I8V617"/>
    <dbReference type="OMA" id="DYRLYLC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245580">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032017">
        <property type="entry name" value="FAM76"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16046">
        <property type="entry name" value="FAM76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15288" checksum="D1DB28DEB1E6534E" modified="2020-08-12" version="1">MDPVVVEIVDAPDDRWYLCTKCFQRFLWVDLSSRQLRCRRCRLADKQCAICDQLFEPTNGQQQYCKRCDYFLMQHAFGQKPPPTREKAELLLQLQLQLQLKTPAGHNPSVTERWREIKCAAGLAEDVNLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6V5</accession>
    <name>A0A6I8V6V5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801409</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736503.3">
        <property type="nucleotide sequence ID" value="XM_003736455.3"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V6V5">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V6V5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029717">
        <property type="entry name" value="FAM193"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15109">
        <property type="entry name" value="AGAP004327-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15109:SF4">
        <property type="entry name" value="FAM193 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="695"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="928"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1016"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1168"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1227"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1381"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1478"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1555"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1983"/>
            <end position="2005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2055"/>
            <end position="2106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2125"/>
            <end position="2154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2216"/>
            <end position="2243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2326"/>
            <end position="2371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2535"/>
            <end position="2568"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1290"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1025"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1054"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1168"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="1227"/>
            <end position="1237"/>
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        <location>
            <begin position="1240"/>
            <end position="1249"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1381"/>
            <end position="1391"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1400"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1560"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1570"/>
            <end position="1587"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1588"/>
            <end position="1607"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1608"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1654"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1669"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1685"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1712"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1738"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1764"/>
            <end position="1785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1803"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1830"/>
            <end position="1841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1858"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2055"/>
            <end position="2064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2065"/>
            <end position="2074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="2075"/>
            <end position="2087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="2336"/>
            <end position="2357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_003736503.3"/>
        </source>
    </evidence>
    <sequence length="2651" mass="286452" checksum="985E9F4FDC72348C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VA89</accession>
    <name>A0A6I8VA89_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Gastrula zinc finger protein XlCGF7.1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6902419</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037014.2">
        <property type="nucleotide sequence ID" value="XM_015181528.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VA89"/>
    <dbReference type="KEGG" id="dpo:6902419"/>
    <dbReference type="InParanoid" id="A0A6I8VA89"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001119">
        <property type="entry name" value="zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000188">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="15">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015037014.2"/>
        </source>
    </evidence>
    <sequence length="324" mass="37720" checksum="989A23EEB506696B" modified="2020-08-12" version="1">MQRPYMRMEEICRVCMGTSGEFKNIFDERPTMDTCIGDMISQCTGYVVKRGDSLPENICPPCLEDAMSAFNLKSTYEQSHKLFFSLIEGDKEQDEDSLQSADRTEQSKHQDDSNNSDNCKRVFKCSVCPKSFQRKYHLSEHSHSHTGERPYTCPHCAKSFSQSRILRNHIRTHTDERSFYCAHCSKSFKLKGQLIVHSRTHTGERPYKCSKCAVSFKQLAHLTSHVFSHTGERPHKCSHCPKTFTQVRSLNMHIRTHTGERPYKCTECTRSFIQKYHLKEHIRKHTGERPYQCYHCSKTFTQESTFKKHIRTQHGTIPPLLNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAC4</accession>
    <name>A0A6I8VAC4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC6897093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037072.2">
        <property type="nucleotide sequence ID" value="XM_015181586.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233067.1">
        <property type="nucleotide sequence ID" value="XM_033377176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAC4"/>
    <dbReference type="KEGG" id="dpo:6897093"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015037072.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033233067.1"/>
        </source>
    </evidence>
    <sequence length="186" mass="21120" checksum="EE5391C4E4D55AE2" modified="2020-08-12" version="1">MNDSDSDDTSFHSFDGSELSDFFNVSSCSSDEDEVPLYSKNKSLRKLPPVPYELPSVEVFSLNRASSMLKESIESNTNRPDEAKEKNLPTDNSQNTQPECFWSPRKQFDLVLKSYFRHTKPVEEEYVSPFKPNMSIQPRPKESPLLKGLKKPIVLPAGYDLSALQVIKDPNHWAIRPGVRTGDIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBH0</accession>
    <name>A0A6I8VBH0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GILT-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC26534041</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037813.2">
        <property type="nucleotide sequence ID" value="XM_015182327.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBH0"/>
    <dbReference type="KEGG" id="dpo:26534041"/>
    <dbReference type="InParanoid" id="A0A6I8VBH0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF68">
        <property type="entry name" value="GH19763P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026155112" evidence="3">
        <location>
            <begin position="21"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015037813.2"/>
        </source>
    </evidence>
    <sequence length="204" mass="23541" checksum="D85AD95C8EE5B714" modified="2020-08-12" version="1">MSQLIFLLLVCHVLPHGCEQDQKRLKMTVLYEVLCVDSVDYLTSLLRVFKKLNGYIDLQLVPYGKASHGGTVCQHGPKECKGNRIQDCALHTDMTQLEKLSYVTCQITNRKLVTKIDTKCIKSSAIKRYITECMGRNGRSRTYQKQSERITNMYEFDQIPAIIYNDEFDESLQDEATDKLKKSICRAIVETGIEEPTYCFKRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBI2</accession>
    <name>A0A6I8VBI2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Salivary secreted peptide</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26531942</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037786.2">
        <property type="nucleotide sequence ID" value="XM_015182300.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBI2"/>
    <dbReference type="KEGG" id="dpo:26531942"/>
    <dbReference type="InParanoid" id="A0A6I8VBI2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031734">
        <property type="entry name" value="MBF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37685">
        <property type="entry name" value="GEO11136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37685:SF1">
        <property type="entry name" value="GEO11136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15868">
        <property type="entry name" value="MBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015037786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13663" checksum="8AB8043D9809BB54" modified="2020-08-12" version="1">MIDLQTIIRLSLVVVALVVVVSPLLVHSKSVVFGQQSPNARLLYATHVAEPSKFFRITERRVTFKQDKNIKIIGAIVIENNNSTIGGEASLIEGGPTYTFATIKFKSKRNHGLNFNLYIYDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGU2</accession>
    <name>A0A6I8VGU2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transporter svop-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4815981</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015041020.2">
        <property type="nucleotide sequence ID" value="XM_015185534.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGU2"/>
    <dbReference type="KEGG" id="dpo:4815981"/>
    <dbReference type="InParanoid" id="A0A6I8VGU2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VGU2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF35">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="53"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015041020.2"/>
        </source>
    </evidence>
    <sequence length="555" mass="60330" checksum="1B4C46A0FD8239E1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VH38</accession>
    <name>A0A6I8VH38_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alanine transaminase</fullName>
            <ecNumber evidence="8">2.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">LOC6902153</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-alanine + 2-oxoglutarate = pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:19453"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.2" evidence="8"/>
    <dbReference type="RefSeq" id="XP_002133684.3">
        <property type="nucleotide sequence ID" value="XM_002133648.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041209.2">
        <property type="nucleotide sequence ID" value="XM_015185723.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VH38"/>
    <dbReference type="KEGG" id="dpo:6902153"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000012">
        <property type="entry name" value="alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000010">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF29">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="199"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002133684.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015041209.2"/>
        </source>
    </evidence>
    <sequence length="593" mass="65266" checksum="306783546D206959" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJ13</accession>
    <name>A0A6I8VJ13_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17 18">PDF receptor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17 18" type="primary">Pdfr</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17 18">MV-25-SWS-2005</strain>
            <tissue evidence="16 17 18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015042348.2">
        <property type="nucleotide sequence ID" value="XM_015186862.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042350.2">
        <property type="nucleotide sequence ID" value="XM_015186864.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238275.1">
        <property type="nucleotide sequence ID" value="XM_033382384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VJ13"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098771">
        <property type="term" value="P:inorganic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040011">
        <property type="term" value="P:locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055080">
        <property type="term" value="P:monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15261">
        <property type="entry name" value="7tmB1_PDFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000029">
        <property type="entry name" value="PDF receptor isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF17">
        <property type="entry name" value="PDF RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="13">
        <location>
            <begin position="122"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="14">
        <location>
            <begin position="236"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="580"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="600"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015042348.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015042350.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033238275.1"/>
        </source>
    </evidence>
    <sequence length="764" mass="83933" checksum="6D3F6DBBA8FD7ECA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLS1</accession>
    <name>A0A6I8VLS1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium uniporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MCU</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The selectivity filter, in which calcium ions are arranged in single file, is composed of two acidic rings separated by one helical turn along the central axis of the channel pore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the MCU (TC 1.A.77) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043914.2">
        <property type="nucleotide sequence ID" value="XM_015188428.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLS1"/>
    <dbReference type="FunCoup" id="A0A6I8VLS1">
        <property type="interactions" value="1107"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900178"/>
    <dbReference type="InParanoid" id="A0A6I8VLS1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015292">
        <property type="term" value="F:uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006769">
        <property type="entry name" value="MCU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039055">
        <property type="entry name" value="MCU_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462:SF10">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04678">
        <property type="entry name" value="MCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 15" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4 15" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium uniporter protein C-terminal" evidence="17">
        <location>
            <begin position="245"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="54"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="475"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015043914.2"/>
        </source>
    </evidence>
    <sequence length="500" mass="57933" checksum="D6797B0470217AEC" modified="2020-08-12" version="1">MSRSGAAVVSAFRLLLRPSGVSRHRGLRLGPVTAFALHLRPCGGHELQQHRTFAATTEDDDKHKTPLETGDTMGHQDGQTPEQEGAQGQESEESEEAKESLEQAQASEEDHPKPKRREIQEYPYPILLDRGPRGSRSKDQQKKDTQETSRNRKKIQRQILDLIERERTKFEPRPMKKNQEYRIKCVGPESGSGKERRTEDDIFVEYVNGMPHMTVRLPSRNELCQFALKPITHNVGDLLSMLRQEDRGIDRAAVINKNGVRIASSCTIDSLLDDTFSIQINNRTMEVNPPKRDKITVESMDKVGDVRKVIAQLYEAFNVGEYQLEKSNQLAKELESIRYELEPLEEKKLELSRKAARRTNFMTWMGLGLMSVQFGILARLTWWEYSWDIMEPVTYFVTYGTTMAMYAYYCVTKREYMMEAVKNREYSLAIYKNAKKIQFDVDHYNQLKRKTAELEYNLRRINDPLNMQLPSHLVRTQENTPPTEEQQQRQPDDPREGKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMJ0</accession>
    <name>A0A6I8VMJ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zwei Ig domain protein zig-8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dpr7</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015044489.2">
        <property type="nucleotide sequence ID" value="XM_015189003.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VMJ0"/>
    <dbReference type="FunCoup" id="A0A6I8VMJ0">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VMJ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244558">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VMJ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000129">
        <property type="entry name" value="CLUMA_CG018772, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001705">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF6">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 7, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="98"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="219"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015044489.2"/>
        </source>
    </evidence>
    <sequence length="359" mass="40202" checksum="F386DA55D6F07B17" modified="2020-08-12" version="1">MLLLSECCQIKKSYVSSTDMNVYTFIYAHMHANYPACNFKTLVLTDMAASFVKSTCLDFSFNKIRPIILLILNFNICVQHISATSFLNINDLASSDRPFFDDISPRNVSAVIDETAVLRCRVKNKGNRTVSWMRKRDLHILTTNIYTYTGDQRFSVIHPPSSEDWDLKIDYAQPRDSGIYECQVNTEPKINLAIILDIAAESEVRDLNSDRRYYDSKAARAKILGSTEIHVKRDSTIALACSVNMHASSVLWYHGSSIVDFDSLRGGISLETEKTDIGTTSRLMLTRASLRDSGNYTCVPNGAIPASVRVHVLTGEQPAAMQTSAAIGMNAHTAMINIISIKFLLNISTLAEYVFRKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPE3</accession>
    <name>A0A6I8VPE3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232906.1">
        <property type="nucleotide sequence ID" value="XM_033377015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPE3"/>
    <dbReference type="KEGG" id="dpo:117183346"/>
    <dbReference type="InParanoid" id="A0A6I8VPE3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232906.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="15924" checksum="4FF43EC63AE8B1C0" modified="2020-08-12" version="1">MHTYAHECVSCADFLACPGAPASLLGLCKIGNFLVHLFLHVSQSEDSGPNRIEDFLLPDAGGWMLILMDIKRQNLNQPTNGGKGMKQKLKLRRRLWTESKEYVMSTFGKTRKGFSPRDSTPPKDKSRMKMGGCGDGCGIFSSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VR58</accession>
    <name>A0A6I8VR58_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6899054</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033233551.1">
        <property type="nucleotide sequence ID" value="XM_033377660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VR58"/>
    <dbReference type="FunCoup" id="A0A6I8VR58">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899054"/>
    <dbReference type="InParanoid" id="A0A6I8VR58"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VR58">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF41">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="549"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="924"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1117"/>
            <end position="1143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033233551.1"/>
        </source>
    </evidence>
    <sequence length="1154" mass="133347" checksum="658106C9F9BF2324" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VS70</accession>
    <name>A0A6I8VS70_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233748.1">
        <property type="nucleotide sequence ID" value="XM_033377857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VS70"/>
    <dbReference type="KEGG" id="dpo:26532956"/>
    <dbReference type="InParanoid" id="A0A6I8VS70"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233748.1"/>
        </source>
    </evidence>
    <sequence length="129" mass="14258" checksum="493279DCB0220A38" modified="2020-08-12" version="1">MSSGINCREIFVFLEILIWNQAKQRNRQNSQHSNNNSGGSKETITSNHKNRCNRRSGNNSNSNTNNNNNNSAVKVIKQQQQQRQPLPLPLTLPLPEAGKNKNSRGSSSNNSSSSTTNSSSNNLHILQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTG0</accession>
    <name>A0A6I8VTG0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Odorant receptor 98a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6898223</name>
        <name evidence="13" type="synonym">LOC117183697</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033234356.1">
        <property type="nucleotide sequence ID" value="XM_033378465.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234424.1">
        <property type="nucleotide sequence ID" value="XM_033378533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTG0"/>
    <dbReference type="KEGG" id="dpo:117183697"/>
    <dbReference type="KEGG" id="dpo:6898223"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033234356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033234424.1"/>
        </source>
    </evidence>
    <sequence length="300" mass="35185" checksum="A26280F90707A9C8" modified="2020-08-12" version="1">MRLNMWRYHKIKELLGRMDKRCINITERLEVHRWVARCNIVYPIYQFMYTAYTLSTFFTAIFSGVLPFHLYNPFIDWRESTMNLWIASVLELIPMNGIVSQTYMMDVFPLLYGLILRCHVKLLRQRIENLCSDPRKSDDENNEDLMFCIKDHKLILEYVSVMRPVIETIIFVQFLLIGLLLGITMLNLFFFADFWAKLAIAAYINGLIIQTFPFCFTCDLLKDDCESLAFAIFHSHWKSSSRRYKSSLIYFLHNAQMPISFTAGSIFPIGLNTNITVAKLAFSVVTFVQQLNIADKLRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VUU4</accession>
    <name>A0A6I8VUU4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-binding protein Rfx5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234852.1">
        <property type="nucleotide sequence ID" value="XM_033378961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VUU4"/>
    <dbReference type="KEGG" id="dpo:4804725"/>
    <dbReference type="InParanoid" id="A0A6I8VUU4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026249549" evidence="1">
        <location>
            <begin position="24"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234852.1"/>
        </source>
    </evidence>
    <sequence length="273" mass="27788" checksum="2FF8078A8558CE8A" modified="2020-08-12" version="1">MTNFIAKKSLLVLSLLLLGTVQAQPLEDHHGREQRQLKGGPGANTDADVKMVANVTPAAATSMQMGMGLSLPMNQMALNAVGTQLVRYPNYPGLIYAPGIAQGSLNGLSGLQTNIANNYPAYPDPNMVGASPGFGAASSFGGAPGFGGTPSFGAAPGFGTGPGFSAAPSFGPAPGFGTAPGFGFGSYPGASSPAALMYGNPMSYPNPQLPNGFVPQPSVDNFSALNNIVNMANVQGLGGSNQGFYQNPQLPGLYAPAGGYMERMNMQGYAPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVQ3</accession>
    <name>A0A6I8VVQ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mucin-5AC isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4801285</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234664.1">
        <property type="nucleotide sequence ID" value="XM_033378773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VVQ3"/>
    <dbReference type="InParanoid" id="A0A6I8VVQ3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052976">
        <property type="entry name" value="Scoloptoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933:SF47">
        <property type="entry name" value="CPAP1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933">
        <property type="entry name" value="FI18007P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026202719" evidence="2">
        <location>
            <begin position="33"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="59"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="639"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="725"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="497"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="639"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="747"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="802"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033234664.1"/>
        </source>
    </evidence>
    <sequence length="835" mass="93268" checksum="1F458E531CA360EF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWW1</accession>
    <name>A0A6I8VWW1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC117183958</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235572.1">
        <property type="nucleotide sequence ID" value="XM_033379681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWW1"/>
    <dbReference type="KEGG" id="dpo:117183958"/>
    <dbReference type="InParanoid" id="A0A6I8VWW1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="551"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033235572.1"/>
        </source>
    </evidence>
    <sequence length="586" mass="66646" checksum="C8867AE37FAAA76A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VX08</accession>
    <name>A0A6I8VX08_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Metalloendopeptidase</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4816346</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="RefSeq" id="XP_033235617.1">
        <property type="nucleotide sequence ID" value="XM_033379726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VX08"/>
    <dbReference type="KEGG" id="dpo:4816346"/>
    <dbReference type="InParanoid" id="A0A6I8VX08"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="8">
        <location>
            <begin position="40"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033235617.1"/>
        </source>
    </evidence>
    <sequence length="252" mass="27890" checksum="41BC2A103A47C797" modified="2020-08-12" version="1">MSSAGLPNLLLAGQPSAKVFDKGLIYMAFMTARPKPFPGRDTTGRVAPSSTASVKACVCIPACSLTAPVGLLAFTASRDTDLIEKAMAAIASQTCVRFRRTRNAQEPQVVIQRKEAGCWSYVGFLGRQQQLLNLGERCMTSGTIQHEILHALGLFHTHCDPRRDQYVKIHPENLKPGYEHNFRLLGIGQDPQMTDFGLGYDYESLMHYGEYAFSRNGLPTIVPLRRGVRIGQAKGLSAKDVRKLNRMYCRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXK8</accession>
    <name>A0A6I8VXK8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Sister chromatid cohesion protein solo-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">LOC4817921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
            <tissue evidence="4 5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235830.1">
        <property type="nucleotide sequence ID" value="XM_033379939.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235831.1">
        <property type="nucleotide sequence ID" value="XM_033379940.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235832.1">
        <property type="nucleotide sequence ID" value="XM_033379941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXK8"/>
    <dbReference type="KEGG" id="dpo:4817921"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077500">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VXK8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="624"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="775"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="332"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235832.1"/>
        </source>
    </evidence>
    <sequence length="945" mass="106305" checksum="82629853E7430176" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXK9</accession>
    <name>A0A6I8VXK9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">UDP-glucuronosyltransferase 2B9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">Ugt37D1</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132612.2">
        <property type="nucleotide sequence ID" value="XM_002132576.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235821.1">
        <property type="nucleotide sequence ID" value="XM_033379930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXK9"/>
    <dbReference type="KEGG" id="dpo:6902878"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VXK9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF159">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634575" evidence="5">
        <location>
            <begin position="33"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002132612.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033235821.1"/>
        </source>
    </evidence>
    <sequence length="543" mass="60949" checksum="BC1516AA0C312C14" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYG5</accession>
    <name>A0A6I8VYG5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4816386</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235569.1">
        <property type="nucleotide sequence ID" value="XM_033379678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYG5"/>
    <dbReference type="FunCoup" id="A0A6I8VYG5">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816386"/>
    <dbReference type="InParanoid" id="A0A6I8VYG5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VYG5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="KEKKON 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026106022" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="28"/>
            <end position="1280"/>
        </location>
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        <location>
            <begin position="647"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1205"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1225"/>
            <end position="1244"/>
        </location>
    </feature>
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        <location>
            <begin position="1259"/>
            <end position="1273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033235569.1"/>
        </source>
    </evidence>
    <sequence length="1280" mass="144913" checksum="B05300EAE59CA9B1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W084</accession>
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        </submittedName>
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        <name evidence="10" type="primary">LOC6897988</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033236760.1">
        <property type="nucleotide sequence ID" value="XM_033380869.1"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="FAM234"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23727">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
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        <property type="entry name" value="Integrin alpha N-terminal domain"/>
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            <begin position="120"/>
            <end position="144"/>
        </location>
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        <location>
            <begin position="211"/>
            <end position="543"/>
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        <location>
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            <begin position="775"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF23727"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033236760.1"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A6I8W0R1</accession>
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        <recommendedName>
            <fullName evidence="4 11">Condensin complex subunit 2</fullName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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        <citation type="submission" date="2025-04" db="UniProtKB">
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                <consortium name="RefSeq"/>
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            <strain evidence="14 15 16">MV-25-SWS-2005</strain>
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        <text evidence="11">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.</text>
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        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CND2 (condensin subunit 2) family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
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        <property type="project" value="InterPro"/>
    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="130"/>
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    </feature>
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        <location>
            <begin position="19"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="143"/>
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    </feature>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR017126"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033236925.1"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033236926.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033236927.1"/>
        </source>
    </evidence>
    <sequence length="706" mass="79109" checksum="BEBF0748491B06C2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W252</accession>
    <name>A0A6I8W252_DROPS</name>
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        <submittedName>
            <fullName evidence="8">Caltractin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC117184243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the centrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236764.1">
        <property type="nucleotide sequence ID" value="XM_033380873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W252"/>
    <dbReference type="SMR" id="A0A6I8W252"/>
    <dbReference type="FunCoup" id="A0A6I8W252">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184243"/>
    <dbReference type="InParanoid" id="A0A6I8W252"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000077">
        <property type="entry name" value="Centrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000070">
        <property type="entry name" value="Centrin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="48"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="157"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="18"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033236764.1"/>
        </source>
    </evidence>
    <sequence length="192" mass="21609" checksum="85AA71640DA48537" modified="2020-08-12" version="1">MESTAAVANIVSPPTAVGTAATTTAAATKRSNQQGRKKSGPKFELSDAQKSDIKEAFDLFDNECTGYIEVKELKVAIRALGFEPKKEEIKRMIAEIDKDGSGRIAFNDFLHLMTMKMAEKDTKEEILKAFRLFDDDETGKISFKNLKRVARELGETLTDEELREMIDEADLDNDGEVNQEEFLRIMKKTSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4I8</accession>
    <name>A0A6I8W4I8_DROPS</name>
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        <recommendedName>
            <fullName evidence="3">Venom dipeptidyl peptidase 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">ome</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10">MV-25-SWS-2005</strain>
            <tissue evidence="9 10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353204.3">
        <property type="nucleotide sequence ID" value="XM_001353168.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238233.1">
        <property type="nucleotide sequence ID" value="XM_033382342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4I8"/>
    <dbReference type="KEGG" id="dpo:4813899"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF192">
        <property type="entry name" value="IP17501P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="7">
        <location>
            <begin position="366"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="6">
        <location>
            <begin position="827"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="32"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353204.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033238233.1"/>
        </source>
    </evidence>
    <sequence length="1039" mass="116422" checksum="D88B5F17AA2FB144" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W523</accession>
    <name>A0A6I8W523_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC6901816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238433.1">
        <property type="nucleotide sequence ID" value="XM_033382542.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238434.1">
        <property type="nucleotide sequence ID" value="XM_033382543.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901816"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W523">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="652"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="700"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="740"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="771"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="782"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="795"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="808"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="819"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="873"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="897"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="950"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="974"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1027"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1051"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1140"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033238434.1"/>
        </source>
    </evidence>
    <sequence length="1341" mass="155196" checksum="936126A302251F5F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7T6</accession>
    <name>A0A6I8W7T6_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Microfibril-associated glycoprotein 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4814291</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239342.1">
        <property type="nucleotide sequence ID" value="XM_033383451.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome X"/>
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        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF327">
        <property type="entry name" value="FI21813P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026322271" evidence="2">
        <location>
            <begin position="23"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="143"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239342.1"/>
        </source>
    </evidence>
    <sequence length="363" mass="39695" checksum="7C5A682BD447D119" modified="2020-08-12" version="1">MSQMLQLDALVMAMACLTTTQTDLGGGLGMVPPILDNSVSHRGFYIDLSLSYLYLQTSCPISALSGLTTRLQALTNDIASVKEQIGGLQEQLVDLRRSGGPAGYPVSLGLPNAIGSRLPISSLSSLDVDVSQLLAAQGGPGAGGDPKTPSNCLKQQGGVVRIQTRANVEPFFVFCENNVSGGGWTMVVNRYDGSEDFNRKWADYKIGFGSITTEFFIGLDKLHQILSSDNYELLVQLQNRKQELRYALYDRFGIGSESEQYHLSVLGKYKGDAADALRQHEGKKFSTQDRDNDESEANCAAAQSAAFWYGSSCNLSNPFGLYQRLLDRDKDEYKGILWRGFLDGPKGSLKIVRMMVRPRNGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7Y6</accession>
    <name>A0A6I8W7Y6_DROPS</name>
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        <recommendedName>
            <fullName evidence="5">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Grip128</name>
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    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239490.1">
        <property type="nucleotide sequence ID" value="XM_033383599.1"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:gamma-tubulin complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GCP5_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR059169">
        <property type="entry name" value="GCP5_N_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="841"/>
            <end position="1080"/>
        </location>
    </feature>
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        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="660"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="708"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="660"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="673"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="711"/>
            <end position="723"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033239490.1"/>
        </source>
    </evidence>
    <sequence length="1088" mass="125142" checksum="945F45673783C8DF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W978</accession>
    <name>A0A6I8W978_DROPS</name>
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        <recommendedName>
            <fullName evidence="3">DUF4729 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6901665</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239827.1">
        <property type="nucleotide sequence ID" value="XM_033383936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W978"/>
    <dbReference type="KEGG" id="dpo:6901665"/>
    <dbReference type="InParanoid" id="A0A6I8W978"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031732">
        <property type="entry name" value="DUF4729"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15866">
        <property type="entry name" value="DUF4729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4729" evidence="3">
        <location>
            <begin position="382"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239827.1"/>
        </source>
    </evidence>
    <sequence length="572" mass="63558" checksum="73042003719CB051" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9A9</accession>
    <name>A0A6I8W9A9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4744 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239857.1">
        <property type="nucleotide sequence ID" value="XM_033383966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9A9"/>
    <dbReference type="KEGG" id="dpo:117184872"/>
    <dbReference type="InParanoid" id="A0A6I8W9A9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031806">
        <property type="entry name" value="DUF4744"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15918">
        <property type="entry name" value="DUF4744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4744" evidence="1">
        <location>
            <begin position="48"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239857.1"/>
        </source>
    </evidence>
    <sequence length="101" mass="12150" checksum="6DC4866F8CFE5A3B" modified="2020-08-12" version="1">MCRPVCWSRDRLVIVWEPDISNPPFLRRRIKRWASASRRRTYVPYDFPQNTKQYDFHPPHGVLFKHFQALARSEANKSSSFFDLAIDNKDFDYGNHIYIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCQ2</accession>
    <name>A0A6I8WCQ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine incorporator 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Serinc</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240339.1">
        <property type="nucleotide sequence ID" value="XM_033384448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCQ2"/>
    <dbReference type="FunCoup" id="A0A6I8WCQ2">
        <property type="interactions" value="1193"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WCQ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026021195" evidence="7">
        <location>
            <begin position="23"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033240339.1"/>
        </source>
    </evidence>
    <sequence length="469" mass="50511" checksum="5E916D341749E6D9" modified="2020-08-12" version="1">MGAVLGLCSAAQCALCCGGTAASMCCSACPSCTNASSSRFMYAFILLVGTVLGAIALSPGLQDTLKKLPFCINSTSTYSSKAIDTFSGGSLQVDCEYALGYMAVYRVCFGMACFFALMALIMLGVKSSRDPRSHIQNNFWPLKFLICFGAAIGAIFIPDGSFGPAMMWVGLIGGLAFILVQLVIIVDFAHSLAESWIEGAENSRGYYYALAGVTLLGYILSLTGITLLYIYFTTSTGCGINKFFISINLIFCLVISVISILPAVQERLPHSGLLQSSLVTLYTVYLTWSAVANNPEKACNPGMFGLMEGFGNGTTTTIAPPTPPSHNSKVTFDTTNIIGLVVWLLCILYNCISSAVEVSKITHDNSEKRVLTEALSDTEAATDADGKPSADNEVEGVTYSWSMFHIVFVCASLYVMMTLTNWYKPNSDIELFNGNEASMWVKIISSWLGVFIYGWSLAAPIILSNRDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8GTF5</accession>
    <name>A0ABD8GTF5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor SYF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4805311</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">May be part of a spliceosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SYF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361731.1">
        <property type="nucleotide sequence ID" value="XM_001361694.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8GTF5"/>
    <dbReference type="SMR" id="A0ABD8GTF5"/>
    <dbReference type="GeneID" id="4805311"/>
    <dbReference type="KEGG" id="dpo:4805311"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264">
        <property type="entry name" value="GCIP-INTERACTING PROTEIN P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08231">
        <property type="entry name" value="SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361731.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="26422" checksum="2F4879708BB87B08" modified="2025-06-18" version="1">MEQSKSASDKLAERKARLLDLHKKRQEARTDNHQEVVAEDARKKLPKNWEARKRQAEWLLADDKAREDAQAAGKDYERLKLLEVSAIDAERIEKKKKRKDNPDLGFSTYEAQTARQYSRLVKGMPARDMEKYEKQKAELGEAFYGGPHTTLHALTKDTPAAINKMVNDLDQQIERRKKYSRRRIYNDDADVDFINERNSKFNKKLDRFYGEHTAEIKQNLERGTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>B5DJ12</accession>
    <accession>A0A6I8UX91</accession>
    <name>B5DJ12_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor MafB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6903280</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MCRIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132510.1">
        <property type="nucleotide sequence ID" value="XM_002132474.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DJ12"/>
    <dbReference type="FunCoup" id="B5DJ12">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="GeneID" id="6903280"/>
    <dbReference type="KEGG" id="dpo:6903280"/>
    <dbReference type="eggNOG" id="ENOG502S8J7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1779434_0_0_1"/>
    <dbReference type="InParanoid" id="B5DJ12"/>
    <dbReference type="OMA" id="LYCNESD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247248">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029428">
        <property type="entry name" value="MCRIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14799">
        <property type="entry name" value="FAM195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002132510.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="16278" checksum="CE91BA9A68152B4F" modified="2008-10-14" version="1">MERNNGRYPYIRRQGSGHNNYHHHHNNNANSTSSTAGSTNATQYSNHSINNSPSVGMSTGAHNSNAIYAAAVPPSQNVPISQHDELIRYIREAWNKVYEQGTPVYRNESDNQLKNFKPFNLEEYWGQRLVQNIHVTTTNASGHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKF1</accession>
    <accession>A0A6I8UYI8</accession>
    <name>B5DKF1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cytochrome P450 4ae1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Cyp4ae1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133372.2">
        <property type="nucleotide sequence ID" value="XM_002133336.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKF1"/>
    <dbReference type="SMR" id="B5DKF1"/>
    <dbReference type="FunCoup" id="B5DKF1">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DKF1"/>
    <dbReference type="GeneID" id="6901834"/>
    <dbReference type="KEGG" id="dpo:6901834"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_1_1"/>
    <dbReference type="InParanoid" id="B5DKF1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244262">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DKF1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20628">
        <property type="entry name" value="CYP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF187">
        <property type="entry name" value="CYTOCHROME P450 4AE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002133372.2"/>
        </source>
    </evidence>
    <sequence length="507" mass="57240" checksum="8B858FA03CB3BE0A" modified="2016-01-20" version="2">MDSENMLMALLLTMALTLIVAWLLQLALREMRHPLRGFVPSVSRVPLVGAAWQMRSFQPDNLHEKFAEYVERFGRSFMASVCGQVIMVTAEPSHVDALLHSQKQLRKSSIYGALRGWLGNGLLLSQGEQWHGMRKIITPTFHFSILEEFVEVFDKQSQLLVERLQPLSNGGMVAINIYPYVGLAALDIITETAMGVCVNAQLDADSAVVQAVKAVTNTLATRFMRPHLLPPLLFRLCWPSGFRKQQEGIKCLHLFTNSIIEQRRQLLRRENAPGEPVARDRRNALLDTLLRATVDGQPLTDKQIRDEVNTFIFEGHDTTTSAISFCLYLLSRHAAVQERLFDEVKGHFGRELDRCVVYSDFAALPFLNCVVREALRLYPPIPALGRCLETDLAVEGGRIPAGTNVILLLWQLLRDEMLFADPLIFRPERHLSNKATSKKGAYSNIPFSAGPRNCIGQKFAMFEVKTMIIKMLRNFILLPIGPDVEPSIKIVLRSRNGVHFGLRPRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>B5DNL7</accession>
    <accession>A0A6I8V0S2</accession>
    <name>B5DNL7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lambda-crystallin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Had1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134396.1">
        <property type="nucleotide sequence ID" value="XM_002134360.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DNL7"/>
    <dbReference type="SMR" id="B5DNL7"/>
    <dbReference type="FunCoup" id="B5DNL7">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DNL7"/>
    <dbReference type="GeneID" id="6901728"/>
    <dbReference type="KEGG" id="dpo:6901728"/>
    <dbReference type="eggNOG" id="KOG2305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_0_0_1"/>
    <dbReference type="InParanoid" id="B5DNL7"/>
    <dbReference type="OMA" id="RDNCLTH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244442">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DNL7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050104">
        <property type="term" value="F:L-gulonate 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000356">
        <property type="entry name" value="Lambda-crystallin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF1">
        <property type="entry name" value="LAMBDA-CRYSTALLIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="4">
        <location>
            <begin position="9"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="3">
        <location>
            <begin position="193"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002134396.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="35547" checksum="481027CBDA365BE2" modified="2008-10-14" version="1">MSQVKKNEKVGIVGSGLIGRSWSMLFASVGYQVMLYDILPEQVSTALTATQKELLDLEAKGLLRGKLTAKQQFSCMSGTNDIKELVKGAIFIQECIPERLDLKKALYKQLDAVVDPKTILSSSTSTFLPSLFSADLKNKQNVLVSHPVNPPYYVPLVEIVPAPWTKPEFVVKTRALMDEIGQKPVTLSREIEGFALNRIQYAILNETWRLVEAGILNVKDIDSVMSDGLGMRYAFLGPLETAHLNAEGMANYFERYSKTIYNVSETMGPTPKMEGPVAEEVSKQLNEMVPLDKLVERRTYRDNCLTHLSILKSKLNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>B5DQS7</accession>
    <accession>A0A6I8V0D3</accession>
    <name>B5DQS7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134766.1">
        <property type="nucleotide sequence ID" value="XM_002134730.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DQS7"/>
    <dbReference type="STRING" id="46245.B5DQS7"/>
    <dbReference type="GeneID" id="6900371"/>
    <dbReference type="KEGG" id="dpo:6900371"/>
    <dbReference type="eggNOG" id="ENOG502TBHZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1779385_0_0_1"/>
    <dbReference type="InParanoid" id="B5DQS7"/>
    <dbReference type="OMA" id="MNISNRF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245019">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134766.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="16066" checksum="C6B40990C1663544" modified="2008-10-14" version="1">MPMNISNRFMMLECLRENAANRRIEDQALLDRISAALVKSRESLRKIQDMNNQLKVINISVKNALASIRDPAPADVAYVVKDIYNILVDNVVASLSNPVFASVAQRLEPEMIIPLDSAPAPEEVAHPDSDPQPVAEPSASPEFSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D52</accession>
    <accession>A0A6I8UBY1</accession>
    <name>Q29D52_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein anon-73B1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4812677</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0239 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353061.1">
        <property type="nucleotide sequence ID" value="XM_001353025.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D52"/>
    <dbReference type="FunCoup" id="Q29D52">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="GeneID" id="4812677"/>
    <dbReference type="KEGG" id="dpo:4812677"/>
    <dbReference type="eggNOG" id="ENOG502S6QI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189363_0_0_1"/>
    <dbReference type="InParanoid" id="Q29D52"/>
    <dbReference type="OMA" id="FQMVCLA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077967">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29D52">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009621">
        <property type="entry name" value="UPF0239"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14409">
        <property type="entry name" value="MANNOSIDASE, BETA A, LYSOSOMAL-LIKE, MANBAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14409:SF0">
        <property type="entry name" value="PROTEIN MANBAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06783">
        <property type="entry name" value="UPF0239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353061.1"/>
        </source>
    </evidence>
    <sequence length="86" mass="9506" checksum="DCAAE3A9CC67BBED" modified="2006-04-04" version="1">MSASVESIVTGAAAAASLDKYGEEDLFSILIRYGLYVGAIFQFVCISAAVLMAGEETVDANETERESEPVRARLHKIRKLEKKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DC3</accession>
    <accession>A0A6I8UBR8</accession>
    <name>Q29DC3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Complex I-39kD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">NADH-ubiquinone oxidoreductase 39 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ND-39</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction. Interacts with BLOC1S1. Interacts with SLC2A4. Interacts with CLOCK. Interacts with RAB5IF.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I NDUFA9 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352990.1">
        <property type="nucleotide sequence ID" value="XM_001352954.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DC3"/>
    <dbReference type="SMR" id="Q29DC3"/>
    <dbReference type="FunCoup" id="Q29DC3">
        <property type="interactions" value="1812"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DC3"/>
    <dbReference type="GeneID" id="4812025"/>
    <dbReference type="KEGG" id="dpo:4812025"/>
    <dbReference type="eggNOG" id="KOG2865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_4_1"/>
    <dbReference type="InParanoid" id="Q29DC3"/>
    <dbReference type="OMA" id="VLTRNMQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079299">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DC3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05271">
        <property type="entry name" value="NDUFA9_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000537">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 39 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="6">
        <location>
            <begin position="64"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00042000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352990.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="46744" checksum="5A3EED2984015C0D" modified="2006-04-04" version="1">MAAIVLTRNMQVAKHHGSGVVGVLCLRGYAAAAEDAPRPLKTINPAAMKRGTGGRSSFNGIVATVFGATGFVGRYVCNKLGKSGTQMILPYRGEDSEVIRLKVTGDLGQVLFHFYNLEDAASIRNAVKHSNVVINLVGRDFETKNFKFKDVNVTGAERIARICREVGVERFIHLSSLNVEANPKPLYVKGGSEWLRSKYEGELRVRDAFPNATIIRPADIYGSEDRFLRYYAHIWRRQFRSMPLWHKGELTVKQPVFVTDVAQAIINAAKEPDSAGRIYQAVGPKRYQLSELVDWFHRLMRKDQKRWGYQRYDMRWDPTFLMKAKLTSFICPGAPIGGLHPERIEREGITDKVLPGVPTLEDLGVQLTTMEQQVPWELRPYRAALYYDAELGEFETPEPPKTIEAREELRLFA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DY1</accession>
    <name>KLP68_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Kinesin-like protein Klp68D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">Klp68D</name>
        <name type="ORF">GA20244</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plus-end directed microtubule motor that may be used for anterograde axonal transport and could conceivably move cargos in fly neurons different than those moved by kinesin heavy chain or other plus-end directed motors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL30282" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CH379070">
        <property type="protein sequence ID" value="EAL30282.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001352782.1">
        <property type="nucleotide sequence ID" value="XM_001352746.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043852.1">
        <property type="nucleotide sequence ID" value="XM_015188366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DY1"/>
    <dbReference type="SMR" id="Q29DY1"/>
    <dbReference type="FunCoup" id="Q29DY1">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DY1"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0287799">
        <property type="protein sequence ID" value="FBpp0286237"/>
        <property type="gene ID" value="FBgn0080239"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0377361">
        <property type="protein sequence ID" value="FBpp0338404"/>
        <property type="gene ID" value="FBgn0080239"/>
    </dbReference>
    <dbReference type="GeneID" id="4812180"/>
    <dbReference type="KEGG" id="dpo:4812180"/>
    <dbReference type="CTD" id="39332"/>
    <dbReference type="eggNOG" id="KOG4280">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_22_4_1"/>
    <dbReference type="InParanoid" id="Q29DY1"/>
    <dbReference type="OMA" id="DMIRVMK"/>
    <dbReference type="PhylomeDB" id="Q29DY1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080239">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DY1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000029">
        <property type="entry name" value="Kinesin-like protein KIF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF21">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0206">Cytoskeleton</keyword>
    <keyword id="KW-0493">Microtubule</keyword>
    <keyword id="KW-0505">Motor protein</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000270579" description="Kinesin-like protein Klp68D">
        <location>
            <begin position="1"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Kinesin motor" evidence="3">
        <location>
            <begin position="19"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="371"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="610"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="722"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="350"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="432"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="417"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="626"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="782"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P46867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EAL30282.1"/>
        </source>
    </evidence>
    <sequence length="797" mass="89225" checksum="37D6B08E3F6FA23C" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29I93</accession>
    <name>ASGL1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Probable isoaspartyl peptidase/L-asparaginase GA20639</fullName>
            <ecNumber>3.4.19.5</ecNumber>
            <ecNumber>3.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Beta-aspartyl-peptidase GA20639</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Isoaspartyl dipeptidase GA20639</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>L-asparagine amidohydrolase GA20639</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Probable isoaspartyl peptidase/L-asparaginase GA20639 alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Probable isoaspartyl peptidase/L-asparaginase GA20639 beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="ORF">GA20639</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has both L-asparaginase and beta-aspartyl peptidase activity. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of an alpha and beta chain produced by autocleavage.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Cleaved into an alpha and beta chain by autocatalysis; this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ntn-hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.5"/>
    <dbReference type="EC" id="3.5.1.1"/>
    <dbReference type="EMBL" id="CH379063">
        <property type="protein sequence ID" value="EAL32760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29I93"/>
    <dbReference type="SMR" id="Q29I93"/>
    <dbReference type="FunCoup" id="Q29I93">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29I93"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0275211">
        <property type="protein sequence ID" value="FBpp0273649"/>
        <property type="gene ID" value="FBgn0080633"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815446"/>
    <dbReference type="eggNOG" id="KOG1592">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021603_1_2_1"/>
    <dbReference type="InParanoid" id="Q29I93"/>
    <dbReference type="OMA" id="ILAAMEY"/>
    <dbReference type="PhylomeDB" id="Q29I93"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080633">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008798">
        <property type="term" value="F:beta-aspartyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033345">
        <property type="term" value="P:L-asparagine catabolic process via L-aspartate"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04702">
        <property type="entry name" value="ASRGL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000001">
        <property type="entry name" value="Isoaspartyl peptidase/L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033844">
        <property type="entry name" value="ASRGL1_meta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF41">
        <property type="entry name" value="ISOASPARTYL PEPTIDASE_L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000420569" description="Probable isoaspartyl peptidase/L-asparaginase GA20639 alpha chain">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000420570" description="Probable isoaspartyl peptidase/L-asparaginase GA20639 beta chain">
        <location>
            <begin position="184"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="212"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="235"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q7L266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="325" mass="34399" checksum="BA6B5E618AB35038" modified="2006-04-04" version="1" precursor="true">MPRPVLLIHGGAGDIPDSRIAGKFKGIKEALRCAWGSLVPASGAKGGALDAVETAVRSMELDENFNAGYGSCLNTDGQVEMEASLMEGQDLRAGCVTLLRDVMHPITVARRLMEKQRHTFIGGEAAQELALSTGSERLPANALVTEGARFTLQQFKEQLTQGKDPFFARTELAAEQKTDPSGETVGAVAMDHNGQIVVGTSTGGITGKWPGRIGDTPILGSGTYADNARGGVSTTGHGETIMRYNLAQRILAAIEHKGMSAQAAADQECREMTRRIGGTGGAIVVGHAGDLGISFTSQRMAWGYIQDDTIFYGIEGQVVHQEPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IC1</accession>
    <accession>A0A6I8UHX6</accession>
    <name>Q29IC1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kelch-like protein diablo</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4815679</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. May have a role in synapse differentiation and growth.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355673.2">
        <property type="nucleotide sequence ID" value="XM_001355637.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IC1"/>
    <dbReference type="FunCoup" id="Q29IC1">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IC1"/>
    <dbReference type="GeneID" id="4815679"/>
    <dbReference type="KEGG" id="dpo:4815679"/>
    <dbReference type="eggNOG" id="KOG4441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004253_14_2_1"/>
    <dbReference type="InParanoid" id="Q29IC1"/>
    <dbReference type="OMA" id="HFMEVTK"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074674">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IC1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18444">
        <property type="entry name" value="BACK_KLHL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18234">
        <property type="entry name" value="BTB_POZ_KLHL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF450">
        <property type="entry name" value="KELCH-LIKE PROTEIN DIABLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355673.2"/>
        </source>
    </evidence>
    <sequence length="653" mass="72262" checksum="B589119861E133D9" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29K39</accession>
    <accession>A0A6I8UJ21</accession>
    <name>Q29K39_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Parkin coregulated gene protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4816593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356276.2">
        <property type="nucleotide sequence ID" value="XM_001356240.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29K39"/>
    <dbReference type="SMR" id="Q29K39"/>
    <dbReference type="FunCoup" id="Q29K39">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29K39"/>
    <dbReference type="GeneID" id="4816593"/>
    <dbReference type="KEGG" id="dpo:4816593"/>
    <dbReference type="eggNOG" id="KOG3961">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073223_1_1_1"/>
    <dbReference type="InParanoid" id="Q29K39"/>
    <dbReference type="OMA" id="DLDYCYY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074502">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29K39">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207:SF2">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21207">
        <property type="entry name" value="PARKIN COREGULATED GENE PROTEIN PARK2 COREGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356276.2"/>
        </source>
    </evidence>
    <sequence length="264" mass="30004" checksum="AB836832B352DB6E" modified="2008-10-14" version="2">MCSNTQERATRAHEPGRVCREFLYLRSKRPNRVVPAFTYQALQKNTVVKPPPKIDIFKRRPVKETVFKIYFSRGDIPCVLAGRSSKQDPTKDRSVRWTCVPEELDYCYYLPIFVDGLADMNSDTRLLAINGAIDLIIRSPKKVLPVLPKLILPLKRAFQTRDKRIIISALQVVQLMVRLGPCVGEALVPFYRQLLAVCNIYKNINVNLGAGIDPDRSKRIGDVIEDTLKLLEYCGGPNAFINIKYMVPTYESSVFPRCEMPAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q29K50</accession>
    <accession>A0A6I8UJ38</accession>
    <name>Q29K50_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-tocopherol transfer protein-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4816363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356265.2">
        <property type="nucleotide sequence ID" value="XM_001356229.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29K50"/>
    <dbReference type="FunCoup" id="Q29K50">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="GeneID" id="4816363"/>
    <dbReference type="KEGG" id="dpo:4816363"/>
    <dbReference type="eggNOG" id="KOG1471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046597_1_1_1"/>
    <dbReference type="InParanoid" id="Q29K50"/>
    <dbReference type="OMA" id="CLPACYG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070239">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF220">
        <property type="entry name" value="LD41874P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356265.2"/>
        </source>
    </evidence>
    <sequence length="337" mass="38783" checksum="1C1FB1D8406ADEF4" modified="2008-10-14" version="2">MTMLSTAAAVRGLPQQQTSAAVALSRVAAFDSGVPTMLSDIDQLPSIQLGEYTLQFELGEPTAMGKEVAIKELRESPERQKEAVKELARLLEAETDLHYPKDNEEWLIRFLRPCKYYPESARDLIKRYYSFKVKHSDVYNNLKPSGEANIFKNNILTVFPNRDQCGRRILVLELGKRWKHKQVSLDEVFKGAVIFLEAAMLEPETQIHGAVVIFDMDGLSLQQTWQFTPPFAKRIVDWLQDSVPLRIKAIHIVNQPKMFNVVFALFKPFLREKLRGRIIFHGTDRDSLHKYMSPKCLPPAYGGILELKRVESDQWYQLLLKCDTEFDAINSYGYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KF8</accession>
    <accession>A0A6I8UIV3</accession>
    <name>Q29KF8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Interferon-related developmental regulator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Ifrd1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IFRD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356157.1">
        <property type="nucleotide sequence ID" value="XM_001356121.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KF8"/>
    <dbReference type="SMR" id="Q29KF8"/>
    <dbReference type="FunCoup" id="Q29KF8">
        <property type="interactions" value="1403"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KF8"/>
    <dbReference type="GeneID" id="4816422"/>
    <dbReference type="KEGG" id="dpo:4816422"/>
    <dbReference type="eggNOG" id="KOG2842">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031384_1_2_1"/>
    <dbReference type="InParanoid" id="Q29KF8"/>
    <dbReference type="OMA" id="QCFEAIF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076416">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039777">
        <property type="entry name" value="IFRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006921">
        <property type="entry name" value="Interferon-rel_develop_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007701">
        <property type="entry name" value="Interferon-rel_develop_reg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354:SF1">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05004">
        <property type="entry name" value="IFRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04836">
        <property type="entry name" value="IFRD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Interferon-related developmental regulator N-terminal" evidence="3">
        <location>
            <begin position="29"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon-related developmental regulator C-terminal" evidence="2">
        <location>
            <begin position="369"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356157.1"/>
        </source>
    </evidence>
    <sequence length="427" mass="47455" checksum="8D9372C513AC93F3" modified="2006-04-04" version="1">MPRRNKKSGGGQGKGRNNESNSEDESFDNVSVYSHVSEVASSEANDDLANERFEEKFEKALEQATEKSAQTRVQALQAICELLMHRYMPDFVEDRKMTLMDFVEKSIRRGKGQEQVWGARLAPLLVLQMGGDKGISKAMNQFLLTTVQDKSIGFDARAKCVTALGLLSFLGCEDVAELVRLMQYFEAIFAGSYLRGDDKTPVSVTAEAGALHAEALSAWGLLLTLIPPGDFVSLMTTGNNMFPSIKKFLGLLQSPHLDVRMAAGETIALILESGRAHDDDFLEEEIAELSEAVKQLATDSHKYRAKRDRKAQRATFRDVLRYLEEDISPEINIRFGTESLTLDSWSIHHQYSALCTVMGPGMTSQLQENEFIRDIFELGARPTNTGINGNAKIKPTKLERHLVNAAAFKARSISRGKNRDKRSAVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KW4</accession>
    <accession>A0A6I8UIG3</accession>
    <name>Q29KW4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Taf10b</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TAF10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356001.1">
        <property type="nucleotide sequence ID" value="XM_001355965.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KW4"/>
    <dbReference type="SMR" id="Q29KW4"/>
    <dbReference type="FunCoup" id="Q29KW4">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KW4"/>
    <dbReference type="GeneID" id="4816552"/>
    <dbReference type="KEGG" id="dpo:4816552"/>
    <dbReference type="eggNOG" id="KOG3423">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064104_3_0_1"/>
    <dbReference type="InParanoid" id="Q29KW4"/>
    <dbReference type="OMA" id="GFECDDV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075921">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KW4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07982">
        <property type="entry name" value="HFD_TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003923">
        <property type="entry name" value="TAF10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03540">
        <property type="entry name" value="TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017246">
        <property type="entry name" value="TFIID_TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01443">
        <property type="entry name" value="TFIID30KDSUB"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356001.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="16341" checksum="4F7B5B87CA42BF92" modified="2006-04-04" version="1">MVGSNFGIIYGLTGPSNQGASSAGQGGAGMAAGGGSADRDRLTTAAHLSDFMLQLEDYTPLIPDAVTAHYLNLGGFQADDKRIVRLISIAAQKYMSEIMDDALQHSKARTHLQTTNTPGGSKAKDRKFTLTMEDLQPALADYGINVRKMDYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q29P26</accession>
    <accession>A0A6I8ULF4</accession>
    <name>Q29P26_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase CHIP</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RING-type E3 ubiquitin transferase CHIP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">STUB1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001357398.1">
        <property type="nucleotide sequence ID" value="XM_001357362.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29P26"/>
    <dbReference type="SMR" id="Q29P26"/>
    <dbReference type="FunCoup" id="Q29P26">
        <property type="interactions" value="1533"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29P26"/>
    <dbReference type="GeneID" id="4818095"/>
    <dbReference type="KEGG" id="dpo:4818095"/>
    <dbReference type="eggNOG" id="KOG4642">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056455_1_0_1"/>
    <dbReference type="InParanoid" id="Q29P26"/>
    <dbReference type="OMA" id="WAGVEHD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078734">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29P26">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16654">
        <property type="entry name" value="RING-Ubox_CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000124">
        <property type="entry name" value="STIP1 homology and U box-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045202">
        <property type="entry name" value="CHIP_RING-Ubox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041312">
        <property type="entry name" value="CHIP_TPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18391">
        <property type="entry name" value="CHIP_TPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="9">
        <location>
            <begin position="212"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357398.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="34200" checksum="A3EDDCF801E150F9" modified="2006-04-04" version="1">MTTKHMYSTTNLSDLQLKEQGNCLFAARKYDDAINCYSKAIIKNPTNATFFTNRALCNLKLKRWELCCQDCRRALDIDANLLKGHFFLGQGLIEIDSYDEAIKHLQRAYDLSKEQRQNFGDDITLQLRLARKKRWNVLEEKRMQEEIELQSYLNRLIKDDMESRLSNLKLNENLHEDQLKEKQQEIEQECDDHIKELNNIYSKVDERRRKRDVPDFLCGKISFEILTDPVITPSGITYERKDIEEHLQRVGHFDPVTRVKLTQDQLIPNFSMKEVVDSFIAENEWSLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ11</accession>
    <accession>A0A6I8VKQ4</accession>
    <name>Q2LZ11_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">Tsp74F</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10">MV-25-SWS-2005</strain>
            <tissue evidence="9 10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353962.1">
        <property type="nucleotide sequence ID" value="XM_001353926.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043352.1">
        <property type="nucleotide sequence ID" value="XM_015187866.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ11"/>
    <dbReference type="STRING" id="46245.Q2LZ11"/>
    <dbReference type="GeneID" id="4813743"/>
    <dbReference type="KEGG" id="dpo:4813743"/>
    <dbReference type="eggNOG" id="KOG3882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055524_4_2_1"/>
    <dbReference type="OMA" id="CSLFRMI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078923">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZ11">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03165">
        <property type="entry name" value="NET-5_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000037">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF527">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015043352.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="26469" checksum="0C86A22C68DAE00B" modified="2006-02-21" version="1">MGFSSRMDCCGQFVKYSLFIANFVIFVGGAIVFCLTLWTLVDRSFVNELLGTNLFSGAVYVLLVTSVIICLVSFLGCVGAGKEVKCLLLTYFIIVALVFVTMLIGGVLGYVFRERVQQTMRQEMRSTMALYGSRREITQAWDLTQERLQCCGVDTWHDWNRYGPVPESCCQELFGGQRKECTIFPTITNLYNQGCLYVTTNFIRDHAAVIGGTSIAVAILMIFGMIFSCLLFNMIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M024</accession>
    <accession>A0A6I8UD62</accession>
    <name>Q2M024_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4813840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353599.1">
        <property type="nucleotide sequence ID" value="XM_001353563.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M024"/>
    <dbReference type="FunCoup" id="Q2M024">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M024"/>
    <dbReference type="GeneID" id="4813840"/>
    <dbReference type="KEGG" id="dpo:4813840"/>
    <dbReference type="eggNOG" id="KOG4491">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036918_3_1_1"/>
    <dbReference type="InParanoid" id="Q2M024"/>
    <dbReference type="OMA" id="MSSFACC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070189">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M024">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001353599.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="36296" checksum="F05F0095D809AE38" modified="2006-02-21" version="1">MRNWMPVLFCGLAIPISLFMWLGNVAFSKIWNNAYEEPRVIPPMRWLFSTLAPLTLMTVALRRRSVSRSGAALGILVAFILSVASHPFFASLVVFFFSSSRSTKFRAHMKRRFEHDFKEGEGQRNWVQVLCNGGMAAQLALLYLIDCGSGERAIDFSKEYRSSWLGIAVMSAFACCNGDTWSSELGSVLSQRDPISIISWRRVPRGTNGGISLIGVVVSLLGGFLVGFGYFVTVRYTVEAKMLLVSPPQWPIILFGGIAGIFGSLLDSLLGGMLQFSGINEQGQIVDAPGKGVRHVCGLRILDNHSVNLISSIITGVTIPVLALKFWPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0Q1</accession>
    <accession>A0A6I8UCK2</accession>
    <name>Q2M0Q1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Mes2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Mes2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353372.2">
        <property type="nucleotide sequence ID" value="XM_001353336.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0Q1"/>
    <dbReference type="FunCoup" id="Q2M0Q1">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="GeneID" id="4813078"/>
    <dbReference type="KEGG" id="dpo:4813078"/>
    <dbReference type="eggNOG" id="ENOG502TCFF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_674895_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M0Q1"/>
    <dbReference type="OMA" id="IALDMKP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070815">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0Q1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF69">
        <property type="entry name" value="SI:CH73-59F11.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353372.2"/>
        </source>
    </evidence>
    <sequence length="457" mass="49225" checksum="EF335E223C21462B" modified="2008-10-14" version="2">MAPVSAISGRSPSATNSLQDLSAAAAAIALDMTPKVELMPLSSSTAVAPTQKIKKLVRRNASDKLKLIQMVHDNPILWDSRLPNFKGAEEEKNRAWEHIGREFNAPGRRVARAFKSLRESYRRELAHVKMMGNGFKPKWSLYEAMDFLRDVIRERKGASHATDLSLSAYAAHLHNNNNSHNNNNHSILGGGAGGKGGAMKLSSSFNDSASALNLSKCSTLNASGDDYYCDYYVKPELDLSTGGGAGSSSSGSCSGAGGGPPALPQPAHQHQANNDSRVSSTRNENHHLQQSYDDMDASSLRSGDEDADAGNGSDGVEELEAIDADFPYPLILDSNSPGSTNAGVDEVASSRKRRRNGEQEDLDDGDADGDGGVIDGDDYEEQMLQQHRHLRQQQNAAVVTGGLDLPPPQTVREVLNSKFCGFISAKLNSMEDSEADNLMNRILLLLVQMQQCEGSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M161</accession>
    <accession>A0A6I8UCB1</accession>
    <name>Q2M161_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase-like protein 22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4812845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353212.1">
        <property type="nucleotide sequence ID" value="XM_001353176.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M161"/>
    <dbReference type="FunCoup" id="Q2M161">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M161"/>
    <dbReference type="GeneID" id="4812845"/>
    <dbReference type="KEGG" id="dpo:4812845"/>
    <dbReference type="eggNOG" id="KOG2497">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048361_1_0_1"/>
    <dbReference type="InParanoid" id="Q2M161"/>
    <dbReference type="OMA" id="EYERCPQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070472">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M161">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000313">
        <property type="entry name" value="methyltransferase-like protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038899">
        <property type="entry name" value="METTL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108:SF0">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353212.1"/>
        </source>
    </evidence>
    <sequence length="279" mass="31666" checksum="ACC418061285546C" modified="2006-02-21" version="1">MFTVTSEIYSETNYQPTKTEDGHKVVSKFRFRYPDEKLERIGRTTADKDGDLDVPRPRRGVIELEHSEATELKLVGLQVWRGALLLADYLFSQREQLANQTIMELGAGVGLTSIAAAIHSGGQVYCTDVDLGCILKLIRGNVQRNAGLLRGQVSVLEFNFLIPREEQAPELLSAIDASDVILAADVIYDDTLTDAFVTVVDQILARGRPTGRPKTIYMALEKRVVFTFEDCDSVAPMYEYFLKQTAHKPWQLEHLPLDFPQYFDYDRCRQLILMRITSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NZE8</accession>
    <accession>A0A6I8VHG5</accession>
    <name>A0A0R3NZE8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Proton-coupled amino acid transporter-like protein CG1139 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4815387</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015041474.1">
        <property type="nucleotide sequence ID" value="XM_015185988.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NZE8"/>
    <dbReference type="FunCoup" id="A0A0R3NZE8">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3NZE8"/>
    <dbReference type="GeneID" id="4815387"/>
    <dbReference type="eggNOG" id="KOG1304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3NZE8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078577">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NZE8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF150">
        <property type="entry name" value="FI17861P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="63"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015041474.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="52330" checksum="945E8691207E5DF0" modified="2016-01-20" version="1">MDAEQEQKQQQSVSEQKAIEAGEMGRTLEITPVTVTATANGDHQMEKQATDVEGSSPVKRRHATSNLEAATHLFKGSVGAGLFAMGDCFKNGGLAGATILLPIIAVMCVHCERMLIRGSVLAVERTPGATFFDYPETVEKCFEYGPRPLRRMSRIMKLIVEMFLCVTQFGFCAIYFVFITENLYQVLQQNGVDISMSMVMLITLLPAMIPSLMTNLKYISPVSAFANVALLFGLIATLSIAFSDGPMPPLGDRHLFTSGSQLSLFFGTALFSYEGIALILPLRNSMRKPENFSSRFGVLNSTMFFTTALFIFTGFVSYVRWGEDVAGSITLNLVVEDVLSQVVKVVAALGVFLGYPIQFFVMMKILWPPLKRSNSCAQKYPISMQVALRFVMVMMTFGVALVVPQLNLFISLIGALCSTCLAFVIPVLIDFVVRAQVPKGLGHWSYAKNLLILAVALLGIVTGTYQSIVEIIRQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P871</accession>
    <accession>A0A6I8VKI1</accession>
    <name>A0A0R3P871_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear receptor-binding factor 2 MIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015043276.1">
        <property type="nucleotide sequence ID" value="XM_015187790.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P871"/>
    <dbReference type="STRING" id="46245.A0A0R3P871"/>
    <dbReference type="GeneID" id="26532352"/>
    <dbReference type="KEGG" id="dpo:26532352"/>
    <dbReference type="InParanoid" id="A0A0R3P871"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271339">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P871">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039679">
        <property type="entry name" value="NRBF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14964">
        <property type="entry name" value="NUCLEAR RECEPTOR BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14964:SF2">
        <property type="entry name" value="NUCLEAR RECEPTOR-BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140361">
        <property type="entry name" value="MIT domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015043276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23284" checksum="49F81983BBB6EFDF" modified="2016-01-20" version="1">MDCAPLNLAHFHERRSERFMRNHRYEDAIKALETSLIYMQDAHKRVALPKSKEVLDTLTLDFQRKLRQIEMRKTQHGLTKVKDFVPLKEASPMLPLRQENSGGGSSSAQSVAKAIDRTVQEFDAKFTSTVTTKSSTGDCEPHTDMLSRSDPQGSGLEDQCRSSLERSEDLDMPSLAPLELPSFDYSMFSSSSAPSLASYAGMAESFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAQ3</accession>
    <name>A0A6I8UAQ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA pseudouridine synthase</fullName>
            <ecNumber evidence="6">5.4.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4812215</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001352470.4">
        <property type="nucleotide sequence ID" value="XM_001352434.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAQ3"/>
    <dbReference type="FunCoup" id="A0A6I8UAQ3">
        <property type="interactions" value="615"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812215"/>
    <dbReference type="InParanoid" id="A0A6I8UAQ3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160147">
        <property type="term" value="F:tRNA pseudouridine(38-40) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000011">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="7">
        <location>
            <begin position="176"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001352470.4"/>
        </source>
    </evidence>
    <sequence length="310" mass="35668" checksum="0A1F885F3B992DA3" modified="2020-08-12" version="1">MHRYLLNISYIGTTFRGIQKTINKLEPQSLDTNSVQGCLELALRVFHPTNEIQTVLSSRTDAGVHALHSTVHVDLARRDGQPYDTTTLTGVLNRTLDKQRLPIRVLSSQVVPDTFHCRYHAIGRTYLYRFAVARRPLFDDGKLRNKGFEAFVPVEEIDRCYFLLAPTFDIERIRNAARMFIGVHDFRTFMSVSRQKSLSRDHPMFTLRKIDEINIRPGRTLALPSNATLAAETYDYWDIEIKAKSFLYKQVRRIVGSLLSLGSDRIDEKCIYQMLTIPSKHSWDSRVVPAPACGLYLCRVHYRDTLSTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBY3</accession>
    <name>A0A6I8UBY3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar ATPase assembly integral membrane protein VMA21 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4812078</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
            <location evidence="6">COPII-coated vesicle membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the VMA21 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352987.2">
        <property type="nucleotide sequence ID" value="XM_001352951.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBY3"/>
    <dbReference type="FunCoup" id="A0A6I8UBY3">
        <property type="interactions" value="1200"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812078"/>
    <dbReference type="InParanoid" id="A0A6I8UBY3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBY3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03058">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019013">
        <property type="entry name" value="Vma21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09446">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001352987.2"/>
        </source>
    </evidence>
    <sequence length="108" mass="11700" checksum="F8072E3B4BF194AD" modified="2020-08-12" version="1">MTNKNKKTSAGIAGAAAPSKQARQQSEDSQDYSSFKTVLFYCMLIVFLPVITFFLLKAVVLDGFFEMSELKVNIFSAVGAVVALHIALGLYIYRAYFGGSGSKASKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCF6</accession>
    <name>A0A6I8UCF6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Threonine aspartase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Tasp1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Ntn-hydrolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353268.2">
        <property type="nucleotide sequence ID" value="XM_001353232.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCF6"/>
    <dbReference type="FunCoup" id="A0A6I8UCF6">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813817"/>
    <dbReference type="InParanoid" id="A0A6I8UCF6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCF6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04514">
        <property type="entry name" value="Taspase1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000006">
        <property type="entry name" value="Threonine aspartase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037464">
        <property type="entry name" value="Taspase1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF8">
        <property type="entry name" value="THREONINE ASPARTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="3">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353268.2"/>
        </source>
    </evidence>
    <sequence length="366" mass="38892" checksum="0C715F9A61442E7A" modified="2020-08-12" version="1">MAGFVAVHTGAGNCIDETKYQRVIKEACIRATDILRNGGSAVDACEAAIIRLENCGYTNAGYGSNLCMDGSVQCDAAIMNGSTLNFGACTNVSRVKNPIQLARQICEAQSNPQLLERIPPMVLAGSGAERYADEVGCAMVDDSSLMISSKAKFQFNHYKSKYDLVVSSRNNKAASEEPVPVPDASNEVELAASLDTVGAVCVDSLGNTAAGCSSGGILLKVPGRVGQAATYGAGCWATDTDDLAIATCTTGNGEYLMKTLLAREICNGAFTSDCAVTSLHKTFTQKFLDSPLLPQQQDLYAGALTLLYYPKQSSGEVMWSHTTQSFCVGYMATTQKVPKFVHSPLPTYSVPGRSCVVNGHNFHLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCL2</accession>
    <name>A0A6I8UCL2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">adenylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ak1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001353357.4">
        <property type="nucleotide sequence ID" value="XM_001353321.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCL2"/>
    <dbReference type="FunCoup" id="A0A6I8UCL2">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813057"/>
    <dbReference type="InParanoid" id="A0A6I8UCL2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCL2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000315">
        <property type="entry name" value="Adenylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006267">
        <property type="entry name" value="AK1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01360">
        <property type="entry name" value="aden_kin_iso1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001353357.4"/>
        </source>
    </evidence>
    <sequence length="228" mass="24924" checksum="5FD0BD109E8E8D29" modified="2020-08-12" version="1">MLFMCHQMAMKKEAQEKLKAEELRKAQAAAEIPIIWILGGPGCGKGTQCAKIVEKYGFTHLSSGDLLRNEVASGSDKGRQLQAVMSSGGLVSNEEVLSLLNDAVNRAKGGSKGFLIDGYPREKTQGVAFEERIGPADLALYFDCSEDTMLKRILARAAAAAVKRSDDNEETIRARLQTFKQNTSAILELYADKTLTINAERDVDDIFQEVVQAIDCVLKKKAKIAAHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDC1</accession>
    <name>A0A6I8UDC1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Trypsin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4813499</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353694.3">
        <property type="nucleotide sequence ID" value="XM_001353658.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDC1"/>
    <dbReference type="FunCoup" id="A0A6I8UDC1">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813499"/>
    <dbReference type="InParanoid" id="A0A6I8UDC1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000006">
        <property type="entry name" value="Serine proteinase stubble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="186"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353694.3"/>
        </source>
    </evidence>
    <sequence length="423" mass="45678" checksum="BFDDD380A154BD6B" modified="2020-08-12" version="1">MGLCLDINIGSEIDLLDCVVSIRTVNKGTLLISRILRRVSFGSVRFKSRQIQENMNFNPRTFLFAVCLGLGLNWSSAVYNYKAPPRGYFYPRPERLPPLPVNGSSTGTPGSGLPPGVQSDFIDDLIEGHKQQILSSVLGGASETPLDSASAAASASFSPLEGGGAKAFRVNRCASCTCGVPNVNRIVGGTQVRTNKYPWIAQIIRGTLLFCGGTLINDRYVLTAAHCVHGMDMRGVSVRLLQLDRSSTHLGVTRSVAFAHAHAGYDPVSLVHDIALLRLDQPIPLVDTIRPACLPTHWFQNFDFQKAVVAGWGLSQEGGSTSSVLQEVVVPIITNAQCRATSYRSMIVDTMLCAGYVQTGGRDACQGDSGGPLIVRDRIFRLAGVVSFGYGCAKPDAPGVYTRVSRYLDWIAVNTRDSCYCIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDL0</accession>
    <name>A0A6I8UDL0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin carboxyl-terminal hydrolase 36</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Deubiquitinating enzyme 36</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein scrawny</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquitin thioesterase 36</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquitin-specific-processing protease 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">scny</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001353862.4">
        <property type="nucleotide sequence ID" value="XM_001353826.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDL0"/>
    <dbReference type="FunCoup" id="A0A6I8UDL0">
        <property type="interactions" value="552"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813226"/>
    <dbReference type="InParanoid" id="A0A6I8UDL0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDL0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02661">
        <property type="entry name" value="Peptidase_C19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000085">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="17">
        <location>
            <begin position="171"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="94"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="504"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="689"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="769"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="968"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1054"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="528"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="592"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="637"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="657"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="778"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="806"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="821"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="841"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="867"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="918"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="968"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1090"/>
            <end position="1101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001353862.4"/>
        </source>
    </evidence>
    <sequence length="1101" mass="121208" checksum="A71056D3F181A550" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDU8</accession>
    <name>A0A6I8UDU8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">NaPi-III</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Sodium-phosphate symporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353940.2">
        <property type="nucleotide sequence ID" value="XM_001353904.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239119.1">
        <property type="nucleotide sequence ID" value="XM_033383228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDU8"/>
    <dbReference type="KEGG" id="dpo:4813902"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDU8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="525"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="644"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="603"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="611"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353940.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033239119.1"/>
        </source>
    </evidence>
    <sequence length="676" mass="72734" checksum="74C31A9F1CD77348" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDV5</accession>
    <name>A0A6I8UDV5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">trypsin</fullName>
            <ecNumber evidence="11">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4813497</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001353852.2">
        <property type="nucleotide sequence ID" value="XM_001353816.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDV5"/>
    <dbReference type="KEGG" id="dpo:4813497"/>
    <dbReference type="InParanoid" id="A0A6I8UDV5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079695">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDV5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000025">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000043">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026336333" description="trypsin" evidence="13">
        <location>
            <begin position="17"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="40"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="311"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001353852.2"/>
        </source>
    </evidence>
    <sequence length="545" mass="57501" checksum="F80DEDD0DEDAF4D9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UED2</accession>
    <name>A0A6I8UED2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3 beta-hydroxysteroid dehydrogenase/Delta 5--&gt;4-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4813984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354139.3">
        <property type="nucleotide sequence ID" value="XM_001354103.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UED2"/>
    <dbReference type="FunCoup" id="A0A6I8UED2">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813984"/>
    <dbReference type="InParanoid" id="A0A6I8UED2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000495">
        <property type="entry name" value="3 hydroxysteroid dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF853">
        <property type="entry name" value="GH25466P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354139.3"/>
        </source>
    </evidence>
    <sequence length="409" mass="45562" checksum="6D2DCE92288F4400" modified="2020-08-12" version="1">MSSSNSSNSGSGGAGDVVLVTGGSGFLGQHLIKQLLERRKELGIREVRSLDIVPYKNNIGHPETSILRTFVGDIGVDRESLGPVFAGVDGVFHCAASVKIEYPPNYEELDRVNVKGTLAIVDLCIQNNVKRLVYTSCTSVCFVPFKGRSTFSAVINSTESKTDTPTLDGASLWEQDSQFLIAGYASSKLRGENIVLNSNGAPLQNQQDYLATCAIRAPLTYGECDSHFVTNTFEYLSTRGWVFPRIAGVGGKQQLVYAGNVAWGHLCAYKALKVSSKAVNGLPVFVTDDTGINDVSRFIQKMALLGERFKVKTSWWYVPHFLFFFLAFLLEVVVRIAYPYTKYRLHYSPRAIASYTSSMLMYNRLRAAIHMDYMPLFDADASAERSAKWYARWWDQKQQARQQQPKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF41</accession>
    <name>A0A6I8UF41_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein vnd isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">vnd</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NK-2 homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354499.3">
        <property type="nucleotide sequence ID" value="XM_001354463.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UF41"/>
    <dbReference type="FunCoup" id="A0A6I8UF41">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814937"/>
    <dbReference type="InParanoid" id="A0A6I8UF41"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079405">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000101">
        <property type="entry name" value="NK2 homeobox 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF82">
        <property type="entry name" value="HOMEOBOX PROTEIN VND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="560"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="562"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="249"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="475"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="72"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="275"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="527"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001354499.3"/>
        </source>
    </evidence>
    <sequence length="740" mass="77941" checksum="D1F4DABE54455DA3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFQ5</accession>
    <name>A0A6I8UFQ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4815053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354769.2">
        <property type="nucleotide sequence ID" value="XM_001354733.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFQ5"/>
    <dbReference type="FunCoup" id="A0A6I8UFQ5">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815053"/>
    <dbReference type="InParanoid" id="A0A6I8UFQ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFQ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001354769.2"/>
        </source>
    </evidence>
    <sequence length="312" mass="34501" checksum="323D3075CDC7661C" modified="2020-08-12" version="1">MESNQNPSLHQAIQQKLGLQNDSESTKLNTSAFKLIIPQLKIPQLNKGNPTPIDLAEQEKERQAVDARNKLLLNEMKRRRQISEGEKTAGQPFSGFVIPQLCVAPGEPSADAAKLNIPLLNKLLSQNKSSGPLPKLERAVDRLHISGGDGGDTIPPVHPVPTAAPLIDLTTAVIKGNQNAPPKEYASKARLKRAQNTHTFDIPFIAYDPVKRFAPSICLLTDDKCSMSVDGSDLLAPEHHSVIGSLLDSVVGYPEPRKPQVKYAISLLEKQHLKMYRREDYGSNVKRFRFDTPSPDEVVKQALQKTWRSSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH51</accession>
    <name>A0A6I8UH51_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">AMPKalpha</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001355447.2">
        <property type="nucleotide sequence ID" value="XM_001355411.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UH51"/>
    <dbReference type="FunCoup" id="A0A6I8UH51">
        <property type="interactions" value="1803"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815770"/>
    <dbReference type="InParanoid" id="A0A6I8UH51"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004679">
        <property type="term" value="F:AMP-activated protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043050">
        <property type="term" value="P:nematode pharyngeal pumping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14336">
        <property type="entry name" value="UBA_AID_AMPKalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000079">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000055">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000136">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000014">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032270">
        <property type="entry name" value="AMPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049020">
        <property type="entry name" value="PRKAA1/2_AID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16579">
        <property type="entry name" value="AdenylateSensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21147">
        <property type="entry name" value="AMPK_alpha_AID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="28"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001355447.2"/>
        </source>
    </evidence>
    <sequence length="587" mass="65047" checksum="3A40CD1DC706D247" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIP4</accession>
    <name>A0A6I8UIP4_DROPS</name>
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        <recommendedName>
            <fullName evidence="9">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4816453</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the exonuclease superfamily. TREX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356147.2">
        <property type="nucleotide sequence ID" value="XM_001356111.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIP4"/>
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        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816453"/>
    <dbReference type="InParanoid" id="A0A6I8UIP4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIP4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040393">
        <property type="entry name" value="TREX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13058:SF19">
        <property type="entry name" value="LD40940P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13058">
        <property type="entry name" value="THREE PRIME REPAIR EXONUCLEASE 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="9">
        <location>
            <begin position="20"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001356147.2"/>
        </source>
    </evidence>
    <sequence length="335" mass="37938" checksum="096B8E13516BFE0A" modified="2020-08-12" version="1">MAPSNSNDGEQYEDQHKISTFAVLDLETTNLPANNNNRVGITELCIYAFEAALLKQNSTKGSGENKVPVPPLPRVVHKLNVLFQPSMMVHPDAERFTGLNNYILERESKLDENSAQMILNFLKHLPSPVCLVAHNGWHFDFPIVRKVFAKLNLEFSPTLSCVDSLRAFLEIDDKQSREDGLMKLPQSLSVKVEEAEEAEPIKEPDWRMLNETTPKRPILTPTEAASKRRQISESEDDELEASSPVKRTLSSRRQLFSGLQCAKKKRWPPRGKYNLGSLYERKFNQPPTNAHQAEADVAMVTALIQHYGMDFRAFAEEQAIPFSQIIPLGSPDRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJ68</accession>
    <name>A0A6I8UJ68_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tenascin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NimB1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356222.2">
        <property type="nucleotide sequence ID" value="XM_001356186.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJ68"/>
    <dbReference type="FunCoup" id="A0A6I8UJ68">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816639"/>
    <dbReference type="InParanoid" id="A0A6I8UJ68"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000961">
        <property type="entry name" value="GD23942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000701">
        <property type="entry name" value="tenascin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053255">
        <property type="entry name" value="EGF-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24047">
        <property type="entry name" value="FI01909P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24047:SF32">
        <property type="entry name" value="FI01909P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026076601" evidence="1">
        <location>
            <begin position="28"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="112"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="149"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="218"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="286"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="338"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356222.2"/>
        </source>
    </evidence>
    <sequence length="408" mass="45188" checksum="75ACFF4358C000D5" modified="2020-08-12" version="1">MHSNGLCTLSPVMKGLLMLAAVMEMLAMIGAMPEEESQQLFGDIHITSDMESLSLTRERQKHLCQREVPVVFFQTQKDSPPRGNGSTIFYHRIDVCCLGYRRNPYSGRCVPDCSQSSPDNCFNGFCRAPGQCECFAEFVRNAHGACIHTCPISCQDGRCFLNGTCACHPGYALDRETRLFCRPHCSQDCGTHEECVAPGMCDCSPGYRRTDELGCQPICTPDCGYGKCVGPNQCECFPGFIKRPQRSVCEAECHINCENGFCESRYKCQCREGYRYDLNTTSCLPECVDDCGHGNGVCIAPGVCRCFEGHEGRGNGCHPKCDGSCGPHGLCLKPNICGCQPSQQHCLNGSCDANGHCHCPNGQTHFVDRCLRPQQLIHQLVTNERRVHFNLQLGYEFNALIGRHFNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK42</accession>
    <name>A0A6I8UK42_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RCC1-like G exchanging factor-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4817025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356744.3">
        <property type="nucleotide sequence ID" value="XM_001356708.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK42"/>
    <dbReference type="FunCoup" id="A0A6I8UK42">
        <property type="interactions" value="700"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817025"/>
    <dbReference type="InParanoid" id="A0A6I8UK42"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053035">
        <property type="entry name" value="Mitochondrial_GEF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46337">
        <property type="entry name" value="RCC1-LIKE G EXCHANGING FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46337:SF1">
        <property type="entry name" value="RCC1-LIKE G EXCHANGING FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="48"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="112"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="180"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="221"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="294"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="349"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="407"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356744.3"/>
        </source>
    </evidence>
    <sequence length="458" mass="50207" checksum="F1B77CF6E9C1AF98" modified="2020-08-12" version="1">MLSTVRVGAWTTPRLTRTYTAKAKRSVLSQDTTKIKDYTPKVSDPHAHRVYVWGFQETGALGLQTSVKKAQERYTEMVHHPTRLQFSNNNEITDVAAGYGFTAYAVQRSDGETLFGSGLNTDSQLGFQVKGNPKDPANLDVIIYPTAIRLPREHDETDEDMQVRSMAAGRAHLVVVTRNGTVFTLGNNSYGQCGRSIIDDERYSKSALIHRIAQADICGEKDEIVSVECGQDHTMFLTKLGRVYTCGWGADGQTGQGNYHTAGQITLIGGDIEKERIVRLTSSSDCVLALNERGDVFGWGNSEYGQLDDSEHAQTQINTPRALQLTKGIGLIKDVAAGGSFCMALNDQGLVYTWGYGILGFGPFVEQTSKPQHLLPPLFGRNDFSNATTVVSIGCGVFHMGAVNSDGDLFMWGKNRFGHLGLGHKKDQFFPFKAAINGKVTKVAYGVDHTIALCKPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKP4</accession>
    <name>A0A6I8UKP4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b5 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4817588</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Radial spoke stalk protein that binds heme under oxidizing conditions. Required for the coordinated beating of multiple cilia maybe by functioning in a redox signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356991.2">
        <property type="nucleotide sequence ID" value="XM_001356955.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKP4"/>
    <dbReference type="SMR" id="A0A6I8UKP4"/>
    <dbReference type="FunCoup" id="A0A6I8UKP4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817588"/>
    <dbReference type="InParanoid" id="A0A6I8UKP4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052320">
        <property type="entry name" value="Cytochrome_b5_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21281">
        <property type="entry name" value="CYTOCHROME B5 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21281:SF0">
        <property type="entry name" value="CYTOCHROME B5 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="11">
        <location>
            <begin position="8"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001356991.2"/>
        </source>
    </evidence>
    <sequence length="215" mass="25189" checksum="7152A4A4D52DB60D" modified="2020-08-12" version="1">MEQPINKMRYYLKDETVLHNKKDDCWIIIHNNVYDLTPMIRDRLDHWNTNMDYLVAHAGKDLTHFFHENGEPRTEISVRTGRPRVLFPPILEVAISEFAKTNGAMWSQDPFYHIGRLTRRARLIRIINALTAQTLFMTVCDEDTIYDIQQKYKQHYNHHAGSYEWRKFSNGGKACGQLDLNGTLDENGLIDEEDCDVELPPPSIWLYYTDDITIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKQ8</accession>
    <name>A0A6I8UKQ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">RNA-binding protein 5-B-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">LOC4817616</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15 16">MV-25-SWS-2005</strain>
            <tissue evidence="15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357016.2">
        <property type="nucleotide sequence ID" value="XM_001356980.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036376.2">
        <property type="nucleotide sequence ID" value="XM_015180890.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKQ8"/>
    <dbReference type="KEGG" id="dpo:4817616"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16162">
        <property type="entry name" value="OCRE_RBM5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12313">
        <property type="entry name" value="RRM1_RRM2_RBM5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000823">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041591">
        <property type="entry name" value="OCRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948:SF3">
        <property type="entry name" value="FI21118P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17780">
        <property type="entry name" value="OCRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="216"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="13">
        <location>
            <begin position="298"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="12">
        <location>
            <begin position="909"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="653"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="942"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="95"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="661"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001357016.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015036376.2"/>
        </source>
    </evidence>
    <sequence length="978" mass="109144" checksum="DF17D0016E22A7B3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL21</accession>
    <name>A0A6I8UL21_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 138</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4818048</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM138 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357211.2">
        <property type="nucleotide sequence ID" value="XM_001357175.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL21"/>
    <dbReference type="FunCoup" id="A0A6I8UL21">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4818048"/>
    <dbReference type="InParanoid" id="A0A6I8UL21"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024133">
        <property type="entry name" value="TM_138"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13306">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13306:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14935">
        <property type="entry name" value="TMEM138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001357211.2"/>
        </source>
    </evidence>
    <sequence length="166" mass="19097" checksum="29C3EE9FBC33F82B" modified="2020-08-12" version="1">MKLTRGRYTYLLMSQMALLAGDLFFNAFGASLARNHLQTSIFFFVTQDALIIVEYLLFTLALHSTCVYQVGASHIILRNCKLFLGSITIYFLLSASQHFWIVYQSRLSAGENQNQEWPLGLYALAVIHRIMSVFYYYSSKSTALTMADPRYKEEHLDWIAEQLGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULC2</accession>
    <name>A0A6I8ULC2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">THG</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001357358.3">
        <property type="nucleotide sequence ID" value="XM_001357322.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULC2"/>
    <dbReference type="FunCoup" id="A0A6I8ULC2">
        <property type="interactions" value="1577"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817877"/>
    <dbReference type="InParanoid" id="A0A6I8ULC2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3000:FF:000005">
        <property type="entry name" value="tRNA(His) guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF6">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="13">
        <location>
            <begin position="32"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="14">
        <location>
            <begin position="163"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001357358.3"/>
        </source>
    </evidence>
    <sequence length="319" mass="37021" checksum="3EE2596251CE1957" modified="2020-08-12" version="1">MRFLGLVNKLNSLLIKTTARELRKRSMACSRYEYVKTYEQDDTILPNVWIVIRIDGKKFHKFSKTHNFEKPNDENALNVMNAAATAVMQEFRDVVLAYGQSDEYSFVFRKETAAFKRRSAKLLTYVTSLFSTSYVMQWSKWMSLPLAYAPCFDGRVVLYPSDENLRDYLSWRQADVHVNNLYNTAFWKLVLDSGLSNQKAEERLRGTFSADKNELLFQEFGINYNNLPAMYRKGTILLRKRVISDGDNDQKGRQAIVPLHEDLISSHFWKVHTEILGKYVPGTYTAPEIMPRLVDLQLNTKHEKGDYEKKQPENLAGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMA2</accession>
    <name>A0A6I8UMA2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamic acid-rich protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4800392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357677.5">
        <property type="nucleotide sequence ID" value="XM_001357640.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMA2"/>
    <dbReference type="FunCoup" id="A0A6I8UMA2">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800392"/>
    <dbReference type="InParanoid" id="A0A6I8UMA2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357677.5"/>
        </source>
    </evidence>
    <sequence length="226" mass="26195" checksum="E7AA5BF8217EBF4F" modified="2020-08-12" version="1">MEEAANGTPQSARRPRLGLMRRACHTTPLLRLQREELGTTTPTDRTEQETPRALPLSTNSRKRIGLSRIRTELTKKRLEFAAASQLKEKTSEKESTPREKEKKPQAKKAERTVASKLQEDERGKIVEDKDRLEEDDERAKIVEDKEQMEEEDEEVVDKEQMEEEDDKVEPQDSRIAELQADIETWRRGFIATMNDLQAMVDPRPTKKAVLIQLGIPLEMLRYLEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN31</accession>
    <name>A0A6I8UN31_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL35m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L35, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mRpL35</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358167.3">
        <property type="nucleotide sequence ID" value="XM_001358130.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN31"/>
    <dbReference type="FunCoup" id="A0A6I8UN31">
        <property type="interactions" value="1026"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800992"/>
    <dbReference type="InParanoid" id="A0A6I8UN31"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_bL35-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_bL35_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019338">
        <property type="entry name" value="Ribosomal_bL35m"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15909">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L35, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15909:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL35M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="L35p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001358167.3"/>
        </source>
    </evidence>
    <sequence length="175" mass="20195" checksum="BBAB31AC5A66D498" modified="2020-08-12" version="1">MLRTLLTSALRGVCRVPPVAPLVQQTQRACFGQLMTIQKQPSLLSAHTLAPASLQLLQCPALGTPGTPPVRSVTKFSLIKGKRKTVKAVLKRFKRLDWGAWIRTHSGRQKKLFKKSNALRRRLRQHVFTNASQSWLLDKMVTDYWRRPKHYIDDPYAPYHKRDEYFATKSKPFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNE9</accession>
    <name>A0A6I8UNE9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial-processing peptidase subunit beta</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">UQCR-C1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001358232.1">
        <property type="nucleotide sequence ID" value="XM_001358195.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNE9"/>
    <dbReference type="FunCoup" id="A0A6I8UNE9">
        <property type="interactions" value="2469"/>
    </dbReference>
    <dbReference type="GeneID" id="4801071"/>
    <dbReference type="KEGG" id="dpo:4801071"/>
    <dbReference type="InParanoid" id="A0A6I8UNE9"/>
    <dbReference type="OMA" id="IDVVCDM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077658">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="51"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="204"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001358232.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="52144" checksum="3A54ABD2258EE45D" modified="2020-08-12" version="1">MALRVLGVTQNMRSFLRGVDLIKRSKSTATLQKTLLNIPATQVTKLDNGLRVASEDSGAATATVGLWIDAGSRSENERNNGVAHFLEHMAFKGTAKRSQTDLELEVENLGAHLNAYTSREQTVFYAKCLSKDVPKAVEILADIIQNSKLEEEKIARERSVILREMQEIESNLQEVVFDHLHATAYQGTPLGQTILGPTKNIQSIGKSDLTDYIQTHYKASRIVLAAAGGVKHEDLVQLAGSSLGRLEASTLPPEITPCRFTGSEVRVRDDSLPLAHVAVAVEGCGWTDQDNIPLMVANTLVGAWDRSQGGGANNASNLARASAEDNLCHSFQSFNTCYKDTGLWGIYFVCDPLQCEDMIFNIQTEWMRLCTMVTEAEVERAKNLLKTNMLLQLDGTTPICEDIGRQILCYNRRIPLHELEQRINNVSVSNVRDVAMKYIYDRCPAVAAVGPVENLPDYNRIRSSMYWLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQH8</accession>
    <name>A0A6I8UQH8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional adapter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4802237</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359199.4">
        <property type="nucleotide sequence ID" value="XM_001359162.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQH8"/>
    <dbReference type="FunCoup" id="A0A6I8UQH8">
        <property type="interactions" value="597"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802237"/>
    <dbReference type="InParanoid" id="A0A6I8UQH8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UQH8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000752">
        <property type="entry name" value="Transcriptional adapter 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF20">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25299">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
        <property type="entry name" value="Transcriptional_adaptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="11">
        <location>
            <begin position="82"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="145"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="12">
        <location>
            <begin position="440"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001359199.4"/>
        </source>
    </evidence>
    <sequence length="531" mass="61730" checksum="6D3A6C09D41B5CAE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQJ3</accession>
    <name>A0A6I8UQJ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dromyosuppressin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Ms</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359137.1">
        <property type="nucleotide sequence ID" value="XM_001359100.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQJ3"/>
    <dbReference type="FunCoup" id="A0A6I8UQJ3">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="GeneID" id="4802164"/>
    <dbReference type="KEGG" id="dpo:4802164"/>
    <dbReference type="InParanoid" id="A0A6I8UQJ3"/>
    <dbReference type="OMA" id="HIKKVCM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079591">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026028559" evidence="1">
        <location>
            <begin position="25"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359137.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="10975" checksum="15CC66E4F87C5A4E" modified="2020-08-12" version="1">MSFAQFFVACCLAIVLAAVANTQAATQGPPLCQAGIVQEMPQHIQKVCMALENSDQLTSALKSYINNEASALVANSDDLLKNYNKRTDVDHVFLRFGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQV7</accession>
    <name>A0A6I8UQV7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">SWI/SNF complex subunit SMARCC1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mor</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMARCC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359275.4">
        <property type="nucleotide sequence ID" value="XM_001359238.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQV7"/>
    <dbReference type="FunCoup" id="A0A6I8UQV7">
        <property type="interactions" value="2596"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802338"/>
    <dbReference type="InParanoid" id="A0A6I8UQV7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075085">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048858">
        <property type="term" value="P:cell projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="PANTHER" id="PTHR10677:SF25">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="357"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359588.2"/>
        </source>
    </evidence>
    <sequence length="399" mass="43678" checksum="0FADD7F883A845EE" modified="2020-08-12" version="1">MHTVYALLKNKLSDVHPLHNIDLEQNVAYLKTIVTKELQLQYDASELEICHHGQVLDDVQTLSKAIKPNAVIHCFRKTTRYTPYVPPAASEINTKLIQELFSLTSHLQVSVSCRFNILQTILAEYPEFRRNLGAQALIRDSVLFNMLHEPEVVQNLVREYPLICDAAPFIVETIRKELARNSSATQFQEQAASESTTSSEEENSLGAGSSSSGSSGMVAAAANRRDELANIRQISRQQLANALANVTSFNSLSNIAQRNADEAQQGDERPRTTSAPIAGSSSSNSALGASGSSSISSELFRNELARAFQSLAQQPAESMEVEASAGAEASTDDVDDDDDAAGGDDSDVLPRCYRRHRFTEQLRTMAAMGFINHTQNVNYLTMADGNVEHAINLLMLGMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URX1</accession>
    <name>A0A6I8URX1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Mst85C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Mst85C</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359828.1">
        <property type="nucleotide sequence ID" value="XM_001359791.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URX1"/>
    <dbReference type="FunCoup" id="A0A6I8URX1">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="4803026"/>
    <dbReference type="KEGG" id="dpo:4803026"/>
    <dbReference type="InParanoid" id="A0A6I8URX1"/>
    <dbReference type="OMA" id="GIMEFGF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071372">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001359828.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="26365" checksum="15F5C2990EB97BA4" modified="2020-08-12" version="1">MNSQSKRKSFPFNPPQPGVAMQFGFNANTNFNIETECSKWPQHKRKSEQQETVLPPKMLITEERITQHFSGLQISGDSKGVVGNCAGNNNSASIVTGGLATDDIPSTSTGKTHHHPNPAYQMAAMELEQKLRNANRIVICDELKPGSVPGPLAPGVIPREWLIKSVPRPCTALVPWQPSPLQIPMPVPKLPSPMHLLAPTPPLPGEELDDYDDDLEFFENNNTCNVNLNKSDEHMDEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URX5</accession>
    <name>A0A6I8URX5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aryl hydrocarbon receptor nuclear translocator homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tgo</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359833.1">
        <property type="nucleotide sequence ID" value="XM_001359796.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URX5"/>
    <dbReference type="SMR" id="A0A6I8URX5"/>
    <dbReference type="FunCoup" id="A0A6I8URX5">
        <property type="interactions" value="1277"/>
    </dbReference>
    <dbReference type="GeneID" id="4803031"/>
    <dbReference type="KEGG" id="dpo:4803031"/>
    <dbReference type="InParanoid" id="A0A6I8URX5"/>
    <dbReference type="OMA" id="NINMFNT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071367">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18947">
        <property type="entry name" value="bHLH-PAS_ARNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000003">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000011">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050933">
        <property type="entry name" value="Circadian_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001067">
        <property type="entry name" value="Nuc_translocat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23042">
        <property type="entry name" value="CIRCADIAN PROTEIN CLOCK/ARNT/BMAL/PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00785">
        <property type="entry name" value="NCTRNSLOCATR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="13"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="85"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="292"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="449"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="530"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001359833.1"/>
        </source>
    </evidence>
    <sequence length="646" mass="71728" checksum="353344206ECE3C77" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USH7</accession>
    <name>A0A6I8USH7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sodium-independent sulfate anion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4803314</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360078.2">
        <property type="nucleotide sequence ID" value="XM_001360041.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USH7"/>
    <dbReference type="FunCoup" id="A0A6I8USH7">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803314"/>
    <dbReference type="InParanoid" id="A0A6I8USH7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="7">
        <location>
            <begin position="63"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360078.2"/>
        </source>
    </evidence>
    <sequence length="625" mass="69543" checksum="6E090A4B0356EB29" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USI3</accession>
    <name>A0A6I8USI3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WD repeat-containing protein 47</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4803523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360236.2">
        <property type="nucleotide sequence ID" value="XM_001360199.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USI3"/>
    <dbReference type="FunCoup" id="A0A6I8USI3">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="GeneID" id="4803523"/>
    <dbReference type="KEGG" id="dpo:4803523"/>
    <dbReference type="InParanoid" id="A0A6I8USI3"/>
    <dbReference type="OMA" id="EIRCIRF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075487">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040067">
        <property type="entry name" value="WDR47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19863">
        <property type="entry name" value="NEMITIN (NEURONAL ENRICHED MAP INTERACTING PROTEIN) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19863:SF5">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="176"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="266"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="312"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360236.2"/>
        </source>
    </evidence>
    <sequence length="348" mass="38848" checksum="EAF522AE6E83F00E" modified="2020-08-12" version="1">MNSEAKDLDLRKGDAERPHFEAVTTLCDSQAIRSVDFHPNGKLYAVGSNSKTFRICQYPQLSKLRNGQQTTVYPPSVLCKRTKHHRGSIYCTCWSRDGEIIATGSNDKTIKYMRFNNDTNQLVGHEMELNIHDGTVRDMCFLDDSSTKSRLLVSGGAGDCKIYITDCVSGTPFQAFSGHSGHILSLFSWNNAMFVSGSQDQTIRFWDLRVNVSVNTLEHERKDNTLGNSAVTAVCVDPTGRLLVSGHADSSCVLYDIRGNRPIQRFYPHSAEIRCVRFSPSAYYMLTCSYDNSVKLTDLQGDLAHELSSVVVAEHKDKAITIRWHPTEFSFISTSADKTATLWALPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USM9</accession>
    <name>A0A6I8USM9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">heparosan-N-sulfate-glucuronate 5-epimerase</fullName>
            <ecNumber evidence="6">5.1.3.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">Hsepi</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17 18">MV-25-SWS-2005</strain>
            <strain evidence="16">MV2-25</strain>
            <tissue evidence="17 18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[heparosan-N-sulfate](n) = [heparan-N-sulfate](n)</text>
            <dbReference type="Rhea" id="RHEA:20197"/>
            <dbReference type="Rhea" id="RHEA-COMP:9556"/>
            <dbReference type="Rhea" id="RHEA-COMP:9557"/>
            <dbReference type="ChEBI" id="CHEBI:58041"/>
            <dbReference type="ChEBI" id="CHEBI:58287"/>
            <dbReference type="EC" id="5.1.3.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; heparin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endomembrane system</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the D-glucuronyl C5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.17" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001360137.1">
        <property type="nucleotide sequence ID" value="XM_001360100.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039051.1">
        <property type="nucleotide sequence ID" value="XM_015183565.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USM9"/>
    <dbReference type="GeneID" id="4803422"/>
    <dbReference type="KEGG" id="dpo:4803422"/>
    <dbReference type="OMA" id="RGVFMYF"/>
    <dbReference type="UniPathway" id="UPA00862"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076596">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047464">
        <property type="term" value="F:heparosan-N-sulfate-glucuronate 5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030210">
        <property type="term" value="P:heparin proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010598">
        <property type="entry name" value="C5-epim_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039721">
        <property type="entry name" value="C5-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059154">
        <property type="entry name" value="Glce_b_sandwich"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13174">
        <property type="entry name" value="D-GLUCURONYL C5-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13174:SF3">
        <property type="entry name" value="D-GLUCURONYL C5-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06662">
        <property type="entry name" value="C5-epim_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21174">
        <property type="entry name" value="Glce_b_sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="D-glucuronyl C5-epimerase beta-sandwich" evidence="15">
        <location>
            <begin position="264"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="D-glucuronyl C5-epimerase C-terminal" evidence="14">
        <location>
            <begin position="417"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001360137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015039051.1"/>
        </source>
    </evidence>
    <sequence length="619" mass="69927" checksum="F0ED4EA9F83C2E9B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTT8</accession>
    <name>A0A6I8UTT8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Mdr49</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001360667.2">
        <property type="nucleotide sequence ID" value="XM_001360630.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UTT8">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="4804040"/>
    <dbReference type="KEGG" id="dpo:4804040"/>
    <dbReference type="InParanoid" id="A0A6I8UTT8"/>
    <dbReference type="OMA" id="VNGWIQM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077756">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097254">
        <property type="term" value="P:renal tubular secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017085">
        <property type="term" value="P:response to insecticide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000009">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000479">
        <property type="entry name" value="Multidrug resistance protein 1A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000214">
        <property type="entry name" value="Multidrug resistance protein homolog 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF27">
        <property type="entry name" value="ATP-DEPENDENT TRANSLOCASE ABCB1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="53"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="407"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="734"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1057"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="647"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="667"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="696"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001360667.2"/>
        </source>
    </evidence>
    <sequence length="1300" mass="142634" checksum="0E2E26FAA913D955" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU38</accession>
    <name>A0A6I8UU38_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CSN4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360654.2">
        <property type="nucleotide sequence ID" value="XM_001360617.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU38"/>
    <dbReference type="SMR" id="A0A6I8UU38"/>
    <dbReference type="FunCoup" id="A0A6I8UU38">
        <property type="interactions" value="2675"/>
    </dbReference>
    <dbReference type="GeneID" id="4804024"/>
    <dbReference type="KEGG" id="dpo:4804024"/>
    <dbReference type="InParanoid" id="A0A6I8UU38"/>
    <dbReference type="OMA" id="KNIMHTV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081270">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000130">
        <property type="entry name" value="COP9 signalosome complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041406">
        <property type="entry name" value="CSN4_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF2">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18420">
        <property type="entry name" value="CSN4_RPN5_eIF3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="200"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360654.2"/>
        </source>
    </evidence>
    <sequence length="403" mass="46059" checksum="D7206A550C4F4975" modified="2020-08-12" version="1">MGISTAILRNQLMSLINFTGTHKDQADKYRQLLRTVLSNTGQELIDALRLFVEAIVNEHVSLVISRQILNDVGVELSKLPDDLSKQLSHFTLEKVHPRVISFEEQVAGIRFHLANIYERNQQWRAAATVLVGIPLETGQKQYSVECKLGTYLKIARLYLEDNDSVQAELFINRASLLQAETNSEELQVLYKVCYARVLDYRRKFIEAAQRYNELSYRKIVDQGERMTALKKALICTVLASAGQQRSRMLATLFKDERCQHLPAYGILEKMYLERIIRRSELQEFEALLQEHQKAATPDGSSILDRAVFEHNLLSASKLYNNITFEELGALLDIPAAKAEKIASQMITEGRMNGHIDQISGIVHFENRELLPQWDRQIQSLCYQVNSIIEKIGVAEPGWLDKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWA5</accession>
    <name>A0A6I8UWA5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Augmin complex subunit dgt3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">dgt3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS3 family.</text>
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    <dbReference type="RefSeq" id="XP_001361900.2">
        <property type="nucleotide sequence ID" value="XM_001361863.5"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8UWA5"/>
    <dbReference type="FunCoup" id="A0A6I8UWA5">
        <property type="interactions" value="66"/>
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    <dbReference type="GeneID" id="4805508"/>
    <dbReference type="KEGG" id="dpo:4805508"/>
    <dbReference type="InParanoid" id="A0A6I8UWA5"/>
    <dbReference type="OMA" id="QWILYDE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="Bgee" id="FBgn0076773">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="HAUS3_N"/>
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        <property type="entry name" value="HAUS-augmin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="22"/>
            <end position="251"/>
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        <location>
            <begin position="101"/>
            <end position="139"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009645"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF14932"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001361900.2"/>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Ephydroidea</taxon>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="12">MV-25-SWS-2005</strain>
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    </reference>
    <comment type="function">
        <text evidence="8 9">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
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    <comment type="subunit">
        <text evidence="9">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
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        <text evidence="1 9">Belongs to the GPN-loop GTPase family.</text>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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            <dbReference type="RefSeq" id="XP_002132790.2"/>
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    </evidence>
    <sequence length="388" mass="43549" checksum="9F283FF774441391" modified="2020-08-12" version="1">MAAKPASSELSMENISLEALSLSEGIQDSPVCIIVLGMAGSGKTTFTRSLIQHAQEKFNPYVVNLDPACREVPYATHIDIRDTVNYKEVMKQYQLGPNGGIVTALNMFTTKMAKFAELVRRAGQRGHKWCIIDTPGQIEVFTWSASGNIITEGLATMFPTIIVYVMDVVRSACPTTFMSNMLYACSILYKTRLPFLVALNKIDLKDCSFVQEWMTDFEAYQDALEQEQSFVNNLTRTMSLTLDTFYENLVTCGVSAKTGVGFAQLIKHVLDCVTEYEKDYKPVYEKMRKERLAEQQATLQPPSTVEDSGTAVPMGLDLKDPPPYSGNNMFLMAPGLVPQQMDSEEFEDDMETDAKGNTEEQNFQTFVQSHLTAQKTKRNKFIQEQQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXX2</accession>
    <name>A0A6I8UXX2_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Titin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903209</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132910.2"/>
        </source>
    </evidence>
    <sequence length="1857" mass="216191" checksum="7F7E5C3C9F27E8CD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY50</accession>
    <name>A0A6I8UY50_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Prosbeta4R1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133075.2">
        <property type="nucleotide sequence ID" value="XM_002133039.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY50"/>
    <dbReference type="FunCoup" id="A0A6I8UY50">
        <property type="interactions" value="1314"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902535"/>
    <dbReference type="InParanoid" id="A0A6I8UY50"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 6 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002133075.2"/>
        </source>
    </evidence>
    <sequence length="210" mass="23607" checksum="CAE4228A471BE9C3" modified="2020-08-12" version="1">MALETILGIKGPDFVMLASETMQAKSLIFMKDDQSKIHRLSDFSMIATVGDGGDTLQFTDFISKNLHLYKIANGYHLSPRATAHFTRKNLADYIRTNTRYQVAMLLAGYDAVEGPDLHYIDSYGAAQSINHAGHGWGSIFCGSILQRFWDQKLTQDQAVSILKKCVAEIQKRLIINQRNWDVFVVDGSGMRQLESINPASLNYDVLSLNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYF8</accession>
    <name>A0A6I8UYF8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">LOC6903138</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132845.2">
        <property type="nucleotide sequence ID" value="XM_002132809.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036312.2">
        <property type="nucleotide sequence ID" value="XM_015180826.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYF8"/>
    <dbReference type="KEGG" id="dpo:6903138"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UYF8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="545"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="597"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002132845.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015036312.2"/>
        </source>
    </evidence>
    <sequence length="623" mass="71571" checksum="598C00B51069CA26" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V035</accession>
    <name>A0A6I8V035_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Inhibitor of growth protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Ing3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ING family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134528.2">
        <property type="nucleotide sequence ID" value="XM_002134492.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V035"/>
    <dbReference type="FunCoup" id="A0A6I8V035">
        <property type="interactions" value="1482"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901049"/>
    <dbReference type="InParanoid" id="A0A6I8V035"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V035">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16858">
        <property type="entry name" value="ING_ING3_Yng2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15585">
        <property type="entry name" value="PHD_ING3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000103">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042020">
        <property type="entry name" value="ING3_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF103">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="4 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="16">
        <location>
            <begin position="682"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="366"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="490"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="372"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="504"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="685"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="687"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="698"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="709"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="712"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="725"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="728"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="684"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="695"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="699"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002134528.2"/>
        </source>
    </evidence>
    <sequence length="740" mass="76782" checksum="94DC9CE720C98310" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V063</accession>
    <name>A0A6I8V063_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Casein kinase II subunit beta'-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in Wnt signaling. Plays a complex role in regulating the basal catalytic activity of the alpha subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134009.2">
        <property type="nucleotide sequence ID" value="XM_002133973.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V063"/>
    <dbReference type="KEGG" id="dpo:6901308"/>
    <dbReference type="InParanoid" id="A0A6I8V063"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000005">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000002">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002134009.2"/>
        </source>
    </evidence>
    <sequence length="208" mass="24162" checksum="17073363B1052BB8" modified="2020-08-12" version="1">MTDSDETGWIQWFCKQRGNEFFTFVDEEYIRDKFNLMFLDTELRNYSSALDVILDLNSGSRSDNGTHSEVDESAEKLYGMIHARFILTDRGIDLMIRKFHKGVFGTCPRVFCHRQHVLPIGLSDTPGEEMVRLYCPKCNDIYTPKSGRHRNLDGAFFGTNFPQMLFMVKPEARPKRSKTKFVPRLYGFKIHPLAYRTGPQKSLDETDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0I0</accession>
    <name>A0A6I8V0I0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135030.2">
        <property type="nucleotide sequence ID" value="XM_002134994.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0I0"/>
    <dbReference type="KEGG" id="dpo:6900662"/>
    <dbReference type="InParanoid" id="A0A6I8V0I0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0I0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135030.2"/>
        </source>
    </evidence>
    <sequence length="171" mass="19479" checksum="B3C62422549FC6DF" modified="2020-08-12" version="1">MEYGIEFFALALYTCALMLSCIAGYVRRTWPNMGRLYQNQVMPAVFGAKMTLTELKYFNATQTDGRTIVAFERKIYHVKGDSECVSVSSLRHVAVTEFCGEKLLEARHSCIGIAIDRLGKPLLVENKRNLQNEDILTDQIILDCFKERCKTKSNEYVIQNALNSAQTPTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V172</accession>
    <name>A0A6I8V172_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">eIF-4F 25 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eIF4E4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135516.2">
        <property type="nucleotide sequence ID" value="XM_002135480.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V172"/>
    <dbReference type="FunCoup" id="A0A6I8V172">
        <property type="interactions" value="1432"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900177"/>
    <dbReference type="InParanoid" id="A0A6I8V172"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V172">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000010">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002135516.2"/>
        </source>
    </evidence>
    <sequence length="223" mass="25640" checksum="F14961F2E0C1E566" modified="2020-08-12" version="1">MNTDLKLVRKTRNTTRSASSTEITAHGSPMDPVAPVIKMVSSAVKHPLEHTWTLWYLESDRSKSWEELQNEITSFDMVEDFWSLYTHIKPPSQIRLCSDYSLFKKGIQPMWEDEANKFGGRWVITTNRRSKLELDKLWLDVILILIGEAFEHTEEICGAVINMRGKQSKISVWTCNGQNESAIVHIGLKLRESLKLPPLQMKYQLHKDSMTKQGSTVKSVYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1T9</accession>
    <name>A0A6I8V1T9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6899197</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002136327.3">
        <property type="nucleotide sequence ID" value="XM_002136291.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1T9"/>
    <dbReference type="KEGG" id="dpo:6899197"/>
    <dbReference type="InParanoid" id="A0A6I8V1T9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246533">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001235">
        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000005">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="9">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein uS3 C-terminal" evidence="8">
        <location>
            <begin position="71"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="164"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002136327.3"/>
        </source>
    </evidence>
    <sequence length="209" mass="23183" checksum="BDB2866DD24B85FA" modified="2020-08-12" version="1">CPGPCVTPSRTEIIIMATKTQQVLGEKGRRIRELTAMVQKRFNFETGRIELYAEKVATRGLCAIAQAESLRYKLTGGLAVRRACYGVLRFIIESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPCNDYVETATRHVLLRQGVLGIKVKIMLPYDPKNKIGPKKPLPDNVSVVEPKEEKIYETPETEYKIPPPSKPLDDLSEAKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2T0</accession>
    <name>A0A6I8V2T0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Surfeit locus protein 6 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Surf6</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002136554.2">
        <property type="nucleotide sequence ID" value="XM_002136518.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2T0"/>
    <dbReference type="FunCoup" id="A0A6I8V2T0">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903964"/>
    <dbReference type="InParanoid" id="A0A6I8V2T0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029190">
        <property type="entry name" value="Rrp14/SURF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007019">
        <property type="entry name" value="SURF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369:SF0">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04935">
        <property type="entry name" value="SURF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal" evidence="5">
        <location>
            <begin position="103"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="54"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="271"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002136554.2"/>
        </source>
    </evidence>
    <sequence length="326" mass="37910" checksum="4D26AC1C65FE1BFB" modified="2020-08-12" version="1">MRPTSEILKEMREKYFPESVKPVSTKKKKETLLTEYTEYRDRLLDLLTAAGVPYSKEEDDDTYEDYLLSDTEEAPKKKSKKQKATGEADEDLEERLASMKNKLRQKKGPTTERQQKRREAKKMKRSKGVQKLILSSAKNLKNENVKQKKLTNGVVKHEEDGDTKDSKALIKPVKQQPVYNQDGKIVYSKVDFAATPGGKQKKSHQNPKEILKKLKDTKKQINELKEQGETEKAAEMQTDIAWKKAFDKVAGKKVKDDPKLLLKSIKKRKVEKKKAKTKWSERKQKVDYDKDKRQKKRQDNLEKRSKDKKNRKLKKASKKGRIIAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3H1</accession>
    <name>A0A6I8V3H1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137274.2">
        <property type="nucleotide sequence ID" value="XM_002137238.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3H1"/>
    <dbReference type="KEGG" id="dpo:6897090"/>
    <dbReference type="InParanoid" id="A0A6I8V3H1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026266851" evidence="2">
        <location>
            <begin position="31"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137274.2"/>
        </source>
    </evidence>
    <sequence length="109" mass="12482" checksum="797DD4AB6C7C9CEA" modified="2020-08-12" version="1">MTIPLSDMIRYRRCLSAFLVVFHAVEFVRSAPIVSPVDLSARYVTFDLAIGSKADDLPPELEMKLLQDLLQQSGMDLIRMQLLQLNLMKLLVVLDIFIWVCVYFYAVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3S7</accession>
    <name>A0A6I8V3S7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neuropeptide-like protein 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138129.1">
        <property type="nucleotide sequence ID" value="XM_002138093.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3S7"/>
    <dbReference type="FunCoup" id="A0A6I8V3S7">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="GeneID" id="6898041"/>
    <dbReference type="KEGG" id="dpo:6898041"/>
    <dbReference type="InParanoid" id="A0A6I8V3S7"/>
    <dbReference type="OMA" id="PPIYPVW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026106738" description="Neuropeptide-like protein 31" evidence="1">
        <location>
            <begin position="21"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9187" checksum="57ABC58E62BBDCA0" modified="2020-08-12" version="1">MTKTFAVLSVLALLASSTFAGPVGRKIFMSEGDLSTADSVSYGYYAAPIPAYGYGGGYKYGGYSGYGRYRSYGSYYRPPIYPVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4F6</accession>
    <name>A0A6I8V4F6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Klp54D</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138385.2">
        <property type="nucleotide sequence ID" value="XM_002138349.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4F6"/>
    <dbReference type="FunCoup" id="A0A6I8V4F6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898330"/>
    <dbReference type="InParanoid" id="A0A6I8V4F6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000080">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF33">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="141"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="712"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="721"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="233"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002138385.2"/>
        </source>
    </evidence>
    <sequence length="734" mass="80429" checksum="F8D490E680CA5ECC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4M3</accession>
    <name>A0A6I8V4M3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell division control protein 73 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6898809</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC73 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138783.2">
        <property type="nucleotide sequence ID" value="XM_002138747.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4M3"/>
    <dbReference type="KEGG" id="dpo:6898809"/>
    <dbReference type="InParanoid" id="A0A6I8V4M3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11990">
        <property type="entry name" value="RNA polymerase II accessory factor, Cdc73 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007852">
        <property type="entry name" value="Cdc73/Parafibromin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031336">
        <property type="entry name" value="CDC73_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038103">
        <property type="entry name" value="CDC73_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466">
        <property type="entry name" value="CDC73 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466:SF8">
        <property type="entry name" value="PARAFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05179">
        <property type="entry name" value="CDC73_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Cell division control protein 73 C-terminal" evidence="6">
        <location>
            <begin position="176"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="28"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002138783.2"/>
        </source>
    </evidence>
    <sequence length="347" mass="39082" checksum="6BBFAC62FBE07806" modified="2020-08-12" version="1">MAEKAEKKTLKQAISVSVDAIVKIEAKKRSAKEEAEKVAGEVGEARKRPLSQMREKPAVPLAGSCSSGEGSVRLAPHSAKDEKKPAEQQAPPKKHRKRESSKVNPRAPCRQAPKKQSQLVEVPIVPPNLETLPKYSRYAQEKFLLPVAIRGRGKKDLGINTLGSNLAKLRVWVSTRRPIIVVPQAGTSLITLHNAKEMLQHMHFVQPDMQGPLDAPHPEEVVIERDFPNGKTREYRIIDNVSRLCTDEWQRVIAVFALGPHRQFRGWPWKGDPAVIFRKVCVFHLHYQDVGVHKDLLEMAVHPLGIPKNEPHTHGGILKEFWGTLDEYVRTQSQFSFLLEEGAYFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4X9</accession>
    <name>A0A6I8V4X9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Midasin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6898950</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the midasin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138911.3">
        <property type="nucleotide sequence ID" value="XM_002138875.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V4X9">
        <property type="interactions" value="1582"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898950"/>
    <dbReference type="InParanoid" id="A0A6I8V4X9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V4X9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01460">
        <property type="entry name" value="vWA_midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000142">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000582">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001384">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000028">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004102">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040848">
        <property type="entry name" value="AAA_lid_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012099">
        <property type="entry name" value="Midasin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041190">
        <property type="entry name" value="Midasin_AAA_lid_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17865">
        <property type="entry name" value="AAA_lid_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17867">
        <property type="entry name" value="AAA_lid_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010340">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="5352"/>
            <end position="5546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="4632"/>
            <end position="5211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="2967"/>
            <end position="3022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="4289"/>
            <end position="4326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="4498"/>
            <end position="4526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="366"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4658"/>
            <end position="4695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4698"/>
            <end position="4724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4725"/>
            <end position="4740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4742"/>
            <end position="4760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4761"/>
            <end position="4796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4805"/>
            <end position="4822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4837"/>
            <end position="4847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4856"/>
            <end position="4869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4882"/>
            <end position="4894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4924"/>
            <end position="4938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4939"/>
            <end position="4948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="4949"/>
            <end position="4964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4965"/>
            <end position="4974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="4981"/>
            <end position="4998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="5009"/>
            <end position="5028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="5033"/>
            <end position="5051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="5053"/>
            <end position="5084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="5118"/>
            <end position="5132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="5148"/>
            <end position="5180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="5185"/>
            <end position="5208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR010340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002138911.3"/>
        </source>
    </evidence>
    <sequence length="5556" mass="631264" checksum="EE773087773F1870" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V594</accession>
    <name>A0A6I8V594_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6896701</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A6I8V594"/>
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    </dbReference>
    <dbReference type="KEGG" id="dpo:6896701"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="436"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
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            <end position="118"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_003736216.2"/>
        </source>
    </evidence>
    <sequence length="567" mass="65275" checksum="678CE4D6EDA6DF7D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5B0</accession>
    <name>A0A6I8V5B0_DROPS</name>
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        <submittedName>
            <fullName evidence="6">LOW QUALITY PROTEIN: headcase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">heca</name>
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    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736237.2">
        <property type="nucleotide sequence ID" value="XM_003736189.4"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026066">
        <property type="entry name" value="Headcase"/>
    </dbReference>
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        <property type="entry name" value="Headcase_mid"/>
    </dbReference>
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        <property type="entry name" value="HECA_N"/>
    </dbReference>
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        <property type="entry name" value="HEADCASE PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13425:SF3">
        <property type="entry name" value="HEADCASE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16002">
        <property type="entry name" value="Headcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15353">
        <property type="entry name" value="HECA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="73"/>
            <end position="176"/>
        </location>
    </feature>
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        <location>
            <begin position="443"/>
            <end position="642"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="189"/>
            <end position="296"/>
        </location>
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            <begin position="323"/>
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            <begin position="718"/>
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            <begin position="1"/>
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            <begin position="13"/>
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            <begin position="224"/>
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            <begin position="282"/>
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            <dbReference type="Pfam" id="PF15353"/>
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        <source>
            <dbReference type="Pfam" id="PF16002"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_003736237.2"/>
        </source>
    </evidence>
    <sequence length="1130" mass="120587" checksum="0B52E7BE0347F672" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5Q9</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein isoform X1</fullName>
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        <name evidence="5" type="primary">LOC6898602</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_002138575.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5Q9"/>
    <dbReference type="GeneID" id="6898602"/>
    <dbReference type="KEGG" id="dpo:6898602"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
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        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002138611.1"/>
        </source>
    </evidence>
    <sequence length="125" mass="13572" checksum="FEB1A37299E53EF0" modified="2020-08-12" version="1">MSRRLCALFVMLLLHLAASAPPTLVPQIRIPGFQLEPLDNTYYLSIQHPDGTIRQESVKEIAPGQLQVQGVINQPFEEHGTSLVVTYQAGANGYVAKYTYGKGPPTPPTQLPQFLSPNALKSTAG</sequence>
</entry>
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        <submittedName>
            <fullName evidence="3">Peptide tarsal-less AA</fullName>
        </submittedName>
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        <name evidence="3" type="primary">tal-AA</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="RefSeq"/>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_003736624.1"/>
        </source>
    </evidence>
    <sequence length="32" mass="3869" checksum="F6A233EA4BAE0AC8" modified="2020-08-12" version="1">MLDPTGTYRRPRDTKDTRQKRRQDCLDPTGQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7A3</accession>
    <name>A0A6I8V7A3_DROPS</name>
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        <recommendedName>
            <fullName evidence="9">Sidoreflexin</fullName>
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        <name evidence="11" type="primary">Sfxn1-3</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <text evidence="2 9">Belongs to the sideroflexin family.</text>
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    <dbReference type="InParanoid" id="A0A6I8V7A3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:mitochondrial inner membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0140300">
        <property type="term" value="P:serine import into mitochondrion"/>
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        <property type="entry name" value="Mtc"/>
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        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SIDEROFLEXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_003736809.3"/>
        </source>
    </evidence>
    <sequence length="346" mass="38334" checksum="72969B865D3B28CA" modified="2020-08-12" version="1">MADPQNPIPNPTPVLERDQFAAFSKMTPLPRVDIDQPKYDQSTYVGRAKHFLLVTNPLNVLATNTKLEQARQIVLKYRAGKEVTECRTIEDVWKAKYLYDSAFHPETGDKQIIIGRMSAQMPMNMLITGGMMAFYKSTGAVVFWQWMNQTFNAIVNYTNRSGTSPLSNSQLLTSYCLATGGALATALSLNRAVKNMNPLVGRMVPLVAVAAANCINIPCMRMQEIRNGVVLLDEKNAEVGVSKKAACVGISAVIVSRICMALPGMTLTPMLMNLLERRGFLAKYPKSNAPIQTLFCGFVLIFATPLGCAFFKQRAAIKVASLEPEVRDNIEKKRPELDTVWYNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7I8</accession>
    <name>A0A6I8V7I8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Protein charlatan isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">chn</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_004444210.3">
        <property type="nucleotide sequence ID" value="XM_004444153.3"/>
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        <property type="nucleotide sequence ID" value="XM_015184481.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001924">
        <property type="entry name" value="Charlatan, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF34">
        <property type="entry name" value="PR_SET DOMAIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="360"/>
            <end position="388"/>
        </location>
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            <begin position="230"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="394"/>
            <end position="422"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="518"/>
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            <end position="640"/>
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            <end position="880"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <end position="1011"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1166"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1228"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="289"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="398"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="500"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="610"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="868"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="974"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="991"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1228"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1239"/>
            <end position="1248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_004444210.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015039967.2"/>
        </source>
    </evidence>
    <sequence length="1335" mass="142625" checksum="1F51473F1108A202" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V862</accession>
    <name>A0A6I8V862_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">SUMO-conjugating enzyme UBC9-B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6902813</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035819.2">
        <property type="nucleotide sequence ID" value="XM_015180333.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V862"/>
    <dbReference type="KEGG" id="dpo:6902813"/>
    <dbReference type="InParanoid" id="A0A6I8V862"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUMO-conjugating enzyme UBC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015035819.2"/>
        </source>
    </evidence>
    <sequence length="159" mass="18121" checksum="224A6CA0735F2F87" modified="2020-08-12" version="1">MSATTIARLSEERKAWRKDHPYGFVARPAKNPDGTLNLMIWECAIPGKKSTPWEGGLYKLRMIFKDDYPTSPPKCKFEPPLFHPNVYPSGTVCLSLLDEKKAWRPSITIKQVLVGIQELLDNPNAKDPAQAEAYTAYTQNRQQYEMRLRAQAHMMTPIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V867</accession>
    <name>A0A6I8V867_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Slob isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Slob</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015035828.2">
        <property type="nucleotide sequence ID" value="XM_015180342.2"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V867"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000908">
        <property type="entry name" value="Slowpoke binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="858"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="924"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1066"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1113"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1336"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="858"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="924"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1075"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1114"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1141"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1337"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1408"/>
            <end position="1417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015035828.2"/>
        </source>
    </evidence>
    <sequence length="1417" mass="155045" checksum="8B943703C61F42E3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V878</accession>
    <name>A0A6I8V878_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Single domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6903890</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015035089.2">
        <property type="nucleotide sequence ID" value="XM_015179603.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V878"/>
    <dbReference type="FunCoup" id="A0A6I8V878">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903890"/>
    <dbReference type="InParanoid" id="A0A6I8V878"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V878">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029277">
        <property type="entry name" value="SVWC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15430">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01318">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Single" evidence="4">
        <location>
            <begin position="68"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015035089.2"/>
        </source>
    </evidence>
    <sequence length="163" mass="17016" checksum="5E5F0FA9390C6168" modified="2020-08-12" version="1">MGPPAKGPPIKRLPLVLSGVPQFLFRSVYDQFPKMKYIFFLVLVGLMPLAKSLVHPANLGPSGDGKGCKGPKGDILPGEMVQDEKVCGVFYCATDKGDGFIHYCQLTATFAECSGTTVSTTVDFPDCCWLCVKDKKCDKEGGAEAGGAGGAGGAGVATEKPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V982</accession>
    <name>A0A6I8V982_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6903222</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036410.2">
        <property type="nucleotide sequence ID" value="XM_015180924.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:6903222"/>
    <dbReference type="InParanoid" id="A0A6I8V982"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="243"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="285"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015036410.2"/>
        </source>
    </evidence>
    <sequence length="397" mass="43531" checksum="C25821B2A4704388" modified="2020-08-12" version="1">MSDIHPRPEAKEKAASGISASHRSEPTYAPQRVEVSGGSRPDETPTEAQQLSTFVAASDHHVEEVVLCVLISESVSLMAVGKADNLIHVFGLKLNSNGADADWIADGGFNLVGHEVPATHGAFSDDRQHLVTWAPDLVLRQWNLNSQKCTGIYANTHSHVSRILFEAHGLFFTTIDELGYAFIWMIADSGSSNELRYVDKNYYSHPLTACAFHPKITYLVTGSTDGMVRMWNMSIGTTAVRTFLGHKTPITALAFSICGYYLVAGATDGLLIVWNLKSQVLLRRLTHHSGAITSIGFSPNNGRLAVGSMDTQMSVWDFELLVRTTVAGRPSATEDMLVGTRASECGPIFEVKFVSDSHLMAICVDKREASAESEEDLFQCFVEMEAEESELEPEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAP7</accession>
    <name>A0A6I8VAP7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mannosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">Alg12</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15 16">MV-25-SWS-2005</strain>
            <strain evidence="14">MV2-25</strain>
            <tissue evidence="15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man(7)GlcNAc(2)-PP-dolichol to produce Man(8)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29535"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19518"/>
            <dbReference type="Rhea" id="RHEA-COMP:19519"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132517"/>
            <dbReference type="ChEBI" id="CHEBI:132519"/>
            <dbReference type="EC" id="2.4.1.260"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:29536"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_001358592.3">
        <property type="nucleotide sequence ID" value="XM_001358555.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037285.2">
        <property type="nucleotide sequence ID" value="XM_015181799.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAP7"/>
    <dbReference type="KEGG" id="dpo:4801513"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VAP7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052917">
        <property type="term" value="F:dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF1">
        <property type="entry name" value="DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="161"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="607"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358592.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015037285.2"/>
        </source>
    </evidence>
    <sequence length="685" mass="77284" checksum="89CBDC3844002568" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGC9</accession>
    <name>A0A6I8VGC9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">ATP-citrate synthase</fullName>
            <ecNumber evidence="19">2.3.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">ATP-citrate (pro-S-)-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Citrate cleavage enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24 25 26" type="primary">ATPCL</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">MV-25-SWS-2005</strain>
            <strain evidence="23">MV2-25</strain>
            <tissue evidence="24">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="25 26" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25 26">MV-25-SWS-2005</strain>
            <strain evidence="23">MV2-25</strain>
            <tissue evidence="25 26">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate in multiple biochemical reactions in protein, carbohydrate and lipid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>oxaloacetate + acetyl-CoA + ADP + phosphate = citrate + ATP + CoA</text>
            <dbReference type="Rhea" id="RHEA:21160"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.8" evidence="19"/>
    <dbReference type="RefSeq" id="XP_015040766.1">
        <property type="nucleotide sequence ID" value="XM_015185280.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040768.1">
        <property type="nucleotide sequence ID" value="XM_015185282.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040769.1">
        <property type="nucleotide sequence ID" value="XM_015185283.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGC9"/>
    <dbReference type="GeneID" id="4805659"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080975">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VGC9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003878">
        <property type="term" value="F:ATP citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06100">
        <property type="entry name" value="CCL_ACL-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000004">
        <property type="entry name" value="ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000009">
        <property type="entry name" value="ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000003">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000004">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.110:FF:000003">
        <property type="entry name" value="ATP-citrate synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000024">
        <property type="entry name" value="Probable ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014608">
        <property type="entry name" value="ATP-citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032263">
        <property type="entry name" value="Citrate-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056749">
        <property type="entry name" value="Citrate_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118:SF42">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16114">
        <property type="entry name" value="Citrate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24948">
        <property type="entry name" value="Citrate_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036511">
        <property type="entry name" value="ATP_citrt_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 19" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="17 19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CoA-binding" evidence="22">
        <location>
            <begin position="506"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="20">
        <location>
            <position position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR036511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR036511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_015040766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_015040768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_015040769.1"/>
        </source>
    </evidence>
    <sequence length="1114" mass="121765" checksum="B1B90E0AD924325E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHL4</accession>
    <name>A0A6I8VHL4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC26532514</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015041305.2">
        <property type="nucleotide sequence ID" value="XM_015185819.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VHL4"/>
    <dbReference type="FunCoup" id="A0A6I8VHL4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532514"/>
    <dbReference type="InParanoid" id="A0A6I8VHL4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VHL4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009125">
        <property type="entry name" value="ATPMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34038">
        <property type="entry name" value="ATP SYNTHASE MEMBRANE SUBUNIT DAPIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34038:SF1">
        <property type="entry name" value="ATP SYNTHASE MEMBRANE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14960">
        <property type="entry name" value="ATP_synth_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="207"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015041305.2"/>
        </source>
    </evidence>
    <sequence length="299" mass="32645" checksum="7E1F45408140C5FF" modified="2020-08-12" version="1">MSEGFKKHFNASTMNGRANVAKVTYATMALLYIVYRMRRGSGKKPKDGSGMLADGSCSCDQKSPPEGHDSDTGFYETDPSCGSCRERSERAMSEYDREQQRRSAAKARQDHSDECRAEPPSPPSSSSPPGSGGVATPRRKCPCENPHRKSIYDMKSSRNDTRSFMQRQHQHDAQDADDVRPASEIVGQVGGAVYRVLSGVFNAVMGGESSVTSTTSPPKSQRNQEERRADQADHTQNEDGEVTAVGYEPMDCECGLNTPLKPRTGPRCCESPTGANEGQAQPEDAQYNDSYNDGYASVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VI08</accession>
    <name>A0A6I8VI08_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein FAM60A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4815219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041773.2">
        <property type="nucleotide sequence ID" value="XM_015186287.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041774.2">
        <property type="nucleotide sequence ID" value="XM_015186288.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VI08"/>
    <dbReference type="KEGG" id="dpo:4815219"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VI08">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026065">
        <property type="entry name" value="FAM60A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13422">
        <property type="entry name" value="SIN3-HDAC COMPLEX-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13422:SF12">
        <property type="entry name" value="SIN3-HDAC COMPLEX-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15396">
        <property type="entry name" value="FAM60A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="410"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015041773.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015041774.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="65802" checksum="E0C8662196D0676C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKT2</accession>
    <name>A0A6I8VKT2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">LOC4813385</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042993.2">
        <property type="nucleotide sequence ID" value="XM_015187507.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042994.2">
        <property type="nucleotide sequence ID" value="XM_015187508.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VKT2"/>
    <dbReference type="KEGG" id="dpo:4813385"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12510">
        <property type="entry name" value="RRM1_RBM12_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12515">
        <property type="entry name" value="RRM5_RBM12_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12254">
        <property type="entry name" value="RRM_hnRNPH_ESRPs_RBM12_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="420"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="534"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="803"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="152"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="500"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="650"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="890"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="395"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="650"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="674"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="749"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="902"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="931"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="973"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015042993.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015042994.2"/>
        </source>
    </evidence>
    <sequence length="1082" mass="119704" checksum="94F25BD11153A402" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKV4</accession>
    <name>A0A6I8VKV4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA primase</fullName>
            <ecNumber evidence="12">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">Prim1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15">MV-25-SWS-2005</strain>
            <tissue evidence="14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="12"/>
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        <property type="nucleotide sequence ID" value="XM_015187987.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239659.1">
        <property type="nucleotide sequence ID" value="XM_033383768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VKV4"/>
    <dbReference type="KEGG" id="dpo:4813935"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
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    </dbReference>
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        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015043473.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033239659.1"/>
        </source>
    </evidence>
    <sequence length="438" mass="50849" checksum="AE4FFCCA4157425A" modified="2020-08-12" version="1">MSKQATDLETPVPQQTVVPAFDPTILPDMLPVYYRRLFPHLPFYRWLSYGLSEDGVFCNREISFTLHDDIYIRYLCFETQAELEKEICSRNPYKIDIGPVMHTRPTNFRIVPGGLTPVQRELVFDIDMTDYDEVRTCCSGAEVCLKCWKFMVLAARVLDLALREDFGFEHILWVFSGRRGIHCWVCDHQARHLDGRGRYAVAEYFNIVSYVNFMGSNSPRCQLGDRSHHSFKRALKIIEPLFEEIIIEDQNLFGTPKGVNKLLHMIHDDGARTEFEGYLQKSHEDGAHSRLIWESFIKYSNSMRTSTANVWSRKLKNIVQEVQLCLLYPRLDINVTRGFNHLLKSPFCIHPATGKVCVPFSVNAVAKFDPTTVPTITQLLHEINAYDDKTKSYMEAPEDKSRIKDHKKTSMFKGVVVFEEFLRKLERSNKERTNVLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLZ8</accession>
    <name>A0A6I8VLZ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900293</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015044081.2">
        <property type="nucleotide sequence ID" value="XM_015188595.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLZ8"/>
    <dbReference type="InParanoid" id="A0A6I8VLZ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VLZ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015044081.2"/>
        </source>
    </evidence>
    <sequence length="260" mass="30905" checksum="040EEA195482A928" modified="2020-08-12" version="1">MPANKTTKPLKWHRKVTTIKKWNDEEIKSMLTYISRHGNIEKPTARKYYTKLIEETKISGSWNTFKCKLRYLKGTLQKANTWMKTKGAEIDDPVRVRETLLQLCPHYDQLVDMFGADFNFESSMSNSAWNTDFNHPRSSIFSNDDSLNEEYLRSETESSVDPIDCSDSLYETELQNHQSRIEEKTMDHSCPAAKKIDELDFKEAILQLEVEKFHWLKEKELRRMKFEEKKLETAVELRKLELEQEERIALYEIKMKYKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMS3</accession>
    <name>A0A6I8VMS3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4800433</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033232361.1">
        <property type="nucleotide sequence ID" value="XM_033376470.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800433"/>
    <dbReference type="InParanoid" id="A0A6I8VMS3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022412">
        <property type="term" value="P:cellular process involved in reproduction in multicellular organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09823">
        <property type="entry name" value="peroxinectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.640.10:FF:000003">
        <property type="entry name" value="chorion peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF109">
        <property type="entry name" value="CHORION PEROXIDASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
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</entry>
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        <source>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_033232963.1"/>
        </source>
    </evidence>
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</entry>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="RefSeq"/>
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            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR041541">
        <property type="entry name" value="Glutaminase_EF-hand"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03814">
        <property type="entry name" value="Gln_ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF29">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17959">
        <property type="entry name" value="EF-hand_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Glutaminase EF-hand" evidence="11">
        <location>
            <begin position="142"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="599"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="667"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="696"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033233241.1"/>
        </source>
    </evidence>
    <sequence length="755" mass="84038" checksum="5F35695214697655" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VS84</accession>
    <name>A0A6I8VS84_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Keratin, type I cytoskeletal 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233763.1">
        <property type="nucleotide sequence ID" value="XM_033377872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VS84"/>
    <dbReference type="KEGG" id="dpo:117183576"/>
    <dbReference type="InParanoid" id="A0A6I8VS84"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026061308" evidence="1">
        <location>
            <begin position="19"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233763.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="13771" checksum="D0C1E931CCC2B8AD" modified="2020-08-12" version="1">MGPVVAVVMLLALASTLANPTYRGSYGDYQSGNSLISGTKTGGPFATNNLDAALISGGTSGGPFQNAQKSGGLIIGSHTGGPFGSKSQNSDLISGTRTGGGPIGSDGHTEGPFDGSSVGGGLYNGLNTGGPFRDHYYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VU15</accession>
    <name>A0A6I8VU15_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234565.1">
        <property type="nucleotide sequence ID" value="XM_033378674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VU15"/>
    <dbReference type="KEGG" id="dpo:117183728"/>
    <dbReference type="InParanoid" id="A0A6I8VU15"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234565.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="38687" checksum="3E9CA5A0C7A1C76A" modified="2020-08-12" version="1">MAPRIRASRTTESRRDRGINSYRCRVCRGIHPLRTCHHFLRLSPEKRLRAVLINKYCGNCLAHQHSGQDCRSGGHAGCVAKTTTLYSTYPRLVVQSARPQEHRRHLQRPTSNADLGVQPAQPRLHHVSRPVAGPPAPSVDSLLQHRSLIILPTALVVLDTGSKNFETAALIDPCTPVSCIDDSLASAFKLPTTCVGDEKVCTAVIRAKMGDFQLETLLKVEPRVRIRTPIRQLSDSVRARFGDLRLADEQFHRPATISLILGADVYPKVIQPGFLAREDGLPVAQSTVFGRLGHPIILCNPARGGEDVQASNRQFPRTVPAPRASVPARARALGGALGSTIHDPHGAHRSIKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVY5</accession>
    <name>A0A6I8VVY5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hppy</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_033235113.1">
        <property type="nucleotide sequence ID" value="XM_033379222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VVY5"/>
    <dbReference type="FunCoup" id="A0A6I8VVY5">
        <property type="interactions" value="1413"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VVY5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080094">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VVY5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06613">
        <property type="entry name" value="STKc_MAP4K3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000031">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF18">
        <property type="entry name" value="HAPPYHOUR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="26"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="13">
        <location>
            <begin position="910"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="421"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="639"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="784"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="834"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="421"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="502"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="639"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="785"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="836"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="873"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033235113.1"/>
        </source>
    </evidence>
    <sequence length="1269" mass="136295" checksum="4AE942D1C23380AE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXC8</accession>
    <name>A0A6I8VXC8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MICOS complex subunit MIC13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117184004</name>
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    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
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    </reference>
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        <property type="entry name" value="TMEM214"/>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033235745.1"/>
        </source>
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    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20254" checksum="A088CCB9B05FDE0D" modified="2020-08-12" version="1">MHHLTPKCKWCGIFVVLMISVALYYDTQINGQGIFAESATGKALKDAGVLPLLQKGCDVALVAGARGFQCYEKYVDPYVVIVSQMVEDLVRQSLSDAGNVYDWAKGYFNQSWTLQVDVVDQYAPNLSKKLKAFASGAMESYNETATILDNNFHQMIIFAWKCNREFHEKVDEYAKRQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W419</accession>
    <name>A0A6I8W419_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">BTB/POZ domain-containing protein 7</fullName>
        </submittedName>
    </protein>
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        <name evidence="4 5" type="primary">LOC4811768</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="153"/>
            <end position="222"/>
        </location>
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    <feature type="domain" description="BTB" evidence="2">
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            <begin position="257"/>
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        </location>
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        <location>
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            <end position="800"/>
        </location>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1013"/>
        </location>
    </feature>
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        <location>
            <begin position="791"/>
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        </location>
    </feature>
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        <location>
            <begin position="919"/>
            <end position="936"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033238062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033238063.1"/>
        </source>
    </evidence>
    <sequence length="1013" mass="111586" checksum="1A509978394D7F28" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7V1</accession>
    <name>A0A6I8W7V1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger protein 443-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC117184743</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033383564.1"/>
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    <dbReference type="KEGG" id="dpo:117184743"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
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    </dbReference>
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    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
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        <location>
            <begin position="230"/>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="258"/>
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        <location>
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        <location>
            <begin position="144"/>
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        </location>
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            <begin position="171"/>
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        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033239455.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35233" checksum="51AB7E8C41462192" modified="2020-08-12" version="1">MNNQCRACGSAVYNTKDRNLFQKQNAKLLLNFNLVTNNSLKKDPRLPSCLCASCVEYLNRADIFRARFLETQENLLQQRAGLPENDLPTDDETPGETSNSEVKAQDADSLMSGLENELGGKTGFDGTDAYETAQVADSLITSLQKDSGHEKKKSSITENIIKAPKQLADNSTFRSSPSTTPTSRSPLSTTPTSSSTRPSRLTSKNAAQSDKRHPLPKKILKRRSCTMNKLKCKFCEQTFVSPFELSCHMRSHTAETSCKCSYCDRAFSTKGNTIRHERTHTRQAAFVCPTCDMSFTLATVLKRHISLYHTQMSMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8Q2</accession>
    <name>A0A6I8W8Q2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Adult enhancer factor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Aef1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033239657.1">
        <property type="nucleotide sequence ID" value="XM_033383766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W8Q2"/>
    <dbReference type="KEGG" id="dpo:4813928"/>
    <dbReference type="InParanoid" id="A0A6I8W8Q2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W8Q2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000621">
        <property type="entry name" value="FLT3-interacting zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002104">
        <property type="entry name" value="Si:ch211-266d19.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF23">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="134"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="293"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033239657.1"/>
        </source>
    </evidence>
    <sequence length="458" mass="47985" checksum="6032DDA803506E5D" modified="2020-08-12" version="1">MMHIKSLPHAHAHAAATAMSSNCDIVIVASQPQTTIANNNNNDTVTQATHPGHVAAAAQQQQQQQQHHQQQQAQQQQAQQQQAQQQQSSGPPSAPPAPAELALPFQMHLTGISAEAHSAAQAAAMAAAQAAAAQAAAADQQQPPPPPPPHLVHLATHSPTMSNDHYLANGHGEHQGEGTGSNANSGGGGGGGGGARDQEKPFHCTVCDRRFRQLSTLTNHVKIHTGEKPYKCNVCDKTFRQSSTLTNHLKIHTGEKPYNCNYCPKHFRQLSTLANHIKIHTAVRVRHMQKAIPAVQHAQQPHKDPCHGQGLRSCQDQNGGGGGVTRTDGVGGASSSSAAAPSSAAATATASAPPTASASASYSSPCTCPPHRPSARNNYYHSALHHVCGATPATTTAAATSSWKWQLSPSFQCGLVGRLVQRHAAGSCDSGWELCHHEWGGGGPHSTHARRAAAAWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W939</accession>
    <name>A0A6I8W939_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Uncharacterized protein UBL3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6 7 8 9 10 11 12" type="primary">UBL3</name>
    </gene>
    <organism evidence="3 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
            <tissue evidence="4 5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239896.1">
        <property type="nucleotide sequence ID" value="XM_033384005.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239897.1">
        <property type="nucleotide sequence ID" value="XM_033384006.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239898.1">
        <property type="nucleotide sequence ID" value="XM_033384007.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239899.1">
        <property type="nucleotide sequence ID" value="XM_033384008.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239900.1">
        <property type="nucleotide sequence ID" value="XM_033384009.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239901.1">
        <property type="nucleotide sequence ID" value="XM_033384010.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239902.1">
        <property type="nucleotide sequence ID" value="XM_033384011.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239903.1">
        <property type="nucleotide sequence ID" value="XM_033384012.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239904.1">
        <property type="nucleotide sequence ID" value="XM_033384013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W939"/>
    <dbReference type="KEGG" id="dpo:26532244"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W939">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd17048">
        <property type="entry name" value="Ubl_UBL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040015">
        <property type="entry name" value="UBL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039540">
        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047977">
        <property type="entry name" value="UBL3_Ubl_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169:SF0">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13881">
        <property type="entry name" value="Rad60-SLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="177"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033239898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033239899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033239900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033239901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033239902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033239903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033239904.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="30699" checksum="D56F8B96A853E64A" modified="2020-08-12" version="1">MSATSSVETPTTQNLQSKTAIVISEQNVQSQYVKRSFSVSLSVSSSTSLGAAGSAISLSVDERTAVIAPPQLLLQSSTSSTSTNVAATIAAVHQRKSYSLEQKESSLRHQVKPNQQLANISLPVAMTSSEVTPSLTGASVAILGTLEATSRHCTNATGKIAPLTSQKMHRTIPSDKINLRLILVSGKTKEFIFNPSDSAGDIAQTVFDNWPTDWTREAVSKAEILRLIYQGRFLHCNVTLGALGLPLGKTTVMHLVPRDNLPEPNSQDQRQKSKGGSGSCCSTTCCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WB15</accession>
    <name>A0A6I8WB15_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4812155</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352901.2">
        <property type="nucleotide sequence ID" value="XM_001352865.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240467.1">
        <property type="nucleotide sequence ID" value="XM_033384576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WB15"/>
    <dbReference type="KEGG" id="dpo:4812155"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WB15">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="275"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="392"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="576"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="655"/>
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        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="548"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="642"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352901.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033240467.1"/>
        </source>
    </evidence>
    <sequence length="733" mass="81112" checksum="261D96BD51955A74" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBA2</accession>
    <name>A0A6I8WBA2_DROPS</name>
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        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">LOC117185072</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240678.1">
        <property type="nucleotide sequence ID" value="XM_033384787.1"/>
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    <dbReference type="KEGG" id="dpo:117185072"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="RNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041:SF78">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23041">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="156"/>
            <end position="196"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="76"/>
            <end position="149"/>
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        <location>
            <begin position="9"/>
            <end position="22"/>
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        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033240678.1"/>
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    </evidence>
    <sequence length="209" mass="22609" checksum="C0F2755D315DC31C" modified="2020-08-12" version="1">MSSENLQVSSSTGTSTQSSQSTPNASVSSNISDGENSGSRAAPPMVTAGGILNRIVSGIRSAYLEILVRIPMVVPRNNRSDSTSNRAPEAAIGLRTPNSLSTDRIRAPSTSRRRRHASTNTATTGPTASDAEVSGPSRPKRQRTDSNESLEETYNCPICIESVSSRDPVATKCGHVFCRQCIRTVIRRFHKCPVCRMRSTLRQLKSITM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBF2</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">LOC6899941</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM135 family.</text>
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        <property type="nucleotide sequence ID" value="XM_033384845.1"/>
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        <property type="interactions" value="157"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="entry name" value="Protein FAM135A isoform a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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        <property type="entry name" value="DUF3657"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF5">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12394">
        <property type="entry name" value="DUF3657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="3">
        <location>
            <begin position="1252"/>
            <end position="1444"/>
        </location>
    </feature>
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        <location>
            <begin position="48"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="464"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="759"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="878"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1144"/>
            <end position="1202"/>
        </location>
    </feature>
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        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="691"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="759"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="811"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="836"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="902"/>
            <end position="911"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="933"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1169"/>
            <end position="1179"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007949"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033240736.1"/>
        </source>
    </evidence>
    <sequence length="1522" mass="168135" checksum="E6BEE6E5B1D7C670" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDM3</accession>
    <name>A0A6I8WDM3_DROPS</name>
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        <submittedName>
            <fullName evidence="10">ATP-binding cassette sub-family A member 3-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6900491</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033240664.1">
        <property type="nucleotide sequence ID" value="XM_033384773.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900491"/>
    <dbReference type="InParanoid" id="A0A6I8WDM3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF250">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="834"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1021"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1060"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1097"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1124"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1151"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1204"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="482"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="1253"/>
            <end position="1489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033240664.1"/>
        </source>
    </evidence>
    <sequence length="1586" mass="180521" checksum="7F5320DC7A13083F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>B5DMU8</accession>
    <accession>A0A6I8V0C4</accession>
    <name>B5DMU8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA-dihydrouridine(20) synthase [NAD(P)+]-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Dus2</name>
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    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_002134128.2">
        <property type="nucleotide sequence ID" value="XM_002134092.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DMU8"/>
    <dbReference type="SMR" id="B5DMU8"/>
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        <property type="interactions" value="1265"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DMU8"/>
    <dbReference type="GeneID" id="6901442"/>
    <dbReference type="KEGG" id="dpo:6901442"/>
    <dbReference type="eggNOG" id="KOG2334">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_3_0_1"/>
    <dbReference type="InParanoid" id="B5DMU8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247947">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DMU8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19871">
        <property type="entry name" value="DSRM_DUS2L"/>
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        <property type="entry name" value="DUS_like_FMN"/>
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        <property type="entry name" value="Aldolase class I"/>
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        <property type="entry name" value="Aldolase_TIM"/>
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        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
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    <dbReference type="InterPro" id="IPR044463">
        <property type="entry name" value="DUS2_DSRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052582">
        <property type="entry name" value="tRNA-DUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002134128.2"/>
        </source>
    </evidence>
    <sequence length="481" mass="54078" checksum="AB20EB5236950FD7" modified="2016-01-20" version="2">MQFLSRSRWFSFLKKFQIMTIPKPRQKLDYRNKLMLAPMVRVGTLPMRLLALEMGADIVYTEELVDLKLIKSIRRHNPALGTVDFVDPSDGTVVFRTCALETSRLVLQMGTSDATRALAVGKLLQQDISGLDINMGCPKEFSIKGGMGAALLADPDKAALILRTLCSNLDIPVTCKIRILPDLDDTISLVQKLADTGIAAIGIHARTRDERPQHPPHPDVLRAVAKAVSIPIIANGGSRNVQKYEDILKFQLECGADSVMVARAAQINVSIFRRDGLLPMDELIERYLRLCVDYDNAPHNAKYCVQNMLKELQETPRGKRFLQCQTLQQICEIWHLGDYCRRKQKELRTLGNSGRAEVEPPEAQAKRQKLEEAATAITDEYEGVICRSMPFLRSTYASDNHLPKTVLYVYAGKMGKSPPAYETQQCDKLFRAICSYDDQRYSSTFWEKNKKQAEQGAALVALLHIGQLQEEPLRENGSLIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>P54364</accession>
    <accession>Q29C76</accession>
    <name>JANA_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Sex-regulated protein janus-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">janA</name>
        <name type="ORF">GA20699</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1995" name="Mol. Gen. Genet." volume="246" first="549" last="560">
            <title>Separate cis-regulatory sequences control expression of serendipity beta and janus A, two immediately adjacent Drosophila genes.</title>
            <authorList>
                <person name="Yanicostas C."/>
                <person name="Ferrer P."/>
                <person name="Vincent A."/>
                <person name="Lepesant J.-A."/>
            </authorList>
            <dbReference type="PubMed" id="7700229"/>
            <dbReference type="DOI" id="10.1007/BF00298961"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">JanA and janB regulate somatic sex differentiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the janus family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="AAB33912" version="2"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="EAL26770" version="2"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="S77099">
        <property type="protein sequence ID" value="AAB33912.2"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL26770.2"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54364"/>
    <dbReference type="SMR" id="P54364"/>
    <dbReference type="FunCoup" id="P54364">
        <property type="interactions" value="1357"/>
    </dbReference>
    <dbReference type="STRING" id="46245.P54364"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0303050">
        <property type="protein sequence ID" value="FBpp0292169"/>
        <property type="gene ID" value="FBgn0015151"/>
    </dbReference>
    <dbReference type="GeneID" id="4800256"/>
    <dbReference type="KEGG" id="dpo:4800256"/>
    <dbReference type="CTD" id="43569"/>
    <dbReference type="eggNOG" id="ENOG502S4DR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="P54364"/>
    <dbReference type="OMA" id="VRGYSWA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0015151">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101006">
        <property type="term" value="F:protein histidine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.20.20:FF:000001">
        <property type="entry name" value="14 kDa phosphohistidine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.20">
        <property type="entry name" value="Janus/Ocnus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007702">
        <property type="entry name" value="Janus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038596">
        <property type="entry name" value="Janus_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12258:SF5">
        <property type="entry name" value="BCDNA.GH02250-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12258">
        <property type="entry name" value="JANUS-A/JANUS-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05005">
        <property type="entry name" value="Ocnus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143724">
        <property type="entry name" value="PHP14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0221">Differentiation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0726">Sexual differentiation</keyword>
    <feature type="chain" id="PRO_0000206160" description="Sex-regulated protein janus-A">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="149" mass="16744" checksum="4D74D5A2FB0DA978" modified="2006-05-30" version="2">MLKAAAAALYRKNSNFLQGLRLLHKMSDQDLAKIPLVDIDEEGIFKYILIRVTGKETADGTEPSKLVVRGYADCEWHADIYERTQGTIKGTGLDTECLGGGRIEHNPEKKYLKVYGHSTGYGKADHAESKRVLLTKYKNYEIETSDEGY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q290L5</accession>
    <name>FBSP1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box/SPRY domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Fsn</name>
        <name type="ORF">GA18323</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required in the presynaptic motoneuron to down-regulate the levels of wnd and restrain synaptic terminal growth at the neuromuscular junction (NMJ).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of an E3 ubiquitin ligase complex composed of hiw and Fsn.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FBXO45/Fsn family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL25347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001360772.1">
        <property type="nucleotide sequence ID" value="XM_001360735.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q290L5"/>
    <dbReference type="SMR" id="Q290L5"/>
    <dbReference type="FunCoup" id="Q290L5">
        <property type="interactions" value="1239"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q290L5"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0278223">
        <property type="protein sequence ID" value="FBpp0276661"/>
        <property type="gene ID" value="FBgn0078328"/>
    </dbReference>
    <dbReference type="GeneID" id="4804168"/>
    <dbReference type="KEGG" id="dpo:4804168"/>
    <dbReference type="CTD" id="36460"/>
    <dbReference type="eggNOG" id="KOG3953">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046756_1_0_1"/>
    <dbReference type="InParanoid" id="Q290L5"/>
    <dbReference type="OMA" id="ATKRASM"/>
    <dbReference type="PhylomeDB" id="Q290L5"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078328">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045886">
        <property type="term" value="P:negative regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060386">
        <property type="term" value="P:synapse assembly involved in innervation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12907">
        <property type="entry name" value="SPRY_Fbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000069">
        <property type="entry name" value="F-box/SPRY domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000017">
        <property type="entry name" value="F-box/SPRY domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035784">
        <property type="entry name" value="SPRY_FBXO45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF7">
        <property type="entry name" value="F-BOX_SPRY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0524">Neurogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0770">Synapse</keyword>
    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="chain" id="PRO_0000383315" description="F-box/SPRY domain-containing protein 1">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="3"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="61"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9V6L9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EAL25347.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="28760" checksum="4BBE9DD0AAFA0F17" modified="2006-04-04" version="1">MVDRVAALCNYNVLEVVFSYLDLNDLGRCSQVCKSWFHFLNDENSDVWRFHCLNKLPKEVTKSELLSPVPTYKTKLRAFFHSWNPSDCSRNVYIKPNGFTLHRNPVAQSTDAARAKIGFRHGRHAWEVIWEGPLGTVAVIGISTKEAALQCHGYVALLGSDDQSWGWNLVENHLLHNGDMQGGYPLLNNAPKYQVGERIRVILDCDDNTLSFEKNYEFLGVAFRGLPDKKLFPTVSAVYGNTEVSMVYCGTPLDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-03" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q29B55</accession>
    <name>NPF_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Neuropeptide F</fullName>
            <shortName>NPF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">npf</name>
        <name type="ORF">GA10258</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">An integral part of the sensory system that mediates food signaling, providing the neural basis for the regulation of food response; coordinates larval foraging and social behavior changes during development. May have a hormonal role in females.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NPY family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL27144.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29B55"/>
    <dbReference type="SMR" id="Q29B55"/>
    <dbReference type="FunCoup" id="Q29B55">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29B55"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0284200">
        <property type="protein sequence ID" value="FBpp0282638"/>
        <property type="gene ID" value="FBgn0070316"/>
    </dbReference>
    <dbReference type="GeneID" id="4800796"/>
    <dbReference type="KEGG" id="dpo:4800796"/>
    <dbReference type="CTD" id="42018"/>
    <dbReference type="eggNOG" id="ENOG502T6VN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2294604_0_0_1"/>
    <dbReference type="InParanoid" id="Q29B55"/>
    <dbReference type="OMA" id="CIVEASK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070316">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030536">
        <property type="term" value="P:larval feeding behavior"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035177">
        <property type="term" value="P:larval foraging behavior"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032095">
        <property type="term" value="P:regulation of response to food"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035176">
        <property type="term" value="P:social behavior"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0027">Amidation</keyword>
    <keyword id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword id="KW-0222">Digestion</keyword>
    <keyword id="KW-0372">Hormone</keyword>
    <keyword id="KW-0527">Neuropeptide</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000283082" evidence="3">
        <location>
            <begin position="30"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_0000283083" description="Neuropeptide F" evidence="3">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000283084" evidence="1">
        <location>
            <begin position="66"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phenylalanine amide" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8MP00"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q9VET0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <sequence length="102" mass="11461" checksum="F14493E2EE4A7A16" modified="2009-01-20" version="3" precursor="true">MSNTMRCILIVCVALTLIAAGCNVEASNSRPPRKNDVNTMADAYKFLQDLDTYYGDRARVRFGKRGGPLMEMLRNRELENNMAKSINSGGELIRALDEEEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CN5</accession>
    <accession>A0A6I8UAB6</accession>
    <name>Q29CN5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extensin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4811737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352309.2">
        <property type="nucleotide sequence ID" value="XM_001352273.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29CN5"/>
    <dbReference type="STRING" id="46245.Q29CN5"/>
    <dbReference type="GeneID" id="4811737"/>
    <dbReference type="KEGG" id="dpo:4811737"/>
    <dbReference type="eggNOG" id="ENOG502RTNM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1416508_0_0_1"/>
    <dbReference type="InParanoid" id="Q29CN5"/>
    <dbReference type="OMA" id="PYNQFMP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074151">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29CN5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043691256" evidence="2">
        <location>
            <begin position="27"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352309.2"/>
        </source>
    </evidence>
    <sequence length="167" mass="18874" checksum="D36FCD60B08ED182" modified="2008-10-14" version="2">MKNKIALICILVVLSLDWLYEGSVRADSDSDSSSSSSSSGEHKYKYGKKKNKNKYNYPAYPSPYEYNPYPNPYPQNNYNPYPYNQFMPPYQPPYSPYPYNQFMPPYTPQGTLAPSVGPNPSWNYNGPPNQQPAVPPGQAPQTQPASSVINHSLKVNKEYNEDGHHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DE2</accession>
    <accession>A0A6I8UBP5</accession>
    <name>Q29DE2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein serp</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">serp</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352971.1">
        <property type="nucleotide sequence ID" value="XM_001352935.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DE2"/>
    <dbReference type="SMR" id="Q29DE2"/>
    <dbReference type="STRING" id="46245.Q29DE2"/>
    <dbReference type="GeneID" id="4811936"/>
    <dbReference type="KEGG" id="dpo:4811936"/>
    <dbReference type="eggNOG" id="ENOG502QQP5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022576_1_0_1"/>
    <dbReference type="InParanoid" id="Q29DE2"/>
    <dbReference type="OMA" id="TCDWKDS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076772">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10974">
        <property type="entry name" value="CE4_CDA_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.370:FF:000003">
        <property type="entry name" value="CLUMA_CG003232, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.140.10:FF:000011">
        <property type="entry name" value="CSON009188 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000128">
        <property type="entry name" value="Vermiform, isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052740">
        <property type="entry name" value="CE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45985:SF6">
        <property type="entry name" value="FI03450P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352971.1"/>
        </source>
    </evidence>
    <sequence length="541" mass="61623" checksum="D828E2ECEE1D51B6" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29F87</accession>
    <accession>A0A6I8UET8</accession>
    <name>Q29F87_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyridoxal kinase</fullName>
            <ecNumber evidence="5">2.7.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Pyridoxine kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Pdxk</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyridoxal + ATP = pyridoxal 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17310"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:10225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>pyridoxamine + ATP = pyridoxamine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57761"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:25105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>pyridoxine + ATP = pyridoxine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25108"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16709"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:25109"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxal: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxamine 5'-phosphate from pyridoxamine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxine 5'-phosphate from pyridoxine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001354315.1">
        <property type="nucleotide sequence ID" value="XM_001354279.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29F87"/>
    <dbReference type="FunCoup" id="Q29F87">
        <property type="interactions" value="1170"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29F87"/>
    <dbReference type="GeneID" id="4814189"/>
    <dbReference type="KEGG" id="dpo:4814189"/>
    <dbReference type="eggNOG" id="KOG2599">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046496_1_1_1"/>
    <dbReference type="InParanoid" id="Q29F87"/>
    <dbReference type="OMA" id="HTQYGQW"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00298"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078195">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29F87">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="15">
        <location>
            <begin position="100"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001354315.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="33229" checksum="89D74C2BFA4A3809" modified="2006-04-04" version="1">MASAAATKRVLSIQSHVVHGYVGNKVATYPLQLLGFDVDPLNSVQFSNHTGYKTFKGPISNEKELVTIIEGLEGNELLGHYSHLLTGYIGNPLFLREVGIIVQKLRQANPNLVYVCDPVMGDNGQLYVPKELLPIYRDEIIPLADIITPNQYEVELLTGKEVRSETAVWEAMDWFHKRQIKTVVISSSDLGQPGVLRAFLSQLNGPRLAIDIPKQGGKDLVFTGTGDLFASLFLAHSHACEDVSDVFEKTIASLQAVIKRTVAALPHNDGPVKACERELKLVQSKAEIEKPQVLLRAQRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JH7</accession>
    <accession>A0A6I8UGU3</accession>
    <name>Q29JH7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Setd3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-histidyl-[protein] + S-adenosyl-L-methionine = N(tele)-methyl-L-histidyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19369"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:11600"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16367"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.85"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class V-like SAM-binding methyltransferase superfamily. SETD3 actin-histidine methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001355267.2">
        <property type="nucleotide sequence ID" value="XM_001355231.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JH7"/>
    <dbReference type="FunCoup" id="Q29JH7">
        <property type="interactions" value="2317"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JH7"/>
    <dbReference type="GeneID" id="4815968"/>
    <dbReference type="KEGG" id="dpo:4815968"/>
    <dbReference type="eggNOG" id="KOG1337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028272_1_0_1"/>
    <dbReference type="InParanoid" id="Q29JH7"/>
    <dbReference type="OMA" id="QCFPIQI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077122">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JH7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19176">
        <property type="entry name" value="SET_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000026">
        <property type="entry name" value="Actin-histidine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015353">
        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025785">
        <property type="entry name" value="SETD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044428">
        <property type="entry name" value="SETD3_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF47">
        <property type="entry name" value="ACTIN-HISTIDINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09273">
        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51565">
        <property type="entry name" value="SAM_MT85_SETD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="10"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355267.2"/>
        </source>
    </evidence>
    <sequence length="568" mass="64295" checksum="C77437197F7FF2F5" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LJ2</accession>
    <accession>A0A6I8UKJ2</accession>
    <name>Q29LJ2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4817583</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356987.1">
        <property type="nucleotide sequence ID" value="XM_001356951.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LJ2"/>
    <dbReference type="FunCoup" id="Q29LJ2">
        <property type="interactions" value="1701"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LJ2"/>
    <dbReference type="GeneID" id="4817583"/>
    <dbReference type="KEGG" id="dpo:4817583"/>
    <dbReference type="eggNOG" id="KOG2946">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059592_3_0_1"/>
    <dbReference type="InParanoid" id="Q29LJ2"/>
    <dbReference type="OMA" id="IKFYHVL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077600">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356987.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25208" checksum="A0666821C29E0131" modified="2006-04-04" version="1">MDAKLDMFEDVNTSPSPSLEGDMSIPGKRTTTTTSQNGVPDYNTLDEPIKETVLRDIRAVGVKFYHVLYPKEKSSLLRDWDLWGPLVLCTFMATILQGSSSADSMSDNGPEFAQVFVIVWIGAAIVTLNSKLLGGNISFFQSVCVLGYCLTPVAISLIVCRIILLSTQTRLLFFLRFVTTTMGFAWATYASFIFLGQSQPPHRKPLAVYPIFLFFFIISWLVLSHN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PG5</accession>
    <name>MOC32_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenylyltransferase and sulfurtransferase MOCS3-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Molybdenum cofactor synthesis protein 3-2</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="2">Molybdopterin-synthase adenylyltransferase 2</fullName>
                <ecNumber evidence="2">2.7.7.80</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">Adenylyltransferase MOCS3-2</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="2">Sulfur carrier protein MOCS2A adenylyltransferase 2</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="2">Molybdopterin-synthase sulfurtransferase 2</fullName>
                <ecNumber evidence="2">2.8.1.11</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2">Sulfur carrier protein MOCS2A sulfurtransferase 2</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="2">Sulfurtransferase MOCS3-2</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name type="ORF">GA12041</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:43616"/>
            <dbReference type="Rhea" id="RHEA-COMP:12159"/>
            <dbReference type="Rhea" id="RHEA-COMP:12202"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="EC" id="2.7.7.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [molybdopterin-synthase sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48612"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12159"/>
            <dbReference type="Rhea" id="RHEA-COMP:19907"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.80" evidence="2"/>
    <dbReference type="EC" id="2.8.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CH379058">
        <property type="protein sequence ID" value="EAL34328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PG5"/>
    <dbReference type="SMR" id="Q29PG5"/>
    <dbReference type="FunCoup" id="Q29PG5">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29PG5"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0280683">
        <property type="protein sequence ID" value="FBpp0279121"/>
        <property type="gene ID" value="FBgn0072089"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817985"/>
    <dbReference type="eggNOG" id="KOG2017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_1_2_1"/>
    <dbReference type="InParanoid" id="Q29PG5"/>
    <dbReference type="OMA" id="IPDVGMD"/>
    <dbReference type="PhylomeDB" id="Q29PG5"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072089">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061605">
        <property type="term" value="F:molybdopterin-synthase adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061604">
        <property type="term" value="F:molybdopterin-synthase sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042292">
        <property type="term" value="F:URM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000014">
        <property type="entry name" value="Adenylyltransferase and sulfurtransferase MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000206">
        <property type="entry name" value="Adenylyltransferase and sulfurtransferase MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03049">
        <property type="entry name" value="MOCS3_Uba4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028885">
        <property type="entry name" value="MOCS3/Uba4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004281">
        <property type="entry name" value="PRK05690.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000369208" description="Adenylyltransferase and sulfurtransferase MOCS3-2">
        <location>
            <begin position="1"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="353"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate; for adenylyltransferase activity" evidence="2">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate; for sulfurtransferase activity" evidence="2">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="127"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphothreonine" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="451" mass="50086" checksum="31E6FDE5E76538E3" modified="2006-04-04" version="1">MIDSEALESERVKLKRDIADLRANLNRKEQCLRELEAAIAAGEDSDEAEESSNDMPTPQTKLTNDDIARYSRQLILQDFGVQGQLKLKNSSVLIVGMGGLGCPAAQYLVAAGCGHLGLIDYDEVERSNLHRQILHSEHRCGMSKAESARIALLELNSHCQIRCHSRLINSMNAMHIIRPYDVVLDCSDNVATRYLLNDACVMLRKPLVSGSALKMDGQLTVYGYGQGPCYRCIYPVPPPPEAVTNCGDGGVLGAVTGIIGAMQALEAIKVIIGLGDVMSGRLLIFDGSSFMFRNIRIRTKRPNCHVCSAQPLITELIDYEMFCGMHATDKDNPLDLLEPDQRLEVKEYHQKLQSQPHLLLDVRPPAEFEICQLPRSINVPLSEILDDSYLKRFAKQLEDKELPIVLLCRRGNDSQIAVQHITNRFPAHSIRDLVGGLHAWTGSVDATFPIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LYK1</accession>
    <name>LIAS_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoyl synthase, mitochondrial</fullName>
            <ecNumber evidence="1">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lipoate synthase</fullName>
            <shortName evidence="1">LS</shortName>
            <shortName evidence="1">Lip-syn</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">Las</name>
        <name type="ORF">GA18753</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">This protein may be expected to contain an N-terminal transit peptide but none has been predicted.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CH379069">
        <property type="protein sequence ID" value="EAL29861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001354122.1">
        <property type="nucleotide sequence ID" value="XM_001354086.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LYK1"/>
    <dbReference type="SMR" id="Q2LYK1"/>
    <dbReference type="FunCoup" id="Q2LYK1">
        <property type="interactions" value="1844"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LYK1"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0277155">
        <property type="protein sequence ID" value="FBpp0275593"/>
        <property type="gene ID" value="FBgn0078753"/>
    </dbReference>
    <dbReference type="GeneID" id="4813963"/>
    <dbReference type="KEGG" id="dpo:4813963"/>
    <dbReference type="CTD" id="40259"/>
    <dbReference type="eggNOG" id="KOG2672">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_2_1_1"/>
    <dbReference type="InParanoid" id="Q2LYK1"/>
    <dbReference type="OMA" id="PYCDIDF"/>
    <dbReference type="PhylomeDB" id="Q2LYK1"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078753">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000036">
        <property type="entry name" value="Lipoyl synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004019">
        <property type="entry name" value="PRK05481.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009544">
        <property type="entry name" value="PRK12928.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="LIPOYL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949:SF0">
        <property type="entry name" value="LIPOYL SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01058">
        <property type="entry name" value="lipoyl_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000398221" description="Lipoyl synthase, mitochondrial">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="117"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="374" mass="42118" checksum="E27E02B8B2B5A9F7" modified="2006-02-21" version="1">MLTAFRPNSPTAIVVRAASTNAKKLEEIRERLAKGPNFQDFVQNPDNLKSVGENYDGKLRREKGEEQRLRLPPWLKTTIPMGKNYTKIKEQLRELKLSTVCEEARCPNIGECWGGGENGTQTATIMLMGDTCTRGCRFCSVKTARIPPPLDENEPVNTAKAIASWGLDYIVLTSVDRDDLPDGGSKHIAQTVREIKARNSNIFVECLVPDFRGDLDCVETIANCGLDVYAHNIETVEKLTPYVRDRRAHYRQTLRVLTEAKRFNPNLITKSSIMLGLGETDEEIERTMTDLREAGVECLTLGQYMQPTNKHLKVIEYVTPEKFKHWEERGNDLGFLYTASGPLVRSSYKAGEFFISSILANRKANQSKDSAPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ34</accession>
    <accession>A0A6I8UDW6</accession>
    <name>Q2LZ34_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353939.2">
        <property type="nucleotide sequence ID" value="XM_001353903.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ34"/>
    <dbReference type="SMR" id="Q2LZ34"/>
    <dbReference type="GeneID" id="4812850"/>
    <dbReference type="KEGG" id="dpo:4812850"/>
    <dbReference type="eggNOG" id="ENOG502S0MZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082525_1_0_1"/>
    <dbReference type="InParanoid" id="Q2LZ34"/>
    <dbReference type="OMA" id="MYTYADP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353939.2"/>
        </source>
    </evidence>
    <sequence length="236" mass="27248" checksum="583556C311DAC7B3" modified="2008-10-14" version="2">MSKLDDLLAKKKTVVPVLDLSRSNIQTSEEFKRLFEKVPDYKMRPVKVRAEEIKLRDKDYAFKMPKKEMRKLLKSNGEREPLYSYADPVPTEMKDVVLMELCAVPIDWKMLTTLRPKNKQEEEYFSRMVEMGKLELKTEARDRREFALNNCVKKIKNKSGIVETRLMTCESCGEEMCCGKSCGDFNYDLYIRVEARVVKPKPVPMTTNKTKLNGRKKSSVAGTKIKVKKSSTNKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ73</accession>
    <accession>A0A6I8UDZ8</accession>
    <name>Q2LZ73_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Major facilitator superfamily domain-containing protein 6-like protein A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Sugb</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353900.2">
        <property type="nucleotide sequence ID" value="XM_001353864.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ73"/>
    <dbReference type="FunCoup" id="Q2LZ73">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="GeneID" id="4813877"/>
    <dbReference type="KEGG" id="dpo:4813877"/>
    <dbReference type="eggNOG" id="KOG3762">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013133_2_2_1"/>
    <dbReference type="InParanoid" id="Q2LZ73"/>
    <dbReference type="OMA" id="KMWGSIG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080265">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZ73">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17335">
        <property type="entry name" value="MFS_MFSD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF30">
        <property type="entry name" value="SUGAR BABY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="367"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="559"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="568"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="581"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353900.2"/>
        </source>
    </evidence>
    <sequence length="604" mass="66808" checksum="868B54769215EC2C" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZC1</accession>
    <accession>A0A6I8UDN6</accession>
    <name>Q2LZC1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine protease 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Jon65Aiv</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353851.2">
        <property type="nucleotide sequence ID" value="XM_001353815.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZC1"/>
    <dbReference type="SMR" id="Q2LZC1"/>
    <dbReference type="MEROPS" id="S01.B05"/>
    <dbReference type="GeneID" id="5495512"/>
    <dbReference type="KEGG" id="dpo:5495512"/>
    <dbReference type="eggNOG" id="KOG3627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006842_7_6_1"/>
    <dbReference type="InParanoid" id="Q2LZC1"/>
    <dbReference type="OMA" id="ISNTKCA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079614">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000025">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="39"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353851.2"/>
        </source>
    </evidence>
    <sequence length="274" mass="28413" checksum="A645EF0D4CEE2246" modified="2008-10-14" version="2">MKFLIILALAIASASAFPEPELVSRNRVMPVQDSIEGRITGGSAATTGQFPYQVGLSLKLSALSSAWCGGSLIGNQWVLTAAHCTDGVQSVTVYLGATVRTSAEVTHTVASSQILIHSGWNSANLRNDISLIKIPVTSYSTKIQAARIPAIASSYSTYAGDSVIASGWGKISDSATGVTNNLQWAKLTVITNSVCANTYGTSIVTASNICVSTPNGVSTCNGDSGGPLVLESSKVQVGLTSFGAAAGCQKGYPAAFTRLTSYLDWIKTNTGISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZX0</accession>
    <accession>A0A6I8UD90</accession>
    <name>Q2LZX0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Unc-112-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Fit1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the kindlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353653.1">
        <property type="nucleotide sequence ID" value="XM_001353617.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZX0"/>
    <dbReference type="SMR" id="Q2LZX0"/>
    <dbReference type="FunCoup" id="Q2LZX0">
        <property type="interactions" value="315"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZX0"/>
    <dbReference type="GeneID" id="4813063"/>
    <dbReference type="KEGG" id="dpo:4813063"/>
    <dbReference type="eggNOG" id="KOG3727">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011611_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LZX0"/>
    <dbReference type="OMA" id="PEHGIHY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073445">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13205">
        <property type="entry name" value="FERM_C_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17095">
        <property type="entry name" value="FERM_F0_kindlins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17096">
        <property type="entry name" value="FERM_F1_kindlins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01237">
        <property type="entry name" value="PH_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000037">
        <property type="entry name" value="Fermitin family homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000502">
        <property type="entry name" value="Unc-112-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000253">
        <property type="entry name" value="unc-112-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040790">
        <property type="entry name" value="Kindlin_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037837">
        <property type="entry name" value="PH_Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF13">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18124">
        <property type="entry name" value="Kindlin_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
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        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353653.1"/>
        </source>
    </evidence>
    <sequence length="711" mass="80836" checksum="10E6000917576E34" modified="2006-02-21" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0J9</accession>
    <name>KLHDB_DROPS</name>
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        <recommendedName>
            <fullName evidence="1">Kelch-like protein diablo</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="primary">dbo</name>
        <name type="ORF">GA19454</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. May have a role in synapse differentiation and growth.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="CH379069">
        <property type="protein sequence ID" value="EAL30933.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_015187346.1"/>
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    <dbReference type="RefSeq" id="XP_015042833.1">
        <property type="nucleotide sequence ID" value="XM_015187347.1"/>
    </dbReference>
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        <property type="interactions" value="1900"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0J9"/>
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        <property type="protein sequence ID" value="FBpp0329308"/>
        <property type="gene ID" value="FBgn0079450"/>
    </dbReference>
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        <property type="protein sequence ID" value="FBpp0339605"/>
        <property type="gene ID" value="FBgn0079450"/>
    </dbReference>
    <dbReference type="GeneID" id="4813272"/>
    <dbReference type="KEGG" id="dpo:4813272"/>
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        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
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        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of synaptic assembly at neuromuscular junction"/>
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        <property type="project" value="UniProtKB"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BACK"/>
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        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
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        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
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        <property type="entry name" value="KELCH PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactose oxidase, central domain"/>
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    </dbReference>
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        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0009">Actin-binding</keyword>
    <keyword id="KW-0880">Kelch repeat</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="chain" id="PRO_0000379951" description="Kelch-like protein diablo">
        <location>
            <begin position="1"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="74"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="BACK" evidence="3">
        <location>
            <begin position="176"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 1" evidence="3">
        <location>
            <begin position="325"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 2" evidence="3">
        <location>
            <begin position="373"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 3" evidence="3">
        <location>
            <begin position="420"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 4" evidence="3">
        <location>
            <begin position="468"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 5" evidence="3">
        <location>
            <begin position="515"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 6" evidence="3">
        <location>
            <begin position="561"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9VUU5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9Y2M5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EAL30933.2"/>
        </source>
    </evidence>
    <sequence length="628" mass="69197" checksum="C4CE0AF05E471E23" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NWP0</accession>
    <accession>A0A6I8VMH7</accession>
    <name>A0A0R3NWP0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tau-tubulin kinase homolog Asator isoform X2</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein, isoform C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Dpse\GA11055</name>
        <name evidence="12" type="synonym">Asator</name>
        <name evidence="10" type="ORF">Dpse_GA11055</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
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                <person name="Wong G.K."/>
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                <person name="Wu G."/>
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                <person name="Yang H.P."/>
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                <person name="Patti C."/>
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                <person name="Sherpa N."/>
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                <person name="Shih D."/>
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                <person name="Tsamla T."/>
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                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
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                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
                <person name="Yu Q."/>
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            <dbReference type="PubMed" id="17994087"/>
            <dbReference type="DOI" id="10.1038/nature06341"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
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    <reference evidence="10" key="3">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
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                <person name="English A.C."/>
                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
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            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
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        <text evidence="6">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KRT05496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015044539.1"/>
        </source>
    </evidence>
    <sequence length="1453" mass="158976" checksum="A0E9412E4507721A" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P164</accession>
    <accession>A0A6I8VAW2</accession>
    <name>A0A0R3P164_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">3,4-dihydroxyphenylacetaldehyde synthase 2 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">amd</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015036857.1">
        <property type="nucleotide sequence ID" value="XM_015181371.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P164"/>
    <dbReference type="SMR" id="A0A0R3P164"/>
    <dbReference type="FunCoup" id="A0A0R3P164">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P164"/>
    <dbReference type="GeneID" id="4816707"/>
    <dbReference type="InParanoid" id="A0A0R3P164"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0046148">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004058">
        <property type="term" value="F:aromatic-L-amino-acid decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF60">
        <property type="entry name" value="3,4-DIHYDROXYPHENYLACETALDEHYDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015036857.1"/>
        </source>
    </evidence>
    <sequence length="510" mass="56891" checksum="1FA9A43AD8A6DDF6" modified="2016-01-20" version="1">MDAKEFREFGKAAVDFVADYLENIRDHEVLPSVEPGYLLDLLPKDMPEEPEHWTEVLSDINRVIKPGITHWQSPNMHGYYPTSTSYPSIVGEMLASGFGIIGFSWICSPACTELEVVVMDWLAKFLKLPEHFQHASDGPGGGVIQGSASEAVLVAVLAAREQAVISCKETHPELSESEVRGKLIAYSSDQSNSCIEKAGVLAAMPIKLLPADDDLILRGNTLRKAIEDDVAAGLIPVICIATLGTTGTCAYDDIDSLADVCQALNVWLHVDAAYAGGGFALEECTELRRGLDRVDSLNFNLHKFMLVNFDCSAMWLKDANKVVDSFNVDRIYLKHKHEGQSQIPDFRHWQIPLGRRFRALKVWITFRTLGAEGLRSHVRKHIALAQQFEAFVRNDSRFEMVAPQALGLVCFRPRGDNEHTAQLLQRLMERKKIYMVKAEHAGRQFLRFAVCGMDAKPSDIEFAWTEIETQLTALLAEKSLAARKPSNVSELTQHFQMHLASDSGTHEKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P3X8</accession>
    <accession>A0A6I8VH85</accession>
    <name>A0A0R3P3X8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dihydropyrimidine dehydrogenase [NADP(+)]</fullName>
            <shortName evidence="18">DHPDHase</shortName>
            <shortName evidence="18">DPD</shortName>
            <ecNumber evidence="18">1.3.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Dihydrothymine dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Dihydrouracil dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">su(r)</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>5,6-dihydrouracil + NADP(+) = uracil + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18093"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15901"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 4 [4Fe-4S] clusters. Contains approximately 16 iron atoms per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Amino-acid biosynthesis; beta-alanine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the dihydropyrimidine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.2" evidence="18"/>
    <dbReference type="RefSeq" id="XP_015041308.1">
        <property type="nucleotide sequence ID" value="XM_015185822.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P3X8"/>
    <dbReference type="FunCoup" id="A0A0R3P3X8">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P3X8"/>
    <dbReference type="GeneID" id="4815980"/>
    <dbReference type="eggNOG" id="KOG1799">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3P3X8"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="UniPathway" id="UPA00131"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075315">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P3X8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017113">
        <property type="term" value="F:dihydropyrimidine dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002058">
        <property type="term" value="F:uracil binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019483">
        <property type="term" value="P:beta-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006212">
        <property type="term" value="P:uracil catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02940">
        <property type="entry name" value="DHPD_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000007">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000023">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000061">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073:SF2">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 18" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12 18" id="KW-0274">FAD</keyword>
    <keyword evidence="7 18" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 18" id="KW-0288">FMN</keyword>
    <keyword evidence="15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 18" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU364041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015041308.1"/>
        </source>
    </evidence>
    <sequence length="1067" mass="115512" checksum="382E153775C76ABF" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P637</accession>
    <accession>A0A6I8WBP5</accession>
    <name>A0A0R3P637_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Sodium-independent sulfate anion transporter isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">LOC4811915</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
            <tissue evidence="9 10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015043724.1">
        <property type="nucleotide sequence ID" value="XM_015188238.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043726.1">
        <property type="nucleotide sequence ID" value="XM_015188240.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240830.1">
        <property type="nucleotide sequence ID" value="XM_033384939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P637"/>
    <dbReference type="GeneID" id="4811915"/>
    <dbReference type="KEGG" id="dpo:4811915"/>
    <dbReference type="eggNOG" id="KOG0236">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="OMA" id="TGPMSVT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079957">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000043">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="420"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="6">
        <location>
            <begin position="27"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="474"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015043724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015043726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033240830.1"/>
        </source>
    </evidence>
    <sequence length="617" mass="65432" checksum="2BF6B9EC4C1D699B" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCC8</accession>
    <name>A0A6I8UCC8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Carboxypeptidase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4813344</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353235.2">
        <property type="nucleotide sequence ID" value="XM_001353199.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCC8"/>
    <dbReference type="FunCoup" id="A0A6I8UCC8">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813344"/>
    <dbReference type="InParanoid" id="A0A6I8UCC8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCC8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.340:FF:000006">
        <property type="entry name" value="GD14014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF140">
        <property type="entry name" value="FI02848P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026087157" evidence="12">
        <location>
            <begin position="18"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="122"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001353235.2"/>
        </source>
    </evidence>
    <sequence length="418" mass="46349" checksum="907B2955C0F9A2B1" modified="2020-08-12" version="1">MLKSFAFLLLLCGTALAADSYDGYKIYDINTRTVFEKELLLRLSRNADLDFFDLPRSLEAASRVMVQPKDQVGFEQLLQKFGVEYSVVDQDLGVSLRQEQLENQSQRLMAQRSTARSISFSAFHRHSEINAYLDELAAAYPSRVSVQVAGKSYENRDIKTITISNGDGKTGKSVIFLDAGIHAREWIAHAGALYVIHQLVENFAANSALLKNYDWVILPVVNPDGYEYTHTSTRMWRKTRKPASASCYGTDANRNFDYHWGEVGASSYSCDDTFKGQTAFSEPETQLVRDLLLNLKGRGKFYLTLHSYGNYLLYPYGWTSALPASWRDIDNVAQAGAAAIKASTGTKYTVGSSTNVLYAAAGGSDDYAFAVAEFPISITMELPAGGTGFNPTTAQILPYVDETWTGIKAMAQKVVENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCG3</accession>
    <name>A0A6I8UCG3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD repeat-containing protein 63</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mmm</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353254.3">
        <property type="nucleotide sequence ID" value="XM_001353218.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCG3"/>
    <dbReference type="FunCoup" id="A0A6I8UCG3">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813198"/>
    <dbReference type="InParanoid" id="A0A6I8UCG3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036156">
        <property type="term" value="C:inner dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF5">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="884"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="884"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353254.3"/>
        </source>
    </evidence>
    <sequence length="1138" mass="131300" checksum="A4E20B44E34EB72E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UD80</accession>
    <name>A0A6I8UD80_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mpv17-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4813352</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353682.4">
        <property type="nucleotide sequence ID" value="XM_001353646.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UD80"/>
    <dbReference type="FunCoup" id="A0A6I8UD80">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813352"/>
    <dbReference type="InParanoid" id="A0A6I8UD80"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UD80">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061668">
        <property type="term" value="P:mitochondrial ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF81">
        <property type="entry name" value="GH12661P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001353682.4"/>
        </source>
    </evidence>
    <sequence length="228" mass="26651" checksum="F3197B5FB05A29E1" modified="2020-08-12" version="1">MWHIVSTAPMRNAVSRLLIHQKHPATVLATSLPRNYGRGRHWNNFLAKMFGKYLAVTNIVGSGLLLVAGDAVTQQYERAVHKRLFDFHRSGCMFLTGLAVGPVQHAFYSHLDEYLPDSKRITAVKKIFFDQMFMSPTYIFLFFYVSSLLEGKTIRESNAEIREKFLYTWMIDCLIWPAVQYLNFRFLNPRHRVVFINVTNCMYIVLLSYIKHDWALHNQNPEIKEKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDE8</accession>
    <name>A0A6I8UDE8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein Ir67c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ir67c</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353773.3">
        <property type="nucleotide sequence ID" value="XM_001353737.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDE8"/>
    <dbReference type="FunCoup" id="A0A6I8UDE8">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812892"/>
    <dbReference type="InParanoid" id="A0A6I8UDE8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF39">
        <property type="entry name" value="IONOTROPIC RECEPTOR 56A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026167224" evidence="9">
        <location>
            <begin position="17"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353773.3"/>
        </source>
    </evidence>
    <sequence length="563" mass="64237" checksum="B067C8B39E33840A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDY3</accession>
    <name>A0A6I8UDY3_DROPS</name>
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        <recommendedName>
            <fullName evidence="1">gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">LOC4813554</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.2.9" evidence="1"/>
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        <property type="nucleotide sequence ID" value="XM_001353947.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043370.2">
        <property type="nucleotide sequence ID" value="XM_015187884.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDY3"/>
    <dbReference type="KEGG" id="dpo:4813554"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDY3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017939">
        <property type="entry name" value="G-Glutamylcylcotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935:SF0">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13772">
        <property type="entry name" value="AIG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001353983.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015043370.2"/>
        </source>
    </evidence>
    <sequence length="186" mass="20689" checksum="52D90AD5752A33EC" modified="2020-08-12" version="1">MACKFYYFGFGSNMLARRIHIQNPTAKRIGAGKLENYRLDFHTGSKNWLGAPATIVPTAGAHVYGAIWEIDMCNLKDLDDQESVPDGVYIPISVPVLSLTSNTDIICRAYHLTNQPLSEVHGQSVPPPSIPLDRQPSQTYLKVLVKGALETGIPEEYVKWLRGLKHNGNQVATMEEKLQLDQVQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEA2</accession>
    <name>A0A6I8UEA2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HECT-type E3 ubiquitin transferase NEDD4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Nedd4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001354134.4">
        <property type="nucleotide sequence ID" value="XM_001354098.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEA2"/>
    <dbReference type="FunCoup" id="A0A6I8UEA2">
        <property type="interactions" value="1152"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813977"/>
    <dbReference type="InParanoid" id="A0A6I8UEA2"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UEA2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008586">
        <property type="term" value="P:imaginal disc-derived wing vein morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04033">
        <property type="entry name" value="C2_NEDD4_NEDD4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000037">
        <property type="entry name" value="E3 ubiquitin-protein ligase nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000096">
        <property type="entry name" value="E3 ubiquitin-protein ligase Nedd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000021">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="55"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="250"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="551"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="602"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="694"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="18"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="306"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="435"/>
            <end position="557"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="580"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="507"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001354134.4"/>
        </source>
    </evidence>
    <sequence length="1029" mass="117344" checksum="9970AC80C5AEF29B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEU1</accession>
    <name>A0A6I8UEU1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">YLP motif-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Nuclear protein ZAP3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18 19" type="primary">ZAP3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18 19">MV-25-SWS-2005</strain>
            <tissue evidence="18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in the reduction of telomerase activity during differentiation of embryonic stem cells by binding to the core promoter of TERT and controlling its down-regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with PPP1CA and NCOA5. Forms a complex with ILF2, ILF3, KHDRBS1, RBMX, NCOA5 and PPP1CA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354419.3">
        <property type="nucleotide sequence ID" value="XM_001354383.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240389.1">
        <property type="nucleotide sequence ID" value="XM_033384498.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814248"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UEU1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032204">
        <property type="term" value="P:regulation of telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000399">
        <property type="entry name" value="YLP motif containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058903">
        <property type="entry name" value="Spectrin_YLPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026314">
        <property type="entry name" value="YLP_motif_con_p1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413">
        <property type="entry name" value="YLP MOTIF CONTAINING PROTEIN NUCLEAR PROTEIN ZAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413:SF0">
        <property type="entry name" value="YLP MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26583">
        <property type="entry name" value="Spectrin_YLPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="YLPM1-like spectrin repeat" evidence="16">
        <location>
            <begin position="680"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="213"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1072"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1559"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1697"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="289"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="328"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="449"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="514"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="551"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="589"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="638"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="695"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="720"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="818"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="888"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="949"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1089"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1132"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1150"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1172"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1224"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1246"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1259"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1288"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1362"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1487"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1580"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1632"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1650"/>
            <end position="1661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF26583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001354419.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033240389.1"/>
        </source>
    </evidence>
    <sequence length="2168" mass="228394" checksum="2F9CD3165E64A3B9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEY6</accession>
    <name>A0A6I8UEY6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">NAD(P)H-hydrate epimerase</fullName>
            <ecNumber evidence="3 10">5.1.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(P)HX epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Naxe</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="10">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.99.6" evidence="3 10"/>
    <dbReference type="RefSeq" id="XP_001354421.4">
        <property type="nucleotide sequence ID" value="XM_001354385.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEY6"/>
    <dbReference type="FunCoup" id="A0A6I8UEY6">
        <property type="interactions" value="1400"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814332"/>
    <dbReference type="InParanoid" id="A0A6I8UEY6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13232">
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    </dbReference>
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        <property type="entry name" value="NAD(P)H-HYDRATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="11">
        <location>
            <begin position="72"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="120"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="189"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001354421.4"/>
        </source>
    </evidence>
    <sequence length="288" mass="32784" checksum="941491A6DAC59964" modified="2020-08-12" version="1">MRLPSWQCLRWNGCIPRTCQLLWRRKNTQSRLPFSPFAIIEKQQQRQNQQLKRHFSLQSCKNMVKYLSQSEAIELDLDLFEMFHVGQLMELAGLSCADAVAECFPAQTHPRVLVVCGPGNNGGDGLVCARHLSLMGYQPTVYYPKPTLMSLYENLTNQCHHMEIPSVKKCPSVSDAEEDYDLILDALFGFGFKPPVREDFVPLVKMMQETKVPIASVDIPSGWDVEKGKQSECDFEPKLLISLTAPKLCAEHFKGEHHYLGGRFVPPALQRKYQLNLPDYGSKLVVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFI2</accession>
    <name>A0A6I8UFI2_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Probable G-protein coupled receptor Mth-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mthl1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354694.3">
        <property type="nucleotide sequence ID" value="XM_001354658.4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="dpo:4815301"/>
    <dbReference type="InParanoid" id="A0A6I8UFI2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFI2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15039">
        <property type="entry name" value="7tmB3_Methuselah-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052808">
        <property type="entry name" value="GPCR_Mth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46953">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46953:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026178184" evidence="7">
        <location>
            <begin position="23"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="431"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="547"/>
            <end position="568"/>
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        <location>
            <begin position="574"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="338"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
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        <location>
            <begin position="651"/>
            <end position="692"/>
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    </feature>
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        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
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        <location>
            <begin position="651"/>
            <end position="679"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001354694.3"/>
        </source>
    </evidence>
    <sequence length="692" mass="75098" checksum="4386938292FC29A5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFN2</accession>
    <name>A0A6I8UFN2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4814970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354728.4">
        <property type="nucleotide sequence ID" value="XM_001354692.4"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:4814970"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026040421" evidence="2">
        <location>
            <begin position="23"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354728.4"/>
        </source>
    </evidence>
    <sequence length="339" mass="37609" checksum="56260FB8B1D61089" modified="2020-08-12" version="1">MTSNRIWLVLPLAMMLATLILAKSSVQAAPVEEVDVNVDKGQSQSQSQNQLSSTLEKDRQRIDCKLTFDAATMGSLGCHDEMDMGQEVEAAVAESGASSTANKPVQLQRHLKSPDSQPIYELQLVVWPSDIEDGDDFNPLEDSTTTTTTTTKPTPTTTTTPTPTPTPTTTITPPTTTTATQIWPLYEDQLAVFPQMDFEEENLDYFLDEPVPAPAPPSISSTTARPRPIDPTGTTTPTPTYVVEHYHVIHPNGTEEYKLVLSNGLANYKKLYTKRVGEQLINVQEGYNSVPIAGRRNLIQTQYFIADERGYNVYKIEQNYHQPGLPKHLHYKATKPSNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFV6</accession>
    <name>A0A6I8UFV6_DROPS</name>
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        <recommendedName>
            <fullName evidence="5">Venom allergen-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4814732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354820.2">
        <property type="nucleotide sequence ID" value="XM_001354784.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFV6"/>
    <dbReference type="FunCoup" id="A0A6I8UFV6">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814732"/>
    <dbReference type="InParanoid" id="A0A6I8UFV6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFV6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1754"/>
            <end position="1769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1984"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2514"/>
            <end position="2524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="3115"/>
            <end position="3136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="3601"/>
            <end position="3610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355573.4"/>
        </source>
    </evidence>
    <sequence length="3630" mass="402029" checksum="1A31500ADE3D0872" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK63</accession>
    <name>A0A6I8UK63_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleoporin NUP42</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleoporin-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4817357</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356835.3">
        <property type="nucleotide sequence ID" value="XM_001356799.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK63"/>
    <dbReference type="FunCoup" id="A0A6I8UK63">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817357"/>
    <dbReference type="InParanoid" id="A0A6I8UK63"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK63">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051767">
        <property type="entry name" value="Nucleoporin_NUP42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="277"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001356835.3"/>
        </source>
    </evidence>
    <sequence length="379" mass="41890" checksum="E390EC5B8A7C0E6F" modified="2020-08-12" version="1">MVVCRFFQQGSCRFGTKCSNEHFDIKVVLKNDMEASINGKMWPFSVYGPFKDKANIPNFIEDQSFEEVRLLCYEAKRLNSFDQFHQQFNREVMEATNKMKTMIQVTPQMVDVMIKIYDAPEGASAVSSAPNQNPFAATASNTAGSIFSKPTLGNQVNNTQSLFGSSMASANSGNSIFGGGSVAGATSVFGQPQQQQQQQGAGPQTFQGGNIFAQAQAQPPNQMPFGGFQQQQPGGVFGQAAVNPSGGIFGQVAQQPQQPPSGLFAQAAQTVGFPQQHQQQQQQAQQMMQQVNTQQQQQLLQQQQQQMMQQQQQHQLMQQQQHQQQQQQQQQQQQQQSQPGGSSVYSLLESLTPAEIEAFKADQFLPGHVPFNPPPREFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKY2</accession>
    <name>A0A6I8UKY2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lopap</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4817628</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357024.4">
        <property type="nucleotide sequence ID" value="XM_001356988.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKY2"/>
    <dbReference type="FunCoup" id="A0A6I8UKY2">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817628"/>
    <dbReference type="InParanoid" id="A0A6I8UKY2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKY2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022271">
        <property type="entry name" value="Lipocalin_ApoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612:SF34">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036893">
        <property type="entry name" value="Lipocalin_ApoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="4">
        <location>
            <begin position="71"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001357024.4"/>
        </source>
    </evidence>
    <sequence length="218" mass="24940" checksum="E5F7B81464DC0788" modified="2020-08-12" version="1">MANPFSQDPPFHSIPIHSDSNLCIMIMPQLFVFPIIVLIYIPPTLAMIALEGPCPSNMTAVGDLDMDRFLGKWYTYSVYPTFSKRVPKCQTVEFKKEEDKIWIKRTELSTKTDTLKIKVEEIRNVDPSHGKYDLITTNSAFPEGIQINVLDTDYDNFAIRYMCFDSNNLFSFHWAVIQTRIRLPTSDIIYTAQVLARKSGIVLSKMIKIPQHSCPPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL66</accession>
    <name>A0A6I8UL66_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4817903</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357222.4">
        <property type="nucleotide sequence ID" value="XM_001357186.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL66"/>
    <dbReference type="FunCoup" id="A0A6I8UL66">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817903"/>
    <dbReference type="InParanoid" id="A0A6I8UL66"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UL66">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026095">
        <property type="entry name" value="Myb/SANT-like_DNA-bd_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22666">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22666:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="12"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="253"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357222.4"/>
        </source>
    </evidence>
    <sequence length="312" mass="36624" checksum="B11395A73FED72FC" modified="2020-08-12" version="1">MSGGKHMRLRYYTSMEEVTMVRVWREFLDLIPSYSENLPIFRDISHSMQQCGIRLNKQEVRRRINSYRNKYLTERSRVEGDPEYTPEWRLYQIVDCLFNPERPSVDIHLVQNVLESAAMQVRSEHPDLPNIPSSMSRAPYVKFNRDPDGCAFLEGPSPTPPQQTQNLPEMLHFSQNPVKTEPKPLDADEADSPTYAESAIRRTPLMPANYQLQLPVEERMEMGANGQMEYVGKKKRGRRSILPRVGQISLAQLEALRMQNGELERQNENSQMELEFKERQYNELEQTLDLWMHQQEMFLLHFERLGFKTAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMF3</accession>
    <name>A0A6I8UMF3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">stg</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MPI phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001357739.1">
        <property type="nucleotide sequence ID" value="XM_001357702.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMF3"/>
    <dbReference type="FunCoup" id="A0A6I8UMF3">
        <property type="interactions" value="547"/>
    </dbReference>
    <dbReference type="GeneID" id="4800469"/>
    <dbReference type="KEGG" id="dpo:4800469"/>
    <dbReference type="InParanoid" id="A0A6I8UMF3"/>
    <dbReference type="OMA" id="LYTHEQI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072694">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009794">
        <property type="term" value="P:regulation of mitotic cell cycle, embryonic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01530">
        <property type="entry name" value="Cdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000036">
        <property type="entry name" value="M-phase inducer phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF76">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="9">
        <location>
            <begin position="334"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357739.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="56028" checksum="C4AEEB3E57EF8F79" modified="2020-08-12" version="1">MLWETIEEESSSNNCSISSLDCNISNSSNKHNSRRARRSLACSEMMAMDAEELSFYDDDVLPQQRVASPELMGLLSPEGSPQHFQIVRQQKTLPATTEESSTAATPARSFRVFNSLSSTCSMESNMDDEYMELFEMESLAGQQPSSLGFPSGLNSLISGQIKVASPARPIRSPALTMRRPSVRRCLSMTETNSNQLQQLQPQPKTPEILKETARDCFKRPEPPASASCSPIQSKRHRCAEKENCPTPTATVPHSHSSTTTTVTSHPPPLRKCMSLNDAEIMSAMARSENRNEPELIGDFSKTYALPLMEGRHRDLKSISSNTVARLLRGEFAGDVASYRIIDCRYPYEFEGGHIEGAKNLYTTEQILEEFLSVAQTELQQQQASEAGPKRHIIIFHCEFSSERGPKMSRFLRNLDRERNTNAYPALHYPEIYLLHNGYKEFFETHIEHCEPHTYRPMLDPAYNEAYRHFRAKSKSWNGDGLGGATGRLKKSRSRLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMI1</accession>
    <name>A0A6I8UMI1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="4 14">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">P5cr</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4 14"/>
    <dbReference type="RefSeq" id="XP_001357778.2">
        <property type="nucleotide sequence ID" value="XM_001357741.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMI1"/>
    <dbReference type="FunCoup" id="A0A6I8UMI1">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800513"/>
    <dbReference type="InParanoid" id="A0A6I8UMI1"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UMI1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000190">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF69">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 14" id="KW-0521">NADP</keyword>
    <keyword evidence="10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 14" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="15">
        <location>
            <begin position="7"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="16">
        <location>
            <begin position="173"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001357778.2"/>
        </source>
    </evidence>
    <sequence length="280" mass="29693" checksum="134195995F304D74" modified="2020-08-12" version="1">MAKLDERIGFIGGGNMAFAIGSGLVRCAIVKPSQVLVSGPHIKNLSRWRELGAVTTDDNCEVLEHTDVVFICVKPHILGQCASQLKYKHVPSAKDASKLIVSVLAGTSLKTLEEKFDFIESSGPKVIRTMPNTSMQVGEGCTVYTGNARVTHQDLEKVHIMLNALGLAQQVPEQMIDAVTGLAGCGPAFVYTIIEALADGGVKQGVPRAMALQFAAQTLLGAAKTVLITGKHPAVLRDEVCSPGGSTIVGVHELEKGNLRATLINAVEKATQRSTELGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNA8</accession>
    <name>A0A6I8UNA8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Coiled-coil protein 142 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4800941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358126.2">
        <property type="nucleotide sequence ID" value="XM_001358089.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNA8"/>
    <dbReference type="KEGG" id="dpo:4800941"/>
    <dbReference type="InParanoid" id="A0A6I8UNA8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026700">
        <property type="entry name" value="CCDC142"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055350">
        <property type="entry name" value="CCDC142_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21436">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21436:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14923">
        <property type="entry name" value="CCDC142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coiled-coil protein 142 C-terminal" evidence="1">
        <location>
            <begin position="341"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358126.2"/>
        </source>
    </evidence>
    <sequence length="771" mass="88710" checksum="ABDFC1DE9F201E6E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPD1</accession>
    <name>A0A6I8UPD1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4801598</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine(1779)/adenosine(1780) in 18S rRNA + 4 S-adenosyl-L-methionine = N(6)-dimethyladenosine(1779)/N(6)-dimethyladenosine(1780) in 18S rRNA + 4 S-adenosyl-L-homocysteine + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10234"/>
            <dbReference type="Rhea" id="RHEA-COMP:10236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.183"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10 11">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001358667.2">
        <property type="nucleotide sequence ID" value="XM_001358630.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPD1"/>
    <dbReference type="FunCoup" id="A0A6I8UPD1">
        <property type="interactions" value="1047"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801598"/>
    <dbReference type="InParanoid" id="A0A6I8UPD1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052909">
        <property type="term" value="F:18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.480:FF:000002">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000007">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 10 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="12">
        <location>
            <begin position="37"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001358667.2"/>
        </source>
    </evidence>
    <sequence length="306" mass="35132" checksum="F2176A591876E43D" modified="2020-08-12" version="1">MPKVKTEKKSRVHNEVQKQGIVFNKDFGQHILKNPLVITTMLEKAALRPTDVVLEIGPGTGNMTVRMLEKAKKVIACEIDTRLAAELQKRVQATPMQPKLQILIGDFLKAELPFFDLCIANVPYQISSPLIFKLLLHRPIFRCAVLMFQREFAQRLVAKPGDKLYCRLSINTQLLARVDMLMKVGKNNFRPPPKVESSVVRLEPKNPPPPVNFTEWDGLTRIAFLRKNKTLAATFKVSSVIEMLEKNYKVFRSLRNEPLEEDFNMQEKVIGILEELEMATLRARSMDIDDFMRLLRAFNSKGIHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPQ6</accession>
    <name>A0A6I8UPQ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein Osi16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Osi16</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358911.3">
        <property type="nucleotide sequence ID" value="XM_001358874.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPQ6"/>
    <dbReference type="FunCoup" id="A0A6I8UPQ6">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801889"/>
    <dbReference type="InParanoid" id="A0A6I8UPQ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012464">
        <property type="entry name" value="DUF1676"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879">
        <property type="entry name" value="FI03362P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879:SF9">
        <property type="entry name" value="OSIRIS 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07898">
        <property type="entry name" value="DUF1676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026338500" evidence="2">
        <location>
            <begin position="26"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358911.3"/>
        </source>
    </evidence>
    <sequence length="277" mass="29712" checksum="063BA2A192C8B300" modified="2020-08-12" version="1">MLLIKTLEYLFYLALFAFMCKYAQAASVQATEASTIATEPLRKPQKSETPLNECEASSFSWMCLKIELVKIMERLAEQQELSVLPGVSVVKDESATEMKTSELMAEVARSYPSDPNTRLNGYIVAKMENLLKTRFLRFRLLDDKALVEGRKHKFGKKGGLEALVAAGVMMKGMLMAMGLGAIALMAGKALMAALMALTLSGVLGLKSLAGGGKSTTYEIVAKPIYTSSHSHSVTHEDGHGHTPHFAAGGGGGGGTASGFGYGGYARSMKPDSQMQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQI3</accession>
    <name>A0A6I8UQI3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mucin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4802400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359325.4">
        <property type="nucleotide sequence ID" value="XM_001359288.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQI3"/>
    <dbReference type="FunCoup" id="A0A6I8UQI3">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="GeneID" id="4802400"/>
    <dbReference type="KEGG" id="dpo:4802400"/>
    <dbReference type="InParanoid" id="A0A6I8UQI3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079947">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026222697" evidence="2">
        <location>
            <begin position="24"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359325.4"/>
        </source>
    </evidence>
    <sequence length="463" mass="51732" checksum="ECA4A39355F79483" modified="2020-08-12" version="1">MLTTSLRPKLWCWMFFLAIHACAQNYATGYQKSDNTGYCPPGYQNVNGYCLSSLNCPAGYYLGTDEKCYQQNALVCPTGYYLGTDGQCYPQNALVCPTGYYLNTDGKCYLKDPLPCPFESTTVIPPVTTQPTTTTTTEEPSTTTTEEPTTTTTEEPSTTTTEEPTTTTTEPPPPPFPPTVERPVELARCPPGSIFFEEQCRRIVCTEGEYYAGRCLLPACPAGTVWHGGSCQEPGYITTILEIDNVIRNQHEYTVATENINRVEYSTHPPYDEAYDKSYDATTRHEESNDIITTTTKRPWLYPTGQPTTMALPPTSEVFPGRNPPPGCCLVKSPRICINYAPNWVCSSRERKLCDPRVCTNSVIYLKPPQIVESENRRRLVMPPNPPLQACSTPECKESEFLDCSGCKHDQRDKCSPGCYSYYCPNGICSFMNSQDYCGIYPSGFGCNADDGCIWDWCHKKCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URB9</accession>
    <name>A0A6I8URB9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-galactosidase</fullName>
            <ecNumber evidence="7">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Ect3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001359480.4">
        <property type="nucleotide sequence ID" value="XM_001359443.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URB9"/>
    <dbReference type="FunCoup" id="A0A6I8URB9">
        <property type="interactions" value="686"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802587"/>
    <dbReference type="InParanoid" id="A0A6I8URB9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000021">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000017">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026283">
        <property type="entry name" value="B-gal_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048912">
        <property type="entry name" value="BetaGal1-like_ABD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21317">
        <property type="entry name" value="BetaGal_ABD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006336">
        <property type="entry name" value="B-gal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase 35 catalytic" evidence="9">
        <location>
            <begin position="49"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase 1-like first all-beta" evidence="10">
        <location>
            <begin position="416"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase galactose-binding" evidence="11">
        <location>
            <begin position="563"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359480.4"/>
        </source>
    </evidence>
    <sequence length="654" mass="73611" checksum="F028333B9D5D0B1B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URU1</accession>
    <name>A0A6I8URU1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4803117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359913.4">
        <property type="nucleotide sequence ID" value="XM_001359876.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URU1"/>
    <dbReference type="KEGG" id="dpo:4803117"/>
    <dbReference type="InParanoid" id="A0A6I8URU1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001359913.4"/>
        </source>
    </evidence>
    <sequence length="291" mass="33775" checksum="84689BCF0787E29F" modified="2020-08-12" version="1">MALVQTCAIDNNSDAWLIWMSREASSKGEKLSLDPPRIVAFHFFDNRHRDDVTTKKWAKLMAKLAHEYAGRIMFGMRDMKSIGNWHPNLRPNDFGGYRPGLPPLIYGEDKKHNVYEMNRLVNYRNMKDFCEKLLQDQLFQAVVVAPVAELDSPPQNYFDLEDHKDRDYFVMLYDPACYHWPMQLKMLRKLSLLLANETISVMIINKSHNYLGVVFNKFSKAVNCHGATIFSTSRLDGWDFKMRPRQDSTREYLRYIAQSRQPELLGYDANGEPKAPEAALAYIECLFAESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US13</accession>
    <name>A0A6I8US13_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase ATM</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">tefu</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001359890.5">
        <property type="nucleotide sequence ID" value="XM_001359853.5"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8US13">
        <property type="interactions" value="1766"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803091"/>
    <dbReference type="InParanoid" id="A0A6I8US13"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000023">
        <property type="entry name" value="Serine/threonine-protein kinase ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="15">
        <location>
            <begin position="1720"/>
            <end position="2318"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="14">
        <location>
            <begin position="2420"/>
            <end position="2738"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="16">
        <location>
            <begin position="2739"/>
            <end position="2771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001359890.5"/>
        </source>
    </evidence>
    <sequence length="2771" mass="317645" checksum="A209A786E90D83EB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USC5</accession>
    <name>A0A6I8USC5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine protease K12H4.7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4803255</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360027.1">
        <property type="nucleotide sequence ID" value="XM_001359990.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USC5"/>
    <dbReference type="FunCoup" id="A0A6I8USC5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="4803255"/>
    <dbReference type="KEGG" id="dpo:4803255"/>
    <dbReference type="InParanoid" id="A0A6I8USC5"/>
    <dbReference type="OMA" id="CERFDVY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077661">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF5">
        <property type="entry name" value="RE36938P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026012160" evidence="6">
        <location>
            <begin position="20"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001360027.1"/>
        </source>
    </evidence>
    <sequence length="473" mass="53219" checksum="D72D1F8857D4CD0F" modified="2020-08-12" version="1">MKLWLAITLVALALVETKGSIFENTFKRLHEEPPLPSNQNRADVVQTLWIEQKLDHFDEDEKRTWQMRYMLNDALYQSGGPLFIYLGGEWEISAGRITGGHIYDMAKEHNALLAYTEHRYYGESKPLPDLSNENIQYLNVRQALEDLAVFIRTLKATHEGLSESKVIIVGGSYSATMVTWFKKVHPDLVAGGWASSAPLFAKVNFVEYKEITGQSIALMGGSACYNRIESGIAEMETMFATKRGAEVKALLKLCERFDVYSDLDVWTLFSEISDLFAGVVQTHNAGQIEAVCQKIMSGSNDLIGVASYLLDVFSESGGKCNELSYDAILSQLLDTSYTGNIMRQWIFQTCNEYGWYQTSDSKAQPFGTKFPVALYTTMCGDIYGSQYSNEFIDSRVAATNDYFGGWTPGVENVYLTHGHLDPWRAMGIQDEAQATVIPEYAHCKDFNSISDTDTAEMRASKERIAELVREWIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USX1</accession>
    <name>A0A6I8USX1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Gr59d</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360400.3">
        <property type="nucleotide sequence ID" value="XM_001360363.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USX1"/>
    <dbReference type="FunCoup" id="A0A6I8USX1">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803728"/>
    <dbReference type="InParanoid" id="A0A6I8USX1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001360400.3"/>
        </source>
    </evidence>
    <sequence length="393" mass="45654" checksum="C9D5872EAC139DF3" modified="2020-08-12" version="1">MPDLVKWCVRISYLYGRVTGTLNFEIDLKTGRTRVTKKATIYSALAHLCLITMLVHHLWRVKPTSDLLANANALHENVFMVVAWMRVSCALAALAGRWYHRRRYMRLISSFRRLYLKNTEVMQHCRRGFVSKCFITTVAESMQFLMALLVVWDRLTFSLLIGIWSVMTVTAVMNVIITQYYFALGNARGHYKLLNRDLREVLAEARSLGPKRKRQNGVFITKCCFLADRVDEIAQTQSELQALIERMSRIYELQVLCLFCTYYLTSVGNAYLLFSIYKYNNITQGWSKLSLLAGATFLVFYYADCLINSYNVFYLLEAHQEMHKLLEQRNLFPWGLDERLESAFDSLELSLARNPLQLHCFGLFKLDRSSAFDVGNSLLINSVLLIQYDMQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTE2</accession>
    <name>A0A6I8UTE2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Polr1A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="RefSeq" id="XP_001360603.2">
        <property type="nucleotide sequence ID" value="XM_001360566.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UTE2">
        <property type="interactions" value="1643"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803965"/>
    <dbReference type="InParanoid" id="A0A6I8UTE2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000012">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000014">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 19" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="293"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1337"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1362"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1389"/>
            <end position="1428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001360603.2"/>
        </source>
    </evidence>
    <sequence length="1647" mass="185445" checksum="2F730499B3BF5194" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTH5</accession>
    <name>A0A6I8UTH5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Regulatory protein zeste</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">prod</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in transvection phenomena (= synapsis-dependent gene expression), where the synaptic pairing of chromosomes carrying genes with which zeste interacts influences the expression of these genes. Zeste binds to DNA and stimulates transcription from a nearby promoter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Self-associates forming complexes of several hundred monomers.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360684.3">
        <property type="nucleotide sequence ID" value="XM_001360647.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTH5"/>
    <dbReference type="FunCoup" id="A0A6I8UTH5">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="GeneID" id="4804063"/>
    <dbReference type="KEGG" id="dpo:4804063"/>
    <dbReference type="InParanoid" id="A0A6I8UTH5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075037">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="7">
        <location>
            <begin position="8"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360684.3"/>
        </source>
    </evidence>
    <sequence length="353" mass="39843" checksum="9CCEF2A00A2EA65E" modified="2020-08-12" version="1">MLGKMDRRKKRTSSEQYQMYIDMMEADSIFATGRVPRDYDLNYLTKKWKELSDRLNKCSSGPTLTAEEWRKRLNDWKNTTRCKYRRSLMATEKDISMTTLENRALDLFGKVPSTTGETLLNLKSEKDDGDDEIEEIGQRTSAAFQKELQAAVEEAINDEVDDVDDMLEEHVEQEDIIDDNLADNSGAMESSSAATTAVNTGGGTYRTIVVDNTSFEHVEDPHPVEFVTARRTPASSNSSVHAVINSGPVVETKLINGELPVKRLRTQTRPDQIIYHVKNAPRCISNIQDVPPLLSTKVERDTTTLSAALSSQDGQQIAHQLKRLADINYETLQFEIARFKFNNPGFQYNPPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTL8</accession>
    <name>A0A6I8UTL8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Phosphoglucomutase-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Pgm2b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360584.3">
        <property type="nucleotide sequence ID" value="XM_001360547.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTL8"/>
    <dbReference type="FunCoup" id="A0A6I8UTL8">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803947"/>
    <dbReference type="InParanoid" id="A0A6I8UTL8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05799">
        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="66"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="236"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="353"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001360584.3"/>
        </source>
    </evidence>
    <sequence length="629" mass="71411" checksum="2CD320C51423B219" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTP4</accession>
    <name>A0A6I8UTP4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 186</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4804164</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM186 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360768.4">
        <property type="nucleotide sequence ID" value="XM_001360731.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTP4"/>
    <dbReference type="FunCoup" id="A0A6I8UTP4">
        <property type="interactions" value="2039"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804164"/>
    <dbReference type="InParanoid" id="A0A6I8UTP4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026571">
        <property type="entry name" value="Tmem186"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045325">
        <property type="entry name" value="TMEM70/TMEM186/TMEM223"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13603">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13603:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06979">
        <property type="entry name" value="TMEM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001360768.4"/>
        </source>
    </evidence>
    <sequence length="235" mass="26152" checksum="7C1DC590F5007DEC" modified="2020-08-12" version="1">MTEWAALANTCASADGVSSITAQLIDDIFKTNAMLQQLCRSHFTRVRWPSSVPAVIQQLHQRRLGTEPKKNPILNETGFSEWRTIYSLPSIRLVAAFNRLKIYQAVVTAAGAPIAFALEQSGHATDALGIYAAIGISGLITLTLASFAATNLVGFIYINEQQDMLKLAYVDFWGRRVETLVETDDLLPSWERGTMSQLRFVLPIALRSDAKRRYKLLNRFGNIADPQLFEGLFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUD8</accession>
    <name>A0A6I8UUD8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein angel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">angel</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361080.2">
        <property type="nucleotide sequence ID" value="XM_001361043.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUD8"/>
    <dbReference type="FunCoup" id="A0A6I8UUD8">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804532"/>
    <dbReference type="InParanoid" id="A0A6I8UUD8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF34">
        <property type="entry name" value="PROTEIN ANGEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="73"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361080.2"/>
        </source>
    </evidence>
    <sequence length="359" mass="41110" checksum="E8A24CD458A62480" modified="2020-08-12" version="1">MYKFLLRSLPGQASFIRTLGISTAKHRSSRRNESSDQIDDAMDRYDRNRQWRRTKDGHGHGHVSKDSSFKVVSYNILAQDLLVEHFYLYGELRTDCLTWRRRLQNIQREILTLDPDILCLQEMQYDHIFDLMQGLRMGNGKKLEYVYKKKTGERTDGCAIIYDACKFQLLDQRPIEFYDQNVKLLNRENVALFAKLGMKGQTEKEFIVATTHLLYNPKRDDVRCAQVTRLLEELETFSCDPKSGQVTPIVLTGDFNSVPNSPPFEIITADSPPAKLLNFKAVEMGPPGPKGPGIASTRQDNWITVDYILRSLSDKSQHQLQVTSIYGLPTVNVCYRVGPIPNHYLGSDHYALGVVFNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV33</accession>
    <name>A0A6I8UV33_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tumor necrosis factor alpha-induced protein 8-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sigmar</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361077.3">
        <property type="nucleotide sequence ID" value="XM_001361040.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV33"/>
    <dbReference type="FunCoup" id="A0A6I8UV33">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8UV33"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UV33">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.160:FF:000001">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 8-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.160">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008477">
        <property type="entry name" value="TNFAIP8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038355">
        <property type="entry name" value="TNFAIP8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12757:SF1">
        <property type="entry name" value="PROTEIN SALIVARY GLANDS MARRED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12757">
        <property type="entry name" value="TUMOR NECROSIS FACTOR INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05527">
        <property type="entry name" value="TNFAIP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026304458" evidence="1">
        <location>
            <begin position="25"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361077.3"/>
        </source>
    </evidence>
    <sequence length="210" mass="23933" checksum="7499D4BF456ACE9A" modified="2020-08-12" version="1">MSVAGLRQCLLLWSVDYWNLEVMADNVFKSQDIGLRAQKKILSRMATKNIAKTFIDGTTASLLDNLYRLCKMHTGNKAKAEKLIKNIIKIVIKIGVLHRNNQFSDEELQRAENFKRKFQNTQLSIISFYEVDYTFDLAYLQKSIAESQVALKSIVQPHLTDKSLGRIDEVFDFFGDAVLLETAFKPDSPYREVMGKIVVDINSAMETGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV74</accession>
    <name>A0A6I8UV74_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="10">AK6</shortName>
            <ecNumber evidence="10">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="10">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ak6</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer and homodimer. Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001361417.1">
        <property type="nucleotide sequence ID" value="XM_001361380.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV74"/>
    <dbReference type="FunCoup" id="A0A6I8UV74">
        <property type="interactions" value="2034"/>
    </dbReference>
    <dbReference type="GeneID" id="4804935"/>
    <dbReference type="KEGG" id="dpo:4804935"/>
    <dbReference type="InParanoid" id="A0A6I8UV74"/>
    <dbReference type="OMA" id="QCEIFGT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081330">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000372">
        <property type="entry name" value="Adenylate kinase isoenzyme 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="10">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="10">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361417.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="20457" checksum="D8B6FF68E115B74A" modified="2020-08-12" version="1">MSDQNADAKPNILITGTPGAGKSYLCERLAEQLKFDWLDCSKIAKENDYIEEHDEEYDCPILDEERLMDHLEPLMQKGGNIVEYHGCDFFPERWFQAVFVVTCPNKTLYDRLKERNYNEKKLSSNIECEIFGTILEEARDSYKADIVHELCGESAADADKSLKTVKQWYRMWKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWL2</accession>
    <name>A0A6I8UWL2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Bardet-Biedl syndrome 4 protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">BBS4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the BBS4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001362016.1">
        <property type="nucleotide sequence ID" value="XM_001361979.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWL2"/>
    <dbReference type="FunCoup" id="A0A6I8UWL2">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="GeneID" id="4805649"/>
    <dbReference type="KEGG" id="dpo:4805649"/>
    <dbReference type="InParanoid" id="A0A6I8UWL2"/>
    <dbReference type="OMA" id="YCEVAWH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072191">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000829">
        <property type="entry name" value="Bardet-Biedl syndrome 4 protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44186">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44186:SF1">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001362016.1"/>
        </source>
    </evidence>
    <sequence length="483" mass="55072" checksum="9170615EA4BB8185" modified="2020-08-12" version="1">MYEAGAERINCNGRIIELPSLEVVRPAPKMPSDANIDWLLHIYFTRREFSRCRRLIGRELNRHLNAEYLYFVLGLIDREEGNHIEALRHLQRSVELNPKNIETYKEIGRTLYVMGRFSQALDVFREAEQSSPRKDHEIYHYLGELLYRSATTQSQLEMAKKQQDEARAYFELAVQTGKKMESHVRLAELYRKDKQYQQAIDVLENCLHLTPENAEVLIEISVLYLKINETQKAYDRLAEVVIERKCSPKGLLAFGAILQSRNDVDGALSKYSQIANAEPEIAELWNNIGLCFFKKQKFIAAVSSLRKSVWLSPLNYNALYNLSLIYIASEQYASAFHTLAAAINLRKDNAECYMLLGLCLRKLDDVDNAFVALERSSSMASGQQGAGRNPLVFLNFALFCYETGRLALATEQYNRFMSQAQDLMLPTEYKFQATKLKSLLRISNQGNGMPLEAAESDEAALATAPELRPDELPLEVNAVVSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWP1</accession>
    <name>A0A6I8UWP1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ACOX1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:38960"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361780.1">
        <property type="nucleotide sequence ID" value="XM_001361743.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWP1"/>
    <dbReference type="FunCoup" id="A0A6I8UWP1">
        <property type="interactions" value="1731"/>
    </dbReference>
    <dbReference type="GeneID" id="4805369"/>
    <dbReference type="KEGG" id="dpo:4805369"/>
    <dbReference type="InParanoid" id="A0A6I8UWP1"/>
    <dbReference type="OMA" id="WNMYNVL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078592">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01150">
        <property type="entry name" value="AXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000033">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000013">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000003">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034171">
        <property type="entry name" value="ACO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF250">
        <property type="entry name" value="PEROXISOMAL ACYL-COENZYME A OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13 15" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="18">
        <location>
            <begin position="20"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="17">
        <location>
            <begin position="149"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="19">
        <location>
            <begin position="288"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="16">
        <location>
            <begin position="484"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001361780.1"/>
        </source>
    </evidence>
    <sequence length="669" mass="74626" checksum="00B30B4F278CC875" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWW5</accession>
    <name>A0A6I8UWW5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein tiptop</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">tio</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the teashirt C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132274.2">
        <property type="nucleotide sequence ID" value="XM_002132238.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWW5"/>
    <dbReference type="FunCoup" id="A0A6I8UWW5">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903395"/>
    <dbReference type="InParanoid" id="A0A6I8UWW5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027008">
        <property type="entry name" value="Teashirt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
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    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487:SF7">
        <property type="entry name" value="PROTEIN TEASHIRT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487">
        <property type="entry name" value="TEASHIRT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="500"/>
            <end position="529"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="132"/>
            <end position="228"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="246"/>
            <end position="295"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="347"/>
            <end position="387"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="462"/>
            <end position="492"/>
        </location>
    </feature>
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            <begin position="520"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="634"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="735"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="899"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="980"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
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        <location>
            <begin position="561"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="740"/>
            <end position="762"/>
        </location>
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        <location>
            <begin position="767"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="785"/>
            <end position="809"/>
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        <location>
            <begin position="822"/>
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            <begin position="904"/>
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            <begin position="1005"/>
            <end position="1025"/>
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            <dbReference type="ARBA" id="ARBA00007158"/>
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            <dbReference type="ARBA" id="ARBA00022473"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_002132274.2"/>
        </source>
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    <sequence length="1057" mass="110497" checksum="614DBFDE5BCB8BB8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX46</accession>
    <name>A0A6I8UX46_DROPS</name>
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        <recommendedName>
            <fullName evidence="9">PLAT domain-containing protein</fullName>
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        <name evidence="11" type="primary">LOC6903498</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
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        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <source>
            <dbReference type="RefSeq" id="XP_002132377.3"/>
        </source>
    </evidence>
    <sequence length="1475" mass="173099" checksum="0B2729A77700F347" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXA6</accession>
    <name>A0A6I8UXA6_DROPS</name>
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        <recommendedName>
            <fullName evidence="4">Forkhead box protein G1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="primary">LOC6902840</name>
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    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_002132576.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="13019" checksum="88370595E7B0AA80" modified="2020-08-12" version="1">MPYYEEERRHHHHHHHGGRPIVEVDIIPPRIPRPVIEIDVGGRRPPPPRVEVITPAAVYQPPYQPQYQQQRPIVEVDVVQPGRPFIEFNIGGRRPPPREEIIVVQQPPPRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXP6</accession>
    <name>A0A6I8UXP6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein zeste isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002132766.3">
        <property type="nucleotide sequence ID" value="XM_002132730.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXP6"/>
    <dbReference type="FunCoup" id="A0A6I8UXP6">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903042"/>
    <dbReference type="InParanoid" id="A0A6I8UXP6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXP6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071339">
        <property type="term" value="C:MLL1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016589">
        <property type="term" value="C:NURF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397:SF2">
        <property type="entry name" value="CHROMATIN COMPLEXES SUBUNIT BAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397">
        <property type="entry name" value="CHROMATIN COMPLEXES SUBUNIT BAP18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132766.3"/>
        </source>
    </evidence>
    <sequence length="340" mass="36592" checksum="E7B4989F37CEAC98" modified="2020-08-12" version="1">MNSATKVGEIFTAAGQAFSRLGDLTMQLHPNAESPSGKWTDEEIDMLHSSIMRFSDDLSKISLSIKNRTVSQIRQALKKKAFEDAGIPAKQVPVQQVHHVIQTVPQLPVIQQQQLTTAPQLQQSHQHQPQVFKTQTVQQLPLQQHQHQHQPQNSNMAASIQHVQLVTQPKQILLQQHQQQLPQTTTTTVTIKQFQQMQQQKAAALAAAAAAAAAANTPTITENIIIQQPTSTTAAAQQPQSHVHPVHSVATSASTQIVVPSSVTTVLSSSTGAVGPVAIADDVVSTTSTPSTSASTGLKCGPDVSMTLNRINVQENEVDVEECLPAEVVKLDFVSEEVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYP1</accession>
    <name>A0A6I8UYP1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Probable cytochrome P450 311a1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Cyp311a1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133517.2">
        <property type="nucleotide sequence ID" value="XM_002133481.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYP1"/>
    <dbReference type="FunCoup" id="A0A6I8UYP1">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901983"/>
    <dbReference type="InParanoid" id="A0A6I8UYP1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF201">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 4, SUBFAMILY B, POLYPEPTIDE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026239222" evidence="15">
        <location>
            <begin position="22"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002133517.2"/>
        </source>
    </evidence>
    <sequence length="483" mass="55026" checksum="1F307F7CDBB34657" modified="2020-08-12" version="1">MPCWVLLLVGLGLLLLLQKWALLRLGSSLPGPWAFPLLGNAQMVGKLRPEFIFLVFTELRDRFGATYRLWLGPQLWVFLHTAEETREALHDPTLRKAKTFLQLEPLIGNGLLISHGSHWTQQRRLLTPAFRPTLLRSFAPSVAKHADRLIQRLEETRGSFLEVTDPLFACLLDAIVDTSMGEQLETQSVAHSPIIKAFHRSSQLLFKRMINPLLASDWVFKQTRLWRELSEQLQVIHTLMDSLIDRRSAQLQQQGNEPRKALNLLDTLLLANIDGEPLSRKEIRDEINTFVFAGVDTTTAAMSFVLYALAMNPEAQDRLQEELRQLPPGISIDLDTLNELPYLDALLKEVLRLYTIVPTTGRQTTRSTVIGGRTYCAGVTLWINMYGLAHDASYFPDPYVFKPERWMQETELQAEVPFSYIPFSGGPHVCIGRRYALLLMKLLTARIARAFQLELRPGQPELTLQAQMVLKTREGIHLAFTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYP7</accession>
    <name>A0A6I8UYP7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ADP-ribosylation factor-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6901990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133524.3">
        <property type="nucleotide sequence ID" value="XM_002133488.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYP7"/>
    <dbReference type="KEGG" id="dpo:6901990"/>
    <dbReference type="InParanoid" id="A0A6I8UYP7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002133524.3"/>
        </source>
    </evidence>
    <sequence length="283" mass="32031" checksum="3674DE8B83FD870C" modified="2020-08-12" version="1">MGVLCAKLRRYFCSSCCCFRALPPPEEFQVLLLGPAGSGKTELGHRVSGRERGGDDLDPTNGVRCYRLESAALQREDIAVDLQLTEVGGNAEMQRLWKHYYAASHALIYCFDLGSGMEELEDTFALLRKCLQGTALDGKPVLLVAKRHRDGVQLYDVEHSFGLDQLARTCGCPLHICHMDDARDLGHGIVWLCRQLVARRPMLEQRVRYDVNMQVWHRRKKSLLSRSKMAQVHRGRFRRPNRKLWPTVNSVNHTTVRPSTAPPTIFLVRSRQEAAPNPEQAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZF7</accession>
    <name>A0A6I8UZF7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ctenidin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134080.3">
        <property type="nucleotide sequence ID" value="XM_002134044.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZF7"/>
    <dbReference type="KEGG" id="dpo:6901392"/>
    <dbReference type="InParanoid" id="A0A6I8UZF7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026192458" evidence="2">
        <location>
            <begin position="18"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002134080.3"/>
        </source>
    </evidence>
    <sequence length="222" mass="17827" checksum="3D1C7E47FE0C506B" modified="2020-08-12" version="1">MKAFVCLLLIGAASVTAKPKPGGSSGGGGGWSSSGGGGGGGGWSSSGGGGGGHGGGGGGDIQIIKVITESGGGGGGGGGGWQSGGGGGGGGDWSSGGGGGGGGGWSSGGGGGGGGGDVKLIKIISLGGGGGGGGGHGGGGGGGGWSSGGGGGGGGWSSGGGGGGGGHGGGGGGGTKVIKIIKLSSGGGGGGGHGGGGGGGGGGWSSGGGGGGGGWQPAGGWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V025</accession>
    <name>A0A6I8V025_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA polymerase II degradation factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134693.3">
        <property type="nucleotide sequence ID" value="XM_002134657.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V025"/>
    <dbReference type="KEGG" id="dpo:6900997"/>
    <dbReference type="InParanoid" id="A0A6I8V025"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V025">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134693.3"/>
        </source>
    </evidence>
    <sequence length="340" mass="36266" checksum="13C897D006F613F7" modified="2020-08-12" version="1">MEAKRGNNASATRHATHASRTSVASRHGQQPAGAGGQGATMSRKHVSEASLTPSQARRDTDNPRKSPLDALSRAGVVRQQDPFQRRSGLQDVDEAFLATNAFARPSRVRHSGLDGWTDAGAAVPAAAPAAMAPIRVTGGQDQAQVVYQQQTQSQQGMPLQAAAGTQDQQQMPPTLQQTPPVAAVPSLQQFNQPAAGQPTRHEPYPTGRAHVHHNQQQQQQQVAYDQQQHQQQIAQQETQAIGQSQSQGQLSPRKYQQQPQTQGFPNQPNVGGGERAAAGGPGSGGQQQQQQQQQQQQHVGGGAAPPPEAEMCTTCPNCQTTIYLVRSAEMGHGDNSLPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V098</accession>
    <name>A0A6I8V098_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Clavesin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134868.3">
        <property type="nucleotide sequence ID" value="XM_002134832.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V098"/>
    <dbReference type="FunCoup" id="A0A6I8V098">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900478"/>
    <dbReference type="InParanoid" id="A0A6I8V098"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V098">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF166">
        <property type="entry name" value="LD40136P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="115"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134868.3"/>
        </source>
    </evidence>
    <sequence length="334" mass="38019" checksum="C0849BC530AB3635" modified="2020-08-12" version="1">MLETEVLQQPKLNGPMASGGDYDYPYVCGLSADMKVVAKEELHEDENIRRQALAQFREWIDKHPHIKKCRTDAVFLLRFLRTKKFSVPSACEMLERYLTIRQLFPQWFQHLDINDPAINEIFENGYLVPLPQRDAIGRQVIFSVAAKFDPYKFTSVQMARVHSLICEALLDDEDSQVAGYVYINDESGMNMGFVSLWSLTDLRSIVKCIQNSTPMRHKETHFVNIPHYANRIIELGVSMLSDKLKKRIIVHKNVDALSKSKIDPAILPKEYGGTVPMADMIAQFKQKLVQRRAAILALDDMRIEITKDAANFAGNDIGDLDAGVVGSFRKLEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1J3</accession>
    <name>A0A6I8V1J3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Peroxin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">Pex1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with PEX6; forming the PEX1-PEX6 AAA ATPase complex, which is composed of a heterohexamer formed by a trimer of PEX1-PEX6 dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134929.3">
        <property type="nucleotide sequence ID" value="XM_002134893.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1J3"/>
    <dbReference type="FunCoup" id="A0A6I8V1J3">
        <property type="interactions" value="2021"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900545"/>
    <dbReference type="InParanoid" id="A0A6I8V1J3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V1J3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19526">
        <property type="entry name" value="RecA-like_PEX1_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000105">
        <property type="entry name" value="PeRoXisome assembly factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015342">
        <property type="entry name" value="PEX1-N_C-lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF12">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09262">
        <property type="entry name" value="PEX-1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="478"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="755"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="175"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00064205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_002134929.3"/>
        </source>
    </evidence>
    <sequence length="1008" mass="113887" checksum="F835FA43ABAE62B1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2J5</accession>
    <name>A0A6I8V2J5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137293.3">
        <property type="nucleotide sequence ID" value="XM_002137257.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2J5"/>
    <dbReference type="FunCoup" id="A0A6I8V2J5">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897109"/>
    <dbReference type="InParanoid" id="A0A6I8V2J5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026323723" evidence="2">
        <location>
            <begin position="25"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137293.3"/>
        </source>
    </evidence>
    <sequence length="237" mass="25286" checksum="3003CB555BF1F02E" modified="2020-08-12" version="1">MGPGQLSLGCLLLIVSFTVHTVSGAICKNTQILTENKGCVDREGYLERVLLRTWVAEDLDDAKKDAGIGKHVRCNADEILTATGCSQQTEAPRQAESFRVPASHSKLQGDQVVKSDSLVGGGGGGVGGGGGGGGGGGGGASHVSAPAPQPALAPRTGQIQGRTQMGRPRVIGHNRPRKFVILPGRQLESGRKCRPYEVHAKDNRCVRKSPRKPTPIYRHKDHRYGLQLRHRHRSKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2Z3</accession>
    <name>A0A6I8V2Z3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Circadian clock-controlled protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6897357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137510.3">
        <property type="nucleotide sequence ID" value="XM_002137474.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2Z3"/>
    <dbReference type="KEGG" id="dpo:6897357"/>
    <dbReference type="InParanoid" id="A0A6I8V2Z3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20993">
        <property type="entry name" value="GH07914P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20993:SF0">
        <property type="entry name" value="GH07914P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002137510.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="33783" checksum="D729DF618882643D" modified="2020-08-12" version="1">MQSIIRFGISQRKYNQEIKNRAIKDTRRDHKPVTVHLPTESAIKMASQLFLVALLGIALVSATPVPQQAASANFSSALTRYIQAFKSIMPCGYAPDIPVLAPIVNPFYAFDFSSNAYSLKGNVSNIRIEGLNNFQILSASDYGTTGEAAYDVIFPKIQVLGSTDVEGSINFAGYDVPIRQRDLINEAFVDLRFVGSYSFANSLTNSSGLRIVDFKLAIYLADIQQDNWNTLWNISSNNLANSWGKKLLYFALQEIQPKITDSLYQNLLVPKINEMLENVSMEELVNYFQSQAQLWENAHCQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V338</accession>
    <name>A0A6I8V338_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Luciferin 4-monooxygenase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6897632</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137775.2">
        <property type="nucleotide sequence ID" value="XM_002137739.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V338"/>
    <dbReference type="KEGG" id="dpo:6897632"/>
    <dbReference type="InParanoid" id="A0A6I8V338"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000025">
        <property type="entry name" value="luciferin 4-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
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    </dbReference>
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        <property type="entry name" value="AMP_BINDING"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="41"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="449"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002137775.2"/>
        </source>
    </evidence>
    <sequence length="550" mass="61838" checksum="B0F650D304C7C757" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4A2</accession>
    <name>A0A6I8V4A2_DROPS</name>
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        <submittedName>
            <fullName evidence="2">Protein NCBP2AS2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898433</name>
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    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138475.1">
        <property type="nucleotide sequence ID" value="XM_002138439.3"/>
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    </dbReference>
    <dbReference type="GeneID" id="6898433"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246177">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042407">
        <property type="entry name" value="NCBP2-AS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41161">
        <property type="entry name" value="PROTEIN NCBP2AS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41161:SF1">
        <property type="entry name" value="PROTEIN NCBP2AS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002138475.1"/>
        </source>
    </evidence>
    <sequence length="84" mass="10021" checksum="9F7C48A6E9AFB559" modified="2020-08-12" version="1">MVLRLLMRYLANNEQLIQRMADSYPMRRAAQLVVSLMYRTKSLAREQGLHEMTPERFKSFVSMFKNNVKQELEGVKKDLKNRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4B3</accession>
    <name>A0A6I8V4B3_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Calmodulin-related protein 97A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6897693</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137819.1">
        <property type="nucleotide sequence ID" value="XM_002137783.3"/>
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        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="GeneID" id="6897693"/>
    <dbReference type="KEGG" id="dpo:6897693"/>
    <dbReference type="InParanoid" id="A0A6I8V4B3"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247696">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="80"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="116"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002137819.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="17136" checksum="8361207DAB18C80A" modified="2020-08-12" version="1">MTELTEEQIAEFKEAFALFDKDGTGSITTRELGILMRSLGQNPTEAELQDLVNEVDIDGNGEIDFNEFCQMMNKQKRESDTEEEMREAFQIFDRDHDGFISPAELRFAMINLGEKVTEEEIDDMVREADFDGDGLINYEEFVWMINQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4G0</accession>
    <name>A0A6I8V4G0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein RhoGAP54D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RhoGAP54D</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138617.2">
        <property type="nucleotide sequence ID" value="XM_002138581.3"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8V4G0">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898612"/>
    <dbReference type="InParanoid" id="A0A6I8V4G0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AGAP001687-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF7">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="94"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="575"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="668"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="803"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="847"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="689"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="815"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002138617.2"/>
        </source>
    </evidence>
    <sequence length="999" mass="110217" checksum="F659BC56AF300FB5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5E7</accession>
    <name>A0A6I8V5E7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extensin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC13036484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736177.2">
        <property type="nucleotide sequence ID" value="XM_003736129.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5E7"/>
    <dbReference type="KEGG" id="dpo:13036484"/>
    <dbReference type="InParanoid" id="A0A6I8V5E7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026025026" evidence="1">
        <location>
            <begin position="23"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_003736177.2"/>
        </source>
    </evidence>
    <sequence length="233" mass="23490" checksum="5E73B01B030EE6E6" modified="2020-08-12" version="1">MLNISVALVGALICLGAAPALGSFFHGHAPAPAAVGVPVGVPFPVHVPVPVPSPVPVAVPAPVAVPAPVAVSDTVTVPAAYSYASVPSFYHAAPPPPKAHFNYAAAYAAPAPAIKYSLGTPVTTTTTTYTGYAAPPPPPPPQYAALPPLQYAAPPPLQYAAPHYAAPPYAAPARSQFLSRFAPPPGYQLAAAAPAFHGRYKLHFGAPPPSPAAVYGAPPPPAPSLSYGANQGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6M9</accession>
    <name>A0A6I8V6M9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ergosterol biosynthetic protein 28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4800902</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736349.2">
        <property type="nucleotide sequence ID" value="XM_003736301.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6M9"/>
    <dbReference type="FunCoup" id="A0A6I8V6M9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="GeneID" id="4800902"/>
    <dbReference type="KEGG" id="dpo:4800902"/>
    <dbReference type="InParanoid" id="A0A6I8V6M9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074456">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005352">
        <property type="entry name" value="Erg28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451:SF19">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03694">
        <property type="entry name" value="Erg28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_003736349.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19670" checksum="28E3D6DAE37D0BB0" modified="2020-08-12" version="1">MAAMQSSGRPRERLLYAFRGWIAFVAFMDLGTAFRSYIERRSFLGDHSDTQFIEGDYTISRIIGMYCLLKAIALVHCTLYIHYRPVVSMGGCSLALTMVFYATEALYFRSSTLNFYVIFPCVLNSITLLGLIYIPRRLRLWEPNMDLDDENSQLLKQMTGYKRRRTKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V8Y0</accession>
    <name>A0A6I8V8Y0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4817089</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_015036226.2">
        <property type="nucleotide sequence ID" value="XM_015180740.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V8Y0"/>
    <dbReference type="FunCoup" id="A0A6I8V8Y0">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817089"/>
    <dbReference type="InParanoid" id="A0A6I8V8Y0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF30">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015036226.2"/>
        </source>
    </evidence>
    <sequence length="447" mass="50571" checksum="2F57FD4CF7832BF1" modified="2020-08-12" version="1">MIIRLRYGRRLQTYLKIGACVLVGVCYFAFLFRESLNAYEHRERATAAELEADAAEPSKQQLKLKRQQMQRQRLLAQLQAAQAGTTGTNATGSSATAVAAPLPPASTLNPVYEYSEELHSSTERELQRRREHLAAVCERYKLQEKYPPNPWEFFVSPGHNNLVWCNVFKAASSTWMYYFNVLAGYDVKYLQRTETQPLELARKRFPRPELAELMELLPSALSFLFVRDPFERILSAYRNKLEGNRNTFYKALGTKIVHRFRNHSQGGPWPRCGPTFEEFVRFLIAEHGAGKRFDEHWAPIYSFCTPCSVNFTIIGKTETFQRDSEFIIRQAGLETLLLGLGKLPQRKQRRIGNQARSGIKSEAVVERYFADIDRSTLDQLLKIYRIDFELFDYDYRKYYDMVHPWSLEQSTAGTAAAAAAAAPATTTAAPAVAAAGASGSTQGSAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAJ2</accession>
    <name>A0A6I8VAJ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Neurofilament medium polypeptide isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">w-cup</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036672.2">
        <property type="nucleotide sequence ID" value="XM_015181186.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAJ2"/>
    <dbReference type="InParanoid" id="A0A6I8VAJ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VAJ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="174"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036672.2"/>
        </source>
    </evidence>
    <sequence length="363" mass="40858" checksum="0B2F5A450702F2DD" modified="2020-08-12" version="1">MEARALSEGQVGIEMSKEFIQFGQGSPAGLEEFGPLTEKSPRAMQNQEQEKQIMVEAVEAEEDQSSVDGKQQESQDCAENDLTNAEPGLEIPVEKSSENYSETEYECSSLIEIEIASEESKEETLTAEERAKMKEKEAKEQAKKESAMAKEERKLKEKQLKEAKRREKLNVLLEEARSLGKSPKPEVPATEASPEAKPQSPPGDVAGAKKKTKKAKKAAKWRVDSAKMEEDIQQFLEAIKALEKKQREADRQEAEEAAAIVIENEHMRGWNSVLKELGQTSTFSAATASLSAAQLSSAPRLVVRAILHRVAKMEYKNQLHVLEETYQYRSSLLKRLKLDMRSNYKRELQELSAAYKKSKPQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAM7</accession>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC26531961</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_015181235.2"/>
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    <dbReference type="KEGG" id="dpo:26531961"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026046759" evidence="2">
        <location>
            <begin position="20"/>
            <end position="107"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036721.2"/>
        </source>
    </evidence>
    <sequence length="107" mass="11552" checksum="2933FC4888EDB8E8" modified="2020-08-12" version="1">MRLCLFYLITFFLFLGVQADKGANKRTGEKGGDKGGGSGKFSSNLADADSDKMAKERKEGIAKLWKRWHYQMEKHGYTLPFSKINKDSTAASGAVTPAAADTTTAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VB58</accession>
    <name>A0A6I8VB58_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC26532759</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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            <authorList>
                <consortium name="RefSeq"/>
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            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026347197" evidence="1">
        <location>
            <begin position="23"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015037017.2"/>
        </source>
    </evidence>
    <sequence length="149" mass="17424" checksum="0F0BBBDF68D6781B" modified="2020-08-12" version="1">MKFLLTFLICFWLAPNPPLGSCQTDALIKLELENIRGFANNNPEFTGWRDRLLDALKPISEQSKMQTLQDFQRWNVQRLDLEKQIFHICEKLQKSIMGKCHETFRKLRDACVWSLTKTNTKKADVINVIKKAKCLGMTRGKERDYGLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VEH4</accession>
    <name>A0A6I8VEH4_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Mucin-2</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">LOC26533789</name>
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    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">MV2-25</strain>
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                <consortium name="RefSeq"/>
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            <strain evidence="5">MV-25-SWS-2005</strain>
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        <property type="component" value="Chromosome 3"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chitin-bd_dom"/>
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        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="CBM_14"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026176643" evidence="2">
        <location>
            <begin position="19"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
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            <begin position="520"/>
            <end position="578"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="491"/>
        </location>
    </feature>
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            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
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    </feature>
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        </location>
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            <end position="115"/>
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        </location>
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        </location>
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        </location>
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        </location>
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        <location>
            <begin position="462"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015039595.2"/>
        </source>
    </evidence>
    <sequence length="581" mass="60554" checksum="56129CB336E1A36D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFH9</accession>
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        <submittedName>
            <fullName evidence="10 11">Vigilin isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="10 11" type="primary">Dp1</name>
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    <organism evidence="9 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
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        </source>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
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        </source>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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    <dbReference type="KEGG" id="dpo:4804813"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22405">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22413">
        <property type="entry name" value="KH-I_Vigilin_rpt10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22414">
        <property type="entry name" value="KH-I_Vigilin_rpt11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22415">
        <property type="entry name" value="KH-I_Vigilin_rpt12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22416">
        <property type="entry name" value="KH-I_Vigilin_rpt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22417">
        <property type="entry name" value="KH-I_Vigilin_rpt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22418">
        <property type="entry name" value="KH-I_Vigilin_rpt15"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="DODECA-SATELLITE-BINDING PROTEIN 1, ISOFORM A"/>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
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            <end position="235"/>
        </location>
    </feature>
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            <begin position="239"/>
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        </location>
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        <location>
            <begin position="814"/>
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        <location>
            <begin position="887"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="992"/>
            <end position="1058"/>
        </location>
    </feature>
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        <location>
            <begin position="1070"/>
            <end position="1139"/>
        </location>
    </feature>
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        <location>
            <begin position="1143"/>
            <end position="1214"/>
        </location>
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            <begin position="1"/>
            <end position="59"/>
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    </feature>
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            <begin position="73"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="927"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="1270"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_015040209.1"/>
        </source>
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    <sequence length="1315" mass="144849" checksum="E806B8F1B9EE26AC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJF1</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein RhoU isoform X1</fullName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Diptera</taxon>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">MV-25-SWS-2005</strain>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:cellular process involved in reproduction in multicellular organism"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="RASTRNSFRMNG"/>
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        <location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015042276.2"/>
        </source>
    </evidence>
    <sequence length="600" mass="64262" checksum="A707CAF52F21C71B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJG9</accession>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein Tsen34</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">Tsen34</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="PANTHER" id="PTHR13070:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF26577">
        <property type="entry name" value="TSEN34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TSEN34 N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015042615.2"/>
        </source>
    </evidence>
    <sequence length="189" mass="20975" checksum="0914306EACFC3D17" modified="2020-08-12" version="1">MYLTLLNGTGYAFSVDDYMELRTKHRLMGALVGTANTKGWSPNQSTLPVELTKFETQLILDEGIALLVNKSKTFSTSPTPKELAEYKADLKERLEGQADALKGEKLRETERYMDKILLGKRNKLLKQGLSTEAAALDGEDVLKEVADNFKFDLQNALMEVPCQHLSKHTAEIIPGPIVDTSCISFVKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKQ8</accession>
    <name>A0A6I8VKQ8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015043365.2">
        <property type="nucleotide sequence ID" value="XM_015187879.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VKQ8"/>
    <dbReference type="KEGG" id="dpo:26533620"/>
    <dbReference type="InParanoid" id="A0A6I8VKQ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VKQ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015043365.2"/>
        </source>
    </evidence>
    <sequence length="362" mass="40855" checksum="F69F1C5584235D32" modified="2020-08-12" version="1">MLLNLPLEIVDQLFGYLNEKDQLNFADAHQKLGSALLFHACGRYAKLDCGEFTDDELKVILETCGNSVVKIHIDLLVGTSVIELVPKYCSKLKSAHLCVSNGNITAMKSILSMKDLEFIQIWSPGYVESDLLLDINAGCKRLEICYITNPGVNHLQRLEHLEYLTIYSDRSAGVNHILDVCVHLKKLKNLCVITAEDNTTGSVDHYHDFVYPQVESITFMFCGLCTVLPSCPKLRTLTLFCNEYRNVRIGQMITKYALTLERVTVQLEDAETDGFTAEVFLEVLGECKNLQTISMGAIHVITDVFNEHLDDFINILMANGFNEQRRFLVNTGSSSANEHILKLKTDYINSEIWNLLSFQVND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPR1</accession>
    <name>A0A6I8VPR1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC13036336</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233031.1">
        <property type="nucleotide sequence ID" value="XM_033377140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPR1"/>
    <dbReference type="KEGG" id="dpo:13036336"/>
    <dbReference type="InParanoid" id="A0A6I8VPR1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233031.1"/>
        </source>
    </evidence>
    <sequence length="137" mass="15684" checksum="624A958757DAB4AB" modified="2020-08-12" version="1">MNKPSFQDAVVFKFTNFVCESYNKSFFVFNNCRLKAINRNKVVFNMNATILHPANDITIRCRLFKKANGFKPWIIDRSVDVCRYMRTGYDPYLKIVFGLFHDFSNINHTCPFVTMNMGAGADTGDDGTRVVSEAESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VT58</accession>
    <name>A0A6I8VT58_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5" type="primary">LOC117183672</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="3 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 5">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="3 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234254.1">
        <property type="nucleotide sequence ID" value="XM_033378363.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234255.1">
        <property type="nucleotide sequence ID" value="XM_033378364.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234256.1">
        <property type="nucleotide sequence ID" value="XM_033378365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VT58"/>
    <dbReference type="KEGG" id="dpo:117183672"/>
    <dbReference type="OMA" id="MNDIRFK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033234254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033234256.1"/>
        </source>
    </evidence>
    <sequence length="179" mass="19966" checksum="64ECABE04C9BD625" modified="2020-08-12" version="1">MRVPRHKPFTGSDNCRNSNSNSDSTSISIGSNRNVPQLPSVIVQYILRWPVINPLTFLMNDIRFKSFYRASKTTSAAAAAGGATASKSCDKSQNSNPRTYNNSSKSNKVFLHYIPRDPRLRILHRTAALNKPLNRSRYTLSELNITEDLCNYGELIGGSDTRARSVEKDLVPVKISGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VT97</accession>
    <name>A0A6I8VT97_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183586</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233879.1">
        <property type="nucleotide sequence ID" value="XM_033377988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VT97"/>
    <dbReference type="KEGG" id="dpo:117183586"/>
    <dbReference type="InParanoid" id="A0A6I8VT97"/>
    <dbReference type="OMA" id="VCERLCY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026150607" evidence="1">
        <location>
            <begin position="27"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233879.1"/>
        </source>
    </evidence>
    <sequence length="91" mass="9956" checksum="3BB9D13D5859E5B4" modified="2020-08-12" version="1">MTCSSSLLVLLSMMVLMTALPFEAHAQIVRNGHCRPTPVRVPAGGMVCERQCYTPGGPPFLCRRIPLTTDLRICSGTCCRSGPYCLQLLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTS4</accession>
    <name>A0A6I8VTS4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helicase domino-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183681</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234313.1">
        <property type="nucleotide sequence ID" value="XM_033378422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTS4"/>
    <dbReference type="KEGG" id="dpo:117183681"/>
    <dbReference type="InParanoid" id="A0A6I8VTS4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033234313.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="28429" checksum="7497CC873010CC81" modified="2020-08-12" version="1">MSFNINFNCGPPPVGQNVYKIGELEGEKNIITKESILECINSLPKCENLTLQQKPEVGCQQTANADDSFDCSNLTAAQLEQLALLTAAANPGQSSVSLMVMIPVESLLDIVQVLSGIAAQIVSMSSTVLKGNSSKGTTSEVPIWVDAGVGVPPSVGKIDMEMYQSPADKPSVVVQTQSKSTHNPSFRILEHVIIPSQQPGSIGSLCKQEQQQKATTAGGSAQCYWRVVAVGLRFSSFLFCRCCVDAVGLRFSSFRSFHCRCFVAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWY6</accession>
    <name>A0A6I8VWY6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183963</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235597.1">
        <property type="nucleotide sequence ID" value="XM_033379706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWY6"/>
    <dbReference type="KEGG" id="dpo:117183963"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033235597.1"/>
        </source>
    </evidence>
    <sequence length="177" mass="20488" checksum="CE5AA7142C097CC2" modified="2020-08-12" version="1">MCRLHSKYIEPNINLNISKIATKFEFTNLKCSCPIKEFCTVDYCYLKSVNRTYKYASIRINVFATVTNVEVNGATFKRMNGYKPFLYNVTVDGCRFMENRNRNPVANYLFGFLGKYHNINHTCPYSHDIIIDKLSIGHVNDHMMEFLPFAIGSYLLQASFSIDGIRRLVLQFYGTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VX66</accession>
    <name>A0A6I8VX66_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4818002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235652.1">
        <property type="nucleotide sequence ID" value="XM_033379761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VX66"/>
    <dbReference type="KEGG" id="dpo:4818002"/>
    <dbReference type="InParanoid" id="A0A6I8VX66"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007970">
        <property type="entry name" value="DUF733"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05306">
        <property type="entry name" value="DUF733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235652.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="31007" checksum="426312BDBD0ECF31" modified="2020-08-12" version="1">SVPGVDHKTSTKTGHERKRYHREETQVKLECYGRSCHKHINQQISNNMGNSIDRKVSPTPTPSPIETIKPKEQEQVVEQQPEAPKLDTNLPLQLFCYRQQLPAKNHHTMKWATAKLLLTEELLKLQQTHRPRAPRCPWENYEATMLTDEEDADGNQENRAPNNDDLSRELSTLKSYRMELRETILKVAHKKKTKTSQKKLKRLNRRTLISSKKPSNKAKEPHKPLYNEWLLGEDEPESPLQADSLQDGSPVMVDLPAASARDSLASGGHPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZ04</accession>
    <name>A0A6I8VZ04_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 40</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">l(2)41Ab</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235754.1">
        <property type="nucleotide sequence ID" value="XM_033379863.1"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0273439">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037386">
        <property type="entry name" value="CCDC40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16275">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16275:SF8">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="623"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235754.1"/>
        </source>
    </evidence>
    <sequence length="891" mass="105499" checksum="FBCB35C37F684B35" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W036</accession>
    <name>A0A6I8W036_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ND-13A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the complex I NDUFS6 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236129.1">
        <property type="nucleotide sequence ID" value="XM_033380238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W036"/>
    <dbReference type="FunCoup" id="A0A6I8W036">
        <property type="interactions" value="761"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817019"/>
    <dbReference type="InParanoid" id="A0A6I8W036"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W036">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.40:FF:000003">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.40">
        <property type="entry name" value="q5lls5 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016668">
        <property type="entry name" value="NDUFS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019401">
        <property type="entry name" value="Znf_CHCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13156">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-A SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10276">
        <property type="entry name" value="zf-CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016564">
        <property type="entry name" value="CI-13KD-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger CHCC-type" evidence="12">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033236129.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="14152" checksum="EF1AB4D98984F3DF" modified="2020-08-12" version="1">MASKQLVNNLAKVGRCGQNWLAPLTAVRNNSSRSDIDKVTHTGQVFDKDDYRNVRFVNAKRYVNENWGIKMIEDVPPIESTERVVFCDGGDGPLGHPKVYINLDAPGSHTCGYCGLRFVKKDDHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1Y6</accession>
    <name>A0A6I8W1Y6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4813589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237347.1">
        <property type="nucleotide sequence ID" value="XM_033381456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W1Y6"/>
    <dbReference type="FunCoup" id="A0A6I8W1Y6">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W1Y6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026310963" evidence="2">
        <location>
            <begin position="28"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033237347.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="26533" checksum="D553F5EBD69A7CD7" modified="2020-08-12" version="1">MAVSVALGLRLAPLKAILLCSTSLCSAQNVTYSNDTQEALKLEALLQSSSTTSSTSSTPKPRGCSTSPLTKAKAKQAHEIEEDDNNFSNDRLPALTDDEYSSLGEDANPLHFLKQQPPPQQQPAKQQQPKPEPMAMPQAGGSSIYITIPIYINSAGKLPLTLTIGDQQMSLNQLRKSASGSAAANKKNPHTKAPNSHFNRLLEQIETTPKRRTTNRHRSQLKSNIYAAKERPDRHASVYYPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5H2</accession>
    <name>A0A6I8W5H2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Septin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Septin4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238596.1">
        <property type="nucleotide sequence ID" value="XM_033382705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5H2"/>
    <dbReference type="FunCoup" id="A0A6I8W5H2">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W5H2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081957">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W5H2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000064">
        <property type="entry name" value="Septin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="10">
        <location>
            <begin position="127"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033238596.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="53457" checksum="48EF207AB5445B6E" modified="2020-08-12" version="1">MKHQPPPPPLPLTQPPSLSGGGVGGVSGSVISSSSGSSSIVANAISSLNGNNSATSSSSCIQPANNNNNNHNNNISNISSSRGSSFEAGKCRPPLYPKPKTPSFDKDRDYIGFATLPEQVHRKSVKRGFEFTLMVVGESGLGKSTLINSLFLGDLYKNRQMPNVEERIEKTTRVEKKTMDIEERGVRLRLTVVDTPGFGDAINCEDSWRVCTSYIDEQFRQYFTDESGLNRRNIQDNRVHCCLYFVPPWGHSLRQMDLDLIRRLHRKVNIVLVIGKADCLNKQEVRKLKERILQDLEDNHIQLYQFPECDSDEDDDFKQQDRELKASIPFAVVGSNTILEVAGKKVRGRQYPWGVVNVEDPEHSDFIKLRTFLISTHMQDLKDTTQDVHYENFRAQCISQISQHALRERGKLKRDSISSTNGFDAAISETDRLLLQKDEEIRRMQDMLTQMQEKLKQTHLMEMKKNDSVIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5V8</accession>
    <name>A0A6I8W5V8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RAB6A-GEF complex partner protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4802496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238750.1">
        <property type="nucleotide sequence ID" value="XM_033382859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5V8"/>
    <dbReference type="FunCoup" id="A0A6I8W5V8">
        <property type="interactions" value="2087"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W5V8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W5V8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014848">
        <property type="entry name" value="Rgp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12507">
        <property type="entry name" value="REDUCED GROWTH PHENOTYPE 1 RGP1, YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08737">
        <property type="entry name" value="Rgp1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033238750.1"/>
        </source>
    </evidence>
    <sequence length="452" mass="50586" checksum="2ADEBFB8C576E9D9" modified="2020-08-12" version="1">MIEIKAKLVRADSAIYATGERVECLIEFTHKVFADEQPPSNRNANEAGYEFQPMTRISLLNSIFYSLENLAWASAQLHCYRKTSYSTPAADKTQELTELIGRTALDAVTQAPGEVIVATKPKILFCDLKLQPGETKIYFFNEFVPRDGPPTYRGHDIRYFYKITIATQRVKSKVQTLTVPIRVLPIPIIARPDELPVTVETNEELAPTNPFLEKREISELEISWHHLKNVTARRAPKFYRISNKRGFVGRFCLFKPAYKLGEDIVGSLDFDGCQVRCVQFSVKLQQQELPIRPQPAQALSQSQSSVGDDASSIQSFDMASIASGVAVDSMHRSATSGSAREDTPAPTGKLSTIATTHQVCYATLQTQVVVPIPLHVTPTFRTDLVDVKWRLHFEFVTSTMMDFGGPNPESGELKAPAEMPVETMVWNLPVTIYAANPLQIYNPNQTFNLLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6I7</accession>
    <name>A0A6I8W6I7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neurobeachin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rg</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033238917.1">
        <property type="nucleotide sequence ID" value="XM_033383026.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8W6I7">
        <property type="interactions" value="1304"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W6I7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079851">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W6I7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000059">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000010">
        <property type="entry name" value="neurobeachin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010508">
        <property type="entry name" value="NBEA-like_DUF1088"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046852">
        <property type="entry name" value="Neurobeachin_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF162">
        <property type="entry name" value="NEUROBEACHIN"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000028">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000004">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF9">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="43"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002132399.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="37676" checksum="1D969855161CFD8A" modified="2008-10-14" version="1">MCDNNETALRKPWVEKYRPYSLDDLISHDEIILTINRFISQKQLPHLLFYGPPGTGKTSTILACARQLYPPALFKSMVLELNASDDRGIGIVRGQILNFASTRTIFCDTFKLIILDEADAMTNDAQNALRRIIEKYTDNVRFCIICNYLSKIIPALQSRCTRFRFAPLSPDKMIPRLEQIVQSEDIKITENGKKALLTLSKGDMRKVLNVLQSTAMAFDVVNEDNVYMCAGYPLRQDIENILKALLSGHNFEVSFQNVDVAKSARGLALEDIITELHLIVMRLEFPISVMNELVVKLAHIEERLTKGCTDIANTAALVSAFFICRDMVSLHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>B5DHT1</accession>
    <accession>A0A6I8UX59</accession>
    <name>B5DHT1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sodium channel protein Nach</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ppk13</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132456.2">
        <property type="nucleotide sequence ID" value="XM_002132420.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DHT1"/>
    <dbReference type="FunCoup" id="B5DHT1">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="GeneID" id="6903589"/>
    <dbReference type="KEGG" id="dpo:6903589"/>
    <dbReference type="eggNOG" id="KOG4294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024950_2_0_1"/>
    <dbReference type="InParanoid" id="B5DHT1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246852">
        <property type="expression patterns" value="Expressed in male reproductive system"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000012">
        <property type="entry name" value="Pickpocket 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.470.10:FF:000010">
        <property type="entry name" value="Pickpocket 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF175">
        <property type="entry name" value="PICKPOCKET 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002132456.2"/>
        </source>
    </evidence>
    <sequence length="489" mass="56138" checksum="FEBCA2C99D9D6394" modified="2016-01-20" version="2">MDRSNEQESLARDTVLRSGSLTISFGLEFIERRAAMRFSRSIEEYLSNSTLHGARFIVDKDASWLERIFWVICLVVSWYASWLLIKASLSAFENNAISFVVESSYRDWNTNFPAIIVCESKNMDRIQEVAEQLWGADHDFTLEEVLSEIAFFRGESYHTVHECSGEESTASCFYSNFSYYAQLVRSSCVDSISNCEWNNKPFECCKYFHRMETELGICYAINSLQAGKNKGPKLNMYSNRYTGPGTLTMELHTEATVFILGNEEVPTLVTPKTDFLVVGPYISFTRYISKRDIENDEEIRQTSVYQRNCRFADENILGVHNYYSYSACTVQCRKDRQMELCNCSSHLSPNSPDWQLCNMDGLECLNRNYEDLSVIIAKWSKRRGRKGLVCDCLPSCTEVDITTVSDSKDNMVGEQNPVSRIEVGLIELPTERYKRNLVRGKLDLVVSIGGTTGLFVGASLLSFVEIFYYITIRPYTTYMDDRLKRMVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>B5DJR5</accession>
    <accession>A0A6I8UYW8</accession>
    <name>B5DJR5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein fok isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fok</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133136.1">
        <property type="nucleotide sequence ID" value="XM_002133100.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DJR5"/>
    <dbReference type="GeneID" id="6902610"/>
    <dbReference type="KEGG" id="dpo:6902610"/>
    <dbReference type="eggNOG" id="ENOG502T119">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1972762_0_0_1"/>
    <dbReference type="InParanoid" id="B5DJR5"/>
    <dbReference type="OMA" id="DMHEHEL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250369">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DJR5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133136.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="15517" checksum="A47AC6AFA193B207" modified="2008-10-14" version="1">MDVWGFCVSDSTMSYGGSMRSGYYRDYEQFPYGTLESSSAASSCAVKDSYSRVQEKFKQTKSEKQLRKDCPFCKEHKKRPLINYMRKRQQRKHNDSICEDEEEMHEHEDEGVHQQQQTPTLASVLPTQQSCKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>B5DLB9</accession>
    <accession>A0A6I8UYW7</accession>
    <name>B5DLB9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Gamma-aminobutyric acid receptor-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Atg8a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133691.1">
        <property type="nucleotide sequence ID" value="XM_002133655.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DLB9"/>
    <dbReference type="FunCoup" id="B5DLB9">
        <property type="interactions" value="1566"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DLB9"/>
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    <sequence length="120" mass="14410" checksum="583C03E46320A17A" modified="2008-10-14" version="1">MKFQYKEEHAFEKRRAEGDKIRRKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHEEDYFLYIAYSDENVYGIYYH</sequence>
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    <accession>B5DM91</accession>
    <accession>A0A6I8UZC7</accession>
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            <fullName evidence="4">Transcription initiation factor TFIID subunit 11</fullName>
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        <name evidence="4" type="primary">LOC6901281</name>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
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    </reference>
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        <location>
            <begin position="1"/>
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            <begin position="20"/>
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            <begin position="54"/>
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        <location>
            <begin position="64"/>
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            <begin position="120"/>
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        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_002133982.1"/>
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<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>B5DUB6</accession>
    <accession>A0A6I8V1V4</accession>
    <name>B5DUB6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome c oxidase copper chaperone-like</fullName>
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        <name evidence="11" type="primary">Dpse\GA23194</name>
        <name evidence="10" type="synonym">Dpse\GA29150</name>
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        <name evidence="10" type="ORF">Dpse_GA29150</name>
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    <organism evidence="11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
                <person name="Bradley R.K."/>
                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
                <person name="Castrezana S."/>
                <person name="Celniker S.E."/>
                <person name="Chang J.L."/>
                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
                <person name="Clifton S.W."/>
                <person name="Comeron J.M."/>
                <person name="Costello J.C."/>
                <person name="Coyne J.A."/>
                <person name="Daub J."/>
                <person name="David R.G."/>
                <person name="Delcher A.L."/>
                <person name="Delehaunty K."/>
                <person name="Do C.B."/>
                <person name="Ebling H."/>
                <person name="Edwards K."/>
                <person name="Eickbush T."/>
                <person name="Evans J.D."/>
                <person name="Filipski A."/>
                <person name="Findeiss S."/>
                <person name="Freyhult E."/>
                <person name="Fulton L."/>
                <person name="Fulton R."/>
                <person name="Garcia A.C."/>
                <person name="Gardiner A."/>
                <person name="Garfield D.A."/>
                <person name="Garvin B.E."/>
                <person name="Gibson G."/>
                <person name="Gilbert D."/>
                <person name="Gnerre S."/>
                <person name="Godfrey J."/>
                <person name="Good R."/>
                <person name="Gotea V."/>
                <person name="Gravely B."/>
                <person name="Greenberg A.J."/>
                <person name="Griffiths-Jones S."/>
                <person name="Gross S."/>
                <person name="Guigo R."/>
                <person name="Gustafson E.A."/>
                <person name="Haerty W."/>
                <person name="Hahn M.W."/>
                <person name="Halligan D.L."/>
                <person name="Halpern A.L."/>
                <person name="Halter G.M."/>
                <person name="Han M.V."/>
                <person name="Heger A."/>
                <person name="Hillier L."/>
                <person name="Hinrichs A.S."/>
                <person name="Holmes I."/>
                <person name="Hoskins R.A."/>
                <person name="Hubisz M.J."/>
                <person name="Hultmark D."/>
                <person name="Huntley M.A."/>
                <person name="Jaffe D.B."/>
                <person name="Jagadeeshan S."/>
                <person name="Jeck W.R."/>
                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
                <person name="Li R."/>
                <person name="Lin C.F."/>
                <person name="Lin M.F."/>
                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
                <person name="Machado C.A."/>
                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
                <person name="O'Grady P."/>
                <person name="Pachter L."/>
                <person name="Papaceit M."/>
                <person name="Parisi M.J."/>
                <person name="Parisi M."/>
                <person name="Parts L."/>
                <person name="Pedersen J.S."/>
                <person name="Pesole G."/>
                <person name="Phillippy A.M."/>
                <person name="Ponting C.P."/>
                <person name="Pop M."/>
                <person name="Porcelli D."/>
                <person name="Powell J.R."/>
                <person name="Prohaska S."/>
                <person name="Pruitt K."/>
                <person name="Puig M."/>
                <person name="Quesneville H."/>
                <person name="Ram K.R."/>
                <person name="Rand D."/>
                <person name="Rasmussen M.D."/>
                <person name="Reed L.K."/>
                <person name="Reenan R."/>
                <person name="Reily A."/>
                <person name="Remington K.A."/>
                <person name="Rieger T.T."/>
                <person name="Ritchie M.G."/>
                <person name="Robin C."/>
                <person name="Rogers Y.H."/>
                <person name="Rohde C."/>
                <person name="Rozas J."/>
                <person name="Rubenfield M.J."/>
                <person name="Ruiz A."/>
                <person name="Russo S."/>
                <person name="Salzberg S.L."/>
                <person name="Sanchez-Gracia A."/>
                <person name="Saranga D.J."/>
                <person name="Sato H."/>
                <person name="Schaeffer S.W."/>
                <person name="Schatz M.C."/>
                <person name="Schlenke T."/>
                <person name="Schwartz R."/>
                <person name="Segarra C."/>
                <person name="Singh R.S."/>
                <person name="Sirot L."/>
                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
                <person name="Smith T.F."/>
                <person name="Spieth J."/>
                <person name="Stage D.E."/>
                <person name="Stark A."/>
                <person name="Stephan W."/>
                <person name="Strausberg R.L."/>
                <person name="Strempel S."/>
                <person name="Sturgill D."/>
                <person name="Sutton G."/>
                <person name="Sutton G.G."/>
                <person name="Tao W."/>
                <person name="Teichmann S."/>
                <person name="Tobari Y.N."/>
                <person name="Tomimura Y."/>
                <person name="Tsolas J.M."/>
                <person name="Valente V.L."/>
                <person name="Venter E."/>
                <person name="Venter J.C."/>
                <person name="Vicario S."/>
                <person name="Vieira F.G."/>
                <person name="Vilella A.J."/>
                <person name="Villasante A."/>
                <person name="Walenz B."/>
                <person name="Wang J."/>
                <person name="Wasserman M."/>
                <person name="Watts T."/>
                <person name="Wilson D."/>
                <person name="Wilson R.K."/>
                <person name="Wing R.A."/>
                <person name="Wolfner M.F."/>
                <person name="Wong A."/>
                <person name="Wong G.K."/>
                <person name="Wu C.I."/>
                <person name="Wu G."/>
                <person name="Yamamoto D."/>
                <person name="Yang H.P."/>
                <person name="Yang S.P."/>
                <person name="Yorke J.A."/>
                <person name="Yoshida K."/>
                <person name="Zdobnov E."/>
                <person name="Zhang P."/>
                <person name="Zhang Y."/>
                <person name="Zimin A.V."/>
                <person name="Baldwin J."/>
                <person name="Abdouelleil A."/>
                <person name="Abdulkadir J."/>
                <person name="Abebe A."/>
                <person name="Abera B."/>
                <person name="Abreu J."/>
                <person name="Acer S.C."/>
                <person name="Aftuck L."/>
                <person name="Alexander A."/>
                <person name="An P."/>
                <person name="Anderson E."/>
                <person name="Anderson S."/>
                <person name="Arachi H."/>
                <person name="Azer M."/>
                <person name="Bachantsang P."/>
                <person name="Barry A."/>
                <person name="Bayul T."/>
                <person name="Berlin A."/>
                <person name="Bessette D."/>
                <person name="Bloom T."/>
                <person name="Blye J."/>
                <person name="Boguslavskiy L."/>
                <person name="Bonnet C."/>
                <person name="Boukhgalter B."/>
                <person name="Bourzgui I."/>
                <person name="Brown A."/>
                <person name="Cahill P."/>
                <person name="Channer S."/>
                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
                <person name="Costello M."/>
                <person name="D'Aco K."/>
                <person name="Daza R."/>
                <person name="De Haan G."/>
                <person name="DeGray S."/>
                <person name="DeMaso C."/>
                <person name="Dhargay N."/>
                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
                <person name="Dorjee K."/>
                <person name="Dupes A."/>
                <person name="Elong R."/>
                <person name="Falk J."/>
                <person name="Farina A."/>
                <person name="Faro S."/>
                <person name="Ferguson D."/>
                <person name="Fisher S."/>
                <person name="Foley C.D."/>
                <person name="Franke A."/>
                <person name="Friedrich D."/>
                <person name="Gadbois L."/>
                <person name="Gearin G."/>
                <person name="Gearin C.R."/>
                <person name="Giannoukos G."/>
                <person name="Goode T."/>
                <person name="Graham J."/>
                <person name="Grandbois E."/>
                <person name="Grewal S."/>
                <person name="Gyaltsen K."/>
                <person name="Hafez N."/>
                <person name="Hagos B."/>
                <person name="Hall J."/>
                <person name="Henson C."/>
                <person name="Hollinger A."/>
                <person name="Honan T."/>
                <person name="Huard M.D."/>
                <person name="Hughes L."/>
                <person name="Hurhula B."/>
                <person name="Husby M.E."/>
                <person name="Kamat A."/>
                <person name="Kanga B."/>
                <person name="Kashin S."/>
                <person name="Khazanovich D."/>
                <person name="Kisner P."/>
                <person name="Lance K."/>
                <person name="Lara M."/>
                <person name="Lee W."/>
                <person name="Lennon N."/>
                <person name="Letendre F."/>
                <person name="LeVine R."/>
                <person name="Lipovsky A."/>
                <person name="Liu X."/>
                <person name="Liu J."/>
                <person name="Liu S."/>
                <person name="Lokyitsang T."/>
                <person name="Lokyitsang Y."/>
                <person name="Lubonja R."/>
                <person name="Lui A."/>
                <person name="MacDonald P."/>
                <person name="Magnisalis V."/>
                <person name="Maru K."/>
                <person name="Matthews C."/>
                <person name="McCusker W."/>
                <person name="McDonough S."/>
                <person name="Mehta T."/>
                <person name="Meldrim J."/>
                <person name="Meneus L."/>
                <person name="Mihai O."/>
                <person name="Mihalev A."/>
                <person name="Mihova T."/>
                <person name="Mittelman R."/>
                <person name="Mlenga V."/>
                <person name="Montmayeur A."/>
                <person name="Mulrain L."/>
                <person name="Navidi A."/>
                <person name="Naylor J."/>
                <person name="Negash T."/>
                <person name="Nguyen T."/>
                <person name="Nguyen N."/>
                <person name="Nicol R."/>
                <person name="Norbu C."/>
                <person name="Norbu N."/>
                <person name="Novod N."/>
                <person name="O'Neill B."/>
                <person name="Osman S."/>
                <person name="Markiewicz E."/>
                <person name="Oyono O.L."/>
                <person name="Patti C."/>
                <person name="Phunkhang P."/>
                <person name="Pierre F."/>
                <person name="Priest M."/>
                <person name="Raghuraman S."/>
                <person name="Rege F."/>
                <person name="Reyes R."/>
                <person name="Rise C."/>
                <person name="Rogov P."/>
                <person name="Ross K."/>
                <person name="Ryan E."/>
                <person name="Settipalli S."/>
                <person name="Shea T."/>
                <person name="Sherpa N."/>
                <person name="Shi L."/>
                <person name="Shih D."/>
                <person name="Sparrow T."/>
                <person name="Spaulding J."/>
                <person name="Stalker J."/>
                <person name="Stange-Thomann N."/>
                <person name="Stavropoulos S."/>
                <person name="Stone C."/>
                <person name="Strader C."/>
                <person name="Tesfaye S."/>
                <person name="Thomson T."/>
                <person name="Thoulutsang Y."/>
                <person name="Thoulutsang D."/>
                <person name="Topham K."/>
                <person name="Topping I."/>
                <person name="Tsamla T."/>
                <person name="Vassiliev H."/>
                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
                <person name="Weiand M."/>
                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
                <person name="Yu Q."/>
                <person name="Zembek L."/>
                <person name="Zhong D."/>
                <person name="Zimmer A."/>
                <person name="Zwirko Z."/>
                <person name="Jaffe D.B."/>
                <person name="Alvarez P."/>
                <person name="Brockman W."/>
                <person name="Butler J."/>
                <person name="Chin C."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Kleber M."/>
                <person name="Mauceli E."/>
                <person name="MacCallum I."/>
            </authorList>
            <dbReference type="PubMed" id="17994087"/>
            <dbReference type="DOI" id="10.1038/nature06341"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
            <authorList>
                <person name="English A.C."/>
                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COX17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH674075">
        <property type="protein sequence ID" value="EDY71596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CH673223">
        <property type="protein sequence ID" value="EDY71953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002136481.1">
        <property type="nucleotide sequence ID" value="XM_002136445.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DUB6"/>
    <dbReference type="STRING" id="46245.B5DUB6"/>
    <dbReference type="GeneID" id="6904037"/>
    <dbReference type="KEGG" id="dpo:6904037"/>
    <dbReference type="eggNOG" id="KOG3496">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149618_2_0_1"/>
    <dbReference type="OMA" id="QEACTKW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 8" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EDY71596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EDY71953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002136481.1"/>
        </source>
    </evidence>
    <sequence length="75" mass="7878" checksum="9B487DC0309BFD82" modified="2008-10-14" version="1">MGSSTSKGVEVAQSAASEPVAATNGGGAKPKCNACCVCPETKRARDQCMVERGEENCTEQIEAHKKCMRNAGFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DX5</accession>
    <accession>A0A6I8VLL3</accession>
    <name>Q29DX5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7 8">Uncharacterized protein loaf</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8" type="primary">loaf</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7 8">MV-25-SWS-2005</strain>
            <tissue evidence="6 7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352788.3">
        <property type="nucleotide sequence ID" value="XM_001352752.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043855.1">
        <property type="nucleotide sequence ID" value="XM_015188369.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241150.1">
        <property type="nucleotide sequence ID" value="XM_033385259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DX5"/>
    <dbReference type="GeneID" id="4812059"/>
    <dbReference type="KEGG" id="dpo:4812059"/>
    <dbReference type="eggNOG" id="ENOG502QWHA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062511_1_0_1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079302">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DX5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042333">
        <property type="entry name" value="LRAD2/Mig-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24652">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR CLASS A DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352788.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015043855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033241150.1"/>
        </source>
    </evidence>
    <sequence length="358" mass="39529" checksum="1316C66E3C06FCEC" modified="2016-01-20" version="3">MLLMPTSCGPGPPAGPNGAGADGGGPAEVARRRTAASGSPKWLLMLLMLFAILARMPHSQVAAEKSKHYYMQSLCKNHFLQQLYRKIDGAVLWSQNERNLDCIITFQTHSVLQRFMLRFDMLQLDCNDHLLVYDGAHAVSTPKIDISCRNTKNTVNALLTRTNFVTLKYVTDGWGTDVNGFKLVITSVKDPKHTCKDFTCATREFCIHPDLLCDGVNHCGDNSDESVQNLCQSEASSTVFGMDMTWFVLIVVGVLLVLSALIVAVAICVCRRQDENASNQNTALQLHHNADTNGSSKHLHYHGISVGGTLTREHTQLPPQSGNWHHPAGPSGYHRIPHNYLKMATKVRPIWCLANSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DY4</accession>
    <accession>A0A6I8UBF2</accession>
    <name>Q29DY4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RpL10Ab</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352779.1">
        <property type="nucleotide sequence ID" value="XM_001352743.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DY4"/>
    <dbReference type="SMR" id="Q29DY4"/>
    <dbReference type="FunCoup" id="Q29DY4">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DY4"/>
    <dbReference type="GeneID" id="4812231"/>
    <dbReference type="KEGG" id="dpo:4812231"/>
    <dbReference type="eggNOG" id="KOG1570">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_3_0_1"/>
    <dbReference type="InParanoid" id="Q29DY4"/>
    <dbReference type="OMA" id="GPRNKMP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080231">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000006">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000002">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000009">
        <property type="entry name" value="Ribosomal protein L10a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352779.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="24303" checksum="7DB63E790752016F" modified="2006-04-04" version="1">MASKVSRDTLYEGVSGILENSLKKKRGFLETVELQIGLKNYDPQKDKRFSGTVKLKHIPRPKMKVCILGDQQHCDEAKANGVEFMDAEALKKLNKNKKLVKKLAKSYDAFLASESLIKQIPRLLGPGLNKAGKFPALLSHQESMIGKIEEVKSTIKFQMKKVLCLSVAVGHVGMKSDELAQNVNLSINFLVSLLKKNWQNVRSLHIKSSMGPPQRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GK0</accession>
    <accession>A0A6I8VID8</accession>
    <name>Q29GK0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein NipSnap</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Nipsnap</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NipSnap family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041978.1">
        <property type="nucleotide sequence ID" value="XM_015186492.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GK0"/>
    <dbReference type="FunCoup" id="Q29GK0">
        <property type="interactions" value="1415"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GK0"/>
    <dbReference type="GeneID" id="4814663"/>
    <dbReference type="KEGG" id="dpo:4814663"/>
    <dbReference type="eggNOG" id="KOG2883">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053393_1_0_1"/>
    <dbReference type="InParanoid" id="Q29GK0"/>
    <dbReference type="OMA" id="REKSWSV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081602">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29GK0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000046">
        <property type="entry name" value="Nipsnap, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000003">
        <property type="entry name" value="Protein NipSnap homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051557">
        <property type="entry name" value="NipSnap_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017">
        <property type="entry name" value="NIPSNAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017:SF17">
        <property type="entry name" value="PROTEIN NIPSNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIPSNAP" evidence="2">
        <location>
            <begin position="61"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="NIPSNAP" evidence="2">
        <location>
            <begin position="175"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015041978.1"/>
        </source>
    </evidence>
    <sequence length="274" mass="32046" checksum="5A61ADCFAA191A4C" modified="2006-04-04" version="1">MLKLRNILSVGNNNGLSAVRWISTTANCREADSWFSKLLVRKIEPTKESHSRMLSDKEIIYALHTHNVRPDSMSAYLNNYKTTVSLMNDKKANLSCNLVASWSVQVGDMDQCLHLWKYTGGFEKIDQAKEDLWNDPEYLSLMQERSKFLRSRHLQYLLAFSYWPQIASRSGKNIYEMRSYRLTPGTMIEWGNNWARAINYRKHNNEAFAGFFSQIGRLYNVHHIWCYSSLQDRKETREAAWRSPGWDECVAYTVPLIRDMHCRVLAPTEFSPTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HG9</accession>
    <name>LST8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Protein LST8 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA15597</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EAL31789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HG9"/>
    <dbReference type="SMR" id="Q29HG9"/>
    <dbReference type="FunCoup" id="Q29HG9">
        <property type="interactions" value="1149"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HG9"/>
    <dbReference type="eggNOG" id="KOG0315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_5_1"/>
    <dbReference type="InParanoid" id="Q29HG9"/>
    <dbReference type="OMA" id="VQRNYKH"/>
    <dbReference type="PhylomeDB" id="Q29HG9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032535">
        <property type="term" value="P:regulation of cellular component size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000505">
        <property type="entry name" value="Blast:Protein LST8 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0853">WD repeat</keyword>
    <feature type="chain" id="PRO_0000326506" description="Protein LST8 homolog">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 2">
        <location>
            <begin position="33"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 3">
        <location>
            <begin position="76"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 4">
        <location>
            <begin position="119"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 5">
        <location>
            <begin position="161"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 6">
        <location>
            <begin position="211"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 7">
        <location>
            <begin position="253"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 8">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="315" mass="35522" checksum="54FDA305906C3813" modified="2006-04-04" version="1">MGDQLILATGGYDHTIKVWQAHTGNCIKTMRFVETSQVNALDRTPDKTRLAACGYQCIRLYDLESNCTAPVINFDGVQKNVTRLGFQEDGNWMFTAGEDHHVRIWDMISAPPHCSRVFDCESPVNAACLHPNQVEIGMGSQNGNVFLWDVKSEKHECIVPEVDASIQDVAISPDGHYLAAANNKGNCYIWSLCSSPDQKMSTMRPTKKIQAHTRYILRCKFSPDSRLLLTTSGDGTACLWKTSDFSKWRELCIENYWVWDAAFSADSKWLFTASSDGVARLWKLETKTPTREYTGHTKAITALSFKDEIIRKVNH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-12-04" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JQ0</accession>
    <name>RETM_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Protein real-time</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">retm</name>
        <name type="ORF">GA21858</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL32911" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CH379062">
        <property type="protein sequence ID" value="EAL32911.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JQ0"/>
    <dbReference type="SMR" id="Q29JQ0"/>
    <dbReference type="FunCoup" id="Q29JQ0">
        <property type="interactions" value="1389"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JQ0"/>
    <dbReference type="eggNOG" id="KOG1471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023840_0_0_1"/>
    <dbReference type="InParanoid" id="Q29JQ0"/>
    <dbReference type="OMA" id="AEWTCFD"/>
    <dbReference type="PhylomeDB" id="Q29JQ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.680:FF:000009">
        <property type="entry name" value="Blast:Protein real-time"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000006">
        <property type="entry name" value="SEC14-like lipid binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051064">
        <property type="entry name" value="SEC14/CRAL-TRIO_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324:SF66">
        <property type="entry name" value="PROTEIN REAL-TIME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324">
        <property type="entry name" value="SEC14 RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000312684" description="Protein real-time">
        <location>
            <begin position="1"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="PRELI/MSF1" evidence="4">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="3">
        <location>
            <begin position="284"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="2">
        <location>
            <begin position="522"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9VMD6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EAL32911.1"/>
        </source>
    </evidence>
    <sequence length="669" mass="76848" checksum="2ED70CC84D19CBCC" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KI1</accession>
    <accession>A0A6I8UIQ1</accession>
    <name>Q29KI1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein Mabiki</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4816312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356134.2">
        <property type="nucleotide sequence ID" value="XM_001356098.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KI1"/>
    <dbReference type="SMR" id="Q29KI1"/>
    <dbReference type="STRING" id="46245.Q29KI1"/>
    <dbReference type="GeneID" id="4816312"/>
    <dbReference type="KEGG" id="dpo:4816312"/>
    <dbReference type="eggNOG" id="ENOG502T6VC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095450_1_0_1"/>
    <dbReference type="InParanoid" id="Q29KI1"/>
    <dbReference type="OMA" id="YAHKKFS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356134.2"/>
        </source>
    </evidence>
    <sequence length="193" mass="22975" checksum="C2373E7FF86E08D8" modified="2008-10-14" version="2">MENYNHKKFTGALKRKRCQEEESDDDVVFIMEQCGHRTPEQPQPKRRFFRPWLDESLAQEQEPQPTRTRQSHPGSPASPPLVDATSTYHANMVRSHTPRQRSPQEQLRRDRNTLASLRHRRSKQQEQALMEQQYFEYRRQHEANLQQQVRLSLYYVRFLQQTMAATREPLQASSHQQMPKAQTTTQHSWGVHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q29L22</accession>
    <accession>A0A6I8UI95</accession>
    <name>Q29L22_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein l(2)05714</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">l(2)05714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355943.2">
        <property type="nucleotide sequence ID" value="XM_001355907.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29L22"/>
    <dbReference type="FunCoup" id="Q29L22">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="GeneID" id="4816729"/>
    <dbReference type="KEGG" id="dpo:4816729"/>
    <dbReference type="eggNOG" id="ENOG502TB9F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064811_0_0_1"/>
    <dbReference type="InParanoid" id="Q29L22"/>
    <dbReference type="OMA" id="QKYREDW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081378">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355943.2"/>
        </source>
    </evidence>
    <sequence length="252" mass="29222" checksum="CB4A461A433531CC" modified="2008-10-14" version="2">MKGIKSERGAPFNTSKVKRLYHQRYRAEWERIPSFSLWLRPSNDGYSAQCQICDATVLARLASVKQHHNTRKHQESMKCNEFLSMNMIKAEDGIIPNEPKPKPKPKAKAKPKMKIEKIEAVEDHEMASNDDSILDDLLGNESLERAYLSEGENVDEREEQHLGEPETEELETEMIQMKHDDVNGVQLEDMHEVSIPEENIMSEFDLFGKSVSLQLNHMDLEDALMCQERLQVVLTEYRLKILKRNREAKRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MJ8</accession>
    <accession>A0A6I8UJS0</accession>
    <name>Q29MJ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Golgi-associated plant pathogenesis-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4816841</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356631.2">
        <property type="nucleotide sequence ID" value="XM_001356595.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MJ8"/>
    <dbReference type="SMR" id="Q29MJ8"/>
    <dbReference type="FunCoup" id="Q29MJ8">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="GeneID" id="4816841"/>
    <dbReference type="KEGG" id="dpo:4816841"/>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035730_9_4_1"/>
    <dbReference type="InParanoid" id="Q29MJ8"/>
    <dbReference type="OMA" id="RRADRTW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078080">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05382">
        <property type="entry name" value="CAP_GAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000010">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034113">
        <property type="entry name" value="SCP_GAPR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356631.2"/>
        </source>
    </evidence>
    <sequence length="171" mass="19254" checksum="BE52AF36EE1E8BFD" modified="2008-10-14" version="2">MSKKPAPIPKTQPRAPGPRGQDTKANNDLFLKEVFNATNKCRGLHGCPALTINNDLNKIAQEWANHLRDKNVMEHRPNPKYGENIFLSGGMDVSGDLPVDMWYREINAYNFDKAEFTPTSGHFTQLIWKASKEMGSGVARKADRTWVVCNYNPPGNVVGQFRDNVPKKLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q29ND6</accession>
    <accession>A0A6I8UJF2</accession>
    <name>Q29ND6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RpS2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356343.1">
        <property type="nucleotide sequence ID" value="XM_001356307.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29ND6"/>
    <dbReference type="FunCoup" id="Q29ND6">
        <property type="interactions" value="1560"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29ND6"/>
    <dbReference type="GeneID" id="4816899"/>
    <dbReference type="KEGG" id="dpo:4816899"/>
    <dbReference type="eggNOG" id="KOG0877">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_0_2_1"/>
    <dbReference type="InParanoid" id="Q29ND6"/>
    <dbReference type="OMA" id="PYEEWSD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079226">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000133">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356343.1"/>
        </source>
    </evidence>
    <sequence length="268" mass="28725" checksum="99C5E639A682D156" modified="2006-04-04" version="1">MADAAPARGGFRGGFGSRGGRGGRGRGRGRGARGRGGKEDSKEWVPVTKLGRLVREGKIKSLEEIYLYSLPIKEFEIIDFFLGSALRDEVLKIMPVQKQTRAGQRTRFKAFVAIGDNNGHIGLGVKCSKEVATAIRGAIILAKLSVVPVRRGYWGNKIGKPHTVPCKVTGKCGSVSVRLIPAPRGTGIVSAPVPKKLLTMAGIEDCYTSARGSTGTLGNFAKATYAAIAKTYAYLTPDLWKEMPLGSTPYQAYSDFLSKPTARLHADA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ79</accession>
    <name>TFB1M_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Dimethyladenosine transferase 1, mitochondrial</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Mitochondrial 12S rRNA dimethylase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Mitochondrial transcription factor B1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtTFB1</name>
        <name type="ORF">GA20255</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="CH379069">
        <property type="protein sequence ID" value="EAL29629.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ79"/>
    <dbReference type="SMR" id="Q2LZ79"/>
    <dbReference type="FunCoup" id="Q2LZ79">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZ79"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0275748">
        <property type="protein sequence ID" value="FBpp0274186"/>
        <property type="gene ID" value="FBgn0080250"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813779"/>
    <dbReference type="eggNOG" id="KOG0821">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_7_0_1"/>
    <dbReference type="InParanoid" id="Q2LZ79"/>
    <dbReference type="OMA" id="RIEQPFK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080250">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034246">
        <property type="term" value="F:mitochondrial transcription factor activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.100:FF:000006">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000485">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF17">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000273184" description="Dimethyladenosine transferase 1, mitochondrial">
        <location>
            <begin position="85"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <sequence length="337" mass="38581" checksum="236CCA329B06C531" modified="2009-03-03" version="2" precursor="true">MSQVTARVLNSGMRLPPLPTIRELVKLYKLQAMKQLSQNFLMDERLTDKIVKSAGRIDPRDIVLEVGPGPGGITRSILRRQPQRLILVEKDRRFGETLQLLRECASPLDMQVDIYYDDILRFNIEQHIPDTTQRIHLIGNLPFSISTRLLINWYADLAARRGAFRRNDTCMTLTFQKEVAERICAPVGSEQRCRLSVMSQIWTEPVMKFIIPGKAFVPKPQVDVGVVKLIPLKQPKTQLPFSLVERVVRHIFSMRQKYCRRGYGTLLPPDSREETTQTLFQRADVRDTLRSFELSVPQCLRLADAYAEYIKEHPEVEAYDYRGPKNPDVSGAAAASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZQ7</accession>
    <accession>A0A6I8UDH0</accession>
    <name>Q2LZQ7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TPPP family protein CG45057</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4813140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353716.1">
        <property type="nucleotide sequence ID" value="XM_001353680.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZQ7"/>
    <dbReference type="GeneID" id="4813140"/>
    <dbReference type="KEGG" id="dpo:4813140"/>
    <dbReference type="eggNOG" id="KOG4070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171300_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LZQ7"/>
    <dbReference type="OMA" id="LFMVYAN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079798">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13638" checksum="9C07BB34194D24A5" modified="2006-02-21" version="1">MADDDDKPKKHTLDTLFIVYCNYKVISTELENEEFDCILLSQIDAWIEQAKLMPNPITRTQTGLLYMRYKKWRLEFEDFVELLTQLASENDLVFDEFKQLLVEAGPPTGATDIVMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZU2</accession>
    <accession>A0A6I8UDE9</accession>
    <name>Q2LZU2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrator complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">IntS10</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Integrator subunit 10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353681.1">
        <property type="nucleotide sequence ID" value="XM_001353645.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZU2"/>
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        <property type="interactions" value="1676"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZU2"/>
    <dbReference type="GeneID" id="4813114"/>
    <dbReference type="KEGG" id="dpo:4813114"/>
    <dbReference type="eggNOG" id="ENOG502QQ28">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023740_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LZU2"/>
    <dbReference type="OMA" id="FYVKMFQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070890">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026164">
        <property type="entry name" value="Int_cplx_su10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16055">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16055:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21045">
        <property type="entry name" value="INT10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="552"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="552"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353681.1"/>
        </source>
    </evidence>
    <sequence length="631" mass="71905" checksum="FC8456B435ECA546" modified="2006-02-21" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NSV8</accession>
    <accession>A0A6I8W0X4</accession>
    <name>A0A0R3NSV8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC13036414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236996.1">
        <property type="nucleotide sequence ID" value="XM_033381105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NSV8"/>
    <dbReference type="STRING" id="46245.A0A0R3NSV8"/>
    <dbReference type="KEGG" id="dpo:13036414"/>
    <dbReference type="eggNOG" id="ENOG502T95Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3NSV8"/>
    <dbReference type="OMA" id="QYDRQVC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0283544">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NSV8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236996.1"/>
        </source>
    </evidence>
    <sequence length="166" mass="18752" checksum="64A19616D9E23454" modified="2016-01-20" version="1">MKYHRVMNKRKTNQTPSPARESEVNIHKQDFDGRISYGALEMEAAAALLMLRYQYDRQSVPMPSTSVTRSPTPPQHIADKLTPKDQAVRTPDSAQPIKKRSIPNHLLRRSLTPAKSTTNAGGSSSIVKAKSRLTPVPRRSENDCNKVLLKSCRNMIREFLDNQELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NTS7</accession>
    <accession>A0A6I8V992</accession>
    <name>A0A0R3NTS7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein 239-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6903239</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015036440.1">
        <property type="nucleotide sequence ID" value="XM_015180954.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NTS7"/>
    <dbReference type="SMR" id="A0A0R3NTS7"/>
    <dbReference type="GeneID" id="6903239"/>
    <dbReference type="KEGG" id="dpo:6903239"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3NTS7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246513">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000450">
        <property type="entry name" value="PR domain zinc finger protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000075">
        <property type="entry name" value="Putative zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001872">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000290">
        <property type="entry name" value="Zinc finger protein 697 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015036440.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="44142" checksum="706BADFB776A69E7" modified="2016-01-20" version="1">MSTRFPTMEEMCRVCMGNSGALANIFDETHTWDTCIADMIAQCTGYEVRRGDLLSENICPPCLEDAVNAFNLIKTYEQSHQLYFPVMEDDIEEDLCYNLEDEKWQMSASGSERSNHFKAEAMMFKCPHCPMSFKQKFHLQRHVQMHTNERPYQCSHCSKSFIQKSNLESHTRNHTGDRPYQCSHCTKSFQQKSHLQVHNRTHTGERPYKCTYCSKSFTRSDILQSHIRTHTGYRPYKCSLCSKSFIQNSDLQVHIRTHTGERPYKCTLCPKSFNQNYYLQMHTRTHTGELSPHQCSYCSKSFSKRGNLLTHIRTHSDERPYKCSYCPESFKLKGILKRHTLIHTKNVSSFPLSETLDNISASTLSVDTTISPAATLYNDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAR9</accession>
    <name>A0A6I8UAR9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Uncharacterized protein miple2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">miple2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pleiotrophin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352491.3">
        <property type="nucleotide sequence ID" value="XM_001352455.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239535.1">
        <property type="nucleotide sequence ID" value="XM_033383644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAR9"/>
    <dbReference type="KEGG" id="dpo:4812169"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAR9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048332">
        <property type="term" value="P:mesoderm morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.90.10:FF:000001">
        <property type="entry name" value="Pleiotrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.90.10">
        <property type="entry name" value="Heparin-binding Growth Factor, Midkine, Chain A- C-terminal Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020090">
        <property type="entry name" value="PTN/MK_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038130">
        <property type="entry name" value="PTN/MK_C_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21050">
        <property type="entry name" value="MIDKINE AND PLEIOTROPHIN 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21050:SF1">
        <property type="entry name" value="MIDKINE AND PLEIOTROPHIN 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01091">
        <property type="entry name" value="PTN_MK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634415" evidence="7">
        <location>
            <begin position="31"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Pleiotrophin/Midkine C-terminal" evidence="8">
        <location>
            <begin position="162"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="60"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="232"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="267"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001352491.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033239535.1"/>
        </source>
    </evidence>
    <sequence length="286" mass="31193" checksum="F1E2D3C58EDB9555" modified="2020-08-12" version="1">MNLIVTSASALTVLNFVLLVLADIPSLINGEEVSDGVRLVMKTRSGDTLVRAARGALPEEAVSPATNVDGQRMRNNRKSNRSQDNLQQAGGHKSRTGHKKHAVAENASAVAQHPAQPKHKQGHKNSDKQQRQVIKQQQHGHQSHPGGKRPGPKAKASETASTCRYAKSAWSNCDAKSNMRTRILSLKKGEQNCLPTRTIQKKCKKGCRYEKGTWTPCIAGQMTREDKLQAEGVGASDQNCDPVRTVNKKCKGTGSSPEGKHHGPNRAAKERKQKDKGTPRRTAPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBE7</accession>
    <name>A0A6I8UBE7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Osteopetrosis-associated transmembrane protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4812425</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352856.4">
        <property type="nucleotide sequence ID" value="XM_001352820.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBE7"/>
    <dbReference type="FunCoup" id="A0A6I8UBE7">
        <property type="interactions" value="939"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812425"/>
    <dbReference type="InParanoid" id="A0A6I8UBE7"/>
    <dbReference type="OMA" id="LKCCQRE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073431">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBE7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019172">
        <property type="entry name" value="Osteopetrosis-assoc_TM_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15644">
        <property type="entry name" value="OSTEOPETROSIS ASSOCIATED TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15644:SF2">
        <property type="entry name" value="OSTEOPETROSIS-ASSOCIATED TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09777">
        <property type="entry name" value="OSTMP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026063695" evidence="3">
        <location>
            <begin position="21"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001352856.4"/>
        </source>
    </evidence>
    <sequence length="292" mass="32104" checksum="71B76659AD172611" modified="2020-08-12" version="1">MSLLFAFLLILSCNCSLLLAKNKSCSLLVGELGDGQSKFVYCATTKSVPVRLCIGCGPEYRILTSSYDALLSQENCSKIYLNSDRINVVATTQGILTNLWQKANCEDCFNGNHSDLYDVKWNILNGCLSVHKDDPCISCKNDYIALNQFYLEMEKANKGHVCFDLQDSMNRTRSNWSKTLGCCHRDVKLNTFLIAVCVVVLLPIVTFYITVIVLTKRREANHGLLNEETPELDAPSTSALITAAVLSTPTENPVVVADRTEKIAKLLSGARAGSDSDLDSSDDEISVKPKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDF4</accession>
    <name>A0A6I8UDF4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cytochrome P450 307a1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">spo</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353744.3">
        <property type="nucleotide sequence ID" value="XM_001353708.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8UDF4"/>
    <dbReference type="FunCoup" id="A0A6I8UDF4">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813730"/>
    <dbReference type="InParanoid" id="A0A6I8UDF4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20617">
        <property type="entry name" value="CYP1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000072">
        <property type="entry name" value="3-hydroxyphenylacetate 6 hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303:SF31">
        <property type="entry name" value="CYTOCHROME P450 307A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24303">
        <property type="entry name" value="HEME-BINDING MONOOXYGENASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="438"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="489"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001353744.3"/>
        </source>
    </evidence>
    <sequence length="542" mass="62357" checksum="07B83323CC3AC463" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEH7</accession>
    <name>A0A6I8UEH7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 8 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Vps8</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354229.3">
        <property type="nucleotide sequence ID" value="XM_001354193.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEH7"/>
    <dbReference type="FunCoup" id="A0A6I8UEH7">
        <property type="interactions" value="1894"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814098"/>
    <dbReference type="InParanoid" id="A0A6I8UEH7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UEH7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="1150"/>
            <end position="1192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001354229.3"/>
        </source>
    </evidence>
    <sequence length="1239" mass="139168" checksum="1E022FE17C6D83DB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEM2</accession>
    <name>A0A6I8UEM2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ganglioside-induced differentiation-associated protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Gdap1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354279.3">
        <property type="nucleotide sequence ID" value="XM_001354243.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEM2"/>
    <dbReference type="FunCoup" id="A0A6I8UEM2">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814142"/>
    <dbReference type="InParanoid" id="A0A6I8UEM2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UEM2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000394">
        <property type="entry name" value="Gdap1, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44188">
        <property type="entry name" value="GDAP1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44188:SF1">
        <property type="entry name" value="GDAP1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="27"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="165"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001354279.3"/>
        </source>
    </evidence>
    <sequence length="330" mass="37751" checksum="55F0BBF35EB5CDFF" modified="2020-08-12" version="1">MGDQAKEIEAAATPILQDFKAPVFTDNKLVLYFHPYNFYSQKVLLVLYEKNIDFTPYVVDLCNGEQYSSWFLNLNPKGDVPVLQDGAFIIPNSTHIINYVESKFRGANHPVLKPEQSSSQYDQVMVYENAVGRVPVGALSLGSFVHDDLKLVPKAPFIGPVRQSCLKNNDKVLDLLRRSVDELESNKSALKQKLDIQIRRKDLVSCRDDFQRVLDAVRHVLLYVEQELSKKAPRNEWLTGDEVTLADISFGLLLHRLYQLGFENYYWSFGKLPQVEGYFLRFKQRQSYHKLQPSNFEILKEMWAKTPGNYKLGAGAGFLGMAMFAAFAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF40</accession>
    <name>A0A6I8UF40_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4814299</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354437.2">
        <property type="nucleotide sequence ID" value="XM_001354401.3"/>
    </dbReference>
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        <property type="interactions" value="1144"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814299"/>
    <dbReference type="InParanoid" id="A0A6I8UF40"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UF40">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="149"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001354437.2"/>
        </source>
    </evidence>
    <sequence length="362" mass="38469" checksum="2339313941909405" modified="2020-08-12" version="1">METPTADDLLQFRDYSATSATNSGTPAQINVNSPTHASGGGGSGIGSGFVGAGGSSSAQRQRGDPLADLIYDMSTSAHAMAGGGATNLQNAPIDGSGGAGSGGGSGARLSFLTIEYYQQFFNVDTYMVLERIANSMIPKRAAGNYLRMNIGENPDLYGPFWITVTLIFSIAISGNIASYLHHASDGYHWHYNFHLVSYAATCIFLYANILPVILWALFKYSLKPIDDADAVETDSATYTPTLLSLMCIYGYSLAIYIPVSILWVINISLLQWLLVITAALLSGTVLIAVLTPALRNSQFSLFLIIGILSAHIVLAAGFMLYFFHNPPDSPVTMPIPTAAPAPSVLKAVTQAAKAVLPGNRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF54</accession>
    <name>A0A6I8UF54_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Tip60</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001354505.4">
        <property type="nucleotide sequence ID" value="XM_001354469.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UF54"/>
    <dbReference type="FunCoup" id="A0A6I8UF54">
        <property type="interactions" value="1835"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814810"/>
    <dbReference type="InParanoid" id="A0A6I8UF54"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079366">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005705">
        <property type="term" value="C:polytene chromosome interband"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046972">
        <property type="term" value="F:histone H4K16 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140861">
        <property type="term" value="P:DNA repair-dependent chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18985">
        <property type="entry name" value="CBD_TIP60_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000022">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000013">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF219">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="17">
        <location>
            <begin position="273"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="204"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="15">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001354505.4"/>
        </source>
    </evidence>
    <sequence length="559" mass="62405" checksum="E56680C3A36674F7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF56</accession>
    <name>A0A6I8UF56_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">C-factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sni</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354461.2">
        <property type="nucleotide sequence ID" value="XM_001354425.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UF56"/>
    <dbReference type="FunCoup" id="A0A6I8UF56">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814376"/>
    <dbReference type="InParanoid" id="A0A6I8UF56"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UF56">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05325">
        <property type="entry name" value="carb_red_sniffer_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF7">
        <property type="entry name" value="NADB-LER2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354461.2"/>
        </source>
    </evidence>
    <sequence length="247" mass="26475" checksum="D917412766AA8651" modified="2020-08-12" version="1">MNSILITGCNRGLGLGLVKALNSLPQPPQHLFTTCRSRAQAVELEDLAKKHSNIHILEIDLKNFDAYEKLIGEIEVVTKGEGLNVLFNNAGVAPKSVRIGATRPQDILDTLHTNTVVPIMLAKACLPLLKKASEANGSEPMGVSRAAIINMSSVLGSIGSNTDGGMYGYRTSKSALNAATKSLSIDLYPQKILCISLHPGWVRTDMGGSSAPLDVPTSTNQIIQTLCKLNEQHNGGFINYDGSQLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG38</accession>
    <name>A0A6I8UG38_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4815016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354950.2">
        <property type="nucleotide sequence ID" value="XM_001354914.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UG38"/>
    <dbReference type="KEGG" id="dpo:4815016"/>
    <dbReference type="InParanoid" id="A0A6I8UG38"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UG38">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032145">
        <property type="entry name" value="DUF4818"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16089">
        <property type="entry name" value="DUF4818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354950.2"/>
        </source>
    </evidence>
    <sequence length="193" mass="21919" checksum="14D6ADCF3AE346BB" modified="2020-08-12" version="1">MVSNLVILATTMLHVGLQASVFVGMDYVLVPGTFFAGWIVYCGLLYFLQDLELLPKHYKRRMGWLSSFVIEVLLSIFIMEVLALFFWSRIVANIYMCIKTAMCYYGGEAEQYYLQHETIIVGTLITSIAAGFSLSATWATDPWESHDEQKETEGKTLQQIADELQEHMDNEKLKKQLEKDLQSADADSLSPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGZ6</accession>
    <name>A0A6I8UGZ6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Single domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4815694</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355340.3">
        <property type="nucleotide sequence ID" value="XM_001355304.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGZ6"/>
    <dbReference type="FunCoup" id="A0A6I8UGZ6">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815694"/>
    <dbReference type="InParanoid" id="A0A6I8UGZ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029277">
        <property type="entry name" value="SVWC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053308">
        <property type="entry name" value="Vago-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957">
        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957:SF1">
        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15430">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01318">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026289076" description="Single domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Single" evidence="4">
        <location>
            <begin position="40"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001355340.3"/>
        </source>
    </evidence>
    <sequence length="112" mass="12002" checksum="36EBFC95FDD6770E" modified="2020-08-12" version="1">MSHFSATLLLCCAVVILGSQCAQADIARADFSNPTYPGKCVLDSNTVMSPGQKGKHPNHPCAGVTCMEGGLVEFKTCDATAPPKGCKMRDFVNTKRSFPECCERSYHCPNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJH8</accession>
    <name>A0A6I8UJH8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mucin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4817251</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356500.4">
        <property type="nucleotide sequence ID" value="XM_001356464.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJH8"/>
    <dbReference type="FunCoup" id="A0A6I8UJH8">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817251"/>
    <dbReference type="InParanoid" id="A0A6I8UJH8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJH8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032086">
        <property type="entry name" value="DUF4813"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38572">
        <property type="entry name" value="BCDNA.GH07269-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38572:SF1">
        <property type="entry name" value="BCDNA.GH07269-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16072">
        <property type="entry name" value="DUF4813"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026025022" evidence="2">
        <location>
            <begin position="30"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="484"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356500.4"/>
        </source>
    </evidence>
    <sequence length="674" mass="66261" checksum="76831A4034332EB1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJI8</accession>
    <name>A0A6I8UJI8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4'-phosphopantetheine phosphatase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Inactive pantothenic acid kinase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4816897</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Phosphatase which shows a preference for 4'-phosphopantetheine and its oxidatively damaged forms (sulfonate or S-sulfonate), providing strong indirect evidence that the phosphatase activity pre-empts damage in the coenzyme A (CoA) pathway. Hydrolyzing excess 4'-phosphopantetheine could constitute a directed overflow mechanism to prevent its oxidation to the S-sulfonate, sulfonate, or other forms. Hydrolyzing 4'-phosphopantetheine sulfonate or S-sulfonate would forestall their conversion to inactive forms of CoA and acyl carrier protein. May play a role in the physiological regulation of CoA intracellular levels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-4'-phospho-S-sulfopantetheine + H2O = (R)-S-sulfopantetheine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:68340"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:177302"/>
            <dbReference type="ChEBI" id="CHEBI:177303"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:68341"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer. Interacts with PKM.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356515.4">
        <property type="nucleotide sequence ID" value="XM_001356479.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJI8"/>
    <dbReference type="FunCoup" id="A0A6I8UJI8">
        <property type="interactions" value="711"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816897"/>
    <dbReference type="InParanoid" id="A0A6I8UJI8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJI8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10880:FF:000001">
        <property type="entry name" value="Pantothenate kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1700.10">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016949">
        <property type="entry name" value="At2g17340"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035073">
        <property type="entry name" value="At2g17340_3_helix_bundle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF35">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030210">
        <property type="entry name" value="UCP030210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0533">Nickel</keyword>
    <keyword evidence="8" id="KW-0944">Nitration</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="13">
        <location>
            <begin position="32"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001356515.4"/>
        </source>
    </evidence>
    <sequence length="363" mass="40540" checksum="CDC4387C9B8A5066" modified="2020-08-12" version="1">MHSCVLLPDAASYHPDTLDLNTDAKAAAYWFPCFRDMVIKFAKQAAKSQAEDSTAEERAEQFKTAYLQQLDDYQSNIANNSGRVILGTSELLKLNETMLRRFGFTDPWRNQKQLENASAKARLKLRLEEIDAIQNPDDKWTELVRGVLAGNMFDWGAQAISNILEQDSSFGLHSALDRIEKRPWLLDNLDNWLKRLRISSDQPPHKCAVVFVDNSGVDVVLGILPFVRELLRRGTKVLLCANSEPSLNDVTSRELSALLDECAGECAVLSSAWSSARLLVYANGQSGPCLDMRTLPRDLCDAIAANETDLLVIEGMGRALHTNLNAHFSCETLKLAVIKNRWLAKYLGGDEMFAVICKYEAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJS5</accession>
    <name>A0A6I8UJS5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4816848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356637.3">
        <property type="nucleotide sequence ID" value="XM_001356601.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJS5"/>
    <dbReference type="KEGG" id="dpo:4816848"/>
    <dbReference type="InParanoid" id="A0A6I8UJS5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027896">
        <property type="entry name" value="UQCC3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15141">
        <property type="entry name" value="UQCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356637.3"/>
        </source>
    </evidence>
    <sequence length="81" mass="9113" checksum="C1EA70EE6CB37421" modified="2020-08-12" version="1">MFGNPYLKSLLWLVSFGGIGYGLMRLTEPNAEKIERIKASARGHPNQTDDEKKKALFMKRLQEAASTSAPIYRTPTGDKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJU8</accession>
    <name>A0A6I8UJU8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tubulin polyglutamylase TTLL4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TTLL4B</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356668.2">
        <property type="nucleotide sequence ID" value="XM_001356632.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJU8"/>
    <dbReference type="FunCoup" id="A0A6I8UJU8">
        <property type="interactions" value="336"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817303"/>
    <dbReference type="InParanoid" id="A0A6I8UJU8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000009">
        <property type="entry name" value="tubulin polyglutamylase TTLL5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF162">
        <property type="entry name" value="TUBULIN MONOGLUTAMYLASE TTLL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="74"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356668.2"/>
        </source>
    </evidence>
    <sequence length="983" mass="109856" checksum="A68925528D0853BA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJZ0</accession>
    <name>A0A6I8UJZ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Pgant4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001356707.4">
        <property type="nucleotide sequence ID" value="XM_001356671.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJZ0"/>
    <dbReference type="FunCoup" id="A0A6I8UJZ0">
        <property type="interactions" value="270"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817245"/>
    <dbReference type="InParanoid" id="A0A6I8UJZ0"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23462">
        <property type="entry name" value="beta-trefoil_Ricin_Pgant9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000029">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF134">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 11" id="KW-0430">Lectin</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="13">
        <location>
            <begin position="526"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="59"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001356707.4"/>
        </source>
    </evidence>
    <sequence length="671" mass="76988" checksum="875F1CB81941C578" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKW2</accession>
    <name>A0A6I8UKW2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein IA-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">IA-2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356992.4">
        <property type="nucleotide sequence ID" value="XM_001356956.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UKW2">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817589"/>
    <dbReference type="InParanoid" id="A0A6I8UKW2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKW2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051046">
        <property type="term" value="P:regulation of secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000017">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase-like N isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2470">
        <property type="entry name" value="Protein-tyrosine phosphatase receptor IA-2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033522">
        <property type="entry name" value="IA-2/IA-2_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021613">
        <property type="entry name" value="Receptor_IA-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038112">
        <property type="entry name" value="Receptor_IA-2_ectodomain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46106">
        <property type="entry name" value="IA-2 PROTEIN TYROSINE PHOSPHATASE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46106:SF4">
        <property type="entry name" value="IA-2 PROTEIN TYROSINE PHOSPHATASE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11548">
        <property type="entry name" value="Receptor_IA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="848"/>
            <end position="871"/>
        </location>
    </feature>
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        <location>
            <begin position="991"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="1170"/>
            <end position="1243"/>
        </location>
    </feature>
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            <begin position="21"/>
            <end position="96"/>
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            <begin position="300"/>
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        <location>
            <begin position="1266"/>
            <end position="1339"/>
        </location>
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        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
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            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
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        <location>
            <begin position="448"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="910"/>
            <end position="929"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="952"/>
            <end position="961"/>
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            <begin position="1266"/>
            <end position="1275"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1289"/>
            <end position="1301"/>
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        <location>
            <begin position="1308"/>
            <end position="1317"/>
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        <location>
            <begin position="1318"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001356992.4"/>
        </source>
    </evidence>
    <sequence length="1339" mass="148515" checksum="3C569EACFFA55C26" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL13</accession>
    <name>A0A6I8UL13_DROPS</name>
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        <submittedName>
            <fullName evidence="17">Zinc finger protein 70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC4817742</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:sequence-specific DNA binding"/>
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    <dbReference type="GO" id="GO:0008270">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
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        </location>
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            <begin position="212"/>
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        </location>
    </feature>
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    </feature>
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            <end position="367"/>
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            <end position="420"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001357142.3"/>
        </source>
    </evidence>
    <sequence length="584" mass="66692" checksum="6A15B53B55DE59CA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULR3</accession>
    <name>A0A6I8ULR3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4818168</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_001357373.4"/>
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    </dbReference>
    <dbReference type="KEGG" id="dpo:4818168"/>
    <dbReference type="InParanoid" id="A0A6I8ULR3"/>
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    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357409.3"/>
        </source>
    </evidence>
    <sequence length="254" mass="29953" checksum="888349138317E22C" modified="2020-08-12" version="1">MKKPLGNGEGREGGLGEEGEEEEEPHLTPQLQDHYETEAEVLGLLVDLETRYRDFYNMYLCEVQAQKILIERVWLLTQRYLILISCVIGCRYPEVYTLSTEEALVNEYEEKLEVLRTSNNNMKRAVLELDTHCKRFYAAYDRLNKSLETPLIMGDPSHRCIQHHKIMAVDIFNYFHATVLKLKCYMHQLDPLNLESVEEYRELLKTEAQCEEFEGHLMENFVYCKCLHKRPTCPVEKLKCYHHNIANLKYVSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM16</accession>
    <name>A0A6I8UM16_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Ibf1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Ibf1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357558.3">
        <property type="nucleotide sequence ID" value="XM_001357521.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM16"/>
    <dbReference type="FunCoup" id="A0A6I8UM16">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="GeneID" id="4800357"/>
    <dbReference type="KEGG" id="dpo:4800357"/>
    <dbReference type="InParanoid" id="A0A6I8UM16"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081065">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357558.3"/>
        </source>
    </evidence>
    <sequence length="242" mass="27668" checksum="2EAE4FFD08D1C59B" modified="2020-08-12" version="1">MPRKKSEDFYKKFGFSAKCENGKYTATCTFCNKELQNTALTRLSLHRQTCDQKRALSETQSINMERARPVLKIHKITHEDADGGIDHGNEGKEIIVKIQEFLDECDDDTDGKEQATTVKSELMNSEQNSPHLNASKEEEAMEIEITAGPESREYVEFLDEYKEVIQSLDDPANGTDTNFGENRPKNNLALLKADKARAEIKQYNSKAMFLRTETENLKLERTLTLLKIQKLRLEIDCLRAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMT2</accession>
    <name>A0A6I8UMT2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA-binding protein Ikaros</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Boh1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357914.1">
        <property type="nucleotide sequence ID" value="XM_001357877.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMT2"/>
    <dbReference type="FunCoup" id="A0A6I8UMT2">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="4800688"/>
    <dbReference type="KEGG" id="dpo:4800688"/>
    <dbReference type="InParanoid" id="A0A6I8UMT2"/>
    <dbReference type="OMA" id="CNAMSHS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077386">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357914.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="38608" checksum="020E437B94641D67" modified="2020-08-12" version="1">MANALRPIPAKVKPINVRVFELPEFIFVQTKLITPRYALENRILTTRLKKPWARTKSAILAPAAKVERPPPKIVPLPSRTGPQKTPETSYPVRERAIQTEEKQTCDVGVQCDTEEWEETQMDTERKGELSSGEEDEESKFITFVGENTLIIRNGMMECLVCGEVARSLEHHKSHIVTHYGPKVLCCLCGEFVDHERLLLGHNLTCRSRPSKKPSIFLKCPNMFCNVVCRTQPQLYKHLSTHDKGPFYRCLECRLHFRTAMSFLLHRKKKEDCNRAKALFLFDKHSASRLKGNLKRCTVCLRKFNDERLCGIHRFNCIQAHHKKLTKKLFKPSSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMU1</accession>
    <name>A0A6I8UMU1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">28S ribosomal protein S22, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mRpS22</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357913.2">
        <property type="nucleotide sequence ID" value="XM_001357876.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMU1"/>
    <dbReference type="FunCoup" id="A0A6I8UMU1">
        <property type="interactions" value="811"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800687"/>
    <dbReference type="InParanoid" id="A0A6I8UMU1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019374">
        <property type="entry name" value="Ribosomal_mS22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13071">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13071:SF4">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10245">
        <property type="entry name" value="MRP-S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001357913.2"/>
        </source>
    </evidence>
    <sequence length="393" mass="46075" checksum="07C842677A99D4DF" modified="2020-08-12" version="1">MSLLRYVRRLNALQGHQHCRREVSSAPAKSATLKYDQDPQPLFTDSETQRLLQSMTQLQLDKVFRKRTVKDNRSETKFMTNEQLETEFLQIIEKAKHLLKMPPIVKIKQDTERIIAKDPALKDFATTKYVFTDITFGLPHSERKVVVRETDGTLAYASLDTTKRMNQLYFPLEGRKSYTPRMFAYEELLHKCLEEHKYEFVLDRLTVQYEPYETEFHNLSARVFEHLNESKQFDLLRSTRHFGPMAFFYAWHRCIDDLLYDMIRRDYLHNAVELIALTYKIHKIPVEYTETLAKLEALHPTPDVSALSELRGFLRRPEEKQGIEQEIQTAIGKTEQDFAADDISLKFIEEYIASEHSLKKVQLELAVQTLKEINLEKLQLFQGLKKAHGVQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN18</accession>
    <name>A0A6I8UN18_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4800969</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358151.2">
        <property type="nucleotide sequence ID" value="XM_001358114.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN18"/>
    <dbReference type="FunCoup" id="A0A6I8UN18">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800969"/>
    <dbReference type="InParanoid" id="A0A6I8UN18"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358151.2"/>
        </source>
    </evidence>
    <sequence length="397" mass="45799" checksum="30485DE17D8FE730" modified="2020-08-12" version="1">MFERLREVRLSGLSAYILRFSYHYARVLGVICFEVSKKGPKDALVGRKNFRLKWYSLILRLVCFAVVCCFCAPLVMTIEDPNERTLQCMRLVSCLVCTACILVLQIFHEHEVLRMINSFLRVFRRVRQMSTRRKYGFGGKREFVLLLFKAICLTYELFCELHQILFASSLVVTLIALCDIIVEIGSLMIIHIGFAGFLSVAALYSEVNGYVRIELRRQLRSLERPGGAPASRRQLRTASCRLEECVAVYDEIERVGRSFHILLQLPVLIILVAKIFATTVLSYEMIIRVDRYPCQAGIWGLVLKSFADVILLTLAVHEAVSSSKVIRRLSLENFPVSDQKEWHVKLEMFLSRLNFNEFRVCPLGLFEVSNEIILVFLSSMITYFTYVMQYGIQTHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN32</accession>
    <name>A0A6I8UN32_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-terminal kinase-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">SCY1-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">yata</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. Involved in the maintenance of the Golgi apparatus morphology.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358173.3">
        <property type="nucleotide sequence ID" value="XM_001358136.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN32"/>
    <dbReference type="FunCoup" id="A0A6I8UN32">
        <property type="interactions" value="2668"/>
    </dbReference>
    <dbReference type="GeneID" id="4800998"/>
    <dbReference type="KEGG" id="dpo:4800998"/>
    <dbReference type="InParanoid" id="A0A6I8UN32"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075190">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF3">
        <property type="entry name" value="N-TERMINAL KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="16"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="583"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="691"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="765"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="610"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="633"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="655"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="691"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="720"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="765"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="783"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="799"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="839"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00042347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358173.3"/>
        </source>
    </evidence>
    <sequence length="877" mass="97714" checksum="0409BF74E1C56025" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UND9</accession>
    <name>A0A6I8UND9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Baculoviral IAP repeat-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Det</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358213.4">
        <property type="nucleotide sequence ID" value="XM_001358176.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UND9"/>
    <dbReference type="FunCoup" id="A0A6I8UND9">
        <property type="interactions" value="574"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801050"/>
    <dbReference type="InParanoid" id="A0A6I8UND9"/>
    <dbReference type="OMA" id="HAPQCEF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071570">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000009">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF5">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001358213.4"/>
        </source>
    </evidence>
    <sequence length="152" mass="17247" checksum="CF8020F60C6D7E41" modified="2020-08-12" version="1">MDATAATDATGAVVGDKLEMFRQLNLLEQHRVESFKDWPFPEQSSCSISKMAEAGFYWTGTKRENDTATCFVCNKTLDGWESDDDPWKEHLKHAPQCEFVKLGCAEKDLTVTQFLEILGRVVKTSIEKNCKAFKQGFISDNENSLDKFTHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNY8</accession>
    <name>A0A6I8UNY8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alpha-2-macroglobulin receptor-associated protein isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Alpha-2-macroglobulin receptor-associated protein isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">LOC4801500</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <strain evidence="5">MV2-25</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358579.3">
        <property type="nucleotide sequence ID" value="XM_001358542.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236716.1">
        <property type="nucleotide sequence ID" value="XM_033380825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNY8"/>
    <dbReference type="KEGG" id="dpo:4801500"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050750">
        <property type="term" value="F:low-density lipoprotein particle receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048259">
        <property type="term" value="P:regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14808">
        <property type="entry name" value="RAP_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.10">
        <property type="entry name" value="RAP domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038003">
        <property type="entry name" value="A2-macroglobuin_RAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010483">
        <property type="entry name" value="Alpha_2_MRAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009066">
        <property type="entry name" value="MG_RAP_rcpt_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037999">
        <property type="entry name" value="RAP_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036744">
        <property type="entry name" value="RAP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16560">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16560:SF2">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06401">
        <property type="entry name" value="Alpha-2-MRAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06400">
        <property type="entry name" value="Alpha-2-MRAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47045">
        <property type="entry name" value="RAP domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634450" evidence="2">
        <location>
            <begin position="23"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin receptor-associated protein" evidence="3">
        <location>
            <begin position="14"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin RAP C-terminal" evidence="4">
        <location>
            <begin position="158"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="340"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358579.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033236716.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="44745" checksum="0A2F8CF8CA59E6D6" modified="2020-08-12" version="1">MQSFGQLGLVLALALLITCVDADKKQSKKYSREANDPHFQQVADEKYDPDFKKIRRPFRMAKLNLVWSKAQNRLTEPKLKSLYMELKIHDKEEIAWKQLNSQHKDKDGLKENELRRKLIGIMSSYDLLEHFDETEDAEKIKPFKKFHDPEERHKNKSLFKDKKLNRLWEKAEVSGFTSEELKSLKQEFEHHQDKVDVYYSLLENIGSVDTDKHENAINTEDLDTYNLISNNDANENEIKTHDQNVKKFENDLNTLRGHHVGIKDHYDRLERLVSSGPHSQDFIEPKVQGLWRVAQASNFTVKELASIKTELNHFESRLLKLRHLHAEHALHKEKYKDEKHKDKSNRFEDMEDQLKKQARKVEKLQENIEKTIFKHSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP05</accession>
    <name>A0A6I8UP05_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">CLIP domain-containing serine protease 14D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4801518</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358596.5">
        <property type="nucleotide sequence ID" value="XM_001358559.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP05"/>
    <dbReference type="KEGG" id="dpo:4801518"/>
    <dbReference type="InParanoid" id="A0A6I8UP05"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000028">
        <property type="entry name" value="Serine protease easter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038565">
        <property type="entry name" value="CLIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026361766" evidence="10">
        <location>
            <begin position="22"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="245"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001358596.5"/>
        </source>
    </evidence>
    <sequence length="509" mass="55494" checksum="84EF55A2AE9FBA8A" modified="2020-08-12" version="1">MRQQLAKLVCLLCCTLCLLRSDFGCASLRACNCIRIKECVAFTRLLLHHQPEERAAVFAKVHAAGCGFEGIDPLVCCPQSQHQSRHRHESTVRPMRMLRFAPPDERWIWGDSRLSPPAFGQDETEMNLHDYWDFKEQRNCPQPVDPEFYDHRFGSGSGHSFHYDIDHGSNDWHSPRPAPEDKPIVFPGDLRFLRHGGEQETSTDVSQGPPLAPFTTALPSTTTSASPTAVPATVSSCGISIESRLLGGEQATAGQYPWLARIAYRNRSSNRISFRCSGSLIASNYVVTAAHCVVNLVGDLEVSHVRLGDAPANAEDASNLFAIDQVLVHPNFDQPRYANDIALLRLNSTASTFTPICLPLNSTETLGNRLIGQTGVAAGWSTSNSEGNISTNSTSSTVRFIRLPIVNTTSCAIAYATLSENFQQPIVITPSHLCAQGQPMNDVCRGDSGGPFMDDGTSGLLAINGRYTLIGIVAFGPTLCGVTTIPGVYTLVSSFSDWIQRSINRTPNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPB3</accession>
    <name>A0A6I8UPB3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB/POZ domain-containing protein KCTD3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4801497</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KCTD3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358576.3">
        <property type="nucleotide sequence ID" value="XM_001358539.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPB3"/>
    <dbReference type="FunCoup" id="A0A6I8UPB3">
        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801497"/>
    <dbReference type="InParanoid" id="A0A6I8UPB3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18363">
        <property type="entry name" value="BTB_POZ_KCTD3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000038">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047876">
        <property type="entry name" value="SHKBP1/KCTD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15859:SF1">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15859">
        <property type="entry name" value="SETA BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="11"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="143"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358576.3"/>
        </source>
    </evidence>
    <sequence length="751" mass="81942" checksum="0A892FC79BD367BA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPM0</accession>
    <name>A0A6I8UPM0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCT-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CCT1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358710.1">
        <property type="nucleotide sequence ID" value="XM_001358673.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPM0"/>
    <dbReference type="SMR" id="A0A6I8UPM0"/>
    <dbReference type="FunCoup" id="A0A6I8UPM0">
        <property type="interactions" value="2178"/>
    </dbReference>
    <dbReference type="GeneID" id="4801658"/>
    <dbReference type="KEGG" id="dpo:4801658"/>
    <dbReference type="InParanoid" id="A0A6I8UPM0"/>
    <dbReference type="OMA" id="RGPNDYQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078830">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03335">
        <property type="entry name" value="TCP1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000009">
        <property type="entry name" value="T-complex protein 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000045">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012715">
        <property type="entry name" value="Chap_CCT_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02340">
        <property type="entry name" value="chap_CCT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001358710.1"/>
        </source>
    </evidence>
    <sequence length="557" mass="59588" checksum="187A644B131A4A43" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPS1</accession>
    <name>A0A6I8UPS1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Osi1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Osi1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358928.5">
        <property type="nucleotide sequence ID" value="XM_001358891.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPS1"/>
    <dbReference type="FunCoup" id="A0A6I8UPS1">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801909"/>
    <dbReference type="InParanoid" id="A0A6I8UPS1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012464">
        <property type="entry name" value="DUF1676"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879">
        <property type="entry name" value="FI03362P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21879:SF26">
        <property type="entry name" value="OSIRIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07898">
        <property type="entry name" value="DUF1676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358928.5"/>
        </source>
    </evidence>
    <sequence length="420" mass="46564" checksum="BB78DB9FAC2FF911" modified="2020-08-12" version="1">MRSIQFAVRSLIVQQKLAPRSESDPESPVALALALHPQLGGRNCDSGACCMHMSIRRADEVQLAKQLSSLRASACGCLAVIKGVASAFWLLFVSRPVSRAVSQLSQTRLAFQIDIKMLLCRIVVALMLLLLLHYGSAEEQATDAPQSTPAIHRSNGRAGSSLLSHSDAYIARNQRQCFDTRNLMSCIKYKASKLIWKLATNGMGFFPNEYGRDLAADKHRLLRLVQLGEPADDIVVFNDAKSLEGDSELTLIFKFLKRAVETFGRNHGLQLALSSETGARVMDESEARLKKKKRKWLIILPLVILMKIAHLKMTLVSMLLTVIGMNVLLVGGVGWLIHYLKYKTMCKIHPHLVQTHSHVYDSEPSDYSQFVGGSSFSNSYSPYSSGAGAGHDGNNYSKDWATNKAYNGYNYLDTISKRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ90</accession>
    <name>A0A6I8UQ90_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4801979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358991.4">
        <property type="nucleotide sequence ID" value="XM_001358954.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ90"/>
    <dbReference type="FunCoup" id="A0A6I8UQ90">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801979"/>
    <dbReference type="InParanoid" id="A0A6I8UQ90"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031974">
        <property type="entry name" value="PDCD7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16021">
        <property type="entry name" value="PDCD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358991.4"/>
        </source>
    </evidence>
    <sequence length="240" mass="27455" checksum="D3D2940E9AAF31D2" modified="2020-08-12" version="1">MPAKNPNLYEIKTNTEQSAADLQKLDVASNAPPRRVDAGQLEAPQETRIAQLDEIDSRTAAKIAGIQRRRKRRRWKFRSKQSAKRVLENEHVPKPPDEPRPSPMDKKTEGITLKKRSDAASVLERLDLLVKLCEARGGDTATLVEKLASMRTVWQRVQQESTSGTINKSKSPLIETQWDVVFFGKPSSCQKRRLNKPEFLKRRSIWDSYITHGKRGSCIPRGWVLPTQEPTSEWVEYRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR14</accession>
    <name>A0A6I8UR14_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pachytene checkpoint protein 2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pch2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. PCH2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359525.3">
        <property type="nucleotide sequence ID" value="XM_001359488.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR14"/>
    <dbReference type="FunCoup" id="A0A6I8UR14">
        <property type="interactions" value="1551"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802644"/>
    <dbReference type="InParanoid" id="A0A6I8UR14"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19508">
        <property type="entry name" value="RecA-like_Pch2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001494">
        <property type="entry name" value="Pachytene checkpoint component Pch2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044539">
        <property type="entry name" value="Pch2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058249">
        <property type="entry name" value="Pch2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991:SF1">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23242">
        <property type="entry name" value="AAA_lid_TRIP13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="159"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359525.3"/>
        </source>
    </evidence>
    <sequence length="418" mass="45891" checksum="8A6F6B3E7BCCD325" modified="2020-08-12" version="1">MSATHVEVRLNSTDQPIPALRFVLTPAVNAFLKSNNNLPHNVAFKPDLVETDPSLVASVLLERDCGGGPEIAGETKAFFHFYKPHAAAATADSGLFDSAVDADGAAVVDANHVLLPSKKFVGLWETLIFEKGLKERLLKFAMSALTFSKHHVDTNLIACNRLILLHGPPGTGKTSLCKALAQKLAIRTHEAFAYTHLVEINSHSLFSKWFSESGKLVARLFAKIGELVADRNNLVCLLIDEVESLAYARSSMNSNEPRDAMRVVNALLTHLDEIKTHPNVLILATSNLAQSIDLAFLDRADLREYIGYPAVPAIRSIYKSMLVELMSAGIVECEALETEDIEEGLLTNLSERSVGLSGRTLRKLPLLAHAQYTASELFEENGKIFLSDFLDAMLLALEQNIRQHQLLKSESMEGPNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US19</accession>
    <name>A0A6I8US19_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Oligomycin sensitivity conferral protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ATPsynO</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359895.3">
        <property type="nucleotide sequence ID" value="XM_001359858.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US19"/>
    <dbReference type="FunCoup" id="A0A6I8US19">
        <property type="interactions" value="1584"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803097"/>
    <dbReference type="InParanoid" id="A0A6I8US19"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.20:FF:000002">
        <property type="entry name" value="ATP synthase subunit O, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="ATP SYNTHASE DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359895.3"/>
        </source>
    </evidence>
    <sequence length="210" mass="22541" checksum="1E70CA8385B79FDC" modified="2020-08-12" version="1">MASINKLAIVSRALATAAASTKSVKPPLQLFGLEARYATALYSAATKLNTLDQVEKDLIALQTSLKTDKKLRENVTSPIINRKVMAIALREASGKLNFAPATANILGLLADNGRLKKLDNVITAFRTIMAAHRGEVVCEVVSAKPLDSSQSKQLEGALKSFLKDNQSLKITSRVDPSIIGGLIVSIGDKYVDMSIATKVKLYTDVMQTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US31</accession>
    <name>A0A6I8US31_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4803224</name>
        <name evidence="9" type="synonym">LOC117183968</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <strain evidence="7 8">MV2-25</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360000.1">
        <property type="nucleotide sequence ID" value="XM_001359963.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235612.1">
        <property type="nucleotide sequence ID" value="XM_033379721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US31"/>
    <dbReference type="SMR" id="A0A6I8US31"/>
    <dbReference type="GeneID" id="4803224"/>
    <dbReference type="KEGG" id="dpo:117183968"/>
    <dbReference type="KEGG" id="dpo:4803224"/>
    <dbReference type="OMA" id="IHDSYSM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076241">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009244">
        <property type="entry name" value="Mediatior_Med7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044888">
        <property type="entry name" value="Mediatior_Med7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05983">
        <property type="entry name" value="Med7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001360000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033235612.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="25851" checksum="49A2C47025C0B640" modified="2020-08-12" version="1">MSTQETQVSSLPMPPERYISNYTEENIRRNRAPRPPLPPSQHEVYSMFGIQYNNDEMIRSLESQNIKRLIPIHFDRRKELKKLNHSLLVNFLDLIDFLILNPDSPRRTEKIDDLSLLFVNMHHLLNEFRPHQARETLRVMMEMQKRQRVETATRFQKHLERVRDIVNSAFSALPVLCDDDEKGNAKVKSEVDPLESNAAAKNDPSYQHDRMMCKLVDGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USG7</accession>
    <name>A0A6I8USG7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein 60A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein glass bottom boat</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gbb</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360218.3">
        <property type="nucleotide sequence ID" value="XM_001360181.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USG7"/>
    <dbReference type="FunCoup" id="A0A6I8USG7">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803504"/>
    <dbReference type="InParanoid" id="A0A6I8USG7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13761">
        <property type="entry name" value="TGF_beta_BMP5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000003">
        <property type="entry name" value="Bone morphogenetic protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000030">
        <property type="entry name" value="Glass bottom boat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF310">
        <property type="entry name" value="PROTEIN 60A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="13">
        <location>
            <begin position="336"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="323"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001360218.3"/>
        </source>
    </evidence>
    <sequence length="459" mass="52141" checksum="087B358A18C57A36" modified="2020-08-12" version="1">MSIMFSSIRNTSEAVAALASLGLGFVLIIFVSTTPTVIDATQSGIYIDNGKDQTIMHRVLSDDDKLDVSYEILEFLGIAERPTHHSSHQLSLRKSAPKFLLDVYHRITEEEGLGGGHTDDETVHSHRSKRNADMSDERNADGEENFITDLDKRAIDESDIIMTFLNKRNHNVEELRHEHGRRLWFDVSKVPSDNYLVMAELRIYQNSNEGKWLTANKQFTITVYSMGLGESGQNTMEALSSVNTTGDYVGWLELNVTEGLHEWLVHSKENHGIYIGAHAVNRPDREVKLDDVGLIHRKVDDEYQPFMIGFFRGPELIKTTSHSSHHRSKRSTLHQRKKKKSVPVHPFLDVPIENTRSCQMQTLYIDFKDLGWHDWIIAPEGYGAFYCSGECNFPLNAHMNATNHAIVQTLVHLLEPKKVPKPCCAPTRLGALPVLYHLNDENVNLKKYRNMIVKSCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTU9</accession>
    <name>A0A6I8UTU9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4804272</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360854.3">
        <property type="nucleotide sequence ID" value="XM_001360817.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTU9"/>
    <dbReference type="KEGG" id="dpo:4804272"/>
    <dbReference type="InParanoid" id="A0A6I8UTU9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032222">
        <property type="term" value="P:regulation of synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031424">
        <property type="entry name" value="QVR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050975">
        <property type="entry name" value="Sleep_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562">
        <property type="entry name" value="ATILLA, ISOFORM B-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562:SF29">
        <property type="entry name" value="PROTEIN SLEEPLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17064">
        <property type="entry name" value="QVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026026123" evidence="9">
        <location>
            <begin position="25"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001360854.3"/>
        </source>
    </evidence>
    <sequence length="161" mass="17971" checksum="5F742E3040AC07B2" modified="2020-08-12" version="1">MGIGTKLWPTVGTVVFLIGSVAVGEILKCYQCNEADQTCGGPQKPLGHIGECPHSTMCTKTVLTMSLLEGNQWIKTMRGCAQQLSTTHVFVNKSWEEKSVVEEPYEEGCIEEKVNFRNTVLLCHCRGDLCNASSSPALDGRMLPLILIGFIWYWSYRKLHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV02</accession>
    <name>A0A6I8UV02_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361034.2">
        <property type="nucleotide sequence ID" value="XM_001360997.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV02"/>
    <dbReference type="GeneID" id="4804476"/>
    <dbReference type="KEGG" id="dpo:4804476"/>
    <dbReference type="InParanoid" id="A0A6I8UV02"/>
    <dbReference type="OMA" id="MVYPCYC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072406">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026353024" evidence="1">
        <location>
            <begin position="19"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361034.2"/>
        </source>
    </evidence>
    <sequence length="100" mass="12021" checksum="87F21AA7FBC117A1" modified="2020-08-12" version="1">MLFIRLILFCSLTAIVLANIQRTAARHKPLSWWQKQYYQSKWHQRFRFTTTAVPGATPESKEVRMVYPCYCYRPPQEGEVTTAIYERYILEREDLFILNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVI0</accession>
    <name>A0A6I8UVI0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Serine protease 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Jon44E</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361522.1">
        <property type="nucleotide sequence ID" value="XM_001361485.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVI0"/>
    <dbReference type="FunCoup" id="A0A6I8UVI0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="GeneID" id="4805058"/>
    <dbReference type="KEGG" id="dpo:4805058"/>
    <dbReference type="InParanoid" id="A0A6I8UVI0"/>
    <dbReference type="OMA" id="WGHTSND"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081163">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UVI0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000164">
        <property type="entry name" value="Jonah 66Cii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8 12" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026081083" evidence="9">
        <location>
            <begin position="19"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="43"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361522.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="30075" checksum="26B86D5812087C94" modified="2020-08-12" version="1">MQLFVFLALALAVAVASAVPTEVANKPVPVRDMPSAAKIQGRITNGYPAYEGKVPYIVGLSFNDNYWCGGSIIGHNWILTAAHCTREANHVLIYYGASFRHEAQFTHWVSRDDIISHPDFNDHLNNDIGLIRTPHVDFWSLVNRVELPSYNDRYNSFAGWWAVASGWGHTSNDSGMSNYLNCVDVQLMDNNDCRNVYGDSYITDNTICIQTHGAQSTCNGDSGGPLVLHEGNRIVGITSFGHWNGCTAGEPAGFTRVTGYLDWIRDHTGISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW05</accession>
    <name>A0A6I8UW05_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FAS-associated factor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">casp</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361766.2">
        <property type="nucleotide sequence ID" value="XM_001361729.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW05"/>
    <dbReference type="FunCoup" id="A0A6I8UW05">
        <property type="interactions" value="2030"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805352"/>
    <dbReference type="InParanoid" id="A0A6I8UW05"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UW05">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14413">
        <property type="entry name" value="UBA_FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01771">
        <property type="entry name" value="UBX_UBXN3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033043">
        <property type="entry name" value="FAF1-like_UBX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049483">
        <property type="entry name" value="FAF1_2-like_UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044541">
        <property type="entry name" value="FAF1_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF96">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21021">
        <property type="entry name" value="FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="624"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361766.2"/>
        </source>
    </evidence>
    <sequence length="704" mass="79541" checksum="DECF0819DB73BD84" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWB3</accession>
    <name>A0A6I8UWB3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein Ih isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">Ih</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361884.4">
        <property type="nucleotide sequence ID" value="XM_001361847.4"/>
    </dbReference>
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        <property type="term" value="P:regulation of membrane depolarization"/>
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    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001361884.4"/>
        </source>
    </evidence>
    <sequence length="1311" mass="146768" checksum="D3F9C3F15BE52EE0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWJ3</accession>
    <name>A0A6I8UWJ3_DROPS</name>
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        <submittedName>
            <fullName evidence="9">Death-associated inhibitor of apoptosis 2</fullName>
        </submittedName>
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        <name evidence="9" type="primary">Diap2</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
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        </source>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
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    </dbReference>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="BIR_rpt"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044:SF139">
        <property type="entry name" value="DEATH-ASSOCIATED INHIBITOR OF APOPTOSIS 2"/>
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    </dbReference>
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        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
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    </dbReference>
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        <property type="entry name" value="zf-C3HC4_3"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01282">
        <property type="entry name" value="BIR_REPEAT_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="446"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361948.2"/>
        </source>
    </evidence>
    <sequence length="493" mass="54425" checksum="9A2A0D86D1536EB7" modified="2020-08-12" version="1">MTELRLELESVRTATFVQWPLNAPVSAEDLVTNGFFATGNWLEAECNWCHVRIDRWEYGDQVAERHRRCSPICSMVLAPSHCGNITLSQENDIEGNTMVDGGGTASPCVCPDLHLEANRLETFKDWPNPNVTPQALAKAGFYYLNRLDHVKCVWCNGVIAKWEKNDNAFDEHRRFFPNCPRVQMGPLIEFAAGKNLEELGIQPTTQPKLPNFACVDSRLRTFTDWPIGNIQPPEPLAQAGLFYQKIADQVRCFHCNIGLRSWQKEDEPWHEHAKWSPKCQFVLLAKGPAFVREVGEAMAASSGSQIATAPALQLNALMDESPAKEALALGINGGVLRNTIQRKLSSSGSAFETLDELLHAIFDEAGCETALEVREPTEPTAPPLDCCQATTSKTAVSSDSTQNKAEPSVVPEANISEQMQRMSVAPPNGNISLEEENRQLKDARLCKVCLDEEVGVVFLPCGHLATCNQCAPSVANCPLCRADIKGFVRTFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWU9</accession>
    <name>A0A6I8UWU9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GDP-fucose protein O-fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6903365</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Protein O-fucosyltransferase that specifically catalyzes O-fucosylation of serine or threonine residues in EMI domains of target proteins. Attaches fucose through an O-glycosidic linkage. O-fucosylation of EMI domain-containing proteins may be required for facilitating protein folding and secretion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_002132247.3">
        <property type="nucleotide sequence ID" value="XM_002132211.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWU9"/>
    <dbReference type="FunCoup" id="A0A6I8UWU9">
        <property type="interactions" value="580"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903365"/>
    <dbReference type="InParanoid" id="A0A6I8UWU9"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UWU9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017176">
        <property type="entry name" value="Alpha-1_3-FUT_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF194">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037332">
        <property type="entry name" value="Alpha1_3FUT_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="14">
        <location>
            <begin position="44"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="13">
        <location>
            <begin position="174"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002132247.3"/>
        </source>
    </evidence>
    <sequence length="442" mass="51723" checksum="79F4ABCEA65FFB38" modified="2020-08-12" version="1">MRFAHRWIIGITCLILVWLLHFVCWDLVELFDVQKSTTAAPIELLWWSHHFSFVYDEVRQCGAFECRVTNKRQRLSSARAVLFYGTALKTGDFPMPRSPQHLWALLHEESPRNVIYAPFDDFLQHFNFTSTFSRYSDQPITTQWLPSAHDLISMDYTMSFDFKSSNLNGPSPSVVFLNSDCDTMSGREDYMKELMRHINVDSFGSCLRNKDLPASLQNDYLNNLYSPKLLRFLSRYKFMIAIENAVCEDYITEKFWRPLVVGVIPIYFGSPSIKDWQPQKKSAIFVSDFPNAAALGRYIMELSENKTEYDSFRGHKLNRSHPIQNEKLLHHLITYPHQTREYDFFETFECAVCKYATHSGPIKRANARHYNCPLVPIYAPLESKKEPERATDWRSAMEVGQCQARILDTFFRSNKPYNEADFEAELNRRMDDNRCSIDPKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXH1</accession>
    <name>A0A6I8UXH1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">LOC6902879</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10">MV-25-SWS-2005</strain>
            <tissue evidence="9 10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a role in muscle cell metabolism.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132613.2">
        <property type="nucleotide sequence ID" value="XM_002132577.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235822.1">
        <property type="nucleotide sequence ID" value="XM_033379931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXH1"/>
    <dbReference type="KEGG" id="dpo:6902879"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXH1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000020">
        <property type="entry name" value="Cytochrome b5-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053100">
        <property type="entry name" value="Cytochrome_b5-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16740">
        <property type="entry name" value="CYTOCHROME B5-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16740:SF1">
        <property type="entry name" value="CYTOCHROME B5-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="7">
        <location>
            <begin position="37"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00055674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002132613.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033235822.1"/>
        </source>
    </evidence>
    <sequence length="440" mass="51449" checksum="8ACA6C7CF6BF8771" modified="2020-08-12" version="1">MTPNMEEEAWRVSGISKNYPSYRQHQPATVESWLEGKHSDDGAEGLWRINDKLYDLSDFATRHPGGAYWIECTRGTDITEPFESHHIEDRARQLLSKFEVREAAQPRNYKFTFDQNGFYMTLKRRVREKLRKMNYSPTKKTDYLHSGILASVFMLSWIGTVLNSLFVLSIAGLALCWLANAAHNYFHQRDNWRMYTFNLTLMSFRNWRISHALSHHVYPNSLHDLELSMFEPFLCWVPNPHYGSRIKRWISVVVSPLYYVLSFSMAFLQRLVISLAKKNIMYWDDLIAFTLPLFLYLSTGVGLSAALWNWLHVLAVGAFFFGFIGLTAAHHDPRILHDGDAQRTDRDWGLYQMDTVIDRRDVKWSDLLVLTHFGDHALHHLFPTLDHGVLKHLYPELNQTIQEFDAELREMNHWGHIKGQTQQLLRMEANPMPPGSKKFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXH9</accession>
    <name>A0A6I8UXH9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine/threonine-protein kinase polo-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6902946</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132672.2">
        <property type="nucleotide sequence ID" value="XM_002132636.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXH9"/>
    <dbReference type="KEGG" id="dpo:6902946"/>
    <dbReference type="InParanoid" id="A0A6I8UXH9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF93">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="26"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002132672.2"/>
        </source>
    </evidence>
    <sequence length="385" mass="43930" checksum="33BD22237C65B28C" modified="2020-08-12" version="1">MTAAPENKTTYVVPDSLFDPHRGRTYKRGRFFGKGGFARCYEIIDVHTDDVFACKIVSKKLMMKPRQEEETAEPEEIAVEISIHRGLNHRSIVKFFSCFDDIDNTYIVLELCRKRSMSELQRRRKIITEFECRYYITQIIEGVQYLHNNRIIHRDLKLANLFLNDMLHVKIGDFGLATRIEFEGDRRYSVCGSPNYIAPEILTKKGHSFEADIWSIGCVMYTLLAGRPPFHHKTREGIFSNIKKGQYSVPSWLTISAADMLVSMLQLDPERRPAIGQLLNCNFLKAAKVPQFLPSFCLTMAPRIGNGMGISDPVKESMQRRPLMVLNGLRDDNRNDASAQAQAQALAQAQVCRYNDSFDTLIENQGEGNTGQEFVSNNTLCPRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYI2</accession>
    <name>A0A6I8UYI2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch repeat and BTB domain-containing protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133318.3">
        <property type="nucleotide sequence ID" value="XM_002133282.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYI2"/>
    <dbReference type="KEGG" id="dpo:6902372"/>
    <dbReference type="InParanoid" id="A0A6I8UYI2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UYI2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22667">
        <property type="entry name" value="AT01380P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22667:SF0">
        <property type="entry name" value="AT01380P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BACK" evidence="2">
        <location>
            <begin position="151"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133318.3"/>
        </source>
    </evidence>
    <sequence length="313" mass="36025" checksum="DFCF36E2552EECAB" modified="2020-08-12" version="1">MDVRDDKESISVTEMKTTEEPGQTPKWLPFPRNVRTFLLPLQVLCKNPVLVSVEDKVFKCSKVVLVANCQIFSSTLEIGHTLKLTASCLSSKRFIFAYGWMVTNHAAIPYEEMINMLMVADFLKCSPLEKSILTIFNDVDGYLVKDSVRFYFEVIEKGEKGDRLAREMLPRVGHSFLILVASKEFAKVTAKQLYDLLSSSHLAVQSESEVLWAVICWLREDYENRQEHGGYLFKQLQNKRIWIKDPLCPYLNRTSKLSMKMFRDYLQLLNAEPEANFLSRIEILSPEIPPAVPQENILDKILENIYENILSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYT6</accession>
    <name>A0A6I8UYT6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein be</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">be</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133732.2">
        <property type="nucleotide sequence ID" value="XM_002133696.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYT6"/>
    <dbReference type="KEGG" id="dpo:6902205"/>
    <dbReference type="InParanoid" id="A0A6I8UYT6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UYT6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026241691" evidence="3">
        <location>
            <begin position="25"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Right handed beta helix" evidence="4">
        <location>
            <begin position="264"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="754"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="804"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="870"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="613"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="754"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="821"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002133732.2"/>
        </source>
    </evidence>
    <sequence length="892" mass="98080" checksum="EB0A1002C0E860C1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ73</accession>
    <name>A0A6I8UZ73_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Smad nuclear-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC6901176</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for pre-mRNA splicing as component of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002133877.2">
        <property type="nucleotide sequence ID" value="XM_002133841.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ73"/>
    <dbReference type="FunCoup" id="A0A6I8UZ73">
        <property type="interactions" value="460"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901176"/>
    <dbReference type="InParanoid" id="A0A6I8UZ73"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZ73">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22718">
        <property type="entry name" value="FHA_SNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000008">
        <property type="entry name" value="smad nuclear-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FHA" evidence="13">
        <location>
            <begin position="315"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="75"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="136"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="205"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002133877.2"/>
        </source>
    </evidence>
    <sequence length="433" mass="51028" checksum="784A01F711541790" modified="2020-08-12" version="1">MGKRRGTPGDQKENSGSSDEDQKMDEKNAPNLKKSKQRESRYSSSSSSSSRSSSNNDLSNSSAGKKQKRSKKKNRNTEGAEEIQEKRTRNLEKQSEETSQKVQEQRSKWDSPDRGNGGQRHNSHRNTKQRRHSRSYSRERERERETEKERHRDSDRDNPTRRDRSRREQRQRSPVQRQRARSNERKERPRRSPERRPERRSRSRSPRDRPNRDGRDFGRRKQQRRDNRSRGDDEHFEWGRKGDDKAPPDEEPVEKEKPNFGLSGALTEDTNKVNGVVVKYSEPVEARKPKRRWRLYPFKGETALPTLHIHRQSCFLVGRDRKVVDLAVDHPSCSKQHAALQYRLVPFEREDGSQGKRVRLYLIDLESANGTFLNNKKVDGRKYYELMEKDVIKFGFSSREYVLLHENSKEDQEDDDVHIKEERVDVPAEEVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZA8</accession>
    <name>A0A6I8UZA8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial cardiolipin hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6901270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133971.3">
        <property type="nucleotide sequence ID" value="XM_002133935.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:6901270"/>
    <dbReference type="InParanoid" id="A0A6I8UZA8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002133971.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="28372" checksum="90F3444FDE281954" modified="2020-08-12" version="1">MMPEIIISVCSLMGLCYSALVNLLSKLFGYFRIWTASSPRKSLRPYLYKIDLYVWQIIYGTPDTGHVVLFNGWHNGRTVVCDDCRLKRVGELIDQAKICLDVVLLSTANVQYLDRMLAAHNRGVNVRILASDKMLAANGPAMQLICALGVPVRGSPIGVKFGYNFAIIDSEKRALGLEATQDTVHTCLWRLRGNMKGCFRWILDVVKQKRDYRHDNYHYQIFFDEWDFLSPITPPYFAERHFVEVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZL5</accession>
    <name>A0A6I8UZL5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">L-seryl-tRNA(Sec) kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Pstk</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133663.2">
        <property type="nucleotide sequence ID" value="XM_002133627.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZL5"/>
    <dbReference type="FunCoup" id="A0A6I8UZL5">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902132"/>
    <dbReference type="InParanoid" id="A0A6I8UZL5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052648">
        <property type="entry name" value="Ser-tRNA(Sec)_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20873">
        <property type="entry name" value="L-SERYL-TRNA(SEC) KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20873:SF0">
        <property type="entry name" value="L-SERYL-TRNA(SEC) KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133663.2"/>
        </source>
    </evidence>
    <sequence length="280" mass="31957" checksum="1E0FAE9A7EF78CF1" modified="2020-08-12" version="1">MPPKICLLALIGLPGAGKSSLSNWLLSKQQDALHECQIVHLCYDDYINTELAYREQRIILIQILEKLIAYIQGAADWPPEVRRPTNNSRSCDYLILCDDNFYYRSMRYKLYQLCRTYGCIYSQIYIATPLASCLQANSTRGADCLPELVLRKMNDRLEPPGLEAWECSSLRLQNSDTESVSQLILTFILSLFHLPTALTPLVPSPTQTQMQSPAHCLDLALRASIKVQLQTLVDAKAKKLTSMKLNEKRKEILVQFRAKMSDKQTDQDSVDLNYYVNLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1H9</accession>
    <name>A0A6I8V1H9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Biogenesis of lysosome-related organelles complex 1 subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Blos3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134904.2">
        <property type="nucleotide sequence ID" value="XM_002134868.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1H9"/>
    <dbReference type="FunCoup" id="A0A6I8V1H9">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900516"/>
    <dbReference type="InParanoid" id="A0A6I8V1H9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V1H9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017245">
        <property type="entry name" value="BLOC-1_complex_su-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31974">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31974:SF2">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15753">
        <property type="entry name" value="BLOC1S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002134904.2"/>
        </source>
    </evidence>
    <sequence length="146" mass="16354" checksum="32FD048154F95104" modified="2020-08-12" version="1">MADLVRGEASESDEELQDQHKIFAAEVPGEATEEDDDDEDIYEQRRSTHVSSSTASIYRNNLLQRKLIENNIAIWRALTALTRNLVVNASKQLLTTDQMLIKSQLSMQSAHTSLQQAQRHVSELQSRTAAVMAGGSFLPHIHIRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2I3</accession>
    <name>A0A6I8V2I3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Venom acid phosphatase Acph-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6900262</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135591.3">
        <property type="nucleotide sequence ID" value="XM_002135555.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2I3"/>
    <dbReference type="FunCoup" id="A0A6I8V2I3">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900262"/>
    <dbReference type="InParanoid" id="A0A6I8V2I3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000038">
        <property type="entry name" value="venom acid phosphatase Acph-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF205">
        <property type="entry name" value="GH28721P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002135591.3"/>
        </source>
    </evidence>
    <sequence length="425" mass="49359" checksum="BC94098FFD28799E" modified="2020-08-12" version="1">MIFGRWTRKQILTMFGLGGAVVGAIIVIAVLNSGVSSKEMPMPWEDAATSQCHKTHPNTSHSTLELVHIVFRHGIRTPVDTYPNDPYLRDGFKPTGWGHVTNSGKRELYEIGRWLHRRYSDFMGPYYRPDRLHAQATASPRAMMSLQTTLASMFEPRGTAMEWNKKLNWQPIPIVSEPLDQDSLLLVRTPCPRYFEAWEEVFKRPEVIAETKPYEQMFRELTNLTGMAVKNAEDVNSLYITLLAEQEFGYELPAWTKDYFPDRMQFLAEQSYIYNAYTPEMQKIKGGPFLKRMYKEMSEKRDGSLKPKDRGMYIYAGHDWTVGNILSALGVWKRQMPRFAVMAIFETHRDKETGEYYMEIFLRNDEHGCLEQLKLRDCDRQCPLSRLIELSSEVLPNEPMEQRCRPHSPDFVEPPPRLIDQNAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V320</accession>
    <name>A0A6I8V320_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-uridine aminocarboxypropyltransferase 1</fullName>
            <ecNumber evidence="2">2.5.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DTW domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6896774</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs (acp3U(20)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62432"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:16092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82930"/>
            <dbReference type="EC" id="2.5.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TDD superfamily. DTWD1 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.25" evidence="2"/>
    <dbReference type="RefSeq" id="XP_002136980.2">
        <property type="nucleotide sequence ID" value="XM_002136944.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V320"/>
    <dbReference type="FunCoup" id="A0A6I8V320">
        <property type="interactions" value="1463"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896774"/>
    <dbReference type="InParanoid" id="A0A6I8V320"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016432">
        <property type="term" value="F:tRNA-uridine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005636">
        <property type="entry name" value="DTW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051521">
        <property type="entry name" value="tRNA_Mod/Golgi_Maint"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15627">
        <property type="entry name" value="NATURAL KILLER CELL-SPECIFIC ANTIGEN KLIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15627:SF8">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03942">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01144">
        <property type="entry name" value="DTW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DTW" evidence="12">
        <location>
            <begin position="43"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002136980.2"/>
        </source>
    </evidence>
    <sequence length="323" mass="37840" checksum="F8E7EF96B0E62B63" modified="2020-08-12" version="1">MSHPVEAQTRPRKYPFVHMRIADHSVLDSIEGRHVCRLCNRSRKFFCYSCHIPVEELEKLLPRLGLPLQIDIIKHKKEIDGKSTAVHAAVLAPEHVRIHTYPDIPDYRLDKDVVLIFPSAKSLTVPQLFERNVQLKIDDNYGLPKGHHMGTMLRRRVDEIEVLEDQPETTSKRSYTYGNLPVRRAVFIDSTWNQSRGIYADERVRGLRTVVLQNRLSQFWRHQRGSPRWYLATIEAIHQFVLEVHVNAWGLNSAYRGLDNLEITEGFHQLAAPLTREAPSEDVERESPYNGQYDNLLYFFANMYDLIHKYYDHNDLISYRRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3E1</accession>
    <name>A0A6I8V3E1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein argonaute-2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6897740</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137846.2">
        <property type="nucleotide sequence ID" value="XM_002137810.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3E1"/>
    <dbReference type="FunCoup" id="A0A6I8V3E1">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897740"/>
    <dbReference type="InParanoid" id="A0A6I8V3E1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="383"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="657"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002137846.2"/>
        </source>
    </evidence>
    <sequence length="985" mass="112900" checksum="D3F4D498DF3CB976" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3X1</accession>
    <name>A0A6I8V3X1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">4-coumarate--CoA ligase-like 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Acsx4</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138092.2">
        <property type="nucleotide sequence ID" value="XM_002138056.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3X1"/>
    <dbReference type="KEGG" id="dpo:6897995"/>
    <dbReference type="InParanoid" id="A0A6I8V3X1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000025">
        <property type="entry name" value="luciferin 4-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="39"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="440"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002138092.2"/>
        </source>
    </evidence>
    <sequence length="539" mass="59715" checksum="AEDEEDBD513BF528" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4B2</accession>
    <name>A0A6I8V4B2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138520.1">
        <property type="nucleotide sequence ID" value="XM_002138484.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4B2"/>
    <dbReference type="GeneID" id="6898478"/>
    <dbReference type="KEGG" id="dpo:6898478"/>
    <dbReference type="InParanoid" id="A0A6I8V4B2"/>
    <dbReference type="OMA" id="KHTVPSH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026126706" evidence="1">
        <location>
            <begin position="20"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138520.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="21724" checksum="15B753D2538A3FF2" modified="2020-08-12" version="1">MLIAGILWVLLLGSPQVESKFKSLHCTSLDKDYGEFLLCKIQAINRYRNSISISYLNKHTVPSHLTVRLEFFKRANGWRPFLYNITLNVCDFLDRNKTKPNNNNRIVFRLAAAYLIPYITRSYSCPFMENTVLKLVKLEFDVEKFRTRFPIETGEYALQFSLYHKKVLKYTVNGSMEYSNYREH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4D0</accession>
    <name>A0A6I8V4D0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DnaJ homolog l(2)tid, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Protein lethal(2)tumorous imaginal discs</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC6898514</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138555.2">
        <property type="nucleotide sequence ID" value="XM_002138519.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4D0"/>
    <dbReference type="FunCoup" id="A0A6I8V4D0">
        <property type="interactions" value="1960"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898514"/>
    <dbReference type="InParanoid" id="A0A6I8V4D0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V4D0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000003">
        <property type="entry name" value="dnaJ homolog subfamily A member 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000075">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="18">
        <location>
            <begin position="84"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="19">
        <location>
            <begin position="235"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="16">
        <location>
            <begin position="235"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="449"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="503"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_002138555.2"/>
        </source>
    </evidence>
    <sequence length="525" mass="57431" checksum="5F0A2C6C91E632A5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4I4</accession>
    <name>A0A6I8V4I4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898618</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138623.3">
        <property type="nucleotide sequence ID" value="XM_002138587.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4I4"/>
    <dbReference type="KEGG" id="dpo:6898618"/>
    <dbReference type="InParanoid" id="A0A6I8V4I4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026272714" evidence="2">
        <location>
            <begin position="23"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138623.3"/>
        </source>
    </evidence>
    <sequence length="292" mass="33067" checksum="96BE0D3251AF2CAA" modified="2020-08-12" version="1">MFKYINSLFLLALAFIAWTVCCYFHEIEEQLGTQGFDVELYSEHPALKYSQLPCKNIPKAVPQTFPTVELIFSSFIVLCLTYTWVKLSTFLVERTGQFWQAMKPEEEDRQVKEQLMERLNSLKAEFQMMSELLKQHQPSVDLELQTMSTLELQSHSGCSSLELQKCDSGCSEVHQLPSSSSCSNHCSNDVPINELCNQKNDVCSIRGTPRLPDVPTVDQDTESNYICTSQIPIGPNMFNTKPLNLELIRQSRLFARKPSVFLQVSDNFIAGPRDRPRIGGSSCTIALPPPNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4M0</accession>
    <name>A0A6I8V4M0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138508.1">
        <property type="nucleotide sequence ID" value="XM_002138472.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4M0"/>
    <dbReference type="GeneID" id="6898465"/>
    <dbReference type="KEGG" id="dpo:6898465"/>
    <dbReference type="InParanoid" id="A0A6I8V4M0"/>
    <dbReference type="OMA" id="ETWYIFV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246199">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138508.1"/>
        </source>
    </evidence>
    <sequence length="60" mass="6915" checksum="CCA4259DAFEDFD90" modified="2020-08-12" version="1">MVKKHKGTLAVIEQIYQDIPAFSDIFTEESFYTFAFCFVCATILVAFILSRFITIKPVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5T3</accession>
    <name>A0A6I8V5T3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Immune-induced peptide 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6898654</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bomanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138659.1">
        <property type="nucleotide sequence ID" value="XM_002138623.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5T3"/>
    <dbReference type="GeneID" id="6898654"/>
    <dbReference type="KEGG" id="dpo:6898654"/>
    <dbReference type="InParanoid" id="A0A6I8V5T3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246289">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013172">
        <property type="entry name" value="Bomanin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08194">
        <property type="entry name" value="DIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026221571" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002138659.1"/>
        </source>
    </evidence>
    <sequence length="40" mass="4191" checksum="21F45D93FB90F764" modified="2020-08-12" version="1">MKFLSLAFVLGLMAALANATPLNPGNVIINGDCRTCNVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6N9</accession>
    <name>A0A6I8V6N9_DROPS</name>
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        <submittedName>
            <fullName evidence="3">ATP-dependent RNA helicase DED1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC15382884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_004444279.1">
        <property type="nucleotide sequence ID" value="XM_004444222.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6N9"/>
    <dbReference type="GeneID" id="15382884"/>
    <dbReference type="KEGG" id="dpo:15382884"/>
    <dbReference type="InParanoid" id="A0A6I8V6N9"/>
    <dbReference type="OMA" id="ANSPINM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0265020">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026262565" evidence="1">
        <location>
            <begin position="21"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_004444279.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="11093" checksum="FFE0CCA50EF0FCE8" modified="2020-08-12" version="1">MNFLSLALIVVVCAVSSSWSLPVELDTGAISLLDLEAEQQPLVDETEAERVARGLGGFGGLGGKFGGLGGGLGGLKGGFGGGLGHGFGGGFGGHGFGGGFGSFKGLFDKKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7J8</accession>
    <name>A0A6I8V7J8_DROPS</name>
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        <submittedName>
            <fullName evidence="16">E3 SUMO-protein ligase PIAS2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Su(var)2-10</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_004444242.3">
        <property type="nucleotide sequence ID" value="XM_004444185.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V7J8"/>
    <dbReference type="FunCoup" id="A0A6I8V7J8">
        <property type="interactions" value="2553"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V7J8"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:SUMO ligase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097240">
        <property type="term" value="P:chromosome attachment to the nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16790">
        <property type="entry name" value="SP-RING_PIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.780:FF:000001">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000247">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000018">
        <property type="entry name" value="Uncharacterized protein, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF94">
        <property type="entry name" value="SUPPRESSOR OF VARIEGATION 2-10, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="12">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="PINIT" evidence="14">
        <location>
            <begin position="163"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="13">
        <location>
            <begin position="360"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="491"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="554"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_004444242.3"/>
        </source>
    </evidence>
    <sequence length="658" mass="72288" checksum="D2007C11EC85B467" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7S5</accession>
    <name>A0A6I8V7S5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Serine/threonine-protein phosphatase 1 regulatory subunit 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">PNUTS</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002132477.3">
        <property type="nucleotide sequence ID" value="XM_002132441.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035663.2">
        <property type="nucleotide sequence ID" value="XM_015180177.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V7S5"/>
    <dbReference type="KEGG" id="dpo:6903291"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072357">
        <property type="term" value="C:PTW/PP1 phosphatase complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Znf_CCCH"/>
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 10-RELATED"/>
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    <sequence length="1148" mass="122662" checksum="693AABF5D5FCCC14" modified="2020-08-12" version="1"> </sequence>
</entry>
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        <source>
            <dbReference type="RefSeq" id="XP_033236739.1"/>
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    <sequence length="212" mass="25768" checksum="38944EB25BC8AD67" modified="2020-08-12" version="1">MVFIKEWCRQIICLFRHNHDTLSKCTIMFVMLVGILDYSYVKSNCDLLWWKKQLTDLKVGPMFALLYIVTFKVSLILFMTFWSYTKKIDREQEKNDMPISEVKRRYRRFIMMRLLKAFNDLVEERHLLNLTHSGNVKKRFLKKHSKFIKEVELFRWNARKLYERTDDHLSLPLHQVLTVDDELEYQLLQSGYGDRLTMLRDVEIHQMVYGFQ</sequence>
</entry>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="9">
        <location>
            <begin position="86"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="8">
        <location>
            <begin position="186"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="298"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="571"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="829"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="761"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="840"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015039591.2"/>
        </source>
    </evidence>
    <sequence length="865" mass="95522" checksum="ECAAE32D89AB9421" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFH7</accession>
    <name>A0A6I8VFH7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Zinc transporter 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">ZnT41F</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <strain evidence="13">MV2-25</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <strain evidence="13">MV2-25</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015040233.2">
        <property type="nucleotide sequence ID" value="XM_015184747.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040234.2">
        <property type="nucleotide sequence ID" value="XM_015184748.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFH7"/>
    <dbReference type="KEGG" id="dpo:6898672"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VFH7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010043">
        <property type="term" value="P:response to zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF84">
        <property type="entry name" value="LD05335P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="436"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="11">
        <location>
            <begin position="164"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="12">
        <location>
            <begin position="469"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015040233.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015040234.2"/>
        </source>
    </evidence>
    <sequence length="543" mass="59057" checksum="43B900B8FDAF62A6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFX1</accession>
    <name>A0A6I8VFX1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26534449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040332.2">
        <property type="nucleotide sequence ID" value="XM_015184846.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFX1"/>
    <dbReference type="InParanoid" id="A0A6I8VFX1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VFX1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015040332.2"/>
        </source>
    </evidence>
    <sequence length="141" mass="16187" checksum="C604B111E28F1B84" modified="2020-08-12" version="1">MSWKCERQTIYIIAAVLGDILLIAFLRYVWHLSLFDVFVFLALILLNGVAIYVGYWISLEYPTLVLAGTLAIPLACIHLFVWRIDALTELWGRIKILTKIGHVLVLTFLIVYSALWMFLAFSYFNHVSNNTSLEILKEYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGV5</accession>
    <name>A0A6I8VGV5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040881.2">
        <property type="nucleotide sequence ID" value="XM_015185395.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGV5"/>
    <dbReference type="KEGG" id="dpo:26532486"/>
    <dbReference type="InParanoid" id="A0A6I8VGV5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VGV5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015040881.2"/>
        </source>
    </evidence>
    <sequence length="224" mass="25099" checksum="947DBF62E7201158" modified="2020-08-12" version="1">MAQVGKLNQWLRQSFSILKRVFQGDLFIKGRVHGPLPPTEIKNQTPSGKGKTSPEDKEPTVPIQGRSEADSVSVETAVEKAMPLPRMSRVIPPCRQDFEVLSTPKQDSIDKSSSTNAKDDTVTIAPVSSVDLLVDFFDTKYGKRAVQRDSMEITTTTALTNGPHPEMWDRRLRLKVIAQSNNICLSKELPKNDGLEQETLDENGKPSKARFCRTIYYKQRGPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJ85</accession>
    <name>A0A6I8VJ85_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26534456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042108.2">
        <property type="nucleotide sequence ID" value="XM_015186622.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VJ85"/>
    <dbReference type="KEGG" id="dpo:26534456"/>
    <dbReference type="InParanoid" id="A0A6I8VJ85"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015042108.2"/>
        </source>
    </evidence>
    <sequence length="127" mass="14257" checksum="0C012D9776DDFF11" modified="2020-08-12" version="1">MSSAFQKLSKVLAFCLANLLFGWYLWPMVLSDIPVWTNNEIYLNLLVYLHQLTVFWYGLLVLSECALEDLVLITLIILTDIVHISLSGAFLVHSAIGGGGDFRLSIVALLDMSLSFFCSIRWPFVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VK38</accession>
    <name>A0A6I8VK38_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sodium channel protein Nach</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ppk27</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043016.2">
        <property type="nucleotide sequence ID" value="XM_015187530.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VK38"/>
    <dbReference type="FunCoup" id="A0A6I8VK38">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532302"/>
    <dbReference type="InParanoid" id="A0A6I8VK38"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF237">
        <property type="entry name" value="PICKPOCKET 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015043016.2"/>
        </source>
    </evidence>
    <sequence length="430" mass="49285" checksum="3521455299B3F147" modified="2020-08-12" version="1">MSSRLVVALNRTVVEYFRKTSLNGFGLLYFIRRRRVQRIFWFSFICCGLIFAGYSVFAMILELLDNSTITDLSERDLGEKGALTLPSMAICSGYRFSKRKMQDYTQMLANSSGKPLGFWWQKIHLLSGYTDPMSVDPEEAKELQESLGFRDVRSQLMDLSTPCVSLILKCLQNRVTINCSEIFQLRATNHGHCCVLRDGNITGELSLSLDTSQEDEFPMEKSSFPGFRLHISSWLGRITIGGGEKSLVEVLGMELGANSELRDYPLDRRDCYFPHEGDGREKCLQDCRLRASLMNCHCVPYPFVRDAKLKQKSCTLEDIACLQMVAVNWSPNQCPQCLPLCNQMLYSLHKSLLGRMHPFRSTLLLRFGAPRWRIYSQDKHYHWYQILSNIGGVLGICIGCSLISGFELIYFMVFRFWSHFWHDPPNVSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMR7</accession>
    <name>A0A6I8VMR7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein unc-79 homolog isoform X6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">unc79</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232350.1">
        <property type="nucleotide sequence ID" value="XM_033376459.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VMR7">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VMR7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VMR7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024855">
        <property type="entry name" value="UNC79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21696">
        <property type="entry name" value="PROTEIN UNC-79 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21696:SF2">
        <property type="entry name" value="PROTEIN UNC-79 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14776">
        <property type="entry name" value="UNC-79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="695"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="845"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1000"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1384"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1554"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1844"/>
            <end position="1885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1918"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2061"/>
            <end position="2156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2213"/>
            <end position="2263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2276"/>
            <end position="2366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2811"/>
            <end position="2831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="846"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1018"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1390"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1435"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1461"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1583"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1875"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2070"/>
            <end position="2095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2121"/>
            <end position="2130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2213"/>
            <end position="2224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="2283"/>
            <end position="2293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2327"/>
            <end position="2336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2337"/>
            <end position="2347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2815"/>
            <end position="2824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232350.1"/>
        </source>
    </evidence>
    <sequence length="3050" mass="335243" checksum="9BC07CEEE89C7032" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNW5</accession>
    <name>A0A6I8VNW5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fatty acid-binding protein, muscle</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">M-FABP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fabp</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds fatty acids in a 1:1 molar ratio.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232722.1">
        <property type="nucleotide sequence ID" value="XM_033376831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNW5"/>
    <dbReference type="FunCoup" id="A0A6I8VNW5">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897819"/>
    <dbReference type="InParanoid" id="A0A6I8VNW5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247617">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="7">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00057009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00081149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033232722.1"/>
        </source>
    </evidence>
    <sequence length="131" mass="14550" checksum="C2D043AA8406E265" modified="2020-08-12" version="1">MASFVGKKYKLEKSENFDEYMKELGVGLVLRKMGNSISPTVEVTLEGDTYTMTTTSTFKTSAISFKLGEEFDEETLDGRQVKSIITLDGNKLTQEQKGDKPSTIVREFTDTDLTTTLTIGAVKSVRVYKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPK3</accession>
    <name>A0A6I8VPK3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7 8">Golgin subfamily A member 6-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8 9" type="primary">LOC6904114</name>
    </gene>
    <organism evidence="5 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7 8">MV-25-SWS-2005</strain>
            <strain evidence="5">MV2-25</strain>
            <tissue evidence="6 7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002136405.2">
        <property type="nucleotide sequence ID" value="XM_002136369.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232965.1">
        <property type="nucleotide sequence ID" value="XM_033377074.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232966.1">
        <property type="nucleotide sequence ID" value="XM_033377075.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232967.1">
        <property type="nucleotide sequence ID" value="XM_033377076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPK3"/>
    <dbReference type="KEGG" id="dpo:6904114"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043596">
        <property type="entry name" value="CFAP53/TCHP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183:SF2">
        <property type="entry name" value="TRICHOPLEIN KERATIN FILAMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183">
        <property type="entry name" value="TRICHOPLEIN KERATIN FILAMENT-BINDING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="359"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002136405.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033232965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033232966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033232967.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="58828" checksum="6E8B97C73427A756" modified="2020-08-12" version="1">MPGRLNQQHAAYAQRRELENNRVQATQAVNRYYDHWGKVTTRFENWTNKEYYENAEKKLQTRKSQKQKDIDLTDRKGRLRQLLDTENEAYDIEMGRKRRPRECSADTQMLGRVNQSLKEQEQLKRKLEMEAKLYGRWRHGVDDEKFLYQSKSDNEVLAKLNWLDKQIEQQKERDEQEALNAERKLQLQQEISRTELAQKERQLIQEQQIKEIRELQESHMVELRQHQSQAEKLKEEEQHFRLFIGELEKEKQLLEESTALVLQRPDTGQAFNLNKIKVFIRNRSEASRRQISLCINLLERMAKYVVRTEDLQSLLHKYKEQLESEQMAYTQIEAMYESEAKSSLQRCEENWREQHLQRYQELSKLIDTEKDCLDRLLQENVSQQQALLELRSTHLSGIEQATKKLEQITSGDQGSASSDPISVSSSTDLCAGALAEEDDASVNVPKVSNSFTNLNLDVWQDLPPVRGGINSPRLDKTRSLNVVTAQTALPPKFARKRVAWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VS78</accession>
    <name>A0A6I8VS78_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Zwei Ig domain protein zig-8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">dpr12</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233907.1">
        <property type="nucleotide sequence ID" value="XM_033378016.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233908.1">
        <property type="nucleotide sequence ID" value="XM_033378017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VS78"/>
    <dbReference type="KEGG" id="dpo:6898664"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001291">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001061">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF45">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 12, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634552" evidence="1">
        <location>
            <begin position="33"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="73"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="184"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033233907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033233908.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="36719" checksum="A9E4FA72E4E4304C" modified="2020-08-12" version="1">MLPPRLLLRLRRPMHLMELRILLLCLPTLLLATTLEPDQKSVLTDNDWKKLWMRGGISGDSKLDNSLDSSDSPMFEDSELMAHNTTVQLGGTAFLVCKVSGVDRVGVNWNQISWIRRRDWHILSSGAQLYTNDERFAILHTPGSNMWTLQIKFVQRRDHGMYECQVSTPTGIISHFVNLQVVVPEAFILGSGELHVDMGSTINLVCIIEKSPTPPQYVYWQKNDRLINYYDARRDISIETTPGPRTQSRLIIREPQVTDSGNYTCSASNTEPASIYVFVSKGDNMAAISRRKTSSADRLAHIFRSMLAPCLLLNTVVVRRIFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZ36</accession>
    <name>A0A6I8VZ36_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">NPC intracellular cholesterol transporter 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Npc1a</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>cholesterol(in) = cholesterol(out)</text>
            <dbReference type="Rhea" id="RHEA:39747"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="2A060601"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
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        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <end position="707"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="713"/>
            <end position="735"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="790"/>
            <end position="814"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="861"/>
            <end position="881"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1134"/>
            <end position="1152"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
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            <begin position="1159"/>
            <end position="1180"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1186"/>
            <end position="1208"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1229"/>
            <end position="1251"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1263"/>
            <end position="1288"/>
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        <location>
            <begin position="649"/>
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            <dbReference type="ARBA" id="ARBA00034049"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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            <dbReference type="RefSeq" id="XP_033236258.1"/>
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    <sequence length="1313" mass="147665" checksum="CE195858219A55E4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name evidence="5" type="primary">LOC4813160</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <property type="component" value="Chromosome X"/>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="29"/>
            <end position="615"/>
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        <location>
            <begin position="566"/>
            <end position="614"/>
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        <location>
            <begin position="355"/>
            <end position="383"/>
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        <location>
            <begin position="521"/>
            <end position="558"/>
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        <location>
            <begin position="371"/>
            <end position="383"/>
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    </feature>
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        <location>
            <begin position="535"/>
            <end position="548"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033238581.1"/>
        </source>
    </evidence>
    <sequence length="615" mass="69430" checksum="CA3ACBC652E75C0D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7P7</accession>
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        <submittedName>
            <fullName evidence="9">Myogenesis-regulating glycosidase isoform X1</fullName>
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        <name evidence="9" type="primary">LOC4814402</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="9">MV-25-SWS-2005</strain>
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        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
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    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
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    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
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    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
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    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF6">
        <property type="entry name" value="SITS-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="6">
        <location>
            <begin position="566"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="6">
        <location>
            <begin position="736"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="7">
        <location>
            <begin position="852"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="59"/>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="73"/>
            <end position="108"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="137"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
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        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033239307.1"/>
        </source>
    </evidence>
    <sequence length="941" mass="106631" checksum="7B4CC98C79E3A061" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7Q2</accession>
    <name>A0A6I8W7Q2_DROPS</name>
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        <recommendedName>
            <fullName evidence="1">Cyclin C-terminal domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="primary">LOC117184553</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238664.1">
        <property type="nucleotide sequence ID" value="XM_033382773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W7Q2"/>
    <dbReference type="KEGG" id="dpo:117184553"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="entry name" value="Cyclin-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin C-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238664.1"/>
        </source>
    </evidence>
    <sequence length="142" mass="16316" checksum="80452855B5F86827" modified="2020-08-12" version="1">MPGREHQFAKYPASTIAASSIAASMSGLKWHLRTGHNLPFLLGLLTDLTSIKQVQLQESMLHMETIFEEHSRNLQPFFVDIEPPNSAKMYCQTRYQTHPNQQYHQQRPQRMKKMPIPFLTTADANVDQNYFKANPTLSDANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8C9</accession>
    <name>A0A6I8W8C9_DROPS</name>
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        <submittedName>
            <fullName evidence="7">GA-binding protein subunit beta-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Atac3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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    </dbReference>
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        <property type="entry name" value="ADA2A-CONTAINING COMPLEX COMPONENT 3, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="89"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="122"/>
            <end position="154"/>
        </location>
    </feature>
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        <location>
            <begin position="155"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="256"/>
        </location>
    </feature>
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    </feature>
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        <location>
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        </location>
    </feature>
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            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033239537.1"/>
        </source>
    </evidence>
    <sequence length="593" mass="65315" checksum="E7AFF24362F09002" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9W2</accession>
    <name>A0A6I8W9W2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4780 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3 4" type="primary">LOC117184971</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
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    </dbReference>
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        <location>
            <begin position="9"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16012"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033240180.1"/>
        </source>
    </evidence>
    <sequence length="211" mass="23004" checksum="74E48FECFB16C951" modified="2020-08-12" version="1">MGWFQGNVKIIACEDERSVKLYKAAVAQVGEVYAGAKLVAVDWSLVPSRPRARIWVPATFKEPERILKMLQRCNPGLPTSDWKVAKVETTQGPTNQAVLVLNKESLAPIEAAKGELNFGFSSVTIKAYKSDAAAAALPVVNPDVQDVVAEIEAPDDELEPDQEGFSSETELMLDFESMCRERVSDDSDADITVVENLEDGPNDPSNKSPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCJ4</accession>
    <name>A0A6I8WCJ4_DROPS</name>
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        <submittedName>
            <fullName evidence="16 17">Potassium voltage-gated channel protein Shal isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">Shal</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17">MV-25-SWS-2005</strain>
            <tissue evidence="16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033240971.1">
        <property type="nucleotide sequence ID" value="XM_033385080.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240972.1">
        <property type="nucleotide sequence ID" value="XM_033385081.1"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:voltage-gated potassium channel complex"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005250">
        <property type="term" value="F:A-type (transient outward) potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18420">
        <property type="entry name" value="BTB_POZ_Shal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000016">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000004">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003975">
        <property type="entry name" value="K_chnl_volt-dep_Kv4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024587">
        <property type="entry name" value="K_chnl_volt-dep_Kv4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021645">
        <property type="entry name" value="Shal-type_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF105">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11879">
        <property type="entry name" value="DUF3399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11601">
        <property type="entry name" value="Shal-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01497">
        <property type="entry name" value="SHALCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="41"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033240971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033240972.1"/>
        </source>
    </evidence>
    <sequence length="571" mass="64518" checksum="F784112FF8EB7BFF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WE91</accession>
    <accession>B5DSA2</accession>
    <name>A0A6I8WE91_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Casein kinase II subunit alpha</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">CkIIalpha</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. May participate in Wnt signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033241751.1">
        <property type="nucleotide sequence ID" value="XM_033385860.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241752.1">
        <property type="nucleotide sequence ID" value="XM_033385861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WE91"/>
    <dbReference type="SMR" id="A0A6I8WE91"/>
    <dbReference type="KEGG" id="dpo:6899762"/>
    <dbReference type="OMA" id="ECHMIEW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243626">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="37"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033241751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033241752.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="39960" checksum="69D15F124427B21E" modified="2020-08-12" version="1">MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREIKILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNVMIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEELYAYLDKYNIDLDPRFHDILQRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHPYFLPIVNGQMNPNNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEI7</accession>
    <name>A0A6I8WEI7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117185141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240984.1">
        <property type="nucleotide sequence ID" value="XM_033385093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEI7"/>
    <dbReference type="KEGG" id="dpo:117185141"/>
    <dbReference type="InParanoid" id="A0A6I8WEI7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="11"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240984.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="36632" checksum="4AC72B1C05588C3D" modified="2020-08-12" version="1">MMGDLPTERSSFSRPFTHTGIDYAGPFEIRNYTGRACLITKGYVCVFVCFSTKAIHLEPTSDLTTEKFLAAFARFVARRGCPQRIHSDNGKTFVGAATLLSSDFLDAFKDSVTDAYSHQRVSWRFIPPGAPHMGGLWEAGVKSFKTLFYKATSTRRYTFEELSTLLAKIEACLNSRPLSPMSDDPTELLALTPGHFLIGGPLMSTAEPEIKGNLNSIINRWQHLKALNQQFCQRWKEEYLKELHKRTKWHTLTPNLQVGDMVVIKVDNLPSNEWRLGRITSVNPGADDRVRETVRELLRRELEGNCCRKKTPESSTSVIGRLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>B5DHZ9</accession>
    <accession>A0A6I8UXA8</accession>
    <name>B5DHZ9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Patsas</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_002132534.2">
        <property type="nucleotide sequence ID" value="XM_002132498.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DHZ9"/>
    <dbReference type="SMR" id="B5DHZ9"/>
    <dbReference type="FunCoup" id="B5DHZ9">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DHZ9"/>
    <dbReference type="GeneID" id="6902797"/>
    <dbReference type="KEGG" id="dpo:6902797"/>
    <dbReference type="eggNOG" id="KOG0509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031257_4_0_1"/>
    <dbReference type="InParanoid" id="B5DHZ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247228">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DHZ9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000335">
        <property type="entry name" value="Palmitoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000368">
        <property type="entry name" value="Palmitoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF17">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13606">
        <property type="entry name" value="Ank_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="441"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="538"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="493"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002132534.2"/>
        </source>
    </evidence>
    <sequence length="654" mass="73956" checksum="F4E2F553BCA8B408" modified="2016-01-20" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>B5DNY8</accession>
    <accession>A0A6I8VJ11</accession>
    <name>B5DNY8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation machinery-associated protein 7 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Coiled-coil domain-containing protein 72 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC6901137</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134613.1">
        <property type="nucleotide sequence ID" value="XM_002134577.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042365.1">
        <property type="nucleotide sequence ID" value="XM_015186879.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DNY8"/>
    <dbReference type="STRING" id="46245.B5DNY8"/>
    <dbReference type="GeneID" id="6901137"/>
    <dbReference type="KEGG" id="dpo:6901137"/>
    <dbReference type="eggNOG" id="KOG4766">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184661_2_0_1"/>
    <dbReference type="OMA" id="KKGPMNT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243671">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015157">
        <property type="entry name" value="TMA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28632">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09072">
        <property type="entry name" value="TMA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00031894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002134613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015042365.1"/>
        </source>
    </evidence>
    <sequence length="64" mass="6866" checksum="858C96AAAEDF01BE" modified="2008-10-14" version="1">MTGREGGKKKPLKAPKKDAKDMDDEEIAFKQKQKEQQKALDAAKANASKKGPLVGGGIKKSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>B5DQ34</accession>
    <accession>A0A6I8V1Q5</accession>
    <name>B5DQ34_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 216</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900687</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135051.2">
        <property type="nucleotide sequence ID" value="XM_002135015.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DQ34"/>
    <dbReference type="GeneID" id="6900687"/>
    <dbReference type="KEGG" id="dpo:6900687"/>
    <dbReference type="HOGENOM" id="CLU_2252803_0_0_1"/>
    <dbReference type="InParanoid" id="B5DQ34"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245231">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031720">
        <property type="entry name" value="DUF4728"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15860">
        <property type="entry name" value="DUF4728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002135051.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17029" checksum="0F2ADB988E730B63" modified="2016-01-20" version="2">MLRPENSLLIWAYIIAMFDLICASLFAGLCLDTIFEHLSWLTIFAVVFGIFWVSMIVVLLVGIYARNPRCLRAWILFSEGGILLEMCLLLYAFFSQSTFQAGLVRNGLFLMSGLIVELVFLYTIYQFYKALARCKRCKRPLESNSKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>B5DR25</accession>
    <accession>A0A6I8V197</accession>
    <name>B5DR25_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cuticle protein 10.6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135376.1">
        <property type="nucleotide sequence ID" value="XM_002135340.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DR25"/>
    <dbReference type="GeneID" id="6900011"/>
    <dbReference type="KEGG" id="dpo:6900011"/>
    <dbReference type="eggNOG" id="ENOG502T6Y2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119220_0_0_1"/>
    <dbReference type="InParanoid" id="B5DR25"/>
    <dbReference type="OMA" id="PAYSHGL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002135376.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="14915" checksum="5311535907F0643F" modified="2008-10-14" version="1">MFKYFVFAVFCLASAAAAPAPAPGYLGGLGAPSLPLAAAPAISYGHALAAPAYSHGLTAPSYAASYGLAPRISYAAPALAHAPLAAPAISAYGLGPRLSYASPALGHAPLGLGLGLAHAPLGLAHAPLGLAHAPLGLGYKSIAAPSYGLAHGW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-06-16" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q290S5</accession>
    <accession>Q5MBD9</accession>
    <name>TEF_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein teflon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tef</name>
        <name type="ORF">GA21437</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2006" name="Genetics" volume="174" first="125" last="134">
            <title>Molecular characterization of teflon, a gene required for meiotic autosome segregation in male Drosophila melanogaster.</title>
            <authorList>
                <person name="Arya G.H."/>
                <person name="Lodico M.J.P."/>
                <person name="Ahmad O.I."/>
                <person name="Amin R."/>
                <person name="Tomkiel J.E."/>
            </authorList>
            <dbReference type="PubMed" id="16816414"/>
            <dbReference type="DOI" id="10.1534/genetics.106.061556"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically required in males for proper segregation of autosomal bivalents at meiosis I. Expression is required in the male germ line prior to spermatocyte stage S4. May have a role as a bridging molecule maintaining adhesion to hold autosome bivalents together via heterochromatic connections.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <text evidence="2">Male meiotic chromosomes.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Drosophilid specific gene duplication generates rgr and tef. Teflon has a function unique to Drosophilids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Teflon family.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL25287" version="2"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AY840221">
        <property type="protein sequence ID" value="AAW24439.2"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL25287.2"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q290S5"/>
    <dbReference type="FunCoup" id="Q290S5">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q290S5"/>
    <dbReference type="eggNOG" id="ENOG502T9CV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="Q290S5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030849">
        <property type="term" value="C:autosome"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051308">
        <property type="term" value="P:male meiosis chromosome separation"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0159">Chromosome partition</keyword>
    <keyword id="KW-0469">Meiosis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000377410" description="Protein teflon">
        <location>
            <begin position="1"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C2H2-type 1" evidence="3">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C2H2-type 2" evidence="3">
        <location>
            <begin position="649"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C2H2-type 3" evidence="3">
        <location>
            <begin position="677"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="339"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="339"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q7K4M4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EAL25287.2"/>
        </source>
    </evidence>
    <sequence length="703" mass="80060" checksum="4978F0DA7ACFF480" modified="2009-06-16" version="3"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-03-02" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q296V2</accession>
    <name>UFL1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>E3 UFM1-protein ligase 1 homolog</fullName>
            <ecNumber>2.3.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">E3 UFM1-protein transferase 1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GA10722</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">E3 UFM1-protein ligase that mediates ufmylation of target proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UFL1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.-"/>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL28355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q296V2"/>
    <dbReference type="SMR" id="Q296V2"/>
    <dbReference type="FunCoup" id="Q296V2">
        <property type="interactions" value="2593"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q296V2"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0285850">
        <property type="protein sequence ID" value="FBpp0284288"/>
        <property type="gene ID" value="FBgn0070778"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802251"/>
    <dbReference type="eggNOG" id="KOG2235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012417_1_1_1"/>
    <dbReference type="InParanoid" id="Q296V2"/>
    <dbReference type="OMA" id="CILHASG"/>
    <dbReference type="PhylomeDB" id="Q296V2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070778">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061666">
        <property type="term" value="F:UFM1 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032434">
        <property type="term" value="P:regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018611">
        <property type="entry name" value="Ufl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056761">
        <property type="entry name" value="Ufl1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056580">
        <property type="entry name" value="Ufl1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056579">
        <property type="entry name" value="Ufl1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057:SF0">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09743">
        <property type="entry name" value="E3_UFM1_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23659">
        <property type="entry name" value="UFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25041">
        <property type="entry name" value="UFL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25870">
        <property type="entry name" value="WHD_UFL1_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="chain" id="PRO_0000391885" description="E3 UFM1-protein ligase 1 homolog">
        <location>
            <begin position="1"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O94874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="785" mass="87553" checksum="796AD4AF3621A7AD" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CA0</accession>
    <name>CP2B_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Cardio acceleratory peptide 2b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Capability protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Myotropin-CAP2b-like protein</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>CAP-1</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>CAP-2</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>CAP-3</fullName>
            </recommendedName>
            <alternativeName>
                <fullName>Pyrokinin-1</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">capa</name>
        <name type="ORF">GA13779</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CAP-1 and CAP-2, but not CAP-3 are ligands for the Capa receptor. CAP-1 and CAP-2 are probably components of the signal transduction pathway that leads to Malpighian tubule fluid secretion via the second messenger nitric oxide.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pyrokinin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL26746.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001357612.2">
        <property type="nucleotide sequence ID" value="XM_001357575.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29CA0"/>
    <dbReference type="FunCoup" id="Q29CA0">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29CA0"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0284510">
        <property type="protein sequence ID" value="FBpp0282948"/>
        <property type="gene ID" value="FBgn0073815"/>
    </dbReference>
    <dbReference type="GeneID" id="4800261"/>
    <dbReference type="KEGG" id="dpo:4800261"/>
    <dbReference type="CTD" id="43541"/>
    <dbReference type="eggNOG" id="ENOG502TAG0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1697372_0_0_1"/>
    <dbReference type="InParanoid" id="Q29CA0"/>
    <dbReference type="OMA" id="SMLVHIV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073815">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016084">
        <property type="term" value="F:myostimulatory hormone activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013231">
        <property type="entry name" value="Periviscerokinin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08259">
        <property type="entry name" value="Periviscerokin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00539">
        <property type="entry name" value="PYROKININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0027">Amidation</keyword>
    <keyword id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword id="KW-0527">Neuropeptide</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000339244" evidence="1">
        <location>
            <begin position="27"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_0000339245" description="CAP-1" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000339246" evidence="1">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_0000339247" description="CAP-2" evidence="1">
        <location>
            <begin position="88"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000339248" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_0000339249" description="CAP-3" evidence="1">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000339250" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="modified residue" description="Valine amide" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="modified residue" description="Valine amide" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="modified residue" description="Leucine amide" evidence="1">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9NIP6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <sequence length="155" mass="16958" checksum="4572C392A8F91481" modified="2008-06-10" version="2" precursor="true">MKAIFSLYNIVSAILLLVLLAEFSTAELNHDKNRRGANMGLYAFPRVGRSDPSLANSLRDASDAAVFDGLYGDASQEDYNEADYQKRAGLVAFPRVGRSDAELRKFAHLLALQQVLDKRTGPSASSGLWFGPRLGKRSVDAKAFSDASKGQQEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CT8</accession>
    <accession>A0A6I8UAF4</accession>
    <name>Q29CT8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytochrome c oxidase subunit NDUFA4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ND-MLRQ</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352358.1">
        <property type="nucleotide sequence ID" value="XM_001352322.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29CT8"/>
    <dbReference type="FunCoup" id="Q29CT8">
        <property type="interactions" value="244"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29CT8"/>
    <dbReference type="GeneID" id="4811790"/>
    <dbReference type="KEGG" id="dpo:4811790"/>
    <dbReference type="eggNOG" id="ENOG502S8MJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181002_0_0_1"/>
    <dbReference type="InParanoid" id="Q29CT8"/>
    <dbReference type="OMA" id="PWQEYAD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076785">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29CT8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256:SF1">
        <property type="entry name" value="GEO09626P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001352358.1"/>
        </source>
    </evidence>
    <sequence length="83" mass="9450" checksum="DABB39EA42CBB79A" modified="2006-04-04" version="1">MQGLGLQSLKKNPALIPLYVCVAAGAIGAVYYMGRLATRNPDVTWNRSTNPEPWQEYKDKQYKFYSPVRDYSKTKSAAPNYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DX2</accession>
    <accession>A0A6I8UBC4</accession>
    <name>Q29DX2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Single domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4812122</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352791.2">
        <property type="nucleotide sequence ID" value="XM_001352755.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DX2"/>
    <dbReference type="FunCoup" id="Q29DX2">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="GeneID" id="4812122"/>
    <dbReference type="KEGG" id="dpo:4812122"/>
    <dbReference type="eggNOG" id="ENOG502T8XQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158197_0_0_1"/>
    <dbReference type="InParanoid" id="Q29DX2"/>
    <dbReference type="OMA" id="MPGCEEL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072828">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DX2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029277">
        <property type="entry name" value="SVWC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053308">
        <property type="entry name" value="Vago-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957">
        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39957:SF1">
        <property type="entry name" value="AT09846P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15430">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01318">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Single" evidence="3">
        <location>
            <begin position="38"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001352791.2"/>
        </source>
    </evidence>
    <sequence length="117" mass="12939" checksum="9E9397A0CBF19FB5" modified="2008-10-14" version="2">MHSEKVCAAFALLACLALVEAAVYSQPAIFHPAHPGKCFDKFTRRAMLPDKEYKPKGICASMTCSLEAREISIETCPYVEAPGCEELPADVNWSFPKCCPQFKCVDFKTGKEFVVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FT5</accession>
    <accession>A0A6I8W5D0</accession>
    <name>Q29FT5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Flad1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033238543.1">
        <property type="nucleotide sequence ID" value="XM_033382652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FT5"/>
    <dbReference type="SMR" id="Q29FT5"/>
    <dbReference type="FunCoup" id="Q29FT5">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FT5"/>
    <dbReference type="KEGG" id="dpo:4814266"/>
    <dbReference type="eggNOG" id="KOG2644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056971_2_0_1"/>
    <dbReference type="InParanoid" id="Q29FT5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078171">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FT5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="14">
        <location>
            <begin position="110"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="14">
        <location>
            <begin position="190"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033238543.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="34770" checksum="33FA50FD0930D4F4" modified="2016-01-20" version="3">MLRRTACLRHLNRRTAVYGSRTVQSSFVMSKCCCTATTTITTPVLNSSPPRHNSVLTLNGGKSMATGRTPAAPSPHIAVTPETRARIEQRQQTAFDFFDETLQLYGVEELIFCFNGGKDCTVLLDLLMRYCRQQGISSREIPMLYIKSDDAFAEIDEFVARCVEHYNVQLIEYEESLKEALTHMSADMPRIKAVFVGSRNTDPYCEHLSPMQPTDNGWPPMMRLNPLLEWSYHDVWHYIHMNCVAYCPLYNKGYTSIGYRSNTVPNPHLKRLDLTSPCSGAEGDYRPAWELADPTQERDGRLQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q29I72</accession>
    <accession>A0A6I8UHR8</accession>
    <name>Q29I72_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hsc70-interacting protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4815909</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer. Interacts with Hsc70 as well as DNAJ homologs and Hsp90.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355722.1">
        <property type="nucleotide sequence ID" value="XM_001355686.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29I72"/>
    <dbReference type="SMR" id="Q29I72"/>
    <dbReference type="FunCoup" id="Q29I72">
        <property type="interactions" value="1823"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29I72"/>
    <dbReference type="GeneID" id="4815909"/>
    <dbReference type="KEGG" id="dpo:4815909"/>
    <dbReference type="eggNOG" id="KOG1308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026202_0_1_1"/>
    <dbReference type="InParanoid" id="Q29I72"/>
    <dbReference type="OMA" id="YEKRRYK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075555">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14438">
        <property type="entry name" value="Hip_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000112">
        <property type="entry name" value="FAM10 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3420:FF:000001">
        <property type="entry name" value="Hsc70-interacting protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034649">
        <property type="entry name" value="Hip_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45883">
        <property type="entry name" value="HSC70-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45883:SF2">
        <property type="entry name" value="HSC70-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18253">
        <property type="entry name" value="HipN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="257"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="382"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="257"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355722.1"/>
        </source>
    </evidence>
    <sequence length="427" mass="45484" checksum="0F70AC61F8481D33" modified="2006-04-04" version="1">MAAPMEKDDLVKLKSFIEFVDSNPTVLNVPQLKFVKDFIEKFGGQVPAGDFKMPDAAAGGKCPFGGDAGPKSKSAGAGVSTPDSEGVSIDSDASEDDDSLSESESDVELDMEGVIEPDLDPAQPMGDSSKEPTEEEMDQAGDVRGEAAKAYGEQKFEEAVGLYTKAIELNPGNALYYAKRGQAFLKMKKPNACIRDCDKALELNCDSAAGYKFRGRAHRLLGQFEEAAKDLRQACKLDFDEETDEWLREVTPNAKKIEQHRVKQERKQAERKVKDRLRAQNKARKEQEKQKASASSTGGGFPGGAGGGFPGGFPGGFPDMGGAGGIPNGASIAEMLGLMKDPEMAAAIRDILANPANISKYASNPKIFNLMKQFFPSGDPTGAGFGGAGAGAGLFGQENDEKEKASDNSEPKAPKKESADFVDDGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IR7</accession>
    <accession>A0A6I8UHF4</accession>
    <name>Q29IR7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mpv17-like protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4815690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355527.1">
        <property type="nucleotide sequence ID" value="XM_001355491.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IR7"/>
    <dbReference type="FunCoup" id="Q29IR7">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IR7"/>
    <dbReference type="GeneID" id="4815690"/>
    <dbReference type="KEGG" id="dpo:4815690"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049109_4_1_1"/>
    <dbReference type="InParanoid" id="Q29IR7"/>
    <dbReference type="OMA" id="IICHYWY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074113">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IR7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061668">
        <property type="term" value="P:mitochondrial ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF8">
        <property type="entry name" value="MPV17-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001355527.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="27273" checksum="B7F4D11FAADFC652" modified="2006-04-04" version="1">MQSLRCQARILFSTAASSGRLIRLNLLRNVATGSGTGTGSGTGSGSGFRSRAKLMQKLREWHARAFSNRFLLFTNVGISLTLSCLGDVMEQHLEIYSGEIERFDSLRTSHMATSGVTVGIICHFWYKMLDKRMPGRSMRVVAKKIVLDQLICSPVYISVFFVTLGLLEQKDKHEVWDEIKDKAWKLYAAEWTVWPAAQFINFYWIPTHYRIFYDNIISLGYDVLTSKVKHTKSHIKKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LL9</accession>
    <accession>A0A6I8UKL2</accession>
    <name>Q29LL9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polycomb protein esc</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein extra sex combs</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">esc</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat ESC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356960.1">
        <property type="nucleotide sequence ID" value="XM_001356924.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LL9"/>
    <dbReference type="SMR" id="Q29LL9"/>
    <dbReference type="FunCoup" id="Q29LL9">
        <property type="interactions" value="1260"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LL9"/>
    <dbReference type="GeneID" id="4817553"/>
    <dbReference type="KEGG" id="dpo:4817553"/>
    <dbReference type="eggNOG" id="KOG1034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032683_1_0_1"/>
    <dbReference type="InParanoid" id="Q29LL9"/>
    <dbReference type="OMA" id="VWEMDPS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073406">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LL9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000056">
        <property type="entry name" value="Polycomb protein eed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051243">
        <property type="entry name" value="PcG_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10253">
        <property type="entry name" value="POLYCOMB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001356960.1"/>
        </source>
    </evidence>
    <sequence length="424" mass="47643" checksum="93BDCB82864A5AAB" modified="2006-04-04" version="1">MSSDKVKNGNEHDSDDSCGDESASFTTNSTTSQSKSPTSSTRSKRRGRRSTKSKPKTVAAYKYDTHAKENHGASIFGVAFNTLLGKDEPQMFATAGSNRCTVYECPRKGGLQLVHCYADPDPDEVFYTCAWSYDLKSSAPLLAAAGYRGVVRVIDIEQNEAVGNYVGHGQAINELKFHPHKLQLLLSGSKDHAIRLWNIQTHVCIAIFGGVEAHRDEVLSIDFNMKGDRIVSSGMDHSLKLWCLDSPDFQHKVELSNTFSQEKSTMPFPTVTRHFPDFSTRDIHRNYVDCVQWFGNFVLSKSCENAIICWKPGQLHQSFEQVKPNDSSCTLIATFSYDECEIWFVRFGFNPWHKVIALGNQHGKVYVWELDPSDPEGAHMTTLHNQRSVSTVRQIAFSRDASILVYVCDDATVWRWNRPQAAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PC7</accession>
    <accession>A0A6I8UL80</accession>
    <name>Q29PC7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Equilibrative nucleoside transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Ent2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357297.1">
        <property type="nucleotide sequence ID" value="XM_001357261.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PC7"/>
    <dbReference type="FunCoup" id="Q29PC7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="GeneID" id="4817861"/>
    <dbReference type="KEGG" id="dpo:4817861"/>
    <dbReference type="eggNOG" id="KOG1479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021611_0_1_1"/>
    <dbReference type="InParanoid" id="Q29PC7"/>
    <dbReference type="OMA" id="WVYWGIA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076573">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF80">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001357297.1"/>
        </source>
    </evidence>
    <sequence length="455" mass="51393" checksum="A5894545D44859F7" modified="2006-04-04" version="1">MAEGKSEKLPFIGKQAPKPVTLNPSWESELPPADSNGKGSVLAKLGLPPKDRYKIVFFIFMLHGLGTLMPWNMFITAKSYFEDFKLGANNTVPTEVNYRSTFMQNMGFASQIPNLLFNWLNIFVNFGGDLTSRIVYSIIFEVVILLVTIVLAMMDSSQWPGIFFWATMVSIVLLNICNGIYQNTIYGIVASLPIKYTGAVVLGSNISGVFTTVMSIICTAFFDSKRTSAIYYFVTAIVILLLCFDTYFALPVNKFFRHYDQICQSNEKKSDSRAELNVPYWQIFKKASPQLFNIFLTFFVTLSVFPAIHANIKASDEDFFISKDYFTLFTCFLTFNVFAMLGSLTTSWVQWPGPKFLVVPVALRLVFIPLFIMCNYIPPDMTRTLEVYFPNEWVYWGIAVIMSYSSGYLSSLGMMYAPQTVHAKYQTTAGMYAAAMLITGIFSGVMFSYLGPLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PN7</accession>
    <accession>A0A6I8ULA1</accession>
    <name>Q29PN7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase PPP1R11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein phosphatase 1 regulatory subunit 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4817883</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357187.2">
        <property type="nucleotide sequence ID" value="XM_001357151.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PN7"/>
    <dbReference type="FunCoup" id="Q29PN7">
        <property type="interactions" value="326"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29PN7"/>
    <dbReference type="GeneID" id="4817883"/>
    <dbReference type="KEGG" id="dpo:4817883"/>
    <dbReference type="eggNOG" id="KOG4102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098333_1_0_1"/>
    <dbReference type="InParanoid" id="Q29PN7"/>
    <dbReference type="OMA" id="MKSKCCC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072729">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29PN7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00031039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357187.2"/>
        </source>
    </evidence>
    <sequence length="156" mass="17280" checksum="233E04B7FF8B4BEB" modified="2008-10-14" version="2">MAQRTNNKPSNGTATEETDGSLPETTPTLHLRLEQPRDERRVIFHEGVIDNEHLNRKKSKCCCIYKKPLAFGESSSEDDEECENCFGHPEKRKRNARHNHNHDNDNEPSTSAQASAQPGPSVGDPISPPAEPVVSPVKPKSDPTTPIDQFEQTGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZZ1</accession>
    <accession>A0A6I8UD41</accession>
    <name>Q2LZZ1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Biogenesis of lysosome-related organelles complex 1 subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Blos2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353632.2">
        <property type="nucleotide sequence ID" value="XM_001353596.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZZ1"/>
    <dbReference type="FunCoup" id="Q2LZZ1">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZZ1"/>
    <dbReference type="GeneID" id="4813816"/>
    <dbReference type="KEGG" id="dpo:4813816"/>
    <dbReference type="eggNOG" id="KOG4559">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110820_1_0_1"/>
    <dbReference type="InParanoid" id="Q2LZZ1"/>
    <dbReference type="OMA" id="PMMQQID"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072833">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZZ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353632.2"/>
        </source>
    </evidence>
    <sequence length="164" mass="18228" checksum="A0DAC21D4FFCDAFF" modified="2008-10-14" version="2">MDTEKPSTSAAAAASAQVMQDQNPLLDSPLHGPTLSSSSSFEALARHDPNLSRLATQMFQKTEEYITHELNAPLEDYRLLEEMNKATIAKYKDMRQIAENLNASTSDLSLKFQQLAPLMQQIDEISDTVDKLEAAAYKLDAYSIALENRVKCVLQRKAGTHVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M133</accession>
    <accession>A0A6I8UCD2</accession>
    <name>Q2M133_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Pickpocket protein 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ppk26</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353240.2">
        <property type="nucleotide sequence ID" value="XM_001353204.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M133"/>
    <dbReference type="FunCoup" id="Q2M133">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="GeneID" id="4813908"/>
    <dbReference type="KEGG" id="dpo:4813908"/>
    <dbReference type="eggNOG" id="KOG4294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024950_1_1_1"/>
    <dbReference type="InParanoid" id="Q2M133"/>
    <dbReference type="OMA" id="IIHEYCD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000008">
        <property type="entry name" value="pickpocket protein 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001353240.2"/>
        </source>
    </evidence>
    <sequence length="596" mass="67928" checksum="3D5E9A6666BADC17" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NSW6</accession>
    <accession>A0A6I8V8Q9</accession>
    <name>A0A0R3NSW6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26532779</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036152.1">
        <property type="nucleotide sequence ID" value="XM_015180666.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NSW6"/>
    <dbReference type="GeneID" id="26532779"/>
    <dbReference type="KEGG" id="dpo:26532779"/>
    <dbReference type="InParanoid" id="A0A0R3NSW6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271766">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015036152.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="14109" checksum="8A6F24D5E06D4FBD" modified="2016-01-20" version="1">MCLSSEYDKICTWCFAVWNIILGLTWSILGYLAVIGHEHGLHSRYYDIVVCLYTVTVCICAPLHLLSGILIIVGDWKDSQQTFKVGKNLSNLFPFFLLGTIIFPVIHFIGLGRVCSYYEKRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NTE7</accession>
    <accession>A0A6I8V8X7</accession>
    <name>A0A0R3NTE7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein CG42266 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036289.1">
        <property type="nucleotide sequence ID" value="XM_015180803.2"/>
    </dbReference>
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        <property type="entry name" value="Formin_Homology_Diaphanous_sf"/>
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        <property type="match status" value="1"/>
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            <begin position="34"/>
            <end position="357"/>
        </location>
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        <location>
            <begin position="392"/>
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            <begin position="38"/>
            <end position="57"/>
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            <begin position="65"/>
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            <begin position="115"/>
            <end position="130"/>
        </location>
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        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
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        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
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            <begin position="259"/>
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        </location>
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            <begin position="316"/>
            <end position="334"/>
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            <begin position="396"/>
            <end position="422"/>
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            <begin position="423"/>
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            <begin position="436"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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            <dbReference type="RefSeq" id="XP_015036289.1"/>
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    <sequence length="629" mass="70454" checksum="20C48FBA369D923C" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
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    <evidence type="ECO:0000313" key="1">
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            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015036804.1"/>
        </source>
    </evidence>
    <sequence length="408" mass="44349" checksum="FB15BFD9B39E756F" modified="2016-01-20" version="1">MSVSVVVGASACSFPSHPHTTKAHQYSGAACQAGPLRVASFRESDSMRWLRKAFGNRSTAGTWLRFQFRFDTKPLWGYKSAPLTTDSIQSEVWNRLGFYNPYTVYFDSISALLPWQVPTLSTMKVFICLAALLVASACASKTEGEKVPLEKKLDKRGLLDLGYGYGHAGLDVGYLGHGSVAGHGYGHGYGLTGHSAPAAIAVGHSGPAYSIGHSGPAVAVHHAPAPYVISKQADVHKTITITKGIPVPVHVDRPYPVVHEKRVPVEVKVPVPQPYEVIRKVPVTVKEYVKVPVPVPQPYEVIRHEKYPVHVPVDRPVPVEVPRPYPVPVAKPYPVYVEKAVKVAVPVQVDRPYPVYVKVPVVSHSVVKHAPTVAVSSYPVHGYPVSSYPASSYGHDASVYADHSGYHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NX57</accession>
    <accession>A0A6I8VY91</accession>
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        <submittedName>
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        </submittedName>
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        <name evidence="4" type="primary">LOC4816910</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <property type="entry name" value="TSEC-2-RELATED"/>
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            <end position="812"/>
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        <location>
            <begin position="121"/>
            <end position="194"/>
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            <end position="329"/>
        </location>
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            <begin position="572"/>
            <end position="599"/>
        </location>
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        <location>
            <begin position="635"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="765"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="789"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033236066.1"/>
        </source>
    </evidence>
    <sequence length="853" mass="96573" checksum="C0ED42B20EF173BA" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAK1</accession>
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        <submittedName>
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        <name evidence="9" type="primary">LOC4812003</name>
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            <tissue evidence="9">Whole body</tissue>
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        <text evidence="2">Monomer.</text>
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        <subcellularLocation>
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        </subcellularLocation>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DUF1907"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015021">
        <property type="entry name" value="C11orf54_DUF1907"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13204:SF1">
        <property type="entry name" value="ESTER HYDROLASE C11ORF54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13204">
        <property type="entry name" value="PTD012 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08925">
        <property type="entry name" value="DUF1907"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117856">
        <property type="entry name" value="AF0104/ALDC/Ptd012-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="33"/>
            <end position="314"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001352468.3"/>
        </source>
    </evidence>
    <sequence length="326" mass="36128" checksum="22CAFA66B2270051" modified="2020-08-12" version="1">MSQKQTQNQLETEQLLFEEKPLHVPPLPELQNVIQGALRGNFAQVEVSVGACPDLKAPRFGLVESGLGGRPTLLEAGGPPFLLPNVHREKLYNIKEITRKIQGPGKVFAVGAGAGPWPIRSSNCEGIFNLSVSEGDELTNGSYTATVRGEQEVCVLEKIPHTEPRCALLLNLFLSQGAPGQVLKISAKQRTGEHNFIECIRKGLEDHYGDRVVGLGGIFLIKKGAVHQHVMPDFSSTPIHTDEELNEWLKFYDMPAQLNAVGTLVTKEHGLDLRLQHFHSFSFSNWGGHYHYDTTPDIVEYEAYLNVAERVVRVDKPLSTHKVGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAN3</accession>
    <name>A0A6I8UAN3_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">Staphylococcal nuclease domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Tudor-SN</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352493.2">
        <property type="nucleotide sequence ID" value="XM_001352457.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAN3"/>
    <dbReference type="SMR" id="A0A6I8UAN3"/>
    <dbReference type="FunCoup" id="A0A6I8UAN3">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
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        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
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        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
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    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SNase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017179">
        <property type="entry name" value="RISC-Tudor-SN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TNase-like" evidence="8">
        <location>
            <begin position="24"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="8">
        <location>
            <begin position="196"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="8">
        <location>
            <begin position="347"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="8">
        <location>
            <begin position="536"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="7">
        <location>
            <begin position="751"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001352493.2"/>
        </source>
    </evidence>
    <sequence length="928" mass="103412" checksum="1EC38A3192473CAE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBQ0</accession>
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        <submittedName>
            <fullName evidence="18">Mucolipin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Trpml</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
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        <reaction evidence="12">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352972.2">
        <property type="nucleotide sequence ID" value="XM_001352936.4"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:endosome membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:lysosomal membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0072345">
        <property type="term" value="F:NAADP-sensitive calcium-release channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mucolipin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCLN_ECD"/>
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    <dbReference type="InterPro" id="IPR039031">
        <property type="entry name" value="Mucolipin"/>
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        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12127">
        <property type="entry name" value="MUCOLIPIN"/>
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        <property type="entry name" value="SD02261P"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PKD_channel"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
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    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="450"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="555"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Mucolipin extracytosolic" evidence="16">
        <location>
            <begin position="152"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="15">
        <location>
            <begin position="453"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001352972.2"/>
        </source>
    </evidence>
    <sequence length="659" mass="74796" checksum="FE5A0CF7CD07E668" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UC55</accession>
    <name>A0A6I8UC55_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">General transcription factor IIH subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">mrn</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Part of TBP-based Pol II pre-initiation complex (PIC), in which Pol II core assembles with general transcription factors and other specific initiation factors including GTF2E1, GTF2E2, GTF2F1, GTF2F2, TCEA1, ERCC2, ERCC3, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2A1, GTF2A2, GTF2B and TBP; this large multi-subunit PIC complex mediates DNA unwinding and targets Pol II core to the transcription start site where the first phosphodiester bond forms.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TFB2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353141.4">
        <property type="nucleotide sequence ID" value="XM_001353105.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UC55"/>
    <dbReference type="FunCoup" id="A0A6I8UC55">
        <property type="interactions" value="1085"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812973"/>
    <dbReference type="InParanoid" id="A0A6I8UC55"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080565">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UC55">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2610:FF:000001">
        <property type="entry name" value="General transcription factor IIH subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040662">
        <property type="entry name" value="Tfb2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004598">
        <property type="entry name" value="TFIIH_p52/Tfb2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00625">
        <property type="entry name" value="tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152">
        <property type="entry name" value="TFIIH, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03849">
        <property type="entry name" value="Tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18307">
        <property type="entry name" value="Tfb2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Tfb2 C-terminal" evidence="11">
        <location>
            <begin position="422"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001353141.4"/>
        </source>
    </evidence>
    <sequence length="496" mass="56604" checksum="EBB402D86F63A08F" modified="2020-08-12" version="1">MAMADTKSGRYASAGSSNSVVPLVQGPANLECKDFQEYLRTRQTAETLEKLYNYPPICLAVFRELPELARQFIIRILFVDQPVPQAVVTSWGAQRFAKEQTEATSCLSDLNVWRVTAIPGGLSAWELSPTFKKSVRQVLLGGGKPWPMTNTLEKDSKPRDIAFLDSYAMSRWRCVLHYMVGTGNRNGTDAEAISPDAVRILLHANLMKRDERDGITITRQGFQFLLLDTRAQVWHFMLQYLDTCEERGVSLPECLSMLFQLSFSTLGRDYSSEGMNSQMLSFLQHLREFGLVFQRKRKEGRFYPTRLALNVTSKEAATTMTVSDEEAMQDSGYIVVETNYRVYAYTDSPLQVAVLGLFTELLYRFPNLVVGVLTRDSVRQALRGGITAEQIVSYLQQYAHPNMKLVESAIQSKSCLPPTVVDQIKLWEMERNRFTYTEGVVYNQFLSQNDFVTLRDYAQSIHVLVWQNERTRTMVVQKHGHDDVKRYWKKYSKGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UC87</accession>
    <name>A0A6I8UC87_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4813700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353182.2">
        <property type="nucleotide sequence ID" value="XM_001353146.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UC87"/>
    <dbReference type="FunCoup" id="A0A6I8UC87">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813700"/>
    <dbReference type="InParanoid" id="A0A6I8UC87"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026353616" evidence="2">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353182.2"/>
        </source>
    </evidence>
    <sequence length="132" mass="14822" checksum="A6AB288DD7B0E0F9" modified="2020-08-12" version="1">MRAWCVLSLIGCLGIALISSKPLEEAGDIAQEDSEKAYEHTYLTIDPTAEDARDEEEELGEATGDDDKAGGEGKAYYIEVEDDENDDSTVDKPIEEEPINFLKYPEHETDHKPYPRFAILKNGFVNHVNLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCX8</accession>
    <name>A0A6I8UCX8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="16">1.3.8.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">2-methyl branched chain acyl-CoA dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">2-methylbutyryl-coenzyme A dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">LOC4812765</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="32">MV-25-SWS-2005</strain>
            <tissue evidence="32">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(2R)-2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = ethylacryloyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:65296"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:156439"/>
            <dbReference type="ChEBI" id="CHEBI:156440"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:65297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(2S)-2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48256"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57337"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:88166"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57336"/>
            <dbReference type="ChEBI" id="CHEBI:57337"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:43781"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>2-methylpropanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 2-methylpropenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:44180"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57338"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62500"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:44181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:24005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>valproyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-propylpent-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:65344"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:156457"/>
            <dbReference type="ChEBI" id="CHEBI:156458"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:65345"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 27">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="15">Amino-acid degradation; L-isoleucine degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.5" evidence="16"/>
    <dbReference type="RefSeq" id="XP_001353478.3">
        <property type="nucleotide sequence ID" value="XM_001353442.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCX8"/>
    <dbReference type="FunCoup" id="A0A6I8UCX8">
        <property type="interactions" value="1363"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812765"/>
    <dbReference type="InParanoid" id="A0A6I8UCX8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCX8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003853">
        <property type="term" value="F:short-chain 2-methyl fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046395">
        <property type="term" value="P:carboxylic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01158">
        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000012">
        <property type="entry name" value="Acyl-CoA dehydrogenase short/branched chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase short/branched chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
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        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF1">
        <property type="entry name" value="SHORT_BRANCHED CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 27" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7 27" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 27" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="30">
        <location>
            <begin position="39"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="29">
        <location>
            <begin position="154"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="28">
        <location>
            <begin position="261"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_001353478.3"/>
        </source>
    </evidence>
    <sequence length="415" mass="45469" checksum="3CC3AC1AD03EBA7B" modified="2020-08-12" version="1">MMNSLKKLPIRMLVNAAARQQNAAMSSATGLPPPLTFLTEDEKMMKETVAKLAQDQILPLVKKMDHEHKFDPSVVKAVFDNGLMGIEIDTELGGSGCNFMTNIIVVEELSKIDPAVAAFVDIHNTLVNSLMIKFGNAEQKAKYLPKLAQEYAGSFALTEPGAGSDAFSLKTVAKKDGSHYVINGTKMWISNSDVAGVFLVFANAKPEDGYRGITTFIVDRETPGLIVNKPEDKLGIRASGTCMLTFDNVRVPEENILGTFGHGYKYAAGFLNEGRIGIGAQMVGLAQGTFDATIPYLLERKQFGESIYNFQSMQHQIATIATEIEAARLMTYNAARLQEQGVPFLKEAAMAKFYASEVAQRAAIKCIDWMGGVGFTRDFPQEKFYRDVKIGAIYEGTSNMQLSTIAKCVKKEYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDZ4</accession>
    <name>A0A6I8UDZ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Larval serum protein 1 gamma chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4812945</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353976.4">
        <property type="nucleotide sequence ID" value="XM_001353940.4"/>
    </dbReference>
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        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812945"/>
    <dbReference type="InParanoid" id="A0A6I8UDZ4"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1520">
        <property type="entry name" value="Hemocyanin, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.10">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013788">
        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000896">
        <property type="entry name" value="Hemocyanin/hexamerin_mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005203">
        <property type="entry name" value="Hemocyanin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037020">
        <property type="entry name" value="Hemocyanin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005204">
        <property type="entry name" value="Hemocyanin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036697">
        <property type="entry name" value="Hemocyanin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511:SF5">
        <property type="entry name" value="FAT-BODY PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511">
        <property type="entry name" value="LARVAL STORAGE PROTEIN/PHENOLOXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03723">
        <property type="entry name" value="Hemocyanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00372">
        <property type="entry name" value="Hemocyanin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03722">
        <property type="entry name" value="Hemocyanin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48050">
        <property type="entry name" value="Hemocyanin, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0758">Storage protein</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026320865" evidence="2">
        <location>
            <begin position="23"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="Hemocyanin N-terminal" evidence="4">
        <location>
            <begin position="33"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Hemocyanin middle" evidence="3">
        <location>
            <begin position="161"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Hemocyanin C-terminal" evidence="5">
        <location>
            <begin position="440"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353976.4"/>
        </source>
    </evidence>
    <sequence length="682" mass="80328" checksum="6B36EA215C53B9D5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG83</accession>
    <name>A0A6I8UG83_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NADH-cytochrome b5 reductase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4814540</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355001.4">
        <property type="nucleotide sequence ID" value="XM_001354965.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UG83"/>
    <dbReference type="FunCoup" id="A0A6I8UG83">
        <property type="interactions" value="421"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814540"/>
    <dbReference type="InParanoid" id="A0A6I8UG83"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UG83">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF184">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="7">
        <location>
            <begin position="68"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355001.4"/>
        </source>
    </evidence>
    <sequence length="328" mass="36963" checksum="162CD2F1D396D449" modified="2020-08-12" version="1">MLALFPPHFDPEWTNTVSMTADGEQKVEDAFTIQESDCCGNGCTNCILDNKPKPSQRALLAGKRNIILKYTRFRLLQKDSHKGDDKVLFLHLAADSDEKDDCVLDIPPGHHVMLRIGSLLRPYSPYWTDFASKEFKILVKLQPDGPMSKNLAAMQLNDRLEFRGPIGNYRHDPAVTKCIFILAQGVAIAPTLPLVGQVLENEDDMSRIWHLVCSPDLQHVYFRDQLLEFSKYWNYRSCLYLPHQQCGSAECLGAGHCIQECVELRRSLRYKERVCLHRLDASELESHANPAIPGLRVLIIAGDSGFQRAMGEAAARALAVAQENVYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGW4</accession>
    <name>A0A6I8UGW4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-acyltransferase like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4815556</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355304.3">
        <property type="nucleotide sequence ID" value="XM_001355268.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGW4"/>
    <dbReference type="KEGG" id="dpo:4815556"/>
    <dbReference type="InParanoid" id="A0A6I8UGW4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF15">
        <property type="entry name" value="GH19286P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026095756" evidence="2">
        <location>
            <begin position="18"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="508"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="531"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="587"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="616"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="Nose resistant-to-fluoxetine protein N-terminal" evidence="3">
        <location>
            <begin position="41"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001355304.3"/>
        </source>
    </evidence>
    <sequence length="661" mass="75133" checksum="BEA6239B66B0D4D3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHF3</accession>
    <name>A0A6I8UHF3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL14m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L14, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mRpL14</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355539.2">
        <property type="nucleotide sequence ID" value="XM_001355503.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHF3"/>
    <dbReference type="FunCoup" id="A0A6I8UHF3">
        <property type="interactions" value="389"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815436"/>
    <dbReference type="InParanoid" id="A0A6I8UHF3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHF3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000004">
        <property type="entry name" value="39S ribosomal protein L14, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L14, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355539.2"/>
        </source>
    </evidence>
    <sequence length="165" mass="18190" checksum="EFDE6C4FE04B2089" modified="2020-08-12" version="1">MALQILRRLTTTLPVATITASARAVTCRQQQLLHTTPACCEIRKLARLRVVDNSDLGKRAMAEGRPPRCIHVYNKRGVGYIGDKVLVAIKGQMKKGILVGLKQNQKPKQPKFDSNNLVLIDDNGSPLGTRIHVPIPTVLRTILKEKTLAKGADYTKVLAIASRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHJ5</accession>
    <name>A0A6I8UHJ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pyridoxal phosphate phosphatase PHOSPHO2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4815527</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355497.2">
        <property type="nucleotide sequence ID" value="XM_001355461.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHJ5"/>
    <dbReference type="FunCoup" id="A0A6I8UHJ5">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815527"/>
    <dbReference type="InParanoid" id="A0A6I8UHJ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHJ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16418">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006384">
        <property type="entry name" value="HAD_hydro_PyrdxlP_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016965">
        <property type="entry name" value="Pase_PHOSPHO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01489">
        <property type="entry name" value="DKMTPPase-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20889:SF12">
        <property type="entry name" value="LP01149P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20889">
        <property type="entry name" value="PHOSPHATASE, ORPHAN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06888">
        <property type="entry name" value="Put_Phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031051">
        <property type="entry name" value="PyrdxlP_Pase_PHOSPHO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355497.2"/>
        </source>
    </evidence>
    <sequence length="294" mass="33572" checksum="87A9CC794EBC14ED" modified="2020-08-12" version="1">MSSSSAAVAEAVACCRLEKQQRRRLAAFDFDHTIVAQNTDTVVRDLLPTEVVSSRALNELVENDCWTEYMSEVFRLLHEQQVTEARIRDTIRVIPEVPGFVRLIKHLHKKLNFDMIIISDSNSVFIDEWLKAHNLADCFVAIFTNPAEFDSNGQLQVRPHHQQSECKLSASNLCKGRVLEHFVIEQDLRRSIRYDHVFYVGDGNNDICPVLRQRACDFACARQGFAMEKHLIRNRNKLKLRAHLLVWKSGFDLMEQMLALPQMQVSQVDLETPEGQAEMARRASAVAGPTKPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHQ4</accession>
    <name>A0A6I8UHQ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18">Zinc finger and BTB domain-containing protein 14-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">LOC4815791</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17 18">MV-25-SWS-2005</strain>
            <tissue evidence="17 18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355704.4">
        <property type="nucleotide sequence ID" value="XM_001355668.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240718.1">
        <property type="nucleotide sequence ID" value="XM_033384827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHQ4"/>
    <dbReference type="KEGG" id="dpo:4815791"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001963">
        <property type="entry name" value="Replication initiator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000325">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000931">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="15">
        <location>
            <begin position="84"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="501"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="529"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="299"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="620"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="620"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001355704.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033240718.1"/>
        </source>
    </evidence>
    <sequence length="675" mass="76132" checksum="B48BB8D2ADD41DD8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHR7</accession>
    <name>A0A6I8UHR7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNL-type zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4815802</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355684.3">
        <property type="nucleotide sequence ID" value="XM_001355648.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHR7"/>
    <dbReference type="FunCoup" id="A0A6I8UHR7">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815802"/>
    <dbReference type="InParanoid" id="A0A6I8UHR7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHR7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF13">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="5">
        <location>
            <begin position="85"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001355684.3"/>
        </source>
    </evidence>
    <sequence length="178" mass="19900" checksum="E400C893C76CCB39" modified="2020-08-12" version="1">MSTFRQLYNLSAGMGRRALQVATRIAQNEARTHSHFQFQVQFPTRLASSTTAVPVWTGTNTCTCRGKPVKDAGENKATNSIPLGELQTKMQLIYTCKICQTRNMKTISKVAYQRGVVIVTCEGCANHHLIADNLNWFTDLNGKRNIEEILAEKGEKVIKMVDGNCEFLPEDNKPKPNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIM1</accession>
    <name>A0A6I8UIM1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13 14">ATP-binding cassette sub-family G member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13 14" type="primary">LOC4816302</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13 14">MV-25-SWS-2005</strain>
            <tissue evidence="12 13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355992.3">
        <property type="nucleotide sequence ID" value="XM_001355956.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036625.2">
        <property type="nucleotide sequence ID" value="XM_015181139.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036626.2">
        <property type="nucleotide sequence ID" value="XM_015181140.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIM1"/>
    <dbReference type="SMR" id="A0A6I8UIM1"/>
    <dbReference type="KEGG" id="dpo:4816302"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001077">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF32">
        <property type="entry name" value="PROTEIN WHITE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="582"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="15"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355992.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015036625.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015036626.2"/>
        </source>
    </evidence>
    <sequence length="611" mass="68890" checksum="00E65DD03405953C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK88</accession>
    <name>A0A6I8UK88_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356847.4">
        <property type="nucleotide sequence ID" value="XM_001356811.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK88"/>
    <dbReference type="KEGG" id="dpo:4817153"/>
    <dbReference type="InParanoid" id="A0A6I8UK88"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026259127" evidence="1">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356847.4"/>
        </source>
    </evidence>
    <sequence length="248" mass="28506" checksum="3445294C19FDDADC" modified="2020-08-12" version="1">MIGKSLPLFLLLLGPSIIEALGTQVVCYDNKRMLSLIEGLNGTVDEQLSLAAFTWGKLRADYPRDVAKIEDLDETMQHVMCSKDKNITLSQHLQGHLLREHIREHLETLNSTALFKQALESPEHELGKWQLAIGRHSRRFHCLFKPAQLQKIIGSVLKRLYRLEKASAMAAFLYQLYMVGNKDSFQLMVYSELLLYERYKSGGQQRPQFGSYMAKMWQSLQMEEPYATLDQAVKQRLLDAIIDLLGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM48</accession>
    <name>A0A6I8UM48_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">wts</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001357595.4">
        <property type="nucleotide sequence ID" value="XM_001357558.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM48"/>
    <dbReference type="FunCoup" id="A0A6I8UM48">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800311"/>
    <dbReference type="InParanoid" id="A0A6I8UM48"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007444">
        <property type="term" value="P:imaginal disc development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051093">
        <property type="term" value="P:negative regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042308">
        <property type="term" value="P:negative regulation of protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022604">
        <property type="term" value="P:regulation of cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046620">
        <property type="term" value="P:regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21778">
        <property type="entry name" value="MobB_LATS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05598">
        <property type="entry name" value="STKc_LATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000391">
        <property type="entry name" value="Large tumor suppressor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000086">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000199">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049761">
        <property type="entry name" value="LATS1-like_MobB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF418">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE WARTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="756"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="20">
        <location>
            <begin position="1058"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="132"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="554"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="658"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="918"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1073"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="304"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="582"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="626"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="665"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="786"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001357595.4"/>
        </source>
    </evidence>
    <sequence length="1142" mass="124380" checksum="6BA3C859865A8112" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM77</accession>
    <name>A0A6I8UM77_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein Rpp14b</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">uncharacterized protein LOC4800291</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4800291</name>
        <name evidence="4 5" type="synonym">Rpp14b</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <strain evidence="3 4">MV2-25</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357644.2">
        <property type="nucleotide sequence ID" value="XM_001357607.4"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033379237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM77"/>
    <dbReference type="GeneID" id="4800291"/>
    <dbReference type="KEGG" id="dpo:4800291"/>
    <dbReference type="OMA" id="PCHFQVI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073820">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357644.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235128.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="12989" checksum="7BCA974BC7B1D375" modified="2020-08-12" version="1">MSDYYYFDVKLKLRNPEAIIFTPIFFRSCVLNALESFFGDLGGKTNMDIVKFCDKQQRIVFRVPEEFFERTRTALSLIGHYQEVPCHFQVIGTSKTALDFDKDISATAARRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM99</accession>
    <name>A0A6I8UM99_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TsetseEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4800486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357753.2">
        <property type="nucleotide sequence ID" value="XM_001357716.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM99"/>
    <dbReference type="FunCoup" id="A0A6I8UM99">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="GeneID" id="4800486"/>
    <dbReference type="KEGG" id="dpo:4800486"/>
    <dbReference type="InParanoid" id="A0A6I8UM99"/>
    <dbReference type="OMA" id="AEFCVNY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075977">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007931">
        <property type="entry name" value="TsetseEP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05267">
        <property type="entry name" value="DUF725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026207210" description="Protein TsetseEP domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Protein TsetseEP" evidence="2">
        <location>
            <begin position="48"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357753.2"/>
        </source>
    </evidence>
    <sequence length="197" mass="21566" checksum="6C9D4DDE0E7B9824" modified="2020-08-12" version="1">MFSRASTLLLVFVALAAAAPSSDIASSTSLIDFMLQSQSLRTATTLNLDCFDWYLPILKGHSDQYEADYKVCADRFNASKWLIDTSYSIARNGISSKAKETCAALERCDYEVENSKAFECFAATAPEQSAILTIIGQNATDFNKQLLAELALVDSDLDTCQTKAERAYKESSGATFDELSACLEDEDWAQPTTTVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN48</accession>
    <name>A0A6I8UN48_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Trissin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Trissin</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358131.2">
        <property type="nucleotide sequence ID" value="XM_001358094.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN48"/>
    <dbReference type="FunCoup" id="A0A6I8UN48">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="GeneID" id="4800947"/>
    <dbReference type="KEGG" id="dpo:4800947"/>
    <dbReference type="InParanoid" id="A0A6I8UN48"/>
    <dbReference type="OMA" id="LRERRHN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073348">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026019710" evidence="1">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358131.2"/>
        </source>
    </evidence>
    <sequence length="104" mass="12187" checksum="B74CE774B24A2C4C" modified="2020-08-12" version="1">MHWLAHFQIILLCIWLMCCSSNAMPCDSCGKECANACGTKHFRTCCFNYLRKRNDPDELRRSSDRRLIDFILLQGRALYTQELRERHHNGTLIDGTLGLQTYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNC8</accession>
    <name>A0A6I8UNC8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair protein RAD51 homolog 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">spn-D</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358289.3">
        <property type="nucleotide sequence ID" value="XM_001358252.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNC8"/>
    <dbReference type="FunCoup" id="A0A6I8UNC8">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801152"/>
    <dbReference type="InParanoid" id="A0A6I8UNC8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033065">
        <property type="term" value="C:Rad51C-XRCC3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000707">
        <property type="term" value="P:meiotic DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19492">
        <property type="entry name" value="Rad51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052093">
        <property type="entry name" value="HR_Repair_Mediator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3 RAD51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="8">
        <location>
            <begin position="27"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001358289.3"/>
        </source>
    </evidence>
    <sequence length="255" mass="28921" checksum="E6D4AE983361D61E" modified="2020-08-12" version="1">METTFLSSNNINLFNKSCLDILRGEIQSKKIATGHKSLDRHLGGGISLRQITELVGNSGTGKTKMCLQLCLNVQIPKSAGGLEGAALFIDTRQDFHPDRLQELARDLEKEYKQRAPDFQAAKMLQNVHYVSCPNAVQLMATVLSSYRHLIAHPNIKVIVIDSLAFSLRMLDDGAQRFELLLELVESMRRLLREHEVAWVVTNVLTHRCIGDQFHVVPALGEMYSHLINERIWFSRNGHCFLGKSWKTSRLIKDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNH9</accession>
    <name>A0A6I8UNH9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein uL11m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">39S ribosomal protein L11, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mRpL11</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358236.1">
        <property type="nucleotide sequence ID" value="XM_001358199.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNH9"/>
    <dbReference type="FunCoup" id="A0A6I8UNH9">
        <property type="interactions" value="892"/>
    </dbReference>
    <dbReference type="GeneID" id="4801077"/>
    <dbReference type="KEGG" id="dpo:4801077"/>
    <dbReference type="InParanoid" id="A0A6I8UNH9"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077411">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000008">
        <property type="entry name" value="39S ribosomal protein L11, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000003">
        <property type="entry name" value="39S ribosomal protein L11, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 10 14" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="12">
        <location>
            <begin position="25"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="11">
        <location>
            <begin position="88"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001358236.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="21558" checksum="59762EF001B92719" modified="2020-08-12" version="1">MSKAAGKLKSLKKTVERVTHTSKLKTNIPAGMAAAGPPLGPMLGQRAINIAAFCKDFNTKTAEMIEGVPLPCRISVNSDRSYDLAIHHPPATFFLKQAAGIQRGTMTPGKEVAGMITLKHLYEIAAIKIQDPPNALLSMQQMCEMLISIARTCGIKVVRDLDPAAYGEFLEERKTIVEQQRRELQEKREAKMLRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNP5</accession>
    <name>A0A6I8UNP5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Mitogen-activated protein kinase kinase kinase 4</fullName>
            <ecNumber evidence="4">2.7.11.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">MAPK/ERK kinase kinase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">Mekk1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">MV-25-SWS-2005</strain>
            <tissue evidence="23">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001358448.3">
        <property type="nucleotide sequence ID" value="XM_001358411.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UNP5">
        <property type="interactions" value="2236"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801337"/>
    <dbReference type="InParanoid" id="A0A6I8UNP5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06626">
        <property type="entry name" value="STKc_MEKK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000122">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045801">
        <property type="entry name" value="MEKK4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19431">
        <property type="entry name" value="MEKK4_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="1285"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="24"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="515"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="573"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1124"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="515"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="576"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1128"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1139"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="1314"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_001358448.3"/>
        </source>
    </evidence>
    <sequence length="1572" mass="176758" checksum="D9EAF8A030ED7107" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNP8</accession>
    <name>A0A6I8UNP8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="11">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PGS1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001358447.2">
        <property type="nucleotide sequence ID" value="XM_001358410.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNP8"/>
    <dbReference type="FunCoup" id="A0A6I8UNP8">
        <property type="interactions" value="1687"/>
    </dbReference>
    <dbReference type="GeneID" id="4801336"/>
    <dbReference type="KEGG" id="dpo:4801336"/>
    <dbReference type="InParanoid" id="A0A6I8UNP8"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080535">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="216"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001358447.2"/>
        </source>
    </evidence>
    <sequence length="536" mass="61350" checksum="CF1339CFEDBA93B8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPE1</accession>
    <name>A0A6I8UPE1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Neprilysin-11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Nepl19</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358743.4">
        <property type="nucleotide sequence ID" value="XM_001358706.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPE1"/>
    <dbReference type="FunCoup" id="A0A6I8UPE1">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801696"/>
    <dbReference type="InParanoid" id="A0A6I8UPE1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF238">
        <property type="entry name" value="FI07649P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026030014" evidence="9">
        <location>
            <begin position="30"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="11">
        <location>
            <begin position="81"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="10">
        <location>
            <begin position="480"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001358743.4"/>
        </source>
    </evidence>
    <sequence length="682" mass="77711" checksum="1998A3719084528B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ61</accession>
    <name>A0A6I8UQ61_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Natterin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4802188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359156.1">
        <property type="nucleotide sequence ID" value="XM_001359119.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ61"/>
    <dbReference type="GeneID" id="4802188"/>
    <dbReference type="KEGG" id="dpo:4802188"/>
    <dbReference type="InParanoid" id="A0A6I8UQ61"/>
    <dbReference type="OMA" id="HFSHGAI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076013">
        <property type="expression patterns" value="Expressed in adult organism and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006616">
        <property type="entry name" value="DM9_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31649">
        <property type="entry name" value="AGAP009604-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31649:SF10">
        <property type="entry name" value="IP19903P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11901">
        <property type="entry name" value="DM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00696">
        <property type="entry name" value="DM9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001359156.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="16102" checksum="F07C30E5C9D44E48" modified="2020-08-12" version="1">MDGHSWLHYSHGTVPQNAVVAGHDSDGDTIYVGRAFYCNDLLPAKVIPNKGKAYVAYANQEVELESYEALSGNNYEWLSAENGEVPPGAVSVGHNVDGEALYAGRGYHAGALTVGKVHPSHGCLYIPYDGEEVKIFAYEVLSRRMMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQT6</accession>
    <name>A0A6I8UQT6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">NADH-quinone oxidoreductase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ND-23</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359257.1">
        <property type="nucleotide sequence ID" value="XM_001359220.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQT6"/>
    <dbReference type="SMR" id="A0A6I8UQT6"/>
    <dbReference type="FunCoup" id="A0A6I8UQT6">
        <property type="interactions" value="1509"/>
    </dbReference>
    <dbReference type="GeneID" id="4802317"/>
    <dbReference type="KEGG" id="dpo:4802317"/>
    <dbReference type="InParanoid" id="A0A6I8UQT6"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077803">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
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        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004538">
        <property type="entry name" value="PRK05888.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004539">
        <property type="entry name" value="PRK05888.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF20">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="109"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="148"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359257.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="24725" checksum="6DBD5DF5FC118FCE" modified="2020-08-12" version="1">MSLTMRIFTASRNGQRMFRTNGACLLAAKRTEPKDIVEVPKGYVYVNNKELSMEMEDITDRAATSMFFGELFRGFGVTLAHIFKEPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEERADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLCNGDKWESEIASNLQADHLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URN9</accession>
    <name>A0A6I8URN9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 33B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Vps33B</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359847.4">
        <property type="nucleotide sequence ID" value="XM_001359810.4"/>
    </dbReference>
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        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803046"/>
    <dbReference type="InParanoid" id="A0A6I8URN9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="562"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359847.4"/>
        </source>
    </evidence>
    <sequence length="646" mass="71784" checksum="1BC6EDC85A99567B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URS0</accession>
    <name>A0A6I8URS0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4802938</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001359753.1">
        <property type="nucleotide sequence ID" value="XM_001359716.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URS0"/>
    <dbReference type="SMR" id="A0A6I8URS0"/>
    <dbReference type="FunCoup" id="A0A6I8URS0">
        <property type="interactions" value="1365"/>
    </dbReference>
    <dbReference type="GeneID" id="4802938"/>
    <dbReference type="KEGG" id="dpo:4802938"/>
    <dbReference type="InParanoid" id="A0A6I8URS0"/>
    <dbReference type="OMA" id="AMHQYKV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071574">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13986">
        <property type="entry name" value="STKc_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000890">
        <property type="entry name" value="Blast:Serine/threonine-protein kinase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052239">
        <property type="entry name" value="Ser/Thr-specific_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="28"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359753.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="36355" checksum="3A65450FB321889A" modified="2020-08-12" version="1">MQSIGWTLIMKRGCFCRKETLNINGSRYTIRERLAQGGFSLIDLGENPATRRSYAIKRITCHSIDDQNIALREIENCRKIDSENVIRVVDYELKGQADIVINTTSTLYIVLPYYKHGALLDHLQLRARKQDHMPEAQIVQIFLGVCEGVKAIHEAKPVPLAHRDLKTANICLSDSFEPIIVDLGSMTEARLQIVSQSDAQRLQDEAEERSSIVYRAPELFSVKSYCTIDERTDIWSLGCVLYAMCYFNSPFDPIYERGDSVALAVLSGNINIPEDSIYTEDMHDLINYMLRTDPMERPFIYSVIERTHDLIQKLEGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US85</accession>
    <name>A0A6I8US85_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Xanthine dehydrogenase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">AOX2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
        <text evidence="18">Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="18">Binds 2 [2Fe-2S] clusters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the xanthine dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360074.4">
        <property type="nucleotide sequence ID" value="XM_001360037.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8US85">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803310"/>
    <dbReference type="InParanoid" id="A0A6I8US85"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000009">
        <property type="entry name" value="Aldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000013">
        <property type="entry name" value="Aldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.50:FF:000003">
        <property type="entry name" value="Aldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.50:FF:000003">
        <property type="entry name" value="Aldehyde oxidase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.120:FF:000007">
        <property type="entry name" value="indole-3-acetaldehyde oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000012">
        <property type="entry name" value="Xanthine dehydrogenase/oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000127">
        <property type="entry name" value="Xanthine_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="CO dehydrogenase flavoprotein C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 18" id="KW-0408">Iron</keyword>
    <keyword evidence="12 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 18" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="19">
        <location>
            <begin position="15"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="20">
        <location>
            <begin position="218"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="1226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="753"/>
        </location>
        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="899"/>
        </location>
        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="985"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000127-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000127-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000127-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001360074.4"/>
        </source>
    </evidence>
    <sequence length="1282" mass="141438" checksum="47C00C5CCFB734A7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USJ7</accession>
    <name>A0A6I8USJ7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L40, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">mRpL40</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL40 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359984.3">
        <property type="nucleotide sequence ID" value="XM_001359947.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239747.1">
        <property type="nucleotide sequence ID" value="XM_033383856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USJ7"/>
    <dbReference type="KEGG" id="dpo:4803202"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3440:FF:000001">
        <property type="entry name" value="Mitochondrial ribosomal protein L40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019192">
        <property type="entry name" value="Ribosomal_mL40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039145">
        <property type="entry name" value="Ribosomal_mL40_metazoa/plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13359">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L40, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13359:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09812">
        <property type="entry name" value="MRP-L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359984.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033239747.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="22279" checksum="9DA445C499B34693" modified="2020-08-12" version="1">MSLISAFARLGLQRGGVAGPAVARCLHTTPVLCAEPLKKKKKLDPQVVKQREDRRKKKIEKQIRRLEKNARQLKPVEELEVPLELIDEKDKRQRKLTPLGSAELEERALLKKQWARYKHDERVSDFQIIDRLVMSQNKALEELRRESEELYQAAIEVDLQLLPVSLKGPVASPPIKNYASPDGDYIHQSMKWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USR9</accession>
    <name>A0A6I8USR9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein late bloomer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Tsp42Eo</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360185.1">
        <property type="nucleotide sequence ID" value="XM_001360148.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USR9"/>
    <dbReference type="SMR" id="A0A6I8USR9"/>
    <dbReference type="FunCoup" id="A0A6I8USR9">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="GeneID" id="4803467"/>
    <dbReference type="KEGG" id="dpo:4803467"/>
    <dbReference type="InParanoid" id="A0A6I8USR9"/>
    <dbReference type="OMA" id="RYEKWVH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071893">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000048">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360185.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="24515" checksum="5B1347C13F0CB4E4" modified="2020-08-12" version="1">MPTIRVCLQWTAVVFNTITLIIGILAALAGVYELDRYDEGSAEHLQKFVQLGMAGTLILAAFIGCLGAFLGSIKVLVVHLTLLGILIVSHIWKLSHYNENKQLDATEVYVMALWMEELVHQGAIQQLQREFECCGNKGFGDYTSLNIKVPSSCFHTRDGLHALHPYAEGCMAAVKRAFLQIYRYEKWAHCGLIGYELVGIILGITLCCQLTNKTRRYAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTB8</accession>
    <name>A0A6I8UTB8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein G12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4803788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360450.3">
        <property type="nucleotide sequence ID" value="XM_001360413.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTB8"/>
    <dbReference type="KEGG" id="dpo:4803788"/>
    <dbReference type="InParanoid" id="A0A6I8UTB8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010629">
        <property type="entry name" value="Ins_allergen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21163:SF0">
        <property type="entry name" value="GH08205P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21163">
        <property type="entry name" value="PROTEIN G12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06757">
        <property type="entry name" value="Ins_allergen_rp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026232640" evidence="1">
        <location>
            <begin position="26"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360450.3"/>
        </source>
    </evidence>
    <sequence length="215" mass="24585" checksum="B6CD4319B66CF27C" modified="2020-08-12" version="1">MACLGHAPIAVILLAVVFSCAPTLADLRGDLRDFVAIAPRRRIVYIAARYYIFDPRFRQAVGFVRSDEFLKTWQSVRAAPDVAALIDYLNEYGSGYDVTSLVDSLPQRLRAYQLSRTVPVEMMLHRDLTTFLREAVQTLPRAKVYALMSQKVRQGGDFAKFYKALRDKQFKILVDRARNSADLQAPLKRLHEKNINVDEILQILFEIINWGPQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTC6</accession>
    <name>A0A6I8UTC6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Major facilitator superfamily domain-containing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">jef</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360615.2">
        <property type="nucleotide sequence ID" value="XM_001360578.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234196.1">
        <property type="nucleotide sequence ID" value="XM_033378305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTC6"/>
    <dbReference type="KEGG" id="dpo:4803980"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17335">
        <property type="entry name" value="MFS_MFSD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="562"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="655"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="8">
        <location>
            <begin position="111"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="691"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
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        <location>
            <begin position="713"/>
            <end position="722"/>
        </location>
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        <location>
            <begin position="723"/>
            <end position="737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
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        <source>
            <dbReference type="RefSeq" id="XP_001360615.2"/>
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        <source>
            <dbReference type="RefSeq" id="XP_033234196.1"/>
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    <sequence length="751" mass="82086" checksum="0B2E4451D7DFF487" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTE9</accession>
    <name>A0A6I8UTE9_DROPS</name>
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        <submittedName>
            <fullName evidence="6">Fibrinogen-like protein 1 isoform X1</fullName>
        </submittedName>
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        <name evidence="6" type="primary">LOC4804004</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="FI21813P1-RELATED"/>
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    <dbReference type="PANTHER" id="PTHR19143">
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
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            <begin position="24"/>
            <end position="41"/>
        </location>
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            <begin position="224"/>
            <end position="438"/>
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    </feature>
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            <begin position="110"/>
            <end position="193"/>
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        <source>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001360635.4"/>
        </source>
    </evidence>
    <sequence length="457" mass="52921" checksum="686D636B3BDE88C7" modified="2020-08-12" version="1">MHFVCDLSLILIAMCTVRIIRLTMENYFVLLLILLAVPWSFEATPKLKPTKAAIIDLKNKTEVVPSSSMPIANVTAVHEKLAEVHIDQHKTIRISKGDLDDLLDVYMGKIAESAATIKDKENEINKLQTKEHTDDDLLKRFENLTEICNAQNATYTAKLKEKADQIKELEQKVKVYETRLKRKQHVLTDLRKQNTSSAALIDHLRAKVVYFEGKFREKKDDLLADWEAATTTCMPYGMTSGIHLIHLPGFLPFLVSCEGLTTAGPGWMAIQRRLDGSVNFYRNWDQYRKGFGKLNGEFFIGLEKLHRLTSSQPHELYISFRRFNGESSYAHYDDFVIGSEEEGYELKLLGHYQGNSSDALRTHDKMKFSTFDRDNDEFTHMNCAEYHHGAWWYDFCSRSNLNGKYFKAEVDNVQGIFWEQWYSFRSLRSVQMLIRPKSQMELNDLPRTKRTVPLKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTK9</accession>
    <name>A0A6I8UTK9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4804117</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001360729.2">
        <property type="nucleotide sequence ID" value="XM_001360692.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTK9"/>
    <dbReference type="FunCoup" id="A0A6I8UTK9">
        <property type="interactions" value="1702"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804117"/>
    <dbReference type="InParanoid" id="A0A6I8UTK9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000679">
        <property type="entry name" value="Thymidylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="10">
        <location>
            <begin position="12"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001360729.2"/>
        </source>
    </evidence>
    <sequence length="211" mass="23956" checksum="34F68734B7E52EA2" modified="2020-08-12" version="1">MSVGKRGALFVFEGCDRCGKTTQSRLLAEFLKSKGIPTLSMNFPERESSTGQVINSYLTNSNDLPDEVIHLMFSANRWEYMNKIRKELLAGTTLIVDRYSYSGVAYTAAKGLDFDWCYAPERGLIKPDAVFYLKTASDNLLSRGKYGEERYEKLEFQQKVAEVFNRICEQESSYWHQIDANQSVAEIHTQISGIAEKLQQQVDGQPLNKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTZ2</accession>
    <name>A0A6I8UTZ2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">gek</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">MV-25-SWS-2005</strain>
            <tissue evidence="25">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001360900.3">
        <property type="nucleotide sequence ID" value="XM_001360863.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UTZ2">
        <property type="interactions" value="747"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804322"/>
    <dbReference type="InParanoid" id="A0A6I8UTZ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20809">
        <property type="entry name" value="C1_MRCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01243">
        <property type="entry name" value="PH_MRCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05597">
        <property type="entry name" value="STKc_DMPK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000014">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000032">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001055">
        <property type="entry name" value="Serine/threonine-protein kinase MRCK beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031597">
        <property type="entry name" value="KELK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057529">
        <property type="entry name" value="MRCK/ROCK_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF66">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GENGHIS KHAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15796">
        <property type="entry name" value="KELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25346">
        <property type="entry name" value="PH_MRCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="100"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="23">
        <location>
            <begin position="370"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="999"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="18">
        <location>
            <begin position="1069"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="22">
        <location>
            <begin position="1213"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="domain" description="CRIB" evidence="21">
        <location>
            <begin position="1556"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="439"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="544"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1620"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="646"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="454"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="566"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1625"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_001360900.3"/>
        </source>
    </evidence>
    <sequence length="1646" mass="187687" checksum="ED524449668E39E6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUU3</accession>
    <name>A0A6I8UUU3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Dehydrogenase/reductase SDR family member on chromosome X</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC4804600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361134.1">
        <property type="nucleotide sequence ID" value="XM_001361097.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_004444249.1">
        <property type="nucleotide sequence ID" value="XM_004444192.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUU3"/>
    <dbReference type="GeneID" id="4804600"/>
    <dbReference type="KEGG" id="dpo:4804600"/>
    <dbReference type="OMA" id="HYAARDR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070891">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000720">
        <property type="entry name" value="Carbonyl reductase, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634469" evidence="3">
        <location>
            <begin position="17"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_004444249.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="39647" checksum="6A4A0E2FB0E53995" modified="2020-08-12" version="1">MLIALFWLFFVGLLLCGFLFSKTTKEFPKSWFEWKTEFRYQYLGIVGLIHDAQYAARDRVALYKQPDRIAVITGGNRGIGLRIVEKLLACDMTVVMGVRDPKSAEAAVGAIVDLKATKGKLICEQLDVGDLKSVQAFAQRIKERYTKVDLLLNNAGIMFAPFRLTADGYESHFATNYLGHFLLSHLLLPRLKAAGKEGKNARIVNVSSCVNLIGRINYKDINGNKQYYPGAAYSQSKLAQILYTRHLQTLLDAEKSHVQVNVVHPGIVDTDLFEHSATTSVPMFKKLFFKTPERGSRTVVFAAIDPSIEGQGGTYLSNGGKGPFHPDAKKPAKCEQFFQFSCDLLKIKQYGNGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVY1</accession>
    <name>A0A6I8UVY1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">LOC4805215</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361656.2">
        <property type="nucleotide sequence ID" value="XM_001361619.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040494.1">
        <property type="nucleotide sequence ID" value="XM_015185008.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234098.1">
        <property type="nucleotide sequence ID" value="XM_033378207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVY1"/>
    <dbReference type="GeneID" id="4805215"/>
    <dbReference type="KEGG" id="dpo:4805215"/>
    <dbReference type="OMA" id="DKNHLRC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079150">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF168">
        <property type="entry name" value="GH22922P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634460" description="LRRCT domain-containing protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="7">
        <location>
            <begin position="474"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="625"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="772"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="850"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="883"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="905"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361656.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015040494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033234098.1"/>
        </source>
    </evidence>
    <sequence length="921" mass="104240" checksum="5CBA36AB164C44E8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW66</accession>
    <name>A0A6I8UW66_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein bS16m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">28S ribosomal protein S16, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mRpS16</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361759.2">
        <property type="nucleotide sequence ID" value="XM_001361722.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW66"/>
    <dbReference type="SMR" id="A0A6I8UW66"/>
    <dbReference type="FunCoup" id="A0A6I8UW66">
        <property type="interactions" value="1129"/>
    </dbReference>
    <dbReference type="GeneID" id="4805344"/>
    <dbReference type="KEGG" id="dpo:4805344"/>
    <dbReference type="InParanoid" id="A0A6I8UW66"/>
    <dbReference type="OMA" id="PNDYNER"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080990">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1320.10:FF:000004">
        <property type="entry name" value="28S ribosomal protein S16, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_bS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_bS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_bS16_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919:SF20">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="Ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361759.2"/>
        </source>
    </evidence>
    <sequence length="135" mass="15067" checksum="58EA55A337A044B0" modified="2020-08-12" version="1">MSLSPASGIGRFYKHSAKIIRFVRLGCTNRPFYHIVVMERRKNQHQPVIEQVGSYDPLPNDFNERLVALNTERIRFWLGKGAHISDPAAELLGVAGLLPIHPRTYMTAWRNRRKLAEEQAEAAAAGQAESASASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWI5</accession>
    <name>A0A6I8UWI5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Solute carrier family 25 member 35-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4805289</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361711.3">
        <property type="nucleotide sequence ID" value="XM_001361674.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWI5"/>
    <dbReference type="FunCoup" id="A0A6I8UWI5">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="4805289"/>
    <dbReference type="KEGG" id="dpo:4805289"/>
    <dbReference type="InParanoid" id="A0A6I8UWI5"/>
    <dbReference type="OMA" id="GWVHNKK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074916">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051508">
        <property type="entry name" value="Mito_Carrier_Antiporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928">
        <property type="entry name" value="RE38146P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928:SF1">
        <property type="entry name" value="RE38146P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="106"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="208"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001361711.3"/>
        </source>
    </evidence>
    <sequence length="307" mass="33749" checksum="4BCC1EAEE84BDE9F" modified="2020-08-12" version="1">MMATFTSDFVLGGVAAMGAGLVTNPVEVIKIRMQLQGELAARGSHAQPYKNVLQAFVAVAKNDGILGLQKGLAPALCFQFIINSIRLSTYTRAVEKGWVHNKKGEISFLRGMFWGALGGVVGSYCASPFFLIKTQLQAQAAKQIAVGYQHSHTSMTNAIRQIYRKSGFFGLWRGSMASVIRTTVASSVQISTFGKAKSLLKDNDIITQPTCLSFFSGLAAGSFVSVAVTPFDVITTRLYNQGVDSHGRGLYYKGWLDCVAKILRSEGVYGVYKGFWPIYLRMAPYSTLVLLFFEELIAVREKYDLHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWJ0</accession>
    <name>A0A6I8UWJ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translation initiation factor IF-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4805616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361990.2">
        <property type="nucleotide sequence ID" value="XM_001361953.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWJ0"/>
    <dbReference type="GeneID" id="4805616"/>
    <dbReference type="KEGG" id="dpo:4805616"/>
    <dbReference type="InParanoid" id="A0A6I8UWJ0"/>
    <dbReference type="OMA" id="MASNKEY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072418">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361990.2"/>
        </source>
    </evidence>
    <sequence length="127" mass="14355" checksum="D7B32B8F92B87A01" modified="2020-08-12" version="1">MGCSSSKSDPTIDDTKGPVSAKSNRMASNKEYPASEAFTIPLDTEQQPEMPLNETLRQPPKRIQQLMQEAAHSEPPTLEELEDKQQKAEQRRQELMQQKLEAIQKNVQILMRGHSDGDGHGEERNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWS0</accession>
    <name>A0A6I8UWS0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Extensin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Twdlbeta</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361818.1">
        <property type="nucleotide sequence ID" value="XM_001361781.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWS0"/>
    <dbReference type="FunCoup" id="A0A6I8UWS0">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="GeneID" id="4805413"/>
    <dbReference type="KEGG" id="dpo:4805413"/>
    <dbReference type="InParanoid" id="A0A6I8UWS0"/>
    <dbReference type="OMA" id="QKEYGPP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF13">
        <property type="entry name" value="TWEEDLEBETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026094291" evidence="2">
        <location>
            <begin position="20"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="3">
        <location>
            <begin position="69"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361818.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="21373" checksum="915461949C56988A" modified="2020-08-12" version="1">MKPQIVLLFLAASLALALARPEPPRPRYGAPPPPAPQQEYGPPPPAQPLPVYGPPAAFYGPPAAPAAEAIVTKNVYVHVPPEEPEFYPAAAPIQTAVPKKHYKIIFIKAPNPPTPVRQVLPPPVQDEHKTLVYVLVKKPEEQLPVILPSPAPTEPSKPEVYFIKYKQQQKPAPQYGPPAAPAEEYGAPPAPRGLEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX21</accession>
    <name>A0A6I8UX21_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lysine-specific demethylase 7B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132321.2">
        <property type="nucleotide sequence ID" value="XM_002132285.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX21"/>
    <dbReference type="KEGG" id="dpo:6903442"/>
    <dbReference type="InParanoid" id="A0A6I8UX21"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132321.2"/>
        </source>
    </evidence>
    <sequence length="105" mass="12239" checksum="351B0D9109CB7E10" modified="2020-08-12" version="1">MCIQNCDNIKICDSNGSHHHHHQHHHHHHHRDAQDIVAVVEVVEGGENNVIHTSDPHYENFKSKLQLQSELNRKRQKSVKVKAPKLSNFKILMCCCYFIVIVMMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXU9</accession>
    <name>A0A6I8UXU9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Secretion-regulating guanine nucleotide exchange factor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6903205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132905.2">
        <property type="nucleotide sequence ID" value="XM_002132869.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXU9"/>
    <dbReference type="FunCoup" id="A0A6I8UXU9">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903205"/>
    <dbReference type="InParanoid" id="A0A6I8UXU9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXU9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF8">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC2-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="2"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="56"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="111"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="272"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="327"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002132905.2"/>
        </source>
    </evidence>
    <sequence length="379" mass="41739" checksum="94DAC31114BBC596" modified="2020-08-12" version="1">MEVFAWGANSHGQLGLGFESELCMTPQRLTRHSFAPQLVRCIRGGGGHVLVLDTNGRVHACGWNNRGQLGLDSTEECHNEFQMIPSEFFEEVPIETVSCGWDISGGITLTKRLFVWGSNAFQQLGICQRGFMAVRRPLPVRLPKEEPALAISFGLRHCAVLTQDHKIYVFGRLRVMDPPPIELDITATTLHRCNTMKIQVHNPNELRIVALSSGQHHMLLKCVDLSQEGGSTKRILSLGDNKFGQSNAFLFEEDVRQLAVGWTHNAALLRNNEILMWGRNCYGQLGTGSISAQQAVPQPLSLRLPDGQTPARVHMGAEHGLLRTTAGEIYTWGWNEHGNCGNNSTENVYSPTLVEIPGRVKLAGAGSGFCYAISESSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY37</accession>
    <name>A0A6I8UY37_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Xenotropic and polytropic retrovirus receptor 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6902624</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133150.2">
        <property type="nucleotide sequence ID" value="XM_002133114.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY37"/>
    <dbReference type="KEGG" id="dpo:6902624"/>
    <dbReference type="InParanoid" id="A0A6I8UY37"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14477">
        <property type="entry name" value="SPX_XPR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF127">
        <property type="entry name" value="LD30826P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="7">
        <location>
            <begin position="413"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002133150.2"/>
        </source>
    </evidence>
    <sequence length="617" mass="73155" checksum="262D2947CD089D11" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V185</accession>
    <name>A0A6I8V185_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nuclear pore complex protein Nup133-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6899993</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin Nup133 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135359.2">
        <property type="nucleotide sequence ID" value="XM_002135323.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V185"/>
    <dbReference type="FunCoup" id="A0A6I8V185">
        <property type="interactions" value="2482"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899993"/>
    <dbReference type="InParanoid" id="A0A6I8V185"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1380:FF:000002">
        <property type="entry name" value="Nup133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007187">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037624">
        <property type="entry name" value="Nup133-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13405">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13405:SF11">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03177">
        <property type="entry name" value="Nucleoporin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="10">
        <location>
            <begin position="58"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like C-terminal" evidence="9">
        <location>
            <begin position="808"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002135359.2"/>
        </source>
    </evidence>
    <sequence length="1161" mass="131011" checksum="159894452678F426" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1Z7</accession>
    <name>A0A6I8V1Z7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6896677</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002136892.2">
        <property type="nucleotide sequence ID" value="XM_002136856.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1Z7"/>
    <dbReference type="KEGG" id="dpo:6896677"/>
    <dbReference type="InParanoid" id="A0A6I8V1Z7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="384"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002136892.2"/>
        </source>
    </evidence>
    <sequence length="442" mass="51353" checksum="805461595A78DF83" modified="2020-08-12" version="1">MERRVRSSGRESRQGKGASAKESNKKWKDCGHGKTLHISEYGPFVVRTCPKKPLSEYKLKRARYPMMLGMSSQKISKSKISLALEKPPMLATSSQSELDLFKLDTMIDRIWSDLVDKHARLIKAGKRPEEVVDSLKSRYIQQTQRVLLNAAGSITKPAVGRTRMSNPAYVTHRQLVEQQMEWLSTKKVAKKLYLLKELLEQKRMPQGKQDRRMLEEYGDSSQHYNGLLMVFNKEIPVDQRNHQMDTISKTFKTIMDQLDDYASRSRMQSSNGSILSKDSDGQDPQLEVKTLITLENIEATRAEWMQYFADRPRTPFEQKLSAIRLQRKEALIAEKMELKRIKMEKRKEFLDHRPLTELYNNPRLTNRMRQVLAERAEQARRQAAHSQSQSPSESVHVHPKNEPRRPRSSAKRLSISDVVLKTMEERGSCCKCQWIPRWIKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2E2</accession>
    <name>A0A6I8V2E2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acyl-aliphatic-L-amino acid amidohydrolase</fullName>
            <ecNumber evidence="3">3.5.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-acyl-L-amino-acid amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6897000</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.14" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002137186.2">
        <property type="nucleotide sequence ID" value="XM_002137150.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2E2"/>
    <dbReference type="KEGG" id="dpo:6897000"/>
    <dbReference type="InParanoid" id="A0A6I8V2E2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V2E2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004046">
        <property type="term" value="F:aminoacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05646">
        <property type="entry name" value="M20_AcylaseI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000019">
        <property type="entry name" value="Aminoacylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.900:FF:000001">
        <property type="entry name" value="Aminoacylase-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000005">
        <property type="entry name" value="Putative Aminoacylase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052083">
        <property type="entry name" value="Aminoacylase-1_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010159">
        <property type="entry name" value="N-acyl_aa_amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01880">
        <property type="entry name" value="Ac-peptdase-euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45892">
        <property type="entry name" value="AMINOACYLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45892:SF1">
        <property type="entry name" value="AMINOACYLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036696">
        <property type="entry name" value="ACY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="11">
        <location>
            <begin position="189"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036696-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036696-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137186.2"/>
        </source>
    </evidence>
    <sequence length="401" mass="45176" checksum="145FBB38355EB1A8" modified="2020-08-12" version="1">MSASDWESNEELQYFREYLRIPSVHPNPNYEPCVEFLRRQAKQLELPIKVYYPANEHNPVVVLTWQGLEPELPSVLLNSHMDVVPVFPENWTHPPFGAEIDDEGRIFARGTQDMKCVGMQYLAAIRALKRNGARFKRTIHISFVADEEMGGRLAMRPFVDSKEFRALNVGFGLDEGIASPTSEIPVFYAERTVRRVLFKIGGTAGHGSLLLPNTAGEKLSYILGKMMEFRKVQFQRLESNPELSIGDVTTVNLTRVEGGVQSNVVPPQLMVCFDCRLALDIDIHEFDANLHKWADEAGGGIELEYEQKRGHIPPTATDETNPFWVAFKEATDHLGLEIKLQVFPGGTDSRYLRNAGISALGFSPMNHTPVLLHDHDEFLHAQTYLKGVQIYEKIISNVASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2G4</accession>
    <name>A0A6I8V2G4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acyl-aliphatic-L-amino acid amidohydrolase</fullName>
            <ecNumber evidence="3">3.5.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-acyl-L-amino-acid amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6896999</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.14" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002137185.2">
        <property type="nucleotide sequence ID" value="XM_002137149.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2G4"/>
    <dbReference type="FunCoup" id="A0A6I8V2G4">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896999"/>
    <dbReference type="InParanoid" id="A0A6I8V2G4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004046">
        <property type="term" value="F:aminoacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05646">
        <property type="entry name" value="M20_AcylaseI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000019">
        <property type="entry name" value="Aminoacylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.900:FF:000001">
        <property type="entry name" value="Aminoacylase-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000005">
        <property type="entry name" value="Putative Aminoacylase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052083">
        <property type="entry name" value="Aminoacylase-1_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010159">
        <property type="entry name" value="N-acyl_aa_amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01880">
        <property type="entry name" value="Ac-peptdase-euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45892">
        <property type="entry name" value="AMINOACYLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45892:SF1">
        <property type="entry name" value="AMINOACYLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036696">
        <property type="entry name" value="ACY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="11">
        <location>
            <begin position="188"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036696-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036696-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137185.2"/>
        </source>
    </evidence>
    <sequence length="406" mass="45657" checksum="5CE1D6F6069D91C2" modified="2020-08-12" version="1">MSASKWESDEEIQFFREYLRIPSVHPNPNYEPCLEFLDRQAKQLELPMKVYYPADEQNPVVVLTWQGLKPELPSVLLNSHMDVVPVFPENWTHPPFGAEIDEEGRIFARGTQDMKCVGMQYLAAIRALKRSGARFRRTIHISFVADEEMGGKLGMRPFVHTDDFRALNVGFGMDEGLASPTAEFPVFYAERSVWRVYFHISGTSGHGSLLLSNTAGEKLNYIVGKMMAYRKVQVQRLENNPELCIGDVTTINLTKVEGGVQSNVVPPLLMVCFDCRLALDVDHLEFEATLEKWCAEAGGGIELTYEQKQPRVLPTPIDASNHFWVAFKKATDNLGLAIKPQIFTGGTDSRYLRQVGIPALGFSPMNNTPVLLHDHDEFLQADTYLRGVQIYQKIISNIANVGCVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V359</accession>
    <name>A0A6I8V359_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Pp1alpha-96A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="RefSeq" id="XP_002137627.1">
        <property type="nucleotide sequence ID" value="XM_002137591.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V359"/>
    <dbReference type="SMR" id="A0A6I8V359"/>
    <dbReference type="FunCoup" id="A0A6I8V359">
        <property type="interactions" value="2469"/>
    </dbReference>
    <dbReference type="GeneID" id="6897478"/>
    <dbReference type="KEGG" id="dpo:6897478"/>
    <dbReference type="InParanoid" id="A0A6I8V359"/>
    <dbReference type="OMA" id="SIFICRG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247808">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="11">
        <location>
            <begin position="119"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137627.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="37386" checksum="90AED1D0567D84FC" modified="2020-08-12" version="1">MSDIMNIDSIISRLLEVRGARPGKNVQLSESEIRSLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYSENFFLLRGNHECASINRIYGFYDECKRRYTIKLWKTFTDCFNCLPVAAIVDEKIFCCHGGLSPDLSSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDTMGWGENDRGVSFTFGAEVVGKFLQKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDDTLMCSFQILKPADKRRFVYPNFGSSGRPLTPPRGANNKNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5K1</accession>
    <name>A0A6I8V5K1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Chorion peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">cd</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_003736536.3">
        <property type="nucleotide sequence ID" value="XM_003736488.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015181806.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8V5K1"/>
    <dbReference type="KEGG" id="dpo:4801534"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022412">
        <property type="term" value="P:cellular process involved in reproduction in multicellular organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09823">
        <property type="entry name" value="peroxinectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.640.10:FF:000003">
        <property type="entry name" value="chorion peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF141">
        <property type="entry name" value="CARDINAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0349">Heme</keyword>
    <keyword evidence="7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 11 12" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="545"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_003736536.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015037292.2"/>
        </source>
    </evidence>
    <sequence length="832" mass="93221" checksum="D6875D451C2E0D10" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6I6</accession>
    <name>A0A6I8V6I6_DROPS</name>
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        <submittedName>
            <fullName evidence="12">Extracellular serine/threonine protein CG31145 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6896811</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736267.3">
        <property type="nucleotide sequence ID" value="XM_003736219.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8V6I6"/>
    <dbReference type="FunCoup" id="A0A6I8V6I6">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896811"/>
    <dbReference type="InParanoid" id="A0A6I8V6I6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V6I6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10314">
        <property type="entry name" value="FAM20_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF22">
        <property type="entry name" value="EXTRACELLULAR SERINE_THREONINE PROTEIN CG31145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM20 C-terminal" evidence="10">
        <location>
            <begin position="856"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="268"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="623"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="659"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="632"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="660"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="964"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="773"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="789"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="810"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="810"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="892"/>
            <end position="895"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="969"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="984"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="984"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_003736267.3"/>
        </source>
    </evidence>
    <sequence length="1084" mass="120490" checksum="A009CB5808AB5A99" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VB47</accession>
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        <submittedName>
            <fullName evidence="4 5">Dynein assembly factor 5, axonemal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">Dnaaf5</name>
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    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037491.2">
        <property type="nucleotide sequence ID" value="XM_015182005.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232574.1">
        <property type="nucleotide sequence ID" value="XM_033376683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VB47"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cilium movement"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:inner dynein arm assembly"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:outer dynein arm assembly"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 5"/>
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        <property type="entry name" value="TPR_DNAAF5"/>
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    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein axonemal assembly factor 5 TPR repeats" evidence="2">
        <location>
            <begin position="20"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein axonemal assembly factor 5 HEAT-repeat" evidence="1">
        <location>
            <begin position="321"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015037491.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232574.1"/>
        </source>
    </evidence>
    <sequence length="855" mass="97861" checksum="9EBC5D80CCB17223" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VCL3</accession>
    <name>A0A6I8VCL3_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4802637</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_015182478.2"/>
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    <dbReference type="InParanoid" id="A0A6I8VCL3"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:cell adhesion molecule binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="5"/>
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        <property type="entry name" value="CD80_C2-set"/>
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        <property type="entry name" value="Cell_adhesion_signaling"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="2 7" id="KW-0472">Membrane</keyword>
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    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="585"/>
            <end position="608"/>
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    </feature>
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            <end position="163"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="8">
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            <end position="283"/>
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    <feature type="domain" description="Ig-like" evidence="8">
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            <end position="463"/>
        </location>
    </feature>
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            <end position="26"/>
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    </feature>
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            <end position="717"/>
        </location>
    </feature>
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        <location>
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            <end position="885"/>
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    </feature>
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        <location>
            <begin position="995"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="1100"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
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            <end position="637"/>
        </location>
    </feature>
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        <location>
            <begin position="638"/>
            <end position="650"/>
        </location>
    </feature>
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        <location>
            <begin position="660"/>
            <end position="696"/>
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    </feature>
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    </feature>
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    </feature>
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        <location>
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            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1100"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015037964.2"/>
        </source>
    </evidence>
    <sequence length="1149" mass="128547" checksum="134844419E23153B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VE41</accession>
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        <submittedName>
            <fullName evidence="7 8">Serine/threonine-protein kinase meng-po isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">LOC4803782</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <strain evidence="6">MV2-25</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004674">
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <location>
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            <begin position="398"/>
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            <position position="55"/>
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        <ligand>
            <name>ATP</name>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015039360.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033234787.1"/>
        </source>
    </evidence>
    <sequence length="588" mass="66482" checksum="A35EDF515D2022D2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VEC4</accession>
    <name>A0A6I8VEC4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Sik3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_015039326.2">
        <property type="nucleotide sequence ID" value="XM_015183840.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VEC4">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VEC4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14338">
        <property type="entry name" value="UBA_SIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000156">
        <property type="entry name" value="Serine/threonine-protein kinase SIK3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055511">
        <property type="entry name" value="DUF7084"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057380">
        <property type="entry name" value="UBA_SIK1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF42">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23311">
        <property type="entry name" value="DUF7084"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23312">
        <property type="entry name" value="UBA_SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="50"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="17">
        <location>
            <begin position="1436"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="673"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1258"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1380"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1271"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1290"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1307"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1319"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1403"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015039326.2"/>
        </source>
    </evidence>
    <sequence length="1494" mass="160445" checksum="0B4E9DBEFA86D63B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFA0</accession>
    <name>A0A6I8VFA0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039931.2">
        <property type="nucleotide sequence ID" value="XM_015184445.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFA0"/>
    <dbReference type="KEGG" id="dpo:26532794"/>
    <dbReference type="InParanoid" id="A0A6I8VFA0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026049148" evidence="1">
        <location>
            <begin position="26"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015039931.2"/>
        </source>
    </evidence>
    <sequence length="152" mass="17123" checksum="B61D9898295CBA8D" modified="2020-08-12" version="1">MCFVKNASLWPLLPLFCLVVRSSSATPALQEEALQQIPTLNLTQVQLIAGNASLRCSLIPELCDWQLHLYSGHHFSVPLSFAESTVSTTTLSPMEVVAASTPTEQVFSLAPLRLVAQIEPKPRREKRGKRKLRRRSTFGILSKKQFVWLFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFG6</accession>
    <name>A0A6I8VFG6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Fatty acid 2-hydroxylase</fullName>
            <ecNumber evidence="18">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25 26" type="primary">Fa2h</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">MV-25-SWS-2005</strain>
            <strain evidence="24">MV2-25</strain>
            <tissue evidence="25">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26">MV-25-SWS-2005</strain>
            <tissue evidence="26">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes stereospecific hydroxylation of free fatty acids at the C-2 position to produce (R)-2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis. Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath. Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier. Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis. Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 19">Binds 2 Zn(2+) ions per subunit that likely form a catalytic dimetal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the sterol desaturase family. SCS7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="18"/>
    <dbReference type="RefSeq" id="XP_002138439.3">
        <property type="nucleotide sequence ID" value="XM_002138403.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039657.2">
        <property type="nucleotide sequence ID" value="XM_015184171.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFG6"/>
    <dbReference type="KEGG" id="dpo:6898392"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005149">
        <property type="entry name" value="IPC-B_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17 18" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="10 18" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 20" id="KW-0349">Heme</keyword>
    <keyword evidence="14 18 20" id="KW-0408">Iron</keyword>
    <keyword evidence="5 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 19" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="23">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="71"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_002138439.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_015039657.2"/>
        </source>
    </evidence>
    <sequence length="350" mass="41650" checksum="99EC21A4D5622485" modified="2020-08-12" version="1">MDTPKGSDAGFIVKYRQEYYDLSEFMHKHPGGINTLNGLNQADMTARFMKAPPHSDAAMYLMKEYKVKSEDPRIPMTKPRHQARRIDNGQDSQERSNESEDKNNNQLDESMEHLVDWSKAMLPQIANITKCYDEWVHKPVDRPLRLFGPWYLEMLTKTPWWLVPIFWIPVIIRFAWEDIHTSWNDKSQLACVTGYFLFGVLLWTFLEYTLHRWVFHIKLKCNSGPWICTFHFMIHGLHHKVPFDPMRLVFPPLPGAILATLIYYPLSYFLIQPRVVLSGALAGYLCYDMMHYYLHYGNPSFRHIVHMKRYHYQHHFTHQDLGYGISSPLWDVVFKTRIHLRKLKYQLRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIU9</accession>
    <name>A0A6I8VIU9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4814245</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042255.2">
        <property type="nucleotide sequence ID" value="XM_015186769.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VIU9"/>
    <dbReference type="FunCoup" id="A0A6I8VIU9">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VIU9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026141432" evidence="3">
        <location>
            <begin position="25"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="20"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="171"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015042255.2"/>
        </source>
    </evidence>
    <sequence length="454" mass="50529" checksum="7A76C8DCA4609E9C" modified="2020-08-12" version="1">MSSTSSSVLTLSSFNILFFTQVLTVLSSTIKYNEYATDKGGNVSIPCIAQGNIMWVKEHGSNSTIVQTGRVLVLRNVSISDAGIYVCFATTTTTRATTTASPATTTSPRREKEEEQQLLPEEDTATTTAQPKRQELGQKPDQDQDQGQEEEGEEELAYQAYQRTKLTVRTVPGPVTQLYFKASTILGFLIWRFNKTQSGGYPVRSFTAEFRNVSYSQPPANASFEHAWTRMDPINIAPNVRQMEVYRLAPNTTYEFRIWANNELGSGVVVTTNVTTLPETKEEDLIRLIKPDLDNFDPRIWIVAVSIVLGTLVILAIGLCIVLSKECYQASQMELQDGWDSIELIPNIILNPGFSESDGGGGAAGGDAENDAEAEAAAEAEEEARVRFTRTIIFGQKPRPSRLHPPGHPQYRPPPPVVVFDDDDEDYEEEHEPTMERFKRKVSVFFTGPTIKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VND9</accession>
    <name>A0A6I8VND9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897962</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232590.1">
        <property type="nucleotide sequence ID" value="XM_033376699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VND9"/>
    <dbReference type="InParanoid" id="A0A6I8VND9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VND9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056677">
        <property type="entry name" value="DUF7775"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41152">
        <property type="entry name" value="AT26438P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41152:SF8">
        <property type="entry name" value="AT26438P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24985">
        <property type="entry name" value="DUF7775"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7775" evidence="2">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033232590.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="23974" checksum="D61E893A20558B39" modified="2020-08-12" version="1">MLRPVLFLFYTVETVVNIILMGYHIRGFMSLDLSFLPLKDQTTYYIYTVNFYVFTGITAFASISASTGHKAVLLEEFLRSLAGCILFLIMSLMTLKDVENDFHMMYLGMGDPKLPEKPVHPFFAYLHAQAVCSLVGCIIYLMHCLIVIDVMLSHEEADYDLEVYDASDDSDEDYIPVRLYFCCAYVQERLERYRWFREFSSGGRMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNS9</accession>
    <name>A0A6I8VNS9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Uncharacterized protein Dh44 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">Dh44</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137926.2">
        <property type="nucleotide sequence ID" value="XM_002137890.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232716.1">
        <property type="nucleotide sequence ID" value="XM_033376825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNS9"/>
    <dbReference type="KEGG" id="dpo:6897821"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VNS9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018446">
        <property type="entry name" value="Corticotropin-releasing_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000187">
        <property type="entry name" value="CRF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00473">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00039">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00511">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0372">Hormone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634539" evidence="5">
        <location>
            <begin position="25"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Corticotropin-releasing factor" evidence="6">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137926.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033232716.1"/>
        </source>
    </evidence>
    <sequence length="310" mass="34934" checksum="CEF8A81F627EDA33" modified="2020-08-12" version="1">MMKATAWFCPVLLTLLCATRLVCTAQRGTGTGSGAEQQPAGNSHTNGYPLDYLDARNTQDDFLLAKRNKPSLSIVNPLDVLRQRLLLEIARRQMKENTRQVELNRAILKNVGKRVYLRPAAAEAILSRPYSQQLERALARSNKRQQKMHWTNFPSPLQWSYAMPQSPDTSQFQSQSQSQKSQAQKLFNYAKKSLNGAGVGPGLGMSVAAKHPADGNEVANEKYHENHGDRNAAMASKNATIYVADTDEVDDENENDDNEEEESRVEQAFEEDKPNLEPHGMAAGVDNTIEYIPWNLLYRYLPQRKHYNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNT7</accession>
    <name>A0A6I8VNT7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117183254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232424.1">
        <property type="nucleotide sequence ID" value="XM_033376533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNT7"/>
    <dbReference type="KEGG" id="dpo:117183254"/>
    <dbReference type="InParanoid" id="A0A6I8VNT7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232424.1"/>
        </source>
    </evidence>
    <sequence length="107" mass="10421" checksum="F855CE901C824136" modified="2020-08-12" version="1">MGLGLGHGHGHGLSVGATSPTTSVASASLIGAHCSAVGSGAMAAAPLGNICNTLPHAPSSNVPLNRNLVMLRNHLRKNTSGSSNSGGGGGGSGGVQQLLQDQDDKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQC0</accession>
    <name>A0A6I8VQC0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF243 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6897146</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232929.1">
        <property type="nucleotide sequence ID" value="XM_033377038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQC0"/>
    <dbReference type="FunCoup" id="A0A6I8VQC0">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897146"/>
    <dbReference type="InParanoid" id="A0A6I8VQC0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026013669" description="DUF243 domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="3">
        <location>
            <begin position="87"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232929.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="26608" checksum="CA58D9A55E9F8D8E" modified="2020-08-12" version="1">MRAFIVLCLVALASADKLGYNYQPVAHSPSGLSFQPSGLASNEAPSFEPSGLASNDAPSFAAGPSAPIDGPVGASSSGSPNYAAPQAELEKEFFTYTADEGDFYDPAASEKVSSALNKALRVVFIKGPENRGLENAALALAKQAAQQETAIYVLNKQADIGDLANKLNAIRSNNNNKPEVHFVKYRTPEDAANAQRAIQGQYDQLGGSSQSTDGGVASSLNFASQPAPAHENIGGSASAPGASYLPANIFRRLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTH3</accession>
    <name>A0A6I8VTH3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Polyadenylate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">LOC4805590</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361971.1">
        <property type="nucleotide sequence ID" value="XM_001361934.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234367.1">
        <property type="nucleotide sequence ID" value="XM_033378476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTH3"/>
    <dbReference type="SMR" id="A0A6I8VTH3"/>
    <dbReference type="GeneID" id="4805590"/>
    <dbReference type="KEGG" id="dpo:4805590"/>
    <dbReference type="OMA" id="QPAAIMF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078306">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000729">
        <property type="entry name" value="Blast:Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="102"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="192"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033234367.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="32810" checksum="962F303AD7A39F7D" modified="2020-08-12" version="1">MFVNSMTFRGNPANYHQQQPALNHHTLAAAHHQQQLHHHAAAGHLGHVGGGHAASNHLAAAAVLGRHGHSSVTSGHTTSSSHSAGALASGSTGSSTSSPDSGKIYIKNLERSIDNKAVYDTFSVFGNILNCNVAKDEDGNSRGYGFVHFDSEEAARAAIEKVNGMLCNNQKVHVVKFIPRRDREQEKATHFKNLYVKNLSEEFTEQHLREMFEPYGRITSHKLMLDEEGRSRRFGFVAFENPQSALAAVIGLHGKQLGDNKFLYVARALSKAERQQEINRKLEERKRQKAGQIFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VV85</accession>
    <name>A0A6I8VV85_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine-rich cell wall structural protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234853.1">
        <property type="nucleotide sequence ID" value="XM_033378962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VV85"/>
    <dbReference type="FunCoup" id="A0A6I8VV85">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898624"/>
    <dbReference type="InParanoid" id="A0A6I8VV85"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245683">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033234853.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="20668" checksum="AD35FA32F93496BE" modified="2020-08-12" version="1">MLMGLPYGRFAPHHLRHHCHNIVKVLVYTICTFVCIKNQVESCLASHNVSDHATTMRFDLQLLLLSICSWTISAHSLVGAGVGGVAGVAGVGGGFVQQSPYIQQVPVVQQVPIVQQIPIVQQPQPQPQALGLAGGGLIGGGIGGGVGAAAGFGRYRESYRVRGRGLGGGFRARYDKKIGGGFAGFGAAAGAGAIGGAGGGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVR6</accession>
    <name>A0A6I8VVR6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein toll-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC26532899</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235172.1">
        <property type="nucleotide sequence ID" value="XM_033379281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VVR6"/>
    <dbReference type="FunCoup" id="A0A6I8VVR6">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532899"/>
    <dbReference type="InParanoid" id="A0A6I8VVR6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000727">
        <property type="entry name" value="Toll-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF530">
        <property type="entry name" value="MSTPROX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026202318" evidence="10">
        <location>
            <begin position="21"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="797"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="11">
        <location>
            <begin position="847"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033235172.1"/>
        </source>
    </evidence>
    <sequence length="1005" mass="117021" checksum="1188AF50EC7F5363" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXP1</accession>
    <name>A0A6I8VXP1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin domain-containing protein 5 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6903207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235748.1">
        <property type="nucleotide sequence ID" value="XM_033379857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXP1"/>
    <dbReference type="KEGG" id="dpo:6903207"/>
    <dbReference type="InParanoid" id="A0A6I8VXP1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="263"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235748.1"/>
        </source>
    </evidence>
    <sequence length="385" mass="43565" checksum="B03E02513CFA0156" modified="2020-08-12" version="1">MAALKDINVDDDVVGDDDDDDPVSEACKHDEVLQLNTNNFNLLTQTGKFFIMIFKPPLSYMADQAKLKWVKLARELNPKGTICISDLDCSKSIEICHHLQIRPSPTFLWYENGRKVRAYDAEPDPELEHFKSFVEKVIASNEISVESSASGVEDTDHRTIDWSTDDFETYLKEKNVIVEFYATLCESCNSLYSILWKLMGIQNTASTSLAIGAINCSKYESFCISNNVTMYPTLLYFEKDDGVAKKSYNLSSFMSEKTTEKSVVIKEDAPQNCAPGQVFTLTDKSFDSSIKSMKSFVKFFQPSCPFCTAMKQVWIDLASELRNVTAVCIAELDCTDFKAICKRYHITAVPKLVWIDSNAFRVYNGGGKPLFIEQQSPLYEDKRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W131</accession>
    <name>A0A6I8W131_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein midgut expression 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236424.1">
        <property type="nucleotide sequence ID" value="XM_033380533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W131"/>
    <dbReference type="KEGG" id="dpo:6903103"/>
    <dbReference type="InParanoid" id="A0A6I8W131"/>
    <dbReference type="OMA" id="MANRVYN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247406">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W131">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236424.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="10777" checksum="5FBA459EC9F2B7BC" modified="2020-08-12" version="1">MCVGIIGSALCCCLKLGTKVLFCIACSGIGILIIGGLVIYFVFFHNKSGKKTTTVEDATTVTVAVRAMANRVYNEHLKQHLFGNDMPDEKAEDDLGPLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1L6</accession>
    <name>A0A6I8W1L6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LOW QUALITY PROTEIN: uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC26533170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237178.1">
        <property type="nucleotide sequence ID" value="XM_033381287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W1L6"/>
    <dbReference type="KEGG" id="dpo:26533170"/>
    <dbReference type="InParanoid" id="A0A6I8W1L6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026228702" evidence="2">
        <location>
            <begin position="28"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033237178.1"/>
        </source>
    </evidence>
    <sequence length="410" mass="45818" checksum="C85D3FF6164204F9" modified="2020-08-12" version="1">MHCYRILMKFIQIDVLVILISLKQGNGNYIGTRGREYPSQRNANNNNGVKSEDSSTVVNSGGVRAVGEVFVPKTFSLSAQEPTCEQLRAMWIFSKRQSRAAEITNEIPTYRDPFTYNVWDPLYSNSRVLGSIRMSARERARSPVFGRVVSREPITPQRMTFEQRQRFVDLGSSGIGGPIQARFYGSEGRPQNAGTRRSSKNRYMGVPNGTGASLTGNQNSVAVQGSFQKLKELIWTERAKELTQQRRDEELAARAAALKEIANGQNIQSSYRPQPISPSDSILMDENRSPEGGSYQYSDNNRMSILSGQNLENNKNNKNEHRNRVRATTRRIGNSNFALYNGNGRQAHKEAESMFSGNYATAQSGRGSRIQIPATELFSSDLPDRDHSVIGIPRPTLFDHPTSEKEIGPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W229</accession>
    <name>A0A6I8W229_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BTB/POZ domain-containing protein 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6900052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237410.1">
        <property type="nucleotide sequence ID" value="XM_033381519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W229"/>
    <dbReference type="KEGG" id="dpo:6900052"/>
    <dbReference type="InParanoid" id="A0A6I8W229"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048512">
        <property type="term" value="P:circadian behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052407">
        <property type="entry name" value="BTB_POZ_domain_cont_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033237410.1"/>
        </source>
    </evidence>
    <sequence length="309" mass="35683" checksum="D3B3247D304E0C67" modified="2020-08-12" version="1">MCTQVNEASSPGTTKGTNTDDIDVIDRVELDMGRLCMNDLYSDVEFLVEEHRLPAHRNILAVRSEYFSAMLYEKNLQQNASLSNICKILNAAHRYSMNGLRKKCLNFMDCKASDVLQHASFAMLCKESLEEVLERDTFYAPEVEIFQAVCKWRALFPSEDIKTVISHIRLPLMSVKELLRVVRPTGIFDLDQIMDAMDQIKTTENLPHRSVVLRGENVCKVIRCCQYDYTVFEISKVSLINCISLTVSVGGKWTVRFTHNMEHMYSVGTYDMKPNVRRDIYFEKAFARYIYVTAFNQNEIDSEVIVKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4B3</accession>
    <name>A0A6I8W4B3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6900418</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238181.1">
        <property type="nucleotide sequence ID" value="XM_033382290.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900418"/>
    <dbReference type="InParanoid" id="A0A6I8W4B3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF106">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026249658" description="Chitin-binding type-2 domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="2012"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="95"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="155"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="209"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="312"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="373"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="430"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="572"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="678"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1213"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1274"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1346"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1459"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1529"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1589"/>
            <end position="1643"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1654"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1722"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1797"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1882"/>
            <end position="1938"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="7">
        <location>
            <begin position="1944"/>
            <end position="2000"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033238181.1"/>
        </source>
    </evidence>
    <sequence length="2012" mass="217887" checksum="3A782CB9765CF68F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4Y4</accession>
    <name>A0A6I8W4Y4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4812085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238393.1">
        <property type="nucleotide sequence ID" value="XM_033382502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4Y4"/>
    <dbReference type="KEGG" id="dpo:4812085"/>
    <dbReference type="InParanoid" id="A0A6I8W4Y4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007970">
        <property type="entry name" value="DUF733"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05306">
        <property type="entry name" value="DUF733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033238393.1"/>
        </source>
    </evidence>
    <sequence length="109" mass="13213" checksum="D686AC4713067681" modified="2020-08-12" version="1">MSETARNNPTRKGEETVSYALYLHRQELGRPKRRLMRIAGTKLQLTNELILLQQRRQWAADSPAELVYQQRSALNRESMYRDRLWRNMQRQLEKQHHRQRLNLQQMGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W643</accession>
    <name>A0A6I8W643_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nuclear envelope integral membrane protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC117184588</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238825.1">
        <property type="nucleotide sequence ID" value="XM_033382934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W643"/>
    <dbReference type="FunCoup" id="A0A6I8W643">
        <property type="interactions" value="1294"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184588"/>
    <dbReference type="InParanoid" id="A0A6I8W643"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W643">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019358">
        <property type="entry name" value="NEMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598:SF1">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10225">
        <property type="entry name" value="NEMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026011677" evidence="10">
        <location>
            <begin position="23"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="418"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033238825.1"/>
        </source>
    </evidence>
    <sequence length="450" mass="51672" checksum="9351357999E7F991" modified="2020-08-12" version="1">MFQPRLRCGFISLFTLMAICAAATPHPMTDVVFLAPRTSIDITANWNGPNTLRTYCYPGKPPSLLSLFETVEFLLDIGNDDYTEYRGRTPKEVLDHYNEQRSLFSFTLFSQKRQRIHLSPFKPECIGVSSRQPYNVRLRHAQFDVLRFLQFGLGVLVFCSSRTLAKNSIFYYLAGIVLGICGSLLLIISLTSKLFPRRPMMYGVLIGGWTIGVYILKQLADNMCLILLTYRDYVVWYLIITSLISFLVCYRIGPPKNSRSQNIIMWLLQAVGGGLAYFSSWNSSGVIFLIVSAFAAYYFPQSLVVYGKSLYRRRFPPKRRLLTQEEYYQQTVNETQKSLAELREFVNSPNCNQWNIMSNLRNPIRFASFANGEPHLFDEEIEDYSRTIEESMEAAVQNEAEDYLQCSMHYRPLSNNGFNVSQDHRRRPPMPPPFTGLPTRHATCEHSAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W662</accession>
    <name>A0A6I8W662_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubulin--tyrosine ligase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC117184592</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamyl-[protein] + L-glutamate + ATP = gamma-L-glutamyl-L-glutamyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60144"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143622"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:60145"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238836.1">
        <property type="nucleotide sequence ID" value="XM_033382945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W662"/>
    <dbReference type="FunCoup" id="A0A6I8W662">
        <property type="interactions" value="603"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184592"/>
    <dbReference type="InParanoid" id="A0A6I8W662"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W662">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000009">
        <property type="entry name" value="tubulin polyglutamylase TTLL5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF145">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033238836.1"/>
        </source>
    </evidence>
    <sequence length="862" mass="98437" checksum="47D59C503E256C93" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6I9</accession>
    <name>A0A6I8W6I9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Proton channel OtopLc isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">OtopLc</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the otopetrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238958.1">
        <property type="nucleotide sequence ID" value="XM_033383067.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8W6I9">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815915"/>
    <dbReference type="InParanoid" id="A0A6I8W6I9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015252">
        <property type="term" value="F:proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004878">
        <property type="entry name" value="Otopetrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522">
        <property type="entry name" value="PROTON CHANNEL OTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21522:SF61">
        <property type="entry name" value="PROTON CHANNEL OTOPLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03189">
        <property type="entry name" value="Otopetrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="925"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1007"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1046"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1077"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1173"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1232"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1259"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1304"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1333"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1375"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1404"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="135"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="477"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="763"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="953"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="267"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="512"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="563"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="652"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="663"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="674"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="822"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="955"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033238958.1"/>
        </source>
    </evidence>
    <sequence length="1449" mass="156504" checksum="721D072E48BA58B8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6U0</accession>
    <name>A0A6I8W6U0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">SEC14 domain and spectrin repeat-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">LOC4812963</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354028.3">
        <property type="nucleotide sequence ID" value="XM_001353992.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043421.2">
        <property type="nucleotide sequence ID" value="XM_015187935.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239068.1">
        <property type="nucleotide sequence ID" value="XM_033383177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6U0"/>
    <dbReference type="KEGG" id="dpo:4812963"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W6U0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056804">
        <property type="entry name" value="Spectrin_SESTD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46607">
        <property type="entry name" value="SEC14 DOMAIN AND SPECTRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46607:SF1">
        <property type="entry name" value="SEC14 DOMAIN AND SPECTRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24915">
        <property type="entry name" value="Spectrin_SESTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SESTD1-like spectrin repeats region" evidence="3">
        <location>
            <begin position="421"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="682"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="770"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="842"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1000"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="709"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="887"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1051"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354028.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015043421.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033239068.1"/>
        </source>
    </evidence>
    <sequence length="1086" mass="120754" checksum="7998E21187C95174" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAA9</accession>
    <accession>Q2M001</accession>
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        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">Kap-alpha1</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
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        <property type="nucleotide sequence ID" value="XM_001353586.4"/>
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    <dbReference type="RefSeq" id="XP_033240328.1">
        <property type="nucleotide sequence ID" value="XM_033384437.1"/>
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    <dbReference type="GeneID" id="4813403"/>
    <dbReference type="KEGG" id="dpo:4813403"/>
    <dbReference type="OMA" id="EMIQMLY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081144">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000001">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000009">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
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    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="Arm_3"/>
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    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
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        <property type="entry name" value="Importin-a_IBB"/>
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    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
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    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
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        <property type="entry name" value="IMPORTIN ALPHA"/>
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    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
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    </dbReference>
    <dbReference type="Pfam" id="PF01749">
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    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00185">
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        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51214">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
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            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
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        <location>
            <begin position="142"/>
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    </feature>
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        <location>
            <begin position="185"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="30"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
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    </feature>
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        <location>
            <begin position="17"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="48"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="85"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353622.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033240328.1"/>
        </source>
    </evidence>
    <sequence length="548" mass="60781" checksum="7D67975B7FFC23F5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCS6</accession>
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        <submittedName>
            <fullName evidence="10 11 12">Protein suppressor of sable isoform X1</fullName>
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        <name evidence="10 11 12" type="primary">su(sable)</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2025-04" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11 12">MV-25-SWS-2005</strain>
            <tissue evidence="10 11 12">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355566.3">
        <property type="nucleotide sequence ID" value="XM_001355530.4"/>
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    <dbReference type="RefSeq" id="XP_033241220.1">
        <property type="nucleotide sequence ID" value="XM_033385329.1"/>
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    <dbReference type="RefSeq" id="XP_033241221.1">
        <property type="nucleotide sequence ID" value="XM_033385330.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008270">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13119">
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    </dbReference>
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    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
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    </dbReference>
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    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="329"/>
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        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="357"/>
            <end position="380"/>
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    </feature>
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        <location>
            <begin position="329"/>
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        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="357"/>
            <end position="380"/>
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        <location>
            <begin position="1"/>
            <end position="48"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="76"/>
            <end position="325"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="523"/>
            <end position="584"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="643"/>
            <end position="668"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="710"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="830"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1013"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1093"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1395"/>
            <end position="1439"/>
        </location>
    </feature>
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        <location>
            <begin position="449"/>
            <end position="505"/>
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    </feature>
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        <location>
            <begin position="10"/>
            <end position="29"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="30"/>
            <end position="43"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="99"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="133"/>
            <end position="144"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="173"/>
            <end position="187"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="188"/>
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        <location>
            <begin position="218"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
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            <begin position="287"/>
            <end position="298"/>
        </location>
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            <begin position="544"/>
            <end position="560"/>
        </location>
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            <begin position="643"/>
            <end position="652"/>
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        <location>
            <begin position="659"/>
            <end position="668"/>
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            <begin position="710"/>
            <end position="722"/>
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            <begin position="757"/>
            <end position="769"/>
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            <begin position="1408"/>
            <end position="1423"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
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    </evidence>
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        <source>
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            <dbReference type="PROSITE" id="PS50103"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355566.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033241220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033241221.1"/>
        </source>
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    <sequence length="1439" mass="155812" checksum="38C0350A5F3905E0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDJ1</accession>
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        <submittedName>
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        <name evidence="13" type="primary">sbr</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <text evidence="2">Belongs to the NXF family.</text>
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        <property type="project" value="InterPro"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50177"/>
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            <dbReference type="PROSITE" id="PS51281"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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            <dbReference type="RefSeq" id="XP_033240634.1"/>
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    <sequence length="687" mass="77182" checksum="5121A4F1B2B20D71" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKG1</accession>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901844</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Hexapoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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        </lineage>
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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    </dbReference>
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        <location>
            <begin position="1"/>
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        </location>
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    <feature type="chain" id="PRO_5014300069" evidence="2">
        <location>
            <begin position="16"/>
            <end position="208"/>
        </location>
    </feature>
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            <begin position="183"/>
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        </location>
    </feature>
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        <location>
            <begin position="193"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015040882.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="23198" checksum="84FF8A208FD7FDD2" modified="2008-10-14" version="1">MIRALLALWSPSAVSVSTEDEAQNQNLAQAQSARQIRARWGMRRQSQSGNVYPRFRHFSRHARMIAFEMAGRQNLANLLDRVEPNFHQPQAEQDPDLSESEPDEGFLLQRYGGGYAVIRRRCNFTLPRIGIDEPVSQPLVWLALETLEAEAFNVNDIQGTRSSADFVAQFLVKNLTIDVFNAGNQPGYGPSSVRPPPPPPPPPPPAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKR7</accession>
    <accession>A0A6I8UYJ0</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC6901955</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133489.1">
        <property type="nucleotide sequence ID" value="XM_002133453.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKR7"/>
    <dbReference type="GeneID" id="6901955"/>
    <dbReference type="KEGG" id="dpo:6901955"/>
    <dbReference type="HOGENOM" id="CLU_2348879_0_0_1"/>
    <dbReference type="InParanoid" id="B5DKR7"/>
    <dbReference type="OMA" id="NELQPDY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244189">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002133489.1"/>
        </source>
    </evidence>
    <sequence length="97" mass="11029" checksum="1671F3422A439E28" modified="2008-10-14" version="1">MSDQNKLQPDNDPEYEELLKYFKSNNLISDSPSVTVRPGRVKITADGVVIVDQGTNMRRRNLKAVLTLPDLKERASYSSHKWLYELSLKLAEDPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>B5DP77</accession>
    <accession>A0A6I8V0A7</accession>
    <name>B5DP77_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Organic cation/carnitine transporter 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6900918</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134701.2">
        <property type="nucleotide sequence ID" value="XM_002134665.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DP77"/>
    <dbReference type="STRING" id="46245.B5DP77"/>
    <dbReference type="GeneID" id="6900918"/>
    <dbReference type="KEGG" id="dpo:6900918"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_15_1"/>
    <dbReference type="InParanoid" id="B5DP77"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250663">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DP77">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF37">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="45"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002134701.2"/>
        </source>
    </evidence>
    <sequence length="500" mass="56006" checksum="897904B7A368CA70" modified="2016-01-20" version="2">MLRCRLVSAGKLKTEVQNKQKRGGPIMELDEALKAIGFGCGQVLVLVFSSLANLLAVNETFGMSIVVVASSCDLETTAGQKSLMMTAMMAGMTISGFYVGYLIDIKGRVRILRWMLLVTILLSVISALMPEVYSLSAMRFLVGVFLCGPATCILGYMTEFSAPHARPPLVVLFSLGVGISLIYCPLVAKFFLPERICANEDGSYVVRSWRFLMMLYTLPGLFGLLGLWFLPESPYYLMSVKRKEETYEVLDWLCRRNRKQIEDLNIDLMDFPRDTIVAKANYFKLVWNDTIRLVKPPYCRDFMVCTLLSFGMFLISLGLGMWYPLLRNQDNSQHRLLCDVLSQPKNFDQPKSNVSATNEMPNFTDPLYYGIAYILFYFLALFLLVFISRKHVFVVYVSLAAACGLALNFIKEPIVILICFSAMMILPGVLIILVSSVLVDCMPTQLRAKALSLNKALSRLGAIIGCLLVGLLLKVSCLATLNIFVAYLIFCIILCFLLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D63</accession>
    <accession>A0A6I8UBW0</accession>
    <name>Q29D63_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pav</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353050.2">
        <property type="nucleotide sequence ID" value="XM_001353014.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D63"/>
    <dbReference type="SMR" id="Q29D63"/>
    <dbReference type="FunCoup" id="Q29D63">
        <property type="interactions" value="569"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29D63"/>
    <dbReference type="GeneID" id="4812666"/>
    <dbReference type="KEGG" id="dpo:4812666"/>
    <dbReference type="eggNOG" id="KOG0247">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_13_0_1"/>
    <dbReference type="InParanoid" id="Q29D63"/>
    <dbReference type="OMA" id="INPRIED"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071750">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01368">
        <property type="entry name" value="KISc_KIF23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4330:FF:000002">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4330">
        <property type="entry name" value="Kinesin-like protein Kif23, Arf6-interacting domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032384">
        <property type="entry name" value="Kif23_Arf-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038105">
        <property type="entry name" value="Kif23_Arf-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF600">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16540">
        <property type="entry name" value="MKLP1_Arf_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="685"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="609"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="124"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353050.2"/>
        </source>
    </evidence>
    <sequence length="887" mass="101032" checksum="672515B9B872F59F" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E83</accession>
    <accession>A0A6I8VLE2</accession>
    <name>Q29E83_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6 7" type="primary">LOC4811940</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
            <tissue evidence="4 5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352680.1">
        <property type="nucleotide sequence ID" value="XM_001352644.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043762.1">
        <property type="nucleotide sequence ID" value="XM_015188276.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238267.1">
        <property type="nucleotide sequence ID" value="XM_033382376.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238268.1">
        <property type="nucleotide sequence ID" value="XM_033382377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E83"/>
    <dbReference type="GeneID" id="4811940"/>
    <dbReference type="KEGG" id="dpo:4811940"/>
    <dbReference type="eggNOG" id="ENOG502RSTS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_947360_0_0_1"/>
    <dbReference type="OMA" id="EPVYVWI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073318">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29E83">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41155">
        <property type="entry name" value="FI19525P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41155:SF1">
        <property type="entry name" value="FI19525P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015043762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033238267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033238268.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="36110" checksum="1CEECF6224D5A345" modified="2006-04-04" version="1">MHERQVKKLKGSHYVATHGRLTPDPPYVHSNRGYSTDGEESHSHRAPSERTYSEYTLTHERISPQSQYNSSRKKRSANGHHQHLQHSYSHSQMGLSSSPDNGGPRSLSGPPPTRQPPRAPSALSYDHGGDGGSDIYVTSAAYKAPSEISRYSQRPVSRGPPRSVYSVASTAKTGRSARSTTKRGAKIETMSAPNPFCPNVKGVCCLMLLLNLGLILVTLGFVIVVQFYEPLYVWILGIVFLIFGFLTLIGCMIYCVYVCRDARTPSQVRNEDLYWTRHWQKNIGYTPQEINYKADKYDAYSDRYSVSKLSGKYSDRESQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EV6</accession>
    <accession>A0A6I8UAL8</accession>
    <name>Q29EV6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ras-related protein Rac1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Rac1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352457.1">
        <property type="nucleotide sequence ID" value="XM_001352421.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EV6"/>
    <dbReference type="SMR" id="Q29EV6"/>
    <dbReference type="FunCoup" id="Q29EV6">
        <property type="interactions" value="924"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EV6"/>
    <dbReference type="GeneID" id="4812255"/>
    <dbReference type="KEGG" id="dpo:4812255"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_3_1"/>
    <dbReference type="InParanoid" id="Q29EV6"/>
    <dbReference type="OMA" id="RRMAPIT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075341">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EV6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046843">
        <property type="term" value="P:dorsal appendage formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007394">
        <property type="term" value="P:dorsal closure, elongation of leading edge cells"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007390">
        <property type="term" value="P:germ-band shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008258">
        <property type="term" value="P:head involution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035099">
        <property type="term" value="P:hemocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035011">
        <property type="term" value="P:melanotic encapsulation of foreign target"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008078">
        <property type="term" value="P:mesodermal cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006911">
        <property type="term" value="P:phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090303">
        <property type="term" value="P:positive regulation of wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903391">
        <property type="term" value="P:regulation of adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050770">
        <property type="term" value="P:regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007426">
        <property type="term" value="P:tracheal outgrowth, open tracheal system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01871">
        <property type="entry name" value="Rac1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000088">
        <property type="entry name" value="Ras-related C3 botulinum toxin substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352457.1"/>
        </source>
    </evidence>
    <sequence length="192" mass="21354" checksum="F910B54BDBD8AFA3" modified="2006-04-04" version="1">MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDAKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWYPEVRHHCPSTPIILVGTKLDLRDDKNTIEKLRDKKLAPITYPQGLAMAKEIGAVKYLECSALTQKGLKTVFDEAIRSVLCPVLQPKSKRKCALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q29F07</accession>
    <accession>A0A6I8UAI6</accession>
    <name>Q29F07_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ragulator complex protein LAMTOR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Lamtor1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LAMTOR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352406.3">
        <property type="nucleotide sequence ID" value="XM_001352370.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29F07"/>
    <dbReference type="FunCoup" id="Q29F07">
        <property type="interactions" value="1264"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29F07"/>
    <dbReference type="GeneID" id="4812127"/>
    <dbReference type="KEGG" id="dpo:4812127"/>
    <dbReference type="eggNOG" id="ENOG502RYX2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2326611_0_0_1"/>
    <dbReference type="InParanoid" id="Q29F07"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072857">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001919">
        <property type="term" value="P:regulation of receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028209">
        <property type="entry name" value="LAMTOR1/MEH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13401">
        <property type="entry name" value="RAGULATOR COMPLEX PROTEIN LAMTOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13401:SF2">
        <property type="entry name" value="RAGULATOR COMPLEX PROTEIN LAMTOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15454">
        <property type="entry name" value="LAMTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01262">
        <property type="entry name" value="LAMTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001352406.3"/>
        </source>
    </evidence>
    <sequence length="180" mass="20596" checksum="A8F4F03DBDB374E9" modified="2016-01-20" version="3">MEYVHSVWNYIASVCSCLHCAEDVGAQNIEPNERTHLLVDPVNHSPALRRSNSDGLSNDYSHSLPKKDDQTALSRLVQNTAINMINVGAMDCHSLEHQEYTDRIKTYSQRLHELWNNVQHPNITRKGLLKDVPSHQFYITKPIYAKDTLQMKLFIEKALIGVNQIQIDHKEAVVVPFQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q29G66</accession>
    <accession>A0A6I8UGM4</accession>
    <name>Q29G66_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein 178B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4814612</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355187.1">
        <property type="nucleotide sequence ID" value="XM_001355151.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29G66"/>
    <dbReference type="FunCoup" id="Q29G66">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="GeneID" id="4814612"/>
    <dbReference type="KEGG" id="dpo:4814612"/>
    <dbReference type="eggNOG" id="ENOG502QT4K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061034_0_0_1"/>
    <dbReference type="InParanoid" id="Q29G66"/>
    <dbReference type="OMA" id="DSYCFNI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079559">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29G66">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000049">
        <property type="entry name" value="GH17940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21215">
        <property type="entry name" value="LD36024P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21215:SF0">
        <property type="entry name" value="LD36024P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001355187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="32909" checksum="AF4E88E162760A3A" modified="2006-04-04" version="1">MPCSAVTLSIATICAIIATALLAIAFSTDNWLHYDVWRNQIQAFASKHSDAESLLYNMNMKYYYYTRTRGLFRICYPKERPPASAVPTYLSPIETHCSNIDYFPQAEDEKIANEDATSRLHLARSCIALFIISFVTIFCAFWTGLSGCWKRSSGAITATSILLLVTCLLAAGAMGLWHTVEFFEKEKVVGEDYFQQWNTVLRDNTKIAYDWSYIVAWAGIGTCLLSAILLSGAAVCLRNEREKEEQLNLQYLMPVYSQKQPPYPPYANYAQPQIYPGPYYHGSQYGPYNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HA3</accession>
    <accession>A0A6I8UFS8</accession>
    <name>Q29HA3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane protein 41 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">stas</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM41 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354800.2">
        <property type="nucleotide sequence ID" value="XM_001354764.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HA3"/>
    <dbReference type="SMR" id="Q29HA3"/>
    <dbReference type="FunCoup" id="Q29HA3">
        <property type="interactions" value="1441"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29HA3"/>
    <dbReference type="GeneID" id="4815135"/>
    <dbReference type="KEGG" id="dpo:4815135"/>
    <dbReference type="eggNOG" id="KOG3140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038944_0_1_1"/>
    <dbReference type="InParanoid" id="Q29HA3"/>
    <dbReference type="OMA" id="CIKIPRD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081043">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HA3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045014">
        <property type="entry name" value="TM41A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43220:SF18">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 41B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="8">
        <location>
            <begin position="179"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001354800.2"/>
        </source>
    </evidence>
    <sequence length="341" mass="38158" checksum="0D3F16C0F93ADD9F" modified="2008-10-14" version="2">MACRMENNSRASGSGNGDALPTSSLNGAITDKMATMNLDRSKTTTTTMRSNGKHKPPMEELAQLLQQQEQQSPQELQQQPQQQQQTPQKQAMSADERKATKKSLVIVAAIFVASVATMCYVYAIFPELNESEKKHLKIPRDIQDAKMLAKVLDRYKDMYYFEVMFGVVVAYVFLQTFAIPGSLFLSILLGFLYKFPIALFLICFCSALGATLCYTLSNLVGRRLIRHFWPKKTSEWSKHVEEYRDSLFNYMLFLRMTPILPNWFINLASPVIGVPLHTFALGTFCGVAPPSVIAIQAGKTLQKMTSSSEAFSWTSMGILTVCACASLLPGLLKNKFKKKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IG1</accession>
    <accession>A0A6I8UHK5</accession>
    <name>Q29IG1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SET domain-containing protein SmydA-8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SmydA-9</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355633.1">
        <property type="nucleotide sequence ID" value="XM_001355597.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IG1"/>
    <dbReference type="FunCoup" id="Q29IG1">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IG1"/>
    <dbReference type="GeneID" id="4815979"/>
    <dbReference type="KEGG" id="dpo:4815979"/>
    <dbReference type="eggNOG" id="KOG2084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024539_2_0_1"/>
    <dbReference type="InParanoid" id="Q29IG1"/>
    <dbReference type="OMA" id="MKKGFTC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071467">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IG1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053010">
        <property type="entry name" value="SET_SmydA-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 4, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46455:SF3">
        <property type="entry name" value="SET AND MYND DOMAIN CONTAINING, ARTHROPOD-SPECIFIC, MEMBER 9, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355633.1"/>
        </source>
    </evidence>
    <sequence length="496" mass="55405" checksum="704E8A82200F70DC" modified="2006-04-04" version="1">MICPKRTSELLDLHLAPFKDKDPAWEVGVSKIAGRGLMATRSIKRGEIIFKDMPLLISLSAHEEDSLNACSVCLRELPDTRFMCRIGCGLPVCSLCSKKKQHKTDCDLLKSWGPNEPEVANSVIIRLLCVARALNLNKDQRDLIYCLQANLDNNHRVEVRNAAKCFKNFPTDKKIFEIMNRTVAVLRTNGFDKTTDRTNDNQEFNYRALYPLFAVMNHDCIPNSYYTFEEKTNIMIVRAAVDIPEGFEITTTYTKLFTGNIARHLFLKIKRGFTCKCSRCSDPTEKGAYISGLYCRDTNCSGLVVPETTGLPHPSYNCLVCKQKSTHAQMMKSQDFASGAINAKANSNSLRTLVQYLNEKSDNFIPNSNYVVIDAKLSVLQRLGEGREDCSEELAANTRLRYSRDVIQVMDQLGLGESLVRTQLQDLLRREEERRAKRLAEEAEKQRKLAELQAKAAEADKLLAALEEAARAEEAAAAPPVAAPAPAPAPAATETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JU0</accession>
    <accession>A0A6I8UI20</accession>
    <name>Q29JU0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Catalase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CatB</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the catalase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355812.1">
        <property type="nucleotide sequence ID" value="XM_001355776.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JU0"/>
    <dbReference type="FunCoup" id="Q29JU0">
        <property type="interactions" value="843"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JU0"/>
    <dbReference type="GeneID" id="4816148"/>
    <dbReference type="KEGG" id="dpo:4816148"/>
    <dbReference type="eggNOG" id="KOG0047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010645_2_0_1"/>
    <dbReference type="InParanoid" id="Q29JU0"/>
    <dbReference type="OMA" id="QVTWLFG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081678">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JU0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000001">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="9">
        <location>
            <begin position="26"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001355812.1"/>
        </source>
    </evidence>
    <sequence length="506" mass="57987" checksum="7CAE51890BCDEE56" modified="2006-04-04" version="1">MCSRDPASNQLIDYKTNDSEVQKDCTTASGTPVGVKDAIMTVGPRGPALLQDFVYLDEVMHFDTERIPERVAYAKGAGAFGYFECTHDITRFCAGSLFEKVRKRTAIAMRFSVACGENGSADTIREQRGFAVKFYTDDGIWDIVGCNMPVYYVRDPSLFPSLIHAQKRNPQTHLRDADMFWDFMTLRPETLHALLMYFSDRGTPDGYRHMHGYGAHTYRMVNPNGETFYVKFHFKTDQGIKNMDARRCSELMAHDPDYAIRDLYNSIKKGNYPSWTMYIQVMLNEEAKKSRFNPFDVTKVWPQKEYPLLPVGKLVLDRNPTNYFTEVEQLAFSPAHMIPGIEPSPDKMLQGRLFAYGDTQRHRLGVNYMQIPVNCPYRVHVKNFQRDGSMTVTDNQDGAPNYFPNSFCGPKESSRALALQTCCPISGDVYRFMSGDTEDNFSQVTDFWTYTLDNCGRKRLIRNLSEHLCEASQFLQERAVKLFTMVHSDFGRLMTEALNTARISKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LG7</accession>
    <accession>A0A6I8UKQ9</accession>
    <name>Q29LG7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrator complex subunit 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IntS14</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Integrator subunit 14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357012.1">
        <property type="nucleotide sequence ID" value="XM_001356976.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LG7"/>
    <dbReference type="SMR" id="Q29LG7"/>
    <dbReference type="FunCoup" id="Q29LG7">
        <property type="interactions" value="2708"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LG7"/>
    <dbReference type="GeneID" id="4817610"/>
    <dbReference type="KEGG" id="dpo:4817610"/>
    <dbReference type="eggNOG" id="ENOG502QQ37">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041485_0_0_1"/>
    <dbReference type="InParanoid" id="Q29LG7"/>
    <dbReference type="OMA" id="QSSVVWI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078432">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039841">
        <property type="entry name" value="INTS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045814">
        <property type="entry name" value="IntS14_b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046471">
        <property type="entry name" value="IntS14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13532">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13532:SF3">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19435">
        <property type="entry name" value="IntS14_b-barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20504">
        <property type="entry name" value="IntS14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="5">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 14 beta-barrel" evidence="6">
        <location>
            <begin position="223"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 14 beta-barrel" evidence="6">
        <location>
            <begin position="358"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 14 C-terminal" evidence="7">
        <location>
            <begin position="462"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001357012.1"/>
        </source>
    </evidence>
    <sequence length="582" mass="64630" checksum="454DD5976D430ADF" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MQ3</accession>
    <accession>A0A6I8UJX1</accession>
    <name>Q29MQ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Etoposide-induced protein 2.4 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tank</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EI24 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356576.2">
        <property type="nucleotide sequence ID" value="XM_001356540.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MQ3"/>
    <dbReference type="FunCoup" id="Q29MQ3">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MQ3"/>
    <dbReference type="GeneID" id="4817477"/>
    <dbReference type="KEGG" id="dpo:4817477"/>
    <dbReference type="eggNOG" id="KOG3966">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031164_0_1_1"/>
    <dbReference type="InParanoid" id="Q29MQ3"/>
    <dbReference type="OMA" id="KCCLLNG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073888">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MQ3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059112">
        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21389:SF0">
        <property type="entry name" value="ETOPOSIDE-INDUCED PROTEIN 2.4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21389">
        <property type="entry name" value="P53 INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
        <property type="entry name" value="EI24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="386"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="436"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="469"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356576.2"/>
        </source>
    </evidence>
    <sequence length="497" mass="56902" checksum="FEBEA0AD1AB61C17" modified="2008-10-14" version="2">MAAIKNIAYGILYGLWDSIRGMTLVLHIDNEVNRQDAEQDIRLLRRTDKDRYERARRSPSPVPSSAAAMIREEYAKRAEENSDERSLQKILNQKPTDEKEKQPQGEKKIAKKLFKCCMLNGGFTWMSIVLFESALLPTLKFCLTIFYGAQSETLPVVWAWLHPILSLLFGMMWVLPIFMLSKIVSSLWFADIANAAYRVRKGRRQLIPGISKLVADFLFSMVVQMLFLVQSMLVNLVPVKYVGSSLCFVHLCLLYSLYSFEYKWFNMGWELHRRLTYIEKNWPYFFGFGIPLTVLTNLSSSVIVSSCIFSIFFPLFILSGNEAQPMVDTTEISLRLFSPVVFISNLCFGGNPWSKANRLSAMQRQQYELQQRQRLLQREEQLLKQRKQQYMEQQRMQMRRERSHSRSQTPQSAPGTGPPYRYAQAPVFDAARVRDSSASSTHSSTSPSISSYRPPPYYGSSLRGATPLVPTPVPGAPQAPLTPPVIRQESGPDDWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29N71</accession>
    <accession>A0A6I8UJ84</accession>
    <name>Q29N71_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sorting nexin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Snx1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356408.1">
        <property type="nucleotide sequence ID" value="XM_001356372.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29N71"/>
    <dbReference type="FunCoup" id="Q29N71">
        <property type="interactions" value="2488"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29N71"/>
    <dbReference type="GeneID" id="4817212"/>
    <dbReference type="KEGG" id="dpo:4817212"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022783_0_0_1"/>
    <dbReference type="InParanoid" id="Q29N71"/>
    <dbReference type="OMA" id="LWETFLM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075476">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07623">
        <property type="entry name" value="BAR_SNX1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06859">
        <property type="entry name" value="PX_SNX1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000047">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000012">
        <property type="entry name" value="Sorting nexin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001356408.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="51991" checksum="32FDBF1506139C5E" modified="2006-04-04" version="1">MEVESPEHNKEFAEVDINNGAESASDDEEEVPAPGSVTLENENDLFVSAMSPSSIGDVHPLQEVLTDDGDFFIKIVVSDPQKIGDGMGSYLAYKVTTKTNIPKFKRTDFSTLRRFSDFLGIHDLLVGKYMRVGRIIPPAPSKNIIGSTKVKISPQQSEPGTPMNQEWVEIRRAALERFVHRTAQHPILRVDLDFMNFLESDQDLPRAVNTSALSGAGVIRLFNKVGETVNKITYKMDENDPWFDDKITEVENLDANLQKLHNAMKSLVTSRRELSALTGLVAKSAAMLSTCEEHTGLSRALSNLADVEEKMELLRSEQANSDFFILAEFIKDYLGLFGAIKCIFHERVKSFQNWQYAQMQLSKRRENRGRFELANRADKLDQAQLEVDEWQGKVQRCQQHFDDISAEIKREMERFELTRVKDFKANIIKYIEDQMAHQQQIVSYWEAFAPFAREIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NM0</accession>
    <accession>A0A6I8UKV0</accession>
    <name>Q29NM0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">UbiA prenyltransferase domain-containing protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">heix</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357132.2">
        <property type="nucleotide sequence ID" value="XM_001357096.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NM0"/>
    <dbReference type="FunCoup" id="Q29NM0">
        <property type="interactions" value="1444"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NM0"/>
    <dbReference type="GeneID" id="4817716"/>
    <dbReference type="KEGG" id="dpo:4817716"/>
    <dbReference type="eggNOG" id="KOG4581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043611_0_0_1"/>
    <dbReference type="InParanoid" id="Q29NM0"/>
    <dbReference type="OMA" id="QWIEGAR"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079194">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042371">
        <property type="term" value="P:vitamin K biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000005">
        <property type="entry name" value="UbiA prenyltransferase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00751">
        <property type="entry name" value="menA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929:SF0">
        <property type="entry name" value="UBIA PRENYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005355">
        <property type="entry name" value="UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001357132.2"/>
        </source>
    </evidence>
    <sequence length="354" mass="38885" checksum="0BD6A1300CB83C58" modified="2008-10-14" version="2">MATSRNESLVPDATQLLQERRHQPNGTASATATNGKASTAPTTSTMMANSGTFMKFKTYLLALRPWSLSASLVPTLLGSALAYRSQWAAEFSLLTFFLTAFTAVTVHCAGNVVNTYFDFIKGIDKQKADDRTLVDHILTKDEVVSLGAILYMAGCGGFVLLAVLSPAKMEHLALIYFGGLSSSFLYTGGIGFKYIALGDLVILILFGPISVLFAFMSQTGHVDWTTMGYAIPLALNTEAILHSNNTRDAESDCKAGIVTLAILIGRTASHVLYAMLLFTPYSLFFIFGLKYSLWFLLPLVTLPQAFKIEKRFRNEQTMHLVPRQTAKLNFFFGILYIVACCCAQQLPTFVLRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LYQ7</accession>
    <accession>A0A6I8UE85</accession>
    <name>Q2LYQ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized oxidoreductase YjmC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812923</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH2/MDH2 oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354066.2">
        <property type="nucleotide sequence ID" value="XM_001354030.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LYQ7"/>
    <dbReference type="SMR" id="Q2LYQ7"/>
    <dbReference type="STRING" id="46245.Q2LYQ7"/>
    <dbReference type="GeneID" id="4812923"/>
    <dbReference type="KEGG" id="dpo:4812923"/>
    <dbReference type="eggNOG" id="ENOG502S0M6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040452_3_0_1"/>
    <dbReference type="InParanoid" id="Q2LYQ7"/>
    <dbReference type="OMA" id="TNTEPAM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070420">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LYQ7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.60">
        <property type="entry name" value="Hypothetical oxidoreductase yiak, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043144">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043143">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091:SF0">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02615">
        <property type="entry name" value="Ldh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="L-sulfolactate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354066.2"/>
        </source>
    </evidence>
    <sequence length="440" mass="46341" checksum="01121D7929D86948" modified="2008-10-14" version="2">MTSLTLFCFLRRSAVTGDAAYAYAYASAANRRCVQTLQRRPSCRRHATTAAAVAAAAKAPNSNANSGDFAPLLRGSTRNMASSAPKLVAVAESRRFMIDCFKAVKVPQAHAEAQADLLVAADHRGHFSHGMNRLEMYINDLAINSTDGGAEPKVLKETPATAWVDGLNGLGAVVGNYCMDLAIKKAKTVGVGWVCAKGSNHYGMAGWYAIRAMEQGLVGMSMTNTSPLMAPTRAKEAALGTNPLSLGAPAANGDKFLLDMATTAVAVGKIEIQRRKGAALPDGWAQDPNGNITNDAELGFSTGCLMPLGGSELTSGYKGFGLGAMVDILSGVMSGAHYSTQVRKWTHAGADSAADLGQVFIAVDPNCFAPNFEERMTDFNARLRGVEPTDPSKPVLLAGDKESQNMASVDAAGGIQYLENQLKTCASLAEKLNIKPLAFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M076</accession>
    <accession>A0A6I8UD52</accession>
    <name>Q2M076_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SOX domain-containing protein dichaete</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">D</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353547.1">
        <property type="nucleotide sequence ID" value="XM_001353511.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M076"/>
    <dbReference type="SMR" id="Q2M076"/>
    <dbReference type="FunCoup" id="Q2M076">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M076"/>
    <dbReference type="GeneID" id="4813823"/>
    <dbReference type="KEGG" id="dpo:4813823"/>
    <dbReference type="eggNOG" id="KOG0527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021123_1_0_1"/>
    <dbReference type="InParanoid" id="Q2M076"/>
    <dbReference type="OMA" id="HQSSGMH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079206">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF324">
        <property type="entry name" value="SOX DOMAIN-CONTAINING PROTEIN DICHAETE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="57"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001353547.1"/>
        </source>
    </evidence>
    <sequence length="395" mass="41465" checksum="7A06DF234C0FE04F" modified="2006-02-21" version="1">MATLSSHPNYGFHLGAGGGQGLELPSPSDYAHQGQLQLSPGMDMDIKRVLHYSQSLAAMGGSPNGNGLPSGPGMGHHHHMTPHHMHSAVSAQQTLSPNSSIGSAGSLGSQSSLGSSGMGNGSSIGSSGHANHQSSGMHSLATSPGQEGHIKRPMNAFMVWSRLQRRQIAKDNPKMHNSEISKRLGAEWKLLAESEKRPFIDEAKRLRALHMKEHPDYKYRPRRKPKNPMTAGPQGGLQMQANGMGQQKLGGGPGAGAGGYNPFHQLPPYFAPSHHLDQPYPVPYFGGFDPLALSKLHQSQAAAAAAANNQGHKVLESQQAPPQLPPTSLSSFYSGIYSGMSAPSLYAAHSANAAAAGLYTSSSTSSPGSSPGTITPNGMDGSMDSALRRPVPVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NXW6</accession>
    <accession>A0A6I8VHJ3</accession>
    <name>A0A0R3NXW6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microsomal glutathione S-transferase 1</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC6901928</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16438"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotrimer; The trimer binds only one molecule of glutathione.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAPEG family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="RefSeq" id="XP_015041006.1">
        <property type="nucleotide sequence ID" value="XM_015185520.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NXW6"/>
    <dbReference type="SMR" id="A0A0R3NXW6"/>
    <dbReference type="FunCoup" id="A0A0R3NXW6">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3NXW6"/>
    <dbReference type="GeneID" id="6901928"/>
    <dbReference type="InParanoid" id="A0A0R3NXW6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244204">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.550:FF:000002">
        <property type="entry name" value="Microsomal glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040162">
        <property type="entry name" value="MGST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689:SF6">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015041006.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="20145" checksum="6A78193B507D30DE" modified="2016-01-20" version="1">MSTNSSTMPMTPPKELFNLDNPVFCCYLFWATVLVVKMLLMSLLTAVQRFRFKLLGLVPLGMRRKIFPNQEDLFFKNTEVQFNDPHVERVRRAHRNDMENILPYFIMALIYISTNPDATVACNLFRVASLARIVHTLVYAVYPVPQPSRIIAFGCMLLITFYMAAVVALRTLSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P714</accession>
    <accession>A0A6I8VLW0</accession>
    <name>A0A0R3P714_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26533750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015043963.1">
        <property type="nucleotide sequence ID" value="XM_015188477.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P714"/>
    <dbReference type="GeneID" id="26533750"/>
    <dbReference type="KEGG" id="dpo:26533750"/>
    <dbReference type="InParanoid" id="A0A0R3P714"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0272737">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P714">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015043963.1"/>
        </source>
    </evidence>
    <sequence length="167" mass="18446" checksum="7D1F29C4D594841F" modified="2016-01-20" version="1">MKQMLPVLFLVLLAPSKIFGIDNCDSRCKLVEYQKALESAVAVHTAQNKVLSTVPAKLVLLTCRMADILGDWREPSIDFPQEGTNADRGLRYLPKIGEKELLSLSMGIRDTLMEADPSLQRPEFKELETLLSDIIPVPKEPEVDPDIFANAINSQCGQMCAGDGIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB53</accession>
    <name>A0A6I8UB53_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352657.3">
        <property type="nucleotide sequence ID" value="XM_001352621.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UB53"/>
    <dbReference type="KEGG" id="dpo:4812081"/>
    <dbReference type="InParanoid" id="A0A6I8UB53"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007970">
        <property type="entry name" value="DUF733"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05306">
        <property type="entry name" value="DUF733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352657.3"/>
        </source>
    </evidence>
    <sequence length="245" mass="27979" checksum="1D234B7EB614D3E8" modified="2020-08-12" version="1">MLEEGDKETLRENEGGFTRANRIQVIMQVARSKDMCQVNSSNCLADTAPEAEPEPAQVTATTAPDEEAEPEPEPEAEPVPYPRRRRAPIRNADPHRLNQFKQKLLEMESQQQQRNNRRGARPSVGYLLFQYQSELTRRHAEPTRLSRTKIHIIDGLVSHTIRHLKNCSVEELQACKRELVYKEQLRDQLKWSMKRRRMATTRSSSGSRGSSPAPMAKSPRTPPKPRPQGEDQTSKVSLFGPEIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UB66</accession>
    <name>A0A6I8UB66_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein FAM207A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4811973</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352730.3">
        <property type="nucleotide sequence ID" value="XM_001352694.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UB66"/>
    <dbReference type="FunCoup" id="A0A6I8UB66">
        <property type="interactions" value="842"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811973"/>
    <dbReference type="InParanoid" id="A0A6I8UB66"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352730.3"/>
        </source>
    </evidence>
    <sequence length="179" mass="20365" checksum="0401E8F017BB68FF" modified="2020-08-12" version="1">MAKTKKNIRAKAKGAVGVAKQKAQDVQAQLNKSERQERLLHKTLTPKKTTTKKEKSALKHKKLLQRFADSRKERKNVQSSKKKPVVGDLKPLRDALPSLSDLYKLVKSKSKDAAEQTALTEPEAPLSVKQKIKKKRADIVNRAKSFEKLVKDKHFKENPRGVIGSLVRNKYRTMEEDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBA6</accession>
    <name>A0A6I8UBA6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ras-related protein Rab-14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RabX6</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352799.3">
        <property type="nucleotide sequence ID" value="XM_001352763.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBA6"/>
    <dbReference type="SMR" id="A0A6I8UBA6"/>
    <dbReference type="FunCoup" id="A0A6I8UBA6">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812377"/>
    <dbReference type="InParanoid" id="A0A6I8UBA6"/>
    <dbReference type="OMA" id="FCEQCHT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071392">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBA6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001329">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352799.3"/>
        </source>
    </evidence>
    <sequence length="211" mass="23543" checksum="30B817D55B7950D8" modified="2020-08-12" version="1">MAIVRIPKQKVILCGDYGVGKSSLFRRFTNNTFVTETDRKSTLGLDHIDREYDVGDKQIKLQLWDTGGMERVASVTSSYYKFAEGAILVFALDNAASFHSLSQHLLDIVTYAENAKIFICGNKSDLEGREPEVSDEEVEAFCEQCHSLISATYKTSCRTGAGVEEMFRDISRQLVHANRSKMELQALEQKSFKIDTASDPTVNEEASSCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCH7</accession>
    <accession>Q2M0X2</accession>
    <name>A0A6I8UCH7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Probable chitinase 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4813398</name>
        <name evidence="11" type="synonym">obst-F</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353301.2">
        <property type="nucleotide sequence ID" value="XM_001353265.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238169.1">
        <property type="nucleotide sequence ID" value="XM_033382278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCH7"/>
    <dbReference type="SMR" id="A0A6I8UCH7"/>
    <dbReference type="GeneID" id="4813398"/>
    <dbReference type="KEGG" id="dpo:117184478"/>
    <dbReference type="KEGG" id="dpo:4813398"/>
    <dbReference type="OMA" id="GYCDLAK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634424" evidence="7">
        <location>
            <begin position="22"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="147"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="272"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353301.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238169.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="36165" checksum="7D7F4CF74F26F060" modified="2020-08-12" version="1">MQCFWAFVLGIICLRLGAGHAVDRGRQLQVQAAIGDLSHICDGRPDGELVPHPLDCSGYFVCARVPTLHYCDQGLQFDETRGACDLTENTNCRQSELLQASHRPANDFVADSSELIWWPQRPRPVFVAVDVASGLPVSPMAKYDPQHIECRHFGAYFLPHPSNCRLYFICAYGHLHRHQCGRGTFWNYEQSECQLSNSAVCYGQSRPDRQDADGGIDETSGTTTSSGGEVTVCYIVGTSGTSTVFSEPSTTDATVPLLEPVPSPPRAEASSPLTCPSEKQSYMSHPEDCSKYYICIGGLPVLTSCPKGLYWDQMSGYCDLAKNVKCFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCJ6</accession>
    <name>A0A6I8UCJ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vitellogenin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4813915</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353324.4">
        <property type="nucleotide sequence ID" value="XM_001353288.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCJ6"/>
    <dbReference type="FunCoup" id="A0A6I8UCJ6">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813915"/>
    <dbReference type="InParanoid" id="A0A6I8UCJ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000122">
        <property type="entry name" value="Vitellogenin-3-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF149">
        <property type="entry name" value="FI01450P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026274233" evidence="6">
        <location>
            <begin position="21"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="7">
        <location>
            <begin position="107"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353324.4"/>
        </source>
    </evidence>
    <sequence length="380" mass="42133" checksum="FF7911440E174F7B" modified="2020-08-12" version="1">MRIQCFLLWQISLHCHAIHAEMTAADGRSTPNWWEAVPRVLTDIVTVKANILTAAPLELAANAIDMLCSSTLFMDRVPPKITPDINKMHFQYMTPCHNYSVPLLDAAKLWNHSKFGKGRKVVILATGWTNTVNESSAIAMISRAFMCRRDVNFVIVDAADYVDTLYSWSALNTDLIGEHIAVGLTHLIELTPLRNIHLIGHSLGAHIMGTAGRTFKRLTGRLVPRITGLDPAKPCFRQEHILPGLTRGDAQLVDVIHTNIGILAKRGPLGDVDFYPGGAHPIQPGCLTIGCSHTRAVEYFAESAYPQQEKNFMGTKCSSWDELRRRDCRAGAGTPSSMGYIIDRKARGIYYVDVNGWPPYGRSSSKTANPRSKICHLCRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCY0</accession>
    <name>A0A6I8UCY0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353512.2">
        <property type="nucleotide sequence ID" value="XM_001353476.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCY0"/>
    <dbReference type="KEGG" id="dpo:4812752"/>
    <dbReference type="InParanoid" id="A0A6I8UCY0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCY0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026246651" evidence="2">
        <location>
            <begin position="22"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353512.2"/>
        </source>
    </evidence>
    <sequence length="163" mass="17247" checksum="EA19760B3746A820" modified="2020-08-12" version="1">MAKFLVLLLVIALSLLSLGDAQRYSQRLSRGRSRYSARQELAPAAAGDVDAVTPYPSADELKPEVPFDEAAAPAALDPTPDAVYGPPDAAPNSVPDEVYGPPDVTGNDLPAADIPAEQQARLVAARRAANYRRAGQPVAYRRAVSAAARPAKLSAARSVVRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEJ2</accession>
    <name>A0A6I8UEJ2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MED24</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 24 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354207.3">
        <property type="nucleotide sequence ID" value="XM_001354171.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEJ2"/>
    <dbReference type="FunCoup" id="A0A6I8UEJ2">
        <property type="interactions" value="946"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814045"/>
    <dbReference type="InParanoid" id="A0A6I8UEJ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UEJ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021429">
        <property type="entry name" value="Mediator_Med24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11277">
        <property type="entry name" value="Med24_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="847"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="869"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001354207.3"/>
        </source>
    </evidence>
    <sequence length="992" mass="111824" checksum="9F940C4848F6F3E5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFE4</accession>
    <name>A0A6I8UFE4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cathepsin B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CtsB</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354605.2">
        <property type="nucleotide sequence ID" value="XM_001354569.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFE4"/>
    <dbReference type="FunCoup" id="A0A6I8UFE4">
        <property type="interactions" value="988"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815015"/>
    <dbReference type="InParanoid" id="A0A6I8UFE4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFE4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02620">
        <property type="entry name" value="Peptidase_C1A_CathepsinB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000031">
        <property type="entry name" value="Cathepsin B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012599">
        <property type="entry name" value="Propeptide_C1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08127">
        <property type="entry name" value="Propeptide_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026299486" evidence="8">
        <location>
            <begin position="17"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="9">
        <location>
            <begin position="86"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001354605.2"/>
        </source>
    </evidence>
    <sequence length="338" mass="37095" checksum="295728F64C533D8E" modified="2020-08-12" version="1">MNLLLLAMTMAVAVSATEGPSMLSDEFIELVRSKASTWQVGRNFKESVSEEYIRGLMGVHPDAHKFALPEKRIVLGDLYADDGIDIPEEFDARKAWPNCPTIGEIRDQGSCGSCWAFGAVEAMSDRVCIHSEGKVNFHLSADDLVSCCHICGFGCNGGFPGAAWSYWTRKGIVSGGPYGSTQGCRPYEIAPCEHHVNGTRPPCSHGSTPSCQHKCQASYSVEYAKDKNFGSKSYSVRRNVAEIQQEIMTNGPVEGAFTVYEDLILYKSGVYQHEHGKELGGHAIRILGWGVWGESKVPYWLIGNSWNTDWGDNGFFRILRGQDHCGIESSISAGLPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG80</accession>
    <name>A0A6I8UG80_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 185B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4814541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355002.2">
        <property type="nucleotide sequence ID" value="XM_001354966.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UG80"/>
    <dbReference type="FunCoup" id="A0A6I8UG80">
        <property type="interactions" value="1907"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814541"/>
    <dbReference type="InParanoid" id="A0A6I8UG80"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019396">
        <property type="entry name" value="TM_Fragile-X-F-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568">
        <property type="entry name" value="FAM11A, B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10269">
        <property type="entry name" value="Tmemb_185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355002.2"/>
        </source>
    </evidence>
    <sequence length="358" mass="40741" checksum="BFD564B9E4B89659" modified="2020-08-12" version="1">MNLESLFRDFNPCKFIVHCSLFTFVTLYALRLDNYIDWPYWAIFTPLWIWKCTAILGAIVGAIVWCRYPHYRLEGDAYTQFKAMLISLALHLILLMFELLACDKLTSDRHLWVLVFIPLIFGSVVSVGACVWAVKHDRSFELELFLAVNALQFVSLPLKLDRFVYWNWDVVFVPMWIVICLSLVSVLYNIIFCGIMMRTPEVSLQQKKAALNAAVGNICTVLPLLCFQVVICDKLDGELKFPYIVVFSPLLVSILALIILSFTAKGGNMWWFGIRKSFSQFLLSAMPFLQEYGNISYHADTHSNAGQSVPLDAFSSTSTAAANEQLHEFSRHDKKNKKGAKNDILKPVVPFVSIDLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGS0</accession>
    <name>A0A6I8UGS0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Protein unc-93 homolog A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">LOC4815549</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355237.2">
        <property type="nucleotide sequence ID" value="XM_001355201.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240076.1">
        <property type="nucleotide sequence ID" value="XM_033384185.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240077.1">
        <property type="nucleotide sequence ID" value="XM_033384186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGS0"/>
    <dbReference type="KEGG" id="dpo:4815549"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGS0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055120">
        <property type="term" value="C:striated muscle dense body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043266">
        <property type="term" value="P:regulation of potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051951">
        <property type="entry name" value="UNC-93_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444">
        <property type="entry name" value="UNC-93 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444:SF11">
        <property type="entry name" value="UNC93-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="599"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="661"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="698"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="723"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001355237.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033240076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033240077.1"/>
        </source>
    </evidence>
    <sequence length="755" mass="82471" checksum="4F3E3BC85CD1142F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGT0</accession>
    <name>A0A6I8UGT0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">methylated diphthine methylhydrolase</fullName>
            <ecNumber evidence="6">3.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4815990</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42656"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="3.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH7 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.97" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001355259.3">
        <property type="nucleotide sequence ID" value="XM_001355223.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGT0"/>
    <dbReference type="FunCoup" id="A0A6I8UGT0">
        <property type="interactions" value="1525"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815990"/>
    <dbReference type="InParanoid" id="A0A6I8UGT0"/>
    <dbReference type="OMA" id="LDMKWLP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076531">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGT0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355259.3"/>
        </source>
    </evidence>
    <sequence length="359" mass="40405" checksum="6A47B216B3599289" modified="2020-08-12" version="1">MARFRTLHSVDTNYSADSVEWCAQDGHHAYFACGTYQLVEAEENEVTTKNRPRKGRVYLYHFDTGSGALERVQSIETAAVLDMKWLPAWSIDSGPLLATANALGEVEIYELLSDEKQLQRRMCLGLTAAEPTAEAPLALALDWQRDGDELQLVVSDSKGNINLLRYTPQGKLFVLSAWNAHSFEAWTCVFDRWTPHRVYSGGDDCLLQARDLRTEQLVWTNRAHQAGVTCLLSHPKHDYQLLTGSYDEQLRVFDTRMMKRSLAEVNLGGGIWRLKPHPNRAEIILAACMYNNFSVVQLDLENSAVTMLGTYDEHSSICYGADWNPSLSEGDGLMHMATCSFYDHKMCVSSFDLGCSQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHC2</accession>
    <name>A0A6I8UHC2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4815637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355408.3">
        <property type="nucleotide sequence ID" value="XM_001355372.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHC2"/>
    <dbReference type="KEGG" id="dpo:4815637"/>
    <dbReference type="InParanoid" id="A0A6I8UHC2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355408.3"/>
        </source>
    </evidence>
    <sequence length="502" mass="55158" checksum="DB03D13214E5B0E6" modified="2020-08-12" version="1">MSYQIKSTPFFRRMYNNSQRNGQLQRLREEYERIQEFNDRTIELKMRQVRLKRLFREIEEIKEGPPAMSGTGTSTGAVAGAGPGPGLDGTGTCNVGTSSATARSMQRRRRLELLAPTVEQQQYLQRLDTLARAKELGKEISRRNAELAEAGVGHYLRSDLRLTTKMQAAVNAKRAAADRRERAAKRISYGNAQSKHNLKRLVTSRLRRLCGGGSTGTGAAGAGLMDSSPGSSGQQGPVAGTNASRLQRPDLTKKAMLKQRLLYGNSRSCSNMRRLRRHNQIQGHGRDQAPVKLCSQVELLADFVEEGDDGDEEEQGLELPSESSGRLQMAHYTPSSSGTTDTITTEDLDDLEDFGELKDQGAPATILEGTSEEEDGSETEMCTVSRASTSQAIKQGSQCSRQVSMALQTEGTDRVHPEGAVSSTQDNTNNSHRISLTLPWFKAFPRLLDGTLPVPASTTESIASSSSYEDLATSNHSYGDDYSYPQHQGFVLPPFRFRKSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHK7</accession>
    <name>A0A6I8UHK7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein TwdlX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TwdlX</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355595.2">
        <property type="nucleotide sequence ID" value="XM_001355559.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHK7"/>
    <dbReference type="FunCoup" id="A0A6I8UHK7">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815553"/>
    <dbReference type="InParanoid" id="A0A6I8UHK7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026060942" evidence="1">
        <location>
            <begin position="19"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="2">
        <location>
            <begin position="140"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355595.2"/>
        </source>
    </evidence>
    <sequence length="343" mass="35671" checksum="3CC231B324D7D54B" modified="2020-08-12" version="1">MNQFVVLACLAVIGGSLAAPRPDVSHLGYRYQAGGYNGNLPSSVVSTPLVVGTSYQPTAAGSNYFSSAPSIGQLNAGSVSTGFVPSSSSSSGIGYQVGGAESSSVTVGDSIGLQGLQPGPTINYNEQESYISHLANFQPALVSKHFYIHSAPEDHDEQQIVRYVNVGRPQKNYRVVFINAPTSTTSKAKIIANVAPVEEKTAIYVLSKKSNALDVSAEVVTQRPVANKPEVFFVKYKTPQEAAHAQQTIQANYDALGGASETSNEGIIPVSSVIGSLGDNGASGVVTDASGSLNIVGTGGVPNADVNYNNVGFDAEGSQRQVISTINGSNAQATYLPARPVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHP4</accession>
    <name>A0A6I8UHP4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4815463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355659.3">
        <property type="nucleotide sequence ID" value="XM_001355623.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHP4"/>
    <dbReference type="FunCoup" id="A0A6I8UHP4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815463"/>
    <dbReference type="InParanoid" id="A0A6I8UHP4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHP4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355659.3"/>
        </source>
    </evidence>
    <sequence length="195" mass="22421" checksum="7713526F272FE1FA" modified="2020-08-12" version="1">MPLANERHSHYNSRLGHLLQKTASNLRSYQEFLSSQAQHLLAPVNRLWDRSQRYRLVAGHSTDERCATALLSECQDAYQSICHSIMQMNEMLDEMANDVADFDTECIYLSGELQPEPCPTSVAEWREWLNESLHSLQTQVKCLEIVSRLFLPLILEQRTVDEFKTYLQLDEHQEAVLCMGLAKAERQATLPLLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJP7</accession>
    <name>A0A6I8UJP7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="18">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">Btk</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">MV-25-SWS-2005</strain>
            <tissue evidence="25">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 26 18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="18"/>
    <dbReference type="RefSeq" id="XP_001356602.4">
        <property type="nucleotide sequence ID" value="XM_001356566.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJP7"/>
    <dbReference type="FunCoup" id="A0A6I8UJP7">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817015"/>
    <dbReference type="InParanoid" id="A0A6I8UJP7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007424">
        <property type="term" value="P:open tracheal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007435">
        <property type="term" value="P:salivary gland morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01238">
        <property type="entry name" value="PH_Btk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05059">
        <property type="entry name" value="PTKc_Tec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09934">
        <property type="entry name" value="SH2_Tec_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11768">
        <property type="entry name" value="SH3_Tec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000052">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000190">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001562">
        <property type="entry name" value="Znf_Btk_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00779">
        <property type="entry name" value="BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51113">
        <property type="entry name" value="ZF_BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 18 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 18" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="22">
        <location>
            <begin position="43"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="21">
        <location>
            <begin position="356"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="20">
        <location>
            <begin position="424"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="540"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="232"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="568"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_001356602.4"/>
        </source>
    </evidence>
    <sequence length="800" mass="89478" checksum="52BE129550F34C2F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK31</accession>
    <name>A0A6I8UK31_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MPN domain-containing protein CG4751</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4817300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M67 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356794.3">
        <property type="nucleotide sequence ID" value="XM_001356758.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UK31">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817300"/>
    <dbReference type="InParanoid" id="A0A6I8UK31"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK31">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08067">
        <property type="entry name" value="MPN_2A_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000053">
        <property type="entry name" value="MPN domain-containing protein CG4751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040843">
        <property type="entry name" value="RAMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18755">
        <property type="entry name" value="RAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="279"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="534"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="691"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="862"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="979"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1057"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1212"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1304"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1360"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1418"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="698"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="862"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="875"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="894"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1063"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1212"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1308"/>
            <end position="1323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356794.3"/>
        </source>
    </evidence>
    <sequence length="1441" mass="151891" checksum="3CE8467C567C49AE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKJ8</accession>
    <name>A0A6I8UKJ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein wry isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">wry</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356935.3">
        <property type="nucleotide sequence ID" value="XM_001356899.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UKJ8">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817520"/>
    <dbReference type="InParanoid" id="A0A6I8UKJ8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:signaling"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
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        <property type="entry name" value="Notch_Cell-Fate_Det"/>
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        <property type="match status" value="5"/>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
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            <begin position="18"/>
            <end position="1306"/>
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        <location>
            <begin position="1140"/>
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            <begin position="822"/>
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    <feature type="domain" description="EGF-like" evidence="16">
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            <begin position="862"/>
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            <begin position="1018"/>
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            <begin position="33"/>
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            <begin position="1196"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_001356935.3"/>
        </source>
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    <sequence length="1306" mass="140165" checksum="A399800ECD8E37E7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULV4</accession>
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        </submittedName>
    </protein>
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            <taxon>Arthropoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">MV-25-SWS-2005</strain>
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        </source>
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            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001357486.3"/>
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    </evidence>
    <sequence length="323" mass="37941" checksum="5723A124AB71FE4A" modified="2020-08-12" version="1">MDKAQTKQLLMYRTPNEAHNQIFIFNSPIDYKDQQLIVYQRPGVLCGENGFGYCVVYKRDHYILNNPPNFQSPTKRSFLNECIRQLYLMNGLVHKLYWGSSSASLATPRELWHQVDSCLQPERITKKIESLKRVKRQLFREPRCGRKTTKPVRGKVWGTQVFSFRPMREKIGRRHIRRGFLRPARQTVLQQMICENLKREMVCSPAVCQTVISAAEGIFNVNASVISDICSYHNNAARHQPAVECRLTSGQVRLRNNEACRLTQRAKRLEEMFIHEQVRRAVQNHQLILEESVRSIFYAKELFRLIEDHEEFVCTDKQILCRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMI5</accession>
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            <fullName evidence="3">Pre-mRNA-splicing factor 18</fullName>
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            <fullName evidence="8">PRP18 homolog</fullName>
        </alternativeName>
    </protein>
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        <name evidence="11" type="primary">Prp18</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP18 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001357740.5"/>
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        <property type="interactions" value="1602"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:4800512"/>
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    <dbReference type="OMA" id="SFAQVRW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079290">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:U2-type post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Pre-mRNA processing factor 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.110:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor 18 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039979">
        <property type="entry name" value="PRPF18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007:SF19">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02840">
        <property type="entry name" value="Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="9">
        <location>
            <begin position="74"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357777.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="39600" checksum="A8622B6ABA61853D" modified="2020-08-12" version="1">MDLLKKEIARKRKLLEDKQLIDDKKKYFKRGDLNAKNTEEVLQKVGYKKQESVEAQGQTTEGAYSFVADGQNILPRTEVIRRLRERGEPILIFGETEPEAFDRLRQCEISQPEANRGFRNDFQEAMEQVDAAYLQEMFANAPTAKEDKKSDLAELDESVSWESIQTMAQKMGRNKDYDMDVIITLLTFLLKLWNDQIATYSKHEIMSTKVKMTRVIYTQTKEYVKPLFRKLKHHTLPEDILDSLRDIVKHLLNRNYITASDAYLEMAIGNAPWPIGVTMVGIHARTGREKIFSKNVAHVMNDETQRKYIQGLKRLMTKCQEYFPTDPSKCVEYVSKKDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMV9</accession>
    <name>A0A6I8UMV9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polyribonucleotide nucleotidyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PNPase</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyribonucleotide nucleotidyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.8" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001357946.2">
        <property type="nucleotide sequence ID" value="XM_001357909.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMV9"/>
    <dbReference type="FunCoup" id="A0A6I8UMV9">
        <property type="interactions" value="1870"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800726"/>
    <dbReference type="InParanoid" id="A0A6I8UMV9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000958">
        <property type="term" value="P:mitochondrial mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09033">
        <property type="entry name" value="KH-I_PNPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11363">
        <property type="entry name" value="RNase_PH_PNPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11364">
        <property type="entry name" value="RNase_PH_PNPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000001">
        <property type="entry name" value="Polyribonucleotide nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000001">
        <property type="entry name" value="Polyribonucleotide nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000032">
        <property type="entry name" value="Polyribonucleotide nucleotidyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000113">
        <property type="entry name" value="polyribonucleotide nucleotidyltransferase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015848">
        <property type="entry name" value="PNPase_PH_RNA-bd_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036456">
        <property type="entry name" value="PNPase_PH_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03591">
        <property type="entry name" value="polynuc_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008805">
        <property type="entry name" value="PRK11824.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252:SF0">
        <property type="entry name" value="POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03726">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005499">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46915">
        <property type="entry name" value="Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="676"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357946.2"/>
        </source>
    </evidence>
    <sequence length="769" mass="84731" checksum="D1F18341AEA73149" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNE7</accession>
    <name>A0A6I8UNE7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Gr98a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358315.4">
        <property type="nucleotide sequence ID" value="XM_001358278.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNE7"/>
    <dbReference type="FunCoup" id="A0A6I8UNE7">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801182"/>
    <dbReference type="InParanoid" id="A0A6I8UNE7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358315.4"/>
        </source>
    </evidence>
    <sequence length="389" mass="45197" checksum="5A9367EF73ECF1B3" modified="2020-08-12" version="1">MRLMSGELDSCSLRRMHRVMKCLGIIPFGQHFYLKVLCNLFMVFVIGTASSWRFSFNYEFTYDFLNDHMSRILDLTNFLVLMSAHFTIVMEILWGNRSAEIEQQMEQILHDLRVHLGREVSLKRFRHYSNAIYGSLISRFLLLFGVAVYNNEGLVFSAMFSEAVMQLRFTEFSLYCGVALAFHQELCSAGSSLLVELHLTRFDLWPLRRFTLEKLSRLQQIHGRLWQTIRLIERNFQRSLSIMLLKFFVDTAALPYWLYLSKIQHTSVSVQYYCATEEFCKLMEIIVPCWLCSRCDLMQRKFRSIFYRLATGRRNGQLNAALIRICIQLGQEKYQFSAGGFAYISTEMLGTFLFGMISYIVIGIQFNLNFNASNSSKLAAEAAVTDAPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNR4</accession>
    <name>A0A6I8UNR4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine, alanine and asparagine-rich protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TwdlN</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358434.2">
        <property type="nucleotide sequence ID" value="XM_001358397.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNR4"/>
    <dbReference type="FunCoup" id="A0A6I8UNR4">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801319"/>
    <dbReference type="InParanoid" id="A0A6I8UNR4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:chitin-based cuticle development"/>
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        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026349404" evidence="1">
        <location>
            <begin position="16"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="2">
        <location>
            <begin position="143"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358434.2"/>
        </source>
    </evidence>
    <sequence length="309" mass="30926" checksum="44F0DD34AD557E0A" modified="2020-08-12" version="1">MRAFIVLCLVAVACADKLGYNYKPVGHSNSGLSFAPGSGSIGGGSIGGGSIGGGLQGLGGSLGGLGGLGGGSNFGSLDSGLGGLGLGSSDLGSSGFGSSGLGSSGLGSSGLGSSGLGSSGLGSSGLGGGFSGPVSYEAPAPSAELNKEFFTFSANEQDFDEPEALERVSSSVNKGLRVVFIKGPENRGLENAALALAKQAAQQETAIYVLNKQADIGDLASKLNAIRSNSNNKPEVHFVKYRTPEDAANAQRAIQSQYDQLGGSSQAHDGGVANALNFASAGPVRQAQAQIPENSYLPSSVLRRLRFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNS5</accession>
    <name>A0A6I8UNS5_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358449.2">
        <property type="nucleotide sequence ID" value="XM_001358412.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNS5"/>
    <dbReference type="GeneID" id="4801338"/>
    <dbReference type="KEGG" id="dpo:4801338"/>
    <dbReference type="InParanoid" id="A0A6I8UNS5"/>
    <dbReference type="OMA" id="CTSWVSA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026062878" evidence="2">
        <location>
            <begin position="23"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358449.2"/>
        </source>
    </evidence>
    <sequence length="70" mass="7479" checksum="8CE6B914CCC8ECA7" modified="2020-08-12" version="1">MKFLYMLLSICIFLLCAPWSTAQTIPTLALPDPTPPNGAPVEGDSTSFPGPPSASPTEPTPQVNTIYPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNU5</accession>
    <name>A0A6I8UNU5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear hormone receptor HR96</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Hr96</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358476.2">
        <property type="nucleotide sequence ID" value="XM_001358439.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNU5"/>
    <dbReference type="FunCoup" id="A0A6I8UNU5">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801369"/>
    <dbReference type="InParanoid" id="A0A6I8UNU5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06966">
        <property type="entry name" value="NR_DBD_CAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06929">
        <property type="entry name" value="NR_LBD_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000035">
        <property type="entry name" value="Nuclear hormone receptor HR96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000042">
        <property type="entry name" value="Nuclear hormone receptor HR96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF283">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="496"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="88"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="300"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001358476.2"/>
        </source>
    </evidence>
    <sequence length="736" mass="82868" checksum="E2E2CE26D7B26CC2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP67</accession>
    <name>A0A6I8UP67_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Serine protease gd</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4801618</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358680.2">
        <property type="nucleotide sequence ID" value="XM_001358643.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP67"/>
    <dbReference type="KEGG" id="dpo:4801618"/>
    <dbReference type="InParanoid" id="A0A6I8UP67"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000146">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031986">
        <property type="entry name" value="GD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF143">
        <property type="entry name" value="SERINE PROTEASE GD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16030">
        <property type="entry name" value="GD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026200520" evidence="9">
        <location>
            <begin position="23"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="206"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358680.2"/>
        </source>
    </evidence>
    <sequence length="449" mass="48476" checksum="21DA7520BB669022" modified="2020-08-12" version="1">MAIAPVLLLFVCLGLCLGTALAQTVPSHNCGDLFTYMRRGDGSIIGVFTPLQDGLTSLDWSVVFTAHGSSQAHTVSSMGGYPDKQQAFEKMRSGGRGQAFVSFQNYGNELPKLIKVELNGHVYCSASDYDAPSSTMTRGWKASVTGSRPSVPVTMPPHFTYSSRAPTSTGSNPFLNRARTGTQASVAPRTDPDFAECGVEGFAAAQAGGEVITRGRFPWLGALYEDNGPTLKYSCVVSLVSKRTVITAAHCIHGKKALDLWVYVGRHDRNTHPESGATLIRASSTRTPSQYDGNPVPDSDVGLVVLDTEVVYTNYVRPICLWTSRMGLPANEGDMGAVAGWGLDENAVETRYPKAVNVRVVTREQCLSDMKRAEDFITPNTLCAGNSNAHGPCLGDSGGGLMIMRNGRWTLRAIVALAPRKGLICDLRKYVIYCDVAKHLTWIESNIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPG6</accession>
    <name>A0A6I8UPG6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heat shock protein 68</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Hsp68</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358803.2">
        <property type="nucleotide sequence ID" value="XM_001358766.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPG6"/>
    <dbReference type="FunCoup" id="A0A6I8UPG6">
        <property type="interactions" value="553"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801762"/>
    <dbReference type="InParanoid" id="A0A6I8UPG6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000024">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="608"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="624"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001358803.2"/>
        </source>
    </evidence>
    <sequence length="642" mass="70267" checksum="22D07652C73D4F8D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPU8</accession>
    <name>A0A6I8UPU8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">ATP synthase F(0) complex subunit e, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC4801944</name>
        <name evidence="18 19" type="synonym">ATPsynE</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18 19">MV-25-SWS-2005</strain>
            <strain evidence="17 18">MV2-25</strain>
            <tissue evidence="18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 15">Subunit e, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP).</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATPase e subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358959.1">
        <property type="nucleotide sequence ID" value="XM_001358922.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037487.1">
        <property type="nucleotide sequence ID" value="XM_015182001.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPU8"/>
    <dbReference type="GeneID" id="4801944"/>
    <dbReference type="KEGG" id="dpo:4801944"/>
    <dbReference type="OMA" id="FCRWSLL"/>
    <dbReference type="Proteomes" id="UP000001819">
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        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial inner membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:proton-transporting ATP synthase complex"/>
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    </dbReference>
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        <property type="term" value="F:proton transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
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        <property type="entry name" value="ATP_synth_F0_esu_mt"/>
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        <property type="entry name" value="ATP SYNTHASE E CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP SYNTHASE SUBUNIT E, MITOCHONDRIAL"/>
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        <property type="entry name" value="ATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11 15" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 15" id="KW-0138">CF(0)</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="34"/>
            <end position="74"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007333"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00022547"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00057306"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00064647"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00074682"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367005"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_001358959.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015037487.1"/>
        </source>
    </evidence>
    <sequence length="81" mass="9067" checksum="2FA33E999257D290" modified="2020-08-12" version="1">MSQPPVRVSPLIKLGRWSLLLVGIAYGAAHQSRLSKKEEKVREIEAQQKVVRDAKLAEEKKRNAEAEARALAELSKPTPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ05</accession>
    <name>A0A6I8UQ05_DROPS</name>
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        <submittedName>
            <fullName evidence="6">26S proteasome non-ATPase regulatory subunit 12</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="primary">Rpn5</name>
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    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
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        <text evidence="1">Belongs to the proteasome subunit p55 family.</text>
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        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
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        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
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            <end position="459"/>
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            <end position="300"/>
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            <dbReference type="ARBA" id="ARBA00022942"/>
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        </source>
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            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359057.1"/>
        </source>
    </evidence>
    <sequence length="500" mass="57226" checksum="DADE32A34EF5EA47" modified="2020-08-12" version="1">MDTYLFDGGRITKMEVDYEPACNEKIPLAKELAKKNPDAFHEAIEMMLQLEKQTRLGADMVSCSRVLVAICQICFDASNWNALNEYVTLLVRRRSQLKQAVVKMIQECCTYVDKTPNKETKLKLIDTLRSVTEGKIYVEIERARLTKTLADIKEADGDVAAAASVMEELQVETYGSMDKREKVELILEQMRLCLLKEDNVSTQIIAKKISIKFFDDPAQHDLKLKFYNLMIQLNRDTSFLNTSRHYQAIAEPSRKKAALTPVDSATDDKKKLDDDKKKKDDDEKEKKTEAAAEPEVEVELTESQKKELTDKLVCAVIYCVLAPFDNEQSDMMAHLSKNKKLEDVPVYKEILRLFMSKELINFDTFNADFGLVLAENDMFKDATKHGKKCIAELKDRLIEHNIRIIAMYYSRLHIARMSELLNLPASRCEEYLSKLANSDTIRVKIDRPAGIIYFTTKKSASDILNNWATDVNQLMSLVNKTCHLINKEECVYSVMCAVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR27</accession>
    <name>A0A6I8UR27_DROPS</name>
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        <submittedName>
            <fullName evidence="6">Protein SON</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="primary">Son</name>
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    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359575.2">
        <property type="nucleotide sequence ID" value="XM_001359538.4"/>
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    </dbReference>
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    <dbReference type="InParanoid" id="A0A6I8UR27"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DSRM_SON-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dsRBD_dom"/>
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        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032922">
        <property type="entry name" value="SON"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46528">
        <property type="entry name" value="PROTEIN SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46528:SF1">
        <property type="entry name" value="PROTEIN SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="G-patch" evidence="4">
        <location>
            <begin position="689"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="777"/>
            <end position="847"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="334"/>
        </location>
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        <location>
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            <end position="419"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="739"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="534"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="582"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359575.2"/>
        </source>
    </evidence>
    <sequence length="851" mass="94352" checksum="A958F283626EC433" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URI9</accession>
    <name>A0A6I8URI9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4802970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359781.2">
        <property type="nucleotide sequence ID" value="XM_001359744.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URI9"/>
    <dbReference type="GeneID" id="4802970"/>
    <dbReference type="KEGG" id="dpo:4802970"/>
    <dbReference type="InParanoid" id="A0A6I8URI9"/>
    <dbReference type="OMA" id="NCYVRYL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070947">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032004">
        <property type="entry name" value="DUF4790"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16037">
        <property type="entry name" value="DUF4790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001359781.2"/>
        </source>
    </evidence>
    <sequence length="197" mass="23337" checksum="C8867288D5566016" modified="2020-08-12" version="1">MSFDEFDRRASHRDSLKSTKSLASILRGHTIPVRRGTHVSPARVSGLKAEVAESLTKSMSTIFRQSTMNMRYADTTLLDLEDWVLHPRKFDDDVDVSQHPSYNIMSFSYDLNCYVRYLEAKEKYDKHFHDEMQYFIDTQDRAINQFVLRRILCYNSRWPPLHSKREINRFVTKFFQLTPKQKDRLHYLMKTDLSSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US68</accession>
    <name>A0A6I8US68_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Calcium uptake protein 3, mitochondrial isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MICU3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360051.4">
        <property type="nucleotide sequence ID" value="XM_001360014.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US68"/>
    <dbReference type="SMR" id="A0A6I8US68"/>
    <dbReference type="FunCoup" id="A0A6I8US68">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="GeneID" id="4803286"/>
    <dbReference type="KEGG" id="dpo:4803286"/>
    <dbReference type="InParanoid" id="A0A6I8US68"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078343">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF13">
        <property type="entry name" value="MITOCHONDRIAL CALCIUM UPTAKE 3, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="439"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001360051.4"/>
        </source>
    </evidence>
    <sequence length="529" mass="61035" checksum="7D697AD753CEF561" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USA1</accession>
    <name>A0A6I8USA1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aromatic-L-amino-acid decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Tdc1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360140.2">
        <property type="nucleotide sequence ID" value="XM_001360103.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USA1"/>
    <dbReference type="FunCoup" id="A0A6I8USA1">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803425"/>
    <dbReference type="InParanoid" id="A0A6I8USA1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000018">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF76">
        <property type="entry name" value="FI02861P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="483"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="555"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="567"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001360140.2"/>
        </source>
    </evidence>
    <sequence length="593" mass="66164" checksum="78929242EE0CDD75" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USB8</accession>
    <name>A0A6I8USB8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4803343</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360123.1">
        <property type="nucleotide sequence ID" value="XM_001360086.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USB8"/>
    <dbReference type="FunCoup" id="A0A6I8USB8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="4803343"/>
    <dbReference type="KEGG" id="dpo:4803343"/>
    <dbReference type="InParanoid" id="A0A6I8USB8"/>
    <dbReference type="OMA" id="CLQYFPQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078394">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058698">
        <property type="entry name" value="CUB_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33236:SF12">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33236">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 122 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26080">
        <property type="entry name" value="CUB_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026302638" description="CUB domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360123.1"/>
        </source>
    </evidence>
    <sequence length="320" mass="35032" checksum="40585923DF20BDC1" modified="2020-08-12" version="1">MNDYIASTFLLLVAVAAAQAASVATPVCGGTTSSKNINLVNPTYPPRECEYQIKAYSKYVCQLRIDFAMTLAQPTLETQQSTGLTYAECTNDYFEVNGLKLCGSEVWQHIYVPFNATDGESVVDLSVVLANRAGSTGLPTPYWDMTVQQLECPAGASVRDLEVETRASTIKDGFFVAPPGCLQYFPQAKGVVKSFNYNEGAGIYPSRMNYAICFRRTETTTELNIRAYHFNVGAQESASTLLTDNSCYSSDSTSDLDADFLMVPQATLEDSHKHATYFCGSIQKDVVISTNNPGPLLVLFNSDDVYRQSEAGFAFTYTVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTN7</accession>
    <name>A0A6I8UTN7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360748.2">
        <property type="nucleotide sequence ID" value="XM_001360711.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTN7"/>
    <dbReference type="GeneID" id="4804142"/>
    <dbReference type="KEGG" id="dpo:4804142"/>
    <dbReference type="InParanoid" id="A0A6I8UTN7"/>
    <dbReference type="OMA" id="CDVTHRQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080974">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UTN7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029383">
        <property type="entry name" value="ARL6IP6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28640">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28640:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15062">
        <property type="entry name" value="ARL6IP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360748.2"/>
        </source>
    </evidence>
    <sequence length="196" mass="21375" checksum="1BB2369F064659FE" modified="2020-08-12" version="1">MKTNVAAAAVIRKSNAQQSTTRIFTSTPNNSRQRTEITSPLFRNCVGAVEAADKGGVGVGVTLTTFDPRLKKAALFLGILIIGYKSVILSMPYINTDSVQQLTGFVQGQWSEALGWSERHQLVSKLSPLLCGLIVATFAYGMVYLDSAVPGVTPPSPFSPRSKQRFRQQKSASLHLGYLCALFSGLLVTIFMYFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU71</accession>
    <name>A0A6I8UU71_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4804372</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360945.4">
        <property type="nucleotide sequence ID" value="XM_001360908.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU71"/>
    <dbReference type="FunCoup" id="A0A6I8UU71">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804372"/>
    <dbReference type="InParanoid" id="A0A6I8UU71"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10332">
        <property type="entry name" value="SLC6sbd-B0AT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF236">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="5 9" id="KW-0769">Symport</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="476"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="593"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="640"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="649"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="698"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Na(+)</name>
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        <ligand>
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        </ligand>
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        <source>
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        </source>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361046.3">
        <property type="nucleotide sequence ID" value="XM_001361009.4"/>
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    </dbReference>
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        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
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        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
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        <location>
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            <end position="676"/>
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        <location>
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        </location>
    </feature>
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            <end position="897"/>
        </location>
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        </location>
    </feature>
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            <begin position="440"/>
            <end position="458"/>
        </location>
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        <location>
            <begin position="502"/>
            <end position="511"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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        </source>
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                <consortium name="RefSeq"/>
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            <strain evidence="9">MV-25-SWS-2005</strain>
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        </source>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="MADF-dom"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
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            <end position="452"/>
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            <end position="509"/>
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        <location>
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            <end position="595"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361402.4"/>
        </source>
    </evidence>
    <sequence length="595" mass="70306" checksum="362BE6CCFB35227C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
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        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
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    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="259"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361716.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015040537.2"/>
        </source>
    </evidence>
    <sequence length="447" mass="50312" checksum="D8028F09EC039060" modified="2020-08-12" version="1">MLGRTQKLILGIAILILVDVVWVSSSELTKFLYNEAKFDKPFFCTYFKTSMFSIYLLVIGILAPWKESCERQNGTYAMMEQNVDDENYYSNQAVLGDPTYVPIRSAHLASTVNGSGNTLSGTESDDSSVRSVRFSKMAEVREMSAHEATDALLARLSYAASLRIRRQKTHHKTAKTALLFCLLWFVANYFFQLALEMDETAMITLVSSTSSFFIICMAAVFPSATGDKLTITKLIAVGMNIAGVVAITMNDLHDTKMTRGVLLALFSAFFYASYLVFVKRKSDTEEKVDIPLFFGFVGLWNLMLLWPIFFILHFTKIETFEVPDQGQFALLFLNGLIGTVLAEALWLWGCFLTSSLIGTLAMSLQIPLAIVFDVLLKHKPYSHMFYIGSIPIFLALTLVALLMRNDDADPLTKLFKIVYRKLCRCHKPSIVRVNDDEQQESLISNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWK6</accession>
    <name>A0A6I8UWK6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10">CDK-activating kinase assembly factor MAT1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">CDK7/cyclin-H assembly factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7 10">Menage a trois</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">RING finger protein MAT1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Mat1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Associates with CDK7 and cyclin H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361741.2">
        <property type="nucleotide sequence ID" value="XM_001361704.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWK6"/>
    <dbReference type="FunCoup" id="A0A6I8UWK6">
        <property type="interactions" value="1458"/>
    </dbReference>
    <dbReference type="GeneID" id="4805322"/>
    <dbReference type="KEGG" id="dpo:4805322"/>
    <dbReference type="InParanoid" id="A0A6I8UWK6"/>
    <dbReference type="OMA" id="QGLYYTA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080476">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16517">
        <property type="entry name" value="RING-HC_MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000037">
        <property type="entry name" value="Cdk-activating kinase assembly factor MAT1, centre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004575">
        <property type="entry name" value="MAT1/Tfb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057657">
        <property type="entry name" value="MAT1_CAK-anch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00570">
        <property type="entry name" value="cdk7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25811">
        <property type="entry name" value="CAK-anch_MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17121">
        <property type="entry name" value="zf-C3HC4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003338">
        <property type="entry name" value="MAT1_metazoa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="6"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="115"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR003338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001361741.2"/>
        </source>
    </evidence>
    <sequence length="320" mass="36687" checksum="EEC94EBB474A3845" modified="2020-08-12" version="1">MEDQACPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFLKGSGACPECMVPLRRNNFRVQLFEDPMVEKEVDIRRRILRDYNKREEDFGTLEEYNDYLEEIEDIVYNLCNNIEIIETNKRIEAYKRDNREVIQRNKTRVGRDESVLEEMLELERVQEESRKKELDELETEHKKKKARDKQALIEELMYSGKDAAQIVTEFAEKAEKQREEEKQLPPPKLSNEFSTGIKFGQTADPSLIPVPKSEEGPLFVYEPLIAITDGPTPPPTNEIESLGYISHIRAETQQENAGGFTATMACERALQEALQGLFYTPTAGVAVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXH4</accession>
    <name>A0A6I8UXH4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-dependent kinase 2-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132664.2">
        <property type="nucleotide sequence ID" value="XM_002132628.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXH4"/>
    <dbReference type="FunCoup" id="A0A6I8UXH4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902938"/>
    <dbReference type="InParanoid" id="A0A6I8UXH4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132664.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24898" checksum="3F31CBB94D425740" modified="2020-08-12" version="1">MSKTKKTPQKQSETDIRTTSLTPVRYLDSPRLQSPTSHDATLATCKTRLETLVKQLQDNYAKWQLAQQRGTAICYAIEAKKTKCLEKSEDDDSISTAYPDDLILPCNKLAIIASIFGDIANNTRETLRQLRALTKLPGAAADTIFYRSWRLSQFVAFTRELAERYEEEALVKIQVSENIAHCTRRSDLIAHTSLWEYPEHVDAYVKLGFLLLAEELSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXS0</accession>
    <name>A0A6I8UXS0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Kinetochore and Eb1-associated basic protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Kebab</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002132849.3">
        <property type="nucleotide sequence ID" value="XM_002132813.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXS0"/>
    <dbReference type="FunCoup" id="A0A6I8UXS0">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903149"/>
    <dbReference type="InParanoid" id="A0A6I8UXS0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0137">Centromere</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="391"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002132849.3"/>
        </source>
    </evidence>
    <sequence length="596" mass="68767" checksum="EF1EBE311B31AA7E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY81</accession>
    <name>A0A6I8UY81_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6902649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133165.2">
        <property type="nucleotide sequence ID" value="XM_002133129.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY81"/>
    <dbReference type="KEGG" id="dpo:6902649"/>
    <dbReference type="InParanoid" id="A0A6I8UY81"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133165.2"/>
        </source>
    </evidence>
    <sequence length="438" mass="50608" checksum="87CD5C9CF3DD9315" modified="2020-08-12" version="1">MSEQAAGSNVEKADLEGNRKRKADGQYTSGTVIAEELKMRTSRTSILDVNEDCYRFMMDYLNVRDQLSLASSHLYIGEMFRRYASHRYKNINESLTSRTSDENLKQLLQIVGEDLVSYESESNRSSTGDQHLWLLRSLRSHCTKLKHLKMTFGRTRGGWDDLYSLKQLKSLHAELHMADRWCSDFVYNLKQFPCLRKLTLSAVSYSGRDLHVLDQLEYLDVDYCDGLDGKSLADCCQTMKQLRHLDFGCLRNFSKSTFESLLTNCQQLECLVFDVGHLDSTVPYEMLCHLPRLRYLNVWTCYLRDSFIEGLMNKQGSPLESLFLEGCALSADQIKHLCNISTLTELRVSCDTVPLEDLLKLKSLLNLHISMPITTGQIVDLLQGLPRLKFLNILDRDDVDKVNLGRSVHTWMNEQREQRRKIKVCFSNTYTDDILWLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYN9</accession>
    <name>A0A6I8UYN9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6902096</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_002133627.2">
        <property type="nucleotide sequence ID" value="XM_002133591.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYN9"/>
    <dbReference type="KEGG" id="dpo:6902096"/>
    <dbReference type="InParanoid" id="A0A6I8UYN9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF83">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002133627.2"/>
        </source>
    </evidence>
    <sequence length="584" mass="68985" checksum="8ABEDBDB77CC84A7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZA4</accession>
    <name>A0A6I8UZA4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ARL-6-interacting protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6901263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133964.3">
        <property type="nucleotide sequence ID" value="XM_002133928.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZA4"/>
    <dbReference type="KEGG" id="dpo:6901263"/>
    <dbReference type="InParanoid" id="A0A6I8UZA4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZA4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052114">
        <property type="entry name" value="ER_autophagy_membrane_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952:SF0">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952">
        <property type="entry name" value="ADP-RIBOSYLATION-LIKE FACTOR 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002133964.3"/>
        </source>
    </evidence>
    <sequence length="192" mass="21890" checksum="9B98670415784A1C" modified="2020-08-12" version="1">MSKTPIELDTQRSTEMTASDIGNNAMVIKLKHDLERFQAPIVSAYGVLTWEKKCYPVVVLGGISVTFFVLWYMDLSVITLMSLLALLTTISHYFLLWCLDLPDFILDFFQTKIDEAKFESVCGQMYIIKEGLAAWYQYLFKERKSTGLVIVKSLVLLAMAWIGSIVNNLLLMYLVTLLIAMWAGLQAKHVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZP6</accession>
    <name>A0A6I8UZP6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCCH domain-containing protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134269.2">
        <property type="nucleotide sequence ID" value="XM_002134233.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZP6"/>
    <dbReference type="FunCoup" id="A0A6I8UZP6">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901598"/>
    <dbReference type="InParanoid" id="A0A6I8UZP6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="CDD" id="cd00934">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41148">
        <property type="entry name" value="LP09875P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41148:SF1">
        <property type="entry name" value="LP09875P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="2">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="491"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="582"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="745"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="777"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="919"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="955"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002134269.2"/>
        </source>
    </evidence>
    <sequence length="1008" mass="114831" checksum="6903D9C964CC4D2A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZZ6</accession>
    <name>A0A6I8UZZ6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="11">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC6901774</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable phosphatase which plays a redundant role with gsp-4 in spermatogenesis by regulating sister chromatid segregation during meiosis. In addition, involved in sperm motility by controlling the dynamic disassembly of major sperm proteins (MSP) in the spermatozoan pseudopodium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 16">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Pseudopodium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="11"/>
    <dbReference type="RefSeq" id="XP_002134444.3">
        <property type="nucleotide sequence ID" value="XM_002134408.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZZ6"/>
    <dbReference type="FunCoup" id="A0A6I8UZZ6">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901774"/>
    <dbReference type="InParanoid" id="A0A6I8UZZ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZZ6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031143">
        <property type="term" value="C:pseudopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097723">
        <property type="term" value="P:amoeboid sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018991">
        <property type="term" value="P:egg-laying behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007060">
        <property type="term" value="P:male meiosis chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031272">
        <property type="term" value="P:regulation of pseudopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="13">
        <location>
            <begin position="149"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002134444.3"/>
        </source>
    </evidence>
    <sequence length="330" mass="37657" checksum="271D602448E75CBB" modified="2020-08-12" version="1">MPKTILFCISLQIERFKKPSYTLNIRGNSSIKMILDETKLDQIIQELTGLTGGENCTLSKKVIVALCREAREVFLNQPMLLELDVPIKVCGDVHGQFTDLLRVFKACGFPPQTNYLFLGDYVDRGKQSLETICLLMAYKVRYPENFFLLRGNHECAGITRLYGFYDEIKRRHNVRTWREFTDCFDCFPAAAVVANRIFCCHGGLSPSLESLDQIRNLPRPTDIPKEGLLCDLLWADLNRTGLGWGHNDRGVSFTYDEKIITDFLAAYNFDLMIRAHEVVEDGYEMFADRRLISIFTAPNYCGLWNNAAAVMCVSAELVVSFVILKRKICN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2J0</accession>
    <name>A0A6I8V2J0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137283.2">
        <property type="nucleotide sequence ID" value="XM_002137247.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2J0"/>
    <dbReference type="KEGG" id="dpo:6897099"/>
    <dbReference type="InParanoid" id="A0A6I8V2J0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026090902" description="Kazal-like domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="2">
        <location>
            <begin position="87"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137283.2"/>
        </source>
    </evidence>
    <sequence length="144" mass="16519" checksum="D426832CBF3C1DDF" modified="2020-08-12" version="1">MHRTMELLKILSLGILLTVLGCISAEEYSLFDCSVKYKCSGEKALVWATADERCFVFHNKCLMRVEQCARSFSGRSELIETDRETCKPTCTKDCLDLYEPVCAQIFQEEYITFSNECEMRNNLCENERPYSYFAIGECVESPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V303</accession>
    <name>A0A6I8V303_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6897567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137712.2">
        <property type="nucleotide sequence ID" value="XM_002137676.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V303"/>
    <dbReference type="GeneID" id="6897567"/>
    <dbReference type="KEGG" id="dpo:6897567"/>
    <dbReference type="InParanoid" id="A0A6I8V303"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247754">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029138">
        <property type="entry name" value="SNAPC5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15497">
        <property type="entry name" value="SNAPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002137712.2"/>
        </source>
    </evidence>
    <sequence length="117" mass="13362" checksum="07691C8C5D749365" modified="2020-08-12" version="1">MWGCMCTDINNRTTTMLQSNRVLQREQVELLAIEKKLLKVVPVIQEALNSLKVEELHLKSLGLERTIESQRVVPAHRTHFDMNAPPAAMQPDQINQQKIDLDLFINKLEGGEELDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V304</accession>
    <name>A0A6I8V304_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Or85e</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137585.2">
        <property type="nucleotide sequence ID" value="XM_002137549.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V304"/>
    <dbReference type="FunCoup" id="A0A6I8V304">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802030"/>
    <dbReference type="InParanoid" id="A0A6I8V304"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF42">
        <property type="entry name" value="ODORANT RECEPTOR 83A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002137585.2"/>
        </source>
    </evidence>
    <sequence length="467" mass="52757" checksum="1DA7DF18FA6B39B6" modified="2020-08-12" version="1">MASLQFHGNIDADTRYDATLDPAREPELFRLLMGLQLAMGMNPSPRLPSWWPTWLRPVGGLMAKAYCSMVILTSLHLGLLFTKTTLDVLPTGELQAITDALTMTIIYFFTAYANIYWCVRSQRLLAFMDHINREYRHHSLAGVTFVSSHAAHRWSRSFTTIWILSCLVGVITWGVSPLMLGIRTLPLTCWYPFDALSPGTYTAVYATQLFGQISVGVTFGFGGSLFVTLCLLLLAQFDVLFCSLKNLDAHSKLLSGETIAGLGLLQRELLQGAFTRELNQYAVLQEHATDLLRISAESQNLAQVKVFHSALVECVRLHRFILYCCAELENLFSPYCLVKSMQITLQLCLLVFVGVSGTREFLRIVNQIQYLALTLFELLMFTYCGELLSRHSVRSGEAFWRGGWWKHAHLLRQDVLIFLVNSRRAVYVTAGKFYVMDVNRLRSVITQAFSFLTLLQKLAAKKATMEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V394</accession>
    <name>A0A6I8V394_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc finger protein 664 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6897605</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137750.2">
        <property type="nucleotide sequence ID" value="XM_002137714.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V394"/>
    <dbReference type="KEGG" id="dpo:6897605"/>
    <dbReference type="InParanoid" id="A0A6I8V394"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF66">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="10">
        <location>
            <begin position="3"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="368"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002137750.2"/>
        </source>
    </evidence>
    <sequence length="399" mass="47182" checksum="C8623EBEB64DAA1A" modified="2020-08-12" version="1">MAELCRICRSHSVTLFGIFDERERRQWEGEMEPILADMVMACADVKIEAGDELSQNICLSCILNAQSSYRFKRRCERSHEESYLAIQTQSHQELLSIKMEPMLDTIEFEEESFIVEDMFDAETEVKLKLEEPIVEQKSPKIEVEEKRQKRLTSGARKQLQETNKEAFKCDLCIKQFQRKRNLDEHMKIHTRSHICQTCEQRFLFKTDLDVHMSLHKSNRPYQCPLCMKSFATSQNRTNHKCLLEEDRPFKCTHCPQTFVHNYYLKEHMLSHTEEESNKPGTGPHKCTHCEIGFHNKTALKIHTLSHTGERPHTCAFCASSFRSKQTLKIHIRIHTGEKPYKCPECQHAFADNKNLCKHRRRHTDDRPYKCPHCVMAFREKHHMKRHVLRKHRTSENDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3E3</accession>
    <name>A0A6I8V3E3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">VPS35 endosomal protein sorting factor-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6897888</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS35L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137990.2">
        <property type="nucleotide sequence ID" value="XM_002137954.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3E3"/>
    <dbReference type="FunCoup" id="A0A6I8V3E3">
        <property type="interactions" value="2295"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897888"/>
    <dbReference type="InParanoid" id="A0A6I8V3E3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V3E3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029705">
        <property type="entry name" value="VPS35L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13673">
        <property type="entry name" value="ESOPHAGEAL CANCER ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13673:SF0">
        <property type="entry name" value="VPS35 ENDOSOMAL PROTEIN-SORTING FACTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002137990.2"/>
        </source>
    </evidence>
    <sequence length="942" mass="107788" checksum="707958E203116845" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3P6</accession>
    <name>A0A6I8V3P6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sodium channel protein Nach</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ppk25</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138176.2">
        <property type="nucleotide sequence ID" value="XM_002138140.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3P6"/>
    <dbReference type="KEGG" id="dpo:6898094"/>
    <dbReference type="InParanoid" id="A0A6I8V3P6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF240">
        <property type="entry name" value="PICKPOCKET 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="410"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002138176.2"/>
        </source>
    </evidence>
    <sequence length="468" mass="54924" checksum="FC909447143C77EA" modified="2020-08-12" version="1">MEHQEDSKEKCRSHDWWAEFCQDSAVHGMPYLARRDLHWVERLFWLAMILASAYYAISSCYSQWERFRNNPIVYEYEYLFALRNFTFIGLTFCTTYETEEQVAKLIRDTWAVDPTQDPAKAAYYKEFLYVLNRLRYNNLETLKPFENDTTLSNLAYVDMLLALQEKVLPSPRPVLMAPIVTEVGLCQSTSQLTRYGNPYGRLETLNVTPVRQCGFFNECQFMHKPFNSIQTHVFLYMHDVNELMLPHDRRTITFDAKTKSSYVLKLLISSISADREVRNLPVAYRKCRYHDENNLRYYSPYHPSLCRLECRINWALSMCHCKPFFYAAAPQAPICNVTGMLCLAESNWLERPCHCFSLCRETTYSIIEEYEQSGGDQSYVGEQFERTIIIKLELPKMGMKRRVVFSTDQLIMSFGGAIGLFLGASFMTFYGLIHLLLHFLVVQCKCRPRIPQDRAPDGKRKWFTFRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4C8</accession>
    <name>A0A6I8V4C8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Acyl-CoA Delta(11) desaturase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6897735</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The histidine box domains are involved in binding the catalytic metal ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137841.2">
        <property type="nucleotide sequence ID" value="XM_002137805.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4C8"/>
    <dbReference type="KEGG" id="dpo:6897735"/>
    <dbReference type="InParanoid" id="A0A6I8V4C8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001522">
        <property type="entry name" value="FADS-1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00476">
        <property type="entry name" value="FATTY_ACID_DESATUR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="15">
        <location>
            <begin position="75"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002137841.2"/>
        </source>
    </evidence>
    <sequence length="361" mass="41675" checksum="1A0E3F0E27B10092" modified="2020-08-12" version="1">MPPNSDVIELGPSPDTTDVLCETDAGPKGTASDLSTLKTTDGRKLELVWINIILFIYVHTASLYGIWLMLTTTKWQTDVFAVILFSLASLGITAGAHRLWAHRTYKANVAVRLILLFFNTLAFQDAVYYWARDHRLHHKCTDTDADPYNSKRGWWFAHIGWLCCKKHPDVTAKGKLIDLSDLQRDPLVMFQKKYYLILMPILCFILPTLVPVYFWGESLSVAWHVPALTRWCLMLNVVWLLNSSGHMHGKRPYDRSISPTSLVFLIWLRYGEGYHNYHHVFPWDYKGAEMGHYSQDLPTILIRMFARLGWAYDLKSVSMDMVRKRVLRTGDGTHPVWGWGDKEHPQEDIDSTTITYKKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4L7</accession>
    <name>A0A6I8V4L7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mitochondrial amidoxime-reducing component 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138693.1">
        <property type="nucleotide sequence ID" value="XM_002138657.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4L7"/>
    <dbReference type="FunCoup" id="A0A6I8V4L7">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="GeneID" id="6898688"/>
    <dbReference type="KEGG" id="dpo:6898688"/>
    <dbReference type="InParanoid" id="A0A6I8V4L7"/>
    <dbReference type="OMA" id="CDEHEPY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246313">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF19">
        <property type="entry name" value="MITOCHONDRIAL AMIDOXIME REDUCING COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="MOSC" evidence="2">
        <location>
            <begin position="180"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138693.1"/>
        </source>
    </evidence>
    <sequence length="339" mass="38360" checksum="E385C495DCD0CA8F" modified="2020-08-12" version="1">MAYSSSSLDISPKTLIGVGVGLAVAGGASYFLYHRYLQYRDAMPQKWRRVGTLQRINLFPIKSCAPLDSTPGREYDCDVLGMSIDGIRDRTLMLLDENNTMVTARGYPHMKLIKPWQVSDNGLIITAPEMPELELDFKNLESPGQDLRTSIWGAPVDAKLCGDRLNKWFSQFILEKNTGLKLVHYPYPKPVKIICDDLKNMPFMRQEDSGTFNDGTSYMLMNLSSVDDLNTRLKNPVQPLQFRGGFEIKCDEHEPYAEDNWQWVRIGDEAVFRQVAPRTLCIVPNVNDNTGKRDVEGEPLKTLKSYRTFNHSSPLLGIHLGLRQPGKVKANDVVYVGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4P8</accession>
    <name>A0A6I8V4P8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6898819</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138793.2">
        <property type="nucleotide sequence ID" value="XM_002138757.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4P8"/>
    <dbReference type="FunCoup" id="A0A6I8V4P8">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898819"/>
    <dbReference type="InParanoid" id="A0A6I8V4P8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17336">
        <property type="entry name" value="MFS_SLCO_OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF76">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="537"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="576"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="458"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002138793.2"/>
        </source>
    </evidence>
    <sequence length="689" mass="75846" checksum="0B3051C306B03CE3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V503</accession>
    <name>A0A6I8V503_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Vps35</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138795.1">
        <property type="nucleotide sequence ID" value="XM_002138759.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V503"/>
    <dbReference type="SMR" id="A0A6I8V503"/>
    <dbReference type="FunCoup" id="A0A6I8V503">
        <property type="interactions" value="2670"/>
    </dbReference>
    <dbReference type="GeneID" id="6898822"/>
    <dbReference type="KEGG" id="dpo:6898822"/>
    <dbReference type="InParanoid" id="A0A6I8V503"/>
    <dbReference type="OMA" id="YIRSREY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245592">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V503">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000005">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002138795.1"/>
        </source>
    </evidence>
    <sequence length="822" mass="93148" checksum="DF3710FC4D83B2A7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V662</accession>
    <name>A0A6I8V662_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Decapping nuclease</fullName>
            <ecNumber evidence="2">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cuff</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DXO/Dom3Z family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="2"/>
    <dbReference type="RefSeq" id="XP_002138912.3">
        <property type="nucleotide sequence ID" value="XM_002138876.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V662"/>
    <dbReference type="FunCoup" id="A0A6I8V662">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898951"/>
    <dbReference type="InParanoid" id="A0A6I8V662"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V662">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013961">
        <property type="entry name" value="RAI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039039">
        <property type="entry name" value="RAI1-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395:SF9">
        <property type="entry name" value="DECAPPING AND EXORIBONUCLEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395">
        <property type="entry name" value="DOM-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08652">
        <property type="entry name" value="RAI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RAI1-like" evidence="3">
        <location>
            <begin position="35"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002138912.3"/>
        </source>
    </evidence>
    <sequence length="382" mass="43964" checksum="EED8274C8CA44FFF" modified="2020-08-12" version="1">MFANESMMLSDEILLNIKPHVITKADRCDFPEFSKPVAHGGFSLSPYGKFENNANRLRYVVVPKMDKFQVHLGDSEPSPPRSLLLSQPGLDNILLYMESTGTSHLSAKLEVEVSYDIVSTTEVLELILRTPYEIKENWTLAVTKYRNTIYISPVVPEDLKTSPMKELNADWLAKLRRHFLSENANAMPKPCDPMDPTGQYNGVFSFSINGTRFIFDSPVLAEGFCTGSDRSSNYVFTELQFRQDSMSVDEWATHNRCDALKWWSKCFLVGIENIYVANVNRHTIAHTVNKLSVRQLWKDCEKAWSPHVCVNFLLRLVDQIRGFMSVVDCPRTVYLMKFDASRGEISYTSYLGRNLYTFIPDWFRIMLDERMEDFTPTTLHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V809</accession>
    <name>A0A6I8V809_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Ndae1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035785.2">
        <property type="nucleotide sequence ID" value="XM_015180299.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V809"/>
    <dbReference type="FunCoup" id="A0A6I8V809">
        <property type="interactions" value="545"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V809"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V809">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003024">
        <property type="entry name" value="Na/HCO3_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF36">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01232">
        <property type="entry name" value="NAHCO3TRSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="649"/>
            <end position="676"/>
        </location>
    </feature>
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</entry>
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        <source>
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</entry>
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        <name evidence="3 4" type="primary">LOC26534429</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <scope>IDENTIFICATION</scope>
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            <end position="78"/>
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            <begin position="111"/>
            <end position="131"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="410"/>
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    </feature>
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        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015041686.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015041687.2"/>
        </source>
    </evidence>
    <sequence length="683" mass="74173" checksum="B9B9047556D4CCCC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VKC0</accession>
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        <submittedName>
            <fullName evidence="7">Collagen alpha-1(XVIII) chain isoform X1</fullName>
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        <name evidence="7" type="primary">Mp</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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    </dbReference>
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        <property type="entry name" value="MULTIPLEXIN, ISOFORM R"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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</entry>
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    <accession>A0A6I8VKE9</accession>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
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                <consortium name="RefSeq"/>
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            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
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        <location>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015043163.2"/>
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    <sequence length="196" mass="22574" checksum="8B5DFFD770F020C7" modified="2020-08-12" version="1">MADQPIKVQRDYLKIVCYAIAAFEALHSLYFLLDTAYLLISYTNLYTIMAFLGTNVWVLTVVALSVGLWKERPMLFIFWFFFSGIGTITDIVYMVWNVTASLTFDLAHFLRWTILYCGIVLECTGLYLVYSYYQRLNRQKNDDSASRPKDSALRPKDSASRPKNSASRPKRRKRYVPKSPALQSGTTSYPATDTKR</sequence>
</entry>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <strain evidence="9 10">MV-25-SWS-2005</strain>
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        <text evidence="1">Homodimer; disulfide-linked.</text>
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        <property type="entry name" value="PROTEIN SPAETZLE 5"/>
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    </dbReference>
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        <property type="entry name" value="Cystine-knot cytokines"/>
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    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634531" evidence="6">
        <location>
            <begin position="32"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Spaetzle" evidence="7">
        <location>
            <begin position="300"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="220"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001352716.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015043778.2"/>
        </source>
    </evidence>
    <sequence length="396" mass="44017" checksum="6DE29FC0B7E0F4DA" modified="2020-08-12" version="1">MTKSIKCLRTSSCNRILLAYVILACTAAAHSSPPPCGLYGAPPCQFLPAPPGQTPSCARPGKTYCEHADNYPTYLIKSLVRKWGYEAGTLLVDETWEDFAAVAWHETPVFYDPKSIFPSRDPNGQDFNGYSYQTPFGGSSSQRVNGGNSLFASSPSTEAPTYLLYTSSGGGHRAGQRYNHHQGAAGGSAGQLFFNQAGKTTPYNATLWLKRLVRDLSRKQKEEVPPEEEKQPEKEEELLEKEEEQQPKEGEEQQQQEEEEHQQIKRDVSLNMDLLDIVGVAGNPSKKRTRTKRQSPGRSTLCQTTSQFITPQAALNSRGNWMFVVNEQNTARQMVKAELCASNTCSNLCELPNGYNSRCEQKFVQKRLIALQGNGQNLYTDTFWFPSCCVCTIAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMS8</accession>
    <name>A0A6I8VMS8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6896813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232366.1">
        <property type="nucleotide sequence ID" value="XM_033376475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VMS8"/>
    <dbReference type="KEGG" id="dpo:6896813"/>
    <dbReference type="InParanoid" id="A0A6I8VMS8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026034513" description="MD-2-related lipid-recognition domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20703" checksum="0F655E9ABBCCAC92" modified="2020-08-12" version="1">MDYKLISFGIICLTIFLIGSEAPSVKLTNAKCESYNKSWVIMHTCRLKAHSRNKTSLNVNLTVLEPAYKISAKFKMWKRANGYKPFLFDFHLDCCEFLRRRNHPVGKIIYNLLKDSSTVNHSCPYMGVQTLSDFHRIELPVVLPSGEYLVTFIWMFDGKPQFSSNISFTYLEDLWTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VN35</accession>
    <name>A0A6I8VN35_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MICOS complex subunit MIC10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC26532160</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion inner membrane</location>
            <topology evidence="2 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015044553.2">
        <property type="nucleotide sequence ID" value="XM_015189067.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VN35"/>
    <dbReference type="FunCoup" id="A0A6I8VN35">
        <property type="interactions" value="477"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532160"/>
    <dbReference type="InParanoid" id="A0A6I8VN35"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271147">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VN35">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304:SF0">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015044553.2"/>
        </source>
    </evidence>
    <sequence length="73" mass="8015" checksum="01ADA3D51F2942C9" modified="2020-08-12" version="1">MPVEEKVFLEDNVGKRLDRCVSDTIVKGGGGLIIGSAVSLLFFRRRAWPAWLGTGFGIGVAYRTCEKDLNSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNK7</accession>
    <name>A0A6I8VNK7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Extensin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Muc91C</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232612.1">
        <property type="nucleotide sequence ID" value="XM_033376721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNK7"/>
    <dbReference type="KEGG" id="dpo:6897152"/>
    <dbReference type="InParanoid" id="A0A6I8VNK7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032059">
        <property type="entry name" value="Mucin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15363:SF4">
        <property type="entry name" value="CUTICLE PROTEIN 16.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15363">
        <property type="entry name" value="POU DOMAIN CLASS 2-ASSOCIATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16058">
        <property type="entry name" value="Mucin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026285566" evidence="2">
        <location>
            <begin position="22"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="Mucin-like" evidence="3">
        <location>
            <begin position="195"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Mucin-like" evidence="3">
        <location>
            <begin position="300"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Mucin-like" evidence="3">
        <location>
            <begin position="542"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Mucin-like" evidence="3">
        <location>
            <begin position="647"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="245"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="369"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="846"/>
            <end position="865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232612.1"/>
        </source>
    </evidence>
    <sequence length="876" mass="83614" checksum="43F5E056802AE5F6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VP29</accession>
    <name>A0A6I8VP29_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">LOW QUALITY PROTEIN: thymus-specific serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4803256</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232816.1">
        <property type="nucleotide sequence ID" value="XM_033376925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VP29"/>
    <dbReference type="KEGG" id="dpo:4803256"/>
    <dbReference type="InParanoid" id="A0A6I8VP29"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF5">
        <property type="entry name" value="RE36938P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026242982" evidence="6">
        <location>
            <begin position="18"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033232816.1"/>
        </source>
    </evidence>
    <sequence length="486" mass="54633" checksum="21EF9BA2DBE31AB2" modified="2020-08-12" version="1">MKYTLIVLALLAPLTAAASLGNLELQPEPNAFVQSLRELRRAPPLEKSRKRANVETRWFTLKLDNFNAANNATWKDRLLINEDHFTDGSPIFIYLGGEWEIEPSAITSGLWVDIAKEHNGSLIYTEHRFFGKSFPITECFFETNLKYQSVQQALADVVHIIKILKLEDKYKDSKVIVSGCSYSATMATLIRKLYPDIILGSWASSAPLEAKVDFKDYMEVVGQAFEQLGGKYCYDLIDNATSYYQDLFEGGQGAKAKKELNLCANFNVNSKQDRWQIFSTIANVFAGLAQYQKPGNYDLPKYCSVLRSFSDDDAEALSKFVQWRLGYPTCLSVTYKGTVNYYKWAKINYEDDSGLPWIYQTCSEFGWYQSSDSENQPFGSSFPATLYTDTCHDVFSKNYTLINIEANIAATNKDFQGIDIAVKNVYWTQGGLDPWSKVGAGIAQGATIIPQASHCSDLDSISANDSPELQASKLKLAQLVRDWLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPL5</accession>
    <name>A0A6I8VPL5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Fasciclin-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">Fas1</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037269.1">
        <property type="nucleotide sequence ID" value="XM_015181783.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232981.1">
        <property type="nucleotide sequence ID" value="XM_033377090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPL5"/>
    <dbReference type="GeneID" id="4801486"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079701">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VPL5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000039">
        <property type="entry name" value="Fasciclin 1, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000038">
        <property type="entry name" value="fasciclin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000035">
        <property type="entry name" value="fasciclin-1 isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF80">
        <property type="entry name" value="FASCICLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634538" evidence="1">
        <location>
            <begin position="24"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="2">
        <location>
            <begin position="23"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="2">
        <location>
            <begin position="182"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="2">
        <location>
            <begin position="342"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="2">
        <location>
            <begin position="492"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015037269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232981.1"/>
        </source>
    </evidence>
    <sequence length="689" mass="77440" checksum="F4BBF42A3BBD3E0F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPX9</accession>
    <name>A0A6I8VPX9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">U-actitoxin-Avd3s-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC117183351</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232923.1">
        <property type="nucleotide sequence ID" value="XM_033377032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPX9"/>
    <dbReference type="KEGG" id="dpo:117183351"/>
    <dbReference type="InParanoid" id="A0A6I8VPX9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VPX9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026302139" evidence="3">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="4">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033232923.1"/>
        </source>
    </evidence>
    <sequence length="93" mass="10698" checksum="3CAAF1284BF5123F" modified="2020-08-12" version="1">MVKLILNIVILSLLQCLALALSPHIIFYRNKLCTEPLSYGSCRSIQKRWYHDPRSKSCKVFYYSGCGGNYNRFISKTVCMEYCSNPSSPYSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQV7</accession>
    <name>A0A6I8VQV7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233460.1">
        <property type="nucleotide sequence ID" value="XM_033377569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQV7"/>
    <dbReference type="KEGG" id="dpo:6898538"/>
    <dbReference type="InParanoid" id="A0A6I8VQV7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246237">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026037277" evidence="1">
        <location>
            <begin position="25"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233460.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="22534" checksum="F883D41D729AC360" modified="2020-08-12" version="1">MATSKSSLLISLIVFGFAVSGVSCKVEFTNLKCTTLDREFLEFDKCFLKSINRTYKYLSVSTKIHMLPLKSVSVRRLLHLVFDRSHHTVPFQIQTQVLQRLNGYKPFLFNFTTDGCKALSGKMNPTTRFFFGLIAPYSNLNHSCPYDVSFCHTSLHSIDGVSYIFHSTTYLWRSFRLALWTSNSPKFCRSRRVITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VU99</accession>
    <name>A0A6I8VU99_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Myocyte-specific enhancer factor 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Mef2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033234214.1">
        <property type="nucleotide sequence ID" value="XM_033378323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VU99"/>
    <dbReference type="FunCoup" id="A0A6I8VU99">
        <property type="interactions" value="944"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804715"/>
    <dbReference type="InParanoid" id="A0A6I8VU99"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VU99">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000001">
        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022102">
        <property type="entry name" value="HJURP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF534">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12347">
        <property type="entry name" value="HJURP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="11">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="341"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="518"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="423"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="520"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033234214.1"/>
        </source>
    </evidence>
    <sequence length="562" mass="59944" checksum="191C291BEE02FB9D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWX2</accession>
    <name>A0A6I8VWX2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">3,4-dihydroxyphenylacetaldehyde synthase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC117183959</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235580.1">
        <property type="nucleotide sequence ID" value="XM_033379689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWX2"/>
    <dbReference type="KEGG" id="dpo:117183959"/>
    <dbReference type="InParanoid" id="A0A6I8VWX2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004058">
        <property type="term" value="F:aromatic-L-amino-acid decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF60">
        <property type="entry name" value="3,4-DIHYDROXYPHENYLACETALDEHYDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033235580.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="46163" checksum="146A50E349E5AF0B" modified="2020-08-12" version="1">MHAKQTLLIRICSPACTELEVVVMDWLAKFLKLSEHFQHASDGPGGGVIQGSASEAVLVAVLAAREQAVISCKETHPELSESEVRGKLIAYSSDQSNSCIEKAGVLAAMPIKLLPADDDLILRGNTLRKAIEDDVAAGLIPVICIATLGTTGTCAYDDIDSLADVCQALNVWLHVDAAYAGGGFALEECTELRRGLDRVDSLNFNLHKFMLVNFDCSAMWLKDANKVVDSFNVDRIYLKHKHEGQSQIPDFRHWQIPLGRRFRALKVWITFRTLGAEGLRSHVRKHIALAQQFEAFVRNDSRFEMVAPQALGLVCFRPRGDNEHTAQLLQRLMERKKIYMVKAEHAGRQFLRFAVCGMDAKPSDIEFAWTEIETQLTALLAEKSLAARKPSNVSELTQHFQMHLASDSGTHEKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYN5</accession>
    <name>A0A6I8VYN5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Restin homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">CLIP-190</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033236209.1">
        <property type="nucleotide sequence ID" value="XM_033380318.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236210.1">
        <property type="nucleotide sequence ID" value="XM_033380319.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VYN5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032108">
        <property type="entry name" value="CLIP1_ZNF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF93">
        <property type="entry name" value="RESTIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16641">
        <property type="entry name" value="CLIP1_ZNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="222"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="9">
        <location>
            <begin position="338"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="389"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1650"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="469"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="577"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1045"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1587"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="402"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033236209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033236210.1"/>
        </source>
    </evidence>
    <sequence length="1706" mass="192103" checksum="9853E82188C21CF3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYP5</accession>
    <name>A0A6I8VYP5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Desi</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Desi</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236221.1">
        <property type="nucleotide sequence ID" value="XM_033380330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYP5"/>
    <dbReference type="FunCoup" id="A0A6I8VYP5">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903806"/>
    <dbReference type="InParanoid" id="A0A6I8VYP5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006631">
        <property type="entry name" value="DM4_12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07841">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026014442" evidence="1">
        <location>
            <begin position="24"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236221.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="28629" checksum="1AEA8E4BEE113871" modified="2020-08-12" version="1">MNLNCVVVLQILCMMVILSNTNAIPKYDEGQKFNSEKIQRNRSYYNENKTQLNEPQTASTKDRLERLGYTTGYGSLNGYPGGTGLSAYNPIKLDLGGVVLGTLVGIGAIILIPKILSAFHGGYGGYGRSEDNDLTSLSTMISKIDDVLSQNNIDSTSCMQRTVCGYVRSTEYNMKGGSSDQMDEFIHMLSENVLVDYLLDGTAIKEALEIGKRENDKPCEEVYSNCPLDSKSASNILLKLLPKKNSHGKRTPAVNFGEKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0V4</accession>
    <accession>Q29MK3</accession>
    <name>A0A6I8W0V4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">MKRN2 opposite strand protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC4817040</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356626.1">
        <property type="nucleotide sequence ID" value="XM_001356590.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236971.1">
        <property type="nucleotide sequence ID" value="XM_033381080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0V4"/>
    <dbReference type="GeneID" id="4817040"/>
    <dbReference type="KEGG" id="dpo:4817040"/>
    <dbReference type="OMA" id="PNMYGMM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082134">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W0V4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032016">
        <property type="entry name" value="MKRN2OS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053921">
        <property type="entry name" value="MKRN2OS-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053922">
        <property type="entry name" value="MKRN2OS-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33963">
        <property type="entry name" value="MKRN2 OPPOSITE STRAND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33963:SF2">
        <property type="entry name" value="MKRN2 OPPOSITE STRAND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16044">
        <property type="entry name" value="DUF4796_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22795">
        <property type="entry name" value="DUF4796_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MKRN2 opposite strand protein-like N-terminal" evidence="3">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="MKRN2 opposite strand protein-like C-terminal" evidence="2">
        <location>
            <begin position="75"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033236971.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="33696" checksum="0DB5EE27DFE8820D" modified="2020-08-12" version="1">MSATTSATTITSDPGILCFHHCNAKVFCFTLPHTCPHCNAPLDAATDADEDTDADADAQSVVDMGATAVQSRLLPFRLPYPFVRATQHPCAIVLRPSTGDFLNDYSNATDLHIAVTTSGADIVEFDREGLRRHRRDDNPREWRQSLLVGDVPEPWHDYWDEVLQQICAQSTRWSITSYEETSHNCYAFVLAFLQALGHGQLSEAARSKTAFCERYIVPRTTTAGKYISLYRRLRRHGVHVHRQQPRLRTRLKSSSHTAAAPASSADVCDGARSTGKAPANSGPFSSNSIGIIGSRPRPKLCTIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W155</accession>
    <accession>Q29LN6</accession>
    <name>A0A6I8W155_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenosine 5'-monophosphoramidase HINT3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Histidine triad nucleotide-binding protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">LOC4817528</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine 5'-phosphoramidate + H2O = NH4(+) + AMP</text>
            <dbReference type="Rhea" id="RHEA:67916"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57890"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HINT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356943.2">
        <property type="nucleotide sequence ID" value="XM_001356907.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237008.1">
        <property type="nucleotide sequence ID" value="XM_033381117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W155"/>
    <dbReference type="SMR" id="A0A6I8W155"/>
    <dbReference type="GeneID" id="4817528"/>
    <dbReference type="KEGG" id="dpo:4817528"/>
    <dbReference type="OMA" id="PSFWFKK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073708">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01278">
        <property type="entry name" value="aprataxin_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF5">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HINT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="8">
        <location>
            <begin position="47"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="7">
        <location>
            <begin position="140"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356943.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033237008.1"/>
        </source>
    </evidence>
    <sequence length="181" mass="20340" checksum="DE8C74FB4030A5E5" modified="2020-08-12" version="1">MHGENFLFKRKKTIFAALTGLGLLGLVAVYNCGINSPTTSASQQECLFCDFANGKQGPPPVLEVQTDEYVIFKDKSPAATYHYLAIPKQHFDSFNALNKSHIGLVLRMESGMVELLKSKNVDPSKAIIGFHIPPFISVKHLHLHGIYPPSEMSIWNRINFMSSFWFKTARDANEALELREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-10-07" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1T2</accession>
    <name>A0A6I8W1T2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein axonemal assembly factor 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Defective transmitter-recycling protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Dynein regulatory complex subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Leucine-rich repeat-containing protein 50 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Ppr-Y</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium-specific protein required for cilia structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the DRC3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033237248.1">
        <property type="nucleotide sequence ID" value="XM_033381357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W1T2"/>
    <dbReference type="FunCoup" id="A0A6I8W1T2">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899335"/>
    <dbReference type="InParanoid" id="A0A6I8W1T2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF12">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033237248.1"/>
        </source>
    </evidence>
    <sequence length="571" mass="67220" checksum="63AEBC56D82C5855" modified="2020-10-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5Y7</accession>
    <name>A0A6I8W5Y7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclin-dependent kinase inhibitor 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238775.1">
        <property type="nucleotide sequence ID" value="XM_033382884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5Y7"/>
    <dbReference type="KEGG" id="dpo:6900014"/>
    <dbReference type="InParanoid" id="A0A6I8W5Y7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026096178" evidence="1">
        <location>
            <begin position="18"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238775.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="19637" checksum="46706618F124B0C5" modified="2020-08-12" version="1">MFAKIMLVALCVATAQAGLLPFHAGPVPLAAPVATAGVVAPYASSFNAHRINHAVAYPVAPAPALAPAPVAFAAPAPVPVAAPAPVAFAAPAPAFAAPAFAGFAPGFAAPAPLAFAAPNKVAFGLPAFAAPALAPAPPKVAFAAPAPAPALAPAPAPLAAPVAAPLGFAAPAPVAFAAPAKFGFGPFAAAPLAVGPKFAAPSPYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7D6</accession>
    <accession>Q29GJ5</accession>
    <name>A0A6I8W7D6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peroxisomal multifunctional enzyme type 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">Mfe2</name>
        <name evidence="12" type="synonym">LOC4815128</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <strain evidence="12">MV2-25</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355058.2">
        <property type="nucleotide sequence ID" value="XM_001355022.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239281.1">
        <property type="nucleotide sequence ID" value="XM_033383390.1"/>
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    <dbReference type="GeneID" id="4815128"/>
    <dbReference type="KEGG" id="dpo:4815128"/>
    <dbReference type="OMA" id="WATKVHT"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
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    <dbReference type="Bgee" id="FBgn0077449">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03448">
        <property type="entry name" value="HDE_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000185">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000061">
        <property type="entry name" value="Probable dehydrogenase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
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    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
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    <dbReference type="InterPro" id="IPR054357">
        <property type="entry name" value="MFE-2_N"/>
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    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
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    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
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        <property type="entry name" value="SDR_fam"/>
    </dbReference>
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        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22622">
        <property type="entry name" value="MFE-2_hydrat-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
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    <keyword evidence="8" id="KW-0456">Lyase</keyword>
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    <feature type="domain" description="Ketoreductase" evidence="10">
        <location>
            <begin position="12"/>
            <end position="186"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073497"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355058.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033239281.1"/>
        </source>
    </evidence>
    <sequence length="597" mass="63697" checksum="B6153068F0733441" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7E3</accession>
    <accession>Q29GJ2</accession>
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        <submittedName>
            <fullName evidence="8 9">HIG1 domain family member 2A, mitochondrial</fullName>
        </submittedName>
    </protein>
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        <name evidence="8 9" type="primary">LOC4815329</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="8 9">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355061.2">
        <property type="nucleotide sequence ID" value="XM_001355025.4"/>
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        <property type="nucleotide sequence ID" value="XM_033383392.1"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <location>
            <begin position="45"/>
            <end position="64"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001355061.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033239283.1"/>
        </source>
    </evidence>
    <sequence length="104" mass="11624" checksum="37E0029023285ECD" modified="2020-08-12" version="1">MSSKAQITLPEEELDWIQLRQDLGPVIEVETTKEKLQRKIKENPLVPIGCLATTAALTMGLYNFRTGNRKMSQLMMRTRIAAQGFTVAALIIGVVMTYGEKKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9C2</accession>
    <name>A0A6I8W9C2_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Peritrophin-48</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC26534294</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="CHIT_BIND_II"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026070394" evidence="1">
        <location>
            <begin position="23"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="35"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="119"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="2">
        <location>
            <begin position="195"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239988.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28137" checksum="F021C34E6C4B38B4" modified="2020-08-12" version="1">MKSVRVNLGLLLLFICTVQGQAQKQDYPPCDSDSFCICRDHQVGDLLPDCDDCSGYIYCGLDVIQKLKCSQGEIFDSRLSACVPGTCPRRDCTNSVLFPMHPAKSPKCFNKDGVNLDIFCICNDHQVGALLPNCDDCSGYIYCGLDVVQKMNCSKGEIFDSSLSACVPGTCPRRDCTNTGNPGDPVTPAGCTNKEVNCSFHGQILPHGQHCRLFWTCVEDCPMLGFCELGMWFDREKFVCDYPYNVRNCPINQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9G4</accession>
    <name>A0A6I8W9G4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Serine-rich adhesin for platelets isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6" type="primary">tay</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4 5">MV-25-SWS-2005</strain>
            <tissue evidence="3 4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239916.1">
        <property type="nucleotide sequence ID" value="XM_033384025.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239917.1">
        <property type="nucleotide sequence ID" value="XM_033384026.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239918.1">
        <property type="nucleotide sequence ID" value="XM_033384027.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239919.1">
        <property type="nucleotide sequence ID" value="XM_033384028.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899742"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249190">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W9G4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052824">
        <property type="entry name" value="m6A_RNA_Methylation_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13585">
        <property type="entry name" value="CHASCON, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13585:SF19">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="750"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1194"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1413"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1989"/>
            <end position="2091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2264"/>
            <end position="2313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="327"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1197"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1435"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2009"/>
            <end position="2083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2292"/>
            <end position="2301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2302"/>
            <end position="2313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033239919.1"/>
        </source>
    </evidence>
    <sequence length="2393" mass="250119" checksum="8B4331A5C670761E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDY4</accession>
    <name>A0A6I8WDY4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Complex III assembly factor LYRM7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">LYR motif-containing protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6896751</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Assembly factor required for Rieske Fe-S protein UQCRFS1 incorporation into the cytochrome b-c1 (CIII) complex. Functions as a chaperone, binding to this subunit within the mitochondrial matrix and stabilizing it prior to its translocation and insertion into the late CIII dimeric intermediate within the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with UQCRFS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241598.1">
        <property type="nucleotide sequence ID" value="XM_033385707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDY4"/>
    <dbReference type="FunCoup" id="A0A6I8WDY4">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6896751"/>
    <dbReference type="InParanoid" id="A0A6I8WDY4"/>
    <dbReference type="OMA" id="TRQYVFH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248224">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050435">
        <property type="entry name" value="MZM1/LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749:SF1">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="10">
        <location>
            <begin position="6"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="30"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033241598.1"/>
        </source>
    </evidence>
    <sequence length="137" mass="15596" checksum="5C594AED60576851" modified="2020-08-12" version="1">MSNLRRQALSAFKKLHRTRQYVFDGDVRALEEARQKINDEFRQNRVVTDQDDIQNKIKLAEECAQELRANVIQAHKREDGVYELRITPETTRMDNVVFNPDAIIESPRKRRGAKPAEGCCGGAAMAALEAEVAARKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEK4</accession>
    <name>A0A6I8WEK4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Retinol dehydrogenase 14 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241855.1">
        <property type="nucleotide sequence ID" value="XM_033385964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WEK4"/>
    <dbReference type="FunCoup" id="A0A6I8WEK4">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801451"/>
    <dbReference type="InParanoid" id="A0A6I8WEK4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000500">
        <property type="entry name" value="Blast:Retinol dehydrogenase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF66">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241855.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="37165" checksum="3B10727482FBA6AF" modified="2020-08-12" version="1">MGFVFGWLPTEHYVRDIFLLTLSAAIAATLMSIRFYLRITAGRCFTETKMEGKTVIITGANSGIGKETAKDLAGRGARIIMACRNLETANAVKDEIVKETNNNKIVVKKLDLGSQKSVREFAADIVKTESKIDVLIHNAGMALAFRGQTSEDGVELTMATNHYGPFLLTHLLIDLLKRSAPARIVIVASELYRLSSVNLNKLNPVGSFPAAYLYYVSKFANIYFARELAKRLEGTRVTVNFLHPGMIDSGIWRNVPFPLNLPMMAITKGFFKTTKAGAQTTIYLATSDEVANVNGKYFMDCKEATLNAAALDEEKGRKIWEESLKIAKITPQDPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-09-05" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I5AMM9</accession>
    <accession>A0A6I8V553</accession>
    <name>I5AMM9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c oxidase assembly protein COX11, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Cox11</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with CNNM4/ACDP4. Interacts with RANBP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_003736141.1">
        <property type="nucleotide sequence ID" value="XM_003736093.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I5AMM9"/>
    <dbReference type="FunCoup" id="I5AMM9">
        <property type="interactions" value="1279"/>
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    <dbReference type="KEGG" id="dpo:4818106"/>
    <dbReference type="eggNOG" id="KOG2540">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045000_1_3_1"/>
    <dbReference type="InParanoid" id="I5AMM9"/>
    <dbReference type="OMA" id="NKLECFC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076379">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.370.10:FF:000001">
        <property type="entry name" value="COX11 cytochrome c oxidase assembly homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
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    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
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    <dbReference type="NCBIfam" id="NF003465">
        <property type="entry name" value="PRK05089.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
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    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_003736141.1"/>
        </source>
    </evidence>
    <sequence length="245" mass="27737" checksum="D8D6691290E33286" modified="2012-09-05" version="1">MLRTLFAVRGQCQQLLRRTFTPHCSGQRPQQLLQCRIRMKSTDSPGEAARKLRLKSTLYYITAGGVLIMGLSYAAVPLYSIFCQAYSYGGTTSQGHDAEKVEHMVKVNDRVLKIRFNADIGSSMRWNFKPQQYEIKVAPGETALAFYTARNPTDKAVIGISTYNVIPFEAGAYFNKIQCFCFEEQQLNPHEEVDMPVFFYIDPEITQDPALEHCDTITLSYTFFEAKEGLKLNFPSYAKPHTAAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q293P8</accession>
    <name>CRY1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Cryptochrome-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cry</name>
        <name type="ORF">GA17677</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Blue light-dependent regulator that is the input of the circadian feedback loop. Has no photolyase activity for cyclobutane pyrimidine dimers or 6-4 photoproducts. Regulation of expression by light suggests a role in photoreception for locomotor activity rhythms. Light induces the degradation of cry, likely due to conformational change in the photoreceptor leading to targeting to the proteasome. Under circadian regulation, expression is influenced by the clock pacemaker genes period, timeless, Clock and cycle. Binding to tim irreversibly commits tim to proteasomal degradation. Functions, together with per, as a transcriptional repressor required for the oscillation of peripheral circadian clocks and for the correct specification of clock cells. Genes directly activated by the transcription factors Clock (Clk) and cycle (cyc) are repressed by cry. Necessary for light-dependent magnetosensitivity, an intact circadian system is not required for the magnetoreception mechanism to operate. Required for both the naive and trained responses to magnetic field, consistent with the notion that Cry is in the input pathway of magnetic sensing.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="2">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with tim and per; promoted by light conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <text evidence="2">Nuclear translocation initiates after the perception of a light signal. Accumulates in the perinuclear region about one hour before translocation into the nucleus. Translocation occurs through interaction with other Clock proteins such as tim and per (By similarity).</text>
    </comment>
    <comment type="domain">
        <text evidence="2">FAD-binding region regulates cry stability, cry-tim interaction, and circadian photosensitivity.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Photolyase/cryptochrome alpha/beta domain is sufficient for light detection and phototransduction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL29166.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001360014.2">
        <property type="nucleotide sequence ID" value="XM_001359977.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q293P8"/>
    <dbReference type="SMR" id="Q293P8"/>
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        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q293P8"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0286496">
        <property type="protein sequence ID" value="FBpp0284934"/>
        <property type="gene ID" value="FBgn0077687"/>
    </dbReference>
    <dbReference type="GeneID" id="4803241"/>
    <dbReference type="KEGG" id="dpo:4803241"/>
    <dbReference type="CTD" id="42305"/>
    <dbReference type="eggNOG" id="KOG0133">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010348_3_4_1"/>
    <dbReference type="InParanoid" id="Q293P8"/>
    <dbReference type="OMA" id="IWFRHGL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077687">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009882">
        <property type="term" value="F:blue light photoreceptor activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.579.10:FF:000004">
        <property type="entry name" value="Cryptochrome-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000243">
        <property type="entry name" value="Cryptochrome-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF17">
        <property type="entry name" value="CRYPTOCHROME-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0090">Biological rhythms</keyword>
    <keyword id="KW-0157">Chromophore</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-0600">Photoreceptor protein</keyword>
    <keyword id="KW-0675">Receptor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0716">Sensory transduction</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000348598" description="Cryptochrome-1">
        <location>
            <begin position="1"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta">
        <location>
            <begin position="5"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="410"/>
            <end position="412"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O77059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <sequence length="540" mass="62337" checksum="122BC57A039A1435" modified="2008-09-02" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-03-02" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q296Q5</accession>
    <name>SPNE_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Probable ATP-dependent RNA helicase spindle-E</fullName>
            <ecNumber>3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Homeless</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">spn-E</name>
        <name type="synonym">hls</name>
        <name type="ORF">GA16329</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable ATP-binding RNA helicase which plays a central role during spermatogenesis and oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi and govern the methylation and subsequent repression of transposons. Involved in the repression of LTR retrotransposon copia. Also involved in telomere regulation by repressing specialized telomeric retroelements HeT-A, TAHRE, and TART; Drosophila telomeres being maintained by transposition of specialized telomeric retroelements. Involved in telomeric trans-silencing, a repression mechanism by which a transposon or a transgene inserted in subtelomeric heterochromatin has the capacity to repress in trans in the female germline, a homologous transposon, or transgene located in euchromatin. Involved in the repression of testis-expressed Stellate genes by the homologous Su(Ste) repeats. Required for anteroposterior and dorsoventral axis formation during oogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Component of the nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13"/>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL28403.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q296Q5"/>
    <dbReference type="SMR" id="Q296Q5"/>
    <dbReference type="FunCoup" id="Q296Q5">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q296Q5"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0285897">
        <property type="protein sequence ID" value="FBpp0284335"/>
        <property type="gene ID" value="FBgn0076345"/>
    </dbReference>
    <dbReference type="GeneID" id="4802318"/>
    <dbReference type="KEGG" id="dpo:4802318"/>
    <dbReference type="CTD" id="41919"/>
    <dbReference type="eggNOG" id="KOG0920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002601_1_0_1"/>
    <dbReference type="InParanoid" id="Q296Q5"/>
    <dbReference type="OMA" id="QRSAYCS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076345">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q296Q5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001676">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase DExH7 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF113">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0221">Differentiation</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0469">Meiosis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0896">Oogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword id="KW-0744">Spermatogenesis</keyword>
    <feature type="chain" id="PRO_0000391919" description="Probable ATP-dependent RNA helicase spindle-E">
        <location>
            <begin position="1"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="126"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="355"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="935"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box">
        <location>
            <begin position="240"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="139"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="1433" mass="164469" checksum="B29FBEF73448C369" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FJ6</accession>
    <accession>A0A6I8UED8</accession>
    <name>Q29FJ6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">E2 NEDD8-conjugating enzyme</fullName>
            <ecNumber evidence="7">2.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NEDD8 carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">UbcE2M</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.34" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001354206.1">
        <property type="nucleotide sequence ID" value="XM_001354170.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FJ6"/>
    <dbReference type="SMR" id="Q29FJ6"/>
    <dbReference type="FunCoup" id="Q29FJ6">
        <property type="interactions" value="2426"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FJ6"/>
    <dbReference type="GeneID" id="4814171"/>
    <dbReference type="KEGG" id="dpo:4814171"/>
    <dbReference type="eggNOG" id="KOG0420">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_6_0_1"/>
    <dbReference type="InParanoid" id="Q29FJ6"/>
    <dbReference type="OMA" id="CQVDFPD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080300">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FJ6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061654">
        <property type="term" value="F:NEDD8 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000239">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="28"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001354206.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20910" checksum="D15EDDE6288A801A" modified="2006-04-04" version="1">MIKLFTLKQQKKDGEQKGSGGQQKKASAAQLRIQKDISEMNLPKTCVTEFPDADDLLNFKLIISPDEGFYRDGRFVFNFRVGSNYPHEPPKVKCATQVYHPNIDLEGNVCLNILREDWNPVLTINSIVYGLQFLFLEPNPEDPLNKEAADVLQSNRRQFEYNVKKAMRGGCVGETYFECCLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FT1</accession>
    <accession>A0A6I8VIV7</accession>
    <name>Q29FT1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integral membrane protein DGCR2/IDD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4814258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042295.1">
        <property type="nucleotide sequence ID" value="XM_015186809.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042297.1">
        <property type="nucleotide sequence ID" value="XM_015186811.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FT1"/>
    <dbReference type="STRING" id="46245.Q29FT1"/>
    <dbReference type="GeneID" id="4814258"/>
    <dbReference type="KEGG" id="dpo:4814258"/>
    <dbReference type="eggNOG" id="ENOG502S3KB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104784_0_0_1"/>
    <dbReference type="OMA" id="MTAASTW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077054">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FT1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042378">
        <property type="entry name" value="IDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15256">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN DGCR2/IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15256:SF6">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN DGCR2_IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015042295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015042297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="26942" checksum="81166F8D0C189EF3" modified="2008-10-14" version="2">MTAASTWLRPLGQAETEAETEPAAAPASSVLAAEATPIKTTRATRIAPTITKAATREKSSRTINGLDTTRRSRQRGCTQWTVVGAGIGVSLCLGERTMPTVTSLWLLPSNATSWLLLALLCLLGCLCPFTDALEEDCVDFQGNKVNHGMLYVPGPGVCSLCVCYHSEPLWCKAIYCDPPYFCKNFRVGERCCEFECLDPPGEDKLYQERMRKRAEILAGNSTATNVRLAPLAMSSIMLGFLGNSLLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FV2</accession>
    <accession>A0A6I8UEU6</accession>
    <name>Q29FV2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microsomal glutathione S-transferase 1</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Mgstl</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16438"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotrimer; The trimer binds only one molecule of glutathione.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAPEG family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001354424.3">
        <property type="nucleotide sequence ID" value="XM_001354388.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FV2"/>
    <dbReference type="SMR" id="Q29FV2"/>
    <dbReference type="FunCoup" id="Q29FV2">
        <property type="interactions" value="553"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FV2"/>
    <dbReference type="GeneID" id="4814313"/>
    <dbReference type="KEGG" id="dpo:4814313"/>
    <dbReference type="eggNOG" id="ENOG502S0BD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105467_0_0_1"/>
    <dbReference type="InParanoid" id="Q29FV2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074534">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FV2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.550:FF:000002">
        <property type="entry name" value="Microsomal glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040162">
        <property type="entry name" value="MGST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689:SF6">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001354424.3"/>
        </source>
    </evidence>
    <sequence length="152" mass="16923" checksum="8330CD7C4D839004" modified="2016-01-20" version="3">MAGVVELLSLNNPVFKSYVFWAGVLVIKMLLMSLLTAVQRFKTKTFANPEDLMSPKLKVKFDDPNVERVRRAHRNDLENIVPFFIIGLLYTLTNPSVFLAVNLFRAVGIARIVHTIVYAVVVVPQPSRALAFFVALGATVYMALQVILAAAF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-01" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29HZ1</accession>
    <name>LANC3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>LanC-like protein 3 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA15215</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LanC-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EAL31616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29HZ1"/>
    <dbReference type="SMR" id="Q29HZ1"/>
    <dbReference type="FunCoup" id="Q29HZ1">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0287199">
        <property type="protein sequence ID" value="FBpp0285637"/>
        <property type="gene ID" value="FBgn0075239"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0371540">
        <property type="protein sequence ID" value="FBpp0333708"/>
        <property type="gene ID" value="FBgn0075239"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814476"/>
    <dbReference type="eggNOG" id="KOG2787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036244_0_1_1"/>
    <dbReference type="InParanoid" id="Q29HZ1"/>
    <dbReference type="OMA" id="GTSAIMH"/>
    <dbReference type="PhylomeDB" id="Q29HZ1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075239">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29HZ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031179">
        <property type="term" value="P:peptide modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04794">
        <property type="entry name" value="euk_LANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000012">
        <property type="entry name" value="LanC-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007822">
        <property type="entry name" value="LANC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020464">
        <property type="entry name" value="LanC-like_prot_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736">
        <property type="entry name" value="LANC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736:SF7">
        <property type="entry name" value="LANC-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05147">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01951">
        <property type="entry name" value="LANCEUKARYTE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01950">
        <property type="entry name" value="LANCSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01260">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158745">
        <property type="entry name" value="LanC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000285254" description="LanC-like protein 3 homolog">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="420" mass="47580" checksum="316D4C674A943D26" modified="2006-04-04" version="1">MERRYLKNPFQDFIGGENTAFASDEEHIKNLICTYVDTILEHCHPNSDDEDDRGDLYVGNAGIAFMFWKLASCEQTRDLYPPALQHASAFIRNAKANAERFKKRSAERYSFLCGNAGIYAVSAAISQAVKDTEELSNDLANFKSGIPSSKEFIHTKNGCDEVLVGRAGYLSGCYWLNDVLPDKKITDDDLISICQLIVTSGREYSKMNNSPLPLMFQYHGTEYLGAAHGLCAILHMLLDSPWFRTVPISAPAAELREIKRSIDYFLVLQDAEGNFPVALEDLRSGRDKRLVHWCHGAPGAVYMLAKAYLIFKEEKYLTSLRRSADLVWKKGFLRKGPGICHGVAGNGYVFLLLFRLTNEMKYLYRAHKFMELLTNSEFKQRARIPDNPHSLYEGVAGTVCFLVDILEPEQAYFPFMDVFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q29K65</accession>
    <accession>A0A6I8UJ07</accession>
    <name>Q29K65_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">p38b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001356250.2">
        <property type="nucleotide sequence ID" value="XM_001356214.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29K65"/>
    <dbReference type="SMR" id="Q29K65"/>
    <dbReference type="FunCoup" id="Q29K65">
        <property type="interactions" value="1635"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29K65"/>
    <dbReference type="GeneID" id="4816406"/>
    <dbReference type="KEGG" id="dpo:4816406"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="Q29K65"/>
    <dbReference type="OMA" id="NRYTDLN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080315">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29K65">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071243">
        <property type="term" value="P:cellular response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071276">
        <property type="term" value="P:cellular response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034614">
        <property type="term" value="P:cellular response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008340">
        <property type="term" value="P:determination of adult lifespan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038001">
        <property type="term" value="P:paracrine signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045793">
        <property type="term" value="P:positive regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048082">
        <property type="term" value="P:regulation of adult chitin-containing cuticle pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900407">
        <property type="term" value="P:regulation of cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07851">
        <property type="entry name" value="STKc_p38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000063">
        <property type="entry name" value="Mitogen-activated protein kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000769">
        <property type="entry name" value="Mitogen-activated protein kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008352">
        <property type="entry name" value="MAPK_HOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01773">
        <property type="entry name" value="P38MAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356250.2"/>
        </source>
    </evidence>
    <sequence length="365" mass="42265" checksum="23416B6AA609CED9" modified="2008-10-14" version="2">MSRKMPKFYKLDINRTEWEIPETYQSLQPVGQGAYGQVCKAVVRGTSTKVAIKKLARPFQSAVHAKRTYRELRLLKHMEHENVIGLLDVFHPGQPADSLDQFQQVYMVTHLMDADLNNIIRTQKLSDDHVQFLIYQILRGLKYIHSAGVIHRDLKPSNIAVNEDCELRILDFGLARPAESEMTGYVATRWYRAPEIMLNWMHYNKTVDIWSVGCIMAELLTGRTLFPGTDHIHQLNLIMEVLGTPAEEFMSRISSESARNYIRSLPVMPRRNFRDIFRGANPLAIDLLEKMLELDADQRITAEQALAHPYMEKYHDPTDEQTAALYDQSFEENELPVEKWREMVFTEVQAFRPTQAFAELLPKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29K77</accession>
    <accession>A0A6I8UJ77</accession>
    <name>Q29K77_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA polymerase subunit gamma-2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PolG2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356238.3">
        <property type="nucleotide sequence ID" value="XM_001356202.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29K77"/>
    <dbReference type="SMR" id="Q29K77"/>
    <dbReference type="FunCoup" id="Q29K77">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29K77"/>
    <dbReference type="GeneID" id="4816345"/>
    <dbReference type="KEGG" id="dpo:4816345"/>
    <dbReference type="eggNOG" id="KOG2298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067408_0_0_1"/>
    <dbReference type="InParanoid" id="Q29K77"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077280">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02426">
        <property type="entry name" value="Pol_gamma_b_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000040">
        <property type="entry name" value="DNA polymerase gamma 35kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042064">
        <property type="entry name" value="POLG2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF8">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Anticodon-binding" evidence="5">
        <location>
            <begin position="265"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001356238.3"/>
        </source>
    </evidence>
    <sequence length="362" mass="41079" checksum="A8FA5E3378B55436" modified="2016-01-20" version="3">MSRLSRCFRSLESAGFFRVLDDSHVELLSHGHAYADLLKQQWLSLRPLAAHLGTSHSTEAVSSTHLQRFSFSRTELFRSHFQQLVQEHPRKAKCPTLLKHLCTSYSCQPNPFLRHKAPVTHLITDFLVEPYRALEHFYNMQRESKIWWMRYSSNPSRYRIVPHELPEEADAKDCQGIDIISSYGKEGRVAVEQLSLVMLHGDDFRLPDARTDQSLPPAVIRSVIGLETATCALLLDGCDHGRETQSLLLNRMLAPYQCGIACLEVDPAQAGDLRDLCLHIKDVLQRAGLRLCQGVGYALSKDASQLTEHVDKSDMLGVPYTLLLSEQTLQNGLLQLRSRDTRLAETIHISDVPDYLLNIFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LJ5</accession>
    <accession>A0A6I8UKN4</accession>
    <name>Q29LJ5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Dim1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes that are involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).</text>
    </comment>
    <comment type="function">
        <text evidence="8">Plays role in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U5 snRNP complex. Component of the U4/U6-U5 tri-snRNP complex. The U4/U6-U5 tri-snRNP complex is a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. Directly interacts with CD2BP2. Interacts with HNRPF, HNRPH2, NEDD9 and PQBP1. Interacts with ERBB4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DIM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356984.1">
        <property type="nucleotide sequence ID" value="XM_001356948.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LJ5"/>
    <dbReference type="SMR" id="Q29LJ5"/>
    <dbReference type="FunCoup" id="Q29LJ5">
        <property type="interactions" value="1614"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LJ5"/>
    <dbReference type="GeneID" id="4817580"/>
    <dbReference type="KEGG" id="dpo:4817580"/>
    <dbReference type="eggNOG" id="KOG3414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117348_0_0_1"/>
    <dbReference type="InParanoid" id="Q29LJ5"/>
    <dbReference type="OMA" id="GMYELYD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075912">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LJ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000004">
        <property type="entry name" value="Spliceosomal protein DIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356984.1"/>
        </source>
    </evidence>
    <sequence length="142" mass="16782" checksum="F77D083A9F5AC7A6" modified="2006-04-04" version="1">MSYMLPHLHNGWQVDQAILSEEDRVVVIRFGHDWDPACMKMDEVMYSIAEKVKNFAVIYLVDITEVPDFNKMYELYDPCTVMFFFRNKHIMIDLGTGNNNKINWPLEDKQEMIDIVETVYRGARKGRGLVVSPKDYSTKYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LQ3</accession>
    <accession>A0A6I8UKQ4</accession>
    <name>Q29LQ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Exosome complex component RRP40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ribosomal RNA-processing protein 40</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Rrp40</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRP40 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356926.1">
        <property type="nucleotide sequence ID" value="XM_001356890.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LQ3"/>
    <dbReference type="FunCoup" id="Q29LQ3">
        <property type="interactions" value="1328"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LQ3"/>
    <dbReference type="GeneID" id="4817508"/>
    <dbReference type="KEGG" id="dpo:4817508"/>
    <dbReference type="eggNOG" id="KOG1004">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069847_5_1_1"/>
    <dbReference type="InParanoid" id="Q29LQ3"/>
    <dbReference type="OMA" id="SYMAFPN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076595">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22526">
        <property type="entry name" value="KH-I_Rrp40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05790">
        <property type="entry name" value="S1_Rrp40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000112">
        <property type="entry name" value="Exosome complex component RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000038">
        <property type="entry name" value="exosome complex component RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049469">
        <property type="entry name" value="RRP40_KH-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041054">
        <property type="entry name" value="Rrp40_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037319">
        <property type="entry name" value="Rrp40_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18311">
        <property type="entry name" value="Rrp40_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21262">
        <property type="entry name" value="RRP40_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exosome complex exonuclease Rrp40 N-terminal" evidence="12">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="151"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001356926.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="25391" checksum="C7C9DACD8402467B" modified="2006-04-04" version="1">MDCSNAIVMPGERITIIEEVAKEKRVILGPGLRRLDDTVVASKAGPLRHKEPGTYWIDNYQRRYVPARGDLVLGIIKGKAGDQYRVDIGSADIASISYLAFEAATKKNRPDLNPGDIIYARVLNASADIEPELVCVNSNGKRGKLGLLADGFFFKCTLNLGRLLLRENCPALAALTRELPYEIAIGVNGRIWVKANSLRETMALSNAILTMEQAGYTEIDKICDSLGDVLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q29N03</accession>
    <accession>A0A6I8UJJ0</accession>
    <name>Q29N03_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Herp</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356476.3">
        <property type="nucleotide sequence ID" value="XM_001356440.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29N03"/>
    <dbReference type="SMR" id="Q29N03"/>
    <dbReference type="FunCoup" id="Q29N03">
        <property type="interactions" value="978"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29N03"/>
    <dbReference type="GeneID" id="4817184"/>
    <dbReference type="KEGG" id="dpo:4817184"/>
    <dbReference type="eggNOG" id="KOG4583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033843_0_0_1"/>
    <dbReference type="InParanoid" id="Q29N03"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073099">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29N03">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01790">
        <property type="entry name" value="Ubl_HERP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000046">
        <property type="entry name" value="Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039751">
        <property type="entry name" value="HERPUD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943:SF27">
        <property type="entry name" value="HOMOCYSTEINE-INDUCED ENDOPLASMIC RETICULUM PROTEIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UNIQUITIN-LIKE DOMAIN HERPUD PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356476.3"/>
        </source>
    </evidence>
    <sequence length="492" mass="53186" checksum="396FF244320A1C4D" modified="2016-01-20" version="3">MDDTAASPAAATAAGCDAARDKTNAGVAVAAAAAASSTSVRLLIKSSNQQYDDMNVDSDLSWTVQRLKKQLSLVYPGKPDINDQKLIYSGKLLDDAQKLSEVIRSYKDVYQQHHIFHLVCANKHFSKTPAMPTAVPKNAAPRQEGNLNASTNEIRQRHATNQQQQQPPLQLPQQQAGVDSFLHGDVNNLAFRTWYPLWLQQQQAATHPDPHVAYQQRVLLYNAWMSQVYDAYLQDLMRGAESPPGNRVPLLPNMMPQWELQAQQQMAAHAAASAAAAQQNPAPVAQPAEAAQPAANRPNFPLIQEEPENRDWLDSFFSFTRLAIFVTVLYFNSSPLRCLLVMFIAGVIYLYHIGVLRRRRERNNNNINRNNNAGHPVAAFAAVQQIQRMMDAVERENNDPQAAVEPVAAGQDAPDAPLGAAADAPADPAAAPAAAAPAAPVPASAAGPEQAAAVSAEAAAVEPPNANNSVISAVRTFVITFFTSLLPEAPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NY27</accession>
    <accession>A0A6I8V9L1</accession>
    <name>A0A0R3NY27_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26531996</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036083.1">
        <property type="nucleotide sequence ID" value="XM_015180597.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NY27"/>
    <dbReference type="GeneID" id="26531996"/>
    <dbReference type="KEGG" id="dpo:26531996"/>
    <dbReference type="InParanoid" id="A0A0R3NY27"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0270983">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015036083.1"/>
        </source>
    </evidence>
    <sequence length="48" mass="5150" checksum="E22D46D52F821815" modified="2016-01-20" version="1">MKTPDTKAKLLNNISLSKHLSSKKGGSRTSNCGNCLEFSVLTRLSSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NZY7</accession>
    <accession>A0A6I8V970</accession>
    <name>A0A0R3NZY7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DnaJ protein homolog 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036377.1">
        <property type="nucleotide sequence ID" value="XM_015180891.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NZY7"/>
    <dbReference type="SMR" id="A0A0R3NZY7"/>
    <dbReference type="FunCoup" id="A0A0R3NZY7">
        <property type="interactions" value="510"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3NZY7"/>
    <dbReference type="GeneID" id="4817618"/>
    <dbReference type="InParanoid" id="A0A0R3NZY7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078585">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NZY7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000033">
        <property type="entry name" value="dnaJ homolog subfamily B member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000026">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF553">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015036377.1"/>
        </source>
    </evidence>
    <sequence length="350" mass="39619" checksum="BAE0F71EE1957986" modified="2016-01-20" version="1">MGKDYYKTLGLPKTATDEEIKKAYRKLALRYHPDKNKAANAEEKFKEVAEAYEVLSDKSKREVYDKYGEDGLKSGGTRNGGNTNKTFTYQFHGDPRATFAQFFGNSNPFAPFFDMGDNLFDKNVFDLDTEPDFFSSPFGGIGSRHGLGSAFRPSFRSHSFNVHTPFKKEQKQDPPVEHDLYVTLEEIYHGCVKKMKISRRVVQADGSSRKEDKVLQISIKPGWKSGTKVTFQKEGDQAPGKIPADIVFIIRDKPHAMFKREGSDLRYTARLTLKQALCGVVFQVPTMSGDKLRISTMQEIIKPNTVKRIQGYGLPFPKDTTRKGDLLVAFDIQFPEKLTAAQKEVLKDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UC67</accession>
    <name>A0A6I8UC67_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acidic leucine-rich nuclear phosphoprotein 32 family member B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EAChm</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353181.3">
        <property type="nucleotide sequence ID" value="XM_001353145.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UC67"/>
    <dbReference type="KEGG" id="dpo:4812961"/>
    <dbReference type="InParanoid" id="A0A6I8UC67"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026123204" evidence="2">
        <location>
            <begin position="21"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353181.3"/>
        </source>
    </evidence>
    <sequence length="206" mass="22652" checksum="39C7B9DCFBDD2B21" modified="2020-08-12" version="1">MRSLALAVVIFAGILAYSSAAPQITRGDLTWLENILRGLGSQNSTIRFGNNTLIVSGVSTASANVNPSPAEPHRPHHPNQSWGNWGPGYGGYWRMVPSENGEIVEELENNNSYDSNDFAFDLAEDESVEDIIEEDQSDEDQSISDASSEAEDELDEEADEDEDDLEEGSDQEDVNESQDEEVDPEEDAADKELDEIESELPAEKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCN2</accession>
    <name>A0A6I8UCN2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="16">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SdhAL</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="14">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="16">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Mitochondrion inner membrane</location>
            <topology evidence="1 16">Peripheral membrane protein</topology>
            <orientation evidence="1 16">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="16"/>
    <dbReference type="RefSeq" id="XP_001353277.4">
        <property type="nucleotide sequence ID" value="XM_001353241.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCN2"/>
    <dbReference type="FunCoup" id="A0A6I8UCN2">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813894"/>
    <dbReference type="InParanoid" id="A0A6I8UCN2"/>
    <dbReference type="OMA" id="CHYFVDY"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079079">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCN2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000004">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 14 16" id="KW-0274">FAD</keyword>
    <keyword evidence="4 14 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <keyword evidence="16" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="17">
        <location>
            <begin position="45"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="18">
        <location>
            <begin position="497"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="50"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="441"/>
            <end position="442"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="15">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001353277.4"/>
        </source>
    </evidence>
    <sequence length="648" mass="71493" checksum="ABBBF72C2BB059F9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UD14</accession>
    <name>A0A6I8UD14_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4812887</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the transition zone in primary cilia. Required for ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM237 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353531.4">
        <property type="nucleotide sequence ID" value="XM_001353495.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UD14"/>
    <dbReference type="FunCoup" id="A0A6I8UD14">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812887"/>
    <dbReference type="InParanoid" id="A0A6I8UD14"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029409">
        <property type="entry name" value="TMEM237"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15383">
        <property type="entry name" value="TMEM237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="504"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="613"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="650"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="210"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="266"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="578"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001353531.4"/>
        </source>
    </evidence>
    <sequence length="742" mass="78862" checksum="7D42371300420D90" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDI9</accession>
    <accession>Q2LZE2</accession>
    <name>A0A6I8UDI9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Larval cuticle protein 65Ag1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">LOC4813481</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353829.1">
        <property type="nucleotide sequence ID" value="XM_001353793.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239053.1">
        <property type="nucleotide sequence ID" value="XM_033383162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDI9"/>
    <dbReference type="GeneID" id="4813481"/>
    <dbReference type="KEGG" id="dpo:4813481"/>
    <dbReference type="OMA" id="GFQPQEH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031305">
        <property type="entry name" value="Casein_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF218">
        <property type="entry name" value="ADULT CUTICLE PROTEIN 65AA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00306">
        <property type="entry name" value="CASEIN_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0193">Cuticle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634429" evidence="4">
        <location>
            <begin position="19"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001353829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033239053.1"/>
        </source>
    </evidence>
    <sequence length="108" mass="11756" checksum="8175E0DD8A01DDB2" modified="2020-08-12" version="1">MKFTIAFSVACLLATALAAPPASQQEAQVLRFDSDVQPEGYNFAVETSDGKRHQEEGELKDVGTDHEALVVRGSYSYVGDDGQTYAITYLADKYGFQPEGAHLPRAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDJ5</accession>
    <name>A0A6I8UDJ5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CAAX prenyl protease 2</fullName>
            <ecNumber evidence="11">3.4.26.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Farnesylated proteins-converting enzyme 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Sras</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001353747.4">
        <property type="nucleotide sequence ID" value="XM_001353711.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDJ5"/>
    <dbReference type="FunCoup" id="A0A6I8UDJ5">
        <property type="interactions" value="1964"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813878"/>
    <dbReference type="InParanoid" id="A0A6I8UDJ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDJ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 16" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="14">
        <location>
            <begin position="151"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001353747.4"/>
        </source>
    </evidence>
    <sequence length="306" mass="34756" checksum="4C6864A238400E70" modified="2020-08-12" version="1">MINASEMVAKATVQPVLEVLPSQDPLPQIPIITSVTCCFVLSLLYVGSLYVWNTKHNRDHPATIKRRFTSVSVVMIFAPFFVWYFSSPELLSSEPFAKLLGFRWEGLWQAVVIPYGLTMLLFAGPIFVNCQNESIGSYFDLDYWRGCIHNIMWVRNHVMAPLSEEFVFRACMMPLILQSFSPMTAVFITPLFFGVAHLHHIAERLSLGVELSTALLIGLFQFIYTTLFGFYSAFLFARTGHIVAPFVVHAFCNHMGLPDLQDLWQQDMWRRVLAIVLYVAGLAGWILLLPIATSPSVYGNTLYWNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDQ7</accession>
    <name>A0A6I8UDQ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Probable insulin-like peptide 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Ilp1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterodimer of a B chain and an A chain linked by two disulfide bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the insulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353876.4">
        <property type="nucleotide sequence ID" value="XM_001353840.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDQ7"/>
    <dbReference type="FunCoup" id="A0A6I8UDQ7">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813355"/>
    <dbReference type="InParanoid" id="A0A6I8UDQ7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDQ7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04366">
        <property type="entry name" value="IlGF_insulin_bombyxin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.100.10">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022352">
        <property type="entry name" value="Ins/IGF/rlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016179">
        <property type="entry name" value="Insulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036438">
        <property type="entry name" value="Insulin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13647:SF4">
        <property type="entry name" value="INSULIN-LIKE PEPTIDE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13647">
        <property type="entry name" value="INSULIN-LIKE PEPTIDE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00049">
        <property type="entry name" value="Insulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00276">
        <property type="entry name" value="INSULINFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00078">
        <property type="entry name" value="IlGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56994">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Insulin-like" evidence="6">
        <location>
            <begin position="54"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001353876.4"/>
        </source>
    </evidence>
    <sequence length="145" mass="16248" checksum="F5023D5151887F0E" modified="2020-08-12" version="1">MPSNSQNRLSHRMLGKDTSAITAVQVSRRHWPLVMLIFLAVVQGHVHQLQQGSHKLCGQALSDAMDVVCAHGYNTLPQKKSSTVDAQDDEVPEYALSPLLTSLYGAEVLIKTRRHRRQRSSGGIYDECCVYSCRYEELASYCLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDT6</accession>
    <name>A0A6I8UDT6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD repeat-containing protein 92</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Wdr92</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353964.2">
        <property type="nucleotide sequence ID" value="XM_001353928.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDT6"/>
    <dbReference type="FunCoup" id="A0A6I8UDT6">
        <property type="interactions" value="291"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813303"/>
    <dbReference type="InParanoid" id="A0A6I8UDT6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDT6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353964.2"/>
        </source>
    </evidence>
    <sequence length="375" mass="41143" checksum="D883FB62363418FE" modified="2020-08-12" version="1">MDKPQLIEHLNASVNYTVYDTKWIPLSAKFVVLGSKANSHGIMDIYELNEDQLVKVKTVEKKVAFKCGTFGASSLRNRHIAIGDFEGRLQVLDMERPDLPVYNVKAHTAIINTIDAIGGNQLDCGAPEIVTGSRDGAVKVWDIRQGQSPVVDISPPPQMGDGINQSSGRRDCWAVAFGNTFNSEERMVAAGYDNGDLKLFDLRTLSVRWEASMKNGICGLEFDRRDIPMNKLAVTTLEGGLLVYDMRHQHPTKGFSYVEERNAGRSVGTNGVISGPKATVWVARHLPQNRDVFLTGGGTGSIRLWQYEYPDKRVVVDADGTKTGVAGTLHMISAATLSSQPVHCFDWHPDKLGLAVCGAFDQSVRVLISTKLNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG66</accession>
    <name>A0A6I8UG66_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC4815345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354973.4">
        <property type="nucleotide sequence ID" value="XM_001354937.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041848.2">
        <property type="nucleotide sequence ID" value="XM_015186362.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UG66"/>
    <dbReference type="KEGG" id="dpo:4815345"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UG66">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354973.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015041848.2"/>
        </source>
    </evidence>
    <sequence length="141" mass="15419" checksum="EFBE1C2CEF637B45" modified="2020-08-12" version="1">MDMRMELESYVQSSPNTNRFVVEDSITKVTSDGEPHGMMTMVAGLSGLLAAIIILAVLASMVACRKQKSTTSNKSSPSSRRLSKKSMNLAMTIVEEEQPQLAGNLNAAFAESTLTLQGQEKDKEKDTQWSTTPTSVVVQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG89</accession>
    <name>A0A6I8UG89_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell wall protein DAN4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4815340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354999.3">
        <property type="nucleotide sequence ID" value="XM_001354963.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UG89"/>
    <dbReference type="FunCoup" id="A0A6I8UG89">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815340"/>
    <dbReference type="InParanoid" id="A0A6I8UG89"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UG89">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026291543" evidence="3">
        <location>
            <begin position="22"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001354999.3"/>
        </source>
    </evidence>
    <sequence length="318" mass="33501" checksum="118C4E9421F35EB3" modified="2020-08-12" version="1">MNVLPLLWLLCGSSLLLLASGEQNAAMASVAANPQPSQSNSSTAAPLVNVVNATEHTAVPGQVHIIEKTQVKSTNSTELAKEHEKDMNKTSTIPPDNTAKNRKRTDPVQLKPTTATAAPTSVASAANNATVTKPAAAAAAATVKPSTGTIVADGISINDVINANNSTTSTTSSSRSSSSTSTTPSTTTTTTTTTTTTTTTPRPTKPPVVYSMDTHSEWEKEQEGKVKSPAAPSLAASSEPLPVPASTLSEPVAPMVQELTGNLADREGNDYIVPIVTVLLTVPLAIGVVTIMYRRFRELWSTRHYRRMDFLVDGMYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGX1</accession>
    <name>A0A6I8UGX1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="9">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Dbct</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_001355312.2">
        <property type="nucleotide sequence ID" value="XM_001355276.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGX1"/>
    <dbReference type="FunCoup" id="A0A6I8UGX1">
        <property type="interactions" value="1950"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815955"/>
    <dbReference type="InParanoid" id="A0A6I8UGX1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGX1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031405">
        <property type="term" value="F:lipoic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000013">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000027">
        <property type="entry name" value="Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050743">
        <property type="entry name" value="2-oxoacid_DH_E2_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178:SF5">
        <property type="entry name" value="LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="11">
        <location>
            <begin position="38"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="12">
        <location>
            <begin position="159"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="117"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355312.2"/>
        </source>
    </evidence>
    <sequence length="462" mass="49917" checksum="14747CF386B651F5" modified="2020-08-12" version="1">MATHLLRNGASSWLLKNYLSRHQTLKRCLHVTPSLDKTVSFNLSDIGEGIREVTVKEWFVKVGDTVEQFDNLCEVQSDKASVTITSRYDGKITKIHHSIDEIALVGKPLLDFDVLDEEGDENSTESSSSSSDSSSSEAEAAKPSAGEAVSINGGRVITPATPAVRRLAKEHQLDLANVPPTGKNGRVLKGDILEYLGQVPPGTNIPHPTIAAKQQSQVARTTAGTAAGIPADRVEVLKGVRKAMLKAMTESLKIPHFAYSDEIDMSNLVQFRAQLQAMAKESGVPKLTFMPFCIKAASIALTKYPIVNSSLDLASESLVYKGAHNISVAIDTPQGLVVPNIKNCQSKNIIEIAKDLNALVERGRTGSLTPTDFADGTFSLSNIGIIGGTYTHPCIMAPQVAIGAMGRTKAVPRFNDKDEVVKAQIMSVSWSADHRVIDGVTMASFSNVWKQYLEQPALFLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHN8</accession>
    <name>A0A6I8UHN8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein Obp8a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Obp8a</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355631.3">
        <property type="nucleotide sequence ID" value="XM_001355595.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHN8"/>
    <dbReference type="FunCoup" id="A0A6I8UHN8">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815875"/>
    <dbReference type="InParanoid" id="A0A6I8UHN8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UHN8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23992">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.20">
        <property type="entry name" value="Pheromone/general odorant binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006170">
        <property type="entry name" value="PBP/GOBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036728">
        <property type="entry name" value="PBP_GOBP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01395">
        <property type="entry name" value="PBP_GOBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47565">
        <property type="entry name" value="Insect pheromone/odorant-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026344797" evidence="1">
        <location>
            <begin position="29"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355631.3"/>
        </source>
    </evidence>
    <sequence length="164" mass="18489" checksum="FE85C2E861C9E356" modified="2020-08-12" version="1">MGQRSLICLPLLLLLLGPLGVLQSRAMARPTPTPPLRAPQSLALLRARDQCSAHLSHAQRMRMDRMQYENLPHVQRYVHCFWSRLQLWHDGTGFDALGIVHSFGGPRRLNVEQALPAINGCNAKARRVSHGVSDWCYRAFACVLKTPVGDWYRRHMADVINGNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK95</accession>
    <name>A0A6I8UK95_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WW domain-containing oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Wwox</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356875.3">
        <property type="nucleotide sequence ID" value="XM_001356839.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK95"/>
    <dbReference type="FunCoup" id="A0A6I8UK95">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817102"/>
    <dbReference type="InParanoid" id="A0A6I8UK95"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000032">
        <property type="entry name" value="WW domain containing oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000353">
        <property type="entry name" value="WW domain-containing oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
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    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="WW" evidence="18">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="18">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_001356875.3"/>
        </source>
    </evidence>
    <sequence length="407" mass="46374" checksum="65783F47AA5AD273" modified="2020-08-12" version="1">MMSLPDTDSEDELPPGWEERATDDGTVCYVNQQNKTSQWTHPRTGRSKRITGELPIGWEKYYDEQLKKFIFLNKETQQRSNVDPRLAFAVEEPTQNVAQVRQRFDSCSTALQVLHGKDLHGRIALITGANCGIGFETARSLTQHGCEIIFACRSRASAEAAIDRIAQERPSARARCRFVALDLSSLRAVRQFVREIKESVSHIDYLILNAGVFALPYSTTVDGLETTFQVSHLSHFYLTMQLETLFDYKTRIVVLSSESHRFANLPVDNLAIHHVSPPAEKYWSMMAYNNAKLCNVLFAQELAQRWKQRGISVFSVHPGNMVSTELSRNYWFYRWLFAIVRPFTKSLQQAAATSIYCATANELTGLSGLYFNNCYFCEPSKLSKSAALQQQLWHLSEHLVSELLEDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKA7</accession>
    <name>A0A6I8UKA7_DROPS</name>
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        <submittedName>
            <fullName evidence="11">Protein brunelleschi</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">brun</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIBP family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001356833.4"/>
    </dbReference>
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        <property type="interactions" value="2109"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816931"/>
    <dbReference type="InParanoid" id="A0A6I8UKA7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058565">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_1st"/>
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        <property type="entry name" value="Ig_TRAPPC9_Trs120_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058568">
        <property type="entry name" value="Ig_TRAPPC9_Trs120_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058564">
        <property type="entry name" value="TPR_TRAPPC9_Trs120"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013935">
        <property type="entry name" value="Trs120_TRAPPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058563">
        <property type="entry name" value="Trs120_TRAPPC9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21512:SF5">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26254">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26282">
        <property type="entry name" value="Ig_TRAPPC9-Trs120_3rd"/>
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    </dbReference>
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        <property type="entry name" value="Ig_TRAPPC9-Trs120_4th"/>
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    </dbReference>
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        <property type="entry name" value="TPR_TRAPPC9-Trs120"/>
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    <dbReference type="Pfam" id="PF08626">
        <property type="entry name" value="TRAPPC9-Trs120"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trs120/TRAPPC9 N-terminal" evidence="5">
        <location>
            <begin position="227"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 TPR region" evidence="6">
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            <end position="612"/>
        </location>
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    <feature type="domain" description="Trs120/TRAPPC9 first Ig-like" evidence="7">
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            <begin position="655"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 third Ig-like" evidence="8">
        <location>
            <begin position="1029"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="Trs120/TRAPPC9 fourth Ig-like" evidence="9">
        <location>
            <begin position="1153"/>
            <end position="1308"/>
        </location>
    </feature>
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        <location>
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            <end position="343"/>
        </location>
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        <location>
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        <location>
            <begin position="928"/>
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    </feature>
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        <location>
            <begin position="324"/>
            <end position="336"/>
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        <location>
            <begin position="365"/>
            <end position="375"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="383"/>
            <end position="405"/>
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            <begin position="945"/>
            <end position="958"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008459"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08626"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF26251"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF26254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001356869.2"/>
        </source>
    </evidence>
    <sequence length="1311" mass="145552" checksum="D945214E05DE809B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL51</accession>
    <name>A0A6I8UL51_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Breast cancer anti-estrogen resistance protein 3 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4817868</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357201.4">
        <property type="nucleotide sequence ID" value="XM_001357165.4"/>
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        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817868"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UL51">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10337">
        <property type="entry name" value="SH2_BCAR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000013">
        <property type="entry name" value="SH2 domain-containing protein 3C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000015">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
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        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="SH2-Ras-GEF_adapter"/>
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    <dbReference type="InterPro" id="IPR044102">
        <property type="entry name" value="SH2_SHEP1/BCAR3/NSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14247">
        <property type="entry name" value="BREAST CANCER ANTI-ESTROGEN RESISTANCE PROTEIN 3 HOMOLOG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14247:SF8">
        <property type="entry name" value="RAS-GEF DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASGEF_CAT"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="241"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="6">
        <location>
            <begin position="695"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="375"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="529"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="395"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="539"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001357201.4"/>
        </source>
    </evidence>
    <sequence length="980" mass="109278" checksum="BD16B3DF0FDADCA9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMR5</accession>
    <name>A0A6I8UMR5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peritrophin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4800750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357967.4">
        <property type="nucleotide sequence ID" value="XM_001357930.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMR5"/>
    <dbReference type="KEGG" id="dpo:4800750"/>
    <dbReference type="InParanoid" id="A0A6I8UMR5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026285890" description="Peritrophin-1" evidence="1">
        <location>
            <begin position="36"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357967.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21109" checksum="A6A34856D103A282" modified="2020-08-12" version="1">MFCMPFVMKLFMKNKWNTLLLMVVLLVLLPPLGAARDTSDPDTHTMNATCKHATDMWGDPNPNLFYVCSADGQQPLQLQCPQGRGFFNGLGFLGCLPYDHWPACQMATAADAAVPLATATATATATAGCDSDSEHFTQPWPAPDPNQFYMCPAAAATPLLLNCAPGKGFVATSDAVGCADWSVWRRQMQCEDYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMU5</accession>
    <name>A0A6I8UMU5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL45</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L45, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mRpL45</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL45 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357926.1">
        <property type="nucleotide sequence ID" value="XM_001357889.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMU5"/>
    <dbReference type="FunCoup" id="A0A6I8UMU5">
        <property type="interactions" value="1458"/>
    </dbReference>
    <dbReference type="GeneID" id="4800700"/>
    <dbReference type="KEGG" id="dpo:4800700"/>
    <dbReference type="InParanoid" id="A0A6I8UMU5"/>
    <dbReference type="OMA" id="DWAPPKD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079972">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.240:FF:000003">
        <property type="entry name" value="39S ribosomal protein L45, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051975">
        <property type="entry name" value="mtLSU_mL45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L45, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28554:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tim44-like" evidence="10">
        <location>
            <begin position="140"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="306"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001357926.1"/>
        </source>
    </evidence>
    <sequence length="352" mass="40841" checksum="5CA552685C9CB985" modified="2020-08-12" version="1">MEKLVSAKTCLKFLQVMPQAVSSGGLGVLAPALQMQQVRHRQTKHWKPEFKKLRRQKFVKIELPNFREKPEDISKEEMRSRMKERGVLPPRPWMERPFHISCTGGIFEAYVPPEGDGKKSIISSSGAKQKLEFLEKKSKSLIAVRKIRSYDESFSSDQFGAEAQEIYVAAHTLMAAKEKYKIREYVSERCYPEMMHNVKDKTIHWRFLQSLEPPRVVHARCTEVITKENQFAQVTVRFHTQQMLAIYDRFGRLMHGSEILTKDVLEYVVFEKHISNEYGKWRLHDKIIPDWLPAKQPSPITYRLIEDVEEEPPKELSAGEEPKPLEAGSEQPKEQLQLDTPPERSSKPTLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMZ5</accession>
    <name>A0A6I8UMZ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase Rnf220</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RNF220</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358124.2">
        <property type="nucleotide sequence ID" value="XM_001358087.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMZ5"/>
    <dbReference type="FunCoup" id="A0A6I8UMZ5">
        <property type="interactions" value="1540"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800938"/>
    <dbReference type="InParanoid" id="A0A6I8UMZ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16563">
        <property type="entry name" value="RING-HC_RNF220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052443">
        <property type="entry name" value="E3_ubiq-ligase_RNF220-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031824">
        <property type="entry name" value="RNF220_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040178">
        <property type="entry name" value="RNF220_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13459:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15926">
        <property type="entry name" value="RNF220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="331"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="49"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="256"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001358124.2"/>
        </source>
    </evidence>
    <sequence length="383" mass="42356" checksum="EA27A352D297D2C3" modified="2020-08-12" version="1">MATDTVSNGENYHLPSDDDVICPTCNVKIKRCQVADHCQVEMERLHTVVPPISTPPTSTLTTTTTTSTSQSGSGGRQPWSVFQRVQKNRQTRQRQRTRKRPSSPPAPVAAPPEPSTSACPVCNKNFPQGRIQEHANECLRNSRRNGQANGERHSSDDTDDSEEYEEYEWAGQKRIRVSTLVQGGYSALNMGQTIKYNGSQQANEDEDEDLNVDEDDTQIYGPTQYGEADVIPTVDGESGGDVSEADVTNYVRRLISSNEAPKSNHSSDATATVEAAVEQQPGTESAAASSREAPSTSRSTRSASQNQQLVIEALRAKLRLYENQSQSKFKCLICLDDYKNPAISVSCWHVHCEQCWLQTLGARKLCPQCNSITTPKDLRRIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN07</accession>
    <name>A0A6I8UN07_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Fatty-acid amide hydrolase 2-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4800953</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358135.3">
        <property type="nucleotide sequence ID" value="XM_001358098.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN07"/>
    <dbReference type="KEGG" id="dpo:4800953"/>
    <dbReference type="InParanoid" id="A0A6I8UN07"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF3">
        <property type="entry name" value="AT07710P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="65"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358135.3"/>
        </source>
    </evidence>
    <sequence length="533" mass="58323" checksum="1C3F6807C43FAFF6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNE3</accession>
    <name>A0A6I8UNE3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Organic cation transporter-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4801062</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358224.4">
        <property type="nucleotide sequence ID" value="XM_001358187.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNE3"/>
    <dbReference type="KEGG" id="dpo:4801062"/>
    <dbReference type="InParanoid" id="A0A6I8UNE3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="526"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="67"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001358224.4"/>
        </source>
    </evidence>
    <sequence length="599" mass="67061" checksum="121BFCD4FDB94D28" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNQ1</accession>
    <name>A0A6I8UNQ1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">High-affinity choline transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ChT</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
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        <property type="nucleotide sequence ID" value="XM_001358417.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNQ1"/>
    <dbReference type="FunCoup" id="A0A6I8UNQ1">
        <property type="interactions" value="58"/>
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    <dbReference type="KEGG" id="dpo:4801345"/>
    <dbReference type="InParanoid" id="A0A6I8UNQ1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005307">
        <property type="term" value="F:choline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008292">
        <property type="term" value="P:acetylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11474">
        <property type="entry name" value="SLC5sbd_CHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000008">
        <property type="entry name" value="High affinity choline transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052244">
        <property type="entry name" value="Choline_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45897">
        <property type="entry name" value="HIGH-AFFINITY CHOLINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45897:SF4">
        <property type="entry name" value="HIGH-AFFINITY CHOLINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="319"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="582"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001358454.2"/>
        </source>
    </evidence>
    <sequence length="608" mass="66417" checksum="920EF51344F5BBF1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP38</accession>
    <name>A0A6I8UP38_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 16">Alkaline phosphatase</fullName>
            <ecNumber evidence="3 16">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Alp5</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3 16"/>
    <dbReference type="RefSeq" id="XP_001358611.3">
        <property type="nucleotide sequence ID" value="XM_001358574.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP38"/>
    <dbReference type="FunCoup" id="A0A6I8UP38">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801537"/>
    <dbReference type="InParanoid" id="A0A6I8UP38"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000008">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF85">
        <property type="entry name" value="ALKALINE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 14 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="8 14 16" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026244704" description="Alkaline phosphatase" evidence="17">
        <location>
            <begin position="27"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="13">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001358611.3"/>
        </source>
    </evidence>
    <sequence length="525" mass="57390" checksum="6455F6485C8BA453" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQV8</accession>
    <name>A0A6I8UQV8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Ttc1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts with the GAP domain of NF1. Interacts (via TPR repeats) with HSP90AA1 and HSPA8.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359447.3">
        <property type="nucleotide sequence ID" value="XM_001359410.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQV8"/>
    <dbReference type="FunCoup" id="A0A6I8UQV8">
        <property type="interactions" value="2017"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802549"/>
    <dbReference type="InParanoid" id="A0A6I8UQV8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000367">
        <property type="entry name" value="Tetratricopeptide repeat domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052769">
        <property type="entry name" value="TPR_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46014">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46014:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="174"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359447.3"/>
        </source>
    </evidence>
    <sequence length="274" mass="30772" checksum="13B228477ACED5AC" modified="2020-08-12" version="1">MADKAISDDEFHDALTGETTCEKEAKKSQLNEKEIDEIVEKQSTLQLDDDEEEGAAAATVASGGDGDQEVKQPDGEISLEELRELEKHLSVEELAANKEKAAKLKLEGNELFKNDNAQRAIEIYTEGLNVCPSDSSKERAVLYGNRAAAKIKLESNKSAIDDCTKAIELWPEYVRVLLRRAKLYEQDDKPDEALEDYKKVYEIDPGQPEAREAQVRLPPIINERNEKLKTEMMANLKDLGNMILKPFGLSTQNFQMNQDPNTGSYSFNFNQNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URJ1</accession>
    <name>A0A6I8URJ1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inner dynein arm light chain, axonemal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Dnali1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359639.1">
        <property type="nucleotide sequence ID" value="XM_001359602.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URJ1"/>
    <dbReference type="FunCoup" id="A0A6I8URJ1">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="GeneID" id="4802794"/>
    <dbReference type="KEGG" id="dpo:4802794"/>
    <dbReference type="InParanoid" id="A0A6I8URJ1"/>
    <dbReference type="OMA" id="QVTIICA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079990">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="170"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359639.1"/>
        </source>
    </evidence>
    <sequence length="249" mass="28786" checksum="FA2A1A3BFD9F558C" modified="2020-08-12" version="1">MDELEITSVGQFQTLVRYNNPVLVVKHPEKKGAPLTELEMKRPQTAGALLDTKRETEEILNSILPPRCWEEDGQLWQQSVSSTPATRQDVINLQEMLDTRLQQTQARETGICPVRRELYSQCFDEIIRQVTINCSERGLLLLRIRDEIAMSMEAYETLYCSSVAFGMRKALQAHEEKEMLRDRVKTLEMEKEALEEIIADMKLKQEQAERRNAELRASEEKKFTEEITFLKKTNAQLKAQLEGITAPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US45</accession>
    <name>A0A6I8US45_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mRpS9</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359797.3">
        <property type="nucleotide sequence ID" value="XM_001359760.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US45"/>
    <dbReference type="FunCoup" id="A0A6I8US45">
        <property type="interactions" value="935"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802990"/>
    <dbReference type="InParanoid" id="A0A6I8US45"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000035">
        <property type="entry name" value="28S ribosomal protein S9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359797.3"/>
        </source>
    </evidence>
    <sequence length="400" mass="45706" checksum="D834079E2D2A5E88" modified="2020-08-12" version="1">MSKTMALRVFGLNLVKTNRSLLQNACKATALPAGQTVAEASYATEVTVHASPAAIQKQKVSKAMKAYLKRATEHDEFMKTQHLEFQIGKRHLANMMGADAETFTQEDIDEAISYLFPSGLYDPKARPAMKTPEEVFPARKAAEFDETGRPFHSMFYTGKPNFFQLLHEIVEETNKLYELEERMLRRGIKPDENQKLEIAGFQVLPKDQLETQLVESIADIEYTNFSNSMDRLIASPYAYKSKAFIERYLKPLMDQSKQLEVPKPKIDEQGRQYITTYECLRKTARADVTVRMPGTGKISINGQDVSYFEDENSREQLLFPLNYSELLGKVDVEANVEGGGPSGQSGAIRWGIAMCLRSFVDQEMIESMRLAGLLTRDYRRRERKKFGQEGARRKYTWKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU33</accession>
    <name>A0A6I8UU33_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 16">Protein kinase C</fullName>
            <ecNumber evidence="2 16">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">inaC</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">MV-25-SWS-2005</strain>
            <tissue evidence="27">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="20">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 16">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2 16"/>
    <dbReference type="RefSeq" id="XP_001360785.3">
        <property type="nucleotide sequence ID" value="XM_001360748.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU33"/>
    <dbReference type="SMR" id="A0A6I8UU33"/>
    <dbReference type="FunCoup" id="A0A6I8UU33">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804182"/>
    <dbReference type="InParanoid" id="A0A6I8UU33"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079653">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UU33">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20792">
        <property type="entry name" value="C1_cPKC_nPKC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04026">
        <property type="entry name" value="C2_PKC_alpha_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05570">
        <property type="entry name" value="STKc_PKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001160">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000218">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000080">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
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        <property type="entry name" value="C1-like_sf"/>
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        <property type="entry name" value="C2_domain_sf"/>
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        <property type="entry name" value="DAG/PE-bd"/>
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        <property type="entry name" value="PKC_DAG/PE"/>
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        <property type="entry name" value="Pkinase_C"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Protein_kinase_C_a/b/g"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C1_1"/>
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        <property type="entry name" value="C2"/>
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        <property type="entry name" value="Pkinase"/>
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        <property type="entry name" value="Pkinase_C"/>
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        <property type="entry name" value="PKC_alpha"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
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        <property type="entry name" value="C1"/>
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    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ZF_DAG_PE_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10 16 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16 21" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="24">
        <location>
            <begin position="70"/>
            <end position="120"/>
        </location>
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    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="24">
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            <begin position="135"/>
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            <begin position="188"/>
            <end position="309"/>
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        <location>
            <begin position="370"/>
            <end position="628"/>
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            <begin position="629"/>
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        <location>
            <position position="494"/>
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        <location>
            <position position="220"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="20">
        <location>
            <position position="227"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
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    <feature type="binding site" evidence="20">
        <location>
            <position position="280"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="20">
        <location>
            <position position="281"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="20">
        <location>
            <position position="282"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
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        <location>
            <begin position="376"/>
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            <name>ATP</name>
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        <ligand>
            <name>ATP</name>
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        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51285"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_001360785.3"/>
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    <sequence length="699" mass="79454" checksum="1F3D828B0F06970C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWH0</accession>
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        </recommendedName>
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        <name evidence="9" type="primary">mRpL54</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
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        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL54 family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ribosomal_L37"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00033752"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035179"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001361910.3"/>
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    <sequence length="178" mass="20468" checksum="26F83747460063A9" modified="2020-08-12" version="1">MLWRGHTFAYQGWSVAAFEDYTTKQKFQQLNGYFSIKLKRNQKCINMSSLTAALAMLRQRVIAPALATSTRCYAVKPAAPAAKKKKLGKLGPIMEKKVIPVETDANKLVGYVCGSNYLKTGQDVKIKPDAEYPDWLWTLNTERIIPLDELDPNSKQYWRRLRKLALRRNNQLSKLKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWT7</accession>
    <name>A0A6I8UWT7_DROPS</name>
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        <submittedName>
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        <name evidence="2 3 4" type="primary">LOC6903342</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3 4">MV-25-SWS-2005</strain>
            <tissue evidence="2 3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132223.2">
        <property type="nucleotide sequence ID" value="XM_002132187.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035270.2">
        <property type="nucleotide sequence ID" value="XM_015179784.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035271.2">
        <property type="nucleotide sequence ID" value="XM_015179785.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWT7"/>
    <dbReference type="KEGG" id="dpo:6903342"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002132223.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015035270.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015035271.2"/>
        </source>
    </evidence>
    <sequence length="113" mass="11356" checksum="564C03973DF17FAD" modified="2020-08-12" version="1">MEGYYSGNGYGNVYSQMTMKGNGYGYCSSGNCNNGSCSNGSCTNGSCSNGSCTNGNCSNGSCSIGSCSNGSCSSGRCQRMAAGSQYGGYCYGGKCFPGRRGGSGRSGRPAWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWV0</accession>
    <name>A0A6I8UWV0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine/arginine repetitive matrix protein 5-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6903344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132225.3">
        <property type="nucleotide sequence ID" value="XM_002132189.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWV0"/>
    <dbReference type="KEGG" id="dpo:6903344"/>
    <dbReference type="InParanoid" id="A0A6I8UWV0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026039633" evidence="2">
        <location>
            <begin position="20"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002132225.3"/>
        </source>
    </evidence>
    <sequence length="262" mass="29784" checksum="AF4459D8B462F698" modified="2020-08-12" version="1">MRFLLLVCTAALLLSVTVAEDDPPKRDELEEQKSPSPPKADEPEAAKTPQKEDDPEAATSPPKADEPEAAKTPQKEDDPQAIKSPPKEDEEDDAKSPPKEDEEDDSKSPPKEDDPEVIRFPSEYGSMGNRVAQANPYPRRYWTNRRNQQEQAIGRMAKRISEMQRNYALSKAKNMTLAAKMEAFNKKLYDVTMDLRRAEQQIENCKGVDTGNPLIPQRNVNSPSIGPVYTFRYIRLLRYLRANIDRELTAVTNLKWSREKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX31</accession>
    <name>A0A6I8UX31_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132336.2">
        <property type="nucleotide sequence ID" value="XM_002132300.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX31"/>
    <dbReference type="KEGG" id="dpo:6903457"/>
    <dbReference type="InParanoid" id="A0A6I8UX31"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UX31">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132336.2"/>
        </source>
    </evidence>
    <sequence length="150" mass="17376" checksum="0EE668DCDE5FD38D" modified="2020-08-12" version="1">MNLNLDEHFKQSQGQEASSSCNTERRKRHRSLDDENTSKTRQALDIEPKKRRIEFLNLESLRYRPSRSYCTKRHLHRTAGPKPLVSKTMEDALAKNPSLAKTRRSLLNRFEAAIEKPMESVVAQYDPMAGLSNTLARLEIFQSMDIDKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY70</accession>
    <name>A0A6I8UY70_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133145.2">
        <property type="nucleotide sequence ID" value="XM_002133109.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY70"/>
    <dbReference type="KEGG" id="dpo:6902619"/>
    <dbReference type="InParanoid" id="A0A6I8UY70"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026035530" evidence="2">
        <location>
            <begin position="17"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133145.2"/>
        </source>
    </evidence>
    <sequence length="253" mass="27218" checksum="5E3113FCB0E38BEC" modified="2020-08-12" version="1">MLLPCVLLLVIGHGLCHTLPQQKLRVIRDTRNLPTDLFKPVGNGGGKKSIPNEGQNGTVILEPTDGVEFLEKTEGEVAAVSESEESESNPPWPNYPHNSFFSSNFPPPPVFNGRNVMFPPNNIRPNPSPSFNGNNFPTSSFPNNPNTFPPLSGTFHSSGLDPRFGVPYPSGTSRNPVFSGGYGGSPNSIPYPSGVSSMTPFFSGSGPIGSSSSNFQRSEHYSYTSDGNGPPQVEHNVFDSRTPRFGSSSMSHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYH6</accession>
    <name>A0A6I8UYH6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aralkylamine N-acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6902422</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dopamine + (9Z)-octadecenoyl-CoA = N-(9Z-octadecanoyl)-dopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51380"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:31883"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:51381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dopamine + acetyl-CoA = N-acetyldopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51388"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
            <dbReference type="ChEBI" id="CHEBI:125678"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:51389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dopamine + hexadecanoyl-CoA = N-hexadecanoyl-dopamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51376"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
            <dbReference type="ChEBI" id="CHEBI:134058"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:51377"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>serotonin + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = N-[(5Z,8Z,11Z,14Z)-eicosatetraenoyl]-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51396"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:132255"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:51397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>serotonin + (9Z)-octadecenoyl-CoA = N-(9Z-octadecenoyl)-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51392"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:134064"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:51393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>serotonin + acetyl-CoA = N-acetylserotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25217"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17697"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
            <dbReference type="EC" id="2.3.1.87"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:25218"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>serotonin + hexadecanoyl-CoA = N-hexadecanoyl-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51384"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:134059"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:51385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>serotonin + octadecanoyl-CoA = N-octadecanoyl-serotonin + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:134065"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:51401"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the acetyltransferase family. AANAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.87" evidence="5"/>
    <dbReference type="RefSeq" id="XP_002133356.2">
        <property type="nucleotide sequence ID" value="XM_002133320.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYH6"/>
    <dbReference type="KEGG" id="dpo:6902422"/>
    <dbReference type="InParanoid" id="A0A6I8UYH6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000046">
        <property type="entry name" value="Dopamine N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20905:SF1">
        <property type="entry name" value="AT07410P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <dbReference type="ARBA" id="ARBA00051823"/>
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            <dbReference type="ARBA" id="ARBA00052178"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00052335"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00052491"/>
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            <dbReference type="PROSITE" id="PS51186"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_002133356.2"/>
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    <sequence length="219" mass="24544" checksum="F4CBD7A2ACF6183A" modified="2020-08-12" version="1">MKASGKDGISIRTMTEKDYAAVKPFMKEHFFTGEPLGSSTGEPVHLQNEEDNDEYHLSMIRQETCLVALQGERLVGFVLAGAQCPEDVERHREEAEKMEDHAWGRICRLSSKVEGQVNLYERYGVSRVLYSHITNVAASMRGKGLGSRLAAALMEVGRAKGFPLMVAYCTSFYSARQKAALGMECIYSLSYQDYKDNAGRVIFKPAPPHTHFRVMVIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYP9</accession>
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        <submittedName>
            <fullName evidence="5">Protein wings apart-like</fullName>
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        <name evidence="5" type="primary">wapl</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <strain evidence="5">MV-25-SWS-2005</strain>
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        <property type="entry name" value="WAPL"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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            <dbReference type="ARBA" id="ARBA00006854"/>
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        <source>
            <dbReference type="PROSITE" id="PS51271"/>
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            <dbReference type="RefSeq" id="XP_002133488.2"/>
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</entry>
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    <accession>A0A6I8UYY5</accession>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein fs(1)N</fullName>
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        <name evidence="6" type="primary">fs(1)N</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <subcellularLocation>
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</entry>
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        <submittedName>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002135331.2"/>
        </source>
    </evidence>
    <sequence length="93" mass="7958" checksum="84D93D936E310DBB" modified="2020-08-12" version="1">MKLPVLLWISLALLMSMAAAQGPAEAAQAGIGAAAGAGAQIGAQAGGGADAAEGMGVGMDAGVGGGAGAGAGAGAKGGAGVGGGGGGGAKGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2M7</accession>
    <name>A0A6I8V2M7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Gametogenetin-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137349.3">
        <property type="nucleotide sequence ID" value="XM_002137313.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2M7"/>
    <dbReference type="FunCoup" id="A0A6I8V2M7">
        <property type="interactions" value="2716"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897169"/>
    <dbReference type="InParanoid" id="A0A6I8V2M7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026073">
        <property type="entry name" value="GGNBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13601">
        <property type="entry name" value="GAMETOGENETIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13601:SF2">
        <property type="entry name" value="GAMETOGENETIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="554"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137349.3"/>
        </source>
    </evidence>
    <sequence length="692" mass="78993" checksum="E3467E1FE5B3BB1E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2R0</accession>
    <name>A0A6I8V2R0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Fkbp39</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="RefSeq" id="XP_002137414.1">
        <property type="nucleotide sequence ID" value="XM_002137378.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2R0"/>
    <dbReference type="FunCoup" id="A0A6I8V2R0">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="GeneID" id="6897243"/>
    <dbReference type="KEGG" id="dpo:6897243"/>
    <dbReference type="InParanoid" id="A0A6I8V2R0"/>
    <dbReference type="OMA" id="CPPHMAY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247935">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023566">
        <property type="entry name" value="PPIase_Fpr3/Fpr4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811:SF19">
        <property type="entry name" value="39 KDA FK506-BINDING NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001473">
        <property type="entry name" value="FK506-bp_FPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="253"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002137414.1"/>
        </source>
    </evidence>
    <sequence length="341" mass="37895" checksum="5B448477F6E105ED" modified="2020-08-12" version="1">MSMFWGLVMKPNRKYTQTIVKSFHISGVALDKGEEAKLYLTAEKQKYVVATVRKDNPQVPLDLNFSKGDRIIFQTTGDATVSLLGYLHDIDSEDEDDEEFNLKTLLKGKADKNGQEDEDEDDDEEGDEDDEDIEDSKIIEEYESLLENGDEEDDEEDDEDESEEEDSEEEDEQPKAKVAKIAPKANEKAGKDQNGVAKNQPEKQAKKEKPESKKEQPKAKEQEKKPAGGERTITGGVKILDLTTGKGPEAQKGKRATVYYIGRLQSNNKTFDSMLQGKGFRFRLGGGEVIKGWDTGVIGMKVGGKRRITCPPHMAYGSRGAPPNIPGNSTLVFDVELKGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2S7</accession>
    <name>A0A6I8V2S7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Biogenesis of lysosome-related organelles complex 1 subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Muted</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137481.3">
        <property type="nucleotide sequence ID" value="XM_002137445.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2S7"/>
    <dbReference type="FunCoup" id="A0A6I8V2S7">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897329"/>
    <dbReference type="InParanoid" id="A0A6I8V2S7"/>
    <dbReference type="OMA" id="TNLQHGY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248607">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017243">
        <property type="entry name" value="Bloc1s5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31784">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31784:SF2">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14942">
        <property type="entry name" value="Muted"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002137481.3"/>
        </source>
    </evidence>
    <sequence length="154" mass="17993" checksum="11E912CE51188BE5" modified="2020-08-12" version="1">MIIQVGRELYKVPLRVLDHRVFVNGEIEAFLDNFEVRRNDSEVEKLFQVTETVGSLKYDLSERFISLGNKNLTDLGTEVNNLLYGVNNLLEKAKVERTASAQLRESRQSRERRRAEFLTNLQHGYKRIENSFEEKEEEIAELYSDLQLKLSIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V319</accession>
    <name>A0A6I8V319_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sperm-associated antigen 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137680.2">
        <property type="nucleotide sequence ID" value="XM_002137644.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V319"/>
    <dbReference type="FunCoup" id="A0A6I8V319">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897536"/>
    <dbReference type="InParanoid" id="A0A6I8V319"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043195">
        <property type="entry name" value="TTC12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137680.2"/>
        </source>
    </evidence>
    <sequence length="232" mass="27162" checksum="B3800263DEE738DE" modified="2020-08-12" version="1">MFSLLHPQTDESFLDTESTVEDVIRLLQSLQKPKEEGEEEAEAPKEDLRGESVTEDNFMVTERQKPLAPKKVPIPILKSKKKKADPSDGNKDRFTFMRQVDVDHEESSKARLERERVAQNFRKLGNSEYRQGNYLTAMRMFSKAIENINDSHILYINRALCFIKSGRFKRAIVDCDYVLNKLDEKNLRAWLYRAMAYKGLNDESNFENCVKYARKFNSKQMEFIDGFLEKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V333</accession>
    <name>A0A6I8V333_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA-binding protein 34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6897406</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM RBM34 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137558.2">
        <property type="nucleotide sequence ID" value="XM_002137522.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V333"/>
    <dbReference type="FunCoup" id="A0A6I8V333">
        <property type="interactions" value="533"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897406"/>
    <dbReference type="InParanoid" id="A0A6I8V333"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="152"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="254"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="64"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="339"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002137558.2"/>
        </source>
    </evidence>
    <sequence length="429" mass="47451" checksum="47665474F805D139" modified="2020-08-12" version="1">MKTPRKPETVQGELKPTKKQPVVSQDSKLIKKKEKRPKQLKTEKAVDNESPRKLTVADLAKIEKKRAKKEGQKLKKQQQKLLKASAGPTETEVKKEINSVEKESEEETEKLTPKTVVKQETKQQKKASKKSKANNKDEGAAKWERDPAQEAATVFVGNLPINTKRVQLVRLLKPYGNVKSIRLRTAGGKQLFKHKQRKGAGSLNAYVVLDSAEIAERALALNGTEFKENHLRVTPAAKSESGDDKGPKDADAKRTIFVGSLKYSATEEKLREIFSSCGEIEYIRCLQEGEKGCKGVAYVCFKTPDAVGLALELNQTLLDDRPINVERYLVKKLGAKRVRDAAAAKSGPPSKSKTKPNSVGAKKRLDKKQYKQNGKPTAALGDKTAAGSKKKSEYRGVKVDGLKKAKKPKKKSNDQLHAMAKKIAPKPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3T7</accession>
    <name>A0A6I8V3T7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arrestin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6897926</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138028.2">
        <property type="nucleotide sequence ID" value="XM_002137992.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3T7"/>
    <dbReference type="FunCoup" id="A0A6I8V3T7">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897926"/>
    <dbReference type="InParanoid" id="A0A6I8V3T7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF167">
        <property type="entry name" value="ARRESTIN C-TERMINAL-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="4">
        <location>
            <begin position="180"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002138028.2"/>
        </source>
    </evidence>
    <sequence length="467" mass="50739" checksum="11C774F6FF207F90" modified="2020-08-12" version="1">MSSKVTFAFDNNPLGVYHAGQVISGTAELTTDRPKTIRSIFITINGYGETRWQESVEKPGDDGKTTKSMETHTSTEIYYSSDKYVYGQSGGSQLELPKGKFVFPFHTTIPPNAPTSFNGSHGQIKHEVTLTIDRSVRYNNIFKQCFTVILPHDLNTKRENAQPLKRIEEKSFWWGSIFGANKPMVMDVSTSYSGYVPGQKIHFHLVLDNQSDVQCQDVKVRLLKNVTYRANSTNKEQLKSTESRIADMHCGEVVKHNKAQFDEYLLVPPTTPTTVGERDPIRVSYTLRFIAKTFRLHGGGDLVVDFPVTIGTLPLLGSGEDKGPGQTASSNGSVNSYPGAPIFQEDVSDEQFESSTFKPRYPVYTFGQPGNAANGAPAATPSLYPRAEDTPPVAPLPYAPNPVTPVAKPTAPGPQKIPPYPTNSPAPLPAAAPGGGHFEVLGFSIPPGYQPTPSAPGAAPPAGIGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V413</accession>
    <name>A0A6I8V413_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137629.3">
        <property type="nucleotide sequence ID" value="XM_002137593.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V413"/>
    <dbReference type="KEGG" id="dpo:6897480"/>
    <dbReference type="InParanoid" id="A0A6I8V413"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006631">
        <property type="entry name" value="DM4_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398">
        <property type="entry name" value="AGAP007094-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398:SF7">
        <property type="entry name" value="LP19941P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07841">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00718">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137629.3"/>
        </source>
    </evidence>
    <sequence length="318" mass="36055" checksum="EA97966B4B9B112E" modified="2020-08-12" version="1">MGSAGQVIIKLTTWHFDRTIKQRNRKKIGGWPSKSMAKNFKSVGKMSTTWPKLLLTAFFLGQCQSQSKNDVLHVFAPPLLSTTSHEAGKVNNVTLTPPGLITQGRELEQELELELELGQDPLTPHAALSSQRKLSRGKRFVAFPVGSSASVAVCFTVGIIGNPYYAYNSFGINWGVAYDLPNTTWVLQHLHGFSTHPVAPAVLRRRSRSMIYQKIETIVDNMGYNGRDCVLRTLCESRQYFQRTKMNMVGEMLRTIFSLPKQRIFTRELHENSDIVHYDKAYRQAHLDDCAAQYDCHFSLLELAFGKYTTPPKNYYAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4C0</accession>
    <name>A0A6I8V4C0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fanconi anemia group I protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FANCI</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138586.3">
        <property type="nucleotide sequence ID" value="XM_002138550.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V4C0">
        <property type="interactions" value="879"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898566"/>
    <dbReference type="InParanoid" id="A0A6I8V4C0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11720">
        <property type="entry name" value="FANCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026171">
        <property type="entry name" value="FANCI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029312">
        <property type="entry name" value="FANCI_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029308">
        <property type="entry name" value="FANCI_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029315">
        <property type="entry name" value="FANCI_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029313">
        <property type="entry name" value="FANCI_S3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029314">
        <property type="entry name" value="FANCI_S4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818">
        <property type="entry name" value="BC025462 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21818:SF0">
        <property type="entry name" value="FANCONI ANEMIA GROUP I PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14680">
        <property type="entry name" value="FANCI_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14675">
        <property type="entry name" value="FANCI_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14676">
        <property type="entry name" value="FANCI_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14677">
        <property type="entry name" value="FANCI_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14678">
        <property type="entry name" value="FANCI_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FANCI solenoid 1" evidence="2">
        <location>
            <begin position="67"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI solenoid 2" evidence="3">
        <location>
            <begin position="391"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI helical" evidence="6">
        <location>
            <begin position="555"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI solenoid 3" evidence="4">
        <location>
            <begin position="821"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="domain" description="FANCI solenoid 4" evidence="5">
        <location>
            <begin position="1049"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1313"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1317"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1336"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1390"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1407"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1430"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1453"/>
            <end position="1465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002138586.3"/>
        </source>
    </evidence>
    <sequence length="1465" mass="168061" checksum="68E8F3D9A12E5340" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4P2</accession>
    <name>A0A6I8V4P2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aquaporin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Eglp3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138557.1">
        <property type="nucleotide sequence ID" value="XM_002138521.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4P2"/>
    <dbReference type="FunCoup" id="A0A6I8V4P2">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="GeneID" id="6898516"/>
    <dbReference type="KEGG" id="dpo:6898516"/>
    <dbReference type="InParanoid" id="A0A6I8V4P2"/>
    <dbReference type="OMA" id="IRAIAYF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246222">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000020">
        <property type="entry name" value="Entomoglyceroporin 4, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF270">
        <property type="entry name" value="ENTOMOGLYCEROPORIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002138557.1"/>
        </source>
    </evidence>
    <sequence length="273" mass="29393" checksum="59F52610D8B3B698" modified="2020-08-12" version="1">MSQQKPKQASGFDSPSGGRWRLHQKHRSRVAAFFGELVGTAMFVLIACWGCVQTPIFNNTHFQSGLTFGLAILIAIQCYGSVSGAHLNPAITLAAALYGVLHWGMAIAYFVAQVAGAFIGYGLLVAVLPHNAIKGVDNPAGVCVTVLASDISVLQGVFIEFLITCCLVMVACSVWDPRNAKLKDSVPVRFGLTVSCLIITAGLFTGASMNPTRSLGPAVWNDSWEHHWIYWVGPLVAGAVTSLIYRFAFKGDEVIELRSSNAKIRVVEEVVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5K6</accession>
    <name>A0A6I8V5K6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein Turandot B1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4801544</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Turandot family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736545.3">
        <property type="nucleotide sequence ID" value="XM_003736497.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5K6"/>
    <dbReference type="FunCoup" id="A0A6I8V5K6">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801544"/>
    <dbReference type="InParanoid" id="A0A6I8V5K6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010825">
        <property type="entry name" value="Turandot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07240">
        <property type="entry name" value="Turandot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026134012" evidence="7">
        <location>
            <begin position="25"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_003736545.3"/>
        </source>
    </evidence>
    <sequence length="141" mass="16294" checksum="24B15F3D0F8910F3" modified="2020-08-12" version="1">MPTSSITSVFLSLLFLCLFYTGYTDNNLDEDKRKLFEIYNSSHDDMTKINNIPELVNIYERISYTLSPDLREKLDRIIDENTALDHDTILVDGIPRQGGKKKRKFIVPKVLTEPVKDVASGFFQRLGSGLYDFFFGKKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5N8</accession>
    <name>A0A6I8V5N8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC13036408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736560.2">
        <property type="nucleotide sequence ID" value="XM_003736512.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5N8"/>
    <dbReference type="FunCoup" id="A0A6I8V5N8">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="GeneID" id="13036408"/>
    <dbReference type="KEGG" id="dpo:13036408"/>
    <dbReference type="InParanoid" id="A0A6I8V5N8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0263673">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_003736560.2"/>
        </source>
    </evidence>
    <sequence length="55" mass="5348" checksum="6E26111CE5A82FFB" modified="2020-08-12" version="1">MGCCLATSKSVDLPPVPPAPGKQRSTLPEFPVPTTSAPVGGGGPSGATNAALEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6U6</accession>
    <name>A0A6I8V6U6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Persulfide dioxygenase ETHE1, mitochondrial</fullName>
            <ecNumber evidence="13">1.13.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Sulfur dioxygenase ETHE1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC15382869</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>S-sulfanylglutathione + O2 + H2O = sulfite + glutathione + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12981"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58905"/>
            <dbReference type="EC" id="1.13.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Monomer. Interacts with TST. May interact with RELA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.18" evidence="13"/>
    <dbReference type="RefSeq" id="XP_004444371.1">
        <property type="nucleotide sequence ID" value="XM_004444314.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6U6"/>
    <dbReference type="FunCoup" id="A0A6I8V6U6">
        <property type="interactions" value="806"/>
    </dbReference>
    <dbReference type="GeneID" id="15382869"/>
    <dbReference type="KEGG" id="dpo:15382869"/>
    <dbReference type="InParanoid" id="A0A6I8V6U6"/>
    <dbReference type="OMA" id="DYKGDTV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0264758">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070813">
        <property type="term" value="P:hydrogen sulfide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000013">
        <property type="entry name" value="Persulfide dioxygenase ETHE1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051682">
        <property type="entry name" value="Mito_Persulfide_Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084:SF1">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 19" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="17">
        <location>
            <begin position="76"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_004444371.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33302" checksum="ADEE3753C5803A41" modified="2020-08-12" version="1">MRIVGRSMLSALKRLSVSHQNQTILARSSSVRSAQRNPESQSQRSVHSQFGTMSLPERKPFTPDFFFRQLFDGESSTYSYLLADLKTGEAVIIDPVLEQAKRDAQLVKELGFKLKYAINTHMHADHITGSGWLRELTGCQSVIAAASGAKADCHLKEGDHVDFGSHVIDTLATPGHTNGCMSYVIKEQGCVFTGDTLLIRGCGRTDFQEGSPKNLYENVHSKIFTLPDNYRIYPAHDYKGQLESSVWEEKRYNPRLTKDLEEFIKIMENLNLPYPKKIDASLPANRECGVYDIPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V825</accession>
    <name>A0A6I8V825_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein hairy isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Sidpn</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015035770.2">
        <property type="nucleotide sequence ID" value="XM_015180284.2"/>
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    <dbReference type="FunCoup" id="A0A6I8V825">
        <property type="interactions" value="70"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V825">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11410">
        <property type="entry name" value="bHLH_O_HES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000087">
        <property type="entry name" value="Transcription factor hes-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="82"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="8">
        <location>
            <begin position="157"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="272"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="452"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015035770.2"/>
        </source>
    </evidence>
    <sequence length="528" mass="57044" checksum="045F3CA9941797B4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9X0</accession>
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        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">LOC4816380</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Pterygota</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036849.2">
        <property type="nucleotide sequence ID" value="XM_015181363.2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="149"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015036849.2"/>
        </source>
    </evidence>
    <sequence length="287" mass="33029" checksum="FFC8F8DBED22AE6F" modified="2020-08-12" version="1">MCSTHNSIRRDVVSGGQTSRALYLTEPVLCATVPRMWRGSETSFNSDSIRRFSDLEPNSSVKSSYQRSPNLKQSNRFITPLRMGWSLAIALVDQHQPIHSFTEATMPKGQNARKKENLLLGMLGFLVLFLDQWGAVAGMRCKVPYEKVQEKYCYFIADEEPLHTSFHNFCYQDKKTSRVCLDSDEEMRILARHLYEIGFQNGTQFWSAGHRWPGDMRFYWNYFGRARALNYSNWAPEEPTPLMGRNCLILTLRAGELFMSSESCYTRAVDICEQTLNTESGSRTVIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBQ6</accession>
    <name>A0A6I8VBQ6_DROPS</name>
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        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3 4" type="primary">LOC13036428</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <strain evidence="2">MV2-25</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_003736697.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037862.2">
        <property type="nucleotide sequence ID" value="XM_015182376.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:13036428"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_003736745.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015037862.2"/>
        </source>
    </evidence>
    <sequence length="82" mass="9020" checksum="4660E53F75423B1F" modified="2020-08-12" version="1">MKPSALSACIRQIWIMSGWLRHEAAAAAGMIVLRREKEQSTEDNQGEVPEAEAVGQVGVDTEGKPHKVRILDDYVVPFECGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDR9</accession>
    <name>A0A6I8VDR9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Lrrk</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_015038660.2">
        <property type="nucleotide sequence ID" value="XM_015183174.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VDR9">
        <property type="interactions" value="666"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VDR9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VDR9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
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    <sequence length="2456" mass="272831" checksum="E5B53CA40DC7C244" modified="2020-08-12" version="1"> </sequence>
</entry>
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        <source>
            <dbReference type="Pfam" id="PF13091"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015039304.1"/>
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    <sequence length="181" mass="21547" checksum="6976016FBD342715" modified="2020-08-12" version="1">MEYFKRISSMLSYCLDYILFNGWLAVFGDKLEDYHKRSNCNKRHCKNCRVQSVVKRIKRAKFHVVLAIMTISHKTLCKALMEARLRGVQVRIATPKKMLSARDLVTRQLLMYIHSVDHSEVPIIKCVFLDGNRIILRMDDELLLLMRRLLIDENLFSYYFWYFGYLWHGEYFEPLDIPTAA</sequence>
</entry>
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    <accession>A0A6I8VGA5</accession>
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        <name evidence="6" type="primary">LOC4804814</name>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">MV2-25</strain>
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                <consortium name="RefSeq"/>
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            <strain evidence="6">MV-25-SWS-2005</strain>
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        <text evidence="1">Belongs to the TPD52 family.</text>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="TUMOR PROTEIN D52"/>
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        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
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        <location>
            <begin position="345"/>
            <end position="375"/>
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    </feature>
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        <location>
            <begin position="186"/>
            <end position="213"/>
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    </feature>
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            <begin position="359"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015040212.2"/>
        </source>
    </evidence>
    <sequence length="375" mass="41609" checksum="7B4D45E71434970C" modified="2020-08-12" version="1">MSESEPEPEPVSLEFIEDPYLPRLDELTTTPSLDQDQDDDSALGADSDWYEEAETEAELYLRNLTLDQIFYDVDSDYANTLELQAVETEFVGPKLANQAPSSSMSKRFRLRFFAKRTMRDVKVTFIDFSKPYLAKISNSDNYKRFLSIGQSGQSGRDNSANLSEPASPAASVASAEIAAEFAALSTEEKEQRRAEWSQELARVEEEINTLRTVLASKTRHASDLKRKLGITVWKELTDDMNQGVKNIKESTVYQSVEESMGTFAKAVHEAPLYQRTESVLKTTGEKTASVFGSITSGITSKLSQMKNSESMRSIEASVGSAYENVKVSYEHNNFMCQSHATKVTSRSGSVSSFPDAMDENNTSSGLNSPTDSLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGL0</accession>
    <name>A0A6I8VGL0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Guanine Nucleotide-binding protein subunit gamma-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">Ggamma1</name>
        <name evidence="11" type="synonym">LOC4805047</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <strain evidence="10 11">MV2-25</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361512.1">
        <property type="nucleotide sequence ID" value="XM_001361475.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040387.1">
        <property type="nucleotide sequence ID" value="XM_015184901.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGL0"/>
    <dbReference type="SMR" id="A0A6I8VGL0"/>
    <dbReference type="GeneID" id="4805047"/>
    <dbReference type="KEGG" id="dpo:4805047"/>
    <dbReference type="OMA" id="YCQDHRR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080927">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="9">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001361512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015040387.1"/>
        </source>
    </evidence>
    <sequence length="70" mass="8178" checksum="4D0BAFDBA091B122" modified="2020-08-12" version="1">MDVMSSSLQQQRIVVDQLRREASMERQTISESCAKMMKYITEHEQEDYLLTGFTSQKVNPFREKSSCTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGM7</accession>
    <name>A0A6I8VGM7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6901882</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015040930.2">
        <property type="nucleotide sequence ID" value="XM_015185444.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGM7"/>
    <dbReference type="InParanoid" id="A0A6I8VGM7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF165">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="540"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="562"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015040930.2"/>
        </source>
    </evidence>
    <sequence length="640" mass="68322" checksum="5732A3252EAFDF2B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHP0</accession>
    <name>A0A6I8VHP0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4816016</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041091.2">
        <property type="nucleotide sequence ID" value="XM_015185605.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VHP0"/>
    <dbReference type="FunCoup" id="A0A6I8VHP0">
        <property type="interactions" value="1587"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816016"/>
    <dbReference type="InParanoid" id="A0A6I8VHP0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000003">
        <property type="entry name" value="Pyruvate dehydrogenase phosphatase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.110:FF:000004">
        <property type="entry name" value="Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032503">
        <property type="entry name" value="FAO_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF15">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE PHOSPHATASE REGULATORY SUBUNIT, MITOCHONDRIAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16350">
        <property type="entry name" value="FAO_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="70"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase central" evidence="5">
        <location>
            <begin position="433"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="GCVT N-terminal" evidence="3">
        <location>
            <begin position="490"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="4">
        <location>
            <begin position="801"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015041091.2"/>
        </source>
    </evidence>
    <sequence length="934" mass="105601" checksum="7C9CA48090232A32" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJ67</accession>
    <name>A0A6I8VJ67_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC6901723</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134391.3">
        <property type="nucleotide sequence ID" value="XM_002134355.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042074.2">
        <property type="nucleotide sequence ID" value="XM_015186588.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VJ67"/>
    <dbReference type="KEGG" id="dpo:6901723"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VJ67">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634523" evidence="3">
        <location>
            <begin position="18"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002134391.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015042074.2"/>
        </source>
    </evidence>
    <sequence length="253" mass="27803" checksum="2A80C91E4B808B24" modified="2020-08-12" version="1">MILLPVLRLSIFPCGCCFQLFHQMVSRAHAWAPPQFPRGEGHGVLALGLSARKKNRCYCQSVARVFESKMSELQQQQNSYEQECQRAELLGLQAPDKDEFDRRLQARLEQEQADHDAAEAVLLEQQGEALRDVGGKLGELNSILSSTQQRLNRFKQTACGSLTNIFARPSSGSVDLSAAAAGAAEGAASQEERAPVQEQPRHPPTAAEVSAAKAAKQQRIDSDLDKLDALINKADNAHIAMSEQTKQMRRLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VM27</accession>
    <name>A0A6I8VM27_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21 22 23" type="primary">LOC6900031</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21 22 23">MV-25-SWS-2005</strain>
            <tissue evidence="21 22 23">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_002135395.3">
        <property type="nucleotide sequence ID" value="XM_002135359.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043704.2">
        <property type="nucleotide sequence ID" value="XM_015188218.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240335.1">
        <property type="nucleotide sequence ID" value="XM_033384444.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900031"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VM27">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000001">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000002">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009398">
        <property type="entry name" value="Adcy_conserved_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF16">
        <property type="entry name" value="ADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06327">
        <property type="entry name" value="Adcy_cons_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="593"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="623"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="680"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1045"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1072"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1117"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1181"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1210"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1244"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="19">
        <location>
            <begin position="770"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="19">
        <location>
            <begin position="1331"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="355"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1526"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1790"/>
            <end position="1848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1869"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1526"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1793"/>
            <end position="1848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1872"/>
            <end position="1888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_002135395.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_015043704.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_033240335.1"/>
        </source>
    </evidence>
    <sequence length="1903" mass="208751" checksum="C5D4C151FC753CF0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMV9</accession>
    <name>A0A6I8VMV9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metalloendopeptidase</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4802171</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_033232396.1">
        <property type="nucleotide sequence ID" value="XM_033376505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VMV9"/>
    <dbReference type="FunCoup" id="A0A6I8VMV9">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802171"/>
    <dbReference type="InParanoid" id="A0A6I8VMV9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079077">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 10 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="12">
        <location>
            <begin position="113"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="155"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033232396.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="34521" checksum="3F660559D9372875" modified="2020-08-12" version="1">MLYANEYRYKVPAIKRCRLIASGSAAKSPPTIFGGLGVAGALPMVDLDSEDVIDLSDLGEAIFGNPDSETTGVLVDAHDEQSQQNPEELGTYYEGDILIPLSYREARSNGTRNGILAQSFRWPGAVVPYEIKGPFTTQELGNINHAFKEYHTRTCVRFKPRSTEKDYISIGSGKSGCWSSIGRLGGRQEVNLQSPNCLRTYGTPIHELMHALGFFHEQNRHERDSYVKVMSDNIKPDMMANFEKASSRTQSGFGVDYDYGSVMHYSSTSFTRNGQPTLKALRPSSAASQMGQRKGFSAGDVRKINAMYKCKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VP27</accession>
    <name>A0A6I8VP27_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC117183332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232782.1">
        <property type="nucleotide sequence ID" value="XM_033376891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VP27"/>
    <dbReference type="KEGG" id="dpo:117183332"/>
    <dbReference type="InParanoid" id="A0A6I8VP27"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033232782.1"/>
        </source>
    </evidence>
    <sequence length="121" mass="13938" checksum="2C0FA8893FD3562E" modified="2020-08-12" version="1">MLYDPACYHWPMQLKMLRKLSLLLANETISVMIINKSHNYLGVVFKKFSKAVNCHGATIFSTSRLDGWDFKMRPRQDSTREYLRYIAQNRQPELLGYDANGEPKAPEAALAYIECLFAESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VSN2</accession>
    <name>A0A6I8VSN2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117183632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234091.1">
        <property type="nucleotide sequence ID" value="XM_033378200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VSN2"/>
    <dbReference type="KEGG" id="dpo:117183632"/>
    <dbReference type="InParanoid" id="A0A6I8VSN2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="1">
        <location>
            <begin position="308"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5641" evidence="2">
        <location>
            <begin position="588"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="76118" checksum="71C3BB929DB38DCF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VUA9</accession>
    <name>A0A6I8VUA9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PilZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4804099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234656.1">
        <property type="nucleotide sequence ID" value="XM_033378765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VUA9"/>
    <dbReference type="KEGG" id="dpo:4804099"/>
    <dbReference type="InParanoid" id="A0A6I8VUA9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033234656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="21046" checksum="654528EB35756535" modified="2020-08-12" version="1">MFAFEQNDFELQVDNFTCSSQDTDYRILKEFRCGLNKNAKRRSWYMEFKLQEVTSEHEVFMTIVLPRRKPMTEFVLLNLTTDGCQLLSNRNQVPLMRLGRNIMERFSNFPKQCPFEGDVIYYIRGFRLDLSLLPAVEMETPVHIQFGYQAKLQGVLWLQGNLAARVQRMSEKKRSKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWI6</accession>
    <name>A0A6I8VWI6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Kelch-like protein 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">klhl10</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235445.1">
        <property type="nucleotide sequence ID" value="XM_033379554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWI6"/>
    <dbReference type="FunCoup" id="A0A6I8VWI6">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VWI6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18450">
        <property type="entry name" value="BACK_KLHL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="81"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="708"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="718"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="749"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235445.1"/>
        </source>
    </evidence>
    <sequence length="781" mass="87344" checksum="366F31C6C447DBA0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWN0</accession>
    <name>A0A6I8VWN0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein mitoshell isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mtsh</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235353.1">
        <property type="nucleotide sequence ID" value="XM_033379462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWN0"/>
    <dbReference type="InParanoid" id="A0A6I8VWN0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080587">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VWN0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235353.1"/>
        </source>
    </evidence>
    <sequence length="886" mass="99580" checksum="6A35B5AD590D1C23" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXA1</accession>
    <name>A0A6I8VXA1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polysialoglycoprotein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4801129</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235715.1">
        <property type="nucleotide sequence ID" value="XM_033379824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXA1"/>
    <dbReference type="FunCoup" id="A0A6I8VXA1">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="ESTHER" id="drops-q29ae1">
        <property type="family name" value="Insect_lipase"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801129"/>
    <dbReference type="InParanoid" id="A0A6I8VXA1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000076">
        <property type="entry name" value="phospholipase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002334">
        <property type="entry name" value="Allerg_PlipaseA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF150">
        <property type="entry name" value="FI01825P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00825">
        <property type="entry name" value="DOLALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026032101" evidence="6">
        <location>
            <begin position="34"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="7">
        <location>
            <begin position="82"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="366"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
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        <location>
            <begin position="515"/>
            <end position="550"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
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            <begin position="557"/>
            <end position="592"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
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            <begin position="760"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    <sequence length="834" mass="86258" checksum="C339F29552453470" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W031</accession>
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        </submittedName>
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        <name evidence="5" type="primary">LOC117184220</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Endopterygota</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <location>
            <begin position="1"/>
            <end position="19"/>
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    </feature>
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            <begin position="20"/>
            <end position="465"/>
        </location>
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            <begin position="173"/>
            <end position="190"/>
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            <begin position="197"/>
            <end position="215"/>
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            <begin position="314"/>
            <end position="332"/>
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            <begin position="414"/>
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    </feature>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_033236687.1"/>
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    <sequence length="465" mass="52166" checksum="3338BD6D0D36D6B2" modified="2020-08-12" version="1">MKTGTIISCCLLIFNNVCCQSGSEQKLIDPHDGWSNFVMDFDQTLGESCPQCECPEMPQRSPAAIEDALSLVYFKKFVNLLFQRKSLQYDATATLYKRSLLFSLLPSQIEDLEHVQDARDLDILLTRILERAEAAPLVEGALGCAYAHQGMGVWALVTDILKDFTQLLKISEVQFMLLAALAALAVCIVHKSFRFRLISVILGGVLLCGYFHTYLECNRKLEVDAMLDVINSHQEPLSYDEMSWMQRLRNFVVRPSAEAEQRERLRKSSKLNRTYCLPDHVFIMYINDLFLKQLELLLEKMSQTMTKLTSGLSFPYNLIAPIFLVALVGYIIKLTFKYVISPRAWGTLLHTRPGHTDTPTMLQSQASIAGRETVGDCISGENLKMLLNVIGDTQKSVKQQLPAVSGVQELMGPLEAPSAEDKKPKLDVSNNSSSSSDSKSHSRSQTEEDGFTVVDDHEEDAIHNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>Diptera</taxon>
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                <consortium name="RefSeq"/>
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            <strain evidence="17 18">MV-25-SWS-2005</strain>
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        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033237181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033237182.1"/>
        </source>
    </evidence>
    <sequence length="9083" mass="1015868" checksum="5ED7F7E417F5A668" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W2H8</accession>
    <name>A0A6I8W2H8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Translation initiation factor IF-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812587</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237575.1">
        <property type="nucleotide sequence ID" value="XM_033381684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W2H8"/>
    <dbReference type="InParanoid" id="A0A6I8W2H8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026295049" evidence="2">
        <location>
            <begin position="22"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033237575.1"/>
        </source>
    </evidence>
    <sequence length="352" mass="36489" checksum="5AD950C3928F3D01" modified="2020-08-12" version="1">MKFSAALALLVIVGFVASGDALPARKNRQYLVPGMVYYQQPAAAEWYGAAPQQRIMYMQYVQPGRTHARSTQAASALVAGETVATGTYLKDCNHAESVDELSAEGVQAEDVLAAAGAHGATSVAEAYPDEAPVVQVAIDADVVPEAEPEVAAVENDDAAKVPREYNFVSEEAAAAAVAAEEEAIAEPVAVAVEAELPAPAPIAPVAAVVPANRYLPAKKKVIVELDQSPEEDEPEVAAIEDEEAVSDDVDEDEEELVVPVKPVVKNPARRPAAKKPVKAAGPAAKPSKKPAAPLPAGTFFPVNFGGTSGGAIAIANSFSTGEGGAATSHAIAYGSPDSVRARVRPNPSKFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W2W0</accession>
    <name>A0A6I8W2W0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">trypsin</fullName>
            <ecNumber evidence="12">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18 19" type="primary">Try29F</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17 18 19">MV-25-SWS-2005</strain>
            <tissue evidence="17 18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="12"/>
    <dbReference type="RefSeq" id="XP_033237038.1">
        <property type="nucleotide sequence ID" value="XM_033381147.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237039.1">
        <property type="nucleotide sequence ID" value="XM_033381148.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237040.1">
        <property type="nucleotide sequence ID" value="XM_033381149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W2W0"/>
    <dbReference type="KEGG" id="dpo:4816143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W2W0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF97">
        <property type="entry name" value="GH13245P2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0222">Digestion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634596" description="trypsin" evidence="14">
        <location>
            <begin position="23"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="15">
        <location>
            <begin position="34"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033237038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033237039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033237040.1"/>
        </source>
    </evidence>
    <sequence length="266" mass="28790" checksum="5403E1C77D4D6ECF" modified="2020-08-12" version="1">MERFQPAICLMLLLLLVGCCLAGTARRPRLDGRIVGGQATTIEAIPYQVSLQRSYHFCGGSLIGHGWVLTAAHCTEGSAIQVSKVRIGSTRTDSGGILVGIKRVHRHPKFDTYTIDYDFSVLELEEYDKKNVTQAFIGLPKQDADIADGTAVLVSGWGNTQSTQEPTSILRAVTVPTVSQTECTKAYGSFGTITDRMLCAGLQQGGKDACQGDSGGPLAANGTLWGVVSWGYGCARPDYPGVYSRVSAVRDWIGSNSYFWRECPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4A2</accession>
    <name>A0A6I8W4A2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Venom dipeptidyl peptidase 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6901200</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238148.1">
        <property type="nucleotide sequence ID" value="XM_033382257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4A2"/>
    <dbReference type="FunCoup" id="A0A6I8W4A2">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901200"/>
    <dbReference type="InParanoid" id="A0A6I8W4A2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF135">
        <property type="entry name" value="INACTIVE DIPEPTIDYL PEPTIDASE 10-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="7">
        <location>
            <begin position="269"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="6">
        <location>
            <begin position="764"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033238148.1"/>
        </source>
    </evidence>
    <sequence length="983" mass="110222" checksum="E78D00DA5B62F3F7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4Y1</accession>
    <accession>Q2LYT8</accession>
    <name>A0A6I8W4Y1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19 20 21" type="primary">ACXD</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20 21" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19 20 21">MV-25-SWS-2005</strain>
            <tissue evidence="19 20 21">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001354035.2">
        <property type="nucleotide sequence ID" value="XM_001353999.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237759.1">
        <property type="nucleotide sequence ID" value="XM_033381868.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237760.1">
        <property type="nucleotide sequence ID" value="XM_033381869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4Y1"/>
    <dbReference type="SMR" id="A0A6I8W4Y1"/>
    <dbReference type="GeneID" id="4813255"/>
    <dbReference type="KEGG" id="dpo:4813255"/>
    <dbReference type="OMA" id="CAMFDMI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079075">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W4Y1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000024">
        <property type="entry name" value="ACXA, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF23">
        <property type="entry name" value="AT30656P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="613"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="696"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="739"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="765"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="327"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="887"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="963"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="969"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001354035.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033237759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_033237760.1"/>
        </source>
    </evidence>
    <sequence length="1166" mass="134922" checksum="1710D4DDED053B4B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5F7</accession>
    <name>A0A6I8W5F7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18 19 20">Poly [ADP-ribose] polymerase</fullName>
            <ecNumber evidence="4 19">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">Parp1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">MV-25-SWS-2005</strain>
            <tissue evidence="27">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>L-aspartyl-[protein] + NAD(+) = 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:54424"/>
            <dbReference type="Rhea" id="RHEA-COMP:9867"/>
            <dbReference type="Rhea" id="RHEA-COMP:13832"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>L-glutamyl-[protein] + NAD(+) = 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:58224"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15089"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142540"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="4 19"/>
    <dbReference type="RefSeq" id="XP_033238605.1">
        <property type="nucleotide sequence ID" value="XM_033382714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5F7"/>
    <dbReference type="FunCoup" id="A0A6I8W5F7">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897803"/>
    <dbReference type="InParanoid" id="A0A6I8W5F7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08001">
        <property type="entry name" value="WGR_PARP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.10:FF:000006">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000051">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038650">
        <property type="entry name" value="PADR1_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008288">
        <property type="entry name" value="PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049296">
        <property type="entry name" value="PARP1-like_PADR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012982">
        <property type="entry name" value="PARP1-like_PADR1_Zn_ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21728">
        <property type="entry name" value="PADR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08063">
        <property type="entry name" value="Zn_ribbon_PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000489">
        <property type="entry name" value="NAD_ADPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01335">
        <property type="entry name" value="PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52007">
        <property type="entry name" value="PADR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="14 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 19 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 19 20" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PARP-type" evidence="21">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="PARP-type" evidence="21">
        <location>
            <begin position="111"/>
            <end position="200"/>
        </location>
    </feature>
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        <location>
            <begin position="379"/>
            <end position="453"/>
        </location>
    </feature>
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        <location>
            <begin position="524"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="24">
        <location>
            <begin position="643"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="23">
        <location>
            <begin position="769"/>
            <end position="993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012020"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00071874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_033238605.1"/>
        </source>
    </evidence>
    <sequence length="993" mass="113041" checksum="DB2978156BBDCCB6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W667</accession>
    <name>A0A6I8W667_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">E3 ubiquitin ligase complex SCF subunit sconC-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117184594</name>
        <name evidence="4" type="synonym">LOC26534418</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041617.2">
        <property type="nucleotide sequence ID" value="XM_015186131.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238841.1">
        <property type="nucleotide sequence ID" value="XM_033382950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W667"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="SKP1"/>
    </dbReference>
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        <property type="entry name" value="SKP1-like"/>
    </dbReference>
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        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
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        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015041617.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033238841.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="14767" checksum="B75BA94EC1010A39" modified="2020-08-12" version="1">MTEKRLKERIQMMSSEGERIIVDRKVAKRFVGESRMGNEIEHLYFENVRSIVLRKLILWSYYHRDDPQPELSDTQVKQWYTQFFDEDQEILLELAEIADTLEIKRLSHIARQRLLDGTTPKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8E1</accession>
    <name>A0A6I8W8E1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein RunxB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RunxB</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033239643.1">
        <property type="nucleotide sequence ID" value="XM_033383752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W8E1"/>
    <dbReference type="KEGG" id="dpo:4815062"/>
    <dbReference type="InParanoid" id="A0A6I8W8E1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W8E1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001709">
        <property type="term" value="P:cell fate determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003006">
        <property type="term" value="P:developmental process involved in reproduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048592">
        <property type="term" value="P:eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.720:FF:000001">
        <property type="entry name" value="Runt-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000040">
        <property type="entry name" value="AML1_Runt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013524">
        <property type="entry name" value="Runt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950">
        <property type="entry name" value="RUNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950:SF48">
        <property type="entry name" value="RUNT RELATED B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00853">
        <property type="entry name" value="Runt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00967">
        <property type="entry name" value="ONCOGENEAML1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Runt" evidence="6">
        <location>
            <begin position="118"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="328"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="580"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="619"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="16"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="355"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="414"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="433"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="580"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="620"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="656"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51062"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033239643.1"/>
        </source>
    </evidence>
    <sequence length="692" mass="73229" checksum="F6AEEAE2C5C6F9FA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W968</accession>
    <name>A0A6I8W968_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein 69-like isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC117184903</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239933.1">
        <property type="nucleotide sequence ID" value="XM_033384042.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239933.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="22637" checksum="718D093C3812A585" modified="2020-08-12" version="1">MDQETLMNAVNPLISKCQIASSTGILDLNDSQLTEIPDEVFNFMSNAGTEIKHCDLSGNLMEEIQPKLALDFKFITNLNIAHNLLATLPKEIGNLRALESLDISCNYLFELPDVVFKLPHLIYLYAQDNFITEIDVEKPIESDNLALVDLRYNTLEPEFLRKLMNTPTTFRLVLNDADMTENDFDDDDDQDDDDGFIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9S3</accession>
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        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="primary">LOC117184929</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240037.1">
        <property type="nucleotide sequence ID" value="XM_033384146.1"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF5641"/>
    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="125"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240037.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="26418" checksum="A9F61C8171DA8165" modified="2020-08-12" version="1">MELKRLVLSDDHQKALLDFCLVEAIDWHFIPPRSPHFGGLWEAAVKTAKYHFYRAIGNSVLGFDELRTLLCLITAVINSRPLVSNSESPADLDVLTPAHFLNGGPPASFAEPDVTQLNFDRLDGWQRVSYLQQLFWSRWKEEYITSLQQRSKWRTAKPSLAVADLVLVKDENLPPMKWPLARVVELLPGRDGVSRVAVLKTSSGIIKRSVNKLCLLPLKDAVESQASNGGSMSGQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WA22</accession>
    <name>A0A6I8WA22_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240245.1">
        <property type="nucleotide sequence ID" value="XM_033384354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WA22"/>
    <dbReference type="KEGG" id="dpo:117184996"/>
    <dbReference type="InParanoid" id="A0A6I8WA22"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240245.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="42890" checksum="9694DFCCA1997DAB" modified="2020-08-12" version="1">MKRLRDGRQARPLGANGDTTGHPSGDQRPEVPAPADEPTASRTRDWIVNPDGPSTSRQAQLRRVGEATLRQPGWMPWGQRAAAIQGWIDQQTHDPRTDAEGEVEPDGRFATAGVRSALNHDVAALNRLWLGSYGAAIDDDNATTDTDEHETILRNAPSLASTLPYEVSGHPVARGSEWDSPLWAESDLAGQGRLGTPPAVRNEDESGDGAGPHYSDVSNASDAGEGTGPQRDDGGSDSRSLMDLDFAPQANQHWAPANPSGSADIPPPFFAAHAGPPPQRPDEEVRDFGSEESLPLEYGEMADLLQILRLHPTVAAVQDDPEWAEAPPEWRTNAPGRRLPRDLISNITVFLRDRAYRLGVRRLEVHDGTCFRIKITRSRAVTVTLRHPRSMEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WA65</accession>
    <name>A0A6I8WA65_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117185006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240291.1">
        <property type="nucleotide sequence ID" value="XM_033384400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WA65"/>
    <dbReference type="KEGG" id="dpo:117185006"/>
    <dbReference type="InParanoid" id="A0A6I8WA65"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WA65">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16021">
        <property type="entry name" value="ALP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000017">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF9">
        <property type="entry name" value="DUF229 DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240291.1"/>
        </source>
    </evidence>
    <sequence length="726" mass="82499" checksum="140A321599382AD3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAU5</accession>
    <name>A0A6I8WAU5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TMEM205-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6901082</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033240392.1">
        <property type="nucleotide sequence ID" value="XM_033384501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WAU5"/>
    <dbReference type="KEGG" id="dpo:6901082"/>
    <dbReference type="InParanoid" id="A0A6I8WAU5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WAU5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025423">
        <property type="entry name" value="TMEM205-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053009">
        <property type="entry name" value="Xanthocillin_Biosynth-Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PLANTS LEA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241:SF102">
        <property type="entry name" value="LD23009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13664">
        <property type="entry name" value="DUF4149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM205-like" evidence="8">
        <location>
            <begin position="318"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="181"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033240392.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="52831" checksum="160C436D8299DAB3" modified="2020-08-12" version="1">MCHNSGIEKGEQQIERYMAFGLPSRAVKSVKYTMRKREKQQQKKEQQKLRQRQQQQEQYLEELELSSSSGSGSGSGSNNYSDSEDSGTDITSMQLASAEAAQMVDTDTDTDPEPEAAETKAEIVTYGAAAVAVTAAVAVAVAVAENLTLTETHNTKGSSIDNSSSNNNSEIFKIPKMCGRFRKLQQQQQQQQEQQQQVEDISQLKAQQQRKLSSMEPSMDDALAIGTQMTRKVSERIACLVAQLQGSRLYTILTRTTQPAHLIAVCILVFVLMTVGRDLIEGSPATTPTTTTRTLSSSSSTGGGGGSGTLPNLVYLGAFATHFGSQIWMTFVSGLSLYFSLPRHIFGQCQQILFPRYFAFNAVLSLTMLVTYAKYFLSGWTTASGIQVGSLALAAGIEVVVRLYLVPPMLKLMHEKYKIEDAIGSGQEVGSLVQGDLIDCPHYQRVHKGFRRIHMTIAIGNMTVMLATCLQLYFLASKIHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDC2</accession>
    <name>A0A6I8WDC2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Neurogenic protein mastermind-like isoform X5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6902245</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241388.1">
        <property type="nucleotide sequence ID" value="XM_033385497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDC2"/>
    <dbReference type="InParanoid" id="A0A6I8WDC2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF2">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="10">
        <location>
            <begin position="97"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="312"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="661"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="741"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="887"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="563"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="404"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="741"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="771"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="887"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033241388.1"/>
        </source>
    </evidence>
    <sequence length="942" mass="100581" checksum="6EC28448A880461B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDE8</accession>
    <name>A0A6I8WDE8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fibrillin-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">eater</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">MV2-25</strain>
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            <authorList>
                <consortium name="RefSeq"/>
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            <strain evidence="5">MV-25-SWS-2005</strain>
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    </reference>
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</entry>
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        <source>
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            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_002132633.1"/>
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</entry>
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        <submittedName>
            <fullName evidence="10 11">Cytochrome c oxidase copper chaperone</fullName>
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        <name evidence="10 11" type="primary">Cox17</name>
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            <taxon>Drosophilidae</taxon>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
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        <subcellularLocation>
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        <text evidence="2">Belongs to the COX17 family.</text>
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    </dbReference>
    <dbReference type="Bgee" id="FBgn0244308">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 8" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015041005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033241305.1"/>
        </source>
    </evidence>
    <sequence length="76" mass="7891" checksum="F68D4533D3364CFA" modified="2008-10-14" version="1">MGSSTSKGVEVAQAAASAPVAATNAASGAKPKCKACCACPETKRARDQCMVERGEENCTELIEAHKKCMRDAGFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPA2</accession>
    <accession>A0A6I8V0B4</accession>
    <name>B5DPA2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="2">6.3.5.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glutamine-dependent asparagine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">AsnS</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="2"/>
    <dbReference type="RefSeq" id="XP_002134727.1">
        <property type="nucleotide sequence ID" value="XM_002134691.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPA2"/>
    <dbReference type="SMR" id="B5DPA2"/>
    <dbReference type="FunCoup" id="B5DPA2">
        <property type="interactions" value="689"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DPA2"/>
    <dbReference type="GeneID" id="6900934"/>
    <dbReference type="KEGG" id="dpo:6900934"/>
    <dbReference type="eggNOG" id="KOG0571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_2_2_1"/>
    <dbReference type="InParanoid" id="B5DPA2"/>
    <dbReference type="OMA" id="QFASKHT"/>
    <dbReference type="UniPathway" id="UPA00134">
        <property type="reaction ID" value="UER00195"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250678">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070981">
        <property type="term" value="P:L-asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000031">
        <property type="entry name" value="Asparagine synthase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000106">
        <property type="entry name" value="Asparagine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006949">
        <property type="entry name" value="PRK09431.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="For GATase activity" evidence="12">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="352"/>
            <end position="353"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="14">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002134727.1"/>
        </source>
    </evidence>
    <sequence length="563" mass="63681" checksum="A1EA1F8570D2B30E" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DJ7</accession>
    <accession>A0A6I8W7L3</accession>
    <name>Q29DJ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8 9">Organic solute transporter alpha-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8 9 10 11 12 13 14 15" type="primary">LOC4812626</name>
    </gene>
    <organism evidence="6 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8 9">MV-25-SWS-2005</strain>
            <tissue evidence="7 8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352916.1">
        <property type="nucleotide sequence ID" value="XM_001352880.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044000.1">
        <property type="nucleotide sequence ID" value="XM_015188514.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044001.1">
        <property type="nucleotide sequence ID" value="XM_015188515.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044003.1">
        <property type="nucleotide sequence ID" value="XM_015188517.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239362.1">
        <property type="nucleotide sequence ID" value="XM_033383471.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239363.1">
        <property type="nucleotide sequence ID" value="XM_033383472.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239364.1">
        <property type="nucleotide sequence ID" value="XM_033383473.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239365.1">
        <property type="nucleotide sequence ID" value="XM_033383474.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239366.1">
        <property type="nucleotide sequence ID" value="XM_033383475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DJ7"/>
    <dbReference type="STRING" id="46245.Q29DJ7"/>
    <dbReference type="GeneID" id="4812626"/>
    <dbReference type="KEGG" id="dpo:4812626"/>
    <dbReference type="eggNOG" id="KOG2641">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063543_0_0_1"/>
    <dbReference type="OMA" id="CLPMVIP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079890">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DJ7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001352916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015044000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015044001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015044003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033239362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033239363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033239364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033239365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033239366.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="38489" checksum="3FD0648F41A3C85E" modified="2006-04-04" version="1">MNASDEWTLVEQTEPAPQEVEMEMAMEKNGSSMSLRTHPTVAEYYGNMTAFLSLAIFIASLLTILNICIYSTTVSRLRRHLNKPLRTPSILMVSLYPIISLAALATILVPYSWFICHTVMHVMFMVGGPIFRTLLFRYVDSEQNYVKESGGEAVQLTTPPCCCWCLCLPMVVPTKAKLCISRYMVWQMPFWQGSIMLVMNILYYRDIQLYHQVMYFFIPFIISSIVLGVWSLQITVRMITKLRGDYQLRKKMFCLQLVVMLCKLQYLVLYDQLDGIKMGGEYPINHTVYKQTIINILILVEMVLVSMMVQSAYRTPIQVQIDEVNKDKEITRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EJ0</accession>
    <accession>A0A6I8VM16</accession>
    <name>Q29EJ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">1-acyl-sn-glycerol-3-phosphate acyltransferase gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">Agpat4</name>
        <name evidence="7" type="synonym">LOC4812416</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352574.2">
        <property type="nucleotide sequence ID" value="XM_001352538.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043692.1">
        <property type="nucleotide sequence ID" value="XM_015188206.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EJ0"/>
    <dbReference type="GeneID" id="4812416"/>
    <dbReference type="KEGG" id="dpo:4812416"/>
    <dbReference type="eggNOG" id="KOG1505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041844_5_2_1"/>
    <dbReference type="OMA" id="EQECSTW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078408">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF24">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 3, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="5">
        <location>
            <begin position="87"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001352574.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015043692.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="44104" checksum="8655A8FE41574E37" modified="2008-10-14" version="2">MGKLTGLGRLLIAVTFFTCGFFVNIAQLFLHIFVKPFDKRLCRGLMYYLTYSFYCILVCVAEWYAGSKVRVYIDPEDEKKYFAKEHGLLLMNHTYEIDWLTAWMVTDKFGNLGNTKAYAKKMLRYVPILGWVWWMAEFIFLDRNFDKDKIVIKEQLKVVFSYPDPVWLLLNAEGTRYTAAKHELSVKFAQERGLPVLKHHLIPRTKGFTTSLPTLRGICPAIYDINLAFKRDAKVKPSMLSQLNGETVEPYMYIRRVPLSNVPEDEKEAAAWMQEFFMEKDRIIDSFHETGSFFKTSGVKEVPMKIYKPRLATLLNFIGWATVCVSCILYYMITSFIAGNWIGFFTVISILGLFYGLMEHAVNASKISKSSSYGATGEKQAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q29ET0</accession>
    <accession>A0A6I8UAR4</accession>
    <name>Q29ET0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SWI/SNF chromatin-remodeling complex subunit SNF5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352483.2">
        <property type="nucleotide sequence ID" value="XM_001352447.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29ET0"/>
    <dbReference type="FunCoup" id="Q29ET0">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29ET0"/>
    <dbReference type="GeneID" id="4812505"/>
    <dbReference type="KEGG" id="dpo:4812505"/>
    <dbReference type="eggNOG" id="KOG3308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_866759_0_0_1"/>
    <dbReference type="InParanoid" id="Q29ET0"/>
    <dbReference type="OMA" id="LQRIVHY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071393">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29ET0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352483.2"/>
        </source>
    </evidence>
    <sequence length="335" mass="39312" checksum="F4F3703AA5EBBF59" modified="2008-10-14" version="2">MPQWLVIGISGVTCGGKTTLAHSLHEYFKGLSGAPLWNSPYTIGDVQLISQDDYFLPVEDRRHTRNEALKVINFELLTSLDMSRMLNDIAQIIKGRHLAPEPAEQLVTYANFEHYALQFQQQYHQHHYNANYYKQGGAVNGAYQYVPQQQQQRQHHHPQIHQQQQQQQQQLQQQQQHAAHIMRLNAQFAAQLRDKKINFLLVEGFMIFNQPELLALCNIKYHFHLPYEKCFERRRKRTYDPPDVTGYFELCVWPHYEKNFSEYRDCKEITFLNGEISRDKIFKYVLQRIVHYFEERCDVPAAAPPACPPQQKRFGMLYVGSSNNNVLPTACPMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FA0</accession>
    <accession>A0A6I8UEQ4</accession>
    <name>Q29FA0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RpS17</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354302.2">
        <property type="nucleotide sequence ID" value="XM_001354266.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FA0"/>
    <dbReference type="SMR" id="Q29FA0"/>
    <dbReference type="FunCoup" id="Q29FA0">
        <property type="interactions" value="1142"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FA0"/>
    <dbReference type="GeneID" id="4814065"/>
    <dbReference type="KEGG" id="dpo:4814065"/>
    <dbReference type="eggNOG" id="KOG0187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112958_1_1_1"/>
    <dbReference type="InParanoid" id="Q29FA0"/>
    <dbReference type="OMA" id="MKRIQQG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077785">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FA0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002242">
        <property type="entry name" value="PRK01151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001354302.2"/>
        </source>
    </evidence>
    <sequence length="131" mass="15343" checksum="9AF83A81045029B2" modified="2008-10-14" version="2">MGRVRTKTIKKAAKVIIEKYYTRLTMDFHTNKRICEEVAIIPTKPLRNKIAGYVTHLMGRLRHSQVRGISIKLQEEERERRDNYVPAVSALEQDIIEVDPDTKEMLKLLDFHNIRGLQLTQPNTNNFGRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FM0</accession>
    <accession>A0A6I8UEM7</accession>
    <name>Q29FM0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Integrator complex subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">IntS4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354327.1">
        <property type="nucleotide sequence ID" value="XM_001354291.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FM0"/>
    <dbReference type="SMR" id="Q29FM0"/>
    <dbReference type="FunCoup" id="Q29FM0">
        <property type="interactions" value="2576"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FM0"/>
    <dbReference type="GeneID" id="4814202"/>
    <dbReference type="KEGG" id="dpo:4814202"/>
    <dbReference type="eggNOG" id="KOG2259">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012910_1_0_1"/>
    <dbReference type="InParanoid" id="Q29FM0"/>
    <dbReference type="OMA" id="GNRHPDY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071461">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000728">
        <property type="entry name" value="Blast:Integrator complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056235">
        <property type="entry name" value="INTS4_8HBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057412">
        <property type="entry name" value="INTS4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938:SF0">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24493">
        <property type="entry name" value="INTS4_8HBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25458">
        <property type="entry name" value="INTS4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INTS4 8 helical bundle" evidence="3">
        <location>
            <begin position="667"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 4/Protein SIEL C-terminal Ig-like" evidence="4">
        <location>
            <begin position="898"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001354327.1"/>
        </source>
    </evidence>
    <sequence length="1025" mass="113537" checksum="180088D28E75C9E2" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29G80</accession>
    <accession>A0A6I8UGL4</accession>
    <name>Q29G80_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Torsin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Torsin</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpA/ClpB family. Torsin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355173.3">
        <property type="nucleotide sequence ID" value="XM_001355137.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29G80"/>
    <dbReference type="FunCoup" id="Q29G80">
        <property type="interactions" value="1176"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29G80"/>
    <dbReference type="GeneID" id="4815138"/>
    <dbReference type="KEGG" id="dpo:4815138"/>
    <dbReference type="eggNOG" id="KOG2170">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053537_0_0_1"/>
    <dbReference type="InParanoid" id="Q29G80"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075746">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29G80">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049337">
        <property type="entry name" value="TOR1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010448">
        <property type="entry name" value="Torsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760:SF2">
        <property type="entry name" value="LD13476P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760">
        <property type="entry name" value="TORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21376">
        <property type="entry name" value="TOR1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06309">
        <property type="entry name" value="Torsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Torsin-1A C-terminal" evidence="2">
        <location>
            <begin position="281"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355173.3"/>
        </source>
    </evidence>
    <sequence length="339" mass="37908" checksum="FA08E64A8003ABE7" modified="2016-01-20" version="3">MNRKNVVSFLQFCFGALILWVRCSHSMEPMSIGAAIGAASVGISYFKGQTYCRLYECCDDRSIPANVQALEKSLQEKVYGQHIAVPNIISALSAHFSSRVKSRKPLVLSFHGGPGTGKSFVADQIAQALYLQGSKSEYVAKFLGRANFAQAAHVATYKEHIDREVHKRLTNCPRSLFIFDEVEKMPSGVFDTLKALLDYNGLDDEIDNTQAIFIFLSNNGGTHIAEHLGNRMKGGKLREDTRLSDYEPLLQKVAYSMDGGLKKTSLIESFVIDHYIPFLPLEKAHVLKCLEAEFQRWKKTPDRNIINDILSSMTYDRIHALFVSSGCKTLDKKVAMAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q29H95</accession>
    <accession>A0A6I8UFU3</accession>
    <name>Q29H95_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BLOC-1-related complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BORCS5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORCS5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354808.1">
        <property type="nucleotide sequence ID" value="XM_001354772.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29H95"/>
    <dbReference type="FunCoup" id="Q29H95">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29H95"/>
    <dbReference type="GeneID" id="4815165"/>
    <dbReference type="KEGG" id="dpo:4815165"/>
    <dbReference type="eggNOG" id="KOG4515">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070425_0_0_1"/>
    <dbReference type="InParanoid" id="Q29H95"/>
    <dbReference type="OMA" id="RMQTHLN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071263">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098574">
        <property type="term" value="C:cytoplasmic side of lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072384">
        <property type="term" value="P:organelle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903744">
        <property type="term" value="P:positive regulation of anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22789">
        <property type="entry name" value="BORCS5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018780">
        <property type="entry name" value="TBORCS5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31634">
        <property type="entry name" value="BLOC-1-RELATED COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31634:SF2">
        <property type="entry name" value="BLOC-1-RELATED COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10158">
        <property type="entry name" value="LOH1CR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="81"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354808.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="36886" checksum="0457166743CF6850" modified="2006-04-04" version="1">MGAEHSQQRAEADGHEIFEGRHMPGGGGLHDGSSMALAAAAGTAAANKRRGGGLNRQQTVPGTGPASIVIASKQIIADASSPASSELSRPPSPPLSVCSDLPYVSYTDRPIGDSPKIRSKPAHLLQQSSASRAANRKSHPGGFTTSSKPKRPQSTVSSHSIVIVKPGARDTNDDIDPDLARLRSIPQFLPVLRESISSATYTRDAEILERLHSQHLINICTRMQTHLNLCACYVSSEQNHLVERTKVVSNSITTLFASFVDMQKTYASYAEQFAKIRSVSHQLSRCNSLLHENIASLEAINNFLDDEDRLEPFVWRTDDNKLRGEAEGATGGNSSRLWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q29ID3</accession>
    <accession>A0A6I8UHW5</accession>
    <name>Q29ID3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocon-associated protein subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Signal sequence receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">l(1)G0320</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma. Interacts with palmitoylated calnexin (CALX), the interaction is required for efficient folding of glycosylated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAP-alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355661.1">
        <property type="nucleotide sequence ID" value="XM_001355625.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29ID3"/>
    <dbReference type="FunCoup" id="Q29ID3">
        <property type="interactions" value="1927"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29ID3"/>
    <dbReference type="GeneID" id="4815591"/>
    <dbReference type="KEGG" id="dpo:4815591"/>
    <dbReference type="eggNOG" id="KOG1631">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073618_0_0_1"/>
    <dbReference type="InParanoid" id="Q29ID3"/>
    <dbReference type="OMA" id="TFPYSFT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077101">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF0">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043735915" description="Translocon-associated protein subunit alpha" evidence="14">
        <location>
            <begin position="21"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="258"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001355661.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="33095" checksum="72823AF437F27E58" modified="2006-04-04" version="1">MKNLMFLMLMVLPIVICTFSAPNKFGAYAAEAVEDDLVDVDSEEGAVTGEDADADEDTDKESSASPNADTYLLFTKPLYTPGQQLDLPGGKPVEFLIGFTNKGAEEFVIETVEASFRYPMDFNYFIQNFSAVAYNREVKPGFESTVSYTFLPSDQFAGRPFGLNIALAYRDANGIQYNEAVFNETVLISEVDEGLDGETFFLYVLLAGIVVLLLVIGQQYLLGSSGKRKRAAAKKVIETGTANDSTIDYDWVPQETLRALQKSPPKSKTPKTSPKPGKQTSPKAASQQSPKQRKVKRSAGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IU6</accession>
    <accession>A0A6I8VH30</accession>
    <name>Q29IU6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cell differentiation protein RQCD1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">Rcd-1</name>
        <name evidence="15" type="synonym">LOC4815478</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15 16">MV-25-SWS-2005</strain>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355498.1">
        <property type="nucleotide sequence ID" value="XM_001355462.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015041181.1">
        <property type="nucleotide sequence ID" value="XM_015185695.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IU6"/>
    <dbReference type="SMR" id="Q29IU6"/>
    <dbReference type="STRING" id="46245.Q29IU6"/>
    <dbReference type="GeneID" id="4815478"/>
    <dbReference type="KEGG" id="dpo:4815478"/>
    <dbReference type="eggNOG" id="KOG3036">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039962_2_0_1"/>
    <dbReference type="OMA" id="EKVYTWI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072873">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29IU6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000037">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001355498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015041181.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33175" checksum="264CE3F504365FF3" modified="2006-04-04" version="1">MSAQPSPCMNPQQQQSEQEKVYQWINELAHPDTRETALLELSKKRETDLAPMLWNSFGTACALLQEIVNIYPSITPPTLTAHQSNRVCNALALLQCVASHPETRTAFLQAQIPLYLYPFLSTTSKTRPFEYLRLTSLGVIGALVKTDEQEVITFLLTTEIVPLCLSIMDSGSELSKTVATFIIQKILLDESGLSYICQTYERFSHVAITLGKMVIQLAKEPCARLLKHVVRCYLRLSDNTRARKALGQCLPDQLRDGTFALCLQDDKSTKQWLQMLLKNLELGNTPQQIGMSPLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q29J99</accession>
    <accession>A0A6I8UGZ5</accession>
    <name>Q29J99_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alkyl transferase</fullName>
            <ecNumber evidence="5">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Dhdds</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="5"/>
    <dbReference type="RefSeq" id="XP_001355345.1">
        <property type="nucleotide sequence ID" value="XM_001355309.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29J99"/>
    <dbReference type="FunCoup" id="Q29J99">
        <property type="interactions" value="1195"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29J99"/>
    <dbReference type="GeneID" id="4815413"/>
    <dbReference type="KEGG" id="dpo:4815413"/>
    <dbReference type="eggNOG" id="KOG1602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_0_4_1"/>
    <dbReference type="InParanoid" id="Q29J99"/>
    <dbReference type="OMA" id="FDRRDLW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070614">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29J99">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000005">
        <property type="entry name" value="Alkyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001355345.1"/>
        </source>
    </evidence>
    <sequence length="303" mass="35224" checksum="A7DC88A97A00BC4B" modified="2006-04-04" version="1">MSWVADYEYTWAERISMRALRTCGHIPKHVAFVMDGNRRFARSQQIDKIEGHSRGFSKLADCLRWCLDLGINEVTTFAFSIENFKRSSEEVEGLFNLARDKFAKLLKETDRLNQHGIRIRVIGNIGLLPLDLQELVATAMLSTEVNDKLFLNVAFSYTSRDEITQALETVLQQGNSFISGSNISERLLEECMYTRHSPAPDLIFRTSGETRLSDFMMWQLSGSVLYFSNVLWPQITFWNFLASILAYQRDRYRLEKFRRAEQIQSAQLARTSDFHSERVQQFLQSMDEDRRKLLVRLASSKMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LL7</accession>
    <accession>A0A6I8UKL9</accession>
    <name>Q29LL7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ovomucoid-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4817555</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356962.1">
        <property type="nucleotide sequence ID" value="XM_001356926.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LL7"/>
    <dbReference type="SMR" id="Q29LL7"/>
    <dbReference type="FunCoup" id="Q29LL7">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="GeneID" id="4817555"/>
    <dbReference type="KEGG" id="dpo:4817555"/>
    <dbReference type="eggNOG" id="ENOG502T8NR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169765_5_1_1"/>
    <dbReference type="InParanoid" id="Q29LL7"/>
    <dbReference type="OMA" id="QCDLNCA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076423">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LL7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF45">
        <property type="entry name" value="FOLLISTATIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00282">
        <property type="entry name" value="KAZAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001356962.1"/>
        </source>
    </evidence>
    <sequence length="71" mass="7896" checksum="493D61C78697FDD1" modified="2006-04-04" version="1">MRFFALIALCLFALLSLTVAQEADFCPCPRNFEPVCGSDSRTYSNKCDLECQATKASRQGRSITLIKEGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZQ8</accession>
    <accession>A0A6I8UDL3</accession>
    <name>Q2LZQ8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Phosphatidylinositol-4,5-bisphosphate 4-phosphatase</fullName>
            <ecNumber evidence="4 11">3.1.3.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16 17" type="primary">LOC4813276</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15 16">MV-25-SWS-2005</strain>
            <tissue evidence="14 15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25674"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Late endosome membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 11">Lysosome membrane</location>
            <topology evidence="3 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.78" evidence="4 11"/>
    <dbReference type="RefSeq" id="XP_001353715.1">
        <property type="nucleotide sequence ID" value="XM_001353679.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043082.1">
        <property type="nucleotide sequence ID" value="XM_015187596.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043083.1">
        <property type="nucleotide sequence ID" value="XM_015187597.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043084.1">
        <property type="nucleotide sequence ID" value="XM_015187598.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZQ8"/>
    <dbReference type="STRING" id="46245.Q2LZQ8"/>
    <dbReference type="GeneID" id="4813276"/>
    <dbReference type="KEGG" id="dpo:4813276"/>
    <dbReference type="eggNOG" id="KOG4684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087485_1_0_1"/>
    <dbReference type="OMA" id="WLRIKIS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079796">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZQ8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034597">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019178">
        <property type="entry name" value="PtdIns-P2-Ptase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21014">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21014:SF6">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09788">
        <property type="entry name" value="Tmemb_55A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0967">Endosome</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001353715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015043082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015043083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015043084.1"/>
        </source>
    </evidence>
    <sequence length="266" mass="28524" checksum="090E0F214FD5BED0" modified="2006-02-21" version="1">MADPERQSLINKDESIMYTSSAAVDEGAENPGSSTAAVPNEDGNGAPTAVPCIGPDELPPPYQQSSNTGGVPMVTCRVCQHMIDITTKREQHVVKCTHCNEATPIRNAPPGKKYVRCPCNCLLICKSSSQRIACPRPNCKRIINLAPSPVTPPVPTMPGMCRVICGHCSDTFLFNTYHNALARCPHCRKVSSVGSRFANSRGVMFAIVALLFLVAGIAVTIGTISYASAHGGMYILYVALFVIAVSMLARSAYYFRLKVSAIDGPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M110</accession>
    <accession>A0A6I8UCF2</accession>
    <name>Q2M110_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrator complex subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IntS9</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS9 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353263.1">
        <property type="nucleotide sequence ID" value="XM_001353227.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M110"/>
    <dbReference type="FunCoup" id="Q2M110">
        <property type="interactions" value="1831"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M110"/>
    <dbReference type="GeneID" id="4813543"/>
    <dbReference type="KEGG" id="dpo:4813543"/>
    <dbReference type="eggNOG" id="KOG1138">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023159_1_0_1"/>
    <dbReference type="InParanoid" id="Q2M110"/>
    <dbReference type="OMA" id="AMKAVHC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078747">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M110">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160232">
        <property type="term" value="C:INTAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090101">
        <property type="term" value="P:negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090287">
        <property type="term" value="P:regulation of cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160240">
        <property type="term" value="P:RNA polymerase II transcription initiation surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000003">
        <property type="entry name" value="Integrator complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000056">
        <property type="entry name" value="integrator complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027074">
        <property type="entry name" value="Integrator_9su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048660">
        <property type="entry name" value="IntS9-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094:SF1">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21382">
        <property type="entry name" value="IntS9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-Casp" evidence="7">
        <location>
            <begin position="304"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353263.1"/>
        </source>
    </evidence>
    <sequence length="657" mass="73861" checksum="B5E0710B6BE24810" modified="2006-02-21" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M180</accession>
    <accession>A0A6I8UCA9</accession>
    <name>Q2M180_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enhancer of split malpha protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Tom</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353192.1">
        <property type="nucleotide sequence ID" value="XM_001353156.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M180"/>
    <dbReference type="FunCoup" id="Q2M180">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M180"/>
    <dbReference type="GeneID" id="4813363"/>
    <dbReference type="KEGG" id="dpo:4813363"/>
    <dbReference type="eggNOG" id="ENOG502SA2K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106880_0_0_1"/>
    <dbReference type="InParanoid" id="Q2M180"/>
    <dbReference type="OMA" id="ADRDLCY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078719">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029686">
        <property type="entry name" value="Malpha/m4/m2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12254:SF0">
        <property type="entry name" value="BARBU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12254">
        <property type="entry name" value="ENHANCER OF SPLIT MALPHA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15952">
        <property type="entry name" value="ESM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353192.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="18377" checksum="928B0BA3C0F45B5D" modified="2006-02-21" version="1">MSFITREYKFESSMVQQKSDQHTMAMRSLKKLVKPLLRLVKKKQLLRKTLAEIQNQNDINASLEDMRKDPAVSCDNMANEQLEQRLYNDLKQCPSNVAMIVQQGQQQHVVPVQPEQTFIPVHFARTSSGTFFWTTAEGARQHQEQQLRQQFDRWVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P972</accession>
    <accession>A0A6I8VMM4</accession>
    <name>A0A0R3P972_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neural/ectodermal development factor IMP-L2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ImpL2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015044106.1">
        <property type="nucleotide sequence ID" value="XM_015188620.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P972"/>
    <dbReference type="SMR" id="A0A0R3P972"/>
    <dbReference type="FunCoup" id="A0A0R3P972">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P972"/>
    <dbReference type="GeneID" id="4812663"/>
    <dbReference type="InParanoid" id="A0A0R3P972"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076803">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001749">
        <property type="entry name" value="Neural/ectodermal development factor IMP-L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF253">
        <property type="entry name" value="DPR-INTERACTING PROTEIN ETA, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="48"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="178"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015044106.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="30307" checksum="2EE49CF15160CB2A" modified="2016-01-20" version="1">MQKMNLSLCALALLLFGSLSSVQSRAVDIVDDSNDVDNSIEAEPEEKPRGRPFEADWLKFTKSPPAKLQQAAGATLEIVCEMMGSQVPSIQWVVGHMPLSEIENLESNVVSEDAASAIVRVRSVHIIDHMLSEPRTYTCVGRTGSKTVYASTVVHPVRSDLVDMRMVREKQYPGPQKPRIIYTEKTHLDLIGSSVMLPCKVHARPRAEVTWLNNENKEVVQSHRYKVLPTGDLLISEIKWEDMGNYKCIARNSAGRDSADTFVYPVLKEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAU7</accession>
    <name>A0A6I8UAU7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sensory neuron membrane protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Snmp2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352528.4">
        <property type="nucleotide sequence ID" value="XM_001352492.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAU7"/>
    <dbReference type="FunCoup" id="A0A6I8UAU7">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812316"/>
    <dbReference type="InParanoid" id="A0A6I8UAU7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UAU7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF109">
        <property type="entry name" value="SENSORY NEURON MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001352528.4"/>
        </source>
    </evidence>
    <sequence length="580" mass="65221" checksum="3CF7233F1CF2D8CD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCD7</accession>
    <name>A0A6I8UCD7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Cytochrome P450 4d8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Cyp4d8</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353245.3">
        <property type="nucleotide sequence ID" value="XM_001353209.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCD7"/>
    <dbReference type="FunCoup" id="A0A6I8UCD7">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813591"/>
    <dbReference type="InParanoid" id="A0A6I8UCD7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCD7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20628">
        <property type="entry name" value="CYP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF187">
        <property type="entry name" value="CYTOCHROME P450 4AE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026194605" evidence="15">
        <location>
            <begin position="23"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001353245.3"/>
        </source>
    </evidence>
    <sequence length="507" mass="58187" checksum="70C5E153ED8EDF77" modified="2020-08-12" version="1">MLLLVLVLLLVAVGWLVHRGQAERRRAVSNLPGPMCPPLLGALHIMSSMNPKTFIKVSGENMRKYGPLQRVWILNRLLIMSADSEINEQLLSSQEHLVKHPVYRVLGKWLGNGLLLSDGKVWHQRRKIITPTFHFSILEQFVEVFDQQSNVCVERLAPRANGQTFDVYRPVCMAALDIIAETAMGTKIHAQAAESTPYAEAVNECTALLSWRFISVYLQVELLFTLTHPYMKWRQTQLIRTMHDFTTKVIEERRRTLEEQQGSKLSPSPDDDVGRKRRMALLDVLLLATVDGRPLTNDEIREEVDTFMFEGHDTTTSALSFCLHVLARHPQVQEKMLEEILRVIGTDRKRPVTIRDLNEMKYLECVIKESLRLYPPVPLVGRKLQTDFKYSHSKYGDGVIPAGSEFLIGIYGMQRNKNEFPQSEEFIPERHENGERFSPFTFIPFSAGPRNCIGQKFAQLEMKMMLAKIVREYELLPLGEPVECVINIVLRSDTGFQLGMRRRNDTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCM0</accession>
    <name>A0A6I8UCM0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein inturned</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">in</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353327.4">
        <property type="nucleotide sequence ID" value="XM_001353291.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCM0"/>
    <dbReference type="FunCoup" id="A0A6I8UCM0">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813426"/>
    <dbReference type="InParanoid" id="A0A6I8UCM0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001736">
        <property type="term" value="P:establishment of planar polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043988">
        <property type="entry name" value="CCZ1/INTU_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039151">
        <property type="entry name" value="INTU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21082">
        <property type="entry name" value="PROTEIN INTURNED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21082:SF4">
        <property type="entry name" value="PROTEIN INTURNED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19032">
        <property type="entry name" value="Intu_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="5">
        <location>
            <begin position="305"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU second Longin" evidence="6">
        <location>
            <begin position="506"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HPS4 third Longin" evidence="7">
        <location>
            <begin position="767"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="686"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353327.4"/>
        </source>
    </evidence>
    <sequence length="905" mass="101094" checksum="4B4EBD6955E4A81A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCZ8</accession>
    <name>A0A6I8UCZ8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cullin homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Cul6</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the cullin family.</text>
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    <dbReference type="RefSeq" id="XP_001353499.4">
        <property type="nucleotide sequence ID" value="XM_001353463.4"/>
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    <dbReference type="KEGG" id="dpo:4813684"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
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        <property type="entry name" value="Cullin_homology"/>
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        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="341"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001353499.4"/>
        </source>
    </evidence>
    <sequence length="693" mass="81030" checksum="0E62C25DBEA9DB85" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDN2</accession>
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein Ir67b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ir67b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353772.4">
        <property type="nucleotide sequence ID" value="XM_001353736.4"/>
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        <property type="interactions" value="5"/>
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    <dbReference type="KEGG" id="dpo:4812891"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF39">
        <property type="entry name" value="IONOTROPIC RECEPTOR 56A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="547"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353772.4"/>
        </source>
    </evidence>
    <sequence length="576" mass="65573" checksum="12BA6C52DAF72A78" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG88</accession>
    <name>A0A6I8UG88_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4814979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354937.2">
        <property type="nucleotide sequence ID" value="XM_001354901.4"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8UG88"/>
    <dbReference type="FunCoup" id="A0A6I8UG88">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814979"/>
    <dbReference type="InParanoid" id="A0A6I8UG88"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UG88">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.820:FF:000006">
        <property type="entry name" value="Blast:BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000169">
        <property type="entry name" value="BTB/POZ domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.820">
        <property type="entry name" value="PHR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038648">
        <property type="entry name" value="PHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774:SF3">
        <property type="entry name" value="BTB (POZ) DOMAIN-CONTAINING 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="26"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="515"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354937.2"/>
        </source>
    </evidence>
    <sequence length="720" mass="79087" checksum="140253D34B5DD4F0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIV6</accession>
    <name>A0A6I8UIV6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4816757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356076.4">
        <property type="nucleotide sequence ID" value="XM_001356040.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIV6"/>
    <dbReference type="KEGG" id="dpo:4816757"/>
    <dbReference type="InParanoid" id="A0A6I8UIV6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356076.4"/>
        </source>
    </evidence>
    <sequence length="306" mass="35545" checksum="15A2BED0718DD0BA" modified="2020-08-12" version="1">MLFMESYMCYCSVRLGVIIVAILTIIRCMALSIVLFLMGVKCFDPLIDLFEHDSDYKDRKSIRKLINWIENSPENCLVSLQVLLYAHTAAAILSIWGSLKLQKWLVLPLAFFELMYFLQFTIQHIILMIMLKKQINLGLLIILTLIGSFYILFVGYNAITCVAMFQVISLVRSPCYRELYGDDPFHPLATKSHPHKDSSVQQLRVLKMDNDNDMDIDEQKRLSKLGLWPRRYPQVVSVIPVQAPYPALKWWQLQAMDTGQDKLQLDSSAVYRNWQQGELLNGVGGEVQRKNALNRRYAESQMHRWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJ59</accession>
    <name>A0A6I8UJ59_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein axonemal assembly factor 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Defective transmitter-recycling protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Dynein regulatory complex subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Leucine-rich repeat-containing protein 50 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TbCMF46</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium-specific protein required for cilia structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the DRC3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356335.2">
        <property type="nucleotide sequence ID" value="XM_001356299.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJ59"/>
    <dbReference type="FunCoup" id="A0A6I8UJ59">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817313"/>
    <dbReference type="InParanoid" id="A0A6I8UJ59"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF12">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Rho-GAP" evidence="17">
        <location>
            <begin position="454"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="323"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001356335.2"/>
        </source>
    </evidence>
    <sequence length="555" mass="64836" checksum="0B61B65FBE296C2A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJG3</accession>
    <name>A0A6I8UJG3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc carboxypeptidase A 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4817350</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356357.2">
        <property type="nucleotide sequence ID" value="XM_001356321.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJG3"/>
    <dbReference type="FunCoup" id="A0A6I8UJG3">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817350"/>
    <dbReference type="InParanoid" id="A0A6I8UJG3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF156">
        <property type="entry name" value="RH39904P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026163961" evidence="12">
        <location>
            <begin position="19"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="118"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001356357.2"/>
        </source>
    </evidence>
    <sequence length="426" mass="48235" checksum="159CA4ADE41FC9C6" modified="2020-08-12" version="1">MKVLLVSLSLSLLALCLCERLTFEDHRVYRLEPKNAEQLEGLRPLEATNERVIFLNDLHLGAINALIAPSYNEEFLQLLQSLDISPELLDANAQASLSLDIEPVADANRRSDDYSWTEYHELNDTHRWMQNLVEKNPGIVSVFVAGRSYEGRDLLGLRIFHSAGAERGEQQAIFLEAGMHAREWISPATATYFANQLLTSQQPEIQSLARSYVWYILPHANPDGYVYTHSTNRLWRKTRSPQEKNCMGTDPNRNWGFHWNEVGASDEPCSESYAGPKAFSEPETEGLSQYLKSLPEPVFMYLSLHSFSQLLLYPYGHTSSLPENHRQLERIFSAALGGMKRRFGTRYTGGNVYDAIYPAAGSSMDWAYGELKTKYSFTYELRPSGYSFWTGFRLPASQIIPTGQETTDSVVEMVKAAASIEGYKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJQ9</accession>
    <name>A0A6I8UJQ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sodium-coupled monocarboxylate transporter 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4817241</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356615.4">
        <property type="nucleotide sequence ID" value="XM_001356579.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJQ9"/>
    <dbReference type="FunCoup" id="A0A6I8UJQ9">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817241"/>
    <dbReference type="InParanoid" id="A0A6I8UJQ9"/>
    <dbReference type="OMA" id="VTKPTNM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081076">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJQ9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11492">
        <property type="entry name" value="SLC5sbd_NIS-SMVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF41">
        <property type="entry name" value="GH19970P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001356615.4"/>
        </source>
    </evidence>
    <sequence length="603" mass="65790" checksum="6D30DB278BB38440" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK68</accession>
    <name>A0A6I8UK68_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">U4/U6.U5 tri-snRNP-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4817013</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNU66/SART1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356840.3">
        <property type="nucleotide sequence ID" value="XM_001356804.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK68"/>
    <dbReference type="FunCoup" id="A0A6I8UK68">
        <property type="interactions" value="2058"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817013"/>
    <dbReference type="InParanoid" id="A0A6I8UK68"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079776">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000481">
        <property type="term" value="P:maturation of 5S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005011">
        <property type="entry name" value="SNU66/SART1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152">
        <property type="entry name" value="SQUAMOUS CELL CARCINOMA ANTIGEN RECOGNISED BY CYTOTOXIC T LYMPHOCYTES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152:SF5">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03343">
        <property type="entry name" value="SART-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="575"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="174"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356840.3"/>
        </source>
    </evidence>
    <sequence length="935" mass="108025" checksum="221A87620857129D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKG5</accession>
    <name>A0A6I8UKG5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein chiffon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">chif</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356906.4">
        <property type="nucleotide sequence ID" value="XM_001356870.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UKG5">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817485"/>
    <dbReference type="InParanoid" id="A0A6I8UKG5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010571">
        <property type="term" value="P:positive regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3410:FF:000001">
        <property type="entry name" value="Protein DBF4 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3410">
        <property type="entry name" value="DBF zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051590">
        <property type="entry name" value="Replication_Regulatory_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006572">
        <property type="entry name" value="Znf_DBF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038545">
        <property type="entry name" value="Znf_DBF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375">
        <property type="entry name" value="ACTIVATOR OF S-PHASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375:SF26">
        <property type="entry name" value="PROTEIN CHIFFON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07535">
        <property type="entry name" value="zf-DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00586">
        <property type="entry name" value="ZnF_DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51265">
        <property type="entry name" value="ZF_DBF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DBF4-type" evidence="6">
        <location>
            <begin position="337"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="83"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="269"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="413"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="541"/>
            <end position="563"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="584"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="779"/>
            <end position="905"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="987"/>
            <end position="1022"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1127"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1196"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1267"/>
            <end position="1288"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1343"/>
            <end position="1364"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1379"/>
            <end position="1408"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1426"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1469"/>
            <end position="1740"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="10"/>
            <end position="26"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="106"/>
            <end position="117"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="271"/>
            <end position="280"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="289"/>
            <end position="298"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="338"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="413"/>
            <end position="437"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="462"/>
            <end position="475"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="479"/>
            <end position="495"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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            <end position="507"/>
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            <end position="604"/>
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            <begin position="609"/>
            <end position="620"/>
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            <begin position="623"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="655"/>
            <end position="668"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
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        <location>
            <begin position="730"/>
            <end position="753"/>
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            <begin position="780"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="1664"/>
            <end position="1691"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00600"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51265"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356906.4"/>
        </source>
    </evidence>
    <sequence length="1798" mass="198443" checksum="51B6C2C9BFAABF21" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKY4</accession>
    <name>A0A6I8UKY4_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Aspartic proteinase A3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4817636</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001356995.4"/>
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    <dbReference type="InParanoid" id="A0A6I8UKY4"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Lysosomal aspartic protease"/>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Aspartic_peptidase_A1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026313632" evidence="7">
        <location>
            <begin position="20"/>
            <end position="387"/>
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    </feature>
    <feature type="domain" description="Peptidase A1" evidence="8">
        <location>
            <begin position="84"/>
            <end position="384"/>
        </location>
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    <feature type="active site" evidence="5">
        <location>
            <position position="100"/>
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    <feature type="active site" evidence="5">
        <location>
            <position position="278"/>
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    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00007447"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357031.4"/>
        </source>
    </evidence>
    <sequence length="387" mass="42563" checksum="1632BC88527FC6E3" modified="2020-08-12" version="1">MNQRVVILLLCAFLCASEAFHRIAMQRHRHRKLPHGRHLWASRKHMRRKFGMPAVTTQSTANTSTTSAPSEAIEPLFNAFQTQFYGPLFVSDQAFTVQFDTGSSDLWIPNSNCSTCIKECGNAVFQMAKSKSFKANGTSFSITYGVGKVSGVTASETVSIGQISIKDQGFGLVSQTDTCSSFDGVLGMAYPGASVTGARPPFYQMIDQKLVDSSIFSFHLKSGDGGSLILGGSNSSLYHGSLTHVNVTQQKMWQFTMDYIGFPSKKCRRCNSCKAIMDSGTSLIVGPTEDITQLNTKIRATYNATNGIWQVPCGRISYLPVLELGIAGKKFQVRPQEYVIAYEGDVCITGFMDLAGLNFWIIGDVFFREHYVSFDVELNRIGIAKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL48</accession>
    <name>A0A6I8UL48_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Solute carrier organic anion transporter family member 3A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">Oatp30B</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357243.4">
        <property type="nucleotide sequence ID" value="XM_001357207.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035302.2">
        <property type="nucleotide sequence ID" value="XM_015179816.2"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
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            <begin position="195"/>
            <end position="215"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="235"/>
            <end position="255"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <end position="280"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="347"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="384"/>
            <end position="411"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="431"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="548"/>
            <end position="567"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="587"/>
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            <begin position="620"/>
            <end position="640"/>
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            <begin position="767"/>
            <end position="793"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="805"/>
            <end position="828"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="858"/>
            <end position="880"/>
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        <location>
            <begin position="669"/>
            <end position="718"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="992"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1120"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1200"/>
            <end position="1257"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
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        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
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            <begin position="93"/>
            <end position="109"/>
        </location>
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        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
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        <location>
            <begin position="1000"/>
            <end position="1013"/>
        </location>
    </feature>
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        <location>
            <begin position="1150"/>
            <end position="1170"/>
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            <begin position="1220"/>
            <end position="1231"/>
        </location>
    </feature>
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        <location>
            <begin position="1232"/>
            <end position="1241"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
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        <source>
            <dbReference type="RefSeq" id="XP_001357243.4"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015035302.2"/>
        </source>
    </evidence>
    <sequence length="1257" mass="135185" checksum="FE4449092CA3B177" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM68</accession>
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        <submittedName>
            <fullName evidence="7">Nucleoplasmin-like protein</fullName>
        </submittedName>
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        <name evidence="7" type="primary">Nph</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="7">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <text evidence="2">Belongs to the nucleoplasmin family.</text>
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            <begin position="6"/>
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            <begin position="122"/>
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            <begin position="146"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010744"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001357629.2"/>
        </source>
    </evidence>
    <sequence length="157" mass="17375" checksum="A0331EC6281C13EB" modified="2020-08-12" version="1">MESESFYGVTLSEKDPIAQFDVPDIPEEYVVHSHKLIIKQISLGSEAKTGEFNVVQAETNVNDDGEKKTVKIPIAVLKVGETRSLRPNVEFPNGSVTFKLVQGTGPVYVCGKAEMNFGEFDDGQMYEEYSDDEEDSDGDFDEDAVPQTNGKSKITKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UME3</accession>
    <name>A0A6I8UME3_DROPS</name>
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        <submittedName>
            <fullName evidence="14">Tyrosine-protein kinase Wsck</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Wsck</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357726.2">
        <property type="nucleotide sequence ID" value="XM_001357689.4"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8UME3"/>
    <dbReference type="GeneID" id="4800453"/>
    <dbReference type="KEGG" id="dpo:4800453"/>
    <dbReference type="InParanoid" id="A0A6I8UME3"/>
    <dbReference type="OMA" id="WIYELAS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076045">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF617">
        <property type="entry name" value="RET ONCOGENE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23144">
        <property type="entry name" value="Fn3_PTPRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026114447" evidence="9">
        <location>
            <begin position="39"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="12">
        <location>
            <begin position="45"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="137"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="500"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001357726.2"/>
        </source>
    </evidence>
    <sequence length="799" mass="89086" checksum="9BF9016A45ABADA9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMT5</accession>
    <name>A0A6I8UMT5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">HSPB1-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">HSPBAP1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May play a role in cellular stress response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357902.3">
        <property type="nucleotide sequence ID" value="XM_001357865.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMT5"/>
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        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800670"/>
    <dbReference type="InParanoid" id="A0A6I8UMT5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF43">
        <property type="entry name" value="HSPB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="4">
        <location>
            <begin position="94"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001357902.3"/>
        </source>
    </evidence>
    <sequence length="398" mass="46194" checksum="4D21BF175AA1A988" modified="2020-08-12" version="1">MEALSSQLRDLILNTRVPLIIDQFPIGWECFRGSLHDWCERFDKEAAGPPAFELMSLTDCSTPQWERKRMRVNDMSMQQFLRDYGYLEDDQTHWAAYQYKRAHEVPASCLKGIDFSCFGFPEHQNDFTFWLGSEQANTPCHYDTFGVNIVVQVYGSKSWLLFPPETPLQSTRVPYEESSVYCLDNFYAPAQDKLPRLLEYSDQAYQCTLQPGDVLIVPRHWWHYVEATATSLSLNYWVPLKDDMDLALDEFLVKHIVESFVKGESEQTKQYLLNPNQLEDVSSTPSELFAQFQQAVQNAESEEHKRKLWETDYLTQSKFRELLARVLLTVRPLEVMPKEEYKLLLESNSKRLQAETRTAAASSPISSTLELLISSMCAPRTIAGMKREFFRRLRQGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN17</accession>
    <name>A0A6I8UN17_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein lmd</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lmd</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358101.4">
        <property type="nucleotide sequence ID" value="XM_001358064.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN17"/>
    <dbReference type="FunCoup" id="A0A6I8UN17">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800912"/>
    <dbReference type="InParanoid" id="A0A6I8UN17"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000019">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000031">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000048">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000359">
        <property type="entry name" value="GLIS family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000453">
        <property type="entry name" value="GLIS family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF7">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="518"/>
            <end position="547"/>
        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001358101.4"/>
        </source>
    </evidence>
    <sequence length="917" mass="98897" checksum="ACB2C5674BC08B5D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP34</accession>
    <name>A0A6I8UP34_DROPS</name>
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        <recommendedName>
            <fullName evidence="13">Protein glass</fullName>
        </recommendedName>
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    <gene>
        <name evidence="18" type="primary">gl</name>
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    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
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    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <text evidence="12">Transcription factor required for gene expression specific to photoreceptor cells.</text>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:visual perception"/>
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        <location>
            <begin position="225"/>
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        <location>
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            <end position="592"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00058744"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00067600"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001358547.4"/>
        </source>
    </evidence>
    <sequence length="592" mass="61300" checksum="53822EBFFE1ACF55" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP62</accession>
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            <fullName evidence="12">Lachesin</fullName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
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        </source>
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        <subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="entry name" value="IGc2"/>
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    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
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    </feature>
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        <location>
            <begin position="40"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="142"/>
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        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="243"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358675.3"/>
        </source>
    </evidence>
    <sequence length="415" mass="45563" checksum="715E3A567ACC7571" modified="2020-08-12" version="1">MEKLLLSPALFVILIMATKCGNAVAQNQHHESNSQLDPDPEFIGFINNVTYPAGREAILACSVRNLGKNKVGWLRASDQTVLALQGRVVTHNARISVMHQDMHTWKLKISKLRESDRGCYMCQINTSPMKKQVGCIDVQVPPDIINEDSSADMAVQEGEDATLTCKATGNPLPRVIWRREDGEMILIRKPGSRELMKVESYNGSSLRLLRLERRQMGAYLCIASNDVPPAVSKRVSLSVQFAPMVRAPSQLLGTPLGSDVQLECQVEASPSPVSYWLKGARTSNGFASVSTASLESGSPGPEMLLDGPKYGITERRDGYRGVMLLVVRSFSPSDVGTYHCVSTNSLGRAEGTLRLYEIKLHPGAAASNDDHLNFVGGLEDAARSVATRRHTTWQPLWMLMLLLLLMPLRQRPGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPC7</accession>
    <name>A0A6I8UPC7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Organic cation transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Orct</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably transports organic cations.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358762.3">
        <property type="nucleotide sequence ID" value="XM_001358725.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPC7"/>
    <dbReference type="FunCoup" id="A0A6I8UPC7">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801717"/>
    <dbReference type="InParanoid" id="A0A6I8UPC7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17317">
        <property type="entry name" value="MFS_SLC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000553">
        <property type="entry name" value="Organic cation transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="87"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001358762.3"/>
        </source>
    </evidence>
    <sequence length="556" mass="61501" checksum="78A040E33D79A8A3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPW8</accession>
    <name>A0A6I8UPW8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transferase CAF17 homolog, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4801970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358983.2">
        <property type="nucleotide sequence ID" value="XM_001358946.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPW8"/>
    <dbReference type="FunCoup" id="A0A6I8UPW8">
        <property type="interactions" value="728"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801970"/>
    <dbReference type="InParanoid" id="A0A6I8UPW8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057460">
        <property type="entry name" value="CAF17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GCV_T_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602:SF0">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25455">
        <property type="entry name" value="Beta-barrel_CAF17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CAF17 C-terminal" evidence="4">
        <location>
            <begin position="258"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001358983.2"/>
        </source>
    </evidence>
    <sequence length="340" mass="38949" checksum="1691380F8CBF059B" modified="2020-08-12" version="1">MNICKNFKFLRLTRCCMRNFADIRPEQKSTYTLEKLAHRELIRVHGPEVLPFLQGLVTNDVSHLQRPDGPSSIYALFLNKGGRVLYDTIVYRTESPDTYLLECDREASEEFRRHLRTFRVRRKIDVDSIDDEYAPWVLFNTQKCGEQVGEKLQRNKERKWFISSDPRLPTLGTRILAPTDFNGLLRAKEMIVTPPSSENNYQLLRYKQGVGEGSEELPPGKCFPLEANADFLNGVSFNKGCYVGQELTARIHHSGVIRKRYMPIRLTAPLGANQTVQSLAGANLGRVFGHAHNHGVALLRIEQVLNGHQELMVDGERCFAERPDWWPSDLPSKRRQALTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ62</accession>
    <name>A0A6I8UQ62_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphotransferase</fullName>
            <ecNumber evidence="12">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Hex-t1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_001359146.2">
        <property type="nucleotide sequence ID" value="XM_001359109.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ62"/>
    <dbReference type="FunCoup" id="A0A6I8UQ62">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="GeneID" id="4802174"/>
    <dbReference type="KEGG" id="dpo:4802174"/>
    <dbReference type="InParanoid" id="A0A6I8UQ62"/>
    <dbReference type="OMA" id="VYMSSKC"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077295">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="13">
        <location>
            <begin position="15"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="14">
        <location>
            <begin position="216"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001359146.2"/>
        </source>
    </evidence>
    <sequence length="466" mass="52304" checksum="240574A1D7B972FD" modified="2020-08-12" version="1">MAGPIFKPEVDFPEVYKLCQMFVPSENDLVKVRDTMNREMTAGLSKEGHDRSSVPCYLSYVQDLPSGRERGRFLALEMMPTNCRIMLVRFSSEKDVYMSSKCVIIPHSIAAAKGTELFNFLASNIATFVKDKKVEKENLPMGIAFAFSLNKLALDVGILIAWTKEFGATGAIGKDVVQLLRDALAKFPEIMVNILGIINVAIGSLLALCWTQPDTRIGLNMGNITNSCYVEEVDNCHMYTGTNNKSIMIVNTDWAHFGDGGQLDFIRNEFDKTLDESSTNPGHRIYEKFSGALFISELVRMVIVRLMRNGTIFEGQTLDLISNQWRMDMSSVFDLDSDPPGVYTKAQEVMEKYRIRHCKERDLAAVRYISNIILSRAAMMLSSGVACLINRMKIAQISISVDGGIYRLNQTFQTVINKYTLQLTDPRYKFEYVVNQDSCGVGAAIMAGLAHADKYRGESKIFELDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ78</accession>
    <name>A0A6I8UQ78_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Enhancer of split mgamma protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">E(spl)mgamma-HLH</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359174.1">
        <property type="nucleotide sequence ID" value="XM_001359137.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ78"/>
    <dbReference type="FunCoup" id="A0A6I8UQ78">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="GeneID" id="4802207"/>
    <dbReference type="KEGG" id="dpo:4802207"/>
    <dbReference type="InParanoid" id="A0A6I8UQ78"/>
    <dbReference type="OMA" id="NEPIWRP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080985">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19741">
        <property type="entry name" value="bHLH-O_ESMB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000009">
        <property type="entry name" value="Transcription factor HES-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="15"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="8">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359174.1"/>
        </source>
    </evidence>
    <sequence length="214" mass="23848" checksum="13918C5343E56EB6" modified="2020-08-12" version="1">MSSSQISEMSKTYQYRKVMKPMLERKRRARINKCLDELKDLMVATLESEGEHVTRLEKADILELTVTHLQKMKEKRQHKRATGDESLSPAEGFRSGYIHAVNEVSRSLSHLPGMNVSLGTQLMTHLGQRLNQLQPAVKEALPITAPLSVHIASRDAYSVPISPVSSFAGSPNSSLASSERLGSASLLTTCSSIDVTKMELEVDSEDEENVWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQQ2</accession>
    <name>A0A6I8UQQ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 22 member 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4802527</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359431.4">
        <property type="nucleotide sequence ID" value="XM_001359394.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQQ2"/>
    <dbReference type="KEGG" id="dpo:4802527"/>
    <dbReference type="InParanoid" id="A0A6I8UQQ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UQQ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17317">
        <property type="entry name" value="MFS_SLC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="612"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="641"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="673"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="716"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359431.4"/>
        </source>
    </evidence>
    <sequence length="840" mass="93152" checksum="3B72BE71C19D3EDF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQR4</accession>
    <name>A0A6I8UQR4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="3">4.2.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Hydroxymethylbilane hydrolyase [cyclizing]</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Uroporphyrinogen-III cosynthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Uros2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>hydroxymethylbilane = uroporphyrinogen III + H2O</text>
            <dbReference type="Rhea" id="RHEA:18965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="EC" id="4.2.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uroporphyrinogen-III synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.75" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001359442.3">
        <property type="nucleotide sequence ID" value="XM_001359405.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQR4"/>
    <dbReference type="FunCoup" id="A0A6I8UQR4">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802543"/>
    <dbReference type="InParanoid" id="A0A6I8UQR4"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006785">
        <property type="term" value="P:heme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10090:FF:000003">
        <property type="entry name" value="uroporphyrinogen-III synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390">
        <property type="entry name" value="UROPORPHYRINOGEN III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12390:SF0">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001359442.3"/>
        </source>
    </evidence>
    <sequence length="254" mass="28262" checksum="8127EFD792EFED3B" modified="2020-08-12" version="1">MANRQKRIVILLKGHTADYDRSQAMRQSNLSPIYIPPSQIVMKNLGLLKAKLEAPHRYAGIIFPGPRSVQAVYEALGNNGAMPNCWRALHNYALGASTHSRAYMTLRKFPTLGDRVVSAHNLCDLIVETIGPKRDLPLLLPYGNEAGNTLRIRLMAQGFRVDSCEVYENQIHPNFTNDMKKVLKAKQLETIVFHSLASVRSASEFFSKYKVSVNDRKLIAVGAAAQKAMEEAKLPVSAVVNSYSVDQLIKAIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQY6</accession>
    <name>A0A6I8UQY6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha-taxilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4802651</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the taxilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359532.2">
        <property type="nucleotide sequence ID" value="XM_001359495.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQY6"/>
    <dbReference type="FunCoup" id="A0A6I8UQY6">
        <property type="interactions" value="901"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802651"/>
    <dbReference type="InParanoid" id="A0A6I8UQY6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026183">
        <property type="entry name" value="Taxilin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127:SF13">
        <property type="entry name" value="GH01188P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127">
        <property type="entry name" value="TAXILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09728">
        <property type="entry name" value="Taxilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="440"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359532.2"/>
        </source>
    </evidence>
    <sequence length="540" mass="61355" checksum="A304AD0BF5798F17" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR47</accession>
    <name>A0A6I8UR47_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein downstream neighbor of son homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hd</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DONSON family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359393.2">
        <property type="nucleotide sequence ID" value="XM_001359356.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR47"/>
    <dbReference type="FunCoup" id="A0A6I8UR47">
        <property type="interactions" value="2001"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802478"/>
    <dbReference type="InParanoid" id="A0A6I8UR47"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024861">
        <property type="entry name" value="Donson"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972">
        <property type="entry name" value="DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972:SF0">
        <property type="entry name" value="PROTEIN DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02064">
        <property type="entry name" value="DONSON"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="309"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001359393.2"/>
        </source>
    </evidence>
    <sequence length="566" mass="62962" checksum="F5D39C3547040B48" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URJ4</accession>
    <name>A0A6I8URJ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">Kynurenine--oxoglutarate transaminase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">Kyat</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="10 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 12">MV-25-SWS-2005</strain>
            <strain evidence="9">MV2-25</strain>
            <tissue evidence="10 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid degradation; L-kynurenine degradation; kynurenate from L-kynurenine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359645.3">
        <property type="nucleotide sequence ID" value="XM_001359608.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038097.2">
        <property type="nucleotide sequence ID" value="XM_015182611.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038098.2">
        <property type="nucleotide sequence ID" value="XM_015182612.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URJ4"/>
    <dbReference type="KEGG" id="dpo:4802800"/>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00726"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000024">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000021">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="71"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359645.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015038097.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015038098.2"/>
        </source>
    </evidence>
    <sequence length="459" mass="51871" checksum="600492A3D01B76D2" modified="2020-08-12" version="1">MLRNCRSALQPIRLLLTAKDPCQPQPQRQQQSAAAAAASGSMEKFDLPKRLQGSTPSVWNEYIALAMQYKPLNLGQGFPDDAAPEYVTHSLADIAQDENPLLHQYTRGYGHVRLVQALSKLYSGLVGRELNPLSDILITSGAYEALYSTIMGHVDVGDEVIIIEPFFDCYEPMVKMAGGVSRFIPLKLRKTEQGPISSADWVLDDAELESLFNAKTKMIILNTPHNPIGKVFNRRELERIAELCRKWNVLCVSDEVYEWLVFDGAEHIRICTLPGMWDRTITLGSAGKTFSVTGWKIGWAYGPSQLIRNLQMVHQNSVYTCPTPLQEGVARSFEVELARLGTPESYFLSLPRELKEKRDFMAKFLSDAGMRPTIPEGGYFMLADWSPLASKVDLSSETDKHRDYKFTKWMTKNMGLQGIPPSAFYSEPNKPLGEDFVRYCFIKKQENLDKAAELLRKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URR9</accession>
    <name>A0A6I8URR9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="4">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4803083</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001359882.2">
        <property type="nucleotide sequence ID" value="XM_001359845.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URR9"/>
    <dbReference type="FunCoup" id="A0A6I8URR9">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803083"/>
    <dbReference type="InParanoid" id="A0A6I8URR9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000458">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF107">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="6">
        <location>
            <begin position="20"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="5">
        <location>
            <begin position="360"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359882.2"/>
        </source>
    </evidence>
    <sequence length="487" mass="56755" checksum="DAB8F26BFF433507" modified="2020-08-12" version="1">MENAKVENKTDIYHDSVVFITGATGFVGKTLLEKLLWSFPQIKRIYMLIRPKGGVSVKDRFQAFLQNRIFERLRAEHPERLKKIFHFAGNIEDDNFGLSEPDRARLCGEVNIIFHSAATVRFNECLKVAARVNSQATYNLLELCREMTQLRSFLYVSTAYCNPGRKYVDEEVYPTLPPVDWRQFLTCTKKVPDDYLNHLADYIKGPHVNTYTFTKSIAEQIVNAYKDVIPIVIVRPSIVTAAYREPYPGWIDNVQAISGIMMEIGKGGISSILGDKDMICDIIPVDFVVNAMIMMINKAKLGSLSICNATSGVTNPITWQHLGELTMKWSRIYPTKRMIMFPNFKYRRSASRHELAVWLLHFVPALLMDLRTLLLSQKKRMVTPIAKKFRQACLAGSFFSLNEWIFKNKSRFYFKEQIENGTFSMLYWNLEDLDYDDYVRRHMIGINKYLHRENFTVDSNKLMVTKVYWVWIFLHLCFYMLLIYVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US90</accession>
    <name>A0A6I8US90_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial import receptor subunit TOM40 homolog 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4803317</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360080.2">
        <property type="nucleotide sequence ID" value="XM_001360043.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US90"/>
    <dbReference type="GeneID" id="4803317"/>
    <dbReference type="KEGG" id="dpo:4803317"/>
    <dbReference type="InParanoid" id="A0A6I8US90"/>
    <dbReference type="OMA" id="DISYWQR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078237">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001360080.2"/>
        </source>
    </evidence>
    <sequence length="321" mass="36650" checksum="FC9763FA9D2F28D1" modified="2020-08-12" version="1">MSCKGCPPYEKDKSAIFFPRCFQWIRGACTKKNPGNVENLHGLSRNVMPKFFDGLELDYMHRLGPHKYITGSWILSHMKAAGFRFGGLYTFRLTNDVLLTPTIIGDIHPSTQAANLNILYYPYECLRLEATLQRSSEKTPVDSQYTIELTRPCDTWSANFYNVRKDNGRCTLSVMRAITQHWALGGEMLLEWNSPEKLSTDFALAARYSHHNFAVAASASRIGLDISYWQRIYPQIQMANLLAYNYITNKTIATVGYQWNFSDSAVHAMLDSDASVGFMWTKYLNNFPLQMGLSVIMSIPTDRFAFGMRFVVDPSGYRRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTT7</accession>
    <name>A0A6I8UTT7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 239</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4803882</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360530.1">
        <property type="nucleotide sequence ID" value="XM_001360493.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTT7"/>
    <dbReference type="FunCoup" id="A0A6I8UTT7">
        <property type="interactions" value="415"/>
    </dbReference>
    <dbReference type="GeneID" id="4803882"/>
    <dbReference type="KEGG" id="dpo:4803882"/>
    <dbReference type="InParanoid" id="A0A6I8UTT7"/>
    <dbReference type="OMA" id="KHETKCH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074525">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002578">
        <property type="entry name" value="Blast:Zinc finger protein 568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000646">
        <property type="entry name" value="Myeloid zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000925">
        <property type="entry name" value="Zinc finger protein 668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001360530.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="32324" checksum="988B3214EA1227DF" modified="2020-08-12" version="1">MAGIEVEFDETAVNQFSCKRCDRTFKSKRDQTLHRQEVHNHNKTTYECKLCAKSFCNSGNLDRHMKVHNDVRPFVCNICSKAFAQAVNLQRHFAVHSGERPFTCNFCNKSFTQQSNMKRHKMTHTGEKPFRCQRCGRYFSQLVNLKKHKLGHLNAKPYQCNYCEKGFTQLSNFKRHLQSHIKEGVDVDVPASIQAAAALARERLEAEQKPSFFECMVCRAIFDTFIDYEKHESKCNEDNERAALEVNQMAHMHPDDYLPMKFAVPDLDTHEIIIETTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUR4</accession>
    <name>A0A6I8UUR4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein PET100 homolog, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4804682</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PET100 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361203.1">
        <property type="nucleotide sequence ID" value="XM_001361166.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUR4"/>
    <dbReference type="FunCoup" id="A0A6I8UUR4">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="GeneID" id="4804682"/>
    <dbReference type="KEGG" id="dpo:4804682"/>
    <dbReference type="InParanoid" id="A0A6I8UUR4"/>
    <dbReference type="OMA" id="MALYMTF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073057">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018625">
        <property type="entry name" value="Pet100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968:SF1">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09803">
        <property type="entry name" value="Pet100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361203.1"/>
        </source>
    </evidence>
    <sequence length="80" mass="9829" checksum="5F027D00999155A9" modified="2020-08-12" version="1">MGTWVLEVAKMGMYMAFPVALFHIFNQPEYFEEWVTKKKRELYPPEHKSHREELQRAIREHHEKHDAKMMRAMEEAERKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV90</accession>
    <name>A0A6I8UV90_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein Strica</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Strica</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361329.2">
        <property type="nucleotide sequence ID" value="XM_001361292.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV90"/>
    <dbReference type="FunCoup" id="A0A6I8UV90">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804827"/>
    <dbReference type="InParanoid" id="A0A6I8UV90"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454:SF232">
        <property type="entry name" value="AT03047P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454">
        <property type="entry name" value="CASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Caspase family p20" evidence="5">
        <location>
            <begin position="301"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="4">
        <location>
            <begin position="435"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="104"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361329.2"/>
        </source>
    </evidence>
    <sequence length="511" mass="55707" checksum="0B3B43177528CD74" modified="2020-08-12" version="1">MGWWGKKRDDPETDRSSGSTNSQALVLQDPRTRVKTTSAATATTNTAVQNSTITDRNKQTITFLSTRQTVTHTQRALITETTTRRTPSQAELEQLFAQMRMGSDGATTTTTTTTTRGRPPSLNGVALRSTPSFKATTNNKRSSTVLKTEHTTVTKKNGRNVTQHLETQRVDLKTKGKPSWPASFFASTSSGGTSEPYVSPYAQKPSLAITYVSPYAKSTTSPTSTSSTSSVASAPSFGAFKPAAKPLFSTAVPRPSTGAIPKTSVGARDAQGIVKPKLFAGVGTTKPIVAKTKLKPARVYIFNNEKFDSKNEFRSGSAQDVKVLRTTFEQFKCKVEVIKDATLATVKSTVRMLETKDFEDKSALVLVFLSHGTRHDRLAAKDLDYSLDDDVLFPILRNRTLKDKPKMLFVQACKGANEPGGFMTDSAQPNGSPNEILKCYSTYEGYVSYRTEDGTPFIQTLCEALNRSGKTTDIDGIMMSVRSVVKMQTKDGQIPSVTSTLTSKYVFGDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW09</accession>
    <name>A0A6I8UW09_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peflin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Pef</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361750.2">
        <property type="nucleotide sequence ID" value="XM_001361713.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW09"/>
    <dbReference type="SMR" id="A0A6I8UW09"/>
    <dbReference type="FunCoup" id="A0A6I8UW09">
        <property type="interactions" value="1324"/>
    </dbReference>
    <dbReference type="GeneID" id="4805334"/>
    <dbReference type="KEGG" id="dpo:4805334"/>
    <dbReference type="InParanoid" id="A0A6I8UW09"/>
    <dbReference type="OMA" id="QFIVTCI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074682">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16184">
        <property type="entry name" value="EFh_PEF_peflin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361750.2"/>
        </source>
    </evidence>
    <sequence length="196" mass="22276" checksum="21F7B92109111B12" modified="2020-08-12" version="1">MSYGQGYNPYNQGYAAPPGAFPPQNSQVSSQAQQWFAMVDRDRSGKINASELQAALVNGRGDHFSDNACKLMISMFDNDASGTIDVYEFEKLYNYINQWLQVFKTYDQDSSGHIEESELTQAFTQMGFRFSPEFINFLVKKSDPQTHKEVSVDQFIVLCVQVQRFTEAFRQRDTQQNGTITIGFEDFLTVAIGCSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW53</accession>
    <name>A0A6I8UW53_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Camp isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Camp</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361804.2">
        <property type="nucleotide sequence ID" value="XM_001361767.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW53"/>
    <dbReference type="KEGG" id="dpo:4805399"/>
    <dbReference type="InParanoid" id="A0A6I8UW53"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UW53">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23080:SF144">
        <property type="entry name" value="SPINDLE AND KINETOCHORE ASSOCIATED COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23080">
        <property type="entry name" value="THAP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001361804.2"/>
        </source>
    </evidence>
    <sequence length="488" mass="53760" checksum="B37B06B22BE494BD" modified="2020-08-12" version="1">MFYIASNIRRNIKNEEYVKWNLSSSVYSRYILLEAGDGIVSATSDGIVLNKIPALIRTTMKKAATSTTSAAAFGTTPLKNPKYVIQAGSAAAGISPWHSVTAIKRESLGIAISSLPPNTIIKRATPAPKSSKSTLSSLLGSQPTPVAAVAPATRVSSAVHQAVFIKRELPQQRSMRAVTLGLMENATMLHLGLASEHLSILKRHICRSAAVTHLDCCIALRKLRQNESFALLAECFELSESDAEETFKRTIIKLARCLRPLICWPDANHFLNRLKHTPLDYRSDLMHLRSMIECVETDVSEALDFSCPSYKFILCINTNGIISYISSAYPGQCDDLQLFEASNFKNIVPDYLTLCADPGKAVPRSRQPVSDSIYEADEDDDSASLDEQRQPVSKYDAQRLSGQLASQESLSVVDGALVSKRAPSVQLPTMRLQEPACRAQMRATIDTLREYKMLHHSSIHQKSMLGYLNEMLIVAAALCNLKRKELSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWM4</accession>
    <name>A0A6I8UWM4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC4805697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001362055.3">
        <property type="nucleotide sequence ID" value="XM_001362018.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040820.2">
        <property type="nucleotide sequence ID" value="XM_015185334.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWM4"/>
    <dbReference type="KEGG" id="dpo:4805697"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="37"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001362055.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015040820.2"/>
        </source>
    </evidence>
    <sequence length="423" mass="47782" checksum="18CC104775F0B5F1" modified="2020-08-12" version="1">MSSSTVELAETHHKEQASVGNLLGRRYAELLHGEKYTDCVFHVCEEQLKCHKLILSAASPVFEAMFFGPMHENEPEIEIHDISAAIFKVLVDYIYTGSVDYNNLELVACIELYYAAEKYLLDQLIADSLVAITRKLRFSNILPALELSVCMGLDSLLEVCMTFFMRCCVSNAQYMTHLKEHYVHVSKECVKTIIAACKEPHKLLIWYVYEWTQQECEQLGLGPSDAGLVVHGLGPEANSWPVNASNEVQSPAPAPIVSVERCYYKACRPFTVDAESPVWRLRLKSPRFISLMGVVLNSRLPPNLTSTFGHVQLPLEYRESLRLDLCELPADGEDAPTNPVWSHVIQNQSTKYNCDLHLRWRREEACVLTPELSYELQIRWDGSAYGAEYPCSLQSCIADGIRFIDSDSFIGSLVKGLRYANLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWP7</accession>
    <name>A0A6I8UWP7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serine protease inhibitor 42Dd</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Spn47C</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001362018.3">
        <property type="nucleotide sequence ID" value="XM_001361981.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWP7"/>
    <dbReference type="FunCoup" id="A0A6I8UWP7">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805656"/>
    <dbReference type="InParanoid" id="A0A6I8UWP7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19954">
        <property type="entry name" value="serpin42Dd-like_insects"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="5">
        <location>
            <begin position="55"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001362018.3"/>
        </source>
    </evidence>
    <sequence length="417" mass="46362" checksum="FC400A08510D1268" modified="2020-08-12" version="1">MRRSVIGERRSKSVPLRAPDPNMPGEIVLLLCLIPMAAAGTTAPSLSAGPIVFARNLFRALNEDSPRGNMVVSPAAARSALTLVFMGAGGKSANELRSTLMLGAAGKKDIAKQHAEFWSRECTCSERGAALRLITRLYVSSDLPLRPDFNLKAVEFFNAQADALNYSDADASTRQVNRWLELQTFYTVRNLLTPASFSPESSVLLVNSIYFRAKWAKRFSMDRTGAGDFWINPAQRMELLMMRQVGEFRFADSKKLKASILHLPFEDSDISMMLVVPHAVDGLPELEQKLALLDLNEVATKGLLHEVDVVMPQFKIECDIDLKVPMQKMGISRIFSPGQADLSGLFAKKSPQLISEARQKLYLQVNESGCETDPDTPARAAAFVQNPDRKLFMANRPFVFAIRNNRTVYFVGRFLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWT9</accession>
    <name>A0A6I8UWT9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 209-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132230.2">
        <property type="nucleotide sequence ID" value="XM_002132194.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWT9"/>
    <dbReference type="KEGG" id="dpo:6903349"/>
    <dbReference type="InParanoid" id="A0A6I8UWT9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UWT9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019176">
        <property type="entry name" value="Cytochrome_B561-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21780">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21780:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09786">
        <property type="entry name" value="CytochromB561_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132230.2"/>
        </source>
    </evidence>
    <sequence length="493" mass="55287" checksum="4A673B234D99AEBD" modified="2020-08-12" version="1">MNRSRNKCSPMLQKSLDMRLRGAQAKAYLKRGAFNVLVLLLLLLHLSVACRCQRRGYFFAAECAVATVVALNAVACLAKYSWLWLSGVHGIGTYAQKCLLDGYDGNSFSTICVQKEKKPRCPEVEPCFKDYVINWHSSFEDSRCRPRRSLGRTPQRQSGCAEAPPDDCLTDVRRLPALMKSARRHRRSSEIADGPSAQPTSPGNAFWRCCNNQSIEPRTIYQLAPPPSPACAENAPTTFEEFSSFQFKDSSSEVIGRIATDKLPQYVANLRHWISTTVLQRLVSEIDNVDKGFRQRGLCDMKIGAVGLERLRVAAECAPTLPTLLLFLEGFSNQEYLVQRIRDLSQGSRLANFGRHDPEPASNLPSDAAIVFHLFCAYLDSQLMLTHQPRGGWRPFRHYYVLVRDDRRSLAAKDLALAVHNQAQCAILCSGSPLDPKFNFISGRELHSCAYDRNHLFCVILQFLLYMRQRQGSSLEGVSLGKGGINIMCVIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXE2</accession>
    <name>A0A6I8UXE2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein trunk</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trk</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the noggin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001382209.3">
        <property type="nucleotide sequence ID" value="XM_001382172.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXE2"/>
    <dbReference type="FunCoup" id="A0A6I8UXE2">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811830"/>
    <dbReference type="InParanoid" id="A0A6I8UXE2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXE2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052876">
        <property type="entry name" value="Insect_Hormone_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008717">
        <property type="entry name" value="Noggin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39940:SF4">
        <property type="entry name" value="PROTEIN TRUNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39940">
        <property type="entry name" value="PROTHORACICOTROPIC HORMONE, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05806">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026274239" evidence="6">
        <location>
            <begin position="24"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001382209.3"/>
        </source>
    </evidence>
    <sequence length="228" mass="26710" checksum="C8D37D8343B86DED" modified="2020-08-12" version="1">MKFYTKFNWLCIYFAISAFGVTSQEDYCAELSTQSLSRILGQAFNPRYMSIDPPGGPHEESYNLGYKRSSYEIPFYADSDVVSLGNFPAWETNHFAYFGKREEPIKGRKIRIRSPVVERGGNPNIDVFKHRPWECSSKINWIDLGLNYFPRYIRSIECISRKCWYGHFNCKPKSFTVKVLRRKSGSCIQINDKLVLITAEKFKNDYAQLWIWEEIAVNFCCECVMLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYM1</accession>
    <name>A0A6I8UYM1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">G2/M phase-specific E3 ubiquitin-protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pie</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133466.3">
        <property type="nucleotide sequence ID" value="XM_002133430.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UYM1"/>
    <dbReference type="FunCoup" id="A0A6I8UYM1">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901932"/>
    <dbReference type="InParanoid" id="A0A6I8UYM1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UYM1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051188">
        <property type="entry name" value="PHD-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059102">
        <property type="entry name" value="PHD_PHF7/G2E3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420:SF42">
        <property type="entry name" value="G2_M PHASE-SPECIFIC E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26054">
        <property type="entry name" value="PHD_G2E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002133466.3"/>
        </source>
    </evidence>
    <sequence length="495" mass="56181" checksum="BCCF22E003C921AE" modified="2020-08-12" version="1">MGKQQCDICGKESEDKESDTLLFGDWMIRQTLRVHYFCLLLSTDLPQRGGDSSGILGFLLRDIRKEAVAAKKHICVYCHEKGASIACHKCHTRFHLDCSLSNRCTSEFCGDFRSYCDACTPLDDYKRQLLANPPQNKRCDICLKAILPFSLHNVAYGDCCRKGFAHRMCMRRYALASGYYLRCIWCRKEKFRDIIRQQSIFVPDRDASWERQKNAYQDLHHKHMRCDHKNCLCPKGRDYNKHGWIIFPCKLCASIGAHLKCLTGTLRLSRGSEPVEFKCDVCLEVEQKLLSNKQLSMLNASDTGITKQNVEMSFYVPKSNPNLPVDLNTQTQPVFSDEENSENSDVSGITVIASQRASCIATSKKDIPVSVEATKPSPVNILKETIVNIPDSPQTQLQSQLQSDHEYSPGLVIRQSFTCAGEPFFYLVTHEFDERGSGDCTGSCTLRFATDDPRLKDRSPEALQNLLITEEDIWCRDTDRGIFSLVHSKILSPCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ58</accession>
    <name>A0A6I8UZ58_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Salivary glue protein Sgs-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6901274</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133975.2">
        <property type="nucleotide sequence ID" value="XM_002133939.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ58"/>
    <dbReference type="KEGG" id="dpo:6901274"/>
    <dbReference type="InParanoid" id="A0A6I8UZ58"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026108699" evidence="2">
        <location>
            <begin position="20"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133975.2"/>
        </source>
    </evidence>
    <sequence length="226" mass="24542" checksum="BB21ADEF571A542B" modified="2020-08-12" version="1">MRAATLIIFGLLMVGCCLARPQDSSPSSTTTTTTTSTTTTPKPVEDKASSTTTTSSTTTTTTTTTPKPDPKTASPSTTTTSTTPSTTTSTTTPSPSTTTSSTTPSTTSTTTTTTTTPKPKDAGDEEAEKLLQQCAELSRRKKRGGSSSEEDSGEGKSSKSGRKERKQLKKMCKELKRQQERKQEKTQKGSLSELAQALRTYAQRHGQEDRHEHRQDQRHHHGHRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZR3</accession>
    <name>A0A6I8UZR3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein phosphatase</fullName>
            <ecNumber evidence="10">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6901761</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="10"/>
    <dbReference type="RefSeq" id="XP_002134431.2">
        <property type="nucleotide sequence ID" value="XM_002134395.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZR3"/>
    <dbReference type="KEGG" id="dpo:6901761"/>
    <dbReference type="InParanoid" id="A0A6I8UZR3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZR3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="255"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002134431.2"/>
        </source>
    </evidence>
    <sequence length="498" mass="53882" checksum="492210CCE7F306D6" modified="2020-08-12" version="1">MDRLVRLNAGIQRMETQLLTFVQMMIGKAASGGGHLIKIVVGGSGLVIRIVISGSGKMLDVVATRSARVVCRARLSFAELVGIAVVRGGNLFDVCCCRGAELFEQACISGDELIARITSIGAMLSADGEDLLEQAFVCGTHVYENAYTNGGRMINQATTTVGTTGSRLAHVLSTGGFRLVSRVTNGGVYLAQRAATFGGRQCMNPLRPLFRGYGRLRELVEEYEQEIIDAGKPETKDIHGWKMYGPKELRLLSVASGIPKKHAAWPRLGQCGEDAWFATSTSRGETLGVAKANGVKSGRICNLSPGDFSYSLMRSCERLAQRPSHDPRRLDVLLHRAHRDVLDVRHPVLASCNTCMLSLDRRTGTVYATNVGGCGFLVVRNGQIAARSRKHLQAFSTQLQGVGAYIYGDPYQAPIQELCVEAGDMLLLGTDGFFNNVDDERVLSLITELDGGTDPRRMHLYAETLALMARATACSNVRAAAGRNLNMDDITIVLAVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZS6</accession>
    <name>A0A6I8UZS6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutathione transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GstT3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Theta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="RefSeq" id="XP_002134314.2">
        <property type="nucleotide sequence ID" value="XM_002134278.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZS6"/>
    <dbReference type="FunCoup" id="A0A6I8UZS6">
        <property type="interactions" value="773"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901645"/>
    <dbReference type="InParanoid" id="A0A6I8UZS6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03183">
        <property type="entry name" value="GST_C_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03050">
        <property type="entry name" value="GST_N_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000008">
        <property type="entry name" value="Glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000227">
        <property type="entry name" value="glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040077">
        <property type="entry name" value="GST_C_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040075">
        <property type="entry name" value="GST_N_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051369">
        <property type="entry name" value="GST_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917:SF8">
        <property type="entry name" value="GH16740P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="9">
        <location>
            <begin position="44"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="10">
        <location>
            <begin position="132"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="9"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002134314.2"/>
        </source>
    </evidence>
    <sequence length="269" mass="31279" checksum="0A7B1C79D8574046" modified="2020-08-12" version="1">MSSRLATLLGLSQGSEDQQHLQKAFEEGRKTPTRQTTNLKMSPSPIKYYYDLMSQPSRALFIIFRLSGMPFEDCVVALRNGEHLTDDFKRDINRFQRVPCINDNGNKLAESVAILRYLSAKGKIPEHLYPKYFVDQSRVDEFLEWHHISLRLTCAMYFRTVWLDPLLTGRTPTEAKIEMMRMHMERNLDVVEEVWLESSDFLTGSTLCVADIFAACEIEQTRMADYDVRIKYPKIRAWLKRVRQSCNPHYDVAHKFVYQISGTGPQAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V078</accession>
    <name>A0A6I8V078_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pupal cuticle protein Edg-78E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Cpr65Ec</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134825.3">
        <property type="nucleotide sequence ID" value="XM_002134789.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V078"/>
    <dbReference type="FunCoup" id="A0A6I8V078">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900434"/>
    <dbReference type="InParanoid" id="A0A6I8V078"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050468">
        <property type="entry name" value="Cuticle_Struct_Prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380">
        <property type="entry name" value="CUTICLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10380:SF237">
        <property type="entry name" value="CUTICULAR PROTEIN 65AU, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026011303" evidence="3">
        <location>
            <begin position="20"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002134825.3"/>
        </source>
    </evidence>
    <sequence length="129" mass="14044" checksum="00E7F5F7B6E3D843" modified="2020-08-12" version="1">MNKFLILVVATLALACAQARPDAYDAAAETREYKSDLKEDGSYNYQYQTSNGIAGQESGVGGYYASGSSAYYAPDGQLVQLTYTADENGYHPVGAHLPTPPPIPAAILKSLEYIRTHPHQEQRQGQGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3D7</accession>
    <name>A0A6I8V3D7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein 208</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6897568</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137713.4">
        <property type="nucleotide sequence ID" value="XM_002137677.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3D7"/>
    <dbReference type="FunCoup" id="A0A6I8V3D7">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897568"/>
    <dbReference type="InParanoid" id="A0A6I8V3D7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001370">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002403">
        <property type="entry name" value="zinc finger protein 420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF393">
        <property type="entry name" value="CHROMATIN-LINKED ADAPTOR FOR MSL PROTEINS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00868">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="15">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="244"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="332"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="577"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="606"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="744"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="804"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="849"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="882"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="910"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="938"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="196"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="268"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="357"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002137713.4"/>
        </source>
    </evidence>
    <sequence length="964" mass="112420" checksum="A5B149BD9BCB46C2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4J8</accession>
    <name>A0A6I8V4J8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6897872</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137974.1">
        <property type="nucleotide sequence ID" value="XM_002137938.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4J8"/>
    <dbReference type="GeneID" id="6897872"/>
    <dbReference type="KEGG" id="dpo:6897872"/>
    <dbReference type="InParanoid" id="A0A6I8V4J8"/>
    <dbReference type="OMA" id="HKADAQY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248873">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032004">
        <property type="entry name" value="DUF4790"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16037">
        <property type="entry name" value="DUF4790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002137974.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="21770" checksum="0CD876EA0FAE2A0E" modified="2020-08-12" version="1">MSTRESISSRKSLVVGVRSTQEQAAIARYSRWTASVTIDENFTSGFPDMSRRHEKDKQHIVPGYQDLPEVKYDPKFTPPYCDDLEVFIDPNFATFRVPLYVRCLERYGEAKRRYNRQLNREVKKLIENQPTADCAWQFVRTMSYTTLWPPLHTREELKLFEKGFCKLSPAEKRHYDKIMNTNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4R0</accession>
    <name>A0A6I8V4R0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Enoyl-[acyl-carrier-protein] reductase, mitochondrial</fullName>
            <ecNumber evidence="11">1.3.1.104</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">2-enoyl thioester reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6898804</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.104"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.104" evidence="11"/>
    <dbReference type="RefSeq" id="XP_002138778.2">
        <property type="nucleotide sequence ID" value="XM_002138742.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4R0"/>
    <dbReference type="FunCoup" id="A0A6I8V4R0">
        <property type="interactions" value="2241"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898804"/>
    <dbReference type="InParanoid" id="A0A6I8V4R0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08290">
        <property type="entry name" value="ETR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000112">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000010">
        <property type="entry name" value="Enoyl-[acyl-carrier-protein] reductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051034">
        <property type="entry name" value="Mito_Enoyl-ACP_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43981:SF2">
        <property type="entry name" value="ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="15">
        <location>
            <begin position="35"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002138778.2"/>
        </source>
    </evidence>
    <sequence length="356" mass="39334" checksum="84679A5D1D1D74FD" modified="2020-08-12" version="1">MLRQTLRPCHAAHWCRQMSVVAKSLKYTQHGEPQDVLKLVEDQLADPKDKQVLVRILAAPINPADINTIQGKYPVKPKFPAVAGNEFVGEVICVGENVKGLQAGEHVIPLATGLGTWTTYGLYNQNQLMTVPKKVGLAEAATVTVNPCTAYRMLKDFVKLSPGDTVIQNGANSAVGQAVHQLCRAWGINSIGIVRDRPEICELKQLLECLGATAVLTEAEIRTSDIFKTGKVKRPKLAFNCVGGKSATEVSRHLDDRGVMVTYGGMSREPVTVATGPLIFKDIAFRGFWMTRWSKENYDAPERKQMFKELFELMEQGKFVAPTHEMVPLEKFKDAAAAALSFKGFTGKKFILNMRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4S1</accession>
    <name>A0A6I8V4S1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase 1 regulatory subunit 36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6897978</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138077.2">
        <property type="nucleotide sequence ID" value="XM_002138041.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:6897978"/>
    <dbReference type="InParanoid" id="A0A6I8V4S1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Pro_pase_1_reg_su_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21055">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21055:SF3">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14895">
        <property type="entry name" value="PPPI_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002138077.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="54804" checksum="9FB50F785A04191E" modified="2020-08-12" version="1">MLRLHVDDYSPKYYSGKWSWDWKKDALHFEPHNDLSDAKERYITTNSFKFLRTMNQEEELVFRHVFARADTTYDTNSVVLNDIRDLVLFFMPSEFLSYKFVEFMHHRAVYHLLHSLTIYFEYYLRMVEFVMIRRDELSGQMGQIQSEQTNDMKRVFSVYLSQYRMLVARNYSIIVKAEGDLGKFYHMKVVNISATIWEKNFHEQLLACSTQIVWIAMHRRAYNIIEMEMNRLFRSDYFVTARTEYPTFSPVERSLLYGRSSKNMNYRKQVSPLIQELENPKEEDLPILWIEERKYRGTNIRIMEIELEYIVPPPQLRLIDVAHGILGHPRKLYNTILELDWSAVRYCNLSMKHDPYFMVRQPRLKIPKIDEMKRHQMAETYETFYKINEIYDLPSHAIYLKWVKRDKLLENYRAGGLLTSNYLRAENELNRTSLGPSVDQIISNYRKAMVKHRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4V0</accession>
    <name>A0A6I8V4V0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138842.1">
        <property type="nucleotide sequence ID" value="XM_002138806.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4V0"/>
    <dbReference type="GeneID" id="6898871"/>
    <dbReference type="KEGG" id="dpo:6898871"/>
    <dbReference type="InParanoid" id="A0A6I8V4V0"/>
    <dbReference type="OMA" id="EIKHDQP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245569">
        <property type="expression patterns" value="Expressed in adult organism and 1 other cell type or tissue"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026315285" evidence="1">
        <location>
            <begin position="16"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138842.1"/>
        </source>
    </evidence>
    <sequence length="192" mass="21115" checksum="3503B1FC34FC9C98" modified="2020-08-12" version="1">MKAVIILVLVGTVLGVPQIKVRPHSEPHAHIFAEVRPAGQKHQQQGVDMEIKEQHLVVETKPAHQESAASHVVEAVFKPAAESHVVEVEIKQAEQKPQHVELDIKPVHESASHVVELEVKQAADHQKPQHIEVELVAGHKGVQSHHEVEVEISPEQQPPHQIEVEIVKGVASEVKPPLPLHSGQQLSLNLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V766</accession>
    <name>A0A6I8V766_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4802454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736739.3">
        <property type="nucleotide sequence ID" value="XM_003736691.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V766"/>
    <dbReference type="FunCoup" id="A0A6I8V766">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802454"/>
    <dbReference type="InParanoid" id="A0A6I8V766"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V766">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd01214">
        <property type="entry name" value="PTB_FAM43A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033930">
        <property type="entry name" value="FAM43A/B_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF2">
        <property type="entry name" value="FI05246P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14719">
        <property type="entry name" value="PID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="2">
        <location>
            <begin position="280"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="669"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="770"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="679"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="868"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_003736739.3"/>
        </source>
    </evidence>
    <sequence length="885" mass="98171" checksum="C873438ECFA8D0AD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VEX7</accession>
    <name>A0A6I8VEX7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26531972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039330.1">
        <property type="nucleotide sequence ID" value="XM_015183844.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VEX7"/>
    <dbReference type="GeneID" id="26531972"/>
    <dbReference type="KEGG" id="dpo:26531972"/>
    <dbReference type="InParanoid" id="A0A6I8VEX7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0270959">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015039330.1"/>
        </source>
    </evidence>
    <sequence length="121" mass="13592" checksum="3BBBC54DFE20F251" modified="2020-08-12" version="1">MSINLYIQTLNTTLQTIATQVEVFAGVALKAAVNVRRELESLTEDWHWQNSLIEFTDLQATLLKVLLVLLAGTCILNAGSWSVYGEVITEKFVRPSTLKEIEELKLSVAKLKLPKEHSPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFX5</accession>
    <name>A0A6I8VFX5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039957.2">
        <property type="nucleotide sequence ID" value="XM_015184471.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFX5"/>
    <dbReference type="KEGG" id="dpo:26533256"/>
    <dbReference type="InParanoid" id="A0A6I8VFX5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015039957.2"/>
        </source>
    </evidence>
    <sequence length="142" mass="15983" checksum="4F364D87111A63B3" modified="2020-08-12" version="1">MIFLGSFFCLLSLYFGCLIIGVTGLIIGVASLTLAVCKLILHAKQEEVWMMALIFSLLYLGAKMFLLMGTMWNLAWCLIMSFIASAVCVCLILAILIVGFASSANRVQLMVWITMILLETYYLLVIISHWYNIWSGVQRVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VH00</accession>
    <name>A0A6I8VH00_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Diuretic hormone receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Dh44-R2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for the insect diurectic hormone. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015040662.2">
        <property type="nucleotide sequence ID" value="XM_015185176.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VH00"/>
    <dbReference type="FunCoup" id="A0A6I8VH00">
        <property type="interactions" value="303"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VH00"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008036">
        <property type="term" value="F:diuretic hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15263">
        <property type="entry name" value="7tmB1_DH_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000155">
        <property type="entry name" value="diuretic hormone receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000023">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002001">
        <property type="entry name" value="GPCR_2_diuretic_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF15">
        <property type="entry name" value="DIURETIC HORMONE 44 RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01127">
        <property type="entry name" value="DIUHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="14">
        <location>
            <begin position="57"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="164"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015040662.2"/>
        </source>
    </evidence>
    <sequence length="496" mass="56453" checksum="9FA706F12A72C408" modified="2020-08-12" version="1">MADDDLRALVDSLDDASPENLANVIANFSLDMLQRASALIGAQQPHSGALLINRTQQCQQQSQQEDELEATATAAAAAAGGKRILHCPSAFDSVLCWPRTKAATLAVLPCFEEFKGVHYDTTENATRYCFENGTWDHYSDYDRCHQQSGSVPMVPDFSPSVDLPAIIYACGYFISFATLVVALIVFLSFKDLRCLRNTIHANLFLTYITSALLWILTLFLQVITTESSQAGCITLVIMLQYFYLTNFFWMFVEGLYLYTLVVQTFSSDNISFIIYALIGWGCPAIFIMAWAIAKAFAPHLENDHFNGLEIDCGWMRESHIDWIFKGPASVALLVNLVFLIRIMWVLITKLRSAHTLETRQYYKASKALLVLIPLFGITYLLVLTGPEQGISRNLFEAIRAFLISTQGFFVALFYCFLNSEVRQTLRHRFIRWRESRNIHRSSSIKNRRHRASKDYSQRSRTESLRTEECVICLRPTPHTRLATQEQYHSINITDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VK55</accession>
    <name>A0A6I8VK55_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Bromodomain-containing protein DDB_G0280777 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nuf</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015043020.2">
        <property type="nucleotide sequence ID" value="XM_015187534.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VK55"/>
    <dbReference type="FunCoup" id="A0A6I8VK55">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900625"/>
    <dbReference type="InParanoid" id="A0A6I8VK55"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VK55">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2440:FF:000003">
        <property type="entry name" value="Nuclear fallout, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037245">
        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057316">
        <property type="entry name" value="Rab11-FIP3/4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051977">
        <property type="entry name" value="Rab11-interacting_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726:SF7">
        <property type="entry name" value="NUCLEAR FALLOUT, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726">
        <property type="entry name" value="RAB11-FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25450">
        <property type="entry name" value="Rab11-FIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09457">
        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144270">
        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51511">
        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FIP-RBD" evidence="10">
        <location>
            <begin position="489"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="248"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015043020.2"/>
        </source>
    </evidence>
    <sequence length="556" mass="62365" checksum="CFF2EABB402DBC50" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLH9</accession>
    <name>A0A6I8VLH9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">Gk2</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15">MV-25-SWS-2005</strain>
            <tissue evidence="14 15">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001352753.2">
        <property type="nucleotide sequence ID" value="XM_001352717.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043814.2">
        <property type="nucleotide sequence ID" value="XM_015188328.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLH9"/>
    <dbReference type="KEGG" id="dpo:4812321"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VLH9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07792">
        <property type="entry name" value="ASKHA_NBD_FGGY_GK1-3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000086">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000108">
        <property type="entry name" value="Glycerol kinase, glycosomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042018">
        <property type="entry name" value="GK1-3_metazoan-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000756">
        <property type="entry name" value="PRK00047.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF40">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 10 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="11">
        <location>
            <begin position="33"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="12">
        <location>
            <begin position="299"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001352753.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015043814.2"/>
        </source>
    </evidence>
    <sequence length="576" mass="64464" checksum="D1DB11D6A55A5726" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VM60</accession>
    <name>A0A6I8VM60_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">isocitrate dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="2">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Isocitric dehydrogenase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NAD(+)-specific ICDH subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4812701</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="2"/>
    <dbReference type="RefSeq" id="XP_015044137.2">
        <property type="nucleotide sequence ID" value="XM_015188651.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VM60"/>
    <dbReference type="KEGG" id="dpo:4812701"/>
    <dbReference type="InParanoid" id="A0A6I8VM60"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VM60">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000014">
        <property type="entry name" value="Isocitrate dehydrogenase (NAD(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="8">
        <location>
            <begin position="501"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="122"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="279"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="415"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015044137.2"/>
        </source>
    </evidence>
    <sequence length="836" mass="86585" checksum="DE86AED1ABFCA224" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQF4</accession>
    <name>A0A6I8VQF4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Potassium voltage-gated channel unc-103 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">sei</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033233281.1">
        <property type="nucleotide sequence ID" value="XM_033377390.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233282.1">
        <property type="nucleotide sequence ID" value="XM_033377391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQF4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000020">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000107">
        <property type="entry name" value="Potassium voltage-gated channel unc-103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003967">
        <property type="entry name" value="K_chnl_volt-dep_ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF548">
        <property type="entry name" value="GH12235P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01470">
        <property type="entry name" value="ERGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="560"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="14">
        <location>
            <begin position="663"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033233281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033233282.1"/>
        </source>
    </evidence>
    <sequence length="862" mass="98947" checksum="3950B2ADAEC0BD76" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQH8</accession>
    <name>A0A6I8VQH8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-soluble NSF attachment protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-ethylmaleimide-sensitive factor attachment protein gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gammaSnap1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233297.1">
        <property type="nucleotide sequence ID" value="XM_033377406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQH8"/>
    <dbReference type="FunCoup" id="A0A6I8VQH8">
        <property type="interactions" value="1790"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117183508"/>
    <dbReference type="InParanoid" id="A0A6I8VQH8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000477">
        <property type="entry name" value="gamma-soluble NSF attachment protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF2">
        <property type="entry name" value="GAMMA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033233297.1"/>
        </source>
    </evidence>
    <sequence length="300" mass="33821" checksum="3251A40CF6B03C8B" modified="2020-08-12" version="1">MSAAKKVAEAEELVRQAEKSLKTSLLKWVPDYDSAADEYSKAATAFRIAKNFDKSKVYQLKAIECYKNNKSWFHAAKCYEQIVLLSKDTDKLLEIEEYVNKACSLYQQHGSPEAAASALDKAAKLTEQKHPEMALRFYQHAIEVIMIDDSIRQATEYSSKVSRILVKLRRYDEAAKAILKEMDLNQQTESYGQIGRLVVALVLVQLARGDSVEAEKAFKEWGSCCEPEEVSTLQTLLNAFDEEDAEMASRMLATPFIRHMDVEYAILAKNIPLPEGMQLEKKSEDNAGIATNEDEDEGIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQT0</accession>
    <name>A0A6I8VQT0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Cytochrome c oxidase subunit 1</fullName>
            <ecNumber evidence="14">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC117183516</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:11437"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Mitochondrion inner membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="14"/>
    <dbReference type="RefSeq" id="XP_033233392.1">
        <property type="nucleotide sequence ID" value="XM_033377501.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VQT0">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117183516"/>
    <dbReference type="InParanoid" id="A0A6I8VQT0"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="11 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026342028" description="Cytochrome c oxidase subunit 1" evidence="16">
        <location>
            <begin position="22"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="17">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033233392.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="48281" checksum="F384BAD44EE846E2" modified="2020-08-12" version="1">MIFFIVIPIIIGNXXVPLILGAPEIAFPRIKNIREXLLPPALSLLLVRRIVENGAGTGXTVYPPLSSGIAHGGASVDLAICSLHLAGISSILGAVHFITTVIHIRYSGITLDRIPLFMXSVVITALLLLSLPVLAGAITILLTDRNLNTYFFDPAGGGDPILYQHLFXFFGHPEVYILILPGFGIISHIISQESGKKETFASLGIIYAILAIGLLGFIVXAHHIFTVGIDVDTRAYFTSATIIIAVPTGIKIFRXLATLHGAQLSYSPAILXALGFVFLFTVGGLTGVVLANSSVDIILHDTYYVVAHFHYVLSMGAVFAIIAGFIHXFPLFTGLTINNKLLKSQFVIIFIGVNLTFFPHHFLGLAGMPRRYSDYPDAYTTXNVVSTIGSSISLLGILFLFYIIXESLVSQRQVIYPIQLCSSIEXYQNTPPAEHSYSELPLLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VR34</accession>
    <name>A0A6I8VR34_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine/threonine-protein kinase DDB_G0283821</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sqa</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233521.1">
        <property type="nucleotide sequence ID" value="XM_033377630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VR34"/>
    <dbReference type="FunCoup" id="A0A6I8VR34">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898797"/>
    <dbReference type="InParanoid" id="A0A6I8VR34"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VR34">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14103">
        <property type="entry name" value="STKc_MLCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000534">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000594">
        <property type="entry name" value="Myosin light chain kinase isoform-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF20">
        <property type="entry name" value="MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
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    <sequence length="867" mass="95812" checksum="B40BD234DC75928A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVC7</accession>
    <name>A0A6I8VVC7_DROPS</name>
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        <lineage>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="term" value="P:negative regulation of translation"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCR4-NOT transcription complex subunit 1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="1.25.40.840:FF:000001">
        <property type="entry name" value="Ccr4-not transcription complex subunit 1 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.790:FF:000004">
        <property type="entry name" value="Not1, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055104">
        <property type="entry name" value="CNOT1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22940">
        <property type="entry name" value="CNOT1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23590">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 N-terminal" evidence="13">
        <location>
            <begin position="31"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="12">
        <location>
            <begin position="598"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="11">
        <location>
            <begin position="936"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="10">
        <location>
            <begin position="1193"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="9">
        <location>
            <begin position="1562"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="14">
        <location>
            <begin position="1785"/>
            <end position="1987"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="8">
        <location>
            <begin position="2182"/>
            <end position="2540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="370"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="782"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="903"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1439"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="904"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="925"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1448"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1488"/>
            <end position="1512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033234569.1"/>
        </source>
    </evidence>
    <sequence length="2569" mass="287933" checksum="AB3FF01EA5B363A5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVZ7</accession>
    <name>A0A6I8VVZ7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4729 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117183909</name>
        <name evidence="3" type="synonym">LOC4816456</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356125.4">
        <property type="nucleotide sequence ID" value="XM_001356089.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235251.1">
        <property type="nucleotide sequence ID" value="XM_033379360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VVZ7"/>
    <dbReference type="KEGG" id="dpo:117183909"/>
    <dbReference type="KEGG" id="dpo:4816456"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031732">
        <property type="entry name" value="DUF4729"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15866">
        <property type="entry name" value="DUF4729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4729" evidence="1">
        <location>
            <begin position="52"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356125.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235251.1"/>
        </source>
    </evidence>
    <sequence length="264" mass="29037" checksum="146940D0E5056F8F" modified="2020-08-12" version="1">MSARSIQMNHIIIYSQAEFCVQYSTMPTHGDLCTSEMLAFLDVEQIFNRPLKCPVPFCGSELTPIEMLTHLLMGHSPGESMLETVANCPELFHVQLNELKAGTSYVIAAIAYHGTPKVGLSTPVSPELQIVHQLPVMLVMYVTPLANKHNGIDTDNAEQTVIFYMVSPLMATEVGVQITLLDGDQDMPGQKALCQFSAHTQTGAYGLGFGEVEQLLVHMEFVFYSASSIQKLIDADPERQLHVKVVLTGEPNSLGSESHPEYPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VW74</accession>
    <name>A0A6I8VW74_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD repeat domain phosphoinositide-interacting protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Atg18b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235335.1">
        <property type="nucleotide sequence ID" value="XM_033379444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VW74"/>
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        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VW74"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000529">
        <property type="entry name" value="WD repeat domain phosphoinositide-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235335.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="54906" checksum="CF13AEA10BD66BF5" modified="2020-08-12" version="1">MRNCLLEVFVALEFPTSSGCAGMEKAMLSSVRLSLRSGNTVTDEVLLSSLSVLSPAGLRLYSIAGQDKVEEIFAKDNTEQIRIVERLFNSSLVVLVTSQKPNCLKMLHFKKKQDICNCFYPSEILCVRMNRQRLIVCLAESIHIHDIRDMKILHSIENIAPNELGLCALSLNSHLAFPVCQSSGELRIFNANKLRTGMTIKAHDTPLSALTFSPSGALLATASERGTVIRVFCVKNGQRVQEFRRGVSCVRIASLVFAASGDFLCASSNTETVHIFKIDARAVESVELKAIAEVAAKSDKASKESAASAPPDTEEATATPVASWGGMFSKAVSSLLHSTQVSDVLAQDRSFATVVLAQAGLKHICALTRVQKELRLLIACEDGFLYVHEFNAERGGSCKLLAVHDLRGALEDVIELQLSESVLKMQAKPVTTPQSLTLLKSCAASVLIENPDPMPDNSYASILRGDQADAMSDSAKFRKLCDAIDTPTKLYDERQFPPVAIAAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWY3</accession>
    <name>A0A6I8VWY3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(+) kinase</fullName>
            <ecNumber evidence="2">2.7.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Nadk1a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033235084.1">
        <property type="nucleotide sequence ID" value="XM_033379193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWY3"/>
    <dbReference type="FunCoup" id="A0A6I8VWY3">
        <property type="interactions" value="2167"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VWY3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077342">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VWY3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000003">
        <property type="entry name" value="NAD kinase b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000013">
        <property type="entry name" value="NAD kinase isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033235084.1"/>
        </source>
    </evidence>
    <sequence length="939" mass="104502" checksum="D2122255D8620861" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VX88</accession>
    <name>A0A6I8VX88_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein hook</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC117183994</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytic trafficking by stabilizing organelles of the endocytic pathway. Probably acts as a cytoskeletal linker protein required to tether endosome vesicles to the cytoskeleton. Involved in modulation of endocytosis at stages required for down-regulation of membrane proteins that control synapse size. Not involved in synaptic vesicle recycling. Required in R7 cells for boss endocytosis into multivesicular bodies (MVBs). Has a role in regulating adult longevity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Interacts with microtubules via its N-terminus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the hook family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235700.1">
        <property type="nucleotide sequence ID" value="XM_033379809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VX88"/>
    <dbReference type="KEGG" id="dpo:117183994"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070526">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VX88">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22222">
        <property type="entry name" value="HkD_Hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000024">
        <property type="entry name" value="Hook homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008636">
        <property type="entry name" value="Hook_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043936">
        <property type="entry name" value="HOOK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF39">
        <property type="entry name" value="PROTEIN HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05622">
        <property type="entry name" value="HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19047">
        <property type="entry name" value="HOOK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="201"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033235700.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="34894" checksum="CDD8ADBE039F3B57" modified="2020-08-12" version="1">MSAAKNEMYYSLLEWFKTLNLNAPHADAESLADGVAVAQALNQFAPESFTDSWLAKIKASAVGINWRLRMSNLKKVTQSLYDYYSEVLNYTLSDFVKPDVQRIAEKCDLVELERLLQLVLGCAVNCAKKQSYITEIMCLEEELQANIMRALQELESSRNAAEGGIVTSLSRSSISGMLDGKVLQEERDAMAQKCFETEKKMLLLIDEKTNLQQELQRVQKEFARLEHSSTVIGDDGVSLGPVQTGSVRYNELRRQLDLLKEELLQSEGAREDLKLKAQQQETDLLHMQMRIDELLVGISKFKYILSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXD5</accession>
    <name>A0A6I8VXD5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7 8">Chaoptin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8 9" type="primary">LOC4818057</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7 8">MV-25-SWS-2005</strain>
            <tissue evidence="6 7 8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235716.1">
        <property type="nucleotide sequence ID" value="XM_033379825.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235717.1">
        <property type="nucleotide sequence ID" value="XM_033379826.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235718.1">
        <property type="nucleotide sequence ID" value="XM_033379827.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235719.1">
        <property type="nucleotide sequence ID" value="XM_033379828.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4818057"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634576" evidence="4">
        <location>
            <begin position="18"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="226"/>
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            <begin position="239"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
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            <dbReference type="RefSeq" id="XP_033235717.1"/>
        </source>
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            <dbReference type="RefSeq" id="XP_033235718.1"/>
        </source>
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        <source>
            <dbReference type="RefSeq" id="XP_033235719.1"/>
        </source>
    </evidence>
    <sequence length="1346" mass="152115" checksum="DDF826822047B0EA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W096</accession>
    <name>A0A6I8W096_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC26534265</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_033380792.1"/>
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        <property type="component" value="Chromosome 4"/>
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            <begin position="1"/>
            <end position="33"/>
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    </feature>
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            <end position="319"/>
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    </feature>
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        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
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        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
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            <end position="304"/>
        </location>
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        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236683.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="43228" checksum="5B87E72769F41719" modified="2020-08-12" version="1">MYKNTKEDRVRKRLKTPSRVYQSKVQLGHQQEKVSLEEPNLKILPDKLSIIKNSATHLEPKQSHLQNAAEATLKQLEGPTVKPELKIQPDELPVIKRSETHLKPRQGHLQKALKAKLKMEHQQEKGPPVKPELKILPDKLCEIKRSETHLETKDSHPQNVNEIKCILEEEKEGKDVSFTEFIEKQGWGSFSMDFLKDLNGNKSQRVHHSSRLSPRTNIDKLHTARMDFKSIDCFESVESNNRIIFPSSYCAHSDVDNDYVAKQRAKWTDFFAQQKKTPMELRLEERRRQIQKKESVKRANEQQRTKQTRSKIQPRRSLSALYREPRQTHRMRKAMESIAEELAAMNIQPEESGECSHGYICSGESSCSQSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W128</accession>
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        <submittedName>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <strain evidence="11 12">MV-25-SWS-2005</strain>
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        <subcellularLocation>
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        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="CTF/NFI"/>
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        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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            <end position="212"/>
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        </source>
    </evidence>
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            <dbReference type="RefSeq" id="XP_033237057.1"/>
        </source>
    </evidence>
    <sequence length="791" mass="87589" checksum="619316E8A6AEC33E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W270</accession>
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        <submittedName>
            <fullName evidence="11">Uncharacterized protein Ir7g</fullName>
        </submittedName>
    </protein>
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    </gene>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="11">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF30">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="387"/>
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            <begin position="579"/>
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            <begin position="19"/>
            <end position="210"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Pfam" id="PF24061"/>
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    <evidence type="ECO:0000313" key="11">
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        </source>
    </evidence>
    <sequence length="610" mass="70466" checksum="87ED5017D779D532" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3V3</accession>
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        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
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        <name evidence="12" type="primary">nod</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033237993.1">
        <property type="nucleotide sequence ID" value="XM_033382102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W3V3"/>
    <dbReference type="FunCoup" id="A0A6I8W3V3">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W3V3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12836">
        <property type="entry name" value="HHH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="8"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="518"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="86"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033237993.1"/>
        </source>
    </evidence>
    <sequence length="748" mass="83384" checksum="925EDCE9D8B04CCD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5H1</accession>
    <name>A0A6I8W5H1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Facilitated trehalose transporter Tret1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">LOC4801931</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Trehalose transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358949.2">
        <property type="nucleotide sequence ID" value="XM_001358912.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238620.1">
        <property type="nucleotide sequence ID" value="XM_033382729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5H1"/>
    <dbReference type="KEGG" id="dpo:4801931"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000055">
        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF47">
        <property type="entry name" value="GH17672P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="45"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358949.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033238620.1"/>
        </source>
    </evidence>
    <sequence length="491" mass="53329" checksum="46B497483338A290" modified="2020-08-12" version="1">MPSVAREEETGVKYSYTEVSSFQISEVNTRNMSPEPVKTGRIFMAAVAANLSAFVVGTCLGWTSPIGPKLKTEDTSDSPLDRPITSDEDAWISSLIAIGALIAPFVAGPLADRIGRKWVLLSSSLFFVLAFGINMGASEVWILYLSRLIQGFGVGFVMTVQPMYVGEISTDNVRGATGSLMQLFIVAGILYVYAIGPFVSYMALQWCCIVVPVIFDAIFYFMPESPHYYAGKGRKTDALRSLQFLRGQSAEGVHNEMAEIQSSVEEAMANKGTIMDLFKNPGNRKALFICAGLISFQQLSGINVVLFNSQSIFASANTGLDPAIATIIIGCVQVASSGLTPIVADRLGRKVMLLTSASVMTVGLTALGAFFYMQLVVGDISEVVWMPVPALVIYNIVYCTGFGPLPWAVLGEMFPANIKSAASSIVASTCWTLGFLVTYFYPALDALGSYYAFWLFAGFMVVAFFFVLFVVMETKGLSLQEIQDRLNSKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5K9</accession>
    <name>A0A6I8W5K9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7 8">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8 9" type="primary">LOC6901037</name>
    </gene>
    <organism evidence="5 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7 8">MV-25-SWS-2005</strain>
            <tissue evidence="6 7 8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042219.2">
        <property type="nucleotide sequence ID" value="XM_015186733.2"/>
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    <dbReference type="RefSeq" id="XP_015042220.2">
        <property type="nucleotide sequence ID" value="XM_015186734.2"/>
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        <property type="nucleotide sequence ID" value="XM_015186737.2"/>
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        <property type="nucleotide sequence ID" value="XM_033382745.1"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008277">
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        <property type="project" value="InterPro"/>
    </dbReference>
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    <dbReference type="CDD" id="cd08705">
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        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
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    </dbReference>
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        <property type="entry name" value="G-protein_gamma-like_dom"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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        <property type="entry name" value="LP21163P"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746:SF5">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="RGSPROTEIN"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00224">
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0734">Signal transduction inhibitor</keyword>
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        <location>
            <begin position="256"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="530"/>
            <end position="646"/>
        </location>
    </feature>
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        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="675"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1013"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1159"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1249"/>
            <end position="1327"/>
        </location>
    </feature>
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        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="70"/>
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    </feature>
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        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
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        <location>
            <begin position="160"/>
            <end position="197"/>
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            <end position="1307"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015042219.2"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015042220.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015042223.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033238636.1"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A6I8W7M8</accession>
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        <submittedName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF299">
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    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
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            <end position="72"/>
        </location>
    </feature>
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        <location>
            <begin position="536"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="13">
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            <begin position="549"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033239368.1"/>
        </source>
    </evidence>
    <sequence length="561" mass="64991" checksum="DD6302A5F253B376" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7W5</accession>
    <name>A0A6I8W7W5_DROPS</name>
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        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="primary">LOC117184744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239458.1">
        <property type="nucleotide sequence ID" value="XM_033383567.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8W7W5"/>
    <dbReference type="KEGG" id="dpo:117184744"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042575">
        <property type="term" value="C:DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF4">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="649"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239458.1"/>
        </source>
    </evidence>
    <sequence length="960" mass="109711" checksum="33B0AE83084E80D4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8C4</accession>
    <name>A0A6I8W8C4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vitellogenin receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6900911</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239623.1">
        <property type="nucleotide sequence ID" value="XM_033383732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W8C4"/>
    <dbReference type="KEGG" id="dpo:6900911"/>
    <dbReference type="InParanoid" id="A0A6I8W8C4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239623.1"/>
        </source>
    </evidence>
    <sequence length="89" mass="9987" checksum="E2A09760378E2E2E" modified="2020-08-12" version="1">MKWDVPYDCQFSCQSGGCFDKALICDGTTCDNCENVHDEADCCKLPGDFQCAMVQTICLPRIFLRTEQDEEGAERLGQIKQKQGLKTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8I0</accession>
    <name>A0A6I8W8I0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="4 17">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SdhBL</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="17">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="17">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion inner membrane</location>
            <topology evidence="1 17">Peripheral membrane protein</topology>
            <orientation evidence="1 17">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="4 17"/>
    <dbReference type="RefSeq" id="XP_033239683.1">
        <property type="nucleotide sequence ID" value="XM_033383792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W8I0"/>
    <dbReference type="FunCoup" id="A0A6I8W8I0">
        <property type="interactions" value="654"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815149"/>
    <dbReference type="InParanoid" id="A0A6I8W8I0"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W8I0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit SdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="15 17" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="7 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 17" id="KW-0408">Iron</keyword>
    <keyword evidence="14 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="19">
        <location>
            <begin position="129"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="261"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="49"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_033239683.1"/>
        </source>
    </evidence>
    <sequence length="375" mass="42057" checksum="2255B0CC873BE72A" modified="2020-08-12" version="1">MFLVRRTTNVLKFRSKIQLVRSIICPEPSIRVLDDEQEYVRAGQAILTKGQTPAPPPLPPQPPPPAMVPSTEGPPTVATAAAGGDKKPPPKGPAPPKEPLKPPPAVAEKKKEAGPPAPPKPKEKRIKNFEIYRWQPGEQPKMQTYAIDLSQCGAMVLDALIKIKNEVDQTLTFRRSCREGICGSCAMNIDGTNTLACVTPINQSTSGSCRIYPLPHLYVVRDLVPDLTQFYDQYRSIQPWLQRNNIQNEMGKAQYLQSLEDRNKLDGLYECILCACCQTSCPSYWWNSDKYLGPAVLMQAFRWVIDSRDEATEQRLCFLQDPWKLYRCHTILNCTNTCPKNLNPARAIIQLKQLLSGAKKKNKPELQTDALFTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9D6</accession>
    <name>A0A6I8W9D6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3 4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3 4" type="primary">LOC117184890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3 4">MV-25-SWS-2005</strain>
            <tissue evidence="2 3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239887.1">
        <property type="nucleotide sequence ID" value="XM_033383996.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239888.1">
        <property type="nucleotide sequence ID" value="XM_033383997.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239889.1">
        <property type="nucleotide sequence ID" value="XM_033383998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9D6"/>
    <dbReference type="KEGG" id="dpo:117184890"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033239887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239889.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13650" checksum="EB48AE4713A479C1" modified="2020-08-12" version="1">MLSRDPEVPHIQKLIEFEASVYKPCKLDLLKKLRYRTVREMRIMLNGEAPSLSWPSSEALGWKRCLPPCPRILKSLDPNPFDGDGFLEVRRSAMRNFKNYFDLGASRAGKVQAGRKDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WAG3</accession>
    <name>A0A6I8WAG3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein gukh isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gukh</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCAR/WAVE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239539.1">
        <property type="nucleotide sequence ID" value="XM_033383648.1"/>
    </dbReference>
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        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WAG3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WAG3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028288">
        <property type="entry name" value="SCAR/WAVE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902">
        <property type="entry name" value="WASP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902:SF23">
        <property type="entry name" value="WISKOTT-ALDRICH SYNDROME PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="421"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="501"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="648"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="718"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="763"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="963"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1087"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1288"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1396"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1510"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1619"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1852"/>
            <end position="1887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="501"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="515"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="533"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="566"/>
            <end position="586"/>
        </location>
    </feature>
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        <location>
            <begin position="650"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="673"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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            <begin position="718"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="728"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="787"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="813"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="844"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="974"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1113"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1510"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1569"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1621"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1872"/>
            <end position="1881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239539.1"/>
        </source>
    </evidence>
    <sequence length="1931" mass="204867" checksum="3ACDBF90EC866A43" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBS9</accession>
    <name>A0A6I8WBS9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30" type="primary">skd</name>
    </gene>
    <organism evidence="13 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15 16" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14 15 16">MV-25-SWS-2005</strain>
            <tissue evidence="14 15 16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240858.1">
        <property type="nucleotide sequence ID" value="XM_033384967.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240859.1">
        <property type="nucleotide sequence ID" value="XM_033384968.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240860.1">
        <property type="nucleotide sequence ID" value="XM_033384969.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240861.1">
        <property type="nucleotide sequence ID" value="XM_033384970.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240862.1">
        <property type="nucleotide sequence ID" value="XM_033384971.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240863.1">
        <property type="nucleotide sequence ID" value="XM_033384972.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240864.1">
        <property type="nucleotide sequence ID" value="XM_033384973.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240865.1">
        <property type="nucleotide sequence ID" value="XM_033384974.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240866.1">
        <property type="nucleotide sequence ID" value="XM_033384975.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240867.1">
        <property type="nucleotide sequence ID" value="XM_033384976.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240868.1">
        <property type="nucleotide sequence ID" value="XM_033384977.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240869.1">
        <property type="nucleotide sequence ID" value="XM_033384978.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240870.1">
        <property type="nucleotide sequence ID" value="XM_033384979.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240871.1">
        <property type="nucleotide sequence ID" value="XM_033384980.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240872.1">
        <property type="nucleotide sequence ID" value="XM_033384981.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240874.1">
        <property type="nucleotide sequence ID" value="XM_033384983.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240875.1">
        <property type="nucleotide sequence ID" value="XM_033384984.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WBS9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
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    <sequence length="2808" mass="299167" checksum="260ECEF39FF35DFA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-05-03" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9C6XP43</accession>
    <name>A0A9C6XP43_DROPS</name>
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        <recommendedName>
            <fullName evidence="4 20">Cytochrome b</fullName>
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        <name evidence="24" type="primary">CYTB</name>
        <name evidence="24" type="ORF">HCS66_mgp05</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="24"/>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2019-12" db="EMBL/GenBank/DDBJ databases">
            <title>Evolutionary conservation of topologically associating domains and their influences on genome synteny and structural variations in Drosophila.</title>
            <authorList>
                <person name="Liao Y."/>
                <person name="Chakraborty M."/>
                <person name="Zhang X."/>
                <person name="Emerson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">MV2-25</strain>
            <tissue evidence="24">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="23 24" key="2">
        <citation type="submission" date="2020-03" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="NCBI Genome Project"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">MV2-25</strain>
            <tissue evidence="24">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">MV2-25</strain>
            <tissue evidence="24">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 20">Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="20">Binds 2 heme groups non-covalently.</text>
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    <comment type="cofactor">
        <cofactor evidence="19">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="19">Binds 2 heme groups non-covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17 20">Belongs to the cytochrome b family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="NC_046603.1"/>
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        <property type="component" value="Mitochondrion MT"/>
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        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Cytochrome_b_N_euk/bac"/>
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        <property type="entry name" value="Di-haem_cyt_TM"/>
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    <dbReference type="PANTHER" id="PTHR19271">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271:SF16">
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    </dbReference>
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        <property type="entry name" value="Cytochrom_B_C"/>
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    </dbReference>
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        <property type="entry name" value="Cytochrome_B"/>
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    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038885">
        <property type="entry name" value="COB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="Transmembrane di-heme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51003">
        <property type="entry name" value="CYTB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 19 20" id="KW-0349">Heme</keyword>
    <keyword evidence="13 19 20" id="KW-0408">Iron</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 20 24" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 20" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 N-terminal region profile" evidence="21">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 C-terminal region profile" evidence="22">
        <location>
            <begin position="211"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>b562</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>b566</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>b562</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>b566</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR038885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="YP_009742604.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="43014" checksum="C0C9AD512B350ED6" modified="2023-05-03" version="1">MNKPLRTSHPLFKIANNALVDLPAPINISSWWNFGSLLGLCLIIQILTGLFLAMHYTADVNMAFYSVNHICRDVNYGWLLRTLHANGASFFFICIYLHVGRGMYYGSYLFTPTWLVGVIILFLVMGTAFMGYVLPWGQMSFWGATVITNLLSAVPYLGIDLVQWVWGGFAVDNATLTRFFTFHFILPFIVLAMTMIHLLFLHQTGSNNPIGLNSNIDKIPFHPYFTFKDIVGFIVMIFLLISLVLINPNLLGDPDNFIPANPLVTPAHIQPEWYFLFAYAILRSIPNKLGGVIALVLSIAILMILPFSNLSKFRGIQFYPLNQILFWTMLVTVILLTWIGARPVEEPYVLLGQILTVIYFLYYLINPLVSKWWDNLLN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-03-23" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>B5DK35</accession>
    <name>DCLK_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Serine/threonine-protein kinase GA29083</fullName>
            <ecNumber>2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Doublecortin-like and CAM kinase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GA29083</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1"/>
    <dbReference type="EMBL" id="CH379061">
        <property type="protein sequence ID" value="EDY70661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DK35"/>
    <dbReference type="SMR" id="B5DK35"/>
    <dbReference type="FunCoup" id="B5DK35">
        <property type="interactions" value="1317"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DK35"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0368464">
        <property type="protein sequence ID" value="FBpp0331227"/>
        <property type="gene ID" value="FBgn0250441"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0376757">
        <property type="protein sequence ID" value="FBpp0337913"/>
        <property type="gene ID" value="FBgn0250441"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902745"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_94_1_1"/>
    <dbReference type="InParanoid" id="B5DK35"/>
    <dbReference type="OMA" id="LMTECKV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250441">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DK35">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16109">
        <property type="entry name" value="DCX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17069">
        <property type="entry name" value="DCX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000866">
        <property type="entry name" value="Serine/threonine-protein kinase GA29083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000017">
        <property type="entry name" value="Serine/threonine-protein kinase GA29083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000021">
        <property type="entry name" value="Serine/threonine-protein kinase GA29083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003533">
        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03607">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00537">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000392569" description="Serine/threonine-protein kinase GA29083">
        <location>
            <begin position="1"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="Doublecortin 1" evidence="4">
        <location>
            <begin position="157"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Doublecortin 2" evidence="4">
        <location>
            <begin position="314"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="484"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="18"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1 5 6">
        <location>
            <position position="605"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <begin position="490"/>
            <end position="498"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P28523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q7PLI7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="EMBL" id="EDY70661.1"/>
        </source>
    </evidence>
    <sequence length="755" mass="82257" checksum="A3C4A040A78EF806" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>B5DK42</accession>
    <accession>A0A6I8UYD2</accession>
    <name>B5DK42_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mitochondrial import inner membrane translocase subunit Tim23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Tim23</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002133266.1">
        <property type="nucleotide sequence ID" value="XM_002133230.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DK42"/>
    <dbReference type="FunCoup" id="B5DK42">
        <property type="interactions" value="724"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DK42"/>
    <dbReference type="GeneID" id="6902754"/>
    <dbReference type="KEGG" id="dpo:6902754"/>
    <dbReference type="eggNOG" id="KOG3324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063935_2_2_1"/>
    <dbReference type="InParanoid" id="B5DK42"/>
    <dbReference type="OMA" id="DNDNIWS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0265247">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DK42">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045238">
        <property type="entry name" value="Tim23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371:SF0">
        <property type="entry name" value="SD19278P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371">
        <property type="entry name" value="TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002133266.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="21804" checksum="CFAB5914DC457135" modified="2008-10-14" version="1">MSDDFLRKPFSLAPAPAVEEATNSNYTSGASKFSSAPVSPYLNYDSRFLQQAQPEFIFPEGANKQRGRFELAFSQIGTSVMIGGGIGGLAGVYNGFKATNALNQTGKLRRTQLINHIMKQGSGTANSLGTLAVLYSACGVLLQYSRGEDDHVNTVIAGSATGLLYKSTAGLRRCALGGAIGLGISSLYCLYLLAQERGTNSSPKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>B5DQI9</accession>
    <accession>A0A6I8W9X4</accession>
    <name>B5DQI9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18 19" type="primary">LOC6900388</name>
    </gene>
    <organism evidence="16 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18 19" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17 18 19">MV-25-SWS-2005</strain>
            <tissue evidence="17 18 19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134781.1">
        <property type="nucleotide sequence ID" value="XM_002134745.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042638.1">
        <property type="nucleotide sequence ID" value="XM_015187152.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240191.1">
        <property type="nucleotide sequence ID" value="XM_033384300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DQI9"/>
    <dbReference type="STRING" id="46245.B5DQI9"/>
    <dbReference type="GeneID" id="6900388"/>
    <dbReference type="KEGG" id="dpo:6900388"/>
    <dbReference type="eggNOG" id="KOG0070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_9_3_1"/>
    <dbReference type="OMA" id="MRREWFV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245013">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 15" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002134781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015042638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033240191.1"/>
        </source>
    </evidence>
    <sequence length="181" mass="20598" checksum="79E639FFA3B1C8BF" modified="2008-10-14" version="1">MGNAFANLFKGLFGKKQINVLMVGLDNAGKTTILYKLKLGEVITGIPTIGFNVETVEYKNITFTVWDVGGQDRIRPLWRQYFLHMTQFIIFVVDSNDRERIEEARDELRRMMVEDELRVLLIFANKEDLPNAMSAAEVADKLGLHSLTNRNCHVQSTCATNGDGLYEGLEWLSNQLKNANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>B5DQR8</accession>
    <accession>A0A6I8V0C8</accession>
    <name>B5DQR8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Y+L amino acid transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">mnd</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
            <tissue evidence="11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134757.1">
        <property type="nucleotide sequence ID" value="XM_002134721.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241439.1">
        <property type="nucleotide sequence ID" value="XM_033385548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DQR8"/>
    <dbReference type="STRING" id="46245.B5DQR8"/>
    <dbReference type="GeneID" id="6900362"/>
    <dbReference type="KEGG" id="dpo:6900362"/>
    <dbReference type="eggNOG" id="KOG1287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_3_0_1"/>
    <dbReference type="OMA" id="PFWPSCE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245021">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000003">
        <property type="entry name" value="Y+L amino acid transporter 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF240">
        <property type="entry name" value="LD25378P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002134757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033241439.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="55103" checksum="B15FFE537F8FEE94" modified="2008-10-14" version="1">MARIQASDSLIPRQIFEMPPAEQPSRDRENQSSADSDSQGSGVKLKKQIGLLDGVAIIVGVIVGSGIFVSPKGVLKLSGSIGQSLIVWVLCGVLSMVGALCYAELGTMIPKSGGDYAYIGTAFGPLPAFLYLWVALLILVPTGNAITALTFAQYLLKPFWPSCEAPAEAVQLLAAAMICVLTVINCYNVKWVTRVTDIFTGTKVLALLVIVGAGVWYLADGNTEHWDKPFSGGNLSPGYIALAFYNGLFSYSGWNYLNFVTEELKDPYRNLPKAICISMPVVTIIYLVTNVAYFSVLSTDEILSSDAVAVTFGDKMLDYMSWVMPFAVACSTFGSLNGAIFASSRLFFVGARNGHLPAAISLINVNCLTPVPSLIFLGVLTLLLLFIKDTYVLINYVSYVEALFTLISVSGLLWLRYKQPKTERPIRVNLALPIIYLIVCLFLVISSCSQSPYEVGIGTIIILSGIPVYYLTIHHPVKWLADTSQSINLWCSKFFICMPNQEKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I7LTN7</accession>
    <accession>A0A6I8UB43</accession>
    <name>I7LTN7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RNA-binding protein lark</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">lark</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001352689.1">
        <property type="nucleotide sequence ID" value="XM_001352653.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7LTN7"/>
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        <property type="interactions" value="1259"/>
    </dbReference>
    <dbReference type="STRING" id="46245.I7LTN7"/>
    <dbReference type="GeneID" id="4812437"/>
    <dbReference type="KEGG" id="dpo:4812437"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045263_1_0_1"/>
    <dbReference type="InParanoid" id="I7LTN7"/>
    <dbReference type="OMA" id="GYDSMFS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081181">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12343">
        <property type="entry name" value="RRM1_2_CoAA_like"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="RNA-binding protein 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding protein lark"/>
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    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
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    </dbReference>
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        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="254"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="320"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001352689.1"/>
        </source>
    </evidence>
    <sequence length="352" mass="39891" checksum="D1CB37E81C2B351C" modified="2012-10-03" version="1">MPGAGTFKLFIGNLDEKTQATELRALFEKYGTVVECDVVKNYGFVHMETEQQGRDAIQNLNGYVLNDNAIKVEAAKSRRAPNTPTTKIFVGNLTDKTRAPEVRELFQKYGTVVECDIVRNYGFVHLDCVGDVQDAIKELNGRVVDGQPLKVQVSTSRVRPKPGMGDPEQCYRCGRSGHWSKECPRLYGSTGGGREPPSPLSAGGYRDRMYGRDPYPPPPPPPPFLRDRIMDGFRDYDYYDRRFEDSRDLYERRYQSSRMRDFPPPPISRREPMPLPPPLSGSLRSCSVTRGYDTMFSRRSPPPPRSSNGMSRYGSPTPHGYEDFSRDSFDDRMISSRGMRGPSPPGRRYAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q29A29</accession>
    <accession>A0A6I8UNJ7</accession>
    <name>Q29A29_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Programmed cell death protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Ccm3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PDCD10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358383.1">
        <property type="nucleotide sequence ID" value="XM_001358346.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29A29"/>
    <dbReference type="SMR" id="Q29A29"/>
    <dbReference type="FunCoup" id="Q29A29">
        <property type="interactions" value="1302"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29A29"/>
    <dbReference type="GeneID" id="4801261"/>
    <dbReference type="KEGG" id="dpo:4801261"/>
    <dbReference type="eggNOG" id="KOG4025">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083906_1_0_1"/>
    <dbReference type="InParanoid" id="Q29A29"/>
    <dbReference type="OMA" id="YQCLYSG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078639">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090443">
        <property type="term" value="C:FAR/SIN/STRIPAK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903358">
        <property type="term" value="P:regulation of Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1950:FF:000001">
        <property type="entry name" value="Cerebral cavernous malformation 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009652">
        <property type="entry name" value="PDCD10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053750">
        <property type="entry name" value="PDCD10_Homolog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048288">
        <property type="entry name" value="PDCD10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13250:SF1">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13250">
        <property type="entry name" value="TF-1 CELL APOPTOSIS RELATED PROTEIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06840">
        <property type="entry name" value="PDC10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20929">
        <property type="entry name" value="PDCD10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 10 dimerisation" evidence="7">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001358383.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="23864" checksum="D29E8FB19BBD4217" modified="2006-04-04" version="1">MTMGEREPTSSLVLPVILRPIFSQLERRDVGAAQSLRSAILKSEQNNPGFCYDLVATIVRRADLNVNLNEAVLRLQGNITEADLNEYRLTRTEEPFQELNRKSVALKVILSRIPDEINDRKTFLETIKEIASAIKKLLDVVNEIGSFIPGVTGKQAVEQRKKEFVKYSKKFSTTLKEYFKEGQPNAVFISALFLIRQTNQIMLTVKSKCE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-10-19" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29BT3</accession>
    <name>GATA_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial</fullName>
            <shortName evidence="1">Glu-AdT subunit A</shortName>
            <ecNumber evidence="1">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">GatA</name>
        <name type="ORF">GA19290</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="1"/>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL26914.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29BT3"/>
    <dbReference type="SMR" id="Q29BT3"/>
    <dbReference type="FunCoup" id="Q29BT3">
        <property type="interactions" value="799"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29BT3"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0284775">
        <property type="protein sequence ID" value="FBpp0283213"/>
        <property type="gene ID" value="FBgn0079287"/>
    </dbReference>
    <dbReference type="GeneID" id="4800514"/>
    <dbReference type="KEGG" id="dpo:4800514"/>
    <dbReference type="CTD" id="42283"/>
    <dbReference type="eggNOG" id="KOG1211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_7_6_1"/>
    <dbReference type="InParanoid" id="Q29BT3"/>
    <dbReference type="OMA" id="QPASYCG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079287">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000413337" description="Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial">
        <location>
            <begin position="1"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03150"/>
        </source>
    </evidence>
    <sequence length="507" mass="55547" checksum="03C81440F1640C33" modified="2008-10-14" version="2">MRRHLQWSIKQLTSSYADGQLSPRRVTEQALEDSVNLKTLNAFVRLTPEEARQQAQQSEQRYQKKQPSSGLDGVTIAIKDNFCTENVHTTCASRMLQDFVPPYDATVCSRLRQAGAVLLGKTNMDQFAMGAGTVDSIYGPTKNIWSEDLTQNNWRIAGGSSGGSASAVAAGLCFAGIGSDTGGSTRNPASYCGVVGLKPTYGLVSRHGLIPLVNSMDVPGILARSVSDCVEVLNAVAGPDGKDSTTIRQPFTKLQLPEVDGLDLQTVRIGIPKEYHCNGLSAEVLETWTKVADLLECAGASVQQVSLPNTAASIFVYSILNQCEVASNMARYDGIEFGHRAADERSTEQLYAQSRAEGFNDVVKTRILTGNFLLLRKNYDHYFEKALRVRRLIAEDFLKVFEAPSKEDRVDILLTPTTLTEAPLYKDFSSLSNRDQCAVQDFCTQPANMAGIPAVSIPIRLSRSGLPLSLQLMSRSLNEQLLLSVARWIEAQVAFDSLDHRLEQKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DV4</accession>
    <accession>A0A6I8UBB2</accession>
    <name>Q29DV4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Ptp61F</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001352809.2">
        <property type="nucleotide sequence ID" value="XM_001352773.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DV4"/>
    <dbReference type="FunCoup" id="Q29DV4">
        <property type="interactions" value="1965"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DV4"/>
    <dbReference type="GeneID" id="4812118"/>
    <dbReference type="KEGG" id="dpo:4812118"/>
    <dbReference type="eggNOG" id="KOG0789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001645_9_0_1"/>
    <dbReference type="InParanoid" id="Q29DV4"/>
    <dbReference type="OMA" id="PVRAFNE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081581">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DV4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070373">
        <property type="term" value="P:negative regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046426">
        <property type="term" value="P:negative regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14545">
        <property type="entry name" value="PTPc-N1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051985">
        <property type="entry name" value="NR_tyrosine_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46047">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 61F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46047:SF3">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 61F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="10">
        <location>
            <begin position="41"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="11">
        <location>
            <begin position="219"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="406"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="423"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="464"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001352809.2"/>
        </source>
    </evidence>
    <sequence length="570" mass="64050" checksum="6136289BAD374795" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EH5</accession>
    <accession>A0A6I8VLB7</accession>
    <name>Q29EH5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Multiple inositol polyphosphate phosphatase 1</fullName>
            <ecNumber evidence="4">3.1.3.62</ecNumber>
            <ecNumber evidence="3">3.1.3.80</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2,3-bisphosphoglycerate 3-phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Mipp1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R)-2,3-bisphosphoglycerate + H2O = (2R)-2-phosphoglycerate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27381"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="3.1.3.80"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:27382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,2,5,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77115"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:77116"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol 1,2,5,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,2,6-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="ChEBI" id="CHEBI:195537"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:77120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16989"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:16990"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family. MINPP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.62" evidence="4"/>
    <dbReference type="EC" id="3.1.3.80" evidence="3"/>
    <dbReference type="RefSeq" id="XP_015043705.1">
        <property type="nucleotide sequence ID" value="XM_015188219.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EH5"/>
    <dbReference type="FunCoup" id="Q29EH5">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EH5"/>
    <dbReference type="GeneID" id="4811883"/>
    <dbReference type="KEGG" id="dpo:4811883"/>
    <dbReference type="eggNOG" id="KOG1382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029165_0_0_1"/>
    <dbReference type="InParanoid" id="Q29EH5"/>
    <dbReference type="OMA" id="SLHSPWC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077983">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EH5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034417">
        <property type="term" value="F:bisphosphoglycerate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052745">
        <property type="term" value="F:inositol phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000014">
        <property type="entry name" value="Multiple inositol polyphosphate phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF8">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015043705.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="53719" checksum="6A1CC00A8FAED9F6" modified="2006-04-04" version="1">MRFLLLLLVPLVAPLLVDGQEDYCFGKDSDRPQTAQFASKTAYQIVKSSNIDKQYLVPGCEAKKMWVFHRHGTRLPKSGTIKKASRLIELRDQIVKNYREAKTKPDTNALCTEDLIAIQLWKGNSSITPDMDEFLTSQGYDDLRGTAKLYQRYYPTVLPKDYNDTYYQFRHTDTQRTTESFKAFAEGLFGTGNAAHPVDIPKDDLLLRPYDYCQSYKELNYKGEGSEYHKYTQSALWNSTLADISTRLGYLYTLDQADILLMYDTCRYEQAWQVDRTSVWCGAFLPEQVTVFEYAEDLKYYYGSGYGFEEDTARLNCRLVQDMLTHLSNPVSPHVIAHFGHTTGLQTLITALGIRKDDIKLRADNYNSLTNRRWKTSILGPFAANFVAVKYDCPAALDKEKAVFFLNQDAVQLDWCSVGLCNWSEVLQRYKTLADANCDEYFCRSGASSLAGGALSSLLASFLLAAVVYLMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JY2</accession>
    <accession>A0A6I8UHV9</accession>
    <name>Q29JY2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Wee1-like protein kinase</fullName>
            <ecNumber evidence="10">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Wee1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001355770.1">
        <property type="nucleotide sequence ID" value="XM_001355734.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JY2"/>
    <dbReference type="SMR" id="Q29JY2"/>
    <dbReference type="FunCoup" id="Q29JY2">
        <property type="interactions" value="1459"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JY2"/>
    <dbReference type="GeneID" id="4816185"/>
    <dbReference type="KEGG" id="dpo:4816185"/>
    <dbReference type="eggNOG" id="KOG0601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_25_1_1"/>
    <dbReference type="InParanoid" id="Q29JY2"/>
    <dbReference type="OMA" id="TIFNHPV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078225">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000115">
        <property type="entry name" value="Wee1-like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017164">
        <property type="entry name" value="Wee1-like_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF185">
        <property type="entry name" value="WEE1-LIKE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037281">
        <property type="entry name" value="Wee1-like_protein_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="221"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037281-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001355770.1"/>
        </source>
    </evidence>
    <sequence length="585" mass="66321" checksum="410DE82EB30ED3EC" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KL8</accession>
    <name>DAAF1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Dynein axonemal assembly factor 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Defective transmitter-recycling protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Leucine-rich repeat-containing protein 50 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">dtr</name>
        <name type="ORF">GA16341</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium-specific protein required for cilia structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNAAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379061">
        <property type="protein sequence ID" value="EAL33156.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KL8"/>
    <dbReference type="SMR" id="Q29KL8"/>
    <dbReference type="STRING" id="46245.Q29KL8"/>
    <dbReference type="eggNOG" id="ENOG502QQFE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001523_0_0_1"/>
    <dbReference type="InParanoid" id="Q29KL8"/>
    <dbReference type="OMA" id="WKREGYE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000166">
        <property type="entry name" value="Dynein assembly factor 1, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000331">
        <property type="entry name" value="Dynein assembly factor 1, axonemal homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0966">Cell projection</keyword>
    <keyword id="KW-0969">Cilium</keyword>
    <keyword id="KW-0433">Leucine-rich repeat</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000363939" description="Dynein axonemal assembly factor 1 homolog">
        <location>
            <begin position="1"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 2">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 3">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 4">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 5">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 6">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT">
        <location>
            <begin position="189"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="245"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="859"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="913"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1073"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1101"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1150"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1305"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1358"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1484"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1529"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="339"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="928"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1078"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1103"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1166"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1321"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1398"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1484"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1529"/>
            <end position="1540"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1582" mass="180080" checksum="7211B273760460EC" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29L61</accession>
    <accession>A0A6I8UIE6</accession>
    <name>Q29L61_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ubiquitin conjugation factor E4 B</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase E4 B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquitin fusion degradation protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">Ube4B</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21">MV-25-SWS-2005</strain>
            <tissue evidence="21">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001355904.3">
        <property type="nucleotide sequence ID" value="XM_001355868.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29L61"/>
    <dbReference type="SMR" id="Q29L61"/>
    <dbReference type="FunCoup" id="Q29L61">
        <property type="interactions" value="3087"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29L61"/>
    <dbReference type="GeneID" id="4816545"/>
    <dbReference type="KEGG" id="dpo:4816545"/>
    <dbReference type="eggNOG" id="KOG2042">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003224_2_0_1"/>
    <dbReference type="InParanoid" id="Q29L61"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082116">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29L61">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16658">
        <property type="entry name" value="RING-Ubox_UBE4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000060">
        <property type="entry name" value="ubiquitin conjugation factor E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="19">
        <location>
            <begin position="1132"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="147"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="679"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_001355904.3"/>
        </source>
    </evidence>
    <sequence length="1209" mass="138133" checksum="078A347701E08563" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MM1</accession>
    <accession>A0A6I8UJQ3</accession>
    <name>Q29MM1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cytidine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4817053</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine + H2O + H(+) = uridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001356608.2">
        <property type="nucleotide sequence ID" value="XM_001356572.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MM1"/>
    <dbReference type="SMR" id="Q29MM1"/>
    <dbReference type="FunCoup" id="Q29MM1">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29MM1"/>
    <dbReference type="GeneID" id="4817053"/>
    <dbReference type="KEGG" id="dpo:4817053"/>
    <dbReference type="eggNOG" id="KOG0833">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097262_2_0_1"/>
    <dbReference type="InParanoid" id="Q29MM1"/>
    <dbReference type="OMA" id="DFPIYIA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080999">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055086">
        <property type="term" value="P:nucleobase-containing small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072527">
        <property type="term" value="P:pyrimidine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000008">
        <property type="entry name" value="Cytidine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050202">
        <property type="entry name" value="Cyt/Deoxycyt_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004064">
        <property type="entry name" value="PRK05578.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644:SF2">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001356608.2"/>
        </source>
    </evidence>
    <sequence length="227" mass="25644" checksum="747348728F98C78B" modified="2008-10-14" version="2">MSDKRKLLKKYMKCLDEMKSELKRRQLKKTLKTHIKALNDLECELKRREGKHTGGKRGRVCDSSNLPHPQILDEYTTPYCRLREEARELLEAAVVARKEAYVPYSKFPVGASFRSKCGYVYSGCNIENVAFTPGCCAERTALAKGVSEGQLTYTAGAVVAYHPKGFTTPCGVCRQFLREFACRDFPIYIANAPCPDREGAIPAIRDEDEVLVTSVYRLLPHSFSTFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29MV3</accession>
    <accession>A0A6I8UJL7</accession>
    <name>Q29MV3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Nepl9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Nepl9</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356526.1">
        <property type="nucleotide sequence ID" value="XM_001356490.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29MV3"/>
    <dbReference type="FunCoup" id="Q29MV3">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="GeneID" id="4817118"/>
    <dbReference type="KEGG" id="dpo:4817118"/>
    <dbReference type="eggNOG" id="KOG3624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_421672_0_0_1"/>
    <dbReference type="InParanoid" id="Q29MV3"/>
    <dbReference type="OMA" id="HAEYWQW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072218">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29MV3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF222">
        <property type="entry name" value="IP12942P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356526.1"/>
        </source>
    </evidence>
    <sequence length="645" mass="76190" checksum="98D18B00AFBAB5E5" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29P47</accession>
    <accession>A0A6I8ULN8</accession>
    <name>Q29P47_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger protein Noc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">noc</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Elbow/Noc family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357377.1">
        <property type="nucleotide sequence ID" value="XM_001357341.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29P47"/>
    <dbReference type="FunCoup" id="Q29P47">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="GeneID" id="4817885"/>
    <dbReference type="KEGG" id="dpo:4817885"/>
    <dbReference type="eggNOG" id="ENOG502QUYV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035082_0_0_1"/>
    <dbReference type="InParanoid" id="Q29P47"/>
    <dbReference type="OMA" id="LMYPSPH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078226">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29P47">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051520">
        <property type="entry name" value="Elbow/Noc_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522:SF5">
        <property type="entry name" value="ZINC FINGER PROTEIN NOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522">
        <property type="entry name" value="ZINC-FINGER PROTEIN NOLZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001357377.1"/>
        </source>
    </evidence>
    <sequence length="537" mass="54384" checksum="EC07DD80A0890CA2" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PD0</accession>
    <accession>A0A6I8ULD2</accession>
    <name>Q29PD0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cytochrome c oxidase subunit 5B, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COX5B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357294.1">
        <property type="nucleotide sequence ID" value="XM_001357258.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PD0"/>
    <dbReference type="FunCoup" id="Q29PD0">
        <property type="interactions" value="1218"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29PD0"/>
    <dbReference type="GeneID" id="4818143"/>
    <dbReference type="KEGG" id="dpo:4818143"/>
    <dbReference type="eggNOG" id="KOG3352">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127178_1_0_1"/>
    <dbReference type="InParanoid" id="Q29PD0"/>
    <dbReference type="OMA" id="NDDPFGM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070765">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00924">
        <property type="entry name" value="Cyt_c_Oxidase_Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.11.10:FF:000004">
        <property type="entry name" value="Cytochrome c oxidase subunit 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.11.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002124">
        <property type="entry name" value="Cyt_c_oxidase_su5b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036972">
        <property type="entry name" value="Cyt_c_oxidase_su5b_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01215">
        <property type="entry name" value="COX5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51359">
        <property type="entry name" value="COX5B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602124-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357294.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="13551" checksum="B7064EC9E384C01B" modified="2006-04-04" version="1">MASICGRMALRAAARQNVTYAPVRFCKMMNDPLEHATGIEKRELLAKAAGNENPFDMKVFKRGAGTKENPNLIPSAFDARIVGCICEEDQTYVQWMWLQKGNQKRCECGHWFKLVEKAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NQY4</accession>
    <accession>A0A6I8V7Q2</accession>
    <name>A0A0R3NQY4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gustatory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4817820</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035527.1">
        <property type="nucleotide sequence ID" value="XM_015180041.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NQY4"/>
    <dbReference type="FunCoup" id="A0A0R3NQY4">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3NQY4"/>
    <dbReference type="GeneID" id="4817820"/>
    <dbReference type="InParanoid" id="A0A0R3NQY4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NQY4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008049">
        <property type="term" value="P:male courtship behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013604">
        <property type="entry name" value="7TM_chemorcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143:SF104">
        <property type="entry name" value="GUSTATORY RECEPTOR 8A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21143">
        <property type="entry name" value="INVERTEBRATE GUSTATORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08395">
        <property type="entry name" value="7tm_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015035527.1"/>
        </source>
    </evidence>
    <sequence length="464" mass="53164" checksum="C15D3598410F076B" modified="2016-01-20" version="1">MEAGKDTDTVEIEVESGLCQPLRRRVRRFLSAKQLYECLRPVFHVTYWHGLTSFYISSDSATGRKDIKKTIFGFVNGIIHITLYAICYTLTIFNNCESVASYFFRSRITYFGDMMQIVSGFIGVTVIYLTSIIPNHRLERCLEKFHTMDMQLQTVGIKIMYSKVLRFSYMILFSMFMVNICFTCGTFSVLYSSLVAPSMALHFTFLIQHTVISIAIAVFSCFTYLVEMRLVMVNKVLKNLSHQWDSHSLKGVVQKQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLMNKTKSPEVKEKLQQFSMQLLHLKINFTAAGLFNIDRTLYFTISGALTTYLIILLQFTSNSPHNSNGNYYSCCETFNNITNHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NY79</accession>
    <accession>A0A6I8V8V3</accession>
    <name>A0A0R3NY79_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GTPase-activating protein skywalker isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sky</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015036245.1">
        <property type="nucleotide sequence ID" value="XM_015180759.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NY79"/>
    <dbReference type="SMR" id="A0A0R3NY79"/>
    <dbReference type="FunCoup" id="A0A0R3NY79">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3NY79"/>
    <dbReference type="GeneID" id="6903078"/>
    <dbReference type="InParanoid" id="A0A0R3NY79"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247397">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NY79">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000035">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF122">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN SKYWALKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015036245.1"/>
        </source>
    </evidence>
    <sequence length="612" mass="70056" checksum="C4E034B54F2BE051" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P1U2</accession>
    <accession>A0A6I8VMB7</accession>
    <name>A0A0R3P1U2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="18">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">PMCA</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="18"/>
    <dbReference type="RefSeq" id="XP_015044270.1">
        <property type="nucleotide sequence ID" value="XM_015188784.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P1U2"/>
    <dbReference type="SMR" id="A0A0R3P1U2"/>
    <dbReference type="FunCoup" id="A0A0R3P1U2">
        <property type="interactions" value="787"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P1U2"/>
    <dbReference type="GeneID" id="6899891"/>
    <dbReference type="InParanoid" id="A0A0R3P1U2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243914">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P1U2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Calcium-transporting ATPase"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
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    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
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    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
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        <property type="entry name" value="ATP_Ca_trans_C"/>
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        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
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        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
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        <property type="entry name" value="Haloacid_Dehalogenase"/>
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        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
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    </dbReference>
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        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10 18" id="KW-0106">Calcium</keyword>
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    <keyword evidence="13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 18" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
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        <location>
            <begin position="1005"/>
            <end position="1025"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
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            <end position="1055"/>
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            <begin position="307"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="333"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="RuleBase" id="RU361146"/>
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        <source>
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        <source>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBX4</accession>
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        <name evidence="4" type="primary">LOC4812681</name>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
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        </source>
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        <name evidence="3" type="primary">LOC4812707</name>
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            <begin position="1"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5026018810" evidence="1">
        <location>
            <begin position="26"/>
            <end position="156"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001353087.3"/>
        </source>
    </evidence>
    <sequence length="156" mass="17130" checksum="E18C563E77CE9A0F" modified="2020-08-12" version="1">MQQYQFLRTLCLFLMISSMVVYIQSECNKCQSNNNVACVNKTHYRFCLDNVEPGAILPCNDGEVCTSLAKICVPEGAYEPSCQDSEATTPNECPSCTASSLFVCTSRTTFQQCNGTTLTDRVIKCNEGKFCSMASGKYCVGECELTGDIECDRDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCY9</accession>
    <name>A0A6I8UCY9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">LIM/homeobox protein Awh isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Awh</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353549.4">
        <property type="nucleotide sequence ID" value="XM_001353513.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCY9"/>
    <dbReference type="SMR" id="A0A6I8UCY9"/>
    <dbReference type="FunCoup" id="A0A6I8UCY9">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812758"/>
    <dbReference type="InParanoid" id="A0A6I8UCY9"/>
    <dbReference type="OMA" id="DFGRHIN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCY9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007444">
        <property type="term" value="P:imaginal disc development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09373">
        <property type="entry name" value="LIM1_AWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09379">
        <property type="entry name" value="LIM2_AWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000023">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000006">
        <property type="entry name" value="LIM homeobox transcription factor 1-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000027">
        <property type="entry name" value="LIM/homeobox protein Lhx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF127">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN AWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="68"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="146"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="148"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001353549.4"/>
        </source>
    </evidence>
    <sequence length="275" mass="31054" checksum="8AD0BC0F2BE1DBE6" modified="2020-08-12" version="1">MKTELRSCAACGEPISDRFFLEVGGCSWHAHCLRCCMCMCPLDRQQSCFIRERQVYCKADYSKNFGAKCSKCCRGISASDWVRRARDLVFHLACFACDQCGRQLSTGEQFALMDDRVLCKAHYLETVEGGTTSSDEGCDGDGYHKSKTKRVRTTFTEEQLQVLQANFQIDSNPDGQDLERIASVTGLSKRVTQVWFQNSRARQKKHIHAGKNKIREPEGSSFARHINLQLTYSFQNNAQNPMHMNGSKAGLYPTHESSMDEMSQDSSVHCMPSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGL2</accession>
    <name>A0A6I8UGL2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Glos</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Glos</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355163.3">
        <property type="nucleotide sequence ID" value="XM_001355127.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGL2"/>
    <dbReference type="FunCoup" id="A0A6I8UGL2">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815270"/>
    <dbReference type="InParanoid" id="A0A6I8UGL2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355163.3"/>
        </source>
    </evidence>
    <sequence length="914" mass="104999" checksum="422C925003FF4011" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIT4</accession>
    <name>A0A6I8UIT4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tubulin gamma chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gammaTub23C</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with Ote.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356148.3">
        <property type="nucleotide sequence ID" value="XM_001356112.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIT4"/>
    <dbReference type="FunCoup" id="A0A6I8UIT4">
        <property type="interactions" value="1064"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816635"/>
    <dbReference type="InParanoid" id="A0A6I8UIT4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIT4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02188">
        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000004">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000003">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000010">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01164">
        <property type="entry name" value="GAMMATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="10">
        <location>
            <begin position="48"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="11">
        <location>
            <begin position="249"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001356148.3"/>
        </source>
    </evidence>
    <sequence length="471" mass="53061" checksum="4BDB75B70ABA7166" modified="2020-08-12" version="1">MPSEIITLQLGQCGNQIGFEFWKRLCMEHGISPSGVLEDFATEGMDRKDVFFYQADDDHYIPRAVLLDLEPRVINTIMGSSYAKLYNPENVYLSKHGGGAGNNWASGYSQGEKLQEEIFDIIDREADGSDSLEGFILCHSIAGGTGSGMGSFIMERLADRYPKKLIQTFSVFPNQDEISDVVVQPYNSLLTLRRLTSAADSVVVLDNTALNRIACDRLHIQNPSFSQINNLVSTIMSVSTTTLRYPSYMNNNLIGLMASLIPTPQLHFLMTGYTPLTSASDVNNQQTVNIRKTTVLDVMRRLLQPKNMMVSTGPDKTNHHCYISILNIIQGEVDPSQVHKSLQRIRDRKMAQFIPWGPTSIQVALSRSSPYVQSNHRVSGLMLANHTSINSLFERALNQYDKLRKRGAFLDQFRREDIFKDDLNELDESRETVDSLVQEYEAATRSDYMHYSFKRAQENSKSEDAKSGGDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJD9</accession>
    <name>A0A6I8UJD9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein aristaless</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">al</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356444.2">
        <property type="nucleotide sequence ID" value="XM_001356408.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJD9"/>
    <dbReference type="SMR" id="A0A6I8UJD9"/>
    <dbReference type="FunCoup" id="A0A6I8UJD9">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817187"/>
    <dbReference type="InParanoid" id="A0A6I8UJD9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000102">
        <property type="entry name" value="Aristaless related homeobox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF579">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="81"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="10">
        <location>
            <begin position="367"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="83"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="249"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356444.2"/>
        </source>
    </evidence>
    <sequence length="397" mass="42698" checksum="6BCB1C9CBBD47A6E" modified="2020-08-12" version="1">MGISEEIKLEELPQEAKLGHPDGVVVDRGSVLVSSASAATAGASLTVSVSGGAPSGASGGTNSPISDANSDCEADEYAPKRKQRRYRTTFTSFQLEELEKAFSRTHYPDVFTREELAMKIGLTEARIQVWFQNRRAKWRKQEKVGPQSHPYNPYLPSGAASMQTVVGAALPPNPFTHLGFQLRKPFDAQHAANLAAFRYPHLSAAPMIPSGYFNQFQRAPPHMLPPGMAGMYSPSSSFQSLLANMTAVPRGPHLPPMGGKPMLVGSPDLHSPNHLLASPPTSPASGASQHQHQHQPPPQPPPPHVQPSQLSPQQLVGIALTHQAPCGSPTQTAPVALTLAHSPQRQMPPAPLPPPRSSTPPEDRRTSSIAALRLKAREHELKLELLRQNGHGNDVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJI1</accession>
    <name>A0A6I8UJI1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4816820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356507.2">
        <property type="nucleotide sequence ID" value="XM_001356471.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJI1"/>
    <dbReference type="FunCoup" id="A0A6I8UJI1">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816820"/>
    <dbReference type="InParanoid" id="A0A6I8UJI1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJI1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356507.2"/>
        </source>
    </evidence>
    <sequence length="240" mass="26043" checksum="FDC54A8E75A9D900" modified="2020-08-12" version="1">MELRSQSFHALVNLLKDAGVYEEGMAYDEVKQLAQAMQLSTMEQIEENDEYLQFERARSASIESYSSLELHQSTMLPSVLMAEPVAGDVAIDVSQRDCDSPPCEPVRMTVRAIVHHPLDWSPSAEHQPLPKRRADGNSNAIGSEEKRFRTDNNNNNNNNQAQQQQPAAAEVDVTPASLASEDSGVSWDALSSVNSSAYDSSLVPSIGEMPSGLMVSTSSAAVSGSSRSNIHNSDSEINSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK05</accession>
    <name>A0A6I8UK05_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">WW domain-binding protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4817115</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356747.4">
        <property type="nucleotide sequence ID" value="XM_001356711.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK05"/>
    <dbReference type="FunCoup" id="A0A6I8UK05">
        <property type="interactions" value="977"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817115"/>
    <dbReference type="InParanoid" id="A0A6I8UK05"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK05">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040023">
        <property type="entry name" value="WBP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173:SF10">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="8">
        <location>
            <begin position="11"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="7">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="99"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356747.4"/>
        </source>
    </evidence>
    <sequence length="341" mass="38951" checksum="82E16F3C0859CE1C" modified="2020-08-12" version="1">MTEFWKSNERKYCDFCKCWLSDNKASIAFHEDGKRHKLNVAKRITDISRSSEKSERERLKMDADIRKMEDAAMRSYAQDIHGRADMTARSINTVMSAVAASGSSGSRGGTNAPGRGRQIDPMRLEGYSDEEEDNRRVAPGKVTADAAVPDASLWVEGKSDEGHTYYWNVKTNESVWKPPKEGYLSYEEYERINQLAINQQELSQEQESLRFRANADEEVARVNRERLKAFRKPETAKEKKQKEEKRLEFKTEEEAATKEIGQWQTVEVKAPEEPIDWELPQSDYYVPPPVVSTSSEPEPPVKRFKEKTISGLDGEAAASVPSTFKKRKFANKGNARKRLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKJ1</accession>
    <name>A0A6I8UKJ1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyoxylate reductase/hydroxypyruvate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4817449</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356851.3">
        <property type="nucleotide sequence ID" value="XM_001356815.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKJ1"/>
    <dbReference type="FunCoup" id="A0A6I8UKJ1">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817449"/>
    <dbReference type="InParanoid" id="A0A6I8UKJ1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKJ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000026">
        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF119">
        <property type="entry name" value="FI03731P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="45"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="150"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001356851.3"/>
        </source>
    </evidence>
    <sequence length="362" mass="39393" checksum="A7F23AA07ABAC694" modified="2020-08-12" version="1">MLSRFIGVSATIISSRSGSILSRSLYKSGGRNHTQTMSTGKAFKVLITHPEVPQEGIDLLKENCEIIQVQSLPVDRAELLQKIRGVDGIFWGGHEALNAEALDAAGPQLKSISTMSAGIDYVDVAEVKKRKIPLGHTPTVLNTAVADLAVGLLIAAGRRFHEGRKKIETNNWENYHLNWMLGQDIRDSTVGFYGFGGIGQAIAKRLSGFDIDQVLYTTRRRVDKEIEKELNAKKVDFDTLLAQSDFVVIASPLTAATQGVFNATAFNKMKETAVLVNIGRGKIVNQDDLYEALKSNKIFSAGLDVTDPEPLSAKDKLLSLDNVVVLPHIGSATKRTRAEMATIAAHNVLRGLVGEPMLSPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULG8</accession>
    <name>A0A6I8ULG8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein aubergine-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC4817993</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoplasmic ribonucleoprotein granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the argonaute family. Piwi subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357395.3">
        <property type="nucleotide sequence ID" value="XM_001357359.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULG8"/>
    <dbReference type="FunCoup" id="A0A6I8ULG8">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817993"/>
    <dbReference type="InParanoid" id="A0A6I8ULG8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULG8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034584">
        <property type="term" value="F:piRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009994">
        <property type="term" value="P:oocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007279">
        <property type="term" value="P:pole cell formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140965">
        <property type="term" value="P:secondary piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141009">
        <property type="term" value="P:transposable element silencing by piRNA-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02845">
        <property type="entry name" value="PAZ_piwi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000404">
        <property type="entry name" value="Argonaut-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000014">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000003">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23278">
        <property type="entry name" value="Piwi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="PAZ" evidence="13">
        <location>
            <begin position="267"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="14">
        <location>
            <begin position="541"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001357395.3"/>
        </source>
    </evidence>
    <sequence length="852" mass="97588" checksum="4B287B63FA7BD9F9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMG1</accession>
    <name>A0A6I8UMG1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Protein phosphatase 1B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">alph</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357754.3">
        <property type="nucleotide sequence ID" value="XM_001357717.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMG1"/>
    <dbReference type="FunCoup" id="A0A6I8UMG1">
        <property type="interactions" value="2453"/>
    </dbReference>
    <dbReference type="GeneID" id="4800487"/>
    <dbReference type="KEGG" id="dpo:4800487"/>
    <dbReference type="InParanoid" id="A0A6I8UMG1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075147">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UMG1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.430">
        <property type="entry name" value="Phosphatase 2C, C-terminal domain suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012911">
        <property type="entry name" value="PP2C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036580">
        <property type="entry name" value="PP2C_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07830">
        <property type="entry name" value="PP2C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81601">
        <property type="entry name" value="Protein serine/threonine phosphatase 2C, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 16" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="17">
        <location>
            <begin position="23"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001357754.3"/>
        </source>
    </evidence>
    <sequence length="367" mass="40925" checksum="25756CD576CF3387" modified="2020-08-12" version="1">MGGFLDKPKTAKHNDHGEGNKLLFGVSSMQGWRCEMEDAYYARVGLDNALNDWSFFAVFDGHAGCKVSEHCAKHLLDSIVHTDEFISGDHVKGIRTGFLRIDEVMRDLPEFTKDSKCGGTTAVCAFVSSTQVYIANCGDSRAVLCRQGVPVFATQDHKPILPEEKERIHNAGGSVMIKRVNGTLAVSRALGDYDFKNVKEKGQCEQLVSPEPEIFCQSRQDTDEFLVLACDGIWDVMTNEDVCSFIHSRLKVTSNLVSIANQVVDTCLHKGSRDNMSIIIIAFPGAPKPTEEAIEAEHRLEKQIEKITRDEIESNQITDYLDLLKCMQNKDDIEGLPPGGGLQSKYHVIERTFKQEFPDRPCESPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN15</accession>
    <name>A0A6I8UN15_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein chibby homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Cby</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358155.4">
        <property type="nucleotide sequence ID" value="XM_001358118.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN15"/>
    <dbReference type="FunCoup" id="A0A6I8UN15">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800974"/>
    <dbReference type="InParanoid" id="A0A6I8UN15"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd07429">
        <property type="entry name" value="Cby_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028118">
        <property type="entry name" value="Chibby_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14645">
        <property type="entry name" value="Chibby"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001358155.4"/>
        </source>
    </evidence>
    <sequence length="135" mass="15816" checksum="F45ADBD77CA7EE29" modified="2020-08-12" version="1">MRVCQLVFDSSTHKQFHSAMPLFNKKFESKPIPLRQNRCNIGIPAVAEDLDDFRQISLNLGNKELRFADGIWMHSSRKGDVDDMLRLKKKFRVLEEENNMSNLKMEFMLDLLAEQASELNELKPREKSTFENVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN47</accession>
    <name>A0A6I8UN47_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Probable cytochrome P450 12e1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Cyp12e1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358189.2">
        <property type="nucleotide sequence ID" value="XM_001358152.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN47"/>
    <dbReference type="FunCoup" id="A0A6I8UN47">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801019"/>
    <dbReference type="InParanoid" id="A0A6I8UN47"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11054">
        <property type="entry name" value="CYP24A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF120">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001358189.2"/>
        </source>
    </evidence>
    <sequence length="527" mass="60078" checksum="119818C75ECAC773" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN89</accession>
    <name>A0A6I8UN89_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4801079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358238.3">
        <property type="nucleotide sequence ID" value="XM_001358201.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN89"/>
    <dbReference type="FunCoup" id="A0A6I8UN89">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801079"/>
    <dbReference type="InParanoid" id="A0A6I8UN89"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019169">
        <property type="entry name" value="Transmembrane_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22168">
        <property type="entry name" value="TMEM26 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22168:SF8">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09772">
        <property type="entry name" value="Tmem26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="475"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358238.3"/>
        </source>
    </evidence>
    <sequence length="535" mass="60226" checksum="2D7565353AB1E5BF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNR7</accession>
    <name>A0A6I8UNR7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase receptor</fullName>
            <ecNumber evidence="13">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">put</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="13"/>
    <dbReference type="RefSeq" id="XP_001358474.3">
        <property type="nucleotide sequence ID" value="XM_001358437.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNR7"/>
    <dbReference type="SMR" id="A0A6I8UNR7"/>
    <dbReference type="FunCoup" id="A0A6I8UNR7">
        <property type="interactions" value="669"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801367"/>
    <dbReference type="InParanoid" id="A0A6I8UNR7"/>
    <dbReference type="OMA" id="WLELCRI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080668">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048179">
        <property type="term" value="C:activin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048185">
        <property type="term" value="F:activin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017002">
        <property type="term" value="F:activin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14053">
        <property type="entry name" value="STKc_ACVR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23615">
        <property type="entry name" value="TFP_LU_ECD_ACVR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000094">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF98">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00653">
        <property type="entry name" value="ACTIVIN2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 13 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026297278" description="Serine/threonine-protein kinase receptor" evidence="14">
        <location>
            <begin position="28"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="205"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001358474.3"/>
        </source>
    </evidence>
    <sequence length="519" mass="58939" checksum="A098912E1260EF8E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNV2</accession>
    <name>A0A6I8UNV2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cleavage stimulation factor subunit 2 tau variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CstF64</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358527.2">
        <property type="nucleotide sequence ID" value="XM_001358490.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNV2"/>
    <dbReference type="FunCoup" id="A0A6I8UNV2">
        <property type="interactions" value="1918"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801432"/>
    <dbReference type="InParanoid" id="A0A6I8UNV2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12671">
        <property type="entry name" value="RRM_CSTF2_CSTF2T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.70:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.630:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000061">
        <property type="entry name" value="cleavage stimulation factor subunit 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="16"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="200"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358527.2"/>
        </source>
    </evidence>
    <sequence length="418" mass="46257" checksum="E155C069125AB2BF" modified="2020-08-12" version="1">MADKLQEQSIMDKSMRSVFVGNIPYEATEEKLKEIFSEVGPVLSLKLVFDRESGKPKGFGFCEYKDQETALSAMRNLNGYEIGGRTLRVDNACTEKSRMEMQQLLQGPQVENPYGEPCEPEDAPELITKTVASLPPEQMYELMKQMKLCIVSNPSEARQMLMLNPQLAYALLQAMVVMRIVDPQQALGMLFKANQMPPVLGGSAHPNAPSGMSMAQQQQQQQIPQQQPTQPGPERGPGPMQPMPVPGPNFNNVHSNDIDLRMGPSGPMPMDPRMMARNLDQDMRQVMPNPVPPPLMDPRTRAQMPAQQQGPAPAPTPYPSDPRQRPMDPRLRAGPVQPPQQVAPQVPPPTQQQQTAAQQLQSRLGAHGVLPSDASDQDKAALIMQVLQLSDEQIAQLPSEQRASILMLKEQIAKSTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP24</accession>
    <name>A0A6I8UP24_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">fray</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001358532.5">
        <property type="nucleotide sequence ID" value="XM_001358495.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP24"/>
    <dbReference type="FunCoup" id="A0A6I8UP24">
        <property type="interactions" value="1398"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801437"/>
    <dbReference type="InParanoid" id="A0A6I8UP24"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000043">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000114">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000068">
        <property type="entry name" value="STE20/SPS1-related proline-alanine-rich protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF16">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="198"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="18"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="124"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="488"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="544"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001358532.5"/>
        </source>
    </evidence>
    <sequence length="718" mass="79350" checksum="D9A8B2780FFCD5AE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP82</accession>
    <name>A0A6I8UP82_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GCS light chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Gamma-ECS regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamylcysteine synthetase regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Glutamate--cysteine ligase modifier subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Gclm</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a catalytic heavy chain and a regulatory light chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358607.2">
        <property type="nucleotide sequence ID" value="XM_001358570.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP82"/>
    <dbReference type="FunCoup" id="A0A6I8UP82">
        <property type="interactions" value="1451"/>
    </dbReference>
    <dbReference type="GeneID" id="4801531"/>
    <dbReference type="KEGG" id="dpo:4801531"/>
    <dbReference type="InParanoid" id="A0A6I8UP82"/>
    <dbReference type="OMA" id="AHEWIPL"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078527">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035226">
        <property type="term" value="F:glutamate-cysteine ligase catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032963">
        <property type="entry name" value="Gclm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13295">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13295:SF4">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="11" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="9">
        <location>
            <begin position="66"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001358607.2"/>
        </source>
    </evidence>
    <sequence length="305" mass="32976" checksum="011F3BEDDB4A83BD" modified="2020-08-12" version="1">MIPCIKKEYQNVVISTGNIINNELGQRKSNEELYDGLKITLHSDPNVERVVVEIDELHGRVQHATEELTNRLTENQRSEISIGAKIFLNNNSTVFIEQAVDTLLNILNVTHVDNVVLAYHPNASGTTSPVANATAKSTTAAPAVKSPCSEANLAPSSVSNWSARNGAQGVIELKELYRSLEQYALNQKITQLGIADLDAEALAELHKSAAVAPTIAQVNLSTCCRVPTELQEFCAAHDIQLNTHGDPELLLPEEQFSGLAPGYTIDWSLRYQVHVRCRGVLTAKGYIVGASQPIPSASTSAKASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPF3</accession>
    <name>A0A6I8UPF3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Glutamate receptor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">KaiR1D</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25">MV-25-SWS-2005</strain>
            <tissue evidence="25">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358619.3">
        <property type="nucleotide sequence ID" value="XM_001358582.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPF3"/>
    <dbReference type="FunCoup" id="A0A6I8UPF3">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801547"/>
    <dbReference type="InParanoid" id="A0A6I8UPF3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UPF3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008328">
        <property type="term" value="C:ionotropic glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004970">
        <property type="term" value="F:glutamate-gated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06382">
        <property type="entry name" value="PBP1_iGluR_Kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000064">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000106">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000060">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000147">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026099513" description="Glutamate receptor 1" evidence="21">
        <location>
            <begin position="29"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="627"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="820"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="22">
        <location>
            <begin position="422"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="23">
        <location>
            <begin position="432"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="677"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="678"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="728"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="18">
        <location>
            <position position="656"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="18">
        <location>
            <position position="683"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="18">
        <location>
            <position position="774"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="740"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_001358619.3"/>
        </source>
    </evidence>
    <sequence length="858" mass="96659" checksum="30C2053263BE7773" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPP8</accession>
    <name>A0A6I8UPP8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Replication stress response regulator SDE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4801759</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SDE2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358800.4">
        <property type="nucleotide sequence ID" value="XM_001358763.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPP8"/>
    <dbReference type="FunCoup" id="A0A6I8UPP8">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801759"/>
    <dbReference type="InParanoid" id="A0A6I8UPP8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053822">
        <property type="entry name" value="SDE2-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF1">
        <property type="entry name" value="SPLICING REGULATOR SDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22782">
        <property type="entry name" value="SDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SDE2-like" evidence="10">
        <location>
            <begin position="90"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="197"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001358800.4"/>
        </source>
    </evidence>
    <sequence length="268" mass="30747" checksum="8CA24073EAC55E7A" modified="2020-08-12" version="1">MQQHKREAQKYNTTKRERRKGREMVINILLNNKELIVFDNEINFNDICTKIEEATHLRRDDYYLVSNGKRLTGDSSHQHESIHCILRQVGGKGGFGSMLRAIGAQIEKTTNREACRDLSGRRLRDINEEKRVRAWLGKQAEREREREERKKRKIEKLLAVPKHDFKDEKYEEARANLADKVSDAFEEGLKQAEEIKEKHAAAASSATEQPSVSGIKRKTKTLADDKTKAKKKKGALWIGDDISGSDTDDSDDSEEEQPETKKKIAIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQC0</accession>
    <name>A0A6I8UQC0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Dihydroorotate dehydrogenase (quinone), mitochondrial</fullName>
            <shortName evidence="16">DHOdehase</shortName>
            <ecNumber evidence="4 16">1.3.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Dhod</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>(S)-dihydroorotate + a quinone = orotate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="16">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Mitochondrion inner membrane</location>
            <topology evidence="1 16">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="4 16"/>
    <dbReference type="RefSeq" id="XP_001359026.5">
        <property type="nucleotide sequence ID" value="XM_001358989.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQC0"/>
    <dbReference type="FunCoup" id="A0A6I8UQC0">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802026"/>
    <dbReference type="InParanoid" id="A0A6I8UQC0"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106430">
        <property type="term" value="F:dihydroorotate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000066">
        <property type="entry name" value="Dihydroorotate dehydrogenase (quinone), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003645">
        <property type="entry name" value="PRK05286.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003652">
        <property type="entry name" value="PRK05286.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF4">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 16" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 16" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="17">
        <location>
            <begin position="77"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001359026.5"/>
        </source>
    </evidence>
    <sequence length="396" mass="43787" checksum="67EF06E8FEF2D7FF" modified="2020-08-12" version="1">MEKSKKFSRMRSLGIVTTGATALLMGITAYNNKDRFFRTFIMPAVRLLPAEASHNLAVMACKYRVYPASQYQDDINLNTQFFGRLLSNPIGIAAGFDKNAEAVDGLKDLGFGFVEVGTVTPTAQEGNPKPRVFRLSEDKAIINRYGFNSDGHEAVLQRLSESRKKENFNAIVGVNLGRNRNTMTPVADYVQGVRLFGPVADYLVINVSSPNTKGLRDMQSKEKLTELLEQVNEARSRLESNRNVPILLKLSPDLEISDMSDIVDVIKRKKSRVDGLIVANTTVSRDNLHDAKWTAEAGGLSGEPLRARSTEMIAQMYQLTNGKVPIIGVGGVSSGYDAYQKFEAGASYVQIYTALVYEGPYLVEQIKDELSKLITQRGHSNIQSVVGTNSKFYLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQI2</accession>
    <name>A0A6I8UQI2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Acetylcholine receptor subunit beta-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">nAChRbeta2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359122.4">
        <property type="nucleotide sequence ID" value="XM_001359085.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQI2"/>
    <dbReference type="FunCoup" id="A0A6I8UQI2">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802144"/>
    <dbReference type="InParanoid" id="A0A6I8UQI2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19031">
        <property type="entry name" value="LGIC_ECD_nAChR_proto_alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000013">
        <property type="entry name" value="Acetylcholine receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000030">
        <property type="entry name" value="Acetylcholine receptor subunit alpha-L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000012">
        <property type="entry name" value="Acetylcholine receptor subunit alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026379336" evidence="18">
        <location>
            <begin position="19"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="31"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="252"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001359122.4"/>
        </source>
    </evidence>
    <sequence length="519" mass="60105" checksum="2FDB079155E5227E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR82</accession>
    <name>A0A6I8UR82_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein Tube</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tub</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359607.4">
        <property type="nucleotide sequence ID" value="XM_001359570.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR82"/>
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        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802744"/>
    <dbReference type="InParanoid" id="A0A6I8UR82"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08308">
        <property type="entry name" value="Death_Tube"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029397">
        <property type="entry name" value="Tube_Death"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14786">
        <property type="entry name" value="Death_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="73"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359607.4"/>
        </source>
    </evidence>
    <sequence length="507" mass="54120" checksum="6DC4363EA72BB443" modified="2020-08-12" version="1">METQQTKNTLKLCCGVEEMRKCFYNAHRKAGQLETQTNSPNKGSETTPFHRKPREEHIVMAKANGWCGSSSKYTRTTELRRVEDNDIYKLATILDVNACWRKLMSIIPKGLDVQACSAAGAVKITANGGPKYTAQDISLIDGVAERLPPDQSISQVMIDEWKTSGKLNERPTVGVLLQLLVEAELFSAADFVALNFLNEPKPERPADGPAAHFSLELGDDMDVDTEATDASYLPNTALNAAAQGSLGFNLDYFDKHMVRRDKSVPQPSASVPPTAPPRSSRHQRNAASPSASTGTTSATATTPNIPNLTILNPSEQLQEPAQVEQPPPQNIPNLSILINSSAASMTTASTALENTSNGSSSTDPPNIPIITLLIDNPAEISGPVAADAVSANSTTTPTAASLNNLPMISALNLNAENGEVFHQDSGSTSSLSNDEDDDDDIDEEGDYADASLPNLSNSEQHNSNNDSSLTTVTGTSGDNSFELTNDSSSASNDDYASNIPNLSELQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR95</accession>
    <name>A0A6I8UR95_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TwdlF</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359625.3">
        <property type="nucleotide sequence ID" value="XM_001359588.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR95"/>
    <dbReference type="FunCoup" id="A0A6I8UR95">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802764"/>
    <dbReference type="InParanoid" id="A0A6I8UR95"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026042580" evidence="1">
        <location>
            <begin position="17"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="2">
        <location>
            <begin position="151"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359625.3"/>
        </source>
    </evidence>
    <sequence length="355" mass="39350" checksum="2DEF47EFFA1F3B29" modified="2020-08-12" version="1">MKQFAVLLCLAALASAAPQGYKYQPQGPALPIGNLRPQTPVSPSGIYSSAAGPALGQITVQPQQPAIQAVQTSAPIPVSQPQVQQTVINSLPQQAVFNPVPQQNLIHAVQQPQPQQQQQQIIYQQPQQLPQVQIQHQPQQIVQTQYVQRPAIVTKDIYIHSAPDETEEIRQDEPQLDSLPIRKNYRIVFIKAPSQNLKYTAAALKRAQSSNEEKTVIYVLSKKPDLTEIQQQLQVTQTESKVHKPEVYFIKYKTQEEAQRAQQEIQAQYDALGGATHISDEGVAPITTVSGGSLNLGHILSQGQAPSIIQQQQQPQIQTIVQQQPIHQQNTFAQQTSSFASNVPQRKYVPAKSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URG9</accession>
    <name>A0A6I8URG9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar complex protein 3 homolog</fullName>
            <shortName evidence="5">NOC3 protein homolog</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Noc3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359751.1">
        <property type="nucleotide sequence ID" value="XM_001359714.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URG9"/>
    <dbReference type="FunCoup" id="A0A6I8URG9">
        <property type="interactions" value="2096"/>
    </dbReference>
    <dbReference type="GeneID" id="4802936"/>
    <dbReference type="KEGG" id="dpo:4802936"/>
    <dbReference type="InParanoid" id="A0A6I8URG9"/>
    <dbReference type="OMA" id="HYCPQVR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071615">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028977">
        <property type="entry name" value="Nucleolar_complex_p3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="9">
        <location>
            <begin position="238"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="8">
        <location>
            <begin position="578"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="454"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR028977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359751.1"/>
        </source>
    </evidence>
    <sequence length="826" mass="94495" checksum="7825A79E8D5813CF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URH7</accession>
    <name>A0A6I8URH7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4802685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359555.2">
        <property type="nucleotide sequence ID" value="XM_001359518.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URH7"/>
    <dbReference type="KEGG" id="dpo:4802685"/>
    <dbReference type="InParanoid" id="A0A6I8URH7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026309728" evidence="2">
        <location>
            <begin position="25"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359555.2"/>
        </source>
    </evidence>
    <sequence length="199" mass="21036" checksum="B897BD20C70B54E4" modified="2020-08-12" version="1">MSVLRLSPTQFLCVLLLAVVCSSAEIDRDEQPDFETEPGAPEPIDGRSFFFLGTLFRRWRNRWMAYHSADPLFAGAAYPINGYYNCPIYGCNPAAIGPQPGYYSRPGGSFNPLNQQQQAQGNSNVYIYQTDQNAANSGSGSGSPAVPLGYGYFGVGSPLRPPPPLPPSSMGGASASAGSYGLGPGQGPVPIPIPVPQFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URM6</accession>
    <name>A0A6I8URM6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Synaptotagmin-14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Syt14</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359615.4">
        <property type="nucleotide sequence ID" value="XM_001359578.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URM6"/>
    <dbReference type="FunCoup" id="A0A6I8URM6">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802754"/>
    <dbReference type="InParanoid" id="A0A6I8URM6"/>
    <dbReference type="OMA" id="NGQQTHT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082014">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08389">
        <property type="entry name" value="C2A_Synaptotagmin-14_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08408">
        <property type="entry name" value="C2B_Synaptotagmin-14_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000231">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000062">
        <property type="entry name" value="synaptotagmin-14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043541">
        <property type="entry name" value="SYT14/14L/16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46129">
        <property type="entry name" value="SYNAPTOTAGMIN 14, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46129:SF2">
        <property type="entry name" value="SYNAPTOTAGMIN 14, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="274"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="429"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359615.4"/>
        </source>
    </evidence>
    <sequence length="570" mass="63807" checksum="65B5671EF20A3FD0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US78</accession>
    <name>A0A6I8US78_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">G patch domain and ankyrin repeat-containing protein 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4803357</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360109.2">
        <property type="nucleotide sequence ID" value="XM_001360072.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US78"/>
    <dbReference type="FunCoup" id="A0A6I8US78">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803357"/>
    <dbReference type="InParanoid" id="A0A6I8US78"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039146">
        <property type="entry name" value="GPANK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20923">
        <property type="entry name" value="BAT4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20923:SF1">
        <property type="entry name" value="G PATCH DOMAIN AND ANKYRIN REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="3">
        <location>
            <begin position="242"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360109.2"/>
        </source>
    </evidence>
    <sequence length="338" mass="38480" checksum="37819FE964523D4F" modified="2020-08-12" version="1">MNSAGDELHPNWLALTTLPLQLKRFVRAGQIECAQQPIKDTKHKINGLDGADAQKFYEEVLHTPATSRAIPTHTHKKSNGSGRDPLLPFDKSKFFRYAISNKVEEMSQMLITDKAMELNACDVYGWTALMMAACEGAKDAVAWLLHNGAHADVRDKSGNTALTLAMLKGHTEVVKILETAPNSEKADIPEEIAGDPTTPFHCRICKRNYKETPWYVHQTSTVHQFNMKAFPPHKLEKFNISSKNRGLQLMVKQGWDREHGLGPNQSGRLYPVKTVLRKQRTGLGIEQSPARVTHFQAFDTNATRRRGPVPQQRRTRQDMQREKVRDWKRNRRLRSELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USA0</accession>
    <name>A0A6I8USA0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pupal cuticle protein Edg-78E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Cpr60D</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360133.4">
        <property type="nucleotide sequence ID" value="XM_001360096.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USA0"/>
    <dbReference type="FunCoup" id="A0A6I8USA0">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803418"/>
    <dbReference type="InParanoid" id="A0A6I8USA0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042302">
        <property type="term" value="F:structural constituent of cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031311">
        <property type="entry name" value="CHIT_BIND_RR_consensus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000618">
        <property type="entry name" value="Insect_cuticle"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00379">
        <property type="entry name" value="Chitin_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00947">
        <property type="entry name" value="CUTICLE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00233">
        <property type="entry name" value="CHIT_BIND_RR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51155">
        <property type="entry name" value="CHIT_BIND_RR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0193">Cuticle</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026045138" evidence="3">
        <location>
            <begin position="21"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360133.4"/>
        </source>
    </evidence>
    <sequence length="95" mass="10432" checksum="D3C7085F5B09B7FF" modified="2020-08-12" version="1">MLEMLKYTLIFALAVVLVSCSEDDRNAELLKSQNLQNLDGAGQFQHEIETSNGISVKAKGNVNGIQGEYFLLGEDGKQIRVTYTADATGFHPQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTP8</accession>
    <name>A0A6I8UTP8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4803811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360469.3">
        <property type="nucleotide sequence ID" value="XM_001360432.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTP8"/>
    <dbReference type="KEGG" id="dpo:4803811"/>
    <dbReference type="InParanoid" id="A0A6I8UTP8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF8">
        <property type="entry name" value="JUVENILE HORMONE-INDUCIBLE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="137"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001360469.3"/>
        </source>
    </evidence>
    <sequence length="439" mass="50872" checksum="65FC5FCCE89E1DA9" modified="2020-08-12" version="1">MSLIETESQWLRTTILPKILESGRLLDTYSASKADTFRVGDIDVDVIGPKEAFMLTLCYRTTIRFEYDGEPFERKMVVKKTPMVPPDVYEAVQFGDLFANEIDFYTKILPQIQKVAEGKFAAPKYYYSELNPLSAIVILSDFAEDSWRVTKDRVGLSLEHVRIAVKYLGKFHGFTYAIKHKNLENFEKLTRNLKESRYANDIVHPQYKVTLQTSVKRAVQAVSTYQPQVDKDFVKLFGFLISDYIQYGRQRVAPREPLATLCHGDYVRNNVAYKYDGKEEPQEIMMFDYQTLRVSSPMIDLSVFLAISVYADVRYTHFDSIFDDYCSALYESYRKHAKDEVPEFMNRTELVKEYIRFLPYSVSITASFLFELVEPMGLSPAEMFNVQVSDEEVIERTMKKGGEVVDKEVAHQLKEMFELSRKYNVQIEDGIDVNALPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU01</accession>
    <name>A0A6I8UU01_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenosine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Adgf-E</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenosine + H2O + H(+) = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. ADGF subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001360610.3">
        <property type="nucleotide sequence ID" value="XM_001360573.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU01"/>
    <dbReference type="FunCoup" id="A0A6I8UU01">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803973"/>
    <dbReference type="InParanoid" id="A0A6I8UU01"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01321">
        <property type="entry name" value="ADGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000017">
        <property type="entry name" value="Adenosine deaminase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013659">
        <property type="entry name" value="A_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006331">
        <property type="entry name" value="ADGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01431">
        <property type="entry name" value="adm_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF39">
        <property type="entry name" value="ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08451">
        <property type="entry name" value="A_deaminase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Adenosine/AMP deaminase N-terminal" evidence="12">
        <location>
            <begin position="34"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Adenosine deaminase" evidence="11">
        <location>
            <begin position="210"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001360610.3"/>
        </source>
    </evidence>
    <sequence length="531" mass="61494" checksum="1C0CCAF4F0B67005" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUB2</accession>
    <name>A0A6I8UUB2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4804443</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361004.1">
        <property type="nucleotide sequence ID" value="XM_001360967.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUB2"/>
    <dbReference type="GeneID" id="4804443"/>
    <dbReference type="KEGG" id="dpo:4804443"/>
    <dbReference type="InParanoid" id="A0A6I8UUB2"/>
    <dbReference type="OMA" id="LNPRMKK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078810">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000257">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF147">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="5"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="164"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361004.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="36589" checksum="64AF4BABEFDBBCF1" modified="2020-08-12" version="1">MLTEFAGNLSHPLEFGHVLEIVAKTIDGAARFHIDLSTAKSTVDPDADIGLHFSCYFRNDTIVRNARVNGVWGEEESAVMDNVTLPNPIVSGEFFMVYILACEDSFHISINSRDFCTFRYRMPLSAIRALEIRDQIQVIRQVDHRTVFPNPWPAIHASDYFKAFSNDQPILFSPGHVIVLTARCSANKRGQFIIKFMDSDTKREELHFSIRFDEQLVVRNSMGKNFEFGAEERHGGFPFKFNEQFKLALAFTEREVLTAVDGFNFFSYAWRTPNAMLNLVGFKVTCIKGLVLQITGVDHLQTGDPTCTAFEKYSRHDYECV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUN5</accession>
    <name>A0A6I8UUN5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pneumococcal serine-rich repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4804516</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361067.4">
        <property type="nucleotide sequence ID" value="XM_001361030.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUN5"/>
    <dbReference type="FunCoup" id="A0A6I8UUN5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804516"/>
    <dbReference type="InParanoid" id="A0A6I8UUN5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="581"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361067.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="792" mass="85904" checksum="489A1AC93E394894" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVA2</accession>
    <name>A0A6I8UVA2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Probable cytochrome P450 6a14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC4804974</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361449.3">
        <property type="nucleotide sequence ID" value="XM_001361412.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVA2"/>
    <dbReference type="FunCoup" id="A0A6I8UVA2">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804974"/>
    <dbReference type="InParanoid" id="A0A6I8UVA2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UVA2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11056">
        <property type="entry name" value="CYP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000042">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050476">
        <property type="entry name" value="Insect_CytP450_Detox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24292:SF100">
        <property type="entry name" value="CYTOCHROME P450 6A16, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026264534" evidence="15">
        <location>
            <begin position="16"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001361449.3"/>
        </source>
    </evidence>
    <sequence length="509" mass="58167" checksum="733966F2B221F2E2" modified="2020-08-12" version="1">MILTFALLGMALALAYNFYRNTYSYWERKGVPNERPLPLIGNVKGIGTKYHFRDINQRIYDKFKGVAPFAGMFTFFKRSALIIDLDLIKQVLIKDFQYFQDRGVYNNVRDEPLTGHLLSLEGDEWRSMRQKLTPVFTSGKIKHMSGVVVEVGHRLAAAMDKAVSAAKVDDGDVEIKDICARFTTDVIGTCAFGLECHSLSDPKAEFRSKGRMIFEKPRHYAIVEFFIFTNAKLAKKLRMKILPDDLSDFFLDAVKNTVDYRMKNGIKRNDFLDELIELRAENQEAAKQGKVIDLSHGLTIEQMAAQAFVFFLAGFETSSSTMAYCLYELAQHPDIQRRVREEIDAVLKNVEGGEITYDALAEMNYLEKVLAETLRKHPIVPQLIREANQNYKVPNTDITIDKGTTVLIPVHNIHHDPEIYPQPERFDPSRFEADQVKSRHPMAYLPFGDGSRNCIGLRFGKIQTKIGLVSLLRRFKFGISNRTEIPLILDTRSFLLATKNGIHLRVKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVZ3</accession>
    <name>A0A6I8UVZ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Deubiquitinase DESI2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4805315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361735.1">
        <property type="nucleotide sequence ID" value="XM_001361698.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVZ3"/>
    <dbReference type="SMR" id="A0A6I8UVZ3"/>
    <dbReference type="FunCoup" id="A0A6I8UVZ3">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="GeneID" id="4805315"/>
    <dbReference type="KEGG" id="dpo:4805315"/>
    <dbReference type="InParanoid" id="A0A6I8UVZ3"/>
    <dbReference type="OMA" id="VYWTKPG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080186">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF80">
        <property type="entry name" value="IP06716P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="37"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001361735.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23327" checksum="6089DE3ED18E0681" modified="2020-08-12" version="1">MFSNGLPCNLSFPSCLSVPKDEIGNEELLPSNMGTREPVILNVYDMYWINEYTTSIGLGVFHSGVEAFGTEFAYGGHPFPFTGVFEISPRDHDELGDQFQFRQSIQIGCTDFTYEEVRRIVEELGNQFRGDRYHLMNNNCNHFSGSLTQILCGQEIPSWVNRLAHFSSCVPFLQRCLPKEWLTPNALQQSITTIQEREDSDNSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY11</accession>
    <name>A0A6I8UY11_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vesicle-associated membrane protein-associated protein B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6902496</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133041.2">
        <property type="nucleotide sequence ID" value="XM_002133005.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY11"/>
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        <property type="interactions" value="45"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0033149">
        <property type="term" value="F:FFAT motif binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF6">
        <property type="entry name" value="AT11025P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="8">
        <location>
            <begin position="8"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002133041.2"/>
        </source>
    </evidence>
    <sequence length="249" mass="28016" checksum="4845110828C9EA9F" modified="2020-08-12" version="1">MAKNIENIMICEPADALTFEGPFNRAIKRCLVIRSLVNKDMAYKLKTTSPRLFYVRPNIGIIGAKSSVSIEIFMPPTLPLSPDPFGKRHKFLIVVGEAPEPVVDLQNFFKDLDPANAWEGRVRCELVLPMPENNNGFGDIILDESIRQTVGGEPNEELERTYDALEVSTLVDMQILQEKVSNLEKVRLELSQQIQDLRDSAEKGQHAKKVLLRQRSTMFLFSAACVAVGAILFFGNFGKDFLKLIEKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZ09</accession>
    <name>A0A6I8UZ09_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Odorant receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Or13a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133203.2">
        <property type="nucleotide sequence ID" value="XM_002133167.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZ09"/>
    <dbReference type="FunCoup" id="A0A6I8UZ09">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902688"/>
    <dbReference type="InParanoid" id="A0A6I8UZ09"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF44">
        <property type="entry name" value="ODORANT RECEPTOR 13A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU351113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002133203.2"/>
        </source>
    </evidence>
    <sequence length="417" mass="48722" checksum="2A3E40D7C4D4CD34" modified="2020-08-12" version="1">MFNLQQYTAITFRLPVQCVWLKLNGSWPFVPSSRKDNSSMCSILYTAWAWYVIVSVGITIGFQTAYLVTHLSDIIMTTENCCTTFMGALNFVRLLHLRFNQRKFRQLIEQFERDIWIPENTHISVNAECHKRMFTFTIMTGLLSCLICMYCALPLVEIFFRTGVDAVEKPFPYKMLFPYDPYSSWMRYVFTYMFTSYAGICVVTTLFAEDSIFGFFITYTCGQFRLLHERVDNVLTAAKERADEQHLQLQRLHNIVHQHNKIIRFAKCLEDFFNPILLVNLMISSLLICMVGFQIITGKNMFIGEYVKFIVYISSAISQLYVLCENGDALIIHSTNTARHLYGCDWENPDIRNFYSYTSMSHQLRNDLKFMILCSQRPVRITAFKFSTLSLQSFTAILSTSMSYFTLLRSLYYEDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UZL4</accession>
    <name>A0A6I8UZL4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alanine--glyoxylate aminotransferase 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6901500</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134186.2">
        <property type="nucleotide sequence ID" value="XM_002134150.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UZL4"/>
    <dbReference type="FunCoup" id="A0A6I8UZL4">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6901500"/>
    <dbReference type="InParanoid" id="A0A6I8UZL4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UZL4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000058">
        <property type="entry name" value="ethanolamine-phosphate phospho-lyase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688:SF13">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002134186.2"/>
        </source>
    </evidence>
    <sequence length="497" mass="54488" checksum="5ABA0D424D434923" modified="2020-08-12" version="1">MPFAQEQSKSSTVASEQLSKTETIKLRNKHIGQACQLFYRSDPLKIVRGQGQYMFDEEGTRYLDCINNVAHVGHCHPEVVRAGALQMATISTNNRFLHDELVQCAQTLTSKMPEPLSVCFFVNSGSEANDLALRLARNFTKRQDVITLDHAYHGHLQAVMEVSPYKFNQPGGEKKPDYVHVAPCPDTYGGQFTDKMYPNADLGALYAQPIEEICQRQLAKGQGVAAFIAESLQSCGGQILPPPGYFRAVYDAVRSSGGLCIADEVQVGFGRVGTHYWAFETQGVVPDIVCVAKPMGNGHPVGAVVTTPEIAQAFHATGVAYFNTYGGNPVSCAIANAVMRVIDQEGLQQNALQLGDYLLRECSHLKQEFECIGDVRGVGLFVGIELVKDREGRIPDTKSARWVVNRMKQLHQVLVSSDGPNDNVIKLKPPMCFSRENADEFLLAFRECLATLTQDKMATEAATAAATTTHGVIATAAETLANKTKLFERQDRLIKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V088</accession>
    <name>A0A6I8V088_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134649.3">
        <property type="nucleotide sequence ID" value="XM_002134613.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V088"/>
    <dbReference type="KEGG" id="dpo:6900948"/>
    <dbReference type="InParanoid" id="A0A6I8V088"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V088">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026312179" description="Kazal-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134649.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12258" checksum="12384EEA5F9BD17F" modified="2020-08-12" version="1">MSTTLVMGLLFLACTFLYISACLSTSVRSPCPPEESCTQAEGQSTAICGLDEDENCIRKYPSKCLMDIAACHAGRTFTDYSSVYCAMESFLCEQSSTYERWTVFFGYEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V107</accession>
    <name>A0A6I8V107_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein draper isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">drpr</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135253.3">
        <property type="nucleotide sequence ID" value="XM_002135217.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V107"/>
    <dbReference type="FunCoup" id="A0A6I8V107">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900896"/>
    <dbReference type="InParanoid" id="A0A6I8V107"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V107">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Multiple epidermal growth factor-like domains 10"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042635">
        <property type="entry name" value="MEGF10/SREC1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043:SF8">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026347196" evidence="8">
        <location>
            <begin position="21"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="804"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="domain" description="EMI" evidence="10">
        <location>
            <begin position="27"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="187"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="225"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="400"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="491"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="662"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1005"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1023"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="687"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002135253.3"/>
        </source>
    </evidence>
    <sequence length="1043" mass="114540" checksum="390A9B9981F4BE4C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1G4</accession>
    <name>A0A6I8V1G4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TBC1 domain family member whacked isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">wkd</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002136239.3">
        <property type="nucleotide sequence ID" value="XM_002136203.3"/>
    </dbReference>
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        <property type="interactions" value="669"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899287"/>
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    <dbReference type="OMA" id="AIQPQYM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243664">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000008">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
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        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF4">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="113"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="369"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002136239.3"/>
        </source>
    </evidence>
    <sequence length="402" mass="46324" checksum="1E93334EC683DCD6" modified="2020-08-12" version="1">MPSLFFSHYRHSIDCLELIPKDDKFAVVAAGELALAMATAPRSLDTISLCSTVSSCPDRNGFYGGFQRTDKPKEPLSKAQIIAREKKWLYMIENWSIYMSKNYKKIRDRCRKGIPKSVRPKAWFYLSGAYLLKKKNPNVYEELLEKPGNPATIEEIKKDKHRQFPFHEMFLDEQKVGQIELFNVLKAYSIYNPKVGFCQAQAPIAAFLLMHLPAEDAFWVFVSVCDVYLQDYFIPGLEVIQNDAGILEGLLKKTCPPVYRHLQKHKVEPLLYMTDWFLCGMTRTLPWETLLRVWDCFLAEGIRVIFKVALVIIGASLSRHKVRKTCTGLCETLAVMRSPPEHILEEEFIINNMMRLNLRVEDFQIEHTRQKARRAKQKAQQDGNPNDSGPSNSNGRRNMPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V212</accession>
    <name>A0A6I8V212_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC6896700</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002136914.2">
        <property type="nucleotide sequence ID" value="XM_002136878.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V212"/>
    <dbReference type="KEGG" id="dpo:6896700"/>
    <dbReference type="InParanoid" id="A0A6I8V212"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.190:FF:000001">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 21 kDa subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="entry name" value="atu1810 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219:SF8">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002136914.2"/>
        </source>
    </evidence>
    <sequence length="182" mass="20907" checksum="1644DA6D7356707D" modified="2020-08-12" version="1">MSLLRRVMCRTTATSLQLFTSRCASSKDTDAPLDLNTALARPEDLEKHSKLSGTITVPTAVDLSPISGVPSQQLKERRVRIHIPPKNAMQSGTNYLNKWLIEFDNRERWENPLMGWASSGDPLSNLNLQFGSKNEAITYCERNGWRWYVDRDSDRGQKPKKQRFKSYGVNFSWNKRTRLSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V249</accession>
    <name>A0A6I8V249_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kunitz-type serine protease inhibitor PPTI-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6896879</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137069.1">
        <property type="nucleotide sequence ID" value="XM_002137033.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V249"/>
    <dbReference type="GeneID" id="6896879"/>
    <dbReference type="KEGG" id="dpo:6896879"/>
    <dbReference type="InParanoid" id="A0A6I8V249"/>
    <dbReference type="OMA" id="ICMEPEA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248380">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF217">
        <property type="entry name" value="BOOPHILIN-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0800">Toxin</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026079221" evidence="7">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="8">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002137069.1"/>
        </source>
    </evidence>
    <sequence length="93" mass="10960" checksum="8E226CA622C248A6" modified="2020-08-12" version="1">MVKLMLNFAILSLLLCLASATKKELLEHRKKICMQPSDYGSCKERRLRWYYDPTANACKPFTYSGCGGTYNRFERETECWNYCMDPDIFYETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V307</accession>
    <name>A0A6I8V307_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6897372</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137525.3">
        <property type="nucleotide sequence ID" value="XM_002137489.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V307"/>
    <dbReference type="SMR" id="A0A6I8V307"/>
    <dbReference type="FunCoup" id="A0A6I8V307">
        <property type="interactions" value="1695"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897372"/>
    <dbReference type="InParanoid" id="A0A6I8V307"/>
    <dbReference type="OMA" id="ACENITR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248638">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434:SF6">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002137525.3"/>
        </source>
    </evidence>
    <sequence length="143" mass="16663" checksum="9857749269AC1A18" modified="2020-08-12" version="1">MASGSRITILPQSKEALLKSYNVRLKEDVRSMLENFEETLKLARRDSHSQISRTTQSEQDALEMQIRASNMVRAGESLMKLVADLKQYLILNDFHSVNEAITNNSQLFRSTQIECDKKLMKLRDEMAMDLYDLEEEYYTSIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V315</accession>
    <name>A0A6I8V315_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chaperone protein DnaJ-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6896757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002136964.2">
        <property type="nucleotide sequence ID" value="XM_002136928.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V315"/>
    <dbReference type="KEGG" id="dpo:6896757"/>
    <dbReference type="InParanoid" id="A0A6I8V315"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF22">
        <property type="entry name" value="MOLECULAR CHAPERONE HSP40_DNAJ FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="76"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002136964.2"/>
        </source>
    </evidence>
    <sequence length="285" mass="32748" checksum="BCC2BAE090584C07" modified="2020-08-12" version="1">MFRLVLVNARALLGGQEKGRKSCPMVGVDRLYARYQFKRPKPFYYPDRKEEAERKAEAATKEIVEMENSGLRPKNFCYKVLGVSKYATIEEIRDAYLVLAKRYYPDVTRSKRALNQFQEISNSYRILTDANKRLEYDHSKNVAIEKALSKPPKKLRQKWHKTKQLQSAISANEEEATLGCNKLFDLWYLRKCEKCGGTPQLLSDRDVEKEPCRRCHGTGKVFSQSCGTHADILSDVEMLVIKSKGVRRNGVGKRIDTVKVFVPHKLSAEQRLLILALAETEDPIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3L5</accession>
    <name>A0A6I8V3L5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6897759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137864.4">
        <property type="nucleotide sequence ID" value="XM_002137828.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3L5"/>
    <dbReference type="KEGG" id="dpo:6897759"/>
    <dbReference type="InParanoid" id="A0A6I8V3L5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002137864.4"/>
        </source>
    </evidence>
    <sequence length="195" mass="22906" checksum="83572E42B2672906" modified="2020-08-12" version="1">MSFQSYYSPKAGTMRFQLIICAVIGLFLAFRITINDAVIFKFTNAVCESYNQSWFVFHNCRLKAVSRSKVFFNMNGTILHPANDIKIHMRIFKKANGYKPWLFDVTCDACLHLRKRNTPFLSIVYGLFKPYTNINHTCPYVGPQIITDFYLRPELLRLPFPTGDYMLSMRWIFDKKLQFDTNISFTYVEDLIKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3V8</accession>
    <name>A0A6I8V3V8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome c oxidase assembly factor 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6898168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in an early step of the mitochondrial complex IV assembly process.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PET191 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138246.1">
        <property type="nucleotide sequence ID" value="XM_002138210.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3V8"/>
    <dbReference type="FunCoup" id="A0A6I8V3V8">
        <property type="interactions" value="401"/>
    </dbReference>
    <dbReference type="GeneID" id="6898168"/>
    <dbReference type="KEGG" id="dpo:6898168"/>
    <dbReference type="InParanoid" id="A0A6I8V3V8"/>
    <dbReference type="OMA" id="KKTPKEC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245895">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018793">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_Pet191"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28627">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28627:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10203">
        <property type="entry name" value="Pet191_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_002138246.1"/>
        </source>
    </evidence>
    <sequence length="80" mass="9411" checksum="818B08BFA6784F89" modified="2020-08-12" version="1">MMRYEGNEKLADETACAGVRADLKMCLLESDCCRVDRKTPRQCLMDNNVPPECQVLRNTFYECKRSLLDNRQRFRGRKGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V460</accession>
    <name>A0A6I8V460_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="4">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mei-W68</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_002138430.1">
        <property type="nucleotide sequence ID" value="XM_002138394.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V460"/>
    <dbReference type="FunCoup" id="A0A6I8V460">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="GeneID" id="6898377"/>
    <dbReference type="KEGG" id="dpo:6898377"/>
    <dbReference type="InParanoid" id="A0A6I8V460"/>
    <dbReference type="OMA" id="IETAGMF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245793">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="11">
        <location>
            <begin position="54"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="12">
        <location>
            <begin position="164"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002138430.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="36947" checksum="963565E3B49D5F84" modified="2020-08-12" version="1">MSGDVEGIVVRLLASLVLHGDATFMVPRMGPVSRDSGNGNGYRRVSYNNRRSRHRFCLVVYMLAKIHRLQVNGGSYTVRGLYYSDPQLIRTQRHIAAARLDVCHILNTSPTRLGVLSASKGLMAGDIRMLMTNGDVLDCNVYSGAIILPTDTEKVERIVTQADFVLVVEKESVFESLLARDLFGTFGRRCILVTGKGYPDCSTRRILHRLSTECHLPAYILVDADPFGIEIMLVYRLGSQSMSFTSGDLATPTLRWIGLHPSEIPALSSGAVALSSGDNKKIDDILARDYIGPGVRQELRSLQQIQQKAEIESVIDFLSTDYIPNKINRNLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4D5</accession>
    <name>A0A6I8V4D5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Facilitated trehalose transporter Tret1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6898520</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Trehalose transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138561.2">
        <property type="nucleotide sequence ID" value="XM_002138525.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4D5"/>
    <dbReference type="FunCoup" id="A0A6I8V4D5">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898520"/>
    <dbReference type="InParanoid" id="A0A6I8V4D5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000055">
        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF1">
        <property type="entry name" value="GH07001P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="276"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="337"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="9"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002138561.2"/>
        </source>
    </evidence>
    <sequence length="445" mass="47944" checksum="8C9D0DA77FBC391D" modified="2020-08-12" version="1">MSGWKQYLAGVAAAFGAFCLGASIGWSGPVEKEVRGGGAYKFTPSSAEWGWISSLLTLGAATSCIPAGVLIGIFGRKITMLGLAPPFFIGWLLIIFAQKAFMLMIGRFIVGFCGGAFCITAPMYNTEIAELSKRGIMGCFFQLLIVHGVLYGFIVGAYAKVKMMNILCGILPIIFFVLFIWMPESPVYLAQKGKNDKAEKSLKFLRGKDADVSAESNQMASEGNKEKVKPMQALCRKNTLKSLGISMMLMVFQQVTGINAIIFYSTGIFTDAGTGFSPAISTIIIGVVMVIATIVSIMLIDRVGRKILLLVSAALMFVTTLIMAVYFQWLLKKNVGWLPVLAVCVFISGFSFGFGPVPWLLMAELFAEDAKPVAGAIAGTTNWMCAFIVTLAFPLIKDGFGAAACFWIFAAVSFAAIIFVLFLVPETKGKTLNEIQGMIAGGKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4K3</accession>
    <name>A0A6I8V4K3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138744.2">
        <property type="nucleotide sequence ID" value="XM_002138708.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4K3"/>
    <dbReference type="KEGG" id="dpo:6898766"/>
    <dbReference type="InParanoid" id="A0A6I8V4K3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="209"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138744.2"/>
        </source>
    </evidence>
    <sequence length="295" mass="35080" checksum="770BAC85051E2996" modified="2020-08-12" version="1">MGSMDTKKYSEKCSIPDLDDLLPNRKYHCKMCPRQQVFKGLPLYNRHMKKIHKIEFEEKDCFHCPVKHCRRHEHMPNNVIMTKFHMVRRHYQDSHLVKKYKCQQCKRRFLLKEQYLKHKCRLGLKEETDSETENDENEFVDVENIDDTNSYVSRAVKTPLKESMDSKNVGGNNVEIKDAMDNYLLCWSGEKHCTKCRRKYKRRHSCQEHICNLCSRIFYRKSDLAAHEFTLAHISKQKDKVLMEDMVLIEDMQNLIRRIESKDFQDESLINTLGALVPTFQKIQDSIPKTSARTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4M7</accession>
    <name>A0A6I8V4M7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zwei Ig domain protein zig-8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dpr1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138526.3">
        <property type="nucleotide sequence ID" value="XM_002138490.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4M7"/>
    <dbReference type="FunCoup" id="A0A6I8V4M7">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898484"/>
    <dbReference type="InParanoid" id="A0A6I8V4M7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V4M7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000129">
        <property type="entry name" value="CLUMA_CG018772, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001633">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF7">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="56"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="168"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138526.3"/>
        </source>
    </evidence>
    <sequence length="380" mass="41418" checksum="BD02251F69B4C996" modified="2020-08-12" version="1">MSARLTSLSPSSASASASSLLPARANFGLLNWLISWLLLSMALLRGYSAAIAPTPPTTTTTTISPSNLLPYFDFDVPRNLTVTVGQTGFLHCRVERLGDKDVSWIRKRDLHILTAGGTTYTSDQRFQVLRPDGSANWTLQIKYPQPRDSGVYECQINTEPKMSLSYTFNVVELKAEIFGPRDLMVKTGSDINLTCKIMQGPHELGNIFWYKGSEMLDGKGENEIDSSMARIRVEDDWSDGLTSSRLKIKHAMPGDTGNYTCVPTVAKTSSVYVHVIIGEHPAAMQHNSSSNSNSFYCGICCMLLSIVSCCLQHWYESGSGYVSTTAMPSTGSVFNFPRHATLQTRGTGVTRTAVSTTAAVAAAPTSNMLRARPAAAQCMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6C0</accession>
    <name>A0A6I8V6C0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6899113</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the XRCC4-XLF family. XLF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002139030.3">
        <property type="nucleotide sequence ID" value="XM_002138994.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6C0"/>
    <dbReference type="KEGG" id="dpo:6899113"/>
    <dbReference type="InParanoid" id="A0A6I8V6C0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.210.10">
        <property type="entry name" value="DNA double-strand break repair and VJ recombination XRCC4, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.450">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052287">
        <property type="entry name" value="NHEJ_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038051">
        <property type="entry name" value="XRCC4-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235:SF1">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="216"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002139030.3"/>
        </source>
    </evidence>
    <sequence length="442" mass="49086" checksum="A9CBA09762494F2A" modified="2020-08-12" version="1">MLTVIEINGRNFIMNFLASGPDTGIYLQCHDLALEQSYTESVVPSDFTKRLKMLNSRVKFPEDDAREVLVSGGDLPTYASLSEADQDAGCVLQLKYKMLNNMPLKWEWHLKRMDRALFYRKAFIDSISCGSRMRDQVFALAKQLQAKDEELKQYRIEGAQLRRGTVATKLFDMEAFRATNKQMLSGSAIYQQLTDAYEDSSLKIVPGSVEAPKVSGLTSSQAPPSSNVTPKLSPRNRKRKALESAKFHIERKVLRRRAQGPIEYKESQSSQEEIAGAAEHIKQEPPVVKGQHIKKESVDSAAARPVAAEHIKQEPPVVREQNIKKESVDPSSARTLAAVERSREERHAVLARAVCAAEIFNPKSSVEKASKIVAPAVTELAVAVSKKTINQEPLYDGAAIASETINIDTEPPPANEAPTGLDSVLVSLKEMESFLFKTKNQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6K9</accession>
    <name>A0A6I8V6K9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC15382900</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_004444214.3">
        <property type="nucleotide sequence ID" value="XM_004444157.3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:15382900"/>
    <dbReference type="InParanoid" id="A0A6I8V6K9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15293">
        <property type="entry name" value="7tmC_GPR158-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12913">
        <property type="entry name" value="PDC1_MCP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043458">
        <property type="entry name" value="GPR158/179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054714">
        <property type="entry name" value="GPR158_179_extracellular"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32546">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 158-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32546:SF29">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22572">
        <property type="entry name" value="GPR158_179_EC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026232307" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="19">
        <location>
            <begin position="44"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="891"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="924"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="956"/>
            <end position="975"/>
        </location>
    </feature>
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    </protein>
    <gene>
        <name evidence="8 9 10" type="primary">rin</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="9 10">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015037530.2">
        <property type="nucleotide sequence ID" value="XM_015182044.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037531.2">
        <property type="nucleotide sequence ID" value="XM_015182045.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037532.2">
        <property type="nucleotide sequence ID" value="XM_015182046.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VB37"/>
    <dbReference type="KEGG" id="dpo:6897419"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12229">
        <property type="entry name" value="RRM_G3BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001406">
        <property type="entry name" value="AT27578p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000010">
        <property type="entry name" value="Ras GTPase-activating protein-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039539">
        <property type="entry name" value="Ras_GTPase_bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693:SF20">
        <property type="entry name" value="AT27578P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="NTF2" evidence="6">
        <location>
            <begin position="16"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="494"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="418"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="579"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="440"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="594"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="619"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="633"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="677"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015037530.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015037531.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015037532.2"/>
        </source>
    </evidence>
    <sequence length="696" mass="74987" checksum="2692F6C1B01EC6FB" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBN4</accession>
    <name>A0A6I8VBN4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein hdly isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hdly</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037306.2">
        <property type="nucleotide sequence ID" value="XM_015181820.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBN4"/>
    <dbReference type="FunCoup" id="A0A6I8VBN4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VBN4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VBN4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026060946" evidence="2">
        <location>
            <begin position="42"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015037306.2"/>
        </source>
    </evidence>
    <sequence length="493" mass="55211" checksum="662B75D13269AA5D" modified="2020-08-12" version="1">MKRHNKLKEMNRSLPSNGSRSLGLPALLFTLVLCLVAQAASRPPTEDESDIAVLPPDMDNVEIHQVRMVDGVMQADHPVIMYKEDFVSEDYDPSKNETATTVHHKKHKHSRTHHHRQEPHQEKEEEKIVFDVLPDKPIVLNDDLEAAPKKNLEEAAELVKPTKEEWLRKSHKKYHSRQRRQTYTPCSHSSYYQTPSHAVYFQPPAPPSPYYYLPAKPSQRLPVSGKKPILPGKLPIWQDKTDENPTNPMSIGSRDDFLLHNSDPAEADFSIFNQPRPNPNEISGSSGSSSASGSDRGRDNISNSPPAVNNQVWGPISNSRPVSRPSWSQTQTTSRPWDSPPVTAAPSWSRPTPRATPSPKVTNCVWAIVNCCSKGSKKIRYNCFEEFGCSGAFWGINPCADETVLDADERLGETYPLDSAVPVAESAPLAPQPSYRQDALRFPEEYDYQPGSKCQRASQLCCSLYSRNIGSLYECFLYQGCEQTLSNIVANCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VC80</accession>
    <name>A0A6I8VC80_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear protein MDM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13 14" type="primary">LOC6897882</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Microtubule-binding protein that negatively regulates centriole duplication. Binds to and stabilizes microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MDM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015038215.2">
        <property type="nucleotide sequence ID" value="XM_015182729.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038217.2">
        <property type="nucleotide sequence ID" value="XM_015182731.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232394.1">
        <property type="nucleotide sequence ID" value="XM_033376503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VC80"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046600">
        <property type="term" value="P:negative regulation of centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029136">
        <property type="entry name" value="MDM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32078">
        <property type="entry name" value="NUCLEAR PROTEIN MDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32078:SF1">
        <property type="entry name" value="NUCLEAR PROTEIN MDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15501">
        <property type="entry name" value="MDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="196"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="394"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    <evidence type="ECO:0000256" key="10">
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        <source>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_015038217.2"/>
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        <source>
            <dbReference type="RefSeq" id="XP_033232394.1"/>
        </source>
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    <sequence length="976" mass="106376" checksum="2E261518DDCBC80B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDT9</accession>
    <name>A0A6I8VDT9_DROPS</name>
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        <submittedName>
            <fullName evidence="17">Metabotropic glutamate receptor 2 isoform X1</fullName>
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        <name evidence="17" type="primary">mtt</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
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    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
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    <comment type="function">
        <text evidence="11">G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors.</text>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
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        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
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            <begin position="1212"/>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015039202.2"/>
        </source>
    </evidence>
    <sequence length="1444" mass="155564" checksum="C609CA92DAFD5999" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VG39</accession>
    <name>A0A6I8VG39_DROPS</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="primary">LOC4804604</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
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        </source>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
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            <dbReference type="RefSeq" id="XP_015040042.2"/>
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    <sequence length="979" mass="105855" checksum="42792EEED7B531B5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGY3</accession>
    <name>A0A6I8VGY3_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_015185144.2"/>
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        <property type="component" value="Chromosome 3"/>
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        <location>
            <begin position="12"/>
            <end position="36"/>
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        <location>
            <begin position="57"/>
            <end position="82"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015040630.2"/>
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    </evidence>
    <sequence length="145" mass="16083" checksum="D7D38F6A0747504D" modified="2020-08-12" version="1">MRLAKCLCCIPLRVGVILTGCLFGATDLIIGSYGLYMVIRREFPDNVVEFFRTMDTLTCVACFTGTFYLMAFNDLMLIYGAIRKKSGYIGPWLMVNFVVLICTMVTALVSGIAIIRIGEAEPRPHAPHRFIHSLFQSSSVIACSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VH42</accession>
    <name>A0A6I8VH42_DROPS</name>
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        <recommendedName>
            <fullName evidence="16">Phosphatidylinositol 4-kinase alpha</fullName>
            <ecNumber evidence="4">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Pi4KIIIalpha</name>
    </gene>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and HYCC (HYCC1 or HYCC2). Interacts with TMEM150A; regulating recruitment to the plasma membrane. Interacts with TTC7A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_015185750.2"/>
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        <property type="interactions" value="2266"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00871">
        <property type="entry name" value="PI4Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="18">
        <location>
            <begin position="1590"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="17">
        <location>
            <begin position="1870"/>
            <end position="2135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_015041236.2"/>
        </source>
    </evidence>
    <sequence length="2151" mass="242268" checksum="55283197923823A6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VH88</accession>
    <name>A0A6I8VH88_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26531966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041328.2">
        <property type="nucleotide sequence ID" value="XM_015185842.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VH88"/>
    <dbReference type="KEGG" id="dpo:26531966"/>
    <dbReference type="InParanoid" id="A0A6I8VH88"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VH88">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015041328.2"/>
        </source>
    </evidence>
    <sequence length="154" mass="17287" checksum="19C6E005CF7AB46D" modified="2020-08-12" version="1">MNPTPVGIGFGFGRKPPPNNAKMRSRWRPRSRSRSRPSALSVQGGVESDIPHTAAAEPTETFQATVQKHSEDSNHSRIQDQHQHQDQHHQQQHHHHHSKQHSQLTTLAVLLLALVAINHLDSLLPWAPATATRIEGARQRHGSPGNYNNDKNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VLJ4</accession>
    <name>A0A6I8VLJ4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iron-sulfur clusters transporter ABCB7, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-binding cassette sub-family B member 7, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ABCB7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(glutathione)4[2Fe(III)-2S] cluster(in) + ATP + H2O = (glutathione)4[2Fe(III)-2S] cluster(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67028"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167627"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67029"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015043830.2">
        <property type="nucleotide sequence ID" value="XM_015188344.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VLJ4"/>
    <dbReference type="FunCoup" id="A0A6I8VLJ4">
        <property type="interactions" value="1457"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VLJ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VLJ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="161"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="486"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="76"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="722"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015043830.2"/>
        </source>
    </evidence>
    <sequence length="744" mass="81186" checksum="2537ED8E9C730D5B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VM28</accession>
    <name>A0A6I8VM28_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sperm flagellar protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC26533208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015044128.2">
        <property type="nucleotide sequence ID" value="XM_015188642.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VM28"/>
    <dbReference type="KEGG" id="dpo:26533208"/>
    <dbReference type="InParanoid" id="A0A6I8VM28"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010441">
        <property type="entry name" value="CH_2"/>
    </dbReference>
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        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052111">
        <property type="entry name" value="Spermatogenesis_Ciliary_MAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12509:SF9">
        <property type="entry name" value="SPERM FLAGELLAR PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12509">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06294">
        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CH-like" evidence="2">
        <location>
            <begin position="16"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015044128.2"/>
        </source>
    </evidence>
    <sequence length="147" mass="17105" checksum="6B84B27F085ED24B" modified="2020-08-12" version="1">MSHIRELNSCESRDLHLWLQEQRIDLNAKKRVKFSDVLPVATIAQTVYPRLVDLIHYTPSRHSVANKLQNWKVFNFTVLKKLNITLSELYMKELAQGQEGAIELLFYELFVRPLKIASEEDRDYTTGNPDQIKTSFGSEYGSKTTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMA9</accession>
    <name>A0A6I8VMA9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Protein sprouty</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">sty</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sprouty family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352860.3">
        <property type="nucleotide sequence ID" value="XM_001352824.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043923.2">
        <property type="nucleotide sequence ID" value="XM_015188437.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VMA9"/>
    <dbReference type="KEGG" id="dpo:4811880"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VMA9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040037">
        <property type="term" value="P:negative regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007875">
        <property type="entry name" value="Sprouty"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051192">
        <property type="entry name" value="Sprouty_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12365:SF7">
        <property type="entry name" value="PROTEIN SPROUTY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12365">
        <property type="entry name" value="SPROUTY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05210">
        <property type="entry name" value="Sprouty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51227">
        <property type="entry name" value="SPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="111"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352860.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015043923.2"/>
        </source>
    </evidence>
    <sequence length="633" mass="66183" checksum="0D6B978A233B30E8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VND4</accession>
    <name>A0A6I8VND4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Exo84</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the EXO84 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232571.1">
        <property type="nucleotide sequence ID" value="XM_033376680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VND4"/>
    <dbReference type="FunCoup" id="A0A6I8VND4">
        <property type="interactions" value="1674"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VND4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VND4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01226">
        <property type="entry name" value="PH_RalBD_exo84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1220:FF:000003">
        <property type="entry name" value="Exocyst 84, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000376">
        <property type="entry name" value="Exocyst complex component 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1220">
        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033961">
        <property type="entry name" value="Exo84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032403">
        <property type="entry name" value="Exo84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042560">
        <property type="entry name" value="Exo84_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16528">
        <property type="entry name" value="Exo84_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="172"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="271"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="292"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033232571.1"/>
        </source>
    </evidence>
    <sequence length="709" mass="80822" checksum="0AEDFBD43058DB25" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VP73</accession>
    <name>A0A6I8VP73_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4801802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232564.1">
        <property type="nucleotide sequence ID" value="XM_033376673.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VP73">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VP73"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VP73">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1285"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1405"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1436"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1483"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1614"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="467"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1285"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1452"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1535"/>
            <end position="1556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232564.1"/>
        </source>
    </evidence>
    <sequence length="1668" mass="184177" checksum="AFDAA4AB0CCC891E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQH2</accession>
    <name>A0A6I8VQH2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC13036386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232979.1">
        <property type="nucleotide sequence ID" value="XM_033377088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQH2"/>
    <dbReference type="KEGG" id="dpo:13036386"/>
    <dbReference type="InParanoid" id="A0A6I8VQH2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033232979.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="29112" checksum="BDD32B1EEAC78CD1" modified="2020-08-12" version="1">MALFKQYHDSKAVLYNFTFEGCKVLDRSTRNPVSNFFMEMLSTYSNLNHSCPYNKDTEFDYCYTKSASRVAKFFFETSNMNQSCPYNTYSSIEFTNVNCTSLDSKFGEFEYCYLKSVNRTYKYFSAKFKVYELPISSMKVNFSLWKRYNGYKPFLYNVTFDACKFFANNNSNRVIKFFYESFTAYSNINHTCPLNHDIIMEKLPIDFVNHRMTEILPFPEGQYLVEVRWIRSGTYLAFGKLYATLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQV1</accession>
    <name>A0A6I8VQV1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233431.1">
        <property type="nucleotide sequence ID" value="XM_033377540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQV1"/>
    <dbReference type="KEGG" id="dpo:6898335"/>
    <dbReference type="InParanoid" id="A0A6I8VQV1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VQV1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233431.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="37619" checksum="F48287FE7CB2DFC4" modified="2020-08-12" version="1">MLLLYEHKCLVRLPTVASNDIKVEPQSLQRCRNDQRRPLAQLWPTTPCLSHQKTNFIGKQLKKEEKEQTLDGIWTPGSSGFWRLVRWRRVLETRRGCMYLNRLPAWMAAVVLCGRGVEKPRSLVLETRRGCMYLNRVASDSISSATRFPCIRTTCTGATGAPGTLIKTGQSRSLAKDVACIPPSYDGLPELAAAGVRQDYPFLGELAEASKRLLFWTDISSHQAIIRARVDGNEHVDLEEVTTLALDQQSDIPCVSIKIRASMHISQVSAFGRFVSDDKTGLERSAITPVCTVAQSVSTSAWPAGYDTQYSRRMLVPDSDALMLLEGKENYGVYPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VRM8</accession>
    <name>A0A6I8VRM8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="8">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Sod3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="8">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="8"/>
    <dbReference type="RefSeq" id="XP_033233548.1">
        <property type="nucleotide sequence ID" value="XM_033377657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VRM8"/>
    <dbReference type="FunCoup" id="A0A6I8VRM8">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VRM8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 8" id="KW-0186">Copper</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026282678" description="Superoxide dismutase [Cu-Zn]" evidence="9">
        <location>
            <begin position="19"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="10">
        <location>
            <begin position="43"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033233548.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="26062" checksum="C2C328950EB8C564" modified="2020-08-12" version="1">MMQSIAITLALCATICAAAQTRNTPIEAIAYVSGPAQADGSQVKGNVTFTQNDCGQNVHVRVQLEGLKEGKHGFHVHEKGDLSNGCASTGAHYNPDKVDHGGPDHEVRHVGDLGNLEVNSSGVIDITYTDKVISLTGNRGIIGRAVVVHELEDDLGLGDHVDSKKTGNAGGRIGCGVIGVNGAPQQLQEATAPIRTPSARHVHHQPPLYYVPHEEPQDHVVYPLQRYPSYPYPYPYPHPFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXV7</accession>
    <name>A0A6I8VXV7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LOW QUALITY PROTEIN: ras-interacting protein RIP3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235399.1">
        <property type="nucleotide sequence ID" value="XM_033379508.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8VXV7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816575"/>
    <dbReference type="InParanoid" id="A0A6I8VXV7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="423"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="484"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="554"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="743"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="748"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235399.1"/>
        </source>
    </evidence>
    <sequence length="770" mass="84218" checksum="1C17940652832626" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W106</accession>
    <name>A0A6I8W106_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Art8</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="RefSeq" id="XP_033237031.1">
        <property type="nucleotide sequence ID" value="XM_033381140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W106"/>
    <dbReference type="FunCoup" id="A0A6I8W106">
        <property type="interactions" value="507"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184280"/>
    <dbReference type="InParanoid" id="A0A6I8W106"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000024">
        <property type="entry name" value="Arginine methyltransferase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF122">
        <property type="entry name" value="ARGININE METHYLTRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="7">
        <location>
            <begin position="151"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033237031.1"/>
        </source>
    </evidence>
    <sequence length="342" mass="38387" checksum="B462254BE501927D" modified="2020-08-12" version="1">MSNPYFDEYENLEVHELMLKDRPRQIAYCNAILGNKDIFKDKVVLDVGAGTGILSAFCAKAGARLVYAVEASNVATKVALDLMQDNGLTSIVKVIHSRVEELVLPVSSEKVDIIVSEWMGFYLLHEGMLGSVLLARDKFLKADGLMFPTECTIFVAPCSVPSLFDYWQNIDGIKMDSFAKKLRTQKSTRPEITQLDPKNLLHEGVVLHWMNLLDVDMAELEEVRFKDVVAAQRGGNHQGFCIWFEVLFPGNEAVFLSTSPFAPETHWKQCVVVLPQDACETVDEKSPIAFQISMTRSASDMRKYNLEVELLDPNIEEHPVPCSCHMTKCILTEAHLKTINTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W1R5</accession>
    <name>A0A6I8W1R5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ascorbate ferrireductase (transmembrane)</fullName>
            <ecNumber evidence="11">7.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4817914</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.2.1.3" evidence="11"/>
    <dbReference type="RefSeq" id="XP_033236659.1">
        <property type="nucleotide sequence ID" value="XM_033380768.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A6I8W1R5"/>
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        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817914"/>
    <dbReference type="InParanoid" id="A0A6I8W1R5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W1R5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140571">
        <property type="term" value="F:transmembrane ascorbate ferrireductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140575">
        <property type="term" value="F:transmembrane monodehydroascorbate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08554">
        <property type="entry name" value="Cyt_b561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045150">
        <property type="entry name" value="CYB561D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422:SF43">
        <property type="entry name" value="ASCORBATE FERRIREDUCTASE (TRANSMEMBRANE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15422">
        <property type="entry name" value="OS05G0565100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="13">
        <location>
            <begin position="17"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033236659.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="25635" checksum="67F4ED6A737425FB" modified="2020-08-12" version="1">MAEGSTGATAWLQVQSLLNSINHMLIFLVATFFFVLARSLDFKDTAMHMFMTGIGFHVLIAQAMMSHYKVNPITRWLSHRNKSRFHGILQIIGGAMVLLGGLGKFSNKEVHFNTWHGRVGSAATFFCAASIVGGLANYFQPKLALKLFPPSELRFRHNLFGLVTYSLGMGAIYLGYYSKFFTKHVDNDFIPGMMLVTSLVYVLTIISPVSSLLSKLKYRRQKKQEKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W403</accession>
    <name>A0A6I8W403_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Protein Exd1 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">LOC6901088</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3">MV-25-SWS-2005</strain>
            <tissue evidence="2 3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238074.1">
        <property type="nucleotide sequence ID" value="XM_033382183.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238075.1">
        <property type="nucleotide sequence ID" value="XM_033382184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W403"/>
    <dbReference type="KEGG" id="dpo:6901088"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W403">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990923">
        <property type="term" value="C:PET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052144">
        <property type="entry name" value="piRNA_biogenesis_EXD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46628">
        <property type="entry name" value="PIRNA BIOGENESIS PROTEIN EXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46628:SF1">
        <property type="entry name" value="PIRNA BIOGENESIS PROTEIN EXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033238074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238075.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="32152" checksum="98E2B508060D3357" modified="2020-08-12" version="1">MADSDDDSVASFKTAEEISPSREKTIFPLMSHELSSLERKLNRIVYIQQADAAYHKALNDMRDQLSISVIAEPSLYGRHRPVAVLVVATANQTYVFDIQALGAFFPELKKLLEDKYPRKVVHYSHRISDQLFYKHQITLTGFSDTFVALCVARQDKSACSLPEAISSVLNIPLRELLSDEVTGVSESRQLFTARPLSGDQFRFLGRMAILQHLMHDRLTFGHICAGMQRMSATFSQSFCRFRNGCDVAPYMGPKSRFGFQYIDTYYKSAVGSLPVDYEIDHAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W470</accession>
    <name>A0A6I8W470_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC117184409</name>
        <name evidence="17" type="synonym">me31B</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17 18">MV-25-SWS-2005</strain>
            <tissue evidence="17 18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_033236157.1">
        <property type="nucleotide sequence ID" value="XM_033380266.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237489.1">
        <property type="nucleotide sequence ID" value="XM_033381598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W470"/>
    <dbReference type="SMR" id="A0A6I8W470"/>
    <dbReference type="KEGG" id="dpo:117184099"/>
    <dbReference type="KEGG" id="dpo:117184409"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000114">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000364">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="89"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="269"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033236157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033237489.1"/>
        </source>
    </evidence>
    <sequence length="459" mass="52031" checksum="BDA7AEDF0151865B" modified="2020-08-12" version="1">MMTEKISSGHTNLSNKGIINDQKILANISDDMGWKSKLKLPPKDNRFKTTDVTDTRGNEFEEFCLKRELLMGIFEKGWERPSPIQEAAIPIALSGKDVLARAKNGTGKTGAYCIPVLEQIDPTKDYIQALVMVPTRELALQTSQICIELAKHLDIRVMVTTGGTILKDDILRIYQKVQLIIATPGRILDLMDKKVADMSHCRILVLDEADKLLSLDFQGMLDHVILKLPKDPQILLFSATFPLTVKNFMEKHLREPYEINLMEELTLKGVTQYYAFVQERQKVHCLNTLFSKLQINQSIIFCNSTQRVELLAKKITELGYCCYYIHAKMAQAHRNRVFHDFRQGLCRNLVCSDLFTRGIDVQAVNVVINFDFPRMAETYLHRIGRSGRFGHLGIAINLITYEDRFDLHRIEKELGTEIKPIPKVIDPALYVANVSGSSGEICNNSDLNTSANEEGNVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4M4</accession>
    <name>A0A6I8W4M4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein jim lovell isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4815361</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033237649.1">
        <property type="nucleotide sequence ID" value="XM_033381758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W4M4"/>
    <dbReference type="FunCoup" id="A0A6I8W4M4">
        <property type="interactions" value="498"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8W4M4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W4M4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF102">
        <property type="entry name" value="PIPSQUEAK, ISOFORM O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50960">
        <property type="entry name" value="HTH_PSQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="46"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="HTH psq-type" evidence="6">
        <location>
            <begin position="576"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="3">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="282"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="366"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="290"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="389"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033237649.1"/>
        </source>
    </evidence>
    <sequence length="702" mass="76771" checksum="B7E2B62C3F742057" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6D0</accession>
    <name>A0A6I8W6D0_DROPS</name>
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        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117184612</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238915.1">
        <property type="nucleotide sequence ID" value="XM_033383024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6D0"/>
    <dbReference type="KEGG" id="dpo:117184612"/>
    <dbReference type="InParanoid" id="A0A6I8W6D0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033238915.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="40832" checksum="72C64191DD16CAD1" modified="2020-08-12" version="1">MAEETMANIDKEKIVEWLLEKEIIFPARATLRQLRKLAISGGMDEVSESLVNKSDIESEEKVSENEQIDDMKEEELLDAAIRVAEKKKKLAALMVMDNHVTDDIRMVKQLIAPFSASENDEASEWVLNFERICGGVNESSNFKLRCVRMLMKAGTEADLFMRVDKSKTYDEFKGNFLKTFGRSNSTADVVLLLKETFFNPEKNSVMGYILRMEEIAMRADIEEKLTVQFIIDGFRDRSSSIALLYSASTIGKLKELARQYEALRKSTQTNFRRTGMTASGNDRSQARCYNCSAHGHYASSCPAPKREKGSCFQCGSMQHQVKDCQQKPMSAQRSVGTASNPPMDDREENNIFVPIVNQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7J6</accession>
    <name>A0A6I8W7J6_DROPS</name>
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        <recommendedName>
            <fullName evidence="1">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239257.1">
        <property type="nucleotide sequence ID" value="XM_033383366.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8W7J6">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184699"/>
    <dbReference type="InParanoid" id="A0A6I8W7J6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="1">
        <location>
            <begin position="128"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239257.1"/>
        </source>
    </evidence>
    <sequence length="249" mass="28252" checksum="5044285B8C3CCDED" modified="2020-08-12" version="1">MVGSRHLRRLVKKERDEIARKLELVQRQGVERENQRELDSAELDIQENNEVVQEDIGIVQDGLAEHRLMAAISSLEKKVDASFSVLEKKVDDSISGLEKKVGVLSETLVEVVALLKLQIKKEPDVDNFVKFPIQSTMELEHLNSIITPELTDFYINKIKSLLGTSSSMASSLKHVLDEKLILDYNVDGTSGKSSLKNVKEFYYVLESAVKIKIPDEPADKIIRKAIHNIKNSHFQKTSRAKKFKLDNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7Y2</accession>
    <name>A0A6I8W7Y2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein Ir92a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Ir92a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033239485.1">
        <property type="nucleotide sequence ID" value="XM_033383594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W7Y2"/>
    <dbReference type="KEGG" id="dpo:4801855"/>
    <dbReference type="InParanoid" id="A0A6I8W7Y2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050907">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000244">
        <property type="entry name" value="Ionotropic receptor 41a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF40">
        <property type="entry name" value="IONOTROPIC RECEPTOR 41A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="671"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="11">
        <location>
            <begin position="405"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="154"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033239485.1"/>
        </source>
    </evidence>
    <sequence length="699" mass="78533" checksum="3E49296A95F1F707" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W823</accession>
    <name>A0A6I8W823_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238774.1">
        <property type="nucleotide sequence ID" value="XM_033382883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W823"/>
    <dbReference type="InParanoid" id="A0A6I8W823"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238774.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="27509" checksum="E1A1AC285575666B" modified="2020-08-12" version="1">MDSIGDDSLFQARTRVPVRFVYCFSSRVSQKRNQKCHSKDSPWSAFVSYLYGYTGITFAFAAVSADLLLYKFRCRVAADISLLPENQPTTTTSNQQHVQILRSVPLRRRGLCGGQARGDCPTGVQRLLLPPGGRLPLLVCLHRRLHSPRLRRLLRLLRVSLCVRLLRIPLCRLLPLIRDWPLEKHNLDTGPPHQPPNNMDTPICTCNHLQPPATTAPPPPPAHLCTAPPSALSFFFLSLPKHTAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W855</accession>
    <name>A0A6I8W855_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC117184746</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239462.1">
        <property type="nucleotide sequence ID" value="XM_033383571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W855"/>
    <dbReference type="KEGG" id="dpo:117184746"/>
    <dbReference type="InParanoid" id="A0A6I8W855"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="407"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="146"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="448"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033239462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="52913" checksum="12BC5AA943A5C21C" modified="2020-08-12" version="1">MEGEPVNPSVCFLCGEEFGSLQLYKTRCGHEFHKACIVPYSKENAKCPRCGRVTFELQGGASVSGTQTAKQQAGSKEKASGTPATGSANNNPGSASVDGGNIGIMITQAVRAMQNDLLTQLSEQMAQIIQTNVAAHLQLTGRDQHTRNIAPDHSSQGQEAGGGRESAGFYREGRDTPRSLASDLSQRPDKVVHIMNGWKLRFSGDAEGISADNFIYRVEALTHATMESNFAVLCSNASILFDGKAREFFWRFHKSVVVVRWDVLCQALKKQFRDTRTDVDIREAIRDRKQKEKEGFDAFYDAIVQLMDSLESPLSEKSVVDILRRNLRPEVRHELLNIKITSVGELREICRRRESFLEDVRRNYGYQKSVPFRRQVAELVEEHMSDDATEFSEGEGEAEIGALALVCWNCHKEGHRYQDCESKCKIFCYGCGIPNIYKPACEKCQKKNGRASTQPRQLQSVRRQKSANPDASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDY9</accession>
    <name>A0A6I8WDY9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Aminopeptidase N isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">LOC13036301</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <strain evidence="15">MV2-25</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241603.1">
        <property type="nucleotide sequence ID" value="XM_033385712.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241604.1">
        <property type="nucleotide sequence ID" value="XM_033385713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDY9"/>
    <dbReference type="KEGG" id="dpo:13036301"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000013">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000005">
        <property type="entry name" value="Aminopeptidase N-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF18">
        <property type="entry name" value="FI02158P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 17" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634640" evidence="11">
        <location>
            <begin position="23"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="48"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="296"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="575"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033241603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033241604.1"/>
        </source>
    </evidence>
    <sequence length="929" mass="108233" checksum="99FA33A9D6977A5A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEF1</accession>
    <name>A0A6I8WEF1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4802838</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241768.1">
        <property type="nucleotide sequence ID" value="XM_033385877.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8WEF1">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802838"/>
    <dbReference type="InParanoid" id="A0A6I8WEF1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16474">
        <property type="entry name" value="RING-H2_RNF111-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000479">
        <property type="entry name" value="E3 ubiquitin-protein ligase Arkadia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45676">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45676:SF41">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="1276"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="131"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="277"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="361"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="449"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="803"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="873"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1027"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1146"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="456"/>
            <end position="472"/>
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        <location>
            <begin position="480"/>
            <end position="496"/>
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        <location>
            <begin position="508"/>
            <end position="518"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="543"/>
            <end position="560"/>
        </location>
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        <location>
            <begin position="576"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="608"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="825"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="878"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1033"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1147"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1160"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1183"/>
            <end position="1194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033241768.1"/>
        </source>
    </evidence>
    <sequence length="1343" mass="147019" checksum="924173803A8E5501" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8H5J3</accession>
    <name>A0ABD8H5J3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit K</fullName>
            <shortName evidence="5">eIF3k</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">eIF-3 p25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">eIF3k</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit K family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360371.1">
        <property type="nucleotide sequence ID" value="XM_001360334.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8H5J3"/>
    <dbReference type="SMR" id="A0ABD8H5J3"/>
    <dbReference type="GeneID" id="4803694"/>
    <dbReference type="KEGG" id="dpo:4803694"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000212">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.250:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit K"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03010">
        <property type="entry name" value="eIF3k"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009374">
        <property type="entry name" value="eIF3k"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016020">
        <property type="entry name" value="Transl_init_fac_sub12_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13022">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13022:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="46"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00057041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001360371.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="25801" checksum="AC19AF9C00A6CD67" modified="2025-06-18" version="1">MSHLVKMENGQSQTIQEMLGCIERYNPDHLKTLEAYIQDQAKNNTYDLEANLAVLKLYQFNPHMLNFEITYTILLKSLTNLPHTDFVMAKCLLLPQQMKDENIQTIIDLADILERADFTLFWQRAEVNRTMFRHISGFHDSIRKFVSHVVSITFQTIKKDLLKELLGGIEDSTLESWIKRNGWKHQGQDLIVVAMQDDKIKTKNITEKIEFENVGALMAQCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>B5DJU8</accession>
    <accession>A0A6I8V9R4</accession>
    <name>B5DJU8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Ankyrin repeat domain-containing protein 29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">LOC6902653</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133169.2">
        <property type="nucleotide sequence ID" value="XM_002133133.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036747.1">
        <property type="nucleotide sequence ID" value="XM_015181261.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235410.1">
        <property type="nucleotide sequence ID" value="XM_033379519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DJU8"/>
    <dbReference type="SMR" id="B5DJU8"/>
    <dbReference type="STRING" id="46245.B5DJU8"/>
    <dbReference type="GeneID" id="6902653"/>
    <dbReference type="KEGG" id="dpo:6902653"/>
    <dbReference type="eggNOG" id="KOG4177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_464830_0_0_1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250388">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DJU8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031436">
        <property type="term" value="C:BRCA1-BARD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000540">
        <property type="entry name" value="GD22794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="42"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="141"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133169.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015036747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033235410.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="34524" checksum="6AE1970592A1A267" modified="2016-01-20" version="2">MSLKKETPSDVQLHMAAMRGDEVALLRLLDSGKVHVDCKDEDGTTPLILAAAGGHTYCVMELLDQGADPNTRRLTGTTPLFFAAQGGHLDVVKILMKAGAKVDTASADGGTPLFVAAQGGHVKIVRELLDCGANVNAHMKDRATPVFIASQNGHRTVLSLLILAHADIDIKRIDGATPLWIAAQMGQDHICKVLLQNGANVDTVRCDGATPLFKAAHKGHAAVTTVLLKYRPNLGLLPNGETALHAAAMFGHMTVCKQLVAAGSDVLLKNQEDLTALELAHQQKYTSICDYLQERVRAIVARNSKTMVPATSSVTSTSTVTSMPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>B5DRT7</accession>
    <name>SOSSC_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>SOSS complex subunit C homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA28629</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SOSS-C family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379070">
        <property type="protein sequence ID" value="EDY74198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DRT7"/>
    <dbReference type="SMR" id="B5DRT7"/>
    <dbReference type="FunCoup" id="B5DRT7">
        <property type="interactions" value="433"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DRT7"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0288609">
        <property type="protein sequence ID" value="FBpp0287047"/>
        <property type="gene ID" value="FBgn0249989"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900242"/>
    <dbReference type="eggNOG" id="KOG3420">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145773_0_0_1"/>
    <dbReference type="InParanoid" id="B5DRT7"/>
    <dbReference type="OMA" id="VMETQHM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249989">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031821">
        <property type="entry name" value="SOSSC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31526">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31526:SF2">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15925">
        <property type="entry name" value="SOSSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000385325" description="SOSS complex subunit C homolog">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="125" mass="13243" checksum="449693B907342FDC" modified="2008-10-14" version="1">MAFPTTSAQQAETNRKILEDIQTKKQLLAGGIINLGLSNTNQMPSPQLLGQPTVAPEFLPQGVGLPTNATPPRSAFNPTSSTTLGFFIPQDSYFGNSFIPVLPRLEPLPTTTAPATTTASHIAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-09-05" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I5AMN4</accession>
    <accession>A0A6I8V5D0</accession>
    <name>I5AMN4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Small nuclear ribonucleoprotein E</fullName>
            <shortName evidence="12">snRNP-E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Sm protein E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SmE</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736146.1">
        <property type="nucleotide sequence ID" value="XM_003736098.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I5AMN4"/>
    <dbReference type="SMR" id="I5AMN4"/>
    <dbReference type="FunCoup" id="I5AMN4">
        <property type="interactions" value="2033"/>
    </dbReference>
    <dbReference type="STRING" id="46245.I5AMN4"/>
    <dbReference type="GeneID" id="4817452"/>
    <dbReference type="KEGG" id="dpo:4817452"/>
    <dbReference type="eggNOG" id="KOG1774">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125186_1_0_1"/>
    <dbReference type="InParanoid" id="I5AMN4"/>
    <dbReference type="OMA" id="VPPINCI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075029">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="I5AMN4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01718">
        <property type="entry name" value="Sm_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000013">
        <property type="entry name" value="Small nuclear ribonucleoprotein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12 15" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="17"/>
            <end position="92"/>
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    <evidence type="ECO:0000256" key="1">
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022884"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365053"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_003736146.1"/>
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    </evidence>
    <sequence length="94" mass="11057" checksum="64F7845E27A20482" modified="2012-09-05" version="1">MSFKSNPKVQKVMVQPINLIFRYLQNRSRVQVWLYENISLRIEGHIVGFDEYMNLVLDDAEEVYVKTRQRKSLGRIMLKGDNITLIQNVSPSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29CI8</accession>
    <accession>A0A6I8UA84</accession>
    <name>Q29CI8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">S-phase kinase-associated protein 1-like isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein, isoform A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Dpse\GA14255</name>
        <name evidence="9" type="synonym">LOC4811669</name>
        <name evidence="7" type="ORF">Dpse_GA14255</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="450" first="203" last="218">
            <title>Evolution of genes and genomes on the Drosophila phylogeny.</title>
            <authorList>
                <consortium name="Drosophila 12 Genomes Consortium"/>
                <person name="Clark A.G."/>
                <person name="Eisen M.B."/>
                <person name="Smith D.R."/>
                <person name="Bergman C.M."/>
                <person name="Oliver B."/>
                <person name="Markow T.A."/>
                <person name="Kaufman T.C."/>
                <person name="Kellis M."/>
                <person name="Gelbart W."/>
                <person name="Iyer V.N."/>
                <person name="Pollard D.A."/>
                <person name="Sackton T.B."/>
                <person name="Larracuente A.M."/>
                <person name="Singh N.D."/>
                <person name="Abad J.P."/>
                <person name="Abt D.N."/>
                <person name="Adryan B."/>
                <person name="Aguade M."/>
                <person name="Akashi H."/>
                <person name="Anderson W.W."/>
                <person name="Aquadro C.F."/>
                <person name="Ardell D.H."/>
                <person name="Arguello R."/>
                <person name="Artieri C.G."/>
                <person name="Barbash D.A."/>
                <person name="Barker D."/>
                <person name="Barsanti P."/>
                <person name="Batterham P."/>
                <person name="Batzoglou S."/>
                <person name="Begun D."/>
                <person name="Bhutkar A."/>
                <person name="Blanco E."/>
                <person name="Bosak S.A."/>
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                <person name="Brand A.D."/>
                <person name="Brent M.R."/>
                <person name="Brooks A.N."/>
                <person name="Brown R.H."/>
                <person name="Butlin R.K."/>
                <person name="Caggese C."/>
                <person name="Calvi B.R."/>
                <person name="Bernardo de Carvalho A."/>
                <person name="Caspi A."/>
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                <person name="Chapple C."/>
                <person name="Chatterji S."/>
                <person name="Chinwalla A."/>
                <person name="Civetta A."/>
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                <person name="Hultmark D."/>
                <person name="Huntley M.A."/>
                <person name="Jaffe D.B."/>
                <person name="Jagadeeshan S."/>
                <person name="Jeck W.R."/>
                <person name="Johnson J."/>
                <person name="Jones C.D."/>
                <person name="Jordan W.C."/>
                <person name="Karpen G.H."/>
                <person name="Kataoka E."/>
                <person name="Keightley P.D."/>
                <person name="Kheradpour P."/>
                <person name="Kirkness E.F."/>
                <person name="Koerich L.B."/>
                <person name="Kristiansen K."/>
                <person name="Kudrna D."/>
                <person name="Kulathinal R.J."/>
                <person name="Kumar S."/>
                <person name="Kwok R."/>
                <person name="Lander E."/>
                <person name="Langley C.H."/>
                <person name="Lapoint R."/>
                <person name="Lazzaro B.P."/>
                <person name="Lee S.J."/>
                <person name="Levesque L."/>
                <person name="Li R."/>
                <person name="Lin C.F."/>
                <person name="Lin M.F."/>
                <person name="Lindblad-Toh K."/>
                <person name="Llopart A."/>
                <person name="Long M."/>
                <person name="Low L."/>
                <person name="Lozovsky E."/>
                <person name="Lu J."/>
                <person name="Luo M."/>
                <person name="Machado C.A."/>
                <person name="Makalowski W."/>
                <person name="Marzo M."/>
                <person name="Matsuda M."/>
                <person name="Matzkin L."/>
                <person name="McAllister B."/>
                <person name="McBride C.S."/>
                <person name="McKernan B."/>
                <person name="McKernan K."/>
                <person name="Mendez-Lago M."/>
                <person name="Minx P."/>
                <person name="Mollenhauer M.U."/>
                <person name="Montooth K."/>
                <person name="Mount S.M."/>
                <person name="Mu X."/>
                <person name="Myers E."/>
                <person name="Negre B."/>
                <person name="Newfeld S."/>
                <person name="Nielsen R."/>
                <person name="Noor M.A."/>
                <person name="O'Grady P."/>
                <person name="Pachter L."/>
                <person name="Papaceit M."/>
                <person name="Parisi M.J."/>
                <person name="Parisi M."/>
                <person name="Parts L."/>
                <person name="Pedersen J.S."/>
                <person name="Pesole G."/>
                <person name="Phillippy A.M."/>
                <person name="Ponting C.P."/>
                <person name="Pop M."/>
                <person name="Porcelli D."/>
                <person name="Powell J.R."/>
                <person name="Prohaska S."/>
                <person name="Pruitt K."/>
                <person name="Puig M."/>
                <person name="Quesneville H."/>
                <person name="Ram K.R."/>
                <person name="Rand D."/>
                <person name="Rasmussen M.D."/>
                <person name="Reed L.K."/>
                <person name="Reenan R."/>
                <person name="Reily A."/>
                <person name="Remington K.A."/>
                <person name="Rieger T.T."/>
                <person name="Ritchie M.G."/>
                <person name="Robin C."/>
                <person name="Rogers Y.H."/>
                <person name="Rohde C."/>
                <person name="Rozas J."/>
                <person name="Rubenfield M.J."/>
                <person name="Ruiz A."/>
                <person name="Russo S."/>
                <person name="Salzberg S.L."/>
                <person name="Sanchez-Gracia A."/>
                <person name="Saranga D.J."/>
                <person name="Sato H."/>
                <person name="Schaeffer S.W."/>
                <person name="Schatz M.C."/>
                <person name="Schlenke T."/>
                <person name="Schwartz R."/>
                <person name="Segarra C."/>
                <person name="Singh R.S."/>
                <person name="Sirot L."/>
                <person name="Sirota M."/>
                <person name="Sisneros N.B."/>
                <person name="Smith C.D."/>
                <person name="Smith T.F."/>
                <person name="Spieth J."/>
                <person name="Stage D.E."/>
                <person name="Stark A."/>
                <person name="Stephan W."/>
                <person name="Strausberg R.L."/>
                <person name="Strempel S."/>
                <person name="Sturgill D."/>
                <person name="Sutton G."/>
                <person name="Sutton G.G."/>
                <person name="Tao W."/>
                <person name="Teichmann S."/>
                <person name="Tobari Y.N."/>
                <person name="Tomimura Y."/>
                <person name="Tsolas J.M."/>
                <person name="Valente V.L."/>
                <person name="Venter E."/>
                <person name="Venter J.C."/>
                <person name="Vicario S."/>
                <person name="Vieira F.G."/>
                <person name="Vilella A.J."/>
                <person name="Villasante A."/>
                <person name="Walenz B."/>
                <person name="Wang J."/>
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                <person name="Wilson D."/>
                <person name="Wilson R.K."/>
                <person name="Wing R.A."/>
                <person name="Wolfner M.F."/>
                <person name="Wong A."/>
                <person name="Wong G.K."/>
                <person name="Wu C.I."/>
                <person name="Wu G."/>
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                <person name="Yang H.P."/>
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                <person name="Yorke J.A."/>
                <person name="Yoshida K."/>
                <person name="Zdobnov E."/>
                <person name="Zhang P."/>
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                <person name="Zimin A.V."/>
                <person name="Baldwin J."/>
                <person name="Abdouelleil A."/>
                <person name="Abdulkadir J."/>
                <person name="Abebe A."/>
                <person name="Abera B."/>
                <person name="Abreu J."/>
                <person name="Acer S.C."/>
                <person name="Aftuck L."/>
                <person name="Alexander A."/>
                <person name="An P."/>
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                <person name="Arachi H."/>
                <person name="Azer M."/>
                <person name="Bachantsang P."/>
                <person name="Barry A."/>
                <person name="Bayul T."/>
                <person name="Berlin A."/>
                <person name="Bessette D."/>
                <person name="Bloom T."/>
                <person name="Blye J."/>
                <person name="Boguslavskiy L."/>
                <person name="Bonnet C."/>
                <person name="Boukhgalter B."/>
                <person name="Bourzgui I."/>
                <person name="Brown A."/>
                <person name="Cahill P."/>
                <person name="Channer S."/>
                <person name="Cheshatsang Y."/>
                <person name="Chuda L."/>
                <person name="Citroen M."/>
                <person name="Collymore A."/>
                <person name="Cooke P."/>
                <person name="Costello M."/>
                <person name="D'Aco K."/>
                <person name="Daza R."/>
                <person name="De Haan G."/>
                <person name="DeGray S."/>
                <person name="DeMaso C."/>
                <person name="Dhargay N."/>
                <person name="Dooley K."/>
                <person name="Dooley E."/>
                <person name="Doricent M."/>
                <person name="Dorje P."/>
                <person name="Dorjee K."/>
                <person name="Dupes A."/>
                <person name="Elong R."/>
                <person name="Falk J."/>
                <person name="Farina A."/>
                <person name="Faro S."/>
                <person name="Ferguson D."/>
                <person name="Fisher S."/>
                <person name="Foley C.D."/>
                <person name="Franke A."/>
                <person name="Friedrich D."/>
                <person name="Gadbois L."/>
                <person name="Gearin G."/>
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                <person name="Giannoukos G."/>
                <person name="Goode T."/>
                <person name="Graham J."/>
                <person name="Grandbois E."/>
                <person name="Grewal S."/>
                <person name="Gyaltsen K."/>
                <person name="Hafez N."/>
                <person name="Hagos B."/>
                <person name="Hall J."/>
                <person name="Henson C."/>
                <person name="Hollinger A."/>
                <person name="Honan T."/>
                <person name="Huard M.D."/>
                <person name="Hughes L."/>
                <person name="Hurhula B."/>
                <person name="Husby M.E."/>
                <person name="Kamat A."/>
                <person name="Kanga B."/>
                <person name="Kashin S."/>
                <person name="Khazanovich D."/>
                <person name="Kisner P."/>
                <person name="Lance K."/>
                <person name="Lara M."/>
                <person name="Lee W."/>
                <person name="Lennon N."/>
                <person name="Letendre F."/>
                <person name="LeVine R."/>
                <person name="Lipovsky A."/>
                <person name="Liu X."/>
                <person name="Liu J."/>
                <person name="Liu S."/>
                <person name="Lokyitsang T."/>
                <person name="Lokyitsang Y."/>
                <person name="Lubonja R."/>
                <person name="Lui A."/>
                <person name="MacDonald P."/>
                <person name="Magnisalis V."/>
                <person name="Maru K."/>
                <person name="Matthews C."/>
                <person name="McCusker W."/>
                <person name="McDonough S."/>
                <person name="Mehta T."/>
                <person name="Meldrim J."/>
                <person name="Meneus L."/>
                <person name="Mihai O."/>
                <person name="Mihalev A."/>
                <person name="Mihova T."/>
                <person name="Mittelman R."/>
                <person name="Mlenga V."/>
                <person name="Montmayeur A."/>
                <person name="Mulrain L."/>
                <person name="Navidi A."/>
                <person name="Naylor J."/>
                <person name="Negash T."/>
                <person name="Nguyen T."/>
                <person name="Nguyen N."/>
                <person name="Nicol R."/>
                <person name="Norbu C."/>
                <person name="Norbu N."/>
                <person name="Novod N."/>
                <person name="O'Neill B."/>
                <person name="Osman S."/>
                <person name="Markiewicz E."/>
                <person name="Oyono O.L."/>
                <person name="Patti C."/>
                <person name="Phunkhang P."/>
                <person name="Pierre F."/>
                <person name="Priest M."/>
                <person name="Raghuraman S."/>
                <person name="Rege F."/>
                <person name="Reyes R."/>
                <person name="Rise C."/>
                <person name="Rogov P."/>
                <person name="Ross K."/>
                <person name="Ryan E."/>
                <person name="Settipalli S."/>
                <person name="Shea T."/>
                <person name="Sherpa N."/>
                <person name="Shi L."/>
                <person name="Shih D."/>
                <person name="Sparrow T."/>
                <person name="Spaulding J."/>
                <person name="Stalker J."/>
                <person name="Stange-Thomann N."/>
                <person name="Stavropoulos S."/>
                <person name="Stone C."/>
                <person name="Strader C."/>
                <person name="Tesfaye S."/>
                <person name="Thomson T."/>
                <person name="Thoulutsang Y."/>
                <person name="Thoulutsang D."/>
                <person name="Topham K."/>
                <person name="Topping I."/>
                <person name="Tsamla T."/>
                <person name="Vassiliev H."/>
                <person name="Vo A."/>
                <person name="Wangchuk T."/>
                <person name="Wangdi T."/>
                <person name="Weiand M."/>
                <person name="Wilkinson J."/>
                <person name="Wilson A."/>
                <person name="Yadav S."/>
                <person name="Young G."/>
                <person name="Yu Q."/>
                <person name="Zembek L."/>
                <person name="Zhong D."/>
                <person name="Zimmer A."/>
                <person name="Zwirko Z."/>
                <person name="Jaffe D.B."/>
                <person name="Alvarez P."/>
                <person name="Brockman W."/>
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                <person name="Grabherr M."/>
                <person name="Kleber M."/>
                <person name="Mauceli E."/>
                <person name="MacCallum I."/>
            </authorList>
            <dbReference type="PubMed" id="17994087"/>
            <dbReference type="DOI" id="10.1038/nature06341"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47768" last="E47768">
            <title>Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.</title>
            <authorList>
                <person name="English A.C."/>
                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CH475467">
        <property type="protein sequence ID" value="EAL29385.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001352258.2">
        <property type="nucleotide sequence ID" value="XM_001352222.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29CI8"/>
    <dbReference type="SMR" id="Q29CI8"/>
    <dbReference type="STRING" id="46245.Q29CI8"/>
    <dbReference type="GeneID" id="4811669"/>
    <dbReference type="KEGG" id="dpo:4811669"/>
    <dbReference type="HOGENOM" id="CLU_059252_4_0_1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074284">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000018">
        <property type="entry name" value="S-phase kinase-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044737612" evidence="4">
        <location>
            <begin position="18"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component POZ" evidence="6">
        <location>
            <begin position="30"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="5">
        <location>
            <begin position="139"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EAL29385.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001352258.2"/>
        </source>
    </evidence>
    <sequence length="189" mass="21602" checksum="3964C61EF9117143" modified="2016-01-20" version="2">MCGFLLICISSTRTCICVDSCVEKISKMPNIKLQSSDEEIFDTDIQIAKCSGTIKTMLEDCGMEDDENAIVPLPNVNSTILRKVLTWAHYHKDDPQPTEDDESKEKRTDDIISWDADFLKVDQGTLFELILAANYLDIKGLLELTCRTVANMIKGKTPEEIRKTFNIKKDFTPAEEEQVRKENEWCEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EE6</accession>
    <accession>A0A6I8UAY8</accession>
    <name>Q29EE6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 13">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="9 13">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Uch-L5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK118ub1). Mediates bisymmetric organization of the PR-DUB complex and is involved in association with nucleosomes to mediate deubiquitination. Does not deubiquitinate monoubiquitinated histone H2B. Required to maintain the transcriptionally repressive state of homeotic genes throughout development. The PR-DUB complex has weak or no activity toward 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Polycomb group (PcG) protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, at least composed of caly/calypso, Asx and sba (MBD5/6 homolog). The PR-DUB complex associates with nucleosomes to mediate deubiquitination of histone H2AK118ub1 substrates; the association requires the positively charged C-terminal tail of caly, probably due to direct binding of DNA. Interacts (via ULD domain) with Asx (via DEUBAD domain); the interaction produces a stable heterodimer with a composite binding site for ubiquitin. Homodimerizes (via coiled-coil hinge-region between the UCH and ULD domains) to mediate assembly of 2 copies of the caly-Asx heterodimer into a bisymmetric tetramer; dimerization enhances PR-DUB association with nucleosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 12 13">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="9 13"/>
    <dbReference type="RefSeq" id="XP_001352617.1">
        <property type="nucleotide sequence ID" value="XM_001352581.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EE6"/>
    <dbReference type="SMR" id="Q29EE6"/>
    <dbReference type="FunCoup" id="Q29EE6">
        <property type="interactions" value="2524"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EE6"/>
    <dbReference type="MEROPS" id="C12.A05"/>
    <dbReference type="GeneID" id="4812103"/>
    <dbReference type="KEGG" id="dpo:4812103"/>
    <dbReference type="eggNOG" id="KOG2778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018316_0_1_1"/>
    <dbReference type="InParanoid" id="Q29EE6"/>
    <dbReference type="OMA" id="YIQYEIQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077460">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09617">
        <property type="entry name" value="Peptidase_C12_UCH37_BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.860:FF:000001">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000001">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF16">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52049">
        <property type="entry name" value="ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 9 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="14">
        <location>
            <begin position="7"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10 12">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="11 12">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001352617.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="37763" checksum="6CA26A5BA490AEA2" modified="2006-04-04" version="1">MSEGAGNWCLIESDPGVFTELIREFGCDGAQVEEIWSLDSESFKNLEPIHGLIFLFKWVQEDEPAGKVVLDKEDIFFAKQVINNACATQAILSLLMNLEHEDIKLGETLANFKEFCQCFDPYNKGLTLSNASQIRTVHNSFARQTLFELDMKNQSKDEDLYHFVGYIPIKGRLYELDGLREGPIDLGEIKPDQNWIDVVRPIIEKRMQRYSDGEIHFNLMALISDRQRIYEKQIDKLLNPAPDAMDTEEDRQTEISSLRSYIQYEVQKKKRYKVENVRRKHNYLPFIVELLKMLGETGQLMPIYEKAKQRALEREQAQRKKESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q29ER7</accession>
    <accession>A0A6I8VLW7</accession>
    <name>Q29ER7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Probable G-protein coupled receptor Mth-like 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">mthl14</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17">MV-25-SWS-2005</strain>
            <tissue evidence="16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Mth subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352496.3">
        <property type="nucleotide sequence ID" value="XM_001352460.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043630.1">
        <property type="nucleotide sequence ID" value="XM_015188144.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29ER7"/>
    <dbReference type="STRING" id="46245.Q29ER7"/>
    <dbReference type="GeneID" id="4812560"/>
    <dbReference type="KEGG" id="dpo:4812560"/>
    <dbReference type="eggNOG" id="ENOG502TB76">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035859_0_0_1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076947">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15039">
        <property type="entry name" value="7tmB3_Methuselah-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.180.11">
        <property type="entry name" value="Methuselah ectodomain, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052808">
        <property type="entry name" value="GPCR_Mth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023311">
        <property type="entry name" value="Methusela_ecto_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036272">
        <property type="entry name" value="Methuselah_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46953">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46953:SF3">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR MTH-LIKE 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63877">
        <property type="entry name" value="Methuselah ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="381"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="53"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001352496.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015043630.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="59180" checksum="95B1CFD7CED8FAEA" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q29F53</accession>
    <accession>A0A6I8UED1</accession>
    <name>Q29F53_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4814012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354167.2">
        <property type="nucleotide sequence ID" value="XM_001354131.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29F53"/>
    <dbReference type="GeneID" id="4814012"/>
    <dbReference type="KEGG" id="dpo:4814012"/>
    <dbReference type="eggNOG" id="ENOG502SDFZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2778518_0_0_1"/>
    <dbReference type="InParanoid" id="Q29F53"/>
    <dbReference type="OMA" id="PRMVKTN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072243">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29F53">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001354167.2"/>
        </source>
    </evidence>
    <sequence length="70" mass="8043" checksum="987D92208E7068B2" modified="2008-10-14" version="2">MSASNKLPHLVSSTSRYIAGRNAMQTVYWRASAGPNPRMVKTHKNFEFDRKQLAPKSVRMQNHNHSFISK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2025-06-18" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GU0</accession>
    <name>EI3G1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Eukaryotic translation initiation factor 3 subunit G-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Eukaryotic translation initiation factor 3 RNA-binding subunit 1</fullName>
            <shortName evidence="2">eIF-3 RNA-binding subunit 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Eukaryotic translation initiation factor 3 subunit 4-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eIF3g1</name>
        <name evidence="2" type="synonym">eIF3-S4</name>
        <name evidence="1" type="synonym">eIF3ga</name>
        <name type="ORF">GA21226</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-3 subunit G family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EAL32019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001354963.1">
        <property type="nucleotide sequence ID" value="XM_001354927.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GU0"/>
    <dbReference type="SMR" id="Q29GU0"/>
    <dbReference type="FunCoup" id="Q29GU0">
        <property type="interactions" value="1750"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GU0"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0274783">
        <property type="protein sequence ID" value="FBpp0273221"/>
        <property type="gene ID" value="FBgn0081214"/>
    </dbReference>
    <dbReference type="GeneID" id="4815012"/>
    <dbReference type="KEGG" id="dpo:4815012"/>
    <dbReference type="CTD" id="31243"/>
    <dbReference type="eggNOG" id="KOG0122">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034595_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GU0"/>
    <dbReference type="OMA" id="ICQGDHF"/>
    <dbReference type="PhylomeDB" id="Q29GU0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081214">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12933">
        <property type="entry name" value="eIF3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12408">
        <property type="entry name" value="RRM_eIF3G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000828">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03006">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017334">
        <property type="entry name" value="eIF3_g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024675">
        <property type="entry name" value="eIF3g_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034240">
        <property type="entry name" value="eIF3G_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12353">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037949">
        <property type="entry name" value="Transl_init_eIF-3_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000365419" description="Eukaryotic translation initiation factor 3 subunit G-1">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="188"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9W4X7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03006"/>
        </source>
    </evidence>
    <sequence length="269" mass="29932" checksum="C9AD08D012758CF5" modified="2006-04-04" version="1">MPGVETIKSSWADEVELDYGGLPPTTENVENGHKYVTEYKYNKDDKKTKVVRTYKISKQVVPKTVAKRRTWTKFGESKSDKPGPNSHTTMVSEEIIMQFLNSKEDEKANDPLLDPTKNIAKCRICNGEHWSVNCPYKGTAMDTNLMEKKAAAAASAAVDAPKSGKYVPPFLKDSQKGGLGMRGRDDTAAIRISNLSESMTEADLEELVKKIGPQSKMYLARDKNTGLCKGFAYVHFKQRKDAAAAIEILNGHGYDHLILSVEWSKPQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JT0</accession>
    <accession>A0A6I8UI14</accession>
    <name>Q29JT0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4816252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355822.2">
        <property type="nucleotide sequence ID" value="XM_001355786.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JT0"/>
    <dbReference type="FunCoup" id="Q29JT0">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="GeneID" id="4816252"/>
    <dbReference type="KEGG" id="dpo:4816252"/>
    <dbReference type="eggNOG" id="ENOG502R0XZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_883576_0_0_1"/>
    <dbReference type="InParanoid" id="Q29JT0"/>
    <dbReference type="OMA" id="DTPKFNE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072558">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JT0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355822.2"/>
        </source>
    </evidence>
    <sequence length="318" mass="36728" checksum="A6FA03E5C3879C56" modified="2008-10-14" version="2">MERLNDDCVCHILEYLPLVDQIKMCRLSPRFRELLCQIVWRKPRYRNVSVSDAMLKPLSKSDYIYFFELTSAAMEELYIWNVHSKAFDSYLGRHLMRPELSFYLRRDFSNLKELCIADENMNNSMVNTIAKNCRQLRCLSVSSAYVTGKHMISLTKLESLVARECSIETEYLSEILDQLSLTHLDLTANRHQVEKTILQSAGAKRLQRLGISDAQDYGFLLADQLPQLTTLVVSYHKVSPEDQLDMLETTRRRVEDSPGKFPKRLQSILCNVVDVEVALEQIAGLEKRHPRDTPKFSEILKIMYFLSNPVGSDKTTPP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q29P71</accession>
    <name>MYO7A_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Myosin-VIIa</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein crinkled</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ck</name>
        <name type="ORF">GA20466</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements: can function in cells as a single-molecule cargo transporter. A very slow and high-duty-ratio motor, may be suitable for tension maintenance of actin filaments. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Plays a key role in the formation of cellular projections and other actin-based functions required for embryonic and larval viability. Necessary for auditory transduction: plays a role in Johnston organ (JO) organization by functioning in scolopidial apical attachment and therefore to acoustic stimulus propagation from the antenna a2/a3 joint to transducing elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimerizes in a two headed molecule through the formation of a coiled-coil rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="sequence caution" evidence="9">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL34422" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CH379058">
        <property type="protein sequence ID" value="EAL34422.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001357353.1">
        <property type="nucleotide sequence ID" value="XM_001357317.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29P71"/>
    <dbReference type="SMR" id="Q29P71"/>
    <dbReference type="FunCoup" id="Q29P71">
        <property type="interactions" value="244"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29P71"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0280924">
        <property type="protein sequence ID" value="FBpp0279362"/>
        <property type="gene ID" value="FBgn0080461"/>
    </dbReference>
    <dbReference type="GeneID" id="4818109"/>
    <dbReference type="KEGG" id="dpo:4818109"/>
    <dbReference type="CTD" id="34882"/>
    <dbReference type="eggNOG" id="KOG4229">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_14_1_1"/>
    <dbReference type="InParanoid" id="Q29P71"/>
    <dbReference type="OMA" id="TGFQGRC"/>
    <dbReference type="PhylomeDB" id="Q29P71"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080461">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29P71">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016461">
        <property type="term" value="C:unconventional myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032027">
        <property type="term" value="F:myosin light chain binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048800">
        <property type="term" value="P:antennal morphogenesis"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008407">
        <property type="term" value="P:chaeta morphogenesis"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035317">
        <property type="term" value="P:imaginal disc-derived wing hair organization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd17092">
        <property type="entry name" value="FERM1_F1_Myosin-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17093">
        <property type="entry name" value="FERM2_F1_Myosin-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd13198">
        <property type="entry name" value="FERM_C1_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13199">
        <property type="entry name" value="FERM_C2_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01381">
        <property type="entry name" value="MYSc_Myo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000012">
        <property type="entry name" value="Myosin VIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000047">
        <property type="entry name" value="Myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000019">
        <property type="entry name" value="myosin-VIIa isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000013">
        <property type="entry name" value="Unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000036">
        <property type="entry name" value="Unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000051">
        <property type="entry name" value="Unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000075">
        <property type="entry name" value="unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000079">
        <property type="entry name" value="unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057130">
        <property type="entry name" value="Myosin_VII_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041793">
        <property type="entry name" value="MyoVII_FERM_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041794">
        <property type="entry name" value="MyoVII_FERM_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036106">
        <property type="entry name" value="MYSc_Myo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF33">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21998">
        <property type="entry name" value="FERM_C1_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24123">
        <property type="entry name" value="Myosin_VII_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0009">Actin-binding</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0505">Motor protein</keyword>
    <keyword id="KW-0518">Myosin</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0728">SH3 domain</keyword>
    <feature type="chain" id="PRO_0000306377" description="Myosin-VIIa">
        <location>
            <begin position="1"/>
            <end position="2168"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="8">
        <location>
            <begin position="63"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="IQ 1" evidence="5 9">
        <location>
            <begin position="736"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="IQ 2" evidence="5">
        <location>
            <begin position="759"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="IQ 3" evidence="5 9">
        <location>
            <begin position="805"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="IQ 4" evidence="5">
        <location>
            <begin position="828"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4 1" evidence="7">
        <location>
            <begin position="1008"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="domain" description="FERM 1" evidence="4">
        <location>
            <begin position="1250"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="1558"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4 2" evidence="7">
        <location>
            <begin position="1702"/>
            <end position="1850"/>
        </location>
    </feature>
    <feature type="domain" description="FERM 2" evidence="4">
        <location>
            <begin position="1856"/>
            <end position="2159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="2">
        <location>
            <begin position="612"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="2">
        <location>
            <begin position="712"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="156"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P08799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q9V3Z6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="9"/>
    <evidence type="ECO:0000312" key="10">
        <source>
            <dbReference type="EMBL" id="EAL34422.1"/>
        </source>
    </evidence>
    <sequence length="2168" mass="250150" checksum="E9AB5981F080B81F" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LYP7</accession>
    <accession>A0A6I8UE95</accession>
    <name>Q2LYP7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein pzg</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pzg</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354076.2">
        <property type="nucleotide sequence ID" value="XM_001354040.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LYP7"/>
    <dbReference type="FunCoup" id="Q2LYP7">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="GeneID" id="4813413"/>
    <dbReference type="KEGG" id="dpo:4813413"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_294096_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LYP7"/>
    <dbReference type="OMA" id="HQTNYFK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080555">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="263"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="166"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="564"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="922"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="578"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="635"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="682"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="730"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="943"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="966"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001354076.2"/>
        </source>
    </evidence>
    <sequence length="987" mass="103680" checksum="67B847002A59DC1C" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0H3</accession>
    <accession>A0A6I8UD01</accession>
    <name>Q2M0H3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NEDD4 family-interacting protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Ndfip</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353450.1">
        <property type="nucleotide sequence ID" value="XM_001353414.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0H3"/>
    <dbReference type="FunCoup" id="Q2M0H3">
        <property type="interactions" value="1015"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0H3"/>
    <dbReference type="GeneID" id="4812776"/>
    <dbReference type="KEGG" id="dpo:4812776"/>
    <dbReference type="eggNOG" id="KOG4812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074980_1_0_1"/>
    <dbReference type="InParanoid" id="Q2M0H3"/>
    <dbReference type="OMA" id="FASHENS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076749">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M0H3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050699">
        <property type="term" value="F:WW domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22212">
        <property type="entry name" value="NDFIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF5">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001353450.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="28473" checksum="72746C1FE2F6BD88" modified="2006-02-21" version="1">MPHNNQNSDDDQLGPPKADFSAPPPYEANVGGPQGTLQAQMPSLSLTTHVSGHEMGGPMQGGELVGGQLPQDMHGKLPTYEEVQMEKSLNGELPPAFLTLPSNQQLPPPPNPLLPPNPLRDGAAAVRAAQPALTFIAIDASDPENSLSTTDNLLGTDIMFITAFIVAFLFNWIGFLMLTCFCHTIAARYGALSGFGLSLAKWTLIVKHSTDLASHENSWLWWLICAFGFLISIRALIQYVSIKRSWRLLSASAQERLLFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NS43</accession>
    <accession>A0A6I8V824</accession>
    <name>A0A0R3NS43_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM151B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4817148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the menorin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015035802.1">
        <property type="nucleotide sequence ID" value="XM_015180316.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NS43"/>
    <dbReference type="FunCoup" id="A0A0R3NS43">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3NS43"/>
    <dbReference type="GeneID" id="4817148"/>
    <dbReference type="eggNOG" id="KOG3748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3NS43"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080193">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NS43">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019356">
        <property type="entry name" value="Menorin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184:SF6">
        <property type="entry name" value="CONSERVED PLASMA MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184">
        <property type="entry name" value="MENORIN (DENDRITIC BRANCHING PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10223">
        <property type="entry name" value="Menorin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Menorin-like" evidence="2">
        <location>
            <begin position="75"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00044953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015035802.1"/>
        </source>
    </evidence>
    <sequence length="364" mass="39659" checksum="3CC81E540244A88B" modified="2016-01-20" version="1">MLFKSIAAALDQSSLSVLCLLLALVSKINGAALNASHYQYYRVEGLEGTQVADLPLGIVVLQDQDMGKTIIRAANLTAIRWAHAVNSQQLLDEALAGDIEFIEADIVLGKLSGGDGDDMPVMAHPPANVSDLTLQAFLNQIKDFNDLHEGNEKGVKLDFKSIDVFEGSLDILDATIPSMSYPIWINADIISGPVDQNKTVPVDPNRFFAGCMRYKQAVLSIGWTTNWGAEFREGEYTKDQCDAMVETLAFNNVLSTGQGITFPVRAGIAANSEEQLHKLVAAVNETNESTLTIWSSDGDYVDIEKLRKLIFGFGVERVYLDVPEELAAKLDLGNAGNGAGTFNSLVSFSFISLCFYAFSSWLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NS93</accession>
    <accession>A0A6I8V894</accession>
    <name>A0A0R3NS93_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">Pgant5</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">MV-25-SWS-2005</strain>
            <tissue evidence="23">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Golgi apparatus membrane</location>
            <topology evidence="2 20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="20"/>
    <dbReference type="RefSeq" id="XP_015035889.1">
        <property type="nucleotide sequence ID" value="XM_015180403.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NS93"/>
    <dbReference type="SMR" id="A0A0R3NS93"/>
    <dbReference type="FunCoup" id="A0A0R3NS93">
        <property type="interactions" value="1641"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3NS93"/>
    <dbReference type="GeneID" id="4817341"/>
    <dbReference type="InParanoid" id="A0A0R3NS93"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076383">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23433">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000047">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000005">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF101">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="21">
        <location>
            <begin position="499"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_015035889.1"/>
        </source>
    </evidence>
    <sequence length="630" mass="72228" checksum="D2A2BB52FB6B7159" modified="2016-01-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NYS2</accession>
    <accession>A0A6I8V8U9</accession>
    <name>A0A0R3NYS2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Lactosylceramide 4-alpha-galactosyltransferase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC26532247</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015036235.1">
        <property type="nucleotide sequence ID" value="XM_015180749.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NYS2"/>
    <dbReference type="STRING" id="46245.A0A0R3NYS2"/>
    <dbReference type="GeneID" id="26532247"/>
    <dbReference type="KEGG" id="dpo:26532247"/>
    <dbReference type="eggNOG" id="KOG1928">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3NYS2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271234">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NYS2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035248">
        <property type="term" value="F:alpha-1,4-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006688">
        <property type="term" value="P:glycosphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007652">
        <property type="entry name" value="A1-4-GlycosylTfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051981">
        <property type="entry name" value="Glycosyltransf_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12042:SF21">
        <property type="entry name" value="ALPHA1,4-GALACTOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12042">
        <property type="entry name" value="LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04572">
        <property type="entry name" value="Gb3_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha 1,4-glycosyltransferase" evidence="7">
        <location>
            <begin position="247"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015036235.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="42918" checksum="DF4C9964B097727D" modified="2016-01-20" version="1">MILRYFTNGLLRLLFMTLIVTLIGIITLTYNWPKGADVESVPQSTVGAAEDPEPTAASSEPLASIRLDDVLLAEPRPTPGQSIFFHETSCYDVKNKNLNILKLTARQACAIESAALHNPNFQVFVLFVDRKYSHVVDPNVGNRSIQQPLFDAILSYSNVHLRRLNLWRYAAGTPMEEWLREGNLFRSRYLVSHISDFLRYLTLFRYGGLYLDMDVVVLRSMEDIPPNYTGAESDTFLAAGIMNLAASGFGHQIAASCLHDFQTNFDGNVWGQNGPEVITRVAQQICGTKNISVMQTNRKRCLGFKVFGRGAFYAVTSDKWLNFFNPHKLEETLARTKDSYAVHVWNSQSEKKPIKIGSTCAYAKYAEKNCPRAYHAAGEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P4B2</accession>
    <accession>A0A6I8VGV9</accession>
    <name>A0A0R3P4B2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein inaF-C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">inaF-C</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041021.1">
        <property type="nucleotide sequence ID" value="XM_015185535.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P4B2"/>
    <dbReference type="FunCoup" id="A0A0R3P4B2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P4B2"/>
    <dbReference type="GeneID" id="4815402"/>
    <dbReference type="KEGG" id="dpo:4815402"/>
    <dbReference type="eggNOG" id="ENOG502TCUV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A0R3P4B2"/>
    <dbReference type="OMA" id="KPTCTER"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075390">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029162">
        <property type="entry name" value="InaF-motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15018">
        <property type="entry name" value="InaF-motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015041021.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="15904" checksum="94684169B77C0360" modified="2016-01-20" version="1">MSTMSASASSSSSVCGAATSSGAGTALGAATSIANLAKIVNIIESNVRADDCDEEELEASKASTSGSSVRLASSSAPRRSTCSYVPKSPQLEEIVFAKPTCTERFARYFVIVIYLCGLCCLGFFLSIYHIFFWDSRMPPVFKGQKKPAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBL6</accession>
    <name>A0A6I8UBL6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrator complex subunit 3 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Tgi</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352904.3">
        <property type="nucleotide sequence ID" value="XM_001352868.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBL6"/>
    <dbReference type="FunCoup" id="A0A6I8UBL6">
        <property type="interactions" value="386"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812503"/>
    <dbReference type="InParanoid" id="A0A6I8UBL6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBL6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001223">
        <property type="term" value="F:transcription coactivator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006627">
        <property type="entry name" value="TDU_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028184">
        <property type="entry name" value="VGLL4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17604:SF7">
        <property type="entry name" value="TONDU-DOMAIN-CONTAINING GROWTH INHIBITOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17604">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00711">
        <property type="entry name" value="TDU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="544"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352904.3"/>
        </source>
    </evidence>
    <sequence length="594" mass="63521" checksum="1DBF0973EDFB1D77" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBV2</accession>
    <name>A0A6I8UBV2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Cysteine-rich motor neuron 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">LOC4812355</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7">MV-25-SWS-2005</strain>
            <tissue evidence="6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353005.3">
        <property type="nucleotide sequence ID" value="XM_001352969.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044080.2">
        <property type="nucleotide sequence ID" value="XM_015188594.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBV2"/>
    <dbReference type="KEGG" id="dpo:4812355"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBV2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634420" evidence="3">
        <location>
            <begin position="28"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="4">
        <location>
            <begin position="221"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="337"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001353005.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015044080.2"/>
        </source>
    </evidence>
    <sequence length="440" mass="47598" checksum="E30E46FAC48D1CDA" modified="2020-08-12" version="1">MASLLHKPGRCLHHMLIIYALIFVSSATTLDLPPLPDLCDSIKCPPESDMQCPADSSVRETLNAVDLIKWNAVDTAIHPGAGPGAGAGAGAGGGAGVGATAVAVTAVTAAESQMSYNTSLIPDEAYAQCCLSKKCVCRTCYIPDCDTAAGEVVVEVLPEAMDTPGQCCGEYQCRKEPNCTEVRETDFYWLQQCQRCQCTSGARICRQSCDEADAEKVESNAICESKNLNEYFKDGDTWRDECLECECVRGEQKCTISSCGNVDCPIERHVYLRDTCCPVCWPEGHPMPHEKQKDDADADVDVDADAEGGGDYKEDDSIEEIEEQPVPPLLPETTQRTTSSVSAELLASTTTSTTTTTTAAPSTSSTAATAASQQPCCLCSYELEPKMVEVVGENNDFSFWLMAFFVVLLLFVCLMLSSYIQHLRAKKRSYDPVSTLDHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCB0</accession>
    <name>A0A6I8UCB0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Carboxypeptidase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4813596</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353239.2">
        <property type="nucleotide sequence ID" value="XM_001353203.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCB0"/>
    <dbReference type="FunCoup" id="A0A6I8UCB0">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813596"/>
    <dbReference type="InParanoid" id="A0A6I8UCB0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF140">
        <property type="entry name" value="FI02848P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026195090" evidence="12">
        <location>
            <begin position="21"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="124"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001353239.2"/>
        </source>
    </evidence>
    <sequence length="424" mass="47588" checksum="02DD90EC00FD65E9" modified="2020-08-12" version="1">MGVLWIVLALSCLGLTEVQSNKYEGFKIYEVQTKARSAAMDQALAELAKNESYYEVFSSGSQPARIMVHPEEQVNFVQLMDGHSLSYKVLNNNVGFTLRRQFARNRILRNRFPYTGNGRLGTERFYSHGEINQFIEDLAEKHPRRVFVKTVGKSYERRLMKTVTITNGDGRRNKNIIFVDGGFHAREWISPAAVVYLIDELVNNLADNADLLLDYDWVILPVVNPDGYEYTQLSEETRMWRKTRKPSSPDCIGTDPNRNFDFHWNEEGASEDPCAQTYAGSKAFSEPEAVVVSDLIHSYADRGKMYLTLHSFGPYILYPWGWSSDLPDTVDDLHEVAKAGFDAILEATGTLYEIGSSTNVLYVAAGASDDYAFNAGFPISFTMELPPGGTGFDPPASAIDDMVKETWVGIAAMARKVVQKYPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDJ6</accession>
    <name>A0A6I8UDJ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein dpr20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dpr20</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353671.3">
        <property type="nucleotide sequence ID" value="XM_001353635.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDJ6"/>
    <dbReference type="FunCoup" id="A0A6I8UDJ6">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813263"/>
    <dbReference type="InParanoid" id="A0A6I8UDJ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001278">
        <property type="entry name" value="Defective proboscis extension response"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001606">
        <property type="entry name" value="uncharacterized protein LOC108091111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF12">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 14, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="353"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="465"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353671.3"/>
        </source>
    </evidence>
    <sequence length="611" mass="65596" checksum="6DDAA2F925BF9BAE" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDJ9</accession>
    <name>A0A6I8UDJ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Serine protease 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Jon65Ai</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353840.2">
        <property type="nucleotide sequence ID" value="XM_001353804.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDJ9"/>
    <dbReference type="KEGG" id="dpo:4813468"/>
    <dbReference type="InParanoid" id="A0A6I8UDJ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDJ9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000025">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000043">
        <property type="entry name" value="serine proteases 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF98">
        <property type="entry name" value="FI18310P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 11" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026134991" evidence="8">
        <location>
            <begin position="18"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="38"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353840.2"/>
        </source>
    </evidence>
    <sequence length="262" mass="28406" checksum="36FDC7E19A0984E9" modified="2020-08-12" version="1">MKLLAVLLLGVIASVSAFEKPVFWKDVPVGKGSIEGRITMGYPAHEGKVPYIVGLGFSKNGGGTWCGGSIIGNTWVMTAKHCTDGMESVTIYYGALWRLQAQYTHWVGRNDFIEHGSGDISLIRTPHVDFWSLVNKVELPSYNDRYNNYQGWWALVSGWGKTSDDGGVSEYLNCVDVQIGPNSVCESYYGSFSGDLICIPTPENKGTCSGDSGGPLVIHDGNRQVGIVSFGSSAGCLSNGPKGMVRVTSYLDWIRDHTGISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDT1</accession>
    <name>A0A6I8UDT1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353810.2">
        <property type="nucleotide sequence ID" value="XM_001353774.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDT1"/>
    <dbReference type="KEGG" id="dpo:4813156"/>
    <dbReference type="InParanoid" id="A0A6I8UDT1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031754">
        <property type="entry name" value="DUF4736"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15883">
        <property type="entry name" value="DUF4736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353810.2"/>
        </source>
    </evidence>
    <sequence length="210" mass="24523" checksum="56FC8C68F60B7911" modified="2020-08-12" version="1">MDLWKGLWRQCVNFFSYNYPCIAVYTSAGAVVTAYLHYHLRIHSLDLMYWKNVAESFSKEIDTFQLGMICIIFAINGVYVFVLLTVYLCPALERDDVDMTLLEIKDRYARYILLRLIARLDRIQGGLSNRRQSLCLQDYTRAHSSMAKAVAVFRKDARKLILPSESEIMLPIEDIYHVAEEDERQLRRAGYYKLGIDISDETVKKLYNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF91</accession>
    <name>A0A6I8UF91_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA (cytosine(34)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.203</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Nsun2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.203" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001354507.4">
        <property type="nucleotide sequence ID" value="XM_001354471.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UF91"/>
    <dbReference type="FunCoup" id="A0A6I8UF91">
        <property type="interactions" value="2630"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815355"/>
    <dbReference type="InParanoid" id="A0A6I8UF91"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057285">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057286">
        <property type="entry name" value="PUA_NSUN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023270">
        <property type="entry name" value="RCMT_NCL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808">
        <property type="entry name" value="NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808:SF1">
        <property type="entry name" value="RNA CYTOSINE-C(5)-METHYLTRANSFERASE NSUN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25376">
        <property type="entry name" value="Pre-PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25378">
        <property type="entry name" value="PUA_NSUN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02011">
        <property type="entry name" value="RCMTNCL1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="13">
        <location>
            <begin position="61"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="702"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="702"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="184"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001354507.4"/>
        </source>
    </evidence>
    <sequence length="729" mass="82431" checksum="D59541C86BACE77B" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UG69</accession>
    <name>A0A6I8UG69_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Rbm13</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MAK16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354987.3">
        <property type="nucleotide sequence ID" value="XM_001354951.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UG69"/>
    <dbReference type="FunCoup" id="A0A6I8UG69">
        <property type="interactions" value="2178"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814882"/>
    <dbReference type="InParanoid" id="A0A6I8UG69"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UG69">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000001">
        <property type="entry name" value="Protein MAK16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="6">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="232"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="232"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001354987.3"/>
        </source>
    </evidence>
    <sequence length="334" mass="39289" checksum="DE8D8B125102E058" modified="2020-08-12" version="1">MQHDDVVWSIINKSFCSHKVKTDTRTFCRHEYNLTGLCTRRTCPLANSQYATVREENGIIYLFMKTAERAHMPAKLWERIKLSRNFNKAIEQINENLVFWPKYIISKNKQRFLKITQYLMRMRQLKLRRQKLIVPLSTKIERRETRREEKALVAAKIDNHIEKALLERLKHGTYRDIYNFSQTAFNKALEAERIEDEQDEEEDEEEELEVEREMDVDGEAAELHRDLLSEEFVEAETDDEEDDDDSQSESGQREEVEMDSDFGHSDDDDDDDADADDIEDIKVPAKKTKSKKTSDTADAAAATSSTKRRRAKPKLELEYEYEVETGAEREKVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGT2</accession>
    <name>A0A6I8UGT2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4815633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355292.3">
        <property type="nucleotide sequence ID" value="XM_001355256.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGT2"/>
    <dbReference type="KEGG" id="dpo:4815633"/>
    <dbReference type="InParanoid" id="A0A6I8UGT2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053225">
        <property type="entry name" value="Acyl-CoA_N-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041506">
        <property type="entry name" value="DUF5645"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="GCN5-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20958:SF10">
        <property type="entry name" value="GH05617P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20958">
        <property type="entry name" value="GLYCINE N-ACYLTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18713">
        <property type="entry name" value="DUF5645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5645" evidence="2">
        <location>
            <begin position="11"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="GCN5-related N-acetyltransferase Rv2170-like" evidence="1">
        <location>
            <begin position="214"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355292.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="34878" checksum="9047DE87B13EC2D0" modified="2020-08-12" version="1">MGINADKDIDILRPLSDNEVDELLGLYERKYGPQNFHYLLLYNQRKWDCQLAAAGIARNDPAYISLRKQFYTHRQGDFRRFGTYVSLHRDIVQSVSFFSWQPEGARELFECLERTKRIEWTRGALLTNVDLAFCERVKALAAQRGVTTINARQCFGMVLSHPRACTAEVPELLSEFELRPLRDEDAPMVHAIWPNKGEGSLAYLRALIKFNKTIGACRSDTGELIAWIFQNDFSGLGMLQVLPKAERRGLGGLLAAAITRRIARVEQVTLTAWIVATNWRSEALLKRIGYHQDVINEWIKLEPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH19</accession>
    <name>A0A6I8UH19_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Putative inositol monophosphatase 3</fullName>
            <ecNumber evidence="6">3.1.3.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inositol-1(or 4)-monophosphatase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Myo-inositol monophosphatase A3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4815471</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001355274.4">
        <property type="nucleotide sequence ID" value="XM_001355238.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UH19"/>
    <dbReference type="FunCoup" id="A0A6I8UH19">
        <property type="interactions" value="1469"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815471"/>
    <dbReference type="InParanoid" id="A0A6I8UH19"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008254">
        <property type="term" value="F:3'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052834">
        <property type="term" value="F:inositol monophosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01640">
        <property type="entry name" value="IPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000012">
        <property type="entry name" value="Blast:Putative inositol monophosphatase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000007">
        <property type="entry name" value="Blast:Putative inositol monophosphatase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF4">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001355274.4"/>
        </source>
    </evidence>
    <sequence length="361" mass="39997" checksum="0C43279654DB616C" modified="2020-08-12" version="1">MCLTSSESSSKMNGRSIRINRVPATIFAILLTIVLVYFLNFHQEERPAIYGKLRSDNPNRVNLRKMLIAAIQASQRGGLEVLDVARSRQLKERSKGQTDEGVNDPFTDADGRSHCVMKQGLQRIFPRVRIFSEEDKEHCKESHSYDLDPTVLHETAQVPDVSVNAQDVTVWVDPLDATKEFTEELYEYVTTMVCVAVAGRPVIGVIHSPFNGQTAWAWVGNSMSEYLAGLHPPHGQENELPIITVSRSHTAGAKDLARGIFGEQVNLLTAAGAGYKVLQVVANNATAYLHTSKIKKWDICAGDAILHALGGTMTTLNDQQIRYGPDESPVNTEGLLATLEKHDKYMDQLVKYRTAHNGQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UI38</accession>
    <name>A0A6I8UI38_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein phosphatase inhibitor 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein phosphatase inhibitor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355883.2">
        <property type="nucleotide sequence ID" value="XM_001355847.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UI38"/>
    <dbReference type="FunCoup" id="A0A6I8UI38">
        <property type="interactions" value="270"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816360"/>
    <dbReference type="InParanoid" id="A0A6I8UI38"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UI38">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1050">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001355883.2"/>
        </source>
    </evidence>
    <sequence length="262" mass="29072" checksum="64D72B774E064B0F" modified="2020-08-12" version="1">MNPEDVKGILKVGSGGAASQPPAAGQKTAKFDELNVLETFHPEGKTYGHMVIDEPKTPFVFESDLPKELDTAELIEKLRRTSLLTTPCFGMDQDSDDSSDDEEFPETVEEKVRRLEFERRRKLHYREFFSVPLARRLIAEEFGDMSDSEHSYKSETSACVPETCPKDTYDTMMGEDDQARTSHSLADAGSEDGGYFIEPEPGFDPSHPCYQKLMAEIKRPANAEADKALDTASHGKPVRVPDPSSETDLSAELKKSQSGSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIT0</accession>
    <name>A0A6I8UIT0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptide chain release factor 1-like, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mRF1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356138.2">
        <property type="nucleotide sequence ID" value="XM_001356102.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIT0"/>
    <dbReference type="FunCoup" id="A0A6I8UIT0">
        <property type="interactions" value="1949"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816564"/>
    <dbReference type="InParanoid" id="A0A6I8UIT0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIT0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="6">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="52"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001356138.2"/>
        </source>
    </evidence>
    <sequence length="392" mass="44511" checksum="0E939988522DE7AD" modified="2020-08-12" version="1">MLLRKLLPVRCGYLARRWASSDPLSLQNEKLQSYLESLRQEYYGLRVNATGNSRAYERLSQLEGVVNALEQRRVLERNITSAKDIEQEKDEDMRKLMEEENEVYADLLSKQEHTMLQELLALADDEVYPALIFGVNAGAGGQEAMLFAQELFEMYTSYFEHMGWEYEEFARETTDIGGLRHANVMVNGADAFHWLRFEAGVHRVQRVPATEKAGRMHTSTASITVIPRPADVHVHIAEKDLKIETKRASGAGGQHVNTTDSAVRIVHLPTGLAVEAQSERSQLKNRELALKRLQARLVQQQLESSEASKQATKKAQQGSLNRNEKIRTYNFVQDRVTDHRIQGGTLHNLDGFLKGGESLSGLVEKLQLEHRRSRLRDLLEAWQPPQKAANKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIW0</accession>
    <name>A0A6I8UIW0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein CDV3 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDV3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356191.3">
        <property type="nucleotide sequence ID" value="XM_001356155.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIW0"/>
    <dbReference type="FunCoup" id="A0A6I8UIW0">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816370"/>
    <dbReference type="InParanoid" id="A0A6I8UIW0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026806">
        <property type="entry name" value="CDV3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16284">
        <property type="entry name" value="PROTEIN CDV3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16284:SF13">
        <property type="entry name" value="PROTEIN CDV3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15359">
        <property type="entry name" value="CDV3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356191.3"/>
        </source>
    </evidence>
    <sequence length="232" mass="25855" checksum="C233D2498C3C2E75" modified="2020-08-12" version="1">MSNLEDFFSKKDNKKSKRSAEYLAAEELYKTLEESTKYSSEDNSNLEDAFQSEASGTDSKGASASLKFGIRFSDEAFSEEDEWSDFTEENRRQFTYVPRTLSMALTSPVPAPAPFAGDDLKEEPEQLDTGGDGVDVGSKDGERALATTCPWQKSEQDSQGQSDIKANTPVVKKQIYIPPALRQSQGDFNKRKAQPLQRKAPSTMPFKPQAPDLNSLEYFPSLSDSKILKRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK40</accession>
    <name>A0A6I8UK40_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Disks large homolog 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Dlg5</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356808.4">
        <property type="nucleotide sequence ID" value="XM_001356772.4"/>
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        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816824"/>
    <dbReference type="InParanoid" id="A0A6I8UK40"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035331">
        <property type="term" value="P:negative regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06767">
        <property type="entry name" value="PDZ3_DLG5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11860">
        <property type="entry name" value="SH3_DLG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000130">
        <property type="entry name" value="Discs large 5, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000365">
        <property type="entry name" value="Discs large 5, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001678">
        <property type="entry name" value="Disks large 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035537">
        <property type="entry name" value="DLG5_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053004">
        <property type="entry name" value="MAGUK_Signaling_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46360">
        <property type="entry name" value="DISKS LARGE HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46360:SF1">
        <property type="entry name" value="DISKS LARGE HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="446"/>
            <end position="525"/>
        </location>
    </feature>
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        <location>
            <begin position="1344"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="1557"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="3">
        <location>
            <begin position="1831"/>
            <end position="1960"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="707"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1122"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1218"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1436"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1479"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="191"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
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        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="629"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="712"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1168"/>
            <end position="1178"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1232"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1480"/>
            <end position="1506"/>
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    </feature>
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        <location>
            <begin position="1507"/>
            <end position="1533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001356808.4"/>
        </source>
    </evidence>
    <sequence length="1974" mass="215618" checksum="049E6BA691EBE0CC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKC6</accession>
    <name>A0A6I8UKC6_DROPS</name>
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        <submittedName>
            <fullName evidence="11">Facilitated trehalose transporter Tret1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4816775</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
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    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
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            <begin position="62"/>
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            <begin position="94"/>
            <end position="113"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="140"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
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            <begin position="23"/>
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    </feature>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001356768.2"/>
        </source>
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    <sequence length="466" mass="51901" checksum="DB6EED6DA662A38C" modified="2020-08-12" version="1">MLGRLFARPNCLLNRRNRYQLLTTLIINLISVSHGIGIGWLSPTLRKLQSNSPIGFEVKSEFEISWVGSMLGMGSVTGNILIGTHLGRLGSKRCLLFLAIPHSCLWFLVYFAQSVEYLYVGRLLAGITGGGMYIVHPIFLSEISDANIRGTFSAMVMLSVNVGVLGGYIVGTHLAYYDIPWMVLVLPLCYFISVLLFIRESPMHLIRIGKYAAAERSFRYYKNIKDGDNINDQNRSMEEFEHMKIALTKGDDKKDVVTFKDFVSRPALRAYGPALVLLIANQFSGLFTMVNYMSDIFSKSGSTMDPDTSTIIIGAVQIMGSYVTTLLCDICGRKLLMLVSTAGVAISLISFGFFTKYAQSYDLSEWSWVPLVLMSMDIFLGNIGLVGCFFVCLVEIFPLKIRAKAASIAIVVCSSFVFLMLNIFPLCMRHWGISATMWSCGGVSAFSFIFFAYFLKETKGKSMLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKQ3</accession>
    <name>A0A6I8UKQ3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Paramyosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4817609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357011.3">
        <property type="nucleotide sequence ID" value="XM_001356975.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKQ3"/>
    <dbReference type="FunCoup" id="A0A6I8UKQ3">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817609"/>
    <dbReference type="InParanoid" id="A0A6I8UKQ3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKQ3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39960">
        <property type="entry name" value="LD34147P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39960:SF1">
        <property type="entry name" value="LD34147P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026046756" description="Paramyosin" evidence="1">
        <location>
            <begin position="28"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001357011.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="59744" checksum="EE9E93B3A791F7C3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL63</accession>
    <name>A0A6I8UL63_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Activator of 90 kDa heat shock protein ATPase homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC4818064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357268.3">
        <property type="nucleotide sequence ID" value="XM_001357232.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235791.1">
        <property type="nucleotide sequence ID" value="XM_033379900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL63"/>
    <dbReference type="KEGG" id="dpo:4818064"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UL63">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08892">
        <property type="entry name" value="SRPBCC_Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="ASHA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF22">
        <property type="entry name" value="LD43819P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="2">
        <location>
            <begin position="29"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357268.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033235791.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="40324" checksum="4891D36960513DE6" modified="2020-08-12" version="1">MAKWGEGDPRWIVEERPDATNVNNWHWTEKNATPWSKERLHQLFKDFKIDQSDIECVVEAVDKCQGEATVNNRKGKLIYFYEWELVLKWSGQLLKNSKLSHKGKLTIPNLSEENDLEDVEITVTIDESNDESETLKQFMYNVGRDSIRKQLGVYIKELKEEYSKNLILPKKGDEGGNSINNASLNDANNARNAAQKAAAAAAAAVATPKASDSSKVGCKLDVRTLSMTEEFHCNANDLYNALTKPDMVSAFTRAPAKVDPVRGGEFVLYGGNVLGKFEELIPEKKIQQSWRLKNWSSGHFSNVLIELEETSNSTMMTLKQTGIPASEYDAMKTNWHRYYWHSIKQTFGFGTSIADSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL93</accession>
    <name>A0A6I8UL93_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein nemuri</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nur</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357309.3">
        <property type="nucleotide sequence ID" value="XM_001357273.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL93"/>
    <dbReference type="FunCoup" id="A0A6I8UL93">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817858"/>
    <dbReference type="InParanoid" id="A0A6I8UL93"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026112303" evidence="2">
        <location>
            <begin position="24"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357309.3"/>
        </source>
    </evidence>
    <sequence length="165" mass="18280" checksum="AB4CCBC1013BA010" modified="2020-08-12" version="1">MSAKYALIFALAALCCLVATTEAAAQRSRVLSARRGSELVERTEGKEETEVAAQEQEQEEQQPEDGNQLEGRSEDDVAQAENKLETTDKEKDTIVRPNKDDARARRIVRAGRRRGGRRGGRRGGRRGGRRGGRRGGRRRGGRRGSRAGARRRKSVKRRSGKGNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULW4</accession>
    <name>A0A6I8ULW4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Cog7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357618.2">
        <property type="nucleotide sequence ID" value="XM_001357581.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULW4"/>
    <dbReference type="FunCoup" id="A0A6I8ULW4">
        <property type="interactions" value="875"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800274"/>
    <dbReference type="InParanoid" id="A0A6I8ULW4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019335">
        <property type="entry name" value="COG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21443">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21443:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10191">
        <property type="entry name" value="COG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="82"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001357618.2"/>
        </source>
    </evidence>
    <sequence length="742" mass="84038" checksum="01B31DFF7B3C5F3A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UM89</accession>
    <name>A0A6I8UM89_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCR4-NOT transcription complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Not10</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CNOT10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357791.3">
        <property type="nucleotide sequence ID" value="XM_001357754.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UM89"/>
    <dbReference type="FunCoup" id="A0A6I8UM89">
        <property type="interactions" value="845"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800526"/>
    <dbReference type="InParanoid" id="A0A6I8UM89"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039740">
        <property type="entry name" value="CNOT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12979">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12979:SF5">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357791.3"/>
        </source>
    </evidence>
    <sequence length="641" mass="70491" checksum="7D67B5A44D6901A9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UMS5</accession>
    <name>A0A6I8UMS5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Chymotrypsin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4800762</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357978.3">
        <property type="nucleotide sequence ID" value="XM_001357941.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UMS5"/>
    <dbReference type="FunCoup" id="A0A6I8UMS5">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800762"/>
    <dbReference type="InParanoid" id="A0A6I8UMS5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000047">
        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF96">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026355392" evidence="10">
        <location>
            <begin position="26"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="48"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001357978.3"/>
        </source>
    </evidence>
    <sequence length="278" mass="30036" checksum="7B887D9BFDAD2698" modified="2020-08-12" version="1">MSCRIRILSSVVCLLLLLLVSFTQAELRRRAFTGASGATAANKFSSRVVGGEVSDVKAAPYQVSLQNSYGNHFCGGVIIHDQYVVTAASCLAGLRKNNVKVVTTTYNDWGLDGWEYAVEEIIPHCNFDQPLYHNDIALIKTKTLFAYDEVTQNITVAPLEDLVEGEKLTMYGWGSTSAAGDFEWELRQVDLTYVLTEKCNATYGGTADLDLGHLCAVGRVGAGACHGDAGGPLVDSQGRLVGVGNWGVPCGHGFPDIFARLSFYYSWIQSTINGCSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNN5</accession>
    <name>A0A6I8UNN5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein TwdlD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TwdlD</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358433.2">
        <property type="nucleotide sequence ID" value="XM_001358396.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNN5"/>
    <dbReference type="FunCoup" id="A0A6I8UNN5">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:5687544"/>
    <dbReference type="InParanoid" id="A0A6I8UNN5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026271327" evidence="1">
        <location>
            <begin position="17"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="2">
        <location>
            <begin position="77"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358433.2"/>
        </source>
    </evidence>
    <sequence length="254" mass="27874" checksum="D5B2208F1B2E6C1F" modified="2020-08-12" version="1">MRVFILLCLFAASCSADKLGYNYQPVAHADEGLSFLPGSGQIPVEIQQQLQQVQSGEAVLSQPIEAPIIPQAVPLVEEFQKEFYSYAAPEEQFDEGVNNQQIASSLKKNLRVVFIRTPENQGFERAALQLAKQSAQQETAIYVLSKQADVANLAKQLNALKSTSSNKPEVHFVKYRTPEDAANAQLAIQNQYNQLPGVSRISNEGRAPVLNFASPASQSIPASVSRSPSSEYLPANVVAGQDYLPPTLRRFRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQE5</accession>
    <name>A0A6I8UQE5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Modular serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">modSP</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359065.3">
        <property type="nucleotide sequence ID" value="XM_001359028.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQE5"/>
    <dbReference type="FunCoup" id="A0A6I8UQE5">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802072"/>
    <dbReference type="InParanoid" id="A0A6I8UQE5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UQE5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026047548" evidence="4">
        <location>
            <begin position="27"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="223"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="301"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="371"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359065.3"/>
        </source>
    </evidence>
    <sequence length="629" mass="69645" checksum="39C311B5E5E9E61E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQK1</accession>
    <name>A0A6I8UQK1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="4">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4802179</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="4"/>
    <dbReference type="RefSeq" id="XP_001359148.2">
        <property type="nucleotide sequence ID" value="XM_001359111.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQK1"/>
    <dbReference type="FunCoup" id="A0A6I8UQK1">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802179"/>
    <dbReference type="InParanoid" id="A0A6I8UQK1"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00948">
        <property type="entry name" value="FBP_aldolase_I_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000140">
        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033379">
        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359148.2"/>
        </source>
    </evidence>
    <sequence length="364" mass="39874" checksum="9F4F73D5C526F601" modified="2020-08-12" version="1">MTTHFYYPDRELQDELSCIAKGLVAPGKGILAVDETSSQMGKRLELIGVENTEENRRLYRQILFTTDAKIAENISGVIFPPEALHQKTDDSVPFAEVLRKKGILTGIKVDKQFVALFGSEDEFTTQGLDDLAARCSQYKKEGCSFAKWRCVLKISKNTPSHQAILENANVLARYAATCQSQGLVPIINPDILAVGDHDLDRCQKVAETVLAAVYKSLNDHHIFLEGTLLQPSIVTPGMQSQKSTLPADIGLCTVLAIRRTVPPAVPGVMFCSGNQSEEEATVNLNAINNVPLCKPWAMTFAFDRALQSSVLRTWGGRKDNVVNAQNELIKRCKANSLASIGKYIPGSVEGSAGTEKLIFDALEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URF0</accession>
    <name>A0A6I8URF0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Organic cation transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4802872</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359703.2">
        <property type="nucleotide sequence ID" value="XM_001359666.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URF0"/>
    <dbReference type="KEGG" id="dpo:4802872"/>
    <dbReference type="InParanoid" id="A0A6I8URF0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17317">
        <property type="entry name" value="MFS_SLC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="45"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001359703.2"/>
        </source>
    </evidence>
    <sequence length="555" mass="61013" checksum="892F9D7232F97FC9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URJ9</accession>
    <name>A0A6I8URJ9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Solute carrier family 35 member B1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">meigo</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359656.3">
        <property type="nucleotide sequence ID" value="XM_001359619.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URJ9"/>
    <dbReference type="FunCoup" id="A0A6I8URJ9">
        <property type="interactions" value="929"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802813"/>
    <dbReference type="InParanoid" id="A0A6I8URJ9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="278"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="327"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001359656.3"/>
        </source>
    </evidence>
    <sequence length="343" mass="37769" checksum="F1D00C0CA25DBF51" modified="2020-08-12" version="1">MNVPERSRFVIYAVGIFVCYFLYGIVQEKLTRGRYGEQVQADGSVGERFTFALALVWVQCVCNYIFAKVLLTVKPQKEDTTHTGSYAACSLTYLLAMVSTTMAMRWVPYPTAVVGKSAKPIPVMILGVLIGRKSYSWTRYACVLSIVLGVILFMYKEGKVANLPAETTLLGEVLLFLSLSMDGLTGAVQERIRAASSPSGQQMMKSMNFWSTLMLGFAMLLTGEIKESLHFAIRHPEVWTHLSLLSLCGALGQFFIFLTVANFGPLACSVVTTTRKFFTVLCSVLLFGNVLIARQWLGAVLVFAALFVDMLYGKKQSPAAPVSSKKHPSDGGKLSEEKKDLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URK9</accession>
    <name>A0A6I8URK9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Myotubularin-related protein 13 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Sbf</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359669.2">
        <property type="nucleotide sequence ID" value="XM_001359632.5"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8URK9">
        <property type="interactions" value="1798"/>
    </dbReference>
    <dbReference type="GeneID" id="4802833"/>
    <dbReference type="KEGG" id="dpo:4802833"/>
    <dbReference type="InParanoid" id="A0A6I8URK9"/>
    <dbReference type="OMA" id="NCINCIF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079966">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8URK9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13208">
        <property type="entry name" value="PH-GRAM_MTMR5_MTMR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01235">
        <property type="entry name" value="PH_Sbf1_hMTMR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14534">
        <property type="entry name" value="PTP-MTMR5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.200:FF:000004">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000006">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022096">
        <property type="entry name" value="SBF1/SBF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF109">
        <property type="entry name" value="SET DOMAIN BINDING FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12335">
        <property type="entry name" value="SBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="6"/>
            <end position="461"/>
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        <location>
            <begin position="1139"/>
            <end position="1652"/>
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    </feature>
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            <begin position="1775"/>
            <end position="1823"/>
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            <begin position="1874"/>
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            <end position="1329"/>
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            <begin position="1313"/>
            <end position="1325"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001359669.2"/>
        </source>
    </evidence>
    <sequence length="1978" mass="222779" checksum="0ACFA14A5BC90282" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URV7</accession>
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        <submittedName>
            <fullName evidence="15">Transcription factor Ouib</fullName>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="15">MV-25-SWS-2005</strain>
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        </source>
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    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
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    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
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            <end position="79"/>
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        <location>
            <position position="6"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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        <location>
            <position position="9"/>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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        <location>
            <position position="52"/>
        </location>
        <ligand>
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    <sequence length="403" mass="45857" checksum="B89BF03681F5E37F" modified="2020-08-12" version="1">MLKNVCRICASNTDDSKALKLFMSSTRELVQQINLIAGILLQFDPDLPDWICERCQTALQGAIEFRDQCLSSQDKFKRFDGTLTEGSRPASTHLFCLRRSTRSQKQLDAATELKNLPSPIEVMIKVENLSNGHEEDDGVDHLDTSNETDLDFVIKEFPLSEDDDVPGSAAKIRRGRRKRCSSEKRQKPALTVFFCDQCGTNITGKTSFDRHLRKHSGVRPFQCQLCPARFLSAGELKGHQVMHTGDRNFPCRYCDRSYVNYSGRLRHERTHTNDRPFVCSQCSKGFTNSYILKNHMLIHTGERLFRCELCQRSFSRPTHLKTHYRSNTHKHNMEKAKAEKQQLADCLQMALPQQAEQTEFLVEQQQPALGFTIDVPLPLEQASSLDLAAQQLLGPTTLVFSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URY5</accession>
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        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
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        </citation>
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        </source>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="14">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="286"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001359934.2"/>
        </source>
    </evidence>
    <sequence length="375" mass="43258" checksum="84E42FC770DA3B30" modified="2020-08-12" version="1">MNQFCRTCGKTIVTENSLRIFSREGRQLLLCVRTIANCWLQNDPEFPCYMCADCQKLLRKVCRFRRRCAKIEKYLTRRKAKLTLGEGPASVSLQLPAQQVVAADDPLGIDELLVANEIKEEDELVDEQKAYEAERYLGKHLEDCSYEDDHDQADDYLPLQAEYGESETEAVHATAKSDTEPAVTGSNKTKGKMKANKQTMRLGRKLIHVKVIDDANPKRIVDRDSQIAKPCICEHCGRQFRDTSNLNVHLLRHTGTKPFECEICHQKCYTLHLLRRHQLKHTEGPYACTFCGLKYSTNSSRVRHEREACKKGRAPQSKWEIIKKGERTFHCEVCDLWFLRAGNFTQHIKSTSHIENERRKKRKTKTVQQKSSINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US95</accession>
    <name>A0A6I8US95_DROPS</name>
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        <recommendedName>
            <fullName evidence="2">Transgelin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Mp20</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calponin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001360091.3"/>
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        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="GeneID" id="4803340"/>
    <dbReference type="KEGG" id="dpo:4803340"/>
    <dbReference type="InParanoid" id="A0A6I8US95"/>
    <dbReference type="OMA" id="VEWITVQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078366">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21207">
        <property type="entry name" value="CH_dMP20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transgelin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF24">
        <property type="entry name" value="MUSCLE-SPECIFIC PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
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    </dbReference>
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        <property type="entry name" value="CH"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
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        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
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        <property type="entry name" value="CALPONIN_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="17"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001360128.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="20176" checksum="0A60CB3AE33CA578" modified="2020-08-12" version="1">MSLERAVRAKIAGKRNPEMDKEAQEWVESIIGEKFPAGQAYEDVLKDGQVLCKLINILSPNAVPKVNSSGGQFKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFALGRATYKHDDFKGPFLGPKPADECKRDFTDEQLKAGQTIVGLQAGSNKGATQAGQNLGAGRKILLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USE4</accession>
    <name>A0A6I8USE4_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 14 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4803122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359917.4">
        <property type="nucleotide sequence ID" value="XM_001359880.4"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A6I8USE4">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803122"/>
    <dbReference type="InParanoid" id="A0A6I8USE4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079727">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8USE4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039190">
        <property type="entry name" value="TTC14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184:SF9">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="336"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="377"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="428"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="532"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="603"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="683"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="535"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="689"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="736"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="848"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="859"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="872"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="896"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="910"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="946"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359917.4"/>
        </source>
    </evidence>
    <sequence length="969" mass="110131" checksum="A6AF5656D01194FA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USF7</accession>
    <name>A0A6I8USF7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">J domain-containing protein CG6693</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4803146</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359936.1">
        <property type="nucleotide sequence ID" value="XM_001359899.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USF7"/>
    <dbReference type="SMR" id="A0A6I8USF7"/>
    <dbReference type="FunCoup" id="A0A6I8USF7">
        <property type="interactions" value="2663"/>
    </dbReference>
    <dbReference type="GeneID" id="4803146"/>
    <dbReference type="KEGG" id="dpo:4803146"/>
    <dbReference type="InParanoid" id="A0A6I8USF7"/>
    <dbReference type="OMA" id="WLDLWSK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079782">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000035">
        <property type="entry name" value="DnaJ homolog subfamily C member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056453">
        <property type="entry name" value="HTH_DNAJC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23302">
        <property type="entry name" value="HTH_DNAJC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="15"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359936.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="35137" checksum="6D67717D87F8FA55" modified="2020-08-12" version="1">MTTLELCEKYFETRDVYKLMSLQKDAKEKEIKKAYHKLSLLVHPDRVPEDQKEESTEKFKVLSKIYQVLTDTQKRALYDEQGVIDDDDDAEAKLTSWLELWSKIFKPISEEDINNYEKEYVDSELERTDIKKAYLGGKGCINYLMNHIPFMKVEDEPRIKKIVEAMIAAEEVPEYKIFTEEPSAKRKKRHNKYAREFKEATVIKERIQRRQQQKDEEDLEASGGSLQQMILARKSQRESNYNSLMDRLLEKYGGEDESQSVDFGDFERKKKSKAKKSSKPNPKEKLKGVRTGRVEKAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTI5</accession>
    <name>A0A6I8UTI5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bromodomain testis-specific protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tbrd-3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001360386.2">
        <property type="nucleotide sequence ID" value="XM_001360349.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTI5"/>
    <dbReference type="GeneID" id="4803712"/>
    <dbReference type="KEGG" id="dpo:4803712"/>
    <dbReference type="InParanoid" id="A0A6I8UTI5"/>
    <dbReference type="OMA" id="WVFYEPL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075847">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="CDD" id="cd05498">
        <property type="entry name" value="Bromo_Brdt_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043509">
        <property type="entry name" value="Bromo_Brdt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="36"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="6">
        <location>
            <begin position="174"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001360386.2"/>
        </source>
    </evidence>
    <sequence length="265" mass="30565" checksum="A32274F058FD3063" modified="2020-08-12" version="1">MSDRNRNCNRNRNRNPEPFPESNACKAIIRKLFSSQYKKLAWIFYEPIDAPYLGLHDYHKIVKKPMDLNSIRTRLQAGLYVNADEFVRDVRLMFDNTYLYTTPDHLCHQMAKKLQAIFEAMFFEIASTDSSDTITETDVSSMFPIATIPDAPINPHKDGGVKMTRARRQQLQSRSANEAAVRPWTAKENNHLGDRLQELKGEVLHRVIHIIKEMENIPIINGGMEFNLESLKTQTKCSIVRYLASKDHSSGRRTRSKIYLSTSCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUD4</accession>
    <name>A0A6I8UUD4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein lethal(2)denticleless</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">l(2)dtl</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat cdt2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361076.2">
        <property type="nucleotide sequence ID" value="XM_001361039.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUD4"/>
    <dbReference type="FunCoup" id="A0A6I8UUD4">
        <property type="interactions" value="805"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804528"/>
    <dbReference type="InParanoid" id="A0A6I8UUD4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001593">
        <property type="entry name" value="protein lethal(2)denticleless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051865">
        <property type="entry name" value="WD-repeat_CDT2_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852:SF0">
        <property type="entry name" value="DENTICLELESS PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852">
        <property type="entry name" value="LETHAL 2 DENTICLELESS PROTEIN RETINOIC ACID-REGULATED NUCLEAR MATRIX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="141"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="360"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="476"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="677"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="484"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="713"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="758"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001361076.2"/>
        </source>
    </evidence>
    <sequence length="772" mass="84282" checksum="4C2B60E1E6CA4877" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV36</accession>
    <name>A0A6I8UV36_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Complex III subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ox</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UQCR10/QCR9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361263.1">
        <property type="nucleotide sequence ID" value="XM_001361226.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV36"/>
    <dbReference type="FunCoup" id="A0A6I8UV36">
        <property type="interactions" value="640"/>
    </dbReference>
    <dbReference type="GeneID" id="4804752"/>
    <dbReference type="KEGG" id="dpo:4804752"/>
    <dbReference type="InParanoid" id="A0A6I8UV36"/>
    <dbReference type="OMA" id="IKHKYEV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081290">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.260:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.260">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008027">
        <property type="entry name" value="QCR9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036656">
        <property type="entry name" value="QCR9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX, SUBUNIT X"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UCR_UQCRX_QCR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81514">
        <property type="entry name" value="Subunit X (non-heme 7 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001361263.1"/>
        </source>
    </evidence>
    <sequence length="55" mass="6342" checksum="A4CB1D0E2C976756" modified="2020-08-12" version="1">MKVIYNTLFKRTSTYAVAIIGATFFFERAIDVSSTALFESINKGKLWKDIKSKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVB4</accession>
    <name>A0A6I8UVB4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4804993</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361465.3">
        <property type="nucleotide sequence ID" value="XM_001361428.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVB4"/>
    <dbReference type="FunCoup" id="A0A6I8UVB4">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804993"/>
    <dbReference type="InParanoid" id="A0A6I8UVB4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389:SF4">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361465.3"/>
        </source>
    </evidence>
    <sequence length="425" mass="48352" checksum="F537A1AB193B18E4" modified="2020-08-12" version="1">MRTKHLILILIGVIFATIVFIAVGPAGEPNDSFKNIVVQTHQQLKEFQENLRVGLERKKLEIDAKYLVLLGFTASKDGSPQQQQNELQQNHQQLPQTDLPAPATKAEQELSDLRRQHAQEAGANAQQTDDQEREKEMGQHRRITTPYNGTKSNFTIVTYVQEGQAPSAILLAQNIAVKLPGEHLLIYDLGVSEEQLKALNAYCNISRCAVITYDLSEFPSFVADQRTHAYRPIVIKDALLRARSVLFVENYMRMRGGYHELQELQSRGMVAGVLGWNTPTAVSSLTHPKMFDFFESDAEDFIFLRMVDLDVVFFVDNLFVTEKIMLPWLKCCLTQECIDPIGAQSNGCKFNKKPLYRYSGCHGYDASAFNIVLGLTWRWDNSKYSLPSDDAKNMFYKETLEEATRILESRRRNSSETSDHPFTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVF5</accession>
    <name>A0A6I8UVF5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein patched</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ptc</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361519.1">
        <property type="nucleotide sequence ID" value="XM_001361482.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UVF5">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="GeneID" id="4805055"/>
    <dbReference type="KEGG" id="dpo:4805055"/>
    <dbReference type="InParanoid" id="A0A6I8UVF5"/>
    <dbReference type="OMA" id="HLYDTEW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075384">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UVF5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097108">
        <property type="term" value="F:hedgehog family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008158">
        <property type="term" value="F:hedgehog receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005119">
        <property type="term" value="F:smoothened binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000027">
        <property type="entry name" value="Blast:Protein patched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000038">
        <property type="entry name" value="Blast:Protein patched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004766">
        <property type="entry name" value="TM_rcpt_patched"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00918">
        <property type="entry name" value="2A060602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46022">
        <property type="entry name" value="PROTEIN PATCHED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46022:SF1">
        <property type="entry name" value="PROTEIN PATCHED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
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        <ligand>
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        <ligand>
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        <ligand>
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        <ligand>
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    <feature type="binding site" evidence="6">
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        <ligand>
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        <ligand>
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    <feature type="binding site" evidence="6">
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        <ligand>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001361743.2"/>
        </source>
    </evidence>
    <sequence length="335" mass="37807" checksum="04C30C881BFF3B91" modified="2020-08-12" version="1">MQFGVLGSGIIGLTTALELQQQFPSAQVSIIADRFNEDTVSYVAAGIFRPGTSFMGPTQEITQQWMTDAFNYWDELRRSKEAPLAGVCQLSGYIYSRTSPSIVRNHFIEKLLPVYRRATDEELRLCQGGWKYGSFFTTCLTESRLFLPYATNKFLENGGQVVRQHVKNFFDVQQNFDVLVNCTGMGAKELCNDQHLVPIRGQVLKVRAPWIKTAFYGDYDTYVLPGFETVTLGGCRQFDSYNTEWCKYDSMAIKERCYDLLPSLKKAEIVRECVGLRPHRAIVRVEPELVTNAEGRRLKVVHNYGHGGYGVTTAPGTAKYAVKIVREMLAGNSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UX36</accession>
    <name>A0A6I8UX36_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 14">Alcohol dehydrogenase</fullName>
            <ecNumber evidence="6 14">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC6903474</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="6 14"/>
    <dbReference type="RefSeq" id="XP_002132353.2">
        <property type="nucleotide sequence ID" value="XM_002132317.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UX36"/>
    <dbReference type="KEGG" id="dpo:6903474"/>
    <dbReference type="InParanoid" id="A0A6I8UX36"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UX36">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05323">
        <property type="entry name" value="ADH_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000014">
        <property type="entry name" value="Mitochondrial-processing peptidase alpha subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002425">
        <property type="entry name" value="ADH_Drosophila-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF49">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01168">
        <property type="entry name" value="ALCDHDRGNASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01167">
        <property type="entry name" value="INSADHFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 14" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="15">
        <location>
            <begin position="97"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="16">
        <location>
            <begin position="251"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002132353.2"/>
        </source>
    </evidence>
    <sequence length="820" mass="89406" checksum="4EFAAFB16B44CAA1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXR8</accession>
    <name>A0A6I8UXR8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132917.2">
        <property type="nucleotide sequence ID" value="XM_002132881.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXR8"/>
    <dbReference type="KEGG" id="dpo:6903216"/>
    <dbReference type="InParanoid" id="A0A6I8UXR8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132917.2"/>
        </source>
    </evidence>
    <sequence length="153" mass="16698" checksum="12738B6D6AEC5630" modified="2020-08-12" version="1">MISGTDLIHKAIGVVGVLALLLAVSHSTARVSVHLGEGLSNMVESCARCLNSLTTVEGTPVLVDCENMGPTCSTIRRVGHRVYSAVERMVESGRSWTRAQPNPDDQQKTRPKVREKPASKKKTSSTLQENPNHKDSRWSSKQAGHTGENLRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V102</accession>
    <name>A0A6I8V102_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">X-linked retinitis pigmentosa GTPase regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6900881</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135238.3">
        <property type="nucleotide sequence ID" value="XM_002135202.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V102"/>
    <dbReference type="FunCoup" id="A0A6I8V102">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900881"/>
    <dbReference type="InParanoid" id="A0A6I8V102"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V102">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="49"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="192"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="459"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="842"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="888"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1029"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="733"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="481"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="573"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="608"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="626"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="936"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1048"/>
            <end position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002135238.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1071" mass="116619" checksum="8FB65A850D542E31" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1B9</accession>
    <name>A0A6I8V1B9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytosol aminopeptidase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135646.2">
        <property type="nucleotide sequence ID" value="XM_002135610.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1B9"/>
    <dbReference type="KEGG" id="dpo:6900325"/>
    <dbReference type="InParanoid" id="A0A6I8V1B9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V1B9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.220.10:FF:000005">
        <property type="entry name" value="cytosol aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M17 leucyl aminopeptidase N-terminal" evidence="1">
        <location>
            <begin position="55"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135646.2"/>
        </source>
    </evidence>
    <sequence length="225" mass="25180" checksum="8AC43E7AA3A3AA04" modified="2020-08-12" version="1">MMLGVVRRLVSQAFRQCLRPRPQAIQRRSVSQAVNQMLQLQQMDMCADPPSRGLVVSVFADETDRNDAGVLTATGWRYNVQKTAGRLLEVLRMSGPMPKRGEARLFFAIEPERIPYYSVVAVVGLGKECLGYNPYEVLDEQKEAIRRSVAAACRILAELDTDRIEVENCGHAESTAEGAALGIWLYQELRNPKNRIPVPSLDLYARKDEVCGLEGWRIGLQMAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1E3</accession>
    <name>A0A6I8V1E3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prominin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6900110</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135462.3">
        <property type="nucleotide sequence ID" value="XM_002135426.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1E3"/>
    <dbReference type="KEGG" id="dpo:6900110"/>
    <dbReference type="InParanoid" id="A0A6I8V1E3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V1E3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF1">
        <property type="entry name" value="PROMININ-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="508"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="554"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="871"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="973"/>
            <end position="996"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002135462.3"/>
        </source>
    </evidence>
    <sequence length="1015" mass="114365" checksum="4D4BA74ED3770477" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V1W6</accession>
    <name>A0A6I8V1W6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein Rpp14a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Rpp14a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002136931.1">
        <property type="nucleotide sequence ID" value="XM_002136895.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V1W6"/>
    <dbReference type="FunCoup" id="A0A6I8V1W6">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="GeneID" id="6896717"/>
    <dbReference type="KEGG" id="dpo:6896717"/>
    <dbReference type="InParanoid" id="A0A6I8V1W6"/>
    <dbReference type="OMA" id="CHFQVLK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248307">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002136931.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12580" checksum="2B80F6459BF47712" modified="2020-08-12" version="1">MKDSQYLDVKLKLRDTESIVLTPGYLIGCILNSLESIFGEIGGKTSLDIVKFSAAERRLILRVPQEFVERARIAVTLIGHYQEVPCHFQVLNASKNQLDFDSKNDENVVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2Y2</accession>
    <name>A0A6I8V2Y2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Telomere-binding protein cav</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cav</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137495.2">
        <property type="nucleotide sequence ID" value="XM_002137459.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2Y2"/>
    <dbReference type="FunCoup" id="A0A6I8V2Y2">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897342"/>
    <dbReference type="InParanoid" id="A0A6I8V2Y2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137495.2"/>
        </source>
    </evidence>
    <sequence length="283" mass="32099" checksum="743EE56D76419228" modified="2020-08-12" version="1">MPGTGLPEALLKYQAEEEQMVMSSAEEHEREFFRKYYPPCKIRPADLERVYSPEDVRELCLRTKVRVNMTHLNCVWDAKKRLNAKNRLENRSERFINAMLIKAVARKMVEHYTDEEVAEYNLVAKSELKKQNNFRLDRYKRERECIGPEATDVTTSFSQPLLNDSAAAVFVQPDQPTTPFNATTAIPENDAFLSTGIDSDDELWDIGQDEIEIPPETQLSESVSCDLGHDWVQAGLQVNSESMSLDDDISSQSQALPGHTVDSEDYNEFGTQVAAASTQENQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3A1</accession>
    <name>A0A6I8V3A1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Calmodulin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6897694</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137820.1">
        <property type="nucleotide sequence ID" value="XM_002137784.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3A1"/>
    <dbReference type="FunCoup" id="A0A6I8V3A1">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="GeneID" id="6897694"/>
    <dbReference type="KEGG" id="dpo:6897694"/>
    <dbReference type="InParanoid" id="A0A6I8V3A1"/>
    <dbReference type="OMA" id="RYANMST"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247695">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039508">
        <property type="entry name" value="KASH5_EF-hand-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14658">
        <property type="entry name" value="EF-hand_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="97"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="133"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002137820.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="18966" checksum="AEF945F43EECB949" modified="2020-08-12" version="1">MDEFDFSTPPPEERVSHSHTLTDEQSKDCTTVFDMFDPENTGVLPLKQIKYLMRALAVFPTDLELSDIYAEIDTDGSGDMLRDDFLYIMSKMYKDMTPEDEIIAAFKVFDKEGTGVIAEAEFRHIMTNLGDKMTEDEIEEIIRDADSDTEGNIDYVRFVTMMSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3K6</accession>
    <name>A0A6I8V3K6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">LOC6897738</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <strain evidence="12">MV2-25</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits VhaAC45 and ATP6AP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Vacuole membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137844.2">
        <property type="nucleotide sequence ID" value="XM_002137808.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038003.2">
        <property type="nucleotide sequence ID" value="XM_015182517.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3K6"/>
    <dbReference type="KEGG" id="dpo:6897738"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000001">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="11">
        <location>
            <begin position="24"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="11">
        <location>
            <begin position="100"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_002137844.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015038003.2"/>
        </source>
    </evidence>
    <sequence length="162" mass="16495" checksum="36632BAB58540553" modified="2020-08-12" version="1">MSSDDTSAVSEADRPPYALFFGGMGAASAIIFSALGAAYGTAKSGTGIAAMAVMRPELIMKSIIPVVMAGIIAIYGLVVSVLIAGSLSPTYTIRKGYIHLAAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAIYLYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V686</accession>
    <name>A0A6I8V686_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chitinase-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6898991</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138951.2">
        <property type="nucleotide sequence ID" value="XM_002138915.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V686"/>
    <dbReference type="KEGG" id="dpo:6898991"/>
    <dbReference type="InParanoid" id="A0A6I8V686"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026033037" evidence="5">
        <location>
            <begin position="21"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="7">
        <location>
            <begin position="23"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="389"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002138951.2"/>
        </source>
    </evidence>
    <sequence length="460" mass="53003" checksum="06E3CBDAA7347A3C" modified="2020-08-12" version="1">MCSLNCAFLLIIFMISGCAAKDFKIFCEYNLASFDYLDDSKFSSWEIDPRLCSYLVYANGVGVDGETGKVKITDRHGILENDLLSFASMTRKENIRRLLVTIGGWQEESSDFSKMAADSRKREQFISSVIDFIYEWRMDGIQIDWRYPTQRGGKPEDRKNFILLLKELKTMIDGHEFILMVAVLGRTDKSTLEYYDIPKLVQHVHYVNLMFYDNRDPYKMHLSYNAPLSGKNGVIESVKLWKKLSKAPGKLILNIPLFVRSYIMEKHKTTVGSPCKGPGLKTALTRKSGFMTYNEWCVQADTWTQKFDEQAQVPYAYRDDHWISFENNRSITEKLKLLKSQNLGGAISSTIDADDFSGKCGKKHALERVILETLDPQTVLVDMEAPTEPPPCLKDGVFRDINDCQLYYVCSAGHRSDYECRWQEFFDEAKSECRPMKEVKCEVLEAALKERELRARTRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V711</accession>
    <name>A0A6I8V711_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Facilitated trehalose transporter Tret1-2 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6897389</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_003736628.2">
        <property type="nucleotide sequence ID" value="XM_003736580.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V711"/>
    <dbReference type="FunCoup" id="A0A6I8V711">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V711"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V711">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF86">
        <property type="entry name" value="FACILITATED TREHALOSE TRANSPORTER TRET1-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="50"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_003736628.2"/>
        </source>
    </evidence>
    <sequence length="518" mass="56057" checksum="338E386F7A29505D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9G2</accession>
    <name>A0A6I8V9G2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Rtnl1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015036577.2">
        <property type="nucleotide sequence ID" value="XM_015181091.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9G2"/>
    <dbReference type="FunCoup" id="A0A6I8V9G2">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V9G2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V9G2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF2">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="505"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="387"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015036577.2"/>
        </source>
    </evidence>
    <sequence length="583" mass="62694" checksum="A615744624C13EB2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBF2</accession>
    <name>A0A6I8VBF2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26532410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015037772.1">
        <property type="nucleotide sequence ID" value="XM_015182286.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBF2"/>
    <dbReference type="GeneID" id="26532410"/>
    <dbReference type="KEGG" id="dpo:26532410"/>
    <dbReference type="InParanoid" id="A0A6I8VBF2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015037772.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="19398" checksum="6F53DC9E0DDA0068" modified="2020-08-12" version="1">MHPLKLALTLNAPSKHMRRTREAERGAVEYDWDMYSRGVSRTPGSRLLAKVMPLNCNSFRFVLRCLAIYLVLPIPEPSPFYPLDYGSHPPPSWVSVCLCLCLMQSSSVVPFNLRRVLDTNAAQKTTKKQKQKEPELKFYAETEAYIERDAEISRDGARRCTLHKGKKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VBL4</accession>
    <name>A0A6I8VBL4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Gamma-interferon-inducible lysosomal thiol reductase-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4802453</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015037814.1">
        <property type="nucleotide sequence ID" value="XM_015182328.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VBL4"/>
    <dbReference type="GeneID" id="4802453"/>
    <dbReference type="InParanoid" id="A0A6I8VBL4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081764">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VBL4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF68">
        <property type="entry name" value="GH19763P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015037814.1"/>
        </source>
    </evidence>
    <sequence length="221" mass="24826" checksum="3AC5DCF3AE842A62" modified="2020-08-12" version="1">MGGVRVNIILTVILIFFIWQGIRYHQSLLRKLCEQSLFQLKQLHITLLYESLCPDSRNFMHQLGPVHEELQQYMDIQLVPFGKSRSEQGGAIFHCQHGPAECEGNRVQSCVISSTANQSAQVKFVVCQMFALDSSRADQCASEAGLLTDVDHCADSETGTKLQLQAELVTKSYQPSFIPTIVYNGVFNQQLQDHSLRDFRGTVCHLLRQQNLLPSGSPVCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VC84</accession>
    <name>A0A6I8VC84_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Transient receptor potential channel pyrexia isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">wtrw</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015038223.1">
        <property type="nucleotide sequence ID" value="XM_015182737.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VC84"/>
    <dbReference type="FunCoup" id="A0A6I8VC84">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="GeneID" id="4802989"/>
    <dbReference type="InParanoid" id="A0A6I8VC84"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076163">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VC84">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000348">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000240">
        <property type="entry name" value="Water witch, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052076">
        <property type="entry name" value="TRP_cation_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47143:SF1">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47143">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL PROTEIN PAINLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="618"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="654"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="683"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="720"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="787"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="222"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="258"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="328"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="361"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="395"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="430"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="586"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="949"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015038223.1"/>
        </source>
    </evidence>
    <sequence length="998" mass="111670" checksum="A8CDB23FBB9BE989" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VCQ4</accession>
    <name>A0A6I8VCQ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription factor cwo isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cwo</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015038569.2">
        <property type="nucleotide sequence ID" value="XM_015183083.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VCQ4"/>
    <dbReference type="FunCoup" id="A0A6I8VCQ4">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VCQ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11440">
        <property type="entry name" value="bHLH-O_Cwo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000079">
        <property type="entry name" value="CLUMA_CG001539, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="74"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="8">
        <location>
            <begin position="140"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="276"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="353"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="459"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015038569.2"/>
        </source>
    </evidence>
    <sequence length="716" mass="77702" checksum="0AC5A5D85F8AA5AD" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VDZ2</accession>
    <name>A0A6I8VDZ2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Calcineurin subunit B type 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CanB2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Calcineurin is a calcium-binding and calmodulin-binding protein found in all cells from yeast to mammals, and a calcium-dependent, calmodulin-stimulated protein phosphatase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015039224.2">
        <property type="nucleotide sequence ID" value="XM_015183738.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VDZ2"/>
    <dbReference type="SMR" id="A0A6I8VDZ2"/>
    <dbReference type="FunCoup" id="A0A6I8VDZ2">
        <property type="interactions" value="1954"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26533502"/>
    <dbReference type="InParanoid" id="A0A6I8VDZ2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VDZ2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000047">
        <property type="entry name" value="Calcineurin subunit B type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="128"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015039224.2"/>
        </source>
    </evidence>
    <sequence length="170" mass="19267" checksum="FDB1BD9DB5A4BDEC" modified="2020-08-12" version="1">MGNETSLPMEMCSNFDADEIRRLGKRFRKLDLDNSGALSVDEFMSLPELQQNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVKGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIGFADKDEDGKISFDEFCSVVGNTDIHKKMVVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VJ01</accession>
    <name>A0A6I8VJ01_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial ribonuclease P catalytic subunit</fullName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Mitochondrial ribonuclease P protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">mldr</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="RefSeq" id="XP_015042353.2">
        <property type="nucleotide sequence ID" value="XM_015186867.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VJ01"/>
    <dbReference type="FunCoup" id="A0A6I8VJ01">
        <property type="interactions" value="1478"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VJ01"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030678">
        <property type="term" value="C:mitochondrial ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097745">
        <property type="term" value="P:mitochondrial tRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18718">
        <property type="entry name" value="PIN_PRORP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000006">
        <property type="entry name" value="AGAP001968-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033495">
        <property type="entry name" value="MRPP3_PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031595">
        <property type="entry name" value="PRORP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13547:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBONUCLEASE P CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16953">
        <property type="entry name" value="PRORP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PRORP" evidence="16">
        <location>
            <begin position="306"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_015042353.2"/>
        </source>
    </evidence>
    <sequence length="546" mass="62570" checksum="D573995467B02EBF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VN66</accession>
    <name>A0A6I8VN66_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">bel</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <strain evidence="14">MV2-25</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_015037549.1">
        <property type="nucleotide sequence ID" value="XM_015182063.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232467.1">
        <property type="nucleotide sequence ID" value="XM_033376576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VN66"/>
    <dbReference type="GeneID" id="6897432"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248670">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VN66">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18051">
        <property type="entry name" value="DEADc_DDX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000160">
        <property type="entry name" value="ATP-dependent RNA helicase DDX3X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="322"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="538"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="16"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="689"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="722"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="761"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015037549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033232467.1"/>
        </source>
    </evidence>
    <sequence length="803" mass="85648" checksum="F4896E9A51562BA0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNW8</accession>
    <name>A0A6I8VNW8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Uncharacterized protein SNF4Agamma isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">SNF4Agamma</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232754.1">
        <property type="nucleotide sequence ID" value="XM_033376863.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033232755.1">
        <property type="nucleotide sequence ID" value="XM_033376864.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000003">
        <property type="entry name" value="Protein kinase AMP-activated non-catalytic subunit gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF35">
        <property type="entry name" value="LD22662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="877"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="956"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="1031"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="1102"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="437"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1188"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="211"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="526"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="626"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1231"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1268"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1306"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1350"/>
            <end position="1365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033232754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033232755.1"/>
        </source>
    </evidence>
    <sequence length="1377" mass="149896" checksum="AE1E6533093DF855" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPW4</accession>
    <name>A0A6I8VPW4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Microtubule-associated protein Jupiter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Jupiter</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to all microtubule populations.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAP Jupiter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232789.1">
        <property type="nucleotide sequence ID" value="XM_033376898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VPW4"/>
    <dbReference type="FunCoup" id="A0A6I8VPW4">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899286"/>
    <dbReference type="InParanoid" id="A0A6I8VPW4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VPW4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033335">
        <property type="entry name" value="JUPITER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930">
        <property type="entry name" value="GEO05313P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930:SF2">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17054">
        <property type="entry name" value="JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="130"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033232789.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="39319" checksum="9A16E794CF2AD1F9" modified="2020-08-12" version="1">MAAYAAFKHVELYNVGKAKKRVLRPPGGGSSDIFGSDMPQTPRNVKNRMVSNIFSVEKDNSVKNTVRQGAHRFYFIGDNPRRGQKPVDSHSRLFGEPMRPITPGKNHMKSSIPFGQNTETAAAAQKLLTNGSSNANTTNGHQYNGKSGSVSSASSSVSSSTENLKMNSGSRSVYIRNMSRVEDNEKSKQIKTDTAGCPLTPVASAPAPPPADVLGIDLPCLDLEVGDVPKDNEIYTETGKHDVNIQTRRDSGSNVEQPHSLEKMRSSANLKEPLALCPDYVKEVHGPCNARNPITGLGLNGDGVGGLKPVKQKIREGNPVTGEGYRAGGTDYIKAAGSTNSGSVGNGDNGGNSVVNKNRVPPGGYSSGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQ48</accession>
    <name>A0A6I8VQ48_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein CheA84a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CheA84a</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233167.1">
        <property type="nucleotide sequence ID" value="XM_033377276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQ48"/>
    <dbReference type="FunCoup" id="A0A6I8VQ48">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897267"/>
    <dbReference type="InParanoid" id="A0A6I8VQ48"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21112">
        <property type="entry name" value="CHEMOSENSORY PROTEIN A 29A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21112:SF0">
        <property type="entry name" value="CHEMOSENSORY PROTEIN A 29A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033233167.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29713" checksum="CD44E9816EA64165" modified="2020-08-12" version="1">MAASIRTETETKTKTETQQQFPLPATPSAEEKEEDDKSISSVPFVEIPIESEFTKDQEQEQEQEQWREQDLYHHLVYGVQVRHEARALARALQCILQTHLIPKPYEARLVSFISLSDGVWDVSKVRLIGRDRKMNGTISLLEDMDDDQYSFYAEAYIDTTGSGIYKKLPYTMPMTPACKGFKMYLPYFADSAKVGVNIDFPVDGPPCPLPKGTYYLKDILLNTSKWPSVMPRGYMKGVSYFFKNGKSAGVLEVTTHVVDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQU5</accession>
    <name>A0A6I8VQU5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">CSC1-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Tmem63</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033233450.1">
        <property type="nucleotide sequence ID" value="XM_033377559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQU5"/>
    <dbReference type="FunCoup" id="A0A6I8VQU5">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804346"/>
    <dbReference type="InParanoid" id="A0A6I8VQU5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070900">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="594"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="650"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="684"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="121"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="245"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="409"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033233450.1"/>
        </source>
    </evidence>
    <sequence length="770" mass="87303" checksum="7443DC88A3315528" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VVI3</accession>
    <name>A0A6I8VVI3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MD-2-related lipid-recognition domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4804225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234594.1">
        <property type="nucleotide sequence ID" value="XM_033378703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VVI3"/>
    <dbReference type="KEGG" id="dpo:4804225"/>
    <dbReference type="InParanoid" id="A0A6I8VVI3"/>
    <dbReference type="OMA" id="TSAPFVM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF1">
        <property type="entry name" value="MD-2-RELATED LIPID-RECOGNITION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033234594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17448" checksum="A1406C46ABFE664D" modified="2020-08-12" version="1">MNFIMESLETFCDHDYVEYFRKVPDAGLLYTFRVARPAAAFSIDITGKVLKTQKVMYRTENINGCEFLRNPLMSKVFGIVYKHLVVNGTFFECPIKPNVYFLKNEGAMAVLPSFHPPGRFQLSMRVKMAESRQPFVMEMLWKYRIVRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VW75</accession>
    <name>A0A6I8VW75_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pre-mRNA-splicing factor CWC22 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ncm</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235331.1">
        <property type="nucleotide sequence ID" value="XM_033379440.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VW75"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VW75">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000004">
        <property type="entry name" value="pre-mRNA-splicing factor CWC22 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="7">
        <location>
            <begin position="756"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="707"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="989"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="80"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="329"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="709"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="723"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="989"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1031"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1048"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1066"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1103"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1150"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1198"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1353"/>
            <end position="1363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033235331.1"/>
        </source>
    </evidence>
    <sequence length="1363" mass="152586" checksum="E9C4159E52A58BE8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W037</accession>
    <name>A0A6I8W037_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NF-kappa-B inhibitor alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">I-kappa-B-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC26532810</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NF-kappa-B inhibitor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236618.1">
        <property type="nucleotide sequence ID" value="XM_033380727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W037"/>
    <dbReference type="FunCoup" id="A0A6I8W037">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532810"/>
    <dbReference type="InParanoid" id="A0A6I8W037"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246788">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071356">
        <property type="term" value="P:cellular response to tumor necrosis factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051070">
        <property type="entry name" value="NF-kappa-B_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680:SF1">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033236618.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="31300" checksum="EE0021AD8145AA32" modified="2020-08-12" version="1">MAMEFQKARILSLQGLLPIHDDILDGDIYGVRRQIFVCKHSNFDINEMISADGEDCLELALTNDCDPDLVTALLTAGCVPNHIYENGNTALHIAVINNIDRESIRQLMLRIDLDLLLQTNDAGYTALHLAVRHNQYHVAETILDCIDERQLEGGAVYRRATETTDSKLTPEKAFAKYYERACDRLETTKDVLKNRRLKLDILNTAELMAGNTPLFFAVEQGQEHICYFLLAHLCDPDQENLSGHSPKSFHYEYARILRINLKISRVMDKVIGILNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0G5</accession>
    <name>A0A6I8W0G5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4818028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236244.1">
        <property type="nucleotide sequence ID" value="XM_033380353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0G5"/>
    <dbReference type="KEGG" id="dpo:4818028"/>
    <dbReference type="InParanoid" id="A0A6I8W0G5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W0G5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031958">
        <property type="entry name" value="DUF4778"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16008">
        <property type="entry name" value="DUF4778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="755"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="813"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="576"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="814"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236244.1"/>
        </source>
    </evidence>
    <sequence length="832" mass="91148" checksum="A2B194AB6D17657D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W191</accession>
    <name>A0A6I8W191_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">LOC26532134</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2 3">MV-25-SWS-2005</strain>
            <tissue evidence="2 3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237115.1">
        <property type="nucleotide sequence ID" value="XM_033381224.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237116.1">
        <property type="nucleotide sequence ID" value="XM_033381225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W191"/>
    <dbReference type="KEGG" id="dpo:26532134"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033237115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033237116.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="23513" checksum="1FA24562AC9DC9CC" modified="2020-08-12" version="1">MAEVVLRSAFWLSFIKMMEDHKQLSCYMLRQSLCSTQIQSIQMIFKEFFPCFQKGGSHNVYILPGIEPSRESYLSAILNFPMAEVVLRSAFWLSFIKMMEDHKQLSCYMLRQSLCSTQIQSIQMIFKEFFPCFQKGGSHNVFILPGIEPSRESYLSAILNFPMAEVVLRSAFWLSFIKMMKDHKQLSCYMLRQSLCSTRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W382</accession>
    <name>A0A6I8W382_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Guanylate cyclase</fullName>
            <ecNumber evidence="3 15">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21 22 23 24 25 26 27" type="primary">LOC6900273</name>
    </gene>
    <organism evidence="20 27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22 23" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="21 22 23">MV-25-SWS-2005</strain>
            <tissue evidence="21 22 23">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 15"/>
    <dbReference type="RefSeq" id="XP_015044051.2">
        <property type="nucleotide sequence ID" value="XM_015188565.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237790.1">
        <property type="nucleotide sequence ID" value="XM_033381899.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237791.1">
        <property type="nucleotide sequence ID" value="XM_033381900.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237792.1">
        <property type="nucleotide sequence ID" value="XM_033381901.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237793.1">
        <property type="nucleotide sequence ID" value="XM_033381902.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237794.1">
        <property type="nucleotide sequence ID" value="XM_033381903.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237795.1">
        <property type="nucleotide sequence ID" value="XM_033381904.1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900273"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W382">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Nucleotide cyclase"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <location>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_033237792.1"/>
        </source>
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        <source>
            <dbReference type="RefSeq" id="XP_033237793.1"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033237794.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033237795.1"/>
        </source>
    </evidence>
    <sequence length="1534" mass="170971" checksum="82D2E1D8FE5B74DC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4G8</accession>
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        <submittedName>
            <fullName evidence="10">Clusterin-associated protein 1</fullName>
        </submittedName>
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        <name evidence="10" type="primary">Cluap1</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
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        <subcellularLocation>
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            <location evidence="1">Cilium</location>
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        <text evidence="2">Belongs to the CLUAP1 family.</text>
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        <property type="entry name" value="CLUSTERIN ASSOCIATED PROTEIN 1"/>
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    <dbReference type="Pfam" id="PF10234">
        <property type="entry name" value="Cluap1"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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            <end position="338"/>
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    <feature type="region of interest" description="Disordered" evidence="8">
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    </feature>
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    <feature type="compositionally biased region" description="Gly residues" evidence="8">
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        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008340"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033237589.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="56536" checksum="74098CE91253BF44" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4S1</accession>
    <name>A0A6I8W4S1_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Formin-J isoform X1</fullName>
        </submittedName>
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        <name evidence="10" type="primary">simj</name>
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    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
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        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033238287.1">
        <property type="nucleotide sequence ID" value="XM_033382396.1"/>
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        <property type="interactions" value="509"/>
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    <dbReference type="KEGG" id="dpo:4812933"/>
    <dbReference type="InParanoid" id="A0A6I8W4S1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W4S1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040386">
        <property type="entry name" value="P66"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032346">
        <property type="entry name" value="P66_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455:SF7">
        <property type="entry name" value="SIMJANG, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR P66-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16563">
        <property type="entry name" value="P66_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional repressor p66 coiled-coil MBD2-interaction" evidence="8">
        <location>
            <begin position="210"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="334"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="615"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="663"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="907"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="208"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="58"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="385"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="407"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="615"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="672"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="698"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="746"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="907"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="947"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033238287.1"/>
        </source>
    </evidence>
    <sequence length="963" mass="101156" checksum="7FF48594E56EEF08" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5U5</accession>
    <name>A0A6I8W5U5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Septin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Septin1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238731.1">
        <property type="nucleotide sequence ID" value="XM_033382840.1"/>
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        <property type="interactions" value="1525"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000064">
        <property type="entry name" value="Septin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
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    </dbReference>
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        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="11">
        <location>
            <begin position="49"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="8">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR006698-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033238731.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="43557" checksum="2F933F15B486B9A1" modified="2020-08-12" version="1">MWVFRRRSNDTARRSPALTSFLQFSSIETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLVNSLFLTDLYPERIIPDAIEKQKQTVKLEASTVEIEERGVKLRLTVVDTPGFGDAIDNSNSFSAILEYIDEQYERFLRDESGLNRRNIVDNRIHCCFYFISPFGHGLKPLDVEFMKKLHSKVNIVPVIAKADCLTKKEILRLKCRIMQEIESHGIKIYPLPDCDSDEDEDYKEQVKQLKEAVPFAVCGANTLLEVKGKKVRGRLYPWGVVEVENPDHCDFIKLRTMLITHMQDLQEVTQEVHYENYRSDRLAKGIKGKENGIKQERENNVNAQTVANSALSEKDRILQEKEAELARMTEMLAQMQARMQAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W650</accession>
    <name>A0A6I8W650_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UNC93-like protein MFSD11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Major facilitator superfamily domain-containing protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC6896926</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <strain evidence="10 11">MV2-25</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137111.1">
        <property type="nucleotide sequence ID" value="XM_002137075.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238154.1">
        <property type="nucleotide sequence ID" value="XM_033382263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W650"/>
    <dbReference type="GeneID" id="6896926"/>
    <dbReference type="KEGG" id="dpo:6896926"/>
    <dbReference type="OMA" id="QFQDKTH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248402">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17407">
        <property type="entry name" value="MFS_MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF0">
        <property type="entry name" value="UNC93-LIKE PROTEIN MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041910"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002137111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033238154.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="47881" checksum="AD847CA43B9E8258" modified="2020-08-12" version="1">MDRKFLMILVLGCGFMFVFTAFQTMCNIEKTILDSISQEDDTFKGEGYTSLAIIYLFFSLSNWLAPSFISFTGPRVAMVVGALTYTAFMITFMFPSTVLLYVGSAVLGLGASITWTGQGTYLARCSESTTISRNSGVFWALLQCSMFIGNLFVYYQFQDKTRIDKETRNLVIGVLTVIAVLGIVFLAALRSMPDNAEHDNELEQKHTGWDQAMYALKSAGQLFFTKKMLLLSLAFFYTGLELSFFSGVFGSAIGFTTKIAETPKEIVGLVGICIGAGEVFGGGLFGILGNKTTRFGRDPIVIVGYIMHMAAFFMTFINLPNSAPFKDTTDVSYLETPSASIALVCAFLLGLGDACFNTQIYSMLGGEYVNNAVGAFALFKFTQSVAAAISFFYSSHFGLYVQLAILVVTGTIGTIAFVFVEWGFKRQHREQEALDK</sequence>
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<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6A8</accession>
    <accession>A0A0R3P0V2</accession>
    <name>A0A6I8W6A8_DROPS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein, isoform B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117184590</name>
        <name evidence="2" type="synonym">Dpse\GA32812</name>
        <name evidence="5" type="synonym">LOC117184714</name>
        <name evidence="2" type="ORF">Dpse_GA32812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
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            </authorList>
            <dbReference type="PubMed" id="15632085"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
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                <consortium name="Drosophila 12 Genomes Consortium"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
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                <person name="Richards S."/>
                <person name="Han Y."/>
                <person name="Wang M."/>
                <person name="Vee V."/>
                <person name="Qu J."/>
                <person name="Qin X."/>
                <person name="Muzny D.M."/>
                <person name="Reid J.G."/>
                <person name="Worley K.C."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="23185243"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2015-11" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="FlyBase"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4 5">MV-25-SWS-2005</strain>
            <tissue evidence="4 5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="CH475782">
        <property type="protein sequence ID" value="KRT05295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238832.1">
        <property type="nucleotide sequence ID" value="XM_033382941.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239297.1">
        <property type="nucleotide sequence ID" value="XM_033383406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W6A8"/>
    <dbReference type="KEGG" id="dpo:117184590"/>
    <dbReference type="KEGG" id="dpo:117184714"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0273219">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="101"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KRT05295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033238832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239297.1"/>
        </source>
    </evidence>
    <sequence length="139" mass="15405" checksum="B9F463C2E5508EBE" modified="2020-08-12" version="1">MAERGQYPQVQRRPNATEALWEGAKQEAVDRELIPSIPKAKVLFPIALQGERALKLLQRQNPGVLESEEKPLMKASCYMAHDRPAPQDELGSLVTEAGPKNHQLIISTDSNAHQNVWGSPDIKDNGQADISQEEESKQA</sequence>
</entry>
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    <accession>A0A6I8W6D3</accession>
    <name>A0A6I8W6D3_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184614</name>
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    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238916.1">
        <property type="nucleotide sequence ID" value="XM_033383025.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8W6D3"/>
    <dbReference type="KEGG" id="dpo:117184614"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="baseline"/>
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    </dbReference>
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        <property type="entry name" value="ADGR_G7"/>
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    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767:SF2">
        <property type="entry name" value="GPS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="798"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="971"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238916.1"/>
        </source>
    </evidence>
    <sequence length="1040" mass="120668" checksum="8DE1A8A51CC7C8B3" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8V1</accession>
    <name>A0A6I8W8V1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytokine receptor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">et</name>
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    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239796.1">
        <property type="nucleotide sequence ID" value="XM_033383905.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A6I8W8V1"/>
    <dbReference type="FunCoup" id="A0A6I8W8V1">
        <property type="interactions" value="3"/>
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    <dbReference type="KEGG" id="dpo:4814595"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W8V1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="800"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239796.1"/>
        </source>
    </evidence>
    <sequence length="840" mass="95061" checksum="24ABAF27BEAD0790" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W917</accession>
    <accession>Q29HA8</accession>
    <name>A0A6I8W917_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein elav</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Embryonic lethal abnormal visual protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">elav</name>
        <name evidence="11" type="synonym">LOC4814760</name>
    </gene>
    <organism evidence="10 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12 13">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="11 12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM elav family.</text>
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    <dbReference type="RefSeq" id="XP_001354795.3">
        <property type="nucleotide sequence ID" value="XM_001354759.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239771.1">
        <property type="nucleotide sequence ID" value="XM_033383880.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239772.1">
        <property type="nucleotide sequence ID" value="XM_033383881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W917"/>
    <dbReference type="SMR" id="A0A6I8W917"/>
    <dbReference type="GeneID" id="4814760"/>
    <dbReference type="KEGG" id="dpo:4814760"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078073">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050686">
        <property type="term" value="P:negative regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12650">
        <property type="entry name" value="RRM1_Hu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12652">
        <property type="entry name" value="RRM2_Hu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12377">
        <property type="entry name" value="RRM3_Hu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000480">
        <property type="entry name" value="Fne, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000510">
        <property type="entry name" value="protein elav isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000205">
        <property type="entry name" value="Sex lethal, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006548">
        <property type="entry name" value="ELAD_HU_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034775">
        <property type="entry name" value="Elav_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01661">
        <property type="entry name" value="ELAV_HUD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="166"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="265"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="419"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="135"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="78"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001354795.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033239771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033239772.1"/>
        </source>
    </evidence>
    <sequence length="500" mass="53120" checksum="0E480937EBB8DD62" modified="2020-08-12" version="1">MDFMMANTGAAGGVDTQAQLMQSAAAAAAVAATSAATAPVQNAAAVAAAAQLQQQQQVQQAILQVQQQQTQQAVAAAAAAVTQQLQQQQQAVAQQQQAQQQQAQQVQQQVQQQQAQQAQVQAVVAQQQVQQQVQQTVVPQPQPPPNTNGNAGVPQNGSNGSTETRTNLIVNYLPQTMTEDEIRSLFSSVGEIESVKLIRDKSQVYIDPLNPQAPSKGQSLGYGFVNYVRPQDAEQAVNVLNGLRLQNKTIKVSFARPSSDAIKGANLYVSGLPKTMTQQELEAIFAPFGAIITSRILQNAGNDTQTKGVGFIRFDKREEATRAIIALNGTTPSSCTDPIVVKFSNTPGSTSKIIQPQLPAFLNPQLVRRIGGAMHTPVNKGLARFSPMAGDMLDVMLPNGLGAAAAAATTLASGPGGAYPIFIYNLAPETEEAALWQLFGPFGAVQSVKIVKDPTTNQCKGYGFVSMTNYDEAAMAIRALNGYTMGNRVLQVSFKTNKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W9H6</accession>
    <name>A0A6I8W9H6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC117184902</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239932.1">
        <property type="nucleotide sequence ID" value="XM_033384041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W9H6"/>
    <dbReference type="KEGG" id="dpo:117184902"/>
    <dbReference type="InParanoid" id="A0A6I8W9H6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033239932.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="40417" checksum="257A501B8BF40249" modified="2020-08-12" version="1">MAARLNEIPVELRGQGPPAAETCENEREDEAAADQDSTKSERKEKNEKAPQAPGHNNNHGEYRAEVEMLKLQIELLKLQSEREGRREGRGENTTPAANNDAGVMLLNAAKDMLPTYHGNISGNNDDVTTWIAQFKAVAKVNKLKDEKLLMLLMSKLKDKALVWLHSSPEHMSLPIDQLLNVMEDTFHPKESKLLLRRKFESRSWARGEEFSMYFNAKVSLASRIVIDDEEFIDGVIEGIPDVGLRRQAHMQCFGAPYQLLKAFEKIMLPKKYGSTEGANTGASPTPIRCYNCNSLGHVAGECRKPKRERGACYGCGSMSHRDTWCHCVTVTKRSTRLPHRLREPNKFYEVIVSRAPVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBT7</accession>
    <name>A0A6I8WBT7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240853.1">
        <property type="nucleotide sequence ID" value="XM_033384962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WBT7"/>
    <dbReference type="KEGG" id="dpo:26532322"/>
    <dbReference type="InParanoid" id="A0A6I8WBT7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="1">
        <location>
            <begin position="64"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240853.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="27530" checksum="F05649A0A3F40894" modified="2020-08-12" version="1">MDDRDIAIISAVVAQQNIFLHQLIMLLNDDDDEDFNFDEDEMLDWVTAIKTQKPGRLWSYKRFGTFWEKDVPENNEAFFKESFRMERPCFDILVNRLEVLRKKDTNCRAAIPLEKRIAIALYTLGSSSEYRTVGRLFGVAPNSVCNILHEFCRALIHEFSNEYMSPNYLTSDKIDECVKGFEAIGFPQCLGAIDACHIEIKPPAAEAVDHHNSKGWYSTVLFALVDHRYANANILHKNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WC24</accession>
    <name>A0A6I8WC24_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glucose dehydrogenase [FAD, quinone]</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6902142</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240109.1">
        <property type="nucleotide sequence ID" value="XM_033384218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WC24"/>
    <dbReference type="FunCoup" id="A0A6I8WC24">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902142"/>
    <dbReference type="InParanoid" id="A0A6I8WC24"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF226">
        <property type="entry name" value="RE28171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="4">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="610"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="296"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="912"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="212"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="921"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033240109.1"/>
        </source>
    </evidence>
    <sequence length="933" mass="102410" checksum="EA9253C0CEDD842E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WD12</accession>
    <name>A0A6I8WD12_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4802152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241283.1">
        <property type="nucleotide sequence ID" value="XM_033385392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WD12"/>
    <dbReference type="KEGG" id="dpo:4802152"/>
    <dbReference type="InParanoid" id="A0A6I8WD12"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006631">
        <property type="entry name" value="DM4_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398">
        <property type="entry name" value="AGAP007094-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21398:SF1">
        <property type="entry name" value="FI03705P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07841">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00718">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033241283.1"/>
        </source>
    </evidence>
    <sequence length="461" mass="51653" checksum="AD0CEF6CBF6F3E45" modified="2020-08-12" version="1">MAKTIRLIGCAAGVTAMLILLLPLCLLRNDRPKANGDGNSNRSHQAVSPTPAPTPAPTPATLTTSTDGAPFNDSWRHHMLRLHRQKRYLLFPEGSSFQLVFDIIIPIVDYTNYAILGITCAVAWELPSKAPSELIENVLTRINDGTIGTVRRNGSAPDPEKDPNRSPANWQPVHAPSAVRTSQPRTPDRQPAHPIAANSHGSYYTNILRGSGSAGTSAYANANWNANANGQQQLQATLPGTAPGPANWHARQSVAKWQRPRTGDPPPPPDNWWRRNKDRLQQNWREKQAMWSPSAGQSKWDIDYSQRPRRLLKSPPQHHIYPVFGKRRRRRRRSLEQDPLFERLHLQQHLSSRQLLFGKIERLYKAQQLNGTACVLRALCESAQRQQQSDGGEGGGAKAFRPQSFIMELLSAIFQLPASNDGDEVEALALKHISPHYLEAHRQQGNCPQLYSDCRHAFWFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WE36</accession>
    <name>A0A6I8WE36_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox protein ceh-24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">scro</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable transcriptional regulator that is required in neural development for the normal formation of sublateral cholinergic motor neuron processes. Plays a role in regulating the expression of acetylcholine transporter protein unc-17 in the sublateral processes. In particular, it is required in sublateral motor neurons for a left-right turning behavior that occurs during the lethargus phase of the normal sleep process called 'flipping'. During 'flipping' animals rotate 180 degrees about their longitudinal axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NK-2 homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241653.1">
        <property type="nucleotide sequence ID" value="XM_033385762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WE36"/>
    <dbReference type="FunCoup" id="A0A6I8WE36">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117185621"/>
    <dbReference type="InParanoid" id="A0A6I8WE36"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000296">
        <property type="entry name" value="Scarecrow, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF41">
        <property type="entry name" value="MUSCLE-SPECIFIC HOMEOBOX PROTEIN TINMAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="250"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="252"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="311"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033241653.1"/>
        </source>
    </evidence>
    <sequence length="467" mass="50458" checksum="A4FA9D841CDACB60" modified="2020-08-12" version="1">MSASNLIDISINEKKCYREISINGEPNLTALNESDLVMSLSPKDILIPLAISQHHQLEPPNKLNTNETSAQNPISTAAAVAVAHHHQNISNIHHLQSLHSQHQSSLFNNNHSTPFSVTDILSPIEESYRKLELNGNPPSPFRSNSSGSSINSPGTLSASTMANPYAMGSLYHSPGVQSYCGPTDNLSLAGHYTDMRSSASWYGSTANDPRFAISRLMSTTASGSMSHMGNMSGLAACSVSDTKPLQFPLAQRRKRRVLFTQAQVYELERRFKQQRYLSAPEREHLASLIHLTPTQVKIWFQNHRYKCKRQAKEKAMAEQNQHNQSASSPRRVAVPVLVKDGKPCSGSNNTTQSQTHGNSSTPSGNNSSASNSGNANSGAVAVSSNVTSGLNLISGDAPNSHSPDTSSSLLASYGTVGGSNVAMLQQPCNNTLMSNSLAMAYRNQNNFISNGHQQQCGGYLPLQGRAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>B5DJJ0</accession>
    <accession>A0A6I8UXY5</accession>
    <name>B5DJJ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133060.1">
        <property type="nucleotide sequence ID" value="XM_002133024.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DJJ0"/>
    <dbReference type="GeneID" id="6902518"/>
    <dbReference type="KEGG" id="dpo:6902518"/>
    <dbReference type="eggNOG" id="ENOG502T8G4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1278810_0_0_1"/>
    <dbReference type="InParanoid" id="B5DJJ0"/>
    <dbReference type="OMA" id="QDYKQQR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250334">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043758195" evidence="2">
        <location>
            <begin position="17"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133060.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="22898" checksum="B652457F4426B705" modified="2008-10-14" version="1">MIIVLLLLFQSLCLYCQRLVLHIHDRCFPRNVRLLQEVAETVGDRKAQQHLEQQELEQTKRRQKRRILEPILPLGAGVNSEACRFCAHSPQGYCRHHFHLQELQLHQQRNSSRIGGDQDYKQLRRIKRELKRSLGQRQPQLRSYRPLLSGSPSCSSLSSGYGSISTCSTYADEEEEAEEEEQHDESVEVPGNLVTTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>B5DJN5</accession>
    <accession>A0A6I8UY64</accession>
    <name>B5DJN5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine-rich cell wall structural protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6902577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133105.1">
        <property type="nucleotide sequence ID" value="XM_002133069.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DJN5"/>
    <dbReference type="STRING" id="46245.B5DJN5"/>
    <dbReference type="GeneID" id="6902577"/>
    <dbReference type="KEGG" id="dpo:6902577"/>
    <dbReference type="eggNOG" id="ENOG502RTNX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_657690_0_0_1"/>
    <dbReference type="InParanoid" id="B5DJN5"/>
    <dbReference type="OMA" id="GHGCKFG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250203">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044264942" evidence="2">
        <location>
            <begin position="17"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002133105.1"/>
        </source>
    </evidence>
    <sequence length="400" mass="31915" checksum="E149D53BAC56726F" modified="2008-10-14" version="1">MRLFIVLSVCVALASAGYGGGYGGGGGGGGGGGHASSSASASASAGSIAGGDGGGKFGGGPGPGLGSGGGFDGGLSGPGFGGGGQGGPFGGGAQHGGGETGGGGFGGSGASSGAHGSSGGEGLIGGGGAHGSGGAHGAGGGFIGGGGGQGLGGGLGGSGGHGSGGAGGGSIGGGFGAGGSSGSASAGSGGGLFGGHGSGKPGGGFGGGNFGGDLGGSGGFNGGGGGHGLGGGLGGSGGHGSGGAGGGSSGGGFGAGGSSGSASAGSGGGLLGGHGSGKPGGGYGGGHFGGDIGFGGGGIGIGHGPGIGGGHSHGAGLGGPFAGGFTGGNGVGKGGGYGGGGGGGYGGGGGGGYGGGGHGGHGGLGGAGGKHGGYGGGAHSSASSAASATASAGSGGGYHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>B5DKH1</accession>
    <accession>A0A6I8UYJ6</accession>
    <name>B5DKH1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RING-box protein 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Roc1a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133392.2">
        <property type="nucleotide sequence ID" value="XM_002133356.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DKH1"/>
    <dbReference type="FunCoup" id="B5DKH1">
        <property type="interactions" value="1652"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DKH1"/>
    <dbReference type="GeneID" id="6901854"/>
    <dbReference type="KEGG" id="dpo:6901854"/>
    <dbReference type="eggNOG" id="KOG2930">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115512_2_1_1"/>
    <dbReference type="InParanoid" id="B5DKH1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244243">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DKH1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16485">
        <property type="entry name" value="mRING-H2-C3H2C2D_RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000010">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002133392.2"/>
        </source>
    </evidence>
    <sequence length="112" mass="13139" checksum="19A967AD9A096309" modified="2016-01-20" version="2">MDRNMEVDEEEFEVPSSSSKGEKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWDFQKYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPS8</accession>
    <accession>A0A6I8V0S6</accession>
    <name>B5DPS8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">COMM domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900632</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135005.2">
        <property type="nucleotide sequence ID" value="XM_002134969.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPS8"/>
    <dbReference type="FunCoup" id="B5DPS8">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DPS8"/>
    <dbReference type="GeneID" id="6900632"/>
    <dbReference type="KEGG" id="dpo:6900632"/>
    <dbReference type="eggNOG" id="ENOG502QSP3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095496_0_0_1"/>
    <dbReference type="InParanoid" id="B5DPS8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245203">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DPS8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047155">
        <property type="entry name" value="COMMD4/6/7/8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231:SF4">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4-8 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002135005.2"/>
        </source>
    </evidence>
    <sequence length="194" mass="21486" checksum="D8C6DBFE45D561B4" modified="2016-01-20" version="2">MKFRFCGEGDCPDWVLAEIISTLSNLSIENLEKLSDLVAQRICGGAFEEAAIKSLTSTLTNEGKTAVACINFMLINASRYNCTESIFGEEIQQLGLPKDHAAAMCRVLQKHSGSIRQKLLDKAFRINELQSVRNVTSPGETPANCATLELKISQELVDGLPKDTTHIVNFERSHLKALLAELKVARDIMDKYEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-11-25" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q298N4</accession>
    <name>ZFPL1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Zinc finger protein-like 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA18838</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ZFPL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL27921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q298N4"/>
    <dbReference type="FunCoup" id="Q298N4">
        <property type="interactions" value="1345"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q298N4"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0283584">
        <property type="protein sequence ID" value="FBpp0282022"/>
        <property type="gene ID" value="FBgn0078838"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801735"/>
    <dbReference type="eggNOG" id="KOG3970">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075387_0_0_1"/>
    <dbReference type="InParanoid" id="Q298N4"/>
    <dbReference type="OMA" id="HDHDYNP"/>
    <dbReference type="PhylomeDB" id="Q298N4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078838">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16487">
        <property type="entry name" value="mRING-H2-C3DHC3_ZFPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058730">
        <property type="entry name" value="U-box_ZFPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039043">
        <property type="entry name" value="ZFPL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058731">
        <property type="entry name" value="Znf-B_box_ZFPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12981">
        <property type="entry name" value="ZINC FINGER PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12981:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25998">
        <property type="entry name" value="U-box_ZFPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25993">
        <property type="entry name" value="zf-B_box_ZFPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000355180" description="Zinc finger protein-like 1 homolog">
        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="B box-type; degenerate">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-type; atypical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="168"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="302" mass="33772" checksum="82659B7EA5FC1377" modified="2006-04-04" version="1">MGLCKCPKRLVTNQFCFEHRVNVCEHCMVQSHPKCIVQSYLQWLRDSDYISNCNLCGTSLEQGECVRLVCYHVFHWDCLNARQAALPANTAPRGHQCPGCSVEIFPNTNLVSPVADALKNYLAQVNWGRNGLGLALLSEDQSSSLKAIKSKASVSQAAVSNMTKVHHIHSGGERERGKPNGGDASTPHSVLLMDAFNPPSSGDFNASSRRPLLPRQSPIGGTDRDDNKYQRRTPAALLSRWTRRFYSPSSRPPWRRTWFLVLSGILAFVMFIYLLAWMGRSGSNDGLDESWNNPNPQPNHYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q299D3</accession>
    <name>PURA_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenylosuccinate synthetase</fullName>
            <shortName evidence="2">AMPSase</shortName>
            <shortName evidence="2">AdSS</shortName>
            <ecNumber evidence="2">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GA14431</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="2"/>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL27770.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q299D3"/>
    <dbReference type="SMR" id="Q299D3"/>
    <dbReference type="FunCoup" id="Q299D3">
        <property type="interactions" value="1522"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q299D3"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0283686">
        <property type="protein sequence ID" value="FBpp0282124"/>
        <property type="gene ID" value="FBgn0074459"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801557"/>
    <dbReference type="eggNOG" id="KOG1355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_3_0_1"/>
    <dbReference type="InParanoid" id="Q299D3"/>
    <dbReference type="OMA" id="QSYVRFL"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074459">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000002">
        <property type="entry name" value="Adenylosuccinate synthetase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000399263" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="36"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="321"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="353"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="436"/>
            <end position="438"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03125"/>
        </source>
    </evidence>
    <sequence length="448" mass="49281" checksum="B79B658E8B4D2A6C" modified="2008-10-14" version="2">MSTTNAINGNLYEQLHQGRTNMYKSKVDVVLGAQWGDEGKGKVVDMLASEVDIVCRCQGGNNAGHTVVANGTEFDFHLLPSGVVNEKCISVIGNGVVIHLPSLFDEVLKNEAKGLQHLEHRLIISDRAHLVFDFHQHVDGMQEAEKGGKSLGTTKKGIGPAYSSKATRNGIRVGELLGDFNAFSDKFKLIVATHLRLFPSINVDVEAELTRYRDYAEKVRPYVKDTICFLHTALRNGKTILVEGANAAMLDIDFGTYPYVTSSNCSIGGVLTGLGLPPQTIGEVIGVVKAYTTRVGDGPFPSEQLNEIGDLLQTRGFEVGVTTKRKRRCGWLDIPLLRYTSLVNGYTCICLTKLDILDTLPEIKVAVSYKKANGDKLDHFPGTIAELGNIEVEYAVLPGWQTSTEHIRNFKELPENAQNYVRFLEKELSVPVRWVGVGKGRESIINVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D10</accession>
    <accession>A0A6I8VM45</accession>
    <name>Q29D10_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">Pex7</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13 14">MV-25-SWS-2005</strain>
            <tissue evidence="13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat peroxin-7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353103.2">
        <property type="nucleotide sequence ID" value="XM_001353067.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015044149.1">
        <property type="nucleotide sequence ID" value="XM_015188663.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D10"/>
    <dbReference type="STRING" id="46245.Q29D10"/>
    <dbReference type="GeneID" id="4812724"/>
    <dbReference type="KEGG" id="dpo:4812724"/>
    <dbReference type="eggNOG" id="KOG0277">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046581_1_0_1"/>
    <dbReference type="OMA" id="FAVHWNL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079626">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29D10">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001253">
        <property type="entry name" value="Peroxin 7, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="116"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001353103.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015044149.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="37463" checksum="E014A100C8CFBAD5" modified="2008-10-14" version="2">MQSKTHTTTDRHGYSLRFSPFEANYLLLATSQLYGLAGGGSLFLLSQNSTSDGQSPNLSELCRLEWSDGLFDVAWCPYAADVAATASGDGSLQIWSGLDAEAAEGQETPKQPLICLQEHKNEIYSLDWGEQWNYHTLLSASWDRTLKLWDCNRQHSITTFVGHSDLIYCAKFSPLIANLFASVSTDGQLNLWNSLDFAGKPLMSIEAHASEVLACDWSHFDRNILVTGGSDGLIRGWDLRKMRTHVFELYSGEFAVRRLACSPHSSTVLASANYDFTTRIWNLERGESPQEINEQHTEFVCGLDWNPHRAHQLADCGWDSLANVYTPKCLIQDGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D61</accession>
    <accession>A0A6I8UBZ1</accession>
    <name>Q29D61_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 66 member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4812668</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353052.2">
        <property type="nucleotide sequence ID" value="XM_001353016.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D61"/>
    <dbReference type="FunCoup" id="Q29D61">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29D61"/>
    <dbReference type="GeneID" id="4812668"/>
    <dbReference type="KEGG" id="dpo:4812668"/>
    <dbReference type="eggNOG" id="KOG3211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053568_3_0_1"/>
    <dbReference type="InParanoid" id="Q29D61"/>
    <dbReference type="OMA" id="YALIYYA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071786">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29D61">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016817">
        <property type="entry name" value="MannP-dilichol_defect-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226:SF3">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 66 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023381">
        <property type="entry name" value="MannP-dilichol_defect-1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR023381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353052.2"/>
        </source>
    </evidence>
    <sequence length="221" mass="24831" checksum="4BB393ED0953BF25" modified="2008-10-14" version="2">MSGGLSEVVSEYLDKGLVLVIADLLSLITVSSCLVIKVPQINTIRENKSSKGISVLGLCLELFSYTVMISYNYTSDYDFLSYMEYPVLLLQEYVLIYYAFKYQDLLGKKTQIVAIAYVIIASLIYLKLFPIIILTFLVPFCTPIGATSKVLQLLAILRTKDASSVSRTTWALSAFTNMTRIYTVYVQSHDWMLLSNFLISTFLSASVFAAACFYKKKVKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EJ6</accession>
    <accession>A0A6I8UAX7</accession>
    <name>Q29EJ6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fat body protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Pdh</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352567.2">
        <property type="nucleotide sequence ID" value="XM_001352531.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EJ6"/>
    <dbReference type="SMR" id="Q29EJ6"/>
    <dbReference type="FunCoup" id="Q29EJ6">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EJ6"/>
    <dbReference type="GeneID" id="4811865"/>
    <dbReference type="KEGG" id="dpo:4811865"/>
    <dbReference type="eggNOG" id="KOG4169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_16_1"/>
    <dbReference type="InParanoid" id="Q29EJ6"/>
    <dbReference type="OMA" id="TDVARCM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078514">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29EJ6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000149">
        <property type="entry name" value="15-hydroxyprostaglandin dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF8">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01167">
        <property type="entry name" value="INSADHFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001352567.2"/>
        </source>
    </evidence>
    <sequence length="261" mass="28239" checksum="6B31B81938E1240E" modified="2008-10-14" version="2">MSFRGKNAVVTGGAGGIGLQVSKQLLTAGAAKVAIIDIQDNLEEFVKLRAAHPTQSVMIVKMDVANKKGVEATYEEIAKTFGSIDIVVNVAGIFNDKDVQRTLLVNLGGIINSTLSALPYMSKDNGGKGGIVINMSSVVGLDPMFIIPVYGATKAGILNFTRCLANDKYYARSGIKFVTVCPGATMTDMFTNFTEKIIFPETSDETYRILDRLNKQSAADVSRCILSVLEKDKNGAVYVIEGKRVFPMELKPQWTGKEQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FJ4</accession>
    <accession>A0A6I8UEG5</accession>
    <name>Q29FJ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein tumorous imaginal discs, mitochondrial isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4814132</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354208.3">
        <property type="nucleotide sequence ID" value="XM_001354172.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FJ4"/>
    <dbReference type="SMR" id="Q29FJ4"/>
    <dbReference type="FunCoup" id="Q29FJ4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29FJ4"/>
    <dbReference type="GeneID" id="4814132"/>
    <dbReference type="KEGG" id="dpo:4814132"/>
    <dbReference type="eggNOG" id="KOG0715">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_1"/>
    <dbReference type="InParanoid" id="Q29FJ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080311">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051938">
        <property type="entry name" value="Apopto_cytoskel_mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44145:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY A MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001354208.3"/>
        </source>
    </evidence>
    <sequence length="451" mass="50877" checksum="EE6AD02ED06D5B92" modified="2016-01-20" version="3">MVKRSSLLISGLFILNFGKNMSRSMSLCLLSNRALLRRRITLAGLYTGRCPLKAERGYAAYQFKQPKTFYYPDRKQEAERKSGVATEQAQRQPEDGGKPKDFYYKVLGVGKYATSKEIRNAYLMLAKRYHPDKMSSGRSLKHFQEISNAYHILTDENKRLEYDQLGGVKDEKAFLEQATVNPTILGQRLDEAKKLQAETDANDEINNLKQLKGKSFELSLSFEEAANGCKKRVNIRYLRKCGKCGGKSQLMTHREVGKEPCRRCNGAGKVTKRTVTFTAEKTCEHCKGKGFINRRDCEPCGNRGFIASGVEVLVKVPPGSKDGDVITIQNPNTQERVNYRLVVERSDYFRRDGLNVHSDKLITISQAILGGSFTVRGLHEPVEIRVDPGTQTNSEIIIKSKGIRCENGVGNHVVTLKVFIPTKLSMKQRLLILSLAQAEDPIFERQNLKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GX0</accession>
    <accession>A0A6I8UG23</accession>
    <name>Q29GX0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCT-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CCT2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354933.2">
        <property type="nucleotide sequence ID" value="XM_001354897.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GX0"/>
    <dbReference type="SMR" id="Q29GX0"/>
    <dbReference type="FunCoup" id="Q29GX0">
        <property type="interactions" value="2543"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GX0"/>
    <dbReference type="GeneID" id="4814653"/>
    <dbReference type="KEGG" id="dpo:4814653"/>
    <dbReference type="eggNOG" id="KOG0363">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_6_2_1"/>
    <dbReference type="InParanoid" id="Q29GX0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080042">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29GX0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03336">
        <property type="entry name" value="TCP1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000002">
        <property type="entry name" value="T-complex protein 1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000045">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012716">
        <property type="entry name" value="Chap_CCT_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02341">
        <property type="entry name" value="chap_CCT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001354933.2"/>
        </source>
    </evidence>
    <sequence length="535" mass="57901" checksum="1D34C6751235D241" modified="2016-01-20" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q29H81</accession>
    <accession>A0A6I8UG01</accession>
    <name>Q29H81_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Plasminogen receptor (KT)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4814498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354822.2">
        <property type="nucleotide sequence ID" value="XM_001354786.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29H81"/>
    <dbReference type="FunCoup" id="Q29H81">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29H81"/>
    <dbReference type="GeneID" id="4814498"/>
    <dbReference type="KEGG" id="dpo:4814498"/>
    <dbReference type="eggNOG" id="KOG4544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115107_0_0_1"/>
    <dbReference type="InParanoid" id="Q29H81"/>
    <dbReference type="OMA" id="MGYYTDW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072307">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019319">
        <property type="entry name" value="Plg-R(KT)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13411">
        <property type="entry name" value="PLASMINOGEN RECEPTOR (KT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13411:SF6">
        <property type="entry name" value="PLASMINOGEN RECEPTOR (KT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10166">
        <property type="entry name" value="DUF2368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354822.2"/>
        </source>
    </evidence>
    <sequence length="154" mass="18022" checksum="1F8BE9904E71B186" modified="2008-10-14" version="2">MGATASRKKTPGSGHPEHDDPAYRRCQELKMERWIQMHYQIKQREQALAIAQHRELFYWLGGFYLSAIYGSASYYQRTKRLSVLAPLLPLSFVMGYYTDWAYGSKLHRIQAEANMIMEHEQELLHWPGGLPSVASIDEARMENEMEKKMHPHHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LU8</accession>
    <accession>A0A6I8UKL7</accession>
    <name>Q29LU8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4817404</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356881.1">
        <property type="nucleotide sequence ID" value="XM_001356845.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LU8"/>
    <dbReference type="FunCoup" id="Q29LU8">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LU8"/>
    <dbReference type="GeneID" id="4817404"/>
    <dbReference type="KEGG" id="dpo:4817404"/>
    <dbReference type="eggNOG" id="ENOG502S5PS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126345_1_1_1"/>
    <dbReference type="InParanoid" id="Q29LU8"/>
    <dbReference type="OMA" id="VIRTCGF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080578">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LU8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032222">
        <property type="term" value="P:regulation of synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031424">
        <property type="entry name" value="QVR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050975">
        <property type="entry name" value="Sleep_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562">
        <property type="entry name" value="ATILLA, ISOFORM B-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33562:SF14">
        <property type="entry name" value="PROTEIN QUIVER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17064">
        <property type="entry name" value="QVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356881.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16054" checksum="DA485EBB531A00FA" modified="2006-04-04" version="1">MQYIYVISALILAVAVQQGYAIKCFVCNSHKDANCALDIPPENLLKDCDEQYSSRGKGIPTYCRKITQIIEFSVNSLPPDSRVIRTCGYQNQTSTNYCYSRAGFGGRQVVCSCDTDNCNGAATMSASTVGVVAMVGLLLGAFQWLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q29M99</accession>
    <accession>A0A6I8UJY2</accession>
    <name>Q29M99_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4817193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356730.1">
        <property type="nucleotide sequence ID" value="XM_001356694.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29M99"/>
    <dbReference type="SMR" id="Q29M99"/>
    <dbReference type="GeneID" id="4817193"/>
    <dbReference type="KEGG" id="dpo:4817193"/>
    <dbReference type="eggNOG" id="ENOG502S3Z4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_795175_0_0_1"/>
    <dbReference type="InParanoid" id="Q29M99"/>
    <dbReference type="OMA" id="PEKFCPI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073887">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46829">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46829:SF1">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356730.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="41175" checksum="87ABE33D6EB55045" modified="2006-04-04" version="1">MPYLYGIADTKFLVKKIKKKVSTGVPPTKFRRNEFLKGRPKIDLNDVPIQFRASPASLVELCVNVVDKLPIPSIFEWDDMDVRRWIKRYGYPEYMNTFRVNMIWGRKLLLVDACALSAMNIKDFDHIRHITYGIRMLFHFELAKFSRSISLPDENPNELYLLFHTQTGVNYDEVRRSDLYRRMQLIRERSKNLDHWDLLYLWLRREREYNYTELIGMIPRFNLYKCKKALPVAPPEIHPEDVMCKVCIPPCDCNWTDRDLRLPWRLNVLTPQLTLTRSKWNAMEKVCTVCIPPCECRWPPRYYLTGTVIKCLQTKFPEKFCPIFDDRLHVSARPSLVERWTRFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PC9</accession>
    <accession>A0A6I8ULH9</accession>
    <name>Q29PC9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cytochrome c oxidase subunit 5B, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COX5BL</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357295.1">
        <property type="nucleotide sequence ID" value="XM_001357259.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PC9"/>
    <dbReference type="FunCoup" id="Q29PC9">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29PC9"/>
    <dbReference type="GeneID" id="4817919"/>
    <dbReference type="KEGG" id="dpo:4817919"/>
    <dbReference type="eggNOG" id="KOG3352">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1733429_0_0_1"/>
    <dbReference type="InParanoid" id="Q29PC9"/>
    <dbReference type="OMA" id="RSAKWMW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070780">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00924">
        <property type="entry name" value="Cyt_c_Oxidase_Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.11.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002124">
        <property type="entry name" value="Cyt_c_oxidase_su5b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036972">
        <property type="entry name" value="Cyt_c_oxidase_su5b_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01215">
        <property type="entry name" value="COX5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51359">
        <property type="entry name" value="COX5B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602124-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001357295.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="16760" checksum="76BAF2EA6E3095A0" modified="2006-04-04" version="1">MSSYLARLLKAQTASKRLVSQLVQRKGKLSSLLQRVGLKKWTDTAAARAISTTPVLKAEMADDMELATGIAKREMQLKEQGCGDPWGLGKILKRGSGRLNDPTEIPSAFDGRLVGCLCLDDRFAKWMWLEEGPPKRCECGHYFVLKKVPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P5C2</accession>
    <accession>A0A6I8VIH9</accession>
    <name>A0A0R3P5C2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">AnxB10</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041820.1">
        <property type="nucleotide sequence ID" value="XM_015186334.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P5C2"/>
    <dbReference type="SMR" id="A0A0R3P5C2"/>
    <dbReference type="FunCoup" id="A0A0R3P5C2">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P5C2"/>
    <dbReference type="GeneID" id="4814884"/>
    <dbReference type="InParanoid" id="A0A0R3P5C2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243578">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3P5C2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000010">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF177">
        <property type="entry name" value="ANNEXIN B10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015041820.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="35604" checksum="F50E8121522F8A94" modified="2016-01-20" version="1">MDYKPVPTVVGATPFDAAADAQTLRAAMKGLGTDEQEIIDVLASRSNGQRQLIRSVYDTEFERDLVDDLKSELGGKFEDVIVAMMMPPVEYLCKQLHSAMAGMGTEESTLVEILCTKSNEEMHQIVEAYEDKYQRPLAEQMCSETSGFFRRLLTLIVTGVRDGLNTPVDAAEAKEQASQLYAAGEAKLGTDEEVFNRIMSHASFPQLRLVFEEYKELSGQTIEQAIKHEMADELHEAMMAIVECVQSPAAFFANRLYKAMNGAGTDDSTLIRIIVCRSEIDLETIKQEFERIYNRTLLSAVVDAETSGDYKRALTALLGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UAC4</accession>
    <accession>Q29CR8</accession>
    <name>A0A6I8UAC4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">LOC4811769</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12 13">MV-25-SWS-2005</strain>
            <tissue evidence="12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C65 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="RefSeq" id="XP_001352338.1">
        <property type="nucleotide sequence ID" value="XM_001352302.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235987.1">
        <property type="nucleotide sequence ID" value="XM_033380096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UAC4"/>
    <dbReference type="SMR" id="A0A6I8UAC4"/>
    <dbReference type="GeneID" id="4811769"/>
    <dbReference type="KEGG" id="dpo:4811769"/>
    <dbReference type="OMA" id="ADHVQIT"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078562">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22763">
        <property type="entry name" value="OTUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.20:FF:000001">
        <property type="entry name" value="Ubiquitin thioesterase OTUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.60">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.20">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016615">
        <property type="entry name" value="Otubain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042468">
        <property type="entry name" value="Peptidase_C65_otubain_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042467">
        <property type="entry name" value="Peptidase_C65_otubain_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013503">
        <property type="entry name" value="Ubiquitin_thioesterase_Otubain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="10">
        <location>
            <begin position="64"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with free ubiquitin" evidence="9">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with free ubiquitin" evidence="9">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with free ubiquitin" evidence="9">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with free ubiquitin" evidence="9">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with free ubiquitin" evidence="9">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR013503-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR013503-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001352338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033235987.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="30525" checksum="3493A7C263C448BF" modified="2020-08-12" version="1">MESFKQNDGNRDELIIQQQRDIEKEISDSTPLVGELLPLTCLWTEYNGDDVFTAKIQDLAKKYKFIRRTRPDGNCFFRAFAYSYMEYLISNSSAYKEFQKLAEDSKDKLVELGFPSFTLEDFHETFMEVIRRVSPDNPGGYNKVQNELHKILNEQGYSDYVVVYLRLITSGKLQEEAEFYQNFIEGDFTIEEFRHQEVEPMFKESDHIHIIALCAALGAGVRVEYLDRGEGCIVKAHDFPEGTEPTIYLIYRPGHYDILYPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBG4</accession>
    <name>A0A6I8UBG4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4812136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352833.3">
        <property type="nucleotide sequence ID" value="XM_001352797.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBG4"/>
    <dbReference type="FunCoup" id="A0A6I8UBG4">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812136"/>
    <dbReference type="InParanoid" id="A0A6I8UBG4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBG4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038929">
        <property type="entry name" value="CCDC13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31935">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31935:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="304"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="464"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="577"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352833.3"/>
        </source>
    </evidence>
    <sequence length="659" mass="74844" checksum="2295E47319B6A3A0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBU9</accession>
    <name>A0A6I8UBU9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fal</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAMHD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352941.3">
        <property type="nucleotide sequence ID" value="XM_001352905.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBU9"/>
    <dbReference type="FunCoup" id="A0A6I8UBU9">
        <property type="interactions" value="805"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812365"/>
    <dbReference type="InParanoid" id="A0A6I8UBU9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBU9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="2">
        <location>
            <begin position="85"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001352941.3"/>
        </source>
    </evidence>
    <sequence length="407" mass="46993" checksum="7355CDDE747A1B8A" modified="2020-08-12" version="1">MSASLILSGDYRDHGAQQPCQQQQQLLHQQAPLHSVLPYMLIEDEVHGVIELPSHIQEIVEHPLFQRLKKIHQLGLVAWVIAPHANQKRYDHCLGTYKSAQEHLRAIERNSKYETKLPDWCRKAVEIAALLHDIGHGPMSHAWEKASHNKFNHEENALDCVDRIFNDARHPELVALRDEGSGRGVRLIKALILGDTEYLNFPMMGHAYIFDIVHNNRCGLDVDKWDYLRRDNKRLKILGPEEMDFDNIFLKSRISQDGQRIEYRYDDYHRIYTLFAARARLHVEAYQHPKNAAAEKIFVDLVQRLAPQMFDLRSKDAGWLNLDDDHILEMVKNDPMAAYIREPQRVVELPSLDCLGANALPVSRIIEGPGSTMKPDKAFALYGDKRKKRKINRCLSATTFNKCFTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBX8</accession>
    <name>A0A6I8UBX8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein CtIP isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CtIP</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352979.2">
        <property type="nucleotide sequence ID" value="XM_001352943.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBX8"/>
    <dbReference type="FunCoup" id="A0A6I8UBX8">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812281"/>
    <dbReference type="InParanoid" id="A0A6I8UBX8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UBX8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001352979.2"/>
        </source>
    </evidence>
    <sequence length="501" mass="56427" checksum="B7F8DBF73C02A149" modified="2020-08-12" version="1">MTCGACRGRKDKGIKCLIAALDTIKEHALMMQHEAEEKDKTIGSLQAQNEKLHRAVDLLKERQESLAVLHDDKRRKVSPKKSKEDISPLPQSQSSLNMNIALNSIELSDEDTEEQEDESIAETEAPSIGSPRKLRPHHRKPDVRNLENVQPNNVTSVSQSWLRKTPKNIGMMTKLSLTHRGSSLQFKQTRLKFDGNKASGSEKDVIEESPNPSPGSKRAPASRSLLQRADTGNALNEDNFVLSRSNSVNIKNTDPSFHMDVEKFIGIDSLPDVSLELPVMPPPATPPHLKIDNSSESVVILTPATQDIIFVNDSSEDIKKIGTMDVLAEIMKEDDDACSSALLQYEKIMIDQQKHVQPNPQKVHTALGPVGAIKDEPITQPNEDHGNESTRLSEDIEKYMMEEDDSKESDDEIPRPIMVKEEPELTLKERFNIECEECEKFINFMGSNLNDEKIQLYLSKCKHNNERTGSPPGFWNPHMVSFAEDDPRNEVLIDTRFRDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDE3</accession>
    <name>A0A6I8UDE3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4813862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353768.3">
        <property type="nucleotide sequence ID" value="XM_001353732.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDE3"/>
    <dbReference type="FunCoup" id="A0A6I8UDE3">
        <property type="interactions" value="331"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813862"/>
    <dbReference type="InParanoid" id="A0A6I8UDE3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UDE3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353768.3"/>
        </source>
    </evidence>
    <sequence length="390" mass="41585" checksum="248BB1E1BACE70F1" modified="2020-08-12" version="1">MHSNVNQGGYVPPGFGAGYQPNAPPAEGYGYPPPAAPNHPYAPSPHHQPPGQPPMNYGFVPGQQPQGYAPVPQMPPYGEAPPYGAGQPPYMGGGGGGGGPSPQQSFGYPPGPAGPPQHFGYPPGPAGAPQQPIVTQPGMPPAQPQPGMGPGMGGGDWMSIPAGIPNCPRGLEYLTTIDQLLVKQKVELLEAFTGFETNNKFSIKNALGQKVYYAVEDNDCCTRNCCGPSRPFEMKVFDNYRQEVIHMHRPLACSACCFPCCLQSIEVSAPPGNVIGTIEQEWSICSPSFRILNHIGDTVLRIEGPLCTYSLCGDVEFNVVSLTGEKIGKISKQWSGLAREIFTDADFFGISFPLDLDVRMKAVLLGATFLIDAMFFEKSGNRETDGPGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE06</accession>
    <name>A0A6I8UE06_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4813547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354007.3">
        <property type="nucleotide sequence ID" value="XM_001353971.4"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813547"/>
    <dbReference type="InParanoid" id="A0A6I8UE06"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="766"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="885"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1269"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="615"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="776"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="890"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1274"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1291"/>
            <end position="1300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354007.3"/>
        </source>
    </evidence>
    <sequence length="1300" mass="143875" checksum="C5F157E624536CC1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UF45</accession>
    <name>A0A6I8UF45_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">COA8 family protein CG14806, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Coa8</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COA8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354515.3">
        <property type="nucleotide sequence ID" value="XM_001354479.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UF45"/>
    <dbReference type="FunCoup" id="A0A6I8UF45">
        <property type="interactions" value="696"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815152"/>
    <dbReference type="InParanoid" id="A0A6I8UF45"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018796">
        <property type="entry name" value="COA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107">
        <property type="entry name" value="APOPTOGENIC PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10231">
        <property type="entry name" value="COA8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001354515.3"/>
        </source>
    </evidence>
    <sequence length="172" mass="20650" checksum="BA32BD4E885FAF02" modified="2020-08-12" version="1">MCFKYQTRILFTQFNLPRCYATKQAPKGSRPVGLAHPPDPKTVSCDYIGPPDPQSNLRPYVRHYDENETDLAKKLRLTRIEVEAWNASFWTKHNKRFYEEKEDFIRLHKEAGTEDISADRMSEFYKTFLDKNWRIHMMYNLSWYLKNFDMLTLAFMVQVQKLLSRRKRRTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UGD1</accession>
    <name>A0A6I8UGD1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calpain-C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CalpC</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355059.3">
        <property type="nucleotide sequence ID" value="XM_001355023.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UGD1"/>
    <dbReference type="FunCoup" id="A0A6I8UGD1">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815308"/>
    <dbReference type="InParanoid" id="A0A6I8UGD1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UGD1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000300">
        <property type="entry name" value="Calpain-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000018">
        <property type="entry name" value="Calpain-c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF394">
        <property type="entry name" value="CALPAIN-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="3">
        <location>
            <begin position="18"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="537"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001355059.3"/>
        </source>
    </evidence>
    <sequence length="666" mass="76374" checksum="F74130F0EF2002F9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UI81</accession>
    <name>A0A6I8UI81_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein msb1l</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">msb1l</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355819.3">
        <property type="nucleotide sequence ID" value="XM_001355783.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UI81"/>
    <dbReference type="FunCoup" id="A0A6I8UI81">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816134"/>
    <dbReference type="InParanoid" id="A0A6I8UI81"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UI81">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355819.3"/>
        </source>
    </evidence>
    <sequence length="385" mass="42600" checksum="EE0550533743CD53" modified="2020-08-12" version="1">MGAKASKIEGEYLPDTPTLNKLRLASFKAENVEMTTPNKENNALLDPRSPNGCRTPLNFLQSQPQAQTLRAADHPSAPTENAFSQLRKRLLRGFSLNDPRSPQLNRTPLILDEVRSLNLDDTFADLFVDTRAPARRIAETPAANIIESPYQSLSSPSAETGDVSCDSFGTPPTHHNNSSLEIVSLPYDEEETMPCTAELPATPAAVQGHYDPRSPSIGVERTPIIFCDDEEEEARETEMLEQILETLTLNLSAESSMASFASAGTDTNLEEPPKPHSSDKQPANKHLDRVRLGNKRRVAPRKPARASKTKIYMDKESSPSVASTPKTTPNATPKRTPLSCVKNRAHLRSRSVERELRKSQLPLHSFDDQLNPTEYRFNGSIGTMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UI96</accession>
    <name>A0A6I8UI96_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial 2-oxodicarboxylate carrier</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Solute carrier family 25 member 21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC4816211</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="23">MV-25-SWS-2005</strain>
            <tissue evidence="23">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Transports dicarboxylates across the inner membranes of mitochondria by a counter-exchange mechanism. Can transport 2-oxoadipate (2-oxohexanedioate), 2-oxoglutarate, adipate (hexanedioate), glutarate, and to a lesser extent, pimelate (heptanedioate), 2-oxopimelate (2-oxoheptanedioate), 2-aminoadipate (2-aminohexanedioate), oxaloacetate, and citrate. Plays a central role in catabolism of lysine, hydroxylysine, and tryptophan, by transporting common metabolite intermediates (such as 2-oxoadipate) into the mitochondria, where it is converted into acetyl-CoA and can enter the citric acid (TCA) cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2-oxoadipate(in) + 2-oxoglutarate(out) = 2-oxoadipate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71739"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2-oxoheptanedioate(in) + 2-oxoglutarate(out) = 2-oxoheptanedioate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71755"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:72701"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-2-aminoadipate(in) + 2-oxoglutarate(out) = L-2-aminoadipate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71747"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>citrate(in) + 2-oxoglutarate(out) = citrate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71763"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>glutarate(in) + 2-oxoglutarate(out) = glutarate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71751"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30921"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>heptanedioate(in) + 2-oxoglutarate(out) = heptanedioate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71759"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:36165"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hexanedioate(in) + 2-oxoglutarate(out) = hexanedioate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71743"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17128"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355836.3">
        <property type="nucleotide sequence ID" value="XM_001355800.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UI96"/>
    <dbReference type="FunCoup" id="A0A6I8UI96">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816211"/>
    <dbReference type="InParanoid" id="A0A6I8UI96"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 20 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 21" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="20">
        <location>
            <begin position="16"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="20">
        <location>
            <begin position="115"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="20">
        <location>
            <begin position="210"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_001355836.3"/>
        </source>
    </evidence>
    <sequence length="305" mass="33764" checksum="477B5BC153072C83" modified="2020-08-12" version="1">MGPQVEPEWEPMPGKSRALFQFAAGGSAGLLELLLLHPLDVVKTRLQLQGVQAAAGELRYTGVFDCISKIYSHEGVAAFWKGIVPPICSETPKRAAKFVVYEQAKPLFLFGALQPTALTHAMAGALAGTLECFFLNPFEVVKITQQADRSDSLNTLTVARRIVQTDGYGIKGLYRGITALMIRNAIFHFAYFGIYQGLKDEIPAYQHDLAEFFRRGAIGFFSSSISCLFSTTFDVAKSRIQGPQPTKGSIKYKWTVPTIKTIYEEEGFRALYKGLSVKIMRAGPGGAILMLAYEYGYEYLTYKYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIV4</accession>
    <name>A0A6I8UIV4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Thyrotropin-releasing hormone-degrading ectoenzyme isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC4816294</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="22">MV-25-SWS-2005</strain>
            <tissue evidence="22">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356176.3">
        <property type="nucleotide sequence ID" value="XM_001356140.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIV4"/>
    <dbReference type="FunCoup" id="A0A6I8UIV4">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816294"/>
    <dbReference type="InParanoid" id="A0A6I8UIV4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UIV4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000008">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF253">
        <property type="entry name" value="AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026219718" evidence="17">
        <location>
            <begin position="34"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="20">
        <location>
            <begin position="49"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="18">
        <location>
            <begin position="284"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="19">
        <location>
            <begin position="578"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_001356176.3"/>
        </source>
    </evidence>
    <sequence length="715" mass="82214" checksum="1F1F90250576849F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKG6</accession>
    <name>A0A6I8UKG6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Reticulon-4-interacting protein 1 homolog, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4816896</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356824.3">
        <property type="nucleotide sequence ID" value="XM_001356788.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKG6"/>
    <dbReference type="FunCoup" id="A0A6I8UKG6">
        <property type="interactions" value="980"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816896"/>
    <dbReference type="InParanoid" id="A0A6I8UKG6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UKG6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08248">
        <property type="entry name" value="RTN4I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000147">
        <property type="entry name" value="Reticulon-4-interacting protein 1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037397">
        <property type="entry name" value="RTN4IP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="62"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001356824.3"/>
        </source>
    </evidence>
    <sequence length="411" mass="44292" checksum="9835E6A5EAF2B900" modified="2020-08-12" version="1">MQSTRYNGVIWMRQCVLHARRTYSVSANVTSAGNNTKGEHLPPTTPTKSLEKMRGWQLHSYGDIDELLLSEKLKIPQIRSSNECLVRVRTTAVNPIDLAMLRGYGATVLNKLRCQPGDDIEFPLTLGREFCGELVQKGMGVTSHSLKLGARVWGVVPVHASPGAHAEYVAVPSYCLAPAPKQLDDSEAASVLYAGLTAWSGLYISGGLGGPCGATTAAGGGAHKRILVLGGSGGVGTLAIQILKSQGAQVLATCSENAVEMVRCLGADFVVDYKSSDAMDELCKFAPYDIVLDCAGHGGAHASELKFDFRQYITFSSPLLANIDQQGLGLGAIKNMVDLVQTNVKSLTQRGGMVKWGFFNPAPHGIAFLEQLVRQRKLIPLIDSTFSFEEMPKAFERMKCGHLRGKIVVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ34</accession>
    <name>A0A6I8UQ34_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transforming growth factor beta regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4802124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359105.3">
        <property type="nucleotide sequence ID" value="XM_001359068.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ34"/>
    <dbReference type="FunCoup" id="A0A6I8UQ34">
        <property type="interactions" value="861"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802124"/>
    <dbReference type="InParanoid" id="A0A6I8UQ34"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003889">
        <property type="entry name" value="FYrich_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003888">
        <property type="entry name" value="FYrich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040092">
        <property type="entry name" value="TBRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22715">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA REGULATED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22715:SF0">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05965">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05964">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00542">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00541">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51543">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51542">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359105.3"/>
        </source>
    </evidence>
    <sequence length="283" mass="32608" checksum="93E28E8E04D0263A" modified="2020-08-12" version="1">MKSLSKPVQPVMHFNYKRKYDRLKRCIKLYILENSCLTDEICHLQSELAATRYQRIYLIKRLMFYEGLEKANIEPNCVSNGKLESNDNFLRALNKNKQSVSYPREINKDTQNGSTAQLKKSFPIHLNNLLLHSLGDISANLNFHNEGWIYPVGYVATRIYAHPKDPYKKCVYTCKILNNAGVPQFQIIPDNDLDGVYFGETPNMCHAELLNTIQRYANKRVKIPFDVQGESFFGLSNPKVQSLLRLDPGFHRCLNFKGYNNIQNPTSLNNASLSFETLQTFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQU1</accession>
    <name>A0A6I8UQU1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ubc6</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="RefSeq" id="XP_001359417.1">
        <property type="nucleotide sequence ID" value="XM_001359380.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQU1"/>
    <dbReference type="SMR" id="A0A6I8UQU1"/>
    <dbReference type="FunCoup" id="A0A6I8UQU1">
        <property type="interactions" value="2302"/>
    </dbReference>
    <dbReference type="GeneID" id="4802508"/>
    <dbReference type="KEGG" id="dpo:4802508"/>
    <dbReference type="InParanoid" id="A0A6I8UQU1"/>
    <dbReference type="OMA" id="DHKSQYI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075209">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000004">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359417.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="17152" checksum="CC4B35992E4A9220" modified="2020-08-12" version="1">MSTPARRRLMRDFKRLQEDPPTGVSGAPTDNNIMIWNAVIFGPHDTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKVFHPNVYADGGICLDILQNRWSPTYDVSAILTSIQSLLSDPNPNSPANSTAAQLYKENRREYEKRVKACVEQSFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US97</accession>
    <name>A0A6I8US97_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Synaptic plasticity regulator PANTS</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Plasticity-associated neural transcript short</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4803420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Synaptic cleft</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0545 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360135.1">
        <property type="nucleotide sequence ID" value="XM_001360098.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US97"/>
    <dbReference type="FunCoup" id="A0A6I8US97">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="GeneID" id="4803420"/>
    <dbReference type="KEGG" id="dpo:4803420"/>
    <dbReference type="InParanoid" id="A0A6I8US97"/>
    <dbReference type="OMA" id="CHLYKDE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074050">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043083">
        <property type="term" value="C:synaptic cleft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052:SF1">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360135.1"/>
        </source>
    </evidence>
    <sequence length="156" mass="18180" checksum="181E849B0804CEBC" modified="2020-08-12" version="1">MGNKVENSDTKDAQNKTALDDAWAIRPCHLYKDEYDDCTSFKARFHQYFIFGQDSDCSQWLTDYKNCERYHQSNGNDVASGAAVIKSEEKRRQTRLRAHFANNTWEKRKQPPADWAQPLPEWLEKRNENTYLELKQKELSGLSVPQDEKGSLCAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USG5</accession>
    <name>A0A6I8USG5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4803300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360065.3">
        <property type="nucleotide sequence ID" value="XM_001360028.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USG5"/>
    <dbReference type="FunCoup" id="A0A6I8USG5">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803300"/>
    <dbReference type="InParanoid" id="A0A6I8USG5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000001">
        <property type="entry name" value="Takeout-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF32">
        <property type="entry name" value="CIRCADIAN CLOCK-CONTROLLED PROTEIN DAYWAKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026321564" evidence="4">
        <location>
            <begin position="22"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360065.3"/>
        </source>
    </evidence>
    <sequence length="260" mass="30803" checksum="92019B9A847845A6" modified="2020-08-12" version="1">MGMRDIFTLVLCLQLIKSLQGQILPKDIPKCRFGDSDCIVDSMNKVIRRYPRGIPALGMKPIDVVDIRNSKFWDNPKVGAFWLQFNLFDQVNYGFENTTITKVKGFTKNPTSSLMEIHGRIPSLIHKGRYMSQGRVWIVELNSTGEQLSDFQNFRFVLKLKVIMEYRNNKRYLKIYELNPIVNMDRWVFWLDNFFSENTDLTIAINQVFNIHWVEFWNELEPTNLRIFASVFRDLIEDILYKVSYDDMFLPDEKETRDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USN6</accession>
    <name>A0A6I8USN6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc transporter ZIP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Zip42C.1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360148.3">
        <property type="nucleotide sequence ID" value="XM_001360111.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USN6"/>
    <dbReference type="FunCoup" id="A0A6I8USN6">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803433"/>
    <dbReference type="InParanoid" id="A0A6I8USN6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081765">
        <property type="expression patterns" value="Expressed in multicellular organism and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF203">
        <property type="entry name" value="FI18611P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360148.3"/>
        </source>
    </evidence>
    <sequence length="373" mass="40297" checksum="D0C4C634E0B69FC3" modified="2020-08-12" version="1">MLAFRYGNMNGLASMEESGIAMGRTASPLVDRHGLMVAKVVAMVVLIVITVICGSLPYMLDRCLEWTKKSPEETRSSTAVRCLLFFGGGVLICTTFLHMLPEVIEVVEKLQSCGVLANTPFALPEMLLCTGFFLMYALDELMNAFVHRHQKKLSRKESVASCAFERGHSIRHSILVCKGVETNATPEEPKTLQEEQPKDHIGHSHMPVVAGKDSAGSSMRGLGIILALSLHELFEGMAIGLESTVSTVWFMFAAVAAHKLVLAFCVGMELLVARTRSSLAIIYLITFSIVTPIGIGVGIGISQNVEPNQPSVPSGILQGIASGTLLYVVFCEILTENHAGWRAYIAALAGFALMFGLQILSDEAEGDDSLACF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTA1</accession>
    <name>A0A6I8UTA1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Anaphase-promoting complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">APC10</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the APC10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360579.1">
        <property type="nucleotide sequence ID" value="XM_001360542.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTA1"/>
    <dbReference type="FunCoup" id="A0A6I8UTA1">
        <property type="interactions" value="1209"/>
    </dbReference>
    <dbReference type="GeneID" id="4803939"/>
    <dbReference type="KEGG" id="dpo:4803939"/>
    <dbReference type="InParanoid" id="A0A6I8UTA1"/>
    <dbReference type="OMA" id="DDCMDTY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071049">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08366">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000019">
        <property type="entry name" value="Anaphase-promoting complex subunit 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016901">
        <property type="entry name" value="APC10/Doc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028841">
        <property type="entry name" value="APC10_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="9">
        <location>
            <begin position="9"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR028841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001360579.1"/>
        </source>
    </evidence>
    <sequence length="195" mass="22266" checksum="1B0B64019D9C281B" modified="2020-08-12" version="1">MTSSMDEDIPINPPPTSEEDPLAEERLGLVREVGAQAVWSLSSCKPGFGVERLRDNIMDTYWQSDGQLPHLVNIQFHKRTNISQIYIYTDYKLDESYTPSRISIRSGTNFNDLQELQVLDLTEPTGWVQVPIKDGNVKSIRTFMLQIAVISNHQNGRDTHMRQIRIHAPVEGKLYALEPFGKFGTVDFQKFATIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTI4</accession>
    <name>A0A6I8UTI4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SH3 domain-binding protein 5 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pcs</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3BP5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360646.3">
        <property type="nucleotide sequence ID" value="XM_001360609.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTI4"/>
    <dbReference type="FunCoup" id="A0A6I8UTI4">
        <property type="interactions" value="1469"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804016"/>
    <dbReference type="InParanoid" id="A0A6I8UTI4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007940">
        <property type="entry name" value="SH3BP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423:SF1">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05276">
        <property type="entry name" value="SH3BP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="224"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="13"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="156"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360646.3"/>
        </source>
    </evidence>
    <sequence length="471" mass="53701" checksum="E1D92A0324979D19" modified="2020-08-12" version="1">MSNTEESELDPQIQIELENLNSATDEINKLEIELEEANSTFRILLNESTRRLKLSSKKLGSCIEKARPYHEALEKAREAQIECQQAAVKFQRANEIHAAAKETVALAEQRFMSNSHEWQFDNAWQEMLNHATQKVMDAETQKADCHAEHQRRTRLFHTAEQKLQQLEDRFRRSINKSRPYFEEKQVCQDQLQTQKNRIQELQQQVAGAKSTYSTALRNLERISEDIHRQRGDYPTPPGPREPGVGAELNTPTSSALPSLPDFQMELEKCDYPSIAGSQMSLAVKQPANTAETEDEEDACDYDETGAREFRGAVDEGDLEALRQKVKILAVRPIEGGDGQQQNDVWEHELKATVDKLDHLMMLKESAKRQQTERLKSTEQRPDSLGAEALERHCDVVVKITNTTSLPVTPQHHQQEPPTPIKKLQQQLAPLPSVNVSMRELPLLARLSNELLDRSNAAIVGVRRQLRRRSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTP1</accession>
    <name>A0A6I8UTP1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ELMO domain-containing protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Dlip3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360754.4">
        <property type="nucleotide sequence ID" value="XM_001360717.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTP1"/>
    <dbReference type="FunCoup" id="A0A6I8UTP1">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804148"/>
    <dbReference type="InParanoid" id="A0A6I8UTP1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004210">
        <property type="entry name" value="BESS_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF64">
        <property type="entry name" value="DORSAL INTERACTING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51031">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="3">
        <location>
            <begin position="27"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="BESS" evidence="4">
        <location>
            <begin position="294"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001360754.4"/>
        </source>
    </evidence>
    <sequence length="336" mass="38236" checksum="C52DD835D00D3590" modified="2020-08-12" version="1">MISTKLEHKMQRYYAERETTGPEFDDRLIKLVRANPAIYDVSHPHYRRNPVRVDIWDRIANELGTSSRFLQTKWKNIRYNYLQEIKAIETGQVNPNVRKRRFTEDLSFLQNTAQTYNVKKNQTFAAPTNNGSSDNDSNSFLYPDPEHLTVDASCNYDIIELDNSEDGSNSDDNEIVPELQLMEPKMEVSIEGALNGTNSSSNNSRNNTHYSSPASSPLLTPMVVMGNGYEQDEQRQQQQQQDIKNSSLSNGEVTIEPIYKSGATRRRPAASDIMGNAAKRKAMVAPLPSLAPINDPIELYCLSLVDTLRSMPRSERERVKFEFANILKDAKYKDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUL1</accession>
    <name>A0A6I8UUL1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4804622</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Assembles a suppression complex (suppresome) by tethering SIRT1 and MDM2 to regulate composite modifications of p53/TP53. Confers both deacetylation-mediated functional inactivation, by SIRT1, and ubiquitination-dependent degradation, by MDM2, of p53/TP53, promoting a proliferative and cell survival behaviors. May play a role in the regulation of spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361155.2">
        <property type="nucleotide sequence ID" value="XM_001361118.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUL1"/>
    <dbReference type="GeneID" id="4804622"/>
    <dbReference type="KEGG" id="dpo:4804622"/>
    <dbReference type="InParanoid" id="A0A6I8UUL1"/>
    <dbReference type="OMA" id="WENDNAK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075857">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052472">
        <property type="entry name" value="MORN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001361155.2"/>
        </source>
    </evidence>
    <sequence length="305" mass="35516" checksum="C893B6BBF2B89EA8" modified="2020-08-12" version="1">MGTCLERYFCPNKPVCSTGRRSTFYFPGGGSYTGYWKCNQHQGWGVKKTAKRTAKYFSDKKKPDGQLVYEGHWVMNKRQGIGSMLRKRGAELQLIYAGLWFDDMKCGEGKQFYPDGCVYFGEWLRNRRHGLGIQWYADGGIYVGEWETGFRHGLGVLFYANGNRYEGHFARGYKNGEGVFYHMHTGQIQKGMWENDNAKTSLMQDEVLIRRNDKVTPYPITQNYLKYPNEIMRELFQRYKPFGDKPHRRFNDKVSLEFIHHRRQYASFEERIASPTMVDLYPDSGFVCTCDCAQIARGDTKVSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UV18</accession>
    <name>A0A6I8UV18_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4804726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361242.1">
        <property type="nucleotide sequence ID" value="XM_001361205.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UV18"/>
    <dbReference type="FunCoup" id="A0A6I8UV18">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="4804726"/>
    <dbReference type="KEGG" id="dpo:4804726"/>
    <dbReference type="InParanoid" id="A0A6I8UV18"/>
    <dbReference type="OMA" id="SEDYAPH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072176">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026360383" description="Secreted protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="12941" checksum="8AFA8DEE4B979D8F" modified="2020-08-12" version="1">MQCTLSLSVIAVIALIFSLNMTQGAPISSSSDTAAILSPTDLTGEAVRQKRASADYYRGDYFICYPKSEVYGNAYGGSQRRSDDSVARPPHRLSDDSFEVRKDRADARRAAYTDSYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVP9</accession>
    <name>A0A6I8UVP9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor TFIID subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Taf5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361373.4">
        <property type="nucleotide sequence ID" value="XM_001361336.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVP9"/>
    <dbReference type="FunCoup" id="A0A6I8UVP9">
        <property type="interactions" value="1309"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804885"/>
    <dbReference type="InParanoid" id="A0A6I8UVP9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.500:FF:000004">
        <property type="entry name" value="Transcription initiation factor TFIID subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000243">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="11">
        <location>
            <begin position="110"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="371"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="444"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="486"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="528"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="570"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="612"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="285"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001361373.4"/>
        </source>
    </evidence>
    <sequence length="702" mass="78910" checksum="29F984759AFF3C3E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVW3</accession>
    <name>A0A6I8UVW3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sugar phosphate phosphatase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
            <ecNumber evidence="10">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4805273</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate. Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism. Has also been shown to have O-methyltransferase activity that methylates glutamate residues of target proteins to form gamma-glutamyl methyl ester residues. Possibly methylates PCNA, suggesting it is involved in the DNA damage response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>beta-D-fructose 1-phosphate + H2O = D-fructose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:35603"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>beta-D-fructose 6-phosphate = dihydroxyacetone + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:28002"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="domain">
        <text evidence="10">Subfamily III proteins have a conserved RTxK motif about 40-50 residues from the C-terminus; the threonine may be replaced by serine or cysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the damage-control phosphatase family. Sugar phosphate phosphatase III subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EC" id="3.1.3.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001361698.3">
        <property type="nucleotide sequence ID" value="XM_001361661.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVW3"/>
    <dbReference type="FunCoup" id="A0A6I8UVW3">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805273"/>
    <dbReference type="InParanoid" id="A0A6I8UVW3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051998">
        <property type="term" value="F:protein carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10880:FF:000005">
        <property type="entry name" value="DUF89-domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039763">
        <property type="entry name" value="ARMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260:SF6">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="11">
        <location>
            <begin position="65"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001361698.3"/>
        </source>
    </evidence>
    <sequence length="471" mass="55451" checksum="9F5385AFF686BF91" modified="2020-08-12" version="1">MLSDKMDQSTELTSQTEMESAVVHSVKIEEREDLVFNEKHQIFDIAPRPHVELSGHFLRSFAYFTLKHRSPMMLGEMISFLNDYEPKIMEIFGSYAHFDLKRICWSLQLLRDEVQENQEFQLFHGKAPDVEDWNRFISTLDEDRNRWFSSVWLHAECYMYRRIWAIFRRSDLLSSYDYFSQKKIDATTSVVKLMRDVIMGMRGMLRSQEKFQRMLKLSLWSNRCDLSINKQAPDAQFLKLMADYESDLLVDQSADVWRVLTEAWDPVYVDIVCDNAGFELFSDLLLAEYLIETGLALKVRFHVKAIPWFISDVTKQDFLWMLRFLRSHKTSEIAAFGRRLRRYMRDRSFVLCDTCYFWTSPHDCSQMKKRLPCLYVSLSEAALVIFKGDLNYRKLLGDINWDYTAPLIECMRGFFPTSVCALRTIKSDIYCGLPICTVEWLSEDDPQWMRTGKKGVIQVAVMHRLSGDALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVX6</accession>
    <name>A0A6I8UVX6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RNA polymerase-associated protein Rtf1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Rtf1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361651.1">
        <property type="nucleotide sequence ID" value="XM_001361614.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVX6"/>
    <dbReference type="FunCoup" id="A0A6I8UVX6">
        <property type="interactions" value="2436"/>
    </dbReference>
    <dbReference type="GeneID" id="4805210"/>
    <dbReference type="KEGG" id="dpo:4805210"/>
    <dbReference type="InParanoid" id="A0A6I8UVX6"/>
    <dbReference type="OMA" id="ISGCYAR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070721">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.200:FF:000001">
        <property type="entry name" value="RNA polymerase-associated protein RTF1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.200">
        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115:SF8">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Plus3" evidence="10">
        <location>
            <begin position="418"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="375"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="648"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="726"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="590"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="163"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361651.1"/>
        </source>
    </evidence>
    <sequence length="764" mass="86327" checksum="9D7980A4678CD1C8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW14</accession>
    <name>A0A6I8UW14_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sugar phosphate phosphatase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
            <ecNumber evidence="10">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4805275</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate. Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism. Has also been shown to have O-methyltransferase activity that methylates glutamate residues of target proteins to form gamma-glutamyl methyl ester residues. Possibly methylates PCNA, suggesting it is involved in the DNA damage response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>beta-D-fructose 1-phosphate + H2O = D-fructose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:35603"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>beta-D-fructose 6-phosphate = dihydroxyacetone + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:28002"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="domain">
        <text evidence="10">Subfamily III proteins have a conserved RTxK motif about 40-50 residues from the C-terminus; the threonine may be replaced by serine or cysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the damage-control phosphatase family. Sugar phosphate phosphatase III subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EC" id="3.1.3.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001361700.3">
        <property type="nucleotide sequence ID" value="XM_001361663.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW14"/>
    <dbReference type="FunCoup" id="A0A6I8UW14">
        <property type="interactions" value="829"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805275"/>
    <dbReference type="InParanoid" id="A0A6I8UW14"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051998">
        <property type="term" value="F:protein carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10880:FF:000005">
        <property type="entry name" value="DUF89-domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039763">
        <property type="entry name" value="ARMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260:SF6">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="12">
        <location>
            <begin position="70"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001361700.3"/>
        </source>
    </evidence>
    <sequence length="480" mass="55626" checksum="93CC4AFCFC218017" modified="2020-08-12" version="1">MESPSLVRYGGQTEDEPKTKSHSERTDDGSNIVSRIFKTEFDAKYKIYDMRTPLNAFMCGQYKRSGAYNTLRNRLPVVLTNVIDTLTKDKSELVAEFGEAARGELKIIIGLISRLKYELQTDKPFQGFDGQEPDRALWNKFLNLLPDEERTFFRSCCLYSECYMHRKLYSFVENSIFLKPYDYHVKVKENSLTSCIDDVLSLTKYTRRTDNSLEMFGELLRISLWSNYNDTTRDPEAPPLPEFNINVLEDVSAADEFILVNQVADIWQCLDNRKQKKPCQVDFVLDNAGYELFTDIILAEFMIEKGLATKVLFHVKAHPWFVTDVTARDFKWTLEYLSQHADYIISLIGKKFLQFLAEGKFQLAPISHFWTCPYPYYRMRDTDPDLYSSLQQSKLIVFKGDLNYRKLLQDVCWDSTQDLNTCLRGFLPSNICAVRRVKAEVICGLPEGVSETLSKKDPHWMISGNYGVIQFVDGSREFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW59</accession>
    <name>A0A6I8UW59_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP synthase subunit f, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ATPsynF</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase F chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361845.2">
        <property type="nucleotide sequence ID" value="XM_001361808.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW59"/>
    <dbReference type="SMR" id="A0A6I8UW59"/>
    <dbReference type="FunCoup" id="A0A6I8UW59">
        <property type="interactions" value="630"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805444"/>
    <dbReference type="InParanoid" id="A0A6I8UW59"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019344">
        <property type="entry name" value="F1F0-ATPsyn_F_prd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13080">
        <property type="entry name" value="ATP SYNTHASE F CHAIN, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13080:SF20">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT F, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10206">
        <property type="entry name" value="WRW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001361845.2"/>
        </source>
    </evidence>
    <sequence length="107" mass="12612" checksum="2467799F53A1FABB" modified="2020-08-12" version="1">MAFGDYPAEYNPKVHGPYDPARFYGKADVPFGQVKLGELGSWFGRRNKTPNAMVGAVSRAWWRWQHKYVFPKRAGIAPFFQLTVASMTFFYMINYTKLKHHRNYKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWD1</accession>
    <name>A0A6I8UWD1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein bug</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bug</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361949.2">
        <property type="nucleotide sequence ID" value="XM_001361912.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWD1"/>
    <dbReference type="FunCoup" id="A0A6I8UWD1">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805564"/>
    <dbReference type="InParanoid" id="A0A6I8UWD1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042418">
        <property type="entry name" value="TXNDC15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14684">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14684:SF2">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026073991" evidence="2">
        <location>
            <begin position="19"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="134"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361949.2"/>
        </source>
    </evidence>
    <sequence length="301" mass="34098" checksum="555EC878868A970E" modified="2020-08-12" version="1">MNQNLLFIFFLYISRSNAELSGLLSALQYFGLYGNGSDSLGQSRGQCEYTFESFALLGDRHTCAPDDEHILHLTQLPPQRKPPIMIKCLQPEPAADNGNGTETVPKLLIMRSGKEILNLLKPIGNATKRHEHGSCVVVHFCTATSLECSRVAPVINLLPHLFPTLPVAYIDAYEFTPFNAEFGIVSLPTLMIFHQGRPLIKYDTSWTDSEKRAFGRFIMRHTNVKTVDPKSIDPQILQRTLTEPLSNTPEVQTDYYLGMAWAFILLCLGNYVRGTIFWKQLIAMVQRNWRESEETQMDVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWV4</accession>
    <name>A0A6I8UWV4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6903351</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cytochrome c oxidase subunit 6A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002132232.3">
        <property type="nucleotide sequence ID" value="XM_002132196.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWV4"/>
    <dbReference type="FunCoup" id="A0A6I8UWV4">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6903351"/>
    <dbReference type="InParanoid" id="A0A6I8UWV4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UWV4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.95.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001349">
        <property type="entry name" value="Cyt_c_oxidase_su6a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036418">
        <property type="entry name" value="Cyt_c_oxidase_su6a_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02046">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81411">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="28"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_002132232.3"/>
        </source>
    </evidence>
    <sequence length="241" mass="26595" checksum="1B8CC94F82B56EBD" modified="2020-08-12" version="1">MLKLSRFATGMQITGHIRNAVFAMRQMSKGTKPPEKGPPPKGAAPKDPPKSPEKKKQDETAIRGGSKCSPKNKSAGAPAPGAVKKSCDDLLNKKSGKKKKQKLTAIQGGGQCGQKRKESAHSGGGMKLWRSISLFAILPMVAILTLLVFSTHKEHEHPEFVYYPFMYKRTKTYYFKDGNRTLFHNSKQNALPPAGYEDEIDEGGIGQEPETEKDKKQRLSDFQKLLKDWKKHAGAESAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY09</accession>
    <name>A0A6I8UY09_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lipase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Lip1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133107.2">
        <property type="nucleotide sequence ID" value="XM_002133071.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY09"/>
    <dbReference type="FunCoup" id="A0A6I8UY09">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902579"/>
    <dbReference type="InParanoid" id="A0A6I8UY09"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UY09">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000057">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026054817" evidence="9">
        <location>
            <begin position="25"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="10">
        <location>
            <begin position="68"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002133107.2"/>
        </source>
    </evidence>
    <sequence length="441" mass="50795" checksum="5A9FEA98D9AD0DDD" modified="2020-08-12" version="1">MRQKLKLHLILLLGLCVFINRTHAQLIGGDEDEEEEEEEEEEESVEDETFEEKLQRKNIKQDSSLSVDKLIAKYGYEAEVHHVTTEDGYILTMHRIRSQGSQPFLLQHGLVDSSAGFVVMGPNVSLAYLLADHNYDVWLGNARGNRYSRNHTSLDPDESKFWDFSWHEIGMYDLPAMIDYVLKVTGFQRLHYAGHSQGCTSFFVMCSMRPTYNEKVISMQALAPAVYAKETEDHPYIRAISLYFNSLVGSSIREMFNGEFRFLCRMTEETERLCIEAVFGIVGRNWNEFNRKMFPVILGHYPAGVAAKQVKHFIQIIKSGRFAPYSYSSNKNMLLYRDHQPPRYNLSLVTVPTFVYYSTNDLLCHPRDVESMCDDLGNVTGKYLVPVKEFNHMDFLWAIDVRKMLYQRMLQVLGKLPEDPQGEGRAVRVSRGARGRGLHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UY90</accession>
    <name>A0A6I8UY90_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein t-Grip91</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">t-Grip91</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002133185.3">
        <property type="nucleotide sequence ID" value="XM_002133149.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UY90"/>
    <dbReference type="FunCoup" id="A0A6I8UY90">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6902669"/>
    <dbReference type="InParanoid" id="A0A6I8UY90"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="7">
        <location>
            <begin position="529"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="6">
        <location>
            <begin position="820"/>
            <end position="1120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002133185.3"/>
        </source>
    </evidence>
    <sequence length="1146" mass="133145" checksum="F43FBFB4E3E94C56" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0D2</accession>
    <name>A0A6I8V0D2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor SOX-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Sox21b</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134931.3">
        <property type="nucleotide sequence ID" value="XM_002134895.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0D2"/>
    <dbReference type="FunCoup" id="A0A6I8V0D2">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900547"/>
    <dbReference type="InParanoid" id="A0A6I8V0D2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244926">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF322">
        <property type="entry name" value="SOX21B, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="251"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="251"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="23"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="455"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="62"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="119"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="475"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002134931.3"/>
        </source>
    </evidence>
    <sequence length="588" mass="62163" checksum="8BDAD5CE640E110F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0K3</accession>
    <name>A0A6I8V0K3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BTB/POZ domain-containing protein 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134930.2">
        <property type="nucleotide sequence ID" value="XM_002134894.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0K3"/>
    <dbReference type="KEGG" id="dpo:6900546"/>
    <dbReference type="InParanoid" id="A0A6I8V0K3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0K3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048512">
        <property type="term" value="P:circadian behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000005">
        <property type="entry name" value="BTB/POZ domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052407">
        <property type="entry name" value="BTB_POZ_domain_cont_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134930.2"/>
        </source>
    </evidence>
    <sequence length="369" mass="42271" checksum="FC8B53F5ADBDDAEB" modified="2020-08-12" version="1">MNHATSSSSVDIILGDLVLEDMDRFCMNELFSDVSFLVGDQSLPAHSVILAARSEYFCAMLYGGMSVLNERQIRLESVPLEAFKVILRYLYSGKLHISTLDASCEVLGLANMYCLLEVESALVKHLLENMTVSNVWMILDMGHTYNLSQLANGCLKYIDNNGDQMLEHDSFQMLSKQSLEEVLRRDSFKVREVKIFKAVCKWNRHNPNEDIKSVMSLVRLSLMSADELIQVVRPSKIVEPEKIADAIEKALLPKNQNLPYRCTNYPDKDVKFLNTSTEGIFIDLSWWWLINCMCVLPSKPFDTWNCAMEVSCDMTHWDRAGTGTFQLAIHCEYITFTTRPVRYIRMVDTESGRTDLINYVLIQVKLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0Z0</accession>
    <name>A0A6I8V0Z0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6900864</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="10"/>
    <dbReference type="RefSeq" id="XP_002135221.2">
        <property type="nucleotide sequence ID" value="XM_002135185.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0Z0"/>
    <dbReference type="KEGG" id="dpo:6900864"/>
    <dbReference type="InParanoid" id="A0A6I8V0Z0"/>
    <dbReference type="UniPathway" id="UPA00753">
        <property type="reaction ID" value="UER00739"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0Z0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="104"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002135221.2"/>
        </source>
    </evidence>
    <sequence length="389" mass="43498" checksum="2A53E24B26AB7FB7" modified="2020-08-12" version="1">MDQIIEPNASLLSGSGSGRSTPSFEGFQMDDPDTKDPEPAVAFETQYPAMSLYDVNLDKYPFCQPCPFATDAKALEELVACDYTQRISYEMARSGQATRRVRVYADGIYDLFHQGHARQLLQAKNIFPNVYLIVGVCNNELTLKMKGRTVMNGFERYEAVRHCRYVDEIVANAPWTLTDEFLNEHKIDFVAHDDIPYGGAGGVDDIYAHLKARGMFVATERTPGVSTSDIVARIVKDYDLFVRRNLARGYTAKELNVSFLSEKKFRFQNKMDELKDRGNMVRCGLLARWEAKSRDLIEAFLQAFGRERLCSIWRESKGKLMSIWGSAASLEAGAIDISDSEDEYEDALGEVAHVVAESDSGLETARWHDTVAPNLSRSSSSPDVGTGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V236</accession>
    <name>A0A6I8V236_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6896973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137158.2">
        <property type="nucleotide sequence ID" value="XM_002137122.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V236"/>
    <dbReference type="KEGG" id="dpo:6896973"/>
    <dbReference type="InParanoid" id="A0A6I8V236"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026348411" evidence="1">
        <location>
            <begin position="27"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137158.2"/>
        </source>
    </evidence>
    <sequence length="114" mass="12307" checksum="51690A875B1A974E" modified="2020-08-12" version="1">MNATGLLLLMGLALSLQLILMGPTSCAPVSDDLESPSSIKEIGGLAAFAYTRKKVAKTPPKTNLIMPPSKNMTRRQRMEYADDCEPLGMVCKFAEQCCTKACMVATRRCAFVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2N3</accession>
    <name>A0A6I8V2N3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137331.1">
        <property type="nucleotide sequence ID" value="XM_002137295.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2N3"/>
    <dbReference type="GeneID" id="6897151"/>
    <dbReference type="KEGG" id="dpo:6897151"/>
    <dbReference type="InParanoid" id="A0A6I8V2N3"/>
    <dbReference type="OMA" id="QTFTHHF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0248515">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20987">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20987:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026346852" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10371" checksum="0241A37DF19A28B9" modified="2020-08-12" version="1">MNSVFVALCAVFLAAMLMGGVNADAGRSACQDESELGQTYTHHFDAAKYWLCETLGVPATEVDCPSGLAYMHLLKECIPWASYIWKKPEMPPTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V301</accession>
    <name>A0A6I8V301_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC6897365</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137518.2">
        <property type="nucleotide sequence ID" value="XM_002137482.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V301"/>
    <dbReference type="KEGG" id="dpo:6897365"/>
    <dbReference type="InParanoid" id="A0A6I8V301"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030865">
        <property type="term" value="P:cortical cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21505">
        <property type="entry name" value="PPP2R3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041534">
        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039865">
        <property type="entry name" value="PPP2R3C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="337"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002137518.2"/>
        </source>
    </evidence>
    <sequence length="439" mass="51198" checksum="01D85FFA49EAADFE" modified="2020-08-12" version="1">MELDPDLIEQFSNLMVSTRPISAEELARFDENAPLRQRNEIDHPSYKTIPKFFEAPLPPGDTIRQNLRKEAHSLYLQQKSQELLDSNELDELWSMLLKHVTQMTATGQQLIDFDAYLQLQGAVNAKIRNYLTARLFGTLLVLSTHLDGMVDIVSVFNYIMRKVWLKQARIGLSFYDEMGRGYLRESDMESYIIDLIPNMAQIRDCMQPSFQSFYVCTVLKRFFFFLDAQHRGRICIRAMLESGMVTDLLDLREEADPEDPQDKNCFSMPAVLAVYRNYLSLDKDHNGMLSQKELADYGSGTLTTIFLERVFEECRTYQGEMDYKTYLDFDMAMNNRRSPQALQYLFRILDVDHKGYLTVQTLHFFFKGIQEAFQSHNEVPVNFEDINNEIFDMVKPNDPCKITLQDLIKSGKAETVVSILIEFHKFWAYETREENPQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V381</accession>
    <name>A0A6I8V381_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dynein regulatory complex protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137851.3">
        <property type="nucleotide sequence ID" value="XM_002137815.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V381"/>
    <dbReference type="FunCoup" id="A0A6I8V381">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897746"/>
    <dbReference type="InParanoid" id="A0A6I8V381"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000174">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052267">
        <property type="entry name" value="N-DRC_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690">
        <property type="entry name" value="IQ MOTIF CONTAINING WITH AAA DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690:SF0">
        <property type="entry name" value="IQ MOTIF CONTAINING WITH AAA DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="2">
        <location>
            <begin position="570"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137851.3"/>
        </source>
    </evidence>
    <sequence length="831" mass="95875" checksum="FEFABB509C3DD2AF" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3Z7</accession>
    <name>A0A6I8V3Z7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138339.3">
        <property type="nucleotide sequence ID" value="XM_002138303.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3Z7"/>
    <dbReference type="KEGG" id="dpo:6898272"/>
    <dbReference type="InParanoid" id="A0A6I8V3Z7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002138339.3"/>
        </source>
    </evidence>
    <sequence length="159" mass="18288" checksum="981D538D7F75CAF5" modified="2020-08-12" version="1">MTRDQSKFDVLQSNTHHLIVAMNQLTLVFCLLLWSEVDGAFRDFVVEPEIDSEDSELENNFQDREGALDENLLVSFQSVERDGIVDTQLLLKALMQHAQRLGLSLDELASMSYGEAEDEEAANQLESMSRNQDMMGCNSRDQEMFEYADRPTWRDVIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4H2</accession>
    <name>A0A6I8V4H2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6898674</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138679.1">
        <property type="nucleotide sequence ID" value="XM_002138643.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4H2"/>
    <dbReference type="FunCoup" id="A0A6I8V4H2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="6898674"/>
    <dbReference type="KEGG" id="dpo:6898674"/>
    <dbReference type="InParanoid" id="A0A6I8V4H2"/>
    <dbReference type="OMA" id="ECIGMEI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245661">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002138679.1"/>
        </source>
    </evidence>
    <sequence length="52" mass="5831" checksum="166AEB9988108D63" modified="2020-08-12" version="1">MVKSVNPLSFVRGIYNNEFQWMLVKSYALFFLGVRVAKELVGLELMPAVGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4H3</accession>
    <name>A0A6I8V4H3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138599.2">
        <property type="nucleotide sequence ID" value="XM_002138563.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4H3"/>
    <dbReference type="KEGG" id="dpo:6898579"/>
    <dbReference type="InParanoid" id="A0A6I8V4H3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026267314" evidence="1">
        <location>
            <begin position="23"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138599.2"/>
        </source>
    </evidence>
    <sequence length="165" mass="18223" checksum="4D7A31604CAF860E" modified="2020-08-12" version="1">MGLGRTWIILGLIGSIACLGNAEPRLLGSEEDASLEDDNLSFLNSKSQKFASSATTIIENDHTLIYGSGDNNKITYNGHRFVVDHGIIKLFEGSKTYTFRPLGANVEKRQTIDFNGRPATVQLSNGNVFVYLPDGTVMGRIGNSYFVGDRYAVENRDRMIRNSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4W2</accession>
    <name>A0A6I8V4W2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6898740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138727.2">
        <property type="nucleotide sequence ID" value="XM_002138691.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4W2"/>
    <dbReference type="KEGG" id="dpo:6898740"/>
    <dbReference type="InParanoid" id="A0A6I8V4W2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002138727.2"/>
        </source>
    </evidence>
    <sequence length="54" mass="5488" checksum="EF1A965C803038E5" modified="2020-08-12" version="1">MPSKKKDSAKSSKKGAQEKAPSTDPALATSTASAPETSPVKQSKGSKKGKGTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4Z9</accession>
    <name>A0A6I8V4Z9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6899106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002139023.2">
        <property type="nucleotide sequence ID" value="XM_002138987.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4Z9"/>
    <dbReference type="KEGG" id="dpo:6899106"/>
    <dbReference type="InParanoid" id="A0A6I8V4Z9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002139023.2"/>
        </source>
    </evidence>
    <sequence length="278" mass="32409" checksum="EF586BE4FC0B613D" modified="2020-08-12" version="1">MVGHLPPIEVLMGIVLLFGSAKATFNWDLYGTLTGLVLRHLNGSISDFDWWTLRMSHALEPEQQQRLAWNMPWTQALRQGRSLLELQEQLRPSNPLKLQLWMGFYWHLRRSSRLDGRLTFDFARTLRRLQLQQQPQPELWSPQLQNMWQSLPRSMRLILHSRWLCLQHEREMLYAVGGLRLELGANSNCSMWQVQELSEQRGHWLGLFNVCDKNSRWFISMQEHDAATHLLHSVPSHTARKLCVLEGLGYLGDAVSPLSDCHWKLNDCSHLPQILFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V505</accession>
    <name>A0A6I8V505_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chitinase-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6898990</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138950.1">
        <property type="nucleotide sequence ID" value="XM_002138914.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V505"/>
    <dbReference type="FunCoup" id="A0A6I8V505">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="GeneID" id="6898990"/>
    <dbReference type="KEGG" id="dpo:6898990"/>
    <dbReference type="InParanoid" id="A0A6I8V505"/>
    <dbReference type="OMA" id="RDSTRSM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245507">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026042582" evidence="5">
        <location>
            <begin position="21"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="7">
        <location>
            <begin position="23"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="6">
        <location>
            <begin position="389"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002138950.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="53280" checksum="2EF0EA7CB855F246" modified="2020-08-12" version="1">MWSLNCAFVLMIFMIYGCAAKDFKIFCQYDMGSFYYKNSSKFFSWNIDLRLCTHLVYGTGVGVDGETGDVTITDKYLLVENDILNVAHRLKRDNVKKVMFTIGGWLEESSDFSKMVADARKRDRFFTSLVRFMYGWGFDGVQIDWRYPTQRGGKPEDRKNFILLLEELKIICQEHQFILMVAVLGRTDKSTLEWYDIPKLVQYVHYINLMHHDNRDPYKMHLSYNAPLSGKNGVIESVKLWKKLSKAPEKLILNIPLFVRSYIMEKHKTTVGSPCKGPGLKTALTRKSGFMTYNEWCVQADTWTQKFDEQAQVPYAYRDDHWISFENNRSITEKLKLLKSQNLGGAISSTIDADDFSGKCGKKHALERVILETLDPQTVLVDMDAPTEPPPCLKDGVFRDINDCQLYYVCSAGHRSDYECRWQEFFDEAKSECRPMKEVKCEVLEAALKARELRDSTRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V510</accession>
    <name>A0A6I8V510_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">General transcription and DNA repair factor IIH helicase subunit XPD</fullName>
            <ecNumber evidence="18">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">CXPD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">DNA 5'-3' helicase XPD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">DNA excision repair protein ERCC-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">DNA repair protein complementing XP-D cells</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Xeroderma pigmentosum group D-complementing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">Xpd</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="27">MV-25-SWS-2005</strain>
            <tissue evidence="27">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="18"/>
    <dbReference type="RefSeq" id="XP_002138963.1">
        <property type="nucleotide sequence ID" value="XM_002138927.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V510"/>
    <dbReference type="FunCoup" id="A0A6I8V510">
        <property type="interactions" value="1826"/>
    </dbReference>
    <dbReference type="GeneID" id="6899003"/>
    <dbReference type="KEGG" id="dpo:6899003"/>
    <dbReference type="InParanoid" id="A0A6I8V510"/>
    <dbReference type="OMA" id="WQTMGIL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246483">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035315">
        <property type="term" value="P:hair cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045951">
        <property type="term" value="P:positive regulation of mitotic recombination"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA repair helicase RAD3, putative"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative DNA repair helicase RAD3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TFIIH basal transcription factor complex helicase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ATP-dep_Helicase_C"/>
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        <property type="entry name" value="DinG/Rad3-like"/>
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    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
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        <property type="entry name" value="HBB"/>
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    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
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        <property type="entry name" value="Helicase-like_DEXD_c2"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
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    <dbReference type="InterPro" id="IPR001945">
        <property type="entry name" value="RAD3/XPD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="XRODRMPGMNTD"/>
    </dbReference>
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        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 27" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="25">
        <location>
            <begin position="7"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00081188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_002138963.1"/>
        </source>
    </evidence>
    <sequence length="769" mass="87976" checksum="56411FF32D194B02" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5Q8</accession>
    <name>A0A6I8V5Q8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Unconventional myosin-Ib isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">Myo95E</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137493.2">
        <property type="nucleotide sequence ID" value="XM_002137457.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003736597.3">
        <property type="nucleotide sequence ID" value="XM_003736549.3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897340"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF802">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IB ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="8">
        <location>
            <begin position="5"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="9">
        <location>
            <begin position="1122"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="313"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="371"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="6">
        <location>
            <begin position="832"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="190"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="384"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_002137493.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_003736597.3"/>
        </source>
    </evidence>
    <sequence length="1307" mass="149819" checksum="6F554845BA737C79" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5Z9</accession>
    <name>A0A6I8V5Z9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Pneumococcal serine-rich repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">dpr17</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736769.3">
        <property type="nucleotide sequence ID" value="XM_003736721.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236476.1">
        <property type="nucleotide sequence ID" value="XM_033380585.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236477.1">
        <property type="nucleotide sequence ID" value="XM_033380586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5Z9"/>
    <dbReference type="KEGG" id="dpo:4802598"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032589">
        <property type="term" value="C:neuron projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001405">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001026">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037448">
        <property type="entry name" value="Zig-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279:SF21">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE 11, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23279">
        <property type="entry name" value="DEFECTIVE PROBOSCIS EXTENSION RESPONSE DPR -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="472"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="561"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_003736769.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033236476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033236477.1"/>
        </source>
    </evidence>
    <sequence length="721" mass="76508" checksum="68B264E624491CC6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V676</accession>
    <name>A0A6I8V676_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Usp20-33</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="RefSeq" id="XP_002138937.2">
        <property type="nucleotide sequence ID" value="XM_002138901.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V676"/>
    <dbReference type="FunCoup" id="A0A6I8V676">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898977"/>
    <dbReference type="InParanoid" id="A0A6I8V676"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000007">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF86">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="82"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="5">
        <location>
            <begin position="537"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="5">
        <location>
            <begin position="645"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="196"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="268"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="762"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="199"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="762"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="779"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="804"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="819"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="848"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="887"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="942"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002138937.2"/>
        </source>
    </evidence>
    <sequence length="975" mass="109859" checksum="7DF1A90E3F6F9542" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6Y0</accession>
    <name>A0A6I8V6Y0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hepatoma-derived growth factor-related protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4801650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736559.2">
        <property type="nucleotide sequence ID" value="XM_003736511.3"/>
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    <dbReference type="KEGG" id="dpo:4801650"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="1">
        <location>
            <begin position="11"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_003736559.2"/>
        </source>
    </evidence>
    <sequence length="162" mass="18045" checksum="31F6AB4D834170A3" modified="2020-08-12" version="1">MGTRKKAPFTAGQFVFGKVRGFRAWPGRLINELGSAYNVYFYGTCDYAKVPKNKIFDFAKTCKRYGKVHDRNSKVCRSFQEAMSQIQKAVSQPSLDFGYFTMVAHLEQNCGESDSDSDSDSGTLDLSGAELKGKLVPFRHIVADSDTDTEMSSEPKKTPNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VA21</accession>
    <name>A0A6I8VA21_DROPS</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26533818</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015036906.2">
        <property type="nucleotide sequence ID" value="XM_015181420.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VA21"/>
    <dbReference type="KEGG" id="dpo:26533818"/>
    <dbReference type="InParanoid" id="A0A6I8VA21"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015036906.2"/>
        </source>
    </evidence>
    <sequence length="252" mass="28534" checksum="8874527CBFA94D65" modified="2020-08-12" version="1">MSDPHRGLRGHSKLRLYLWIYGTALVFIFISILLIISFGFINNEPDKSCCPAAFSFFSLGLLSMFFYMNMMFLRRKFPINWIMSCSMAVVFGLGTASMLCMQTAGHVILLALEVIVMMGLLLLLGYWLPPKCHPLLYIALTSFILAVIAFFLCKIISDHTDENHDKVAVWIARFVLWTAICPLLLFQSQVINGYWGNESPYLDIPLCSILLLIDFLACYAFLDAVDDIEIAYDRLFRSETVGLIARVGRSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VC33</accession>
    <name>A0A6I8VC33_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Chloride channel protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ClC-a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015038128.2">
        <property type="nucleotide sequence ID" value="XM_015182642.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VC33"/>
    <dbReference type="FunCoup" id="A0A6I8VC33">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VC33"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VC33">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03683">
        <property type="entry name" value="ClC_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000003">
        <property type="entry name" value="Chloride channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000026">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000032">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF10">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="11" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="320"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="578"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="607"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="67"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="854"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="982"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1189"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="860"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1005"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1191"/>
            <end position="1216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015038128.2"/>
        </source>
    </evidence>
    <sequence length="1216" mass="136422" checksum="877EC8E89E62C0D8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VCD3</accession>
    <name>A0A6I8VCD3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formin-binding protein 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Tm1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015038280.2">
        <property type="nucleotide sequence ID" value="XM_015182794.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VCD3"/>
    <dbReference type="InParanoid" id="A0A6I8VCD3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="entry name" value="TROPOMYOSIN"/>
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        <property type="entry name" value="Tropomyosin"/>
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        <property type="entry name" value="TROPOMYOSIN"/>
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            <end position="82"/>
        </location>
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            <begin position="232"/>
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        </location>
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            <begin position="322"/>
            <end position="489"/>
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            <begin position="605"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00009036"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015038280.2"/>
        </source>
    </evidence>
    <sequence length="1010" mass="108238" checksum="DBB3C17D0EA03FC0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name evidence="4 5 6" type="primary">LOC6897026</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
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                <consortium name="RefSeq"/>
            </authorList>
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            <strain evidence="4 5 6">MV-25-SWS-2005</strain>
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        </source>
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            <begin position="1"/>
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        <source>
            <dbReference type="RefSeq" id="XP_033232595.1"/>
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</entry>
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        <name evidence="3" type="primary">LOC6898228</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <lineage>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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                <consortium name="RefSeq"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">MV2-25</strain>
        </source>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
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        </location>
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        </location>
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        </location>
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            <begin position="414"/>
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        </location>
    </feature>
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            <end position="625"/>
        </location>
    </feature>
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        <location>
            <begin position="657"/>
            <end position="667"/>
        </location>
    </feature>
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            <end position="743"/>
        </location>
    </feature>
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            <begin position="752"/>
            <end position="769"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015039328.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="965" mass="108004" checksum="2B63447ABFE69279" modified="2020-08-12" version="1"> </sequence>
</entry>
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    <accession>A0A6I8VEV8</accession>
    <name>A0A6I8VEV8_DROPS</name>
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        <recommendedName>
            <fullName evidence="14">FXNA-like protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4804261</name>
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    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family.</text>
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    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="dpo:4804261"/>
    <dbReference type="InParanoid" id="A0A6I8VEV8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000008">
        <property type="entry name" value="Endoplasmic reticulum metallopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053973">
        <property type="entry name" value="ERMP1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF22">
        <property type="entry name" value="ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22248">
        <property type="entry name" value="ERMP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="450"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="539"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="584"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="16">
        <location>
            <begin position="141"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum metallopeptidase 1-like C-terminal" evidence="17">
        <location>
            <begin position="638"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015039777.2"/>
        </source>
    </evidence>
    <sequence length="866" mass="98368" checksum="DA039C554ED93856" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFD3</accession>
    <name>A0A6I8VFD3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26532023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040092.1">
        <property type="nucleotide sequence ID" value="XM_015184606.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFD3"/>
    <dbReference type="GeneID" id="26532023"/>
    <dbReference type="KEGG" id="dpo:26532023"/>
    <dbReference type="InParanoid" id="A0A6I8VFD3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271010">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015040092.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="19259" checksum="25392C439AF9B647" modified="2020-08-12" version="1">MRKSREPSNGSSGRAITWENGRERARLRMANVIVDRCSACCGGEQEREIEEQTADAAQALLSIDRTAFWHSLKLYVQVNRISDGQRAAPLESVRVSINSFVSQNATNQLKPSRPLVTSMESVIESLRQSKHRLGRTPTLLSVSVVEAARASAVQVKDVVVFEWIYRRIYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFE4</accession>
    <name>A0A6I8VFE4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13 14">NF-kappa-B essential modulator isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13 14" type="primary">key</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 14">MV-25-SWS-2005</strain>
            <strain evidence="11">MV2-25</strain>
            <tissue evidence="12 14">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015040121.2">
        <property type="nucleotide sequence ID" value="XM_015184635.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040122.2">
        <property type="nucleotide sequence ID" value="XM_015184636.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040123.2">
        <property type="nucleotide sequence ID" value="XM_015184637.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFE4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VFE4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09803">
        <property type="entry name" value="UBAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.990">
        <property type="entry name" value="Nemo cc2-lz domain - 1d5 darpin complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032419">
        <property type="entry name" value="CC2-LZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034735">
        <property type="entry name" value="NEMO_ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051301">
        <property type="entry name" value="Optineurin/NFkB_EssMod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31553">
        <property type="entry name" value="NF-KAPPA-B ESSENTIAL MODULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31553:SF1">
        <property type="entry name" value="NF-KAPPA-B ESSENTIAL MODULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16516">
        <property type="entry name" value="CC2-LZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51801">
        <property type="entry name" value="ZF_CCHC_NOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC NOA-type" evidence="10">
        <location>
            <begin position="417"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="378"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="145"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015040121.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015040122.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_015040123.2"/>
        </source>
    </evidence>
    <sequence length="449" mass="51256" checksum="3F736C276B36C187" modified="2020-08-12" version="1">MTSASDVGAMSEEESFVILGSSPYPSLCTDGSVLIADAMNTGDGRVTVAQEIDVSDEPAAVSSSGPMSTSVDSDRHKSLAASFIMGEVKSDVLKNSVYSQFPSLCSLQASAEDVVKLQHMMAEYVSLKKTLDMVNRTMQDYYKLTQQWRQEADHKEQQYKQQLKECQEQVEKLRAENQKLKAELETKLEQIELVQDIRQKEQDELRQSVSEKTSLINNMRVEIDKLQQIKLNSFEFVPKDSEKAAEIEVSNPLNYVQREEHDRQIRELQRELSKILAENLEVNDMKKVYIQEIDCLKVNLTSAQELMDKMQRDINQLKDRDIQKQEQIEHLQTQNEIYRRDFEMERADREKNAGEKEQYLLDLRALQRRNQELIEALAESHKSPKSGSPSRSSSGISSLREDQRPVRVLDPTGAAVRPSDSTLRCPICSKSFNSLSVLQSHVNDCLDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VFQ6</accession>
    <name>A0A6I8VFQ6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26532631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015040232.2">
        <property type="nucleotide sequence ID" value="XM_015184746.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VFQ6"/>
    <dbReference type="KEGG" id="dpo:26532631"/>
    <dbReference type="InParanoid" id="A0A6I8VFQ6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271618">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015040232.2"/>
        </source>
    </evidence>
    <sequence length="112" mass="12240" checksum="4F06DDF8D9CBCCFD" modified="2020-08-12" version="1">MSASVDSPFSNATTAYGPIERLIHYRWYTQLQRESIFKVRHLHRHAATPISSACRVGAVQRATSCAPPSGQVSSDYPKCLKNHSDPCIHSNLMSTRELAAVAAAESLCRAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VHV2</accession>
    <name>A0A6I8VHV2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC26532389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041460.2">
        <property type="nucleotide sequence ID" value="XM_015185974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VHV2"/>
    <dbReference type="KEGG" id="dpo:26532389"/>
    <dbReference type="InParanoid" id="A0A6I8VHV2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF67">
        <property type="entry name" value="COREPRESSOR OF PANGOLIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="1">
        <location>
            <begin position="5"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_015041460.2"/>
        </source>
    </evidence>
    <sequence length="206" mass="23689" checksum="AF0A598154DA4001" modified="2020-08-12" version="1">MDCEKLLSEIAVSPVVWNKHHTNYSNRLILEKTWQAIADKMGKPAEQCKKKFKYLKDEYRKAQRNVSKLASGAAAAESHVSKFKYFNLMHFLKDDFGGDACGLMDEKSMAFSDNTTQIMTVRSAKDDFEKAGPSKQRQEDMDNEIIEIEKEKEIEKIENNGNNKESDDLLFFKSLIPLMPSSLSASQKLRMRTDFLNVVLKYLDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNJ5</accession>
    <name>A0A6I8VNJ5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fatty-acid amide hydrolase 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC6898018</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033232650.1">
        <property type="nucleotide sequence ID" value="XM_033376759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNJ5"/>
    <dbReference type="FunCoup" id="A0A6I8VNJ5">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898018"/>
    <dbReference type="InParanoid" id="A0A6I8VNJ5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VNJ5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF2">
        <property type="entry name" value="IP13792P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="87"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="535"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232650.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="63634" checksum="553BB090736EE89E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNU5</accession>
    <name>A0A6I8VNU5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein suppressor of variegation 3-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Su(var)3-7</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033232735.1">
        <property type="nucleotide sequence ID" value="XM_033376844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNU5"/>
    <dbReference type="FunCoup" id="A0A6I8VNU5">
        <property type="interactions" value="417"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897163"/>
    <dbReference type="InParanoid" id="A0A6I8VNU5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VNU5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004210">
        <property type="entry name" value="BESS_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02944">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51031">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BESS" evidence="3">
        <location>
            <begin position="914"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="468"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="593"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="685"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="797"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="976"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1147"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="501"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="613"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="685"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="977"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1023"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1178"/>
            <end position="1191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033232735.1"/>
        </source>
    </evidence>
    <sequence length="1191" mass="133417" checksum="8E758898BF3EE509" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPW8</accession>
    <name>A0A6I8VPW8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28 29" type="primary">InR</name>
    </gene>
    <organism evidence="27 29">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="28 29" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="28 29">MV-25-SWS-2005</strain>
            <strain evidence="27">MV2-25</strain>
            <tissue evidence="28 29">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
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            <dbReference type="PROSITE" id="PS51379"/>
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            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_002137842.4"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033233087.1"/>
        </source>
    </evidence>
    <sequence length="2290" mass="254631" checksum="3B9FE1D1268353A4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQM6</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
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        <name evidence="2" type="primary">LOC26532927</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233380.1">
        <property type="nucleotide sequence ID" value="XM_033377489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQM6"/>
    <dbReference type="KEGG" id="dpo:26532927"/>
    <dbReference type="InParanoid" id="A0A6I8VQM6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233380.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="19274" checksum="8265CB84EC16AA99" modified="2020-08-12" version="1">MKYKLFYKPGTDTFGNADRPGGTYIPLLLSRRGQMCCDEQLVYKMTNIECLGNPERVQNFSHYLKAVNMDCYITVPLRNPVVSWKDTTSGPKLIDISAMFVYFRFQGHLYGRNGFLDPSLVPPFTIGFYQVSGVVVDRNAAASEYVGTVKFFLQAMEAVKSKKRPTSKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VTD5</accession>
    <name>A0A6I8VTD5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234332.1">
        <property type="nucleotide sequence ID" value="XM_033378441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VTD5"/>
    <dbReference type="KEGG" id="dpo:6898029"/>
    <dbReference type="InParanoid" id="A0A6I8VTD5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234332.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="57382" checksum="F77D8E861A955CE7" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VW20</accession>
    <name>A0A6I8VW20_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">ATPase family AAA domain-containing protein 1-B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">nmd</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10">MV-25-SWS-2005</strain>
            <tissue evidence="9 10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235284.1">
        <property type="nucleotide sequence ID" value="XM_033379393.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235285.1">
        <property type="nucleotide sequence ID" value="XM_033379394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VW20"/>
    <dbReference type="KEGG" id="dpo:117183914"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140570">
        <property type="term" value="P:extraction of mislocalized protein from mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19520">
        <property type="entry name" value="RecA-like_ATAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000538">
        <property type="entry name" value="ATPase family AAA domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF3">
        <property type="entry name" value="FI08533P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="131"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033235284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033235285.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="41668" checksum="FF08A423E35BC951" modified="2020-08-12" version="1">MDHFSFGGADLTRGQIFQVIIRLSVASMVTYYSVKWMMNQLDPTSKNKKKAKGLAEEQLKRLSENDGLKINAHDFNDYELMIASHLVVPADITVGWSDIAGLNLIVQELRESVVLPVQHKDLFKSSKLWQAPKGVLLHGPPGCGKTLIAKATAKEAGMRFINLDVAILTDKWYGESQKLTSAVFSLAAKIEPCIIFIDEIDSFLRARNLNDHEATAMMKTQFMMLWDGLSTNTNSTVIVMGATNRPQDLDKAIVRRMPAQFHIGLPSETQRTEILKLILESEEVSPDVDLNRLSKLTNGFSGSDLREMCRNASVFRMRQLIEVKNNSGSSISVIDKTNLKITMDDLLSSHLKIKESKMHTGSLFLENRIDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VW96</accession>
    <name>A0A6I8VW96_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta-gliadin clone PW1215-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235351.1">
        <property type="nucleotide sequence ID" value="XM_033379460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VW96"/>
    <dbReference type="KEGG" id="dpo:6903031"/>
    <dbReference type="InParanoid" id="A0A6I8VW96"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VW96">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033235351.1"/>
        </source>
    </evidence>
    <sequence length="174" mass="19818" checksum="A44FB9287F3EE3BF" modified="2020-08-12" version="1">MIHKPQPQPLPLQQPLPLPLPQPRPKPKPLPLSQVPMRPFIPPLTHTGNPLHPQPQPHSSGQWLYSSSSLAHTTSFSQPRSRRELYPLRHQPQSYPQARPHSLRHTHSYTGPSSEYHRHPVQETSFALPPRAARGDPFRPVPSLSNVEAVTHAIRNKIILEEDEEDILGSDRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VWE4</accession>
    <name>A0A6I8VWE4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">Dyrk2</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17">MV-25-SWS-2005</strain>
            <tissue evidence="16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001356205.4">
        <property type="nucleotide sequence ID" value="XM_001356169.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033235273.1">
        <property type="nucleotide sequence ID" value="XM_033379382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VWE4"/>
    <dbReference type="KEGG" id="dpo:4816475"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    <sequence length="730" mass="80597" checksum="46ABEEFB1799228F" modified="2020-08-12" version="1"> </sequence>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236530.1"/>
        </source>
    </evidence>
    <sequence length="1035" mass="113672" checksum="D90EF3001371F5AC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZR0</accession>
    <name>A0A6I8VZR0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Double-strand-break repair protein rad21-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">vtd</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the rad21 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033236580.1">
        <property type="nucleotide sequence ID" value="XM_033380689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZR0"/>
    <dbReference type="FunCoup" id="A0A6I8VZR0">
        <property type="interactions" value="2276"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:26532716"/>
    <dbReference type="InParanoid" id="A0A6I8VZR0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21792">
        <property type="entry name" value="Rad21_Rec8_M_NXP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.580:FF:000006">
        <property type="entry name" value="Cohesin subunit rad21, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049589">
        <property type="entry name" value="NXP1_M-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF69">
        <property type="entry name" value="FI11703P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="7">
        <location>
            <begin position="650"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="195"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="528"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="585"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="531"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033236580.1"/>
        </source>
    </evidence>
    <sequence length="709" mass="78802" checksum="028BFF409687438C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W205</accession>
    <accession>B5DPH1</accession>
    <name>A0A6I8W205_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Basic-leucine zipper transcription factor A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">Ccn</name>
    </gene>
    <organism evidence="8 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 10 11">MV-25-SWS-2005</strain>
            <tissue evidence="9 10 11">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134891.2">
        <property type="nucleotide sequence ID" value="XM_002134855.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237390.1">
        <property type="nucleotide sequence ID" value="XM_033381499.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237391.1">
        <property type="nucleotide sequence ID" value="XM_033381500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W205"/>
    <dbReference type="SMR" id="A0A6I8W205"/>
    <dbReference type="GeneID" id="6900502"/>
    <dbReference type="KEGG" id="dpo:6900502"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244950">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF17">
        <property type="entry name" value="CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634593" evidence="5">
        <location>
            <begin position="22"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="7">
        <location>
            <begin position="39"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="6">
        <location>
            <begin position="233"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="172"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="302"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="390"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="188"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="391"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="504"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002134891.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033237390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033237391.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="58916" checksum="8F832D23ADAC2D8A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W206</accession>
    <name>A0A6I8W206_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BTB/POZ domain-containing protein 9-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236719.1">
        <property type="nucleotide sequence ID" value="XM_033380828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W206"/>
    <dbReference type="KEGG" id="dpo:6903477"/>
    <dbReference type="InParanoid" id="A0A6I8W206"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W206">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:adult locomotory behavior"/>
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        <property type="term" value="P:circadian behavior"/>
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    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
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        <property type="entry name" value="BTB_POZ_domain_cont_9"/>
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        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
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        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BTB"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033236719.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="42928" checksum="1D4DD09167A323ED" modified="2020-08-12" version="1">MSSQANKDTSSSPNSTNEYIIDYSGSLVADMASLCMNEPYSDVEFLVENQRLPGHRLVLATRSEYFRALLYGGLAESNQREVPLEVPLEAFKLILGYLYSGKMPLSTLDVDTIIDVLDLAHLYGLQAVESGIGKYLQQNLSVSNVCTILDVARRKNLNQRAEECLNFIDNNGSDIVKHDSFAQLPKESLEELLRRDTFAALEIDIFRAVCKWRDNHPSEDFKTVAALVRLPLLTVQQLVQEVRPSGLFEPDQIFNAIGQVDTGKNLTYRVVVLPEKNVASEKHLARRFQDEQNHIVFELRNLFAINFIELIFQYGSYSIRFEVHVSCDQTHWNCVGSKSVYETATHYLTFKQRPVRYIRIRTAPTTNVLSLTAMYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W3M0</accession>
    <name>A0A6I8W3M0_DROPS</name>
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        <submittedName>
            <fullName evidence="3 4 5">Uncharacterized protein Aven</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5" type="primary">Aven</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Pterygota</taxon>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4 5">MV-25-SWS-2005</strain>
            <tissue evidence="3 4 5">Whole body</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_001355343.4"/>
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        <property type="nucleotide sequence ID" value="XM_033382049.1"/>
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        <property type="nucleotide sequence ID" value="XM_033382050.1"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Aven"/>
    </dbReference>
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        <property type="entry name" value="CELL DEATH REGULATOR AVEN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CELL DEATH REGULATOR AVEN"/>
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        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
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        <location>
            <begin position="217"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
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            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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            <dbReference type="RefSeq" id="XP_001355379.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033237940.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033237941.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="33903" checksum="4D94B2B17E88376A" modified="2020-08-12" version="1">MSGKDDRSKEKRRNMKQYTHHKPQPQHQQQRRGTTTTTSSSSPQTTAVRSIVDVADSSDEPRFARKGNWASGGGSRGSTLAPQMNRDLLDVLPSDTDDVDHLGIDTGAPIDENARAQLRAGDYKQLTQFPNLGGGHFTFGSEKEWTSIAEGQTQLHTKAASGYFTLNLVRLNAGLQTIPFYKRMDYPASMFTRQQIVAQTEAAERAETLYQQRVLREANGSASKSRAASAKSAKVPAKPQPVVTAPPEGALADDLEELLAMTNIQPDPNTIVPDPIAMPMPMPMPMVQSATPTSAASKNDVENWLDNVLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4T5</accession>
    <name>A0A6I8W4T5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LOW QUALITY PROTEIN: uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4812731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238338.1">
        <property type="nucleotide sequence ID" value="XM_033382447.1"/>
    </dbReference>
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        <property type="interactions" value="76"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UstE-like"/>
    </dbReference>
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        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251:SF15">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE (DUF1295)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06966">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="S5A_REDUCTASE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238338.1"/>
        </source>
    </evidence>
    <sequence length="495" mass="55163" checksum="E56C138707926379" modified="2020-08-12" version="1">MAVHVLDYDHFAISAIVVGSLQLFFFLINSILHMDKLTDFAGGVNFIIISLLTFFLGQIDRPSKAYDSRQLMVTLFVCLWGARLSGYLLYRIIKLGRDKQFEDTRRNVIRYAVFWTFQAVWVYIVSLPVIIINSPRHSQPHAPKTMTTLDSTGTGMFIVGLLAETYADLQKFSFRQDPANQGKFCNDGLWSVSRHPNYFGEVIIWWGIFAISLNVISGHEWVAIASPIFTTLIILFLSGIPLRERSADEKYKDQLEYRKYKASTSPLIPVPPAVYVEVPSALKFILCCEFPIYDSMSIQKDLSPYSLSIARSHSHIXQSVAHSSSTQAGVGQVHTHSVAHSLHRGHCYGANGGDAESHHNCGGSCRNVHVKSNPYQCEAGYGHYPVKHTDDEDTDDGIIYMAGPGSAPGCSKAKTHCIHAQEHQSQNQHQNQNQNHTKATKCSYLAEEELASDYDEDVPAIDLSKATSVESFSTRVQTERSVNADGQPVTVTTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8Z7</accession>
    <name>A0A6I8W8Z7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC6902136</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6">MV-25-SWS-2005</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239858.1">
        <property type="nucleotide sequence ID" value="XM_033383967.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033239859.1">
        <property type="nucleotide sequence ID" value="XM_033383968.1"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007173">
        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050944">
        <property type="entry name" value="FAM83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181:SF29">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="693"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="2493"/>
            <end position="2522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="637"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="761"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="900"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1496"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033239977.1"/>
        </source>
    </evidence>
    <sequence length="286" mass="31882" checksum="9FBD10EE796E4CDE" modified="2020-08-12" version="1">MNTMKFNTTVIGFLVLSCMAAAQKNWKLWKPTNSVTIRQNGGSLCANHLVGEFVPHAEDCQMFYLCVENGDAVLASCPPTMLFNSDSKLCDTAGNVRCRNDTKSGMENAIPPNAGDNDFNNMITDAATYCATLTQQQSNDRIVYIGSSSSCSNYYICYYGQAILQECSSELHWNAMTGKCDVPERAQCTLGAPEEDPSTNPTAPNGNINSGVAVWNEFIHCPTYGQHLYPHMQRCEFFIYCVKGHPTLQQCPFYYFFDIASKSCQWSRTALCVRDLNLPPQIKRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WA29</accession>
    <name>A0A6I8WA29_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein gogo</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gogo</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033240142.1">
        <property type="nucleotide sequence ID" value="XM_033384251.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8WA29">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813827"/>
    <dbReference type="InParanoid" id="A0A6I8WA29"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000021">
        <property type="entry name" value="semaphorin-5B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038877">
        <property type="entry name" value="THSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16311">
        <property type="entry name" value="THROMBOSPONDIN TYPE I DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16311:SF3">
        <property type="entry name" value="THROMBOSPONDIN TYPE-1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="753"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="884"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1097"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1226"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1288"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1143"/>
            <end position="1177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033240142.1"/>
        </source>
    </evidence>
    <sequence length="1308" mass="143057" checksum="BC9221C9D29AD743" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCF8</accession>
    <name>A0A6I8WCF8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12 13 14 15 16" type="primary">Trf4-1</name>
    </gene>
    <organism evidence="10 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12 13" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12 13">MV-25-SWS-2005</strain>
            <tissue evidence="11 12 13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="3"/>
    <dbReference type="RefSeq" id="XP_033240934.1">
        <property type="nucleotide sequence ID" value="XM_033385043.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240935.1">
        <property type="nucleotide sequence ID" value="XM_033385044.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240936.1">
        <property type="nucleotide sequence ID" value="XM_033385045.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240937.1">
        <property type="nucleotide sequence ID" value="XM_033385046.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240938.1">
        <property type="nucleotide sequence ID" value="XM_033385047.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240940.1">
        <property type="nucleotide sequence ID" value="XM_033385049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCF8"/>
    <dbReference type="KEGG" id="dpo:4815218"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WCF8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000003">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="9">
        <location>
            <begin position="313"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="8">
        <location>
            <begin position="500"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="698"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="834"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="984"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1195"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="134"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="698"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="835"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="868"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="984"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1054"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1081"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1103"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1124"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1195"/>
            <end position="1208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033240934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033240935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_033240936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033240937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_033240938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033240940.1"/>
        </source>
    </evidence>
    <sequence length="1220" mass="132288" checksum="1501F1C24C39093F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCV8</accession>
    <name>A0A6I8WCV8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Keratin, type I cytoskeletal 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Twdlalpha</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241077.1">
        <property type="nucleotide sequence ID" value="XM_033385186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCV8"/>
    <dbReference type="FunCoup" id="A0A6I8WCV8">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815584"/>
    <dbReference type="InParanoid" id="A0A6I8WCV8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026051380" evidence="2">
        <location>
            <begin position="17"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="3">
        <location>
            <begin position="152"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033241077.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="36396" checksum="368B03739DE36037" modified="2020-08-12" version="1">MRIFVTLCTLMALGQAKPQQGYNYGTGLSGTLTHTQSSQGGGFLSAPSNTQSSVAYGPLGAFQSASVGSLVGSSGGGGGGGYSSGGSNYVSGSSSSNFGGGRTASSNYGGSSGGFTGFGPTPNIGFGALSGYQAPSYQQQQEQEVQRGFQEPIIHKQFFTVAAPEEHENLERAKHLVIGRPQKNYRVVFIKAPSSSNANVKLSAEYAPKEEKTVIYVLSKKDNQLEVNDIATPAPTVPSKPEVFFIKYKTEAEASHAQQQIQAEYDRIEGTSEHSDGGVAPAQSVVGILDGGALGSASSGSSQYAAGGGSSSGGSAGGYTSSTGGSAVGSIGGYTTTVSGGSSGGHNNAQSATYLPPSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WF79</accession>
    <name>A0A6I8WF79_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein DDB_G0290503</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241734.1">
        <property type="nucleotide sequence ID" value="XM_033385843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WF79"/>
    <dbReference type="FunCoup" id="A0A6I8WF79">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897006"/>
    <dbReference type="InParanoid" id="A0A6I8WF79"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19327">
        <property type="entry name" value="GOLGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19327:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="628"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="424"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="658"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241734.1"/>
        </source>
    </evidence>
    <sequence length="675" mass="77099" checksum="729DB9BF2725B3D1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8FAM5</accession>
    <name>A0ABD8FAM5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4814934</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001354537.2">
        <property type="nucleotide sequence ID" value="XM_001354501.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8FAM5"/>
    <dbReference type="SMR" id="A0ABD8FAM5"/>
    <dbReference type="GeneID" id="4814934"/>
    <dbReference type="KEGG" id="dpo:4814934"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106143">
        <property type="term" value="C:tRNA (m7G46) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000060">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="226"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001354537.2"/>
        </source>
    </evidence>
    <sequence length="247" mass="28537" checksum="F7A61C33120A9D48" modified="2025-06-18" version="1">MVTTTAENEVLTSTSAVTGLPQKRYYRQRAHSNPIADHCFDYPARPEDVDWRSLYPSIRADQEVSFADIGCGYGGFLVTLGELFPEKISIGMEIRVKVSDYVLDRITALRQKSSGTGAYQNIACLRTNAMKYLPNYFRKGQLEKMFFLYPDPHFKRAKHKWRIINQALLSEYAYVLRKGGLVYTMTDVEDLHNWIVTHMDEHPLYERLDEEEANTDPITPKLYQSSEEGAKVVRNKGDHFLAIFRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8H312</accession>
    <name>A0ABD8H312_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MAU2 chromatid cohesion factor homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cohesin loading complex subunit SCC4 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Mau2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for association of the cohesin complex with chromatin during interphase. Plays a role in sister chromatid cohesion and normal progression through prometaphase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with Nipped-B to form the cohesin loading complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCC4/mau-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359334.2">
        <property type="nucleotide sequence ID" value="XM_001359297.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8H312"/>
    <dbReference type="GeneID" id="4802411"/>
    <dbReference type="KEGG" id="dpo:4802411"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000373">
        <property type="entry name" value="MAU2 chromatid cohesion factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000915">
        <property type="entry name" value="MAU2 chromatid cohesion factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019440">
        <property type="entry name" value="MAU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21394">
        <property type="entry name" value="MAU2 CHROMATID COHESION FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10345">
        <property type="entry name" value="Cohesin_load"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001359334.2"/>
        </source>
    </evidence>
    <sequence length="620" mass="70145" checksum="B2209CDBB9D0F99B" modified="2025-06-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>B5DL62</accession>
    <accession>A0A6I8UYV7</accession>
    <name>B5DL62_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="7">6.2.1.4</ecNumber>
            <ecNumber evidence="7">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="7">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Scsalpha2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.2.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with an ATP-specific beta subunit, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with a GTP-specific beta subunit forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.4" evidence="7"/>
    <dbReference type="EC" id="6.2.1.5" evidence="7"/>
    <dbReference type="RefSeq" id="XP_002133634.2">
        <property type="nucleotide sequence ID" value="XM_002133598.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DL62"/>
    <dbReference type="SMR" id="B5DL62"/>
    <dbReference type="FunCoup" id="B5DL62">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DL62"/>
    <dbReference type="GeneID" id="6902103"/>
    <dbReference type="KEGG" id="dpo:6902103"/>
    <dbReference type="eggNOG" id="KOG1255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_1_0_1"/>
    <dbReference type="InParanoid" id="B5DL62"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244409">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DL62">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000005">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="10">
        <location>
            <begin position="35"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="7 8">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002133634.2"/>
        </source>
    </evidence>
    <sequence length="342" mass="36233" checksum="3855DE3B3CB2FA76" modified="2016-01-20" version="2">MTMSNWRASGWLAPARLLRVVRGKSDYSKTAKNLKINKETKLLVQGFTGKQATFHSQEALKYGTNVVGGVSPKKGGTKHLDKPVFKSVAEATKELKPDATVIFIPPSNAADGIRSAIESEIGLIVAITEGIPQVDMVRIMQMLNCQEKSRLLGPNCPGIISPDQCKIGIMPGDIHKRGCVGIVSRSGTLTYEAVQQTTKTGLGQTLCIGCGGDPFNGTNFIDGLKIFLSDPETKGIILIGEIGGSAEEDAAEFLKQHNSGCKAKPVVSFIAGQTAPPGRRMGHAGAIIAGGKGTAPAKVKALEKAGVIVSQNPCHMGSTLYKEMLRLKLVPKKEEAGSSNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>B5DNS6</accession>
    <accession>A0A6I8V041</accession>
    <name>B5DNS6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Basic helix-loop-helix transcription factor scleraxis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6901072</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134551.1">
        <property type="nucleotide sequence ID" value="XM_002134515.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DNS6"/>
    <dbReference type="SMR" id="B5DNS6"/>
    <dbReference type="FunCoup" id="B5DNS6">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DNS6"/>
    <dbReference type="GeneID" id="6901072"/>
    <dbReference type="KEGG" id="dpo:6901072"/>
    <dbReference type="eggNOG" id="KOG4029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141831_0_0_1"/>
    <dbReference type="InParanoid" id="B5DNS6"/>
    <dbReference type="OMA" id="RISICTF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243769">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF42">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="20"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002134551.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16035" checksum="5C121A67696992ED" modified="2008-10-14" version="1">MAISSAATASYLLMALTHDSHSHSHSQSPSDGSGSGGAGDSEDSQTGGRRRRPPRQKINARERYRTFNVNAAYEALRSMIPTEPINRKLSKIEIIRLASSYINHLSSTLQTGTDFQPCLLHKYENEGGGAGGHTRRISICTFCLKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>B5DP79</accession>
    <accession>A0A6I8V0A9</accession>
    <name>B5DP79_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Synaptic vesicle glycoprotein 2C-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6900921</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134703.2">
        <property type="nucleotide sequence ID" value="XM_002134667.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DP79"/>
    <dbReference type="STRING" id="46245.B5DP79"/>
    <dbReference type="GeneID" id="6900921"/>
    <dbReference type="KEGG" id="dpo:6900921"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_15_1"/>
    <dbReference type="InParanoid" id="B5DP79"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250665">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF37">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002134703.2"/>
        </source>
    </evidence>
    <sequence length="486" mass="54661" checksum="618D896989BF86EB" modified="2016-01-20" version="2">MADIDEVYGMLGFGRMQRTIFVCCILIQAYITNEQMGLALVVASSACDLHIDDHRMSWLMSAVFTAQILASHYMGDKADDIGRRKLMLISGSLMILSSLLSALMPEFWSFLVMRFLVGVFVSGPAVAMQTYLSEFTKFSLRPKVLNYVSYAIGFSMIIVPCTAALIDVNILIYNDYVFSSWRLLILLNLLPGLTAFVMLYRLPESPKYYLSVNENGKALAVLEECCRINKGKDVTLASLGVNSVTQPRLRGATIKRNCVARLWYESLPLLQKPYVRNFFLTSTAVFVQFGLSFGLGVWMLRIRHLILQVEEDETICVLIKEHGDKKMKLPTCDLRFENVMDSIYHGIFVLFLFILTSFLLMCSRRRTIILLYLLSAALAGFMCNFAQYDTLILIVFLIVIVPPICSLRLTLSLLIDLVPTHLRGKAVALALIFGRAGVLCASLFIGYTLRDHCYFTFNGFALAITVATIVILLLPSESKMMDRVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>B5DR17</accession>
    <accession>A0A6I8V188</accession>
    <name>B5DR17_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6900003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135368.1">
        <property type="nucleotide sequence ID" value="XM_002135332.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DR17"/>
    <dbReference type="GeneID" id="6900003"/>
    <dbReference type="KEGG" id="dpo:6900003"/>
    <dbReference type="eggNOG" id="ENOG502RJ1C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2361890_0_0_1"/>
    <dbReference type="InParanoid" id="B5DR17"/>
    <dbReference type="OMA" id="SCVKAKP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002135368.1"/>
        </source>
    </evidence>
    <sequence length="109" mass="11981" checksum="E7A66D8270C4053B" modified="2008-10-14" version="1">MRIYSYLWQQLMLVLIASICVKAKPLVSFGVSYQQPQPYVGPGPGAYYNSYYGGPGPYQSGYGYYSSSNHYYGSSPGASYPYRYPYAAGHRYGALDVGIGALSLTPFLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q291K8</accession>
    <name>SIN1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Stress-activated map kinase-interacting protein 1</fullName>
            <shortName>SAPK-interacting protein 1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Sin1</name>
        <name type="ORF">GA10075</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Component of the target of rapamycin complex 2 (TORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization and anabolic output. In response to growth factors, TORC2 phosphorylates and activates AGC protein kinase family members, such as Akt1 (By similarity). Within the TORC2 complex, Sin1 acts as a substrate adapter which recognizes and binds AGC protein kinase family members for phosphorylation by mTor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the target of rapamycin complex 2 (TORC2).</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SIN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL25104.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q291K8"/>
    <dbReference type="SMR" id="Q291K8"/>
    <dbReference type="FunCoup" id="Q291K8">
        <property type="interactions" value="1992"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q291K8"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0278417">
        <property type="protein sequence ID" value="FBpp0276855"/>
        <property type="gene ID" value="FBgn0070134"/>
    </dbReference>
    <dbReference type="GeneID" id="4803881"/>
    <dbReference type="KEGG" id="dpo:4803881"/>
    <dbReference type="CTD" id="36604"/>
    <dbReference type="eggNOG" id="KOG3739">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514767_0_0_1"/>
    <dbReference type="InParanoid" id="Q291K8"/>
    <dbReference type="OMA" id="NAKFWPQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070134">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032679">
        <property type="entry name" value="Sin1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05422">
        <property type="entry name" value="SIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0053">Apoptosis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000331443" description="Stress-activated map kinase-interacting protein 1">
        <location>
            <begin position="1"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="184"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="3">
        <location>
            <begin position="398"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9BPZ7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9V719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="578" mass="65402" checksum="4225FE2258F2279F" modified="2009-01-20" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-03-06" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q299G2</accession>
    <name>CYFIP_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytoplasmic FMR1-interacting protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Specifically Rac1-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">Cyfip</name>
        <name evidence="1" type="synonym">Sra-1</name>
        <name type="ORF">GA18534</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in guidance and morphology of central and peripheral axons and in synaptic morphology. Also required for formation of cell membrane protrusions and for bristle development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with Fmr1 and Rac1. Component of the WAVE complex composed of Hem/Kette, Scar/Wave and Cyfip where it binds through its C-terminus directly to Hem.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Accumulates in central axons and motor neuron terminals.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYFIP family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL27741" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL27741.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001358600.1">
        <property type="nucleotide sequence ID" value="XM_001358563.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q299G2"/>
    <dbReference type="SMR" id="Q299G2"/>
    <dbReference type="FunCoup" id="Q299G2">
        <property type="interactions" value="1641"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q299G2"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0285411">
        <property type="protein sequence ID" value="FBpp0283849"/>
        <property type="gene ID" value="FBgn0078536"/>
    </dbReference>
    <dbReference type="GeneID" id="4801524"/>
    <dbReference type="KEGG" id="dpo:4801524"/>
    <dbReference type="CTD" id="41861"/>
    <dbReference type="eggNOG" id="KOG3534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002688_2_1_1"/>
    <dbReference type="InParanoid" id="Q299G2"/>
    <dbReference type="OMA" id="DQPNRVE"/>
    <dbReference type="PhylomeDB" id="Q299G2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078536">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022416">
        <property type="term" value="P:chaeta development"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050807">
        <property type="term" value="P:regulation of synapse organization"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009828">
        <property type="entry name" value="CYRIA/CYRIB_Rac1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008081">
        <property type="entry name" value="Cytoplasmic_FMR1-int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12195">
        <property type="entry name" value="CYTOPLASMIC FMR1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05994">
        <property type="entry name" value="FragX_IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008153">
        <property type="entry name" value="FMR1_interacting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01698">
        <property type="entry name" value="CYTOFMRPINTP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0221">Differentiation</keyword>
    <keyword id="KW-0524">Neurogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000279715" description="Cytoplasmic FMR1-interacting protein">
        <location>
            <begin position="1"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1269"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1271"/>
            <end position="1291"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9VF87"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="EAL27741.1"/>
        </source>
    </evidence>
    <sequence length="1291" mass="149362" checksum="4892892A9F05C030" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EM9</accession>
    <accession>A0A6I8UAR8</accession>
    <name>Q29EM9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytochrome b5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC4812173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352534.1">
        <property type="nucleotide sequence ID" value="XM_001352498.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EM9"/>
    <dbReference type="SMR" id="Q29EM9"/>
    <dbReference type="FunCoup" id="Q29EM9">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EM9"/>
    <dbReference type="GeneID" id="4812173"/>
    <dbReference type="KEGG" id="dpo:4812173"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102602_4_0_1"/>
    <dbReference type="InParanoid" id="Q29EM9"/>
    <dbReference type="OMA" id="PCWLIIK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078697">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF95">
        <property type="entry name" value="CYTOCHROME B5 TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="82"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001352534.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="14424" checksum="C7E73A1A9C9F9DFB" modified="2006-04-04" version="1">MSQLYELSEVAEQNGKKGKPCWIIIKGNVYDVTKFLSEHPGGEDLLLEYGGKDASKAFRQAGHSSDAEKDLKNFKIGELRATTTPTTTTTTTAPIQVQPTLKDQDKEKRVSSPVPADDAEPMKKSSGFLCCC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2025-06-18" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q29EX2</accession>
    <name>EIF3E_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Eukaryotic translation initiation factor 3 subunit E</fullName>
            <shortName evidence="2">eIF3e</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Eukaryotic translation initiation factor 3 subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">eIF3-S6</name>
        <name type="synonym">Int6</name>
        <name type="ORF">GA21959</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. Interacts with mxt.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-3 subunit E family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379070">
        <property type="protein sequence ID" value="EAL29937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29EX2"/>
    <dbReference type="SMR" id="Q29EX2"/>
    <dbReference type="FunCoup" id="Q29EX2">
        <property type="interactions" value="2627"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29EX2"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0288106">
        <property type="protein sequence ID" value="FBpp0286544"/>
        <property type="gene ID" value="FBgn0081944"/>
    </dbReference>
    <dbReference type="GeneID" id="4812055"/>
    <dbReference type="KEGG" id="dpo:4812055"/>
    <dbReference type="CTD" id="3646"/>
    <dbReference type="eggNOG" id="KOG2758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031132_0_0_1"/>
    <dbReference type="InParanoid" id="Q29EX2"/>
    <dbReference type="OMA" id="NCPWILR"/>
    <dbReference type="PhylomeDB" id="Q29EX2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081944">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21378">
        <property type="entry name" value="eIF3E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03004">
        <property type="entry name" value="eIF3e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019010">
        <property type="entry name" value="eIF3e_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09440">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016255">
        <property type="entry name" value="eIF3e_su6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01186">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000365968" description="Eukaryotic translation initiation factor 3 subunit E">
        <location>
            <begin position="1"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="219"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O77410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01185"/>
        </source>
    </evidence>
    <sequence length="434" mass="51037" checksum="B5F002505FDC1E8B" modified="2006-04-04" version="1">MANFDLTRINCQYLDRHLTFPLLEFLCGKEIYNQQELLEYILETVNKTNMIDYTMDTRKRLNLSQEMPDELVQRKAEVLATLKQLQNEVAPIMKATDILKNGESMKDSKTFVNALQKDYNFKVEHLESAYKLAKYLYECGNYQESTSYLYFCLIVMSPNDKNYLNVLWGKLAAEILTLNWNTALEDLTRLRDYIDSANFSTIQALQQRTWLIHWSVLVFFNHPKGRDLIIEMFLYKPLYLNAIQTMCPHIMRYLATAVVINRTRRNALKDLIKVIQQESYTYRDPITEFLECLYVNFDFEGARLKLHECQTVILNDFFIVACLNEFVEDARLMIFETFCRIHQCITISMLADKLNMKPNEAECWIVNLIRNARLNAKIDSKLGHVVMGTQPLSPYQQLVEKIDSLSMRSEHLAGLIERKSKQNNKESIDSWKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GB6</accession>
    <accession>A0A6I8VIY9</accession>
    <name>Q29GB6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">RpL36</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355137.1">
        <property type="nucleotide sequence ID" value="XM_001355101.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015042101.1">
        <property type="nucleotide sequence ID" value="XM_015186615.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GB6"/>
    <dbReference type="STRING" id="46245.Q29GB6"/>
    <dbReference type="GeneID" id="4814716"/>
    <dbReference type="KEGG" id="dpo:4814716"/>
    <dbReference type="eggNOG" id="KOG3452">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140672_0_0_1"/>
    <dbReference type="OMA" id="NKGHKTE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080481">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000004">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001355137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015042101.1"/>
        </source>
    </evidence>
    <sequence length="115" mass="13506" checksum="741DE65C80B21B0D" modified="2006-04-04" version="1">MAVRYELCIGLNKGHKTTKVKNVKYTGDKKVKGLRGSRLKNIQTRHTKFMRDLVREVVGHAPYEKRTMELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNILTQLRKAQTHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JN9</accession>
    <accession>A0A6I8UI47</accession>
    <name>Q29JN9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Pickpocket protein 19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ppk7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355863.2">
        <property type="nucleotide sequence ID" value="XM_001355827.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JN9"/>
    <dbReference type="FunCoup" id="Q29JN9">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="GeneID" id="4816244"/>
    <dbReference type="KEGG" id="dpo:4816244"/>
    <dbReference type="eggNOG" id="KOG4294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024950_3_1_1"/>
    <dbReference type="InParanoid" id="Q29JN9"/>
    <dbReference type="OMA" id="PGQYRSC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000010">
        <property type="entry name" value="Pickpocket 23, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF288">
        <property type="entry name" value="AMILORIDE-SENSITIVE NA+ CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355863.2"/>
        </source>
    </evidence>
    <sequence length="572" mass="66929" checksum="DA8645E302DEB2F7" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LG4</accession>
    <accession>A0A6I8W045</accession>
    <name>Q29LG4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Zinc finger matrin-type protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC117184192</name>
        <name evidence="7" type="synonym">LOC4817613</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357015.2">
        <property type="nucleotide sequence ID" value="XM_001356979.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236628.1">
        <property type="nucleotide sequence ID" value="XM_033380737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LG4"/>
    <dbReference type="STRING" id="46245.Q29LG4"/>
    <dbReference type="GeneID" id="4817613"/>
    <dbReference type="KEGG" id="dpo:117184192"/>
    <dbReference type="KEGG" id="dpo:4817613"/>
    <dbReference type="eggNOG" id="KOG3454">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100385_1_1_1"/>
    <dbReference type="OMA" id="DYCCCFM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076583">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LG4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16465">
        <property type="entry name" value="NUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16465:SF0">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001357015.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033236628.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="16241" checksum="E3439B9812B85FAB" modified="2008-10-14" version="2">MGQSYFCDYCGCFMKSDLNVRKLHNNGISHTVAKYRYMRRFEDPAKILAEERLKKPCQRYFKGYCKFDLYCNYGHYSEREFKKLEKLVGAQKNSKTRKRKAKKWPWRTHQIGLPPSLRPMELAKLKKTNFELNWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q29M77</accession>
    <accession>A0A6I8UK10</accession>
    <name>Q29M77_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">dbe</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Binds to RNA. Required for female germline development, cell viability during eye development and for survival of dividing cells and epithelial cells during early wing disk development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer. Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
            <location evidence="1 12">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the KRR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356752.1">
        <property type="nucleotide sequence ID" value="XM_001356716.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29M77"/>
    <dbReference type="FunCoup" id="Q29M77">
        <property type="interactions" value="1788"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29M77"/>
    <dbReference type="GeneID" id="4817296"/>
    <dbReference type="KEGG" id="dpo:4817296"/>
    <dbReference type="eggNOG" id="KOG2874">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040185_0_2_1"/>
    <dbReference type="InParanoid" id="Q29M77"/>
    <dbReference type="OMA" id="TPDIDKW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078070">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29M77">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 12" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 12" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="14">
        <location>
            <begin position="124"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="228"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="271"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="275"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001356752.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="39229" checksum="AD5E1FD46CE43883" modified="2006-04-04" version="1">MSDSEEEIKQNTDPVDNAWSLKIPAFRQEDNPHGMVEESSFATLFPKYREKYLREVWPLVEQCVAEHQLKAELDLVEGSMVVKTTRKTWDPYIIIKSRDMIKLMARSVPFEQAKRVLQDDIGCDIIKIGNLVHKKEKFVKRRQRLIGPNGATLKSIELLTDCYVLVQGNTVSALGPYKGLQQVRDIVLETMNNVHPIYNIKALMIKRELMKDPKLANEDWSRFLPKFKNKNISKRKQPKAKKPKKEYTPFPPAQPESKIDKQLASGEYFLNQEQKQAKRNQERSAKQADAAKKQDERRNKDFVPPTEEAPSRKRQAEDSSKVDVKALKAKLVKANKKSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q29PJ1</accession>
    <accession>A0A6I8UL38</accession>
    <name>Q29PJ1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Dnz1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_001357233.1">
        <property type="nucleotide sequence ID" value="XM_001357197.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29PJ1"/>
    <dbReference type="SMR" id="Q29PJ1"/>
    <dbReference type="FunCoup" id="Q29PJ1">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="GeneID" id="4818085"/>
    <dbReference type="KEGG" id="dpo:4818085"/>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048061_0_0_1"/>
    <dbReference type="InParanoid" id="Q29PJ1"/>
    <dbReference type="OMA" id="CGKSHPI"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079731">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29PJ1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="98"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001357233.1"/>
        </source>
    </evidence>
    <sequence length="275" mass="31334" checksum="DCF4F7E12DB918A5" modified="2006-04-04" version="1">MVFIRDPCGIACLVMTYGAVLYADYVVIRWIILTTMPGSIWTSFHVVLFNTVVFLLGMSHTKAVFSDPGIVPLPANRLDFSDLHTTNKNNPVGNGHSSEWTVCTRCETYRPPRAHHCRICKRCIRRMDHHCPWINNCVGERNQKYFLQFLIYVAVLSLYSLGLIVASWVSPCEECNQNVIESQLRMIHSVILMLVSALFGLFVTAIMVDQLHAILYDETAVEAIQQKGTYRPNRRKYQLLADVFGRGHPALWLLPCTSLNHASRYHDTPLLSHDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LYP4</accession>
    <accession>A0A6I8UE54</accession>
    <name>Q2LYP4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Probable serine hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4813644</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354079.2">
        <property type="nucleotide sequence ID" value="XM_001354043.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LYP4"/>
    <dbReference type="SMR" id="Q2LYP4"/>
    <dbReference type="STRING" id="46245.Q2LYP4"/>
    <dbReference type="ESTHER" id="drops-q2lyp4">
        <property type="family name" value="SERHL"/>
    </dbReference>
    <dbReference type="GeneID" id="4813644"/>
    <dbReference type="KEGG" id="dpo:4813644"/>
    <dbReference type="eggNOG" id="KOG1454">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_8_1_1"/>
    <dbReference type="InParanoid" id="Q2LYP4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070962">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LYP4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF14">
        <property type="entry name" value="SERINE HYDROLASE-LIKE PROTEIN DDB_G0286239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001354079.2"/>
        </source>
    </evidence>
    <sequence length="356" mass="40976" checksum="7FE7AF84D8B30641" modified="2016-01-20" version="2">MEARKVHNMDFQEDSSSGTTGNDLGQRYRHAGDLTGEPPTRPFAEISITVPWGHISGKWYGPQNVQPILGLHGWQDNAGTFDLLMPLLSPDVAFLSIDLPGHGLSSRLPDGCYYNSVDNLYVIRLIMKQYKWEKVSLVGHSMSSIICFVFAAVFPDKVDMIIGIDALKPHQRPYPSVIRTMETRLDEFLREDERNRSKNEPPSYTYDEIIERVYLGTFHSVNKEHCKHMMARNIQKSEKYPDKYFFCRDRRLKFYNYAIGSQELCVEMAHRIKCPYMFIKATQSSYFEDKKYYDEVLAVLLKKPNFEYVEANGSHHVHMNSPEAIIEPVNSFIQRFGPAAAAAERQKAKEAKESKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q8I1C0</accession>
    <name>SH3G3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Endophilin-A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>SH3 domain-containing GRB2-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">EndoA</name>
        <name type="ORF">GA12885</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Genome Biol." volume="3" first="RESEARCH0086.1" last="RESEARCH0086.20">
            <title>Assessing the impact of comparative genomic sequence data on the functional annotation of the Drosophila genome.</title>
            <authorList>
                <person name="Bergman C.M."/>
                <person name="Pfeiffer B.D."/>
                <person name="Rincon-Limas D.E."/>
                <person name="Hoskins R.A."/>
                <person name="Gnirke A."/>
                <person name="Mungall C.J."/>
                <person name="Wang A.M."/>
                <person name="Kronmiller B."/>
                <person name="Pacleb J.M."/>
                <person name="Park S."/>
                <person name="Stapleton M."/>
                <person name="Wan K.H."/>
                <person name="George R.A."/>
                <person name="de Jong P.J."/>
                <person name="Botas J."/>
                <person name="Rubin G.M."/>
                <person name="Celniker S.E."/>
            </authorList>
            <dbReference type="PubMed" id="12537575"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">Tucson 14011-0121.4</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Associated with internal membranes. Expressed presynaptically at NMJs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="EMBL" id="AY190947">
        <property type="protein sequence ID" value="AAO01051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CM000070">
        <property type="protein sequence ID" value="EAL28006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001358863.1">
        <property type="nucleotide sequence ID" value="XM_001358826.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8I1C0"/>
    <dbReference type="SMR" id="Q8I1C0"/>
    <dbReference type="FunCoup" id="Q8I1C0">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q8I1C0"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0285605">
        <property type="protein sequence ID" value="FBpp0284043"/>
        <property type="gene ID" value="FBgn0064416"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0380938">
        <property type="protein sequence ID" value="FBpp0341278"/>
        <property type="gene ID" value="FBgn0064416"/>
    </dbReference>
    <dbReference type="GeneID" id="4801830"/>
    <dbReference type="KEGG" id="dpo:4801830"/>
    <dbReference type="CTD" id="42265"/>
    <dbReference type="eggNOG" id="KOG1118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047887_0_0_1"/>
    <dbReference type="InParanoid" id="Q8I1C0"/>
    <dbReference type="OMA" id="MFPANYC"/>
    <dbReference type="PhylomeDB" id="Q8I1C0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0064416">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q8I1C0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050803">
        <property type="term" value="P:regulation of synapse structure or activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07592">
        <property type="entry name" value="BAR_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000021">
        <property type="entry name" value="Endophilin-A2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF81">
        <property type="entry name" value="ENDOPHILIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0254">Endocytosis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0728">SH3 domain</keyword>
    <feature type="chain" id="PRO_0000285839" description="Endophilin-A">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="BAR" evidence="4">
        <location>
            <begin position="18"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="305"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8T390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAO01051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="EAL28006.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="41387" checksum="21DCE7FFF4EED940" modified="2003-03-01" version="1">MAFAGLKKQINKANQYMTEKMGGAEGTKLDMDFMDMERKTDVTVELVEELQLKTKEFLQPNPTARAKMAAVKGISKLSGQAKSNTYPQPEGLLAECMLTYGKKLGEDNSVFAQALVEFGESLKQMADVKYSLDDNIKQNFLEPLHHMQMKDLKEVMHHRKKLQGRRLDFDCKRRRQAKDDEIRGAEDKFAESLQLAQVGMFNLLENDTEHVSQLVTFAEALYDFHSQCADVLRGLQETLQEKRSEAESRPRNEFVPKTLLDLNLDGGGGGLIDDGTPSHISSSASPLPSPMRSPAKSMAVTPSRQQQPCCQALYDFDPENPGELGFKENDIITLLNRVDDNWYEGAVNGRTGYFPQSYVQVQVPLPNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NRL8</accession>
    <accession>A0A6I8V8U0</accession>
    <name>A0A0R3NRL8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glutamine synthetase</fullName>
            <ecNumber evidence="3 9">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Gs1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_015035633.1">
        <property type="nucleotide sequence ID" value="XM_015180147.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NRL8"/>
    <dbReference type="SMR" id="A0A0R3NRL8"/>
    <dbReference type="FunCoup" id="A0A0R3NRL8">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="GeneID" id="117183175"/>
    <dbReference type="KEGG" id="dpo:117183175"/>
    <dbReference type="InParanoid" id="A0A0R3NRL8"/>
    <dbReference type="OMA" id="HAVACLY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246914">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NRL8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF44">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="10">
        <location>
            <begin position="62"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="11">
        <location>
            <begin position="150"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_015035633.1"/>
        </source>
    </evidence>
    <sequence length="400" mass="44498" checksum="39BF365C7CC375C1" modified="2016-01-20" version="1">MAFRVAGLFLKKELVAPATQQLRLFRTSKTAWSTFLVNSPNTALDKSILQRYRNLETPANRVQATYLWIDGTGENIRLKDRVLDKVPSSVEDLPDWQYDGSSTYQAHGENSDTTLKPRAIYRDPFKPGRNDVIVLCDTYNADGKPTPSNKRAAFQAAVDKIGDQEPWFGIEQEYTLLDVDGRPFGWPDNGFPAPQGPYYCGVGADRVYARDLVEAHTVACLYAGIDFAGTNAEVMPAQWEYQVGPSNGLKASDDLWVSRYILQRIAEEYGVVVTFDPKPMEGQWNGAGAHTNFSTKAMRADGGIKAIEAAIEKLSKQHERHIKAYDPKEGKDNERRLVGRLETSSIDKFSWGVANRAVSVRVPRGVATAGKGYFEDRRPSSNCDPYAVCGALVRTCLLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UBS1</accession>
    <name>A0A6I8UBS1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Corepressor interacting with RBPJ 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4811854</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352908.2">
        <property type="nucleotide sequence ID" value="XM_001352872.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UBS1"/>
    <dbReference type="FunCoup" id="A0A6I8UBS1">
        <property type="interactions" value="972"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4811854"/>
    <dbReference type="InParanoid" id="A0A6I8UBS1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040014">
        <property type="entry name" value="CIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13151">
        <property type="entry name" value="CBF1 INTERACTING COREPRESSOR CIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13151:SF2">
        <property type="entry name" value="COREPRESSOR INTERACTING WITH RBPJ 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="302"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001352908.2"/>
        </source>
    </evidence>
    <sequence length="370" mass="44044" checksum="B1C6CFB9CB218F79" modified="2020-08-12" version="1">MGKGFNNYMCKKFFHPASRDNLKRVWMAEQQADAYKKKQEELRNQYEKEQNLHENKAMLSKDSKDKLSVNFMYEPPPGVRKEREKDDDEPEYKFEWQRKYNAPRESYCKGDTEIRDQPFGIQVRNVRCLKCHKWGHINTDKECSMYSISMSEARKLHAESEATDIMSKNVLEEQMKEDGLMMKRSALELQSIKTIQKQHQLVPSDEEDEANANKQNPELMFLKSLTKKQKLKLLKKLEKIDRMKRKGESIKKPSKKKSKKPNEDRDTKKKKSKSSQKERSARKERKSVDSDGSSSSSDGDSEDNHRSHHKDQQPREPQRKNGTHRSRESEDGLQRRRNDRSQRRERSRDRSRSASGERRRERDRRDYSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCB7</accession>
    <name>A0A6I8UCB7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4813920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353222.3">
        <property type="nucleotide sequence ID" value="XM_001353186.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCB7"/>
    <dbReference type="KEGG" id="dpo:4813920"/>
    <dbReference type="InParanoid" id="A0A6I8UCB7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCB7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353222.3"/>
        </source>
    </evidence>
    <sequence length="231" mass="26161" checksum="A1E9FEE5CF12E3B1" modified="2020-08-12" version="1">MATAIPMLLDAIFLIPRLFTYGLILLLICIMCFIVVYISHKIYISVYDNLPLSGHQPMPMPMPMPLGAIRDYIDSPVRHLQSVQRATSRSSAHQTSSMCDRSISLQNRSQFIQSTTVRITLSLEPCYLVGIKFDMSPSRRSISFTCSCDREPPVCVCAAVTDRPSESVRIIRYYDQLSKISRLKFMLKCSHSPTMPTMPTIPTMLREEQPSDRPGQHHVATEKYPNSGDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCH8</accession>
    <name>A0A6I8UCH8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 337 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC4812965</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353219.3">
        <property type="nucleotide sequence ID" value="XM_001353183.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCH8"/>
    <dbReference type="FunCoup" id="A0A6I8UCH8">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812965"/>
    <dbReference type="InParanoid" id="A0A6I8UCH8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UCH8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001370">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001353219.3"/>
        </source>
    </evidence>
    <sequence length="472" mass="54183" checksum="E7E57CE6FB9180CB" modified="2020-08-12" version="1">MCIYRVFNEQNKRLKPNNTQLFIYYLIKEDMEVQHRIVFVCSLCLRQYDLMEDLRGHMVFYHGCQPVAGNKAVKTPTKAESNASRCTLEKKTNENQVEIAAPPPTDLQPMPFKDFRLVLRANMRVPCSASKECLFKFEDASKMEVHRRCHKGSSFACCECGLEMVNWRRCSAHLWKVHQIDVDLLRCPALDCTYNAPVSALVWRHMRVHKKWRSRVLRSLAAVQRRRKLKDQSDELPSQPTATTATPKMNKYYAEKTCEICNRRFVNGKTLSKHVKTVHNKIKPFICNVCGKKTARKASLIIHMRQHTGEKPLQCEQCKFSTRDPSVLHKHRQRHGSASKQSTLKCGHCDYGCIQANALKRHMRMNHADAYRDLCCDLCSYTSINAKRLQAHKQDHRQGLITNCEDSMDAARAAGFKYPRKLSDKNNEISADCFMPLESVDSMPHEPAVDTGGVTIPAPTSDDTQFPTYLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UDS7</accession>
    <name>A0A6I8UDS7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Allatostatins MIP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Mip</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353804.3">
        <property type="nucleotide sequence ID" value="XM_001353768.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UDS7"/>
    <dbReference type="FunCoup" id="A0A6I8UDS7">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813415"/>
    <dbReference type="InParanoid" id="A0A6I8UDS7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026060345" evidence="2">
        <location>
            <begin position="29"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353804.3"/>
        </source>
    </evidence>
    <sequence length="211" mass="23628" checksum="A3151B5A9F1AAAAB" modified="2020-08-12" version="1">MAHTKTKTQNRILLVVVLLLCSACSSLATGSIASTASNEPSGLSEQVVDQMSDSELYSNNKRAWQSLQSSWGKRSRDGPDGSEASFGGDDNIYMTGHFVPLVITDGTNTIDWDTFERLASNQDMQPQTLQQQQSGEDMVEDPEAAGNMEKRAWKSMNVAWGKRRQAQGWNKFRGAWGKREPTWNNLKGMWGKRDQWQKLHGGWGKRSMALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFH8</accession>
    <name>A0A6I8UFH8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable ATP-dependent RNA helicase ddx17 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CDK2AP1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDK2AP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354689.3">
        <property type="nucleotide sequence ID" value="XM_001354653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFH8"/>
    <dbReference type="FunCoup" id="A0A6I8UFH8">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815005"/>
    <dbReference type="InParanoid" id="A0A6I8UFH8"/>
    <dbReference type="OMA" id="ANMCSTN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074905">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017266">
        <property type="entry name" value="DOC_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22607:SF3">
        <property type="entry name" value="CDK2-ASSOCIATED PROTEIN 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22607">
        <property type="entry name" value="DELETED IN ORAL CANCER 1/CDK2-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09806">
        <property type="entry name" value="CDK2AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="52"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001354689.3"/>
        </source>
    </evidence>
    <sequence length="282" mass="29583" checksum="A0D03C3450E338B9" modified="2020-08-12" version="1">MDIMDIQAVESKLSDVTVTPIPRSQVQNFYNYQQQREQREQQPQIQISAIHHSRGNSSNSGSAGSSGNNSMGGMGATDYSTSSGSGNANSNKRDRSADYSTSSKQNSAAANAAAAAAVAALQYSPQFLQAQLAMLQQQSNTTATPAAAAAAALSLANIAAATNGARNSGNSNGNNQGLNLTQSQLKYPPPSTSPVVVTTQTSANITTPLTSTANLPTVGSLSTGNGLTKYAQLLAVIEEMGRDIRPTYTGSRSSTERLKRGIVHARILVRECLMETERAARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFN7</accession>
    <name>A0A6I8UFN7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">WD repeat-containing protein 46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC4814991</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001354760.2">
        <property type="nucleotide sequence ID" value="XM_001354724.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFN7"/>
    <dbReference type="FunCoup" id="A0A6I8UFN7">
        <property type="interactions" value="2140"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4814991"/>
    <dbReference type="InParanoid" id="A0A6I8UFN7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFN7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001016">
        <property type="entry name" value="WD repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012952">
        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040315">
        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08149">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01033">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="BING4 C-terminal" evidence="7">
        <location>
            <begin position="422"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="568"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001354760.2"/>
        </source>
    </evidence>
    <sequence length="609" mass="68450" checksum="BC648295154EDBA5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UH58</accession>
    <name>A0A6I8UH58_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Trypsin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4815734</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355437.4">
        <property type="nucleotide sequence ID" value="XM_001355401.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UH58"/>
    <dbReference type="KEGG" id="dpo:4815734"/>
    <dbReference type="InParanoid" id="A0A6I8UH58"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UH58">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000047">
        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026192023" evidence="9">
        <location>
            <begin position="22"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="26"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001355437.4"/>
        </source>
    </evidence>
    <sequence length="282" mass="29524" checksum="AD56EC16AF002C0D" modified="2020-08-12" version="1">MVLCGPIGLLLLTSVVVGTFADDGKIVNGTTASPGEFPFVVSLRRAKSGHHSCGATLLNSDWVLTAAHCVRGSTPQQLNLQYGSQSLARNASKLVQVAAIHVHPGYEPHDKYVNDIALLQLKDPVVLGARVQPVRLPEPEQPTLGNSSAVLAGWGLNATGGAVQLQLQKVQLQVFSDVDCSKRHQTQLHSTQICAGVPEGGKGQCSGDSGGPLLLAGSDTQIGIVSWSVKPCARPPYPGVFTEVSAYVNWIVETVGASSPTPSSSLWIGQLIVGRTPPVLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHB4</accession>
    <name>A0A6I8UHB4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Dual specificity protein phosphatase MPK-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MKP-4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 17">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355495.3">
        <property type="nucleotide sequence ID" value="XM_001355459.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHB4"/>
    <dbReference type="FunCoup" id="A0A6I8UHB4">
        <property type="interactions" value="2311"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815769"/>
    <dbReference type="InParanoid" id="A0A6I8UHB4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000056">
        <property type="entry name" value="Dual specificity phosphatase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016278">
        <property type="entry name" value="DUSP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45848">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000941">
        <property type="entry name" value="DUSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="27"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="91"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="240"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="11">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000941-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001355495.3"/>
        </source>
    </evidence>
    <sequence length="380" mass="43016" checksum="D082FEDAE7E723FC" modified="2020-08-12" version="1">MAQKQQARSSGKPQDSGSLTREDFDGGPVSIDEIETGLFLGNLTAATHMETLKSFKITHILTLDSVPLPQHILEASFLTTKYIQIADMPREDILQHLESCVNFISSALDQQGNVLVHCYFGVSRSSSTVIAYMMKRHNLDFQPAYELVRGKRRFVQPNSGFITQLKLFRRMGCKIDPSYQRYKMHRLRLAGEQMRKAKILPQSFHSVVRPDPDITRENPEPIVFRCRRCRRVLASKSHVLEHKPRDRLPREGTSTAVPNEDASGETPPPTQTQADNISAPRMLEQVAERIRQSSLGSPGHESTPNHCRSMLFIEPIAWMHRIMLNTQGRLYCPKCEQKLGNFSWINACQCPCGETLSPAFYLIPSKVELSKAVQNVQTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHD8</accession>
    <name>A0A6I8UHD8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 18 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">dor</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355427.2">
        <property type="nucleotide sequence ID" value="XM_001355391.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UHD8"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="term" value="P:vesicle docking involved in exocytosis"/>
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        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
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        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
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        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 18 HOMOLOG"/>
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        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="417"/>
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            <begin position="648"/>
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    </feature>
    <feature type="domain" description="Pep3/Vps18 RING C-terminal" evidence="11">
        <location>
            <begin position="880"/>
            <end position="981"/>
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    <feature type="coiled-coil region" evidence="9">
        <location>
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    <evidence type="ECO:0000256" key="1">
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000259" key="10">
        <source>
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        <source>
            <dbReference type="Pfam" id="PF26148"/>
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    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001355427.2"/>
        </source>
    </evidence>
    <sequence length="1003" mass="115082" checksum="F2E564A0A438259E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHV1</accession>
    <name>A0A6I8UHV1_DROPS</name>
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        <recommendedName>
            <fullName evidence="4 12">Glutathione synthetase</fullName>
            <shortName evidence="12">GSH-S</shortName>
            <ecNumber evidence="3 12">6.3.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Gss2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13558"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12 14">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001355684.4"/>
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        <property type="reaction ID" value="UER00210"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Glutathione synthetase"/>
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    <dbReference type="FunFam" id="3.30.1490.50:FF:000002">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1760:FF:000007">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1760">
        <property type="entry name" value="Glutathione synthase, substrate-binding domain superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1080.10">
        <property type="entry name" value="Glutathione Synthetase, Chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005615">
        <property type="entry name" value="Glutathione_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014042">
        <property type="entry name" value="Glutathione_synthase_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014709">
        <property type="entry name" value="Glutathione_synthase_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014049">
        <property type="entry name" value="Glutathione_synthase_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037013">
        <property type="entry name" value="GSH-S_sub-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004887">
        <property type="entry name" value="GSH_synth_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01986">
        <property type="entry name" value="glut_syn_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130:SF0">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03917">
        <property type="entry name" value="GSH_synth_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03199">
        <property type="entry name" value="GSH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001558">
        <property type="entry name" value="GSHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutathione synthase substrate-binding" evidence="16">
        <location>
            <begin position="293"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="161"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="455"/>
            <end position="464"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="488"/>
            <end position="491"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="542"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="544"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR001558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001355720.4"/>
        </source>
    </evidence>
    <sequence length="574" mass="62887" checksum="A8F914D77DDEDB29" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UHV4</accession>
    <name>A0A6I8UHV4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HEAT repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">l(2)k09022</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355765.2">
        <property type="nucleotide sequence ID" value="XM_001355729.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UHV4">
        <property type="interactions" value="2051"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816210"/>
    <dbReference type="InParanoid" id="A0A6I8UHV4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012954">
        <property type="entry name" value="BP28_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056473">
        <property type="entry name" value="HEAT_Utp10/HEAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022125">
        <property type="entry name" value="U3snoRNP10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08146">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23243">
        <property type="entry name" value="HEAT_HEATR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12397">
        <property type="entry name" value="U3snoRNP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01036">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BP28 C-terminal" evidence="8">
        <location>
            <begin position="1820"/>
            <end position="1970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001355765.2"/>
        </source>
    </evidence>
    <sequence length="2100" mass="237559" checksum="5D958CD99FD081E2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIR2</accession>
    <name>A0A6I8UIR2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Xenotropic and polytropic retrovirus receptor 1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4816609</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356122.3">
        <property type="nucleotide sequence ID" value="XM_001356086.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIR2"/>
    <dbReference type="FunCoup" id="A0A6I8UIR2">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816609"/>
    <dbReference type="InParanoid" id="A0A6I8UIR2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14477">
        <property type="entry name" value="SPX_XPR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF127">
        <property type="entry name" value="LD30826P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="538"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="10">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="9">
        <location>
            <begin position="419"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="631"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="640"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356122.3"/>
        </source>
    </evidence>
    <sequence length="654" mass="77042" checksum="C01F99552872F705" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJ37</accession>
    <name>A0A6I8UJ37_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4816624</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in vascular wall and kidney homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_001356181.3">
        <property type="nucleotide sequence ID" value="XM_001356145.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJ37"/>
    <dbReference type="FunCoup" id="A0A6I8UJ37">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816624"/>
    <dbReference type="InParanoid" id="A0A6I8UJ37"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UJ37">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000075">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 12" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026379952" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356181.3"/>
        </source>
    </evidence>
    <sequence length="426" mass="48201" checksum="C28470F2D189A3C4" modified="2020-08-12" version="1">MVWGSFMLVCVLALTSVVGARQGFGPGDQDWGYVDVREGAHMFYWLYYTTANVSHYTERPLVLWLQGGPGGASSALGNFMELGPVDMHGEPREGNWVQHVNLLFIDSPVGSGYSYVDNTSLLVANNEELTEDLMTFMLYFYKTHKEFKSVPLHIFTESYGGKMAPALALKMDLAMKSGELAEPNTLKSVSMGNPWISTRHISREHSKYMLVNGLIDEDGAKLIDAQEEKILIALKVHKFEMATDEYLEWFDLMQNLTGEVYLYNTQTHVDPEEDRTYGYGEDLNRFMQENISEALQINGSIYKAQVMDVFGTLHGDRLKSEINTIPRLLNETSIKINIFSGQLDALVPTTATLAVIKDWAWRDKEEYVQARRTPIFVADRLQGYEKVGGRFGMYWINRSGHQAPADNPTAMQYVLKAVTQYNESAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJ45</accession>
    <name>A0A6I8UJ45_DROPS</name>
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        <recommendedName>
            <fullName evidence="3">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4816979</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356329.2">
        <property type="nucleotide sequence ID" value="XM_001356293.4"/>
    </dbReference>
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        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816979"/>
    <dbReference type="InParanoid" id="A0A6I8UJ45"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356329.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="571" mass="64485" checksum="DC8348BA934F16E4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UKX6</accession>
    <name>A0A6I8UKX6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4817799</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357157.3">
        <property type="nucleotide sequence ID" value="XM_001357121.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UKX6"/>
    <dbReference type="KEGG" id="dpo:4817799"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
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        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="222"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
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        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001357157.3"/>
        </source>
    </evidence>
    <sequence length="554" mass="62222" checksum="2C991D565E80C0D5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL68</accession>
    <name>A0A6I8UL68_DROPS</name>
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        <submittedName>
            <fullName evidence="8 9">RNA polymerase-associated protein Rtf1-like isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="8 9" type="primary">LOC4818097</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8 9">MV-25-SWS-2005</strain>
            <tissue evidence="8 9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001357277.3">
        <property type="nucleotide sequence ID" value="XM_001357241.4"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015179852.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL68"/>
    <dbReference type="KEGG" id="dpo:4818097"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UL68">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Insect antifreeze protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016133">
        <property type="entry name" value="Insect_cyst_antifreeze_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115:SF8">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51156">
        <property type="entry name" value="Insect cysteine-rich antifreeze protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Plus3" evidence="6">
        <location>
            <begin position="272"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="17"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="467"/>
            <end position="496"/>
        </location>
    </feature>
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        <location>
            <begin position="510"/>
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        </location>
    </feature>
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            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
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        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001357277.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015035338.2"/>
        </source>
    </evidence>
    <sequence length="646" mass="74415" checksum="29E2E04285A81133" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN63</accession>
    <name>A0A6I8UN63_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4800879</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358074.3">
        <property type="nucleotide sequence ID" value="XM_001358037.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN63"/>
    <dbReference type="ESTHER" id="drops-q29ay8">
        <property type="family name" value="Acidic_Lipase"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800879"/>
    <dbReference type="InParanoid" id="A0A6I8UN63"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UN63">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000179">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026299488" description="Lipase" evidence="4">
        <location>
            <begin position="16"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001358074.3"/>
        </source>
    </evidence>
    <sequence length="378" mass="43095" checksum="0BFEAB5DFF537B23" modified="2020-08-12" version="1">MFIGLFLVYSWYTSGPEILASYNYPVEEHSVETTDNYILKLVHIPNSPNARNAQSPKPVVFMMHGMSGSSDSYLLIGPSDGLPYLLADAGFDVWLGNSRGNTYSRLHKYMDPKHKSFWNFSWHEMGTRDLPASIDYVLDRTSQRSLHYVGYSQGATQFLVMLSMRPEYNEKIKTSHLTAPAAFLRNMSTGLGSIVEKVILAFDDREWFSNRHGIPSWASIFCSVRPMKSICAALFMMVYGINGDQISKAIIMLILKTLPAGISSRQLKHYLQLKGSSRFCMYDHGKKTNRLIYGSSWPPDYPLKYVKPKSPINLYYSSSDFVVSEENVLLLAEKLSLCELHHIPYYSHIEFQFARAVGTTLNRPIVKLISKYETDRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UNS3</accession>
    <name>A0A6I8UNS3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cysteine-rich hydrophobic domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4801239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001358362.1">
        <property type="nucleotide sequence ID" value="XM_001358325.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UNS3"/>
    <dbReference type="FunCoup" id="A0A6I8UNS3">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="GeneID" id="4801239"/>
    <dbReference type="KEGG" id="dpo:4801239"/>
    <dbReference type="InParanoid" id="A0A6I8UNS3"/>
    <dbReference type="OMA" id="NYSMTEY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079242">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039735">
        <property type="entry name" value="CHIC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13005">
        <property type="entry name" value="CYSTEINE-RICH HYDROPHOBIC DOMAIN PROTEIN BRAIN X-LINKED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13005:SF4">
        <property type="entry name" value="CYSTEINE-RICH HYDROPHOBIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="3">
        <location>
            <begin position="48"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358362.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="19318" checksum="987EAEB39572923D" modified="2020-08-12" version="1">MSFSDFDAIYEDEQLEELEHFQDQSVAPVPEPIIIRGAGNMTVFGLSNRFNSEFPCGLLSRVAPEEFKATVGRINGVLKKSLPVNVKWLFCGCVCCCCTLGCSLWPVICLSKRTQLTLDKLFEWENNHLYHKLGLHWRLHKQQCDSNSMMEYVILIEFIPKTPIYRPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UP21</accession>
    <name>A0A6I8UP21_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4801552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358624.3">
        <property type="nucleotide sequence ID" value="XM_001358587.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UP21"/>
    <dbReference type="KEGG" id="dpo:4801552"/>
    <dbReference type="InParanoid" id="A0A6I8UP21"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UP21">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16021">
        <property type="entry name" value="ALP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000017">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004245">
        <property type="entry name" value="DUF229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974:SF9">
        <property type="entry name" value="DUF229 DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10974">
        <property type="entry name" value="FI08016P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02995">
        <property type="entry name" value="DUF229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001358624.3"/>
        </source>
    </evidence>
    <sequence length="710" mass="80900" checksum="F68CAF7A3388F569" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQT7</accession>
    <name>A0A6I8UQT7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylinositol 4-kinase type 2</fullName>
            <ecNumber evidence="9">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4802426</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="9"/>
    <dbReference type="RefSeq" id="XP_001359349.3">
        <property type="nucleotide sequence ID" value="XM_001359312.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQT7"/>
    <dbReference type="FunCoup" id="A0A6I8UQT7">
        <property type="interactions" value="1457"/>
    </dbReference>
    <dbReference type="GeneID" id="4802426"/>
    <dbReference type="KEGG" id="dpo:4802426"/>
    <dbReference type="InParanoid" id="A0A6I8UQT7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075543">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UQT7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="11">
        <location>
            <begin position="294"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="62"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="141"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="525"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="537"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359349.3"/>
        </source>
    </evidence>
    <sequence length="719" mass="79889" checksum="57B14FCFF12EC758" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR74</accession>
    <name>A0A6I8UR74_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Serine protease snake</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC4802767</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001359627.3">
        <property type="nucleotide sequence ID" value="XM_001359590.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR74"/>
    <dbReference type="FunCoup" id="A0A6I8UR74">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802767"/>
    <dbReference type="InParanoid" id="A0A6I8UR74"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000047">
        <property type="entry name" value="Trypsin eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF136">
        <property type="entry name" value="GH06673P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8 13" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026120399" evidence="10">
        <location>
            <begin position="20"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="147"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359627.3"/>
        </source>
    </evidence>
    <sequence length="397" mass="44901" checksum="4A0DAE4D3544072C" modified="2020-08-12" version="1">MFLSATLSLISVFVLCCHAQRPPPGPPPPPPHRVPRPQGPPPNRDAIYFPLERDILFPGSKGSNELEDKMWYHISDFHFEEVENLPQPKRRHHMSPPRPGQPFPPPPGGFKEKKNRRRRICEQKYSEYVERIFPNDTAVAEDANDADFDGRILARPGEYPHMAAVGFEADTGQIQYKCGGSLISENFVLTAAHCTSIYDTSPKWVRIGDLNLVADERTVVPQLMQIEDIFPHPNYSKELYYNDIALLKLQGEVELTVYVRPIRLWVFSELPTSIAFAMGYGSTSFAKPMTNRLTNLNLTVVPNAECNAELPPISETPNGVVDSQICAQDYILNRDTCQGDSGGPLQLNLPGRRRRQNIHYHLIGITSYGVFCRSSYPSVYTRISSFLDWIEGTTWNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URE1</accession>
    <name>A0A6I8URE1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Stabilizer of axonemal microtubules 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5" type="primary">LOC4802853</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4 5">MV-25-SWS-2005</strain>
            <strain evidence="2 3">MV2-25</strain>
            <tissue evidence="3 4 5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM154 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359688.2">
        <property type="nucleotide sequence ID" value="XM_001359651.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015038137.1">
        <property type="nucleotide sequence ID" value="XM_015182651.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237150.1">
        <property type="nucleotide sequence ID" value="XM_033381259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URE1"/>
    <dbReference type="GeneID" id="4802853"/>
    <dbReference type="KEGG" id="dpo:4802853"/>
    <dbReference type="OMA" id="KQPVPME"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080121">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033336">
        <property type="entry name" value="SAXO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31516:SF17">
        <property type="entry name" value="STABILIZER OF AXONEMAL MICROTUBULES 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001359688.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015038137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033237150.1"/>
        </source>
    </evidence>
    <sequence length="495" mass="54701" checksum="163704F65C05FA1D" modified="2020-08-12" version="1">MSGDCNTYCPNVCDPCQAPTDFTPCPPNSSCPPCDCGDYNGCCYQQPARVRPILPVSNFMRSTAPLDTDTIYRRSYYGNCGDNLRARPILPCSQIRTSTAPLEKCTVQKLSFMPPCPGQRTRPIRPMESGLRFEGPIYAMTSQKHDYVPKGVAKREPIKPAGAICTSSAPMERCTIQKLSYMPIDVCQNPPPKAVPQESHYCKPSGPLERCTIQKLSYMPVCLPAKEPTPWADKIRCVPPRYQNICTTYNLSYMPNCNPGRTAPVMPVSNLRFLGNDPSAGNTVYKLSYMPVDATRTKPTPILPRDTFCRPTGPLERCTIQKLSYQPNCAERTAPIRPMENGLRFDGPMYAMTTQKHDFVPKPHVRRSPIMPSSAFCRPSGAMERCTVNKLSYMPVDVTCFPRPEAVVPRAGFCRNEGPVENCTTYKLSYLPNCLPPKEPLPWARYTSYCRPSGPIEKCTIQKLSYGPPGAFSRCGGPCGNGAGDAGCFPKAGIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URE9</accession>
    <name>A0A6I8URE9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein Red</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">beag</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RED family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359515.2">
        <property type="nucleotide sequence ID" value="XM_001359478.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URE9"/>
    <dbReference type="FunCoup" id="A0A6I8URE9">
        <property type="interactions" value="2519"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802631"/>
    <dbReference type="InParanoid" id="A0A6I8URE9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039896">
        <property type="entry name" value="Red-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012492">
        <property type="entry name" value="RED_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012916">
        <property type="entry name" value="RED_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12765">
        <property type="entry name" value="RED PROTEIN IK FACTOR CYTOKINE IK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07807">
        <property type="entry name" value="RED_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07808">
        <property type="entry name" value="RED_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RED-like N-terminal" evidence="8">
        <location>
            <begin position="71"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Protein RED C-terminal" evidence="7">
        <location>
            <begin position="431"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="307"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="498"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="179"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="314"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="499"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="546"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001359515.2"/>
        </source>
    </evidence>
    <sequence length="558" mass="61492" checksum="E08A8DFA0929727F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USB3</accession>
    <name>A0A6I8USB3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ND-51L2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360108.4">
        <property type="nucleotide sequence ID" value="XM_001360071.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USB3"/>
    <dbReference type="FunCoup" id="A0A6I8USB3">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803358"/>
    <dbReference type="InParanoid" id="A0A6I8USB3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="10">
        <location>
            <begin position="371"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001360108.4"/>
        </source>
    </evidence>
    <sequence length="464" mass="52099" checksum="D4C7D9D89C9ACAA9" modified="2020-08-12" version="1">MFRKIIELNRLPGLTQWRRSFVAGKNDPPKSCLTNEKTTFGPLHDCDRVFQNLYGRHDWRLKGACKRGDWYHTAVLLEKGSDWIMEQVCQSGLRGRGGGGFFCGNKWNFLRCAESKQDKVLVMNCAEGEPGTCKDREIIRHEPHKIIEGCLLAGFAMGCNRAIIYVRNRFYNEACNLQFALAEAYRYGLLGPSACDTGIKFDILVQRGDRYLCGEETALVKCLMGKIGRPRRRPPFLTEQGYFSHPCVVINAESIAVLPTILRRGASWWADLGRSYNSGTKLFNISGQVNSPCTVEEEMSMPMRDLIEKHAGGVRGGWDNLLAIFPGGLSTPLINKQVAGEVLMDFECLEAAGSSLGSGALIVVNKQSDPLQVMLRSIQFYEKHTCKQCTYCRDGAIWLPELFDRFVKGEAHPHEIDWTLAIGKKIKDSNPICGLAQGQVNVAVSLVDNFRPYIEDRILQFIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTG3</accession>
    <name>A0A6I8UTG3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SmF</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360364.1">
        <property type="nucleotide sequence ID" value="XM_001360327.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTG3"/>
    <dbReference type="SMR" id="A0A6I8UTG3"/>
    <dbReference type="FunCoup" id="A0A6I8UTG3">
        <property type="interactions" value="1538"/>
    </dbReference>
    <dbReference type="GeneID" id="4803687"/>
    <dbReference type="KEGG" id="dpo:4803687"/>
    <dbReference type="InParanoid" id="A0A6I8UTG3"/>
    <dbReference type="OMA" id="GYMNVQL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074183">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000011">
        <property type="entry name" value="small nuclear ribonucleoprotein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 14" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="8"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001360364.1"/>
        </source>
    </evidence>
    <sequence length="88" mass="9739" checksum="198EF3AE27539880" modified="2020-08-12" version="1">MSAGMPINPKPFLNGLTGKPVLVKLKWGQEYKGFLVSVDGYMNMQLANTEEVIEGSVTGNLGEVLIRCNNVLYIKGMEDDDEEGEMRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UTK6</accession>
    <name>A0A6I8UTK6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Epoxide hydrolase</fullName>
            <ecNumber evidence="6">3.3.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Jheh3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes juvenile hormone hydrolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine + H2O = 2-{[(4-methoxybenzyl)(methyl)amino]methyl}-2-methylpropane-1,3-diol</text>
            <dbReference type="Rhea" id="RHEA:55764"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:139161"/>
            <dbReference type="ChEBI" id="CHEBI:139164"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cis-stilbene oxide + H2O = (1R,2R)-hydrobenzoin</text>
            <dbReference type="Rhea" id="RHEA:23900"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:50004"/>
            <dbReference type="ChEBI" id="CHEBI:50014"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.9" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001360678.2">
        <property type="nucleotide sequence ID" value="XM_001360641.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UTK6"/>
    <dbReference type="FunCoup" id="A0A6I8UTK6">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="ESTHER" id="drops-q290v8">
        <property type="family name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804057"/>
    <dbReference type="InParanoid" id="A0A6I8UTK6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="8">
        <location>
            <begin position="58"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360678.2"/>
        </source>
    </evidence>
    <sequence length="468" mass="53511" checksum="405B8FAB6ED78CE6" modified="2020-08-12" version="1">MKCLIVFGVIVAVFGAFVGYGYLVFSELTRPLPKPEFSDSTYWGPGEAKSYVPDKKIYEFRLDVPQAEIEDLRQQLNRTLRLAEPLDGISFEYGFNSYALDQFVEYWRDKYLTKWDERQELFNKFKQYTTEIQGLNIHFIREKASAEAAAKKHVYPLLLLHGWPGSVREFYDIIPMLTNDQNVTDYAFEVVAPSLVGYGWSDAATRPGFNAAEMATVMRNLMLRLGHKKFFIQGGDWGSIIGSNLATLYPENVIGYHSNMCVLNSPLAILKGIYGTYFSEKIIPSRFFYDHHFPVWDKYADLLRESGYFHIQATKPDTIGAALATSPVGLAAYILEKFQYCTNPGMQQDFGAIVTVFGLEPVLDNLMVYYLTNSITTSVRFYAENVSKAYRDLQLDRVPTPVPMGCSRFRYDLPSVTDWQLRDKFPNLKHSMYFQQGSHFAAMEMPAMLYQDFTAFVGKIGLHGEKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UU73</accession>
    <name>A0A6I8UU73_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription factor MafG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">maf-S</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. Maf subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001360993.1">
        <property type="nucleotide sequence ID" value="XM_001360956.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UU73"/>
    <dbReference type="SMR" id="A0A6I8UU73"/>
    <dbReference type="FunCoup" id="A0A6I8UU73">
        <property type="interactions" value="680"/>
    </dbReference>
    <dbReference type="GeneID" id="4804427"/>
    <dbReference type="KEGG" id="dpo:4804427"/>
    <dbReference type="InParanoid" id="A0A6I8UU73"/>
    <dbReference type="OMA" id="RSIKMDP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082130">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14717">
        <property type="entry name" value="bZIP_Maf_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000011">
        <property type="entry name" value="Transcription factor MafG, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF48">
        <property type="entry name" value="MAF-S, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Basic leucine zipper" evidence="8">
        <location>
            <begin position="23"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001360993.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="15688" checksum="0FE39219B729B5A3" modified="2020-08-12" version="1">MESKRERKSSLPPLSPCPISDITDDELVTISVRDLNRTLKMRGLNREEIVRMKQRRRTLKNRGYAASCRIKRIEQKDELETKKTSEWTELEQRHEDNELIRIEVGNWKNKYKALLHFAQQNDIPIPRELEGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUR3</accession>
    <name>A0A6I8UUR3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361210.2">
        <property type="nucleotide sequence ID" value="XM_001361173.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUR3"/>
    <dbReference type="GeneID" id="4804691"/>
    <dbReference type="KEGG" id="dpo:4804691"/>
    <dbReference type="InParanoid" id="A0A6I8UUR3"/>
    <dbReference type="OMA" id="QLRMREN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071903">
        <property type="expression patterns" value="Expressed in male reproductive system"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026353942" evidence="1">
        <location>
            <begin position="19"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361210.2"/>
        </source>
    </evidence>
    <sequence length="172" mass="20500" checksum="52BA125453C6425A" modified="2020-08-12" version="1">MNTLRFFILIAIGPIAICGNFEFNNVRCLVRDRLFLDFDYCYLRSVNRTYKYLSLKTKLHKLPITNTVTNAQVRMRENKRILYNFDVRFDACKFLRDRSNVIVNWLFQTFADFSNINHTCPYHHDIILDKLPVQHINNIIQTVVPDGRYYLNTTWFVDGIPRADVMIYFTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUZ7</accession>
    <name>A0A6I8UUZ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein immune deficiency</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">imd</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361314.2">
        <property type="nucleotide sequence ID" value="XM_001361277.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUZ7"/>
    <dbReference type="FunCoup" id="A0A6I8UUZ7">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804812"/>
    <dbReference type="InParanoid" id="A0A6I8UUZ7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000102">
        <property type="entry name" value="Imd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="177"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001361314.2"/>
        </source>
    </evidence>
    <sequence length="274" mass="29851" checksum="96B3984EB5BBB18F" modified="2020-08-12" version="1">MPKLKNLLPNIFGGSKEAPSMQSEGRLETDAAPVHDSEADNNNSGALALPSINTPTASADLTESVLRELSDPNYNSMDVVQSANIPGTGGDLSTVNTNNTMNVHSAQQQVVMNFSNASNLHFGSVYNFNQNLSASSSRKSSTGTIDELAVSPDGKRSNGTRKTVSIVAMMQSQDEPDPRLLDAVATHLGEGWKQVMRDLGHSEGQIDQAIIDHQMHGNIKEVIYQLLLQWVRSSESGVATVGRLTTLLWESQHRDCVQRLKLVWKALEKRKTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVK3</accession>
    <name>A0A6I8UVK3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4805140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361592.2">
        <property type="nucleotide sequence ID" value="XM_001361555.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVK3"/>
    <dbReference type="FunCoup" id="A0A6I8UVK3">
        <property type="interactions" value="1751"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805140"/>
    <dbReference type="InParanoid" id="A0A6I8UVK3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15750">
        <property type="entry name" value="FYVE_CARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16500">
        <property type="entry name" value="RING-HC_CARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000110">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF34 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051728">
        <property type="entry name" value="RING-FYVE_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055111">
        <property type="entry name" value="RNF34_RFFL_HeH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057299">
        <property type="entry name" value="RNF34_RFFL_SAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879">
        <property type="entry name" value="CASPASE REGULATOR, RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879:SF15">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RIFIFYLIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22968">
        <property type="entry name" value="RNF34L-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23632">
        <property type="entry name" value="SAP_RNF34_RFFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="648"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="136"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="259"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="352"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361592.2"/>
        </source>
    </evidence>
    <sequence length="695" mass="76445" checksum="B49D278A4AC5B61C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UVL3</accession>
    <name>A0A6I8UVL3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synaptogyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Syngr</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the synaptogyrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361603.1">
        <property type="nucleotide sequence ID" value="XM_001361566.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UVL3"/>
    <dbReference type="FunCoup" id="A0A6I8UVL3">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="GeneID" id="4805152"/>
    <dbReference type="KEGG" id="dpo:4805152"/>
    <dbReference type="InParanoid" id="A0A6I8UVL3"/>
    <dbReference type="OMA" id="FYLWSQW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070629">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016579">
        <property type="entry name" value="Synaptogyrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838">
        <property type="entry name" value="SYNAPTOGYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838:SF20">
        <property type="entry name" value="SYNAPTOGYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011282">
        <property type="entry name" value="Synaptogyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="30"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR011282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001361603.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="26599" checksum="B816EC58670B7C54" modified="2020-08-12" version="1">MDMLNQILSINNGGAYGGGKAGGAFDPLTFAMKPQVVIRALCWLFSVVVFGCISSEGWTEKEGKEYCLYNGDGMACKYGNMVGVFGFLASMGFMGGEFLFERMSSVKSRKRYVMADLGFSGLWAFMYFVAFLYLWSQWSSSPPPPLGIGAGSMQTAIWFCLFSIATWALCALMAYKRFLIGAGDEFTSAFETDPANVVHQQAYGYAMDNDNDQYSASPFGQGQQGGMEPQQQAGMEYQQPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UW68</accession>
    <name>A0A6I8UW68_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transducin beta-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4805428</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361829.2">
        <property type="nucleotide sequence ID" value="XM_001361792.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UW68"/>
    <dbReference type="FunCoup" id="A0A6I8UW68">
        <property type="interactions" value="2098"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4805428"/>
    <dbReference type="InParanoid" id="A0A6I8UW68"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013934">
        <property type="entry name" value="Utp13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08625">
        <property type="entry name" value="Utp13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="115"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="202"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="376"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="422"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="474"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="516"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="569"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 13 C-terminal" evidence="6">
        <location>
            <begin position="653"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001361829.2"/>
        </source>
    </evidence>
    <sequence length="787" mass="88583" checksum="C7EFCC9B5B3BC6A4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWE8</accession>
    <name>A0A6I8UWE8_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">U6 snRNA-associated Sm-like protein LSm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LSm1</name>
        <name evidence="11" type="synonym">LSM1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a role in the degradation of histone mRNAs, the only eukaryotic mRNAs that are not polyadenylated. Probably also part of an LSm subunits-containing complex involved in the general process of mRNA degradation.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Probably involved with other LSm subunits in the general process of degradation of mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with SLBP; interaction with SLBP occurs when histone mRNA is being rapidly degraded during the S phase. LSm subunits form a heteromer with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001361929.1">
        <property type="nucleotide sequence ID" value="XM_001361892.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWE8"/>
    <dbReference type="FunCoup" id="A0A6I8UWE8">
        <property type="interactions" value="1361"/>
    </dbReference>
    <dbReference type="GeneID" id="4805542"/>
    <dbReference type="KEGG" id="dpo:4805542"/>
    <dbReference type="CTD" id="27257"/>
    <dbReference type="InParanoid" id="A0A6I8UWE8"/>
    <dbReference type="OMA" id="IGYLRCV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078085">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01728">
        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000021">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001361929.1"/>
        </source>
    </evidence>
    <sequence length="137" mass="15768" checksum="3AA925EB8997477F" modified="2020-08-12" version="1">MDDLNPLAGTAHLLEEVDKKLMVLLRDGRTLIGYLRSVDQFANLVLQRTIERIHVGNQYGDIPRGVFIIRGENVVLLGEIDREKEQKLPLKEISVDEILDAQRREQEQRQEKHRLVSKALKERGLAVNAELINEDFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXU7</accession>
    <name>A0A6I8UXU7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHK kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132458.2">
        <property type="nucleotide sequence ID" value="XM_002132422.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXU7"/>
    <dbReference type="KEGG" id="dpo:6903591"/>
    <dbReference type="InParanoid" id="A0A6I8UXU7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXU7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015897">
        <property type="entry name" value="CHK_kinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004119">
        <property type="entry name" value="EcKL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012:SF59">
        <property type="entry name" value="CHK KINASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11012">
        <property type="entry name" value="PROTEIN KINASE-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02958">
        <property type="entry name" value="EcKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00587">
        <property type="entry name" value="CHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHK kinase-like" evidence="1">
        <location>
            <begin position="135"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132458.2"/>
        </source>
    </evidence>
    <sequence length="427" mass="49926" checksum="894F1B90B96C24C6" modified="2020-08-12" version="1">MSTDDYKPVLTRQNRLELFTLEECQEILVNLLADKQQHGVLKHFEIVPASEHVGFLGEYYHLELKYQLQDVKEEQTTRLFVKSVIFQNANMEYYMEKMGLIKKESKLYELLLNELKKFSPHVWCTKCYFTRNDLFVMQNVEDMGYVALPSGTRFLSEDQLGPILKTLATLHASSVAYERQQGQTIGVQLREWLKEVSVDPDVEWYTAGLRAVLAVAATHPDVRNDVRAQEYIAKELPRRLDSVYYMVNPSPVHRNVFVHRDAWGANVFYHKERPLEERSVLVDFQLCRYSPPAMDFHLVSYLNLEPANRKEMIGRLVNLYYETLAEELKTMGIDPSQEQLSREEFEQSLKDFALFGVTYNCIAATILRLPDNYLKSLKDQRPADFHRFCNIDRTPDVLSLMNDHQDFADYMYECVGDLLALTYHKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UXZ4</accession>
    <name>A0A6I8UXZ4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein ovarian tumor locus</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132950.2">
        <property type="nucleotide sequence ID" value="XM_002132914.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UXZ4"/>
    <dbReference type="KEGG" id="dpo:6903251"/>
    <dbReference type="InParanoid" id="A0A6I8UXZ4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UXZ4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF115">
        <property type="entry name" value="PROTEIN OVARIAN TUMOR LOCUS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="1">
        <location>
            <begin position="31"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132950.2"/>
        </source>
    </evidence>
    <sequence length="367" mass="42810" checksum="C41F47E61C1499D5" modified="2020-08-12" version="1">MTSMRPHTLKGPGNTLARRDPVDQYLANQHLYRKLMVRSDSSLFQVVAEQMYDTQNLHYEVRMDCVRFIMDKRRLFRRNITGNFNDYLSKLEKPNTRGTMIELRALCLMYGRNAIVHEPMRLGTPVTFSDKYKKNFSVFIDQYGNFDAVYTMGHIEEAAICQSITFKMLYQMCFKLPDVSLAAEKMLNPDTFYEGTTLEYSQNGTVVRILCRNGRSFDVDSPELTSCLLKDKQLCDFHNRVRWREYVQRFGRVPLSCTLKCVDDNIVPFSYSVAKSLDPNIYRNVELSSFLVMKKEAKKFQLCIRDDFNAGAHCLVQRHKWLGHMKKGFILKIDWKTSTFTVYLEETGTVLQVPPNAVHPLRPIYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V155</accession>
    <name>A0A6I8V155_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Cubilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">Cubn2</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="30">MV-25-SWS-2005</strain>
            <tissue evidence="30">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake. Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1. Acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the CBLIF-cobalamin complex. Ligand binding requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.</text>
    </comment>
    <comment type="subunit">
        <text evidence="24">Interacts with AMN. Component of the cubam complex composed of one CUBN trimer and one AMN chain. The cubam complex can dimerize. Interacts with LRP2 in a dual-receptor complex in a calcium-dependent manner. Found in a complex with PID1/PCLI1, LRP1 and CUBNI. Interacts with LRP1 and PID1/PCLI1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Lysosome membrane</location>
            <topology evidence="21">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135476.3">
        <property type="nucleotide sequence ID" value="XM_002135440.3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6900125"/>
    <dbReference type="InParanoid" id="A0A6I8V155"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V155">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000379">
        <property type="entry name" value="Cubilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000260">
        <property type="entry name" value="Notch receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000472">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>Arthropoda</taxon>
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            <taxon>Pterygota</taxon>
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            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
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            <taxon>Drosophilidae</taxon>
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    </evidence>
    <sequence length="509" mass="57267" checksum="3352388CE61B4D16" modified="2020-08-12" version="1">MDMDYMEAIASSSSGGGGSGSGSGSGSNISTSEATTAFSDGNATAENGGNASLAVGTTPDSTDYMSGDDEEMLRIAFFIGDFVHQYYIPILCCTGSIGNILSVFVFFKTKLRKLSSSFYLAALAVSDTCFLGGLFAQWLNFLNVDIYNKNYFCQFFTFFSYLASFCSVWFVVAFTVERFIAVIYPLKRQTMCTVRRAKIVLACLTLAGCLHCLPYILIAKPVFIPKLNATICDLNTEYKEQLALFNYWDSIVVYAVPFTTIAVLNTCTGCTVWKFATVRRTLTMHRMKPQTTSMPSSSTSPSAVASYRISASLKRQKSTGTHPSGQHSVARQQEHQQQEEHQHQHQHQQEHQQQHHHHINSCQHHCEITKKSDRRHKVQNSSQLKVTKMLLIVSTVFVCLNLPSCLLRIEAYWETESARNENSTIALQYIFHAFFITNFGINFVLYCVSGQNFRKAVLSIFRRVSAAQREAGNTQVTVSEYCRNTGTSTRRRMMTQHCWNEMHELHPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3I4</accession>
    <name>A0A6I8V3I4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycine-rich protein DOT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137801.2">
        <property type="nucleotide sequence ID" value="XM_002137765.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3I4"/>
    <dbReference type="KEGG" id="dpo:6897663"/>
    <dbReference type="InParanoid" id="A0A6I8V3I4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137801.2"/>
        </source>
    </evidence>
    <sequence length="76" mass="6186" checksum="2BE9C7F7CCDDD3BF" modified="2020-08-12" version="1">MGKNKGGGGGGGAGGGGGGNKKGGGAGGGSAGGAAAAAGAAGNKNKGGGAGGDKGGAKKGADQKPPPGAGKKGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3P0</accession>
    <name>A0A6I8V3P0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6897229</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137401.1">
        <property type="nucleotide sequence ID" value="XM_002137365.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3P0"/>
    <dbReference type="FunCoup" id="A0A6I8V3P0">
        <property type="interactions" value="1545"/>
    </dbReference>
    <dbReference type="GeneID" id="6897229"/>
    <dbReference type="KEGG" id="dpo:6897229"/>
    <dbReference type="InParanoid" id="A0A6I8V3P0"/>
    <dbReference type="OMA" id="MIANNCP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247949">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_eL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_eL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002137401.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12279" checksum="CA4BCEF8ACEEE01F" modified="2020-08-12" version="1">MVAPKKQKKALESTNARLALVMKSGKYCLGYKQSLKTLRQGKAKLVLIASNTPALRKSEIEYYAMLAKTEVQHYSGTNIELGTACGKYFRVCTMSITDPGDSDIIRSLTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3P9</accession>
    <name>A0A6I8V3P9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="12">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC6897833</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_002137938.3">
        <property type="nucleotide sequence ID" value="XM_002137902.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3P9"/>
    <dbReference type="FunCoup" id="A0A6I8V3P9">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897833"/>
    <dbReference type="InParanoid" id="A0A6I8V3P9"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="518"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="595"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="624"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="649"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="673"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="780"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="820"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="854"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="885"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="13">
        <location>
            <begin position="444"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002137938.3"/>
        </source>
    </evidence>
    <sequence length="940" mass="105647" checksum="03C22D422F59C5BA" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3U7</accession>
    <name>A0A6I8V3U7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphoriboisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Rpi</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="RefSeq" id="XP_002138261.2">
        <property type="nucleotide sequence ID" value="XM_002138225.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3U7"/>
    <dbReference type="FunCoup" id="A0A6I8V3U7">
        <property type="interactions" value="2001"/>
    </dbReference>
    <dbReference type="GeneID" id="6898192"/>
    <dbReference type="KEGG" id="dpo:6898192"/>
    <dbReference type="InParanoid" id="A0A6I8V3U7"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245882">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000018">
        <property type="entry name" value="Ribose-5-phosphate isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000001">
        <property type="entry name" value="Ribose-5-phosphate isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002138261.2"/>
        </source>
    </evidence>
    <sequence length="275" mass="30290" checksum="F63AE78EC3891B46" modified="2020-08-12" version="1">MLLGLLRRGLSQPHFNQHFLNAQQLLLGAQTRNMGDEISLDEAKKKAARTAVDEWVKEDTKIVGIGSGSTVVYAVQRIAERVWKDGELTDLICVPSSYQAQHLILDYNLNLGSVDRNPIIDVAIDGADEVDSNMVLIKGGGGCLLQEKVVAACSKKFIVVADYTKKSLHLGEQWCRGVPIEVAPMAQVPIKLQIEAMFGGEVVLRMAKVKAGPIITDNGNFLLDWKFVGKREYNWDEVNRTITMIPGVLETGLFVDMAHKCYFGMADGSVKAQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V416</accession>
    <name>A0A6I8V416_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6897485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137634.3">
        <property type="nucleotide sequence ID" value="XM_002137598.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V416"/>
    <dbReference type="FunCoup" id="A0A6I8V416">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897485"/>
    <dbReference type="InParanoid" id="A0A6I8V416"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006631">
        <property type="entry name" value="DM4_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21253">
        <property type="entry name" value="F-BOX ONLY PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21253:SF0">
        <property type="entry name" value="F-BOX ONLY PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07841">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00718">
        <property type="entry name" value="DM4_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026160517" evidence="1">
        <location>
            <begin position="17"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137634.3"/>
        </source>
    </evidence>
    <sequence length="278" mass="32896" checksum="7C133CFEAAF2EECA" modified="2020-08-12" version="1">MRCVTLVLLYLHTVWAALRNQTELSDKSVALQDVLLSRRVRGLVFPDKASLLLTAALTKIIVGGRPSGLQYSLEFDMYIPLPDTVEGWQPKILKRLRPKPSTQRRWDWYDNQGWAKYTDIPHTPIHRTVPYSSSHLDANSFYQTPWHLSTTTPWKMHRQAQYDEEAYESWSHVPNWKLHRGYRERRAIFDQLEDMGRVFQLDIRSCIKRAMCELRSRLNGGFLMEDLMRIILTVPDEVTDGKYRHRMDIRDCAHFYALSCPYRVLDFLTQNMNKYRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V471</accession>
    <name>A0A6I8V471_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lactoylglutathione lyase</fullName>
            <ecNumber evidence="3">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Aldoketomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ketone-aldehyde mutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Methylglyoxalase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">S-D-lactoylglutathione methylglyoxal lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Glo1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glyoxalase I family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002138496.1">
        <property type="nucleotide sequence ID" value="XM_002138460.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V471"/>
    <dbReference type="FunCoup" id="A0A6I8V471">
        <property type="interactions" value="1139"/>
    </dbReference>
    <dbReference type="GeneID" id="6898453"/>
    <dbReference type="KEGG" id="dpo:6898453"/>
    <dbReference type="InParanoid" id="A0A6I8V471"/>
    <dbReference type="OMA" id="THNWDTP"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00675"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246192">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="13">
        <location>
            <begin position="27"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_002138496.1"/>
        </source>
    </evidence>
    <sequence length="178" mass="20407" checksum="96EDD3950704DCC1" modified="2020-08-12" version="1">MGDVTGISNAEAEALCKKPDKATEEFLFQQTMYRIKDPRKSLPFYTGVLGMTLLAKLDFPESMFSLYFMGYESADDIPKDPKERRSWALSRKATIELTHNWGTENDLKQIYHNGNSEPRGFGHIGLMVPDVYAACKRFEEQGVEFVKKPDDGRMKGLAFIKDPDGYWIEIFNAYTVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4U5</accession>
    <name>A0A6I8V4U5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transmembrane protease serine 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC6898861</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002138833.3">
        <property type="nucleotide sequence ID" value="XM_002138797.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4U5"/>
    <dbReference type="KEGG" id="dpo:6898861"/>
    <dbReference type="InParanoid" id="A0A6I8V4U5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000146">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026184586" evidence="11">
        <location>
            <begin position="23"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="54"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="356"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_002138833.3"/>
        </source>
    </evidence>
    <sequence length="597" mass="66062" checksum="8156F9C236DA7C0C" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4U9</accession>
    <name>A0A6I8V4U9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable ribonuclease ZC3H12C isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6898965</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002138925.3">
        <property type="nucleotide sequence ID" value="XM_002138889.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4U9"/>
    <dbReference type="FunCoup" id="A0A6I8V4U9">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898965"/>
    <dbReference type="InParanoid" id="A0A6I8V4U9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V4U9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18719">
        <property type="entry name" value="PIN_Zc3h12a-N4BP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000001">
        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF35">
        <property type="entry name" value="LD08718P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase NYN" evidence="2">
        <location>
            <begin position="373"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002138925.3"/>
        </source>
    </evidence>
    <sequence length="527" mass="60175" checksum="76C4D338581E7279" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4Y4</accession>
    <name>A0A6I8V4Y4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">trypsin</fullName>
            <ecNumber evidence="11">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC6898786</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="11"/>
    <dbReference type="RefSeq" id="XP_002138763.2">
        <property type="nucleotide sequence ID" value="XM_002138727.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4Y4"/>
    <dbReference type="SMR" id="A0A6I8V4Y4"/>
    <dbReference type="KEGG" id="dpo:6898786"/>
    <dbReference type="InParanoid" id="A0A6I8V4Y4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050430">
        <property type="entry name" value="Peptidase_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276:SF91">
        <property type="entry name" value="AT26814P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24276">
        <property type="entry name" value="POLYSERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026236698" description="trypsin" evidence="13">
        <location>
            <begin position="17"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="31"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_002138763.2"/>
        </source>
    </evidence>
    <sequence length="256" mass="25794" checksum="F0018EADDD8F9102" modified="2020-08-12" version="1">MLKFVILLSAVACALGGTIPEGLLPQLDGRIVGGTATTISSFPWQISLQRSGSHSCGGSVYSASIVVTAAHCLQSVSASTLQVRAGSTYWSSGGTVVKVASFKNHEGYNANTMVNDIAVIRLSSALGLSSTIKAIGLASSAPANGAAASVSGWGTLSYGSSSIPTTLQYVNVNIVSQSQCASSTYGYGSEIKSTMICAYSSGKDACQGDSGGPLVSGGVLVGVVSWGYGCAYANYPGVYADVAALRSWVVSAASSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5Z5</accession>
    <name>A0A6I8V5Z5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4802561</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736762.1">
        <property type="nucleotide sequence ID" value="XM_003736714.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5Z5"/>
    <dbReference type="FunCoup" id="A0A6I8V5Z5">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="GeneID" id="4802561"/>
    <dbReference type="InParanoid" id="A0A6I8V5Z5"/>
    <dbReference type="OMA" id="QFALAYN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0073352">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V5Z5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="7"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_003736762.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="19980" checksum="E8402C5612F5E671" modified="2020-08-12" version="1">MPDVKFLAVLPDNSNASVTKLTIKGTLLQQPQQFSVNFVNSPVCTDNITYHFKVVIPTQTIIENYKRNGEWSSLQQEHYLNIFDGTGGSDKTLTQSFASIMNEIDEAFQLSSTESTFRLEFTFDYDNSTMIVYQVRETGHNFISKYETLFSLSEIQAIQVWGDVQKVNQFALSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V669</accession>
    <name>A0A6I8V669_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Mf isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Mf</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_003736725.3">
        <property type="nucleotide sequence ID" value="XM_003736677.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V669"/>
    <dbReference type="FunCoup" id="A0A6I8V669">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V669"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V669">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031828">
        <property type="entry name" value="Myofilin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15929">
        <property type="entry name" value="Myofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_003736725.3"/>
        </source>
    </evidence>
    <sequence length="366" mass="41744" checksum="5C5E8B3434C4FA8F" modified="2020-08-12" version="1">MFKNHLEMIGRNESPSKKAKFWQSYIRSLKGSEDIRAHETPRASRPYSAYLDNPTYRSIYDEPATANERVQSSGYRYLPVSRDTYGYSPRAIYDHHYSRTIPANYDAEKAWNDHLKRMQEIERRYPSRYGLYLRDKPLTPNSLVPLEYEPEDKLLAELNKARRSASPFRPTRSTRAGSEPYVPEPVYWNREGSVPRRGPSVFDRATSLAPFTRPSFRASSLEPLDELFESKLPAIAEAGAVAAEAAAATRHLGIFERAGSPSPTPVKAGRWGPRSTEVAYDAEGLPIFHPRNRFRDLLSPSPNLPISSIVRDPFWWDVDDLVPFRATSVPRGSSPVARDSYLSPVKNRYLWSKHPARPLTPHRSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V9T4</accession>
    <name>A0A6I8V9T4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Accessory gland-specific peptide 70A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC26531953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015036782.2">
        <property type="nucleotide sequence ID" value="XM_015181296.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V9T4"/>
    <dbReference type="KEGG" id="dpo:26531953"/>
    <dbReference type="InParanoid" id="A0A6I8V9T4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046008">
        <property type="term" value="P:regulation of female receptivity, post-mating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012608">
        <property type="entry name" value="Sex_peptide"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08138">
        <property type="entry name" value="Sex_peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0085">Behavior</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026304876" evidence="6">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015036782.2"/>
        </source>
    </evidence>
    <sequence length="57" mass="6250" checksum="365177AA4F916A54" modified="2020-08-12" version="1">MKVATSAMLLLMLVEAAVGVPAWGRMTSRKPTPKQSQAQFQKWCRLNFGPAWGGRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VAD0</accession>
    <name>A0A6I8VAD0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongator complex protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">Elp6</name>
        <name evidence="5" type="synonym">LOC4801273</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <strain evidence="5">MV2-25</strain>
            <tissue evidence="5 6">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358393.1">
        <property type="nucleotide sequence ID" value="XM_001358356.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015037122.1">
        <property type="nucleotide sequence ID" value="XM_015181636.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VAD0"/>
    <dbReference type="GeneID" id="4801273"/>
    <dbReference type="KEGG" id="dpo:4801273"/>
    <dbReference type="OMA" id="NVKIFNP"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0082047">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19495">
        <property type="entry name" value="Elp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002506">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018627">
        <property type="entry name" value="ELP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184:SF6">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09807">
        <property type="entry name" value="ELP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015037122.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="27971" checksum="8A8333ED47F7C037" modified="2020-08-12" version="1">MATSVLLACGLNEQKLPGFVHISEESNVDGSFLISCILGQRLRISNAGTLLVCLQHHYQHYFNAGMRLGYNTNIFQGKTLNVIDVLSDMASEGLSCKWLKNPDGLTLTEQLVEDIRAQVESNYANRNSYTILIDNLSILFNLGASKLQVQQFCQDLASLAKERDNLTVITKLSNSDIYQPTDNNVAKLGQVRIQVVRLKSGVFREVDGKLLIERVLDEGSYACEEARKEVLYKVNDRNVKVFTPGEVGVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VEZ5</accession>
    <name>A0A6I8VEZ5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="11">HMG-CoA synthase</shortName>
            <ecNumber evidence="3 11">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 16 17 18" type="primary">Hmgs</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 17">MV-25-SWS-2005</strain>
            <strain evidence="14">MV2-25</strain>
            <tissue evidence="15 17">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="16 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 18">MV-25-SWS-2005</strain>
            <strain evidence="14">MV2-25</strain>
            <tissue evidence="16 18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="function">
        <text evidence="8">This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:10189"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="3 11"/>
    <dbReference type="RefSeq" id="XP_015039366.2">
        <property type="nucleotide sequence ID" value="XM_015183880.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039367.2">
        <property type="nucleotide sequence ID" value="XM_015183881.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234350.1">
        <property type="nucleotide sequence ID" value="XM_033378459.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234351.1">
        <property type="nucleotide sequence ID" value="XM_033378460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VEZ5"/>
    <dbReference type="KEGG" id="dpo:4803801"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00102"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="6 11" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="13">
        <location>
            <begin position="179"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="9">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_015039366.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_015039367.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033234350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033234351.1"/>
        </source>
    </evidence>
    <sequence length="465" mass="51073" checksum="CC3DB5E6F6DE17C0" modified="2020-08-12" version="1">MATNWPENVGIRAIEVLFPSQYVDQTELEQFDGASAGKYTIGLGQAKMGFCSDREDVNSLCLTVVSRLLERHHIKHTEIGRLEVGTETIVDKSKSVKSVLMQLFADSGNTDIEGIDTTNACYGGTAALFNAINWIESSSWDGRYALAVCADIAVYAKGAARPTGGAGAVAMLVGPNAPLVFERGLRATHMEHAYDFYKPDLSSEYPTVDGKLSIQCYLSALDTCYRLYRQKFEKQQAKTQGGKVGLDNFDAILFHTPFCKLVQKSVGRLSFNDFLLTSEGKRAEQFVGLERFNNATLEGTYFDRDVEKAFLTQSADVFEAKTKKTLLLANQVGNMYTPSVYSGLVSLLIGVPAADLVGKRIGVFSYGSGLAASMYSISVTQDAAAFEKFVAQLDYVLPLLNSREKVAPEQFSELMEVREKNNHAAPYTPTGSVGVLFPGTYYLKDVDSLHRRTYERTPTISNGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VF49</accession>
    <name>A0A6I8VF49_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein Rcd6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">Rcd6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <strain evidence="3">MV2-25</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015039945.1">
        <property type="nucleotide sequence ID" value="XM_015184459.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234114.1">
        <property type="nucleotide sequence ID" value="XM_033378223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VF49"/>
    <dbReference type="GeneID" id="4804430"/>
    <dbReference type="OMA" id="QQYTAYN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070873">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VF49">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_015039945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033234114.1"/>
        </source>
    </evidence>
    <sequence length="524" mass="57974" checksum="DAC47B75DC5CB1F1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGC4</accession>
    <name>A0A6I8VGC4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein O-mannosyl-transferase TMTC3</fullName>
            <ecNumber evidence="6">2.4.1.109</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Transmembrane O-mannosyltransferase targeting cadherins 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Transmembrane and tetratricopeptide repeat-containing 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24 25 26" type="primary">Tmtc3</name>
    </gene>
    <organism evidence="23 25">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">MV-25-SWS-2005</strain>
            <strain evidence="23">MV2-25</strain>
            <tissue evidence="24">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="25 26" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="25 26">MV-25-SWS-2005</strain>
            <tissue evidence="25 26">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers mannosyl residues to the hydroxyl group of serine or threonine residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMTC family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001361920.3">
        <property type="nucleotide sequence ID" value="XM_001361883.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040704.2">
        <property type="nucleotide sequence ID" value="XM_015185218.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033233258.1">
        <property type="nucleotide sequence ID" value="XM_033377367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VGC4"/>
    <dbReference type="KEGG" id="dpo:4805532"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000175">
        <property type="entry name" value="transmembrane and TPR repeat-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000239">
        <property type="entry name" value="Transmembrane and TPR repeat-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000528">
        <property type="entry name" value="Transmembrane and TPR repeat-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013618">
        <property type="entry name" value="TMTC_DUF1736"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44395">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44395:SF1">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08409">
        <property type="entry name" value="TMTC_DUF1736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 20" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1736" evidence="22">
        <location>
            <begin position="330"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="20">
        <location>
            <begin position="520"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="20">
        <location>
            <begin position="637"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="20">
        <location>
            <begin position="671"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="20">
        <location>
            <begin position="742"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00075262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_001361920.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_015040704.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_033233258.1"/>
        </source>
    </evidence>
    <sequence length="933" mass="105930" checksum="F2B724086792B4DC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VGD4</accession>
    <name>A0A6I8VGD4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">F-box only protein 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7 8 9" type="primary">dmpd</name>
    </gene>
    <organism evidence="4 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5 6 7">MV-25-SWS-2005</strain>
            <strain evidence="4">MV2-25</strain>
            <tissue evidence="5 6 7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361363.2">
        <property type="nucleotide sequence ID" value="XM_001361326.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040249.2">
        <property type="nucleotide sequence ID" value="XM_015184763.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040250.2">
        <property type="nucleotide sequence ID" value="XM_015184764.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015040251.2">
        <property type="nucleotide sequence ID" value="XM_015184765.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015184766.2"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="F-box_FBXO28"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039719">
        <property type="entry name" value="FBXO28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13252:SF1">
        <property type="entry name" value="DAMPENED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13252">
        <property type="entry name" value="F-BOX ONLY PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
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        <location>
            <begin position="93"/>
            <end position="142"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
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        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
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        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50181"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001361363.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015040249.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015040250.2"/>
        </source>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015040251.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_015040252.2"/>
        </source>
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    <sequence length="579" mass="63562" checksum="F04F03586906F338" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VI15</accession>
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        <submittedName>
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        <name evidence="7" type="primary">LOC6901334</name>
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            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="7">MV-25-SWS-2005</strain>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="58"/>
            <end position="75"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_015041581.2"/>
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    </evidence>
    <sequence length="274" mass="32224" checksum="DB92E667CAFC128D" modified="2020-08-12" version="1">MNKNISQFLYPFLWIGITFVYYIVYKIRLIHVDKRFLVYAVDMIYILCLMPHRLDWMVPYCLLLLMISHWTHWFARQQVEPLDVKYTPFNSLIVLVVLDGLLILSYRCMLDDGSLKDRLTIEQSLLMVHAVHTTRIWSACKALKVECLYCDQLIPETMIFFEGFQLVGTIFILFYYWPAVGLGNVLYRLQCFYTVLDLIGSRKDARSAIEKKCKKLHYSEIMDLFCIICRESMEPTEQCGWVRLPCGHSYHRCCAMARFNGQFLCPLCGVKIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VID3</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="primary">LOC6901331</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041574.2">
        <property type="nucleotide sequence ID" value="XM_015186088.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="dpo:6901331"/>
    <dbReference type="InParanoid" id="A0A6I8VID3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015041574.2"/>
        </source>
    </evidence>
    <sequence length="153" mass="17075" checksum="8DFD24A94DC25890" modified="2020-08-12" version="1">MDSYILIREQSIAKKQKEGGQELVADMNKNLDSLPLAQTQSSWPSFLHHSLPDGSKSDKLHEAAKEFDAALKENITVDNYIKPLYSSLIERANRTSKIESATPLRNTLIKSVVFEAKGNDLLAQKLQLTGAEIPSRTLCPAKMLEMFEILSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VMW5</accession>
    <name>A0A6I8VMW5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6897488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033232401.1">
        <property type="nucleotide sequence ID" value="XM_033376510.1"/>
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    <dbReference type="KEGG" id="dpo:6897488"/>
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    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038599">
        <property type="entry name" value="LAP1C-like_C_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033232401.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="13181" checksum="E689794DACE08BB7" modified="2020-08-12" version="1">MHMNIDDFAPPTEDYGFGIAQFQKKLKDGKVFRIVSLNEIPPSSARALLTICDTYSPIAADAVIFLTLQTFNTTDSGNSVELAWKTLFELWGTHLADNELDPLITRVTDQVLHQKGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VNT6</accession>
    <name>A0A6I8VNT6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nicastrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4800251</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nicastrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033232528.1">
        <property type="nucleotide sequence ID" value="XM_033376637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VNT6"/>
    <dbReference type="FunCoup" id="A0A6I8VNT6">
        <property type="interactions" value="2110"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800251"/>
    <dbReference type="InParanoid" id="A0A6I8VNT6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007220">
        <property type="term" value="P:Notch receptor processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03881">
        <property type="entry name" value="M28_Nicastrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000127">
        <property type="entry name" value="Nicastrin, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041084">
        <property type="entry name" value="Ncstrn_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008710">
        <property type="entry name" value="Nicastrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21092:SF0">
        <property type="entry name" value="NICASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18266">
        <property type="entry name" value="Ncstrn_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05450">
        <property type="entry name" value="Nicastrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026160835" description="Nicastrin" evidence="11">
        <location>
            <begin position="25"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="660"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="Nicastrin small lobe" evidence="12">
        <location>
            <begin position="40"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033232528.1"/>
        </source>
    </evidence>
    <sequence length="700" mass="78079" checksum="641C9170C7190B25" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQZ4</accession>
    <name>A0A6I8VQZ4_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="3 7">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ald1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 7">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="3 7"/>
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        <property type="nucleotide sequence ID" value="XM_033377571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQZ4"/>
    <dbReference type="KEGG" id="dpo:4802184"/>
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        <property type="reaction ID" value="UER00183"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00948">
        <property type="entry name" value="FBP_aldolase_I_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000021">
        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033379">
        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ALDOLASE_CLASS_I"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033233462.1"/>
        </source>
    </evidence>
    <sequence length="363" mass="39310" checksum="2396A23738A35847" modified="2020-08-12" version="1">MTTYFNYPSKELQDELRGIAQAIVAPGKGILAADESVSTMGKRLQDIGVENSDENRRAYRQLLFSTDPKLAENISGVILFHETLYQKADDGTPFVDILKKKGIIPGIKVDKGVVPLFGSEDEVTTQGLDDLAARCAQYKKDGCDFAKWRCVLKIGKNTPSYQSILENANVLARYASICQSQRIVPIVEPEVLPDGDHDLDRAQKVTETVLAAVYKALSDHHVYLEGTLLKPNMVTAGQSAKKNTPQEIGLATVLALRRTVPAAVTGVTFLSGGQSEEEATVNLSAINNVPLGRPWALTFSYGRALQASVLKAWGGKKENIAAGQNELLKRARANALACQGKYVAGSIPSYAANTSLFIAGHKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VS83</accession>
    <name>A0A6I8VS83_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine-enriched protein isoform X4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gprs</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033233925.1">
        <property type="nucleotide sequence ID" value="XM_033378034.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8VS83"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd18507">
        <property type="entry name" value="BACK_GPRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18294">
        <property type="entry name" value="BTB_POZ_BTBD19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051481">
        <property type="entry name" value="BTB-POZ/Galectin-3-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410">
        <property type="entry name" value="HL07962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410:SF46">
        <property type="entry name" value="SERINE-ENRICHED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="40"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="530"/>
        </location>
    </feature>
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        <location>
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            <end position="618"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="711"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="877"/>
        </location>
    </feature>
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        <location>
            <begin position="927"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1005"/>
        </location>
    </feature>
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        <location>
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            <end position="1097"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1203"/>
            <end position="1224"/>
        </location>
    </feature>
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        <location>
            <begin position="1241"/>
            <end position="1267"/>
        </location>
    </feature>
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        <location>
            <begin position="336"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="381"/>
        </location>
    </feature>
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        <location>
            <begin position="403"/>
            <end position="415"/>
        </location>
    </feature>
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            <begin position="431"/>
            <end position="454"/>
        </location>
    </feature>
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        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
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        <location>
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            <end position="611"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="857"/>
            <end position="876"/>
        </location>
    </feature>
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        <location>
            <begin position="1125"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1203"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1247"/>
            <end position="1267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033233925.1"/>
        </source>
    </evidence>
    <sequence length="1317" mass="145767" checksum="3C7F8911B0719196" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VT10</accession>
    <name>A0A6I8VT10_DROPS</name>
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        <submittedName>
            <fullName evidence="4">AF4/FMR2 family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4803986</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033234197.1">
        <property type="nucleotide sequence ID" value="XM_033378306.1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="InParanoid" id="A0A6I8VT10"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:nuclear body"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026095">
        <property type="entry name" value="Myb/SANT-like_DNA-bd_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22666">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22666:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="96"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="734"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
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        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="694"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033234197.1"/>
        </source>
    </evidence>
    <sequence length="734" mass="79059" checksum="893B297CCEF46821" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VV40</accession>
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        <submittedName>
            <fullName evidence="9 10 11">Uncharacterized protein pwn isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12 13 14" type="primary">pwn</name>
    </gene>
    <organism evidence="8 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11 12">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="10 11 12">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="9 13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9 13 14">MV-25-SWS-2005</strain>
            <strain evidence="8">MV2-25</strain>
            <tissue evidence="9 13 14">Whole body</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_001360167.4"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_015183613.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015039102.2">
        <property type="nucleotide sequence ID" value="XM_015183616.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234950.1">
        <property type="nucleotide sequence ID" value="XM_033379059.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_033379060.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033234952.1">
        <property type="nucleotide sequence ID" value="XM_033379061.1"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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            <begin position="1140"/>
            <end position="1165"/>
        </location>
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        </location>
    </feature>
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            <begin position="1100"/>
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        <location>
            <begin position="1"/>
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        </location>
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    </feature>
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        <location>
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    </feature>
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        <location>
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        </location>
    </feature>
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            <begin position="1375"/>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001360204.3"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015039099.2"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015039102.2"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033234950.1"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033234951.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_033234952.1"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W127</accession>
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        <recommendedName>
            <fullName evidence="13">Venom dipeptidyl peptidase 4</fullName>
        </recommendedName>
    </protein>
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        <name evidence="19 20" type="primary">Dpp10</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19 20">MV-25-SWS-2005</strain>
            <tissue evidence="19 20">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endomembrane system</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015036961.2">
        <property type="nucleotide sequence ID" value="XM_015181475.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033237032.1">
        <property type="nucleotide sequence ID" value="XM_033381141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W127"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W127">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="17">
        <location>
            <begin position="123"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="16">
        <location>
            <begin position="718"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="273"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="535"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="609"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="609"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_015036961.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033237032.1"/>
        </source>
    </evidence>
    <sequence length="947" mass="106595" checksum="B4C92C9C1BCC4179" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W4V8</accession>
    <name>A0A6I8W4V8_DROPS</name>
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        <submittedName>
            <fullName evidence="6 7 8">Pneumococcal serine-rich repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8 9 10 11" type="primary">RhoGEF64C</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6 7 8">MV-25-SWS-2005</strain>
            <tissue evidence="6 7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033238385.1">
        <property type="nucleotide sequence ID" value="XM_033382494.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238386.1">
        <property type="nucleotide sequence ID" value="XM_033382495.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238387.1">
        <property type="nucleotide sequence ID" value="XM_033382496.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238389.1">
        <property type="nucleotide sequence ID" value="XM_033382498.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238390.1">
        <property type="nucleotide sequence ID" value="XM_033382499.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033238391.1">
        <property type="nucleotide sequence ID" value="XM_033382500.1"/>
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    <dbReference type="KEGG" id="dpo:6900063"/>
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        <property type="component" value="Chromosome X"/>
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    <dbReference type="ExpressionAtlas" id="A0A6I8W4V8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000038">
        <property type="entry name" value="Myosin-M heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051480">
        <property type="entry name" value="Endocytic_GEF_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006:SF5">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="1792"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="457"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="609"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="657"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="873"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1057"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1298"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1326"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1486"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1693"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="197"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="260"/>
            <end position="271"/>
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    </feature>
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        <location>
            <begin position="291"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="355"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="505"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="609"/>
            <end position="625"/>
        </location>
    </feature>
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        <location>
            <begin position="628"/>
            <end position="637"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="657"/>
            <end position="676"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="873"/>
            <end position="891"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="909"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="937"/>
            <end position="954"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="963"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="984"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1028"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1057"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1071"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1298"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1486"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1715"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1737"/>
            <end position="1749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033238385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033238386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033238387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033238389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033238390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238391.1"/>
        </source>
    </evidence>
    <sequence length="2187" mass="235439" checksum="01DBEB1E63251ED9" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6A6</accession>
    <name>A0A6I8W6A6_DROPS</name>
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        <submittedName>
            <fullName evidence="11 12">cAMP-dependent protein kinase catalytic subunit 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">Pka-C3</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11 12">MV-25-SWS-2005</strain>
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        <property type="nucleotide sequence ID" value="XM_033382995.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:imaginal disc-derived wing morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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            <begin position="211"/>
            <end position="465"/>
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            <begin position="11"/>
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            <begin position="144"/>
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    </feature>
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        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="240"/>
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        <ligand>
            <name>ATP</name>
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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        <source>
            <dbReference type="PROSITE" id="PS51285"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_015042850.2"/>
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        <source>
            <dbReference type="RefSeq" id="XP_033238886.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="58047" checksum="0478BA0836870B0E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W752</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein isoform X1</fullName>
        </submittedName>
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        <name evidence="3" type="primary">LOC117184688</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_033239195.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13013" checksum="3014889E75AEB6DA" modified="2020-08-12" version="1">MGKLLHLNLNHCRTAQDLMTHTVLKQQINVVLLSGPYKGTNLWKRSHRASRASLHAARISGDHQRSQIPGHNSGGLQRLGHDLGTPRGTALLDEFTSLNVCLLNIGSIPTYNNAGRESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7A4</accession>
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        <submittedName>
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        <name evidence="6 7" type="primary">mri</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_033383342.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR039885">
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    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21637:SF0">
        <property type="entry name" value="AT10158P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21637">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16017">
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    </dbReference>
    <dbReference type="SMART" id="SM00225">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="118"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033239232.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_033239233.1"/>
        </source>
    </evidence>
    <sequence length="453" mass="49805" checksum="FB9585BC600B760D" modified="2020-08-12" version="1">MSTTPNSNSSSSNSNNTSVNKQPQQQQHQHQHQLQQQDTYSSDSSSIEENSDEAAEERRRRISKNRSRLQRPLNKSQHTMSSGGAVPKHDGKSPGGASTSGQAAASGGAVGRLVVPPERISMLVDGVRFTVEQSLLTAHPTTMLGTMFGSGFQFVHPNERGEYDVADGISHLVFRAILEYYKSGVIRCPPTVSVPELKEACDYLLIPFDATTVRCQNLRGLLHELSNEGARQQFELFLEDLILPLMVASAQRGDRECHVVVLLEDDMVDWDEEFPPQMGEEYCQTVHSTAMHRFFKYIENRDVAKQVMKDRGLKKIRCGIEGYPTHKEKIRRRPGGRAEVIYSYVQRPFIHMSWEKEEAKSRHVDFQCVKSKSVTNLAEANADPPLELDASGNPIPPIAVVNPNPNNAELAPVPAVGAAAVVAVDEAAGGVVMLNDLEQAVAAVAAGIVDENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8F9</accession>
    <name>A0A6I8W8F9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibrous sheath CABYR-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239567.1">
        <property type="nucleotide sequence ID" value="XM_033383676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W8F9"/>
    <dbReference type="KEGG" id="dpo:6900069"/>
    <dbReference type="InParanoid" id="A0A6I8W8F9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033234">
        <property type="term" value="P:negative regulation of protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043375">
        <property type="entry name" value="FSCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004011">
        <property type="entry name" value="Gyr_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004019">
        <property type="entry name" value="YLP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36135">
        <property type="entry name" value="FIBROUS SHEATH CABYR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36135:SF1">
        <property type="entry name" value="FIBROUS SHEATH CABYR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02756">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02757">
        <property type="entry name" value="YLP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00713">
        <property type="entry name" value="GYR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026024482" description="Fibrous sheath CABYR-binding protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="49077" checksum="3CD30133A087A085" modified="2020-08-12" version="1">MKFFVIAFAVIAAASAASIATDYLPPVDNNLEAASEQVELPAAEQGLLSDDGYRYKTVRRLKLRHRRDVNELPTAEYLPPVEEASEAVAVETPLADDGYRYKTVRRVIRRRRDVNELPTAEYLPPVEEASEAVAVETPLADDGYRYKTVRRVIRRRRDVNELPTAEYLPPVEEASESVAVETPLADDGYRYKTVRRVIRRRRDVNELPTAEYLPPVEEASEAVAVETPLADDGYRYKTVRRVIRRRRDVNELPTAEYLPPVEEASEAVAVETPLADDGYRYKTVRRVIRRRRDVNELSAEYLPPVEEASEAVAVETPLADDGYRYKTVRRVIRRRRDVNELPTAEYLPPVQEASEAVAVETPLADDGYRYKTVRRVIRRRRDVNELSAEYLPPVEEAASAVIDVPAEQTILANDGYRYKTVRRLKLRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W8N2</accession>
    <name>A0A6I8W8N2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117184811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239642.1">
        <property type="nucleotide sequence ID" value="XM_033383751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W8N2"/>
    <dbReference type="KEGG" id="dpo:117184811"/>
    <dbReference type="InParanoid" id="A0A6I8W8N2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033239642.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="26661" checksum="BD5EED47B0C55F30" modified="2020-08-12" version="1">MILHIMSRGTLRHFSMGPLRWSKSSSQEDAQTSDVLKTVAKKEPSALVVSVKAEAACEVTKDQPQDVKKSNTLYDAMKKMHNDYSKDYNEQKPGPTRISETISQTGTSQWRKSAPWPAYRGPFLEELLEKESPQVDVIPTNEKRKLTRRDYLRLIFKEERLKNPKEKKPVADTKMGQMEVDKEPLNPARLEFVNLKWKERQHEVGKRVQEADTKNNDSRFEMTTGLKGKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W937</accession>
    <name>A0A6I8W937_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Classical arabinogalactan protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6900783</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033239129.1">
        <property type="nucleotide sequence ID" value="XM_033383238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W937"/>
    <dbReference type="KEGG" id="dpo:6900783"/>
    <dbReference type="InParanoid" id="A0A6I8W937"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026344155" evidence="2">
        <location>
            <begin position="29"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033239129.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="19621" checksum="922A9204B4763002" modified="2020-08-12" version="1">MRPIRALFLAILAALMVLSFQLTPPATGSSSGTPTSTSPSGTPATGSSSGTPTSTSPGGTPTSSSPSGTPTSSSPSGTPASSSPSGTPASSSPSGTPASSSSSDTSPATTTTTTEATTTTEEATTKKHHRRIHRRRIVIIRRERGGGGNRRNRRNRRLRREGGNGGDVRRVRVHERRRVRLGRRFSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WA64</accession>
    <name>A0A6I8WA64_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Potassium voltage-gated channel protein Shaker-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6901446</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033240290.1">
        <property type="nucleotide sequence ID" value="XM_033384399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WA64"/>
    <dbReference type="KEGG" id="dpo:6901446"/>
    <dbReference type="InParanoid" id="A0A6I8WA64"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247938">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF113">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_033240290.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="20093" checksum="29F88418A3432CB9" modified="2020-08-12" version="1">MNIAIEKMKIECKSINAFRPVGFWGKIVGSLCVIAGVLTIALPVPVIVSNFNYFYHRETDQEEMQSQNFNHVTSCSYLPGALGQHLKKSSLSESSSDIMDLDDGIDATTPGLTDHTGRHMVPFLRTQQSFEKQQLQLQLQLQQQQAQQGQQQMGQNGLRSTNSLQLRHNNAMAVSIETDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WBJ7</accession>
    <name>A0A6I8WBJ7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC117185089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033240768.1">
        <property type="nucleotide sequence ID" value="XM_033384877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WBJ7"/>
    <dbReference type="KEGG" id="dpo:117185089"/>
    <dbReference type="InParanoid" id="A0A6I8WBJ7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="1">
        <location>
            <begin position="30"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033240768.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15703" checksum="A90E17E2C2FC68E7" modified="2020-08-12" version="1">MVALRSEIGSIKAKVCGEIRKPKVVLPCPPLTTIEELDHFEHSLQEESFFKNVIAELMMSGEKAFDKWIRSSWRSIVSDEVARQCSWRGTEEKKCIRGLRVTLAIRTGFKERFSLEDADFDRITQKFFQYAQDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WC86</accession>
    <accession>Q29F98</accession>
    <name>A0A6I8WC86_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">heat shock protein 27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC117184969</name>
        <name evidence="7" type="synonym">LOC4814158</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7 8">MV-25-SWS-2005</strain>
            <strain evidence="7">MV2-25</strain>
            <tissue evidence="7 8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354304.1">
        <property type="nucleotide sequence ID" value="XM_001354268.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033240174.1">
        <property type="nucleotide sequence ID" value="XM_033384283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WC86"/>
    <dbReference type="SMR" id="A0A6I8WC86"/>
    <dbReference type="GeneID" id="4814158"/>
    <dbReference type="KEGG" id="dpo:117184969"/>
    <dbReference type="KEGG" id="dpo:4814158"/>
    <dbReference type="OMA" id="GFPRTSM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078212">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WC86">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06526">
        <property type="entry name" value="metazoan_ACD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000042">
        <property type="entry name" value="heat shock protein 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF13">
        <property type="entry name" value="HEAT SHOCK PROTEIN 22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="80"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001354304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_033240174.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="25041" checksum="5111D8A88EE4C8E7" modified="2020-08-12" version="1">MSLVPLLSLARELDHDYRSAYNEWDHFLEDDFGFGVHAHDLFQRPRLMLPHHGSTLGRRRFLPYERSHHHGHPHQLVTRRQSGGGQNSLIPAIGKDGFQVCMDVSQFKPNELTVKVVDKTVVVEGKHEEREDGHGMIQRHFVRKYTLPKDFDPNEVVSTVSSDGVLTLKAPPPPSKEQPKQERIVQIQQTGPAHLSVKAPEGAAAAAAADGKTENGGGEKMETGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCD6</accession>
    <name>A0A6I8WCD6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein ROP isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Rop</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240224.1">
        <property type="nucleotide sequence ID" value="XM_033384333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCD6"/>
    <dbReference type="FunCoup" id="A0A6I8WCD6">
        <property type="interactions" value="1602"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8WCD6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8WCD6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051650">
        <property type="term" value="P:establishment of vesicle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.60:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_033240224.1"/>
        </source>
    </evidence>
    <sequence length="620" mass="71005" checksum="351355A4417C37B1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCG0</accession>
    <name>A0A6I8WCG0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein TwdlY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TwdlY</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241078.1">
        <property type="nucleotide sequence ID" value="XM_033385187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WCG0"/>
    <dbReference type="FunCoup" id="A0A6I8WCG0">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815812"/>
    <dbReference type="InParanoid" id="A0A6I8WCG0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062129">
        <property type="term" value="C:chitin-based extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008010">
        <property type="term" value="F:structural constituent of chitin-based larval cuticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040003">
        <property type="term" value="P:chitin-based cuticle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004145">
        <property type="entry name" value="DUF243"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927:SF2">
        <property type="entry name" value="FI07246P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31927">
        <property type="entry name" value="FI07246P-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03103">
        <property type="entry name" value="DUF243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00690">
        <property type="entry name" value="DM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026095389" evidence="2">
        <location>
            <begin position="20"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="DUF243" evidence="3">
        <location>
            <begin position="78"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033241078.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="27089" checksum="E5AC176E221E068B" modified="2020-08-12" version="1">MSVRPILISCLALLAVAQARPQYGYEQPGSGPSDNFIGGSGGGGAHVIGGGIGGGLVAIQPHRGGDKYLPPASTTQAPIINKKFYLVSAPEDHSNDGKVKHLVLGRPQKNYRVVFIKAPAGDNANVKYSAEFAPQEEKTVIYVLSKKDNDLDATDIATPAPTQPSKPEVFFIKYKTDDEAKQAQHEIQGQYDKLGGTNEYQEDNNAPITSVIGSLDGLNPDGSYNYRQIGNRPSSGPNSSLSPSAQYLPSLLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WDM9</accession>
    <name>A0A6I8WDM9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein C1683.06c-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6899165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033241525.1">
        <property type="nucleotide sequence ID" value="XM_033385634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8WDM9"/>
    <dbReference type="FunCoup" id="A0A6I8WDM9">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899165"/>
    <dbReference type="InParanoid" id="A0A6I8WDM9"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033241525.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="32844" checksum="F4EB521FA95CA670" modified="2020-08-12" version="1">MRRIPNASDRLDVPIYLGAVEPLISCSEDDKKYFHGCDGFGDCLAACDSEESLESIVQAEHAVTAIYKLCRQRPKQITVFAVWPLTNLSLGFTMYGEEFGQQLRDLYIMGGNYQGVGNSSRSAEFNFHSDPEAAHAVLLKTHCPITILPWEACLPERFNIHINWRLEQFAVRAAAVQHSAITLLNRVESAQWLPMMEQYGIDTWNPCDAIVVAVWLFGDRLVRKQSTWHATVDLRGTHTRGQMVLDHLREREKYPENVRIIELVDAEFFKRIVEWVAGLDGGEFLFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-06-10" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>B2L9T6</accession>
    <accession>D2SXZ0</accession>
    <name>B2L9T6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase complex subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 18" type="primary">ATP8</name>
        <name evidence="16" type="synonym">atp8</name>
        <name evidence="18" type="ORF">HCS66_mgp13</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="15 18"/>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2008" name="Biol. Lett." volume="4" first="195" last="199">
            <title>Out of Hawaii: the origin and biogeography of the genus Scaptomyza (Diptera: Drosophilidae).</title>
            <authorList>
                <person name="O'Grady P.M."/>
                <person name="DeSalle R."/>
            </authorList>
            <dbReference type="PubMed" id="18296276"/>
            <dbReference type="DOI" id="10.1098/rsbl.2007.0575"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2008-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="O'Grady P.M."/>
                <person name="DeSalle R."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="journal article" date="2009" name="Nucleic Acids Res." volume="37" first="7509" last="7518">
            <title>Expression profiling of Drosophila mitochondrial genes via deep mRNA sequencing.</title>
            <authorList>
                <person name="Torres T.T."/>
                <person name="Dolezal M."/>
                <person name="Schlotterer C."/>
                <person name="Ottenwalder B."/>
            </authorList>
            <dbReference type="PubMed" id="19843606"/>
            <dbReference type="DOI" id="10.1093/nar/gkp856"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2009-04" db="EMBL/GenBank/DDBJ databases">
            <title>Characterization of Drosophila mitochondrial transcripts through high throughput EST data.</title>
            <authorList>
                <person name="Torres T.T."/>
                <person name="Dolezal M."/>
                <person name="Schlotterer C."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17 18" key="5">
        <citation type="submission" date="2019-12" db="EMBL/GenBank/DDBJ databases">
            <title>Evolutionary conservation of topologically associating domains and their influences on genome synteny and structural variations in Drosophila.</title>
            <authorList>
                <person name="Liao Y."/>
                <person name="Chakraborty M."/>
                <person name="Zhang X."/>
                <person name="Emerson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">MV2-25</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="17 18" key="6">
        <citation type="submission" date="2020-03" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="NCBI Genome Project"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">MV2-25</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <reference evidence="18" key="7">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion membrane</location>
            <topology evidence="1 13">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ATPase protein 8 family.</text>
    </comment>
    <dbReference type="EMBL" id="EU493762">
        <property type="protein sequence ID" value="ACC94847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="FJ899745">
        <property type="protein sequence ID" value="ACP04300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_009742596.1">
        <property type="nucleotide sequence ID" value="NC_046603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B2L9T6"/>
    <dbReference type="STRING" id="46245.B2L9T6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Mitochondrion MT"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001421">
        <property type="entry name" value="ATP8_metazoa"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00895">
        <property type="entry name" value="ATP-synt_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0138">CF(0)</keyword>
    <keyword evidence="7 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ACC94847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ACP04300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="YP_009742596.1"/>
        </source>
    </evidence>
    <sequence length="53" mass="6308" checksum="C841C69F7E07F9F7" modified="2008-06-10" version="1">MPQMAPISWLLLFIVFSITFILFCSINYYSFMPSSPKSNELKNINLNSMNWKW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>B5DI68</accession>
    <name>U518_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>FHIP family protein GA25918</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA25918</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the FHIP family.</text>
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    <dbReference type="EMBL" id="CH379060">
        <property type="protein sequence ID" value="EDY70008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DI68"/>
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        <property type="interactions" value="70"/>
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    <dbReference type="STRING" id="46245.B5DI68"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0282010">
        <property type="protein sequence ID" value="FBpp0280448"/>
        <property type="gene ID" value="FBgn0247295"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3695">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007807_0_0_1"/>
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    <dbReference type="OMA" id="RMPSLVQ"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247295">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019384">
        <property type="entry name" value="FHIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045669">
        <property type="entry name" value="FHIP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045668">
        <property type="entry name" value="FHIP_KELAA_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705:SF11">
        <property type="entry name" value="FHIP FAMILY PROTEIN CG3558"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR21705">
        <property type="entry name" value="RAI16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19314">
        <property type="entry name" value="DUF5917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19311">
        <property type="entry name" value="KELAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10257">
        <property type="entry name" value="RAI16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000379012" description="FHIP family protein GA25918">
        <location>
            <begin position="1"/>
            <end position="1081"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="650"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="830"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="933"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="657"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="840"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="857"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="891"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="933"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="954"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="964"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="994"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1081" mass="118337" checksum="3EF804D8855AD7CB" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>B5DJZ3</accession>
    <accession>A0A6I8UY67</accession>
    <name>B5DJZ3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein NimB3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NimB3</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002133215.1">
        <property type="nucleotide sequence ID" value="XM_002133179.3"/>
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    <dbReference type="AlphaFoldDB" id="B5DJZ3"/>
    <dbReference type="FunCoup" id="B5DJZ3">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="GeneID" id="6902701"/>
    <dbReference type="KEGG" id="dpo:6902701"/>
    <dbReference type="eggNOG" id="KOG1218">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2029146_0_0_1"/>
    <dbReference type="InParanoid" id="B5DJZ3"/>
    <dbReference type="OMA" id="GNCECGP"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0250415">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DJZ3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002133215.1"/>
        </source>
    </evidence>
    <sequence length="120" mass="13728" checksum="7644EDF96FC4B5E8" modified="2008-10-14" version="1">MHLKSPLIGLLIVCLAIERPSPTAAQFWLKLPEQNWEREMLATRDSGVCYKEEMVETINPNARLRQISYCCEGYVNHGSPQNLKCQPICTEDCAHGLCLSPGYCECAPGYYREERRCKQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>B5DK76</accession>
    <accession>A0A6I8UYG9</accession>
    <name>B5DK76_DROPS</name>
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        <submittedName>
            <fullName evidence="5">Cohesin subunit SA-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SA1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCC3 family.</text>
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    <dbReference type="RefSeq" id="XP_002133300.1">
        <property type="nucleotide sequence ID" value="XM_002133264.3"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_005067_0_0_1"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003682">
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056396">
        <property type="entry name" value="HEAT_SCC3-SA"/>
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    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24571">
        <property type="entry name" value="HEAT_SCC3-SA"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1089"/>
            <end position="1128"/>
        </location>
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        <location>
            <begin position="290"/>
            <end position="317"/>
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    </feature>
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        <location>
            <begin position="10"/>
            <end position="26"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1109"/>
            <end position="1122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002133300.1"/>
        </source>
    </evidence>
    <sequence length="1128" mass="130292" checksum="8706C1CAD54D64AD" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>B5DN16</accession>
    <accession>A0A6I8UZK7</accession>
    <name>B5DN16_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sperm flagellar protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6901510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134196.1">
        <property type="nucleotide sequence ID" value="XM_002134160.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DN16"/>
    <dbReference type="SMR" id="B5DN16"/>
    <dbReference type="STRING" id="46245.B5DN16"/>
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    <dbReference type="KEGG" id="dpo:6901510"/>
    <dbReference type="HOGENOM" id="CLU_137557_1_0_1"/>
    <dbReference type="InParanoid" id="B5DN16"/>
    <dbReference type="OMA" id="QVYTPRC"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247647">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DN16">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010441">
        <property type="entry name" value="CH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06294">
        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002134196.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="15356" checksum="F296B676FD4C9204" modified="2008-10-14" version="1">MSGFRVLDEEDRQMLISWLKEHNIELNHRTRTELRDAFAVAKICKRIHPDLVDLQVYTPRCSMTQMLNNWKIFNDRVLKKLDLNLSNLMLKELAGGAACAIESILYKLMTTEYALKRGEGKLTLAKKPLDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>B5DNJ2</accession>
    <accession>A0A6I8UZV7</accession>
    <name>B5DNJ2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC6901707</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134375.1">
        <property type="nucleotide sequence ID" value="XM_002134339.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DNJ2"/>
    <dbReference type="GeneID" id="6901707"/>
    <dbReference type="KEGG" id="dpo:6901707"/>
    <dbReference type="eggNOG" id="KOG1744">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075666_2_0_1"/>
    <dbReference type="OMA" id="ELAKHAX"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0244925">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000016">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="13 14" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="16">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002134375.1"/>
        </source>
    </evidence>
    <sequence length="127" mass="14106" checksum="97E831EA9C537CB1" modified="2008-10-14" version="1">MPPKTASGNATKKAGKAQKNIHINTTDKKKRRKVRKESYAIYIYKVLKQVHPDTGVSSKAMSIMNSFVNDIFERIASEASRLGHYNKKSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>B5DR36</accession>
    <accession>A0A6I8V123</accession>
    <name>B5DR36_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein LLP homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6900042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the learning-associated protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002135406.1">
        <property type="nucleotide sequence ID" value="XM_002135370.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DR36"/>
    <dbReference type="FunCoup" id="B5DR36">
        <property type="interactions" value="1186"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DR36"/>
    <dbReference type="GeneID" id="6900042"/>
    <dbReference type="KEGG" id="dpo:6900042"/>
    <dbReference type="eggNOG" id="KOG4811">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148891_0_0_1"/>
    <dbReference type="InParanoid" id="B5DR36"/>
    <dbReference type="OMA" id="YGNYPVW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0249710">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DR36">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001099">
        <property type="term" value="F:basal RNA polymerase II transcription machinery binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097484">
        <property type="term" value="P:dendrite extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018784">
        <property type="entry name" value="LLPH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34253">
        <property type="entry name" value="PROTEIN LLP HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34253:SF1">
        <property type="entry name" value="PROTEIN LLP HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10169">
        <property type="entry name" value="LLPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002135406.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="17273" checksum="55E97742FDD04DA7" modified="2008-10-14" version="1">MGRQNRSRKRRDEMNKIKKARYDAKELIRLKKTLGLLDADGNEIMKDIADVVEIKTSKEIKRDAKTREQQELIYEHQESLEKGQKVKVTNEKTGVEHVFNSKTLKDQYGNYPAWFKKKKTAKRLRKKQHSQTKNFKQAWTTVNVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DA6</accession>
    <accession>A0A6I8UBV3</accession>
    <name>Q29DA6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil-helix-coiled-coil-helix domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC4812313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CHCHD7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353007.1">
        <property type="nucleotide sequence ID" value="XM_001352971.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DA6"/>
    <dbReference type="FunCoup" id="Q29DA6">
        <property type="interactions" value="552"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DA6"/>
    <dbReference type="GeneID" id="4812313"/>
    <dbReference type="KEGG" id="dpo:4812313"/>
    <dbReference type="eggNOG" id="KOG4618">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175044_0_0_1"/>
    <dbReference type="InParanoid" id="Q29DA6"/>
    <dbReference type="OMA" id="QELSYKC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078023">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033108">
        <property type="term" value="P:mitochondrial respiratory chain complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051040">
        <property type="entry name" value="COX23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353007.1"/>
        </source>
    </evidence>
    <sequence length="85" mass="10042" checksum="0CD8B9736DC39BFE" modified="2006-04-04" version="1">MPRNLNAERDNPCLKEQELSFKCLNKNNFDRDKCEVYFANYNNCKEFWNKVRADRRGKGIAPYLPPLEERDAIKAEYLKAKPSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DK1</accession>
    <accession>A0A6I8UBM8</accession>
    <name>Q29DK1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC4812079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001352912.1">
        <property type="nucleotide sequence ID" value="XM_001352876.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DK1"/>
    <dbReference type="FunCoup" id="Q29DK1">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29DK1"/>
    <dbReference type="GeneID" id="4812079"/>
    <dbReference type="KEGG" id="dpo:4812079"/>
    <dbReference type="eggNOG" id="ENOG502S2JM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048495_1_0_1"/>
    <dbReference type="InParanoid" id="Q29DK1"/>
    <dbReference type="OMA" id="ICNDVSD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079892">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DK1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00091">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.570.10:FF:000007">
        <property type="entry name" value="Alkaline nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF19">
        <property type="entry name" value="NUCLEASE EXOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="6">
        <location>
            <begin position="169"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_001352912.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="47971" checksum="B5755F10924739EF" modified="2006-04-04" version="1">MKSVRLTLVLIGLVVVQAAWARVPYPEVELPPIVQEDEIYERAVYVAPQPEGRAAACSVAIRGALPSPQPVYLKTDSEDFYPFNDVGVMEFNAGDSFQLWCPGAFNTHSETLLTAKCVSGSTFSVDGKDFEFKDLYCKAWPGFKAIKSGATCNGGVVIRVGFEITSTRFAEQMQICFNEEEEVTRYTRHKLEPGSNYYETGVARITFQTAGYFDGKNVDKLYTQVTQMETINKDLGGDADQYFDTATNVFLARGHLGAKADFDYAPEQRATFLFINAAPQWQTFNAGNWARVEDGLRAWVSKNKMNVNCYTGVWGVTTLPNKDGVETPLYLARDANNNGLIPVPKLYFRVVIEPSTHRGIVFVGVNNPHLSEEQIKRDYVLCDDVSDQVTYINWKKDDIKTGWSYACEVADFLKTVKHLPALTAKGGLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GS6</accession>
    <accession>A0A6I8UG77</accession>
    <name>Q29GS6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Palmitoyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ppt1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19234"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the palmitoyl-protein thioesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="RefSeq" id="XP_001354977.1">
        <property type="nucleotide sequence ID" value="XM_001354941.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GS6"/>
    <dbReference type="SMR" id="Q29GS6"/>
    <dbReference type="FunCoup" id="Q29GS6">
        <property type="interactions" value="1697"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29GS6"/>
    <dbReference type="ESTHER" id="drops-q29gs6">
        <property type="family name" value="Palmitoyl-protein_thioesterase"/>
    </dbReference>
    <dbReference type="GlyCosmos" id="Q29GS6">
        <property type="glycosylation" value="1 site, No reported glycans"/>
    </dbReference>
    <dbReference type="GeneID" id="4815256"/>
    <dbReference type="KEGG" id="dpo:4815256"/>
    <dbReference type="eggNOG" id="KOG2541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050129_0_0_1"/>
    <dbReference type="InParanoid" id="Q29GS6"/>
    <dbReference type="OMA" id="KFVMVMF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071455">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29GS6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000107">
        <property type="entry name" value="Palmitoyl-protein thioesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002472">
        <property type="entry name" value="Palm_thioest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF8">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02089">
        <property type="entry name" value="Palm_thioest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00414">
        <property type="entry name" value="PPTHIESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001354977.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="35947" checksum="D9F73A33AB8841E5" modified="2006-04-04" version="1">MVRPRIINFNIIQLNIGLKKAAVFSVFLAAVYFVVGFGTKTGSTLPVVLWHGMGDTCCLPFSLGAIKTLIEEHVNGTYVRSLKIGGNVVLDYESGFFIHPNEQVEYVCKHLAQDERLAEGYNAIGFSQGGQFLRAVAQRCPEPPMRTLITLGGQHQGVFGLPMCPTLTATSCEYISRLLNVAAYEEWVQAELVQATYWHDPLHENQYRLGSTFLADINNELYLNENYVENLNKLKKFVMVMFLNDTIVQPKESQWFQFYTSGQDRVIQPFNESKVYQDLGLDRMLREGKLQFLGIEGDHLAISKAWFIEHLVPLLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IB2</accession>
    <accession>A0A6I8UHN9</accession>
    <name>Q29IB2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin-related protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Arp6</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin family. ARP6 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001355682.1">
        <property type="nucleotide sequence ID" value="XM_001355646.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29IB2"/>
    <dbReference type="SMR" id="Q29IB2"/>
    <dbReference type="FunCoup" id="Q29IB2">
        <property type="interactions" value="583"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29IB2"/>
    <dbReference type="GeneID" id="4816025"/>
    <dbReference type="KEGG" id="dpo:4816025"/>
    <dbReference type="eggNOG" id="KOG0680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_1_1_1"/>
    <dbReference type="InParanoid" id="Q29IB2"/>
    <dbReference type="OMA" id="FFEEYEC"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071193">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd10210">
        <property type="entry name" value="ASKHA_NBD_Arp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000003">
        <property type="entry name" value="Actin-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000014">
        <property type="entry name" value="Putative actin-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001355682.1"/>
        </source>
    </evidence>
    <sequence length="398" mass="45481" checksum="03BF7AB4750B49EF" modified="2006-04-04" version="1">MASTVVVLDNGAHTAKVGLANQEEPRVVPNCIMKAKSERRRAFVGNQIEDCRDTSALFYILAFQRGYLLNWDTQKTVWDYIFSKEGIGCSLDNRNIVITEPQMNFPSVQEAMLEMLFEQYRVAGMYKTTAADLAAFNYVADSEERTTMQTLNCIIIDVGYSFTHIVPFVLGRRVLEGIRRIDMGGKALTNHLKELISYRQLNMMDESHVVNQIKEDVCFVAEDFKEAMRVHQCEKKRKEVAIDYVLPDFTTIKRGYVRIPGRPRMDEETMQTVPLCNERFTVPELLFNPSDIGIHQVGIPEAVADCLKACPWQAHRELLLNILIVGGCAQFPGFLPRLKKDLRALVPDDLEVSLICPEDPVRYAWYGGREVATSPNFEEFVYTRDDYEEFGVHGLNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JA0</accession>
    <accession>A0A6I8UGZ0</accession>
    <name>Q29JA0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein orion isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">orion</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001355344.3">
        <property type="nucleotide sequence ID" value="XM_001355308.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JA0"/>
    <dbReference type="FunCoup" id="Q29JA0">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JA0"/>
    <dbReference type="GeneID" id="4816053"/>
    <dbReference type="KEGG" id="dpo:4816053"/>
    <dbReference type="HOGENOM" id="CLU_015334_0_0_1"/>
    <dbReference type="InParanoid" id="Q29JA0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0068743">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032062">
        <property type="entry name" value="DUF4803"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47890">
        <property type="entry name" value="LD24308P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47890:SF1">
        <property type="entry name" value="LD24308P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16061">
        <property type="entry name" value="DUF4803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001355344.3"/>
        </source>
    </evidence>
    <sequence length="669" mass="76983" checksum="07517FB5AAEC4C6D" modified="2016-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29JG4</accession>
    <accession>A0A6I8UGU9</accession>
    <name>Q29JG4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein mh</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mh</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355280.1">
        <property type="nucleotide sequence ID" value="XM_001355244.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29JG4"/>
    <dbReference type="SMR" id="Q29JG4"/>
    <dbReference type="FunCoup" id="Q29JG4">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29JG4"/>
    <dbReference type="GeneID" id="4816020"/>
    <dbReference type="KEGG" id="dpo:4816020"/>
    <dbReference type="eggNOG" id="KOG3931">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019426_3_0_1"/>
    <dbReference type="InParanoid" id="Q29JG4"/>
    <dbReference type="OMA" id="AYKTHIW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081594">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29JG4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044245">
        <property type="entry name" value="Spartan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006640">
        <property type="entry name" value="SprT-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055220">
        <property type="entry name" value="SPRTN_ZBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21220:SF0">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE SPRTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10263">
        <property type="entry name" value="SprT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22934">
        <property type="entry name" value="SPRTN_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00731">
        <property type="entry name" value="SprT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="352"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="638"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="352"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="398"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="644"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="693"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001355280.1"/>
        </source>
    </evidence>
    <sequence length="752" mass="84593" checksum="1D15849ECFCECEB2" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KI4</accession>
    <accession>A0A6I8UIP6</accession>
    <name>Q29KI4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein Jhbp14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Jhbp14</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356129.2">
        <property type="nucleotide sequence ID" value="XM_001356093.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KI4"/>
    <dbReference type="FunCoup" id="Q29KI4">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KI4"/>
    <dbReference type="GeneID" id="4816513"/>
    <dbReference type="KEGG" id="dpo:4816513"/>
    <dbReference type="eggNOG" id="ENOG502S5NE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069908_2_0_1"/>
    <dbReference type="InParanoid" id="Q29KI4"/>
    <dbReference type="OMA" id="DMRIYAN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0079187">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KI4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000002">
        <property type="entry name" value="GL26314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF18">
        <property type="entry name" value="BCDNA.GH05536-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001356129.2"/>
        </source>
    </evidence>
    <sequence length="263" mass="29774" checksum="40667BAAEE4DDB81" modified="2008-10-14" version="2">MKLLWNIVLVALCGVSLAEDFEPELPPVEFKPVRELSGDIPTCRESDLNINECIKQGLQQITPRMKYGISELNIPPLDPFEMGKSSYSYNSGLLQGRIAMKNVVVHGLSEGIVDKVNFRLKNGQVRMEILSHVPQMLVEGAYKADIKLNDLKLTPKGTFNVTLTDVTMRARPTGELYERDGHTYLRLTKLETEPKVGDLRFYANGLVPDPALNDVILDFINQYWRQLYQAMLPETLATWQPLILKSSNDFFAALPFDMLVTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KS9</accession>
    <accession>A0A6I8V9K0</accession>
    <name>Q29KS9_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arp2/3 complex 34 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">Arpc2</name>
        <name evidence="10" type="synonym">LOC4816687</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11 12">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="10 11 12">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ARPC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356036.1">
        <property type="nucleotide sequence ID" value="XM_001356000.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036659.1">
        <property type="nucleotide sequence ID" value="XM_015181173.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015036660.1">
        <property type="nucleotide sequence ID" value="XM_015181174.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KS9"/>
    <dbReference type="SMR" id="Q29KS9"/>
    <dbReference type="STRING" id="46245.Q29KS9"/>
    <dbReference type="GeneID" id="4816687"/>
    <dbReference type="KEGG" id="dpo:4816687"/>
    <dbReference type="eggNOG" id="KOG2826">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059439_2_0_1"/>
    <dbReference type="OMA" id="FRSYFHY"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070720">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29KS9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000002">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000004">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007188">
        <property type="entry name" value="ARPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058">
        <property type="entry name" value="ARP2/3 COMPLEX 34 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04045">
        <property type="entry name" value="P34-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001356036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_015036659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_015036660.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="35276" checksum="3543E3F254A8B4AC" modified="2006-04-04" version="1">MILLEINNRIIEETLLVKYRNAQAGLKPESIDIRIADFDGVLYHISNVNNDKTKVRISISLKFYKQLQEHGADELLKREYGSLLTDTEEGYNVSVLINLEEIPEDWEQIAKRIGLLKRNCFASVFEKYFDYQEQGEEGQKRAVINYRNDETLYVEAKPDRVTVVFSTIFRDEDDVVIGKVFMQELREGRRASHTAPQVLFTHREPPLELANTDARVGDNIGYVTFVLFPRHTNKETRDNTINLIHMFRDYLHYHIKCSKAYIHSRMRAKTSDFLKVLNRARPEPKNTEKKTITGRTFKRME</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q29L06</accession>
    <accession>A0A6I8UIC6</accession>
    <name>Q29L06_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Reticulocalbin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4816682</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable molecular chaperone assisting protein biosynthesis and transport in the endoplasmic reticulum. Required for the proper biosynthesis and transport of pulmonary surfactant-associated protein A/SP-A, pulmonary surfactant-associated protein D/SP-D and the lipid transporter ABCA3. By regulating both the proper expression and the degradation through the endoplasmic reticulum-associated protein degradation pathway of these proteins plays a crucial role in pulmonary surfactant homeostasis. Has an anti-fibrotic activity by negatively regulating the secretion of type I and type III collagens. This calcium-binding protein also transiently associates with immature PCSK6 and regulates its secretion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PCSK6 (immature form including the propeptide); probably involved in the maturation and the secretion of PCSK6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001355959.1">
        <property type="nucleotide sequence ID" value="XM_001355923.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29L06"/>
    <dbReference type="FunCoup" id="Q29L06">
        <property type="interactions" value="2042"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29L06"/>
    <dbReference type="GeneID" id="4816682"/>
    <dbReference type="KEGG" id="dpo:4816682"/>
    <dbReference type="eggNOG" id="KOG4223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044718_0_0_1"/>
    <dbReference type="InParanoid" id="Q29L06"/>
    <dbReference type="OMA" id="HELTQWN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076382">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29L06">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16227">
        <property type="entry name" value="EFh_CREC_RCN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000104">
        <property type="entry name" value="calumenin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000424">
        <property type="entry name" value="GM18042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF95">
        <property type="entry name" value="LD34388P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="93"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="181"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001355959.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="40052" checksum="73A1CA7311AA6B88" modified="2006-04-04" version="1">MPRNNMRTFPLVLCTVAVFAAVGPMPAFGAVATHKHSAHENHLSKERVKDGIYAPRDAHHHGEDGEHNVEFDHEAIIGNTKEAQEFDTLSPEESKRRLLVLVKLMDLNKDEFVDRHELKAWILRSFKKLSEEEAADRFDEIDQETDERITWKEYLQDTYSMEDENFKKETIDFDNYEEEQKMIKQDKEMFNAADINKDGVLSLEEFVYFHNPEEHPQMLPILLEHTMQDKDLNHDGKINFQEFVGEAASHHDKEWLLTEKERFDKDHDINGDGVLTGNEVLSWIVPSNTAIASDEVDHLFVSTDEDHDDRLSYLEILNNYETFVGSEATDYGDHLQNINHLADEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q29N70</accession>
    <name>COG3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Conserved oligomeric Golgi complex subunit 3</fullName>
            <shortName>COG complex subunit 3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Component of oligomeric Golgi complex 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Cog3</name>
        <name type="ORF">GA16936</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in ER-Golgi transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the conserved oligomeric Golgi complex which is composed of eight different subunits and is required for normal Golgi morphology and localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the COG3 family.</text>
    </comment>
    <comment type="sequence caution" evidence="7">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL-CDS" id="EAL33473" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CH379060">
        <property type="protein sequence ID" value="EAL33473.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29N70"/>
    <dbReference type="SMR" id="Q29N70"/>
    <dbReference type="FunCoup" id="Q29N70">
        <property type="interactions" value="1469"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29N70"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0281778">
        <property type="protein sequence ID" value="FBpp0280216"/>
        <property type="gene ID" value="FBgn0076950"/>
    </dbReference>
    <dbReference type="GeneID" id="4817142"/>
    <dbReference type="KEGG" id="dpo:4817142"/>
    <dbReference type="CTD" id="83548"/>
    <dbReference type="eggNOG" id="KOG2604">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011639_1_1_1"/>
    <dbReference type="InParanoid" id="Q29N70"/>
    <dbReference type="OMA" id="LEEHMQY"/>
    <dbReference type="PhylomeDB" id="Q29N70"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076950">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048685">
        <property type="entry name" value="COG3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048320">
        <property type="entry name" value="COG3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20671">
        <property type="entry name" value="COG3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04136">
        <property type="entry name" value="COG3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0333">Golgi apparatus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000306271" description="Conserved oligomeric Golgi complex subunit 3">
        <location>
            <begin position="1"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="874"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="874"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="884"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q14746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q961G1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="4">
        <source>
            <dbReference type="UniProtKB" id="Q96JB2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5"/>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="EMBL" id="EAL33473.1"/>
        </source>
    </evidence>
    <sequence length="914" mass="101832" checksum="DB0FEC0F5B834F75" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LYN9</accession>
    <accession>A0A6I8UE93</accession>
    <name>Q2LYN9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4813921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354084.2">
        <property type="nucleotide sequence ID" value="XM_001354048.4"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LYN9"/>
    <dbReference type="GeneID" id="4813921"/>
    <dbReference type="KEGG" id="dpo:4813921"/>
    <dbReference type="eggNOG" id="ENOG502QUZ3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002769_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LYN9"/>
    <dbReference type="OMA" id="MRYIDEE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076915">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.120">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40552">
        <property type="entry name" value="AT05186P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40552:SF6">
        <property type="entry name" value="FI09606P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1357"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1077"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1361"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1374"/>
            <end position="1383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354084.2"/>
        </source>
    </evidence>
    <sequence length="1732" mass="199707" checksum="A2E9C39A7899F646" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ67</accession>
    <accession>A0A6I8UDX9</accession>
    <name>Q2LZ67_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">CSN12-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PCID2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353906.2">
        <property type="nucleotide sequence ID" value="XM_001353870.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ67"/>
    <dbReference type="SMR" id="Q2LZ67"/>
    <dbReference type="FunCoup" id="Q2LZ67">
        <property type="interactions" value="2117"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZ67"/>
    <dbReference type="GeneID" id="4813813"/>
    <dbReference type="KEGG" id="dpo:4813813"/>
    <dbReference type="eggNOG" id="KOG2688">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031567_2_0_1"/>
    <dbReference type="InParanoid" id="Q2LZ67"/>
    <dbReference type="OMA" id="INRMFTL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080281">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000146">
        <property type="entry name" value="PCI domain-containing protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001353906.2"/>
        </source>
    </evidence>
    <sequence length="396" mass="45450" checksum="573FF4891BEA1CB7" modified="2008-10-14" version="2">MFGSLANYLSNVERCAEACSGQALSDFLSLRDVHVQNDKLHVAQPEKMVERYLDAPLDEVVGAHLKVIYYLAQNPTNYMEAYTQQAACCSAVVKLLQMMKDENWCLPVMYRVCLDLRYLAQACEKHGRSFTPGQILEKAADCMMACFRVCAADGRASEEETKRLGMMNLVNQLFKIYFRINKLHLCKPLIRAIDNCAFKDTFPLPEQITYKYFVGRRAMFDSNYRMAVEDLSYAFTHCPDRFGSNKRLILIYLVPVKMLLGYLPSKVLLQRHDLLLFHDLAQTLKAGNVNKFDEIVRENEKVLIRSGIYLLVEKLKFIVYRNLFKKVFAIRQTHQLDLGDFMTALQFVGVTDVSLDETHCIIANLIYEGKIKGYISYAHNKLVVSKQNPFPPLISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZ91</accession>
    <accession>A0A6I8UDQ1</accession>
    <name>Q2LZ91_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase subunit b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATPsynB</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic ATPase B chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353882.1">
        <property type="nucleotide sequence ID" value="XM_001353846.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZ91"/>
    <dbReference type="SMR" id="Q2LZ91"/>
    <dbReference type="FunCoup" id="Q2LZ91">
        <property type="interactions" value="1220"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZ91"/>
    <dbReference type="GeneID" id="4813471"/>
    <dbReference type="KEGG" id="dpo:4813471"/>
    <dbReference type="eggNOG" id="KOG3976">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087186_1_0_1"/>
    <dbReference type="InParanoid" id="Q2LZ91"/>
    <dbReference type="OMA" id="PEEWFTF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080871">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2210:FF:000003">
        <property type="entry name" value="ATP synthase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008688">
        <property type="entry name" value="ATP_synth_Bsub_B/MI25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05405">
        <property type="entry name" value="Mt_ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161060">
        <property type="entry name" value="ATP synthase B chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0138">CF(0)</keyword>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001353882.1"/>
        </source>
    </evidence>
    <sequence length="245" mass="27534" checksum="5071094F2E41C54F" modified="2006-02-21" version="1">MFSRAALLTAQRPLAVAATRSAAAAAGQSGSGSIERRQRPEHPGKVRLGFVPEEWFQFFYNKTGVTGPYTFGVGLITYLCSKEIYVMEHEYYSGLSLGIMAVIAVKKLGPVIAKWADGEIDKIESEWKEGREAELKVLSDAIEAEKKEQWRADGALLLMDAKKENIALQLEAAFRERAMNVYSEVKRRLDYQVECRHVERRLNQKHMVNWITTNVLASISPQQEKETLNKCIADLSALALRVKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZA8</accession>
    <accession>A0A6I8UDW5</accession>
    <name>Q2LZA8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Proliferation-associated protein 2G4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4813221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353865.1">
        <property type="nucleotide sequence ID" value="XM_001353829.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZA8"/>
    <dbReference type="SMR" id="Q2LZA8"/>
    <dbReference type="FunCoup" id="Q2LZA8">
        <property type="interactions" value="2553"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZA8"/>
    <dbReference type="GeneID" id="4813221"/>
    <dbReference type="KEGG" id="dpo:4813221"/>
    <dbReference type="eggNOG" id="KOG2776">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041451_2_0_1"/>
    <dbReference type="InParanoid" id="Q2LZA8"/>
    <dbReference type="OMA" id="SRMFYSE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070464">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZA8">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="CDD" id="cd01089">
        <property type="entry name" value="PA2G4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000013">
        <property type="entry name" value="DNA-binding protein, 42 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000029">
        <property type="entry name" value="Proliferation-associated 2G4, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004545">
        <property type="entry name" value="PA2G4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00495">
        <property type="entry name" value="crvDNA_42K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF11">
        <property type="entry name" value="PROLIFERATION-ASSOCIATED PROTEIN 2G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="2">
        <location>
            <begin position="20"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353865.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="42613" checksum="4C00E41AAC08646A" modified="2006-02-21" version="1">MADAEKEPEKTIAEDLVVTKYKLAGEIVNKTLKAVIGLCVADASVREICTQGDNLLTEETGKVYKKEKDLKKGIAFPTCLSVNNCVCHFSPAKNDTDYTLKAGDVVKIDLGAHIDGFIAVAAHTISVGASADQKISGRPADVILAAYWSVQAALRLLKSGANNYAITDAVQQISESYKCKPIEGMLSHELKQFKIDGEKTIIQNPSEAQRKEHEKCTFDTYEVYAIDVIVSSGEGVGREKDTKVSIYKKSEENYMLKLKASRALLAEVKTKYGNMPFNIRSFEEETKARMGVVECVSHKMIEPFQVLYEKPTEIVAQFKHTILLMPNGVNLVTGIPFNVDSFASEYSIAQEELKTLVAQPLGPVKGKGKGKKASAAATKVDSAPAPVEAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZT4</accession>
    <accession>A0A6I8UDF5</accession>
    <name>Q2LZT4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein stwl isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">stwl</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001353689.1">
        <property type="nucleotide sequence ID" value="XM_001353653.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZT4"/>
    <dbReference type="FunCoup" id="Q2LZT4">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="GeneID" id="4813024"/>
    <dbReference type="KEGG" id="dpo:4813024"/>
    <dbReference type="eggNOG" id="ENOG502QR16">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_293256_0_0_1"/>
    <dbReference type="InParanoid" id="Q2LZT4"/>
    <dbReference type="OMA" id="PSENSWD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077730">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZT4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004210">
        <property type="entry name" value="BESS_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF60">
        <property type="entry name" value="SI:CH211-15D5.12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02944">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51031">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="707"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="863"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="982"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="864"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="906"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="992"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001353689.1"/>
        </source>
    </evidence>
    <sequence length="1002" mass="107610" checksum="066F99554E95F36F" modified="2006-02-21" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M129</accession>
    <accession>A0A6I8UCB6</accession>
    <name>Q2M129_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Probable cytochrome P450 316a1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Cyp316a1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353244.1">
        <property type="nucleotide sequence ID" value="XM_001353208.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M129"/>
    <dbReference type="FunCoup" id="Q2M129">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M129"/>
    <dbReference type="GeneID" id="4812901"/>
    <dbReference type="KEGG" id="dpo:4812901"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_4_1_1"/>
    <dbReference type="InParanoid" id="Q2M129"/>
    <dbReference type="OMA" id="ANRFNYW"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081136">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2M129">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF189">
        <property type="entry name" value="CYTOCHROME P450 4C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_001353244.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="56504" checksum="4B53B26C41D52DD1" modified="2006-02-21" version="1">MILTTTFLCFCVASAINYFRTRRQRSLIKNLGGPFSWPLMGAMHKLLFLKPQNFFQRSHEYLAKYGAVTRLWVFHRLFMPLADLELAKQVLQSEQHLETGYELMRHWLGGGVLMCDKNQWTQRHGLIGSIFNKGNMLPLTELCRQQAVLLQLTLADHAEQRDVFDAWTIVAPAVRDLMVLTTCGVRPSEGYSEAFTCLTEIYRKRFLSLQSVNRFTFWVSSPLLRRRQAQLIKQLNDEHKEHIDRFRRLQKQEEQLKAGNVVVDRIDPLPAQWHQSLLEILLAGNHNPEISDDDICAELNTCNYLGYLLCSSALCFSLVTISRHPAVQQRCLEELRKAQREDQSWDVRRLPYLEAVLLETLRLYPPQVIVGRHLTEDMPYTHSEVGDGTLPFGAEIYVNLFEMQRNEDRYLNASHFRAERFLESPPEIYSFSMGPRCCPAKQFSMVLLKTLIAPILSNFELLPYGDAPRLDLRLSLGSSNGFQLALKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NTW2</accession>
    <accession>A0A6I8V935</accession>
    <name>A0A0R3NTW2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC26531978</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015036352.1">
        <property type="nucleotide sequence ID" value="XM_015180866.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NTW2"/>
    <dbReference type="GeneID" id="26531978"/>
    <dbReference type="KEGG" id="dpo:26531978"/>
    <dbReference type="InParanoid" id="A0A0R3NTW2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0270965">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029277">
        <property type="entry name" value="SVWC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15430">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01318">
        <property type="entry name" value="SVWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Single" evidence="3">
        <location>
            <begin position="43"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015036352.1"/>
        </source>
    </evidence>
    <sequence length="115" mass="12770" checksum="58F163DBCFD1E660" modified="2016-01-20" version="1">MFSLKVLIFFGLLFASKATKSENFLNFDFSLNSYVHPAYPGRCVIDMEPALITLELGQSVSLPNLPCVSLLCVGDGYGALYKCNHSSPPESCRYGEFLDLNVDFPECCKRKVVCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3NZU4</accession>
    <accession>A0A6I8VHP2</accession>
    <name>A0A0R3NZU4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Troponin T, skeletal muscle isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">up</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015041599.1">
        <property type="nucleotide sequence ID" value="XM_015186113.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3NZU4"/>
    <dbReference type="SMR" id="A0A0R3NZU4"/>
    <dbReference type="FunCoup" id="A0A0R3NZU4">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3NZU4"/>
    <dbReference type="GeneID" id="6901341"/>
    <dbReference type="InParanoid" id="A0A0R3NZU4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0265248">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A0R3NZU4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000003">
        <property type="entry name" value="Troponin T isoform 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF1">
        <property type="entry name" value="TROPONIN T, SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="240"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="303"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="331"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015041599.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="47514" checksum="B76D99022697D290" modified="2016-01-20" version="1">MSDDEEYTSSEEEEVVEETREETKPPQTPAEGEGDPEFIKRQDQKRSDLDEQLKEYISEWRKQRSKEEDELKKLKEKQAKRKISRAEEEQKMAQRKKEEEERRVREAEEKKQREIEEKRMRLEEAEKKRQAMLQAMKDKDKKGPNFTIAKKDAGVLGLSSAAMERNKTKEQLEEEKKISLSFRIKPLAIEGFGEAKLREKAQELWELIVKLETEKYDLEERQKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKYPPKIQVASKYERRVDTRSYDDKKKLFEGGYNTVYAETLEKTWQERQERFTQRTKSKLPKWFGERPGKKAGEPETPEGEEDAKADEDIVEDDEEVEEEVVEEEDEEAEEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P3R2</accession>
    <accession>A0A6I8VKJ5</accession>
    <name>A0A0R3P3R2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC26532281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015042875.1">
        <property type="nucleotide sequence ID" value="XM_015187389.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P3R2"/>
    <dbReference type="GeneID" id="26532281"/>
    <dbReference type="KEGG" id="dpo:26532281"/>
    <dbReference type="InParanoid" id="A0A0R3P3R2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0271268">
        <property type="expression patterns" value="Expressed in male reproductive system"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_015042875.1"/>
        </source>
    </evidence>
    <sequence length="121" mass="13593" checksum="6AF656291370AF44" modified="2016-01-20" version="1">MCKCCRDCQLSVAMKKFAIWNVIFGLLSMDWSYRLMTTSGDYFFTAGCVHMSGAVLLIISGILIRVGIFKKKKAVYCVGLCLSNVGPCIMSYLIFPIVIQVVLLIQGMGLYKKWNEEIVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UCQ1</accession>
    <accession>Q2M0X1</accession>
    <name>A0A6I8UCQ1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Peritrophin-48</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4813810</name>
        <name evidence="11" type="synonym">LOC117185410</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10 11">MV-25-SWS-2005</strain>
            <strain evidence="10">MV2-25</strain>
            <tissue evidence="10 11">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353304.3">
        <property type="nucleotide sequence ID" value="XM_001353268.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033241554.1">
        <property type="nucleotide sequence ID" value="XM_033385663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UCQ1"/>
    <dbReference type="GeneID" id="4813810"/>
    <dbReference type="KEGG" id="dpo:117185410"/>
    <dbReference type="KEGG" id="dpo:4813810"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF106">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044634428" evidence="7">
        <location>
            <begin position="26"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="30"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="88"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="147"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="213"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="8">
        <location>
            <begin position="274"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353304.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033241554.1"/>
        </source>
    </evidence>
    <sequence length="375" mass="39596" checksum="807E1FFA659285BA" modified="2020-08-12" version="1">MPHTSNMNAGIFVILGLALVPLGSASFDPSVICTLVVNGTKINDPRACNAWIECVDGKPVAGTCGADLFYDRESEECVASDSIKCVSSDPCASVLNGFTADPYSCSSYYYCLNGKGTKGTCPTGMNYSAGTEDCIRNFPCQVKMDPDNYCNILPDGVFIKDTTNCNGWQMCWDGQVLNGTCPGTFYFSAGSARCDYPQNVECDFTVAPDIDEMGVCAHAGDFVSDNISCNGYYYCHELEDGQMALEHAVCSDGRFFLAVDGGACVPRYKAPCAYDRCVGLGNTTIQLASESDDGCRGFSLCQDGASIGAGTCPGEEYFDEVTQRCTTQVISYPACQLSAAASTKVVSQTAMNDDTDGTSPSPSPSASASASTEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UD06</accession>
    <name>A0A6I8UD06_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger MYND domain-containing protein 10 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Zmynd10</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353504.3">
        <property type="nucleotide sequence ID" value="XM_001353468.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UD06"/>
    <dbReference type="FunCoup" id="A0A6I8UD06">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812783"/>
    <dbReference type="InParanoid" id="A0A6I8UD06"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UD06">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052298">
        <property type="entry name" value="ZMYND10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244:SF7">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="421"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353504.3"/>
        </source>
    </evidence>
    <sequence length="461" mass="52557" checksum="A20103E9097CE68E" modified="2020-08-12" version="1">MVNFVYPEELYFFVESIRAFEVRDVGSSKWLEVHEMVIKLSQQAAYEASQNREEEVKEFLISRDKLRLLIHEAFCVNLWKTRVLPHLLEIDPNPQATFLIYTVMYHEAALVALLDMCLYHPSGCETLQESVLDLIDYCGHSVAQVIGLVSMGYHENESKVDVDEAVLTELERQKRNFVYKIGLRSISILNYLADNVSLFHLSAARRMLFTHDIPWLMVDVLCFRPWQRKTSKGLQKFIDEKWTNVEDAAKIVKPEAQAWFCVHQLLLNPQVMENYELNEARCKQLSKLLGLMHETLLDQLPVLIGLKQFLSRLTLSGNTTAKLQPLLLEDLPQIQNDLTKEVDREGGFYQIAQAQDSLFLSRDKKQICDLASRLSAAYGTELLCELEQNMADLNVQEGNPVSQPDSGAGGDTASGEQIKKCATCLVLAKKMCANCKKVHYCSRECQLKDWANHKTACLDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE55</accession>
    <name>A0A6I8UE55_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Gram-negative bacteria-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4813434</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the insect beta-1,3-glucan binding protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353992.4">
        <property type="nucleotide sequence ID" value="XM_001353956.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE55"/>
    <dbReference type="FunCoup" id="A0A6I8UE55">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813434"/>
    <dbReference type="InParanoid" id="A0A6I8UE55"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UE55">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02179">
        <property type="entry name" value="GH16_beta_GRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2140">
        <property type="entry name" value="Beta-1,3-glucan-recognition protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031756">
        <property type="entry name" value="BGBP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043030">
        <property type="entry name" value="BGBP_N_sf"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035806">
        <property type="entry name" value="GH16_GRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF60">
        <property type="entry name" value="GRAM-NEGATIVE BACTERIA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15886">
        <property type="entry name" value="CBM39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51969">
        <property type="entry name" value="CBM39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026259972" evidence="6">
        <location>
            <begin position="24"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="CBM39" evidence="8">
        <location>
            <begin position="24"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="7">
        <location>
            <begin position="182"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353992.4"/>
        </source>
    </evidence>
    <sequence length="464" mass="53546" checksum="81A2D359E33E0E14" modified="2020-08-12" version="1">MCWKTLLCCSLWLLLLKSNENLAFKIPSIDFEMLHELGFEVSIPDEPGMQRVFYMLQIDDNCPALMDYITESNNGSWISRQKTSLQNNDKLQISVLVQYNDEIYEKSETRVIINARLLTTRDGRSQSITYPNGEGECHAYLSPQKQAKRCKPAQSIVSSSRQTCQGELLFEDNFSEAQLNRSTWKHDIRQRMYHVEEELVAFDDAPRNSYVKEGYLHIVPIVASEVVEGSFKLGDRCTAVESPEQECNIAQGSFYSIKPPVFSAQIHTRDSFSFKYGKIVVRAKLPKGDWLFPYVMLQPVSTYAETHYAKQLRVAYARGNSHLQSNQQEDISGSHLYGGMVVWPHGRAMQYLKDRLSKAHYGDSFHNYTMIWQRDKITLMVDEEIYGELYEGLSFFNEKCFIIFGVTVGGFLNFDDSVLAKDVKPYLNREPRAALSFWQQRDTWAPTWGKHSSMIIDYVRVYAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UEU4</accession>
    <name>A0A6I8UEU4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4814056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354286.3">
        <property type="nucleotide sequence ID" value="XM_001354250.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UEU4"/>
    <dbReference type="KEGG" id="dpo:4814056"/>
    <dbReference type="InParanoid" id="A0A6I8UEU4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026067391" evidence="1">
        <location>
            <begin position="23"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001354286.3"/>
        </source>
    </evidence>
    <sequence length="209" mass="22170" checksum="8E852B96923BC9C4" modified="2020-08-12" version="1">MQIHQAVLGATVLGMLLAFSCSLPVLDISSGIDNATIEELRASVNDTPKNLYVVKAVVYEIGILTEVGENDTSFESQERVDLTFYDTHSNKSHIDLGNIPLPIQTNVTGQVLTGIAPVNLGAFSSPQELLETLPLTGSIVNITHSDTAFYQLSRSNTSAADKPQILDKDALAQLTHAAQLFPPSSSIGQSLPVNPAADNEVALADQATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UIQ0</accession>
    <name>A0A6I8UIQ0_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">10-formyltetrahydrofolate dehydrogenase</fullName>
            <ecNumber evidence="9">1.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC4816568</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6R)-10-formyltetrahydrofolate + NADP(+) + H2O = (6S)-5,6,7,8-tetrahydrofolate + CO2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10180"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="1.5.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10181"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">In the N-terminal section; belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.6" evidence="9"/>
    <dbReference type="RefSeq" id="XP_001356099.2">
        <property type="nucleotide sequence ID" value="XM_001356063.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UIQ0"/>
    <dbReference type="FunCoup" id="A0A6I8UIQ0">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816568"/>
    <dbReference type="InParanoid" id="A0A6I8UIQ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016155">
        <property type="term" value="F:formyltetrahydrofolate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009258">
        <property type="term" value="P:10-formyltetrahydrofolate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07140">
        <property type="entry name" value="ALDH_F1L_FTFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.25.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000050">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000008">
        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.25.10">
        <property type="entry name" value="Formyl transferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011407">
        <property type="entry name" value="10_FTHF_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037022">
        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036489">
        <property type="entry name" value="10-FTHFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 12" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="16">
        <location>
            <begin position="329"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="683"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="683"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="717"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="93"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="607"/>
            <end position="610"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="640"/>
            <end position="645"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="660"/>
            <end position="661"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="767"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="815"/>
            <end position="817"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="13">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001356099.2"/>
        </source>
    </evidence>
    <sequence length="913" mass="99825" checksum="A93B1F9EB1BB0BA5" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UJM7</accession>
    <name>A0A6I8UJM7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transcription factor Sp2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cbt</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001356430.4">
        <property type="nucleotide sequence ID" value="XM_001356394.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UJM7"/>
    <dbReference type="FunCoup" id="A0A6I8UJM7">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817379"/>
    <dbReference type="InParanoid" id="A0A6I8UJM7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001110">
        <property type="entry name" value="Krueppel factor 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002639">
        <property type="entry name" value="Kruppel-Like Factor (Zinc finger protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF174">
        <property type="entry name" value="CABUT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="280"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="310"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="48"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="120"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001356430.4"/>
        </source>
    </evidence>
    <sequence length="452" mass="49034" checksum="B9B83CE95C6FC63F" modified="2020-08-12" version="1">MDMNMDTLLLPSPPATPPLRENKLENVAKDEQQVNENLLKAKLKLVAQKSQKNGGGIITPNPSDTEDEAPEMDEVPSKKPRLEQPAMTMTPPPDQQKPEEEEESKSDDEPKRVSVIMRVNSSGAVSSSQDDNSSSSSCSSSCTTATSSTVSPAMEDDYPEANVWRNLKFKMNRKRAAEVALPQPPAPVQAPAPAPTPEPAEEPQTEKTEPIAFVPPSSSILIAPPTSPSIACASQLLLLSTVAAQQCPNPCPPPSSQESPAEKHQRITAAQAAATRSRIYECTFPDCGKNYFKSSHLKAHQRVHTGERPFVCKWENCDKRFSRSDELSRHKRTHTGEKKFQCGVCQKKFMRSDHLSKHVKRHNKDKANGVNRNVSVASASSAAVAALCDPTLHLRAIAPAAAVSSSVQTSLPVTQSSPATLQVYSAQDLLRLQQQSSGFSFSGVGTLLQAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK07</accession>
    <name>A0A6I8UK07_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC4817380</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356763.3">
        <property type="nucleotide sequence ID" value="XM_001356727.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK07"/>
    <dbReference type="FunCoup" id="A0A6I8UK07">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817380"/>
    <dbReference type="InParanoid" id="A0A6I8UK07"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UK07">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000021">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026103648" description="Lipase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="10">
        <location>
            <begin position="41"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_001356763.3"/>
        </source>
    </evidence>
    <sequence length="410" mass="45922" checksum="C114D80274E5E35D" modified="2020-08-12" version="1">MLKELLLAALAITAVLAVAKDTDDFDPFIDIPFKRLRTSAERIESEGYPAESHYVETPDGYVLNLFRIPHSPRLNNGQLQRPAVLIMHGLFSCSDCFLLNGPDDALAYNLADAGYDVWLGNARGNLYSRNNTRLNVRHPYFWKFSWHEIGAIDLPAMIDYILELTQERALHYVGHSQGCTSFFVMGSFRPEYNAKIKTAHMLAPSVFMGNTTEGLIVATAPVLGEPGLGSALLENQVLLPYNDFLQRFLDTTCSNQPLTLSYCKTLAILWGGPDIGNLNRTLLPQITETHPAGVSSNQAIHYIQSYTSNQFRLYDWGSRKNLAYYGVAEPPSYDLTQITAELYLYYGLSDGSANKDDVARLPELLPNLALLHEVPEPTWGHLDFIFAVKVKSLINDLVLDHTKAYEERGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UK72</accession>
    <name>A0A6I8UK72_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein Spindly</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Spindly</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356697.2">
        <property type="nucleotide sequence ID" value="XM_001356661.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UK72"/>
    <dbReference type="FunCoup" id="A0A6I8UK72">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4816829"/>
    <dbReference type="InParanoid" id="A0A6I8UK72"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="526"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="610"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="666"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="717"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="298"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="556"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="675"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="717"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356697.2"/>
        </source>
    </evidence>
    <sequence length="774" mass="87861" checksum="5B2CB4EE15F630D6" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UL55</accession>
    <name>A0A6I8UL55_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mitochondrial uncoupling protein 4C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4817947</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357206.2">
        <property type="nucleotide sequence ID" value="XM_001357170.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UL55"/>
    <dbReference type="KEGG" id="dpo:4817947"/>
    <dbReference type="InParanoid" id="A0A6I8UL55"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000062">
        <property type="entry name" value="mitochondrial uncoupling protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="38"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="137"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="238"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001357206.2"/>
        </source>
    </evidence>
    <sequence length="335" mass="37552" checksum="759CF77D0EA075C3" modified="2020-08-12" version="1">MDKDPQKHSILKSNKMDRRPQLPPVNVAEPLTVRLVLQLYFNTFLGANFAESFVFPLDVAKTRMQVEGEEAKKTGAKVPNALATLKSMVKNEGPKTLYAGFSAMVARNLIFNSMRVVLYDVFRRQFIRVDENKKETLSVSSALMCGFVAGCIAQALANPFDIVKVRMQTEGRRRQLGHKPRVSNMFNAFTGIYREGGLPSMWRGITPSCLRACLMTAGDVGTYDLSKRFFKNLLELDDGLRLRFLSSMCAGLAASVLSNPADVIKSRVMNQQTDAAGKNLTYKNSMDCLVKTVREEGILTLYKGLLPCWFRLGPFSILFWLSVEFLRDLEGQTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULE1</accession>
    <name>A0A6I8ULE1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4817926</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001357236.4">
        <property type="nucleotide sequence ID" value="XM_001357200.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULE1"/>
    <dbReference type="FunCoup" id="A0A6I8ULE1">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817926"/>
    <dbReference type="InParanoid" id="A0A6I8ULE1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001357236.4"/>
        </source>
    </evidence>
    <sequence length="150" mass="17060" checksum="6DEDA66D1B8AEE41" modified="2020-08-12" version="1">MGSYRNIYLTMSHLRFQLFVLYSFCFHSSLHQLVPVPDSDLMAGNELLMASAEDDLEWTQADWQLVIPWLLQLQQVRLCVAVSIFDQQLVYGTPCAGLLAQGPLMASCDIGHIEDLSVTMRDAFGPMLFDTMQRCRPGLELFGVRCRRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULQ0</accession>
    <name>A0A6I8ULQ0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Malectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4818193</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357504.4">
        <property type="nucleotide sequence ID" value="XM_001357468.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULQ0"/>
    <dbReference type="FunCoup" id="A0A6I8ULQ0">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4818193"/>
    <dbReference type="InParanoid" id="A0A6I8ULQ0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081634">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8ULQ0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039155">
        <property type="entry name" value="MLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460:SF0">
        <property type="entry name" value="MALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin" evidence="12">
        <location>
            <begin position="68"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="287"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001357504.4"/>
        </source>
    </evidence>
    <sequence length="379" mass="42757" checksum="26C40E825A40ED9B" modified="2020-08-12" version="1">MKKKLKLTTTKTTENMCNVACNGGDYLRASSNSSTGCRGGQPLQPVVVLLLLIGCCCLSMAAADPLKVVYAVNAGGEEHTDRNGITYEADPLKNVGIASDYGKHLLMIGRTHEHDEMLYRTERYHTTTFGYDFPTDGDGDYALIMKFCEVYFDAPGRKVFDVLLNRKHTVIRQLDIYEQVGRGSAHDEIVYFKINNGRLHYLGEVSDVRNGQLRLDFIKGALDNPKINAFALLKGDISQIPTSEASTRIGPEKPNQGQQERVVRTQRDEIDEDDEDLDDLDELEEEDMLEQHAPPPPQQQQQHQHQNHHHQQQPPQQPQQQQQQQQRQEQSQAQTPDSKRAFSGPRQPNPYSMDDSSILLPVFIAIGAFIPLLFCLCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULT5</accession>
    <name>A0A6I8ULT5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">LOW QUALITY PROTEIN: pituitary homeobox homolog Ptx1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Ptx1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357586.4">
        <property type="nucleotide sequence ID" value="XM_001357549.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8ULT5">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800323"/>
    <dbReference type="InParanoid" id="A0A6I8ULT5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000031">
        <property type="entry name" value="Homeobox protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882:SF3">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="347"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="11">
        <location>
            <begin position="540"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="349"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="153"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="289"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="629"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="153"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="328"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="632"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="660"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="691"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="707"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001357586.4"/>
        </source>
    </evidence>
    <sequence length="717" mass="75393" checksum="B03E70C395C11A53" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8ULX9</accession>
    <name>A0A6I8ULX9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Solute carrier family 25 member 51</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC4800248</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="14">MV-25-SWS-2005</strain>
            <tissue evidence="14">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357640.2">
        <property type="nucleotide sequence ID" value="XM_001357603.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8ULX9"/>
    <dbReference type="FunCoup" id="A0A6I8ULX9">
        <property type="interactions" value="1436"/>
    </dbReference>
    <dbReference type="GeneID" id="4800248"/>
    <dbReference type="KEGG" id="dpo:4800248"/>
    <dbReference type="InParanoid" id="A0A6I8ULX9"/>
    <dbReference type="OMA" id="GCAFNTG"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0080699">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052465">
        <property type="entry name" value="Mito_NAD+_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46131">
        <property type="entry name" value="SD08549P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46131:SF1">
        <property type="entry name" value="SD08549P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="47"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="135"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="229"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_001357640.2"/>
        </source>
    </evidence>
    <sequence length="336" mass="37889" checksum="F343EDCB4CA33D44" modified="2020-08-12" version="1">MKDDDDSEAQNAYATKTTTRRAPPPTALKLGVSSSHASIFTERFFGAFQWEEFACGCGAAFVNISATYPIYKMIFRQMLHGVPIKSAFGQLRHEGLSFLYRGMLPPLAQKTISLSIMFGVFDGTRRYLVEDYRLNDYGAKVIAGVVAGSAESILLPFERVQTLLADSKFHQHFSNTSNAFRYVVAHHGYRELYRGIEPVFWRNGLSNAFFFVLREEASARLPKRKSVSSRTAQEFIAGAVIGASISTIFYPLNVIKVSLQSDMGHRSEGSWHACKRIYRERDSRIGNFYRGCAFNTARSFISWGIMNTAYENLKKLMHEQPQIPQMPHTSPISSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UN73</accession>
    <name>A0A6I8UN73_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phenoloxidase-activating factor 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4800893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358087.2">
        <property type="nucleotide sequence ID" value="XM_001358050.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UN73"/>
    <dbReference type="FunCoup" id="A0A6I8UN73">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4800893"/>
    <dbReference type="InParanoid" id="A0A6I8UN73"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258:SF140">
        <property type="entry name" value="BCDNA.GH08420-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24258">
        <property type="entry name" value="SERINE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026039387" evidence="1">
        <location>
            <begin position="21"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="37"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001358087.2"/>
        </source>
    </evidence>
    <sequence length="291" mass="32391" checksum="BEF34416AC4A1ACF" modified="2020-08-12" version="1">MHWGTAFITLLLATWTGVLSAQLEDTECGYFDEHQFLKQGSFAIPTEHQWVARIVFGKGFEGKIQNDGCLGALISKRNVLAPAHCFVQYNGEAEAFSVHLGVWNKSSAISQPSCDRDGFCVVPAQEIKLAEIAIHPEYDPLTLKNSLAVLTLQRDAKLNPNVMPVCMPPPQLVNETLVGQTFIVAGLRVKEDLKLKTWVNTLSRGFCQSKVSSIVTSSTTVCGYQAKPEGYYIGAPLVGMQVKGDVTQNFYLVGLMIDWRWENNRILSSFLAIRHYLDFIYQNANSLIVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPC2</accession>
    <name>A0A6I8UPC2_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RPA1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">Component of the heterotrimeric canonical replication protein A complex (RPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001358758.3">
        <property type="nucleotide sequence ID" value="XM_001358721.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPC2"/>
    <dbReference type="FunCoup" id="A0A6I8UPC2">
        <property type="interactions" value="2548"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801711"/>
    <dbReference type="InParanoid" id="A0A6I8UPC2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04477">
        <property type="entry name" value="RPA1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000117">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 11 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor-A protein 1 N-terminal" evidence="14">
        <location>
            <begin position="6"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="13">
        <location>
            <begin position="178"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="16">
        <location>
            <begin position="288"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="15">
        <location>
            <begin position="443"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_001358758.3"/>
        </source>
    </evidence>
    <sequence length="603" mass="66779" checksum="3410ADCD53DA647A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPM7</accession>
    <name>A0A6I8UPM7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleolar pre-ribosomal-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4801669</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001358721.3">
        <property type="nucleotide sequence ID" value="XM_001358684.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8UPM7">
        <property type="interactions" value="661"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801669"/>
    <dbReference type="InParanoid" id="A0A6I8UPM7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039844">
        <property type="entry name" value="URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032436">
        <property type="entry name" value="URB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021714">
        <property type="entry name" value="URB1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500:SF0">
        <property type="entry name" value="NUCLEOLAR PRE-RIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500">
        <property type="entry name" value="NUCLEOLAR PRERIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16201">
        <property type="entry name" value="NopRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11707">
        <property type="entry name" value="Npa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="URB1 N-terminal" evidence="2">
        <location>
            <begin position="179"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 C-terminal" evidence="3">
        <location>
            <begin position="1702"/>
            <end position="1904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001358721.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2070" mass="236796" checksum="8FD849FA5651EBE2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UPN6</accession>
    <name>A0A6I8UPN6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pyruvate kinase</fullName>
            <ecNumber evidence="15">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4801685</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:18159"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="15"/>
    <dbReference type="RefSeq" id="XP_001358734.3">
        <property type="nucleotide sequence ID" value="XM_001358697.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UPN6"/>
    <dbReference type="FunCoup" id="A0A6I8UPN6">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4801685"/>
    <dbReference type="InParanoid" id="A0A6I8UPN6"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000025">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 15 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 19" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="16">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="17">
        <location>
            <begin position="344"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001358734.3"/>
        </source>
    </evidence>
    <sequence length="502" mass="54502" checksum="66B2DFA0598C1890" modified="2020-08-12" version="1">MRVVRLNFSHGSHEYHSQTIKAARKAIDMYAKQTGVFKPVAIALDTKGPEIRTGKIEGGDTSEIELKQGDSIKLSTNKELQKKCNAERVYVDYKKLPSIIKTGDRVYIDDGLIALKVKQVAGDEVTCEVENGGKLGSHKGVNLPGVAVDLPAVSEKDKADLKFGVEQKVDMVFASFIRDAQALAEIRNVLGPEGKNIKIISKIENQQGMANIDSIITASDGIMVARGDLGIEILTDEVPLAQKAIIAKCNQVGKPVICATQMLDSMIGKPRPTRAEASDVANAIFDGADCVMLSGETAKGKYPVECIKCMAKICSKVEAVLWYESIQNNVRSVIRMAPANQISAVSMSIVEAATTAHAKAIIVASPCSMIPQLVSQMRPACPIVMLIGCKSQARQSVIFRGIYPVIIDEMATGCTDFGEILKMGIKLLPKLDIMEPGKKVTVVIVDAMRADKISFGLFSVRQQTKAEMEKQAAEGKKKQPKKCIKLLGDEIKNDQAKIKQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQ26</accession>
    <name>A0A6I8UQ26_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein Saysd1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Saysd1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359097.2">
        <property type="nucleotide sequence ID" value="XM_001359060.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQ26"/>
    <dbReference type="FunCoup" id="A0A6I8UQ26">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802116"/>
    <dbReference type="InParanoid" id="A0A6I8UQ26"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039159">
        <property type="entry name" value="SAYSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019387">
        <property type="entry name" value="SAYSvFN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13527">
        <property type="entry name" value="SAYSVFN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13527:SF0">
        <property type="entry name" value="SAYSVFN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10260">
        <property type="entry name" value="SAYSvFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="SAYSvFN" evidence="2">
        <location>
            <begin position="101"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001359097.2"/>
        </source>
    </evidence>
    <sequence length="177" mass="20603" checksum="27E9C8C2768F9AC1" modified="2020-08-12" version="1">MADFQEQLKQYRLKKRRKKTIENLKDKFYRFLMFGTGSPKETTIDLQQPSKNHPELFEENSHDGSLSSSANEFVEESSIMPETEDHSGGNVLKYTLWTVYLLFWITLYVIAIELKFGLVYLMFSALFGIYFNTRTGPKKQNEISAYSVFNKNFESIDGTLKAEQFEREIRYGSGSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UQR5</accession>
    <name>A0A6I8UQR5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA transcription, translation and transport factor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4802387</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359315.2">
        <property type="nucleotide sequence ID" value="XM_001359278.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UQR5"/>
    <dbReference type="FunCoup" id="A0A6I8UQR5">
        <property type="interactions" value="1118"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802387"/>
    <dbReference type="InParanoid" id="A0A6I8UQR5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019265">
        <property type="entry name" value="RTRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15924">
        <property type="entry name" value="CLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10036">
        <property type="entry name" value="RLL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001359315.2"/>
        </source>
    </evidence>
    <sequence length="248" mass="28058" checksum="24D826F1E9472E3D" modified="2020-08-12" version="1">MLKKKLEALGYPTPSDLALSNRKDFASTILWLEDQKIRLYAIEDREILRNVDNAQMWEDGYLKYCADLNMPSLGTQNEQLAWILGHAIRLEFLDDPAQYESINSKQPLPKINGSQPHQQKVQVIFEGKMNVDDKEFIAGVRTLAGQLNVPHHPNHLLQLEAVARVVHERLSPAARGRQPITMTPFPFDKGNDVVSANDPALDLPMRILRLLQIQSLRQLQTHINETIVSVQNLTANPKTDTKLGKVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UR32</accession>
    <name>A0A6I8UR32_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">18S rRNA (guanine-N(7))-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Bud site selection protein 23 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">rRNA methyltransferase and ribosome maturation factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4802719</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(7) position of a guanine in 18S rRNA. Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity. Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity. Locus-specific steroid receptor coactivator. Potentiates transactivation by glucocorticoid (NR3C1), mineralocorticoid (NR3C2), androgen (AR) and progesterone (PGR) receptors. Required for the maintenance of open chromatin at the TSC22D3/GILZ locus to facilitate NR3C1 loading on the response elements. Required for maintenance of dimethylation on histone H3 'Lys-79' (H3K79me2), although direct histone methyltransferase activity is not observed in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a guanosine in 18S rRNA + S-adenosyl-L-methionine = an N(7)-methylguanosine in 18S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:54584"/>
            <dbReference type="Rhea" id="RHEA-COMP:13937"/>
            <dbReference type="Rhea" id="RHEA-COMP:13938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer with TRMT112; this heterodimerization is necessary for the metabolic stability and activity of the catalytic subunit BUD23. Interacts with GRIP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. BUD23/WBSCR22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359583.2">
        <property type="nucleotide sequence ID" value="XM_001359546.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UR32"/>
    <dbReference type="FunCoup" id="A0A6I8UR32">
        <property type="interactions" value="2003"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802719"/>
    <dbReference type="InParanoid" id="A0A6I8UR32"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000017">
        <property type="entry name" value="probable 18S rRNA (Guanine-N(7))-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039769">
        <property type="entry name" value="Bud23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022238">
        <property type="entry name" value="Bud23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734:SF0">
        <property type="entry name" value="18S RRNA (GUANINE-N(7))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12589">
        <property type="entry name" value="WBS_methylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="17">
        <location>
            <begin position="55"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23 C-terminal" evidence="16">
        <location>
            <begin position="202"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001359583.2"/>
        </source>
    </evidence>
    <sequence length="277" mass="31266" checksum="A59678E3026F9475" modified="2020-08-12" version="1">MARRPEHSAPPEIFYNDNEAKKYSTNTRIIEIQVEMAERALELLAFPDDDESRLILDIGCGSGLSGSVLEDSDHMWIGIDISKSMLDIAVEREVAGDVILGDMGEGMPFKPGTFDGAISISALQWLCNADKTYHNPHKRLHKFFSTLFSCLTRTARAVFQFYPENSDQIEMVTSQAMKAGFYGGLVVDYPNSAKAKKYYLVLMTGGAAELPKALGASPEEERRVNYIKKRDACREARGKAPKKSRDWILAKKERRRRQGLETRPDTKYTARKRTGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URA0</accession>
    <name>A0A6I8URA0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein js</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">js</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001359455.3">
        <property type="nucleotide sequence ID" value="XM_001359418.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8URA0"/>
    <dbReference type="FunCoup" id="A0A6I8URA0">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4802557"/>
    <dbReference type="InParanoid" id="A0A6I8URA0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052976">
        <property type="entry name" value="Scoloptoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933">
        <property type="entry name" value="FI18007P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22933:SF44">
        <property type="entry name" value="RE15157P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026250710" evidence="2">
        <location>
            <begin position="29"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="46"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359455.3"/>
        </source>
    </evidence>
    <sequence length="658" mass="72475" checksum="AB2781D3701B6D16" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URE0</accession>
    <name>A0A6I8URE0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Caprin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC4802875</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
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            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophila</taxon>
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        </lineage>
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        <citation type="submission" date="2024-06" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">MV2-25</strain>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="5">MV-25-SWS-2005</strain>
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        <property type="entry name" value="AGAP003037-PA"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026154167" evidence="3">
        <location>
            <begin position="19"/>
            <end position="378"/>
        </location>
    </feature>
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            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_001359706.3"/>
        </source>
    </evidence>
    <sequence length="378" mass="42557" checksum="3176489A1E44C24F" modified="2020-08-12" version="1">MQLPLIALVCSALALAQAQNYPQRLNIPGTLVSPGQVPHRQAVLRVRRPGQSLRQTNAILPAVTQRISSPIEERPVTEAAEDEQPEPYLPNLLREQQLAHSQQSQFQQAAAAFLNGQQAIDSPSPVQQFLQQNEDSQPAAILPAPPRFAERPSIPAPSNRPAAFNDFGIGRFENSQRFGLEQRPTPTAAAPPPPPPQQQQPQRVAVIRTRPAAALRPEPPAQQQAPRPRPKPVQPRPIIDQNQLQDERSEREQQHQQQQRSRRPVAQTLRKWRDENEDGSITWGYENDDGSFKEELIGTDCITKGTYGYIDPDGNKREYNYETGIRCDPNARDNDEELQENGFINYEENRAVLPNGLEIDMTQLGKKKSKRPSSIYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URE8</accession>
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        <submittedName>
            <fullName evidence="12">Mitogen-activated protein kinase kinase kinase 15</fullName>
        </submittedName>
    </protein>
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        <name evidence="12" type="primary">Ask1</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
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                <consortium name="RefSeq"/>
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        </citation>
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            <strain evidence="12">MV-25-SWS-2005</strain>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="581"/>
            <end position="839"/>
        </location>
    </feature>
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        <location>
            <begin position="871"/>
            <end position="891"/>
        </location>
    </feature>
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        <location>
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        <location>
            <begin position="871"/>
            <end position="887"/>
        </location>
    </feature>
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        <location>
            <begin position="1090"/>
            <end position="1102"/>
        </location>
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            <begin position="1326"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="610"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_001359730.3"/>
        </source>
    </evidence>
    <sequence length="1357" mass="154105" checksum="63DFE209FE64BF0E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8URQ1</accession>
    <name>A0A6I8URQ1_DROPS</name>
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        <submittedName>
            <fullName evidence="13">Cytochrome P450 315a1, mitochondrial isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">sad</name>
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    <organism evidence="12 13">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="13">MV-25-SWS-2005</strain>
            <tissue evidence="13">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8URQ1">
        <property type="expression patterns" value="baseline"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cyt_P450"/>
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        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF120">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026016059" evidence="11">
        <location>
            <begin position="19"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_001359643.3"/>
        </source>
    </evidence>
    <sequence length="517" mass="59028" checksum="FE0B4F410ED8B06D" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US04</accession>
    <name>A0A6I8US04_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-1-tetrahydrofolate synthase, cytoplasmic</fullName>
            <ecNumber evidence="8">1.5.1.5</ecNumber>
            <ecNumber evidence="7">3.5.4.9</ecNumber>
            <ecNumber evidence="6">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">pug</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="29">MV-25-SWS-2005</strain>
            <tissue evidence="29">Whole body</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="8"/>
    <dbReference type="EC" id="3.5.4.9" evidence="7"/>
    <dbReference type="EC" id="6.3.4.3" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001360009.3">
        <property type="nucleotide sequence ID" value="XM_001359972.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US04"/>
    <dbReference type="FunCoup" id="A0A6I8US04">
        <property type="interactions" value="1499"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803236"/>
    <dbReference type="InParanoid" id="A0A6I8US04"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8US04">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.770:FF:000001">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000556">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001522">
        <property type="entry name" value="Probable MIS1-C1-tetrahydrofolate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF5">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="21" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="22" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="26">
        <location>
            <begin position="43"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="27">
        <location>
            <begin position="181"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00036357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_001360009.3"/>
        </source>
    </evidence>
    <sequence length="970" mass="104125" checksum="CAB505FFE65324C0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8US93</accession>
    <name>A0A6I8US93_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein takeout</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC4803215</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001359991.4">
        <property type="nucleotide sequence ID" value="XM_001359954.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8US93"/>
    <dbReference type="FunCoup" id="A0A6I8US93">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4803215"/>
    <dbReference type="InParanoid" id="A0A6I8US93"/>
    <dbReference type="OMA" id="INENWRE"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0071292">
        <property type="expression patterns" value="Expressed in reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.30:FF:000001">
        <property type="entry name" value="Takeout-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.30">
        <property type="entry name" value="Haemolymph juvenile hormone binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010562">
        <property type="entry name" value="Haemolymph_juvenile_hormone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038606">
        <property type="entry name" value="To_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008:SF32">
        <property type="entry name" value="CIRCADIAN CLOCK-CONTROLLED PROTEIN DAYWAKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11008">
        <property type="entry name" value="PROTEIN TAKEOUT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06585">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00700">
        <property type="entry name" value="JHBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026204507" evidence="4">
        <location>
            <begin position="25"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_001359991.4"/>
        </source>
    </evidence>
    <sequence length="256" mass="28248" checksum="9E8571F239AD51BE" modified="2020-08-12" version="1">MERNFSLVLLLLPLLSCLTISVRGHASDLPAGIQRCAIKDNECVLNGVNFVLKNHAQSGIKELGLIPLDPLHVTKFTIGRNPHSPVSIDLSFTEVDLLGLSRARAKRLGGFSLDLSNPIEFVMEAPELAFKGPYSVDGKVLILPIVGKGRAEIVLKHCKIHSLITLRPISKGGHHTFAEVTDIKLQVDPSHVSYKLEGLFHGQKDLSENMHILINENWQEIFNELKPSISEAMGLIVKSVLNKTFGKTPLEELFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8USB4</accession>
    <name>A0A6I8USB4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc transporter ZIP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Zip42C.2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001360149.2">
        <property type="nucleotide sequence ID" value="XM_001360112.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8USB4"/>
    <dbReference type="FunCoup" id="A0A6I8USB4">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="GeneID" id="4803434"/>
    <dbReference type="KEGG" id="dpo:4803434"/>
    <dbReference type="InParanoid" id="A0A6I8USB4"/>
    <dbReference type="OMA" id="IFFEIVA"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081767">
        <property type="expression patterns" value="Expressed in adult organism"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF203">
        <property type="entry name" value="FI18611P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001360149.2"/>
        </source>
    </evidence>
    <sequence length="314" mass="34809" checksum="709D71BAFDDF0794" modified="2020-08-12" version="1">MADQQLMVAKIVAIVVLLVVTLIFCFLPYLLDRFYKWTKRAKGNAREFMVVLCLLNFGGGVLIATTFIHMLPEVVELVNALQQCRMLVPTPFGLPEVLLCTGFYLMYFIEETMHFVVRRRQLRKGPAVKVVVEVLEEGQKQEPQAELVVEQQEPEEPNWLRGLGIIVALSLHELFGGMAIGLEMSVDTVWFMCGAIAVHKLVLAFCIGMEIMMAHTRWLIAVIYLLVFSIVTPIGLGIGIAVSETSSANEPSTASGILQSLACGTLIYVIFFEIVAKNHAGWRVFVSSLVGFILMFGLQIAIGEAKGKSHFVCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UUX8</accession>
    <name>A0A6I8UUX8_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC4804781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001361290.3">
        <property type="nucleotide sequence ID" value="XM_001361253.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UUX8"/>
    <dbReference type="FunCoup" id="A0A6I8UUX8">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4804781"/>
    <dbReference type="InParanoid" id="A0A6I8UUX8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051367">
        <property type="entry name" value="mRNA_TranslReg/HistoneTransl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254">
        <property type="entry name" value="EIF4G DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254:SF18">
        <property type="entry name" value="RE28271P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="562"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="602"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="655"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001361290.3"/>
        </source>
    </evidence>
    <sequence length="744" mass="84641" checksum="C89E49221B107B8E" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWR3</accession>
    <name>A0A6I8UWR3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein Ir48c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ir48c</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001361812.2">
        <property type="nucleotide sequence ID" value="XM_001361775.3"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
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        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF41">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026040423" evidence="9">
        <location>
            <begin position="19"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="329"/>
            <end position="348"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="541"/>
            <end position="565"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001361812.2"/>
        </source>
    </evidence>
    <sequence length="574" mass="65323" checksum="3ABF2030D52A3D93" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWS7</accession>
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        </submittedName>
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        <name evidence="5" type="primary">LOC6903329</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_002132210.2">
        <property type="nucleotide sequence ID" value="XM_002132174.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UWS7"/>
    <dbReference type="KEGG" id="dpo:6903329"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="HARBI1_dom"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="198"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_002132210.2"/>
        </source>
    </evidence>
    <sequence length="387" mass="43340" checksum="B4DBE99A0B195843" modified="2020-08-12" version="1">MNVRKTFLLNFFLDDGVEANLLHQAAQLVSLEGTLTQHVAVKETVKEEAEEEDEQENKDSVRKMDDQFKERFRMQRSTFETLLQVVGRAIAGAEQYQPIGSVSLPEKLLYTLMLLGGGISFRDAGEIYSISKSSGHEIFKWVTSAFSALMPCYVQWPKYGAAGVGSSSISKLPGVLGVIEECRIPLKLPIRIDDDNPKQYPALTLQAVCDAQSRFLDVYVDAPNDDQHCILLASPLFEKLIHTQTPLMAEHRHIVGDKTYPLLLNLMTPYVGELTPCHAQYNLAIRLWNAPAERALATLISRFKRLDSLDVSSLELGTTVISAACMLHNFILDSGVEGMEDDILAFDMLPENHKKIPYDEHGLLDLENIMAVEEKRDRLISGFINNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UWT3</accession>
    <name>A0A6I8UWT3_DROPS</name>
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        <submittedName>
            <fullName evidence="12">PDZ and LIM domain protein Zasp isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Zasp52</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_001362080.3">
        <property type="nucleotide sequence ID" value="XM_001362043.4"/>
    </dbReference>
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        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="GeneID" id="4805725"/>
    <dbReference type="KEGG" id="dpo:4805725"/>
    <dbReference type="InParanoid" id="A0A6I8UWT3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075652">
        <property type="expression patterns" value="Expressed in adult organism and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UWT3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09455">
        <property type="entry name" value="LIM1_Enigma_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09461">
        <property type="entry name" value="LIM3_Enigma_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09360">
        <property type="entry name" value="LIM_ALP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23068">
        <property type="entry name" value="PDZ_ZASP52-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000060">
        <property type="entry name" value="Uncharacterized protein, isoform Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000067">
        <property type="entry name" value="Uncharacterized protein, isoform Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000069">
        <property type="entry name" value="Uncharacterized protein, isoform Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000073">
        <property type="entry name" value="Uncharacterized protein, isoform Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000118">
        <property type="entry name" value="Uncharacterized protein, isoform Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF38">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00735">
        <property type="entry name" value="ZM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="8"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="335"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="2108"/>
            <end position="2167"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="2168"/>
            <end position="2223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="549"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="623"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="668"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="939"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UYB9</accession>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V0B6</accession>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002134729.2">
        <property type="nucleotide sequence ID" value="XM_002134693.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V0B6"/>
    <dbReference type="KEGG" id="dpo:6900936"/>
    <dbReference type="InParanoid" id="A0A6I8V0B6"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V0B6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002134729.2"/>
        </source>
    </evidence>
    <sequence length="111" mass="12467" checksum="A74F43C45DF010A4" modified="2020-08-12" version="1">MNRLTNSTFLKMSDALMSTRRTKSKKGNQKDCCQDSNQKKPCCPKQKDSCPKQDCCPKQDCCPKQDCCQDQCKKDSCQNDCCEDSCQKQNCCDQDPCNKKGGKGGKKGWFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V121</accession>
    <name>A0A6I8V121_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA-binding protein with serine-rich domain 1-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6899921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002135289.2">
        <property type="nucleotide sequence ID" value="XM_002135253.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V121"/>
    <dbReference type="KEGG" id="dpo:6899921"/>
    <dbReference type="InParanoid" id="A0A6I8V121"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054054">
        <property type="entry name" value="Ng_1-3-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21827">
        <property type="entry name" value="New_glue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026117287" evidence="2">
        <location>
            <begin position="19"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002135289.2"/>
        </source>
    </evidence>
    <sequence length="134" mass="14323" checksum="347A338642B595D8" modified="2020-08-12" version="1">MRFSFVLLLAVLGCFLLAREGGATGSSTTVASPSSSTTTSSSAASTTAATSTATTTVAPSAATTTVASSTSDTTTASSSSSGSSGSAAARRRRRLRRRRLARQRQRRERQRQRQTQRLRNRIQNQRRVINQLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V273</accession>
    <name>A0A6I8V273_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC6896925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137110.2">
        <property type="nucleotide sequence ID" value="XM_002137074.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V273"/>
    <dbReference type="KEGG" id="dpo:6896925"/>
    <dbReference type="InParanoid" id="A0A6I8V273"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_002137110.2"/>
        </source>
    </evidence>
    <sequence length="375" mass="42821" checksum="BF7F19EC02723227" modified="2020-08-12" version="1">MEKYEKTKKVQDVAGNPNPRQMFDEIVEDIAKSLSQILLICGIKTTKSCQAKSIIRLAFKHHEKLRTKCSPKEAAEPRLDRDDMLQALSIRCGMQSVEAMLSYSIIKCAFKAFFNGKPPVSLSSPIVDAKALHEQQRAWMNASNETSKLFENYQSAFFWAHKSYKEQIDIIYRALYNRMSVLCNPNEGPLCSKPVVPNKLTLKDGCHLRRASILCGLQVPPQLNTGTLVNVQLPRPLRNAEVKVPRCDHCNGRFLICECNIDQLTAEQCQDSYNVKWYRLEDEQPQPKPQWPLCEEDMVPSVDCKHDWSRESMNVCPNVFDGGSSNYQSCTDLSDKMVDKLEDYLIKLWAEDAAEKKNSQTYICGQDHSRNRTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V2C3</accession>
    <name>A0A6I8V2C3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Unconventional myosin-XVIIIa-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6897016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137201.3">
        <property type="nucleotide sequence ID" value="XM_002137165.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V2C3"/>
    <dbReference type="KEGG" id="dpo:6897016"/>
    <dbReference type="InParanoid" id="A0A6I8V2C3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="251"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_002137201.3"/>
        </source>
    </evidence>
    <sequence length="551" mass="63794" checksum="96146B1B5CEA3A94" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V393</accession>
    <name>A0A6I8V393_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC6897659</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137798.4">
        <property type="nucleotide sequence ID" value="XM_002137762.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V393"/>
    <dbReference type="FunCoup" id="A0A6I8V393">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897659"/>
    <dbReference type="InParanoid" id="A0A6I8V393"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247715">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002137798.4"/>
        </source>
    </evidence>
    <sequence length="485" mass="54113" checksum="715B04028E76B3F1" modified="2020-08-12" version="1">MGRSLLKSRSNQVLKCGEFYLKTNPQLSSLRKYSDGGSLKEALAKKIPQEREKMLGIKCLHGDKKLGDIKVSNVMGGQRGLLLLMCDTSYVDPNKGIFYRGIALKDVCAKLPRVQKDSKEGTPEGAFYLLTAGKMPNKKQAKDVTTEWQKRGKLPDYCFKLLDSMDKRIHPMAQFCAASAALNPESKFVEAYTKGAKKPKYWEYIYEDSMNLCAYLPTVAAVIYCNIFKGGKGNRKLESSKDWSDNFCNMMGYKDKGFVDLMRLYLILHADHESGNVSAHTTQLVGSALSDPYLSFSAGMCGLAGPLHGLANQEVLVWLNQLIEAIGCEPTDEEILKFINDTLKSGRVVPGYGHAVLRQTDPRFSLQNAFAMKNCKDDPSVKLVTRLWKLIPKVLKELGKVANPYPNVDAHSGVLLQYYGMTEMKYYTVLFGVSRALGVLPQLIWARALGAPIERPKSFTTDFLCKFLTKSKDKGKKSKKGPKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3B0</accession>
    <name>A0A6I8V3B0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6896964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002137149.2">
        <property type="nucleotide sequence ID" value="XM_002137113.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3B0"/>
    <dbReference type="KEGG" id="dpo:6896964"/>
    <dbReference type="InParanoid" id="A0A6I8V3B0"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026222305" evidence="1">
        <location>
            <begin position="27"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002137149.2"/>
        </source>
    </evidence>
    <sequence length="115" mass="12636" checksum="B0B7473416DD5FF2" modified="2020-08-12" version="1">MNATGLLLLMGLALCLELFLLGPTSCAPLSEELEAPSPIKEIGGLAAFAYSRKKLPKKQGQKNLIMPLSKNMTRRQRMEYADNCEPLGMVCKFAEQCCTRVCMVATKRCSYVGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V3S9</accession>
    <name>A0A6I8V3S9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6898099</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002138181.1">
        <property type="nucleotide sequence ID" value="XM_002138145.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V3S9"/>
    <dbReference type="SMR" id="A0A6I8V3S9"/>
    <dbReference type="GeneID" id="6898099"/>
    <dbReference type="KEGG" id="dpo:6898099"/>
    <dbReference type="InParanoid" id="A0A6I8V3S9"/>
    <dbReference type="OMA" id="HDYSKIS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246013">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V3S9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002138181.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="25215" checksum="FF4F50753307CA24" modified="2020-08-12" version="1">MASSLKKMLISMNFVHATVSLLVIVCGISFFIDEVSTLEKVAGSLTIAQGAVGLILSIFCRGAAKNESLNCLRNYVAIAMLQIFSQLLLLYEAANSSVRIGRIFDDLWEADRRGLALQRRLGFYEKFLKCCGVNGADDYSKIHLHPPKSCCIDEESEFDSPLHPYGCKTQFSDYVFSKLLLLKLACWVLIVVGTNGALCGWRLYRIVKRQIQRDNDCLDEDSKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V489</accession>
    <name>A0A6I8V489_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Luciferin 4-monooxygenase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC6897630</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002137773.2">
        <property type="nucleotide sequence ID" value="XM_002137737.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V489"/>
    <dbReference type="KEGG" id="dpo:6897630"/>
    <dbReference type="InParanoid" id="A0A6I8V489"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000025">
        <property type="entry name" value="luciferin 4-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF353">
        <property type="entry name" value="GH16244P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="37"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="444"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_002137773.2"/>
        </source>
    </evidence>
    <sequence length="538" mass="60465" checksum="1E720744F6540EEC" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4G3</accession>
    <name>A0A6I8V4G3_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Cadherin-87A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Cad87A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="18">MV-25-SWS-2005</strain>
            <tissue evidence="18">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002137915.2">
        <property type="nucleotide sequence ID" value="XM_002137879.4"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A6I8V4G3">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6897810"/>
    <dbReference type="InParanoid" id="A0A6I8V4G3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="13"/>
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    <dbReference type="FunFam" id="2.60.40.60:FF:000443">
        <property type="entry name" value="AGAP003579-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000266">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000306">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000394">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000098">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000378">
        <property type="entry name" value="Cadherin-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cadherin-related family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000168">
        <property type="entry name" value="Cadherin-related family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000445">
        <property type="entry name" value="GG17179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000118">
        <property type="entry name" value="protocadherin Fat 4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="15"/>
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        <property type="entry name" value="Cadherin-like_dom"/>
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        <property type="entry name" value="Cadherin-like_sf"/>
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    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
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    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
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        <property type="entry name" value="CADHERIN-3"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="CADHERIN"/>
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        <property type="entry name" value="CA"/>
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        <property type="entry name" value="Cadherin-like"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
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    </dbReference>
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    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026324812" evidence="15">
        <location>
            <begin position="20"/>
            <end position="1984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1786"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="28"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="133"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="245"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="359"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="473"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="682"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="787"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="891"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="1017"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="1116"/>
            <end position="1223"/>
        </location>
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    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="1224"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="1343"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="1446"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="1566"/>
            <end position="1689"/>
        </location>
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        <location>
            <begin position="534"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1902"/>
            <end position="1927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="572"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="601"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1902"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1915"/>
            <end position="1927"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_002137915.2"/>
        </source>
    </evidence>
    <sequence length="1984" mass="218959" checksum="4D9E51B5A26E29E0" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4L3</accession>
    <name>A0A6I8V4L3_DROPS</name>
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        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC6898680</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="17">MV-25-SWS-2005</strain>
            <tissue evidence="17">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
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        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
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        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026079669" description="procollagen-proline 4-dioxygenase" evidence="14">
        <location>
            <begin position="20"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="15">
        <location>
            <begin position="401"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_002138685.4"/>
        </source>
    </evidence>
    <sequence length="519" mass="59651" checksum="B2CC70A08FED4AF2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V4Z7</accession>
    <name>A0A6I8V4Z7_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">carboxylesterase</fullName>
            <ecNumber evidence="8">3.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC6899081</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.1" evidence="8"/>
    <dbReference type="RefSeq" id="XP_002138998.2">
        <property type="nucleotide sequence ID" value="XM_002138962.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V4Z7"/>
    <dbReference type="ESTHER" id="drops-b5dz85">
        <property type="family name" value="Juvenile_hormone_esterase"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6899081"/>
    <dbReference type="InParanoid" id="A0A6I8V4Z7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43142:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026267501" description="carboxylesterase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="10">
        <location>
            <begin position="31"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_002138998.2"/>
        </source>
    </evidence>
    <sequence length="564" mass="62509" checksum="D097A32617B64880" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V585</accession>
    <name>A0A6I8V585_DROPS</name>
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein Ir94d</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Ir94d</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_003736263.2">
        <property type="nucleotide sequence ID" value="XM_003736215.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V585"/>
    <dbReference type="FunCoup" id="A0A6I8V585">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:13036366"/>
    <dbReference type="InParanoid" id="A0A6I8V585"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF41">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_003736263.2"/>
        </source>
    </evidence>
    <sequence length="589" mass="68601" checksum="54F79F61FF081F46" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V5C5</accession>
    <name>A0A6I8V5C5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Modifier of mdg4-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC13036455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_003736334.2">
        <property type="nucleotide sequence ID" value="XM_003736286.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V5C5"/>
    <dbReference type="FunCoup" id="A0A6I8V5C5">
        <property type="interactions" value="434"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A6I8V5C5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V5C5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18315">
        <property type="entry name" value="BTB_POZ_BAB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000036">
        <property type="entry name" value="Mod(Mdg4), isoform H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051095">
        <property type="entry name" value="Dros_DevTransReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110">
        <property type="entry name" value="BTB DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23110:SF92">
        <property type="entry name" value="MODIFIER OF MDG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="115"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_003736334.2"/>
        </source>
    </evidence>
    <sequence length="600" mass="65190" checksum="47C768FCAEFF962F" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V6G3</accession>
    <name>A0A6I8V6G3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vang-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Vang</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the Vang family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_004444241.2">
        <property type="nucleotide sequence ID" value="XM_004444184.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8V6G3"/>
    <dbReference type="FunCoup" id="A0A6I8V6G3">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:6898532"/>
    <dbReference type="InParanoid" id="A0A6I8V6G3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0246231">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009539">
        <property type="entry name" value="VANGL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20886">
        <property type="entry name" value="VANG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06638">
        <property type="entry name" value="Strabismus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007991">
        <property type="entry name" value="Strabismus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_004444241.2"/>
        </source>
    </evidence>
    <sequence length="590" mass="66712" checksum="FFCB3414BC9307A1" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8V7S3</accession>
    <name>A0A6I8V7S3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protocadherin-16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Protein dachsous homolog 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26 27 28" type="primary">ft</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27 28" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="26 27 28">MV-25-SWS-2005</strain>
            <tissue evidence="26 27 28">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="15">Heterophilic interaction with FAT4; this interaction affects their respective protein levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001357109.4">
        <property type="nucleotide sequence ID" value="XM_001357073.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015035642.2">
        <property type="nucleotide sequence ID" value="XM_015180156.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236389.1">
        <property type="nucleotide sequence ID" value="XM_033380498.1"/>
    </dbReference>
    <dbReference type="IntAct" id="A0A6I8V7S3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817792"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8V7S3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016327">
        <property type="term" value="C:apicolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001737">
        <property type="term" value="P:establishment of imaginal disc-derived wing hair orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042067">
        <property type="term" value="P:establishment of ommatidial planar polarity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035332">
        <property type="term" value="P:positive regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090251">
        <property type="term" value="P:protein localization involved in establishment of planar polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035159">
        <property type="term" value="P:regulation of tube length, open tracheal system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000029">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000592">
        <property type="entry name" value="Cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000594">
        <property type="entry name" value="cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000768">
        <property type="entry name" value="Cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000207">
        <property type="entry name" value="Cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000263">
        <property type="entry name" value="Cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000286">
        <property type="entry name" value="Cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000321">
        <property type="entry name" value="Cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000329">
        <property type="entry name" value="Cadherin-related tumor suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000116">
        <property type="entry name" value="Dachsous cadherin-related 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000015">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000032">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000033">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000037">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000080">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 3"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000039">
        <property type="entry name" value="FAT atypical cadherin 3"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000101">
        <property type="entry name" value="FAT atypical cadherin 4"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 4"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000108">
        <property type="entry name" value="FAT atypical cadherin 4"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000143">
        <property type="entry name" value="FAT atypical cadherin 4"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000144">
        <property type="entry name" value="FAT atypical cadherin 4"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000154">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000092">
        <property type="entry name" value="Protocadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000035">
        <property type="entry name" value="Protocadherin Fat 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000081">
        <property type="entry name" value="protocadherin Fat 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000118">
        <property type="entry name" value="protocadherin Fat 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000134">
        <property type="entry name" value="protocadherin Fat 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000340">
        <property type="entry name" value="Protocadherin Fat 4"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF51">
        <property type="entry name" value="PROTOCADHERIN-LIKE WING POLARITY PROTEIN STAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25374">
        <property type="entry name" value="Cadherin_FAT4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
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    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_015037378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_015037379.1"/>
        </source>
    </evidence>
    <sequence length="248" mass="28749" checksum="794A33755EE8ECE3" modified="2020-08-12" version="1">MSNSNHYNNYQQQQPHHSSSNGDEYQHQQMVHQPHQQRYSNGHGMKSVAVPDMCLFCFEVLDCELNNIDGPGVPIFSNDAYPLFVTWKIGRDKRLRGCIGTFSAMELHNGLREYALTSAFKDSRFAPISRDEFSRLTVSVSILQNFEEAQGHLDWQLGVHGIRIEFLTERGCKRTATYLPQVATEQGWDQLQTIDSLLRKGGYRAAITQEMRKSIKLTRYRSQEIQMHYKEYREHLERRGGQFGKVQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VCV0</accession>
    <name>A0A6I8VCV0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AN1-type zinc finger protein 6 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">drn</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015038639.1">
        <property type="nucleotide sequence ID" value="XM_015183153.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VCV0"/>
    <dbReference type="GeneID" id="4800605"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077365">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VCV0">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF149">
        <property type="entry name" value="AN1-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="6">
        <location>
            <begin position="113"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_015038639.1"/>
        </source>
    </evidence>
    <sequence length="178" mass="19615" checksum="CDF365D354B309B3" modified="2020-08-12" version="1">MDKSEGGEQPERHNQKRRLDLEQDTADIVDSSPKRKPTPGDTKPHDTKEFPLNVPVAVPSKRAKIIVEAAAAAAAKVNTEAITSATGPNTSTQAAASANEEDDKDKEDDKDAKKKKNRCGECRKKVGLTGFQCRCGGLYCAVHRYSDKHNCTFDYREHGAQEIRRNNPVVVGEKIQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VIB4</accession>
    <name>A0A6I8VIB4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Trypsin I-P1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC6901466</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_015041725.2">
        <property type="nucleotide sequence ID" value="XM_015186239.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VIB4"/>
    <dbReference type="InParanoid" id="A0A6I8VIB4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000111">
        <property type="entry name" value="Blast:Serine protease nudel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF65">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026099154" evidence="7">
        <location>
            <begin position="24"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="56"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_015041725.2"/>
        </source>
    </evidence>
    <sequence length="337" mass="36396" checksum="D30FAB3165E057A9" modified="2020-08-12" version="1">MDIPWQLQVLLQMLLSWTLVANGHLEFPFLSLSTGHTSSPHNEPSDAGKFVILPKVVGGYSITIEQVPFQVSVRRRSMHERSYGLGLICGGALISQRVACSAAHCFAVNNSYNPVRFYGPKLFVVVAGSTQIDQADRNTKEYLVQQIIGHSSYNATTLENDIALIFLNGYVSWSSKTVQAIPLATKAQREGTTCLINGWAAKISPKVGKSGSLQQAPVPILNPSLCRAIYLLPMSQLCAGFMQGGIDACQGDSGGPLICDGQLAGIISWGVGCADPGFPGVYTNVSYFANWIKSMNASLDYSKYLVMDSADGRRLGASPQRTWWLALAFLLIALRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VL08</accession>
    <name>A0A6I8VL08_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcriptional repressor scratch 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Scratch homolog 1 zinc finger protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">LOC4812011</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17">MV-25-SWS-2005</strain>
            <tissue evidence="16 17">Whole body</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_015043556.2">
        <property type="nucleotide sequence ID" value="XM_015188070.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_015043557.2">
        <property type="nucleotide sequence ID" value="XM_015188071.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VL08"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VL08">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055059">
        <property type="term" value="P:asymmetric neuroblast division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060562">
        <property type="term" value="P:epithelial tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000177">
        <property type="term" value="P:regulation of neural precursor cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000043">
        <property type="entry name" value="Scratch family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000169">
        <property type="entry name" value="transcriptional repressor scratch 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001484">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000207">
        <property type="entry name" value="zinc finger protein SNAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF38">
        <property type="entry name" value="PROTEIN SNAIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="475"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="501"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
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        <location>
            <begin position="557"/>
            <end position="575"/>
        </location>
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        <location>
            <begin position="24"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="311"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="377"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="598"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="311"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="612"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064979"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00071743"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083685"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_015043556.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_015043557.2"/>
        </source>
    </evidence>
    <sequence length="633" mass="67491" checksum="43738E024FCC4B7A" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VP17</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="3" type="primary">LOC117183297</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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        </source>
    </reference>
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        <property type="component" value="Chromosome 2"/>
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        <property type="entry name" value="UPF0184"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033232613.1"/>
        </source>
    </evidence>
    <sequence length="97" mass="11342" checksum="0FA3C5B5157DA85A" modified="2020-08-12" version="1">MDRSEEDEQPTEESQRYRSRQLNASVDALVVLRRNTERIMDSVIMNLDALNMALDHLEERTDRVLAQLRQYMQSMEDLDRSNTARWHGGYGDPDATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VPZ0</accession>
    <name>A0A6I8VPZ0_DROPS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="primary">LOC6899372</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
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            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">MV2-25</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="2">MV-25-SWS-2005</strain>
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    </dbReference>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_033233116.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="20730" checksum="19C4592553E589D3" modified="2020-08-12" version="1">MDNTSKVDNSEPSFQDAVVFKFTNFVCESYNQSWFVFHNYRLKAVSRNKVLLNVNGTILHPVKNASIRGRIFKKANGYKPWLFDIYFDACQYFLKRNQPVVNIVYRLFKEFTSLNHSCPYVGPQFVKDLYLRHELLGIPLPTGDYLLAIQWYFDKKHQFDTNVSFTFVEDLMKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VQB4</accession>
    <name>A0A6I8VQB4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Odorant receptor 98a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC117183391</name>
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    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
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            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033233068.1">
        <property type="nucleotide sequence ID" value="XM_033377177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VQB4"/>
    <dbReference type="KEGG" id="dpo:117183391"/>
    <dbReference type="InParanoid" id="A0A6I8VQB4"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004117">
        <property type="entry name" value="7tm6_olfct_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21137:SF35">
        <property type="entry name" value="ODORANT RECEPTOR 19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02949">
        <property type="entry name" value="7tm_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033233068.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13393" checksum="94DF2286AB073A86" modified="2020-08-12" version="1">MLVGLVLGISLINLYLFADTWAKLAIAAYIVVQIVQTFPFCYTCDLIREDCESLAVAIFHSNWKGSSRRYRSSLIYFLQNAQRTISFSAGSVFPICLNTNIRVAKLAFSVVTFVKQVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VXB2</accession>
    <name>A0A6I8VXB2_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trypsin epsilon-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC117184000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033235730.1">
        <property type="nucleotide sequence ID" value="XM_033379839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VXB2"/>
    <dbReference type="KEGG" id="dpo:117184000"/>
    <dbReference type="InParanoid" id="A0A6I8VXB2"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VXB2">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026213254" evidence="1">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033235730.1"/>
        </source>
    </evidence>
    <sequence length="61" mass="6449" checksum="E35BFA4510B16665" modified="2020-08-12" version="1">MLFKVSLLFATATLLSAGRVPRIIGGQDTPIEEDPWQVSLVVGGDHACGGSIYSKDFVITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VYM7</accession>
    <name>A0A6I8VYM7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Thioredoxin-related transmembrane protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC117184111</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16">MV-25-SWS-2005</strain>
            <tissue evidence="16">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033236206.1">
        <property type="nucleotide sequence ID" value="XM_033380315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VYM7"/>
    <dbReference type="KEGG" id="dpo:117184111"/>
    <dbReference type="InParanoid" id="A0A6I8VYM7"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VYM7">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052454">
        <property type="entry name" value="TMX_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46107">
        <property type="entry name" value="DUMPY: SHORTER THAN WILD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46107:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026170916" evidence="13">
        <location>
            <begin position="24"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="32"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_033236206.1"/>
        </source>
    </evidence>
    <sequence length="113" mass="12914" checksum="A3A6CC28D659D817" modified="2020-08-12" version="1">MQCNSQLVAASCVIALLMSTAQAGLQPGGKIIELDEDNWHLMLKGEWMIEFFAPWCPACKNLGPTWERFARVAKDVKVEVAKMDVTSSKKSRKTRRRTMKPMMMVSRRRPTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZ69</accession>
    <name>A0A6I8VZ69_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chymotrypsin inhibitor SCI-III-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC26534025</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the venom Kunitz-type family. 03 (sub-Kunitz) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033235814.1">
        <property type="nucleotide sequence ID" value="XM_033379923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZ69"/>
    <dbReference type="KEGG" id="dpo:26534025"/>
    <dbReference type="InParanoid" id="A0A6I8VZ69"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VZ69">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051388">
        <property type="entry name" value="Serpin_venom_toxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46751">
        <property type="entry name" value="EPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46751:SF1">
        <property type="entry name" value="WAP FOUR-DISULFIDE CORE DOMAIN PROTEIN 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026131053" evidence="3">
        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="4">
        <location>
            <begin position="25"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033235814.1"/>
        </source>
    </evidence>
    <sequence length="78" mass="8658" checksum="2D8CDFC88C3F0CA0" modified="2020-08-12" version="1">MKLISIVGLIFALSGVAIGLKNRICGQKSAHFGYCSGYTKGFTYYPETNECKMFVYGRCAGNKNRFKDKAVCEAKCKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZ82</accession>
    <name>A0A6I8VZ82_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mucin-5AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Muc96D</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="5">MV-25-SWS-2005</strain>
            <tissue evidence="5">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236372.1">
        <property type="nucleotide sequence ID" value="XM_033380481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZ82"/>
    <dbReference type="KEGG" id="dpo:26532175"/>
    <dbReference type="InParanoid" id="A0A6I8VZ82"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.140.10:FF:000019">
        <property type="entry name" value="Mucin 26B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026127757" evidence="2">
        <location>
            <begin position="22"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="3">
        <location>
            <begin position="811"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_033236372.1"/>
        </source>
    </evidence>
    <sequence length="868" mass="87973" checksum="CC980D53E9FA66E2" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8VZA1</accession>
    <name>A0A6I8VZA1_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Phenoloxidase-activating factor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC6903044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033236430.1">
        <property type="nucleotide sequence ID" value="XM_033380539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8VZA1"/>
    <dbReference type="KEGG" id="dpo:6903044"/>
    <dbReference type="InParanoid" id="A0A6I8VZA1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8VZA1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051333">
        <property type="entry name" value="CLIP_Serine_Protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24260:SF145">
        <property type="entry name" value="FI17609P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026227836" evidence="1">
        <location>
            <begin position="21"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_033236430.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="33057" checksum="9BB298E411DFE032" modified="2020-08-12" version="1">MFGGAFIGLMGLLVLSTIQAQQQQPCGRLRQHQLYNRREITDPDEYPWLGRIGYSSNESGADPKEYRYLCLAVLLRERHAILPAHCIGEDSSMEPTTILFGDWRSNQATHERDCLTADSCAPPPESYTILELAIHPQYDRIRHDHDIALAKLDRNVRFGDFVQPICLPPDHEVEGNHIGQRLEMVGFLRTTGTEGSWRWKRQVHTLGSKYCSTHPGFAGFNLSSNHICGLISSTEPFMSGSPMMGVNVVDGNPDSFYLIATATFGVSRKREIVPDGFMRIYPFREWIVRNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W0T4</accession>
    <accession>Q29KW1</accession>
    <name>A0A6I8W0T4_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Synaptotagmin 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17 18" type="primary">Syt1</name>
        <name evidence="16" type="synonym">LOC4816515</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17 18" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="16 17 18">MV-25-SWS-2005</strain>
            <strain evidence="16">MV2-25</strain>
            <tissue evidence="16 17 18">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356004.1">
        <property type="nucleotide sequence ID" value="XM_001355968.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236359.1">
        <property type="nucleotide sequence ID" value="XM_033380468.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_033236360.1">
        <property type="nucleotide sequence ID" value="XM_033380469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W0T4"/>
    <dbReference type="SMR" id="A0A6I8W0T4"/>
    <dbReference type="GeneID" id="4816515"/>
    <dbReference type="KEGG" id="dpo:4816515"/>
    <dbReference type="OMA" id="AXKEEEL"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076240">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W0T4">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08402">
        <property type="entry name" value="C2B_Synaptotagmin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000007">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000016">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF227">
        <property type="entry name" value="SYNAPTOTAGMIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="191"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="324"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_001356004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033236359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_033236360.1"/>
        </source>
    </evidence>
    <sequence length="473" mass="53096" checksum="5A39EBC2D872DC42" modified="2020-08-12" version="1">MPPNAKSEADAKPDAEPAPESATELDSVDQKLEETHHSRFRELEKQEQESAAEEAAAAAAESQRIAQVETTTRSATTEAQESTTTSLPVIKKIEHAGEVVTEAIAERTGLPTWGVVAIIILVFLVVFGIIFFCVRRFLKKRRTKDGKGKKGVDMKSVQLLGSAYKEKVQPDMEELAENAEEDEEDKQSEQKLGRLNFKLEYDFNSNSLAVTVIQAEELPALDMGGTSDPYVKVYLLPDKKKKFETKVHRKTLSPVFNETFTFKSLPYADAMNKTLVFAIFDFDRFSKHDQIGEVKVPLCTIDLAQTIEEWRDLVSVEGEGGQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIAIMQNGKRLKKKKTSIKKCTLNPYYNESFSFEVPFEQIQKICLVVTVVDYDRIGTSEPIGRCILGCMGTGTELRHWSDMLASPRRPIAQWHTLKDPEETDEILKNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W240</accession>
    <name>A0A6I8W240_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6899912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033237397.1">
        <property type="nucleotide sequence ID" value="XM_033381506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W240"/>
    <dbReference type="KEGG" id="dpo:6899912"/>
    <dbReference type="InParanoid" id="A0A6I8W240"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.220.10">
        <property type="entry name" value="Ganglioside GM2 activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036846">
        <property type="entry name" value="GM2-AP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033237397.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="27264" checksum="54C4C91E87245820" modified="2020-08-12" version="1">MCLFSDRGQRTDDTGSGVIGSRFLGLVKSNGSSDDVYVTACGVSVSVSVLRRGGEYFPGIFHPQRVSHSLSRQDRNIRFIAGESRFNREYFDNFSFSIRNDQIFLEMNLRRPLPKGWRARLDFQLRVGNSKSFQSLFSTSVDVCHIVNAAKMNLFKKWYKNMLKYGNFLRQCPLKAGHYYLRGWQFGEGLVPPFITSGSYRLQTYNFYGKYKGKDEDFIMSCTADAIIHQPSSDLASNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W496</accession>
    <name>A0A6I8W496_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein Ir7f</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ir7f</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_033238157.1">
        <property type="nucleotide sequence ID" value="XM_033382266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W496"/>
    <dbReference type="FunCoup" id="A0A6I8W496">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:117184476"/>
    <dbReference type="InParanoid" id="A0A6I8W496"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W496">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052192">
        <property type="entry name" value="Insect_Ionotropic_Sensory_Rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056198">
        <property type="entry name" value="LBD_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643">
        <property type="entry name" value="IONOTROPIC RECEPTOR 20A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42643:SF30">
        <property type="entry name" value="IONOTROPIC RECEPTOR 40A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24061">
        <property type="entry name" value="LBD_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ionotropic receptor ligand binding" evidence="9">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_033238157.1"/>
        </source>
    </evidence>
    <sequence length="466" mass="53649" checksum="BCA484E34C3E7C96" modified="2020-08-12" version="1">MELGLILEHCLAHYWLHCNVMIQTADVDVLVYSYYPYTSDHCQAAYPVQVNHFDGQRMVNPTMFPEKLHQLHGCPISVLTWHQPPFVELKWDEHSNSLRASGFEIQLVDHLARLLNFTVDLINLTLIQPQLYRLANGSSEGPMEFLLERRANLSLGYFRKTARRNQLLTTGMSYYSSPLVAVLQRETYLIGSLALLTFPFEWTVWLILLGSLALQIAIRRAGVPVLELLLGQSLARLPRSWLHRLVFAHWLYAVMPLRICYQSLLFHLIRMQLFAALPVSLEQLLAENFQGVCTGNTLRMLHEMPLVAGRTESFSGLATPYDQEVLDSLQQTGGHKVFAIAGMDVTLAYLRSSSRPDSYHVVGQPVNVEYAGLYMPKHSYLYEKVDHAIRRLDDTGFIQAWRRAAFVAHRREEKLPQEHDRRRYISNSQLSGVYKLMTVLYSICGFVFAGELLLQRWRDRRTGRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W5H3</accession>
    <name>A0A6I8W5H3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Folliculin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC4813717</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the folliculin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033238537.1">
        <property type="nucleotide sequence ID" value="XM_033382646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8W5H3"/>
    <dbReference type="SMR" id="A0A6I8W5H3"/>
    <dbReference type="FunCoup" id="A0A6I8W5H3">
        <property type="interactions" value="1347"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4813717"/>
    <dbReference type="InParanoid" id="A0A6I8W5H3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8W5H3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1730">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044886">
        <property type="entry name" value="FLCN_DENN_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032035">
        <property type="entry name" value="Folliculin_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16692">
        <property type="entry name" value="Folliculin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="17">
        <location>
            <begin position="52"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="173"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_033238537.1"/>
        </source>
    </evidence>
    <sequence length="468" mass="53155" checksum="BA8C85431AF97B24" modified="2020-08-12" version="1">MNAVIALCHFCEAHGPCAIFCTQTLRDTKIEDLPLEQHQQQQSQKTCSACNYSMGRNNSGIYSKDTESGATFVSTRVAVLTEVATMVKQAAVLSLSNGIDSNKDGEFVFFGDSTRGHILSHKFRVSDLQARGYFQLYSIIVLMKDKYFLLNTKPFLAEHLKKVSSELQAGANKTKETEERRQSERQRRLSGAPFLMHSSRSLLELTGEENIFAQLHSHFSWLLLAGSRFLTEHVTFGNLPWLPHQSSGRPPAQRLTYNSSTLPMIQKYEESIDDPDQEEFFSLRHIKNVVRKEEFATVCYCALTGVKIIVRGAPERTFRFMVCLKKLLPEPMHNLMRIDAQHQHSISSEYKIISVSNDIAVPIASASVFRIDFLDKHVNGHEVSVKWPGELPRKLPDLMVKLLKAVEEKIFTELVLNKQTKVLIEEWKNKATCLNHAKSTSVQAKLKKVLGVQPQDQPLINYWSTHLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W6M4</accession>
    <name>A0A6I8W6M4_DROPS</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein Hers isoform X1</fullName>
        </submittedName>
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        <name evidence="4" type="primary">Hers</name>
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    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
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        <property type="nucleotide sequence ID" value="XM_033383107.1"/>
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        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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        <property type="entry name" value="BAH_dom"/>
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        <property type="entry name" value="BAH_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
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    <dbReference type="PANTHER" id="PTHR46576">
        <property type="entry name" value="BROMO ADJACENT HOMOLOGY DOMAIN-CONTAINING 1 PROTEIN"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8W7X7</accession>
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    <sequence length="620" mass="71990" checksum="B1E40D7D96FEC0BD" modified="2020-08-12" version="1"> </sequence>
</entry>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="377" mass="38176" checksum="0FDB61511DC7272E" modified="2020-08-12" version="1">MQLFAVLSLALVALASADVSHLSNTYLPPRSGAASTSYESYQSAPAESASFASESYSAPAEAASSTDDSESYVAPAATYNSESYAAPAESNAETAAEESYAQPAAVAAASYSAPVVTKTYSAPAVTSYTNTYTAPAVTSYTAPAVVKTYSAPAVTSYTAPAVVRSYSAPAVTSYTKTYSAPAVTSYTAPAVVKTYSAPAVTSYTAPAVVKSYSAPAVTSYTKTYSAPAVTSYTAPAVVKTYSAPAVTSYTAPAVVKTYSAPAVSSYIAPAVVKSYSAPAVTSYTKTYSAPAVTSYTAPAVVKTYSAPAVTSYTAPAVVKTYSAPAVSSYTAPAVSTYSAPAVSTYSAPAVSTYRAPAVVSGYSGIDTTYGTNGGYVY</sequence>
</entry>
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        <location>
            <begin position="1"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033240210.1"/>
        </source>
    </evidence>
    <sequence length="903" mass="100192" checksum="9DD8EF6E84C19081" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WB78</accession>
    <name>A0A6I8WB78_DROPS</name>
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        <submittedName>
            <fullName evidence="3 4">Protein piccolo isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">mxc</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3 4">MV-25-SWS-2005</strain>
            <tissue evidence="3 4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_015041477.2">
        <property type="nucleotide sequence ID" value="XM_015185991.2"/>
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        <property type="nucleotide sequence ID" value="XM_033384769.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="LisH"/>
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        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A CAF-1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        </location>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WC39</accession>
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        <recommendedName>
            <fullName evidence="3">Reverse transcriptase/retrotransposon-derived protein RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">LOC117185138</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Ecdysozoa</taxon>
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            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
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            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
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            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
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        <source>
            <dbReference type="RefSeq" id="XP_033240976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18888" checksum="13A7A64D93B01674" modified="2020-08-12" version="1">MQEIWLQDLGWDDELPSEMRQRWQSFLRSYSALDQIHIPRWVGSRPAVKVEHHVFCDASERAYGAAIYVRIEVDRLVEVQLLTAKTRVAPVKTVSLPRLELCGAVLLSEMAAAILPNMSTASTSCYRWTDSTIVLSWLAKPACHWTTFVANRVTRISQATDNAVYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WCL5</accession>
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            <fullName evidence="13">NADH-cytochrome b5 reductase</fullName>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="17">MV-25-SWS-2005</strain>
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        </source>
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        <reaction evidence="13">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
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            <name>FAD</name>
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        <text evidence="2 13">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
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    </dbReference>
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        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD-bd_FR_type"/>
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        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
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        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF185">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0274">FAD</keyword>
    <keyword evidence="4 12 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0520">NAD</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
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        <location>
            <begin position="61"/>
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    </feature>
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            <position position="113"/>
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        <ligand>
            <name>FAD</name>
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        </ligand>
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        </ligand>
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            <name>FAD</name>
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            <position position="206"/>
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            <name>FAD</name>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_033241151.1"/>
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    </evidence>
    <sequence length="323" mass="36110" checksum="F50E1FEBE7E579E7" modified="2020-08-12" version="1">MRVEISSLFSLIASGFVPIAVGVVAVVAGALIVHYFLNKKSTKPRREPNRTARLRTLVDPNDKYLLPLVEKEVLSVDTRRFRFGLPSKQHVLGLPVGQHIHLIATIDNELIIRPYTPISSDDDVGYVDLVVKVYFKDTHPKFPGGGKMTQHLEKMELGDKISFRGPSGRLQYLGNGTFSIKKLRKDPPKHVTAKRVNMIAGGTGITPMLQLVREVLKRNDKDKTEMALLFANQSEKDILLRGELDELAQKHPGQFKVWYTVDKAAEGWAYSEGFINEDMIAGHLLPASDDTIVLLCGPPPMINFACNPALDKLNFHPDTRFAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8WEG1</accession>
    <name>A0A6I8WEG1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription elongation regulator 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC6897557</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="6">MV-25-SWS-2005</strain>
            <tissue evidence="6">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033241816.1">
        <property type="nucleotide sequence ID" value="XM_033385925.1"/>
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        <property type="component" value="Chromosome 2"/>
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    <dbReference type="Bgee" id="FBgn0248732">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
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        <property type="expression patterns" value="baseline"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000114">
        <property type="entry name" value="Transcription elongation regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000005">
        <property type="entry name" value="Transcription elongation regulator 1 (CA150)"/>
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    </dbReference>
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        <property type="entry name" value="Transcription elongation regulator 1 (CA150)"/>
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    </dbReference>
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        <property type="entry name" value="WW_TCRG1_3rd"/>
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        <property type="entry name" value="WW_TCERG1"/>
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        <property type="entry name" value="FF"/>
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        <property type="entry name" value="WW"/>
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        <property type="match status" value="5"/>
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        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="WW_DOMAIN_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="3">
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            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="3">
        <location>
            <begin position="455"/>
            <end position="488"/>
        </location>
    </feature>
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        <location>
            <begin position="605"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="672"/>
            <end position="727"/>
        </location>
    </feature>
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        <location>
            <begin position="802"/>
            <end position="856"/>
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    </feature>
    <feature type="domain" description="FF" evidence="4">
        <location>
            <begin position="1066"/>
            <end position="1121"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="122"/>
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    </feature>
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        <location>
            <begin position="163"/>
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            <begin position="412"/>
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            <begin position="861"/>
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            <begin position="81"/>
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            <begin position="198"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51676"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_033241816.1"/>
        </source>
    </evidence>
    <sequence length="1208" mass="139060" checksum="AF83AC1D8CB398C8" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2025-06-18" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0ABD8GS68</accession>
    <name>A0ABD8GS68_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytoplasmic tRNA 2-thiolation protein 1</fullName>
            <ecNumber evidence="6">2.7.7.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytoplasmic tRNA adenylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ctu1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2024-06" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">MV2-25</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_001361116.2">
        <property type="nucleotide sequence ID" value="XM_001361079.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0ABD8GS68"/>
    <dbReference type="SMR" id="A0ABD8GS68"/>
    <dbReference type="GeneID" id="4804577"/>
    <dbReference type="KEGG" id="dpo:4804577"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000054">
        <property type="entry name" value="Cytoplasmic tRNA 2-thiolation protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03053">
        <property type="entry name" value="CTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032442">
        <property type="entry name" value="CTU1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16503">
        <property type="entry name" value="zn-ribbon_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="8">
        <location>
            <begin position="52"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Cytoplasmic tRNA 2-thiolation protein 1 C-terminal" evidence="9">
        <location>
            <begin position="281"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="56"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001361116.2"/>
        </source>
    </evidence>
    <sequence length="343" mass="38460" checksum="6F4C6D608B6883DC" modified="2025-06-18" version="1">MPINCKAKCGKAAALKRPKTGDALCKECFFAAFEAEIHHTITSSKLFRRGEKVAVAASGGKDSTVLAHVMKLLNERHDYGLELVLLSIDEGITGYRDDSLETVKQNRDDYKMPLKILSYEELYGWTMDRIVSQIGRSNNCTFCGVFRRQALDRGAKLLCVDSIATGHNADDIAETVLMNVLRGDTARLRRCTDIRTGGGEDSIPRVKPLKYSYEKEIVMYAHYKKLVYFSTECVFAPNAYRGHARAFLKDLEKVRPSVIMDIIYSGEQLRFKDTAKNPVRGTCNRCGFISSQQPCKACVLLEGLNRGLPKLGIGKKSKGDRMIAKQDQELALRERANLVKNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>B5DIR1</accession>
    <accession>A0A6I8UXK9</accession>
    <name>B5DIR1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_002132801.2">
        <property type="nucleotide sequence ID" value="XM_002132765.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DIR1"/>
    <dbReference type="GeneID" id="6903083"/>
    <dbReference type="KEGG" id="dpo:6903083"/>
    <dbReference type="HOGENOM" id="CLU_1074681_0_0_1"/>
    <dbReference type="InParanoid" id="B5DIR1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0247047">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DIR1">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_002132801.2"/>
        </source>
    </evidence>
    <sequence length="258" mass="29150" checksum="21A74AACDED1DE56" modified="2016-01-20" version="2">MSIPRLAREVLPHVAEEQARPDNALIRIEPRQVGDLQAVAVPPTPPAALMGPLAVDGSAPVLLPILDYVRELLANPFPREPESTDYRLHIASHRQRLLECITTVVGILRNVMSAMARDSPLSAEERRNFQKLRTLYFSAFDIGNSLRRTIDAAGHTVYSWPRHYVVMSPGLNSPDTSFGGGSMLFPWRNPYLFTPHLGAHYFERGMEQREAHMEREFWDEHYYLDEASTGPPDNVMEDQNNGTLEERGDGAPHNNRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>B5DMM9</accession>
    <accession>A0A6I8V006</accession>
    <name>B5DMM9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Laccase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Mco4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002134471.1">
        <property type="nucleotide sequence ID" value="XM_002134435.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DMM9"/>
    <dbReference type="SMR" id="B5DMM9"/>
    <dbReference type="FunCoup" id="B5DMM9">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DMM9"/>
    <dbReference type="GeneID" id="6901803"/>
    <dbReference type="KEGG" id="dpo:6901803"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_8_1_1"/>
    <dbReference type="InParanoid" id="B5DMM9"/>
    <dbReference type="OMA" id="WHSHTEH"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245384">
        <property type="expression patterns" value="Expressed in female reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DMM9">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13858">
        <property type="entry name" value="CuRO_1_tcLCC2_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13884">
        <property type="entry name" value="CuRO_2_tcLCC_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13905">
        <property type="entry name" value="CuRO_3_tcLLC2_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000045">
        <property type="entry name" value="Laccase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF394">
        <property type="entry name" value="FI03373P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="121"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="262"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="491"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_002134471.1"/>
        </source>
    </evidence>
    <sequence length="637" mass="72001" checksum="363E8632C933077D" modified="2008-10-14" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>B5DPG6</accession>
    <accession>A0A6I8V1G6</accession>
    <name>B5DPG6_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDAN1-interacting nuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC6900494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="8">MV-25-SWS-2005</strain>
            <tissue evidence="8">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002134884.1">
        <property type="nucleotide sequence ID" value="XM_002134848.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DPG6"/>
    <dbReference type="FunCoup" id="B5DPG6">
        <property type="interactions" value="1597"/>
    </dbReference>
    <dbReference type="STRING" id="46245.B5DPG6"/>
    <dbReference type="GeneID" id="6900494"/>
    <dbReference type="KEGG" id="dpo:6900494"/>
    <dbReference type="eggNOG" id="ENOG502R9SY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076808_0_1_1"/>
    <dbReference type="InParanoid" id="B5DPG6"/>
    <dbReference type="OMA" id="DTKGHKF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245129">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="B5DPG6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029404">
        <property type="entry name" value="CDIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661:SF1">
        <property type="entry name" value="CDAN1-INTERACTING NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661">
        <property type="entry name" value="SIMILAR TO CDNA SEQUENCE BC052040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14811">
        <property type="entry name" value="TPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_002134884.1"/>
        </source>
    </evidence>
    <sequence length="331" mass="37687" checksum="1FFABC4450F6F583" modified="2008-10-14" version="1">MSAVAVDKKKILTSVEYTRICQFVNGFHGLAIDCEFELRNRYFTDVNPRALTCIVQTELFNMSRGQHWRHDHRAKKLLKTYEEQAHLYNDNALIIRMACAEGANPVAMCRVLLQEKYDLKQRPQVSRLLKYPHLISDPQLAANVQQCMYSDNQEGPITDLRRRIIGEEYELKLKQLAKEAGIHFYDEHDLRRMGYDKTPDIKMILPFLYKGTVVNWIESKANFGDPKSHKWNINQQLQSYCNRFGPGIIIYWFGYQEETPTLPDNNIGITVLMDFPAKEDLVFMQLAECQSTAKKSIAPSPTTADPHSLSAAGDQSTTAIAKGAPNPLSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-10-14" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>B5DVB1</accession>
    <accession>A0A6I8W6A4</accession>
    <name>B5DVB1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear pore complex protein Nup160 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC6903645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_033238827.1">
        <property type="nucleotide sequence ID" value="XM_033382936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="B5DVB1"/>
    <dbReference type="SMR" id="B5DVB1"/>
    <dbReference type="STRING" id="46245.B5DVB1"/>
    <dbReference type="KEGG" id="dpo:6903645"/>
    <dbReference type="eggNOG" id="KOG4521">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1497801_0_0_1"/>
    <dbReference type="InParanoid" id="B5DVB1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0243890">
        <property type="expression patterns" value="Expressed in male reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059141">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11715">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoporin Nup120/160 beta-propeller" evidence="1">
        <location>
            <begin position="44"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_033238827.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="14951" checksum="762F9BFBFD5305F2" modified="2016-01-20" version="2">MTDVGSRDIELIMKHTVVKPDMDLINFNVTLLHIWALWSNVEGPPDGYCLTVQHGIDPREAYCPYIFHPGRFDRTLITKALYMFRRVNMQIDVRQLAMTVLKERVCEAVKDKIQNEFKEFVVSDGNRH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-29" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q292U2</accession>
    <name>PRIC1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Protein prickle</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pk</name>
        <name type="ORF">GA10748</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts in a planar cell polarity (PCP) complex; polarization along the apical/basal axis of epithelial cells. PCP signaling in the wing disk requires the receptor fz and the cytoplasmic proteins dsh and pk. These act in a feedback loop leading to activation of the jnk cascade and subsequent polarized arrangement of hairs and bristles. Dgo and pk compete with one another for dsh binding, thereby modulating fz dsh activity and ensuring tight control over fz PCP signaling. Vang, stan and pk function together to regulate the establishment of tissue polarity in the adult eye.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with dsh; PET and LIM domains interact with dsh DEP domain, in wing cells. Interacts with Vang in photoreceptor cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the prickle / espinas / testin family.</text>
    </comment>
    <dbReference type="EMBL" id="CM000071">
        <property type="protein sequence ID" value="EAL24769.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q292U2"/>
    <dbReference type="SMR" id="Q292U2"/>
    <dbReference type="FunCoup" id="Q292U2">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q292U2"/>
    <dbReference type="eggNOG" id="KOG1704">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008937_0_0_1"/>
    <dbReference type="InParanoid" id="Q292U2"/>
    <dbReference type="OMA" id="GQEYDTV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001736">
        <property type="term" value="P:establishment of planar polarity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007164">
        <property type="term" value="P:establishment of tissue polarity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09415">
        <property type="entry name" value="LIM1_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09418">
        <property type="entry name" value="LIM2_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09420">
        <property type="entry name" value="LIM3_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09827">
        <property type="entry name" value="PET_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000035">
        <property type="entry name" value="prickle-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000005">
        <property type="entry name" value="Testin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033725">
        <property type="entry name" value="LIM1_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033726">
        <property type="entry name" value="LIM2_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033727">
        <property type="entry name" value="LIM3_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010442">
        <property type="entry name" value="PET_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033723">
        <property type="entry name" value="PET_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047120">
        <property type="entry name" value="Pk/Esn/Tes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211:SF20">
        <property type="entry name" value="PROTEIN ESPINAS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06297">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51303">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0217">Developmental protein</keyword>
    <keyword id="KW-0440">LIM domain</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000288835" description="Protein prickle">
        <location>
            <begin position="1"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="domain" description="PET" evidence="4">
        <location>
            <begin position="548"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding 1" evidence="3">
        <location>
            <begin position="655"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding 2" evidence="3">
        <location>
            <begin position="720"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding 3" evidence="3">
        <location>
            <begin position="781"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="130"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="500"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="840"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="933"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1062"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="514"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="861"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="952"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1111"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1130"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1177"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1207"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1228"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1269"/>
            <end position="1284"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="A1Z6W3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="1353" mass="145645" checksum="AA18C037456DBB9A" modified="2009-01-20" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D54</accession>
    <accession>A0A6I8UBZ9</accession>
    <name>Q29D54_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein BRX1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Brix domain-containing protein 2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC4812675</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="10">MV-25-SWS-2005</strain>
            <tissue evidence="10">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BRX1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353059.1">
        <property type="nucleotide sequence ID" value="XM_001353023.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D54"/>
    <dbReference type="SMR" id="Q29D54"/>
    <dbReference type="FunCoup" id="Q29D54">
        <property type="interactions" value="1765"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29D54"/>
    <dbReference type="GeneID" id="4812675"/>
    <dbReference type="KEGG" id="dpo:4812675"/>
    <dbReference type="eggNOG" id="KOG2971">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048373_0_0_1"/>
    <dbReference type="InParanoid" id="Q29D54"/>
    <dbReference type="OMA" id="YRHRHLM"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076804">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000003">
        <property type="entry name" value="Ribosome biogenesis protein BRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026532">
        <property type="entry name" value="BRX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRX1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_001353059.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="41153" checksum="D150821B643AF3D7" modified="2006-04-04" version="1">MGRKFQKKKQVDQLEIVPVDENPPLPPQRKSDDVVPKREKWVNKQRVLVFSARGISHRDRHLMRDIKTLMPHHRAESKMERAKTLSVVNEMCEMKHCNKAMLFEGRRKRDLYMWVSNTVGSEGPSAKFLIENIHTMAELKMTGNCLRGSRPLLSFDSKFDELPHLKLLKELFVQTYSVPHHHPKSQPFVDHVFTFTFLDNRIWFRNFQILSEDGGLSEVGPRYVMNPVKIFDGSFTGKTLWENPDYVSPSKQRQILKKSAKDKYVNRVEQKVQHEATRPVKAYDGIDNDELFDDEDPIATAKILAAIAKKKQQENEQKTPKSALTKKIKEKQLKAIKKVIDQKKAKVTKRVKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q29D98</accession>
    <accession>A0A6I8UBW2</accession>
    <name>Q29D98_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4811891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353015.1">
        <property type="nucleotide sequence ID" value="XM_001352979.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29D98"/>
    <dbReference type="STRING" id="46245.Q29D98"/>
    <dbReference type="GeneID" id="4811891"/>
    <dbReference type="KEGG" id="dpo:4811891"/>
    <dbReference type="eggNOG" id="ENOG502SA0S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_824569_0_0_1"/>
    <dbReference type="InParanoid" id="Q29D98"/>
    <dbReference type="OMA" id="KPQRIEF"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0077632">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29D98">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001353015.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="38211" checksum="C7F48B56C2738B44" modified="2006-04-04" version="1">MSESTDLECANSKSMLIGVHHVGVTAKMQSPLKKVRPRMEMSSESDIFQNVKIKTQYNAFKNDPLTETTVSDHSSPSALYKMRAEPEKLLLGTGKEKPERIEFERYSKRRPRVFVPLRGRARVPQRAKLNKASQAHPMNTGLVNRLMYSLDNMCKCQPQHSHSIIDDLPEPNWNKKEESRHTCIGIYPFEHGCADYLTTTDTHPRINAIVLADRATTYATHFWAELFGLLHIVVAFVVAFILQGYRFVLYSLVNTLIVGLLHLTSDYLLKPVLTTLFNGFLQPPLIFVYNILCSMRDITEPLGDGLGHFLKPLATVCGSIRLIDVNSRLARRLSKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q29DB5</accession>
    <accession>A0A6I8UBU7</accession>
    <name>Q29DB5_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4812092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001352998.2">
        <property type="nucleotide sequence ID" value="XM_001352962.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29DB5"/>
    <dbReference type="GeneID" id="4812092"/>
    <dbReference type="KEGG" id="dpo:4812092"/>
    <dbReference type="eggNOG" id="ENOG502TD5F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124080_0_0_1"/>
    <dbReference type="InParanoid" id="Q29DB5"/>
    <dbReference type="OMA" id="QSGNYPK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0072200">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29DB5">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010512">
        <property type="entry name" value="DUF1091"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898:SF0">
        <property type="entry name" value="DAEDALUS ON 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20898">
        <property type="entry name" value="DAEDALUS ON 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06477">
        <property type="entry name" value="DUF1091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00697">
        <property type="entry name" value="DM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001352998.2"/>
        </source>
    </evidence>
    <sequence length="188" mass="21029" checksum="15A5172614A4B31D" modified="2008-10-14" version="2">MTDAFLLGLLVFSAAVAVLLEPLLATRDFEIRWRTINCTAIDPATTAAFHCLIVELPKRQGNFLNTRLLLNRPVSKMWVEMSVGQVGGTGTVQNLMKVRVDACHLIGYRSKNRILNAVLGKLLQSGNYPKTCPLLGNVNYTSTHFALNPDHFPAYMPDMKFNTKLVFYLSKNITLIRASVDAEVMRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q29E48</accession>
    <accession>A0A6I8UB36</accession>
    <name>Q29E48_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Pgant6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="15">MV-25-SWS-2005</strain>
            <tissue evidence="15">Whole body</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_001352715.1">
        <property type="nucleotide sequence ID" value="XM_001352679.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29E48"/>
    <dbReference type="SMR" id="Q29E48"/>
    <dbReference type="FunCoup" id="Q29E48">
        <property type="interactions" value="901"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29E48"/>
    <dbReference type="GeneID" id="4812047"/>
    <dbReference type="KEGG" id="dpo:4812047"/>
    <dbReference type="eggNOG" id="KOG3736">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013477_0_1_1"/>
    <dbReference type="InParanoid" id="Q29E48"/>
    <dbReference type="OMA" id="KTQFWEL"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0075267">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23462">
        <property type="entry name" value="beta-trefoil_Ricin_Pgant9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000029">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF134">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 11" id="KW-0430">Lectin</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="13">
        <location>
            <begin position="526"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="79"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_001352715.1"/>
        </source>
    </evidence>
    <sequence length="676" mass="78427" checksum="40272D158BC0498E" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q29FL3</accession>
    <accession>A0A6I8UES2</accession>
    <name>Q29FL3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Torsin-1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC4814191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="2">MV-25-SWS-2005</strain>
            <tissue evidence="2">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354334.2">
        <property type="nucleotide sequence ID" value="XM_001354298.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29FL3"/>
    <dbReference type="FunCoup" id="Q29FL3">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="GeneID" id="4814191"/>
    <dbReference type="KEGG" id="dpo:4814191"/>
    <dbReference type="eggNOG" id="ENOG502SDUG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067926_0_0_1"/>
    <dbReference type="InParanoid" id="Q29FL3"/>
    <dbReference type="OMA" id="CKTVRAK"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078500">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29FL3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_001354334.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="39874" checksum="72FD4984388F0FF9" modified="2008-10-14" version="2">MAADKTPPITAGWKRAKHRLLKRRLNDLHQCKASLGSHRHWGHSRVRRLIADDKYDAQQSPPNHTWMFQSCWCIVLLALGVFICGFGCYYDGENVSNFMLNYSKQYQAIIHNKQNYCDPTLSLNEMFGHIRRHVLNQETALDQLEQSLSKPQFQSISLLGSSGVGKSLTARLLRELFPWPENVHALTKSDVSDLVRINSVLTKLDFCGRNLILVDNMNSTDQEIVQRVNDLVSSFGDIAGNKNTHKVNLKRLSIVYIFNINRFLGETSVEHFATVLQLPDNQVIQFDTLEPQHLESCIRHEADLENLHLDQKYVESMINTTDFKMSGCKTLRAKVLIYGQPKSNYQKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-10-13" modified="2025-06-18" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q29GB8</accession>
    <name>NAAT1_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sodium-dependent nutrient amino acid transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NAAT1</name>
        <name type="ORF">GA16959</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Unusual broad substrate spectrum amino acid:sodium cotransporter that promotes absorption of the D isomers of essential amino acids. Neutral amino acids are the preferred substrates, especially methionine and phenylalanine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="CH379064">
        <property type="protein sequence ID" value="EAL32192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_001355135.1">
        <property type="nucleotide sequence ID" value="XM_001355099.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29GB8"/>
    <dbReference type="SMR" id="Q29GB8"/>
    <dbReference type="FunCoup" id="Q29GB8">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="GlyCosmos" id="Q29GB8">
        <property type="glycosylation" value="2 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="EnsemblMetazoa" id="FBtr0289297">
        <property type="protein sequence ID" value="FBpp0287735"/>
        <property type="gene ID" value="FBgn0076973"/>
    </dbReference>
    <dbReference type="GeneID" id="4815002"/>
    <dbReference type="KEGG" id="dpo:4815002"/>
    <dbReference type="CTD" id="31457"/>
    <dbReference type="eggNOG" id="KOG3660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006855_9_5_1"/>
    <dbReference type="InParanoid" id="Q29GB8"/>
    <dbReference type="OMA" id="LQNFCDD"/>
    <dbReference type="PhylomeDB" id="Q29GB8"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076973">
        <property type="expression patterns" value="Expressed in insect adult head and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000305"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042943">
        <property type="term" value="F:D-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042940">
        <property type="term" value="P:D-amino acid transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015804">
        <property type="term" value="P:neutral amino acid transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd10324">
        <property type="entry name" value="SLC6sbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF321">
        <property type="entry name" value="SODIUM-DEPENDENT NUTRIENT AMINO ACID TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0029">Amino-acid transport</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0739">Sodium transport</keyword>
    <keyword id="KW-0769">Symport</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000386586" description="Sodium-dependent nutrient amino acid transporter 1">
        <location>
            <begin position="1"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=1" evidence="3">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=2" evidence="3">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=3" evidence="3">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=4" evidence="3">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
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            <begin position="241"/>
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        </location>
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        <location>
            <begin position="270"/>
            <end position="290"/>
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        <location>
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            <end position="339"/>
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        <location>
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        <location>
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        <location>
            <begin position="459"/>
            <end position="479"/>
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    <feature type="transmembrane region" description="Helical; Name=11" evidence="3">
        <location>
            <begin position="486"/>
            <end position="506"/>
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        <location>
            <begin position="528"/>
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        <location>
            <begin position="565"/>
            <end position="585"/>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="10"/>
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        <location>
            <begin position="32"/>
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        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
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        <location>
            <position position="202"/>
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        <location>
            <position position="205"/>
        </location>
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    <evidence type="ECO:0000250" key="1"/>
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        <source>
            <dbReference type="UniProtKB" id="Q9W4C5"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="EAL32192.1"/>
        </source>
    </evidence>
    <sequence length="653" mass="72810" checksum="C2E89CBC9DF863BB" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q29IE4</accession>
    <accession>A0A6I8UHM3</accession>
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        <submittedName>
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        <name evidence="3" type="primary">Vlet</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <strain evidence="3">MV-25-SWS-2005</strain>
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    </reference>
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            <begin position="43"/>
            <end position="76"/>
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        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001355650.2"/>
        </source>
    </evidence>
    <sequence length="243" mass="27207" checksum="7D82E310C24C9167" modified="2008-10-14" version="2">MSINWIKITERAREGIKIINALPYDTFNTVLWYTHRQMSPSATAAAPTSTVGTSVTTTERADSIAEDTPTSSNEPEYTLEELERLVGVPRADFLLLIKTFSYILRRISTFIIKPSLLQRELREKLQLEDEAKIDAILRLWVREATPIMENLASPRYESNVVEDVAWKLHVEVSSHCQQRDKTPIGVLQLRTAAGEDISSEMTHPELLQLYNQFEQIQAELDATLAMTETAPASGAASSSGSVQ</sequence>
</entry>
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    <accession>Q29JT6</accession>
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        <submittedName>
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        <name evidence="7" type="primary">LOC4816198</name>
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        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
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            <taxon>Diptera</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <strain evidence="7">MV-25-SWS-2005</strain>
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            <begin position="1"/>
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        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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        <source>
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            <dbReference type="Pfam" id="PF00135"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
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        <source>
            <dbReference type="RefSeq" id="XP_001355816.1"/>
        </source>
    </evidence>
    <sequence length="688" mass="75482" checksum="4EA0392B5DA6ADD1" modified="2006-04-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KH1</accession>
    <accession>A0A6I8UIU7</accession>
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        <submittedName>
            <fullName evidence="3">Protein ABHD13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Bem46</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
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        <property type="interactions" value="1171"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KH1"/>
    <dbReference type="ESTHER" id="drome-BEM46">
        <property type="family name" value="ABHD13-BEM46"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
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        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
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        <property type="expression patterns" value="baseline"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="1">
        <location>
            <begin position="221"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356145.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="38313" checksum="4C49DB1283596AB2" modified="2006-04-04" version="1">MKEVGIALPKSRGVGVGIVAGFLLCFIFYYFYGGYMTFLLFASILLLIFYYAQDLLLYHPDLPTNSRIYIPIPTMHNLPHITVSINTPDGVTLHAFWISQPEERCKLVPTLLYFHGNAGNMGHRMQNVWGIYHHLHCNILMVEYRGYGLSTGVPTERGLVTDARSAIDYLHTRHDLDHSQLILFGRSLGGAVVVDVAADAVYGQKLMCAIVENTFSSIPEMAVELVHPSVKYIPNLLYKNKYHSLNKISKCSVPFLFISGLADNLVPPRMMRALYTKCGSELKRLLEFPGGSHNDTWIVDGYYQAIGQFLAELQQAPLPLQKPPEKSNVWVELEHKIIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q29KQ7</accession>
    <accession>A0A6I8UIJ7</accession>
    <name>Q29KQ7_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356058.1">
        <property type="nucleotide sequence ID" value="XM_001356022.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29KQ7"/>
    <dbReference type="FunCoup" id="Q29KQ7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29KQ7"/>
    <dbReference type="GeneID" id="4816490"/>
    <dbReference type="KEGG" id="dpo:4816490"/>
    <dbReference type="eggNOG" id="KOG3213">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060610_0_0_1"/>
    <dbReference type="InParanoid" id="Q29KQ7"/>
    <dbReference type="OMA" id="FIHCPSD"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0074359">
        <property type="expression patterns" value="Expressed in male reproductive system and 1 other cell type or tissue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040441">
        <property type="entry name" value="CFA20/CFAP20DC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12458">
        <property type="entry name" value="ORF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFA20" evidence="2">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356058.1"/>
        </source>
    </evidence>
    <sequence length="274" mass="31015" checksum="BE7B17B94707E740" modified="2006-04-04" version="1">MYRSAYQKGFLTILYSVGSSPLQQWSSYTKNGYIKRIYDDDIKSLVLEIIGSNVSTAFIHCPSECKEQLGIKLPFLVLLIKNMHKYFCFEVKIQDDQRFMRRFRVSNFQSKTSVKPFCTAMPMGMSPGWNQIQFNLADFTRRAYGSNYLETVSLQIHANVRIRRVYFADKLYTEAELPNDYRLMGKPKDLKKPEKQFKVPPTARPPSPLNTARGEGPVKAPQPEAPVQESEPEAAPELKKAPSPTVSVGVVSAAPEPAAEEPPTQTEATEEAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LW9</accession>
    <accession>A0A6I8UKC4</accession>
    <name>Q29LW9_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-methylacyl-CoA racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Amacr</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoA-transferase III family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001356860.1">
        <property type="nucleotide sequence ID" value="XM_001356824.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LW9"/>
    <dbReference type="SMR" id="Q29LW9"/>
    <dbReference type="FunCoup" id="Q29LW9">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LW9"/>
    <dbReference type="GeneID" id="4817280"/>
    <dbReference type="KEGG" id="dpo:4817280"/>
    <dbReference type="eggNOG" id="KOG3957">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_5_0_1"/>
    <dbReference type="InParanoid" id="Q29LW9"/>
    <dbReference type="OMA" id="VVIDPFR"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081682">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008111">
        <property type="term" value="F:alpha-methylacyl-CoA racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008206">
        <property type="term" value="P:bile acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050509">
        <property type="entry name" value="CoA-transferase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228:SF5">
        <property type="entry name" value="ALPHA-METHYLACYL-COA RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228">
        <property type="entry name" value="SUCCINYL-COA--D-CITRAMALATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001356860.1"/>
        </source>
    </evidence>
    <sequence length="373" mass="41416" checksum="60C6873EE0B76E02" modified="2006-04-04" version="1">MPLKGIKVLEFVGLAPGPLCGKILTDFGATVTRIDKILDNQLDVLQHGKRTMCVNMKDPEGQQLVRRLAKKSDVLIEPFRPGVMEKLHLGPAELCGENPRLIYARLTGFGQQGRLAARAGHDLNYAAISGVLSMLGRRDEKPTAPINLLADFAGGSVMCALGICLALLERHNSGRGQVVDAAMVEGAAYVASWLFLSRDLHIWGKQRGDNILDGGAYFYDNYETKDGLYMSVGALEPQFFELLRQRLELPEDLPQFGDEHQERGRRLLTEAFLTKTQAQWSKIFEDIDACVYPVLDYTKVHQHAHNKERQSFGLLGDAVAPRPAPRLSRTPGKLPKAESTKQEELIGDLELKPEELKRLLESGVLTLPDRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q29LX6</accession>
    <accession>A0A6I8UK93</accession>
    <name>Q29LX6_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM107B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4816935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001356853.1">
        <property type="nucleotide sequence ID" value="XM_001356817.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29LX6"/>
    <dbReference type="FunCoup" id="Q29LX6">
        <property type="interactions" value="813"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29LX6"/>
    <dbReference type="GeneID" id="4816935"/>
    <dbReference type="KEGG" id="dpo:4816935"/>
    <dbReference type="eggNOG" id="ENOG502S71T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117321_0_0_1"/>
    <dbReference type="InParanoid" id="Q29LX6"/>
    <dbReference type="OMA" id="DTMMSMS"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0081690">
        <property type="expression patterns" value="Expressed in insect adult head and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q29LX6">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009533">
        <property type="entry name" value="FAM107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768">
        <property type="entry name" value="DOWN REGULATED IN RENAL CARCINOMA 1/TU3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768:SF5">
        <property type="entry name" value="FI14214P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06625">
        <property type="entry name" value="DUF1151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001356853.1"/>
        </source>
    </evidence>
    <sequence length="181" mass="20074" checksum="B4736D9CCE9789B7" modified="2006-04-04" version="1">MQRPTPNISTSIMSRMSVFENTAFRHSSAESENEAGTTMMSMSSSPPATPTGVQTDSEGLILPKKLINPCIENTDRKELHRELKFNARIGKSVLNQKSELQRAYDKQKERHAAAEHHSPDAELVGGIPGLKGELGRVILERAQKHEAAAQKQDADEAEDRQYVNPEYLNVRAKLRTANGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-04-04" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NN5</accession>
    <accession>A0A6I8VY27</accession>
    <name>Q29NN5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Tmtc2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="20">MV-25-SWS-2005</strain>
            <tissue evidence="20">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers mannosyl residues to the hydroxyl group of serine or threonine residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMTC family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="6"/>
    <dbReference type="RefSeq" id="XP_033235878.1">
        <property type="nucleotide sequence ID" value="XM_033379987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NN5"/>
    <dbReference type="SMR" id="Q29NN5"/>
    <dbReference type="FunCoup" id="Q29NN5">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4817768"/>
    <dbReference type="eggNOG" id="KOG1124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011615_4_0_1"/>
    <dbReference type="InParanoid" id="Q29NN5"/>
    <dbReference type="OMA" id="TQIFYND"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0078129">
        <property type="expression patterns" value="Expressed in insect adult head"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013618">
        <property type="entry name" value="TMTC_DUF1736"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052384">
        <property type="entry name" value="TMTC_O-mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44216">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44216:SF3">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08409">
        <property type="entry name" value="TMTC_DUF1736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="556"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1736" evidence="18">
        <location>
            <begin position="274"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="466"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="466"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_033235878.1"/>
        </source>
    </evidence>
    <sequence length="961" mass="104972" checksum="4887E1E7C3A88DAD" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-11-13" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q29NU5</accession>
    <accession>I5AMN0</accession>
    <name>GLT25_DROPS</name>
    <protein>
        <recommendedName>
            <fullName>Glycosyltransferase 25 family member</fullName>
            <ecNumber>2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GA16561</name>
    </gene>
    <organism>
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Genome Res." volume="15" first="1" last="18">
            <title>Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.</title>
            <authorList>
                <person name="Richards S."/>
                <person name="Liu Y."/>
                <person name="Bettencourt B.R."/>
                <person name="Hradecky P."/>
                <person name="Letovsky S."/>
                <person name="Nielsen R."/>
                <person name="Thornton K."/>
                <person name="Hubisz M.J."/>
                <person name="Chen R."/>
                <person name="Meisel R.P."/>
                <person name="Couronne O."/>
                <person name="Hua S."/>
                <person name="Smith M.A."/>
                <person name="Zhang P."/>
                <person name="Liu J."/>
                <person name="Bussemaker H.J."/>
                <person name="van Batenburg M.F."/>
                <person name="Howells S.L."/>
                <person name="Scherer S.E."/>
                <person name="Sodergren E."/>
                <person name="Matthews B.B."/>
                <person name="Crosby M.A."/>
                <person name="Schroeder A.J."/>
                <person name="Ortiz-Barrientos D."/>
                <person name="Rives C.M."/>
                <person name="Metzker M.L."/>
                <person name="Muzny D.M."/>
                <person name="Scott G."/>
                <person name="Steffen D."/>
                <person name="Wheeler D.A."/>
                <person name="Worley K.C."/>
                <person name="Havlak P."/>
                <person name="Durbin K.J."/>
                <person name="Egan A."/>
                <person name="Gill R."/>
                <person name="Hume J."/>
                <person name="Morgan M.B."/>
                <person name="Miner G."/>
                <person name="Hamilton C."/>
                <person name="Huang Y."/>
                <person name="Waldron L."/>
                <person name="Verduzco D."/>
                <person name="Clerc-Blankenburg K.P."/>
                <person name="Dubchak I."/>
                <person name="Noor M.A."/>
                <person name="Anderson W."/>
                <person name="White K.P."/>
                <person name="Clark A.G."/>
                <person name="Schaeffer S.W."/>
                <person name="Gelbart W."/>
                <person name="Weinstock G.M."/>
                <person name="Gibbs R.A."/>
            </authorList>
            <dbReference type="PubMed" id="15632085"/>
            <dbReference type="DOI" id="10.1101/gr.3059305"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>MV2-25 / Tucson 14011-0121.94</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 25 family.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-"/>
    <dbReference type="EMBL" id="CH379058">
        <property type="protein sequence ID" value="EIM52215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q29NU5"/>
    <dbReference type="SMR" id="Q29NU5"/>
    <dbReference type="FunCoup" id="Q29NU5">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q29NU5"/>
    <dbReference type="EnsemblMetazoa" id="FBtr0301991">
        <property type="protein sequence ID" value="FBpp0291201"/>
        <property type="gene ID" value="FBgn0076576"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4818107"/>
    <dbReference type="eggNOG" id="KOG4179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024037_2_0_1"/>
    <dbReference type="InParanoid" id="Q29NU5"/>
    <dbReference type="OMA" id="QRVYHYV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0076576">
        <property type="expression patterns" value="Expressed in female reproductive system and 2 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050211">
        <property type="term" value="F:procollagen galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06532">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002654">
        <property type="entry name" value="Glyco_trans_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF53">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01755">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00014">
        <property type="entry name" value="ER_TARGET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000309550" description="Glycosyltransferase 25 family member">
        <location>
            <begin position="22"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Prevents secretion from ER" evidence="2">
        <location>
            <begin position="623"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="626" mass="72334" checksum="85B74CDB5474ECDE" modified="2007-11-13" version="2" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q2LZN3</accession>
    <accession>A0A6I8UDF1</accession>
    <name>Q2LZN3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TM9SF3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="9">MV-25-SWS-2005</strain>
            <tissue evidence="9">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353740.2">
        <property type="nucleotide sequence ID" value="XM_001353704.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2LZN3"/>
    <dbReference type="FunCoup" id="Q2LZN3">
        <property type="interactions" value="2183"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2LZN3"/>
    <dbReference type="GeneID" id="4812975"/>
    <dbReference type="KEGG" id="dpo:4812975"/>
    <dbReference type="eggNOG" id="KOG1277">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010714_0_2_1"/>
    <dbReference type="InParanoid" id="Q2LZN3"/>
    <dbReference type="OMA" id="DAPCRVN"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070477">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="Q2LZN3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF41">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035543881" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="31"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="483"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="552"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_001353740.2"/>
        </source>
    </evidence>
    <sequence length="591" mass="67811" checksum="CC79EB4443785FCC" modified="2008-10-14" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-21" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q2M0M1</accession>
    <accession>A0A6I8UCW8</accession>
    <name>Q2M0M1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ubiquitin-conjugating enzyme E2 C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">vih</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="7">MV-25-SWS-2005</strain>
            <tissue evidence="7">Whole body</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001353402.1">
        <property type="nucleotide sequence ID" value="XM_001353366.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2M0M1"/>
    <dbReference type="FunCoup" id="Q2M0M1">
        <property type="interactions" value="911"/>
    </dbReference>
    <dbReference type="STRING" id="46245.Q2M0M1"/>
    <dbReference type="GeneID" id="4813733"/>
    <dbReference type="KEGG" id="dpo:4813733"/>
    <dbReference type="eggNOG" id="KOG0421">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_9_3_1"/>
    <dbReference type="InParanoid" id="Q2M0M1"/>
    <dbReference type="OMA" id="PKDNHAV"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0070548">
        <property type="expression patterns" value="Expressed in female reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23791">
        <property type="entry name" value="UBCc_UBE2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000085">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="30"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_001353402.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="19653" checksum="8126247DB922CFA0" modified="2006-02-21" version="1">MAQNISPEQSGGAGGTKHTDDSMPVKDNHAVSKRLQKELMNLMLASERGISAFPDGENIFKWVGTIDGPSNTVYAGQTYRLSLDFPNSYPYAAPVVKFLTSCFHPNVDLQGSICLDILKDKWSALYDVRTILLSIQSLLGEPNNESPLNAQAAMMWNDQKEYKKYLDGFYEKHKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-01-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0R3P4J5</accession>
    <accession>A0A6I8WC34</accession>
    <name>A0A0R3P4J5_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein 188</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Cnep1r2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="12">MV-25-SWS-2005</strain>
            <tissue evidence="12">Whole body</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNEP1R1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_033240953.1">
        <property type="nucleotide sequence ID" value="XM_033385062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0R3P4J5"/>
    <dbReference type="FunCoup" id="A0A0R3P4J5">
        <property type="interactions" value="817"/>
    </dbReference>
    <dbReference type="STRING" id="46245.A0A0R3P4J5"/>
    <dbReference type="KEGG" id="dpo:6900691"/>
    <dbReference type="InParanoid" id="A0A0R3P4J5"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Bgee" id="FBgn0245233">
        <property type="expression patterns" value="Expressed in male reproductive system and 3 other cell types or tissues"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071595">
        <property type="term" value="C:Nem1-Spo7 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019168">
        <property type="entry name" value="NEP1-R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20996:SF1">
        <property type="entry name" value="NUCLEAR ENVELOPE PHOSPHATASE-REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09771">
        <property type="entry name" value="Tmemb_18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_033240953.1"/>
        </source>
    </evidence>
    <sequence length="136" mass="15435" checksum="817DDBB4B77365BA" modified="2016-01-20" version="1">MEPSACEGELEPTPKEDLKAFERRLTEVVLSYRPPTFRWRILLSAMSMCTAIGAWYWLRDPRTTVVPLTESLWIHPIFTAATVTLIVLFILGIQKLVIAPQIITSRTRNVLGDFNMSCDDTGKLILKPRQSNNNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UC99</accession>
    <name>A0A6I8UC99_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sstn</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="3">MV-25-SWS-2005</strain>
            <tissue evidence="3">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001353178.4">
        <property type="nucleotide sequence ID" value="XM_001353142.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UC99"/>
    <dbReference type="FunCoup" id="A0A6I8UC99">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812786"/>
    <dbReference type="InParanoid" id="A0A6I8UC99"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="423"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_001353178.4"/>
        </source>
    </evidence>
    <sequence length="626" mass="69655" checksum="6B8A3B3C488E69F4" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UE53</accession>
    <name>A0A6I8UE53_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Elp5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="11">MV-25-SWS-2005</strain>
            <tissue evidence="11">Whole body</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354038.2">
        <property type="nucleotide sequence ID" value="XM_001354002.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UE53"/>
    <dbReference type="FunCoup" id="A0A6I8UE53">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4812793"/>
    <dbReference type="InParanoid" id="A0A6I8UE53"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UE53">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_001354038.2"/>
        </source>
    </evidence>
    <sequence length="276" mass="30750" checksum="46C389523192F07D" modified="2020-08-12" version="1">MLSNLLVTKQKVVFIIDELNRERIALRFIDSLLHEQGQEAGTIKTLPIGNLRHVSSYEALLNECGNNNNNNQSGEAADFKSSPTTDTGSSSTKAEYNIILPTLADLLCYQTPAYIFSFLNRLRRSEHVRRVFLWASPQHLQHHHAQYILAGCEYLAELILRLESETLLSLISRKPGGGVSNKRYACRITKTEFQVTPLDGPAAVGSPKQPSPEADQTAEPASSTFKIELDEDEVVARNALTLPYERTSEPAEGNIIYTPDADDDFDDEDPDEDLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFN3</accession>
    <name>A0A6I8UFN3_DROPS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisomal targeting signal 1 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">PTS1-BP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Peroxin-5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Pex5</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="19">MV-25-SWS-2005</strain>
            <tissue evidence="19">Whole body</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">In addition to promoting peroxisomal translocation of proteins containing a PTS1 peroxisomal targeting signal, mediates peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal via its interaction with PEX7. Interaction with PEX7 only takes place when PEX7 is associated with cargo proteins containing a PTS2 peroxisomal targeting signal. PEX7 along with PTS2-containing cargo proteins are then translocated through the PEX13-PEX14 docking complex together with PEX5.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_001354741.2">
        <property type="nucleotide sequence ID" value="XM_001354705.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFN3"/>
    <dbReference type="FunCoup" id="A0A6I8UFN3">
        <property type="interactions" value="606"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815260"/>
    <dbReference type="InParanoid" id="A0A6I8UFN3"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="ExpressionAtlas" id="A0A6I8UFN3">
        <property type="expression patterns" value="baseline"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000034">
        <property type="entry name" value="Peroxisomal biogenesis factor 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF0">
        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 17" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="17">
        <location>
            <begin position="278"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="17">
        <location>
            <begin position="392"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="17">
        <location>
            <begin position="426"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_001354741.2"/>
        </source>
    </evidence>
    <sequence length="552" mass="62271" checksum="5887D197DB940940" modified="2020-08-12" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFR1</accession>
    <name>A0A6I8UFR1_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein Cp7Fb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Cp7Fb</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
            <tissue evidence="4">Whole body</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_001354720.4">
        <property type="nucleotide sequence ID" value="XM_001354684.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A6I8UFR1"/>
    <dbReference type="FunCoup" id="A0A6I8UFR1">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="KEGG" id="dpo:4815200"/>
    <dbReference type="InParanoid" id="A0A6I8UFR1"/>
    <dbReference type="Proteomes" id="UP000001819">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5026152923" evidence="2">
        <location>
            <begin position="20"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_001354720.4"/>
        </source>
    </evidence>
    <sequence length="506" mass="56366" checksum="A822161B8A9F4E07" modified="2020-08-12" version="1">MRQLITLLGLVVVLGLVQSAPVEVVYTGEGCACPTQLGYQLNVPNPPKPKKYGTSDYGYRVEKPVQTKAKISYSFDFTVEQPRTPPPPKYPESKLYAKVDRVTVNKGDCAAKHTSSCSCSSCSSPKQRYVAPGPGPDPGPRSPNAIRRRRDLRTPGSLIRRRLMRQQQLQERPPRYVKLYHKDLAAQQQHQQQKLKLFGLMPAESHEPSPKTKRKTKKSPAGWLGLGRRLAKRDIKGEFDLLERERAQIDLTAPEIIQFMPETLNPNFQPGQCHMGCGAITAVGPPPPQPLPPATEEAGKEFDPTEDQTELPVTTEQQNTIPAKRAALGYYPLQIPSPLGLPLDDYKYVDDSQLRSLLSTTSVPDPLEQGSTPLQFAGDLESVTRRGYTSALLGSGGGYPVQHVSDSQLDRLISEIVYPHPHYLESSTHYPEPHQKRQTTDFLKKLIDGRLGGWGFDSATEPDHTLHADYSTTPAKTYGYNSHVQNGYSIDTYNVPPISEYLRAWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-08-12" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A6I8UFT0</accession>
    <name>A0A6I8UFT0_DROPS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trithorax group protein osa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC4814445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Drosophila pseudoobscura pseudoobscura</name>
        <name type="common">Fruit fly</name>
        <dbReference type="NCBI Taxonomy" id="46245"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Ecdysozoa</taxon>
            <taxon>Arthropoda</taxon>
            <taxon>Hexapoda</taxon>
            <taxon>Insecta</taxon>
            <taxon>Pterygota</taxon>
            <taxon>Neoptera</taxon>
            <taxon>Endopterygota</taxon>
            <taxon>Diptera</taxon>
            <taxon>Brachycera</taxon>
            <taxon>Muscomorpha</taxon>
            <taxon>Ephydroidea</taxon>
            <taxon>Drosophilidae</taxon>
            <taxon>Drosophila</taxon>
            <taxon>Sophophora</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="4">MV-25-SWS-2005</strain>
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